LOCUS       DAXHMX010000001        51277 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHMX010000001 DAXHMX010000000
VERSION     DAXHMX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 51277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.001"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(38..703)
                     /locus_tag="ACFM4S_000001"
     CDS             complement(38..703)
                     /locus_tag="ACFM4S_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049776.1"
                     /translation="MENIEEYALLSPEALLERLDTVLSIRIGGKGWESSMTANFAQTL
                     VEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELNFKISEGSTRVESDFKETLVRFGR
                     DMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYLKQVASEGYQTERLYNAVDRLAES
                     QERITSAILKGARGADFVQIGRRSYSREDISEANRRAERVPYGAELVSDGNFTAVLSD
                     IGD"
     gene            complement(711..908)
                     /locus_tag="ACFM4S_000002"
     CDS             complement(711..908)
                     /locus_tag="ACFM4S_000002"
                     /inference="COORDINATES: protein motif:HMM:NF019096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HFC6049777.1"
                     /translation="MKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALNTPYGSIR
                     TKLDGRSNPDNLVCNRYKVLL"
     gene            complement(930..1103)
                     /locus_tag="ACFM4S_000003"
                     /pseudo
     CDS             complement(930..1103)
                     /locus_tag="ACFM4S_000003"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            complement(1311..1703)
                     /locus_tag="ACFM4S_000004"
     CDS             complement(1311..1703)
                     /locus_tag="ACFM4S_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6049778.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(1820..2230)
                     /locus_tag="ACFM4S_000005"
     CDS             complement(1820..2230)
                     /locus_tag="ACFM4S_000005"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6049779.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(2244..2627)
                     /locus_tag="ACFM4S_000006"
     CDS             complement(2244..2627)
                     /locus_tag="ACFM4S_000006"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6049780.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(3092..4390)
                     /locus_tag="ACFM4S_000007"
     CDS             complement(3092..4390)
                     /locus_tag="ACFM4S_000007"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6049781.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAILAYYNVQLQHLHNAEPVKAEDVMAVIEKVALRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(4494..5645)
                     /locus_tag="ACFM4S_000008"
     CDS             complement(4494..5645)
                     /locus_tag="ACFM4S_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6049782.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTSERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            5980..7359
                     /gene="norM"
                     /locus_tag="ACFM4S_000009"
     CDS             5980..7359
                     /gene="norM"
                     /locus_tag="ACFM4S_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6049783.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            7537..8577
                     /gene="murB"
                     /locus_tag="ACFM4S_000010"
     CDS             7537..8577
                     /gene="murB"
                     /locus_tag="ACFM4S_000010"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6049784.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            8589..9239
                     /locus_tag="ACFM4S_000011"
     CDS             8589..9239
                     /locus_tag="ACFM4S_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6049785.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            9294..10070
                     /locus_tag="ACFM4S_000012"
     CDS             9294..10070
                     /locus_tag="ACFM4S_000012"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6049786.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPALDFAPDDSVGKIIRTVCDNGLSL"
     gene            10121..10687
                     /locus_tag="ACFM4S_000013"
     CDS             10121..10687
                     /locus_tag="ACFM4S_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6049787.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            10707..11597
                     /locus_tag="ACFM4S_000014"
     CDS             10707..11597
                     /locus_tag="ACFM4S_000014"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6049788.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIRESKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            11622..12380
                     /locus_tag="ACFM4S_000015"
     CDS             11622..12380
                     /locus_tag="ACFM4S_000015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6049789.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(12435..13100)
                     /locus_tag="ACFM4S_000016"
     CDS             complement(12435..13100)
                     /locus_tag="ACFM4S_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6049790.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYRAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSRQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(13301..14074)
                     /gene="folE2"
                     /locus_tag="ACFM4S_000017"
     CDS             complement(13301..14074)
                     /gene="folE2"
                     /locus_tag="ACFM4S_000017"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6049791.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEALDFAQLRRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            14266..15423
                     /locus_tag="ACFM4S_000018"
     CDS             14266..15423
                     /locus_tag="ACFM4S_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6049792.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPMDCWLVLRGIKTLALRMNAHYQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSVGIEDVDDIWNDISAALDTTL"
     gene            complement(15486..16487)
                     /gene="hemB"
                     /locus_tag="ACFM4S_000019"
     CDS             complement(15486..16487)
                     /gene="hemB"
                     /locus_tag="ACFM4S_000019"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6049793.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMQGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTLIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(16670..16954)
                     /gene="yhbY"
                     /locus_tag="ACFM4S_000020"
     CDS             complement(16670..16954)
                     /gene="yhbY"
                     /locus_tag="ACFM4S_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6049794.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            17064..17684
                     /locus_tag="ACFM4S_000021"
     CDS             17064..17684
                     /locus_tag="ACFM4S_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6049795.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            17748..19715
                     /gene="ftsH"
                     /locus_tag="ACFM4S_000022"
     CDS             17748..19715
                     /gene="ftsH"
                     /locus_tag="ACFM4S_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6049796.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQIA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            19937..20368
                     /locus_tag="ACFM4S_000023"
     CDS             19937..20368
                     /locus_tag="ACFM4S_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6049797.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            20361..20639
                     /locus_tag="ACFM4S_000024"
     CDS             20361..20639
                     /locus_tag="ACFM4S_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6049798.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            20692..21270
                     /gene="pth"
                     /locus_tag="ACFM4S_000025"
     CDS             20692..21270
                     /gene="pth"
                     /locus_tag="ACFM4S_000025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6049799.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            21331..21537
                     /locus_tag="ACFM4S_000026"
     CDS             21331..21537
                     /locus_tag="ACFM4S_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6049800.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            21534..21896
                     /locus_tag="ACFM4S_000027"
     CDS             21534..21896
                     /locus_tag="ACFM4S_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6049801.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            22127..23542
                     /locus_tag="ACFM4S_000028"
     CDS             22127..23542
                     /locus_tag="ACFM4S_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC6049802.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            23661..24170
                     /locus_tag="ACFM4S_000029"
     CDS             23661..24170
                     /locus_tag="ACFM4S_000029"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6049803.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            24333..25715
                     /locus_tag="ACFM4S_000030"
     CDS             24333..25715
                     /locus_tag="ACFM4S_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6049804.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSSMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(25735..26490)
                     /locus_tag="ACFM4S_000031"
     CDS             complement(25735..26490)
                     /locus_tag="ACFM4S_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6049805.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(26500..27216)
                     /locus_tag="ACFM4S_000032"
     CDS             complement(26500..27216)
                     /locus_tag="ACFM4S_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6049806.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMVIAVAVALVRIMPAGGIARKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(27206..28033)
                     /locus_tag="ACFM4S_000033"
     CDS             complement(27206..28033)
                     /locus_tag="ACFM4S_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6049807.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            28318..29226
                     /locus_tag="ACFM4S_000034"
     CDS             28318..29226
                     /locus_tag="ACFM4S_000034"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6049808.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            29306..32920
                     /gene="recB"
                     /locus_tag="ACFM4S_000035"
     CDS             29306..32920
                     /gene="recB"
                     /locus_tag="ACFM4S_000035"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6049809.1"
                     /translation="MSAPIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAALKKGKTPDAAAFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAGDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            32959..33318
                     /locus_tag="ACFM4S_000036"
     CDS             32959..33318
                     /locus_tag="ACFM4S_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6049810.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            33560..34039
                     /locus_tag="ACFM4S_000037"
     CDS             33560..34039
                     /locus_tag="ACFM4S_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6049811.1"
                     /translation="MKHILPLIAASALCISTASAHPAGKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMNIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(34110..35489)
                     /gene="radA"
                     /locus_tag="ACFM4S_000038"
     CDS             complement(34110..35489)
                     /gene="radA"
                     /locus_tag="ACFM4S_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6049812.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICREDRD"
     gene            complement(35678..36742)
                     /gene="hemE"
                     /locus_tag="ACFM4S_000039"
     CDS             complement(35678..36742)
                     /gene="hemE"
                     /locus_tag="ACFM4S_000039"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6049813.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDEAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(36817..38034)
                     /locus_tag="ACFM4S_000040"
     CDS             complement(36817..38034)
                     /locus_tag="ACFM4S_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC6049814.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETC"
     gene            complement(38031..39305)
                     /locus_tag="ACFM4S_000041"
     CDS             complement(38031..39305)
                     /locus_tag="ACFM4S_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6049815.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALG
                     LGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLD
                     GNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLEIAVSGLPLILDG
                     VLKPSVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDARTALMQRNSEVYQGDLNNAETAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTTAENQEAEQATSEPANENTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(39380..40141)
                     /locus_tag="ACFM4S_000042"
     CDS             complement(39380..40141)
                     /locus_tag="ACFM4S_000042"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6049816.1"
                     /translation="METAKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKIHIAVGQGSRRALERYLGRTV
                     IAPHDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(40159..40581)
                     /locus_tag="ACFM4S_000043"
     CDS             complement(40159..40581)
                     /locus_tag="ACFM4S_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6049817.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLSGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            complement(40832..41152)
                     /locus_tag="ACFM4S_000044"
     CDS             complement(40832..41152)
                     /locus_tag="ACFM4S_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6049818.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(41175..41801)
                     /gene="upp"
                     /locus_tag="ACFM4S_000045"
     CDS             complement(41175..41801)
                     /gene="upp"
                     /locus_tag="ACFM4S_000045"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6049819.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(41907..42218)
                     /gene="grxD"
                     /locus_tag="ACFM4S_000046"
     CDS             complement(41907..42218)
                     /gene="grxD"
                     /locus_tag="ACFM4S_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6049820.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(42319..42534)
                     /locus_tag="ACFM4S_000047"
     CDS             complement(42319..42534)
                     /locus_tag="ACFM4S_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC6049821.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(42560..43339)
                     /locus_tag="ACFM4S_000048"
     CDS             complement(42560..43339)
                     /locus_tag="ACFM4S_000048"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6049822.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQVLAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDTAFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(43344..43694)
                     /locus_tag="ACFM4S_000049"
     CDS             complement(43344..43694)
                     /locus_tag="ACFM4S_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6049823.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(43698..44675)
                     /locus_tag="ACFM4S_000050"
     CDS             complement(43698..44675)
                     /locus_tag="ACFM4S_000050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6049824.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHGEALAYLRDRGVAEPEEFLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(44709..45839)
                     /locus_tag="ACFM4S_000051"
     CDS             complement(44709..45839)
                     /locus_tag="ACFM4S_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6049825.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            46007..46708
                     /locus_tag="ACFM4S_000052"
     CDS             46007..46708
                     /locus_tag="ACFM4S_000052"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6049826.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            46851..48644
                     /gene="lepA"
                     /locus_tag="ACFM4S_000053"
     CDS             46851..48644
                     /gene="lepA"
                     /locus_tag="ACFM4S_000053"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6049827.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            48787..49806
                     /gene="lepB"
                     /locus_tag="ACFM4S_000054"
     CDS             48787..49806
                     /gene="lepB"
                     /locus_tag="ACFM4S_000054"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6049828.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(50025..50174)
                     /locus_tag="ACFM4S_000055"
     CDS             complement(50025..50174)
                     /locus_tag="ACFM4S_000055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049829.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(50220..51125)
                     /locus_tag="ACFM4S_000056"
     CDS             complement(50220..51125)
                     /locus_tag="ACFM4S_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6049830.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
BASE COUNT        12177 a        14613 c        12949 g        11538 t
ORIGIN      
        1 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atcccctata tcagataaaa
       61 cagcggtaaa attgccgtct gaaaccaact ctgcgccata cgggacacgc tctgcacgtc
      121 tatttgcctc cgatatatcc tccctactgt aggaacgtct gccgatttgc acgaaatcgg
      181 cacctctagc acccttcagg atggcggacg ttatccgttc ttgggattct gcaagacggt
      241 cgacggcatt atacagacgc tcggtctgat acccttccga agcaacctgc ttcaggtatt
      301 ccaaataacc gtaacccaac gcccctccga caagcagggt cgtcaacgct accagcgttg
      361 ccgaacggat tttagggggc atatcttgaa gcatatctct accgaaccga accaaagtct
      421 ctttaaagtc ggactctaca cgcgtagaac cctcggaaat cttgaagttc aactcatact
      481 gcttccgttt gtccgctgtc aaccgcttga ggttgtcatc cccgtattgg acggttgaca
      541 caaccctgta cagactgtcc tgtatttcga ccagcgtctg tgcaaagttg gcggtcatac
      601 tggattccca acccttgccg ccgattctga tactcaaaac ggtatccagg cgttccagca
      661 gggcttcggg agacagcagt gcgtattctt caatattttc cataggttga ttatagcaga
      721 accttatacc tgttacagac aaggttgtcg ggatttgaac gtccgtcgag cttggttcgt
      781 atagagccgt agggggtgtt tagggctttg gcggcagctt gtacgctggg gaacgtgagt
      841 gtttcgcctg tggcggtgtc gaggatggag acaggttttt ggttttgggg aggcgtattt
      901 tccttcattt taagtttccc gcactccgga cacccatact ttgactttat agctgtgtcg
      961 taatacaaaa gttggacaac cccgtgcgta ggacatctaa cactggaagg tttataaatg
     1021 ccactgaact ccaacaattc taaatgtgga aatttctctt gaaagcgtga tttggcggta
     1081 tcgaaagtca ttcgcttacc cataatttgt ctccataggt tatgtgcaaa tggattacac
     1141 aaccaaaata tagaaatttt caaatttttt gagacctacc tggaatcctg cgcaagcaca
     1201 atgtaaagaa aaatggtata cgtctttact aaatctttac ataaattaac tctaatcggg
     1261 atttgaacgt ccgtcgagct tggttcgtag gatttagggt gttgtgaacc ttacctatcg
     1321 tacccaagca ctttgtacct gtttgaaata aggttgtcgg gcgacgttcg acccttgagc
     1381 ctgtgattga tttgttggaa atgtacacct aaagcagcac ctgcggcagt aacgcttggg
     1441 aacgtgagtg tttcgttggt cgttgtgtcg aggatgagga ggggcttccc tacatttgga
     1501 ggtgtttggg tttttgacat cttagcacac tcaggacacc cgtactttga ctttataagg
     1561 gcattaaatg tcgaataacg aacgttcccg tgcataggac aattaacggt tactgggtaa
     1621 ttcacaccgt caaagccggt tagagagata ttagggaata tatcagtaag ttcagaagct
     1681 gcttggttga aagatttggc catttgagac tccaaaattg attgtcaaat ggatttaata
     1741 gcaaatttat aaaaatgtca aatttttcga gaagcacctg atagtggcga ggggtataca
     1801 ctgttaaaaa gtgtatacgt catttagtgc agatgtgaac cttatacctc cctgcaagta
     1861 aagtctctgt gtgaacccga ccttttatac ggtcagtgat agctgctggg tttataccca
     1921 atgaccttgc agcagatgct cttgagggga atgtaagtgt ttcgccagtt acagtatcct
     1981 ccaatactgt atttttagat aatttatcta ttgtatcttc tggaattttg tgggaattga
     2041 caccataaga tgcacatttt ggacacccac ttttagagtc caacatactt ttgaatgtag
     2101 agcaagtaac cctcccgtgt ataggacaga cgattaccgt cgggtaacga acgccattga
     2161 actccacaag attcaaatgt ggaaatttag aggttagctt agaaactgct tgagtaaatg
     2221 taagtgccat agtggttgtg aacttacttt accaacctat aacgatacct aatcaatcgg
     2281 tcaaacggag tgcgtccgct taaacgtaca gctaaagtgc tgttggagca accgataaat
     2341 ttactggcag ccagtgtact tgcaaactcg tagatttccc ctgtctctgt gtcctccaac
     2401 ctaatggcaa cggcgtttgg agagggcttt tcgggatttc gagtacattc aggacatcct
     2461 gttgaggatt tcatggcagc ttgtagattc ccgtaaacaa ccgacccatg caaagggcat
     2521 tggattgtta ccggttcgta gtttttagtg aacttcaaaa ccgtcttatc aggaaattta
     2581 ctggaaaacc ttaactgaac atcttgtagc gagagtgatt ttggcattat agacctttct
     2641 aaaatgaaag cattatacaa ccaaaatata gaaattttca aattttctct gaacgcactg
     2701 gtatccgatg gagctaaatg taaagaaaag ggtatatgtc cttactaaat ctttacacat
     2761 tttaactgta tagtgtttga caagcaacag gcataggcgc ataatgcacc cgtagttaga
     2821 tagatagtaa ggagataacc aatgacttag cacctgcccc cctctctctc gcgcaccccg
     2881 cccctcgctt gcggctaatg ggctttgcgc gaaatataca ctttcatctc ctgcatggct
     2941 taaccgccta tgcaatagta cacacatcaa caaatcaggc tggcacgtct tggcattggc
     3001 tgacgtacca ttgtagcccg aaatgttgat accctactat ttatgaccaa cggcggatta
     3061 gggcttcaga cggcaatttt actgcaaaac cttatgcgaa cggatgatgc agcacaatcg
     3121 tttcttctcg gtcggggccg gtggagacga tggcgaccgg cgcgccgcag acttcttcaa
     3181 tccgtttcaa atatgctttg gcattatcgg gcagttcctc ataatttttg atgccgaaag
     3241 tcgattcttt ccaaccctcc attgtttcgt aaatcggttt gcaggtttcc accgcatccg
     3301 aaccgcaagg caggatatcg gttttgccgc cgtcgggcaa ttcgtagccg acgcagatat
     3361 tgatggtttc aacgccgtcc attacatcga gtttggtaat gcacataccg gaaatgccgt
     3421 tgatttggat ggagcgtttc agggcggcgg catcaaacca gccgcagcgg cgtgcgcggc
     3481 cggttaccga gccgaattcg tgtccgcgtt ccgccaaacc tgcgcctact tcgtcgaaca
     3541 attcggtcgg gaacgggcct gaaccgacgc gcgtggtata ggctttgacg atgcccaaaa
     3601 cataatccag catttgaggg cctacgcccg cgcctgcgga agcggctccc gccagacagt
     3661 tggacgaggt aacgaaggga taagtgccgt agtcgatgtc caacaacgca ccttgcgcgc
     3721 cttcaaacag cagtttttcg ccgtttttgt ttttctcgtt caacacgcgg gacacgtcgg
     3781 taatcatcgg cgcaatgcgc agcgcgactt tttcgataac cgccatcacg tcttccgctt
     3841 taaccggctc ggcattgtgc agatgttgca gttggacatt gtaataggca aggatggcat
     3901 ccagtttttc acgcagtttt tcaggatgca gcaaatcggc ggcgcgaatg gcgcggcgtg
     3961 ccactttgtc ttcgtaggca gggccgatgc cgcggccggt cgtgccgatt ttgcctttgc
     4021 cgcgcgatgc ttcgcgggct tggtcgagcg cgatgtggta aggcaggatc agcgggcagg
     4081 tcggcgcgat tttcagacgg ccttcgacgt ttttcacgcc tgcagcgttt aactcgtcga
     4141 tttcgcccaa cagggcttcg ggggagacga caacgcccga accgatgaag cagtccagac
     4201 tttcatgcag gatgccgctc ggaatcaggc gcaaaatggt ttttttgccg ccgacgacca
     4261 aagtatgacc cgcattgtgg ccgccttgga agcgcaccac gccgccggct tcttccgcca
     4321 gccagtcaac gattttacct ttaccctcgt cgccccactg tgcgccgatt actacaacat
     4381 ttttagccat agccatataa cctatcgata ttaaaaatta ttgcggaaat ccccaaaccg
     4441 gtgcggcttt gcgccccacc acgatttgcc tttcatccgc catgaacggg tatttagcgt
     4501 ttcacgacct gccaaacgcc gtccgtcttt ttcagacggc ctgcaagctc ttccgaaaca
     4561 ttgtgtccga taccgtagtc aatcacgaca cactgccctt gttcacgcaa ggcttcgact
     4621 gcttcgcgcg ccgcttcggc atcttccgca tcgaccaaca cggcgggctg ccgttcgctg
     4681 gtgggcaaac gcccgataaa gctgcgcaag tcgaaactga atcccgttgc cgggcgcgcc
     4741 ctgccgaaat atccgcccaa tccgtcataa cgcccgccgc gcgcgaccgc gtcgtggaaa
     4801 tcggcggcat aggcggcata cagcaagccc gtgtggtaat tgtcgacgcg cagctcggac
     4861 aagtcgatat ggatttcaca atcggggaat gcgtcgcaca ccgcctgcaa ttcgcccaac
     4921 gcgccgccga ccgccgacaa atccggcaac cgtccgcgcg cgtcggacaa cacttcacgc
     4981 ccgccgtaca ggcgcggcag cagcgagaat gcttttgccc acataccgtc cagcttccaa
     5041 gccttgacct gcgcttcgac cgtctcggta tctttatcct gcatcaaggc aagcagcgtt
     5101 gcagactgcc ccgcatccaa atgcgccgca tcggacaagg cgcgaaatat gccgatatgc
     5161 cccagcgaaa gcagcacttt gcccatatcg gcaattttca tgcttttcag catcaggtct
     5221 atcagctcga tgtcgccacg gatgtcggca aaaccgtaca tttccgcccc tgcctgcaag
     5281 ggttcgcgca tattcagcag accgtcggac tgcgcgtgca acaccggacc ggcataacac
     5341 aaccggttaa tcccttggtt ggcggataaa agatgggcat cgatacgcgc cacctgcggc
     5401 gtgatgtcgg cgcgtatgcc caactgcctg ccgctgagcc tgtccgttac caaaatggtt
     5461 ttcagggaaa gccccgcatc gatatgcgtc agcagggaat gtgcgtactc catcagcgga
     5521 ggctgtacca gttcataacc gtgtacgcgg aacagtgcca acaactgctc cctcgcgctt
     5581 tcaagctgcc gcgcgttcgt gggcagtacg tcggcgatat gttccggaag ctgccatgtc
     5641 tgcatgagaa tctgccttct attttatcat gtcaatacaa aaggctgccc gatccgcaat
     5701 cagacggcat ctttgccgca acgcatcacg ccgctgtcaa aacggcgatg cttccttccg
     5761 attcagaatc cgaacagccc ctgtccgacg gctcaatgcc gtcaagtcgt taaaaccaaa
     5821 ctttaccata aaatacacac aatctgaata tacttttata ccctgtatcc gccgtctgac
     5881 ggcacggcat ttttttacga tataatgtgc gccattattt ctcatcactt cctgctgccg
     5941 ttttctgctt cagacggcat ttttgttgat tgaacactta tgctgctcga cctcaaccgc
     6001 ttttcctttt ccgtcttcct gaaagaagtc cgcctgctga ccgctcttgc cctgcccatg
     6061 ctgttggcgc aggtcgcgca ggtgggcatc ggttttgtcg ataccgtgat ggcgggcggt
     6121 gcgggcaagg aagacttggc ggcggtggct ttgggcagca gcgcgtttgc cacggtttat
     6181 attaccttta tgggcattat ggcggcgctg aacccgatga ttgcccagct ttacggcgcg
     6241 ggtaaaaccg acgaagtggg cgaaacggga cggcagggga tttggttcgg gctgtttttg
     6301 ggcgtgttcg gcatggtctt gatgtgggcg gcgattacgc cgttccgcaa ctggctgacc
     6361 ttgagcgatt atgtggaagg cacaatggcg cagtatatgc tgttcaccag cttggcgatg
     6421 ccggcggcaa tggtacaccg cgcactgcac gcctacgcct ccagcctgaa ccgcccgcgc
     6481 ctgattatgt tggtcagctt tgcggcgttt gtgttgaacg tgccgctgaa ctatattttc
     6541 gtttacggca aattcggtat gcccgctttg ggcggcgcag gctgcggact ggcgacgatg
     6601 gcggtgtttt ggttcagcgc gctggcattg tggatttata tcgccaagga aaatttcttc
     6661 cgcccattcg gactgacggc gaaattcggc aaaccggatt gggcggtgtt caaacagatt
     6721 tggaaaatcg gcgcacccat cgggctgtct tattttttgg aagccagcgc gttttcgttt
     6781 atcgtgtttt tgattgcgcc tttcggcgag gattatgtgg cggcgcagca ggtcggcatc
     6841 agtttgtcgg ggattctcta tatgattccg caaagcgtcg gctcggcggg gacggtgcgc
     6901 atcggctttt cgcttgggcg gcgcgaattt tcgcgggcgc gttatatttc gggcgtgtca
     6961 ctggtgtcag gatggatgct cgccgtgatt accgtgcttt ccttggtatt attccgttcg
     7021 ccgctggtaa gtatgtacaa caatgatccg gcggttttaa gcatcgccgc caccgtctta
     7081 ctgttcgccg gcttgttcca accggcagac ttcacccaat gtatcgcctc ctacgccttg
     7141 cgcggctaca aagttacaaa ggtgccgatg ttcatccacg ccgccgcctt ttggggctgc
     7201 ggtctgctgc cgggctacct gctcgcctac cgtttcgata tgggcattta cggcttctgg
     7261 acggcattga ttgcctcgct caccatcgcc gccatcgcct tggtgtggtg cttggaattg
     7321 tgcagtaggg agatggtcag atcgcataag gctgtctgaa aactccgccg attaaaaata
     7381 tagtggatta acaaaaacca gtacggcgtt acctcgcctt gccgtactat ttgtactgtc
     7441 tgcggcttcg ttgccttgtc ctgatttttg ttaatccact atagaaacga tgggctgaag
     7501 cccgccactg gcaatactca ttttaaagtt caaaccatgc aacccatccg ataccgaacc
     7561 gaccttaccc cctacaacac tttcggcctg cgcgcccaag cccgagcctt tattgcgctt
     7621 gaacacgccg acgggttgcg cgacatcgtc cggctacccg agttcaaccg agatactgtt
     7681 ttatggctgg gcggcggcag caatatcctt ttgatggagg actacgccgg tctggtcgta
     7741 catatggaaa acaaaggcat acgcgaaatt gctcgttcag acggcatggt tctgattgaa
     7801 gcgcaggcgg gcgaaatctg gcacgatttt gtcctgcacg ccgtcgcgct gggtttgaac
     7861 ggtttggaaa acctgagcct gattccgggt acggtcggcg cgtcgcccgt gcagaacatc
     7921 ggcgcatacg gcgtagaggc gaaagatgtg attcacagcg tgcgctgctt cgatttggat
     7981 acggaaacct ttgtaaccct ttccaatgcc gactgccgct tcgcctaccg cgaaagcctg
     8041 ttcaagcagg aaggtaaagg gcgttatgtg attgtttcgg tcgtatttgc attaaaaacg
     8101 cattttgtgc cgaacttggg ttacggcgat ttggcggccg ccgttgccga actgagcgcg
     8161 ggcagggtgc cgacggcgaa agatgtttcc gatgcagtgt gtgcaatccg caacagtaaa
     8221 cttcctaatc ctaacgtgct tggcaatgtc ggcagtttct ttaaaaaccc cgtcgtcagc
     8281 gcagaaaaag ccgccacctt gttgcagcgg catcctgata tgccgcgcta tccgcagccc
     8341 gacggttcgg tcaaactcgc cgccggctgg ctgatcgacc aatgtcgtct gaaaggcttt
     8401 caaatcggcg gcgcggcggt acatgacagg caggctttgg tcttagtgaa caaaaacaat
     8461 gcctcggcaa acgatgtccg gcagttggcg caacacatca aatttacagt atttgctcgg
     8521 tttcaggtag aattgcacgc cgaacccaat tggctgcctg cttcgttcag cctgtaaatc
     8581 caaggagtat gcccgtgacc cgccccgcca aaatcaatac ttacacacgc atcatcgacg
     8641 ccagccttgc gcttttcaac gaggaaggcg agcgcaacat cagcaccaac catattgccg
     8701 cccacttggg catcagtccg ggcaacctct attaccactt ccgcaacaaa gacgaaatca
     8761 tcgtccaact gttcaaacgt tacagcgaag ccctgctgga atacctcaac gaagccgtgt
     8821 tgccgtctga tgtggaagac tccatcaatt atatggcggg catttacgac gtgatgtggg
     8881 aataccgctt cctattcagc gacgtgaaca ccctgcttgc acgcagtgcc gaactattgg
     8941 gcgaacacaa taccttcacc caagccaaag tctccccgct cttggtcaac ctgctgaccc
     9001 agctcaacgg tctgaacatc atccaagccg accaaaccgc catgaacgac ctcgccgtca
     9061 atatgtggat gatcacgaaa tactggttcg acttcgacag ctccctgcgc ggccgcacca
     9121 agctgaccga agactccaaa gcacgcggcg tccgtcgtac cttaagcctc cttcgtcctt
     9181 atcttttgcc cgaacaccgc gagaaatacg accggaaaat cggcaacggc aacccgtaaa
     9241 cccacagcgt ccgaatcagc cttcagacgg cacttacccg acggtatcag cgaatgcgcg
     9301 cagtcccccc gccccatatc tccatcacag gcctaggtta cctcggcctg ccgctggcac
     9361 aaaagtttta ccaacacggc agccgcgtcg ccgccataaa acgcagcctg acttcggacg
     9421 acatcaacct gcccatacat ctcgacacct tcgacctcgg cagcaccgat gcctttcaga
     9481 cggcattgtg gcggcaccac gccgacaaac ccgtctggtt cttcctgcta ccgccctcct
     9541 cgctgacgca ttacgccgac accgtcaaac aatgggcaga acttgcccgg gcgtgcaacg
     9601 tgcaacacct gattttcaca agcagcacca gcgtttacgg cgatacagcg cgcgaatgcg
     9661 acgaaaccgc cacacccgat ccgcaaaccg agtccgcccg ccaaatcctc gccgccgaac
     9721 aacacctgct cgacagcggc atctccaaca tcgacatcct gcggctgggc gggctttatt
     9781 gcgccgaacg ccatcccgtc agccgccttg ttcaaaagca aaacatcccg ggcggcaacc
     9841 gccccatcaa catcgtccac cgtaatatcg ccgtcgaaag cctgtttcag acggcattta
     9901 accccggcgg caggcggctg aaaaacatta tcgaaccgcg ccacccgaca cgacgcgaat
     9961 tctacacgga agaagccgcc aaactcggcc tgcccgcgct ggatttcgca cccgacgaca
    10021 gcgtgggcaa aatcatccgt accgtttgcg ataacggctt aagcctgtaa aataagcggc
    10081 aacagcaaca aacaggcatt ctccgcccca atcagaaaat atgtccgcct tcctccccat
    10141 catcaaccgc ctgattctgc aaagcccgga cagccgctcg gaacttgccg cctttgcagg
    10201 caaaacactg accctgaaca ttgccgggct gaaactggcg ggacgcatca cggaagacgg
    10261 tttgctctcg gcgggaaacg gctttgcaga caccgaaatt accttccgca acagcgcggt
    10321 acagaaaatc ctccaaggcg gcgaacccgg ggcgggcgac atcgggctcg aaggcgacct
    10381 catcctcggc atcgcggtac tgtccctgct cggcagcctg cgttcccgcg catcggacga
    10441 attggcacgg attttcggca cgcaggcaga catcggcagc cgtgccgccg acatcggaca
    10501 cggcatcaaa caaatcggca ggaacatcgc cgaacaaatc ggcggatttt cccgcgaatc
    10561 cgagtccgca aacatcggca acgaagccct tgccgactgc ctcgacgaaa taagcagact
    10621 gcgcgacggc gtggaacgcc tcaacgaacg cctcgaccgg ctcgaacgcg acatttggat
    10681 agactaacct tcagacggca tccgacatga acagcccttt tcacaacatc ggcatcgtaa
    10741 cccgccccaa cacgcccgac atccaagaca ccgcacacac gctgattacc tttttgaagc
    10801 agcacggctt taccgtctat ctcgacgaag tcggcataaa ggaaggctgc atctataccc
    10861 aagacaccgt cggctgccat atcgtcaaca agaccgaact ggggcaatac tgcgacctgg
    10921 tcgccgtttt aggcggagac ggcacctttc tctccgtcgc ccgcgaaatc gccctgcgcg
    10981 ccgttccgat tatcggcatc aaccaagggc atttgggctt cctgacccaa attccccgcg
    11041 aatatatgac ggacaagcta ttgcccgttt tagaagggaa ataccttgcc gaagagcgca
    11101 tcctgattga ggccgcactc atccgcgaaa gcaaaaccgc cgaacgcgcc atcgccctca
    11161 acgatgccgt cctctcccgt ggcggtgccg gacagatgat tgagtttgaa gtcttcgtca
    11221 atcgggaatt cgtctatacc cagcgttcgg acgggctgat tgtctccacc cccaccggat
    11281 cgaccgccta ttcgcttgcc gccggcggcc ccatcatgca ggcaggatta cacgccttca
    11341 cgctcgtccc catctgcccg caatccatga ccaaccgccc catcgccatt ccagacacgt
    11401 ccgaaatcga aatcctcgtt acccaaggcg gcgacgcgcg cgtccatttc gacggtcaaa
    11461 cccatatcga cgtgcaaaac ctcgaccgca tcaccatccg ccgctaccga aaccccttac
    11521 gcatcctaca tccgaccgac taccaatatt tcaaaaccct gcgccaaaaa ctgcactggg
    11581 gcgaacaatt ggtctgaacc gacccttacc ggaaaacacc tatgaatcca aaaactcccc
    11641 ccgaccatac agaagatgcc gtgcgcctct ccaaacgcat ggcgcaactg gggctttgtt
    11701 cgcgccgcga agccgacggc tacatcgaac agggctgggt aacggtcaac ggcaaaaccg
    11761 ccgtacttgg tcagaaagtt tcaccagcag accgtatcga actgaacaag aaagcccacg
    11821 aacagcaggc ggcacgcatt accatcctgt tgaacaaacc cgtcggctat gtcagcgcac
    11881 aagcggaaaa aggctataaa tccgccgccg aactgattac ccctgaaaat cactgggaag
    11941 gcgataccag ccgcatccgt ttcgatccga aacacaaaat cggtcttgcc cccgccggca
    12001 gactcgatat cgattcagtc ggattgctgg tgttgacgca ggacggccgt atcgccaagc
    12061 agcttatcgg cgaaaacagc ggcagtgaaa aagaatattt ggtgcgcgtg cgcggaaaat
    12121 tggacgaaaa aggacttgcc ttactgaatc acggattgag tttggacggc gagaaactgc
    12181 gtcccgccca agtagaatgg caaaacgaag accaactgcg cttcgtgttg aaacagggta
    12241 aaaagcggca aatccgccgt atgtgcgaac tggtcgggct gcgcgtcgtc gggctgaaac
    12301 gcatccgcat gggcaaggtc aaactcggca ggctgccgcc cggcaaatgg cgttatctcg
    12361 cccccggcga atcgttttaa ataagcatta aaaaatgccg tctgaaaatc cttgcgtttt
    12421 cagacggcat tgccttatgc ccaaataacg gtttcttcca agggcctgcg cgccttcgtg
    12481 gcgatttcct gaacgcggta gccgaatgtc gcggcgacgg acacgcccca ttctgccgca
    12541 tcgaacaaac cgaactgcct ggacaatacg cgttccatat cggcatagtt gaaaccttcc
    12601 accggacagg aatcgatacc tgccatcgcc gcacccgtca tcatgttgcc caacgcgata
    12661 taggtctggc ggcaacacca gtcaaacaag gcgcgcgaat cgtccaaaat cttgatgtcg
    12721 tcggcttgaa acgcccgata cctcgccaaa gattttgcca tggcatccgg ttcggtaacg
    12781 ccgcgccgtt tgaggctttc caacataaac gggctgtcga agcgggcatt tttctttgcc
    12841 aaaaacacca ccaaatgact ggcggtatcc aaagcatccg ccatacccca agaaaacggc
    12901 ttgattgcct gtcggatttc ggggttttga accacaacaa actgccaagg ctccgaaccg
    12961 accgaactgg gcgacaaacg cccgagttct aaaataaact gaaaatcctc ggcactgatt
    13021 ttacgtgccg catcgtaatg ccggcatgat ttgcggtttt taaatgcgga taggacctgc
    13081 tctttgctta atactgtcat cgtgcatcct tttattactt tgattgcaaa ttgatttcaa
    13141 accgatacgg ctctgccgca ccatacaatc cgtcctcgcc atcaagcttc atccggactt
    13201 tacccatctc tgctacggct gggaaaacaa agcggcggca gactcgggaa taacgcccca
    13261 atgcctttca gacggcattt gggcatcatt gcaaacgcgc ctacgggtag gcgatatagg
    13321 cataagccga atggttgtgg atagactcga aattctcgct ctcgacaacg aaactcttga
    13381 tgcgtttgtc ggcgatcagc gaagtagcga catcgcgcac catatcttcc acgaatttcg
    13441 ggttttcgta ggccttttcg gtaacgtatt tttcatcggg gcgtttgagc aggccgtaga
    13501 gttggcagct cgcctgcgcc tccacataat cgatgacttc ctcgataccg acttcggcat
    13561 cggcagtcag gctgacggta acgtgcgaac gctggttgtg cgcgccatat tgggaaattt
    13621 ctttggaaca cgggcaaagc gaggttacgg gaatcatgac cttcatactg tggccgtatg
    13681 ccccgtcttt gatttcgccc gtgaggctga catcataatc cagtaaggac cgaataccgg
    13741 aaaccggggc ggttttcttg cggaaaaatg ggaaagaaac gctgattttg ccggcgcggg
    13801 aatccaaaag cgcgaccatt tcagcagtca gcctgcgcaa ttgtgcaaaa tccaaggctt
    13861 cggtatgttg ctccatcaat gcgacaaagc gcgacatatg cgtacccttc tgctcggcag
    13921 gcaggtaaac cgtcatggtc agacgggcaa ctgtggactg gatgccttct gcagtttgca
    13981 gggtaatcgg aaagcgcagg tctttgatac cgacctgatt gatcggcaga ttgcgcaaat
    14041 ctcggctgga ttgcacgtct gcaatagtgt tcatgagttg tttttcctta atattcatgt
    14101 aattgttgag atgtgtgcat ggtatggcgt ttccaccggg gaatataccg tcaatctgca
    14161 aatgcgagat tatatcactc gcttttagca gctgcattga ttgtttctat ttttcagata
    14221 gtgataaaat cttcgattat tcacatctac ataggcagag accaaatgaa attcgccacg
    14281 aaagccattc attccagcta cgattgcgac gaacacaacc gcgcgctgat gccgccgatt
    14341 tatcaaaaca gtatgttcgc gttgcacgag attggcgaaa atgtgcctta ccgctactcg
    14401 cgcctgagca acccgacccg tcagatttta gaagacaccg ttgccgattt ggaacacggt
    14461 gcggcaggtt ttgcgttttc cagcggtatg gcggggattg atgccgtgtg gcgtaccttc
    14521 ctacaaccgg gcgataccat cgtcgcggtc gccgatattt acggcggcgc gtatgattta
    14581 ttggtcgatg tttatcaaaa atggggggtg aacgttgttt ttgccgattt gggcaatccg
    14641 gataatttgg acgaattgct caaagcgcac aaggtcaaac tggtttggct ggaaacgccg
    14701 tccaatccgc ttttgcgctt ggtagacatc aaagcgcttg ccgcgaaagc caaagcggcc
    14761 ggcgcactgg tcggtatcga caacaccttt gccacgccgt atctgcaaca gcccttggat
    14821 atgggttgcg attttgtgtt ccattccgct accaaatatt tgtgcggcca ttccgacgtg
    14881 ttgatgggca tcgtcgttgc caaaaccaaa gagctggcgc agcctttgca cgatatgatg
    14941 gtgcataccg gcgcggttgc cggcccgatg gactgctggc tggtgttgcg cggcatcaaa
    15001 acactggctc tgcgcatgaa cgcccattac caaaacgcac tcgaaatcgc gcgccgtttg
    15061 gaagcccatc ctgccattga aaaagtgttc catcccggcc tgccgtctca cgaacattac
    15121 gaactggcga aaacacaaat gcccaaaggc ataggcggcg tggttacggt ttatctcaaa
    15181 aacgacacgc gtgaagcggc aaacagcgtg attaaaaaca tgaaactggt caaaatggct
    15241 tccagcctcg gcggcgtgga aagtttggtc aaccattgct attcccagtc ccacagcggc
    15301 gtgccgcata atgtgaaaat ggaaatgggc atcagggtcg gcctgctgcg tttctctgtc
    15361 ggtatcgaag atgtagatga tatttggaat gatatttccg ccgcactgga tacgactttg
    15421 taaaccgtaa aaatgccgtc tgaaaccatg gtttcagacg gcatttcaat taacccggcc
    15481 gaaaatcaac gcttcagcat cttggccgcc tcaatggcat aataggtcaa aatcccatcc
    15541 gcgcccgcac gtttgaatgc cagcaggctt tccaaaacca ctttatcgcc atccagccat
    15601 ccgttggcaa ccgccgcctg tagcatcgca tattcgcccg aaacctgata ggcataagtc
    15661 ggcacaccga actcgtcctt cacgcggcgg acaacgtcca aatacggcaa accaggcttg
    15721 accattacca tatccgcacc ttcctgaatg tccaacgcca cttcgtgcaa cgcctcatcg
    15781 gtatttgccg gatccatctg gtaggttttc ttgtccgcct tgcccaaatt gcccgaactg
    15841 cccaccgcat cacggaaagg gccgtaaaat gcagaagcat atttggcgga atacgccata
    15901 atcagcgtat ggatatgccc ggcatcctcc aacgcctcgc gaatcgcacc gatacgcccg
    15961 tccatcatat cggaaggagc aaccacctgt gcgcccgctt cagcgtggca caaagcctgc
    16021 ttgaccaaaa cctctacggt ttcatcgttc atcacataac cgttttcgtc cgtcagcccg
    16081 tcctgaccgt gaaccgtata aggatcgagc gcgacatccg tcataatgcc cagttcagga
    16141 aacctctcgc gcaaggctcg gacagttgac ggcacgagtc cttcgggatt gtacgcctcc
    16201 tgcgcacgct cagttttgtt tgccgtaacc acgggaaaga gtgccaacat cggaataccg
    16261 agctttaccg cctcttccgc cgtaaacagc agcctgtcca aactctgacg cttcacgccc
    16321 tgcatagaag gcacatcctc ctcgcgcgcc gacccctcca atacgaacac cggataaatc
    16381 aaatcatcgg cggtcagcgt gtgttcgcgc atcaggcggc gtgaaaaatc gtccctgcgc
    16441 atacggcgca tacgcgaagc cggaacattg cggtaaggaa actgcataag ccctccaatc
    16501 atcctgtcaa caattcaaac catacggaag ccgccgcaac ttgcagacag catcgggcgg
    16561 caatcaaaat ccgttttcac cggaaagggt ttcggcccgc cgaaatgccg caccgaagga
    16621 cggcgtcccg cccattaaag aacaatagca aaacaacagc ttcaggctgt cagccttcga
    16681 tattcttacg ccacaatacc aaaagtttcc cgatatgctg aacaggttgc gcgtcaaccg
    16741 cctcacataa ggcagtgcag atttcgatac gttcggcacg gtcgtcgccg aatacgcgca
    16801 ctttaatcag ctcatgcgcc gtcaatgccg catcggtttc cttgatgacc gcgtccgtca
    16861 gaccctgctg accgaccatc acaacaggat ggagatggtg cgcgcgcgct ttcagttcca
    16921 aaatttcttt ggtgttcaat ttggtatccg tcattttcct actgcttgaa taaaaagaat
    16981 aatgcggcat tgtacgcgat ttggcacgac atcggcaaaa ttaacgtaca atacgcggtt
    17041 tccattccga cccgcgaacc actatggctg tacgttccaa atcctcaaaa gcgtggctgc
    17101 acgaacacgt caacgaccac tacgtccata tggcgcaaaa agacggctac cgcgcccgtg
    17161 ccgcatacaa acttttggaa atcaacgaaa aagacaaatt aatcaaaccc ggcacggtac
    17221 ttgccgactt gggcagcgca ccgggaagct ggtcgcaggt tgccgccaag ctgacgggta
    17281 cttccggagc agttttcgcc ttggacatcc tgcctatgga agccataggg ggcgtctcct
    17341 tcattcaggg cgacttccgc gagaacgacg tactggcaca attcgaaacc ttgttggaca
    17401 accgcccgct cgaccttgta atttgcgata tggcgcccaa tatgtcggga aacgccgtaa
    17461 gcgatcaggc acgcagcttt tatctgtgcg aactggcttt agacttcgcc tcgcaacacc
    17521 tgaaaaccgg cggcagcttt ttggtcaaag tctttcaggg tgcaggctat caggaataca
    17581 tggcagccat gcgcgaaatt ttcggcacgg tgcagacgcg caaacccgaa gcctcgcgca
    17641 atcgctccag tgagatttat ttattgggca aaaataaacg ctgacaatac agacggcgtg
    17701 ctttacaatc atttccgttt tacacctcaa ttatggagcc ttgctaagtg gggaacacct
    17761 ttaaatcaat ccttgtctgg gtcgccttgg gtatcggcct gatggctgcg ttcaacgctt
    17821 tagacggtaa aaaagaagac aacgggcaaa tcgaatattc tcagttcatc caacaggtca
    17881 acaacggcga agtatccggc gtcaacatcg aaggatccgt cgtcagcggc tacctgatta
    17941 agggcgagcg caccgacaaa agcaccttct tcaccaacgc gcctttggac gacaacctga
    18001 ttaaaacact gctcgacaaa aacgtccgtg taaaagtaac gccggaagaa aaaccgagcg
    18061 cgctggctgc cctgttttac agcctgctgc ccgtcctgct gctgattggc gcgtggttct
    18121 actttatgcg tatgcagacg ggcggcggcg gaaaaggcgg cgcattctca ttcggcaaaa
    18181 gccgcgcacg cctgctggac aaagatgcca acaaagtgac ctttgccgat gtcgccggct
    18241 gcgacgaagc caaagaagaa gtgcaggaaa tcgtcgatta cctcaaagca ccgaaccgct
    18301 atcaaagctt gggcgggcgc gtgccgcgcg gcatcctgct ggcgggcagc ccgggtacgg
    18361 gtaagacgct tttggcaaaa gccattgcag gcgaagccgg cgtgccgttc ttcagcattt
    18421 caggttccga ctttgtcgaa atgttcgtcg gtgtcggtgc gagccgcgtc cgcgatatgt
    18481 tcgagcaggc gaagaaaaac gccccctgca tcatctttat cgacgagatt gacgcagtcg
    18541 gccgccaacg cggcgcaggt ttgggcggcg gcaatgatga gcgcgagcaa acattaaacc
    18601 aattgttggt tgaaatggac ggttttgaga gcaatcagac tgtaattgtg attgcggcaa
    18661 ccaaccgccc cgacgtactc gatcctgcgc tgcaacgccc cggccgtttc gaccgccaag
    18721 tcgtcgtccc cctgccggac atccgggggc gcgaacagat tttgaacgtc cattctaaaa
    18781 aagtgccttt ggacgaatct gtggatttat tgtccctcgc gcgcggcacg ccgggttttt
    18841 ccggtgcgga tttggcgaac ttggtcaacg aagccgccct gtttgccggc cgccgcaata
    18901 aagtcaaagt cgatcagagc gattttgaag acgccaaaga caaaatctat atgggtccgg
    18961 aacgccgcag tatggtgatg cacgaagacg aaaaacgtgc gacggcgtat cacgaatccg
    19021 gacacgcgat tgttgccgaa agcctgccct ttaccgaccc cgtccacaaa gtaaccatta
    19081 tgccgcgcgg acgtgcgctg ggtctgactt ggcagcttcc ggagcgcgac cgcatcagta
    19141 tgtataaaga tcagatgttg agccagctct ccatcctgtt cggcggacgg attgccgaag
    19201 acatcttcgt cggacgcatc tccaccggcg catcaaacga ctttgaacgc gcaacccaaa
    19261 tggcgcgcga gatggtaacg cgctacggca tgagcgacaa aatgggcgtg atggtttatg
    19321 cggaaaacga aggcgaagtc ttcttgggac gcagcgtaac ccgttcgcaa aacatttccg
    19381 agaaaaccca gcaggacatc gacgcggaaa tccgccggat tttggacgag cagtatcaga
    19441 ttgcctacaa aatcctcgat gaaaaccgcg acaagatgga aacgatgtgc aaagccctga
    19501 tggaatggga aaccatcgac cgcgatcagg tactggaaat catggcgggc aaacaaccca
    19561 gcccgcccaa agattacagc cacaacctgc gcgagaatgc ggacgcggcg gaagataacg
    19621 cgccgcacgc tccgactcgg gaagaaaccg aagcacctgc cccggcagac accgcttcga
    19681 cagagtccga gcagcagcct gaaaacaagg cttaacttcc cgaacaaacg gcagcccgca
    19741 agctgccgtt ttcccatccc gttttcagac ggcattttcc actcaatgcc gtctgaaacg
    19801 ccaaagccgt atcggttttt acgaatctgc gatatggcgg tttaaattga aatcagaacg
    19861 gggcaaggct gtaccggttt aaaattaaac cgctataata ccgcccttcg aatacacgcc
    19921 cgcattggtg atgcccgtga aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga
    19981 caaaatgttc gagctggtcg acaaggttga ggattacccg cactttctgc cgtggtacag
    20041 caagaccgaa gtcatcgggc gtagcggcaa cgaactgaag gcgcggctgt ttatggatta
    20101 tatgcacgtt cgccaatcgt ttgccacgca caaccgcaac attccgggca gggaaatccg
    20161 tatggaactg ctcgaaggtc cgttcaaaac cttacgtgga acgtggaaat ttatcgattt
    20221 gggcgacgat atgtgcaaaa tcgaattcaa tttggaatac gatttttcca atgccgtttt
    20281 gtccgcctta atttcccccg tcttcaacca cctttccgcc acgctggtcg aagcgttcgt
    20341 caaagaggca gaccgccgtt atgcttgaaa ttgagattgt gtacgggctg cccgaccgac
    20401 aggttttgaa aaccatgcag cttgccgagg gaacaaccgt ccgcgccgcc gcactgcaaa
    20461 gcggtttgga cggcatattt gaagatttaa acctgcattc cgcgcctttg ggcattttcg
    20521 gcaaagccgt caaagacgac acgcccctgc gcgacggcga ccgcatcgaa gtgtaccgcc
    20581 cgctgttgat cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac
    20641 catgccgtct gaagccttca gacagcattc gacagaaaca cggaaaatat catgtcaaac
    20701 aaaatcaaaa tgcttgtcgg attgggcaac cccggcaaag aatacgaaca aacccgacac
    20761 aatgcgggtt tttggttcct cgacgaactg gcgtggaaat ggaaggcttc atttaaagaa
    20821 gaaaaaaaat tcttcggcga agtcgcccgt gccgccctgc ccgacggcga cgtttggctg
    20881 ctcaaacctg ccacgttcat gaaccgttcc ggacaggcag ttgccgcgct tgcacagttc
    20941 tacaaaatca aacccgaaga aatcctcgtc gtccacgacg aactcgacat tccctgcgga
    21001 cggattaaat tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag
    21061 gcaaaactcg gcacggcaga ctattaccgc ctgcgcctcg gtatcgacca cccgggcgac
    21121 cgcaacctcg tcgtcggcta tgtcctgaac aaacccagta cggaacaccg ccgacagatt
    21181 gacgatgccg tcgccaaatc cctgcaagcc atacccgaca tccttgccgg caaatgggaa
    21241 gaagcaaccc gcttcctgca cagcaaatga cccgatgccg tctgaagccc tttcagacgg
    21301 catgttcccg atttccatat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc
    21361 gaatccatcc ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac
    21421 gacgcaccat ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc
    21481 aaattcctgc gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc
    21541 tgccgcgcaa ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc
    21601 tgctcttcaa agctgccgac accgcgccgc cgccgtttcc gcatttcgac aaagcagcac
    21661 accttgccct gtttttcgca caaatctggc ttttgaccaa agcattcaaa accggaaaac
    21721 ttcccatccc ctaccgcagc ctgatggtct ttgccctctg tttcgccctc ttcagcgaat
    21781 gcgcgcaggc atggtttacc gcaacgagaa ccggcagttt gggcgatgtt cttgccgata
    21841 tggcaggtac ggttctcgca ctctttgccg cccgcgccgc cgaccgcccg gactgaatcg
    21901 gtttatttcc cccaaacagg atgcacctct tcccgataca tcctattttt ccccttacat
    21961 atcaatccca tctcataaac aaaaccgaca aatcgtttac aatatattta cactacatca
    22021 gattacaaat atactcgaac cagttgtaga atcggcggtt ccacaaaccg tttcggattg
    22081 cacgacacaa aagaaaaacc gattttattg ccttaaagga gtattcatga acctgcatgc
    22141 aaaggacaaa acccagcatc ccgaaaacgt cgagctgctc agtgcgcaga agccgattac
    22201 cgactttaag ggcctgctga ccaccattat ttccgccgtc gtctgtttcg gcatttacca
    22261 catcctgcct tacagccccg atgccaataa aggtatcgcg ctgctgattt tcgttgccgc
    22321 actttggttt accgaagccg tccacattac cgtaaccgca ctgatggtgc cgattctcgc
    22381 cgtcgtactc ggtttccccg acatggacat caaaaaggcg atggctgatt tttccaaccc
    22441 gattatctac atttttttcg gaggcttcgc gcttgccacc gccctgcata tgcagcggct
    22501 ggaccgtaaa atcgccgtca gcctgttgcg cctgtcgcgc ggcaatatga aagtggcggt
    22561 tttgatgttg ttcctcgtta ccgcctttct gtccatgtgg atcagcaaca ccgccaccgc
    22621 cgcgatgatg ctgcctctag caatgggtat gctgagccac ctcgaccagg aaaaagaaca
    22681 caaaacctac gtcttcctcc tgctcggcat cgcctattgc gccagcatcg gcggcttggg
    22741 cacgctcgtc ggctcgccgc ccaacctgat tgccgccaaa gccctaaatc tggacttcgt
    22801 cggctggatg aagctcggcc tgccgatgat gctgttgatt ctgcccttga tgctgctctc
    22861 cctgtacgtc atcctcaaac ctaatttgaa cgaacgcgtg gaaatcaaag ccgaatccat
    22921 cccttggacg ctgcaccgcg tgatcgcgct gttgattttc cttgccacag ccgccgcgtg
    22981 gatattcagc tccaaaatca aaaccgcctt cggcatttcc aatcccgaca ccgttatcgc
    23041 cctgagtgcc gccgtcgccg tcgtcgtctt cggcgtggcg caatggaagg aagtcgcccg
    23101 caataccgac tggggcgtgt tgatgctctt cggcggcggc atcagcctga gcacgctgtt
    23161 gaaaacatcc ggcgcgtccg aagccttggg acagcaggtt gccgccacct tttccggcgc
    23221 gcccgcattt ttggtgatac tcatcgtcgc cgccttcatt atttttctga ccgagttcac
    23281 cagcaacacc gcctccgccg cattgcttgt accgattttc tccggcatcg ctatgcagat
    23341 ggggctgccc gaacaagtct tggtattcgt catcggcatc ggcgcatctt gtgccttcat
    23401 gctgccggtt gccacaccgc ctaacgcgat tgtgttcggc acgggcttaa tcaagcaacg
    23461 cgaaatgatg aatgtcggca tactgctgaa catcctctgc gtagtattgg ttgctctgtg
    23521 ggcttatgct gtactgatgt aaaccatcga cctaaacaac aagaccgtct gaaagaatat
    23581 ttttcagacg accttgaagt ttgtcgttca gacacaattt gtcgaatcat tcaaaaccgg
    23641 attctaacga aaggaaaccc atgattatcc tgcacaccaa caaaggcgac atcaaaatcg
    23701 aactcgattt cgacaaagcc cctgttaccg ccaaaaactt cgagcaatac gtcaaagacg
    23761 gcttctacga cggcgtaatc ttccaccgcg tcatcaaagg cttcatgatt caaggcggcg
    23821 gcatggatga aaacatgaac gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca
    23881 acggcctgcc caacgataaa tacaccatcg ccatggcacg cacttccgac ccccattccg
    23941 ccagcgcgca attcttcatc aacactgccg acaacgcttt cctgaacttc cgttctaaag
    24001 agctgtacgg caaaaccgtc gtccaagact ggggctatgc cgtattcggt aaagtcgttg
    24061 acggttttga tgttgtcgat gccatcgaag gcgtatccac caaacgccac ggctaccatg
    24121 acgacgtacc gagcgaacct gtcgtcatca ttaaagccga agcggtataa accggcaatc
    24181 cgaaagcagc ctgcacaaag gctgcttttt ttgtttcagg cggcataaaa tccaagcaca
    24241 tatgccgtct gaacacctcc cgcaagcctt cggacaatgt tgcaacgtgt taaaatagcc
    24301 tcctttcctg aaaccacaac aaggaaacca tcatggcaaa tattgcccgc gacatcttca
    24361 aagcctacga catccgaggc attgtcggca aaaccctgac cgacgatgcc gcctatctca
    24421 tcggcagggc catcgccgcc aaagccgccg aaaaaggcat gacccgcatc gcgctcggac
    24481 gcgacggacg cttgagcggt cccgaactga tggagcacat ccaacgcggc tttaccgaca
    24541 gcggcatcga cgtcctcaac gtcggtatgg tcgccacgcc catgctctat tttgccgcca
    24601 tcaacgaatg cggcggcagc ggcgtgatga ttaccggcag ccacaatccg cccgattaca
    24661 atggtttcaa aatgatgctc ggcggcgaca cgctcgcagg cgaagccatt caagaacttt
    24721 tagccattat tgaaaaagac ggttttgttg ccgccgacaa acaaggcaac gtaaccgaaa
    24781 aagacatctc cggcgaatac cacaaccaca tcgtcggcca catcaaactc aaacgcccga
    24841 tgaacatcgc tatcgacgcg ggcaacggcg tgggcggcgc gtttgccggc aaactctata
    24901 aaggcttggg caacgaagta accgagcttt tctgcgacgt ggacggcaat ttccccaacc
    24961 accatcccga cccatccaaa cccaaaaacc tgcaagattt gattgccgcg ctgaaaaacg
    25021 gcgatgccga aatcggcttg gcgtttgacg gcgatgccga ccgcttgggc gtagttacca
    25081 aagacggcaa catcatctat ccagaccgcc aactgatgct tttcgcgcaa gacgtgttga
    25141 gccgcaatcc taaagccaaa gtaattttcg acgtcaaatc cacccgtctg cttgcgcctt
    25201 ggattaaaga acacggcggc gaacctgtga tggaaaaaac cggccacagc tttatcaaat
    25261 cctccatgaa aaaaaccggc gcgccggttg ccggcgaaat gagcggacac atcttcttca
    25321 aagaacgctg gttcggcttc gacgacggtc tgtacgccgg cgcacgcctc ttggaaatcc
    25381 tgtctgcctc cgataatccg tccgaagtgt taaacaacct gccgcaaagc atttccacgc
    25441 ccgaactcaa catcgccctg cccgaaggca gcaacggcca tcaggttatc gacgaactcg
    25501 ccgccaaagc cgaatttgaa ggcgcaaccg aaatcatcac catcgacggt ctgcgcgttg
    25561 aatttcccga cggcttcggt ctgatgcgtg cttccaatac cacgccgatt ctggtgttgc
    25621 gttttgaggc ggatacgcaa gaagccatcg agcggattca aaaccaattc aaagccgtca
    25681 tcgaaagcaa tccgaatcta atctggcctc tgtaaacaca ggaaaaatgc ttaatcaaat
    25741 cttggtagat tggatttggc ttaaaaatct ccgcgtccgt tcgtgtttgg ggtggtcgaa
    25801 caactctttc gggctgccct gctctacgat aacgccgccg tccatcacga caacggtcgt
    25861 ggcaacttcc agcgcgaact tgatttcgtg ggtaacgacg accatcgtcc aaccttcccg
    25921 cgccaattcc ttcatggcgt tcaacacgtc ttgcaccaat tcagggtcga gtgcggaagt
    25981 gggttcgtca aacaacatca gctcgggctg aatcgccaat gcgcgggcaa tgccgacgcg
    26041 ctgctgctga ccgccggaaa gctggtaggg atacaaatcc actttgtcgc ccaagccgac
    26101 tttttccagc agtttcagag cctcttcgcg cgcttgggcg gcaggcttgc cctgtacggc
    26161 aaccggtcct tccattacgt tttccaaggc ggttttgtgc ggaaagaggt tgtattgttg
    26221 aaacaccatg cctgatttgc ggcgcagtgc caaaatatcg tgtttgcttg gttttttaga
    26281 aaaatcgatt ttcagcggtc gctcgttgtc gaactcgatt tgtccgtctt cgggcatttc
    26341 caacgcgttt aggcatcgca gaaacgtcgt tttgcctgag ccggaaggcc cgaggataac
    26401 gaccacctgc cctttgccca catccaaatc gatgccgcgc aaaatagtgt tttcgccgaa
    26461 ggtcttatgg atattgcgga ttttaatcat aacaactcct tatttggcga cataacggtc
    26521 gaaacgtttt tccaaacgtg cctgaatcag gaacagaacc ttgcagaaac accagtaaac
    26581 caatgcggct tcaatataga cgggcaaaaa gtcgtaagtg cggtttgccg tttcctgcgc
    26641 gacgcggaac aattcagtta ccgttaccac cgctgcgagc gaggtgtttt tgaacaagcc
    26701 gataaactcg ttgctcaaag gcggcacggc aacgcggaat gcctgcggcg cgacgatgcg
    26761 gcggaacgtc tgcatatagg tcataccgat ggagaaacct gcttcccatt ggcctttagg
    26821 tacggacaaa attgccgcgc gtatggtttc ggaagcgtat gcaccgacat tgagcgagaa
    26881 gccgataatg gcggcgggaa tcggatcgat atagataccg acggacggca gcccgtaaaa
    26941 cacaatcaca agctgcacca acagcggcgt accgcgaatg acggaaatat aaaattccac
    27001 caatttcagc aggattttcc gcgcaatgcc gccggcgggc ataatccgca ccaaagccac
    27061 ggctacggca ataaccatac cgataacgaa agaagctgcc gccaaaggca gagacaccgc
    27121 gaagccggct ttgaccatag gcaaaaacgc gctgacaatc atatcggcgc gtgtttccgt
    27181 cataaacggc agcgaagcaa ggaaattatt gaacactgat gtcttttccg aagaattgtt
    27241 cgcccagttt tttcagcgta ccgtcggctt tcagctcgtt gattgccgta ctgaatttcg
    27301 ccacggcttc gtcattgccc ttgttgacaa tcaggccgga accgactttt tcatcggcag
    27361 gtgcggacca aacgattttc acgcccgcat tcgggttttt cttcagatag tccaaaaccg
    27421 ccaattcgtc gttcagggtt gcatcggcac gtttttgttc aatcagggtc agcgattgcg
    27481 ccaaaccgtc cactgccacc aaatctgcgc ctgcagcttt ggctttttcg ccgtagttgc
    27541 tggtcaggga ttgtgcggtt ttcacgcctt tgatgtcgtc gatagatttg atgttgctgt
    27601 cgttacgggc aaccaatacg gcaccgctcc agctgtaagg atcggatttg tcgaatgtcg
    27661 cttggcgttc ggggctggtc agaccgactt ggtttgccac cacgtcgaaa cgccccgcct
    27721 tcaaacccgc catcatcgaa tcccattgcg tttctttaaa ctcgactttc acgcccagtt
    27781 tttccgccac ggcgcgggtt acttccacat cgtaaccggt cagtttgccg tccttgtcgt
    27841 ggtaggtaaa cggtgcgtaa gtgccttccg tgccgacggt aaccgtgcct ttattgttga
    27901 tgcgctcgat taaagaaccg gaaactgccg attgtgcagg cgcggaagat gctccgctgc
    27961 cgccttccga accgccgcag gccgccaaaa ccaatgcggc aataccgccg agtacgaatt
    28021 ttttcaacat catcctctcc tgtaaaaagt gtgcaagtgc ggcattctaa gtgaaattgc
    28081 ggcaaacaca aagaatcctt tttttatatg gttattccaa atcggaatgg aaaagatatg
    28141 ccgaagccgc cggaagcgga aataaagggg gcgggaaagg cggtgggcgg cacgaaaccg
    28201 aaaagcggca tcaggtatgc aaacggcgga ttcacgcacg ccgttccggc aaaaatccgc
    28261 ctgccgattc ggtgctataa tccatccgaa acagatgaca ccaaaaagga acatctcatg
    28321 aacacgcccc aatccgccat cattcccgac cacgcccaag ccggcatctt tatcgaagcc
    28381 gacttcgccg ccaaccgcct caacgatatt aaagccgcct gccgcgcttc gctcgacgcg
    28441 ttaactgcct tgaaagcccg ctttccggac gatattttgg gtctgaccat cgccttcggc
    28501 agcaaagcct gggcaacatt cggacatacg gacgaaggca gtgaaatcaa acccttctcc
    28561 gaaatgggca acgggcttgc gccgtccacg cagcacgata tgtccatcca catccaatcc
    28621 ttccgccaaa acgccgccta cgcgcttgcc caatccgttt taggcgcgtt cggcgacagc
    28681 atatgcgtcg cgtccgaaga acacggtttg cgcctgtatc aggatcgcgg actggacggt
    28741 ttcgtcgacg gcacggaaaa cccgcagggc gatgaaaatg tccgcgaagt cgccatcatc
    28801 cccgaaggaa aacccgatgc gggcggcagc tatgtcctgc tgcaaaaata cctgcacgat
    28861 ttgaaaaaat gggatgccgt ccccgtcgcc gaacaggagg cctcggtcgg acgcggcaag
    28921 gaaaccgacg acgaattcgg ccgcgatgtc cgcctgcccg attcgcacct cggccgcgtc
    28981 aacctgaaag aaaacggagt cggcctgaaa atcgtccgcc gcagcctgcc cttcggcaaa
    29041 atcagcggcg aacacggttt gatgtttacc gcatactgcc gcacgctgca caatatcgaa
    29101 gcgcaattat tgagtatgtt cggcgacacg gatggcaaaa ccgacctgct gctccggcac
    29161 ctctccgccg ccgtttcggg cggatactac tacgcgccgt ctgtcgaacg cctgcaaaac
    29221 ctctaagcca gacgatgccg tctgaaaccc tttttcagac ggcattttcc cgttttaccg
    29281 tccaaccgtt ttccgagcgc gcactatgtc tgcacccatc caagcatttg acccgctgac
    29341 cgtccccatt gccggcacca acctgattga ggcctctgcc ggcaccggca aaacctacgg
    29401 catcgccgcc ctgtttacgc gcctgatcgt attggaacaa aaaaacgtcg agcgcgtatt
    29461 ggtcgttacc ttcaccaaag ccgccaccgc cgagttgaaa acacgcctgc gcgcacgttt
    29521 ggacgatgtg ttgcaggttt tagaaagtaa agaaattgcc aaacttggag acgacacgct
    29581 ttcagacggc atcgccgcct actgcgccga acaccacaaa ggcgacacct tcctgcccgc
    29641 actcttaaaa caggctttgc aaaaagaggg ccggacgcgt ctgattgtcc gcctcaaggc
    29701 cgccatcggg caattcgaca acgccgccat ctataccatc cacggcttct gccaacgtat
    29761 cctgcgcgac tacgccttct tgtgccaagc accgttcgat gtcgaactga ccgaagaaga
    29821 cggcgaccgc ctgcttatcc cggcgcaaga tttttggcgg gaacgcgtca gcaatgatcc
    29881 ggtgcttgcc gcattggcgt ttaaacgcaa agctgtgccg caaaccgtcc ttgcccaaat
    29941 ttcgcgctat ctttcccgcc cgtacctgaa tttccgccgt ccgcaggcgg atttgaaaca
    30001 ggcgcagcgc aacgccgaaa cctcatggca aaccatctgc cgcctgctgc ccgagctgga
    30061 agccggcttt tggcgcatcc atcccgacct caacggcaac agttaccgca aaaacagctt
    30121 cggcaacctt tttaaagaac tggcacaaaa atccgccgcc ggacaactgc cctttctgga
    30181 caaagacacg cacgacagac tcttgaaact tgcgtccgac aaactcgaag ccgcccttaa
    30241 aaaaggcaaa acgcccgatg cggcagcatt tgccgaattg cagaaactgg cagacttcgg
    30301 gcgcgatttg aacgcactcg aagaagcgga agaagcaaca atgatccggc tgcaactgga
    30361 tttaatcgga tacctcaacc gcagccttgc cgagatgaaa aaatcgcgcc gcgaacgcgg
    30421 tttcgacgac ctgctgctcg atgtccacac cgcgctgacc gacaatccgc acgccgaaac
    30481 cctcgcccgc gccgttgccg aaaactggga aaccgcgctg atcgacgagt tccaagacac
    30541 cgacccgctg caatatgaaa tcttccaaaa gattttcatt gcccaaaacc gcccactgtt
    30601 tctcgtcggc gatcccaaac aggcgattta cagctttcgc ggagcggaca tttacgccta
    30661 cctgcaagcc gccggagacg cgcggcaccg ctacacgctc gccaccaact accgcagcca
    30721 cgccgcgctt atcggcagca taggcgcgct gttccgcctc aaagaacgcc cgttcgtttt
    30781 ggaaaacatc ggctattcgg aagtcggtgc ggcgcgtgcc gaaagcaggt tgtcccccga
    30841 acgtcctgcc gtacaggttc gttggctgca cgaaaacgac aatgaaaaag ccaacaaaga
    30901 cgttttgcgc cgccgtgccg ccgactattg cgccgacgaa atcgcccacg cgctcaacga
    30961 agccgccgga ggccgtctga atttcaaagg ctgcccgttg cagtcgggcg atattgccgt
    31021 gctggtacgc acgcacaacg aggcggtgat ggtttccgcc gccctgaaaa aacggcaggt
    31081 gcaaagcgtc ctgctttcgc gcgaatctgt gttcgcctcg cccgaagccg ccgccctgtc
    31141 cgcactcatc ggcttctggc tcgaaccgcg ccgcgccgga acgctgcgct ttgtcctgac
    31201 aagcggcata ttcggttatg acgcgcagca attgcacgac ttcaaccaaa acgaaagcga
    31261 gattttgcat tgggcggaat ctgcccgaac cgcactcgac aactggaatc aatacggcat
    31321 tttcgccgct atgcagcaat tttcccaaac acacggcatc gaaacgcgcc tcttaagccg
    31381 gaacaacggg cgcagcctga ccaactattt ccaactgctc gaactgcttg ccgccgaaga
    31441 cgcgcaaaac cgcaacccag ccgcgctgca caaatggttg cgcgaccaaa tcagcctggc
    31501 cggcaacaac agcggcgaca accgcgccat ccgtctggaa agcgacgaag atttggtcaa
    31561 aatcgttacc atgcacgcct cgaaaggttt gcagtatccg ctggtgtact gcccgtttgc
    31621 gtgggacgcg caagataccg ggccgtccga ctggcaaatc ctccaccaaa gcgcaaaccg
    31681 aaccgaactg ttggcaaagg cgcaactgtc ggaagacgaa cagaaacaat acgccgatga
    31741 agaaatggcg gaacgcctgc gcctgcttta tgtcgcactg acgcgtgccg aggaacagct
    31801 caacatctac gccgcgtact ccaccaatac tgccgacaac cccctcgcct acctgattga
    31861 aggctcgccc caagacagcc gtgaaaccgt ccgccgtgcc tatgcgtgtg aaaaagacgg
    31921 catcgcgatg ctcaaacgca actggcggcg cgtggcggac aacgcccctt ccggcacaaa
    31981 tttcgccttc accgaaaatg cgccgccccc tgccgcctat cgcagcaacg ccggtcaaac
    32041 cgccgaattt gccgcaaaca gcattcccga acgcggattc cgatttgtcc gccacactag
    32101 ctttaccgcc ttaagccgcc atacccaaac gcccgacggc ggcgaagaag atgcctgccc
    32161 gtccttggat gccgccgaaa cctcggtgcc ggcgatgccg tctgaaacgc cgacggcttc
    32221 agacggcata tcgatacacg attttccgaa aggcacgcag gcggggctgt gcctgcacga
    32281 aattcttgaa gatttcaaat tcggacaagc agccgccgga caggaaaccc tcattgccga
    32341 caagctgaaa aaatacggtt ttgaagaaat atggctgccc gccgttgccg aaatggcgga
    32401 agcctgccgc aaaacgccgc tgacgggggc atacgacctg tccgacatct cacccgagtg
    32461 ccgctgtccc gaaatgggct ttaccctcca caccgaagac ttcagcctca aacgcctgcg
    32521 cgactggttt gcccgcgacg acatcaggct gcccgaagtc tgccgtgccg ctgccgaaac
    32581 gctcgacttc cacaccgtca acggcttttt aaacggcttt gtcgatatgg tctgccaaga
    32641 ccccgacggc aatatctgcg tcatcgacta caaatcaaac cacctcggta cggacgcatc
    32701 cgcctacacg caacaggcaa tggacgaagc cgtcgcacac caccactatt accttcaggc
    32761 actgatttac gccgttgccg ccgcacgcta cttcaagctg cgcggacaac cgcccgccgc
    32821 cgtttccgtc cgctacctgt ttttgcgcgg attggatggc aaaggcggcg gcgtttggcg
    32881 ttgggacata gatgccgccg ctttggaaca gataaaataa cttcccgtcc tttcggacaa
    32941 caaccgcagg aaaaccctat gaatatcaaa cacctgatta ccgccgcact cattgcctca
    33001 gccgcctttg ccgcgcaggc agccccgcaa aaacccgtat ccgccgccca aaccgcgcaa
    33061 catccggccg tttggatcga tgtccgttcc gaacaggaat ttagcgaagg gcatttgcac
    33121 aacgcggtca acatccccgt cgaccaaatc gtccgccgca tacacgaagc cgcgcccgac
    33181 aaagacacgc cggtcaacct ctactgccgc agcggacggc gtgccgaagc cgcccttcaa
    33241 gagctgaaaa aagcaggtta tacaaatgtt gccaatcacg gcggttatga agacctgctc
    33301 aaaaaaggga tgaaatgacg caatgccgtc tgaaaggaca ggctttcaga cggcatacgg
    33361 cgttccggca cgcggcgcaa catcgggcgc ggcgcgcgcc gccccgtccg aagcctgata
    33421 aacaaaccga taaggaacaa tgatgtttca cgacacataa agcaccgccc tatgtgttgc
    33481 cctgatttgg aaagggttac acccctcccg aataaagtct gattctaccg tttcaaccaa
    33541 aaaggaaaaa ggaaatacga tgaaacacat actccccctg attgccgcat ccgcactctg
    33601 catttcaacc gcttcggcac accctgccgg caaaccgccc acccaaaacg aaaccgctat
    33661 gaccacgcat accctcacct cgaaatacag ctttgacgaa accgtcagcc gccttgaaac
    33721 cgccataaaa agcaaaggga tgaacatttt tgccgtcatc gaccatcagg aagccgcccg
    33781 ccgaaacggc ttaacgatgc agccggccaa agtcatcgtc ttcggcacgc ccaaagccgg
    33841 cacgccgctg atggtcaaag accccgcctt cgccctgcaa ctgcccctgc gcgtcctcgt
    33901 taccgaaaca gacggcaaag tacgcgccgc ctataccgat acgcgcgccc tcatcgccgg
    33961 cagccgcatc ggttttgacg aagtggcaaa cactttggca aacgccgaaa aactgataca
    34021 aaaaaccgta ggcgaataag cccgtcgccg acatatggcg gatggaaatc aaatgccgtc
    34081 tgaaacccga tttcagacgg catttccttt caatccctat cttcccgaca aatatcaatg
    34141 gcttcctgca aactcgaaac gccgtagatt ttcaggttcg gaaactcttt ggcgttgcgc
    34201 ggcatattgg ctttggggac gatggcgcgt ttgaagccga gtttttccgc ttctttgagc
    34261 cgctcttgtc cgcgcgcgac ggggcggact tcaccgctca aaccgatttc tccaaatgca
    34321 accatttttt cgggcagcgg gcggttgcgg aagctggaaa gcatcgcgag gatgactgcc
    34381 aaatccgccg ccggttcgcc gattttcacg ccaccgacgg cgttgaggaa cacatcctga
    34441 tcgaaacagg cgatgccgcc gtgtcggttt aacacggcga gcagcatcgc gaggcggttt
    34501 tgttccagtc cgacggtaag gcgtttgggg gtgaatccgt gcgcgtcatc gaccaatgcc
    34561 tgaatttcaa ccaaaagcgg tcggctgcct tcctgcgtaa ccaaaacgca cgaaccgggc
    34621 gtgtcgtcgc ggtagctggc aaggaagatg gcggacgggt tggacacacc tttcaaaccg
    34681 ttttccgtca tcgcgaacac gcccagttcg tttgccgcgc cgaagcggtt tttgatggcg
    34741 cgtatcatgc ggtagttgga atgttggtcg ccctcgaaat acagcacggt atcaaccatg
    34801 tgttccagca cgcgcgggcc ggcaatcgcg ccgtctttgg tcacgtgtcc gaccagtatc
    34861 atggcgatgc ccatctgttt cgccatgcgc gtcagttggg cggcacactc gcgcacctgc
    34921 gacacagaac cgggggcgga agtgatttgg tcggaataca tggtttggat ggaatcgata
    34981 accacgactt cgggctgatg ctgtttcaac gctgcttgaa tcgcttccat gcggatttcg
    35041 gcaagcaggt tcacgccttc ggtgggcagt tccaaacgct gcgcgcgcag ggcgacctgt
    35101 tgggcggatt cttcgccgga aacgtatagc actttacggc tttgcgccat tttggcgatg
    35161 gtttgcagca gcagcgtaga tttgccgatg ccggggtcgc cgccgagcag gatgaccgca
    35221 ccatcgacca aaccgccgcc caatacgcgg tcgagttcgc ccatgccggt cggattgcgc
    35281 ggcacttcgg tggcggtcac ggaggagagg gattggacgg tcgaggtatc cgccgcccaa
    35341 gattggaagc gggcgttttt cggctcgggc gcggccaagc tttcctgaag tgtgttccac
    35401 tcgccgcaat gcgggcattt gccctgccat ttcggggaag tgccgccgca ttcggtgcat
    35461 tggtaaaggg ttttaagcgt ttttgccatc ggttttccct acatctgaaa acaaaaatgc
    35521 cgtctgaaaa tagggacggt tgggattgcg ccaatacggc aacaccgccg ccgtcatgta
    35581 ctatccgtac acggcggata ttgccgcatt gccttatttt taccctaacc ggccatctct
    35641 cggcttcaaa cggcatatcg ggcagtttcc tgccggctta cccgccgtga tactgccgcg
    35701 acagctcgtg tacggtgtcg actaagattt tggcgtgttc ggggtcggcg tgttggttga
    35761 tgccgtgtcc gaggttgaag acatggccgc tgccttgtcc gtagtcggct aggatgcgtg
    35821 cgacctcggt gcggatggat tccggcgtac cgaagagggc gaacgggtcg aagttgcctt
    35881 gcagggcgac ttgcttgccg acgcggcggc gtgcttcgcc tatgttgcac gtccagtcca
    35941 agcccaatgc gtctgcgcca atttctgcca tgctttccag ccacagcccg ccgcctttgg
    36001 caaatacgat aacaggcacg cgtctgcctt cgctttcgcg tttgagtccg gcgacgatct
    36061 ggcggatgta tttgaggctg aattctttaa acgcctcatc gctcaagacg ccgccccaag
    36121 tgtcgaaaat ctgcacagcc tgcgcgcccg cgtcgatttg ggcgttgaga taggcggtaa
    36181 cggcttgagc attggtatcg aggattttgt gcagcaaatc ggggcgcgag tacatcatgg
    36241 ttttgatggt gcggaattct ttgctgctgc caccttcaac catgtagcag gcaagtgtga
    36301 acgggctgcc ggagaagccg ataagcggta cgcggccgtc cagtgcttta cggatggagc
    36361 tgacggcatc aaagacgtat tgcagttttt ccatatcggg gacgtgcaat ttggcaatgt
    36421 cgtcctcgtg ttgcagggcg cgtttgaatt tcgggccttc gccttcggca aaatacagtc
    36481 ccaagcccat tgcgtcaggg acggtcagga tgtcggaaaa caaaatcgcc gcgtccaaat
    36541 cgaaacgttc caaaggttgg atggtaactt cggtcgccaa ttcggtgttt ttgcacaaat
    36601 cgaggaagct gcccgctttc gcgcgtgtgg ctttgtattc gggcagataa cgccccgcct
    36661 gacgcatcat ccaaatcggc gtgtattcga cgggctgttt aagcagggcg cggaggaagg
    36721 tatcgttttt taaaagagtc atacgggttt tctgtcagtc tgtttgtcaa gtgaaatggt
    36781 tttcagacgg catttcaacg atgccgtctg aaggtttcaa caggtttcgg cggaaggtcg
    36841 gttttcctcg gcaacgcttg ccaaaaccaa gttgcgctgc gcctccgcct tctgcggttc
    36901 tccggtttcg tcaaaaacct ttgccaaaac caaacgcgcg gaaatactcg gctttaatgc
    36961 aatgctcgct tcaaggtagc cttttgcctt gccccaaagt ttgcggccgt aggcgagccg
    37021 accgagatac atcagcagaa gcgcgttatc gggctgttct ttcagccaag catcggcaaa
    37081 atcgatggct ttctgctgat cgcgttcgcc caaaaagcgc acgctttcga caaaggcttc
    37141 caaaagctcg gggcggcggt tgtgcggata atgctgtttg acccatttga ccgcatcggc
    37201 atacagtccc aaacgttcgt acttttccgc aaccgatacg ctcaattccc cgtttttgag
    37261 gctgtcggga atccgcttca ggcaggtttt caaagcggcg gcatcggcag catccgccag
    37321 ctggcggcgg tatgcccaat tttgataccg ttccatttcc gatttgccca acgcgcccgc
    37381 cttggaaagt ttttcggttt ttgccagaac ctgcaacgcg tcgcccctgt cgaaagcgta
    37441 acgaagttgc agacgcacga ggcgcgtaag gttggcattc atcttcgccg ccgcatgaag
    37501 attggcttcc gccgcttcgt aatcgcgccg gttcaacgcc gattccgcca acaaaagata
    37561 acgggaaagc tgctgctttt ccggcagttt ggcgatttcc gcaagataac ggtcgcgcag
    37621 ctcgatgttt tccatctgcc cggcggcatg tgcgcccaac atcaatgcca aagtccggtt
    37681 gtctccggcc tctttgttga ccaacacgcg tgaggcttct agttccgcct tttcaaaacg
    37741 cccttcaaaa tacgccaaac ccgccttgtt caaggcaagc gcggccttgc ggcctttacg
    37801 cgccgaaccg aaacgctgca tcttttcggg gatattgaat acgccgataa tgaatttgaa
    37861 caggaaatac cacaccacga cggcaatcag cgaacctaac acaaaggcgt gcaggttgat
    37921 tctgagcatg gtctgtccga gtacgatata cacgtcgccg gtgtaaatgc ccgaagccag
    37981 tgccagtccg acggcggcgg caaacaggac gacaatccag actaccgttt tcatgcgcgt
    38041 tctcctttag cggacggcga acgccctgcc ttttccggta cgggctgttt ttttgcaggc
    38101 tgttccggtt tgcgttccga aggcagggac ggtgcttcta tggtcttcac atccgatgcg
    38161 gcagccggtt cggaagctgt gttttcgttt gccggttcgg aagtcgcctg ttccgcttct
    38221 tgattttccg ccgtcgtcat gcgcgtaccg tcgcgatagg cgcggacggc atttaggctg
    38281 tttttcaaac cgtcatccgc cgtcatgcgc acatccaacg ccttcaattc cgccaattcc
    38341 ttcaaccacg actgcgtggc gggggatttg gcatcaaaat actgtctgac ggcggtttcg
    38401 gcattgttca aatcgccctg atagacttcg ctgttgcgct gcattaatgc agtccgcgca
    38461 tccaaaaggc ggagacgcag gttttcacgc acaaaatagg cctgttcggg agaaatcagc
    38521 atggcatcgt tgttttccaa acggcggatt tcgaccagcc ccttcaatgt gccgagggat
    38581 ttttcccata cgttctgcca ccatgaagcg gaaacggctt cgttgcgtgc ctgtacgctc
    38641 ggtttcaata cgccgtccaa aatcagcggc agtccggata cggcgatttc cagcctgtcg
    38701 aggcgcaatg ccgtgccgga aatatcgaca tagggacggt ttttcagttc cgccaagtcg
    38761 ctgctgaccg cctgcttgat cggcagaagc tctgcctgat tgaaacggga caggcggctg
    38821 tcgatatgct ccaatacgcc gactgccgtt tggatattgc cagtcaacac cagctgttgc
    38881 gccgccagat tgagtatggt ctcggtttcg tccaccagcc aatcggcgcg tcctttggtc
    38941 aactcgcgat aggatttttg catttccaag atttgttcgc cgtttgcttt gacgtttccg
    39001 tccaaacggt cgagctctga ttgtatggcg gcttgccggt tgaggttgtc tttgagcagg
    39061 gcggcgtttt ccgactcgcc caaagcggct ttgtcgattt tttggttgaa tgccagctct
    39121 tggtttttca agacattctg tccttggaca aacaaaaaac cacttgtacc caaacccaat
    39181 gccgccaata ccagggcgca gactgccaaa gcgttgctgc cggactgttt gacgataaaa
    39241 ggagcggcag gaatgtgtac ttctgtttcg ttttctttgc gtggggaaga ggtttcagac
    39301 ggcatttctt ttgatgcctg tatctcgcgt acgggttcgg atgatttgtt ttcaggttcg
    39361 cccaccgttt tgctccttat taatttgagg ttccgggaaa gaccatgccg tctgaaacgg
    39421 aaatggaaga atgggaaagc gcggcttcca gcgtagggac ggtttcgacc gaacacacgc
    39481 cttcgcgctt caatgcctcc gcaatgcgcg gatgatgggt aaagtatagc aaggatttga
    39541 agaatcggga aaattgcggc ggaagctgcc cgaacagcaa gcgcaccagc tcggtcgacg
    39601 taatataggc ggcggcaata ttttcggttt ggaaattttg aaagttcaaa ggtttatgcc
    39661 gtctgaaata gacttctgcc atctccgtcc gaaaaccttt ctcctgcaag gcattcatca
    39721 gaaaatcccg cccgccgtgt ccgcgcacaa acaatacgcg tgcaccttcg ggcagactgt
    39781 tccaaaccgg caggcgcaaa accgcctcgc tgtcgtttcc gtcatgaggc gcaatgaccg
    39841 ttctgcccaa atagcgttcc aatgcgcggc ggctgccctg ccctacggca atgtgtatct
    39901 ttatgccgtc tgaaaggtta aggtacggga cggcggtttc aacggcggtc ggactgaccc
    39961 aaaacacggc atccgcacgg gcatattgtt cggacaaaag ttccagtccg gcagcatctg
    40021 cttcgatttc aatcgggctc aacacttccg cctgccatcc ggcacgacgg caggtttcca
    40081 catcgtcctt cgccctgccc gacggtcgga caatcagcat gatgggtttc gctgtttcca
    40141 tcgccttatc cgccccgatt accgggaaaa atcagcatcg ttgaaattcg cctgcgcggt
    40201 ttgcggcgta cagccgaccc gtgcgacttc cttgccccta cgctccacaa cgacaccgct
    40261 gctttccgta tgagtcacgg aatcgaagcc tgtccatatg gtgtacataa attcgccgtt
    40321 ttggaatttc atcgttgccc aacgaccgct gcccatacct tgccacctgt cggaacgccc
    40381 cgacagatca gctttgctgt tgcgtatggc aatttctttt tttgccgcac tgccgaacga
    40441 atattcgtaa agattgcggt tgattttttg aacttctata tatttgttgt tgtccgtttt
    40501 acaggaaaac accgtatccg cccacaccgt ttgggcggca gccgacagaa gcaatacggc
    40561 agaaaggaac atttttttca tcgtatttcc ttaatggttg aaaccccgcc actcggacat
    40621 ccgtccttcg tggcagcagc atcagatttt atttgagagg ggtgtaaccc cttccaaatt
    40681 agggcaacac atagggcaac gctttatgtg tcgtcctgtg tgttgaaaca tcattgcccg
    40741 tatcctgaaa tagaaaagaa aatgcccgtt ttacggtctt ctttccgaca aaatgccgtc
    40801 tgaaacggat ttcagacggc atggtcgaaa attattgcgc ggcaagcggc ttgagcagca
    40861 aggtttcgcc gctttgcaca actttttcaa gcaggtcgat attgggatgc ttgccgggat
    40921 gggcgcgcgc gtcggcaacg tgttcggcaa accatatcaa accctgttct gccgcttctt
    40981 tgtccaattt tccgtcaaaa cgttccgcca aagcattgta cagcttgagc gaaccgagct
    41041 taccctgaac ggcgggaatg tggtgaaccg ttttgccgtc ggcatcctga acgtccaaac
    41101 cgcccagatg gtcgattgaa ggaaatgtgg cgagatagtc ttggaaattc atatcaaatc
    41161 cgaaaatcag tcagttagcg cgtgccgaaa atcttgtcgc ccgcatcgcc caagccgggg
    41221 atgatgtagc cgttctcgtt caagtggctg tcgagcgcgg cggtgtaaat cgtaacgtcg
    41281 ggatgcgcgt cgttgaccgc cttcacaccc tcgggcgcgg caaccagcac cagtgccttg
    41341 atgtttttgc agcccttggc tttcaacagg tcgatagttg ccaccatcga accgcccgtc
    41401 gccagcatcg ggtcgataat cagggcagga cgttcgtcca tgctgtccac gaatttctcg
    41461 aagtaggaaa cgggtttcag cgtttcctcg tcgcgctgca gtccgactac gctgattttg
    41521 gcagtcggaa tcaggtcgag tacgccgtca agcataccca aacctgcacg caggatggga
    41581 acgacggtca atgttttgcc cttgatgcga tcgccttcaa tctgaccgca ccatccgtcg
    41641 ataaggtatt tttcgatttc aaaatcacgg cttgcctcgt atgccatcag gcgcgccagc
    41701 tcgatggcaa gcgtccggaa tttgtaggtg ctgcaatctg cctccctcat cagggttaat
    41761 ttgtggcgga cgagcggatg gttgataacg ttaacgttca tcgcggtgtt ccagtgtgat
    41821 tttgagacgc gccattatac ttttttttga tttgccggaa aagaaaatgc cgtctgaaac
    41881 acgttcagac ggcattgccg aatccatcag gctttcagca gctcttgcag ctcgcctgcc
    41941 tcatacattt ccatcaggat gtccgaaccg ccgacaaact cgccgttcac ataaagttgg
    42001 gggatggtcg gccagtcgct gtattcttta ataccttggc gtacttcggg attttccaat
    42061 acgttgacgg taacgtaatc ggtgcagcct gccgcgttca ggatttgcac ggcgcgggaa
    42121 gagaaaccgc attgcggaaa ctgcttcgta cctttcataa acaatacgac gcggtgtgtc
    42181 gttactactt ctttaatttg gtcgtggatg gaagccatcg cttattcctt ttgataaagt
    42241 tgacaaaatt tgtcaggtag gacgcattat acgcaaaagc ggaaacttcc cacaatattt
    42301 gccgtatatc gcgcagtatc aatgttgtga acggaaatat tcccactcct cgacaatcct
    42361 gctgtccggc aaatggcaca gggcatatcc gccgccgctt tcgtcttttt ttacttcccg
    42421 tctgccgcct ttggcaatgc agaatttgga agcggaattt gccatgcctg tccgaaacgt
    42481 gttggcaggc agccggcagc agcgcggaca tgcatacgga tgaattgcct tcatggtttt
    42541 ccttatcgtc ggtacagggt cagacgaccc gcatatcggg tactttttta aacagccggt
    42601 aaaagttttc cgtcgtatat gccgcaacct gttccaatgt ttggttccgc aatttggcga
    42661 tatgttcggc ggtatagcgc acaaaagccg gttcgttctg cctgccgcgt ttgggaacgg
    42721 gggcgaggaa cggcgcatcg gtttccacca aaatgcggtc gtccggcaca tatttcgccg
    42781 cctcctgaac caagggggca tttttaaagg taacgattcc cgagaaagaa atataaagcc
    42841 ccaaatccat ggcagcccgg gcaaaggctg tgtcttcgga aaaacagtgg ataacgcccg
    42901 aattaacccg gcattctttc aggatagaca aggtgtccgc cgccgcatca cgcgtatgga
    42961 cgataacggg cagtccggtt tgattggctg cttcgatgtg gtctgcaaag cgtttgtgtt
    43021 gccaggacaa atcgcctttg caccagtaat aatccaagcc cgtctcgcca atgccgacca
    43081 ctttcggatg ggcggctgca gcaaccattt ccgcaataga aaattcttcg gcttccttgc
    43141 tgtcgggatg tacgcctatg gtgcaataga tgtgttcgtg cgcttcggcg atggcaaaga
    43201 cttcggagaa gctttcccta ctgacgctga tggcgagcac ctgccccacg ccgtttgctt
    43261 ccatgttgga caaaacttcg ggcaggcgtt ctttcaaacc ttcaaaattg aggtggcagt
    43321 gcgaatcgat gatatgcatg gcgttacatg gtaaacgtag gtctgctgcc gccgattgtg
    43381 ttgcccagtt cgacttcgat ctggtctttg actttcaggc agttttcgtg ttttgtgaat
    43441 gccagcccca cccctttggg tttggaggaa gtacgcgcag gattgatcca ggcgactttg
    43501 gtcggcagga acagtttggg gaagttgagg atttctacgg caagcagaat atcgtccccg
    43561 atggaaaata cgtcgttggt ctgcacaaac agaccgccgt gttccaaaaa cggcatatag
    43621 gagctgtaca gcagattcat gtctttcagc tgcaacgaca tcatttttgc cggaatattt
    43681 tgtccgtctg acataattca cccgttctct ttcttcaatt ccaaataatt gatgagcaga
    43741 tgctcgatct gcattttgac atttaaagta tgaaacccgt aaggggcaag ctgtttgagc
    43801 atatcctccg ccgcaaatac attgcgcggg cggaaaccgg atgccgtctg aagcagcctg
    43861 tcttcataag cgggataata gacgggtttc atgtgttgca ggcacaatcc caaatcgacc
    43921 agccatttct gcatccaccc gacaaatacg tcgagcggaa gtttctcctt atcgaaaagc
    43981 gcggcgtaat ccaaaatctt caacaacctt ggttctgcca aaatatccaa cagtttaatc
    44041 cgcaacgcac ggacaccgtc cgcctcatca aacagcggcg cacccgaatg gaacgccaaa
    44101 aattcttccg gctccgccac acccctgtcg cgcagatatg ccaatgcttc cccatgcgaa
    44161 ggagcgggca aaaccatctt ccggcagcga cttttaatgg tcggtaaaac cttgtccgcc
    44221 gcgtggctga ccagcaaaaa gaccacttgt ggcggcggtt cttccaacac tttcaacaaa
    44281 ctgttggcgg cttggacatt catactttcc gcaggatgaa tcagaatcac gcgcaaaccg
    44341 ccccgtaccg aagtcaggta cacattatcg atgatttccc tgacggcatc gattttgatc
    44401 tgcaacagtt tgcgtccgtt ttcgggttcg tccgacaagg gggtgatttc gtaaaaatcg
    44461 ggatggcttc cctgtccaaa cagatggcag gacatacatt cgccacaggg tttgcagccc
    44521 ggtgcagggg tttcgcacaa cagtgccttc gccgcaaagc gggcaaatgt agttttcccc
    44581 gtatcttttt tgccggcaaa cagccatgca ttgggacgac gctcccaatg ttccgcaatc
    44641 tgccgccatt gctcattatg ccacggataa atcatttcaa accatccgtc cggaaatcca
    44701 taccgccatc agagcagttc caaatcgggg ctggaacttt gcgcgcggcg gctgcgcaac
    44761 tgtaccgcca aacgcaaatc gtgtgcggaa tcggcatttt tcaatgcttc ttgcagggaa
    44821 atatcgcctt tttcatacaa ttggtaaagg tgttgatcga aggtctgcat acccagggta
    44881 gtggattttt tcatcacttc tttgatttca tggatgttgc cgttgtgaat caactccgaa
    44941 atcaggggcg aattgagcag cacctcgact gccgccaccc tgcccttgcc gccgtctcgc
    45001 ggaacgaggc gttgcgaaat aaacgcctga aggttgagcg acaaatccgt cagcaattgt
    45061 tcgcgccgct cctcggggaa aaagttgatg atgcggtcga gtgcctgatt ggtgctgttg
    45121 gcgtgcagcg tcgccataca caaatgcccc gtttcggcaa aggcaatggc gtagtccatc
    45181 gtttcgcggt cgcggatttc gccgataagg atgacatccg gcgcctgacg cagcgtgttt
    45241 ttcaacgccg ccatccagtt ttccgtatcc acgccgacct cgcgctgggt gatgatgcag
    45301 tttttgtgtt cgtggacaaa ctcgatcggg tcttcgatgg tgatgatatg tccgaacgaa
    45361 ttttcattgc ggtaatcgat aagcgaggca agcgaagtcg atttgcccga gccggtgccg
    45421 ccgacaaaaa taaccagccc gcgttttttc agcgcgacat ccttcaagac cggcggcagg
    45481 ttcaggcttt caaacttggg aatcttgctg gtaatcgcac ggaataccaa cgccgtcgcg
    45541 ccgcgctgta tcatcgcatt gacgcggaag cggctggtgt ccggcaggct gatggcgaag
    45601 ttgcactcgt tggtcgatga aaattcttcc gcctgcttcg cactcataat cgaaaaggcg
    45661 atttccatac atttttccgc cgtcagcggt tcgtccgtga tgcgggtgat tttgccgtcc
    45721 agcttcattg cgggcgggaa atgggtcgtc acaaacaggt cggaaccttt gtttttgttc
    45781 atatggcgca gccatgcgaa cagttcttcc tttgcggtca tattttcatc ggtaaacatc
    45841 ctcgtcccct aatcttcaaa caatctgtac cttcccattc taccaaaaac ccgccgtgcc
    45901 gtctgaaacc gtttcagacg gcacggacaa accacctcca ctcaatcctg ccgtgttagc
    45961 gtgtataatc aggcattcta tccgaaattc acaacaggtc agcaatatgt ctttgaaaac
    46021 agtagccgtt atcggcgcaa tggaacaaga aatcgagctt ttgcgcgaga tgatggaaaa
    46081 tgtcaaagcc gtctctttcg gcaagtttgc cgcctatgaa ggcgaattag cgggaaaacg
    46141 catggtgctt gcattgagcg gcatcggcaa ggtcaacgcg gcggttgcaa cggcttggct
    46201 tatccatcaa ttcgcaccgg actgcgttat caacaccggc agcgcgggcg gcttgggcaa
    46261 gggtttgaaa gtcggcgatg tcgtggtcgg cacagaaatc gcgcaccacg atgtcgatgt
    46321 aaccgcattc ggctatgttt ggggacaagt gccgcaactg cccgccgtat ttgtttcaga
    46381 cggcctcttg gtcggcaaag caaaacaggc ggcggaagtg tttgaaggcg cggcggtaga
    46441 acaaggcctg attgtcagcg gcgaccgctt cgtccacagc agcgaaggcg tggcggaaat
    46501 ccgcaagcac ttccccaaag tcaaagcagt ggaaatggaa gcggcggcga ttgcccaaac
    46561 ctgtcatcag ttggaaacgc ctttcgtcat catccgcgcg gtttccgatt cggcggacga
    46621 aaaggcagac atcagctttg acgaattttt gaaaacggcg gcggcaagtt ctgcaaaaat
    46681 ggtggcagaa atcgtcaaat ctttataaaa cctttatcaa atcatgccaa aacagcggtt
    46741 ttccgttaga atatcgggct atttccacgt tgcacccgat attcggacgc aacgccgttt
    46801 gttcagacgg catttcccaa acccactgtc cgacattcaa aacaactcta atgaaaaata
    46861 tccgaaattt ctccatcatt gcccacatcg accacggcaa atcgacgctt gccgaccgct
    46921 tcatccaata ttgcggcggt ttggatttgc gcgaaatgag tacgcaggta ctcgattcca
    46981 tggacatcga aaaagagcgc ggcattacca tcaaagcgca aaccgccgcg ctcaactata
    47041 aagcgcgcga cgggcaggtg tatcagctta atttgattga cacgccggga cacgtcgact
    47101 tctcttacga agtttcccgt tcgctgtcgg cttgcgaagg cgcgcttttg gtcgttgacg
    47161 cgtcgcaggg cgtggaagcg caaaccgtag cgaactgtta caccgcgatt gatttgggcg
    47221 tggaggttgt gcctgttttg aacaaaatcg acctgccagc cgccgacccc gaacgcgtgg
    47281 agcaggaaat tgaagacatc atcggcatcg atgcggtcgg cgcggtgcag tgttccgcca
    47341 aaagcggcat cggcgtggaa gacgttttgg aagaaatcgt tgccaaaatt cctgcgccga
    47401 ccggcgatga aaacgcgccg ctgcaagccg tgattgtcga ttcgtggttt gacaactacg
    47461 tcggcgtggt catgctgatt cgagtgaaaa acggcaccat caaactgaaa gacaaagtgc
    47521 gctttatgag caccaaggcg gaaacgcagg ttgagcagtt gggcgtattc acaccgaaat
    47581 cggttcaaaa acaagaactc aaagccggcg aagtgggctt tttgattacc ggcgtaaaag
    47641 aattgggtca ggcgaaagtc ggcgatacgg ttacactggt tgccaaccct gccactgagc
    47701 cgctgcccgg cttccaagaa gtacaaagcc aagtattcgc cggactttat cccgtggaaa
    47761 gccacgacta cgaagccttg cgcgacgctt tggaaaaatt gcagcttaac gacgcttcgt
    47821 tgaaattcga gcctgaagtt tcccaggcat tgggcttcgg cttccgttgc ggcttcttgg
    47881 gtctgttgca cttggaaatc gtgcaggaac gcttggagcg cgagttcgat atggatttga
    47941 ttaccaccgc gccgacggtg gtttacgaag tcgtgttgaa aagcggcgaa aaaatcgaag
    48001 ttgaaaaccc gtccaaactg cccgacatcg gcagcatcga aaccatactc gagccgatta
    48061 ttaccgcgac catccttgtg ccgcaggaat acgtcggcaa cgtcatgact ttgtgtaacc
    48121 aaaagcgcgg cgttcaggtc aatatgcagt acatgggccg ccaagtcatg ctgacttacg
    48181 atttgcccat gaacgaagtc gtcatggact tcttcgacaa actcaaatcc acttcgcgcg
    48241 gttatgcttc gttggactac catttcaaag agttccaacc gtctgatttg attaagctgg
    48301 atattatggt caacggcgaa aaagtcgatg cattaagcct gattgtgcac cgtcaaagcg
    48361 cggttcaccg aggccgcgag ctggcatcaa aaatgcgcga gctgattccg cgccaaatgt
    48421 tcgacatcgc cgtccaagcc gccatcggca gccagattat cgcccgcgaa aacgtcaaag
    48481 ccctgcgtaa aaacgttttg gcgaaatgtt acggcggcga tattacgcgt aagaaaaaac
    48541 tgcttgaaaa acaaaaggca ggtaaacgcc gtatgaaaca agtgggcaat gtggaaattc
    48601 cgcaaagcgc gttcttggcg attttgcagg tgagcgacaa ataactctgg cacacagggc
    48661 ggcgcgtcgg gaacacccct caaaccgccc tgatttcagg ggcacgcgcc cgcaaacaaa
    48721 ttttgcaatt tgccgcctta aaacggttgc acggacaggc gggtttttaa gcattaagga
    48781 acaacaatga acacaatgct aatgtcgggc gcggctgccg cgctgcttgc cggcatcatc
    48841 ctttatttca aaagcgacaa aaagcggcag gaaaacggtg aatggagttc cggtctggaa
    48901 tacgcctata tcctgacggc ggtcggcgtg tttgccgctt tgtccctgtt tatgagcttt
    48961 accgccgttt tcctgatttt cgttgtattg tgcggtacgg cttggggggt atacaaatac
    49021 cgcctgaaga ctcatcccga aatctcggaa agcagccact tcggcgatta tttcggcagt
    49081 ttcttcccta ccgttttggt attgttcctc atccggtcgt ttattgccga accgttccaa
    49141 atcccgtcca gctcgatgcg cccgggcctg attaagggcg atttcatttt ggtcggcaaa
    49201 ttttcctacg gcctgcgcgt acccgtttta aacaatatat ttattcctac aggcaaaatc
    49261 gaacggggcg atgtggtcgt ttttaattat cctttgcaac cggagatgac ctacatcaag
    49321 cgtattgtcg gcattccggg cgatgtggtc gaatatcgga ataaagtttt gacggtaaac
    49381 ggaaaacctg cttccgatat tcctgacggc acataccgtt atcccgacga caccgaccca
    49441 tccgaaatcc acaatacgga tatgttccac agcggtttgg acggcaaatc cttcaatatt
    49501 ctgaaaaaag aaggacagcc tgccgtttcc ctgcccgtat tgggcaaata tacctccgat
    49561 attatgtctg aaaacggata ttccatagag caaagcggtt tggaacactg ccaatatgcc
    49621 gacgacggca gcggtttcgt gtgcaaagtt cccgaaggac gctatttcgc tatgggcgac
    49681 aaccgcgaca acagtgccga ttcgcgctac tggggatttg tggatgacaa gctggttgtc
    49741 ggcaaggcaa tgttcatttt gatgaacttc ggcgatttcg gcagggccgg tacggcaatc
    49801 cgttaggccg gaaaattacg ccggaacatc aaaaatgcca tccgaaccct gtttcagacg
    49861 gcatttttat catcaaacgc cttgtttccc tgccggcagc ctcggggata atatagtgga
    49921 ttaaatttaa tccactatat ttccgtttgt gtggaaaccc atcccattga ataaagggca
    49981 tttcgtccga ctgacggtgt gggcggcata tggaaatacg gacattattt tagttgcatt
    50041 gccattccag cggaggcggc ttttttgatt ctgcccgccc cgatgccgtc tgaaagggct
    50101 tcagacggca ttttccgctc cgcgccgttg ctggcgtgtt tggtcttgcc ggttttgact
    50161 gcctgccagt tcaatcgtac gcaggtttgc cacggcagaa aatctgatgc cggacggagt
    50221 caaggctcgg actgctgttt cagcgaaccg aggtaaattg ccgaaagcgt cagcgccacc
    50281 cctgcccact gcacgccttc aatcggtttg cccaacccga aataatcgat gaacagggcg
    50341 gcaaccggtt cggacaaaag cagcagcccc gtcagcgaca gcgaaagcag cggaatcgca
    50401 taggcaacca tcgcccacgc gaagcactgc atcaccacgc cgtacacaag caccaaaccc
    50461 gcatctttcc aagtcgtcgg ataaagcgcg ccgccatcca tcagcaatgc cggaacaacc
    50521 agcgataccg cgccgcccaa actcaaaatc atcattgaag ggaaaagcgc caccgtctcg
    50581 atttcatggg ttttgcggac gaacaccatc gacagggcga gcatcagtcc cgataccaaa
    50641 ccgctgacga atccccaaac cgcattaccg ttgtagccga attccgcacc ggcaatcatc
    50701 gccacgccgg caactgccga tattaagcct gcctttttca gcccgctcaa acgctcgccg
    50761 aaaaagaaaa caccgattgc cgacaagaaa aagatttgca ggctgttgag cagggtggaa
    50821 atacccggcc cgaccgcgtg tatgctttcg tgccacaacg ccaaatcgaa agcgagaaac
    50881 acgcccgccg tcagggcata tcggacggtt ttcctgtttt ttgggaattt ttgcctgaaa
    50941 aaccgtgcta aaaaccaaaa tacgaacacc gaaatcagca accgccaaaa tgcgattgca
    51001 tacgaaccga cggggacgga tctgacaatc agactgccca gaccgaacac cacgcaaccc
    51061 agcaccagca atggtgcggc atattttttg ccgttaccga ccacaatatc ccctcccttc
    51121 cccatacggc tgccgcgccg acggcaagaa tgccgccatt ccgaaaatat gccgtctgaa
    51181 gcccaaagcc ccggctgcac ccggacaaac ggctgatttt acgggcaggc cgactatttt
    51241 ccgttggggc aaaaaaccgt cctgccggat aaaaccc
//
LOCUS       DAXHMX010000002        36748 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHMX010000002 DAXHMX010000000
VERSION     DAXHMX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.002"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..1183)
                     /locus_tag="ACFM4S_000057"
                     /pseudo
     CDS             complement(1..1183)
                     /locus_tag="ACFM4S_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1329..2033
                     /locus_tag="ACFM4S_000058"
     CDS             1329..2033
                     /locus_tag="ACFM4S_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6049831.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKLKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(2097..2663)
                     /gene="dcd"
                     /locus_tag="ACFM4S_000059"
     CDS             complement(2097..2663)
                     /gene="dcd"
                     /locus_tag="ACFM4S_000059"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6049832.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICKTSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2729..3145)
                     /locus_tag="ACFM4S_000060"
     CDS             complement(2729..3145)
                     /locus_tag="ACFM4S_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6049833.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGGVGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVSANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIQEVKLPKNK"
     gene            complement(3207..4106)
                     /locus_tag="ACFM4S_000061"
     CDS             complement(3207..4106)
                     /locus_tag="ACFM4S_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6049834.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     AAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(4137..5594)
                     /gene="der"
                     /locus_tag="ACFM4S_000062"
     CDS             complement(4137..5594)
                     /gene="der"
                     /locus_tag="ACFM4S_000062"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6049835.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREDRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5744..6373)
                     /locus_tag="ACFM4S_000063"
     CDS             complement(5744..6373)
                     /locus_tag="ACFM4S_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6049836.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6374..7669)
                     /gene="hisS"
                     /locus_tag="ACFM4S_000064"
     CDS             complement(6374..7669)
                     /gene="hisS"
                     /locus_tag="ACFM4S_000064"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6049837.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQVFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7798..8454)
                     /locus_tag="ACFM4S_000065"
     CDS             complement(7798..8454)
                     /locus_tag="ACFM4S_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6049838.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVGQIRQARAE
                     "
     gene            complement(8512..8703)
                     /locus_tag="ACFM4S_000066"
     CDS             complement(8512..8703)
                     /locus_tag="ACFM4S_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049839.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIGSAWHFLDAACWISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(8736..9272)
                     /locus_tag="ACFM4S_000067"
     CDS             complement(8736..9272)
                     /locus_tag="ACFM4S_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049840.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9440..9598)
                     /locus_tag="ACFM4S_000068"
     CDS             complement(9440..9598)
                     /locus_tag="ACFM4S_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049841.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLERTGAEVYINGRRVYHNEA
                     EMASASLR"
     gene            complement(9755..10174)
                     /locus_tag="ACFM4S_000069"
     CDS             complement(9755..10174)
                     /locus_tag="ACFM4S_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049842.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(10232..10597)
                     /locus_tag="ACFM4S_000070"
     CDS             complement(10232..10597)
                     /locus_tag="ACFM4S_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049843.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(10579..11124)
                     /locus_tag="ACFM4S_000071"
     CDS             complement(10579..11124)
                     /locus_tag="ACFM4S_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049844.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(11287..11560)
                     /locus_tag="ACFM4S_000072"
                     /pseudo
     CDS             complement(11287..11560)
                     /locus_tag="ACFM4S_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12122..12595)
                     /locus_tag="ACFM4S_000073"
     CDS             complement(12122..12595)
                     /locus_tag="ACFM4S_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049845.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            complement(12638..13507)
                     /locus_tag="ACFM4S_000074"
     CDS             complement(12638..13507)
                     /locus_tag="ACFM4S_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6049846.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAALVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNSKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKLGVQHTF"
     gene            13672..14595
                     /locus_tag="ACFM4S_000075"
     CDS             13672..14595
                     /locus_tag="ACFM4S_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6049847.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(14673..15314)
                     /locus_tag="ACFM4S_000076"
     CDS             complement(14673..15314)
                     /locus_tag="ACFM4S_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6049848.1"
                     /translation="MQKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGHGETQDCIAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15369..16160)
                     /locus_tag="ACFM4S_000077"
     CDS             complement(15369..16160)
                     /locus_tag="ACFM4S_000077"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6049849.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARSLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLADLKASNPNNGKHFHL"
     gene            16326..16401
                     /locus_tag="ACFM4S_000078"
     tRNA            16326..16401
                     /locus_tag="ACFM4S_000078"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16359..16361,aa:Thr,seq:tgt)
     gene            16436..16511
                     /locus_tag="ACFM4S_000079"
     tRNA            16436..16511
                     /locus_tag="ACFM4S_000079"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16469..16471,aa:Thr,seq:tgt)
     gene            16653..17444
                     /gene="panB"
                     /locus_tag="ACFM4S_000080"
     CDS             16653..17444
                     /gene="panB"
                     /locus_tag="ACFM4S_000080"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6049850.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVLAELAKKVTETVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            17567..18403
                     /gene="panC"
                     /locus_tag="ACFM4S_000081"
     CDS             17567..18403
                     /gene="panC"
                     /locus_tag="ACFM4S_000081"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6049851.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYCELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            18568..20412
                     /locus_tag="ACFM4S_000082"
     CDS             18568..20412
                     /locus_tag="ACFM4S_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6049852.1"
                     /translation="MLPNRFKMLTVLTAALIAGQVSAAGSGAGDMKQPKEVGKVLRKH
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTEEQRSRAALTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRFAPDNAQIMNNLGYSLLSDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            20553..21134
                     /locus_tag="ACFM4S_000083"
     CDS             20553..21134
                     /locus_tag="ACFM4S_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6049853.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETLERCAARTR"
     gene            21144..21989
                     /gene="ispE"
                     /locus_tag="ACFM4S_000084"
     CDS             21144..21989
                     /gene="ispE"
                     /locus_tag="ACFM4S_000084"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6049854.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            21997..22072
                     /locus_tag="ACFM4S_000085"
     tRNA            21997..22072
                     /locus_tag="ACFM4S_000085"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22030..22032,aa:Gln,seq:ttg)
     gene            22085..22160
                     /locus_tag="ACFM4S_000086"
     tRNA            22085..22160
                     /locus_tag="ACFM4S_000086"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22118..22120,aa:Gln,seq:ttg)
     gene            22169..22244
                     /locus_tag="ACFM4S_000087"
     tRNA            22169..22244
                     /locus_tag="ACFM4S_000087"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22202..22204,aa:Gln,seq:ttg)
     gene            22411..23394
                     /locus_tag="ACFM4S_000088"
     CDS             22411..23394
                     /locus_tag="ACFM4S_000088"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6049855.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            23461..24033
                     /locus_tag="ACFM4S_000089"
     CDS             23461..24033
                     /locus_tag="ACFM4S_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6049856.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(24450..24668)
                     /locus_tag="ACFM4S_000090"
     CDS             complement(24450..24668)
                     /locus_tag="ACFM4S_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049857.1"
                     /translation="MRAVEICGFFFAGDEGAGSGALQVEIGGGFARPIELITFARTGK
                     VFCAEDEFELFKVNLVFTKDFGKELFEF"
     gene            24672..26300
                     /locus_tag="ACFM4S_000091"
     CDS             24672..26300
                     /locus_tag="ACFM4S_000091"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6049858.1"
                     /translation="MFIEAENLEALKILQKSYAGSVKMIYIDPPYNTGNDSFVYPDKF
                     SESREEYARRVGDTDDAGYLKRDGVFQGAWRKNGKDSGHYHSNWLSMMLPRLHLAKTL
                     LREDGVIFISIDDNEQAQLKLLCDEVFGAENFVGQFPWRKRTAKSDVPFGVSADYEYL
                     LAYARSNAFIASIDAEERRYFETDDFPNRPWRTHDLTTQRNASERPNSFFTMVNPKTG
                     KEYPANPNATWRVTKDTFQEYYNAGKIVFPDDYDFLKIKNPVMRYFKDDDMKKDGERF
                     GRKAVSTRLPENVGMSLNGTKEITELFKSKLFDFPKPSSLIKYLLQILHVSDGLILDF
                     FSGSGTTAHAVMQLNAEDGGNRRFICVQLPEETDEKSEARKAGFDTIAEIAKERIRRA
                     GRQISDGLQDGSETDTGFKVFKLAESGFKQWRQPEQSDTEALQRELSLNIDSVLSETS
                     SENLLYELMLRMGLKLTCKVSFLDDVYFVEDEDTGGLYAFLLKRVDQGLIDAVLAKHP
                     AKVAALDRLFEGDDALKSNTVLQMKDAGVMFECV"
     gene            26535..29186
                     /locus_tag="ACFM4S_000092"
     CDS             26535..29186
                     /locus_tag="ACFM4S_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6049859.1"
                     /translation="MELKFEELPYQLDAVNAVANLFAGQPNHARTFDLTSQGTGRFVG
                     NGLDLDWETLGLNLNTVQKQNGQPETEIGAHGLNFSLEMETGTGKTYVYLRTIYELNR
                     SYGWKKFVIVVPSVPIREGVLKSLEITKAHFDGVFGRPVMRYAEYSSSRLSDLRTFAV
                     NDHIEILVINIQSFEKDGNVINKVNESGETPIKFIQETCPIVIIDEPQNMETEGRLNA
                     LESLNPLFTLRYSATHKQPYHKVYSLNPVQAYNLKLVKQIEVLPVLAENDVNGAFVEL
                     LEVKQAAKGRPKADLNIHYQDKKETKKKKVSVKWEDDLFEKSGGNEAYRHGFIIDGMD
                     FDKQELAFSSGKTITRGGDGDAVRDEIMKNQIRETVREHLAKEKRLNPLGIKVLSLFF
                     IDRVENYRTEDGTAGKFALWFEEIYRELAGNTDTAGVHDGYFSQDRKGRLKNTDGTTQ
                     ADNDTYRLIMRDKETLLSFDSPLRFIFSHSALKEGWDNPNVFQICTLNETRSPLKKRQ
                     EIGRGLRIAVNQEGRRVRDENVNILTVVPNESYELFAANLQKEYEDECGIKFAAENIK
                     NGNKKKKQLFRKDFPLDPEFQAIWDKLKYKTRYRVRFDTEELIKQASEAVANLPEIQK
                     PKIRIRKAQLKQSQTYGIETVEIAGRSREMDVQWEIPDILGEIQRKTGLTRRTVYEII
                     IQSGRLKDVAGNPQRFIDLTAEAVNRSLHALMAAGIEYERTGEVYDQKLLEDMAREGI
                     EFYENGFTFTVSNPQKTICENYIPLDSGTEKAFAQNCEDYDAKNEGGVAFYFKLPGWF
                     KIPTPLGNYNPDWAVVKQVRDKVYFVAETKNTGKGIQTGVDMDKLRQNERLKIKCGKR
                     HFEALGGLAYRVVEKVGDLKSEKEEQA"
     gene            complement(29348..30517)
                     /locus_tag="ACFM4S_000093"
     CDS             complement(29348..30517)
                     /locus_tag="ACFM4S_000093"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6049860.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLVGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKTTEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTLAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            30665..32191
                     /gene="ilvA"
                     /locus_tag="ACFM4S_000094"
     CDS             30665..32191
                     /gene="ilvA"
                     /locus_tag="ACFM4S_000094"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6049861.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(32248..33321)
                     /locus_tag="ACFM4S_000095"
     CDS             complement(32248..33321)
                     /locus_tag="ACFM4S_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6049862.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(33318..34178)
                     /gene="cysW"
                     /locus_tag="ACFM4S_000096"
     CDS             complement(33318..34178)
                     /gene="cysW"
                     /locus_tag="ACFM4S_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6049863.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIVLATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(34430..35266)
                     /gene="cysT"
                     /locus_tag="ACFM4S_000097"
     CDS             complement(34430..35266)
                     /gene="cysT"
                     /locus_tag="ACFM4S_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6049864.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMLWALGRRSGA
                     KG"
     gene            35405..35512
                     /locus_tag="ACFM4S_000098"
                     /pseudo
     CDS             35405..35512
                     /locus_tag="ACFM4S_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            35557..35889
                     /locus_tag="ACFM4S_000099"
     CDS             35557..35889
                     /locus_tag="ACFM4S_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049865.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GEACACGH"
     gene            36220..36729
                     /locus_tag="ACFM4S_000100"
     CDS             36220..36729
                     /locus_tag="ACFM4S_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6049866.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         9156 a         8582 c         9672 g         9338 t
ORIGIN      
        1 cggtattttc ttaccgtatt ctgcgattgc ctgtcggaaa atgccgatca acctgcctat
       61 aacggcattt tcgccaaatt cgttcagaca gttttcccta agtcgggcag gttcaaaatc
      121 agagtggtgt tcatacattt tgatgagtgc gtcggcaagg gcatcgtcgt cgtcaacagg
      181 aaccaaatat ccgttgccgt ctgaaacaat agattccgca ccgccgcagc gtgttgcaat
      241 gacgggcaat ccttgggaca gtgcttcgat atagaccacg ccgaaggttt cggtgcggct
      301 ggcaaggacg aatgcgtcgc tgttcctcat caaatccaag actgcttcgg gctgcaatgc
      361 gcccaaaaat gtaacggcat gggtaatgcc caagtctgcc gcctgctgtt tcagccgctg
      421 ttcttcctgt ccgctgccgc cgatgtttag acgcagttgc gggcattgtg ccaacgcccg
      481 ggcaaaggca gtgagcagga catcgtgtcc tttgagacgg cgaaggtgcg agacggtgca
      541 gaacacgaaa tgcggtttgt tgtttttttc tctttgttca aacggtcggt tgaatattcc
      601 gcccagtatg ttggggaggt actgccattc gcagccgtat ttgtgttgca ggacgtgtgc
      661 gaaatggcgg ctgatggcga gacgtgcggc ggcgtgcgcc gccgcatttt tcataggctg
      721 ccattggtgg ggacggatca gaccgcgcgt gatggtgctg ctgtgtttcg tgacgacata
      781 ggggatgccg tatttttggg aaatcttgaa ggcaagtatg ccggcatagt tcatacagtg
      841 ggcgtgaatc aggtcgggca gcccgttttc gcggatgtag tgtttgaaag ctttcaaacc
      901 cgcacacacc cagcggatgc agtcgatgtc gatgaacgga aagcggggga agaaatatat
      961 gccgtgccat gcatagatgt ccaaaccgct ttgccgatat tttgcaaaac cgtaaggccg
     1021 gtcaggatgc ttgctgtttc tttccgcaag tagcggaaca tcggtgcaag cacggcggtt
     1081 ttgatgcctt tcctctgcaa tgccagtgcc tgattttgaa aaaaatcccg tccacatcct
     1141 gttcggattg cggataccat gaggggatga cgaggacgtg caacggttcg gacataatat
     1201 gattccgtat cggaaagacg gttattataa gacagacgca gaccgaatat ttaaattgtt
     1261 gccttacgct aatgcaattt agcgcgccga tatgctagat tggcggtttt atgagtgagg
     1321 atacggatat gttgcgttct attttggcgg cttccctatt ggcggtatct tttccggcag
     1381 cggctgaagc attgaattac aatattgtcg aattttccga atcggcgggt gtcgaggtgg
     1441 ctcaggatac aatgtccgca cgtttccaag tggcggcgga aggacgggac aaaaatgccg
     1501 tcaatgccga gtttgttaaa aaattcaaca gttttaacag aaaattgaaa aacggcagct
     1561 ttaaaaccga attggtatcg cgcagtgcga tgccgcgcta tcaatatacc aacggcagac
     1621 gcattcaaac aggctgggag gagcgtgcgg aatttaaggt cgaaggtagg gattttgatg
     1681 cgttgaaccg ttttattgct gatgttcaga cggatgcttc gcttgaatat accgatttca
     1741 gcgtgtcgcg tgaacgccga aacgaggtca tcgatcaggt cagcaaggat gctattttgc
     1801 gttttaagac gcgtgccgaa aaactggcgg gcattttggg tgcgtccggt tataaaatcg
     1861 tcaaattaaa tttgggacac atcggcagcc atatcgcggg agggggagct gctcaggcaa
     1921 aaatgcttcg tgccatgccg atggcggcaa gcgtcaatat ggagggtgcg gattctgccg
     1981 cgcagggtgt ggaggaaatc agcatcagcg tcaatgggac ggttcagttc taaccacgga
     2041 taaacaggta gatgccgtct gaaaccggat gatacggttc agacggcatt tatatttcag
     2101 gctttaggca gggtaacgcc ggtttgcccc atatatttgc cgttgcggtc tttgtatgag
     2161 gttttgcaga tttcgtcgct ctcgaagaag aggacttgcg ccacgccttc gccggcgtag
     2221 attttggcgg gcaggggggt ggtgttggaa aactcgaggg taacgtagcc ttcccattcc
     2281 ggctcgaacg gggtaacgtt gacgatgatg ccgcagcggg cgtaggtgga tttgcccaag
     2341 cagacggtca ggacgttgcg cgggatgcgg aaatattcga ccgtgcgcgc cagtgcgaag
     2401 gaattgggcg ggatgatgca gcagtcgtct tcaacggtaa cgaagttttt cgggtcgaag
     2461 tttttgggat cgacgatggt gctgttgatg ttggtgaaga ttttaaattc gtttgcgcag
     2521 cggatgtcgt agccgtagct ggacgtaccg taggagatga tgcgtttgcc gtcggcttct
     2581 ttgatttggt tcggctcgaa agggtcgatc atgccgaatt cttcgctcat tcggcgtatc
     2641 catttgtcgg acttgatgct cataatgttt tccttgtttc ttgcggtgtt cggacaaagc
     2701 attcgggaat gccgtctgaa aacggggctt atttgttttt gggcagtttc acttcttgaa
     2761 tcatgccgtt ttcacatttc ataatgaaga gggtttcgcc gttttgcgag acggtaaacc
     2821 tgccgtgtgc caggcctttt ttgaacgtgc ccgagagtac catgttgcgg aatttggtac
     2881 tgtcggaatt gaacggttcg ataaatattt cgcggttggc ggaaacggta taaacgcctt
     2941 gtccgtcgaa tttgccattt ttaaacgaac cggtataacc gcgcccgtcc cggcaacgcc
     3001 atatgccttt gcctgaagga agcccgtctt ttccgactcc gccgtcgtag gtgcagccgg
     3061 gttcttgata ggaagtcaga acggcggccg aagtggggag ggcgaacatc atggcgggca
     3121 gtaggaatgc gagatgttta agcataaggg ttattccatt ggattttggt tgacggtatt
     3181 ttgtcgtgaa aaagccgtct gaaaaatcaa tcttgccagc cgcccaaata ggaaaccagt
     3241 tcttccaaca tggtgctgac ggattccgcc atcagaattt gcgaggcgaa ggcaaggccg
     3301 gcggcatcgt cgccgttgct ttcggcttct tcctgcaata cgtcgaggta ttggatgcgc
     3361 ttgagtgtga agtcttgagt gaggataaag gcaatttgtt cgcgccacac caagcccagt
     3421 tgggtaacgg ttttaccgtt tttgacgtgt tgaaccactt cgtcggcggt taaatcttgt
     3481 ttggatactt tgacgacggg aacaatatcg cccgtacctt tgagttcgca atcgctgtct
     3541 aattcaaaac cgccttcgca atgcccttgc aacagccagc cggtcatcaa ggaagagggc
     3601 gattgcttgg tattcggcag cgaggcttcc aaaccgccca aagcttcgcg cagcttggtc
     3661 aggatgtttt ctgctttggc ggaagccgcg ttattgacga gcaggtagcc gtggcgggtg
     3721 ttaaacaccg cttctgtacg gctgctgcgg gtaaacgctc ggggcagcag gtcgtctgta
     3781 atttgctctt taagctcttg tttttcttta cggccgacat tgcgggcttc attgttttgg
     3841 atttccgcta ccttctcttc caaaatatcg cggatgacgc cggcaggcag gactttttct
     3901 tcttttttca gggcgacgcg caaggtaaag tcggcaggga aaacgagttc gggagagaac
     3961 gaaaccggtg cggcaaagcc ttcgctgaac cagtctaagc cttggcaatg ggtaaattca
     4021 gcttcagcaa gtttgtcggc aagtacgtct gcctcaggca gcttttcttt gttgagcgga
     4081 taaaaactaa tctgcttgaa ccacataatg tttcctgttg tttgaaatgt cgggaattat
     4141 ttgctgaatt gttttttcac actgactttg gttttcttct tgaagcggtt tttctcttct
     4201 ttgcggtctt cgcgtttctc aatacggttg ctcaggctga cacggcgcag cggttttttc
     4261 ttcggtttgt cttccgcatt ttcatagggg ttttctgaaa cattgtattg aattctgagc
     4321 ggcgtgcctt gcagattgaa ggctttgcgg aatgtttggg tcaggtagcg cgtatagctg
     4381 tcggaaatcg cgtgcagcga attgccgtgt accacaatta cgggagggtt catgccgcct
     4441 tggtgggcat aacgcatttt cggacgcacc aagccggcac gcggcggttg ctgacgctcg
     4501 attgcgcttt gcaggacgcg cgtgattttc ggcgtcggca tcttaatcat cgccgcgttg
     4561 taggcagcct gaatgctgtc aaacaaaccg tctataccgc gctctttcaa tgcggaaata
     4621 aagtggaact tggcaaaatc gaggaaatac agtttgcggt tgatatcgcg tttcacttgc
     4681 tcgcgacgtt cttcgctgat gccgtcccat ttattgaccg ccaccaccaa agcacggcct
     4741 gcctccaaag cgaaacctgc aatcgtcgca tcttggtcgg cgatgtcctg ctgcgcgtcc
     4801 aataccaaaa cagcgacgtt tgccgcttca accgcctgca tcgctttgat aacggagaat
     4861 ttttccactg cttcatccac tttgccgcga cggcgcacac ctgcggtatc gatgatggta
     4921 aacggtttgc cttcgcgctc gaaatcgata tggatactgt cgcgcgtcgt gccggccata
     4981 tcgaaggcaa tcacgcgctc ttcgccgaga atggcgttta ccagcgtgga tttgccgacg
     5041 ttcggacgac cgatgacggc aaaaacggga tgttttgcat cggcttcttc gacttccggc
     5101 tcggggaagt tttctaaaat ttcttcaatc aggtaataca cgccgtcgcc gtgcgcaccg
     5161 gaaataacat aagggtcgcc caaagcaagt tcgtagaact cggcggcaag cacggccctg
     5221 ttgccgcctt cgcctttgtt taccgccaga taaacagggc gcgggctttg gcgcaaacgg
     5281 tcggcaatga ttttgtcttg cggggtcaga ccggtgcggc cgtccaccaa aaatacgacg
     5341 gcatcggctt catcgacagc ctgcaaggtt tgttttgcca tttcgtgcaa aatgccgctg
     5401 tccacaaccg gctcgaaacc tccggtatcg atgaccaaat aaggtttgct gccgactttg
     5461 ccgtgtccgt aatggcggtc gcgcgtcaga ccgggcaggt cgtgtacgag cgcgtctttg
     5521 gtgcgcgtca aacggttgaa taaagtagat ttgccgacgt tggggcggcc gacaagcgcg
     5581 atggttggtt tcatgattgg gtctttctgt gtcaagtgcc gttcgggaga actgaacaca
     5641 agcaggtgtc cgttgggaca cggcagatgg ttttacggga attgccgtag gatagtgttg
     5701 tctgaaatgc cgtctgaaga gagggtggca tttcagacgg catttatttc agcgaatcga
     5761 gtttcatttg aaccaattca cgaccgacag aatcttgagg cattttttct aaagcctgac
     5821 cgtagttttt taaggcttcc tggctttttc cctgtgcggc atagacatcg cctttggttt
     5881 ccatcagcag gggggagaag tccgcttcaa ccggcgtgtc gagcgcggca agcgcggcat
     5941 cgtatttttt ttgttgcaac aacacaacgc ccagacgctg cgccgccaac gcttgaatca
     6001 ggctgtcttt ttggttggac aacacccatt tcaaatggcc ttcggcaaca tcgtaacgct
     6061 gcgcgtcaaa ttcggttgcc gccgccatca gcgtggcttg ggcggcggaa atagaatgcg
     6121 ggtagctttg ttggagtttg gtcaattcgg cattaatttc gctttgcggg gctttgtttt
     6181 gcgccttttc cacgatgttt gccagcaccg ccgccgcttc ctgattttgg gaagccgcac
     6241 ggttttggta aaccgtgtat cccaagtagc cgagtgctgc caaaatcagc agggcaaaca
     6301 gccatttgcc cgtggttttc caaaaatatt taaagttgtc taactcttgt tgttcttcga
     6361 gatgggctgc catttatgcg ttcttccatt gttgtaaagt atgggttaaa tcctcggcgg
     6421 cgacagtttg ctgaccgtgt gcgccgttca tgtctttgag cgtaaccgta ccgttcgcca
     6481 gttcgtcttg cgcgacaatc agggcaaagc gcgcgccgct gttgtcggct tttttcattt
     6541 gcgctttcag gctttgatag ccggaatgct gcattacatt gaaaccttgc gcgcgcaagg
     6601 cttgcgcgta tttcatcacc tgcaagtccg ccctttcgcc ttggtgcatt gcatagacat
     6661 caggcgcagc gttcacttcc agcgagccgt attcgctcac caaaagcagc agccgttcga
     6721 tgcccatcgc aaagccgatg gacggcgcag gtttgccgcc aagttcctca atcaaaccat
     6781 cgtaacggcc gccgccgcac acagttgcct gcgcgccgag tttgtcggtc gtccactcaa
     6841 aaaccgtctg attgtaataa tccaaaccgc gaaccaagcg cggattttca atatattgaa
     6901 tacccaaacc atccaacatc gccttgaagc gtgcatagtg gttttgcgaa gcctcgccca
     6961 agtaatccac caaacgcggc gcggcgttgc agatttcctg caaatcaggg tttttcgtat
     7021 ctaaaacgcg caaaggattg gttttcagac ggcgtttgct gtcttcatcc aatttatctt
     7081 cataacgggt cagatattca accaatgccg cacggtgtgc cgcgcgttcc tcacggttgc
     7141 ccaagctgtt gatttccaaa gtcaggtatt cgcgaatacc caatttttcc cacaagtcgg
     7201 cagacatcgc gatgatttcc gcatcaatat ccggaccttc aaaacccaaa gcttcgatac
     7261 cgacctgatg gaactgacga taacggcctt tttgcggacg ctcgcggcgg aacatcggcc
     7321 ccatatacca cagcttttgc gggctgttgt agagaaggtt gtgttcgacc accgcacgca
     7381 gacaagaagc cgtaccttca ggacgcaggc tcaaactcaa agaatcgttt gaatcggaaa
     7441 aggtgtacat ttccttgccg accacatcgg tttcttcgcc gatggagcgg acaaacaaac
     7501 cggtttgctc gacaatcggc gtacggattt gctggtagcc gtaagcgcgc gtccaacggt
     7561 ttaccgtatc ttcaaacacc tgccaaaacg cagccgtcag tttgaaatct ttttgttcga
     7621 caggtaaaag gtcgttcata ccttttacag attgaatttt ttgtgccatt tcaatatggg
     7681 ataagcgaaa atcaaatagt tgggcattat agctgatttt taagggcttg tgaggataga
     7741 gatggtttgc ggacgggtaa aaatgccgtc tgaaacagtt ttcagacggc atttgactta
     7801 ctctgcacgt gcttgcctga tttgtccgac tgcaaactct gtctgccgct tgggatgatg
     7861 tgtgaaaaac aatttatttg aaattgtctc ggcttttttc ggcacgaccg ccaaaatctt
     7921 acctgccaaa tttccctcac gggtttgcca agcatccaaa aactgcgccc tgctcattga
     7981 aacatgcccc agcgacgggt cggcaagcaa aaccgtattg ccgtctatcc cgcgcaatac
     8041 cgagaaatga tcatccttgc ggtatttcag atacacgatg acggggattt tcaactgtgc
     8101 gagctgctcg aaagacaggg catagccttt cgcttcaaaa cccaaatctg gcataatgcg
     8161 ccgcatatcc tcaaacgacg cgcgcatctg ctccttatcc agctttttca acacttcttc
     8221 ttccgtcagc gtttgcccgt aaaaattgtt caaaagcgtc gccaccgaag ccgccccaca
     8281 ggaaaaatcc aaatcctgct ttacaatatt gaaatcccgc cgcgctttcc aactctgcac
     8341 tttgattttt ccgtaaacaa caggattatc gttaaacatc ggtgcagcat tcaaacgata
     8401 agataaagaa acgacaacac acgccaacag aaaaacatat ttgaacttca tcatattgtc
     8461 cacataaagg gcagcctgaa aatctttcag gctgcccttg tcaaattatt cctagctttc
     8521 ggcttttttg gcaaaccaaa caatccgatt acccgcataa tactttccat ttattgaaat
     8581 ccaacaagcc gcgtccaaaa aatgccatgc actgccgatt tccgcagcaa tctttgtacc
     8641 gttttcttca aattccaaat attcacccaa taataaactt gaaacagaac gcgtgggcag
     8701 caagtgccca ccctacgcct attcaaataa tttgattaaa taaagaataa agaaaatgca
     8761 tcaggaacaa aattataatc tgccacctga ctcacaccgc ttttaaagta aggggcatca
     8821 aaatcaaaac cgcaaaaaaa ataatttttg cattgatttt taatagattt aaaattcgaa
     8881 tatagtgttt ctccagattt aaaaagttta tctttatcta tatatttgat gctttcccta
     8941 tccaaaataa atatttcaaa cattaaaaaa tcattacatg accaagccaa atcataaatt
     9001 tgattcaaat gggtatcata aagataaaaa taataaggtt tgggaacagg taaaatattt
     9061 aatggaaaag gaggactaat tttcttaata tctgatgact gatatccata tctttctata
     9121 ttcttaaaaa tttcatcttt cttaagataa caagacattc tgacaatcac aacctgttca
     9181 tcggatatat tatctatttg catggcgcgt ataacacgcc atgcctgatt aaaattagtc
     9241 tcccttacct tagaatctat tatctttttc aaagatgaaa atgcccctta tcttgaactc
     9301 tccagttaga acctggaagt ttcgtacctc ttaaatgtgt attaatattt cttatagttt
     9361 cattaaaatg ccacgcgctg ccgatttcaa cggtaatttt cgtaccgctt tcttcaaatt
     9421 ccaggtattc ccccattagc taacgcaaag aagcagacgc catttcggct tcgttatgat
     9481 aaacccgcct tccgttgata tagacttccg cccctgtccg ctccaaattc caaaaattcc
     9541 gtactgaaat ttccatatcc cgatattgtg cagaccatgt tttttcgaag gttttcataa
     9601 aatttcctat acctgtccaa tcggcacata tcaattgcat tattatagtg gattaacaaa
     9661 aatcaggaca aggcaacgaa gccgcagaca gtacaaatag tacagaaccg attcacttgg
     9721 tgtttcagca ccttagagaa tcgttctctt tgagctaagg tgaggcaacg ccgtactggt
     9781 ttaaatttaa tccgctatac atctcaatac gataaatatt ccttaagtca aaatgcaagc
     9841 ctgaccgtac cttaactgtc aaaattttat tattttttat tgattttaaa acaatttctg
     9901 caaaattctc ttcgctttct ctctttttta gaagcacata agaaaaaata aaacttcccc
     9961 gattaaattc ataaatatgt ttcaaccatt cgcctcctct ttctgtaaga caagattcag
    10021 tttcattctt ccttattgta taaatatttc cttcacaaaa tctgaaataa atccataaat
    10081 ccatcttatc cataattaaa gaaaaagttt cacctcgaga ttttgtcaac aattcgcaag
    10141 gttgcgatgt tgcaatcaaa tagccgaaag acatttttta cctcatacat ggtcgaaatc
    10201 agtttctgtt agttcagaat ccattttttc gtcaacaact gaatccgcat ttttgaatta
    10261 acgttttcat cagctgccgt ttatctaaac cggcaggttc agtttcaaaa taagccttat
    10321 atgaagactg taagcatttc agaaaaagat catcagaaga catatctgcc gaatcaaata
    10381 caactgtttt gattttggta cttacccaaa accctttttg ctctttttct actatacgga
    10441 aattcagaat atttccaacc gaatcaaaag cacggtaaac atcatccatc aaatcctgcg
    10501 gctctatttt cttttccaat tccgacaatc cttgaaatat atccaaagac acatcttcaa
    10561 atagaaaaaa aggaggagtt agaagcggtt tttccatgat ctgtccgtag attttgattc
    10621 ccaagggcga tgacgaccaa ttccctgtcc aggcaaagtc ttgcccgtat tatccgtaac
    10681 tcgacgatga taatggggaa attttccaat aggatgacct gttctattac cgaaaggggc
    10741 tatccgcata ttattgccga ttttaatctc acgtccatat ttagcaaagg aaacaacctt
    10801 tcctgcggcg cctacaccac caggaattgc gcctaatccg ccagcaatag caacatctct
    10861 aacagaagct ggtctgcctg tcgttgcata actaaaacca tgctgtgtcc acataccaat
    10921 ggcagcacca cccaagatag ccaatggaag aaacgccccc tctgtctcct tcatctcctt
    10981 ttgagaaagc tccgccaact gcatcggtgc atctgcccgc gtgtggaaca cttggtcttc
    11041 aaatgcctga ttgtccaagc cgtttgccat tgcgggggca atcatagaca gcatcattac
    11101 ggctgcggtg atttgttttt tcataataac tcctttggat tacaaggttg gaaaatcaaa
    11161 gccctgctta gaacgtatgt tgcacaccca atttcaatag gtaaatcaga ttgcaaatcc
    11221 agcaatttga atattgtcat tgttccgtgc aaaagggatt tttattgatg agttgtgtac
    11281 tgggtttcag cttggctttt tagataatct tttcacaaga acatacatca atactgtcca
    11341 aaaaaaaaag atttgataaa tatattgcaa catattttat tccacctcca tccgaaacag
    11401 aaaccaatat atttttaatt aatacatagc tgattattac atttattatt cctataatat
    11461 ataaggatct tgccaaaatt ttttttgatt ttattttagg aatatctctt atccatgcta
    11521 aaatacagcc caatgtcgaa aagaaaataa cagcaaacac taccttacct cctctcactt
    11581 ccaaccaaac cgatagcagg tttggttgga agttggttta tttattattt aacggcgaat
    11641 gtcagtgttc ttacccgtag aacctgcata accatttatg ccaccagcaa cagtccccac
    11701 tgctacacct cgcataccat taatcagaat tcttcctatt ttttcgaatc cactatttct
    11761 tccaaagatg cagcaaacgc aggttgagcc tagacccaat ctacgtttgc tgccgcatct
    11821 gatccctatt gtttcttatc cttacatctt cctgccttgt caatcaaata aagacagaag
    11881 actatacaaa aactgaccga catactgaaa atacctatcc ccttccatgc aatctccgca
    11941 ccattgaccc aatagattag actgagaaac aacaaagcca cagtataaat caacgtaaaa
    12001 aatattgcgt aaagcacaat aaagggaact tttatctcac ggttgttttt tataatatat
    12061 tcagcaactt gatttccgaa tatacctgat aaaaaatata gtattagatc atccatttcg
    12121 tttatcttct atgttttccc attgcggcgc tagaagactg atttaatgct gcaccatttg
    12181 cacgaataac tgcattagga atagcatttc cccctttcca agcagaacgg gcaaaaacac
    12241 tagtagtaat ccctgcaccg cgcaacatcg cactgctata tccgccgcct atcataccac
    12301 cagcggttgc agccaaggta cttcttgttg aagcaaattg ccctgtttta attttagaaa
    12361 tgccgtgatt agcccaagca ctaattgccc ctcccatcaa agcacctgcc acaataggaa
    12421 gaaacgcccc ctcagtctcc ttcatctcct tttgagaaag ctccgccaac tgcatcggcg
    12481 catctgcctg cgtgtggaac acttggtttt caaatgtctg attgtccaag ccgtttgcca
    12541 ttgcgggggc aatcatagac agcatcatta cggctgcggt gatttgtttt ttcataacaa
    12601 ctcctttgga ttacaaggtt ggaaaatcaa aacctgctta aaatgtatgc tgtacgccca
    12661 atttcagttc ggaactgctt tgccctgaaa cgttgaaacg tgcggatgcg tttaaagccg
    12721 tggttttggt gaaaccgaaa cctgcgccga aatgggcgta ggtggatgtg tttcttgcgg
    12781 attctttttt gccgtctatg cggtcgggct gtttgcccag ccattggatg cctccggtca
    12841 ggctgattct gtcgttggcg gcaaatgaga tgttggggtt gagcagcagg tagttgcccg
    12901 atttgtagcg gatgccgtct gaaagggttt tgctgccgtt gatgcggtag gcggcggtga
    12961 gggaaaggac aatcggatct atggctttgt aggtggtggc gccgatgagc cacgattttc
    13021 ccgacgaggc tttgttgcgc gatttttcgt aaaccgtgct ttcaagaaag ctgattaggg
    13081 cggggttttt gtcgtcttta aggaaggtgt ggctgatgcc gagggatacg tcggacatcc
    13141 gtttgttgcg ggttttgctg ttgccgtcga gtttgcgttc ttcgtgccac agatagctgc
    13201 cgctgccgta aatgtcggta ttcccggtca gtccgtagcg caaaccgagc gtgccaacga
    13261 gcatatcggt attgctgccg ttttcttgga tttcggtcgg aatggggata aacgaggttg
    13321 cgccggtttg gatgtaaacc agtgcggcaa gttcggcgcg gttgttttcg ctattcaggt
    13381 aggtaaggga agtttccagt ttccatttgc ccttgtcagt cattatgtct tcaatcgtca
    13441 agggcaggtc ggcataagcg gataaaggca ggatggcggg caaggcgggc aaaaagatgc
    13501 gcttcatatt tctcctattg atattaattc ttatttaaga atagtaatga atatccgata
    13561 attttgtcta aataaatttt taaatcaaaa aaaaacgata tatttgtttt tatcaattat
    13621 gttttgatat gccgtctgaa aagtttgtga aaaacggtta caatccgccg catgaaaaaa
    13681 cgcaataatc ctcttccgtt gttgaacggt gtcaaaccca gttatttggt gctgccgcat
    13741 gaaaagcagt tttacgggct gccgctgctg cattttctgt gcatccgctt tccttttgtg
    13801 ggcgcggacg attggcgcag gcggttgaac agcggttttg tggtcggttc ggatggtgcg
    13861 gcgttggacg aacatacttt gttcgagccg ggtaaggtga tgttttatta tcgggaaacc
    13921 agcagtgagg atgagccgcg tattccgttt gaagaaaaga ttttgcatat tgatgagcat
    13981 ttgattgtgg tggacaagcc gcattttctg cccgtcatcc ccagcgggcg ttttttaagg
    14041 gaaacgctgc tcacacgtct gcgcctgcgt cccgaattgc agcatttgaa tgttgaagac
    14101 attacgccgc tgcaccgctt ggacaaggat acggcaggcg tgatgctgct gtcgcataat
    14161 cctgccacgc gcggagccta tcagacgatg tttcaaaaca aaacggtatg gaaaacgtat
    14221 gaggcgcttg cgccgacaag gacggatttg ccgtatccgc tcgatgtggt ttcgcgtttg
    14281 gtgaggggtg agaaattttt tacgacgcaa gaggcggaag gcgaaccaaa cgcacatacg
    14341 acggtcgaac tgattgaaaa caggggggaa ttcagccttt accgccttac gccgcatacg
    14401 ggcaagaaac accagttgcg cgtgcatatg atgggtttgg gtatgccgct gttgaatgac
    14461 gcgctctatc ccgtgccgtc tgaagcgggc agcgaggatt atcggaaacc tttgaagcta
    14521 ttggcaaaaa agattgcgtt tgcagatcct ttgtcgggtc gggaaagggt gttttgcagc
    14581 ggattttgcc tataatgaga gggaacgcaa acccgtatcg gcggatgccg ccgatacggg
    14641 ttttacatta tcgatgccgt ctgaaagggg ttttatccgt tcaggcatac gggcgcgaag
    14701 cggaggatgg tgtcgcgcag gacgatgagt ttgccgtgaa agaaatgcgt ggaaccggcg
    14761 atggtaatga cgggcaaatc ttgcggttcc gcccatttca gtgctttccc gatttcgacg
    14821 acttcgtctt ccgcgccgtg tatcatcagc gttttggcaa cattgggaac ggcggacggc
    14881 tcggggcggc cggtatagtg gcagactgcc gcgccgatga gcagcaataa atcgggtatg
    14941 cgcgcttgtg cggcaaatgt ggcgacataa ccgccgaagg agaagccgga taaggcaaat
    15001 tcgggggctt cgggatgttg ggcacgagca tagtcgatga cggcgataca gtcttgcgtt
    15061 tcaccgtgtc cgtaatcgtg tgtgccttcg ctgccgccta cgccgcgaag gttgggcagg
    15121 tagcagtgga agccgagttt gcttaaggct ttggcggcgg tttggatgac tttgttggtg
    15181 ttcgtcccgc cttggagggg gttggggtgg ttgatgacgg caacaccgcg tgccggtact
    15241 tgttcggacg ggatatggat ggtttctaaa atgccggcag ggccgggtat gtgtatggtt
    15301 tcaggttttt gcataatggt ttcctcttga ttttccgcat attccgatgc agccgcgaag
    15361 atggcggctt aaaggtggaa atgcttgccg ttgttggggt tggatgcttt caggtcggcg
    15421 agcatgcggt ggaagtccaa tccgtaagcg ttgctcagtt tgtcggcacg ttttttgagt
    15481 ttgtctatgt tttgctcttt ggggctgctt tggaaggcca ttaccgcgac attgccgtgg
    15541 ctttcggcag gaagttccaa gacgcgccct tcaaaaacgc tcaacaaccg ttcgatgaag
    15601 cgttggtagc gtttgtcgcc gctccaccag ttggttacga atatgccgtc tgaagagagt
    15661 gcgtttcggc agtctcggaa aaacggttct tcaaccagcg catcgatgat ttgttcgccg
    15721 tcgaacccgt ccaccaaaat cacatcggtg ttgtggcgga agactttgat atattctgca
    15781 ccgtctgctt cgataatttc aaatttctcg ccctcgaaag gcaactcgaa caggctgcgg
    15841 gcaatggcga tgacctgcgg gttgatgtcc acggcggttt ggcgcgtgtc gggcaggtag
    15901 gtgtctatcc agcgtgcaaa cgaaccgccg cccaagccga tttgggtgat atgctgcgga
    15961 agagcgtcgg taaacagcag ccatcccatc atggcacggc tgtaagagag taccagctcg
    16021 gacgggtggt cgaggttcat tgagctttgg atggtgtcgc tgcccaagtg aagcgaacgg
    16081 atattgcctt cttcggaaat gccgacttcg ggaaagctgg attttgcagg acgcaggcgg
    16141 cggtagggat gtcttgccat cggtgtgaac ggtcggtttg aaaggcagat attttatcag
    16201 aatgccgatg gttgtcctgc ttcaaattaa tttcttttca aataaattac ttattcggat
    16261 ttgccggggc tttcggataa attccttgcc aaggtgcggc attgcctgca taattcgctt
    16321 tctttgccgg tatagctcag ttggtagagc acctgacttg taatcagggg gtcccgagtt
    16381 cgactcttgg tgccggcacc atttctgctg atggtgttgc aaaacatttc agtaagccgg
    16441 tatagctcag ttggtagagc acctgacttg taatcagggg gtcccgagtt cgactcttgg
    16501 tgccggcacc agatttgaca gtccgattgt gtaaaacagt cgggctgttt tgcatttgcg
    16561 gcacggtcgg tggcagggct gcgggcattt cactataata gcccgtttca aactgaaaag
    16621 gccgtctgaa aagggcgggg taacaatatc tgatgattac tgtgaacaca ctgcaaaaaa
    16681 tgaaggcggc gggcgaaaaa atcgctatgc tgaccgctta cgaatccagt tttgccgcgc
    16741 tgatggacga tgccggcgtg gaaatgctgc tggtcggcga ttctttgggg atggcggttc
    16801 aggggcggaa atcgacgctg cccgtcagcc tgcgcgatat gtgttatcac accgaatgtg
    16861 tagcacgcgg tgcaaaaaat gcgatgattg tcagcgattt gccgtttggt gcatatcagc
    16921 agagtaagga gcaggcgttt gccgccgctg ccgaactgat ggctgccggc gcgcatatgg
    16981 ttaaactcga aggcggcgtg tggatggcgg aaacgactga atttttgcaa atgcgcggta
    17041 ttccggtttg tgcgcacatc ggtctgaccc cgcaatccgt gtttgctttc ggcggatata
    17101 aagttcaggg gcgcggcggc aaggcgcagg cgttgcttaa cgatgccaag gcgcatgacg
    17161 atgcgggggc ggcggtcgtg ctgatggagt gcgtactggc ggaactggca aaaaaggtaa
    17221 ctgaaactgt ttcctgtccg actatcggta tcggtgcggg tgtggattgc gacgggcagg
    17281 ttttggtgat gcacgatatg ctcggcattt tcccgggtaa gacggcgaaa ttcgtcaaaa
    17341 actttatgca ggggcatgat agtgttcaag cggcggttcg ggcgtatgtt gccgaagtca
    17401 aagccaaaac cttccctgct gcggaacata tttttgcaga ttgaacggtt tacatgcagc
    17461 atgccgtctg aaagccgttt cagacggcat tttgttttgc cgtgcgcggc gcgtataatc
    17521 ggcgcgtttt gtcgggcagg aagcccgaaa gataaggatt accgttatgc aaatcataca
    17581 taccattcga gaactgcgcg cgtggcgtaa aaatgcggga aaggtggcat ttgtgccgac
    17641 catgggcaat ctgcatgaag ggcatcttgc gcttgtgcgt gaggcgaaaa aacgcgcgga
    17701 cagtgtcgtg gtcagcattt tcgtcaaccg cctgcaattc ggtcagggcg aggatttcga
    17761 caaatatccg cgtactttgc aacaggatgc ggacaagctg gaagccgagg gtgttgccgt
    17821 tgttttcgcg cccgatgaga aagaactcta tccgaacgtg gaacagcgtt acaacgtcga
    17881 accgcccaat ctgcaaaatg agttgtgcgg caagtttcgt ccgggacatt tccgcggggt
    17941 ggcgacggtg gtgtccaagc tgttcaatat tgtcgcgccg gatgttgcct gtttcggcaa
    18001 aaaagactat cagcagcttg ccgtgattaa aggtttgacc gaggatttga attttgatgt
    18061 tgaaatagtg cctgtcgata cggggcgcgc ggaagacggt ttggcactct ccagccgcaa
    18121 ccagtatttg agtgcggcgg aacgcgacga agcaccgcgc ctgtactgcg aattaaaggc
    18181 tgttgccgaa tccttggcgc agggcagttt ggattatgca ggtttggagc ggcaggctgc
    18241 cgaccatttg accgctgcgg gctgggtggt cgattatgtc gaaatccgcc gcgccgatac
    18301 gctcgaagtg gcgcgggcgg gagataagaa actggtggtc ttggccgccg cccgtctggg
    18361 gacgacgcgc ctgattgaca atttggaaat aaaactccct taaaccgcaa gcgtcgggaa
    18421 tgccgtctga agcggatttg cgtttcagac agcatttatt tttttgaacg gggtttcgca
    18481 aatctacaaa cgattctgct tgtgataaag ttacgcctga ttatatgccg tctgaaagtt
    18541 cggacggatg tcggataaag gatgattatg ctacctaacc gtttcaaaat gttaactgtg
    18601 ttgacggcag ccttgattgc cggacaggta tctgccgccg gaagcggtgc gggggatatg
    18661 aaacagccga aggaagtcgg aaaggtttta aggaaacatc agcgttacag cgaggaagaa
    18721 atcaaaaacg aacgcgcacg gcttgcggca gtgggcgaac gggttaatca gatatttacg
    18781 ttgctgggag gggaaaccgc cttgcaaaag gggcaggcgg gaacggctct ggcaacctat
    18841 atgctgatgt tggaacgcac aaaatccccc gaagtcgccg aacgcgcctt ggaaatggcc
    18901 gtgtcgctga acgcgtttga acaggctgag atgatttatc agaaatggcg gcagattgag
    18961 cctataccgg gtaaggcgca aaaacgggcg gggtggctgc ggaacgtgct gagggaaaga
    19021 ggaaatcagc atctggacgg actggaagaa gtgctggctc aggcggacga aggacagaac
    19081 cgcagggtgt ttttattgtt ggcacaagcc gccgtgcaac aggacgggtt ggcgcaaaaa
    19141 gcatcgaaag cggttcgccg cgcggcgttg aaatatgaac atctgcccga agcggcggtt
    19201 gccgatgtgg tgttcagcgt acaggggcgt gaaaaggaaa aggcaatcgg agctttgcag
    19261 cgtttggcga agctcgatac ggaaatattg ccccccactt taatgacgtt gcgtctgact
    19321 gcacgcaaat atcccgaaat actcgacggc ttttttgagc agacagacac ccaaaacctt
    19381 tcggccgtct ggcaggaaat ggaaattatg aatctggttt ccctgcacag gctggatgat
    19441 gcctatgcgc gtttgaacgt gctgttggaa cgcaatccga atgcagacct gtatattcag
    19501 gcagcgatat tggcggcaaa ccgaaaagaa ggtgcttccg ttatcgacgg ctacgccgaa
    19561 aaggcatacg gcagggggac ggaggaacag cggagcaggg cggcgctaac ggcggcgatg
    19621 atatatgccg accgcaggga ttacgccaaa gtcaggcagt ggttgaaaaa agtgtccgcg
    19681 ccggaatacc tgttcgacaa aggtgtgctg gcggctgcgg cggctgtcga gttggacggc
    19741 ggcagggcgg ctttgcggca gatcggcagg gtgcggaaac ttcccgaaca gcaggggcgg
    19801 tattttacgg cagacaattt gtccaaaata cagatgctcg ccctgtcgaa gctgcccgac
    19861 aaacgggagg ctttgagggg gttggacaag attatcgaaa aaccgcctgc cggcagtaat
    19921 acagagttac aggcagaggc attggtacag cggtcagttg tttacgatcg gcttggcaag
    19981 cggaaaaaaa tgatttcaga tcttgaaagg gcgttcaggt ttgcacccga taacgctcag
    20041 attatgaata atctgggcta cagcctgctt tccgattcca aacgtttgga cgaaggcttc
    20101 gccctgcttc agacggcata ccaaatcaac ccggacgata ccgctgtcaa cgacagcata
    20161 ggctgggcgt attacctgaa aggcgacgcg gaaagcgcgc tgccgtatct gcggtattcg
    20221 tttgaaaacg accccgagcc cgaagttgcc gcccatttgg gcgaagtgtt gtgggcattg
    20281 ggcgaacgcg atcaggcggt tgacgtatgg acgcaggcgg cacaccttac gggagacaag
    20341 aaaatatggc gggaaacgct caaacgtcac ggcatcgcat tgccccaacc ttcccgaaaa
    20401 cctcggaaat aatgcaggtc catcctttca gacggcataa ggtttgccgg gaagccgggg
    20461 cattcgggca aacggcacgc agttcgcacg cgttattgca cggcacaccg aacccatcgg
    20521 ccggcaggat ggcatccgtt aaggaaattc tgatgaaaca cactgtatcc gcatcggtca
    20581 tcctgctttt gaccgcttgc gcgcaattac ctcaaaataa cgaaaacctg tggcagccgt
    20641 ccgaacacac ccgcagtttc acggcagaag ggcggttggc agtgaaagcg gaagggaaag
    20701 gttcgtatgc aaatttcgat tggacatacc aaccgcccgt ggaaaccatc aatatcaata
    20761 cccctttggg cagtacgctc gggcagttgt gtcaagacag ggacggcgca ttggcagtgg
    20821 acggcaaagg aaatgtctat caggcggaaa gtgcggaaga attaagcagg cagctggtcg
    20881 gtttcaaact gccaatccaa tatctgcata tctgggcaga tggcaggcgt gtggcgggcg
    20941 cgccttaccg catcctgccg gacggcatat tggaacaata cggttggact gtcggcagaa
    21001 ccgccgacag tggggggcaa gtccgaacgt tgcaactgaa taacggaaat ttgaacatca
    21061 ggctggtttt caccgaaatc ggtatgccgt ctgaaactga aaccctggaa cgctgtgcgg
    21121 cgcgcacgag ataaggcgga cagatgaata ttgcggacgg acggcaggcg tttcccgcac
    21181 ctgcaaaact gaatctcgat ttgaggatta ctggaaagcg ggaagacggt tatcacaata
    21241 tcgaaagcat attctgcctg atagatttgc aggataccgt atatttgaaa ctaagggacg
    21301 acggcaaaat catcctgcac aatccggttg atggaatgcc gcaggaagta gatttaagct
    21361 accgtgccgc atcgttgctg caaaaatatg cgcgcaaccc cgccggcgtg gaaatatggc
    21421 tggacaaaaa aatcccgaca ggggcgggtt tgggcggcgg aagctcggat gcggcaaccg
    21481 ttttgctggt gttgaaccgt tggtggcagt gttcccgaac gacgcagcag ctgattgact
    21541 tgggcgcaac tttgggtgcg gacgtaccat tttttatttt cggaaaaaat gcttttgcaa
    21601 gcgggatagg tgaaaaactg ataggaatgg atattccgaa acagtggtat gtcatcgtca
    21661 aaccgcccgt ccacgtttcc actgcaaaaa ttttcacaca cgaaggcttg acacgaaatt
    21721 ccgcctcaag cataatgccg actttccaaa acctgcaacc gtttagaaat gatatgcagg
    21781 cagttgtatt taaagaatac cctgaagttt ggaaagccta ttccgaattg tcccgatatg
    21841 gatttgcctt aatgacaggt tctggtgcgt gtgtattcac ggcgtgtcaa gatagggata
    21901 gcgcatacaa tatataccga caagtttcag atttgtacga ggcatatttg gcagagggtc
    21961 tttcaaaaca tccttggttg tccgtataaa cattgttggg gagtcgtcaa gcggttaaga
    22021 cactggattt tgattccagc atgcgaaggt tcgaatcctt cctccccagc caagtcaaac
    22081 gagttgggga gtcgtcaagc ggttaagaca ctggattttg attccagcat gcgaaggttc
    22141 gaatccttcc tccccagcca gccctttttg gggagtcgtc aagcggttaa gacactggat
    22201 tttgattcca gcatgcgaag gttcgaatcc ttcctcccca gccagataaa agcgtgtaag
    22261 cctgcttaca cgcttttatt tcatagaaat aaaaatattg aaatgccttt gtttgtgtcg
    22321 gatgttgcag gtataatgtc gggcttggta caagcagagg gaagcattgt gttttctgag
    22381 cggaagttaa acataaaatc aggtgagaat atggctgcgt acgacagttt gatggtattt
    22441 acaggcaatg ccaatcccga attggcacaa cgtgttgtca ggcatttgga catttctttg
    22501 ggcaatgctt ccgtatccaa gttttcagac ggcgaagttg ccgtcgaact gttggaaaac
    22561 gtacgcgggc gcgatgtttt catccttcag ccgacctgtg cgccgaccaa tgacaacctg
    22621 atggaaatcc tgacgatggc ggatgcactg aagcgtgctt cggcaggtcg tattaccaca
    22681 gccattccgt atttcggcta tgcgcgccaa gaccgccgtc cgcgttccgt ccgcgttccg
    22741 atttctgcca aactggtggc aaatatgctg tattcggcag ggatcgaccg tgttttgact
    22801 gtcgatttgc atgccgacca gattcaaggt ttcttcgata ttccggtgga caatatttat
    22861 gccaccccga ttctgttgaa cgacatcaaa caacagcgga ttgaaaatct gaccgtcgtc
    22921 agcccggaca tcggcggtgt cgtccgcgcc cgcgccgtgg caaaatcctt gaatgccgac
    22981 ttggcaatca tcgataaacg ccgcccgaaa gccaatgtgg cagaagtcat gaacatcatc
    23041 ggcgatattc aaggcagaac ctgtctgatt gtggacgata tgattgacac cgcgaatacc
    23101 ttgtgtaaag ctgccgtcgc cctgaaagag cggggggcgg aacgtgttct agcctatgcc
    23161 agccacgctg tattctccgg agaggcggtc agccgtatcg cctcatccga aatcgaccag
    23221 gtggtcgtaa ccgataccat tcctttgtct gaagcggcta aaaactgcga ccgtatccgt
    23281 caggtaacga ttgccggtct gttggccgaa accgtccgcc gcattagcaa tgaagaatcc
    23341 gtctcatatc ttttcaatga agaagtgatg acaggcagca tgttgctgcc ataagcccga
    23401 agccgtctta agctggtcgc ggccgatgac ggcgatttta cctaacttgg agtatttaac
    23461 atgacttatg aaattcaagc ttctgttcgt gaagcacaag gcactggtgc gagccgccgc
    23521 ctgcgtcgcg aaggccaaat ccccggcatt ctgtacggtg aaggtcaaga gcctgttgca
    23581 atcgctgtgg atcacaaaac cgtattctac gcattggaaa aagaatcttt ccatactgcg
    23641 ttgattaagt tgtctctgaa cggtgaaacc aaagacgtta tcgtccgcga tttccaaatg
    23701 cacccgttcc gccgcgaagt tcaacacatc gacttccaag ctgtgaaagc cgatcaactt
    23761 gtacgcatcc gtgttcccct gcacatcgtt aacgctgaaa attcccaagc ggtcaaactg
    23821 caaggcggcc gcgtatctct gttaaacact tctgttgaag tagttgcttt gcctgccaac
    23881 atccctgctt tcttggattt ggattgtgcc gaagtggttg ccggcgacat tctgcacttg
    23941 tcagacatca aactgcctga aggtgtagaa agcgtttccc tgaaacgtaa cgaaaatctg
    24001 gctgttgcta ccgttaccgg taagaaacgc taattgattg aaaagcaggc ggtacgtata
    24061 tgatgcgtac cgccttattg tttttggctg tgtttcaata tttggttgat ttcatccgtt
    24121 tataagggaa aaccgcttat ttccgttcgg gtggaaattt atccagacgt attgcgcgac
    24181 aacggatggt tggctcaaaa acggcatttc gtaccggatg aaaatgaaga agatatgtcg
    24241 ttcacattcc ggtagctgtc cggctcgaac cggctatcag agatagtcgg tcaattttca
    24301 ccctgaaaat cacttttctt ttaacttgca acatattccg ataaaatgga cactttgtaa
    24361 gtaatcattg atggaagacg caattatggc aaccgaaccg aaccgaaatt atacacgatt
    24421 tagaagataa tttcaaacaa aacaatatat taaaactcaa acaactcttt cccgaagtct
    24481 tttgtgaaga ccaaattgac tttgaaaagc tcaaactcgt cctcggcgca gaaaaccttg
    24541 ccggtgcggg cgaacgttat caactcgatt gggcgggcaa aaccgcctcc tatctcaacc
    24601 tgcaaagccc cacttcccgc accctcgtcc cctgcaaaga agaatccgca gatttcgacg
    24661 gcacgcaaaa cgtgtttatc gaagccgaaa acctcgaagc actgaaaatc ctgcaaaaat
    24721 cctacgccgg cagcgtaaag atgatttaca tcgacccgcc ctacaatacc ggcaacgatt
    24781 ccttcgtcta tcccgacaaa ttctccgaaa gccgcgaaga atacgcccgc cgcgtgggcg
    24841 ataccgacga cgcgggttac ctgaagcgcg acggcgtgtt ccagggcgcg tggcgcaaaa
    24901 acggcaaaga cagtggccat taccacagca actggctctc tatgatgctg ccgcgcctgc
    24961 atctggcgaa aaccctgctg cgcgaagacg gcgtgatttt tatcagcatt gacgacaacg
    25021 aacaggcgca gttgaaattg ttatgtgatg aagtgtttgg ggcggagaat tttgtcggtc
    25081 agtttccttg gcgcaaacgc acggcaaaat cggacgtgcc gttcggcgtt tccgccgatt
    25141 atgaatatct gcttgcctat gcccgcagta atgcctttat tgccagtatc gatgcggaag
    25201 aacgcagata ttttgaaacc gatgatttcc cgaatcgccc gtggcggacg catgatttga
    25261 ctactcaaag aaatgccagt gaacgaccaa atagtttttt cactatggtt aatcctaaaa
    25321 caggcaaaga atatccggcg aatccaaatg caacttggcg tgttactaag gatacatttc
    25381 aagaatatta caatgcggga aaaatcgttt tccccgatga ttacgatttc ctcaaaatta
    25441 aaaaccccgt tatgcgttat tttaaagatg acgacatgaa gaaagacgga gagcgcttcg
    25501 gacgtaaggc agtcagtaca cggctacctg aaaatgtagg tatgtcgctg aacggtacaa
    25561 aagaaattac cgaactgttt aaaagcaagt tgtttgattt tcccaaaccg tcatccctaa
    25621 ttaaatattt actgcaaatc ctccatgttt cagacggcct catcctagac ttcttctccg
    25681 gcagcggcac caccgcccat gccgtgatgc aactgaatgc cgaagacggc ggcaaccgcc
    25741 gtttcatctg tgtccagctc cccgaagaaa ccgacgaaaa atctgaagcg cgcaaggcgg
    25801 gtttcgacac gattgccgaa attgccaaag aacgcatccg ccgcgcaggc aggcagattt
    25861 cagacggcct tcaagacgga tcggaaacag atacgggctt taaagttttc aaacttgccg
    25921 aaagcggttt caaacaatgg cggcagcccg agcagtccga tacggaagcc ctgcaacgcg
    25981 aattgtcgtt gaatatcgat tctgtgctgt ccgaaacgtc gtctgaaaat ctgctctacg
    26041 aattgatgct gcgtatgggc ttgaagctga cttgcaaggt ttcgttttta gacgacgtat
    26101 atttcgtgga agacgaggat acgggcgggc tgtacgcttt cctgctgaaa cgcgtggatc
    26161 aaggtttgat tgatgccgtg ctggcgaagc atcccgctaa ggtggcggca ttggacaggc
    26221 tgtttgaggg cgacgatgcg cttaaaagca ataccgtatt gcagatgaag gatgcgggcg
    26281 tgatgttcga gtgtgtgtag tcagccgaaa gcgggaagtg ccgtctgccg tcattcccgc
    26341 gcaggcggga atctagaaac tcaaagttgc aagaatttat cggaaatgac tgaaacccaa
    26401 cgaacctaga ttcccacttt cgtgggaatg acgatatatc agtttctgcg ccgacaggtc
    26461 tggattcccg cctgcgcggg aatgacggca aagaggtaga ttttccgcgc gacagcggat
    26521 agaaggaaat aaaaatggaa ctgaaatttg aagaactgcc ctatcagctt gatgcggtaa
    26581 atgccgttgc gaatcttttt gcagggcagc cgaaccatgc ccggacattc gatttgacca
    26641 gtcaggggac ggggcggttt gtcggcaacg gcttggattt ggattgggaa acgctaggcc
    26701 tgaatctgaa cacggtgcaa aagcaaaatg gacagcctga aacggaaatc ggcgcgcacg
    26761 ggctgaattt ttctctagaa atggaaacgg gtacgggcaa aacctatgtc tatctgcgca
    26821 cgatttacga attaaaccgc agttacggct ggaagaaatt tgttattgtc gtaccgagcg
    26881 taccgatacg cgaaggcgtg ttgaaaagcc tggaaattac gaaggcgcat tttgacggag
    26941 tgttcggcag gcccgtgatg cgttatgcgg aatattcaag cagccggcta tcggacttgc
    27001 gcacttttgc ggtaaacgac cacatcgaga ttttggtgat caatatccag tcgtttgaaa
    27061 aagatggaaa cgttatcaat aaggtaaacg aaagcgggga aacgccgatt aagtttattc
    27121 aggaaacgtg tcctatcgtt attatcgacg agccgcagaa tatggaaacg gaaggccgtc
    27181 tgaacgcttt ggaatcgctc aatccgctct ttaccctgcg ctattcggca acgcataaac
    27241 aaccgtacca caaggtttac agcctgaatc ccgtgcaggc atacaattta aagctggtaa
    27301 agcaaatcga agttttgccg gtattggcgg aaaacgatgt gaacggcgca tttgtggagc
    27361 tgctggaagt aaagcaggcg gccaaaggcc gtccgaaagc cgatttgaac attcattatc
    27421 aggataaaaa ggaaaccaag aagaaaaagg tatccgtcaa atgggaagac gatttgtttg
    27481 aaaaatcggg cggtaacgaa gcctaccgcc acggtttcat tatcgacggc atggatttcg
    27541 ataagcagga attggcgttc tcaagcggta aaaccattac gcgcggcgga gacggcgatg
    27601 cggtgcgcga tgaaattatg aaaaaccaaa tccgcgaaac ggtaagggag catttggcga
    27661 aggagaaacg gctgaatccg ctggggatta aggtgttgtc tctgttcttc atcgaccgtg
    27721 tggagaatta ccgtacagaa gacggaacgg caggcaagtt tgccctttgg tttgaagaaa
    27781 tctatcggga acttgcaggc aataccgata cggcaggcgt acacgacggc tatttctcgc
    27841 aagacaggaa aggccgtctg aaaaacaccg acggcacgac gcaggcggac aacgatacct
    27901 accgcctcat tatgcgcgac aaggaaaccc tgctttcctt tgacagcccg ctgcgcttta
    27961 tcttttccca ttccgcactg aaagaaggct gggacaaccc caacgttttc caaatctgca
    28021 cgctcaacga aacccgttcg ccgctgaaaa agcggcagga aatcgggcgc gggctgcgta
    28081 tcgccgtcaa tcaggaagga cggcgcgtgc gcgatgaaaa cgtgaacata ctgaccgttg
    28141 tgccgaacga aagctacgaa ttgtttgccg ccaatctgca aaaggaatac gaagacgaat
    28201 gcggtattaa atttgccgcc gaaaacatca aaaacggcaa taagaagaaa aaacagttat
    28261 tccgcaaaga tttcccgctc gaccccgaat ttcaggcaat ttgggacaag ttgaaataca
    28321 aaacgcgcta tcgcgtgcgc ttcgatacgg aagaattgat caaacaggct tcggaagcgg
    28381 tggcaaacct gccggaaatc caaaaaccga aaatccgcat ccgcaaggcg caattaaaac
    28441 aatcacaaac ctacggtatc gaaacagttg aaattgccgg ccgcagccgc gaaatggacg
    28501 ttcaatggga gattccggac atactcggcg agattcaaag aaaaacgggg ctgacgcggc
    28561 gcacggtgta cgaaatcatc atacagtcag gccgtctgaa agatgtagcc ggtaatccgc
    28621 aacgcttcat cgaccttacc gccgaagcgg taaaccgcag cctgcacgcg ttgatggcgg
    28681 caggaatcga gtatgagaga accggcgaag tgtacgacca aaagctgttg gaagatatgg
    28741 cgcgcgaagg tatcgagttt tacgaaaacg gttttacatt cacagtaagc aatccgcaaa
    28801 aaaccatttg cgaaaactac atccctttgg attccggcac ggaaaaagcc tttgcccaaa
    28861 attgcgagga ttatgatgcg aaaaatgaag gaggcgtggc gttttacttc aaactgcccg
    28921 gctggttcaa aatcccgacc ccgctgggca attacaatcc cgattgggcg gtggtaaaac
    28981 aggttcggga taaagtgtat ttcgttgcgg aaaccaaaaa caccggcaaa ggcattcaga
    29041 ccggcgtgga tatggacaag ctgcgccaaa atgagaggct gaaaatcaaa tgcggcaagc
    29101 ggcattttga agcgctgggc ggtttggcat accgtgttgt ggaaaaagtg ggggatttaa
    29161 agtcggaaaa agaggagcag gcttaaaaca aacaggtgta atcgtagtat ggcgatgttg
    29221 gattggggcg gcggcaaccg aagatgcgga tatggtaatg gctttgattg ggtaaaccga
    29281 tcggggactg aagattgttt cagacggtct gttgattttc ttatttcgga aatgggaaga
    29341 tggaaggcta ttgccctgtc agacgcgtcc aaagcctttg ccaccggctt cttttttcta
    29401 cattttccag tgcgacgatt tctttttcgg caagggtgta tccgttttgt ctgattttga
    29461 tttttcctaa aatctgcccc ttttttaccg gggcgggaat cggctgtatg gtttccaaaa
    29521 tttgttcggt tgttttcgct tccttatgtg gcagagtgat gtaggcttct ttgaggaagc
    29581 ctgcgcggac ggtttttttg ctgcctccgg aaatttggat ttgggcaacg gttttgcctt
    29641 tcggatatat tttgggcgta tcgaaggctt gcaatgcccg gttcagcagc ttgctgttgt
    29701 ctgatgcgcg tgtttccgcc gattccgaac ccaatgtgat gacaaggatg tgcctgccgt
    29761 tgccggagta tgacacggca aggttgtagc cgccgctttc tgtgtgtccg gctttcagac
    29821 cgtttacatt gttgtcccta tataaaagga tattgcggtt gttttgctct atatttttga
    29881 atttgaaaga tttgatggaa aacagcgggt aatattccgg aaagtcgcgc atcaatgctt
    29941 cagacagcag ggcgaggtct ttggcggtgg aaacctgtcc ttctctactc aagcctgtcg
    30001 ggtttttgaa tacagtgttc ttcatgccca agcgtcgggc ttctttgttc atttgttgca
    30061 caaaattttc aatcgagccg ttgcccagtc ggccgacaag ggttagggcg gcatcgtttg
    30121 cggatagtgc aatcatgcct tttaagagtt tgtcggtgct gaccgtatcg ccgggacgta
    30181 caaacattct gcttccttct gaagcccatg cggattcggg tatttttaag ttttcttcag
    30241 atcggatatt tcccgatttc atgtttttga aaaccagata tgcggtcatc agttgggtta
    30301 gtgccgccgg ttcgacaggg gtattgatgt ttttggcgga taaaatctgt ttgctttgaa
    30361 ggtcgataac gatgtgtgcc gctgtgaggg tttcgggtgt ttggaacgtg ggggcggcgt
    30421 gtaccgccgg tttgttgggc gcgggcgatg cggccgttgc gtgagaaacg cctaagatga
    30481 tggaaagcag gacgggcagg attttatgtg ctgtcatgaa atattctaat tgtgtgcgtg
    30541 tttcagtctg ccgattatac gcttagggtg tctgatcggg cggatttttc ttgctttcgc
    30601 gccgtcttgg gcgtatggtt ttgggttttg cgattttaat aaaccgatta tcccatattg
    30661 aattatgaac acgccccttc cttattccaa ttacctcatc cgcatcctga cggcatctgt
    30721 ctatgatgtc gcggtcgaaa cgcctttgga accggcacgc agcctttctg tacgtttgaa
    30781 aaacaacatc cttttgaaac gcgaagattt gcagccggtt ttttcgttca aaatacgcgg
    30841 cgcgtacaac aaaatgtcca agttgccgaa agatgcgctc gcttgcggcg tgattgcggc
    30901 aagcgcgggc aatcacgcac aaggcgtggc attgtccgca cagcgtttgg gttgccgtgc
    30961 cgttatcgtc atgccggaga ctacgccgaa aatcaaagtg gatgcggtca aaagccgtgg
    31021 cggcgaggtg gttttgcggg gcgtttcata caacgatgcc tacgattatg cgatggagtt
    31081 ggcggaaaaa gaagggttaa cctatatcgc gccgtttgat gatcctgatg tgattgcggg
    31141 acaggggacg gtggggatgg aaattgtcag ccagcatccc gatccaatcc gcgccgtatt
    31201 cgtaccgata ggcggtggcg gtttggcggc gggcgtggcg gcatttatca agcaggtccg
    31261 ccccgaaatc aaagttatcg gcgttcagac caacgattcc tgctgtatga agcagtcggt
    31321 cgaagcgggt gaaatcgtcc atttgaaaga tgtcgggctg ttttcagacg gcactgcggt
    31381 caaagtcgtc ggaaacgaaa ccttccgcct ctgcaaagaa cttttggatg aaatcattac
    31441 agtcgatacc gatgcggttt gcggcgcggt caaggatatt ttcgatgaca cgcgcagcat
    31501 taccgagccg gcgggcgcgt tggcgttggc gggtctgaaa gcctatatcg cccgagaagg
    31561 cgcggaaaac caaaccctga ttgccgttac cagcggtgcg aatatgaatt ttcaccgttt
    31621 gcgccacgtt tcggaacgga gcgaattggg cgagggcaac gaaggtattt ttgccgttac
    31681 catccctgaa gaacgcggca gcttccttaa gtttgtcaat atattgggaa ataggaatat
    31741 taccgagttc aactaccgct acggggacga tgaaaaagcg catatctttg tcggacttca
    31801 agcggcaggc ccgcaggatt tggcggttat cggcagccgg ttggatgagg cgggattgcc
    31861 caatgtcgac ctgaccgatg atgagattgc caaaatccat atccgctata tggtcggagg
    31921 gcggacggac aaagtagaaa acgagcgtct ggtcagtttt gagtttccgg agcgtccggg
    31981 ggcgttggcg cgttttttga accatatgca gggcggttgg aacattacgc tcttccatta
    32041 ccgcaaccac ggtgcggatt acgggcggat tttggtcggt atcgatgtgc cgccgcacga
    32101 tgccgccgca tttgacggtt tcttggaaag tctgggatac agctatcacg aggaaacgca
    32161 aaatgccgcg tacaagctgt ttcttgcctg acgcttgaaa gcacaatgcc gtctgaaagc
    32221 ctttcagacg gcattgcgct ttcatggtta aatcgaatat tcaatcagtt cgttttgaga
    32281 gaagacataa acctgtttcg gaatcagctt caattctttg ccttcggcga ttgggtaacg
    32341 cgcggcatcg ctgccagcca gcgtgatatg cacgtcttgt ttgccgtgtt tcaccaaaat
    32401 atgcgtcaat gccccgacgg cgtggacttt ttcgatttcg gcacaaatca tcggtgtttc
    32461 gtgttcggcg gcgatctgcc attcgtgcgg gcggatatag ccggtggcgg tttgttcctg
    32521 ccatttgtat tgtgcgtcca atttccacgc gaagccgtta taatgccaga agcctttttc
    32581 gatgcgtcct tcaaaagcgt cggtttcgcc gaggaactcg gtaacgaagg cattttcggg
    32641 tttgcggtaa atagcttcgg cgctgccggt ttgttcgatt ttgccgtggt tcatcacgac
    32701 gatttcgtcg gaaacttcga gggcttcttc ttggtcgtgc gtaaccagaa tgctggttac
    32761 acccaggttg tgatggatgt cgcgcagcca ggtgcgtaat tctttgcgta ctttggcatc
    32821 caacgcgccg aagggttcgt ccaaaagcag gagtttcggt tcgaccgcaa gcgcacgggc
    32881 gagggcgatg cgctggcgtt gcccgccgga gagttggtgc ggataggatt ttgccaaatg
    32941 cgagagctgc acgagcttga gcaattcttc gactttggcg cggatttgtc ctttggacgg
    33001 gcgttcggac ttgggcaata cggtcaaacc gaaggcaacg ttgtcaaaca cgttcatatg
    33061 gcggaaaagg gcgtagtgtt ggaacacgaa gccgactttg cgctcgcgca cgtgcttggc
    33121 ggttacgtct tgcccatcaa acagaatctt gccgccgtcg gcgttttcca gtccggcgat
    33181 aatgcgtaaa agcgtggttt tgccgcagcc ggacgggccg agcagggaaa cgagtttgcc
    33241 ggtggggacg ttgaggttga tgtttttcag cgcgtgaaaa ttgccgaagt gtttgtttaa
    33301 gttttggatg gtgatactca tactgcgttc ctttcggcgg cggcgagttt tttgtcttgt
    33361 aatttggtga tgatgttctg caccgccagc gtcgccagtg ccaaaagtgc caatacgccg
    33421 gagagggcga atgcgccggt gaagttgtat tcgttgtaga agatttcgac caaaagcggg
    33481 acggtgttgg tttcgccgcg tatgtgtccc gataccacgc tgaccgcgcc gaactcgccc
    33541 atcgcgcggg cgttggtgag gatgatgccg tagagtaacg cccatttgat gttgggcagg
    33601 gtaacgcgcc aaaacatctg ccagccgctt gcgccgagta tcaatgccgc ctgttcttcg
    33661 ctgtcgccct gtgcctgcat cagcgggatg atttcgcgtg cgacaaaggg gaaggtaacg
    33721 aacagcgtcg ccaaaacaat accggggatg gcgaagataa tctgtatgcc ttgcgcttcg
    33781 agccggccgc ccaatgccgt gtgcgcgccg aacaataaga cgaacattaa accggccacc
    33841 acgggcgata cggaaaacgg caaatcgagc agggtagtca gcaactgctt gccgcgaaaa
    33901 tcaaaacggg tcagcagcca cgccatcgcc acgcccaaga cggcattgac ggggacgaca
    33961 atcagcgcgg taatcagcgt caatttgatg gcagaccacg cttcgggatc gtttaaggat
    34021 ttcaggtaca aatcccaacc gccttttaag gcttcgtaaa acacggcgac gagcggcacg
    34081 accagcatca gcagcagaaa gcccagcgcg gcggcaatca gcaacacgcg cagccggcgc
    34141 ggttcggtca ggttgggatt ggcggaatag ggtttcatgg cggtggtgtt ctctggttgg
    34201 ttttaaaatg tttggaggtc gtctgaaaaa ctgtgttttt gggagcggat tcgttttcag
    34261 acaacctttt attgattaag ggaataaaaa cgtctagcta ccgtcattcc cgcgcaggcg
    34321 ggaatccacc acagggcaac aggaaaacag aaaataaata aggcagccga aattcaccaa
    34381 tggattcccg cctgcgcggg aatgacggta acaggtattt cagacgacct caacccttcg
    34441 cgcccgaacg cctgcccaac gcccacagca tcacgttcag cgcaaacaga atgacaaacg
    34501 aaaccagcag cataaacaac gccaccgccg acgcgccctg tacgtcgaac tgttccagct
    34561 tgcccgtaat aatcagcggc aggatttcag aaaccatcgg aatattgccc gcgataaaaa
    34621 tcaccgagcc gtattccccc gttgcccgcg cgaacatcat tcccgcgccg gtcaagagtg
    34681 ccggcgtgat ttccggcagc agcacgcggc ggaacgtcgt aaagcggttt gcgcccaaag
    34741 tcgccgccgc ttcctcatat tcgcccgata attcctccaa caccggctgc acggcgcgga
    34801 cgataaaggg caggctgacg acgaccagcg caatccaaat gccgacgggc gtaaacgcga
    34861 ttttgatgcc caaaggctcg aaaaaacggc ctatccaacc gttgggcgca tacagggttg
    34921 ccaacgcgat acccgtaacc gccgtcggca gcgcaaacgg caaatcaacc agcgcgttcg
    34981 ccagaccctt gcccggaaat tcatagcgca ccaataccca cgccaccagc gtgccgaaca
    35041 cgacattggt cagcatcgca taaaacgaca tccgcaaact cagccacacc gccgccaaca
    35101 cgttcggctc ggcaatcgtg ttccaaaagc cgccccagcc gatttccgcc gccttcgccg
    35161 ccatcatcgc aaacggcaag accacaagca gcgacaggca caatacggtc agcccgaggc
    35221 tgagtttgaa gccgggcagt acgccgggcg ttttgagcgg taacataaaa caatgctgaa
    35281 aataaggaaa aggaaggact actttaacga tgccgtctga aaaacggaaa gaatggaaag
    35341 tttggtacaa agacgaattt gttataaagc ggttggcagt ttctcaagcg ggcgcgatgt
    35401 tttaaaatat agtggattaa atttaaatca ggataaggcg acgaagccgc agacagtaca
    35461 aatagtacgg caaggcgagg caacgccgta ctggtttaaa tttaatccac tataacgcct
    35521 tgttttgacg gaaaaccatc atataaagga acacttatgc agattttatc ttttcagccg
    35581 gacattgcgg aacgtatgct ggaaggtacg gaaggcgagt cggtcaacga aaacgcacaa
    35641 ttcgtccgta cggacaacgg ctattggatt gcgtggcatg aaggcgtagc ggcactgctt
    35701 gcgcccgata cgccgccggg cattccctgt ttttgggtgg aaggcgcgga aagccttgaa
    35761 gagttgtgcg tcatggtgga acgcggcgag tttgacgaag tggaagagtt tgacggcgat
    35821 gacgacgaat ggctcgaaac ggcacagggt tgcggacacc acggcgaagc ttgcgcctgc
    35881 ggacattaaa ggcattgcag gcttgccgca agggggcaag gctttgccgt tttttaaata
    35941 atcggtggat tgcatctgtt tgcaatgggc tgaacgcttc cctttgtatt ttattgaata
    36001 aaaaaactta tcttggtatt ataattaagg cagcatcaat tattttggga tggcaataaa
    36061 cgcaaagcat tgatttgcgc cgattgcaga cttattatag caggttgcgg cgcggactta
    36121 atgggaattg tttatagagc atgatggtct gaaacatcat taattaaaaa taccattaat
    36181 ccgatttata tttatttcaa tttcaatgga aaaacatcaa tgacaatgat tttaagcatt
    36241 ttaagcctgt tttttatcat cagactgtta tttttagccg tctctattaa acatgaaaaa
    36301 gccttgattg ccaaaggggc gaaacaatac ggaaaaacca attccacgct gcttgcggca
    36361 gttcatacgc tttattattt ggcgtgtttt gtttgggtat ggctttctga cactgctttt
    36421 aatggcatat ccttgattgg tacgctgacg gtgatggctt cgtttgtgat attgtcattg
    36481 attattaagc agttggggga gatttggacg gttaaaatct atattttacc aaatcatcaa
    36541 attaatcgtt cgtggttgtt taaaacattc cgccacccca attatttttt aaacatcata
    36601 cccgaactga ttggcatcgc cttattatgt caagcgtggt atgttttatt gattggcctg
    36661 cccatttatt tgctggtctt atttaagcgt atccgacaag aagaacaggc gatggcaaca
    36721 cttttttaac ccgtttcatc aattatag
//
LOCUS       DAXHMX010000003        34782 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHMX010000003 DAXHMX010000000
VERSION     DAXHMX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.003"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            171..665
                     /locus_tag="ACFM4S_000101"
     CDS             171..665
                     /locus_tag="ACFM4S_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049867.1"
                     /translation="MENRFAKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEIFKMSVLEDWERSGKPLDWEKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            785..1918
                     /locus_tag="ACFM4S_000102"
     CDS             785..1918
                     /locus_tag="ACFM4S_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049868.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESIRTEENLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYHIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(2005..2280)
                     /locus_tag="ACFM4S_000103"
     CDS             complement(2005..2280)
                     /locus_tag="ACFM4S_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049869.1"
                     /translation="MNEGEVVLTPEQIQTLYGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(2297..3070)
                     /locus_tag="ACFM4S_000104"
     CDS             complement(2297..3070)
                     /locus_tag="ACFM4S_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6049870.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNNRINQTEEDNYSTGVGSCINGFP
                     VRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(3469..3744)
                     /locus_tag="ACFM4S_000105"
     CDS             complement(3469..3744)
                     /locus_tag="ACFM4S_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6049871.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(3741..5024)
                     /locus_tag="ACFM4S_000106"
     CDS             complement(3741..5024)
                     /locus_tag="ACFM4S_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6049872.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRVPILGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(5175..6593)
                     /gene="glnA"
                     /locus_tag="ACFM4S_000107"
     CDS             complement(5175..6593)
                     /gene="glnA"
                     /locus_tag="ACFM4S_000107"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6049873.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWSSGLHLDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIKVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            6897..7706
                     /gene="aroE"
                     /locus_tag="ACFM4S_000108"
     CDS             6897..7706
                     /gene="aroE"
                     /locus_tag="ACFM4S_000108"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6049874.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHRQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLILLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVNPEIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKQTADGLGMLVGQAAASYALWRGFTPNIRPVIEYMKAM"
     gene            7708..8409
                     /gene="mtgA"
                     /locus_tag="ACFM4S_000109"
     CDS             7708..8409
                     /gene="mtgA"
                     /locus_tag="ACFM4S_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6049875.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGIFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(8734..8809)
                     /locus_tag="ACFM4S_000110"
     tRNA            complement(8734..8809)
                     /locus_tag="ACFM4S_000110"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8774..8776),aa:Phe,seq:gaa)
     gene            complement(9000..9734)
                     /gene="lptB"
                     /locus_tag="ACFM4S_000111"
     CDS             complement(9000..9734)
                     /gene="lptB"
                     /locus_tag="ACFM4S_000111"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6049876.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(9808..10338)
                     /gene="lptA"
                     /locus_tag="ACFM4S_000112"
     CDS             complement(9808..10338)
                     /gene="lptA"
                     /locus_tag="ACFM4S_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6049877.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(10319..10900)
                     /gene="lptC"
                     /locus_tag="ACFM4S_000113"
     CDS             complement(10319..10900)
                     /gene="lptC"
                     /locus_tag="ACFM4S_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6049878.1"
                     /translation="MKIRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(10897..11433)
                     /locus_tag="ACFM4S_000114"
     CDS             complement(10897..11433)
                     /locus_tag="ACFM4S_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6049879.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(11498..12472)
                     /locus_tag="ACFM4S_000115"
     CDS             complement(11498..12472)
                     /locus_tag="ACFM4S_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6049880.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTIAAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            12568..13623
                     /gene="tal"
                     /locus_tag="ACFM4S_000116"
     CDS             12568..13623
                     /gene="tal"
                     /locus_tag="ACFM4S_000116"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6049881.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALEILVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWEQYFTTPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDNL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLANRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            13777..14067
                     /locus_tag="ACFM4S_000117"
     CDS             13777..14067
                     /locus_tag="ACFM4S_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049882.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYRELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            14084..14971
                     /gene="gluQRS"
                     /locus_tag="ACFM4S_000118"
     CDS             14084..14971
                     /gene="gluQRS"
                     /locus_tag="ACFM4S_000118"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6049883.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQTGARRGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(15041..16051)
                     /gene="dusA"
                     /locus_tag="ACFM4S_000119"
     CDS             complement(15041..16051)
                     /gene="dusA"
                     /locus_tag="ACFM4S_000119"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6049884.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(16082..16573)
                     /gene="dtd"
                     /locus_tag="ACFM4S_000120"
     CDS             complement(16082..16573)
                     /gene="dtd"
                     /locus_tag="ACFM4S_000120"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC6049885.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(16647..17405)
                     /locus_tag="ACFM4S_000121"
     CDS             complement(16647..17405)
                     /locus_tag="ACFM4S_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6049886.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(17476..18342)
                     /locus_tag="ACFM4S_000122"
     CDS             complement(17476..18342)
                     /locus_tag="ACFM4S_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6049887.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEIKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(18391..18669)
                     /locus_tag="ACFM4S_000123"
     CDS             complement(18391..18669)
                     /locus_tag="ACFM4S_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6049888.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(18669..18959)
                     /locus_tag="ACFM4S_000124"
     CDS             complement(18669..18959)
                     /locus_tag="ACFM4S_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6049889.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(18962..19492)
                     /locus_tag="ACFM4S_000125"
     CDS             complement(18962..19492)
                     /locus_tag="ACFM4S_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6049890.1"
                     /translation="MKFVSDLFSVILFFATYTVTKNMITATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            19781..22192
                     /gene="tspA"
                     /locus_tag="ACFM4S_000126"
     CDS             19781..22192
                     /gene="tspA"
                     /locus_tag="ACFM4S_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6049891.1"
                     /translation="MKNNRQIKLIAASVALAASFQAHAGLGGLNIRSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSAQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAHNGKTHTVRKGETLKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAADDLSALLQPAEALSVEENITETVAETPDFNATADDLS
                     ALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENVTETVAETPDFNATADDLSA
                     LLQPAEVPAVEENAAEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(22365..22781)
                     /gene="gloA"
                     /locus_tag="ACFM4S_000127"
     CDS             complement(22365..22781)
                     /gene="gloA"
                     /locus_tag="ACFM4S_000127"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6049892.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(22806..23975)
                     /gene="lapB"
                     /locus_tag="ACFM4S_000128"
     CDS             complement(22806..23975)
                     /gene="lapB"
                     /locus_tag="ACFM4S_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6049893.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(23986..24300)
                     /locus_tag="ACFM4S_000129"
     CDS             complement(23986..24300)
                     /locus_tag="ACFM4S_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6049894.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            24610..25608
                     /gene="ilvE"
                     /locus_tag="ACFM4S_000130"
     CDS             24610..25608
                     /gene="ilvE"
                     /locus_tag="ACFM4S_000130"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6049895.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLSYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(25852..26637)
                     /gene="fabI"
                     /locus_tag="ACFM4S_000131"
     CDS             complement(25852..26637)
                     /gene="fabI"
                     /locus_tag="ACFM4S_000131"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6049896.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(26792..27613)
                     /gene="dapD"
                     /locus_tag="ACFM4S_000132"
     CDS             complement(26792..27613)
                     /gene="dapD"
                     /locus_tag="ACFM4S_000132"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6049897.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(27824..29467)
                     /gene="pgi"
                     /locus_tag="ACFM4S_000133"
     CDS             complement(27824..29467)
                     /gene="pgi"
                     /locus_tag="ACFM4S_000133"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6049898.1"
                     /translation="MNAFTRAWRELEQHCQDTRHILLRDRFACEPDRFDRMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAAGLGRKMHDLWTGAKVNNSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALKFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETTRHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGVIWNINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAV"
     gene            29977..31209
                     /gene="pilG"
                     /locus_tag="ACFM4S_000134"
     CDS             29977..31209
                     /gene="pilG"
                     /locus_tag="ACFM4S_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6049899.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            31283..32143
                     /locus_tag="ACFM4S_000135"
     CDS             31283..32143
                     /locus_tag="ACFM4S_000135"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6049900.1"
                     /translation="MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            32145..32777
                     /gene="coaE"
                     /locus_tag="ACFM4S_000136"
     CDS             32145..32777
                     /gene="coaE"
                     /locus_tag="ACFM4S_000136"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6049901.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFIILIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            32770..32979
                     /locus_tag="ACFM4S_000137"
     CDS             32770..32979
                     /locus_tag="ACFM4S_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6049902.1"
                     /translation="MTESRQTRLQVKCPTCQTTVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSDQTESLLEISEPDGAYR"
     gene            complement(33064..34740)
                     /gene="pilB"
                     /locus_tag="ACFM4S_000138"
     CDS             complement(33064..34740)
                     /gene="pilB"
                     /locus_tag="ACFM4S_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6049903.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHPL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         8307 a         9510 c         8868 g         8097 t
ORIGIN      
        1 aataacaaat tatcaaacaa agctctgatt aaaaacccaa caatcaaaat gccgtctgaa
       61 gcggtattcg gctttcagac ggcattttct ccgattagca caggatgccg gggcttgccg
      121 gcttgatttt gccgaaattg ttggcacgca attgggaggg gcggcaacct atggaaaata
      181 gatttgcaaa aatcgttaag gatattttta gttttaccca atttgtttcc gaagcggaaa
      241 ataaaattcc taaccaatcc tctcgttatt acgattgctg gtgggaattg gaaatcttga
      301 atgcttccgc attggaagat tgggaaatat cgggaaagcc tgaaaaatgg gagaaatgga
      361 cagaaaaata tcagaccgaa gcgttacagt cagtcaagca tctatttgcc gaagaagttc
      421 ctgaaaataa tatagtttgc gatatttctc attttttgca ttttgccgat tgggcagaag
      481 ccgacattcc caatcaatca cgtttatatg ccgaatattg gtgggaaatg gaaattttca
      541 aaatgtcggt tttagaagat tgggaacgct cgggaaaacc gttagattgg gaaaaatggc
      601 aaaggcaata tcgatctgaa gcatttgaat tagttaaaaa attgaaccga agcaaacaag
      661 agtaacaagc cgcagcctgc ataggggttt ttaaagtacg gcagcaaccg tagattaaat
      721 taaaaatcaa acaatcaaag ctcgtctgat ataatcgata tattctgtta aacgaaaaat
      781 aaatatgttt aaaaaattca aaccggtatt gttgtcattt tttgcacttg tatttgcctt
      841 ttggctggga acgggtattg cctatgagat taatccgcgt tggtttttga gcgatacggc
      901 aactgaaata cctgaaaatc cgaatgcttt tgtggcgaaa cttgcccgcc tgttccgaaa
      961 tgccgacagg gcggttgtca tcgtgaagga atcgataagg acggaggaaa atcttgccga
     1021 aactgtggat gacggtccgt tgcagtcgga gaaggattat cttgcactcg ctatccggct
     1081 cagtcgtttg aaagaaaagg cgaaatggtt tcacgtaacg gagcaggaac atggggaaga
     1141 ggtttggctg gattaccata tcggcgaggg cggtttggtt gcggtttcgc ttttgcaacg
     1201 ctcgccggaa gcgtttgtta atgccgaata tctgtatcgg aacgatcgtc cgttttctgt
     1261 aaatgtgtac ggcggaacgg ctcacggaga aaattatgaa acgacaggag aatatcgggt
     1321 tgtttggcaa ccggacggtt cggtatttga tgcgtcgggg cgcgggaaaa tcggggaaga
     1381 tgtttatgag cattgtctcg ggtgttatca gatggcccag gtatatttgg cgaaatatcg
     1441 ggatgtcgcg aatgatgagc agaaggtttg ggacttccgc gaagagagca accggattgc
     1501 gtcggactcg cgcgattctg tgttttatca gaatatgcgg gaattgatgc cccgggggat
     1561 gaaggcgaac agtcttgtgg tcggctatga tgcggacggt ctgccgcaaa aagtctattg
     1621 gagtttcgac aatggaaaaa aacgccagag tttcgaatat tatttgaaaa acggaaatct
     1681 ttttattgca caatcttcga cggtagcatt gaaagcggat ggcgtaacgg cggatatgca
     1741 gacctatcat gcgcaacaga cgtggtattt ggatggcggg cggattatcc gcgaagagaa
     1801 acagggagac agactgcctg attttccttt gaacttggaa gatttggaaa aagaggtgag
     1861 ccgttatgca gaggctgcgg cgagacgttc gggcggcagg cgcgaccttt ctcactgaac
     1921 gcaaatgccg tctgaaaccg gttttcagac ggcattggag agtcaaacgc tttttgcaat
     1981 gccgtatatg ccgaatccga ttcgctacaa attgaaaacc acaatccaaa aaacggaaat
     2041 acacattaaa cggatctttt taccgaccgt atcatcccat aggttttcca cacctttttt
     2101 catccataaa ttcaaaccat ttaagtttgc atccttaccg acaattgcag cagcatcatc
     2161 cgcataacgg tcacccaaat tagccaaata acgccaaccg ccataggtat cgccacggga
     2221 agcataacca tacaaggttt ggatttgttc tggtgttaaa acaacttcac cctcattcat
     2281 aataagatcc tttaaattat tttttacttg aattgtttac aaaactctgt accaattgct
     2341 ctacttgttg gtgataacct accaactcct gctgtgtgag ctgctgcttt tcccgaataa
     2401 acgggtaata ccgtaccggg aaaccgttaa tacaggaacc tacgccagtg ctgtaattat
     2461 cttcttcagt ttggtttatt cgattattcc gatgagtaat aatcgccgga ttttctcctt
     2521 ttttaatata gtaaaattca tgaaaatatg gattttcagg aatatgtctt ttatcttcat
     2581 aagcctcgta ttcaggatag gcagatggca tttttttaac cccgtaagta agcaatcttt
     2641 ctcccatagt cgtgcttaaa ctacacgcat cttttcggat aacaatatcg tccacgctat
     2701 ccaaaccgtc gcgtagaaat tggataaagt gtgctttgtt ttgctggttg tatggctcaa
     2761 gaacccaacc ttggtcggtt tttttgtaat agacgctaat ctctatcaaa ttaatggagt
     2821 aagcatcctg cccgggcgtg tcggaaagcc attttgtggc attgtcttgc gtataccgtg
     2881 caagatagcc ataggggata accattttgg tgccctgaaa atcaaatgta accgtatgtt
     2941 caaatcctgt cattggctcg ggattgttga aactggtttg ttcattaaca ggttcacatg
     3001 aggtcatagt aaaaacactc ccaataaaca aaataaaaag ttgataaata gtagtagcat
     3061 atggtctcat aatttcaagc ctagaaatta gttaaagaat atattatggt agatactaac
     3121 tctttaagtt gacatattgt gcgatatcga gggctgtacc gtattttccc atcgtatttt
     3181 cccatcgtat tttcccgttc tttatatgag cggcaggcat cctcataaaa gaagggatac
     3241 cgataaggaa aaatacccca gcataaaaaa tatactgatt aatcaataca aatccaacaa
     3301 acagttcatc gtattctttt ctgttggcgt ataaaaaata ctgatatatg ccttcacttt
     3361 catagggcga cgctttatgt accgtcctgt gtgttgaaac gtcggttttc cttaccgcct
     3421 gccccgcttt tccctgattg ctgccaactg ccgtccgaac ttgaaccgtc aggtttcaga
     3481 cggcattttt tctgcctgct cccacaattg ccgtttttgt gcggcggtca gttttttgac
     3541 ggcgatttcc tgcctgagtg ccgttccttt atccatcccg cctgcaacga tacgcatcgc
     3601 caccggtttg aatacgcggg tatatttcgc gcctttaccg gttgtgtggg cggcaagccg
     3661 ctgttgcgga ttcgggctga tgccgcaata gaacgcgctg ttttcacaca atatcaggta
     3721 aacgctccag tttgacgcgt tcatctgcct gcatcctgag ttttctcccc gttccaaggc
     3781 gcaactttca gcagcagcag catacccggc agggcaagta tgaaacacag ctggaaaaac
     3841 ggtacatacc cgagccattc aatcagataa ccggcaaagg aattgatgac cgtacgggga
     3901 acagcggaca ggctggtaaa cagtgcaagc tgcgttgcgg taaatgcggg attggtctcg
     3961 cgcgccatat acgatacaaa cgccgccgtc cccaaaccca cgccgactgc ctctgcgccg
     4021 ataactgccg ccagcatcag cctctcgcct gtgccgaccg tgtcgaaatg tccgaaccct
     4081 gccagccata caaaccccaa aacggttaca gcctgcaccg cgccaaatat ccacaaggct
     4141 ttgtttacgc cgattttcag catccacacg ccgcccaaga tacctgccgc cactgccggc
     4201 cacagtcctg cgtttttggc aatcaaaccg atgtcggtct tgctgaaacc catatccaga
     4261 taaaacggcg ttgccaacgc ggttgccata ctgtcgccga gtttgtaaag gaagataaac
     4321 agcagcacgc atacagccga agcgatgccc ttgcgtgtaa aaaattcttt aaacggctct
     4381 accactgtct gcttcaacgt tttaggaacg gaaggcggca acacgggttc gcgcgcgaga
     4441 aacagcgtca tcagaaggcc gggcagcata aataatgaag tgataacaaa tacttctgac
     4501 cacggcatcc tgtctgccaa caccaaactc aatgaaccgg gaatcagggc ggcaatccgg
     4561 taggcgttca catggactga gttgcccaaa cccaattcct cgtccgacaa aatctcgcgc
     4621 ctgaacgcat ccaatacaat atcctgactg gcggaaaaaa aagcgacaag caccgacaag
     4681 ccggcaatca acggcagatg attccggggg tttaaaaagg cataggccgc caaagccgcc
     4741 agcaaccctg cctgcgtcaa cagcatccac ccgcgccgcc ggcccaaaat gggaaccctg
     4801 accgcgtcca tcagcggaga ccacaaaaat ttccaagtaa acggcagacc gatcagcgcc
     4861 atcagcccga tgctcttcaa atccacctgc tcgctgcgca accatgccgg aatcaggtta
     4921 atcagaaagt acagcggcag ccccgaggta aaccccgtaa aaatacagac aagcatattt
     4981 cgggaaaaga tttgcccgat aaaacctgat ttcgatacag tcatggttca aacagccaag
     5041 aaataaaagc ctattgtagc aaatatcgcc caaccgctga aaaacaccga ccctgctccg
     5101 ccgttttgcc gttcaaatgc aaaatgccgt ctgaaacttt gttcagacga catttctact
     5161 gaaacctgag agttttacag gctgtaatac atttcaaatt ccagcggatg cggtgccatg
     5221 cggatgcggc ggacatcttc ctctttaaag gcgatgtagc tgtcgatcca gtctttgctg
     5281 aatacgccgc cgcgcaggag gaattcgtgg tcggctttga gggcggcgag ggcttcttcc
     5341 aaagaggcgc agacggtcgg caccaatgca tcttcttccg gcggcagatc gtacaggttt
     5401 ttatcggcag ggtcgcccgg atgaattttg ttttgaatac cgtccaaacc ggccatcaac
     5461 aaagcggcga acgccaagta tgggttggcg gtcggatcgg ggaaacgcgc ttcgatgcgg
     5521 cgcgccttgc tgctgttcac ggacggaatg cggatggaag cggaacggtt tttggcggaa
     5581 taagccagtt tggtcggcgc ttcaaagtgc ggcacaaggc gtttgtagga gttggtggac
     5641 ggattggtaa tcgcgttcag ggctttggcg tgtttgatga tgccgccgat gtaataaagg
     5701 gcggtgtcgc tcaaaccggc atagccgtcg ccggcgaaca ggttttgacc gtctttccag
     5761 atggattggt gaacgtgcat accgctgccg ttgtcgccca taatcggttt gggcatgaag
     5821 gtggcggttt tgccgaagtt gtgggcaacg ttttgaatca catatttcat gtcttgggtt
     5881 tggtcggcgc gtttaaccaa agtggcgaag cgcgtgccga tttccatttg gctgccggta
     5941 ccgacttcgc tgtggtggac ttcgactttg atgccgagtt cttccaaaat gttcaccatg
     6001 gcggagcgca aatcttgacc gcagtcaatc ggggcaacgg gcgcgtaacc gcctttgacg
     6061 gtcggacggt ggccagtatt ttgaccgtcc aaatgcaagc cgcttgacca tgcgccgctt
     6121 tcggaagtga tttcgtaacg ggttttgtgc atgtcggttt caaattctac gccgtcaaaa
     6181 acgaagaatt caggctcggg gccgaaatag gcggtgtcgc cgatgccgga agatttcaaa
     6241 taggcttcgg cgcggcgggc gatggagcgc gggtcgcggt cgtaaccctg accgtcggca
     6301 gggtcgatga cgtcgcaggt aatgacgacg gtaacatcat cataaaaagg atcgacgaag
     6361 gctgtagacg catcgggacg cagctgcata tcggaagcct gaatgccttt ccagccgccg
     6421 atagacgaac cgtcaaacgc ctgaccgttt tcgaaccact cttcggggtc ttccaacacg
     6481 atgcgcgcag gcacggtaaa gtggtgctgc ttgcctttgg tatcggtaaa gcgcaaatcg
     6541 acaaagcggg cttcgctttc ttcaatcaat tttacggcgt ttttaataga catttttcag
     6601 ctcctggaaa agggaaatag caaaatatgc ggaacgcggc ggcttgacgc aaagtcggca
     6661 tgattctacc acgtccatgc cgtctgaaac tcgccctgcg tcaaatttca agctggaaaa
     6721 tatagtttca gacggcaaga ttgtcaacaa tatggataaa attgcatatc atgtccggat
     6781 ttctccccag cttatacagg gaaaaaccgt accgcctgac aaccgtttca gacggcatcg
     6841 tttccatctg tacgccgccg ttataaaata gccttcccgt cttttggaac acgaccatga
     6901 ccgccctccc ccgttactcc gttttcggca accccgttgc acacagtaaa tctccgcaga
     6961 ttcatcggca atttgccctt caggaaggcg ttgacatcga atacggacgc atttgcgccg
     7021 acatcggcgg tttcgctcag gcggtttcga cattttttga aacaggcggt tgcggggcaa
     7081 acgttaccgt accgttcaaa caggaagcgt ttcatctggc ggacgaacat tccgaccgcg
     7141 ctttggcagc aggtgcggtc aatacgctga ttctgttgaa aaacggcaag ctgcgcggcg
     7201 acaatactga cggtatcggt ttggccaacg acatcacgca ggttaaaaac attgccatcg
     7261 agggcaaaac tattttactt ttgggcgcgg gcggtgcggt gcgcggcgtg attcctgttt
     7321 tgaaagaaca ccgtcctgcc cgtatcgtca ttgccaaccg tacccatgcc aaagccgagg
     7381 aattggcgca gcttttcggc attgaagccg tcccgatggc ggatgtgaac ggcggttttg
     7441 atatcatcat caacggcaca tccggcggtt tgagcggtca gcttccggcc gtcaatcctg
     7501 aaattttccg cgactgccgc cttgcctacg atatggtgta cggcgaagcg gcaaaaccgt
     7561 ttttggattt tgcccggcaa tcgggcgcga aacaaaccgc cgacggactg ggtatgctgg
     7621 tcggtcaggc ggcggcttcc tacgccctct ggcgcggatt tacgcccaat atccgccctg
     7681 ttatcgaata catgaaagcc atgtaatatg ttccgcatcg tcaaatggct gattgccctg
     7741 cccgtcggca tctttatctt tttcaatgcc tatgtgtacg gcaacatcat cacctaccgc
     7801 gccgtcgcgc cgcatcggac tgcctttatg tcgatgcgga tgaagcagtt tgaacaggaa
     7861 ggtcgcgatg tcgcactgga ttaccgctgg gtaccctaca accgcatctc ggtcaacctg
     7921 aaaaaagccc tgattgcttc cgaagatgcc cgtttcgccg gacacggcgg cttcgattgg
     7981 ggcggcattc aaaacgccat caggcgcaac cggaacagcg gcaaagtgaa ggcgggcggt
     8041 tcgaccatca gccagcagct tgccaaaaac ctgtttttaa acgaaagccg cagctatatc
     8101 cgcaaaggcg aagaagcggc gattaccgcg atgatggaag ctgttaccga caaagacagg
     8161 atttttgaac tgtatttaaa ctcaatcgag tggcactacg gtattttcgg cgcggaagcc
     8221 gcgtcccggt atttttatca aatacccgcc gccaaactga gcaaacagca ggcggcgaaa
     8281 ctgacggcgc gcgtccccgc cccgctctac tacgccgacc atccgaaaag caaacggctg
     8341 cgcaacaaaa ccaatatcgt gctcagacgc atgggttcgg cagaattgcc tgaaagcgac
     8401 acggactgat tgtttcagat atggaaatgc cgtctgaact gcggttcgga cggcatatgt
     8461 tttctggttt ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
     8521 acaaatagca cggaatcgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
     8581 agctaaggcg agccaacgct gtaccggttt aaatttaatc cactatacct gcccgacagc
     8641 cgcaaagacc gtatcataca tagcaggcat tgcggtgcgg gattactgcc gcaaacggca
     8701 ggcaaaaaaa ataaccgctt caaggcggtt ttttggtggc cagaggcgga atcgaaccgc
     8761 cgacacacgg attttcaatc cgttgctcta ccaactgagc tatctagcct ttatctgtct
     8821 tgttaagaga tgcgtattaa accaaatttc gggacaacac gcaagcggat ttgtcgatat
     8881 tttttatcca accgactaag tcgatgtttt aagtttaaat taatttcaca gaaaaatagt
     8941 gcaaatcggt tttttgccgt ttgttcggcg atattctgct tttcagacgg caaaacgctt
     9001 caatatttga agttcttacc cagataaaca gaacgaacct gttcgtttcc gaccaaatca
     9061 tcaggttttc ccgatgccag caccgcaccg tctgaaatga tgtaggcgcg gtcgcagatg
     9121 ctgagggttt cgcggacgtt gtggtcggta atcagtacgc cgatgccgcg cgatttgagg
     9181 aaaccgatga ttttctggat gtcgatgacg gcaatcggat cgacgccggc aaaaggttcg
     9241 tccaacaaaa taaaatgcgg tttcatggcg agtacgcggg cgatttcgac gcgtcggcgt
     9301 tcgccgccgg acagcgacgg cgcggggctg cggcgcaagt gtccgatatt aaggtcggcg
     9361 agcagttttt cgatttccct gtcgatttga tttttatctt tggttctgat ttccaagatg
     9421 gcgcggatgt tttgttcgac ggtcattttg cggaatatcg aggcttcctg cggcaggtag
     9481 ccgacaccga ggcgggcgcg ttcgtgtatg ggcaggtgac gcaattcctg cccgtccagc
     9541 atcacgctgc ccgcatcggc ggcaatgagt ccgactatca tgtagaagct ggtggtttta
     9601 cccgcaccgt tgggcccgag cagtccgatg acttcgccgc tttcgatttc gagggagaag
     9661 cttttaacga cttggcgttt tttaaaactt ttttgcaggt tttgaacaac aaggcggctg
     9721 acgtttgcgc tcataaatat ctcttcaata tcttcggtcg gcatatgccg tccgaaccgg
     9781 gtttatgttt cagacggcat tttgggatta ttcggatttt tgcgtactcg aaggctggat
     9841 aacgacgctg accctgccgg atttggaagc ggattttgcg ccggattttg tgctgccgct
     9901 gatggtatag acttcggttt tggtgttgta tgtaatcacc gcaccttcgg cgacatcgcc
     9961 gccgcgctgt actttggcat taccggttaa gactacggtg ctgcctgcag atgaataagc
    10021 aacgttgttt gcctgtccgc gcaccgtgcc tttgccgccg tccaatgtct ggctgaagcg
    10081 gactggcgaa ccttccgccc tcacggattc gccgcctttg ccgccgcgtg taacattgac
    10141 gcgggcggcg gaaatattga gcgtaccctg tctgatgacg acgtttccgc tgaatgtggt
    10201 gctttggttg gcttgatcga gcgaaccttg gtcggcctca atctgaatag gctgcctgct
    10261 gtcgctttga agggcaaaag cgggggacgc cgaaaaaaat gctattaaaa caaatagctt
    10321 acatatcttt tgtatcataa atcgtggctt tcactttaga tgagaagttc aacatgcctg
    10381 ttttgtggtc gtaagtcatg ccgcccgcct gaccgtgcga tgcaccatat tggaaactga
    10441 caggcgtatc ggtttgggca tattgagatt cggtatcgac gtgcagcttt tcggcttcaa
    10501 ctttacccgc ctgccgtttg ccgtcggcgg ttttggtcag cacaacgttg tttttaaaaa
    10561 gaacctgttt gttttcggta tggtaaacgg cttcgtcgct gccgacttcg tacaacaacc
    10621 tgccttcttg gaagaacacg agatgcggcg aatcaaaatg gatgtcgctg ctttcgggaa
    10681 actgtttcgc gcctttcgcg ctcaaatgtt cttttaagta tccctgttcg tcaaaccgcc
    10741 tgccatccaa tccgtccatt gtgtattgcg gttcgtcggg attgagcctg acttcttcaa
    10801 tctcgacttc gctgatgcgt cccaaccatg ccgacaggct gcccaaggca accgccaata
    10861 tcaatgggaa cgcaattccg taccgccatc ttattttcat ttgatgtact cgttcaaagc
    10921 cgcgcccaaa gtcccttgcg cctgcataat caggtcgcac acttcgcgca ccgcgcccgc
    10981 accgccgggg cgtttggcaa tataggcggc gtgttgcagc gtaaaccaat gcgcgtcggg
    11041 gacggcaacc ggcaatccgc aacgcaccat caccggcaaa tcgaccacgt cgtcgccgac
    11101 aaaggcacac tcggcttctt ccacgccgac ctgagcgcgc agctcttcat acgcggcgcg
    11161 tttgtccgaa atgcctttga agtagtaatt tatgcccaac tgtttgacgc ggatgccgac
    11221 ggagggcgcg tcccgacctg tgataatcgc agtctgcacg ccgcttgcct gaagcatttt
    11281 cagaccgtgt ccgtccagtg tgtgaaacga tttgatttct tcgccgttat cgcggataaa
    11341 gatgcgcccg tcggtcaaaa cgccgtccac atccaggatc agcagtttga ttttggcggc
    11401 gcgcgcctgt aattcgggag aaattgcctg catgggattt ccttgttttc agacggcata
    11461 tcttaccaca aatgccgtct gaaacggctg caacgcctta tacaatccgc gccgccaaaa
    11521 ggtcgtgcat attcagcgca ccgatcagca cgccgtctgc atcggtaacc agaagcccgt
    11581 tcacatggtt tgcctgcatg actttcaggg cttcggtggc gaggcgttcg gcggcgatgg
    11641 ttttagggtg tgtatgcatg acttcgtcta tcgaaagacc ggtaaaattg tcgcattctt
    11701 gaaacaggcg gcgcaaatcg ccgtcggtga atacgccttt cagacggcct tgcccgtccg
    11761 ttaccgccag catccccagc cctttttcac tcatgctgac gatggcttct ttcaagggcg
    11821 tgccgagtcg gacggcaggc aggccgccgc ctttgtgcat aatgtcggca acgcgcaaaa
    11881 gtaggcgttt gccgaggctg ccggcaggat ggctcaaggc gaaatcgtcg ggcgtgaacg
    11941 cgcgtgcgcg cagcaggacg accgccaacg catcgcccaa agccatgacg gcggtggtgc
    12001 tggtggtcgg ggcaagcccc agcgggcagg cttctttgga aaccgacgcc gtgatgtgga
    12061 tgtcggcatg gcgcgccatg gttgaatcgg ggcgggcggt gatgcagaca agcgtgatgt
    12121 ctttgcgttt gagcgcgggg atgatggcgg cgatttcgtc gctttcgccg gaattggaaa
    12181 tcgcgacgac cacgtcgttg tccacaatca tgcccaaatc gccgtgtgcc gcttccgcag
    12241 ggtggacgaa aaacgcgggc gtgccggtcg aggccatggt tgccgccatt ttgcgcccga
    12301 tatgtcccga cttgcccatg cccgtgataa caaccctgcc cttgcagtgc aacaacgcgt
    12361 ctgccgcaag gacgaagttt tcgtccaatt ccgctgcaat ttcgcgcaag ccttccgctt
    12421 cggtgtgcaa cacttcgcgc gcccagtcga gatattttcc gttttctgcc attgccatcc
    12481 gcctttgctg taacaaaact acccaaaatt tccgaatcgg ggtagcataa gcattcaaac
    12541 cccgccatcc atccaaagga atcagaaatg actattttat cggacgttaa agcattagga
    12601 caacaaatct ggctggacaa cctttcccgc tcgctcgtgc aaagcggcga attggcgcaa
    12661 atgctgaaac aaggcgtgtg cggcgtaact tccaatcccg ccatcttcca aaaagccttc
    12721 gccggcgacg cgctttacgc cgatgaaatc gccgccctca agcagcaaaa cctcagcccc
    12781 aaacaacgct acgaaaccat ggcggttgcc gatgtgcgtg ccgcctgcgg cgtttgcctt
    12841 gccgaacacg aatccaccgg cggcaaaacc ggtttcgtca gcctcgaagt ttcgcccgaa
    12901 ttgtccaaag acgcgcaagg cacggtagaa gaagcgcgcc gcctctacgc cgccatcggg
    12961 tgcaaaaacg cgatgattaa agtgcctgcc accgacgcag gcatcgatgc gctcgaaatc
    13021 ctcgtttcag acggcatcag cgtaaacctc acgctgctgt tctcacgcgc ccaaaccctc
    13081 aaagcctacg ccgcctacgc gcgcggcatt gccaaacgct tggcagccgg acaaagcgtt
    13141 gcccatatcc acgtcgtcgc cagcttcttc atctcccgtg tggacggcgc gctggacaca
    13201 acgctgcccg accacctcaa aggcaaaatc gccatcgccc ttgccaaagc cgcctatcaa
    13261 gattgggaac aatatttcac cacccccgaa tttgccgcac tggaagccca aggcgcaaac
    13321 cgcgtgcagc ttttatgggc atctaccggc gtgaaaaacc cagcctatcc cgacacgctc
    13381 tacgttgaca acctgatcgg cgcgcacacc gtcaacaccg ttcccgatgc cacgctcaaa
    13441 gcctttatcg accacggcac agccaaagcc acgctgaccg aaggcgtaga agaagcacaa
    13501 gcgcagcttg ccgaaaccgc cgcgctcggc atagacgtag aaaccctcgc caaccgcttg
    13561 caggaagacg gtttgaaaca atttgaagaa gcgtttgaaa aactgctcgc accgttggtt
    13621 taaacttttc atccccgatg ccgtctgaaa ggctttcaga cggcatcgcc cttttgaccg
    13681 gacacagtgc ggcatatcaa ggattcgccc taaaaatatt aatcaacaag aagttaatgc
    13741 acgacattac ccactaacac aaaaggaaat agaatcatgg caaaagcact cgaaatcatt
    13801 tcacccaatg aaatttattc agatctgatt tttaaggatc cggtacctcc ccatactata
    13861 tatagagaat tgatgaaact ggttagtaga aatgcaagta atgagagaat cgcagataga
    13921 gccttcgatt ttttctcgtc cggccttatg gatggttcag ctaccgaaga gcaatataat
    13981 gccctatatg atttaacctt gcttgaagag cccgggatgg aactgaataa agatgagata
    14041 atggctttga ttaatagtct taaataaagg ggctgtacca gatatgtaca caggacgttt
    14101 tgcccccagc ccgaccgggc tgctccacat cggctcgctg ctgaccgccg tcgcttccta
    14161 tgccgatgca cgctcaaacg gcggcaaatg gctggtccgc atggaagacc tcgatccgcc
    14221 gcgcgaaatg ccgggggcgg caagccatat cctgcacacg cttgaggcat tcggattcga
    14281 gtgggacgga gaagtcgcct atcagagccg ccgttacgcc ctgtatgaag aaacactatg
    14341 ccgcctgaaa accgccggac tggtctatcc ctgccattgc agccgcaaag actggcagac
    14401 cggggcaagg cggggcgcag acgggttcgt ctataacgga cgctgccgca atccgcagca
    14461 acgccctgca ctgcaaggca aacagccggc gtggcgcatc cgcgtccccg accgcgacat
    14521 cggcttttca gacggcatcg tcggcggtta cgcccaaaac ctcgcccgcg acatcggcga
    14581 tttcgtcctg cttcgtgcag acggttactg ggcataccaa ctcgccgtcg ttgccgacga
    14641 tgccgaacag ggcgttaccc acatcgtccg cggacaagac ctgcttgttt ctacgccgcg
    14701 ccaaatctat ttgcagcagt gtttggacgt tccgacaccg caatatgccc acctgccgct
    14761 gctgaccaac gcacaagggc agaaatggtc gaaacagacg ctcgcacccg cattggattt
    14821 aaaccgccgc gaacaactcc tccgccaagt gttccgttac ctcaacctgc ccgaagcacc
    14881 ggaagccgac cgccctgccg aactgctcga ctgggcggtg gcacactggg atatgggcaa
    14941 agtgccgaaa cacgccatta ccgcccccta aacaccgccc gacaaacgca caaatgccgt
    15001 ctgaaacctt cagacggcat ttcatacaca cagccccgcc ctattcgcgc atatttgccc
    15061 gttcgacttc tttccacgct tcgagaatca ggccgccgtc gttgtctttc aagagcgttg
    15121 catcggaaag catacgccgc caagtgcggg catttttcag accgtgcatc agcccgaggc
    15181 tgtggcggac gatgtggcgc aagattgtgc cgcgtccggc ttggatttgg gcttgactgt
    15241 atgtgtagag acgctgcacc aaatcggcgt attcaatcgg gctgcgggta tcgccgtaaa
    15301 acagcctgtc ccattcgcgc atcaccatcg ggttgtggta cgcctcgcgc ccgaccatca
    15361 cgccgtcaac gtgttgcagg tgttgcgcga tttcctcatt ggtcgtgatg ccgccgttga
    15421 tgatgatttc cagcccggga aactcttgct tgaggcggta aacataatcg tatttcaagg
    15481 gcggaacgtc gcggttttct ttgggtgaaa gaccgtccag ccacgcgtta cgtgcgtgga
    15541 cgataaaggt tttgcaggcg gttttgtcgc gcagcgtgcc gacgaaatcg gcaacggttt
    15601 ggtattcggt ctgcctgtcc acaccgatgc ggtgtttgac ggtaacggga atcttgaccg
    15661 catcctgcat ggcgttgagg cagtcggcaa ccagcccgac ttcgttcatc agacacgcgc
    15721 cgaacgcgcc tttctggacg cgcggactgg ggcagccgca gttgaggttg acctcgttgt
    15781 agccgtattc ttcggcggct ttggcggctt tcgccaaatc ggacgggtcg ctgccgccca
    15841 gttgcagggc gacgggctgc tcgccttcgt taaacatcaa aaagcggtct ttgtcgccgt
    15901 aaacaatcgc gccggcgttg accatttcgc tgtacagcca agtattgcgg gtaatctggc
    15961 gcgccatata acggtagtgc ctgtccgtcc agtccaacat cggggcgacg gacaggcggc
    16021 ggggaggaag ggtttgcgtg ttgtcgttca ttgtcgatat ttcaccattg cggattcaat
    16081 ttcaatccgg aacaaccttc atttttgggg aaatccgcgt catgaaagag tccagcagta
    16141 tggttaccgg cccgtcgttg cagagcgaca cctgcatatg cgtgcggaaa cgccctgttt
    16201 cgacatgaat tccgtgtccg cgcaacagtt ccgccgttcg caggtaaagc tgctgcgcct
    16261 gttctgcagg tgcggcttgc gaaaacgaag gccgccgccc gcttgtcgcg tcggcataaa
    16321 gcgtaaactg cgacaccagc agcaccgcgc cgccgacatc tttcaaagac aggttcagct
    16381 tgcccgcttc gtcttcaaac acgcgcaaat gggcgatttt gtcggcgata tagcgcgcat
    16441 ctttttctgt gtcactatgc gttacgccga gcagcacgac gaatccgccg tcgattttgc
    16501 cacaggtttc cgtgccgttt tcggacacga catccacctt tgcacctacc gttttctgta
    16561 tgaccgcacg cataagccgt cctccttacc tgaacgtgca atgccgtctg aaaaggcttc
    16621 agacggcatc ggtattggaa atgccattac ggtttgacac cgttttcttc caaaatggca
    16681 tcgattttca agcgggcttt ttcctgtctc aaaccttgtt ccaactggtt tctgaccaac
    16741 tcgaaaggct gcgcgtcggg gtttttcccg acctcgctga gtttgaacag ataatagcgt
    16801 tcgcccaatt tgaccggatc gcgggtaacg tcgccccgat tcatcgcggc aaactgcgaa
    16861 gccagcggct cgggaagctg ctgcgccata atgaaaccgt caaaagcctg ctcgtcgttc
    16921 ggataacgct tcatcagccc ttcaaaagac agccctttga gcaggagctg ctgcgcctga
    16981 cgcgcctcct cttcggttgc gaagctgacc tgctgcaatt tgatcatgcg gatttgccgc
    17041 tcataaaact gacgcagtgc gctttcggaa accgtttccg aacgttccag aaaacggacg
    17101 tactcctcgg cataaaaaga cgcttcggcg attttaaagc ggttttggac atccttatcc
    17161 ttatccaaac cttccttcaa tgccctgttt ttcaaaactt ccaaagtttg cagccgacgg
    17221 acggcatcgt ttcggattgc ctgcccgtcc ggtttttggg actgctccgc atgccggtct
    17281 gcctgctgca tgatctgcgc caccagcgta tccaccaaag ccgggtcgat ttcgggtgct
    17341 ttggctgccg caaaacctgc caacattgca gcaataactg cggcagcggt ttttttctgt
    17401 ttcatggtat tccctgtcct gcgcggcggc aaaacgcctg tctgaacgtc ttgccgccat
    17461 atcccgtttt cagaattatt ttgcaggttt gatgtttgcc ttgcccaaca gtgcaccgac
    17521 ggcacggtca atccgttccg cctgaaggtt gccggcaatc tgtcctttca tttcatcaaa
    17581 agaaggcact tttacctcgc ggctgtcgtt gacataataa acgccgtaga aatcgccgtt
    17641 tttcagcggc gttgccgtaa attcgccttt tttcaagtcc ttaattgcct gataaagcgg
    17701 cggaacaccc tgttccaaat ctttcagcgg cacatatccg accggcgcac cggtctgttt
    17761 ggtacggtcg ttgagggaat attgtttcaa gacggcatcg aaaccttttt tcgccttcaa
    17821 gtcggcaacc gctttttttg cattttcttc cttgtcggtc aggatttcgc ccaactggac
    17881 ttcctgcgta cctttgtaaa aaccgctgat attgtcatat gcggctttta tttcctgctc
    17941 ggaaaccggt tgggttttgg cgatatgcag cgcgtatgcc tcgccgttca agccatattt
    18001 taccgcctgc caaacggttt tgaaggacgg tttcttgtcg tcgcccgact ttttcgcttc
    18061 ggcacgcaat ttggcaagcg catttttaaa ctctgccgac cggtcgagtt tcaggcgttt
    18121 cacttcctgt gcgaccacgg tgttgaccac ttcgttttcc agcagggatt ggcgcagttg
    18181 cggcgtgtct tcggcacggc tgttttccgc acggaatgcg gcaacctgcg catcgatgac
    18241 ggaactgtcg attttctgac cgttgacggt tgccagcgtt tgggcaaaca ggctgccgga
    18301 acaggcaagc agtgcaacgg aagtcaggat ttttgctttc ataatcttct ctctttaaat
    18361 tttaaattga aatgttcggg cagacttcat tcagtccgcc gtatggaaat actcgtcagg
    18421 ggtagccgcc cggatgccga gcgcgtgaat catgccgcct gaaaacaaat ctttgagcag
    18481 cgatttgacc gttttctggc ggttcaggcg gcttacgcct tcaaaacggc cgctaacgac
    18541 caaaacggca taatgtccgc cgccggtatt gcccgcgtgt cctttgtgca gatggctctc
    18601 atcgccgatt tccaacacca gcggatcgag cgtctgcagg cgttcgcgga tcaaatcgac
    18661 ggccggcatt atttgaacac cgctttaaac ggcttgatca gcacttcgct gtacacgcct
    18721 gcatgaacat agggatcgtc ttccgcccac gcctctgccg catccaaaga ctcgaactgc
    18781 gccacaatca aactgcccga aacgcggttg gagtcctccg gcaaaggatt cgggcctgcc
    18841 gtcaacagcc ggccttccga cttcagcgtc tccagccgtt tgaggtgttc gggacgtgcc
    18901 gccatacgcg cttcatacac attctccccg tctgttgcca gcaacataaa atattccaca
    18961 gtcaatcctc ctttttcaga caggtactca gataaatacc ctgaataata aagaaaacaa
    19021 gcatcagtgc agtcgagccg aacattttat agttgaccca ttgcgactcg aaccgggtaa
    19081 acacaaacca gttggcgata cccataaaaa tcaggaaacc gacccacata tacgtcaatt
    19141 tcgcccatac ggcatccgga agctgaatct ccctgccgat actcgccttc aagccgtttt
    19201 taccggcgag gtggctgccc cacaggaaca gcgcgcccag ccaaaacaaa acgctcggct
    19261 tccacataat gaagcggctg tcgcccaaaa caatggttgc gccgccgaat acgacaatca
    19321 gcaccagtcc gacccactgc atcgtatcca gctttttata tttccaatac agaaaagccg
    19381 cctgaaccac accggcaacc aaggcaaccg ccgttgcggt aatcatgttt ttggtaacgg
    19441 tataagtggc gaaaaacagg atgacggaaa aaaggtcgct gacaaatttc atatccaaat
    19501 gagaaagcaa atcaaataaa cgctaatcat acaggcagaa ccctgttttt gcacctgcaa
    19561 aaccgacaaa ttttatttcg gacgatgcaa atatgcacag atgtttttga aaaaagatga
    19621 agatatgaca taatttgtaa aattggcttt aaaatacagc ttcggccggg acctctgcct
    19681 atccgcaccc tatcgtccgt attgccgatg cctgatgacg aaatttacag actgtacgcg
    19741 gtcaaaccgt attcagccgc caacccacag gggatacatc ttgaaaaaca acagacaaat
    19801 caaactgatt gccgcctccg tcgcacttgc cgcatccttt caggcacatg cgggactggg
    19861 cggactgaat atccggtcca accttgacga acccttttcc ggcagcatta ccgtaaccgg
    19921 cgaagaagcc aaagccctgc taggcggcgg cagcgttacc gtttccgaaa aaggcctgac
    19981 cgccaaagtc cacaagttgg gcgacaaagc cgtcattgcc gtttcttccg cacaggcagt
    20041 ccgcgatccc gtcctggtgt tccgcatcgg tgcaggcgcg caggtacgcg aatacaccgc
    20101 catcctcgat cctgtcggct actcgcccaa aaccaaatct gcactttcag acggcaagac
    20161 acaccgcaaa accgctccga cagcagagtc ccaagaaaat caaaacgcca aagccctccg
    20221 caaaaccgat aaaaaagaca gcgcgaacgc agccgtcaaa ccggcgcata acggcaaaac
    20281 ccataccgtc cgcaaaggcg aaacgcttaa acagattgcc gccgccatcc gtccgaaaca
    20341 cctgacgctc gaacaggttg ccgatgcgct gctgaaggca aacccaaatg tttccgcaca
    20401 cggcagactg cgtgcgggca gcgtgcttca cattccgaat ctgaacagga tcaaagcgga
    20461 acaacccaaa ccgcaaacgg cgaaacccaa agccgaaacc gcatccatgc cgtccgaacc
    20521 gtccaaacag gcaacggtag agaaaccggt tgaaaaacct gaagcaaaag ttgccgcgcc
    20581 cgaagcaaaa gcggaaaaac cggccgttcg acccgaacct gtacccgctg caaatactgc
    20641 cgcatcggaa accgctgccg aatccgcccc ccaagaagcc gccgcttctg ccatcgacac
    20701 gccgaccgac gaaaccggta acgccgtttc cgaacctgtc gaacaggttt ctgccgaaga
    20761 agaaaccgaa agcggactgt ttgacggtct gttcggcggt tcgtacacct tgctgcttgc
    20821 cggcggaggc gcggcattaa tcgccctgct gctgcttttg cgccttgccc aatccaaacg
    20881 cgcgcgccgt accgaagaat ccgtccctga ggaagagcct gaccttgacg acgcggcaga
    20941 cgacggcata gaaatcacct ttgccgaagt cgaaactccg gcaacgcccg aacccgctcc
    21001 gaaaaacgat gtaaacgaca cacttgcctt agatggggaa tctgaagaag agttatcggc
    21061 aaaacaaacg ttcgatgtcg aaaccgatac gccttccaac cgcatcgact tggatttcga
    21121 cagcctggca gccgcgcaaa acggcatttt atccggcgca cttacgcagg atgaagaaac
    21181 ccaaaaacgc gcggatgccg attggaacgc catcgaatcc acagacagcg tgtacgagcc
    21241 cgagaccttc aacccgtaca accctgtcga aatcgtcatc gacacgcccg aaccggaatc
    21301 tgtcgcccaa actgccgaaa acaaaccgga aaccgtcgat accgatttct ccgacaacct
    21361 gccctcaaac aaccatatcg gcacagaaga aacagcttcc gcaaaacctg cctcaccctc
    21421 cggactggca ggcttcctga aggcttcctc gcccgaaacc atcttggaaa aaacagttgc
    21481 cgaagtccaa acaccggaag agttgcacga tttcctgaaa gtgtacgaaa ccgatgccgc
    21541 agacgatttg tccgcattgc ttcaacctgc cgaagcactg tccgttgagg aaaatataac
    21601 ggaaaccgtt gccgaaacac ccgacttcaa cgccaccgca gacgatttgt ccgcattact
    21661 tcaaccttct gaagtacctg ccgttgagga aaatgcagcg gaaaccgttg ccgatgattt
    21721 gtccgcactg ttgcaacctg ctgaagcacc ggccgttgag gaaaatgtaa cggaaaccgt
    21781 tgccgaaaca cccgatttca acgccaccgc agacgatttg tccgcattac ttcaacctgc
    21841 tgaagtacct gccgttgagg aaaatgcagc ggaaatcact ttggaaacgc ctgattccaa
    21901 cacctctgag gcagacgctt tgcccgactt cctgaaagac ggcgaggagg aaacggtaga
    21961 ttggagcatc tacctctcgg aagaaaatat cccaaataat gcagatacca gtttcccttc
    22021 ggaatctgta ggttctgacg cgccttccga agcgaaatac gaccttgccg aaatgtatct
    22081 cgaaatcggc gaccgcgatg ccgctgccga gacagtgcag aaattgctgg aagaagcgga
    22141 aggcgacgta ctcaaacgtg cccaagcatt ggcgcaggaa ttgggtattt gattcccaac
    22201 tgccctttcg cagatcaagg atgccgtttc agacggcatc tttttttgcc ttatcggtgt
    22261 aacggataaa gtttgaaccg gcacaggctc aaacagcagg tcgacggcaa caaaatgccg
    22321 tctgaaaccc ctaaaggctt cagacggcat tggcggcggc ggtatcaggc agtttgatag
    22381 gcaaccgaat cgtcaccgct tttcttttga atgaactcga ttttgtatcc gtcggggtct
    22441 tcgacgaagg ctatcacggt tgtgccgtgt ttcatcgggc cggcttcgcg gacgacgttt
    22501 ccgccctgcc gcttcacacg ttcgcaggct tcgtaggcat cgtccacttc aaccgcgatg
    22561 tgtccgtagg cgttgcccaa gtcgtatcgt tccgtatccc agttgtgcgt cagttccaaa
    22621 accgtgctgt cggtttcatc gccgtaaccg acgaaggcaa gggtaaatct gccttcggga
    22681 taatcttttc ggcggagcag tttcataccc aaaacgtttt ggtagaaatc gagggatttt
    22741 tcgagattgc ccacgcggag catagtatgg agtaagcgca ttttttgtgt tcctttcggt
    22801 ggtggttaaa cttcgatttt attcggggta aacgtctgcc atttgttgca ggcggggcag
    22861 tgccagaaaa agacttggga tttgaagtgg cagttgcggc aacggtacat cacgctgcgc
    22921 tgtagctgcc gtccgataac cgaacgcatc atgtcggcat cggctttcca agccggattc
    22981 atatcgctga gtttcaaacc gagcaggcgg tacacgccgt taaggtcggg cttgcggcgg
    23041 acaagctcga cggcggtttg cgcggcttct ttctcgccct taagcagcag ggatttctcg
    23101 tacacgacat tgatcaggtc aagttcggga aacgtctgca tatatcctgt cagacggttc
    23161 aagccttctt caggttttcc ctgcgcggca taggcttcgt aaagcttctc gccgaccatg
    23221 ctcaagtatg catggttttg ctgctcgatg gcggcatagg cttcgacggc ggcagggaaa
    23281 ttgccttgtc ggtgttcgat gtcgcccaaa atcatgttgg cgcgggtgca ttttttgttg
    23341 gcttcgagtg ccttgccgac attgaaacgc gcggcatcga aattggactt gaacagcgcg
    23401 gcttgggcaa gttcgcaata gaactgtgca atctcaaact gataggtctg ctcgtcatgg
    23461 ctgagcagcc gggcggtttc aaccgctttt tcccaatccc tgtcctgttg gtagatattg
    23521 agcaggtgct gtctggcttc acgcgccatt ttaccgtctt gcagccccaa aaaaatctgt
    23581 tcggcacgat cgaccaaccc cgcactttgg tagttttgcg ccaattcaaa caggacgcgc
    23641 gcgcgcttgg ctccggttgt atcgggagag tcaagcaatg tttggtgcat attgatggct
    23701 ttgtcgtttt cgccacgctg acggtaaagc ttgcctaggg tgaggttcag gtcgtatgac
    23761 tgaggctgct gatcgatgac ctctgccaat tccctggcgg cacgtccgct gttgcggtca
    23821 accaaggcat ccagactttt ataaaatccc gacggtatgc tttttgcctg cttcaatacg
    23881 gttttcatat ccacgcgggc ggcaaaccag cccatcgcga agaagacggg caaaaggata
    23941 atcggcagca ggataatcca caattcgttg tccatatcgg ctttcttaag gctgtttggt
    24001 agattcgggc gcattttgcg ccggtggtgc ggtcagctcc ttccccgtca aacgcgcatt
    24061 tttctttact tcggcacgca acctgccgtt ctcgccacgt aacgacaaca accgtccgaa
    24121 caaggcaaac attccaaaaa taataccgac tacaaatgcg ccgaacaata cgacaatcag
    24181 cggcaaatcg aatttttgcc ccggcaggta ggaaaaggta acggcatccg tattaatgac
    24241 ggcaagcagc aggaagagca gcaggataat gattttgatg acggtataga taagtttcat
    24301 gtttgctccg taaataaaaa cgccgcccgg acagcttcgc actctgaacc aagattgcgc
    24361 tagtttaaac gatttgcacg gtttcggata ggatgcggca gcgtgttcgg acgacatacg
    24421 gagtatgcga ttgccgacaa ttttaccaaa tacaccgttc ctttccattt gaaaaataac
    24481 ggattggaca ccgcatcgac agaaaaaccc gccgcgcact tgtcaaaacc ctgtttgcag
    24541 gcgtatcttt acaatcttca aattcaaacc gttcattgaa acatatcaga ataagaaagg
    24601 ctttacatca tgagcagacc cgtacccgcc gtattcggca gcgtttttca cagtcaaatg
    24661 cccgtcctct cctaccgcga aggcaaatgg cagccgaccg aatggcaatc ttcccaagac
    24721 ctctccctcg caccgggtgc gcacgccctg cactacggca gcgaatgttt cgagggactg
    24781 aaagccttcc gtcaggcaga cggcaaaatc gtgctgttcc gtccgactgc caatatcgcg
    24841 cgtatgcggc aaagtgcgga cattttgcac ctgccccgcc ccgaaaccca agcctatctc
    24901 gatgctttgg tcgaactggt caaacgtgcc gccgatgaaa ttcccgacgc gcctgccgcc
    24961 ctgtacctgc gcccgacctt aatcggtacc gatcccgtca tcggcaaggc aggctcgccg
    25021 tccgaaaccg ccctgctgta tattttggct tcccccgtcg gcgactattt caaagtcggc
    25081 tcgcccgtta aaatcttggt ggaaaccgaa catatccgct gcgccccgca catgggccgc
    25141 gtcaaatgcg gcggcaacta cgcttctgcc atgcactggg tgctgaaggc gaaagccgaa
    25201 tacggcgcaa atcaagtcct gttctgcccg aacggcgacg tgcaggaaac cggcgcgtcc
    25261 aactttatcc tgattaacgg cgatgaaatc attaccaaac cgctgaccga cgaatttttg
    25321 cacggcgtaa cccgcgattc cgtactgacg gttgccaaag atttgggcta taccgtcagc
    25381 gaacgcaatt tcacggttga cgaactcaaa gctgcggtgg aaaacggtgc ggaggcgatt
    25441 ctgaccggta cggcagccgt catctcgccc gttacttcct tcgtcatcgg cggcaaagaa
    25501 atcgaagtga aaagccaaga acgcggctat gccatccgta aggcgattac cgacatccag
    25561 tatggtttgg cggaagacaa atacggctgg ctggttgaag tgtgctgatg ctttaaataa
    25621 aaaatgccgt ctgaaacccg tttggcgttt cagacggcat tttcgcatcc gaaccgtttc
    25681 cgctgcacct gcagcaagtc ggcacaaagg caatcggtta aaacaagcgt ccgcatttcc
    25741 catcccgcct gccgtaagtc gggcatttcc ctagaaatac gcttcagacg gcaaaacgcc
    25801 gcccgaaacc gatatgcggc acggacggcg cacggatttg aaaacggcag attatccctc
    25861 ggtgctcaag gcattgatgc tgtaaccgcc gtcaacgtaa gtgatttcgc cggtaatgcc
    25921 ggacgacagg tcggacagca ggaaggcggc ggtattgccg acttcttcga tggtaacgtt
    25981 gcggcggagc ggattgtggg cggcgacgtg tcccaagagt ttgccgaaat cggcgatgcc
    26041 ggaggcggca agcgttttga tcgggcctgc ggaaatgccg ttgcagcgga tgccttcttt
    26101 gcccagacag gcggcggtaa aacggatgcc cgcctcaagg ctggcttttg ccatacccat
    26161 cacgttgtaa ttcggaatcg cgcgcaccgc gcccaagtag ctcagggcaa cgatggcgga
    26221 gtttctgccg cgcatcatcg gacgggcggc ttttgccaat gcgggcaggc tgtatgcgga
    26281 aatttcgtgt gcggtgttga acgcttcgcg gctgatgctg tcgaggaagt cgccgctcaa
    26341 ggcttctttc ggcgcaaaac cgatggaatg caccaaaccg tccaagccgt cccaatgttt
    26401 gcccaaatcg gcgaacactt ggttgatttc gtcgtcgctg gcgacatcgc agcggaacac
    26461 gagttcggaa tccaattccg ccgccatttt gcggacgcgc tcttccagtt tgtccacaac
    26521 gtaggtaaac gccagttccg cgccttgttc gcggcaggct ttggcgatgc cgtaagcgat
    26581 ggaacgctcg gaaatcatgc cggtaatcag aatttttttg ccttgcagaa aacccatttt
    26641 gtatccttca aatagtgttc ggttcgttct aaaccgttga ttatagcaaa ttgtccctgt
    26701 ttctgtgttt tcacgttgca gcatgcaaac ggcaatgccg tccgaagcgg atttcagacg
    26761 gcatcggacg tttcaaatac ggtttaaggc atcagatgcc gcgcaacaat tcgttcacgc
    26821 tggttttcgc acgggtttgc gcgtccacgc gtttgacgat gacggcgcag taaaggctgt
    26881 ggctgccgtc tttggaaggc atactgccgg acacgacgac cgaacctgtc ggtacgcggc
    26941 cttggtagat ttcgccggtg gtgcggtcga agattttggt ggattgaccg atgaacacgc
    27001 ccatcgaaat cacgctgcct tcttcgacaa tcacgccctc aacgatttca gaacgcgcac
    27061 cgatgaagca gttgtcttca atgatggtgg gggcggcctg caggggttcg agtacgccgc
    27121 cgataccgac gccgccactc aagtgcacgt ttttaccgat ttgcgcgcaa gagccgacag
    27181 tcgcccaagt atcaaccatc gcgccttcgt cgacgtatgc gccgatgttg acgtaagacg
    27241 gcatcagcac gacatttttc gccacaaagc tgccgcgtcg ggcaaccgca cccggaacgg
    27301 cgcggaagcc tgcgtttttg aactcgtctt cagaccagtc ggcaaacttg gtcggcactt
    27361 tgtcgaagta tttgttcacg ccgtcgttga ggacttcgtt gtcttggatg cggaaggaca
    27421 gcaacacggc ttttttcgcc cattcgttga ctttccactc acccacgccc aagcgttcgg
    27481 caacgcgcag tttgcctgaa tcgagttggc ggatggtttc caacacggct tctttgactt
    27541 cgggagtaac ggtggtcggg gtaatgtccg cgcggttttc aaaggcggtt tcgataatgt
    27601 tttgcaaaga cataatgttt ccttatgtga gatgtttcgt gggacgcttg ccggtatttt
    27661 caagcagtat gttcatttga aatgaatgcg ctattgtgcc acgcaaacgc atattcgtca
    27721 gcaatacacg ggcggttgcc gaacattggc gttccggaat cgggcgggcg gcgggcattg
    27781 ctttttccca cgcccgaatc aatacagaac aacggcagtg cctttatacc gcgccgccgc
    27841 ctttcagacg gcattcgcgg taaaacgcca tcagcccttc ggtcgaggca tcgtgtacgg
    27901 acgtgccgcc ttccagttca ccgatgatgg tttttgccaa ctgtttgccg tactcaaccc
    27961 cccactgatc aaagggattg atgttccaga ttacaccttg tacaaaagtt ttgtgctcgt
    28021 aagccgccat cagcataccc agattgtagg gcgtgaggcg gtcgagcagg atgctgttgc
    28081 tggggcggtt gccggggaac tctttgtgcg gcgcgaggcg ttcgcgttcc gcttcgggca
    28141 aatctgccag ttcggcgcgt gcttcgtcca aggttttgcc cttcatcagg gcttccgctt
    28201 gggcaaaggc gttggcaacg gtaaaacggc tgcgtccgtc ctccacgccc tgcgccgtca
    28261 tcgggacgat aaagtcgcag ggaatcaggc gcgtgccttg atgaagcagt tggaaatagg
    28321 cgtgctggca gttgacccct tcaccgccga acacgatgcc gcccgttttg cacacggcgg
    28381 gactgccgtc tgaagcgcgg cttttgccca aactctccat atcgagctgg ttcagccaca
    28441 ccggcagcag gcgcaggttg tggctgtacg gaacggcggt ctgcccgtcc gcgtgttgga
    28501 aattgttata ccacacggca atcagtgcca tcagaacggg aatgttgcgg cgcggcggcg
    28561 tgtggaaaaa atggctgtcc atcgcgtgcg cccccgccaa caactcgcgg aaacgcgccc
    28621 caccgaccgc aaccatcacg ggcaaaccga cgggcgacca gacggaatag cgtccgccca
    28681 cccagtcgta catcgcaaac acgcgttccg ccgcgatacc aaaagccgca gctgccgcag
    28741 tgtcggcaga caccgcgcaa aaatggcgcg tcgtttcgga ttccgagaac cccgcaccgc
    28801 gataccacgc cttgactgcc tgtgcattga gcagggtttc cggtgttttg aaggacttgc
    28861 tggcaacgca aaacactgtc gtttcggggt tcagacggca taaaaccgca tccaggcagg
    28921 caggatcggc gttggcggca aaatggacgg cgatatgccg tctgaacggc tcgagtgcct
    28981 gcacgcacat tgccggcccg aggtcggatc cgcctatgcc gatgtggaca aaatccgtaa
    29041 tccgtttccc agttatcccc tgatacgaac cgtcgtccaa actgtgtgca aacttcaacg
    29101 cacgatttaa ctcgcggcgg atttcgggca acacgtccct gccgtccaca taaacggcat
    29161 ccgcaccgtc gggcaggcgc aaagccgtat gcagcgcagc acgcccctcg ctgttgttga
    29221 ccttcgcacc cgtccacaaa tcatgcattt tccgtccgag ccctgccgca tctgcaaggt
    29281 tacagagcag ttgaagcgta tcttccccaa gccggttctt gctgtaatcg aacaacatgc
    29341 cgtccaaacg ttcgtgcata cggtcaaagc ggtccggttc gcaggcaaag cggtcacgca
    29401 aaagaatatg tcgcgtatcc tgacaatgtt gttcaagctc ccgccacgcc cgggtaaaag
    29461 cattcatctg ttttccttga tttttcagaa ccggattaaa atgtagcaga atgtagttta
    29521 acaaacggca gcggctttgg cgaatctccg gaacaccgcc cgccgcgtcc aatagcacct
    29581 tatttccata tggcgaaaat atagtgaatt aacaaaaatc aggacaaggc ggcgagccgc
    29641 aaacagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt
    29701 ctctcttgag ctaaggcgag ccaacgctgt accggttttt gttaattcac tataccctcc
    29761 ggccggcatt tgacccgcaa caatatcctg caagatttat tgtgtacgca taaatgccgg
    29821 acagccgcct aaataaggta taatcgcatc cgattctgtc ccgtttgccg cccgcaggcg
    29881 ggatgtcggc ggttttcatc ttaccgggaa cacgccggta ctgtttttta acgatgtttc
    29941 ttacatttta ttccaattac tttacggggc tagaatatgg ctaaaaacgg aggattttct
    30001 ttgttcgcaa agaaagaaaa acgctttatc tttgaaggca ggcattccgc ctccgacaaa
    30061 ctggtcaacg gcgaagtatc cgcgtttacc gaagaagagg cgcgcaaaaa actggcaaaa
    30121 cgcggcatcc gcccgttgca gattacccgt gtgaaaacaa gctccaagcg caaaatcaca
    30181 caagaagaca tcaccgtttt cacccgccag ctgtccacga tgattaaagc gggcctgccg
    30241 ctgatgcagg catttgaaat cgtggcgcgc ggacacggca acccgtctat gacggaaatg
    30301 ctgatggaaa tccgaggcga agtggaacag ggcagctcgt tgagccgcgc attctcaaat
    30361 cacccgaaat atttcgaccg cttctactgc aacctggttg cggcgggcga aacgggcggc
    30421 gtattggaaa gcctgctgga caaattggca atttacaaag aaaaaaccca ggccatccgc
    30481 aaaaaggtaa aaaccgcact gacctatccg gtatccgtca tcgccgtcgc catcggtttg
    30541 gtattcgtga tgatgatttt cgtactgccc gcctttaaag aagtttacgc caatatgggc
    30601 gcggagcttc ccgcactgac ccaaacagtg atggatatgt ccgacttttt cgtctcatac
    30661 ggctggatgg tgctgatcgc actgggcttt gccatatacg gcttccttaa attgaaggcg
    30721 cgttcgatta aaatccaacg gcgtatggat gccatactgc tgcgtatgcc gattttcgga
    30781 gacattgtcc gcaaagggac gattgcccgc tggggcagga cgacggcgac gctgattgcg
    30841 gcaggcgtgc ctttggtcga tgtattggac tccactgccg gcgcggcggg caatttaatc
    30901 tatgaagaag ccacccggga aatccgtacg cgcgtcatcc aaggtttgtc tatgacttcg
    30961 gggatgcgtg cgacggaact gttccccaat atgatgttgc agatgtcttc catcggcgag
    31021 gaatcgggtt ctttggacga tatgctcaac aaagccgccg aattttacga agacgaggtg
    31081 gacaatgcgg tcggccgact gtccgccatg atggagccga tcattatcgt gattttaggc
    31141 ttggtcatcg gcacgcttct ggtcgccatg tatctgccgc tgtttaattt gggcaacgtg
    31201 gtcgcctgat ttgccgcaca gatccggcgc ggattcgctc tgcgccggtt tgtttttgtt
    31261 ttgaatacat tgagaacaaa atatgtctga tttgtctgta ttgtcgccgt ttgccgtgcc
    31321 tttgacagcg gtgttcgggc tgctggtcgg aagtttctta aatgtcgtca tttaccgcgt
    31381 gccggtcatg atggaacgcg gctggacggt atttgccaaa gaatatttaa acctgccgct
    31441 gaccgaagag gaaagccgta ccttcaacct gatgaaaccg gattcctgct gtcccaaatg
    31501 ccgcgtgccg atacgcgcgt ggcagaacat cccgattgtc agctacctgc tcctgcgcgg
    31561 caaatgcgcc tcctgccaaa ccaaaatcag catacgctat cccttaatcg aactgctgac
    31621 cggcgtattg ttcgggctgg ttgcctggca atacggctgg tcttggatta cattgggcgg
    31681 tttgatactg accgcgtttc tgatttccct gacctttatc gatgcggaca cccaatacct
    31741 gcccgactcg atgacactgc ccttgatttg gctggggctg atatttaatt tggacggcgg
    31801 cttcgtgcct ttgcagtctg ccgttttagg tgcggttgcc ggctatggtt cgatatggct
    31861 tttatgcgcg gtatataaac tgattaaagg cgaaatcggt atgggcaacg gagatttcaa
    31921 actgattgcc gcattgggcg cgtggctcgg catatccgca ttgcccgtgc tgatttttgt
    31981 ttcctcgctg atcggtttgg tcgcggcaat cgttatgcgc gtcgccaagg ggcagcattt
    32041 tgccttcggc cccgcactga cagtttcggg ctggataatt tttacggcaa acgattccgt
    32101 atggcgggcg gtcaactggt ggctgaccca tccggtgctg taagatgacg gtatgggtcg
    32161 ggctgaccgg cggaatcggc agcggcaaat cggcagccgc gcaatgtttt gccgatttgg
    32221 gcgtgccgcg tatcgatgcg gatgcggcgg cgcactcgct gacggcttca gacggcatcg
    32281 ccctgccgga aatcaggcgg ctgttcggcg acaccgtttt cgacacacag ggtttgttgc
    32341 gacgcgacat attgcgtaaa gaagtctttg cctccccatc gcgaaaagcc ttgctcgaat
    32401 ccgtgatgtt gccgctgatt ttctcagaaa tcaaaaaaca gcaagaaacc tttaccgatg
    32461 cggcttacgg cattgtcgaa attccgctgc tgacggaaaa gcgtcaattt atcatcctga
    32521 tacggcgtgt cctgaccata agtgcccctg tggaaaaacg tatcggcagg gtgatggccc
    32581 gcagcgggct gacgcgcggc gaggtggctg ccgtcatcag ccatcaggca tccgaatccg
    32641 aacgcctgct gcttgcagac gatgtgctgc tcaatgacgg cagcctcaaa agcctgcgtg
    32701 agaaaacaat gcgcctgcac gcgttttatt cagggatttt cgcctcaaaa ccaacacaag
    32761 gaaaacacaa tgactgaatc gcggcaaaca cgccttcaag tcaaatgtcc gacctgtcaa
    32821 acgacagtag tatggaaacc cgaaaacgca ttccgcccgt tctgttcgca acgctgcaaa
    32881 ctgatcgact tgggcggatg ggcagacggg aaatatacgg tttccgacca aacggaaagt
    32941 ttgctggaaa tatccgaacc ggacggcgca taccgctgac cgcaccccgc cctccggcaa
    33001 acccctgaaa atcaaatgcc gtctgaaaca aacacgcttc agacggcatt ttcattccca
    33061 agcctaatcg ttggtatttg ccgttacctc ttccaacgag gtaatgccct gcataacttt
    33121 caaaataccg gcccggcgca aatccaccat accctcctta taggcaacgt ccaaaatatc
    33181 cacttccgta ccgttgttca taatcacgcg ctgcatttct tcgctgatgg gcataacctc
    33241 atacacgccc gcacgccctt tataaccctg cccccggcaa cggtcgcaac cgacggcgcg
    33301 gtaaagtttc caatcttttg caaggtcctc atcggtgaag ccgacttcct tcaaagcaga
    33361 ggcagacggg cgttccactt cctgtttgca gctcgaacac agcctgcgta aaagacgctg
    33421 cgccataatc aggctgaccg aactggcaat attaaacggc gcgacaccca tattcagcat
    33481 acgcgacaac gtcgccggcg cattattggt gtgcagggtg gaaaacacca tatgccctgt
    33541 ttgtgccgcc ttaatcgcaa tatcggcagt ttccaaatca cgaatctcac cgaccataat
    33601 gatgtccggg tcctgacgca ggaaagactt caaagcagcg gcaaaagtca ggccctgctt
    33661 atcattgacg ttaacctgat tgatgcccgg caggttaatc tcggcagggt cttccgccgt
    33721 tgcaatattt accgactccg tattcaaaat attcaaacag gtatagagcg acaccgtctt
    33781 acccgaaccc gtcgggccgg ttaccagcac catcccgtaa ggacggtgaa tcgcctccaa
    33841 caacaatttt ttctgaaacg gctcaaaacc gagctggtcg atgttcaaag acgcggcatc
    33901 ggaattcaaa atccgcatca caaccttttc gccaaacagc gtcggcaatg tgctgacacg
    33961 gaaatcgaca ggcttgccgc ccttttgaaa ggtcagctgc atcctgccgt cctgcggtat
    34021 ccgtttttcg gaaatgtcca aacgcgacat taccttaatc cgggaagcaa gctgccccct
    34081 taccgcaatg ggcggctgaa ccacctcgcg gagctgcccg tccacacgga aacggatacg
    34141 ggcattgtgt tcgtaaaact cgaaatggat gtccgatgcc ccgctgcgca aggcatccga
    34201 caaagtctta tggataaacc tcggaacagg gccgtcttct gcctcctcgt tgtcgatata
    34261 cagggggtgg ctttcctctt cctcctgccc ctccccaagc tcctgaagca gcgatgtcga
    34321 acgcgaaccc acccaatcga gcaaacccgc caactggtca tcctcgacaa tgaccaactc
    34381 aacctcaatc cctgcggcag aaacggtttt ctgaatttgc ggcatctgtg tcggatcgga
    34441 aaccgcaaaa aatactttgt cgccccgacg gaaaaccggc acacagtgga actccaccat
    34501 ctgctcctcc gtcaacaccc ccatcagcac cctgtggcgc ggataatgac gcaaatcaag
    34561 aatcgaataa ctgaacaccc tcgcaatcaa tgccgcaagc gacttgggcg aaatgacacc
    34621 gtctgaaaac agcatcggca acacttcctt acccgcctgc gactccttgt agtaatgctc
    34681 ggcctgctca acagtaacca cctggttttg aaccagaatc ctcagcaaac ctacgctcat
    34741 acgaccttat ccccaaattt attcattgtt atacctgtac ag
//
LOCUS       DAXHMX010000004        33564 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHMX010000004 DAXHMX010000000
VERSION     DAXHMX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.004"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            81..1046
                     /locus_tag="ACFM4S_000139"
     CDS             81..1046
                     /locus_tag="ACFM4S_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6049904.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQTEGASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESSKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGSSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKEQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1064..1240
                     /locus_tag="ACFM4S_000140"
     CDS             1064..1240
                     /locus_tag="ACFM4S_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049905.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFYWNLPYPLPDTEA
                     SNKAFRSDGSDCLV"
     gene            1275..2243
                     /locus_tag="ACFM4S_000141"
     CDS             1275..2243
                     /locus_tag="ACFM4S_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6049906.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2233..3207
                     /locus_tag="ACFM4S_000142"
     CDS             2233..3207
                     /locus_tag="ACFM4S_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6049907.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFLTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPAVGKFGFELVVMMGGSLLLFYTLVKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFMAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3252..3878
                     /locus_tag="ACFM4S_000143"
     CDS             3252..3878
                     /locus_tag="ACFM4S_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC6049908.1"
                     /translation="MTQERFPSFFNQAPTITVRDALAEFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPE
                     MRELMPKAVGGSASAEELKRFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3962..4720
                     /locus_tag="ACFM4S_000144"
     CDS             3962..4720
                     /locus_tag="ACFM4S_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6049909.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLACTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(4779..5117)
                     /locus_tag="ACFM4S_000145"
     CDS             complement(4779..5117)
                     /locus_tag="ACFM4S_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6049910.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5582..9568
                     /gene="purL"
                     /locus_tag="ACFM4S_000146"
     CDS             5582..9568
                     /gene="purL"
                     /locus_tag="ACFM4S_000146"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6049911.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQAYGKPDHIASPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATGDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPYAT
                     EGNPFGKKPFNAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQ
                     TPVADCAVTMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCIGEAITNIAAVNIGDIG
                     NIKLSANWMAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGADK
                     KSVVSPLSLIISAFAPVEDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYN
                     NMSGDAPDLDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLD
                     IDLNLLLAQTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVA
                     DIINLFYQQQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRL
                     RDNPACADSEFALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQ
                     IEMAAAFTRAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSIL
                     FHPALRDQFAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARL
                     SMVHVPKSASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQV
                     TQTYPLNPNGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLF
                     AGARKALG"
     gene            9663..10421
                     /gene="gloB"
                     /locus_tag="ACFM4S_000147"
     CDS             9663..10421
                     /gene="gloB"
                     /locus_tag="ACFM4S_000147"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6049912.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10735..15212
                     /locus_tag="ACFM4S_000148"
                     /pseudo
     CDS             10735..15212
                     /locus_tag="ACFM4S_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
     gene            15444..16898
                     /gene="mgtE"
                     /locus_tag="ACFM4S_000149"
     CDS             15444..16898
                     /gene="mgtE"
                     /locus_tag="ACFM4S_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6049913.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(16984..17892)
                     /gene="hslO"
                     /locus_tag="ACFM4S_000150"
     CDS             complement(16984..17892)
                     /gene="hslO"
                     /locus_tag="ACFM4S_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6049914.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            18137..18892
                     /locus_tag="ACFM4S_000151"
     CDS             18137..18892
                     /locus_tag="ACFM4S_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6049915.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            18911..19129
                     /locus_tag="ACFM4S_000152"
     CDS             18911..19129
                     /locus_tag="ACFM4S_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049916.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            19274..19618
                     /locus_tag="ACFM4S_000153"
     CDS             19274..19618
                     /locus_tag="ACFM4S_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6049917.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            19618..20310
                     /locus_tag="ACFM4S_000154"
     CDS             19618..20310
                     /locus_tag="ACFM4S_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6049918.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            20377..21597
                     /gene="argJ"
                     /locus_tag="ACFM4S_000155"
     CDS             20377..21597
                     /gene="argJ"
                     /locus_tag="ACFM4S_000155"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6049919.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            21657..23066
                     /locus_tag="ACFM4S_000156"
     CDS             21657..23066
                     /locus_tag="ACFM4S_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6049920.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            23256..24647
                     /gene="hrpA"
                     /locus_tag="ACFM4S_000157"
     CDS             23256..24647
                     /gene="hrpA"
                     /locus_tag="ACFM4S_000157"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6049921.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            24748..25875
                     /locus_tag="ACFM4S_000158"
     CDS             24748..25875
                     /locus_tag="ACFM4S_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC6049922.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            26052..27629
                     /gene="lpt3"
                     /locus_tag="ACFM4S_000159"
     CDS             26052..27629
                     /gene="lpt3"
                     /locus_tag="ACFM4S_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC6049923.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            27731..30937
                     /locus_tag="ACFM4S_000160"
     CDS             27731..30937
                     /locus_tag="ACFM4S_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6049924.1"
                     /translation="MDARSNPANVSDGLQNSSSHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAASKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRSPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTSTRGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAAIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVIASERVTLYGLTVLPRRPVSYGRIAPEEAR
                     EIFIRGALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPEREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSASASTFSDDLRPANLQQTAPSPVGEGWGESKTVATQTNFSATSTLSDDPKPKKQPA
                     PQKGRLKPLPLADIRTFQAWLKTAECDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQT
                     ADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMLREKIQLLIKALPK
                     QIRRICVPVPDFITKFLESNPDRQATIIPQLAHFIAKSASDMRILEQIDQDAWAAQEL
                     PEHCYLNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTTWDIGT
                     LPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQ
                     VKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKR
                     ARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPGFATRTPWA
                     QWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKNDGLVKQGQPISDNL
                     AAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEKIF"
     gene            31004..31834
                     /locus_tag="ACFM4S_000161"
     CDS             31004..31834
                     /locus_tag="ACFM4S_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6049925.1"
                     /translation="MSLENFQLIKTQLGVPSYENDRVAIYQGDCIQLMDKISKGSFQM
                     TITSPPYNIGKEYESILDLEHYLEWCERWMQSIHHLTEDSGCFWLNLGYLHVEKKGLA
                     VPIPYLLWDKTNFFLLQEVVWNYAAGVACRNRFSPRNEKLLWYVKNPAKYTFNLDAVR
                     DPNVKYPNQKKNGKLKCNPLGKNPTDVWQIAKVTSGRNRSSRERTAHPAQFPLELIER
                     IIRASSNQNDVILDPFIGSGTTALAGLKNNRFVIGFEINDEYIDIIKERFKEYDLQNT
                     "
     gene            complement(31891..32490)
                     /locus_tag="ACFM4S_000162"
     CDS             complement(31891..32490)
                     /locus_tag="ACFM4S_000162"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScaI family restriction endonuclease"
                     /protein_id="HFC6049926.1"
                     /translation="MNRSKISPYASLNEELWISKTEELIKQHPLSEKEIVQLCLDSWE
                     NIFSSRIGNLQIGKNFFPSPQIVGALLHALIPANIEARFENWQGEKNKYDKDIVYIKD
                     DYYSIELKTSSDPKYIFGNRSYAQPQDGITGKSKNGYYLTINFEKFMPDLKLRPEIKI
                     IRFGWLDHTDWIAQTAATGQQARLSPEAYRSKLKILYQK"
     gene            complement(32492..32707)
                     /locus_tag="ACFM4S_000163"
     CDS             complement(32492..32707)
                     /locus_tag="ACFM4S_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6049927.1"
                     /translation="MENELVEFGKRVKHYRLLNGWSQEELAEKVGLHRTYIGSVERGE
                     RNISLLNISLLAKSFSINISELVEGIK"
     gene            complement(33139..33564)
                     /locus_tag="ACFM4S_000164"
                     /pseudo
     CDS             complement(33139..33564)
                     /locus_tag="ACFM4S_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8371 a        10016 c         8135 g         7042 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacacct atgttacgtt tgactgcttt agccgtatgc accgccctcg
      121 ctttgggcgc gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcgg
      181 tttccgccgc acaaaccgaa ggcgcgtccg ttaccgtcaa aaccgcgcgc ggcgacgttc
      241 aaataccgca aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacaccttga
      301 gcaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa ccgcctgccg tatttagagg
      361 aatatttcaa aacgacaaaa cctgccggca cgctgttcga gccggattac gaaacgctca
      421 atgcctacaa gccgcagctc atcatcatcg gcagccgcgc agccaaagcg tttgacaaat
      481 tgaacgaaat cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagct
      541 caaaagagcg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc
      601 tgaaggcgga aatcgacgcg tcttttgaag ccgcgaaaac tgccgcgcaa ggcaaaggca
      661 aaggtttggt gattttggtc aatggcggca agatgtccgc cttcggctcg tcttcacgac
      721 tgggcggctg gctgcacaaa gacatcggcg ttcccgctgt tgacgaagcc atcaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 tcgtccttga ccgcagcgcg gccatcggcg aagagggtca ggcggcgaaa gacgtgttgg
      901 ataatccgct ggttgccgaa acaaccgctt ggaaaaaaga acaggtggtt tacctcgttc
      961 ccgaaactta tttggcagcc ggtggcgcgc aagagctgct gaatgcaagc aaacaggttg
     1021 cggatgcgtt caacgcggca aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcttcctgtg tttcagacgg cattgcccga tacgggcgtt atcgcgggaa
     1141 tggcggcaag cgtttttatt ggaacttgcc ctatcctctg cccgatacgg aggcttcaaa
     1201 caaggctttc cgctccgacg gttcggactg ccttgtttga atcttctacg ccttaacgct
     1261 tttcctttct gtttatgact gccaaacctt tttccctcaa cctgaccaac ctcctgctgc
     1321 tggcggtatt gtttgccgtc agcctgtcgg tcggcgttgc cgatttccgc tggtcggatg
     1381 tgttttcgct gtccgacagc cagcaggtta tgttcatcag ccgcctgccg cgcacgtttg
     1441 cgattgtgct gacgggcgcg tcgatggcgg tggcggggat gattatgcag attctgatgc
     1501 gtaaccgttt tgtcgagcct tctatggcgg gcgcgggtca gagtgcggct ttgggtttgc
     1561 ttctgatgtc cctgctgctg cctgccgcgc cgctgccggt caaaatgtcg gttgccgccg
     1621 ttgccgcgtt aatcgggatg ttggtgttta tgatgcttat ccgccgcctg ccgccgacgg
     1681 cgcaactgat ggtgcctttg gtcgggatta ttttcggcgg cgtggttgag gcggtggcca
     1741 cctttattgc gtatgaaaac gaaatgctgc aaatgctggg cgtgtggcaa cagggcgatt
     1801 tttcgagcgt gctgctgggg cggtacgagc tgctttggat tacgggcggt ttggcggtgt
     1861 ttgcctatct gattgccgac cggctgacga ttttggggct gggcgaaacg gtcagcgtga
     1921 atttgggttt gaaccggacg gcggtgttgt ggtcggggct gattattgtg gctttgatta
     1981 cgtcgctggt tatcgttacg gtcggcaata ttccgtttgt cgggctggtc gtgccgaaca
     2041 tcatcagccg cctgataggc gacaggctgc gccaaagcct gcctgcggtg gccttgttgg
     2101 gcgcgtcttt ggttttattg tgcgacatta tcggacgcgt gattgtgttt ccgtttgaaa
     2161 ttccggtatc gaccgtcttc ggcgtattgg gtacggcgtt gtttttatgg cttttgttaa
     2221 ggaaacctgc tcatgccgtc tgaaaaaaat atcggtttta tggcaggaag cagccgtccg
     2281 ttgtgggttg cctttgcgct gttgctggtt tcctgcgtcc tgtttttaac gctcaacgtc
     2341 aaaggcgatt gggactttgt actgcacctg cgtctgacca aacttgccgc gctgctgatg
     2401 gtcgcctatg cggtcggcgt gtccacgcaa ctcttccaaa cgctgaccaa caatccgatt
     2461 ctgacccctt cgattttggg tttcgattcg ctgtatgtgt ttttgcagac cttgctggtg
     2521 tttacgttcg gcggcgtggg ctatacgtcc ctgcctgcgg tcggcaagtt cggctttgaa
     2581 ttggtcgtta tgatgggcgg ctcgctgctg ttgttctaca cgctcgtcaa acaaggcggg
     2641 cgcgatttgt cgcgcatgat tttaatcggc gtgattttcg ggattttgtt ccgcagcctg
     2701 tcgtcgctgc tttcgcgcat gatcgacccc gaagaattta tggcggcgca ggcgaatatg
     2761 tttgccggat tcaataccgt ccacagcgag cttttgggca taggcgcgct gattctgctc
     2821 gtcagcgcgg cggtcgtttg gcgcgaacgc taccgcttgg acgtacacct tttggggcgc
     2881 gaccaagccg tcaatttggg catcagctac acgcgcaaca ccttatggat actgctttgg
     2941 attgccgcat tggtggcgac ggcgaccgcc gttgtcggcc cggtaagctt tttcgggctt
     3001 ctcgccgcct cgcttgccaa ccacttttcc ccgtccgtgc gccattccgt ccgcctgccg
     3061 atgacggttt gtgtcggcgg catcctcctg gtcggcggac aaaccgtatt cgaacacttc
     3121 ttgggcatga aggcggtatt aagcgtggtg gtcgaatttg cgggcggact cgttttcctc
     3181 tatctcgttt taaaacacaa aaaatgacgg atgccgtctg aacggcagcc tggctcgaaa
     3241 ggacaaacca tatgacgcaa gaacgtttcc catcattctt caaccaagcc ccgaccatca
     3301 ccgtcaggga cgcgcttgct gaattcctcg gcgcggccga aaatggcatc ctcacttacc
     3361 gctacgccga tgccgtgcgc ctgtgcggcc actcctgccc gaccgtcgcc ggcgcgtacc
     3421 tgatggttat caaaggtctg aaagcccttt acggcgaaga gctgcccgaa cgcggcggca
     3481 tcgaagccgc catgcaggga gcgcgcgacg aaggcacggt cggcgtaacc gcgtccgtcg
     3541 tccaactcct caccggcgca gccccggaaa ccggcttcgg aggcatcgga atgcagggac
     3601 gcttcgcccg ccgtaacctc ttatcctttg gtgcaggcga aatcaacggc acactgaccc
     3661 tgcgccgcaa agacaacggc aaaaccgtcg ccgtcagcct caacgccgcc ctacaaccct
     3721 ttgcacccga aatgcgcgaa cttatgccca aagccgtcgg cggcagcgca agcgcagaag
     3781 aactcaaacg cttcggacaa ctctggcagg cacgcgttaa agcatttttg accgaatcgg
     3841 cggacgaccc gcagttcgtc atcgtccgcg aagtgtgagc gttcagacgg cattccgaat
     3901 ttcaaatgcc gtctgaaccc ccgccaaaca acaacaaacc tacgcccgac aagcatccgc
     3961 catgattacc atccgcaacg tcagctaccg catcggcaca cgccccatcc tcgacaacgt
     4021 cagcctcgac atccccgaag gcggcattac cgccctcgtc ggccccaacg gtgcgggcaa
     4081 atccaccctg ttttccttta tggcgcggct gcgcccgctt gaaagcggca gcatcgccta
     4141 ccgcggcaaa aaccttgcct gcacccccac cgccgaactc gccaaaaccc tgtccatcct
     4201 cacccaagaa aacagcatca tgagccgcat caccgtgcgc gacctgctca tgttcggccg
     4261 ctatccctac catcaaggca gaccgactgc cgaatgccgc cgtatcgtta acggtgcaat
     4321 cgaagaattc cacctgcaag acttcgccga ccgctacctg accgagcttt ccggcggaca
     4381 acgccaacgc gccatgattg ccatggtgtt ctgccagcgc accgactacg ttttgctgga
     4441 cgaaccgctg aacaacctcg acatgtatca tgcccgctcc ctcatgcaaa tcctgcgccg
     4501 gctgaccgac gaacacaagc gcaccaccgt cgtcgtatta cacgacatca accaggccgc
     4561 cgcctacgcc gaccacgtcg tcgccatgaa aaacggccaa gtcgccatgc agggcaagcc
     4621 cgacgacatt ttcaccgccg caaacatcaa aaccctgttc gatatggatg tgtccgtcct
     4681 cgaccaccaa agcaaaaaac tcatcgtcca ccatatctga agctcaaaaa gaaaatgccg
     4741 tctgaaaacc tttcagacgg catttccatt ccaaacgctc aaaccgccgc gtccgcacgt
     4801 tcgcccgtgc ggatacggat tgcctcctcg accggcagca caaaaatctt gccgtcgccg
     4861 atttttcccg aacgcgccac ctcgacaatc acgtcaatcg cgcgttccac agcatcatcc
     4921 gccaacacca gctcgatttt gactttgggc aggaaatcga cggcgtattc cgcaccgcga
     4981 tagatttccg tatgcccctt ctgcctgccg aaccctttga cctcgctgac ggtcatgccc
     5041 gtaatgccga tttccgtcaa cgcctcgcgc acgtcgtcga gtttgaacgg tttgacaatc
     5101 gcctcgattt ttttcatcac attctccatt aattaacgta aacaaacgac aaatatagca
     5161 gaaaccgccg gcaaacgata aacggtactg ttcgcccggc gatgcggcga tttgccggaa
     5221 accgtcattc ccgcgcaggc gggaatcccg atctctgcat ttcagcaata tttaaaggtt
     5281 tctgtaaatc caaacttcca tattcctaac cgtacggaaa cgacggcatt tgatctaaaa
     5341 acatacggaa gcagttgccc gcccctctac ttcctgcaat tatccgcata cccggctttg
     5401 ggtaaaaatc agaaaaacag ataaaaccct tattccttaa tatcttcaga cggcacctgt
     5461 attttcccca actgtatgcc gtctgaagat tgtcaacatt ttaagccaaa atatccaagc
     5521 aatacaaacc tgtccggcgt ataatccctc tttttcccaa ccgcatttga gagccgaatc
     5581 catgtctgtc gttttgccct tgcgcggcgt taccgccctt tccgatttcc gtgtcgaaaa
     5641 actcttgcaa aaagccgccg cactcggact gcccgaagtc aaattaagca gcgaattttg
     5701 gtatttcgcc ggcagcgaga aagcacttga tgccgcgact gtcgaaaaac tgcaagcctt
     5761 gttggcggcg caaagcgttg aacaaacgcc aaaagcgcgc gagggcttgc atttgttttt
     5821 ggtcacgccc cgtttgggta cgatttcgcc gtgggcttcc aaggcgacca atatcgcgga
     5881 aaactgcggt ttggcaggca tagagcgcat cgagcgcggt atggcggtgt ggctggaagg
     5941 cgcgcttacc gacaggcaaa aacaacaatg ggcggctttg ctgcacgacc gcatgaccga
     6001 atccgtattg cccgattttc agacggcatc caaattattc caccatctcg aatccgaaac
     6061 cttctccacc gtcgatgttt tgggcggcgg taaagaggct ttggtcaaag ccaataccga
     6121 aatgggtttg gcactctccg ccgacgaaat cgattatctg gttgaaaact atcaggcttt
     6181 gcagcgcaat ccgtccgatg tggagctgat gatgttcgcg caggcaaaca gcgaacactg
     6241 ccgccacaaa atcttcaacg ccgatttcat cctcaacggc gaaaaacaac cgaaatccct
     6301 gttcggcatg atacgcgaca cgcacaacgc gcatcccgaa ggtacggtcg tcgcctataa
     6361 agacaattcg tccgtgatcg aaggcgcgaa aatcgagcgt ttctatccga atgcggcgga
     6421 aaaccaaggc taccgtttcc acgaagaaga cacgcatatc atcatgaaag tggaaacgca
     6481 caaccacccg accgccatcg cgccgtttgc gggcgcggcg acgggtgcgg gcggcgaaat
     6541 ccgcgacgaa ggcgcgacgg gcaaaggttc gcgcccgaaa gcaggcttga ccggctttac
     6601 cgtctccaac ctcaacatcc ccgacctcaa acagccttgg gaacaagcct acggcaaacc
     6661 cgaccatatt gcttcccctt tagacatcat gatcgaaggc cccatcggtg gcgcggcgtt
     6721 caacaacgaa ttcggacgcc ccaacctctt gggctacttc cgcacctttg aagaaaaatt
     6781 tgacggtcag gttcgcggct accacaaacc gattatgatt gcgggcggct tgggcagcat
     6841 tcaggcgcag cagacgcata aagacgaaat ccccgaaggc gcgttgctga tccaactggg
     6901 cggcccgggt atgcttatcg gcttgggcgg cggcgcggct tcttcgatgg ataccggtac
     6961 aaacgacgct tctttagact tcaactccgt gcaacgcggc aaccccgaaa tcgaacgccg
     7021 cgcgcaggaa gtcatcgacc gctgctggca gctcggcgac aaaaacccga ttatctccat
     7081 ccacgacgtg ggcgcaggag gtttgtccaa cgccttcccc gaactcgtca acgatgccgg
     7141 acgcggcgcg gtattcaagt tgcgcgaagt gccgttggaa gaacacggtt taacgccttt
     7201 gcaaatctgg tgcaacgaat cgcaagagcg ttatgtgttg tcgattttgg aaaaagattt
     7261 ggatgccttc cgcgccatct gcgaacgcga acgctgcccg tttgccgtag tcggcacggc
     7321 gaccggcgac ggccatttga aagtacgcga cgatttgttc tccaacaacc ctgtcgattt
     7381 gccgttgaac gttttgctcg gcaaaccgcc caaaaccacg cgcaccgaca aaacggttac
     7441 gccgtatgcg accgaaggga atccctttgg gaaaaaaccg tttaatgccg gcgatatcga
     7501 cattaccgaa gccgcctacc gcgttctgcg cctgcctgcc gtagcggcta aaaacttcct
     7561 gattaccatc ggcgaccgca gcgtcggcgg catgacccac cgcgaccaaa tggtcggcaa
     7621 ataccaaacc cccgtagccg actgcgccgt gaccatgatg ggcttcaaca cctatcgcgg
     7681 cgaagcgatg tctatgggcg aaaaaccggc cgtcgccctg tttgacgcgc ctgcttcggg
     7741 cagaatgtgt atcggcgaag ccatcaccaa catcgcagcg gtcaacatcg gcgacatcgg
     7801 caatatcaaa ctttctgcca actggatggc ggcgtgcggc aacgaaggcg aagacgaaaa
     7861 actctaccgc accgtcgaag cggtttccaa agcctgtcag gcattggatt tgagcatccc
     7921 cgtgggcaaa gacagcctgt cgatgaaaac cgtttggcag gatggcgcgg acaagaaatc
     7981 cgtcgtctcg ccattgagcc tgattatctc cgcgttcgcg ccggtggaag acgtacgcaa
     8041 aaccgttacg cccgaactga aaaacgtcga agacagcgta ttgctgttta tcgatttggg
     8101 cttcggcaaa gcgcgtatgg gcggctcggc gtttggtcag gtgtacaaca atatgagcgg
     8161 cgacgcgccc gatttggacg atacaagtcg tctgaaagcg ttttacaacg tgattcagca
     8221 gcttgtcgcc gaagacaaac tcttggcgta tcacgaccgc agcgacggcg gtttgtttgc
     8281 cgttttggta gaaatggcgt ttgcggggcg gtgcggcttg gatatagatt taaatttatt
     8341 gcttgcacaa acatttatta ccaaccatac cgctctgtct caatcattgc ggactgaaga
     8401 ggtaaaagcg ttggctgaat ggcaagaaac cattgcccgc acattattta atgaagagtt
     8461 gggtgctgtt atccaagtta gaaaacaaga tgttgccgat attatcaatt tattctatca
     8521 acaacagctg catcataatg tctttgaaat cggtacgtta actgatgaga acacgttaat
     8581 catccgcgac gggcaaacgc accttatttc tgacaaccta atcaaactgc aacaaacctg
     8641 gcaagaaacc agccatcaaa tccaacgcct gcgcgacaac cctgcctgcg ccgacagcga
     8701 gtttgccctg attggcgaca acgaacgcag cgcattgttt gccgacgtga agttcgacgt
     8761 gaacgaagac atcgccgcgc cgtttatcaa cagcggcgcg aaacccaaaa tcgccatcct
     8821 gcgcgaacag ggcgtaaacg ggcaaatcga aatggccgcc gcgttcactc gcgccggttt
     8881 cgatgcctac gacgtgcata tgtccgacct gatggcaggc cgcttccgcc ttgccgactt
     8941 caaaatgctg gcggcgtgcg gcggcttcag ctacggcgac gtactcggcg cgggcgaagg
     9001 ctgggcgaaa tccatcctgt tccaccctgc cttgcgcgac caatttgccg ccttctttgc
     9061 cgatccggac acgctgacat tgggcgtgtg caacggctgc cagatggtca gcaacctcgc
     9121 cgagattatc ccgggtgcag aggcatggcc taaattcaaa cgcaacctga gcgaacagtt
     9181 tgaagcacgc ctgagcatgg ttcacgttcc gaaatcagcg tcgctgattc tgaacgaaat
     9241 gcaaggctcc agcctgcctg tcgtggtcag ccacggcgaa ggccgcgccg acttcgcgct
     9301 tcacggcggc aatatttccg ccgatttggg catcgcgctg caatatgtgg acggtcaaaa
     9361 ccaggttacc caaacctatc cgctcaaccc caacggctcg ccgcaaggca tcgccggcat
     9421 taccaacgcc gacggccgcg tgaccatcat gatgccccac cccgaacgcg tgtaccgcgc
     9481 cgcgcaaatg agctggaaac cggaaggctg gacggaactg tccggctggt accgcctctt
     9541 tgccggcgca cgtaaagcct tgggctaacc gccctactca aaccaatgcc gtctgaagaa
     9601 tatttcagac ggcgttccgg cataccatcc tttaaacggt atccgtccac cgaggaacac
     9661 tcatgaaaat cacccccgtc aaagccctaa ccgacaacta catctggatg atacagcacg
     9721 gcaaccatgc cgtctgcgtc gacccttccg aaccctcgcc cgtcttagaa ttcctcgtcc
     9781 gcaaccgcct catgcttgcc caaacatggg taactcaccc ccaccccgac cacgagggcg
     9841 gtgcggcggc actctggcgc ggctacatgg aatcgcccgt ttacggcgaa tccgacatcg
     9901 aagcagcaac ccacaccgta accgccggca cccaattcac cttcggcgac ggacaggtta
     9961 ccgtttgggc aacacccggc cacacagacc gccacaccag ctaccttctc gaaacttcag
    10021 acggcataca cgtcttttgc ggcgacaccc ttttttccgc cggctgcgga cgcgtgttta
    10081 ccggcacaat cgaacagctt tacgacagct tccaacgctt caaccgcctg cctgaaaaca
    10141 ccctgttcta cccggcacac gaatacaccg ccgccaacct gcgtttcgcc gcccatatcg
    10201 agccggacaa cgccgacatt cagacggcac tgaaagcggc ggcgcatacg cctaccctgc
    10261 ccgttaccct cgcgcacgaa cgccgcgtca atccgttttt gcgcgtcgac ctgccgcacg
    10321 tcagagaccg cgccgaggca ttgagcggga aaacgttaaa cagcagcctc gatacctttg
    10381 tcgcgctgcg tgaacttaaa aaccaatacc ggacgaaata aaacaatggg aaaacgcagc
    10441 cattcctagg atttttatta aaatcttaaa taaaatcata caatcatcgc caatagacga
    10501 aaggacaccg ttgccttata atcaaacaaa aacaaaatat ataatatagt ggattgaatt
    10561 taaatcagga caaggcgacg aagccgcaga tagtacggca aggcgaggca acgctgtact
    10621 ggtttttgtt aatccactat attgttaatc cactatataa atccagcaca aaacgggatc
    10681 ggtgattctt gtccgcaaga atcgttgatt ttctctatta cacggataat catcatgcgc
    10741 ttcacacaca ccaccccatt ttgttccgta ttgtccaccc tcggtctttt tgccgtttcc
    10801 cctgcttact catccattgt ccgcaacgat gtcgattacc aatattttcg cgactttgcc
    10861 gaaaataaag gcgcgttcgc cgtaggtgca agcaatattt ccatccaaga caagcaaggc
    10921 aaaatattag gcagggttct caacggcatc cccatgcccg acttccgagt cagcaaccgg
    10981 caaaccgcca tcgccaccct gattcacccc caatacgtca acagtgtcaa acacaacgtc
    11041 ggctacggtt ccatacaatt cggcaacgac acccaaaatc cagaagaaca agcctatacc
    11101 taccgcctcg tatcacgcaa cccgcacccg gactacgact accaccttcc ccgcctcaac
    11161 aaactggtta ccgaaatctc acctgccgca ctcagcagcg tacccttgct tggaaacggc
    11221 cagccaaagg ccaatgccta cctcgatacc gaccgcttcc catactttgt acgactcggc
    11281 tcaggcacgc aacaagtccg caaagcagac ggcacgcgta cacgaaccgc cccggcatac
    11341 caatacctga ccggcggcac gccgctgaaa gtattggggt tccaaaacca cggcttactc
    11401 gtcggcggca gcctgaccga ccaacccctt aacacctacg caatcgccgg agacagcggt
    11461 tcccccctgt ttgccttcga caagcatgaa aaccgctggg tgcttgcggg cgtactcagc
    11521 acctacgccg gcttcgataa cttcttcaac aaatacatcg tcacgcaacc cgaattcatc
    11581 cgttccacca tccgccaata cgaaacccgg ctggatgtcg ggctgaccac caacgaactc
    11641 atatggcgcg acaacggtaa tggcgacagc accctgcaag ggctcaacga acgcatcacc
    11701 ctgcccattg caaacccttc gcttgcccca caaaacgaca gcaggcacat gccgtctgaa
    11761 gatgccggca aaacgctcat cctatccagc aggttcgaca acaaaacact gatgctggca
    11821 gacaatatca accaaggcgc aggcgcattg cagttcgaca gcaacttcac cgtcgtcggt
    11881 aaaaaccaca catggcaagg tgcaggcgtt atcgtagccg acggcaaacg cgtcttctgg
    11941 caagtcagca accccaaagg cgaccggctc tccaaactgg gcgcaggcac gcttatcgcc
    12001 aacggacaag gcatcaacca gggcgacatc agcatcgggg aaggcactgt cgtactcgcc
    12061 caaaaagctg cttcagacgg cagcaaacaa gcattcaacc aagtcggcat caccagcggc
    12121 aggggcacgg ccgtcctcgc cgacagccag caaatcaaac ccgaaaacct ctatttcggc
    12181 ttcaggggcg gacggctcga cctcaacggc aacaaccttg cctttaccca tatccgccat
    12241 gcggacggcg gcgcgcaaat cgtcaatcac aaccctgacc aagccgcgac actgacgctg
    12301 accggcaacc ccgtcctcag tcccgagcat gtcgagtggg tgcaatgggg caaccgtccg
    12361 caaggcaacg cggcggttta cgaatacatc aacccgcacc gcaaccgtcg gaccgactac
    12421 ttcatactca aacccggcgg caacccgcgc gaatttttcc cgttaaatat ggaaaactca
    12481 acaagctggc aatttatcgg caacaacagg caacaggccg ccaaacaagt cgcccaagcc
    12541 gaaaatgccc gccccgacct aattaccttc ggcggatact tgggtgagaa cgcgcaaacg
    12601 ggcaaagccg caccgagtta cagcaaaacc aatatcgaat gggtgaatgg tccaaacacc
    12661 ccacacaagc cctcgcccta tacgaatacg ccaacccgca caggggcagg cgtaccgacg
    12721 attaccgcct caaagaaggc ggcaaccccg gcgcctactt cccatggaat gcctacagca
    12781 cccaagactg ggaatttctc ggacatgacc ggcgaagcag ccatagaaaa aacccgccat
    12841 atcgcaaatg ccgccgtata cggccggccc gaataccgtt acaacggcgc actcaacctg
    12901 cactatcgtc ccaaacgcac cgacagcacg ctgttgctca acggcggcat gaaccttaac
    12961 ggggaagtct tgattgaggg cggcaatatg attgtgtcag gcaggcccgt accccatgcc
    13021 tacgaccacc aggccaaacg cgaacccgtt cttgaaaacg aatggaccga cggcagcttc
    13081 aaggctgcac ggttcaccct gcgaaaccat gcccgactga cggcagggcg caataccgcg
    13141 catctggacg gcgacataac cgcatacgat ctgtccggca tcgacctcgg ctttatccaa
    13201 ggcaaaacac cggaatgcta ccgctcctac catagcggca gcacccactg cacacccaac
    13261 gccgttttaa aagccgaaaa ctatcgtgca ctacctgcaa cgcaagtacg cggcgacatt
    13321 acccttaacg accgttcaga gctccgcctg ggcaaagcac acctgtacgg cagcatccgt
    13381 gccggcaaag acaccgcagt ccgcatggaa gcagacagca actggacact ttcccagtcc
    13441 agccacaccg gcgcactgac gcttgacggc gcacaaatta ccctgaaccc cgatttcgcc
    13501 aataatacac acaacaaccg cttcaacaca ctgaccgtca acggcacact tgacgggttc
    13561 ggcacattcc gattcctgac cggcatcgtc cgaaaacaaa atgccccccc cccctcaaac
    13621 tggaagggga cagccgcggc gcattccaaa tccacgtcaa aaacaccgga caagaacctc
    13681 aaacaaccga atcgcttgca cttgtgagcc tcaatccgaa acacagccac caagcccgat
    13741 tcaccctcca aaacggctat gccgatttgg gtgcctaccg ctacatcctc cgcaaaaaca
    13801 acaacggata cagcctgtac aacccgctca aagaggccga acttcaaatt gaagccacgc
    13861 gtgcggaaca tgagcgcaac caacaggcat acaaccaatt acaggcaacc gacatcagca
    13921 gacaggttca acatgactct gacgcgacca ggcaggcact acaggcctgg cagaacagtc
    13981 aaaccgaact tgcccgcatc gacagccaag tccaatatct gtccgcccaa ttgaaacaga
    14041 cagacccgct gaccggcatt ctgacgcgtg cccaaaacct gtgtgccgca caaggataca
    14101 gtgtcgatat ctgccgtcag gttgccaaag ccgccgacac gaacgacctg acactcttcg
    14161 aaaccgaact ggatacgtat atagaacgtg tagaaatggc cgaatccgaa cttgacaaag
    14221 cacggcaagg cggcgatgcg caagccgtcg aaacagcccg gcacgcctac ctgaacgcac
    14281 tcaaccgtct gtcccgacaa atccacagtt tgaaaaccgg cgttgccggc atccgtatgc
    14341 cgaacctggc cgaactgatc agccggtcgg ccaacaccgc cgtttccgaa caggccgcct
    14401 acaataccgg ccggcaacag gcgggacgcc gcatcgaccg ccaccttacc gatccgcagc
    14461 agcaaaacat ctggctggaa accggtacgc aacaaaccga ctaccatagc ggcacacacc
    14521 gtccctacca acaaactacc aactatgcac atatcagcat ccaaaccggc atcaccgacc
    14581 gtctcagtgt cggtacaatt ttaaccgatg agcgcacaaa caaccgtttt gatgaaggcg
    14641 tatccgcccg aaaccgcagc aacggcgcac atctgttcgt caaaggggaa aacggcgcac
    14701 tctttgccgc ggcagattta ggctacagca acagccgtac ccgatttacc gattatgacg
    14761 gggctgccgt ccgccgccac gcatgggatg caggcatcaa caccggcatc aaaatcgata
    14821 ccggcatcaa cctcagaccc tatgccggca tccgtataaa ccgcagcaac ggcaaccggt
    14881 acgtactcga cggcgcagag ataaacagcc cggcgcaaat ccaaaccaca tggcatgccg
    14941 gcatccgtct cgataaaacc gtcgaactgg gtcaagccaa gctgaccccc gccttcagca
    15001 gcgattacta cgatacccgc caaaacagcg gttccgccct cagcgtcaac gaccgtacct
    15061 tactgcagca agccgcccac ggcacactgc ataccctgca aatcgacgcc ggatacaaag
    15121 gctggaacgc caaacttcat gccgcttacg gcaaagacag caacaccgcc cgccacaaac
    15181 aagcaggaat caaaataggc tacaactggt aacaagccga taaaaatgcc gtctggaacc
    15241 cgcgtttcag acggcatttg cgttaaaaat agtaaaccgt tccaaaaggg agtagaatag
    15301 tgccgtttcc aaccctgcgc ctgtaccgtc aggcttttat tatggacctt cccagttcgt
    15361 ttttactgaa cgccccttcc gattccaaac gacaataacc atcccgccgg aatgcctccc
    15421 cgcacacggc gggcggagca tttatgagca tcgaaccaac ccctccgaac cttgaaaacg
    15481 acggtatcga aaacgatgta gaacgcgttt ccgccgattt cgaccgtgtc cactccctct
    15541 gcgaaattct cgaacctgct tttgaacaaa tcgaaaacgg cacaccgctt gaagacgcgc
    15601 cgctgcgcga caagctgacc gagctgaccg ttctcttgag cgagctgcac cctgccgacg
    15661 tggcggcggt attggaatcg ctaccgccgc gcgaacgcaa tatcgtctgg attctggtca
    15721 aaccggaaga cgacggcgaa gtattgctgg aagtatccga cgcggtgcgc gaaacgctga
    15781 tcgagtcgat ggacaaagac gaattgttgg cagcggtcga tgatttggac gcggacgaat
    15841 tggcggaact ggcagacgat ttgccgcacc aagtggttta cgaagcgcta caaacccgcg
    15901 atgaggaaga acgcgcccaa gtcaaagcgg caatgtccta cgaagacaac caagtcggtg
    15961 cgattatgga cttcgagttg gtcagcatcc gcgccgatgt cgcctgtgaa gtggtgctgc
    16021 gctatctgcg ccgcttcgac agcctgcccg accataccga caagattttt gtggtcgatg
    16081 aaaacgacgt actgcaaggc gtgctgccca tccgcaaact tttggtcgcc gatcccgaag
    16141 atttggtgga aaacgtgatg gcgaaagatg tcgtgcgttt ccgcgccgaa gacgacgtgg
    16201 aagaagcggc gcaggcgttt gaacgctacg acttggtaac tgcgccggtt gtcgatgaaa
    16261 acaaaaagct catcggtagg attaccgttg acgagatggt ggacgtcatc cgtgaagaat
    16321 ccgaagcgga tatgttgaac atggcaggtt tgcaggaaga ggaagacctg ttcgcccccg
    16381 tcttggattc cgtcaaaaac cgctggatgt ggctcgccgt caacctctgc accgccttcc
    16441 tcgccagccg cgtcatcggc gcgtttgaag gcagtatcga aaaaatcgtt gcactcgccg
    16501 cgctgatgcc catcgtcgcc ggtatcggcg gcaactcggg caaccagacg attaccatga
    16561 ttgtccgcgc catggcgatg gggcagttga cgggaatgca ggcggggcgc ttgttgaaaa
    16621 aagaagtcgg cgttgcccta gtcaacggca ttatctgggg aaccgtcatg ggcgcggttt
    16681 catggctgct ctacggcagc ctcggcatcg ggctggttat gattgccgcg atgacgctca
    16741 acctcctgct ggcggcaacc gtcggcgtat taattcccgt ggtaatggaa aagttcggac
    16801 gcgatcccgc actgggcagc tcggtgctga ttaccgccgt taccgactcc ggcggcttcc
    16861 tgattttctt ggggctcgcc accctattcc tgctttaaat gccgtctgaa cccgcgcaaa
    16921 aatgccgtct gaagcggaag ctgcttcaga cggcatttga ctatttatcc ttgttgcaca
    16981 agattattgg acggtatgcc ggggcagccc tttggcaacg ccgaccacat cctccccgaa
    17041 cagcgcgttg acatcggttt cgtcaaacac atatttgctg tggcagaaat cgcaatcgac
    17101 ttcgatgctg ccttgttccg ccaccacgcc gccgacttct tccccgccca gcatcaacag
    17161 catatcgctg actttgccgc gcgaacaggt gcatgaaaat tcaaacgttt ccggctcgaa
    17221 cacgcgcggc ggcgtttcgt ggaacaggcg gtataaaacg tgttgcgcat ccaattctgc
    17281 cagctcctcc gccgtcagcg tgcgcgccag cgtactgacg tgttcccatg cctcttcatc
    17341 caacacctct tcaggcagac gctgcaccag cagaccgccc gccgcttcat cgcttgcaga
    17401 caggacgatg tgcgtatcaa gctgttcgga acgtttcata tagttcatca acatttgcgc
    17461 gataccgtcg ccttccaaag gcactacgcc ctgccagggt tcgccgtctt tgggctgcag
    17521 cgtcaatacg aacacgccgt tgccgcccaa aaggtcgccg aggctttcgt catcggctat
    17581 ttctgccgcc tcgtcccacc gcgcggtcgc acggacggta cggtcggaag tcgcttccgc
    17641 aaccagcatt ttcagccgcc cctgcccctg aacctgcaca atcagcgtgc cttcgttttt
    17701 gaggttgccc gacagcagaa caccggcagc caacagctcg cccaaggcgc ggcggatggc
    17761 ggcgggatag tttttctgtt ttacaatgtg ctgccacacg ttttccagac ggacgtgcag
    17821 cccgcgcacg ggcatatcgt cgaagataaa gcgggtacgc acatcggcgc ggttgatggc
    17881 ggtttgattc atgattttct ctgactgatt gttcggatgg cggctatatg gttgcggtcg
    17941 gcgcgaaaac aagacggacg gcggatgcgc ttcccaaatt atcaataaat tatataaaaa
    18001 tcaacatatt aactcaatct aacaagccgt tttttgccaa acagccgttt ttttatatac
    18061 aatcaacaag atattttcga ctgatacagc ataacatcgc acggcggcac gatgcctcct
    18121 gcgcggaaac accgatatgg attctttttt caaaccggca gtttgggcgg ttttgtggct
    18181 gatgtttgcc gtccgccccg cccttgccga cgagttgacc aacctgctca gcagccgcga
    18241 gcagattctc agacagtttg ccgaagacga acagcccgtt ttacccatca accgagcccc
    18301 cgcccggcgg gcgggcaatg ccgacgaact catcggcagc gcgatggggc ttaacgaaca
    18361 gcccgtttta cccgtcaacc gagtccccgc ccggcgggcg ggcaatgccg acgaactcat
    18421 cggcaacgcg atggggctta acgaacagcc cgttttaccc gtcaaccgag cccccgcccg
    18481 gcgggcgggc aatgccgacg aactcatcgg caacgcgatg ggacttttgg gtattgccta
    18541 ccgctacggc ggcacatcgg tttctaccgg ttttgactgc agcggcttca tgcagcacat
    18601 cttcaaacgc gccatgggca tcaacctgcc gcgcacgtcg gcagaacagg cacggatggg
    18661 tacgccggtt gcccgaagcg aattgcagcc cggagatatg gtgtttttcc gcacgctcgg
    18721 cggcagccgc atttcccatg tcggacttta tatcggcaac aaccgcttca tccacgcgcc
    18781 gcgcacgggg aaaaatatcg aaatcaccag cctgagccac aaatattgga gcggcaaata
    18841 cgcgttcgcc cgccgggtca agaaaaacga cccgtcccgc tttctgaact gatttcccaa
    18901 ggaatacgca atgagtatgc ccgaaatacc caaatggtac gacgatgacg gacagatcgt
    18961 gtcctgtacc gaaaagatca aagtgatgtc cgaaaatatg gccgagctgt atcagacggc
    19021 acaagacgcg tttgaagacg cgctgctgat gggttgcggc gaacgtcagt tgcgcgatta
    19081 cctgctcgcg ctgattgaag gtttggaaaa tccctaccgc aaagtctgaa cacgccccgg
    19141 ttgctgcggc acggtttatc cgtgccgttt ttgcgtttgt gcgcggcttc ggcttttcag
    19201 acggcatatt tgacgttatg attaaacagt taacaagatt tatcacaacg ccgtcaaaag
    19261 acagacacac aacatgaaca tcatccgcgc gctcctcatc atcctcggct gcctcgccgc
    19321 cggcgaaacc gccgttttcc tagcaggcat caaactgccc ggcagcatcg tcggcatggg
    19381 cgtgctgttt gcgcttttgc aggcgggttg ggtcaaaacg tcttggctgc aacagcttac
    19441 cgacgcgctg atggcgaatc tgacgctgtt tctcgtgccg ccctgcgtgg cggtcatcag
    19501 ctatttggat ttgattgccg acgattggtt ttcgatactg gtttccgcct ccgccagcac
    19561 tttgtgcgta ctgctggtta caggcaaggt tcaccgctgg atacggagca ttatctgatg
    19621 aacgaaatcc tcaggcagcc cagcgttctg cttttcctca cgcttgccgt gtacgcgctt
    19681 gcgattatcg tgcgcacgcg cacgggcaat atcttctgca accccgtact cgtcagcact
    19741 atcgtgctga ttgcctacct gaaaatcctc gacatcgatt atgcggtgta ccacaacgcc
    19801 gcgcagttta tcgatttctg gctcaagccc gccgtcgtcg tgcttgccgt gccgctctac
    19861 caaaaccgcc gtaaaatctt caaccaatgg ctgcccgtca tcgtttcaca gcttgcgggc
    19921 agcgttacgg gcattgttac ggggatgtat tttgccaaat ggctgggcgc ggaacgcgaa
    19981 gtcgtcctct cgctcgcgtc caaatctgtt accaacccca tcgccattga aattacccgc
    20041 tccatcggcg gcattcccgc cattaccgcc gccaccgtca tcattgccgg tctggtcgga
    20101 cagattgccg gttacaaaat gctgaagaac acagtcgtca tgccctcgtc cgtgggtatg
    20161 tcgctcggca cggcttcgca cgcgatgggc attgccgcct cgctcgaacg cagccgccgc
    20221 atggcggcat acgcggggct ggggctgacg ttcaacggcg tactgaccgc gctgattgcg
    20281 ccgctgctta tccccgtttt ggggttctga acccgtttca gacggcattt cagcccatgc
    20341 tgtctgaacg ccgacacact cgcaaggaga accgttatgg ctgtcaacct gactgaaaaa
    20401 accgccgaac aactgcccga catcgacggc attgccctct acaccgccca agcaggcgtg
    20461 aagaagcccg ggcataccga cctgacactg attgccgtag ccgccggcag caccgtcggt
    20521 gcagtcttca cgaccaaccg tttctgtgcc gcgcccgtcc acatcgccaa atcgcacctt
    20581 ttcgacgaag acggcgtgcg cgccctcatc atcaacacgg gcaacgccaa cgcgggtacg
    20641 ggcgcacagg gcagaatcga tgctttggca gtgtgtgccg ccgccgcccg gcaaatcggc
    20701 tgcaaaccga accaagtgct gcccttctcc accggcgtga ttctcgaacc gctgcccgca
    20761 gacaaaatca tcgccgcgct gcccaaaatg cagcctgcct tctggaacga agcggcacgc
    20821 gccatcatga ccaccgacac cgtgcccaaa gccgcctcgc gcgaaggcaa ggtcggcgac
    20881 aaacacaccg tccgcgccac gggcatcgcc aaaggctcgg gcatgattca tcccaatatg
    20941 gcgaccatgc tcagtttcat cgccaccgat gccaaagttt cccaacccgt cctccaactg
    21001 atgacgcagg aaatcgccga cgaaaccttc aacaccatca ccgttgacgg cgacaccagc
    21061 accaacgaca gcttcgtcat catcgccacc ggcaaaaaca gccaaagcga aatcgacaac
    21121 atcgccgacc cgcgttacgc ccaactcaaa gaattgttgt gcagcctcgc gctcgaactc
    21181 gcccaagcca tcgtccgcga cggcgaaggt gcgaccaagt tcatcaccgt ccgcgtcgaa
    21241 aacgccaaaa cccgcgacga agcccgccaa gccgcctacg ccgtggcacg ttcgccgctg
    21301 gtcaaaaccg cctttttcgc ctccgacccc aacctcggca ggctgctcgc cgccatcggt
    21361 tatgccggcg ttgccgacct cgataccgac ctcgtagaaa tgtatctcga cgatattttg
    21421 gttgccgaac acggtggacg cgccgcaagc tacaccgaag cacaagggca ggcggtgatg
    21481 tcgaaggccg aaatcaccgt ccgcatcaag ctgcatcgcg gacaagccgc cgccaccgtc
    21541 tatacctgcg acctgtcgca cggatacgtt tccatcaacg ccgactaccg ttcctgaccc
    21601 gacacggctt cagacggcat acataaaatg ccgtctgaac cgccggacaa cataccatga
    21661 cctccacatt cccccgccgc ctcgcccgca aaatccgcca aacccgccgc ctgtcgcgca
    21721 aaagcatcgc ctttctgttc cttttggcag gttcggcact cgtcgccctg accgcgctgt
    21781 tttttgccca tcttgccgat tttgcgctgg aactgaacgc caaactggtt caacaatacc
    21841 cgtggttcgc gtgggtcgcg cttcctttgg gtttgccgct tattgcgtgg ctcacacgca
    21901 aattcgcccc cttcaccgcc ggcagcggca tcccgcaggt catcgcctca ctgtcgctgc
    21961 cctacggcgc acagaaaacg cggctgatcc gcctcgggca gacgctgctg aagattccgc
    22021 tgaccttttt gggtatgctg ttcggcgcgt ccatcggacg cgaaggtccg tccgtgcagg
    22081 tcggcgcggc agtgatgggc gcgtggggcg cgtggtgcaa gaaacacggc ttggcattca
    22141 aagggatgca ggaaaacgat ttgatggcgg cgggcgcggc gggcggtttg gcagccgcgt
    22201 tcaacgcgcc gctggcgggc gtgattttcg ccattgagga actcgggcgc ggcatcatgt
    22261 tgcgctggga gaggcaaatt cttttgggcg tgctcgcctc cggtttcata caggtcgcca
    22321 ttcagggcaa caacccgtat ttttccggct tcaacggcgg cgtattggaa catatctttc
    22381 tgtgggtcgc actgtccggc ctggtttgcg gcgcggcggg cgggctgttc ggacgtttgc
    22441 tctatcgcgg tgcggcggca tttgcaccac gcaagatacg cggcttcatc cgcaaccgtc
    22501 cgctgctgct ggcggcactg atggggctgc tgctcgccct gctcggcacg ttctaccaag
    22561 gcaaaaccta cggcaccggc taccacgaag ccgcccaagc cctgcacggc atctatgaag
    22621 cccccttcgg actcgccgcc gccaaatggc tcgccaccgt attcagctat tgggcaggcg
    22681 ttccgggcgg cattttcact ccctcgctga ccataggcgc ggttttgggc gagcatatcg
    22741 ccgccatcgc cgacatatcg cagggtgcaa acatcatcgt cctcatctgc atggcggcat
    22801 ttctggcggg cgcgacacaa tccccgatta cttccgccgt cgtcgtcatg gaaatgacgg
    22861 gcggacaaag cctgctgttt tggatgctaa ttgcctgcat tttcgcctcg caggtttcgc
    22921 gccagttttc gccgcgtccg ttctaccacg catcgggaat gcgcttccgc cagcgcgtgc
    22981 ttcaggaaac caccgcccaa accggcaatg cgcccgcaag accgcaaaca gcaaacagca
    23041 aaacgggaat gccgtctgaa aattaaaacg cccccgatca aacgccggca gccgccttga
    23101 tttgaatacc gttccgccgc cgcttgaaat ttcagcaaca atgccgtctg aacgacagaa
    23161 tgcggttttc agacggcatt tccacatccc gatattgcct aaacaaaacc gaagcgtttg
    23221 ctataattct attttttacc gcacacgcac caatcatgtt tcccgatttc tcccaaaccc
    23281 tctccaaaga ccgccacttc ctacgttccg ccttcaaaaa tcccaacaaa tacggcgggt
    23341 tgtccaaagt cgaagaaaaa taccgaaaat cgcacgaaat ctttttgaaa cgtttggcag
    23401 ccttgcccaa acccgaattc gacaacaccc tgcccgttca cgaaaaactt gacgaaatca
    23461 aaaaaaccgt tgccgaacat caggtaacga ttatttgcgg cgaaaccggt tcgggcaaga
    23521 ccactcagtt gcccaagatt tgtctggaac tcgggcgcgg ggcggcgggt ttgatcgggc
    23581 atacccagcc gcgccgtttg gcggcgcgtt ccgtagcaga gcggattgcc gaagagctga
    23641 aatccgaaat cggcagcgcg gtcggctata aagtgcgctt caccgaccac acctcgcgcg
    23701 atgcctgcgt caagctgatg accgacggca tcctgctggc ggaaacccag accgaccgtt
    23761 atctcgccgc ctacgacacg attatcatcg acgaagcgca cgagcgcagc ctgaacatcg
    23821 acttcctttt gggctatttg aaacaactcc tgccgcgccg ccccgatttg aaagtcatca
    23881 tcacctcggc aacgatagac gcagaacgct tttcccaaca cttcaacggc gcgcccgtgc
    23941 tggaagtgag cggacgcacc tatcccgtcg aaatcctcta ccgaccgctg atcggcaaag
    24001 acgaagacga cgcggaaata gaattgaccg acgcgattgt cgatgcggcg gacgaattgg
    24061 cgcgatatgg cgaaggcgat attttggtat tcctgccggg cgaacgcgaa atccgcgaag
    24121 ccgccgaagc cctgcgcaaa tccacgctac gccgcaacga cgaaatcctg cccctgttcg
    24181 cacgcctgtc gcacgccgag cagcacaaaa tctttcaccc ctccggagcg aaacgccgca
    24241 tcgtgctggc aaccaacgtc gccgaaacct cgcttaccgt gccgggcatc aaatacgtca
    24301 tcgacaccgg cctcgcgcgc gtcaaacgct attccgcacg ggcgaaagtg gagcagcttc
    24361 atatcgaaaa aatctcccaa gccgccgccc gccaacgctc cggccgctgc ggacgcgtct
    24421 ccgcaggcgt atgtatccga ttgttttcag aagaaaattt caacaaccgc cccgaattta
    24481 ccgaccccga aatcgcccgc agcaacctcg ccgccgtcat cctgcgcatg gcatcgctga
    24541 aactcggcga cgtagcggca ttcccatttt tagaaatgcc cgattcgagg tatattaatg
    24601 atgggtttca ggtgttgctg gaattagggg cagtggaggc cgcctgaaaa caagtaggat
    24661 atacacttct acctgacaaa gaccaacaaa cgataaaact acggcacatc tagatgccca
    24721 acctacaaca ttgcacacgg taaaaacatg aaacacttac tcatcgattt tgaaaacgtc
    24781 cagccgcaaa acttagacaa attactaacc gaaaataccc atatttggct atttataggt
    24841 gtattacaca aaatgttacc tattagtctg gtgcaatccc tactacgttt cggcgaacgt
    24901 gtccatcttg tccagttaca aaaaacgggg aaaaacgcat tggattttta cctgtcctat
    24961 tacctcggac aaattaccgc cacagacccc aatgcccaaa tcggcatact ctcgcgtgat
    25021 ggaggatacg atgttctggt cgaacatatt ttgaaaaacc accaggcgaa gggtatcgtg
    25081 cgcctagcca atatagatga agtacaacat cagaaaatta ctaccgaacc gccgtcagca
    25141 ttgctggaaa acactcctca gcctgaaacc accctcaaac cacagcaacc attaacttcc
    25201 tatttccaag cagccctaac tgcactgcgc cgccccgacg ctttccgccc ctgccgcctg
    25261 cataacctgc gacaaaatct gcgtaagcat attttgagtg atttgtttaa agaaaaaacc
    25321 gatgaagaat gcgaaataac cactgctaac gttatcaata aactcaaagc acaaaacttc
    25381 atcagcattg atgaacagga aaccgtttcc taccatctca gtgataatga tttgttacaa
    25441 agaatccaac gccatatttt aagccaacgt cccaaaacct acgctgattt tcaagccgtc
    25501 gtgcaaaacc gagcagatgc acttcactta acagtcggta ccaacgacat tcaatccttt
    25561 gcgcgacatt tgcgcgacca aaacctgatc cgccaaaaca atgggaaaat tgaatatgca
    25621 ccgtttactg aacctaaacc acagccaacg cccaagcagc ctaaaaaaac cgcatgggaa
    25681 cctgatgaaa ttatttggaa aaaagtgatt gccgcgttat cgttaaagaa ccgtcctaat
    25741 aaaaccaaaa ctttacgcaa tacaatccag gcactcacaa aatccaatgc acaagaaact
    25801 gacaaactgc tacaacattt acaagatacc caagtcctac gtattgacgg tactaaaatt
    25861 gtgtatatca agtaggatgg gcatttatgc ctgacctaca aaaccaaaaa caacctaaca
    25921 cccttaatca actccacaaa cccttttcag acaacctcgt tttttgaaaa acaatctgta
    25981 aacagataac tgctgaagaa taccgttgcc gagccccaaa acctgcctgc aacttttatt
    26041 gtgaactgcc cattatgaaa aaatcccttt tcgttctctt tctctattca tctctactta
    26101 ccgccagcga aatcgcctat cgctttgtat tcggaattga aaccttaccg gctgcaaaaa
    26161 tggcagaaac gtttgcgctg acatttgtga ttgctgcgct gtatctgttt gcgcgttata
    26221 aaacttcgcg gctgctgatt gcgctgtttt tcgcgttcag cattattgcc aacaatgtac
    26281 attatgcggt ttatcaaagt tggatgacgg gtattaacta ttggctgatg ctgaaagagg
    26341 ttaccgaagt cggcagtgcg ggcgcgtcga tgttggataa gttgtggctg cctgcgttgt
    26401 ggggcgtggc ggaagtcgtg ctgttttgta gccttgccaa gttccgccat aagacgcatt
    26461 tttctgccga tatattgttt gccttcctaa tgctgatgat tttcgtgcgt tcgttcgaca
    26521 cgaaacaaga gcacggtatt tcgcccaaac cgacatacag ccgcatcaaa gccaattatt
    26581 tcagcttcgg ttattttgtc ggacgcgtgt tgccgtatca gttgtttgat ttaagcaaga
    26641 ttcctgtgtt caaacagcct gctccaagca gaatcgggca aggcagtatt caaaatatcg
    26701 tcctgattat gggcgaaagc gaaagcgcgg cgcatttgaa attgtttggc tacgggcgcg
    26761 aaacttcgcc gtttttaacc cggctttcgc aagccgattt taagccgatt gtgaaacaaa
    26821 gttattccgc aggctttatg acggcagtat ccctgcccag tttctttaac gtcataccgc
    26881 atgccaacgg cttggaacaa atcagcggcg gcgataccaa tatgttccgc ctcgccaaag
    26941 agcagggcta tgaaacgtat ttttacagcg ctcaggctga aaaccaaatg gcaattttga
    27001 acttaatcgg taagaaatgg atagaccatc tgattcagcc gacgcagctt ggctacggca
    27061 acggcgacaa tatgcccgat gagaagctgc tgccgctgtt cgacaaaatc aatttgcagc
    27121 agggcaggca ttttatcgtg ttgcaccaac gcggttcgca cgccccatac ggcgcattgt
    27181 tgcagcctca agataaagta ttcggcgaag ccgatattgt ggataagtac gacaacacca
    27241 tccacaaaac cgaccaaatg attcaaaccg tattcgagca gctgcaaaag cagcctgacg
    27301 gcaactggct gtttgcctat acctccgatc atggccagta tgttcgccaa gatatctaca
    27361 atcaaggcac ggtgcagccc gacagctatc tcgtgccgct agtgttgtac agcccggata
    27421 aggccgtgca acaggctgcc aaccaggctt ttgcaccttg cgagattgcc ttccatcagc
    27481 agctttcaac gttcctgatt cacacgttgg gctacgatat gccggtttca ggttgtcgcg
    27541 aaggctcggt aacgggcaac ctgattacgg gtgatgcagg cagcttgaac attcgcgacg
    27601 gcaaggcgga atatgtttat ccgcaatgag tggcgtaaaa accaataaag acaaattaag
    27661 atgatgtcgg ggaagatgcc cgaccgacaa gactatgcaa aatatgaaaa accaagtacg
    27721 cggatcaggc atggatgcac gatccaatcc ggccaatgtt tcagacggcc tgcaaaacag
    27781 ttcgagccat atcggtacca acacgcgcta ccgcctgacc aagctcggcg aacaaatggc
    27841 gcgcctgccc atcgacccga aaatcgcgcg cattttgctg gcggcgaaga aacacgactg
    27901 tatggcggaa atattggtga ttgcgtccgc gctgtcgatt caagacccgc gcgagcggcc
    27961 gttggaagcg cgcgatgctg cctccaaggc acacgaacgt tttaccgaca agcagtccga
    28021 tttccttgcc tatctgaaca tttgggacag cttccagcgt gagcgcgaca aaggtttgtc
    28081 caacaagcag ctggtgcagt ggtgccgcca atatttcttg tcgcacctgc ggatgcgcga
    28141 atggcgcgag ctacaccacc agcttgccca aactgcgatt gaaatgggtt tgaccaccaa
    28201 agaagccgct ttcagacgtt ctcccgaagt caggcagctc acgtcgtctg aaaatgcagg
    28261 cgaccaagac ctatccgcca aactcaaaca aaaacaactg gataagaaac aacaccgcgc
    28321 ccaaatccgc gctgccaaag aagcgggcta cgaacaaatc caccgcgccc tgctcaccgg
    28381 cctcatcgcc aacgtcggca tgaaatcacc cgacggcaac gactacacca gcacgcgcgg
    28441 cagccgcttc caccttttcc ccgcctccgc cctgttcaag gcgaagccca aatgggtgat
    28501 ggcggcagaa ctcgttgaaa cgacaaaact ttatgcccgc gacgtagccg ccatccagcc
    28561 cgaatggatc gagcaggaag cgccgcacct cgtccgctac cactatttcg agccgcattg
    28621 ggaacaaaaa cgcggcgaag tcatcgccag cgaacgggta acgctctacg gcctgaccgt
    28681 cctgccgcgc cgccccgtgt cctacggcag aatcgccccc gaagaagcgc gcgaaatctt
    28741 tatccgcggc gcattggtgg cacaggaatg cgatttgaaa gcggactttt ttgtccacaa
    28801 caaaaagctg attaaagaaa ttaccgaact cgaacacaaa tcgcgcaagc aagacgtgct
    28861 ggtcgatgac gaagccctgt ttgcgtttta taacgaacga ctgcccgaaa tggcttggaa
    28921 agacgcgcaa ggcagcgttt ggggaagtga agattccgta cggattattg aatctgacaa
    28981 agccgagagg tcgtctgaaa atgagcgcaa cgagtttcgt aaaaacaagc gtaatgggtc
    29041 tcgccaaaat gaaaatcacg gcaacaccgt aggttgggtt gaaaacccaa catcagccgc
    29101 aactgcaaaa actgttgggt ttgacaaccc aacctacgct actcagcaac ccaccccctc
    29161 ccccgagagg gagggtcggg gagagggcaa aacagttgcc gcacaaacca acttttccgc
    29221 aaccgcagca aaccctctcc cgcaggagag ggaacagagt gcctcagctt caacgttttc
    29281 agacgacctg cgtcctgcaa atctgcagca aaccgccccc tcccccgtgg gggagggctg
    29341 gggagagagc aaaacagttg ccacccaaac caacttttcc gcaacctcaa cactttcaga
    29401 cgaccccaaa cccaaaaagc agcctgcacc tcaaaaaggc cgtctgaaac ccctacccct
    29461 cgccgacatc cgcaccttcc aagcctggct caaaaccgcc gagtgcgaca atccgcgcct
    29521 gctgttcctc agccgcgacg atctgatgca acacgccgcc gcacacatta ccgaagaaca
    29581 gttccccaaa ttctggcaaa ccgcagacgg caaattcaaa ctttcctacc gcttcgagcc
    29641 gcaccatccg ctagacggcg tgaccatgac cgtgccgctg accgtcctca accgcctgca
    29701 cgcgccatcg ctcgaatggc tggtgcccgg tatgttgcgc gaaaaaatcc agctgctgat
    29761 taaagcactg ccgaagcaaa tccgccgcat ttgtgtgccc gtacccgatt tcatcaccaa
    29821 attcctcgaa agcaatcccg accgccaagc gaccatcatt ccccagttgg cgcactttat
    29881 cgccaaaagc gccagcgata tgcggattct cgagcaaatc gaccaagacg catgggcggc
    29941 gcaagaattg cccgaacact gctatctgaa tctgcgcatt atcgacgacg gcggacaaga
    30001 gcttgccggc ggccgcaaac tgcacgaatt gcaacaacag ctcggccaag ccgccgccgt
    30061 caccttccgc gacaacaccc aagaattcga gcgcgacaac gttaccacat gggacatcgg
    30121 aaccctgccc gaatccatca aattcgcccg cggcaaacaa cagctcaccg gctacctcgg
    30181 cctgcaaaaa gaaaaagacg gccgcatcgc cctgcgcctg tttgacacca ccgaagccgc
    30241 cgaacaggca caccgtcaag gcgtcatcga attgatgaag ctgcaattaa aagagcaggt
    30301 aaaagacctg aacaaaggca tccaaggctt cacccaagcc gccatgctgc tcaaacacat
    30361 caacgccgac accctgcgcg acgacctcac ccaagccgtc tgcgaccgcg cctttatcgg
    30421 cgaagacgag ctgccgcgca acgaaaaagc cttcaaagaa caaatcaaac gcgcccgtag
    30481 ccgcctgccc gccgtcaaag aagccctcag ccgctacctg caggaaaccg cagccgccta
    30541 cgccgaactc aacggcaaac tcggcaaaca tccattgacc cacctcctaa gactacgcct
    30601 gcaaaccctg cttgcccccg gcttcgccac ccgcaccccg tgggcacaat ggccgcgcct
    30661 ccccatctac ctcaaagcca tgaccctgcg cctcgaaaaa tacagcagca accccgcccg
    30721 cgacgcagcc cgcgaagccg atatacagga attggaacaa atgtggcagg aaaaaaacga
    30781 cggtttggtg aaacaaggac aacccatttc agacaaccta gccgcgttta aatggatgat
    30841 tgaagaattg agagtgtcac tgttcgcaca ggaattgaag acaccgtatc cagtgtcggt
    30901 gaagaggttg ttaaaagagt gggagaaaat tttttaactg aataattctg ccgatcaaaa
    30961 tttcttgaca aaacaaattt taatgaatac tataagtagc attatgagtt tagaaaattt
    31021 tcaattaata aaaacacaat taggtgttcc cagttatgaa aatgatagag ttgcaatcta
    31081 tcaaggagat tgtatccagt taatggataa gatttcaaaa ggatcattcc aaatgacaat
    31141 cacatctcca ccatacaata ttggtaaaga atatgagtct attttagatt tggaacatta
    31201 tcttgagtgg tgtgaaagat ggatgcaatc aatacatcac ttaactgaag atagcggatg
    31261 tttttggtta aatttaggat acttacatgt tgaaaaaaag ggactagcag ttcctattcc
    31321 ttatctttta tgggataaaa ctaacttttt ccttttacaa gaggtggttt ggaattatgc
    31381 agctggagtt gcatgccgta atcgattttc cccaagaaat gaaaagttat tatggtatgt
    31441 gaaaaatcct gctaaatata cattcaattt agatgctgta cgcgatccca acgttaaata
    31501 tccaaatcag aaaaaaaatg ggaaattaaa atgtaatcct ctaggaaaaa acccaacgga
    31561 tgtttggcaa atagcaaaag tcacttctgg aagaaatagg tcatcgagag aaagaactgc
    31621 tcatcctgcc caatttccat tggaattaat agaaagaata atccgagcaa gtagcaatca
    31681 aaatgatgta atcttagatc catttatcgg ctcaggaaca accgccttag ccggactaaa
    31741 aaacaaccgt tttgtcatag gatttgaaat taacgatgag tatatagata ttataaaaga
    31801 acgcttcaaa gagtatgatc ttcaaaatac ttgatacttg tatataacgg tatagaggat
    31861 agaccctcct ctatactgta tttacaatat ttatttttga taaagtattt ttagtttact
    31921 tctataagcc tctggagaga gcctcgcctg ctggcctgtc gctgcagttt gagcaatcca
    31981 atccgtatga tctaaccaac caaatctgat aattttaatt tctggacgca attttaaatc
    32041 aggcataaat ttttcaaaat ttatagttag ataataacca tttttactct taccagtaat
    32101 tccatcttgt ggttgtgcat aactcctatt accaaatata tattttggat ccgatgaagt
    32161 ttttaattct attgagtaat agtcgtcttt aatatataca atatctttgt catatttatt
    32221 cttttctcct tgccaatttt caaatctagc ctcaatatta gcaggaatta aagcatgtaa
    32281 taacgcccct acaatctgag gagaaggaaa aaaatttttg ccaatttgca aattaccaat
    32341 tctagacgag aaaatatttt cccaactatc taaacaaagt tggacaatct ctttttctga
    32401 caatggatgc tgcttaatca gctcttctgt tttggaaatc cataactcct cattcaaaga
    32461 agcatacggc gatatttttg atctattcat attatttaat cccttctaca agttcagaaa
    32521 tattaattga aaaagactta gctaacagcg agatattcaa aagacttata tttctttctc
    32581 cgcgttcaac tgatccaata taagtacgat gtaatccaac tttttctgct aattcttctt
    32641 gtgaccaacc attaagtaaa cgataatgtt ttacacgctt accaaactca actaattcat
    32701 tttccataat ctgtattatt ttaataagtg aatactttaa gtctacactc tataagtagc
    32761 acacttaaca aaccatatcc tactgcgtga ataccatagc aaactgtagc ctgcatcaaa
    32821 ccgcgtgcat gccttcgggc acacactcgg tctttctaaa ccactccccc tttccaccca
    32881 aaccaaaggt cgtctgaaac ccattttcag acgacctttt gccactttgc aaagcaagta
    32941 ttcccaccac cctctcccca aatatcgccc aaaccagtaa acttcccata tttcaacaac
    33001 ttcttgaaag ccaaccatgt ccggtatcta cctaccccac ctatttccgc cccatatcgc
    33061 cgaacgcggc cttttgtatt ttcagcaggg caaggttcgc gatgtccgaa aaacttccgc
    33121 cgggcgttat cgggcctagc gctgtttgct gccggcggtc tgaataatgg tggcgtcaac
    33181 gacggcagcg gatgctttct ctacttttag gtttttttcg gtcagttggc ggttaatcag
    33241 tttgagcaat tcggacaggg tgtcgtcttg cgccagccag ttgcggtaac ggcataaggt
    33301 gctgtaatcg gggatgctca gttcgtcaaa gcggcaaaac aggttgaagt cgatgcgggt
    33361 gatgaggctg tgctcgagtt cgggatcgga gaggctgtgc cattgtccga gcagaacggc
    33421 tttgaacatg gacaacagcg ggtaggcggg acgaccgcgg tggtctcgga ggtaacgggt
    33481 tttttgacgg ttcaggtatt gttcgatcgg ttgccaatca atcacctggt ccaacttcaa
    33541 tagtgggaaa cggtcgatgt gttt
//
LOCUS       DAXHMX010000005        31679 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHMX010000005 DAXHMX010000000
VERSION     DAXHMX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.005"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(213..860)
                     /locus_tag="ACFM4S_000165"
     CDS             complement(213..860)
                     /locus_tag="ACFM4S_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6049928.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(857..1225)
                     /locus_tag="ACFM4S_000166"
     CDS             complement(857..1225)
                     /locus_tag="ACFM4S_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6049929.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1235..1906)
                     /locus_tag="ACFM4S_000167"
     CDS             complement(1235..1906)
                     /locus_tag="ACFM4S_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6049930.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2071..2790)
                     /locus_tag="ACFM4S_000168"
     CDS             complement(2071..2790)
                     /locus_tag="ACFM4S_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6049931.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(2874..4187)
                     /gene="hpnE"
                     /locus_tag="ACFM4S_000169"
     CDS             complement(2874..4187)
                     /gene="hpnE"
                     /locus_tag="ACFM4S_000169"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6049932.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(4266..5138)
                     /gene="hpnD"
                     /locus_tag="ACFM4S_000170"
     CDS             complement(4266..5138)
                     /gene="hpnD"
                     /locus_tag="ACFM4S_000170"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6049933.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTILYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDSKTLEYADKMGLALQLT
                     NIIRDVGEDARNGRIYLPMEEMRRFDVPASVILQCRPTENFTELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5309..7171
                     /gene="hscA"
                     /locus_tag="ACFM4S_000171"
     CDS             5309..7171
                     /gene="hscA"
                     /locus_tag="ACFM4S_000171"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6049934.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7248..7904
                     /locus_tag="ACFM4S_000172"
     CDS             7248..7904
                     /locus_tag="ACFM4S_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6049935.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISEVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAESGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYFRKNGRRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAESRKPR
                     "
     gene            7940..8701
                     /locus_tag="ACFM4S_000173"
     CDS             7940..8701
                     /locus_tag="ACFM4S_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6049936.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFEYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            8756..9097
                     /gene="fdx"
                     /locus_tag="ACFM4S_000174"
     CDS             8756..9097
                     /gene="fdx"
                     /locus_tag="ACFM4S_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6049937.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9384..9971)
                     /locus_tag="ACFM4S_000175"
     CDS             complement(9384..9971)
                     /locus_tag="ACFM4S_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6049938.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            10184..10381
                     /gene="iscX"
                     /locus_tag="ACFM4S_000176"
     CDS             10184..10381
                     /gene="iscX"
                     /locus_tag="ACFM4S_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6049939.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10449..10850)
                     /locus_tag="ACFM4S_000177"
     CDS             complement(10449..10850)
                     /locus_tag="ACFM4S_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6049940.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            11061..12020
                     /locus_tag="ACFM4S_000178"
     CDS             11061..12020
                     /locus_tag="ACFM4S_000178"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6049941.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12088..13398
                     /gene="tilS"
                     /locus_tag="ACFM4S_000179"
     CDS             12088..13398
                     /gene="tilS"
                     /locus_tag="ACFM4S_000179"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6049942.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            13472..14257
                     /locus_tag="ACFM4S_000180"
     CDS             13472..14257
                     /locus_tag="ACFM4S_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6049943.1"
                     /translation="MRHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLARYKGRVFATALREEKQAVLYGEDLCGPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            14267..14407
                     /locus_tag="ACFM4S_000181"
     CDS             14267..14407
                     /locus_tag="ACFM4S_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049944.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LI"
     gene            14392..14544
                     /locus_tag="ACFM4S_000182"
     CDS             14392..14544
                     /locus_tag="ACFM4S_000182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049945.1"
                     /translation="METHLIVQYRGGGGFGLGVVEMVLTDIAQNRANGIRTNKEFGKK
                     FVKSKN"
     gene            14726..15079
                     /locus_tag="ACFM4S_000183"
     CDS             14726..15079
                     /locus_tag="ACFM4S_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049946.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            15091..15396
                     /locus_tag="ACFM4S_000184"
     CDS             15091..15396
                     /locus_tag="ACFM4S_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049947.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSDAGIRPH"
     gene            15458..16834
                     /gene="mpl"
                     /locus_tag="ACFM4S_000185"
     CDS             15458..16834
                     /gene="mpl"
                     /locus_tag="ACFM4S_000185"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6049948.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTVVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVGIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     GKLLDALR"
     gene            17041..18093
                     /gene="bioB"
                     /locus_tag="ACFM4S_000186"
     CDS             17041..18093
                     /gene="bioB"
                     /locus_tag="ACFM4S_000186"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6049949.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(18171..18806)
                     /locus_tag="ACFM4S_000187"
     CDS             complement(18171..18806)
                     /locus_tag="ACFM4S_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6049950.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKV
                     RKLITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            complement(18930..19169)
                     /locus_tag="ACFM4S_000188"
     CDS             complement(18930..19169)
                     /locus_tag="ACFM4S_000188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049951.1"
                     /translation="MGVCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVSRILSVHIAGI"
     gene            19156..19389
                     /locus_tag="ACFM4S_000189"
     CDS             19156..19389
                     /locus_tag="ACFM4S_000189"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6049952.1"
                     /translation="MQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAAGGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19452..21311)
                     /gene="ilvD"
                     /locus_tag="ACFM4S_000190"
     CDS             complement(19452..21311)
                     /gene="ilvD"
                     /locus_tag="ACFM4S_000190"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6049953.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21484..23253)
                     /gene="cysI"
                     /locus_tag="ACFM4S_000191"
     CDS             complement(21484..23253)
                     /gene="cysI"
                     /locus_tag="ACFM4S_000191"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6049954.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGGGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVE
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23280..25094)
                     /locus_tag="ACFM4S_000192"
     CDS             complement(23280..25094)
                     /locus_tag="ACFM4S_000192"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6049955.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25091..26377)
                     /locus_tag="ACFM4S_000193"
     CDS             complement(25091..26377)
                     /locus_tag="ACFM4S_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6049956.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSVLTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26416..27339)
                     /gene="cysD"
                     /locus_tag="ACFM4S_000194"
     CDS             complement(26416..27339)
                     /gene="cysD"
                     /locus_tag="ACFM4S_000194"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC6049957.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMENRKKEGYF"
     gene            complement(27377..28111)
                     /locus_tag="ACFM4S_000195"
     CDS             complement(27377..28111)
                     /locus_tag="ACFM4S_000195"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6049958.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLNRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(28127..29587)
                     /gene="cysG"
                     /locus_tag="ACFM4S_000196"
     CDS             complement(28127..29587)
                     /gene="cysG"
                     /locus_tag="ACFM4S_000196"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6049959.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELEVLARHHIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGTLKAALI
                     AERLQQYGRPPDTPAAVISHGTQSTQSTAIGTLANLARLAENAPKPALIVVGEVVELH
                     EKLAWFGENAKKENNPAEHAYFALDGLGTGQEQQAA"
     gene            complement(30037..30693)
                     /locus_tag="ACFM4S_000197"
     CDS             complement(30037..30693)
                     /locus_tag="ACFM4S_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6049960.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            30758..31648
                     /locus_tag="ACFM4S_000198"
     CDS             30758..31648
                     /locus_tag="ACFM4S_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6049961.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHHAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT         6826 a         8291 c         9196 g         7366 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taccatacaa ccacgccgga attaagttta
       61 aatttgaata aaaggttcgg gttctgcaaa atacagaacc cgaaccttgt tcggatattg
      121 aaaccggccg cccgattttg ggcggtgcgg cttgcaagta tcaagattcg catatgccgt
      181 ctgaagctcg gagaggttca gacggcatat gcttatttgg gctgctcttc gacgaatctc
      241 ggacctttca agatgccgtt gtgagaatag ggcgacagca ggttgtatgc ggcggttttg
      301 gaaacctgat aaccgcggtc ggtcaggctg ttggcaatct gattgaccac tgcgctgacc
      361 aaagccccca acaggccgct gttgctgttg ttgctgcctt cgcggatgct ggccgaaccc
      421 gaccacaact cttttccgtt gcgggaatcg accagccgtg ctttggcgga tacggtcgtc
      481 acgctgtcta aaatttgata tgaagtgccg tattcggtaa ccgtaatgta caaaaccgca
      541 tcattgccga aaatctgatg cagtttttcc ggccggacgg cgtgaatatc ggcggcattg
      601 gtcaagccgt tttgtttgaa ggtttcctcc acgactgcgg cggggaagac gtaatagccg
      661 gcttcggaaa gcggcgcggc ggtcgaagcc agtacacccc atgttccgtt gacatcgggc
      721 gattcgttca gcggcggaac caccaaaatt gaagccggtt tgctttcctt gaatgacgtg
      781 tagtcgaaat cgggcgcttt ttgaacttgg caggcagaca gcgccaacac ggcggcaagc
      841 cctaaaatca aaggtttcat cgcttgcctc ctttaccggt tttcatcagg aagtccataa
      901 atacgcccga ttcgggaaac agccttttct cttcttcaaa ctggcggaac gcgccctctt
      961 tgtctcccga acgggaaagc agcagtccca gatgggcgtg cgcacccggg gcggcattca
     1021 tttttttgtt gccggcttcc acaaagtatt tttccatctt ttcggtctgc ttgcccaacg
     1081 aagtgtcgtc gtttttcaaa ccttcataga cggtatcggg atagccgccg taataataca
     1141 gggatttttg cccgttgccg ccgcaggcgg tcagagccaa gaccgccgca cacagcgaca
     1201 aacggctcaa ggttttcgga ttcatcattt ctccttaacg gttgggttgc catgcgccgt
     1261 tgtcaacagc ctgaaccagg ctgttgacgg cttcgcggat tgccaagtct aaaactttgc
     1321 cgttcaaagt cgcatcgtag ccggaagtgc cgccgaaacc gatgatttca cggttggaaa
     1381 gtgcgtattc gcccgcgccc tgtgcggaat agacgatttc ggaagtattg acgttgacga
     1441 tattcagagc cacttttgca taggcgattt gcgatttgcc gcgacccaaa atgccgaaga
     1501 gctgatgatc gccgacatct ctgcgtccga attcggttac atcgccggta acgacataat
     1561 ctgcgccttt caggttatgc gctttgccgg aaatgccgga ttcctgtttt aatgcgttca
     1621 aattggtgcg gttcagtacg ttgaagcggt tggtctgttg caggtgcgtt actagaatgg
     1681 tttttgcctg gctgcccaaa cggtcttccc cgtcggagaa aatgcctttt tggaagctgg
     1741 agcggttgtc gaatgttccg acggaaatcg gggtacgaac accgtgatat tgcgtattgt
     1801 aggaggcgac tttctctacc tcgagactgc gtgaggattc ggtcgcacag ccggtcagtg
     1861 aaacggcagc ggcggcaagg acaacggcgg tggaaacggt tttcataaaa tttaccctaa
     1921 ggtcaagtta aggaaataac gggttatcat tattgtcctt atgtaaattt aagtcaaggt
     1981 gtttgtctgt gcgggacgga tgcgcgcgga aggtacggat atttttcaaa cggaatcgcg
     2041 gcgcgggcgg gatgcctgcc ttttccggat ttagaggtaa acgatttcgc cgcttcgccc
     2101 tttgctttcg gtacttgccc accagacaaa tgcgggcagc acgtccccgt agcttttgcg
     2161 ttcgcttttg gcttcgcccg gatgggattt gatgcgttgc ggggaattga tgggtccggg
     2221 gacgaggacg ttggcgcgca ggttgccgaa gcgttcccat tcgtcggcgg cgactttgca
     2281 caggtagttc aacgcggctt tggacgcgcc gaagccgccc cagtaggctt tgggtgtttc
     2341 gccgtggctt tcgccgacga agatgacgga cgcgtcgggc gactgcttca gcagcgggaa
     2401 cagggcgcgg gtcagcccca taggtgcgac ggtgttgatg cggtattggt tgacccattc
     2461 ggcgacggtt tggaaatcca gcggcgagag ggcgtaaaaa tagccggcgc agtggacgat
     2521 gccgtccagt ttgccttgcg tggcttcggc aatggtggcg gcgaactgat tgaattcttt
     2581 ttcttccgca ctcatcaggt cgaaacggat ggcgaagggt tcggggtgtc cggcttcgac
     2641 aatcgcgtca tagacttttt ccagtttttt ctgatggcgt gccaccagaa tcacggttgc
     2701 gcctgccgcc gcataggctt tggcgacctg ttcgcccaga ccttgcgatg cgccggtaac
     2761 taagatggtt ttgttggaca gtgtcgccat actttttcct ttttggtggt cggtcggggg
     2821 tattttagcg ttttgccgca ccttgtaaag cgtcccgata ccagccggcg ttttcagacg
     2881 gcatcgctca ggctttgcag gcaggtttcc gccgccgcga aacctgattg tacggcggct
     2941 tcgagcgtgg cggggtagtc tgggtggagg tagtcgccgg cggggaagat gcggtgccgg
     3001 tgcagccacg acaaatccgg cggcggggca tcggctgcgg ttgtggcgcg tttttcggtg
     3061 atgacgcgca cggcttcggg ttcgcccaaa tgcggaagga tgcgtttgag gtcggcgtga
     3121 actttgtccg cccacgctcg gtttgtaaac gcgccgacgc ggtcggaaac gctgatgacg
     3181 gcggacactt cgttttcagg cagtccgagc ctgccccggc aaagcagcca ttgcaccgtg
     3241 ccgtcggcaa ggccggtcag cggggcgggc aggcggacgg gttcggcgta gcgcagatag
     3301 acggtggtga tggcgtggta gcgaaggttt tgatatgccg tctgaacgtg ttcgggcgtg
     3361 ccttcgggca ggagcgcggc ggcgtggtag ggcgcggtgg cggggacggc ggcatcgaaa
     3421 gcttcgccgt tgacgagcac tttcccgtcc gggagggtgt tcagacggca tacgcgcgtt
     3481 tcgaggcgga tgtccgcgcc gagccgttga agatccgcca aggcgggttc ggcgacgatt
     3541 gcgcccaaat cctgcttggg taggagatag tcgctgccgg attttttcgt cagcacgccg
     3601 tcggacaaaa cgttgcacaa cacgcgcagg cttgcggttt ccaaaggcgt gttgagcgcg
     3661 ccccaaacca agggctgcca aaactgcatc acggcggcac gcggcacgtt ccgctgtttc
     3721 agccattgcg ccactgtcgt gtcgggctgt ccgaggcgtg cggacttctg caaatcggac
     3781 atatcggcaa gcagtttggc tttgaatgca gtcggtgcac gccgggcaag cagcacgccg
     3841 cccaaaatat gcagcggcgc gggcaggggg agggcgcgga actgcaaacc gccgtgcata
     3901 tgccagtgca gcggtacgcg caaaaaggcg gcacggggat ccgaaccgat ggttttcatc
     3961 aggcgcaaca cgccccggta tgcgccgagc aaaatgtgct gcccgttgtc caaaaaaccg
     4021 aaaccgtcgg tatttccggc cagtgtgcgc gccctgccgc ccgcctgccg gccggcttca
     4081 aacagggtaa cgtcggcgtg ccgcgccaag gtgacggcgg cggacagtcc tgcccagcct
     4141 gcgccgatga cggcgatttt cgggcgcgga tgcggcgtgt tcatcattta ttcctccaat
     4201 ggttgaaacc ccgtttttcg gggcggcggg ggttgggttt tcctgaaggg cgggcggtat
     4261 gcgccttatg cccgttccgg cgtgccgggg cgcggtttga atccgaataa ccaggttttc
     4321 agggcaatgc gttttttgcg cggcgaaggg agggcgattt tgtatttgag gacgttttgc
     4381 gcgccgtctc ggtcgatttc gtttaaaagc gcgtaataaa ccgccgccat aaccagtccg
     4441 actttttggg cttttttgtc ggcatcaggc agcagcgata cggcttcacg gtaggtttca
     4501 cgcgcacgct ttatttggaa tgccatcaat tcggtgaaat tttctgtcgg ccggcactgc
     4561 aaaatcacac ttgcggggac atcaaaccgc cgcatttcct ccatcggcag gtaaatccgc
     4621 ccattacggg cgtcttcgcc gacatcgcgg atgatgttgg tcagttgcag cgcaagtccc
     4681 attttgtcgg cgtattccag tgttttgctg tctgaaaacc ccaaaatccg cgcaatcagg
     4741 cagccgacta cgcctgcgac gcggtggcaa tacagtttca attcctcaaa actgccgtaa
     4801 cgcgcctgaa ccaaatccat ctgcatcccg tcgattaagg cttccagttc atatttcggc
     4861 agcttgaagg tttccttaac ttgccgcaag gcctgattga cggggtgttc cggcatcgcg
     4921 ccgccgaata ccttgtccaa atcgccgcgc caccagttca atgttgcctg tgcaacatcg
     4981 gggttggaac attcgtcaac cacatcgtcc aattcgcggc aaaaggcata taaaatcgtt
     5041 accgcatccc gtttttcctg agtcaggaaa cggaagcccg ataaaaaact ggagcggctt
     5101 tcttctgctt tttgacggca atagtcgagt cctttcacga tttatattcc taatgatggg
     5161 cgggaaaggc ggattttatc ggcatttggc ggtagagggc aatttcggcg gcacgaccca
     5221 atccttagcg gtttgctcaa ctatcggcgc gaattctgtt aaaatgccgc tttccttcct
     5281 ttacacaccg caccgacagg cagaatttat ggctcttttg cagatttcag aaccgggtat
     5341 gtccgccgcc ccgcaccggc accgtttggc agcaggcatc gatttgggta cgaccaacag
     5401 cttggttgcc accgtccgca gcggcagtgc cgcctgcctg cccgatgccg aagggcgcgt
     5461 taccctgcct tccgtcgtcc gctatctgga aaacggcggc attgaagtcg gcaaaaccgc
     5521 cctgtccgcc caaaaaaccg acccgctgaa caccgtcagc tccgccaaac gccttatcgg
     5581 gcggacgctt gccgatctgc atcaaaatac gcactacctg ccttaccgtt tcggcgacaa
     5641 tcaacgcgtt atcgaattgc atacgcggca gggggtgaaa acgcctgtcg aagtgtcggc
     5701 ggaaatcctc aaaaccctta aatcgcgcgc cgaagaaacc ttgggcggcg atttggtcgg
     5761 cgtggtgatt accgtccccg cctatttcga cgatgcccaa cgccaggcca ccaaagatgc
     5821 cgcgcggctg gcgggtttga acgtattgcg cctgctcaac gaacccaccg ccgccgcaat
     5881 cgcctacggg ctggacaacg cctcggaagg cacgtttgtc gtgtacgact tagggggcgg
     5941 cacattcgac gtatccgtat tgcaactgac caaaggactg tttgaagtca aagccaccgg
     6001 cggcaacagc gcgttgggcg gcgatgactt cgaccatcgt ttgttctgct acctgctcga
     6061 acaaaacgga ctctcccaac tcaacgaaca agacagccaa ctcctgcttt cgctcgtccg
     6121 cgccgccaaa gaacaattaa ccacgcaaac cgaagcgcgc attcaggcga cgctttcaga
     6181 cggcatggca atcgacacaa gcatcagtcg cgccgagttc cacaacctga cgcagcattt
     6241 ggtgatgaaa acgctcgaac cggtcaaaca ggcgttgaaa gatgccggtg tcggtaaaaa
     6301 cgaagtcaaa ggcgtaatta tggtcggcgg ttcgacccgt atgctgcacg tccagcaggc
     6361 agtcgccacc tttttcgggc aaaccccgct gaacaacctc aaccccgacg aagtcgtcgc
     6421 gctcggcgca gccatacagg cgaacgtcct cgcaggcaac aaagccgacg gcgaatggct
     6481 gctgctggac gttacgccct tgtcgctcgg tttggaaacc tatggcggct tggcggaaaa
     6541 aatcatcccg cgcaattcca ccatccccac cgcgcgcgcg caggacttta ccaccttcaa
     6601 agacggtcag accgcgatga cgatacacgt cgtacaaggc gaacgcgaac tggttgccga
     6661 ctgccgcagc cttgccaaat tcaccctgcg cggcattccg ccgatggcgg cgggtgcggc
     6721 gcgtatccgc gtaaccttcc aaatcgatgc ggacgggctg ctgtccgttt ccgcccaaga
     6781 acaaagtacc ggcgtacacg cgcaaatcga agtcaaacct tcctacggtt tggatgacgg
     6841 cgcgattacc cgaatgctta aagacagtat ggacaacgcc gccgaagata tggcggcacg
     6901 cgcccgcgcc gaagccgtgg tcgaagccga aagcctgacc gatgccgtca acgccgccct
     6961 cgagttggac agcgatttgc tggatgccga agaattgcaa cagattcggc aaggtatcgc
     7021 cgatttgcaa ggccgtctga aagacggaaa agccgaagac atccgcgccg ccgccgccaa
     7081 actcggcagc atcaccgaca atttcgccgc caaacgcatg aaccgcaaca tccaacgtgc
     7141 gctgacaggc cagagtgtcg ataatatttg atacttaaac ggtttcagac ggcatagaga
     7201 taacctgatg ccgtctgaag gctcgaaaac acttgaaaaa catcgatatg gaaaagtcag
     7261 gcattgtcta tctgatgaaa accgtcatca agggcgtata taaaatcggc atttcggagg
     7321 taagcaattt tgaaggcaga atgcgccatt tggaaaacaa cggctatgcg aacgttgccg
     7381 gattggagcg catcctcgcc gtcaaaaccg acaattacaa agaaaaagaa aacctgctcc
     7441 atgaaatttt cagcaaaagc aggataggcg ataccgaatt gttcgccgtg gacgaaaacc
     7501 ttgtaaaacg tttgttttta tcgcttcgcg gcgaaatcgt gttcccgaaa aacgaaacgg
     7561 cggaatcgga atttgaaaaa agcgtccacg aacgcaggca ggaagggaat gccgaatccg
     7621 gccgcaaaca actgcttgat ttggtacggc gcggacaccg ggaataccct tacgcgctgc
     7681 cccggctttt ggcgggcgcg gcatcctaca agccgaaaaa atcgaaaatc cgccttttta
     7741 aagaagcata tttccgcaaa aacggcagga ggctgacgga cgaaattgca gacggcatcc
     7801 atatttacac ctgtttttcg cgggcggatt tggaaaaagc ctattccgaa tatttggaac
     7861 ttttcaaatc cgaatcggat gccgaaagca gaaaaccacg gtaaggtgca aacagatacc
     7921 gtacacgttg aggagcagta tgatgggcga ttccgtcatt tattatgtag aacaggcaga
     7981 caaaccggta aaccgtgccg gcgaacgcgc ccgtaaaaca ttcgaatatt tttggcgcga
     8041 gcttttttgg gaacgccgcc gcatcattcc cgccttagat tttgccatgg tcaaagcccc
     8101 ttttttccaa gacggcgaag acggcgaaat ttgcgaacat atgtggatcg acgatattta
     8161 ttttgacggt ctttacattt atggcgtgct gaacaatgaa cccggcgaac tgaccaatgt
     8221 cgaacaaggc gaaagcgttt gcgttccggt tgacgacatc agcgactgga tgttcgtgtg
     8281 caacggcatt ccctacggcg gctttaccat acaggcaatg cgtggacaga tgacggaaga
     8341 ggagcgtacc gaacacgatg ccgcatgggg aatcgatttc ggcgatcccg ggcaggtatt
     8401 gctggtgtat gaagaaaaag aacatcccga aaatctggaa gagcatccga tgtgccggaa
     8461 ctgtattgac gattttcggc aacagttgtc ccaaaatccg gattttctgc atgagcagga
     8521 cgaagacggc tatacgccgc ttcatcatga agccatcgca ggaaatgcac ttatggttca
     8581 agccatgctt gaatacggcg caaatcctgc ctcaaagaca tcggaaggct ataccgccct
     8641 cgattttgcc cgcctggcgg gctggcaaaa tgttgccgac ctgctcgaac cgcgacatta
     8701 ggcggacagt tttccgaaaa cgaacacaaa cactttttac agaaagacaa taaaaatgcc
     8761 caaaatcacc gtacttccac acacgacatt atgccccgaa ggcgcggtta tcgaagacgc
     8821 gcccgaaggc aaaaccgtcc ttgacgtgct gctcgaccat gatatcgaag tcgatcacgc
     8881 ctgcgaaaaa tcctgcgcct gcacaacctg ccacgtgatt atccgcaaag gtttcgacag
     8941 cctggaagag ccgaccgaat tggaagaaga cctgctcgat caggcttggg gtttggaagc
     9001 cgattcgcgc ctgagttgtc aggcggttgt cgccggcgag gatttgattg tggaaatccc
     9061 caaatacacc atcaaccacg cgcgagaaga acactgaaaa caggccgcct gaagccggca
     9121 cgcttcagac ggcattgttg cgcggataag gcgcaatcgc ccgaaaacag gcgttcgtac
     9181 aggcggaact ttcgattcta tagtgaatta acaaaaatca ggacaaggcg gcgagccgca
     9241 gacagtacca atagtacggc aaggcgaggc aacgctgtac cggtttaaat ttaattcact
     9301 atatattgat ttttatcggt tttctgacgg aacggattgg tgcggcatcc gaggcggatt
     9361 actcgggcgc gatgcacggg tatttatcgg ttttgcagcc ggaaaaaccg ccggcgggtt
     9421 actttcaggg cggcggtcgg gcggtatgcc ggatagcgtt ccactttcgc ttctatattg
     9481 attttgattg gttttctgac ggaatgatcc gatgcggcat ccgggacggc ttgtgtttct
     9541 tcctgcccgc ctgccggata ttcctgtact tgcacgaaac cgaaagaggt ggcggtgcgg
     9601 cggacaagct cgagatagcg tccttcaagc tccggacagg cggcggacac gctttctgcc
     9661 gtaaccgtga agccgccgcc cgacccggca aggcgttccg caatctgcgt gtagatgcgc
     9721 caacgttggg cgcgcaacat tgccgcatcg caaccggcag ccgctttatc caaggcaatc
     9781 agaccgcgcc tgctccggta gtgaaggcaa accgttactc tggagaggat atgttgcccg
     9841 tccaatattt gatacagttt gcgtatcagc agaatcaggc ggtcaaactc ctccatctcc
     9901 gacagggaat caggatggtc ggaagcggta taaaccagtt tggacaattt tgcggcaaac
     9961 atattgttca tcaatcttcc ttgtcggttg aaaccccgct cttcggggcg gtagaatcag
    10021 atttgtttgg gagggacaac tcctcccaga tcaggacgac acataggctg gtgcttgatg
    10081 tgttgtccgg cgagttgaaa cattcagcca tcctcaaggt gcggcagttt tgccgaaaca
    10141 tattctacac ggcttcaatg ccggacgata aaaggaaatt catatgaaat ggaccgacac
    10201 ccggcgtatc gccgaagaac tctatgacct gcacggcgaa accatcgatc ccagaaccgt
    10261 gcgctttacc caactgcgcg acctgattat ggcattgccc gaatttgacg acgaccccgc
    10321 ccgttgcggc gaacgcatcc tcgaagccgt gcagcaggca tggatagacg aggcggaata
    10381 agtttcggga atgccgtctg aaatgcggcg gtacgcggtt cgtgcttctg tttgcagcgg
    10441 gaatggtttt accagtctcc ttttttcagc ctgtccagtt ggcggcggtc gcgcttggtc
    10501 ggtctgccgt cgggataggc ggaagtgatg cggctgaatt ggtcgagctg tttgcgctct
    10561 tccctcaatg ctgccgtttt tgcgtcttct tcatacagaa gccgcgcctc ggatgccggg
    10621 cggcgttggt ggttcaaacc tttaaccttg attttatagg gaagggaatt gagcgtcagg
    10681 tcgataatat cgccgatgtc tatggtttta ctgtttttga ccttcgagcc gtttacttga
    10741 accctaccca gttcgatgtg cttttgcgca agggaacggg tcttgaaaaa acgtgccgcc
    10801 caaagccatt tgtccagccg catggcggaa gaatcgtgct tgtctttcat acgattttgt
    10861 ttgaaataat tgaatttgtt tcgagtttag cataagatac gccgcctata actagtatat
    10921 atgcactaat ccactgtttt ccatgctgtc cgaacacaaa aagagggtgt ggaaaagccg
    10981 ttttggacaa taaattaact gcggaatatg cacaaatagc gtatgatagc ggcagaatct
    11041 gttgatgaga gcttcattct atgaaacctg tttttttgga ttttgaacaa cccatagccg
    11101 aactgaccaa caaaatcgat gagctgcgtt tcgtccaaga cgagtctgcc gtcgatattt
    11161 cggacgaaat acaccgtttg cagaaaaaaa gcaacgacct gaccaaatcg attttcagca
    11221 aacttacgcc cgcccaggtt tcacaggttt cccggcatcc gcagcgtccc tatactttgg
    11281 attacattga tgcgctgttt actgattttg aagagctgca cggcgaccgc cattttgccg
    11341 acgatcatgc gattgtcggc ggattggcgc gtttcaacgg acaaagcgtg atggtcgtcg
    11401 ggcatcagaa agggcgcgac accaaagaaa aaatccgccg caacttcggt atgccgcgcc
    11461 ccgagggcta ccgcaaagcc ctgcgcctga tgaaaacggc tgagaaattc ggcttgcccg
    11521 tcatgacctt tatcgatacg ccgggcgcgt atcccggcat cggcgcggaa gaacgcgggc
    11581 agtcggaagc catcggcaaa aacctgtacg aactgacgcg cctgcacgtc cccgttttgt
    11641 gtaccgtcat cggcgaaggg ggctcgggcg gtgcgttggc ggtcgccgta ggcgattacg
    11701 tcaatatgct gcaatactcg acctattctg ttatctcccc cgaaggctgc gcttcgattt
    11761 tgtggaaaac cgccgaaaag gcggcggatg cggctcaggc tttgggcatt actgctgacc
    11821 gcctgcaaaa gctggacttg gtcgataccg tcatcaaaga accattgggc ggcgcgcatc
    11881 gggatttcgg gcaaaccatg aaaaacgtaa aagccgtttt ggaaaaacaa ctgcacgaag
    11941 cgcaaagcat cccgcttgcc gatttgcttt cgcgccgttt cgaccgcatt atggcttacg
    12001 gcaaattttc ggaacaataa ttcaggtaga acaagcagca agcagtttgt ctgaaactgc
    12061 ttgctttttc tttatcggga cggaaccgtg ctgactttag atgcgtttga gcaatgcttg
    12121 aaggattgtt ttcctcaagg tctgaatgga aaaaaaacag cagtggcatt aagcggcggc
    12181 ttggattccg tcgttttgct gcatctgctt gtccgcgccg gaaaaaaggg cggttttatt
    12241 ccggatgcat tgcatatcca tcacggcttg agtccccgtg ccgacgattg ggcagatttc
    12301 tgccaaaact attgcgatat gctcggggtg gggctggaaa cggttaaggt ctgcgtggaa
    12361 aaaaacggtt tgggcatcga ggcggcggcg cggcaaaagc gttatgccgc gtttgccgaa
    12421 aaaggctttg acgttttggc gttggcgcac cacagggacg atcaaatcga aacctttatg
    12481 ctggcagtcg ctcgcggcgg cggtttgcgc gctttggcgg ctatgcccac cgtccgccct
    12541 tttggggaaa aaggcatcat ctggcggcct ttgctgcctt tttcgcgcca agacatatgg
    12601 gattatgccc aaaaacacgg tttgccgaat atcgaggacg aaagcaatag cgatacggct
    12661 tatttgcgaa accgcttccg gcaccgtatt ttgcccgaac tttcggcgca gattccccat
    12721 ttcgggcggc atgtactgaa caatgtccgc gctttgcagg aagatttggc tttgttggac
    12781 gaaatcatcg gtcaggactg ccgttgggtc tgtgaggaag gcagtttcga tacggtgcgt
    12841 tggcaggcgt tttccccacg ccggaaaacc catattttgc ggtatttttt gaaggaaaac
    12901 ggcatcccca tgccgaatca gaatgccctt gccgacattg cccgggtttt gacggaggca
    12961 aaaaccggac gttggaactt gcaaggcttt gaattgcatc attatgcagg caggctgttt
    13021 gtgttccgac tggaaaaaac ggataaactg cggtttttga aagacaggca gataagcgga
    13081 aatttaaggg aaatattgac ggggcaggga tttgttttaa aacgtcatcc gtttgggctt
    13141 cctgagcatc ttttggagca ggacggaatt ttgaggacgg tagcggtatc ggatacgttg
    13201 gctgtcggcg gcatccataa ggatgtgaaa aaaatccttc aggggaaacg ggttttgcct
    13261 gtcctgcgcc caatttggcc gcttgttgcc gacagcggaa accgtccatt ggcgttggca
    13321 aactgttgtg cggattttca aatctcggtt tcagacggca ttttgcccgt ccatcctgac
    13381 tttcccattt tattttgata atatcgcaaa cagatttcgg cggcgttcag tcgggtattg
    13441 tccggttgca tatttctaaa aggcttgtga agtgagacac atcagttcga ccaataatga
    13501 acacatcaga cacctgcacc gcctgttgtc gcaaggaaag ttcagacggc aatacgccca
    13561 aaccgttttg gagggcgtgc acctgcttca ggttttcctg caatccggcg ggatgccggt
    13621 cggggtatat attcccgagg gcaaaatgcc gtctgaagaa atccgcaggc tgagggcggt
    13681 tgtgccggaa ggcaggattt tttccgtttc agacggcata ttgaaaaaaa tcagtagctt
    13741 gagttgtgca gacgatatac ttgcactgat tgatatccca ttgggcggaa ccttgccgga
    13801 caaaggcgac tgtgtggttt tggacggcgt gcaagatccg ggcaatgtcg gcaccgtctt
    13861 acggagtgcg gcggcggcag gggtaggtac ggtcgttttg ggcaggggtt gcgcggacgc
    13921 gtggtcgccc aaggtattgc gcgcggggat gggcgcgcat ttcttgtcgg gcatttatcc
    13981 gcaggcggat ttggaaatat ggttggcgcg ctataaaggc cgtgtgtttg ccaccgcctt
    14041 gcgcgaggaa aagcaggcgg ttttgtacgg cgaagatttg tgcggaccga cagcctgggt
    14101 gtttggcaac gaaggcgcgg gggtcggtaa agcggttttg gacagggcgg acaagtgtgt
    14161 caggattccg atgcacgatg caaccgagtc tttaaatgtc gcgatggcgg cgacaatctg
    14221 cctgtttgaa cagatgcggc agcggtcggc gtattgagga aaagaaatgc cgtctgaaaa
    14281 aatctattac ggcgtattga ttttcttatg tatcgcttct atgctgctgt cgccgttttt
    14341 ttatgcgggt gctttgaagc ccaagaaggc ggcattgcgg aaggacgggc agtggaaact
    14401 catctgattg tccaataccg tggcggcggc ggttttggct tgggtgtggt ggaaatggtt
    14461 ttgacagata ttgctcaaaa tcgtgctaat ggaatccgaa caaataaaga atttggtaaa
    14521 aaatttgtta aatcaaagaa ttaaagtttt gtggaaaaca aaacagctct aagcaaatag
    14581 ggcgtttgtc ggtaaatacg gaagagttgc ggcattatcg ggcatcttta acaagtagtg
    14641 ccgtcttggc aggcaatcgg tttttatggg cagcttgcaa aatcgcggat ataaaattgc
    14701 gaatcggtta aagtgtgggg acgctatgaa aaattgcgaa tttttttatg acccgacaag
    14761 ggcaatctat gatagcggtg cagattactt aactagggaa aaacatagat tagtcgtaat
    14821 tgcaaatagt gcttgggggc tattacttaa tttatcttgt tattatgacg aggttttgga
    14881 aaagcggaaa ataccgttcg gcaaacagga aattgatgac gatatggaca aagtgtccgc
    14941 ccttaagcgg aagtttaaag atatttctga aatcaaagta ggggatggtt gggaataccc
    15001 gttcaattat gagcagggaa tgaaagaatt agatgaagtg ctattgaaat acattccctt
    15061 ttttgaagaa gaacgataaa ggaggttgat atgcgcgtat ctaaaataat tggaagtatg
    15121 ttgcttgtta cagcggttca gaccgtattt tcggcaaatg tttacgcgtg ccgccataat
    15181 ggtaaaacca gttacagcca aactccggga aaacattgta ccaacgcggg tttggggcgg
    15241 gaccgggtgt acagttcggt tagaccggca gtaaaagaca gggcggaaga cgcaggggtc
    15301 ggcgattatt cggacacggt gagggacgaa cacgtccaaa atccgaaagg gaatgcacag
    15361 aaagacggtt cggatgccgg catcaggccg cattgattga aaccgaatca gccatgacgg
    15421 cgttgaataa tagaatttga acgattaggg aactctgatg aaacacatcc acattatcgg
    15481 tatcggcggc acgtttatgg gcgggattgc cgccattgcc aaagaagccg ggtttgaagt
    15541 cagcggttgc gacgcgaaga tgtatccgcc gatgagcacc cagctcgaag ccttgggtat
    15601 aggcgtacac gaaggcttcg acgcggcgca gttggaggaa tttaaagccg acgtttacgt
    15661 catcggcaat gtcgccaagc gcgggatgga tgtggttgag gcgattttga accgcgggct
    15721 gccttatatt tccggtccgc aatggctggc tgaaaacgtg ctgcaccatc attgggtact
    15781 cggtgtggcg gggacgcacg gcaaaacgac caccgcgtcc atgctcgcat gggttttgga
    15841 atatgccgga ctcgcgccgg gcttccttat cggcggcgta ccggaaaact ttagcgtttc
    15901 cgcccgcctg ccgcaaacgc cgcgtcaaga cccgaacagc caatctccgt ttttcgttat
    15961 cgaagccgac gaatacgaca ccgccttttt cgacaaacgc tccaaattcg tacattaccg
    16021 cccgcgtacc gtcgtgttga acaatctgga attcgaccac gccgacatct ttgccgactt
    16081 gggcgcgata cagacccagt tccaccacct cgtgcgcacc gtgccgtctg aaggcttaat
    16141 catctgtaac ggacagcagc aaagcttgca ggatacttta gacaaaggct gctggacgcc
    16201 ggtggagaaa ttcggcacgg aacacggctg gcaggccggc gaagccaatg ccgacggctc
    16261 gttcgacgtg ttactagacg gcaaaacggc cggacgcgtc aaatgggatt tgatgggcag
    16321 gcacaaccgc atgaatgcgc tcgccgtcat tgccgccgcg cgtcatgtcg gtgtcggcat
    16381 tcagacggcc tgcgaagcct tgggcgcgtt taaaaacgtc aaacgccgca tggaaatcaa
    16441 aggcacggca aacggcatca ccgtttacga cgacttcgcc caccacccga ccgccatcga
    16501 aaccaccata gaaggtctgc gtcagcgcgt cggaaacgcc cgcatcctcg ccgtcctcga
    16561 gccgcgctcc aacaccatga aactcggcac gatgaagtcc gccctgcctg taagcctcaa
    16621 agaagccgac caagtgttct gctacgccgg tggcgtggac tggaacgtcg ccgaagccct
    16681 cgcgcctttg ggcggcaggc tgcacgtcgg caaagacttc gatgccttcg ttgccgaaat
    16741 cgtgaaaaac gccgaagcag gcgaccatat tttggtgatg agcaacggcg gtttcggcgg
    16801 aatacacgga aagctgctgg acgctttgag atagcccggg cgatgccgtc tgaaagccct
    16861 tcagacggca tcgtccggct gcgcggcaca aaggcggaaa aaccgtttgc cccgtatttt
    16921 caaacgcgtt acacttgccg ccgctgtttt cagccatttg attacccgca accgccgtca
    16981 ttgcgccggc ggtttgcctg tcagcgtcat tgcgccgctg taaatacgaa agaacacatt
    17041 atgaccgtat cccccgtcgc cttgcgccgt aagaccgagt gcaagcctca tcccaccgcg
    17101 cgctattgga aaaaatgcga tgtcgaagcc ctgttcggac ttcccttcct cgacctcatt
    17161 taccaagccg ccgaaatcca ccgccaaaat ttcaacccgc gcgaaatcca gctttccacg
    17221 ctgttgtcca tcaaaaccgg cggttgtccc gaagactgcg cctattgtcc gcaatcggcg
    17281 caccacaata ccaatctggg caaagagcag atgatggatg tggatgaaat cgtcgaaaaa
    17341 gccaaaatcg ccaaatcgcg cggcgcaagc cggttttgta tgggcgcggc gtggcgcggc
    17401 cctaaaccca aagacgtgga gacggtttcc gcaatcatca aagccgtcaa gggcttgggt
    17461 atggaaacct gcggcacgtt cggtatgctc gaagaaggta tggcggaaga cttgaaagag
    17521 gcgggcttgg attattacaa ccacaacctc gacaccgacc ccgaccgcta caacgacatc
    17581 atccacaccc gccaacacga agaccgaatg gacaccttgg gcaaagtccg caacgccggt
    17641 ttgaaagtct gctgcggcgg catcgtcggg atgaacgaaa cccgcgccga acgtgccggg
    17701 ctgattgcca gtctcgccaa tctcgacccg cagcccgaaa gcgtgccgat taaccggttg
    17761 gtcaaagtgg aaggcacgcc gcttgccgat gccgaagatt tggactggac ggaatttgtc
    17821 cgcaccatcg ccgtggcgcg gattacgatg ccgcaaagtt atgtccggct gtcggcaggg
    17881 cgcagcaata tgcctgaagc aatgcaggcg atgtgcttta tggcgggcgc gaactcgatt
    17941 ttttacggcg acaagctgct gaccacgggc aatcctgatg aggacggcga cagaatcctg
    18001 atggaaaagc tcaacctgta tcccttgcag tttgaaccgg aaggcgaggt cgccgaagtg
    18061 gaaaaagcct ctgggattaa agtggattat tgacgattga aaaatgccgt ctgaaacccg
    18121 gaaaaaggct ttcagacggc atttgtccgg acggcatttc caatatcttt ttaccggcgc
    18181 gtgatgctgc cgtcgggcga gacatccagt ccgttcccct tggggaaatc gctgcggttc
    18241 agataaataa tccgcccgat aacggttttc tcggggtcga ttttggggat tttatcgccg
    18301 acttgagtga tggggataat gttaccggaa acgaaccgcc cctgtttgtc ggtgataatt
    18361 ttgaaaatcg gcgcgatgcc gctgatgccg gaggtgttga ttgcgccgta ggtggcaaag
    18421 ttgccgccgc tgtaggagat gaagcggtcg cggtaaagtt cgacggcgcg agtaacgtgc
    18481 ggcccctgcc cgaatacgac atccgcgccg gaatcgacgg caagccgtgc aaactcgacg
    18541 acgttgcccc tgttttcccc atagaagatt tcggtatcga acggcaggtg ttccgcctgt
    18601 ttcccttccg cgccgccgtg gaacattaca atgacgatgt cggctttctg tttggttttg
    18661 gtaatcagtt ttctaacttt ggcataatcg ttcagtttga cggcggcaag gttgggggcg
    18721 aaggagacga agccgtatct tacgccgttt ttcttcagga tggcggtttc aaacctgttt
    18781 tcgatgcccg aatatttgat gttcaattcg tcaaggttcg ccctttatgc cgtttccgca
    18841 ccgcaaaccg ccggggtcaa gccctcggcg catcctgccg gaacggaatc cccgtgctgc
    18901 cgattgacgg ttcaaacccc gcgcccgttt caaataccgg cgatgtggac ggacaggatg
    18961 cgactgacga aaagacagcc gataccgttt ccattatcgg cgtgggcgac attatgctcg
    19021 gcagcaatta tccggtcgat tacctgcccg ataccaatat tctgaaaaac gtcgaatctg
    19081 ccttgcaaga cgcggacatt accgtcggca acctcgaagg cacgctgttt gacgaaggcg
    19141 gtacgccgaa aaaatgtgca aacccccaaa atatgctatg cattccgaac gccctccgca
    19201 tacgggcaat accttgccga cgcgggattc gactacctca gcttcgccaa caaccacagc
    19261 aacgacttcg gcgcgcaagg catcacggca acggcggcgg gcggcagctc ttttacatac
    19321 tcaatcgcgc taaagccgct gccgataacg aggccaaaat tgcggaaaat accgccatcg
    19381 cccagataaa tttgtccatc atcagacctt tactgttcag acgagacagc atttgccgca
    19441 cgttttgggg cttatctttc gatttgcgct acgtcgcgca ccgcgccttt gtcggcggaa
    19501 gtcgccatcg cgccgtaagc ccttaatgcg gcggagacgt agcggtcgcg gttttccggt
    19561 ttccacgctt tgctgccgcg tgcttccatt tcggcacggc gcgcggcaag ctcttcatcg
    19621 gaaatgacaa ggtggatgct gcggttgggg atgtcgattt cgacggtatc gccttcgtgt
    19681 accaaaccga tcgcgccacc ttccgccgct tcgggcgagg cgtgtccgat ggacaaacct
    19741 gatgtgccgc cggagaagcg tccgtcggtt aagagagcgc aggctttgcc gaggccttta
    19801 gatttcaggt aggaagtcgg atacagcatt tcctgcatgc ccgggccgcc tttcgggcct
    19861 tcgtagcgga tgatgacgat gtcgccggcg acgatttggt tgcccaaaat gccttcgaca
    19921 gcggcttcct ggctttcaaa cacgcgcgcg cggccggtga atttgaggat gctctcgtcc
    19981 acgcctgcgg tttttaccac gcagccgcgt tcggcgatgt tgccgaacaa gaccgccaaa
    20041 ccgccgtctt gcgagtaggc gtgtgccacg tcgcggatac agcctttttc gcggtcgagg
    20101 tcgagtgttt tccacatacg gttttgcgag aacgcttggg tggtgcgtac gccgccgggc
    20161 gcggctttga agcgttcgat ggcgcgggtg ttttcagggt tggttacgtc ccatttttca
    20221 atcgcgtctt tcagcgtcgg tgcgtggata gtgtgcacgt cggtgtgcag cttgcccgct
    20281 ttgtccaatt ctttcaggat ggcgaaaatg ccgccggctc ggtgcacgtc ttccatatag
    20341 tagtcgtggt tgttgggtgc ggttttgcag atgcagggca caacgcggct taagcggtcg
    20401 atgtctgcca ttttgaaatc cacgcccgct tcgttggcaa cggcgagtaa atgcaaaata
    20461 gtgttggtgc tgccgcccat cgcaatatcc atcgtcatag cgttttcaaa cgcttttttg
    20521 gtggcaatgc tgcgcggtaa cacggtttca tcgttttgct cgtaatagcg tttggtgatt
    20581 tcgacaatca tacggccggc ttcgaggaac aattctttgc ggccggcgtg ggtcgccaaa
    20641 tacgaaccgt tgccgggtag ggacagaccg agtgcttcgg tcaggcagtt catcgagttt
    20701 gccgtaaaca tacccgaaca cgagccgcag gtcgggcagg cgttttgttc gacttcttca
    20761 acttgccggt tgctgacatt gtcgtccgcc gattcaatca tcgcgtcaat caagtccaaa
    20821 cggcgttcgg gctggatgtt tgccacgccg ataactttac ctgcttccat cggaccgccg
    20881 gaaacgaaga tggtcgggat gttcaggcgc atcgcggcaa tcagcatgcc cggggtgatt
    20941 ttgtcgcagt tggaaatgca caccagcgcg tcggcgcagt gggcattgac catatattcg
    21001 atggagtcgg caatcaaatc gcggctgggc agggagtaca gcatgccgct gtgtcccata
    21061 gcgatgccgt cgtcgatggc gatggtgttg aattctttgg cgatggcgcc ggctttttcg
    21121 atttcgcggg caaccagctg gcccatattg tgcaggtgga catggccggg cacgaattgg
    21181 gtgaaggagt tggcaacggc gatgatgggc ttgccgaagt cggtttccat cacgccggtg
    21241 gcgcgccaca atgcacgtgc gcccgccata ttgcggccgt gggtggaggt tttggagcgg
    21301 tattctggca tagtgtgttt ccttgtgcct ataccgtctg aaagacaggg ctgtttcaga
    21361 cggtatcggg tacggttttt tagagtggga aaagagggta ttttatacca agtatcggaa
    21421 ttttgcggga ttgaaacggc gtgcggcaaa aaagaaaatc cccgcaggaa tgcggggacg
    21481 ggttcaggcg cgggcaatcg cgacggcttt ggatgcgtcc caaaaatcaa cgggtgcatt
    21541 taatacgggt ttgacgatgc ccgtccgtat ggcgaaatcg ccgaaacctt cgccgatatt
    21601 gcgttctgcc gcccatttgc cgatcaggtc gtccaattcg gcaaggattt cgggcagggt
    21661 gatgttttct ttataaaggc gggggatgcg tacgccttca cggtcgccgc cgatatggag
    21721 gttgtagcgt tcgacggctt tgccgaccag cccgatttcc gccaacattg cccgtccgca
    21781 gccgttgggg cagccggtaa tgcgggtaac gatgtagtcg tccgacgtgc cgtgtttcgc
    21841 cataatctta tccagctcgt cgatgaagtc cggcagcacg cgttcggctt ccgccattgc
    21901 cagcgggcag gtcgggaagg aaacgcacga catcgcattt tcgcgcagtt tgctgacatc
    21961 gttgcggatt aatccgtatg ttcgggcaag ttcttcgatt tttgctttgt ctgcttcggc
    22021 gacatttgcc acgatgaggt tttggttggc ggtaatgcgg aaatcgcctt tgtggatttt
    22081 ggcgatttcc aacacgccgg tcagaagctg tttcccgcct tcgtcaacca aacggccgct
    22141 ttcgatgaaa agggtcaaat gccagttgcc gtctatgcct tttacccagc cgatgcggtc
    22201 gccgcgcccg gtaaatttga acgggcgtat gggttcgaac ggcataccca tacggcgttc
    22261 gacttcggcg cggaagttgt ccaagcccat attttgaatg gtgtagcggg tgcgggcgtt
    22321 tttgcggtcg ctgcggttgc cgaagtcgcg ctgtgtggtt accaccgctt cggcggcctt
    22381 cagcgcgtgt tccggaggta cgaaacccag ttccagtgaa atgttcggat aggttttggt
    22441 gttgccgtgt tccattgaaa gcccgccgcc tgccaaaaca ttaaaaccgg caagctgtcc
    22501 gccgccgtct gaaatggcga caaaatccaa gtcgttgccg tagcagtcca catcatttaa
    22561 gggcgggatg acgactgcgg ttttgaattt gcgcggcaga taggttttgc ccaaaatcgg
    22621 ctcgtcttct tgaaggaagt cgtcggaact ttgaactttt ttgccgtcca cccacacatc
    22681 cagataaccg cgcgtgcgcg gcagcaggtg ttcggaaatc tttttcgcgt attcgtaagc
    22741 ctgccggtgc agttcggact cgatcgggtt ggacgtgcaa agcacgttgc ggttcatatc
    22801 cgccgccgtg gcgatggaat ccaaacccag tttgtgcaag aggcggtgca tcgtctgcaa
    22861 cttggctttc ggcacgccgt gaaattggaa ggtttgccgg ttggtcagcc ggatggagcg
    22921 gtaatgactg ttttcccggg caaatttgtc cagttctatc cattgggacg gtttgatgat
    22981 cccgcccggc agtcggcagc gcaaaagcat aaatttcaaa ggctcgagtt ttgcctcggc
    23041 gcgttcggcg cggatgtcgc ggtcgtcctg ctcatacata ccgtggaagc ggatgagttg
    23101 gaagttgtcg cctttgaagc cgcccgtgag cgggtctttc aaatcgtcca aaatcgtgcc
    23161 gcgtaaaaaa ttgctttcgg ttttcagacg ttcgttgtcg gatagcggtt tttcttgcca
    23221 cgccaaacct tttgtcttgg tctgtacggt cattttgtgt tcctcccgat tatatttaat
    23281 caataaacat cacgctgata gcgtttttct tcgcgcagca tatccaaata ttcttctgcg
    23341 ccctcttcgt ccaaatgtcc tgccccgata atcacatcca gcaaggcggc ttccacgtct
    23401 tttgccattt ttgccgcatc gccgcacaca tagatatgcg cgccttcctg cagccattgc
    23461 caaagtcctt ccgcctgttc gcggattttg tcctgcacat agattttttc ttcctgatcg
    23521 cgcgaccagg cgaaatcgta tctgtgcagg aagccgtctt tggcaaactg ctgccattcg
    23581 gtctgataga ggaagtcggc ggcaaaatgc ggattgccga aaatcagcca gtttttgcct
    23641 tccgcatttt ctgcggcacg ttgttggacg aaggcgcgga acggtgcgac gccggtgccc
    23701 gagccgatca tcacaatcgg cttgcggctg tcttcgggca gcctgaagcc gtcgttgcgt
    23761 tccacaaaca cgcgcaccgt gccgtcctct tccagccggt cggcaaggaa acccgatgcg
    23821 ccgcccgccc tggcgcgccc ttcgtgttca aaacgcaccg cgccgacggt caggtgcact
    23881 tcgtccccca cttccgcctg cgacgaggaa atcgaataca ggcgcggcgc aagtgggcgc
    23941 agcaggccgg cgaattgttc cgccgtcagt tttgccggga agcggtgcag cacatcggca
    24001 atcggcgtgc tttgcacaaa gccttgcaaa acggcgttgt cggcagcaat acggtcgagt
    24061 tcgtcatcat cggcgaacgg ggcatagcct ttgacaaagg cgggggtgtt ttgcgtgagt
    24121 tcaaaatggg ataacagtgc ggaggcaacc ggcagggttt ttccgcccgc ctgtatttcc
    24181 gttgccggat cgatgccgag caggtctagg atttccctga ccagtgccgg atcgttgtca
    24241 aaccaaacgc cgagcgcgtc gcccgggagg tagtgcaaat ccgaaccgct caaatcgatt
    24301 tcgatgtggc gcacgtcttt atcggattgg cgggcggtga ttttctgatt ggccagcagg
    24361 gcggcgggaa aggggtctgc cttgcagtac ctgccctccg atgccgtctg aaggccggcg
    24421 gggggcgttg cctgcggcgc gggcgttgcc cggttttttg cggcttcttc ttttaagagt
    24481 gcggcgatat tgtctgtcca ggcgtttgcg gcggcggcaa agtccaaatc cgcatcaacg
    24541 cgttcgagca ggcgtttcgc ccccaattct tcaaaacgtt tgtcgaaatc tttgcccgcc
    24601 cggcagaaat tcggatagga actgtcgccc aaacccagta cggcaaattg gagtttgtcc
    24661 aatttcgggg cttttttgcc gttcagcagt ttgtgcagca cgacggcttc ttccggcggt
    24721 tcgccttcgc cttgggtgga cgtaaccagc agcaggcggc gttcgccggc gatgtttttc
    24781 gccttatagt ctttcagttc ggcgcgactg acttggatgc cggcggcttc caggctgtcc
    24841 gccgctttgt cggcaacgga tttcgcattg ccggtttgcg aggcggaaag gacggttacg
    24901 gaaaaaggtt ctgccgtcgg caatgccgtc tgaagcgcgg gcagtcctgc agatgccccg
    24961 tttcctgctt ttgcccaagc gtagccggac agccacgccc attgtgccgc gtccagcccc
    25021 gacaggagct gcgtgatttc gggcggcaga ggcggtaatg gcggatttgt gttctgcata
    25081 tcgtgttcac tcataaaatc atacctgccg caacagtgcc gtatgtcgct tcgtctatca
    25141 ggataaacga accggcggcg gtattttccg cataaggcgt tgccgtaacg ggtttttgaa
    25201 ggttgatgcg gactttggcg atgtcgttca tcttcaagga ttccgcgccg gcctcttgtt
    25261 ccagcgtgcg gacatccaaa acgctttcaa tttccccgac ttttgccggc acggtttgcg
    25321 tgccgtgctt gagcaggtat ttgcgcgcgg tgttgagcgg acgttcgtca aaccagcaaa
    25381 gcgtggcttc cagatgtttt tgcggggcga gcggggaatt tttatcgaca aaaaggtcgc
    25441 cgcgcgaaac atcgatgtcg cggtccagcc ggagggttgc cgcctcgccg gcaaaagcct
    25501 gcgccacttc ccctttcggc gtgatgattt cggacacttc ggcggtcagc ccgttcggtt
    25561 cgatgcggac ggtttgcccg acggcgaccg aaccgcgttc gatgcgcccc tgatagcctc
    25621 ggaaatcatc ggccttgtcg gcatcttggc ggacgaccag ttgcacgggg aaataaaaat
    25681 cgtcggcggt gcggctgact tcgtccgccc ccggcagggt ttccaaaatg gacaataagg
    25741 gttcgccttt ataccaaggc atattgccgc cggggtaaac aatgttgtcg cccaagagtg
    25801 cggacaccgg tacgaaatgc acgtctttca aaccgagccg tccggcgagt cggcggtatg
    25861 cctccacaat ggcgttgaat ttgtcttcgc tgtaatccag caggtccatt ttgttgaccg
    25921 ccaccacaat atgcgggcag tcgagttggc ggaggatggc ggaatggcgt ttggtctgcg
    25981 gcagaagctg caagggctgt gcgccgaaat ccagttggga tgcgtcaacc agcacgactg
    26041 ccgccgaagc ggtgcttgcg cccgtaacca tattgcgcgt gtattgttcg tgccccggcg
    26101 tgtcggcgat gatgaatttc cgtttcgccg tggaaaaata gcggtatgcc acatcgatcg
    26161 taatgccctg ttcgcgttcg gcttccagtc cgtcggtcag gacggagaag tctatggttt
    26221 ctttcaaacc tttgcttttg ccggattcca aggttttgat ttggtcggac agcagggctt
    26281 tgctgtcgta gagcagccgt ccgattaggg tgcttttgcc gtcatcgacg ctgccggcgg
    26341 taatgaagcg gagcggggtc tggtgttgtg ccgtcatatt ttcttcctca tatctgctta
    26401 aagggttttt gaaatttaga aatagccttc ttttttgcgg ttttccattg ccgcctcgct
    26461 tgcctgatcg tccagccggg tcgcgctgcg ttcggaaatg tcggcaaccg ctgtttctct
    26521 gataatctcc gtcggcgtgg acgcggtgct ttctaccggg caggtgcagc tgatgtcgcc
    26581 gacggtgcgg aagcggacat caaggatttc ggaggtttca gacggcattt tcggggtgag
    26641 cggcgttacg gggaccagca gccccctgcg tctgaccact tcgcgcctgt ggctgtaata
    26701 aatcggcggc agctcgaggt tttcgcgggc gatgtattgc cagatgtcga gttccgtcca
    26761 gttggaaatc gggaagacgc gcatattttc gcctttgtgc agcctggtgt tgtacagcga
    26821 ccacagctcg gggcgttgcg ctttcggatc ccattgtccg aactcgtctc ggaacgagaa
    26881 aatccgttct ttggcgcggg ctttttcttc gtcgcgccgc gcgccgccca tcagtgcgtc
    26941 aaagccgttt gcctcgatgg tttccaacaa ggtaaccgcc tgtgccgcat tgcgcgaatc
    27001 ggtttctttg cgtaagacca ccgtgccttt ggcaatggag tcttccacgc gccccactat
    27061 caggcgggca ttgagttttg ccgcctgcgc gtcgcggaag gcaatcactt cggggtagtt
    27121 gtgccccgtg tcgatatgca ccagcgggaa gggcagtttc accggccggc tgcccagccg
    27181 gaaggctttg caggcgaggg cgagcaggac cacggaatct ttgccgccgg aaaagagcag
    27241 ggcggggttt tcgcattccg ccgccacttc gcggatgatg tggatggatt cggattccaa
    27301 ccagtcgagt tgggcgttgt tcggttcggt tttcgtcata cgatattcct tatttcttct
    27361 gtttgatcct tatggattat ttgtgcagcc cgcattcttt gctgtttttg tcttcccacc
    27421 accacctccc ggcgcgaatg tcttcgcccg ccttgacggg gcgggtgcag ggatcgcagc
    27481 ctatgctggg aaacccttgc cggtacaaat cgttgtaagg cacattgttg gcgaggatgt
    27541 atgcccacac gtcgtgttcc gaccagtcga aaatcgggtt gtatttgccg atgccccgtc
    27601 cggcatcata ttcggcaaac ggcagttccg tgcgtgtggc ggattgttcg cggcgttgcc
    27661 cggtaagcca ggcgtccgcg cctgcaatgg cgcggttgag cggttcggtt tttcggatgc
    27721 ggcagcattc gcggcgcgct tcaacgctgt cgtaaaaggc aaacctgcct ttgctttcca
    27781 cataatgcag ggcatcttcc cgtatcggtt ggaaacgctt gatttgcata tgcggataaa
    27841 cacgttcaat cctatccaac aggtttaaag tttccgcgtg cagcagcccc gtatccaaag
    27901 taaaaatacc gatattgagg ttttctccgg cgataaggtc ggtaattacc atatcttccg
    27961 cagcaaggct gctggcaaac cgtgcatccc ggtggctgcc ggcaatccgg tgcaggcgtt
    28021 gtttgagggt ttccgttttt tccgccaaag ctgtttcact gccattttca atcggcggaa
    28081 ttttccataa agcaggtttg aaaattttca ttttgtccgc cttgctttat gcggcttgtt
    28141 gttcttgtcc ggtgcccagt ccgtccaatg caaaatacgc gtgttcggcc ggattgtttt
    28201 cttttttggc gttttcgccg aaccaagcca gtttttcgtg cagttccacc acttcgccta
    28261 caacaattaa agccggcttc ggcgcgtttt cggcgaggcg ggcgaggttg gcgagcgtgc
    28321 cgattgccgt tgattgcgtt gattgcgtgc cgtggctgat gacggcggca ggcgtgtcgg
    28381 gcgggcggcc gtattgttgc agccgttcgg caatcagcgc ggctttgagc gtgcccatat
    28441 aaatcaccaa ggtttggcgg ctgcgggcaa gcgtctgcca ttcgatatcg ggcgcatccg
    28501 ccttgcggtg gccggtaacg aaaaccgcac tttgggcata atcgcggtgc gtgagcggga
    28561 tgccggcata ggcggtcgcg ccgacggcgg cggtaatgcc ggggacgacc gaaaacggaa
    28621 tgtggtggcg cgccagcact tccaattctt cgccgccgcg tccgaacacg aaagggtctc
    28681 cgcctttcaa acgcaccacg cgcctgcctt cgcgggccag cctgaccata agcgcattgg
    28741 tgtcctcttg cggggtgcgg tcgccccggg cgcgcttgcc gacaaaaatc cgttccgcat
    28801 cgcggcggac gagggacagt atgccgtctg aaaccagcgc gtcgtaaagc accacgtctg
    28861 cctgctggat ttcctgcagc cctttgagcg tcagcagccc cgcatcgccg ggacccgcgc
    28921 cgaccagcga gacggagccg ccttgatcat tttgacgact ttgttccaat tggcctgcca
    28981 attcccgttc ggcaagggtg ttttgccggt ttttgacgag ggctgcgaaa cgtccgttaa
    29041 actgcttttc ccaaaagcgg cggcgttcgg taacggattt cagtttgccc ttgaccgcat
    29101 cgcgccatct tccggagatt tccgccatat cgcccaaaga cggcggcagc agggcttcca
    29161 gcctttcacg cagcaggcgg gcgaggacgg gcgcgctgcc ggagctggaa acggcaatct
    29221 gaaccgggtt gcggtcgata accgacggga agatgaagct gcaatggtcg cggtcgtcca
    29281 ccacgttgac cggcttttgg cagctttcgg caagatggaa aacgcgccgg ttgagggctt
    29341 ggtcgctgct tgccgcaatg atgaggaaaa ctgtgcggat gtgttcggca cgaaattctt
    29401 cggcaagcca caggattttg ttttccgccg ccaacgcgga gagttcggca ttcaggtgtt
    29461 ttgcggcaac cctgacctct gcgcccgcct tcagcagcag gctgattttg cgtgcggcga
    29521 ccgcgccgcc gcctacgacc aatacggggc ggccggcgag gttggcgaaa atagggaaat
    29581 aattcactgg ctgactcctt tgctgtttgc ccgcaccttg tttccgatac ggtgcgtcgc
    29641 ggcatttttg tcggaatgcg ggtcatttta gacaaaagga ttttcccggt taaataataa
    29701 aaaggcattt gttagaagct gaaagctata tgagggcggc tgcggatacg gcggttttcc
    29761 gttttatagt ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagagaac
    29821 aattctctaa ggtgctgaag cactaagtaa atcggttctg tactatccgt actgtctgcg
    29881 gcttcgtctc cttgtcctga tttttgttaa tccactataa aacggtttgg gacggaaaaa
    29941 cggcctgaag ccgtttcggg cattcagacc gtttgcgcgg agaggggatg ccgtccgaag
    30001 ggcgaaaagg gcttcagacg gcattgatgt cgggtttcag gacaggagca ggatggcggc
    30061 tgcggcaagc gcggcaaccg ataatgcggc ggcaagcgcg gttttaccgg caaagcggat
    30121 tgaggttttg ccttcgatgt atttgaagcc ggttatcatc gggaggacga ggtttttctt
    30181 tttgaacacg cggtatgcgg cgacggcggc gatatgaatg gcggaaaaaa cggcgagcag
    30241 cttgaaaaag ttgaggtgga ttttccgcat aaggctgcct gtatgttcgg aaaccaaatg
    30301 gttgaggtag ccgttggtgc tgaaggtgtt ttcatcggcg gcaaaaagcc ccgtgccgac
    30361 ttgaaatgag acggcggcca aaagcgcgac gaccatcagt gcgcccaagg ggttgtgtcc
    30421 gggctggata tgttcgggaa taccgttttt cagataatcc ctgatacccg accatccgcg
    30481 gacgaaacgg gagaaacggg cggtatcgct gccccaaatg ccccagcaga ggcggaatac
    30541 gagcaggaaa aggataaaca gcccgacgcg cgtgtgccat tgcagcatat cgccgccggt
    30601 tttcgcgcta taccacataa agggtaggga tgcggcaagc agccaatgga aaaggcgggt
    30661 ggggaagtcc cagactttgg ttttgttttt cataatcggt ttccggcggt agaatcggtt
    30721 tgttttcgag ccttaatttt aaccgattgg aggggaaatg tttcccgttt tccatctttc
    30781 aggcgaaagc cgccgccgga tgcttcagac ggcattgcgt tttccctatg ttttcaaagc
    30841 ccgtgcggaa gattcgcaca aagggacttt cggcacgctc gccgtagtcg gcggatcggc
    30901 agggatgagc ggtgcgcccg tattggcggc atcggcggcg atgtatctcg gctgcggcaa
    30961 ggtgtgggcg ggtttcaatc aggatgcgct tccgtttgcc gtcattgccg gtttccccga
    31021 aattatgttg gacacggcag acggtttgac caagcgtcaa ggcataaacg cttggacggc
    31081 gggttgcggc ttgggtacgg ataaggcggc ggtcggaacg cttgccggaa ttttgacgga
    31141 acacacggac aagcccgtcc ttttggatgc ggacgcgctg aacatattat caaccgatgc
    31201 cgaaacccga aatctggcac gcgggtgtaa aaacctgatt ttaacgccac accacgccga
    31261 agccgcgcgc ctgcttggaa cgacggttgc acaggttcag gcggatcgga cggcggcagt
    31321 gaggaagata agggcaattt tcggcgcaac cgtggtttta aaggggcaca aaacattggt
    31381 tgcctcaccc gatacggaaa tctatgtcaa cgaaagcggc aacgcgggat tggcaacggc
    31441 gggcagtggc gacgtattgg gcggcatcat cggcagtctg ctcgcacagg gcgtgccggt
    31501 ttttgaagcc gcctgcgcgg gcgcgtggct gcacggcgcg gcggcggatg tcataaaaga
    31561 atcggcaggc attgcggcag ggctgttggc tggcgaaatc gccccggcgg caaggtggct
    31621 gcgcaaccgg ataactgaaa gtatgtaaga agatatagtg gattaacaaa aaccagtac
//
LOCUS       DAXHMX010000006        29386 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHMX010000006 DAXHMX010000000
VERSION     DAXHMX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.006"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            24..1106
                     /gene="mraY"
                     /locus_tag="ACFM4S_000199"
     CDS             24..1106
                     /gene="mraY"
                     /locus_tag="ACFM4S_000199"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6049962.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVAGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            1221..1493
                     /locus_tag="ACFM4S_000200"
     CDS             1221..1493
                     /locus_tag="ACFM4S_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6049963.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            1583..2920
                     /gene="murD"
                     /locus_tag="ACFM4S_000201"
     CDS             1583..2920
                     /gene="murD"
                     /locus_tag="ACFM4S_000201"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6049964.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            3057..4370
                     /locus_tag="ACFM4S_000202"
     CDS             3057..4370
                     /locus_tag="ACFM4S_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6049965.1"
                     /translation="MTGGFLKISEVLVKVGDGVHTLLLDRQIVRDGRKFDAPLLWMVV
                     LMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRL
                     VPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRRE
                     EVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLI
                     MVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAF
                     LDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFG
                     FFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGA
                     LPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            4374..5441
                     /gene="murG"
                     /locus_tag="ACFM4S_000203"
     CDS             4374..5441
                     /gene="murG"
                     /locus_tag="ACFM4S_000203"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6049966.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPAPAERFQGRKGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            5596..7005
                     /gene="murC"
                     /locus_tag="ACFM4S_000204"
     CDS             5596..7005
                     /gene="murC"
                     /locus_tag="ACFM4S_000204"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6049967.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            7118..8032
                     /locus_tag="ACFM4S_000205"
     CDS             7118..8032
                     /locus_tag="ACFM4S_000205"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6049968.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            8022..8750
                     /locus_tag="ACFM4S_000206"
     CDS             8022..8750
                     /locus_tag="ACFM4S_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6049969.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            8836..10080
                     /gene="ftsA"
                     /locus_tag="ACFM4S_000207"
     CDS             8836..10080
                     /gene="ftsA"
                     /locus_tag="ACFM4S_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6049970.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLQRWIENSF"
     gene            10199..11377
                     /gene="ftsZ"
                     /locus_tag="ACFM4S_000208"
     CDS             10199..11377
                     /gene="ftsZ"
                     /locus_tag="ACFM4S_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6049971.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            11515..12825
                     /locus_tag="ACFM4S_000209"
     CDS             11515..12825
                     /locus_tag="ACFM4S_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6049972.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            13450..14325
                     /gene="prpB"
                     /locus_tag="ACFM4S_000210"
     CDS             13450..14325
                     /gene="prpB"
                     /locus_tag="ACFM4S_000210"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6049973.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            14410..15564
                     /gene="prpC"
                     /locus_tag="ACFM4S_000211"
     CDS             14410..15564
                     /gene="prpC"
                     /locus_tag="ACFM4S_000211"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6049974.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEDR"
     gene            15976..16698
                     /locus_tag="ACFM4S_000212"
     CDS             15976..16698
                     /locus_tag="ACFM4S_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFC6049975.1"
                     /translation="MDKDKGSYAGIPVQNTKELVWQKIQEIPISECGEPLVSVGNTGR
                     IRVNPVYFSQGIEGAINTVLLRRSVADRLTQVVSLLPEMYGLEIFDGWRPVIVQRALR
                     ENMKQEIEKRHPEYDEVQVREALDCFAADPDRAGMPLPHLTGGSVDVGLFDIKSGKSL
                     DMGSEFDATGHLSYSDALEKEGEYFSSARNLRRILIHAMKEAGFSNLPTEWWHFDYGN
                     QNWAYFTQQSNAVFGAVEEQDT"
     gene            16724..17929
                     /locus_tag="ACFM4S_000213"
     CDS             16724..17929
                     /locus_tag="ACFM4S_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFC6049976.1"
                     /translation="MQTLISADNHLLLWAFVMLAAAAAIFIEQNSRLAGKVPGAVLAL
                     LIALIASNLGVIPTDAPVFDAVWSYIVPLAIPLLLFQLDLHALFKESGRMLFIFLISS
                     LGTVIGTLVSFALLKNYIPELDKVAGMISASYTGGGVNFAAMSAKLNPAKDVIAATIV
                     ADNLMMACYFFILIGISSSYWVRKVWGSPYQDKLEQEYHDPSVNKAAEYWKPKSISLQ
                     SIALSLAASILLVAVSFSLSSFLQNHFQSVDGVALKMLTGLISDKYLLLTTFTLAVVL
                     FFRKGIQKLDGSQELGTYCIYLFFVVIGVPASIPLIIKTAPLLFVFTLIIAVLNLLLT
                     LVLGKLFKFSIEEIVLACNANIGGPTTAAALAISKGWRDLVGAILLIGTVGYIIGNYI
                     GSFVYALLS"
     gene            18033..20639
                     /gene="acnD"
                     /locus_tag="ACFM4S_000214"
     CDS             18033..20639
                     /gene="acnD"
                     /locus_tag="ACFM4S_000214"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6049977.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQPGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAGKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGVVA"
     gene            20726..21172
                     /locus_tag="ACFM4S_000215"
     CDS             20726..21172
                     /locus_tag="ACFM4S_000215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6049978.1"
                     /translation="MKTQEDEVTKQKIFNHMINNKSFLNFQFWTFAALSAGSFAFLIS
                     AANIDSKDRDLTLEIAIVGYSVSLILNSILCFLIRGIEDDELSVKMLNEFNLDKRTFW
                     IPILATWSFALSTLFLLFTISLVGAITALIMAVLAIISFIKFTKKS"
     gene            21336..22505
                     /gene="prpF"
                     /locus_tag="ACFM4S_000216"
     CDS             21336..22505
                     /gene="prpF"
                     /locus_tag="ACFM4S_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6049979.1"
                     /translation="MPQIKIPAVYYRGGTSKGIFFKRTDLPEAAQQAGEARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            22919..24118
                     /locus_tag="ACFM4S_000217"
     CDS             22919..24118
                     /locus_tag="ACFM4S_000217"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6049980.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAVGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            24226..24846
                     /locus_tag="ACFM4S_000218"
     CDS             24226..24846
                     /locus_tag="ACFM4S_000218"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6049981.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(24973..25416)
                     /locus_tag="ACFM4S_000219"
     CDS             complement(24973..25416)
                     /locus_tag="ACFM4S_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6049982.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            25590..25958
                     /locus_tag="ACFM4S_000220"
     CDS             25590..25958
                     /locus_tag="ACFM4S_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6049983.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            26003..27157
                     /locus_tag="ACFM4S_000221"
     CDS             26003..27157
                     /locus_tag="ACFM4S_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6049984.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(27214..28086)
                     /locus_tag="ACFM4S_000222"
     CDS             complement(27214..28086)
                     /locus_tag="ACFM4S_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6049985.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATFPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            28171..28983
                     /locus_tag="ACFM4S_000223"
     CDS             28171..28983
                     /locus_tag="ACFM4S_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6049986.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKHWVLDAV"
BASE COUNT         6850 a         7386 c         8060 g         7090 t
ORIGIN      
        1 acaaaacaaa aaaaggaagc cccatgtttt tatggctcgc acatttcagc aactggttaa
       61 ccggtctgaa tatttttcaa tacaccacat tccgcgccgt catggcggcg ttgaccgcct
      121 tggcgttttc cctgatgttc ggcccgtgga cgatacgcag gctgaccgcg ctcaaatgcg
      181 ggcaggcagt gcgtaccgac ggcccgcaaa cccacctcgt caaaaacggc acgccgacga
      241 tgggcggttc gctgattctg accgccatta ccgtgtccac cctgttgtgg ggcaactggg
      301 cgaacccgta tatctggatt ctcttgggcg tattgctcgc taccggcgca ctcggttttt
      361 acgacgactg gcgcaaagtc gtctataaag accccaacgg cgtgtccgcc aaattcaaaa
      421 tggtgtggca gtcaagcgtt gccattatcg ccggtttggc attgttttac cttgccgcca
      481 attccgccaa caatattttg attgtcccgt tcttcaaaca aatcgccctg ccgctgggcg
      541 tggccggctt tttggtgttg tcttacctga ccatcgtcgg cacatccaac gccgtcaatc
      601 tcaccgacgg cttggacggc cttgccgcct tccccgtcgt cctcgttgcc gccggactcg
      661 ccattttcgc ctacgtcagc ggacactacc aattttccca atacctgcaa ttaccttacg
      721 ttgccggcgc aaacgaagtg gtgattttct gtaccgccat gtgcggcgcg tgcctcggtt
      781 tcttgtggtt taacgcctat cccgcgcaag tctttatggg cgatgtcggt gcattggcat
      841 tgggtgccgc gctcggtacc gtcgccgtca tcgtccgcca agagtttgtc ctcgtcatta
      901 tgggcggatt atttgtcgta gaagccgtat ccgttatgct tcaggttggc tggtataaga
      961 aaaccaaaaa acgcatcttc ctgatggcgc ccatccatca ccactacgaa caaaaaggct
     1021 ggaaagaaac ccaagtcgtc gtccgctttt ggattattac catcgtcttg gtgttgatcg
     1081 gtttgagtac cctcaaaatc cgctgaacct atgccgtctg aacacctttc agacggcatt
     1141 tgaacgcgca ataaacctgc ggcgacaatc cgcccagccc tatcgttaac ggcggcttat
     1201 actaaaacag aagtaaaacc atgaaacaga cagtcaaatg gcttgccgcc gccctgattg
     1261 ccttgggctt gaaccaagcg gtgtgggcgg atgacgtatc ggattttcgg gaaaacttgc
     1321 aggcggcagc acagggaaat gcagcagccc aatacaattt gggcgcaatg tattacaaag
     1381 gacgcggcgt gcgccaagac cttgtgctcg ctcaagaatg gtttggtaag gcttgtcaaa
     1441 acggagacca agacggctgc gacatttacc aacgcctgaa ggcaggttat tgaacagccc
     1501 gcgatgccgt ctgaaagcgg cttgggcagg ggcggacatc cccccacctg cccaatatga
     1561 tttgttttag gacaaaacca aaatgacgct tgaaaacaaa aaaatcctcg tcgccggact
     1621 cggcggtacg ggtatttcca tgattgccta tctgcgcaaa aacggcgcgg aggtggccgc
     1681 ttatgatgcg gagttgaagc ccgagcgcgt gtcgcaaatc ggtaagatgt ttgacgggct
     1741 ggtgttttac acaggccgtc tgaaagatgc gctggacaac ggtttcgata ttttggcact
     1801 cagccccggc atcagcgagc gtcagccgga tattgaggcg ttcaagcaaa acggcggacg
     1861 cgtgttgggc gacatcgaat tgctggcgga cattgtgaac cgccggggcg acaaggtaat
     1921 tgcgattacc ggcagcaacg gcaaaaccac ggtaacgagc ctggtcggct atctctgtat
     1981 caagtgcggg ctggataccg ttatcgcggg caatatcggc acgccggttt tggaggcgga
     2041 atggcagcgc gaaggcaaaa aggcggacgt gtgggtgttg gagctttcca gcttccaact
     2101 ggaaaacacc gaaagcctgc gcccgacggc ggcgacggta ctgaatattt ccgaagacca
     2161 tctcgaccgc tacgacgact tgctcgacta cgcgcatacc aaagccaaga ttttccgtgg
     2221 cgacggtgtg caggttttga atgcggacga tgcgttctgc cgtgccatga aacgtgccgg
     2281 gcgcgaggta aaatggtttt cgttggaaca cgaagccgat ttttggttgg aacgtgagac
     2341 aggccgcctg aaacaaggca atgaagattt gattgccacg caagatattc cgttgcaagg
     2401 cttgcacaac gccaccaacg tgatggctgc cgttgccttg tgcgaagctg tcggtttgcc
     2461 gcgcgaagca ttgctcgaac acgtcaaaac ctttcaaggc ctgccgcacc gcgtggaaaa
     2521 aatcggcgag aaaaatggcg tggtgtttat cgatgacagc aaaggcacga acgtcggtgc
     2581 gaccgccgcc gcgattgccg gtttgcaaaa tccgctcttc gtgattttag gcggcatggg
     2641 caaagggcag gacttcacgc ctctgcgcga cgcgcttgcc ggcaaggcaa aaggcgtgtt
     2701 cctgatcggc gttgatgcgc cgcaaatccg ccgcgatttg gacggctgcg gtctgaatat
     2761 gaccgactgc gccactttgg aagaagccgt tcagacggca tatgcccaag ccgaagcgtg
     2821 cgatatcgtg ctgctcagcc cggcttgtgc gagttttgat atgtttaaag gctacgcgca
     2881 ccgttcggaa gtgtttatcg aagcgtttaa ggctttgtga tgccgtctga aatgcaaacg
     2941 ccgtcattgt tgggcggcaa gtaaagattt agaataccga tttgggatgt atcgtatgtt
     3001 cggacggcat tgtctgccgt ctgaaatttt tgccctttgc ggcaggtgca aacagaatga
     3061 caggtggttt tttgaagatt tcggaagtat tggtaaaagt gggcgacggt gtccacactc
     3121 tgctgctcga caggcagatt gtgcgcgacg gcaggaaatt cgacgcgccg cttttgtgga
     3181 tggtggtgct gatgacggcg ttcagcctgc tgatgattta ttcggcttct gtgtatttgg
     3241 catcaaaaga aggcggcgat cagtttttct atttgaccag acaggcgggg ttcgtcgttg
     3301 ccggcttgat agcgagcggt tttttatggt ttctttgcag gatgaggaca tggcggcggc
     3361 ttgtgccgtg gatttttgcc ttatccggcc tgttgctggt agtcgtattg attgccgggc
     3421 gcgaaatcaa tggcgcgacc cgttggatac ctttgggtcc gttgaatttc cagccgaccg
     3481 agctgttcaa gctggcggtc atcctttatt tggcaagcct gttcacgcgc cgtgaagaag
     3541 tgttgcgcag catggaaagt ttgggttggc agtcgatttg gcgggggacg gccaatctga
     3601 tcatgtccgc caccaatccg caggcacgtc gtgaaacatt agaaatgtac ggccgtttcc
     3661 gggcgatcat cctgccgatt atgctggtgg cgttcggttt ggtgctgata atggtacagc
     3721 cggatttcgg ttcgtttgtc gtcattaccg tcattgccgt tggaatgctg tttttggcag
     3781 gattgccgtg gaaatatttt ttcgtcctgg taggcagcgt cttgggcggg atggtgctga
     3841 tgattaccgc cgctccctac cgtgtgcagc gggtagtggc atttttggac ccgtggaaag
     3901 acccgcaggg tgccggctac cagcttaccc actctctgat ggcaatcggg cgcggagagt
     3961 ggttcggtat gggtttgggt gcgagtttga gcaaacgcgg ctttctgccg gaagcgcata
     4021 ccgattttat ttttgccatc atcgccgaag aattcggttt cttcggtatg tgcgtgctga
     4081 tattctgtta cggctggctg gtggtgcggg cgttttccat cggcaagcag tcgcgcgatt
     4141 tgggtttgac tttcaacgcc tatatcgctt cgggtatcgg catttggatc ggtatccaaa
     4201 gtttcttcaa tatcggtgtg aacatcggtg ctttgccgac caaaggtctg acgctgccgt
     4261 tgatgtccta tggcggttcg tcagtctttt tcatgctgat cagcatgatg ctgctgttgc
     4321 gtatagatta tgaaaaccgc cggaaaatgc gcggttatcg ggtggagtaa atcatgggcg
     4381 gtaaaacctt tatgctgatg gcgggcggaa cgggcggaca tattttcccc gcgctggcgg
     4441 tggcggattc attgcgcgcg cgcggccatc atgtgatttg gctgggcagc aaggattcga
     4501 tggaagagcg tatcgtgccg caatacggca tacgcttgga aacgctggcg attaaaggcg
     4561 tgcgcggcaa cggcatcaaa cgcaagctga tgctgccgtt tactttgtat caaaccgtcc
     4621 gcgaagcgca gcggattatc cgcaaacacc gtgtcgagtg cgtcatcggc ttcggcggct
     4681 tcgttacctt ccccggcggt ttggcggcga agctattagg cgtgccgatt gtgattcacg
     4741 agcaaaacgc cgtggcaggt ttgtccaacc gccacctgtc gcgctgggcg aagcgggtgt
     4801 tgtacgcttt tccgaaagcg ttcagccacg aaggcggctt ggtcggcaac cccgtccgcg
     4861 ccgatattag caacctgccc gcgcctgccg aacgcttcca agggcgtaaa ggccgtctga
     4921 aaattttggt ggtcggcggc agtttgggcg cggacgtttt gaacaaaacc gtaccgcagg
     4981 cattggcttt gctgcccgac aatgcgcgtc cgcagatgta ccaccaatcg ggacggggca
     5041 agctgggcag cttgcaggcg gattacgacg cgctgggcgt gaaagccgaa tgcgtggaat
     5101 ttattaccga tatggtgtcc gcctaccgcg atgccgattt ggtgatttgc cgtgccggcg
     5161 cgctgacgat tgccgagttg acggcggcgg gattgggtgc gttgttagtg ccgtatcctc
     5221 acgccgttga tgaccatcaa accgccaacg cgcgttttat ggtgcaggca gaagcaggac
     5281 tgctgttgcc gcaaacccag ttgacggcgg aaaaactcgc cgaaatcctc ggcagcctga
     5341 accgcgaaaa atgtctcaaa tgggcggaaa acgcccgtac tttggcattg ccgcacagcg
     5401 cggacgacgt tgccgaagcc gcgattgcgt gtgcggcgta aactgccgaa ccatgccgtc
     5461 tgaaatgccg tccagacagc atggatgttt tttatttcaa tccgctatat atttgtcaga
     5521 aaactatggc gcgcaaacgg tcagcccttt aaaataacgc ctttacgcat cgaaaatcca
     5581 ccggaacgca acattatgat gaaaaatcga gtgaccaaca tccattttgt cggtatcggc
     5641 ggcgtcggca tgagcggcat cgccgaagtc ttgcacaatt tgggttttaa agtttccggt
     5701 tcggatcagg cgcgtaatgc cgctaccgag catttgggca gcctgggcat tcaagtttat
     5761 cccggccata ccgcagaaca cgttaacggt gcggatgtcg tcgttacctc taccgccgtc
     5821 aaaaaagaaa atcccgaagt tgtcgctgcg ttggagcagc aaattcccgt tattccgcgc
     5881 gccctgatgt tggcggagtt gatgcgcttc cgtgacggca tcgccattgc cggcacgcac
     5941 ggcaaaacca cgaccaccag cctgaccgcc tccatcctcg gcgcggcagg acttgacccg
     6001 actttcgtta tcggcggcaa actcaacgcc gcaggcacca acgcccgctt gggcaaaggc
     6061 gaatacatcg ttgccgaagc cgacgagtcg gatgcatcct ttctgcacct gacaccgatt
     6121 atgtccgtcg ttaccaatat cgacgaagac catatggata cctacgggca cagcgtcgaa
     6181 aaactgcatc aggcgtttat cgatttcatc caccgtatgc ccttctacgg caaagccttt
     6241 ttgtgtattg acagcgaaca cgtccgcgcg attttgccca aagtgagcaa accttatgct
     6301 acttacggtt tggacgatac cgccgacatc tacgccaccg acatcgaaaa cgtcggcgcg
     6361 caaatgaaat tcaccgtcca tgttcaaatg aaaggacatg agcaggggtc gtttgaagtc
     6421 gtgctgaata tgcccggcag acacaacgtg ctgaacgcat tggcagccat cggcgtggcg
     6481 ctggaagtcg gcgcatcggt tgaagcgatc caaaaaggct tgctcggctt tgaaggtgtc
     6541 ggccgccgct tccaaaaata cggcgacatc aagttgccaa acggcgggac cgcgctcttg
     6601 gtggacgact acggacacca ccccgtcgaa atggcggcga cccttgccgc cgcacgcggc
     6661 gcgtatccgg aaaaacgttt ggtactcgcc ttccagccgc accgctatac ccgcacgcgc
     6721 gatttgtttg aagactttac caaagtcctc aataccgttg acgcgctggt gctgaccgaa
     6781 gtttatgccg ccggtgaaga gccgattgcc gccgccgatt cccgcgctct tgcccgcgcc
     6841 atccgcgtgt tgggcaaact cgagccgatt tactgcgaaa acgttgccga tctgcccgaa
     6901 atgctgttga acgttttgca ggacggcgac atcgtgttga atatgggtgc gggaagcatc
     6961 aaccgcgtcc ccgccgcgct gctggaattg tcgaaacaga tttgaggcac acccgcctga
     7021 cagacggaac atcatataaa ggtcgtctga aaccgcaaat caggtttcag acgacctctg
     7081 gcaacaagca taaagcaatc aggaaagaac aaaaacaatg cagaattttg gcaaagtggc
     7141 cgtattgatg ggcggttttt ccagcgaacg agaaatctcg ctggacagcg gcaccgccat
     7201 tttgaatgct ttaaaaagca aaggcataga cgcatatgcc ttcgatccta aagaaacccc
     7261 attgtctgaa ttgaaggcac aaggttttca gacggcattc aacatccttc acggtactta
     7321 cggcgaagac ggggcggttc agggtgcatt ggaactgttg ggcattccct ataccggcag
     7381 cggtgtcgcc gcatccgcca tcggcatgga caaataccgc tgcaaactga tttggcaggc
     7441 attgggactg cccgttcccg agttcgccgt cctgcacgac gacactgatt tcgatgccgt
     7501 cgaagaaaaa ttgggcctgc cgatgtttgt gaaaccggcg gccgaaggca gcagcgtagg
     7561 cgtggtaaaa gtcaaaggaa aaggccgtct gaaaagcgtt tacgaagaat tgaaacacct
     7621 tcagggcgaa atcattgccg aacgttttat cggcggcggc gaatattcct gccccgtcct
     7681 gaacggcaaa gggctgcccg gcatacacat cattcccgca accgagtttt acgactacga
     7741 agccaagtac aaccgcgacg acaccattta tcaatgtcct tcggaagatt tgaccgaagc
     7801 cgaagaaagc ctgatgcgcg aactggcggt tcgcggcgcg caggcaatcg gtgcggaagg
     7861 ctgcgtgcgc gtcgatttcc tcaaagatac cgacggcaaa ctctatctgt tggaaatcaa
     7921 caccctgccc ggtatgacga gccatagttt agtaccgaaa tccgctgccg ttacgggcgt
     7981 gggttttgcc gatttatgta ttgaaatttt gaagaccgca catgtgggat aatgccgaag
     8041 cgatggaacg gctgacgcgc tggctgcttg tcatgatggc gatgctgctt gctgcgtccg
     8101 ggctggtttg gttttacaat tcgaatcatc tgcccgtcaa gcaggtgtcg ctgaagggca
     8161 acctggttta ttccgataag aaggcattgg gcagtttggc gaaagaatac atccatggga
     8221 atattttgag gacggacatc aatggcgcac aggaggccta ccgccggtat ccgtggattg
     8281 cgtcggtcat ggtgcgccgc cgttttcccg acacggttga ggtcgtcctg accgagcgca
     8341 agccggtcgc gcgttggggc gaccatgcct tggtggacgg cgaaggcaat gtttttgaag
     8401 cccgcttgga cagacccgga atgccggtat tcagaggcgc ggaaggaacg tctgccgaaa
     8461 tgctccgccg ttatgacgaa ttttcgactg ttttggcaaa acagggtttg ggcatcaaag
     8521 agatgaccta tacggcacgt tcggcgtgga ttgtcgtttt ggacaacggc atcaccgtca
     8581 ggctcggacg ggaaaacgag atgaaacgcc tccggctttt taccgaagcg tggcagcatc
     8641 tgttgcgtaa aaataaaaat cggttatcct atgtggatat gaggtataag gacggatttt
     8701 cagtccgcta tgcttccgac ggtttacccg aaaaagaatc cgaagaatag tgggaacagg
     8761 tatcggacag attacggccg tgccgtctga aacggtgcga cgcaaatttc aatcagtttt
     8821 aagagcagac gaacaatgga acagcagcaa agatacatca gcgtactgga tatcggtacg
     8881 tctaaagtcc tcgcactgat cggggaagtt caagatgacg acaaaatcaa catcgtcggt
     8941 ttggggcagg ctccttcacg gggcttgcgc gcgggcatgg taaccaatat cgatgccacc
     9001 gtccaagcca tcaggcaggc ggtcaatgat gccgagctga tggcggatac caaaattacc
     9061 cacgttacca caggtatcgc aggcaaccac atccgcagtc tcaattcgca aggtgtggtt
     9121 aaaattaaag acggggaagt cacgcaggca gacatcgacc gcgccattga aacggcaaag
     9181 gcaatcaata tcccgcccga tcaaaaaatt ctcgatgccg tggttcaaga ctacattatc
     9241 gacacccaac ttggcgtgag ggagcccatc ggtatgagcg gtgtgcgtct ggatacgcgg
     9301 gtgcacatca ttaccggtgc aagtacggca gtgcagaatg tccaaaaatg tatcgagcgg
     9361 tgcggtttga aaagcgatca gattatgctt cagccgttgg caagcgggca ggcggtgctg
     9421 actgaagatg aaaaagacct cggtgtatgc gtcatcgaca ttggtggcgg aacgaccgat
     9481 attgccgttt atatgaacgg cgccatccgc catacgtccg tcattccggc cggtggtaat
     9541 ctgattacca aagatttgtc caaatcgttg agaacacctc tcgatgccgc cgagtacatt
     9601 aaaatccatt atggcgtggc atcatgcgat acggaaggct tgggtgagat gattgaagtt
     9661 ccgggcgtgg gtgaccggac atcgcgtcag gtttccagta aggttttggc agcaatcatc
     9721 agtgcacgta ttcaggagat ttttggcgta gtgctgggcg agctgcaaaa atcgggtttc
     9781 cccaaagaag tgctgaatgc gggtatcgtt ctgaccggcg gtgtgtccat gatgaccggg
     9841 attgtggaat ttgccgaaaa aatcttcgat ttgcctgtac gcaccggtgc accccaagaa
     9901 atgggcggtt tgtccgaccg cgtccgcaca ccgcgttttt ctaccgctat cgggctgctt
     9961 catgcagcat gcaagctgga aggaaacttg ccgcagccgg aaaacggtgc agtgcaagag
    10021 agggaagggg gcggcggttt gttggcaaga ttgcaacggt ggattgaaaa cagcttctga
    10081 acaggtggat tgccgtttga caggtgagaa gtattttgcc agcagcaaga tacttcttat
    10141 ataatgaata ataatttatt taaaccgtcc tctgaatggg gcgagcagga gtttttgaat
    10201 ggaatttgtt tacgacgtgg cagaatcggc agtcagccct gcggtgatta aagtaatcgg
    10261 cttgggcggc ggcggttgca atgcaatcaa taacatggtt gccaacaatg tgcgcggtgt
    10321 ggagtttatc agtgccaata cggatgcgca gtctctggca aaaaaccatg cggcgaagag
    10381 aatccagttg ggtacgaatc tgacacgcgg tttgggcgcg ggcgcgaatc ccgatatcgg
    10441 ccgtgcggca gcccaggaag accgggaagc cattgaagaa gccattcgcg gtgcgaatat
    10501 gctgtttatc acgaccggta tgggcggcgg taccggtacc ggttccgcgc cggttgttgc
    10561 tgagattgcc aagtctttgg gcattctgac cgttgccgtg gttacccgac cgttcgcata
    10621 tgaaggtaag cgcgtccatg tcgcacaggc agggttggaa cagttgaaag aacacgtcga
    10681 ttcgctgatc atcatcccga acgacaaact gatgactgca ttgggtgaag acgtaacgat
    10741 gcgcgaagcc ttccgtgccg ccgacaatgt attgcgcgat gcggtcgcag gcatttccga
    10801 agtggtaact tgcccgagcg aaatcatcaa cctcgacttt gccgacgtga aaaccgtgat
    10861 gagcaaccgc ggtatcgcta tgatgggttc gggttatgcc caaggtatcg accgtgcgcg
    10921 tatggcgacc gaccaggcca tttccagtcc gctgctggac gatgtaacct tggacggagc
    10981 gcgcggtgtg ctggtcaata ttacgactgc tccgggttgc ttgaaaatgt ccgagttgtc
    11041 cgaagtcatg aaaatcgtca accaaagcgc gcatcccgat ttggaatgca aattcggtgc
    11101 ggctgaagac gagaccatga gcgaagatgc catccggatt accattatcg ctaccggtct
    11161 gaaagaaaaa ggcgcggtcg attttgttcc ggcaagggag gtagaagcgg ttgccccgtc
    11221 caaacaggag caaagccaca atgtcgaagg tatgatccgc accaatcgcg gtatccgcac
    11281 gatgaacctt accgctgcgg atttcgacaa tcagtccgta cttgacgact ttgaaatccc
    11341 tgctattttg cgtcgtcaac acaattcaga caaataatgt gctgtttgcc cgtaaacctg
    11401 ctgcctcccg aatcggtttg tccggtttgg gaggtatgtt tttcaagatg ttgcaatttc
    11461 gtacggtttg cggtcggcgg attcagattt ttccacttga tacagacttc agatatggac
    11521 acttcaaaac aaacactgtt ggacgggatt tttaagctga aggcaaacgg tacgacggtg
    11581 cgtaccgagt tgatggcggg tttgacaact tttttgacga tgtgctacat cgttatcgtc
    11641 aaccctctga ttttgggcga gaccggcatg gatatggggg cggtattcgt cgctacctgt
    11701 atcgcgtctg ccatcggctg ttttgttatg ggttttgtcg gcaactatcc gattgcactc
    11761 gcaccgggga tggggctgaa tgcctatttc acctttgccg tcgttaaggg tatgggcgtg
    11821 ccttggcagg ttgcgttggg tgcggtgttc atctccggtc tgatttttat cctgttcagc
    11881 ttttttaaag tcagggaaat gctggtcaac gcactgccta tgggtttgaa aatgtcgatt
    11941 gctgccggta tcggtttgtt tttggcactg atttccctga aaggcgcagg cattatcgtt
    12001 gccaatccgg caaccttggt cggcttgggc gatattcatc agcccagcgc actgttggca
    12061 ttgttcggtt ttgtcatggt ggtcgtattg gggcatttcc gcgttcaagg cgcaatcatc
    12121 atcaccattc tgacgattac cgtcattgcc agcctgatgg gtttgaatga atttcacggc
    12181 atcatcggcg aagtaccgag cattgcgccg acttttatgc agatggattt tgaaggcctg
    12241 tttaccgtca gcatggtcag tgtgattttc gtcttcttct tggtcgatct atttgacagt
    12301 accggaacgc tggtcggcat atcccaccgt gccgggctgc tggtggacgg taagctgccc
    12361 cgcctgaaac gcgcactgct tgcagactct accgccattg tggcaggtgc ggctttgggt
    12421 acttcttcca ccacgcctta tgtggaaagc gcggcgggcg tatcggcagg cggacggacc
    12481 ggcctgacgg cggttaccgt cggcgtattg atgctcgcct gcctgatgtt ttcacctttg
    12541 gcgaaaagtg ttcccgcttt tgccaccgcg cccgccctgc tttatgtcgg cacgcagatg
    12601 ctccgcagtg cgagggatat tgattgggac gatatgacgg aagccgcacc cgcattcctg
    12661 accattgtct tcatgccgtt tacctattcg attgcagacg gcatcgcctt cggcttcatc
    12721 agctatgccg tggttaaact tttatgccgc cgcaccaaag acgttccgcc tatggtatgg
    12781 attgttgccg tattgtgggc actgaaattc tggtatttgg gctgattgat tcgatattaa
    12841 aaatgccgtc tgaaaggttt tcagacggca ttttgtttgc cgatatattt aatttttatt
    12901 aaattatata aaaatcaaat agataataaa ctacatcaga ttacttaaaa ataatacatt
    12961 gtttttatgt ataaaatatt ttataaggtt tcaggatttt gattatcgaa aatttttctt
    13021 gatttcctga caattttatt gaaacaaata attcaaaatt aatctagttt aatcatggaa
    13081 ttaaaataaa atattaaaat tatgtaatga gtctccttaa aaatgtttga cattttcagt
    13141 cttgtgtttt agattagcga aaaataaaac tacacaatac tacaaaggaa cattactatg
    13201 aaaccaattc agatgttttc cccttttctg aataatcccc ttgttttctt cttgtctgcg
    13261 gttttgccgc ataattccga acggtctgct gtttttcttt gattcgtttt aaatatcaat
    13321 aagataattg ttctcatata tttttaatga ttggattggg atgcccgacg cgtcggatgg
    13381 ctgtgttttg ccgtccgaat gtgatggaag cctgtccata ctgaaaaaag tctataaagg
    13441 agaaatatga tgagtcaaca ctctgccgga gcacgtttcc gccaagccgt gaaagaatcg
    13501 aatccgcttg ccgtcgccgg ttgcgtcaat gcttattttg cacgattggc cacccaaagc
    13561 ggtttcaaag ccatctatct gtccggcggc ggcgtggcag cctgttcttg cggtatccct
    13621 gatttgggca ttaccacaat ggaagatgtg ctgatcgacg cacgacgcat tacggacaac
    13681 gtggatacgc ctctgctggt ggacatcgat gtgggttggg gcggtgcatt caatattgcc
    13741 cgtaccattc gcaactttga acgcgccggt gttgcagcgg ttcacatcga agatcaggta
    13801 gcgcaaaaac gctgcggcca ccgtccgaac aaagccattg tatctaaaga tgaaatggtc
    13861 gaccgtatca aagctgccgt agatgcgcgc gttgatgaga acttcgtgat tatggcgcgt
    13921 accgatgcgc tggcggtaga aggtttggat gccgctatcg aacgcgccca agcttgtgtc
    13981 gaagccggtg cggacatgat tttccctgaa gccatgaccg atttgaacat gtaccgccaa
    14041 tttgcagatg cggtgaaagt gcccgtgttg gcgaacatta ccgagtttgg ttccactccg
    14101 ctttataccc aaagcgagct ggctgaaaac ggcgtgtcgc tggtgctgta tccgctgtca
    14161 tcgttccgtg cagcaagcaa agccgctctg aatgtttacg aagcgattat gcgcgatggc
    14221 actcaggcgg cggtggtgga cagtatgcaa acccgtgccg agctgtacga gcatctgaac
    14281 tatcatgcct tcgagcaaaa actggataaa ttgtttcaaa aatgatttac cgctttcaga
    14341 cggccttcca atctatccgc atcggccgtc tgaaaattga aaatccataa aaaacaaagg
    14401 agaaatacga tgactgaaac tactcaaacc ccgactttca aacctaaaaa atccgttgcg
    14461 ctttccggcg ttgcggcggg taatacagca ttgtgtaccg tcggccgtac cggcaacgat
    14521 ttgagctatc gcggttacga catcttggat ttggcacaaa aatgcgagtt tgaagaagtc
    14581 gcccacctgc tgattcacgg tcatttgccc aacaaattcg agctggccgc ttataaagcc
    14641 aagctcaaat ccatgcgcgg cctgcctatc cgtgtgatta aagttttgga aagcctacct
    14701 gcacataccc atccgatgga tgtgatgcgt accggcgtat ccatgctggg ctgcgttcat
    14761 cctgaacgtg aaagccatcc ggaaagcgaa gcgcgcgaca tcgccgacaa actgatcgcc
    14821 agcctcggca gcatcctctt gtactggtat caatattcgc acaacggcaa acgcattgag
    14881 gttgaaagcg acgaagagac catcggcggt catttcctgc aactgttgca cggcaaacgc
    14941 ccaagcgaat cccacatcaa agccatgcac gtttcactga ttctgtatgc cgaacacgag
    15001 ttcaacgctt ctaccttcac cgcccgcgtg attgccggta caggctctga tatgtactcc
    15061 agcattaccg gagcaatcgg cgcgttgaaa ggtccgaaac acggcggcgc gaacgaagtg
    15121 gcttacgata ttcaaaaacg ctaccgcaat gccgacgaag ccgaagccga catccgcgaa
    15181 cgcatcggcc gcaaagaaat cgtgatcggt ttcggtcatc cggtgtacac catctccgac
    15241 cctcgcaacg ttgtcattaa agaagtggca cgcggtttga gcaaagaaac cggcgatatg
    15301 cgcctctttg acattgccga acgtttggaa agcgtgatgt gggaagagaa aaaaatgttc
    15361 ccgaatctgg actggttctc cgccgtttcc tatcaaaaat tgggcgtacc gaccgctatg
    15421 ttcacaccgc tgttcgtgat ttctcgtaca accggttgga gcgcacacgt tctcgagcaa
    15481 cgcaaagacg gcaaaatcat ccgtccgagc gcgaactaca cgggccctga agatttggcg
    15541 tttgtggaga ttgaagaccg ctaatacgcg taacagcagg gtgggccgcc agcccgcccc
    15601 atctcgaaat gccgtttgaa aacaatgaat aaatgatgtt ttcgtttttc gatggcctag
    15661 tgcagtatgc gagagagagc cggccgtttg gaaaacatgc tgctctgctg tcgtcattcc
    15721 catacaggca gaaattcaaa agttgaaatg ttctgctgta aagagtgctg aaagatttaa
    15781 gtttggattt ccgtctatac gggaatgatg gtagcggagc agcaattttt ttcataccgc
    15841 gaatagattt cagataacaa gcaataaaac agaatttatg gtttcagacg gcctgaaggt
    15901 ccgaatcaac aggccgtctg aaaaaagaaa tttaatgaat ttattggcat actaattatt
    15961 aatgcggaac atttgatgga taaggacaag gggtcttatg ctggtattcc cgtacaaaat
    16021 accaaagagt tagtttggca gaaaatacaa gaaataccta tttctgaatg tggggaaccg
    16081 ttggtcagtg ttggtaatac cggacgcatt cgtgtcaatc ctgtttattt ttcccagggt
    16141 attgaaggtg caataaatac tgttttattg cgtagaagtg ttgctgatag gctgactcag
    16201 gttgtctctt tgctgccaga aatgtacgga ttggaaattt ttgatggttg gcgtcctgtc
    16261 atagtgcaaa gagcattacg agaaaatatg aagcaagaaa ttgaaaaacg ccatccggaa
    16321 tatgatgaag ttcaagttag agaagcactg gactgttttg ctgctgatcc agatcgggca
    16381 ggaatgcctc tgccccattt gacaggcgga tctgttgatg tcggattgtt tgatataaaa
    16441 agtggcaaaa gtttggatat gggaagtgag tttgatgcaa ccggacattt atcttatagc
    16501 gatgctttag agaaagaagg tgagtatttc tcctctgctc gtaatttacg ccggattttg
    16561 attcatgcga tgaaagaggc aggatttagt aatttgccaa ccgaatggtg gcatttcgat
    16621 tatggcaacc aaaattgggc ttattttacc caacaaagca acgcagtttt tggggcggta
    16681 gaagagcagg atacttaaat taaaataaaa attgggagga aaaatgcaaa ctttaatttc
    16741 agcagataat catttgctgc tgtgggcttt tgttatgtta gctgccgctg ctgcgatttt
    16801 tattgagcaa aacagtcgct tggctgggaa agtccctggt gcagtattag ctttattgat
    16861 tgcgttgatt gcttctaatt tgggtgttat tccgacggat gctccagtat ttgatgctgt
    16921 atggtcttat attgtacctc ttgctattcc gttgttattg tttcaattag atcttcatgc
    16981 tctatttaag gaaagtggac gtatgctgtt tattttcctg attagttcac tagggacggt
    17041 cattggaacg cttgtctcat ttgcactatt gaaaaactat attcctgaac tagataaggt
    17101 agctggcatg atttcagcat cttatacggg aggtggtgtt aattttgcag ctatgtcagc
    17161 taagcttaac cctgctaaag atgttattgc agcaacaatt gttgctgata atttgatgat
    17221 ggcttgttac ttttttattt tgattggtat ctcttcctca tattgggtca gaaaagtttg
    17281 gggaagtcca tatcaggata aattggagca ggaatatcat gatccgagtg tgaataaggc
    17341 tgccgagtat tggaaaccta aatccatttc gctacagagt atagccttat ctttggctgc
    17401 ctcgattttg ttggtcgcag tttcattctc attatccagt tttttgcaaa atcattttca
    17461 aagtgttgat ggtgttgcat taaaaatgct gacaggttta attagtgaca aatatttgct
    17521 gttaacgaca tttacattgg cagttgtttt atttttccgt aaaggcattc aaaaactaga
    17581 tggcagtcaa gaattaggga catattgtat ttatctgttt tttgttgtga ttggtgttcc
    17641 tgcctctata ccgttaatta ttaaaacagc accattactc tttgttttta ctttgattat
    17701 tgcggtattg aatttgttac tgacgttagt gttaggcaag ctgttcaaat tcagtattga
    17761 agaaattgta ttggcttgta atgccaatat tggagggccg actactgcag cagccttagc
    17821 catcagtaaa ggttggcgtg atttagtcgg tgcaattttg ttaattggta ctgtaggcta
    17881 cattatcggt aactatatag gttcgtttgt atatgcccta ttgagctgaa gactaatcta
    17941 acaatccatg aaccttttca gacgaccccc ttcaaaaaac gtcgtctgaa accccaaacc
    18001 acaagaaaaa tagatccaca ggagaactga acatggctgc caaccaacgt taccgcaaac
    18061 cgctacccgg tacggatttg gaatactacg acgcgcgtgc ggcgtgtgag gacatcaaac
    18121 ccggctctta cgacaagctg ccttatacaa gccgtatctt ggctgagaac ttggtcaacc
    18181 gcgcagacaa agtcgacctg ccgacgctgc aaagctggct gggccagttg atagaaggga
    18241 agcaggaaat cgactttccg tggtatcccg cgcgggtggt gtgccacgat attctggggc
    18301 agaccgcgct ggtggatttg gcgggcttgc gcgatgcgat tgccgaaaaa ggcggcgatc
    18361 ctgccaaagt gaatccggtg gtgcaaaccc agcttatcgt cgaccactct ctggcggtgg
    18421 agtgcggcgg ttacgatcct gatgccttcc gcaaaaaccg cgaaatcgaa gaccggcgca
    18481 acgaagaccg tttccacttc atcaactgga caaaaaccgc atttgaaaat gtggacgtga
    18541 ttccggcggg caacggcatt atgcaccaaa tcaatctgga aaaaatgtcg cccgtcgtcc
    18601 aagtcaaaaa cggcgtggct ttccccgata cctgcgtcgg cacggattcg cacacgccac
    18661 acgtcgatgc gctgggcgtg atttccgtag gcgtgggcgg tttggaagcc gaaaccgtaa
    18721 tgttaggccg tgcatccatg atgcgtctgc ctgatattgt cggcgttgag ttgactggca
    18781 aacgtcaacc tggcattact gccaccgaca tcgtgttggc actgaccgaa ttcttgcgta
    18841 aagagcgcgt ggttggcgca tttgtcgaat tcttcggcga gggcgcaaga agcctgtcta
    18901 tcggcgaccg agccactatt tccaacatga cccctgagtt cggcgcgact gccgcgatgt
    18961 tcgctattga tgagcaaacc attgattatt tgaaactgac aggccgcgac gacgcgcaag
    19021 tgaaattggt ggaaacctac gccaaaaccg caggcttgtg ggcagatgcc ttgaaaaccg
    19081 ccgtttatcc tcgcgtattg aaatttgatt taagcagcgt aacgcgcaac atggcaggcc
    19141 caagtaaccc gcatgcccgt tttgcgactg ccgatttggc cggcaaaggc ttggctaaac
    19201 cttatgaaga gccttcagac ggccaaatgc ctgacggtgc agtgattatt gccgcgatta
    19261 cttcctgtac caatacttcc aatccgcgca acgttgtcgc cgccgcgctg ttggcacgca
    19321 atgccaaccg cctcggcttg caacgtaaac cttgggtgaa atcttcgttt gccccgggtt
    19381 caaaagtagc cgaaatctat ttgaaagaag caggcctact gcctgaaatg gaaaaactcg
    19441 gcttcggtat cgttgccttc gcatgtacca cctgtaacgg catgagcggt gcgctggatc
    19501 cgaaaatcca aaaagaaatc atcgaccgcg atttgtacgc caccgccgta ttatcaggca
    19561 accgcaactt cgacggccgt atccacccgt atgcgaaaca ggctttcctc gcttcgcctc
    19621 cgttggtcgt tgcctacgcg ctggcaggca gtatccgttt cgatattgaa aacgacgtac
    19681 tcggcgttgc agacggcaag gaaatccgcc tgaaagacat ttggcctgcc gatgaagaaa
    19741 tcgatgccgt cgttgccgaa tatgtgaaac cgcagcagtt ccgcgatgtg tatgtaccga
    19801 tgttcgacac cggcacagcg caaaaagcac ccagtccgct gtacgattgg cgtccgatgt
    19861 ccacctacat ccgccgtccg ccttactggg aaggcgcgct ggcaggggaa cgcacattaa
    19921 gaggtatgcg tccgctggcg attttgcccg acaacatcac caccgaccac ctctcgccgt
    19981 ccaatgcgat tttggccgtc agtgccgcag gcgagtattt ggcgaaaatg ggtttgcctg
    20041 aagaagactt caactcttac gcaacccacc gcggcgacca cttgaccgcc caacgcgcta
    20101 ccttcgccaa tccgaaactg tttaacgaaa tggtgaaaaa cgaagacggc agcgtgcgcc
    20161 aaggctcgtt cgctcgcgtc gaacccgaag gcgaaaccat gcgcatgtgg gaagccatcg
    20221 aaacctatat gaaccgcaaa cagccgctca tcatcattgc cggtgcggac tacggtcaag
    20281 gctcaagccg cgactgggct gcaaaaggcg tacgcctcgc cggcgtggaa gcgattgttg
    20341 ccgaaggctt cgagcgtatc caccgcacca acttgatcgg tatgggcgtg ttgccgttgc
    20401 agttcaaacc gggtaccaac cgccataccc tgcaactgga cggtacggaa acctacgacg
    20461 tggtcggcga acgtacaccg cgctgcgacc tgaccctcgt gattcaccgt aaaaacggcg
    20521 aaaccgttga agttcccgtt acctgccgcc tcgataccgc agaggaagtg ctggtgtacg
    20581 aagccggcgg cgtgctgcaa cggtttgcac aagacttttt agaaggtgtg gtagcataaa
    20641 ggtagacggt cttttttatg atttatgaga agaccgtttg aaaattattt atggttaaat
    20701 ttttaattta aattaaacag taaatatgaa gacacaagaa gatgaggtaa ctaagcagaa
    20761 aatttttaat catatgatta ataataaatc atttttaaat ttccagtttt ggacatttgc
    20821 tgctttgagt gcaggttcat ttgcctttct aataagtgca gcaaatatag attctaaaga
    20881 tagagacttg acgcttgaaa tagccattgt tggatattca gtaagtttaa tattaaattc
    20941 catactatgt tttttaataa gaggcataga agacgatgag ttatcggtta aaatgttaaa
    21001 tgaatttaat ttggataagc gtactttttg gattcccatt ctagcaacgt ggagttttgc
    21061 gctttcgaca cttttcttat tgtttaccat tagtctagta ggagcaatta ctgctcttat
    21121 aatggctgtt ttagcaataa ttagttttat aaaatttaca aaaaaatcat agatttttac
    21181 aatttgaagt ggtgcgtaaa tcgggcattg atgcccgaca cagaagttgt aggttgagtt
    21241 aagccccggc attttgctaa atccaacggt tttttcgggt ttgcaaccca acctacctct
    21301 gctgctaatc tgcatcatac aaaggagaac tcgaaatgcc gcaaattaaa atccccgccg
    21361 tttactaccg cggcggtaca tctaaaggca ttttcttcaa acgcaccgac ctgcccgaag
    21421 cggcgcaaca agcaggcgag gcacgcgaca aaatcctgtt gcgcgtactc ggcagcccgg
    21481 atccctacgg caagcagata gacggtttgg gcaacgccag ttcgtccacc agcaaagccg
    21541 tgattttgga caagtccgaa cgcgccgatc acgatgtcga ttaccttttc gggcaagttt
    21601 ccatcgacaa accttttgtc gattggagcg gcaactgcgg caacctcacc gctgccgtgg
    21661 gcgcattctc catcgaacag ggcttggtcg ataaaggcaa gattccttca gacggcatct
    21721 gcacggtcaa aatctggcag aaaaacatcg gcaaaaccat tattgcccat gtaccgatgc
    21781 aaaacggcgc agttttggaa acaggcgatt ttgagctcga cggcgtaacg ttcccggcag
    21841 ccgaagtaca aatcgaattt cttgatccag ccgacggcga aggcagtatg ttcccaaccg
    21901 gcaatttggt cgatgaaatt gatgtgccga atataggccg tttgaaagcc acgctcatca
    21961 acgcgggcat tccgaccgtt ttcttgaatg ccgccgactt gggctacaca ggcaaagagt
    22021 tgcaagacga catcaacaac gatgccgcgg ctttggaaaa attcgagaaa atccgcgctt
    22081 acggtgcgct gaaaatgggt ctgatcagcg acgtatccga agctgccgct cgcgcgcaca
    22141 cgccgaaagt cgccttcgtc gcgcccgccg ccgattacac cgcctccagt ggcaaaaccg
    22201 tgaacgccgc cgacatcgat ttgctggtac gcgccctgag catgggcaaa ctgcaccacg
    22261 cgatgatggg taccgcctct gttgccattg cgaccgccgc cgccgtaccc ggtacgctgg
    22321 tcaaccttgc cgcaggcggc ggaacgcgta aagaagtgcg cttcgggcat ccttccggca
    22381 cattgcgcgt cggtgcagcc gccgaatgtc aggacggaca atggacggcc accaaagcgg
    22441 tcatgagccg tagcgcacgc gtgatgatgg aaggttgggt cagggtgcct gaggattgtt
    22501 tttaaattga cgtagcatgg gtttgcccgc gagccataaa aaggtcgtct gaaaaacaag
    22561 taaacatcaa atcactgacc attcctttcc cttgccctgt ggcggaaggc ggcaaatcac
    22621 aaggaagaac acggaaaccc cgataaaaga cagcttcccg tattaccgtc attcccgcgc
    22681 aggcgggaat ccagacctgt caatatggag gattggcagg ggaaaacagg tttcgtgagt
    22741 tctacattct ggattcccgc cacagcctgt cctcgcgtag gcggggacgg aataacgata
    22801 gaaaatgcgg catatgcttt gcccaaagag gccgtctgaa acacactccg cccaacgccc
    22861 atcctttttc agacgacccc acaccaaaaa acaaccacaa actacaagga gaaacatcat
    22921 gtccgaccaa ctcattcttg ttctgaactg cggcagttca tcgctcaaag gtgccgttat
    22981 cgaccgcaaa agcggcagcg tcgtcctaag ctgcctcggc gaacgcctga ccacgcccga
    23041 agccgtcatt acgttcagca aagacggcaa caaacgccaa gttcccctga gcggccgcaa
    23101 ctgccatgcc ggcgcggtgg gtatgctgct gaacgaactg gaaaaacacg gactgcacga
    23161 ccgcatcaaa gccgtcggcc accgcatcgc ccacggcggc gaaaaataca gcgagtctgt
    23221 tttgatcgac caggccgtaa tggacgaact caatgcctgc attccgcttg cgccgctgca
    23281 caaccccgcc aacatcagcg gcatccttgc cgcacaggaa catttccccg gtctgcccaa
    23341 tgtcggcgtg atggatactt cgttccacca aaccatgccg gagcgtgcct acacttatgc
    23401 cgtgccgcgc gagttgcgta aaaaatacgc tttccgccgc tacggtttcc acggcaccag
    23461 tatgcgttac gttgcccctg aagccgcacg catcttgggc aaacctctgg aagacatccg
    23521 catgattatt gcccacttag gcaacggcgc atccattacc gccatcaaaa acggcaaatc
    23581 cgtcgatacc agtatgggtt tcacgccgat cgaaggtttg gtaatgggta cacgttgcgg
    23641 cgacatcgat ccgggcgtat acagctatct gacttcccac gccgggatgg atgttgccca
    23701 agtggatgaa atgctgaaca aaaaatcagg tttgctcggt atttccgaac tttccaacga
    23761 ctgccgcacc ctcgaaatcg ccgccgacga aggccacgaa ggcgcgcgcc tcgccctcga
    23821 agtcatgacc taccgcctcg ccaaatacat cgcttcgatg gctgtgggct gcggcggcgt
    23881 tgacgcactc gtgttcaccg gcggtatcgg cgaaaactcg cgtaatatcc gtgccaaaac
    23941 cgtttcctat cttgatttct tgggtctgca catcgacacc aaagccaata tggaaaaacg
    24001 ctacggcaat tcgggcatta tcagcccgac cgattcttct ccggctgttt tggttgtccc
    24061 gaccaatgaa gaactgatga ttgcctgcga cactgccgaa cttgccggca tcttgtagcc
    24121 aaaaaaggga cgagtccgca aaaatgccgt ctgaaatttt cagacggcat tttgtttgga
    24181 ttttgggcgt acaatacgga aaacatgacg ataaggacac aaactatggc acagtttttc
    24241 gccgttcatc ccgacaaccc ccaagaacgc ctcatcaagc aggcggtcga aatcgtcaat
    24301 aaaggcggcg tggtcgttta tccgaccgat tcctgttatg ccttgggctg caaactcggc
    24361 gataaggcgg cgatggaacg catactctcc atccgcaaaa tcgatttgaa acaccacctg
    24421 accctgatgt gcgcagattt gagcgagttg ggcacatacg ccaaagtcga caacgtacag
    24481 tttcgtcagc ttaaagccgc cacacccggg ccttatactt ttattttaca ggcgacgaag
    24541 gatgtgccgg cgcgcacgct gcacccgaaa cgcaaaacca tcgggctgcg tattcccgat
    24601 aatgccattg cacaagccct gctgggggaa ttgggcgagc cgcttttaag ctgcaccctg
    24661 atgctgcccg aagacggcga accattgacc gatccttatg aaatccgcga gcgtttggaa
    24721 cacgccgtcg atttggtgat tgacggcggc tggtgcggaa ccgagccgac caccgtcgtc
    24781 gatatgaccg acggcacgga attggtgcgc caaggttgcg gcgatacggc ggtgttcggt
    24841 ttgtagggaa accgatgccg tctgaagcat cggctgttca gacggcattg cgcgccttgc
    24901 cggcggcagt ccgaaatgcc ggcgcgtatc gcgctcggtc ggaatatccg tttgaaacgg
    24961 cattttgatg cattactgca ccgcaatcgg aattctcggt tcgtagagca ggtcgtaggt
    25021 cggcttgttg agcaggtctt ggagcgtgaa accgtccaga tacgtgaaaa acgacttcat
    25081 cgcgccgccg agtatgcccg tcagccggca ggacggtgta atcaggcatt cgttgttctc
    25141 gcccatgcac tcgaccagct gcatcggttc gaggtggcgg acaaccgagc cgatgttgat
    25201 gcggtcgggc ggtgcggcaa gccgcagacc gccgcctttt ccgcgcacac tgtggaggaa
    25261 gccgcctttg accagcgcgg taacgacctt catcagatgg cttttggaaa tgccgtaggt
    25321 tacggcgatg gtactgatgt tgaccagcgc atcgtcgttg atggcagtgt agataaggac
    25381 gcgcagcccg tagtccgtat gttgtgtcaa atacatgatt ttctcggtat ggattgttat
    25441 tcttatcggt acggtttaag gttcacggac aataccttaa tggttgaaac cctgtccgtc
    25501 ggggcggtag aatgcagcct gtctgcggcg gtatgccgtc tgaaacatcc gcgctaccgt
    25561 ttgagaattt gttattgtaa ctcaaaatca tgaaaccgtt gaaacgacat cccgccctta
    25621 tcgggctttc gcgtgaccac caccattcgc tttccctgtg cgtgcgtctg ttgcggacgc
    25681 cggaagaaag gcatcgggac gaactcgaac cgcatttttc cgaattggaa acccattttc
    25741 gcgaagaaga agccaagttt gccccaattt ggcagaatgt cgcccccgaa ttgaaacaac
    25801 gtttcgagaa agaccacgcc cgactgcggc agatgatggc aagccccgaa tacggtaacg
    25861 cggcgtggaa taccgctttt gccacaaccc tgcgcgacca cgcgcgcttt gaagaacgcg
    25921 agctgtttcc cgccgccgaa ccgtttttgc cggcatgatt ccgttttgcg gcaaatatat
    25981 taatgataaa caaggaacac acatgaaatt taccaagcac cccgtctggg caatggcgtt
    26041 ccgcccattt tattcgctgg cggctctgta cggcgcattg tccgtattgc tgtggggttt
    26101 cggctacacg ggaacgcatg agctgtccgg tttctattgg cacgcgcatg agatgatttg
    26161 gggttatgcc ggactggtcg tcgtcgcctt cctgctgacc gccgtcgcca cttggacggg
    26221 gcagccgccc acgcggggcg gcgttctggt cggcttgact atcttttggc tggctgcgcg
    26281 gattgccgcc tttatcccgg gttggggtgc gtcggcaagc ggcatactcg gtacgctgtt
    26341 tttctggtac ggcgcggtgt gcatggcttt gcccgttatc cgttcacaga atcaacgcaa
    26401 ctatgttgcc gtgttcgcgc tgttcgtctt gggcggcacg cacgcggcgt tccacgtcca
    26461 gctgcacaac ggcaacctag gcggactctt gagcggattg cagtcgggct tggtgatggt
    26521 gtcgggtttt atcggtctga ttggtacgcg gattatttcg ttttttacgt ccaaacggtt
    26581 gaatgtgccg cagattccca gtccgaaatg ggtggcgcag gcttcgctgt ggctgcccat
    26641 gctgaccgcc atgctgatgg cgcacggcgt gatgccttgg ctgtcggcgg ctttcgcgtt
    26701 tgcggcaggt gtgattttta ccgtgcaggt gtaccgctgg tggtataaac ccgtgttgaa
    26761 agagccgatg ctgtggattc tgtttgccgg ctatctgttt accggattgg ggctgattgc
    26821 ggtcggcgcg tcttatttca aacccgcttt cctcaatctg ggtgtgcatc tgatcggggt
    26881 cggcggtatc ggcgtgctga ctttgggcat gatggcgcgt accgcgctcg gtcatacggg
    26941 caatccgatt tatccgccgc ccaaagccgt tcccgttgcg ttttggctga tgatggcggc
    27001 aaccgccgtc cgtatggttg ccgtattttc ttccggcact gcctacacgc acagcatacg
    27061 cacctcttcg gttttgtttg cactcgcgct tttggtgtat gcgtggaagt atattccttg
    27121 gctgattcgt ccgcgttcgg acggcaggcc cggttgagac aaaccgccgc agatttcggg
    27181 tctgggcggt ttgcttttca gacggcaggg cggtcagttg ccgtccagcc agcggtcgcg
    27241 tgtggttttg gcttcttcaa aatagcggta cagggcttcg cggtcgtcgg tggtcaggat
    27301 gtttgccaaa acgtccaact gtttgcccaa gccttgaacc agttgcagca ggctgtcttt
    27361 gttggcaagg cagatgtccg cccacacggc gggatgaccg gaggcgatgc gggtgaagtc
    27421 ccgaaagccc gtggcggcga atttcagata ttcctgtccg tcggggtggt cgagaatctg
    27481 gtggacatag gcgaaggcgg tcaggtgggg catatgggag acggcagcga aaaccgcgtc
    27541 gtggcgttgc gcgtccatcg tataaatttc cgcaccgacc gcgtgccaca gttcttctac
    27601 caaggcaatg ccgtctgaat gttcgccgcc gtgtggcgtg atgatgagtt ttctgtggcg
    27661 gaacagcccg aactgcgcgg cttgcgcacc gcttctgtcc gaaccggcaa tcgggtgggc
    27721 ggcgatgcag cggtgcaggc ggtcgggcag acagcggcgg aaggcttcga tgaccgaaga
    27781 tttggtgctg ccgacatcgg aaatccaagt gtgttccggc aaaacggggc gcagcgcggt
    27841 caaaatggcg ggaaaggtgg cgacgggcgt ggcaatcagt accaagtccg caccgccgat
    27901 gctgtccgcg tcgatggcaa cggaggcacg gtcaatcacg ccgcgttcca atgcacgttc
    27961 gaggttgtcg cggtcggtgt cgataccggt aacggtgcgg acgagtccct gccttttgag
    28021 gtcgagaacg aacgaaccgc cgatcagccc tacaccgatg agggcaatat ggttcaaaat
    28081 gggcatttgt gtaaacggtt ttcgcaaagt accgtcatgg tagcctatcg gcggaatatg
    28141 ccgcaaggtc ggcaggaaaa aggagaagaa atggacaaaa tcagagttgc cgccgtgcag
    28201 atggtgtcgg gcgtgtcgcc ggaaaccaac gtcgctgcca tgaaacgcct ggtcgcacgg
    28261 gcggcggagc agggtgtgga ttgggtgctg ctgcccgaat attgggtgct gatgggcgca
    28321 aacgataccg acaaactcgc gcttgccgag cctttgggcg gcggacgctt tcagacggca
    28381 ttgagcgaaa cggcgaaaga atgcggcgtg gtgctgtttg gcggaaccgt gccgctgcaa
    28441 agccccgagg cgggcaaggt catgaatact ttattggtgt acgggcgcga cggcaaacgg
    28501 acggggctgt accacaaaat gcacctcttc ggcttttccg gtttgggcga acgctatgcc
    28561 gaagccgata ccatccgcgc gggcggggaa gtgccgcact tgtcggcaga gggcgtgccg
    28621 gtggcagcag gtatttgtta cgatgtccgc tttcccgaat ttttccgccg ccagctgccg
    28681 tttgacgtat tgatgctgcc cgctgcgttt acgcacacga cgggcaaggc gcattgggag
    28741 ctgctgctgc gcgcgcgtgc cgtcgaaaac caatgttacg tcgtggcggc ggcacagggc
    28801 ggtttgcacg aaaacggacg gcgcacgttc ggacacagca tgattgtcga tccgtggggc
    28861 gacgtgttgg acgtattgcc cgaaggcgaa ggcgttgtta cggcagacat cgatgccaac
    28921 cgcctgaaca gcgtccgcaa ccgcctgccc gccttgaaac actgggtttt ggatgccgtc
    28981 tgaaggttca gacggcatcg gtgccgggga atcagcgggc tgtaggatgg gcttcagcct
    29041 gccatttcca tcaatcccac atttctaccg ttttcatcga atccatcgaa tccgcccctt
    29101 tcgacgcccc agtcctacgc aactgaaccg tcattcccac gaaagtggga atctagaacg
    29161 cggggtttgg gcaactgttt ttatccgata agtttctgtg cggacaggtt tggattcccg
    29221 cctgtgcggg aatgacaggt ttcgagattg cggtgttgtc ggaacgcaac tgaatcgtca
    29281 ttcccacgga agtgggaatc tagaatctcg gggtttcagt catttccgat agattcccgc
    29341 cgcgtcgtgg gtctggattc ccgcctgtgc gggaatgacg aatttc
//
LOCUS       DAXHMX010000007        27746 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHMX010000007 DAXHMX010000000
VERSION     DAXHMX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.007"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            169..792
                     /gene="lolA"
                     /locus_tag="ACFM4S_000224"
     CDS             169..792
                     /gene="lolA"
                     /locus_tag="ACFM4S_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6049987.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1065..2204
                     /locus_tag="ACFM4S_000225"
     CDS             1065..2204
                     /locus_tag="ACFM4S_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6049988.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAGQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2336..3385
                     /locus_tag="ACFM4S_000226"
     CDS             2336..3385
                     /locus_tag="ACFM4S_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6049989.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKTTNNKQQTTNNKQQTTNNKQQTTNNTRYRTPNIKYIYQDNQGTAEAR
                     NTGIKNSNGKYIVFIDSDDFINCQVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGE
                     DYQPEKILKQSKVEVLKGLCRFRKFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWS
                     MRLFNAATTFSYLDGCYYYYRQGRKDSKTGTVSEKSIKSLLYILEKNAEMEFDRDISS
                     YLYSFLSYEYLVLLFIMTSKNIECDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFG
                     VDITGRILKAIRGNI"
     gene            3426..3956
                     /locus_tag="ACFM4S_000227"
     CDS             3426..3956
                     /locus_tag="ACFM4S_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6049990.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(3990..4106)
                     /locus_tag="ACFM4S_000228"
     CDS             complement(3990..4106)
                     /locus_tag="ACFM4S_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6049991.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(4321..5916)
                     /locus_tag="ACFM4S_000229"
     CDS             complement(4321..5916)
                     /locus_tag="ACFM4S_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6049992.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVKPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(6023..6418)
                     /gene="hisI"
                     /locus_tag="ACFM4S_000230"
     CDS             complement(6023..6418)
                     /gene="hisI"
                     /locus_tag="ACFM4S_000230"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6049993.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(6449..7216)
                     /gene="hisF"
                     /locus_tag="ACFM4S_000231"
     CDS             complement(6449..7216)
                     /gene="hisF"
                     /locus_tag="ACFM4S_000231"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6049994.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(7229..7966)
                     /gene="hisA"
                     /locus_tag="ACFM4S_000232"
     CDS             complement(7229..7966)
                     /gene="hisA"
                     /locus_tag="ACFM4S_000232"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6049995.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQTVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(7999..8637)
                     /gene="hisH"
                     /locus_tag="ACFM4S_000233"
     CDS             complement(7999..8637)
                     /gene="hisH"
                     /locus_tag="ACFM4S_000233"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6049996.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVWTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCIAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDRQGCRLKVPHMGWNTVRQTQNHPLFKDIPQNTRFYFVHSYY
                     FDPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(8768..10270)
                     /gene="pta"
                     /locus_tag="ACFM4S_000234"
     CDS             complement(8768..10270)
                     /gene="pta"
                     /locus_tag="ACFM4S_000234"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6049997.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(10455..11513)
                     /gene="fbpC"
                     /locus_tag="ACFM4S_000235"
     CDS             complement(10455..11513)
                     /gene="fbpC"
                     /locus_tag="ACFM4S_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6049998.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(11534..13051)
                     /gene="fbpB"
                     /locus_tag="ACFM4S_000236"
     CDS             complement(11534..13051)
                     /gene="fbpB"
                     /locus_tag="ACFM4S_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6049999.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(13119..14114)
                     /gene="fbpA"
                     /locus_tag="ACFM4S_000237"
     CDS             complement(13119..14114)
                     /gene="fbpA"
                     /locus_tag="ACFM4S_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6050000.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(14365..14835)
                     /locus_tag="ACFM4S_000238"
     CDS             complement(14365..14835)
                     /locus_tag="ACFM4S_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFC6050001.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(14892..16268)
                     /gene="argH"
                     /locus_tag="ACFM4S_000239"
     CDS             complement(14892..16268)
                     /gene="argH"
                     /locus_tag="ACFM4S_000239"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6050002.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(16287..17156)
                     /gene="galU"
                     /locus_tag="ACFM4S_000240"
     CDS             complement(16287..17156)
                     /gene="galU"
                     /locus_tag="ACFM4S_000240"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6050003.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(17184..17783)
                     /gene="rdgB"
                     /locus_tag="ACFM4S_000241"
     CDS             complement(17184..17783)
                     /gene="rdgB"
                     /locus_tag="ACFM4S_000241"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6050004.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELAGKTDKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(17776..18009)
                     /locus_tag="ACFM4S_000242"
     CDS             complement(17776..18009)
                     /locus_tag="ACFM4S_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6050005.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(18117..18650)
                     /locus_tag="ACFM4S_000243"
     CDS             complement(18117..18650)
                     /locus_tag="ACFM4S_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6050006.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(18752..19210)
                     /gene="nudB"
                     /locus_tag="ACFM4S_000244"
     CDS             complement(18752..19210)
                     /gene="nudB"
                     /locus_tag="ACFM4S_000244"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6050007.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            19421..19497
                     /locus_tag="ACFM4S_000245"
     tRNA            19421..19497
                     /locus_tag="ACFM4S_000245"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19455..19457,aa:Pro,seq:ggg)
     gene            19655..21481
                     /gene="mafB"
                     /locus_tag="ACFM4S_000246"
     CDS             19655..21481
                     /gene="mafB"
                     /locus_tag="ACFM4S_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6050008.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLFIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSNNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            21484..21948
                     /locus_tag="ACFM4S_000247"
     CDS             21484..21948
                     /locus_tag="ACFM4S_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050009.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            22173..22319
                     /locus_tag="ACFM4S_000248"
     CDS             22173..22319
                     /locus_tag="ACFM4S_000248"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050010.1"
                     /translation="MWYEANIGLINTMSRSNQAGTRLLYSNYGLLYITTDHYISATRF
                     VAWK"
     gene            22331..22618
                     /locus_tag="ACFM4S_000249"
     CDS             22331..22618
                     /locus_tag="ACFM4S_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFC6050011.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            23132..24370
                     /locus_tag="ACFM4S_000250"
     CDS             23132..24370
                     /locus_tag="ACFM4S_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6050012.1"
                     /translation="MIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEALKKDKQ
                     TARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKESLYYLN
                     KHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGSCAETFG
                     LGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEYVQFSID
                     LFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAH
                     AQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVE
                     IKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSK
                     SGIKIEGFTEPNRTAYPIYE"
     gene            24401..24820
                     /locus_tag="ACFM4S_000251"
     CDS             24401..24820
                     /locus_tag="ACFM4S_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050013.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            24857..25831
                     /gene="mafB"
                     /locus_tag="ACFM4S_000252"
                     /pseudo
     CDS             24857..25831
                     /gene="mafB"
                     /locus_tag="ACFM4S_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            25842..26303
                     /locus_tag="ACFM4S_000253"
     CDS             25842..26303
                     /locus_tag="ACFM4S_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050014.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            26290..26577
                     /locus_tag="ACFM4S_000254"
                     /pseudo
     CDS             26290..26577
                     /locus_tag="ACFM4S_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
     gene            26604..27023
                     /gene="mafI"
                     /locus_tag="ACFM4S_000255"
     CDS             26604..27023
                     /gene="mafI"
                     /locus_tag="ACFM4S_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6050015.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            27113..27184
                     /locus_tag="ACFM4S_000256"
                     /pseudo
     CDS             27113..27184
                     /locus_tag="ACFM4S_000256"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            27181..27423
                     /gene="imm31"
                     /locus_tag="ACFM4S_000257"
     CDS             27181..27423
                     /gene="imm31"
                     /locus_tag="ACFM4S_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC6050016.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            27501..27746
                     /locus_tag="ACFM4S_000258"
     CDS             27501..27746
                     /locus_tag="ACFM4S_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6050017.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
BASE COUNT         6958 a         6330 c         7658 g         6800 t
ORIGIN      
        1 cggcacacat taaagggcag cgtggcgcgc ctgccttttc cgagcggtca aaaaaatcag
       61 ccctcggaaa acgctgtttg taaaatgcaa accgcccgta acgccgcccg tatgattgtt
      121 ttgccgcgcc gatactttac gccacaccca tcccaacaag gaaaaataat gatgaaaccg
      181 cacaacctgt tccaatttct cgccgtttgc tccctgaccg tctccgtcgc ttccgcacag
      241 gcgggcgcgg tggacgcgct caagcaattc aacaacgatg ccgacggtat cagcggcagc
      301 ttcacccaaa ccgtccaaag caaaaagaaa acccaaaccg cgcacggcac gttcaaaatc
      361 ctgcgcccgg gcctcttcaa atgggaatac acttcgccct acaaacagac tattgtcggc
      421 gacggtcaaa ccgtttggct ctacgatgtc gatttggcac aagtgaccaa gtcgtcccaa
      481 gaccaggcca taggcggcag ccccgccgcc atcctgtcga acaaaaccgc cctcgaaagc
      541 agctacacgc tgaaagagga cggttcgtcc aacggcatcg attatgtgct ggcaacgccc
      601 aaacgcaaca atgccggcta ccaatacatc cgcatcggct tcaaaggcgg caacctcgcc
      661 gccatgcagc ttaaagacag cttcggcaac caaacctcca tcagtttcgg cggtttgaat
      721 accaatcccc aactctcgcg cggcgcgttc aagtttaccc cgcccaaagg cgtggacgtg
      781 ttgagcaact gatgccgtcc gcctcgatgc cgtctgaatg ccgccgaggc ttcagacggc
      841 attttcacgc aggcggaaca atgtcccgca ttaccgcccg atcgggcatc ggaacggcaa
      901 acccgtgaaa attaacggtt gcgccccggt tgtttttgcc gtttaatgca aaccttgctg
      961 caccaagggc caagaaagcc gaccggccgc ccccacagct tccgatgcag gcggcccgtc
     1021 cgtccctgca atgtttttta tttttgaacg aaaggtcgaa aaccatgaaa aaaacactgg
     1081 tggcggcggc gatcctgagc ctcgccttga ctgcgtgcgg cggcggaagc gataccgccg
     1141 cccaaacccc ctccgccaag cccgaagccg gacaatcggg caaactcaat atctacaact
     1201 ggtcggatta tgtcgatccc gaaaccgtcg ccgcctttga aaaagaaacc ggcatcaaga
     1261 cgcgttccga ttattacgac agcaacgaaa cactggaggc aaaagtcctg accggcaaat
     1321 ccggctacga cctgaccgcg ccgtccatcg ccaacgtcgg ccggcaaatc aaagcgggtg
     1381 cgtatcagaa aatcgacaag gcgcaaatcc cccattacgg caacatcgat aaagatttgc
     1441 tgaaaatgat ggaagccgtc gatccgggca acgaatatgc cgtgccttat ttctggggaa
     1501 tcaatacttt ggcaatcaat acccagcagg tgaaaaaggc attgggtacg gacaagctac
     1561 ccgaaaacga gtgggatttg gtgttcaaac ccgaatacac cgccaaactc aaatcctgcg
     1621 gcatcagcta tttcgacagc gcaatcgaac agattccctt ggcgttgcac tatttgggca
     1681 aagaccccaa cagtgaaaat cctgaagaca tcaaagccgc cgtcgatatg atgaaagccg
     1741 tccggggcga cgtgaaacgc ttcagctctt ccggctatat cgacgatatg gcggcgggca
     1801 acctgtgtgc cgccattggt tacggcggcg atttgaacat tgccaaaacc cgtgccgaag
     1861 aagccgcaaa cggcgtggaa atcaaagtat tgaccccgaa aaccggcgtg ggcgtgtggg
     1921 tggattcctt tatgattccg cgcgacgcgc aaaacgtcgc caacgcccac cgctatatcg
     1981 actacacgct ccggcccgag gtggcggcga aaaacggcag cttcgttacc tacgcgcccg
     2041 ccagccgtcc cgcgcgcgag ctgatggatg aaaaatacac ctccgacgca tcgattttcc
     2101 cgaacaaaga actgatggaa aaaagtttca tcgtatcgcc caaatccgca gaatccgcca
     2161 aactgggcgt gaagctgtgg caagggctca aggccggaaa ataaccgcaa cctctgccgt
     2221 ctgaaacctt tcgggcggca ggaaacggcg cgtccgttat caaacagggg gcgtttcccc
     2281 gcccgcctgt tatgattggg ttaagattaa aatgatttag taaaatgaga aagatatgga
     2341 tttaagtatc gtagttccca tttataatgt cgagagttat ttggaagcgt gtctaaattc
     2401 catagaacct atattaagta atgaaaatgt cgaacttatc cttgtgaatg acgggtcaaa
     2461 agacggaagt gaagatatat gttataaata tatagataaa acaacaaaca acaaacaaca
     2521 aacaacaaac aacaaacaac aaacaacaaa caacaaacaa caaacaacaa acaacaccag
     2581 ataccgaaca ccaaacatca aatatatata tcaggataac caagggacgg cagaagctag
     2641 gaataccgga ataaaaaatt caaacggaaa atatatagtc tttattgatt cggatgattt
     2701 tattaattgt caggtcttgc tggattttct tagtaaagat gatattgata tgccggatgt
     2761 ggttttttta aatgcggtta aatatgataa gggaagtgtt tcacattttg gcgaagatta
     2821 tcagcctgaa aaaatactca agcaatccaa agtcgaagtt ttgaaaggat tatgccgatt
     2881 tagaaaattt ccgggttcgg cgtgtaataa gattataaaa agggaattga ttatcaaaga
     2941 aaaactgttt tttgaaaaag aggtttatgc cgaagatatc gaatggtcaa tgagattatt
     3001 taatgcggca acaacttttt cttatttgga cggttgttat tactattatc ggcagggaag
     3061 aaaagattct aaaacaggaa ctgtttcgga aaaaagtata aagtcattat tatatatttt
     3121 ggagaaaaat gcggaaatgg aatttgatag ggatatatca agttatcttt attcttttct
     3181 ttcctacgaa tatctcgttt tgctttttat catgacgagt aaaaatatag agtgtgatgc
     3241 tgatataaaa agaagggcgt atcatttaag gtttatgctg ttaaagtcca ataaattgat
     3301 atataagctg atattcccga taatcacatt attcggggtc gatattacag gcaggatttt
     3361 aaaagcaatc agggggaata tttaataaat cctttaacac tatacatctt accgaaggag
     3421 gaaaaatgaa cgcaatccga actttccaaa accgcacgcc cgaaatccac gaaagctgta
     3481 tgatagacga agcgtgcgtc gtcatcggcg aagtgtcgct tgccgaagac gtttccgtgt
     3541 ggccgtgcgc cgtgttgcgc ggcgatgtga acagcatcac cgtcggcgcg cgcagcaata
     3601 tacaggacgg cagcgtcctg cacgtttccc acaaaaccgc cgccaaaccc gaagggtcgc
     3661 cgctggttat cggcgaagac gttaccgtgg ggcacaaagt gatgctgcac ggctgccgta
     3721 tcggcaaccg cgtcctggtc ggcatgggga cgacggttct ggacgatgcc gtgattgagg
     3781 acgaagtgat gatcggcgcg ggcagcctcg tgcctccgcg caaacgtttg gagggcggct
     3841 atctttatgt cggttcgccg gtcagacaag tgcgcgtgtt gaccgatgag gaaaaagcct
     3901 ttttgaagta ttccgccgcg cattatgtga agctgtcgaa acagtacggg atgtgaaatc
     3961 acatcggcgt tcttgcgtca gccccaaatt catgcggatg ggacgcatcc gataacggta
     4021 tccgatgcgc cttgattttg accgtctgcg tttgagttgc aggcaaaaat gccgtctgaa
     4081 agcctttttt cgggttcaga cggcatttta tcgcctaaag tttgaccgaa tgttcgcgcg
     4141 tttcgtggaa cacgatgtcc ggccagcgtt cttgcgtcag ccctaaattc atgaggacgg
     4201 gatgcccgat aacggtatcc gatgcgtctt gattttgatc ggtgcatttg agttgcaggc
     4261 aaaaatgccg tctgaaagcc ttttttcggg ttcagacggc attttatcgc cgattgcttt
     4321 ttacagttta accgaatgtt cgcgcgtttc gtggaacacg atgtccggcc aacgttcttg
     4381 cgtcaacccc aaattcacgc ggttgggggc gaggtaggcg aggttgccgc ccgcgtcgat
     4441 ggcgaggttg cccgcgttgg ctttttcaaa ctccgccagt tttttcttgt cgtcgcacga
     4501 tacccagcgc gccgaccaga tggatgcgct gtcgaacacg gcttctacgc cgtattcgtt
     4561 ggccagccgc gaggtaacga cttcaaactg caacacgccg accgcgccca aaatcaaatc
     4621 cgcgccgctc atcggtttga atacctgtac cgcgccttct tcgccaagct gttgcaaacc
     4681 tttttgcagt tgcttgattt tcagcgggtt tttgatgcgg acgctgcgga acagttcggg
     4741 cgcaaagaat gggatgccgg taaacgccag ttgttcgcct tcggagaagc tgtcgccgat
     4801 ttggatgttg ccgtggttcg ggatgccgat gatgtcgccg gcgtaggctt cttccaccag
     4861 ctcgcggtcg tgcgacataa aggttaccac gctggaggca gcgatttcgc ggttgatgcg
     4921 caggtgtttc atcttcatac cgcgctcgaa tttgccggag cagacgcgca agaaggcgat
     4981 gcggtcgcgg tgtttcggat ccatattggc ttggatttta aagataaatc cggagaattt
     5041 cggctcgtcc ggcttgacca tacgtacggt cgcgtcgcgc ggtttcggcg cgggcgccca
     5101 gtcaatcaat gaattgagga tttcctgaat accgaagttg ttaatcgcag agccgaagaa
     5161 tacgggcgtg agttcgccgg cgaggaattc gtcgagatta aactcgttgg aagccgcctg
     5221 caccaattcg atttcgtcgc gcaactgctg gatttccaat ggaaagcgtt gttccaattc
     5281 gggattgtcg atgcctttga tgatgtcgaa ctcgtgcggc agacgttcgc caccggcttc
     5341 aaagagatag atttcgtcgt tcaggatgtg gtacacgcct ttgaagtttt tgcccatgcc
     5401 gatcggccag gttacgggcg cgcagcggat ttgcaggatg ttttccactt cgtccagcaa
     5461 ctccaaagaa tcgcgcactt cgcggtcgta tttgttcatg aacgtgacaa tcggcgtatt
     5521 gcgcaggcgg cagacgttca agagtttgat ggtttgcgct tccacacctt ttgccgcgtc
     5581 gatgaccatc aaggcgctgt ccacggcggt cagaacgcgg taggtgtctt cggagaagtc
     5641 ttggtgtccc ggcgtgtcca agaggttgac ggtgtggtct ttgtagtcga actgcatcac
     5701 gcttgatgcc acggaaatgc cgcgctgctt ctcgatgtcc atccagtcgg aggtggcgaa
     5761 tttgccggtt tttttgcctt ttaccgtacc cgcgctctga atcgcgcccg aaaacagcaa
     5821 cagcttttcg gtcagcgtgg ttttacccgc gtcggggtgg gagatgatgg caaacgtgcg
     5881 gcggcggcgc acttggtcga ggatttcttg ggacatggtt ttctttgcaa aaaggttcag
     5941 gccgcttttc agacggcccg gacagtgttt gagacggcga aattgtacaa aaaaatgcct
     6001 gataattcaa tgttggaggc ggtcagtgcg tgctgccgta aatctctttt tcgtctttca
     6061 ggacggcatc ggcggtttcc cacgcgccgc cgcgccagac tttgtaaaag cagctttctc
     6121 gcccggtgtg gcaggcgatg ccgccgtttt gggcgatgag catcacaatg gcgtcgccgt
     6181 cgcagtcgag gcgcagttcg cggacttttt gcgtgtgtcc cgactcttcg cccttcatcc
     6241 attgtttttg gcgcgaacgg ctgtaatagt gggcaaagcc ggtttcgacg gttttttgca
     6301 gggcttcggc gttcatccac gctaccatta aaacacgttt ggtttcggca tcttgggcga
     6361 tggcgcaaac caaacctttt tcgtcaaatt tgacggcttc aagcaggttt ttatccatat
     6421 ttcctttcag acggcatagt cggggcggtc agaggcgcac ttcgatgccg gcttcgcgca
     6481 tagcgcgttt ggcttcgcgg atggcgattt ccccgaaatg gaaaacgccg gcggcaagta
     6541 cggcatcggc tttgccttcg gttatgcctt caatcaggtg ccggacattg ccgaccccgc
     6601 cggaggcgat gacggggatg tcgacggctt cggcaacggc gcgggtcagc ggcaggttga
     6661 aaccctgttt cgtaccgtcc ctgtccatac cggtaagcag gatttcgccc gcgccgcgtt
     6721 tttgcatttc gatcgcccat tccaccgcat ccaaaccggt cggattgcgc ccgccgtggg
     6781 taaagatttc ccagcgtgtg ttttcggggt tgacggcttt ggcatcgacg gcggcgacga
     6841 tggcttgcga accgaaaaat ccggcggctt cgtcaatcag gtcgggacgg gtaacggcgg
     6901 cggtgttgat gctgactttg tccgcgccgg cattgagcag gcggcggatg tcggcaacgg
     6961 tgcgtacgcc gccgccgacg gtcagcggga tgaagacttg tccggcaact tcttcgatga
     7021 tgtgcaggat ggtatcccgg ttgtcggatg aggcggtgat gtcgaggaag gtcaattcgt
     7081 ccgcgccttc gccgttgtag cgtttggcgg cttcgacggg gtcgcccgcg tcgcgcaaac
     7141 cgatgaagtt cacgcctttg acgacgcgcc cgtcttttac gtcgagacag gggatgatgc
     7201 gttttgccag tgccataatc ggatgccttt agtcgaggga atctgccagt tgctgcgctt
     7261 gggcaaaatc gatgctgccc tcgtaaatcg cgcggccggt aatcgcgcct gctacgccat
     7321 gtttttcggc ggcacacagg gcgcggatgt cgtccaagcc ggtcagtccg ccggaggcga
     7381 tgacgggaat gcggacggtt tgggcgagtt tgaccgtcgc gtcgatgttc acgccgctca
     7441 tcataccgtc gcgcccgatg tcggtgtaga tgatgctgtt gacgccgtcg tcttcaaagc
     7501 gttttgccaa atcaattaca tgatgcccgg ttacggttgc ccagccgtcg atggcggcca
     7561 taccgtcttt ggcatccagc ccgacaataa tcctgccggg gaaggctttg cacgcctcgc
     7621 gcacgaagtc ggggtttttg accgccgccg tgccgataat cacgtcgttc agacccaaat
     7681 ccaaatattg tccgatggtt ttcaaatcgc gtatgccgcc gccgagctgt acggggatgt
     7741 ctttggcgac ggcggcaagg atgtctttga tggcgggcag gttttgcgga acgccggcaa
     7801 acgcgccgtt caaatctacc agatgcaggc ggcgcgcgcc ttgtttgaac cagtgcagtg
     7861 cggtttcggc gggcgaatcg gaaaagacgg tcgcctcttc catcagccct tgtctcaggc
     7921 ggacgcagcg tccttctttc aaatcgatgg cgggtatcag cagcataatt tttctccttg
     7981 tgcggggccg tgtccggctt accagtttaa aaagtttttc aacatcgtca gcccggcatc
     8041 gtggcttttt tcggtgtgaa attgcgtggc gaatacgttg tctttgccga cgatgcaggc
     8101 aaacggggac gggtagtcgc tttcgcccaa tatggtttcg ggattttcgg ggtcgaaata
     8161 gtagctgtgg acgaagtaaa aacgcgtgtt ttggggaata tctttaaaca gcgggtggtt
     8221 ttgggtttgg cgcacggtgt tccagcccat atgcgggact ttcagacggc atccctgccg
     8281 gtcgcggagg tcgcgctcaa agcgtctgac tttgccgccg aaccagccca agccgtcggt
     8341 gtttccttct tcactgtggt cgaataaaag ttgcgcgccg acgcagattc cgaaaaaggg
     8401 tttgtttttt aaggcatctt tgactgcctc gtccaagccg ccgcgcgtca gtgccgcaat
     8461 acagtcgggc atcgcgccct gcccgggaaa aatgactttg tcggcgcggg acacgcgttc
     8521 ggggtcgccg cttaaaaaga tttcggtatt ttttccggca agctgccccg ccgtccagac
     8581 ggatttcaat acggaatgca ggttgcccat accgtaatcg ataatggcga tttgcatggc
     8641 ttcctcctct ttttttgcaa tatggctgcg attttaacaa acaaatgtgc cgtgctgata
     8701 aaaatgccgt ctgaaaacgg gagtctgtct tcagacggca tagggtttaa acccggaaag
     8761 ccgtttgtca gccttccatt tgttttgcct gaacggcagt tagggcgatg gtgaacacga
     8821 tgtcttccac cagcgcgccg cgcgacaggt cgttgaccgg tttgcgcagg ccttgcagca
     8881 gcgggccgac gcttaagacg ttggcactgc gttggacggc tttataggtg cagttgccgg
     8941 tgttcaggtc ggggaagacc aaaacggttg cctgtcctgc caccgggctg cccggagctt
     9001 tggatttgcc cacgcccggc acggttgccg catcatattg cagcgggccg tcgatggcga
     9061 ggtcggggcg tttttcccgg gcaagtttgg ttgcttcgat gacggtatcg acatcggggc
     9121 cgctgccgga gttgacggtg gagtaggaaa tcatcgccac tttcgggtcg atgccgaagg
     9181 cttttgcgga atcggcagac tggatggcga tgtcggcaag ctgttgcgcg gtcgggttcg
     9241 gattaaccgc gcagtcgccg aagacgagga cttggttggg cagcagcata aagaatacgc
     9301 tggacacgag gcttgcgccc ggtgcggttt taatcagttg caaagcgggg cggatggtgt
     9361 tggcggtggt gtgaaccgca ccggatacca aaccgtccac atcattttgc gccatcatca
     9421 tcgtaccgag taccacggtg tcttgcagtt gcttgcgcgc gtcttcgggt gtcaggcctt
     9481 tggatttgcg cagttcgcac atcggtccga cgtattgttc gaccaatgag gcgggatcga
     9541 tgatttccaa agagtcgggc aggctgatgc cgcgttcttt ggcaacggct tcgacttctt
     9601 cgcgtttggc aagcaggacg cagcgggcaa tgcctttttc gtggcagatg gcggcggctt
     9661 ggacggtgcg gggttctgcg ccctcaggca ggacgatgcg tttgtcggct tggcgggcga
     9721 agtcgatcag gttgtagcgg aattgcgccg gcgacaggcg ttttgcttcg cggcctgcca
     9781 atacggatac gtctttcagc gcgtcgctcg aaccgaagaa ggtcaggccg gttttttcgg
     9841 ctgccgcttc ggcaacggag gctgccgcgc cgtccacgac aaaaccttcc aatacgcccg
     9901 gcgcggcggc gaagaactgt ttggcaaggt tcagccgatt tgccagttcg tcggcatcgg
     9961 cgttgtcgga acggacggcg aagacggctg ccgcgtcaag ggacaatgcc agttcgacgt
    10021 ttttgcctgc gaggtagatt ttgtcggcat cgggcgcgat gccttcgatg acgaggttgg
    10081 cggcatcgag tgcggcaact ttgccgacca gtgcgtcgaa ccagtcgtcg cttttgcctt
    10141 gcgcgagcag ggtttcggcg gttgcgtcaa cggcttggaa aatttgtgcg tccagtgctt
    10201 ttgcaaaggc ttgtgcggcg gcggaggcgt ccagtccggc agatacgggt acgatgagta
    10261 cttttgccat gatatatcct ttcgtatgct gcggtgtgcg gcatatgtgg ttggaagggg
    10321 cggcatatag gcagaaacgg ctgcctgcgt gccgtgcgtg ccgtgtttgg cttgaggcgc
    10381 gcaggttgaa tatagcaaac aaattctgtt tccaacaaga taaatatccg caggcttgtg
    10441 gatgctgccg cctttcagag ggtatttccg gggaagaaca gggcgggacc gtccaaatgg
    10501 aggacggcgg aaatgccgtc tgacagggtg ggggcggaag ggaggttgag cgtgaggacg
    10561 gtttgtccga cccggaggct gatttcggta tgccgcgctt tgggcgtggt tttgagaacc
    10621 acggcgtgaa tggaggcggt gggtgcggaa tgggggtgaa ggctgaactg ttccggacgg
    10681 atgagcagtg tgccgcgtgt gcctgcgggt gcgccgcttt gaacgggcag gcggcccaat
    10741 ccgcaatcgg cggtgccgtc ggcgttgagc gcggcgggga acacgatgcc ttcgccgata
    10801 aacagggcgg catcaaggtc ggcaggttgt cggtacaatt cgtgagggct tgcagtttgg
    10861 aggatgcgcc cctgtttcat cacggcaatc cggtcggcgt attgcagggc ttcttcgcgg
    10921 tcgtggctga cgaaaacggc agatttgccg ttggcgcgca gggcggcaat catgtcttcg
    10981 cgaatctggc ggcgcaactg ttcgtccagc gcgctgaagg gttcgtccaa caaaatcagt
    11041 tcgggatcgg gtgcgagggc gcgggcgagg gcgacgcgct gttgctgtcc gcccgaaagt
    11101 tcgtgcggat agcgtccggc aagttcggaa atgccggtca attccaacat agcttcgatg
    11161 cgctgccgct cttgcgccgt cttgcctttg ccgttgccca gcccgtaggc ggtgttgcgg
    11221 taaacggtca ggtgggggaa cagcacacct tcctgtacga cataacccaa acggcgttcg
    11281 cggacgggga ggttggtatt tttcgagaag atggttctgc cggaaagcga aatttcgcca
    11341 aaatcgggtt gttcaaaacc ggcaaggcag cgtaaaaggg tggttttgcc gcagccggac
    11401 gcgccgacga taaagaggat ttcgcccggg tcgaggctga gcgaaatgtc gtttaaaact
    11461 ggggtgtttt gaaaactttt ggacaggtgt ccgatgtgca gggcggcggt catggcggta
    11521 cttcctcaag ctgttatttg aaggcgtatt tcttcagcag gaatacgggg atgccggaaa
    11581 ataataccag catcagcgcg taaggggtgg cggcggcgta ttgtgcgtcc gatgtgtatt
    11641 cccaaacggc ggtggagagt gtgtggacat cgtcggtggt cagcagcagg gtggcggtca
    11701 gctctttcat cagtttgagg aagacgagtg cgaatgcggc ggtaatgccg ggcaggatgg
    11761 acggcagtac caacgtcctg aaaataaaga agtgtccgcg ccccaatgtt gcgccgacct
    11821 gttccatccc ttttgggagt tgttccaagg aagtcctcag ggtggtttgc gccatcggca
    11881 ggtaaagcat gaaataggca aggatgacga cgataaaggt ttggtaaacg gcaggggtgt
    11941 agttgatgct gaaataaacc aaggataggg cgataaccaa accggggacg gcgtgcagta
    12001 aaaacggcag cctgtctatc caaacggtta aaaaattgcg atagcgaacc gatgcccaaa
    12061 caaggggcaa ggcacataat atagtcaaaa tcgcacctaa agccgatacg cttaaggaac
    12121 ggataaaggc atcaaatacg gatacgagcg cgaatgtgcc ggaagtgccg accatcatcc
    12181 aatgtatcaa tacgccaaag gggataataa tgcccaaagt caacaagctg cttaaaaaaa
    12241 caatcgcgcc aatctgaccg ggcagtttga gggttttgac gggataagga cgggcaacgc
    12301 ctttgccgct gtggtaaatc ttggctttgc cgcgaaatat gctttctcca aatacgacga
    12361 tgccgcacac cgccattaaa acagcggaaa gcagggcggc ggtattgttg ttgtaggaca
    12421 tttcgtattc ttggaaaatg gcggtggtaa aagtggggta gttcaaaatg gataccgcgc
    12481 caaattcgac caacatatgc agggcaatca gtaacacgct gctgccgatg gcgggtttga
    12541 gctgggggag gatggcggaa aaaaaggttt gcaggcggct tttgcccaag gacaggctga
    12601 cttcttcgta agacaggctg atgcgtttga gtgccgcctc gacgggcagg taggcgagcg
    12661 ggaacgagga caggctcata atcatcactg tcccccaaaa gccttcgaca cggaaggtca
    12721 ggctgatcca ggtgaaacag ctgacaaatg cggggatgca caaaggcagg gtgattgccg
    12781 tctgaaaaaa ggttttgccg aagaagcggt aacgttggaa caaaagggcg caggcaatgc
    12841 ccaaaacaat ggaaatcagg gtaacgcccg ccatcatcgt caaggtgttg gagagcaaat
    12901 cccacatacg cgggcggaac aacagttcga cggcgcggtt gatgccgacc tgccacgaac
    12961 gcatagcgac atataaaaaa ggcagggtaa gcggtagggc aatcagtagg atgaggccgg
    13021 taagccaaat gggtattttt ttaggagaca tagtgttttt tatcggcaaa acgggcggac
    13081 agtataaatg tccacccgtt tgacaatccg aaaacggctt atttcatacc ggcttgctca
    13141 agcagccggg tggcgtgttc tttttcggaa acagtggtgg cggacacttg gggtgcttcc
    13201 aacttggcga tgggttccaa attgaaggtg gataccacgt gcggattcaa aggatactcg
    13261 gcacggacgg cggtcagggc gcgctgtcct tccttgctgg cgaggaaggc gacgaatttt
    13321 ttcgcctcat ccttgttttg ggaggatttt aacacggctg cgccggaata ggtaacgagt
    13381 gcgccgggat ctctgtggcg gacgaaattc aggcgggtgt ggacattttg tacgcctttt
    13441 tcacgcgcaa aagcgtgcca gtagtagttg ttgatgaggg cggcatcgat ttcgccgttt
    13501 tcaaccgctt gaagggcgac ggagttttta gcgtaaggct tgccgtattc tttcagacct
    13561 ttgagccatt tcaatgcggc cgcttcgcct ttcagtttga cgatggcgac aacctgttcc
    13621 aagaacgcgc cggaagtggg ggcgtaaccg atgcggtttt tccatttcgg cgtggcgtaa
    13681 ttcaggacgg atttttccaa atctttttca gacagtttgc gggtgtcgta aacgacgacg
    13741 cgcgaacgtc cgctcagtgc cacccagtct tttttggcgg caaccggcac gcccttgccg
    13801 cgtgtttcgt tgatggtgga ggcgggcagg ggctctagga ggttggcggc ggaaagggtg
    13861 gcgagtgccg ggatttgttc ggaatagaat acgtcggcgg ggcttcggct gccttcttct
    13921 ttgatttgac cggcaagctg gtcgcctttg gcgctgttga gtttgacttt gatgccggta
    13981 gcccgggtaa aggcatctgc aacggcttgt gctgcttctt tgtgttggcc gttgtacacg
    14041 gtaatgtctg ccagcgcggg ggttgcggcg gtcagggctg cggcaagcag tgcgtatcgg
    14101 atagatgttt tcatatcgat tttctcctaa tgaatgagag tgtacacctt gttaaaacat
    14161 aacggtgtgt agtgtattcc ttcttcttta taaatgcaaa taattatttt ttaaatttgt
    14221 tattatccga tccggttatg gtttgttctg acttgtattt tttccgtgca tcgcgcccgt
    14281 aaggcggaag cggcgggcaa tgcgtggcgg aatgtgggta aaggcggcat tttgatttgt
    14341 cggaatgctt gagaacccct ctctttaaaa cagcgtctga acagggttgc cggaatagta
    14401 ttgccatccc ggcagataca gtttgtcggg gtcttgccaa tattgttcat ccagactgtt
    14461 cggcagtgag gcggttttgt tgtcgagatg ttttgttata tcccacattt ctttcaggtt
    14521 tttaccttcc gattggaggt ggcggatttc ttttctcaat gcgtctgccg cccgatggat
    14581 cagcattgcg ctgtgtatcg cgccgatttt tttcagaacg gaacaagtct tttcggcggc
    14641 gggaaaaccc caattgcaga caaattgcag tatgccgccg ttaccgatat cggcttctgt
    14701 ccgccaaatc aaaaccagct cctgttcccg cccgtccatg ctttcgagtt tgccgtcatg
    14761 ctgttcaaaa agtttgtcca ccgcctgcca catcatctgt tcgaagcggt cggcttgggt
    14821 atccgtatcg gtcatcgtgt tcttgccttt taaaaatgcc gtctgaacat ttcttcagac
    14881 ggcatcgggg gttaagccaa catttcccgc cagcgtttga cttggaaacg gacttgttca
    14941 ggcgcggtgc cgcccaagtg gttgcgcgcg tttaagctgc cttcgggtgt cagcacgccg
    15001 taaacgtcgt cggcaatcaa atcgctgaaa ccttgtaaga cttcgagcgg cagttcgctc
    15061 aaatcgacgc cagcttcatc ggcgtggcgc acggcttggg cgacgacttc gtgggcatcg
    15121 cggaagggca tacctttttt aaccagataa tccgccaagt cggtggcggt ggcaaaaccc
    15181 tgcatcacgg cggcgcgcat attgtcgggt ttgacggtta cgccgcgcat catatcggcg
    15241 taaatccgca gcgtgtcgat aagcgtgtcg gcggtgtcaa acaaaggttc tttgtcttcc
    15301 tgattgtctt tgttgtacgc caagggctgc gatttcatca gggtaatcaa gccgataagg
    15361 tgtccgatga ctcggccgga tttgccgcgc acgagttcgg gcacgtcggg gtttttcttc
    15421 tgcggcatga tggacgaacc tgtgcagaaa cggtcggcga tgtcgataaa gccgaaacgc
    15481 ggactcatcc acaaaatcaa ttcttcagac aggcggctca ggtgaaccat aaccagcgag
    15541 gcggcggcgg tgaactcgac ggcaaaatcg cggtcggata cggcatcgag cgagttttgg
    15601 cagatttgtt caaagcccaa tagctcggcg gtaatttcgc gctgaatcgg gtaggtcgtc
    15661 ccggcaaggg cggctgcgcc gagcggcata cggttgacgc ggcggcggca gtccgccatc
    15721 cgttcgttat cgcggccgag catttcgacg taggcgagca tatggtgtcc gaagctgacg
    15781 ggctgggcga cttgcaggtg ggtaaagccg ggcataacgg tttcggcgtt ttgttccgcc
    15841 aaatccagca atgccgtctg aaggttttga atcaggcttt ggataacggt aatctggtca
    15901 cgcagccaca ggcggatgtc ggtggcgact tggtcgttgc ggctgcggcc ggtgtgcagg
    15961 cgtttgcccg cgtcgccgat tttgtcggtc aggcggcgtt cgatgttcat gtggacatct
    16021 tccaaatcgg acgaccattc gattttgccg ctgcggattt cttcgaggat ttccgccatg
    16081 ccccgacgga tgtccgccaa atcgccttcg tccaacacgc cggtttcttt caacatttgc
    16141 gcgtgtgcca gcgagccttg aatgtcccat tcggcaagcc gtcggtcgaa accgatggag
    16201 gcggtgtatt gtttgacgag ttcggaaacg ggttcgttga aacgtccgga ccaggttttg
    16261 tcgtgcataa ggattccttg atggggttat tcggtgcggt atttttccaa aaggcggcgg
    16321 aagggttcgc cggtttcggg gtgtttcagg ccgtaggcga cggtggcttc gaggtagccc
    16381 agtttgctgc cgcagtcgta gcgcgtgcct tcaaaggggt gcgccaggac aaattcgtga
    16441 tcgagcagct tggcgatgcc gtctgtaagc tggatttcgt tgcccgcgcc gcgcggcagt
    16501 ccggtcagta agtcgaaaat gcgcggggta aggatgtagc gtccgacgac ggcaaggttg
    16561 gagggcgcgt cttcgggctt gggtttttcg acaatgccga taatgcgttg gaactgtttg
    16621 agctgttcgg tttcgacgat gccgtatgag ccggtttgcg atgcttcaac ggtttctacg
    16681 cccaaaatgc tgttgccgct gcgcccgtac acttcgacca tttgtttgag cgcgcctttg
    16741 ggggcatcaa tcaggtcgtc ggcaaggata acggcaaagg gttcgtctcc gatggcggcg
    16801 cgggcgcaca agacggcgtg tcccaagccc agtgcttccg cctgacggat gtagaggcag
    16861 gtaatgttcg gcggcaggat gttgcggacg tgttccaaca atttgtcttt atggcgcatt
    16921 tccaactcgg tttcgagttc gtatgccttg tcgaaatggt cttcgatgct gcgtttgttg
    16981 cgtccggtaa caaacaccat ttccgtgcag ccggcttcca cggcttcttc tacggcgtat
    17041 tggatcagcg gcttgtcgac gatgggcagc atttctttcg ggcttgcctt ggtggcgggc
    17101 aggaagcgtg ttcccatccc tgcgacgggg aagacggctt tttttatcgg tttcattctt
    17161 tttcctttgt attgttttga tgtttaaagg gcgagtttgc ggattaaatc ggcgagtgcc
    17221 tgcgcgcggt ggctttcgcg gtttttgacc tccgaatcca attcggcggc ggttttgccg
    17281 tgtccgggca gataaaaata cgggtcgtaa ccgaaaccgt tttcaccggc gggaacgccg
    17341 ttccattgtc cgtgccatac gccctcggcg ataattggac gcgggtcgtc tttatggcgg
    17401 acaaaaacca atacgcagac gtagcagcag cttttgtcgg tcttgccggc aagttcggcg
    17461 gcaagtttca ggttgttggc ggtatcggat ttgggattgt cgcccgcgta acgtgcggaa
    17521 tggatgcccg gcgcgccgtt tagggcggcg gcacagatgc cgctgtcgtc ggcaagcgcg
    17581 ggcagcccgc tgtattcggc ggcatggcgt gcttttgcca gcgcgttttc gacaaaggtg
    17641 ggatagggtt cggggcattc gggtatgccg aatgcggatt gcggcaatac ggtgatgctg
    17701 taaggtttga ataagttgcc gaattcttcg agcttgccgg cattgccgct tgccaaaacg
    17761 attttttccg gtttttcaga catagcggtt ttcctttgcg gcggattggg cggcgcgtag
    17821 ggatttgtgc cgcaggtaga gggcgaggct gccgatttgt ccgaacagtg cgccgaatgc
    17881 gaaaagaaag gaggcgacgg cgaaggcttt gctgccgacg gtcagcagat aagcacccaa
    17941 gaggacgagc agcataaatg ccagtgtgaa gagggcaaca gacaacagat agatttttcg
    18001 gcggttcatg gcgttcggtc ggaaacggta tgttcggatt atagccgatt gggacggtat
    18061 tccctagagc ttggaaaaat gccgtctgaa acggcgttca gacggcatgg cgggcgttat
    18121 gcctgtttgt tccaacgttc gatggattct ttgatgactt ttttcgcttc ttccgcatcg
    18181 ccccacgatt cgactttggt cgtacctgct tttttcaggt ctttgtaatg gttgaagtgg
    18241 aactcgattt gtttgatcag ctgttgcggc aaatcggaca aagttttgta ggcgttgccg
    18301 ttgttgcggt cgtcggcagg aacgcagacg attttgtcgt ccacttcgcc gtcgtcaacg
    18361 aatttcatca cgccgataac gcgcgcttcc aagaatacgc cggttgccaa aggttgttcg
    18421 gtaacgagca gcacgtccaa ttcgtcgccg tcttcgtcca aagtttgggg aatgaagccg
    18481 tagttggtcg gtttggcgaa gatggcgggt tcgacgcggt cgagttggaa tgcggcgagt
    18541 ttgcggttcc actcgatttt gtggttgctg ccggcgggga tttcgttgac gacgttgatg
    18601 atgccgccgt ccacatcgcc gggggtcagg atttggttga agtctgccat ttggtttcct
    18661 ttgtttggga aagtttgaag tttgaaagta tagcacaaac gtccggctga aaatgcgccc
    18721 gatgccgtct gaaagggtgt acgggcgcgt gttaccgttt gcccaaaaac ctgcccagtt
    18781 ccagaatcgc gcgcctgttg gacggggaaa acactttttc cgccgcttct tccaagccga
    18841 accagccgta ggagacgtgt tcttcgggtt gcagggcgat gggcgtatca cgcgggattt
    18901 cggcagagaa gaggtgttcg cggttttcaa acacgccttt tggatagcgg tgccgccagt
    18961 ggtggtagat ttcgtaaacc gtgctgtcgt gccagtcttg aagctgcccg tccgccagca
    19021 ggatgccggt ttcttcccaa acttcgcgcc ttgccgtttg ggcgacggtt tcgcccggtt
    19081 cgaggctgcc ggttaccgac tgccaaaatc cttccggatg cgtgcgttcg atgagcagga
    19141 tgccgccgtc cccgctataa aggacgacca gtgcggagac ggggtatttg agcggttttg
    19201 ccatcggcat ctttcggcgg gctgcggtaa tgaaggggcg gattatagca aacgccgcac
    19261 gttatggcgt ttatcctttt ccgtatcctt ttttctgcac ggatgggacg cgccggtgtt
    19321 tgccggtaaa ttttccgttg tgtcaaaaag ataagggcgg ttgtgatttt aatgcttgcc
    19381 aaagcgtcgg gcggaaacta taatccgaaa cttatcgagt cggagtgtgg cgcagtctgg
    19441 tagcgcactt gcatggggtg caaggggtcg aaggttcgaa tcctttcact ccgaccaaaa
    19501 attccgaaag ccgctttcaa aagcggcttt tttgccgtcc gtatgattat gatgtagagt
    19561 acgcggcgac agacattcaa atgccgtctg aaaaccgttc agatggcatc tctttatctt
    19621 agtttcattc cgtaccatct taaggaacat caaattgggc atttcccgca aaatatccct
    19681 tattctgtcc atactggcag tgtgcctgcc gatgcatgca cacgcctcag atttggcaaa
    19741 cgattctttt atccggcagg ttctcgaccg tcagcatttc gaacccgacg ggaaatacca
    19801 cctattcggc agcagggggg aacttgccga gcgcagcggt catatcggat tgggaaacat
    19861 acaaagccat cagttgggca acctgttcat ccagcaggcg gccattaaag gaaatatcgg
    19921 ctacattgtc cgcttttccg atcacgggca cgaagtccat tcccccttcg acaaccatgc
    19981 ctcacattcc gattctgatg aagccggtag tcccgttgac ggattcagcc tttaccgcat
    20041 ccattgggac ggatacgaac accatcccgc cgacggctac gacgggccac agggcggcgg
    20101 ctatcccgct cccaaaggcg cgagggatat atacagctac gacataaaag gcgttgccca
    20161 aaatatccgc ctcaacctga ccgacaaccg cagcaccgga caacggcttg ccgaccgttt
    20221 ccacaatgcc ggcgctatgc tgacgcaagg agtaggcgac ggattcaaac gcgccacccg
    20281 atacagcccc gagctggaca gatcgggcaa tgccgccgaa gccttcaacg gcactgcaga
    20341 tatcgtcaaa aacatcatcg gcgcggcagg agaaattgtc ggcgcaggcg atgccgtgca
    20401 gggtataagc gaaggctcaa acattgctgt catgcacggc ttgggtctgc tttccaccga
    20461 aaacaagatg gcgcgcatca acgatttggc cgatatggcg caactcaaag actatgccgc
    20521 agcagccatc cgcgattggg cagtccaaaa ccccaatgcc gcacaaggca tagaagccgt
    20581 cagcaatatc tttacggcag tcatccccgt caaagggatt ggagctgttc ggggaaaata
    20641 cggcttgggc ggcatcacgg cacatcctgt caagcggtcg cagatgggcg cgatcgcatt
    20701 gccgaaaggg aaatccgccg tcagcaacaa ttttgccgat gcggcatacg ccaaataccc
    20761 gtccccttac cattcccgaa atatccgttc aaacttggag cagcgttacg gcaaagaaaa
    20821 catcacctcc tcaaccgtgc cgccgtcaaa cggcaaaaat gtcaaactgg cagaccaacg
    20881 ccacccgaag acaggcgtac cgtttgacgg taaagggttt ccgaattttg agaagcacgt
    20941 gaaatatgat acgaagctcg atattcaaga attatcgggg ggcggtatac ctaaggctaa
    21001 gcctgtgttt gatgcgaaac cgagatggga ggttgatagg aagcttaata aattgacaac
    21061 tcgtgagcag gtggagaaaa atgttcagga aataaggaac ggtaataaaa acagtaactt
    21121 tagccaacat gctcaactag agagggaaat taataaacta aaatctgccg atgaaattaa
    21181 ttttgcagat ggaatgggaa aatttaccga tagcatgaat gacaaggctt ttagtaggct
    21241 tgtgaaatca gttaaagaga atggcttcac aaatccagtt gtggagtacg ttgaaataaa
    21301 tggaaaagca tatatcgtaa gaggaaataa tagggttttt gctgcagaat accttggcag
    21361 gatacatgaa ttaaaattta aaaaagttga ctttcctgtt cctaatacta gttggaaaaa
    21421 tcctactgat gtcttgaatg aatcaggtaa tgttaagaga cctcgttata ggagtaaata
    21481 aaaatggcaa tttggaatta tcggtactac cttatcccat cagctgctat cagaaataaa
    21541 tttaatgcaa ataaagatat catacttgat gagtatcgat ctaatggttt tcagaatttt
    21601 aatgagaata aaagttttga aaattacttt atcgataatg atgttatatt attatcaata
    21661 ataaatgaag caaaaaaaca gcttaaattg aaagaatctt gggataaaga cgcaatcatg
    21721 ttttgtgata attttggtaa tagtcttacc gtttggccag atgatataga gtgcgaactt
    21781 gatttaagat ttgattatac taaatttatt cagaaaacca ttgattgggc aataaaatat
    21841 aattgtctac ttgtaataga aaaaacagga aatgtagttt cccctaatat aaataatctg
    21901 atgtatgaaa taaaagcata tttggaaagc aagccgtggc ccatatgaaa cctaaactca
    21961 acaagtagga tgtgtgcgga acgcacgtat gcggttctca aggtttgagc taagaggccg
    22021 tctgaaaaca gaaaaactgt ttcagacgac ctttctttta accagttgcc acagcaaccg
    22081 gacaaaagca gcctacctcc acatccatat aggcaataca ggggagatat tttgtaaatt
    22141 ctacgaatat tttacctgct aaacagggta ggatatggta tgaagcgaac attggcttaa
    22201 taaacactat gtcaagatcg aatcaggctg gtactagatt gttgtattcc aattatggat
    22261 tgctatatat aacaactgat cattatatct ctgcaacacg gtttgtagct tggaaatagg
    22321 agtataactt atgcaattag agattatcgg tagtaaaatt tatacggaac aagattttca
    22381 taatcaaatt tcaaaaatat tttctataca agattattat gggaacaatc ttgatgcttt
    22441 atgggattta ttaagcacaa atgtagaacg accgattact ttggtatgga aagatgctat
    22501 gttctcaaaa aatcaattag aaaatatatt tattgaaatc gtaaatgttc tagaaagagt
    22561 taagaaacaa gatgaggatt atggattcga agaaaaattt aattatattt tagagtaagt
    22621 aaaccctaat tacattacgt acacaggctt aaaactcccc agagccaatt aagcaagccg
    22681 taacccatat aaaacttaaa ctcaacaagt agcatgtgtg cggaacgtac gcatgcggtt
    22741 cttaaagttt gagctaagag gccgtctaaa aacagaaaaa ccgtttcaga cggtctttgt
    22801 ttaacgccac cgatccagcg ggttacaaag cgcagtcaat gccgctgcgc cttatgcctc
    22861 cgaagcaata ggcagaacat ttggacacgg tgaaaacaaa aacgaaaccg cccaagccgt
    22921 cggacatttc cttttaggag cagctattgc ccgcgtcaac ggtggtaatt ttgctgccgg
    22981 cggctcggca gcagttgcag ctgaaaaggc ggcggaacat cttgcccaac agtataacga
    23041 cggtaaaacc gcaatcgatc cgcaaacagg cgagttcaat gccaacctgc tgccggaaca
    23101 tatcaaagag gaaatcaaat caaagagcgg ggtgattgca tcgctgacgg gcgcggccgt
    23161 gggcggcacg ccggtagatg cgcaaaccgg aggtgcggtc ggacagaatg cggtggaaaa
    23221 caacctctat ctgacatcgg aagccttaaa gaaggacaag cagacagctc gtaaaattta
    23281 ttccgtcata aaagagcaag tcaagcatga atgcagttcc acaggaagaa ttaccgaatg
    23341 tcgtcaaaat ataggacgca ttatcgaatt tacccaagac aaacgctttg acagtaggtt
    23401 taaggactta aaaaaagaat ccttatatta cctaaataaa catcctgatt tagtagcctc
    23461 ttatttgaag gctgaatacg aaaagctgga tagggaagac aaaagtatcc tgcaccgcta
    23521 catctcaccc ggggctgaaa tcgtttcggg cagtttgggg gttgttcttt caggagtagc
    23581 cggaggcgga tcttgtgccg agactttcgg cttaggctgt gccgccgctt tggttggtgt
    23641 aacgtcttcc tacgatcatg tcattactgg gacgaaaaac ttcggaaaaa aagccagcga
    23701 gcaacgaccg acgattgcgg ttcaggcctt gaagcagttg gggctgtcgg agcaggctgc
    23761 ggaatatgtt cagttctcta tagatttgtt cagtgtgggt aaatcggggg gcggtatacc
    23821 taaggctaag cctgtgtttg atgcgaaacc gagatgggag gttgatagga agcttaataa
    23881 attgacaact cgtgagcagg tggagaaaaa tgttcaggaa acgagaagaa ggagtcagag
    23941 tagtcagttt aaagcccatg cgcaacgaga atgggaaaat aaaacagggt tagattttaa
    24001 tcattttata ggtggtgata tcaataagaa aggcacagta acaggagggc atagtctaac
    24061 ccgtggtgat gtacgggtga tacaacaaac ctcggcacct gataaacatg gggtttatca
    24121 agcgacagtg gaaattaaaa agcctgatgg aagttgggag gtgaaaacga aaaaaggtgg
    24181 gaaagtgatg accaagcaca ccatgttccc aaaagattgg gatgaggcta gaattagggc
    24241 tgaagttact tcggcttggg aaagtagaat aatgcttaag gataataaat ggcagggtac
    24301 aagtaaatcg ggtattaaaa tagaaggatt taccgaacct aatagaacag catatcccat
    24361 ttatgaatag taatatttat gaaaaattag gagattaatg atgaaaagaa ttaagtgctt
    24421 ttgtgataaa tttccatcag gagatacatt tagaatgtgt atcattctgg atgactatga
    24481 taatagggtt gattattatg taggaatata tgattacatt acgtctacct taatgagcga
    24541 tatttactat cgatccacga ttgatgagca tttcaagatt atagaattaa tagaaaataa
    24601 tccaaatgaa atttatgatg atggcggtgg tcaacaattt tgcctagaat ttcatcatga
    24661 taaggtcatt ttttatcaca atgaatttga tgaagaagat ggttatccag tattaagctg
    24721 ttcgctgcat acttttaaaa ctgctttaat tgcttggaat gcttttttgc aattgcctaa
    24781 aagtattcat tcggtggtgg agactgtgat tgaggaataa gcataattag cttaatgaat
    24841 agaatcagcg atatagaaag gtagaatcag atatatccca cctaaaaatt actctccttc
    24901 agcaccgcta ccaaaaggac ctaataatgg atatagtgcc gggaatttaa gcaaaaaagt
    24961 cctgacttta gtttacataa acggcaaact caaattcggg ccaagcagtc tgccgtcttc
    25021 ggcattgaaa gcacctgttc ccattgtcgg caaggctgag gctccgcccg gaaaagctgt
    25081 gcctgtcggc gaagcccgca ttccttcaaa tccaaaagcg gtaaaaggaa caaccgttac
    25141 cctgtcgcag gaacaacgcc tagccaacgg agagctgata ccgaaaggct ctgtggtaac
    25201 gtggtcggac aatgcggtga aggttgtgcg gcctgatggg aaaaagcgga gatttaatag
    25261 tcgggattgg gaacagaagg cattgccgaa accgcaacta ccgaaagatt acagagatga
    25321 catcaacata ggtggaaata atcctagatt taatcagcaa cggcatgaaa aaatcgaatc
    25381 ccacaaccca aacagaatag aggagatacg ccaacgtaat gccgtttctt ctgcagaaag
    25441 gcaggctgat gcattaaaaa ggatgcaaac gtctggccgc acccgcctgc ctgggcagcc
    25501 acaaaaagga aaacccggcg ctgctagaga aatgtcctct tcagacaatc cgaatcaaac
    25561 agccgaggct tttgcacgtc gatatttagg caggaaacct acactaatgg aacgagtcga
    25621 aggtgaaaaa ttatacggag gtaaatgtgt gggctgttgg aaagcgcaaa tgccggacgg
    25681 aacctttgtg gtgtataggc cagcaggtaa tgcttctatg accaagggaa ctactgcgtc
    25741 tgttgatatt aataacccaa cattaaataa cttaaatatt aatttaaaag gcaagccaac
    25801 agagttaaaa tttaaattcc ctgctagata aggatgtttt aatgaattta gagacagcag
    25861 aaaatattca atgcgaattt gagtatttag cattagatgg ttattttcca atgcattttg
    25921 catcgcatgg tcaaggaaac aaagattggc aatttgcagt tgaattcata tatagattat
    25981 taatatgtca attagcaact cttgaaccta tcaactttga aacgaaaaat gatattttag
    26041 acttttgcca taatttagca aaacaaagtc cttttaataa tgataatgag gtgtggtatc
    26101 agggggaaat tgtattaaca aaaaaaggga tagatttaat aaaggaatat attccagagg
    26161 cgtttgaaga gtggaatgga aagaaatttg aattaaacat tcctttcatt aaaacattaa
    26221 aaaatatatt tgttaattat gaggtagcat gggatgagaa taatccacta ttccccatta
    26281 tatctttaaa cctcggcacc tgataaacat ggggtttatc aagcgacagt ggaaattaaa
    26341 aagcctgatg gaagttggga aatgaaaaaa ggaaggggcg gaaaacctat gacaaatcat
    26401 tctatgttcc caaaagattg ggatgaggct agaattaggg ctgaagttac ttcggcttgg
    26461 gagaaaagaa aggaattaga aggtcaaaaa tggagaggta caagtaaatc tggtgttgaa
    26521 atagaaggat ttacggaacc taatcgaaca gcttatccac tttataatag taaatgaagt
    26581 attgattaaa aaggtgtttt taaatgtata aaataaaatg tttttatgat cagtttagtt
    26641 ctggtgaatt atttaactac tgccagtttt tagataattc ttctaatcaa aagataaata
    26701 cacgaggaac ggtgtatcaa tatattattt atgttttaac aggagacctt tatctacaaa
    26761 aagacataga tgaaaattta gaatttatcc atcaagcaga aaataatccc aatgaagttt
    26821 attcaggagg tggacaaggt ttttgctggg atatttctgc tgaaaaagta gttttctatc
    26881 acaacgaatt tgatgaagaa gacggctggc ctgatttaag ctgttcgctg catactttta
    26941 aaactgcttt aattgcttgg aatgcttttt tgcaattgcc taaaagtatt cattcggtgg
    27001 tggagactgt gattgaggaa taagcataat tagcttaatg aatagaatca gcgatataga
    27061 ttggactgca aatccacgct tatacgctgt gccatgatta acaggaagag agcaacttgg
    27121 atgggctagt agggatggta agcatttaaa tatatcaatt gatggaaaga ttacacacaa
    27181 atgactaatt taaaattaga tttctactct gaagttatta taaaagattc ttgccctaat
    27241 gatttgttag aaaatggtga gactattaaa ggaaaaaaag gagtggtatt aggtataagc
    27301 gaagaagatg gtataatcta tggctatact attttacttt ttgatataaa atattgtata
    27361 tatatagata agaaatatat tataccgact ggaaaaaaat tctctcgcga tgatttttat
    27421 tagctaattt aagtaattta agcaagtttt ttagatactg aaactggtaa ttatgtttat
    27481 gtaagcataa ggagttcaga ttgaaggatt ctacagggca aatgaggctg gcaaccaagg
    27541 atttggcgga agccattaaa cgaggagaag tacgtagttc tgcttttaca acaaagcaac
    27601 taaaggcaat cgaaaaagga gaatctaaaa taccggatta cacttggcat catcatcaag
    27661 acactggaag gatgcaattg attaatgaag acttgcatca tgataccggc catatcggtt
    27721 ggagagcgat gagtaaagga aagtaa
//
LOCUS       DAXHMX010000008        26452 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHMX010000008 DAXHMX010000000
VERSION     DAXHMX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.008"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(92..880)
                     /locus_tag="ACFM4S_000259"
     CDS             complement(92..880)
                     /locus_tag="ACFM4S_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6050018.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1625)
                     /gene="bioH"
                     /locus_tag="ACFM4S_000260"
     CDS             complement(882..1625)
                     /gene="bioH"
                     /locus_tag="ACFM4S_000260"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC6050019.1"
                     /translation="MPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTADEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            1624..2349
                     /locus_tag="ACFM4S_000261"
     CDS             1624..2349
                     /locus_tag="ACFM4S_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6050020.1"
                     /translation="MDFLSRWRRIADAPTIRRCVLCHGSSGVSDGICAGCRDDLAAYR
                     TDAANSCPLCFRHIQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRTLKHLADLGM
                     VRPLANLMVQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGSERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="ACFM4S_000262"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="ACFM4S_000262"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6050021.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="ACFM4S_000263"
     CDS             2889..3011
                     /locus_tag="ACFM4S_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6050022.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="ACFM4S_000264"
     CDS             3008..3727
                     /locus_tag="ACFM4S_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6050023.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            3789..4115
                     /locus_tag="ACFM4S_000265"
     CDS             3789..4115
                     /locus_tag="ACFM4S_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6050024.1"
                     /translation="MNIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4197..4781
                     /gene="ruvA"
                     /locus_tag="ACFM4S_000266"
     CDS             4197..4781
                     /gene="ruvA"
                     /locus_tag="ACFM4S_000266"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6050025.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            4781..4918
                     /locus_tag="ACFM4S_000267"
     CDS             4781..4918
                     /locus_tag="ACFM4S_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050026.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4944..6800
                     /locus_tag="ACFM4S_000268"
     CDS             4944..6800
                     /locus_tag="ACFM4S_000268"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6050027.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VNDGMATLSKPHTILDKPRALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6805..7728
                     /gene="rsgA"
                     /locus_tag="ACFM4S_000269"
     CDS             6805..7728
                     /gene="rsgA"
                     /locus_tag="ACFM4S_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6050028.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            7752..7841
                     /locus_tag="ACFM4S_000270"
                     /pseudo
     CDS             7752..7841
                     /locus_tag="ACFM4S_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            7950..8213
                     /locus_tag="ACFM4S_000271"
     CDS             7950..8213
                     /locus_tag="ACFM4S_000271"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6050029.1"
                     /translation="MSKWRGFNRKGNTMKKNAPKSFEEALSRLESLTQAMQGEMPLED
                     ALAAYQEGNELVRYCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            8197..9093
                     /locus_tag="ACFM4S_000272"
     CDS             8197..9093
                     /locus_tag="ACFM4S_000272"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6050030.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9202..9501)
                     /locus_tag="ACFM4S_000273"
     CDS             complement(9202..9501)
                     /locus_tag="ACFM4S_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6050031.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAILAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9613..11058)
                     /gene="nuoN"
                     /locus_tag="ACFM4S_000274"
     CDS             complement(9613..11058)
                     /gene="nuoN"
                     /locus_tag="ACFM4S_000274"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6050032.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPAAGGNNAAKF
                     LLSINALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11068..12564)
                     /locus_tag="ACFM4S_000275"
     CDS             complement(11068..12564)
                     /locus_tag="ACFM4S_000275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6050033.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVNGQLNDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFTILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(12653..14689)
                     /gene="nuoL"
                     /locus_tag="ACFM4S_000276"
     CDS             complement(12653..14689)
                     /gene="nuoL"
                     /locus_tag="ACFM4S_000276"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6050034.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIG
                     YVAIEPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVL
                     SAWLLYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDT
                     AIIDNGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(14738..15079)
                     /locus_tag="ACFM4S_000277"
     CDS             complement(14738..15079)
                     /locus_tag="ACFM4S_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050035.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(15203..15778)
                     /gene="fic"
                     /locus_tag="ACFM4S_000278"
     CDS             complement(15203..15778)
                     /gene="fic"
                     /locus_tag="ACFM4S_000278"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6050036.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKIVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(15812..16117)
                     /gene="nuoK"
                     /locus_tag="ACFM4S_000279"
     CDS             complement(15812..16117)
                     /gene="nuoK"
                     /locus_tag="ACFM4S_000279"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6050037.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(16114..16785)
                     /locus_tag="ACFM4S_000280"
     CDS             complement(16114..16785)
                     /locus_tag="ACFM4S_000280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6050038.1"
                     /translation="MTFSAILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVYRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(16797..17276)
                     /gene="nuoI"
                     /locus_tag="ACFM4S_000281"
     CDS             complement(16797..17276)
                     /gene="nuoI"
                     /locus_tag="ACFM4S_000281"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6050039.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(17357..18433)
                     /gene="nuoH"
                     /locus_tag="ACFM4S_000282"
     CDS             complement(17357..18433)
                     /gene="nuoH"
                     /locus_tag="ACFM4S_000282"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6050040.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            complement(18436..20697)
                     /gene="nuoG"
                     /locus_tag="ACFM4S_000283"
     CDS             complement(18436..20697)
                     /gene="nuoG"
                     /locus_tag="ACFM4S_000283"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6050041.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLFQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQVANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDMVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLQGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQATVH
                     AAVPAARVNPNTLGRLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(20987..21424)
                     /locus_tag="ACFM4S_000284"
     CDS             complement(20987..21424)
                     /locus_tag="ACFM4S_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050042.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            complement(21424..22725)
                     /gene="nuoF"
                     /locus_tag="ACFM4S_000285"
     CDS             complement(21424..22725)
                     /gene="nuoF"
                     /locus_tag="ACFM4S_000285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6050043.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(23115..23588)
                     /gene="nuoE"
                     /locus_tag="ACFM4S_000286"
     CDS             complement(23115..23588)
                     /gene="nuoE"
                     /locus_tag="ACFM4S_000286"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6050044.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGISYGETTPDGKFTLVEGECMGACGDAPVMLLNNHKMCSFMTEEAIEKKLAELE"
     gene            complement(23588..24844)
                     /gene="nuoD"
                     /locus_tag="ACFM4S_000287"
     CDS             complement(23588..24844)
                     /gene="nuoD"
                     /locus_tag="ACFM4S_000287"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6050045.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(24834..25427)
                     /locus_tag="ACFM4S_000288"
     CDS             complement(24834..25427)
                     /locus_tag="ACFM4S_000288"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6050046.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(25440..25922)
                     /locus_tag="ACFM4S_000289"
     CDS             complement(25440..25922)
                     /locus_tag="ACFM4S_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6050047.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(25913..26269)
                     /locus_tag="ACFM4S_000290"
     CDS             complement(25913..26269)
                     /locus_tag="ACFM4S_000290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6050048.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         6576 a         7433 c         6720 g         5723 t
ORIGIN      
        1 cactatacat cggcatttcc tttagttctg tcggttttca caaacaaatg ccgtctgaaa
       61 cttggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttaa cggcgtgtcc gtacactgtt tccagcgtaa
      181 tactcaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacga catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac ggggtcgtaa aacccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cgggaaacat cacgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttcgg taaaagtgtc gcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtctcc agcgcgcgcg cccagttgtg cagcacagga aggatttgtt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgt tgcaccacgc ccttacccgt aaacctttgc caaaaaccgc
      661 ccttgcgcac ggctgcggca gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atatcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcagccg ttggtcggca tgttcggcaa
      841 gatggcggtg aacctgccag catttgtcct gcggattcat gtcaaacccc ttcgacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct gcctttcaaa cggcggtgca gatactcgcc catgcgcggc
     1021 ggcgtaatcg cgtctttgct accgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaaagctt cctgtaaggc ggagggcgtg
     1141 ccgcagcgcg ccaaatcggg caggattctg cctatgattc cgtccgcatc aggcgtgtgc
     1201 agaagctgga gttgtagaaa ctgtttgata tgtttggcat aatccgaacg gaacgcgccg
     1261 accattttgc ccaatgcagg cgcggcaagc ccttcgggat agtcttcgtc agccgtcagt
     1321 cgggcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggcgggcgt atcgatttgt
     1441 gcggcaacag cgtcggcggc tgcctcaata tcgaagggtt gcgcgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccggc cacgttgcag gcaggcgcgg catcaaatcg
     1561 tcgaacatgt ggcggttcgc cccccaaccg tgtatcaggt aaactttttt gacggcatca
     1621 ggcatggatt ttctctctcg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgccggctg ccgcgacgat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcag gcacatccaa
     1801 ggcggcgcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaac atgatacgta cgctgaaaca cttggcagat
     1921 ttgggcatgg ttcgcccatt ggcaaacctg atggtacaaa acccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ttgttggcac aacgctacgg ctggcagata
     2101 ctgccccgac acaccgtatt ccgacaccac cgcccgccgc aaagcacgct caaaggcagc
     2161 gaacggcggc gaaacatcaa aaacgccttt gaaatccgca caccgatacc ggaaaactgt
     2221 aatattctgt taatcgacga tgtctttacc accggcgcga cgctggacga actggcaaag
     2281 acgctgaaaa aatcgggcgc gaaccgcatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccagccg gaaatcccgc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg aattcgccag
     2581 cctgacggtg cacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcgccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc ccatgcagcc cggcagtcgg agtatgaacc tttccaatac
     2821 cgttgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaagttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acggcatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caaccgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaata ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcaac caagggacgg cacacgtcaa aatcgaacaa atcgtcccgg gccaatccgc
     3481 accggttgcc gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gtttcgtcat cgggtacgaa
     3601 cctctcggtt gaaaaacgcc gttacgaata cgtcgtcaaa atgggaccgt ttgcctcgca
     3661 ggaacgcgcc gccgaagccg aagctcaggc acgcggtatg gttcgggcgg tattgaccgc
     3721 cggctgacgg ttatttgata ccttattaat atccacattt tccaacccac gatttacaaa
     3781 ggcaaaatat gaacatcaaa caccttctct tgacctccgc cgcaaccgca ctgctgagca
     3841 tttccgcccc cgcgctcgcc caccacgacg gacacggcga tgacgaccac ggacacgccg
     3901 cacaccaaca caacaaacaa gacaaaatca tcagccgcgc ccaagccgaa aaagcagcgt
     3961 tggcgcgtgt cggcggcaaa atcaccgaca tcgatctcga acacgacaac ggccgtccgc
     4021 actatgatgt cgaaatcgtc aaaaacggac aggaatacaa agtcgttgtc gatgcccgta
     4081 ccggccgcgt gatttcctcc cgccgcgacg actgaatttg atacaatccg tgccgtctga
     4141 agcccgaacc ggtttcagac ggcattttgc acccgacact tcaggattcg gcacacatga
     4201 tcagcagact gaccggcaaa ctggttgaaa aaaaccctcc gcaaatcgtc atcgatgtca
     4261 acggtgtcgg ctatgaggcc gacgtatcga tgcagacctt ctacaacctg ccgcccgtgg
     4321 gtgaaagcgt acaactgttt acccagctta tcattcggga agacgcacat cttttatttg
     4381 gttttgccac tgcggaagaa cgcaagacct tccgccaact gattaaggtc ggcggcatcg
     4441 gcgcgaaaac ggctttgggc attttgtcag cgatgacggc agacgagctg gcgcgggcgg
     4501 ttgccgaaga agatgtcaaa cgcctgtctt ccgcgccggg aatcggcaaa aaaaccgccg
     4561 aacgtatggt cttggaactg cgcggcaagc tggtcgcgca tacggtaacg gacgggctgt
     4621 ttgccgccgc acccgccgcc gacgaaacgg aagacatcgt cagcacgctg cttgcgctgg
     4681 gttacagcga acgcgaagca aaagcggcgg tcaaaggcgt tccggagggg acggacgtgg
     4741 gcgaaggcgt gcgccttgct ttgaaaaacc tgctgaaata atgccgtctg aaggcggcgc
     4801 ggcgtttgcc ctgacgaaac cctgcgtttc cgcctcgcgc tgtcttcccg acggcttttc
     4861 ccgtaaaatg gcgttattgt ccttcctttc agacggcatt gtgtttgatg ccgtctgaaa
     4921 ctgttttacc gaaatcgaaa acaatgttga acaaaatatt ttcctggttc gagtcccgaa
     4981 tcgaccctta tcccgaagcc gccccgaaaa cgccagaaaa aggcttgtgg cggtttgtct
     5041 ggagcagcat ggccggcgtg cggaaatgga tagccgccct ggctgcgctg accgccggca
     5101 tcggcattat ggaagccctg gtttttcaat ttatgggcaa aatcgtggag tggctcggca
     5161 aatacgcgcc cgccgaactg tttgccgaaa aaggttggga actggcggca atggcggcga
     5221 tgatggtatt ttcggttgcg tgggcgtttg ccgcgtccaa cgtgcgcctg caaacccttc
     5281 agggcgtgtt ccccatgcgc ctgcgctgga acttccaccg cctgatgctg aaccaaagcc
     5341 tcggttttta tcaggacgaa tttgccggac gcgtgtccgc caaagtcatg cagaccgcgc
     5401 tggcgttgcg cgacgcggtg atgacggttg ccgatatggt cgtttatgtg tcggtgtatt
     5461 tcattacctc cggcgtgatt ctcgcctcgc tcgactcatg gctgctgctg ccctttatcg
     5521 gctggattgt cggtttcgct tcggtgatgc gcctgctgat tcccaaattg gggcaaaccg
     5581 ccgcatggca ggcggatgcc cgctcgctga tgaccggccg cattaccgat gcctattcca
     5641 atatcgccac cgtcaaactc ttctcccacg gcgcgcgtga agccgcctat gccaagcagt
     5701 cgatggaaga atttatggtt acggtgcgcg cccaaatgcg gctggcgacg ctgctgcatt
     5761 cgtgcagctt catcgtcaac acctccctga ccctctccac cgccgcactg ggcatctggc
     5821 tttggcacaa cggacaggtc ggcgtgggcg cggtcgccac cgccaccgcg atggcgttgc
     5881 gcgtcaacgg tttgtcgcaa tacatcatgt gggaatccgc gcggctgttt gaaaacatcg
     5941 gcaccgtcaa cgacggtatg gcaaccctgt ccaaaccgca caccatcctc gacaagcccc
     6001 gggcactgcc gctgaacgtg ccgcaaggcg caatcaaatt tgaacacatc gatttctgct
     6061 acgaagcggg caaaccgctg ctcaacggct tcaacctcac catccgcccg ggcgaaaaag
     6121 tcggcttgat cggacgcagc ggcgcgggca aatccaccat cgtcaacctg cttttgcgct
     6181 tctacgaacc gcaaagcggc acggtttcga tcgacgggca ggacataagc ggcgttaccc
     6241 aagaatcttt acgcgcccaa atcggtttgg tcacgcaaga tacctcgctg ctgcaccgtt
     6301 ccgtgcgcga caacattatt tacggccgcc ccgacgcgac cgatgccgaa atggtttctg
     6361 ccgccgaacg cgccgaagcc gccggcttca tccccgacct ttccgatgcc aaagggcggc
     6421 gcggctacga cgcacacgtc ggcgaacgcg gcgtgaaact ctccggcggt caacgccagc
     6481 gcatcgccat cgcccgcgtg atgctcaaag acgcaccgat tcttcttttg gacgaagcca
     6541 ccagcgcgct cgattccgaa gtcgaagccg ccatccaaga aagcctcgac aaaatgatgg
     6601 acggcaaaac cgtcatcgcc atcgcccacc gcctctccac catcgccgca atggacaggc
     6661 tcgtcgtcct cgacaaaggc cgcatcatcg aagaaggcac gcacgccgaa ctcctcgaaa
     6721 aacgcgggct ttacgccaaa ctctgggcgc accagagcgg cggcttcctc aacgaacacg
     6781 tcgagtggca gcacgactga accgatgccg tccgaacacc cgttttcaga cggcatttcc
     6841 acacccaacc ccaaagaaac catgaacgac accgcccaaa ttaccgccag ctacggccgc
     6901 cgctacatcg tgcgcacgcc cgacggcaca acctacgaag ccagcacgcg caaaaaacgc
     6961 gtcgatttcg cctgcggcga ccgtgtccgc atcagccccg tcaacgccga acaagttgtg
     7021 attgaagatt ttttaccgcg ccaaagcctg ctctaccgcc aagacgcgtg gaaaaccaaa
     7081 ctcattgccg ccaacgttac ccaactcctc atcgtaaccg ccgccgtccc gagtccgagc
     7141 gtgcggctgc tacagcgcgc cctgcttgcc gccgaagccg ccggcatccg cgccgtcatc
     7201 gtcctgaaca aagccgacct gcccgaaacc gccctttggc gcgaaaaact caaattctac
     7261 gaaacgctgg gttatcccgt catcgaaacc cgcgcactgg aaaacgccgg cagcctgcgc
     7321 cccgccctgc aagggcacag caacatcctg ctcgggcaga gcggtatggg caaatccacc
     7381 ctgaccaacg cccttttggg cagccaaacc gcccgcaccg gcgacatttc cgccgcactc
     7441 gactcgggaa aacacaccac cacccacgcc cggctttatg atttgaacgg cgaaacccaa
     7501 ctcatcgact ccccgggttt gcaagaattt ggtttacacc acctccaagc cgccgatttg
     7561 ccgcgctatt tccccgattt ccgccacctt gtcgggcaat gccgcttcca caactgcacc
     7621 caccgcgccg aacccggctg cgccttcaaa gccgccgcgc aaaccggggc ggcaagcccc
     7681 gaacgcctcg cctttttgca gggcatcacc gacgaactgc ccgggtaacg ccttgccgcc
     7741 tgggcggaaa aatgccgtct gaagccggat tcgggtttca gacggcatcc gtttttcaaa
     7801 aatgctacaa tccgcttttt actggaacat ccgaaactat gttccaacac gcaggacgac
     7861 acttaaagca ccgccctatg tgtcgtcctg atttggaagg gcttacaccc ctcccaaata
     7921 aagtctgatt ctaccgcccc gaaggacaga tgtccaagtg gcggggtttc aaccgaaaag
     7981 gaaacacgat gaagaaaaac gccccgaaat cgtttgaaga agccttgtcg cgccttgaat
     8041 cgctgacaca agctatgcag ggcgaaatgc ccttggaaga cgcgcttgcc gcctatcagg
     8101 aaggcaacga gctggtcaga tactgccaaa ccaagctggc gcaagtcgaa cagaaattac
     8161 aggttttgga cgcggacggg ccgaaggagt tgaaccttga atccgacgaa tgatttgaaa
     8221 gcgtggcaac agagggcgca ggcgcaaaca gagctgctgc tggaacgctt tttgccgtct
     8281 gaaaacgaaa taccgcacac actgcacgaa gccatgcgtt atgcggtttt ggatgggggc
     8341 aaacgtctgc ggccgatgct ggttctggca gcttcggaat taggcgaagc cgtgcatgaa
     8401 gcagtagaac aggcaatggc ggcaatcgaa atgatccatg cctattcgtt ggttcacgac
     8461 gatatgccgg caatggacaa cgacagcctg cgtcggggca aaccgacctg ccacatcaaa
     8521 tatggcgaag ccaccgcgct actggtcggc gacgctttgc aaacccaagc cttcgacgtg
     8581 ttaagccgtc cgacagaact gcccgcagca cgccagttgg caatgttgtc ggtgttggcg
     8641 aaagcgggcg gcagcgcggg catggcgggc ggtcaggcaa tcgatttggc gaatgtcggc
     8701 aaacaaatgg ttcaagccga tttggaacag atgcacagcc tgaaaacggg tgcgctaatc
     8761 cgtgcggcgg ttttattggg ggcgatgtcc tgcccgaatc tgtctgaaga cagtctggcg
     8821 gtattggacg attatgcggc aaaactgggg ctggcgttcc aagtcattga cgatgtgttg
     8881 gattgtgaag cggacacggc gactttgggc aagacggcgg gcaaagatgc agacaacgac
     8941 aagccgactt atgtgaaact gatgggcttg gaagcggcgc gctcatacgc acacaaactg
     9001 gttgccgaag cggtcgcgct gctcgaaccc ttcggcgaca aagccctgcg cctgcggcag
     9061 ttggcagaat ttgcagtcgc ccgcaaatat taaaaccggc gtatatcgcc ggctgaaacg
     9121 tgccggcaca gcgatgccgg cctgcacttg aatgaaacaa atgccgtctg aacaggtttc
     9181 agacggcatt tctatgcttg tctattccct gttgcgcgtg tgccaaaaat accgccacac
     9241 aaaacatgga aacgcaaaaa tcaggtacag gcagacgact gtggcataaa actcccaacc
     9301 ctgatcgtgt accgatcctg cacgggattc gagaatatag gcaagaatgg cggtcagtgc
     9361 gaaacctgcc gccagctcga tcatgtggtg tccgaaatgt ttgcgcttga gcgcggccac
     9421 gccgaacagt ctggtggtga ggaagggggc gttggcaaag atgagtgcca agaccaaaag
     9481 gatgtacatg gatgcggtca tggtgggctt tctgtttggg cattgtcggc ggcgatgccg
     9541 tctgaactgc attgcggaac ggcatatctt aacaaaaacg gcagcctctg acacggctgc
     9601 cgttgcggcg gcttacagcg tgttctccaa cgccttggcg caccagtcga taacggtttg
     9661 cggcataatg ccccacagca ccaacagcag cgcgttgatg cttaagagga attttgcggc
     9721 attgttgccg ccggcggcgg gacgggcgcg gccggattcg tcaaaataca tgactttgac
     9781 cacgcgcagg tagtagaacg cgccaatcag cgacatcacg acggcaaata cggacaacca
     9841 aacatagcct tgttttaaga gtgccataat cacgccgaat ttggcgtaaa agcccatcag
     9901 cggcggaatg cccgccatag agaacataac cagcagcatc aaaaaggcaa gccatacgcg
     9961 gtgttggttc aaccctgcca aatcgctgat gttttcgcac tcgttgtccc cgtccgacaa
    10021 caccatcaac acaccgaacc ctgccgccgc catcagcgcg taggtaatgg cgtaatagag
    10081 acccgccgca aagccgaccg cgcccgccat aaacgccagc aggataaacc ccatatgcga
    10141 tacggtggaa taggcgagca tacgtttgat attggtctgc atgatggcgg caaggttgcc
    10201 gaccagcagc gaggcggcgg caagcagggc aaacatcaga gaccagtcat gatgcacggt
    10261 tcccaacccg gtaacgagga tgcggaaagc gaaaacaacg gcggcgattt tcggggcagt
    10321 gccgaccagg gcggtaacag aagtgggcgc gccgtgatac acgtcgggca cccacatatg
    10381 gaacggcacc gcgccgagtt tgaacgcaac ggcgacgacg ataaacacca gacccagttt
    10441 caacagccat tcgtttgctt cttcattgaa ggaagaggcg agcacgccgg caaattccag
    10501 cgaaccggtt gcgccgtaaa ccatagaaat accgtagagc agcaggccgg atgccagcgc
    10561 acccagaaca aaatatttca aggcggcttc ggcggcaaag ccggaatcgc ggcgcagggc
    10621 aatcagggcg taaagggcaa gcgacaagag ttccaaaccg atataggcag ttaaaaaatg
    10681 ccccgcgctc accatcacgc ccatacccaa gagggcaaac agcgacaggg tgtaaaactc
    10741 gcctttgaaa ataccgcgca cttggttgta gggcttggca tagacaaaca gggtaaacat
    10801 caaggcatat aaaaccattt ttgccaaacg cgacataccg tctgcaatat acatcccgtt
    10861 gaacgaagac gtgctgccct gttcccacac cgccaactgc accacagccg taaccgccac
    10921 cgttgccaac gcgccgtaat gcgtccaccg gcgtttgtca tcactgaccc acaagtccgc
    10981 cagcaacaat aacaccagca gcgcaagcag cacgatttcg ggcatggcgg gcattaaatt
    11041 caaatcagac cagttcattt acacacctca aatcttgctt tgtgccacat gggcaatcaa
    11101 atcgtttgcc gcctgatgca ccacttcgat aaatgcgttc ggatacaggc ccatacccaa
    11161 taccgcaacc gccaaaaccg ccagaatcgt aaactcgcga cagttgatgt ctttcatttc
    11221 ggcaacgtgc ggattgtgaa tcgcgccgaa aatcacgcgc ttgtacatcc acagggtata
    11281 agacgcaccg taaatcagcg tcatagccgc caacgcgccc acccagaaat tcactttaac
    11341 cgcgcccata atcaccataa actcgcccac gaagccagaa gtcgccggca gaccggcatt
    11401 ggccataccg aacaacatca taaacgccgc aaacttaggc atcacattga ccacgccgcc
    11461 ataatcggca atattgcggg tatgcaggcg gtcgtacatc acgccgatac acataaacat
    11521 cgcggcagac acaaaaccgt gggaaatcat ttgaatgatt gcgcctttca atgcccaatc
    11581 attcaattgg ccgttcacaa acaggaacat acccaaagtg acaaaaccca tatggctgat
    11641 ggacgaatac gccaccagtt ttttcatatc ggtttgcacc aaagccacca taccgatgta
    11701 aatcacggca atcaggctca atacaatgat cacaggggca aaatagcgtg ccgcatccgg
    11761 cataatcggc aggataaagc gcaagaaacc atacgcaccc agcttcagcg taatcgccgc
    11821 caacaccatc gaaccgccgg tcggcgcttc aacgtgggca tccggcaacc aagtgtgcac
    11881 agggaacatc ggcactttta cggcaaatga caggaagaac gctgcaaata aaagctgttg
    11941 tacgcccaac ggaatctgtt tgatgttttg gaaatcgaca atagagaagc tgcctgtttg
    12001 atagtacagg taaaccatag caaccagcat caggagcgaa cccatcagcg tatagaggaa
    12061 cagcttgacc gacgcataga cgcggcgcgg gccgccccat acaccgataa tcaggtacag
    12121 cggaatcagc ataccctcga agaacacata aaacagaatc gcatcctgcg cggcaaacgc
    12181 accgttaatc aaacccgaca tcatcaggaa tgccgccata tactgcgccg ggcgtttctg
    12241 aataacttcc caaccggcca ataccaccag cagcgtaata aacgcattca agatgataaa
    12301 gagcactgaa ataccgtcca cgcccaatgc gtagttgatt ttcagaagcg gaatccactc
    12361 gtggaactcg gtaaattgat agccgccgct caaacggtcg aaaccggtaa acaggggcag
    12421 cgttaccaag aaaccggcaa gcgcacccat aaaggcaagc acacgcgcca gcggcgcacg
    12481 gctgtcctta ccggtcgaca aaaccagcac acctgcggcg atgggtatcc atattgccaa
    12541 gctgagtagg tagttggaaa acatagtggt taacctgtgg ttaaaataaa aatatcgttg
    12601 tacgggttgg tttgtcagac gacgttttaa gagaccgtct gaaacctcat ccttatctga
    12661 acaatcccca gaaggtcata ccgagcaaga ccaatacgcc gaacaccata gcggcggcgt
    12721 aggtatagat aaagccggtt tgagctttac gcacttgcgc ggcaatcgcg ccgaccagtt
    12781 tggcagagcc gttgacgatg ccgttgtcga taatggcggt atcgccgact ttccagaaga
    12841 aagtacccaa tgcgcgtgtg cctttggcga aaacgttgaa atacagggcg tcgaggtagt
    12901 atttgttttc aaacaaaacg taaatcggac ggaacgtctg tgcaattttc gctggcaggt
    12961 gcggcagttt gacgtacaaa agccatgcgc tcaacacgcc tgcgatagca aggtagagta
    13021 caggcgaatg caggctgtgc gacaccattg ccaatgcgcc gtggaactct tccttcatga
    13081 tgtgcatggt cggatgcgcg tcggcgttga cgaaaatcac atctttgaag aaatcgccgt
    13141 aaagcatagg ctcgatggca acgtagccga tgatgacgga cggaatggca agcaaaatca
    13201 aaggcagggt aacaaccaac gggctttcgt gcggattgtc gtttttgccc aagctgtgat
    13261 gttcttcgcc atgatgatcg ccatgatgat cgtcatgatg ttcaggcaag ctgcgccatt
    13321 tctcttcgcc gtggaacacc ataaagtatt ggcggaacgc gtagaacgcg gtaacgaaca
    13381 cgctggcaag gacggcaaaa taggcaaagc cgctgcccgg cagggtgctg tatttcgccg
    13441 cttcgataat cgaatcttta gaatagaagc cggagaagaa cggcgtaccg atcagcgaca
    13501 agttaccgat cagcatggtc agccaagtaa tcggcatgta ttttttcagg ttgcccatgt
    13561 ggcgcatgtc ttggtcgtgg tgcataccga taatcgcgct gcctgccgcc aagaacaaca
    13621 aggctttaaa gaaagcgtgg gtcatcacat ggaacatcgc cacggaatag gcagacgcac
    13681 ccaaagccac ggtcatatag cccagttgcg acaaggtgga atacgcaacc acacgtttga
    13741 tgtcgttttg aatcacgccc aagaaaccca taaacagggc ggtaatcgca ccgatcacca
    13801 taatgacaga cagcgcagtg ctgctcatct cataaatcgg cgacatacgc gataccataa
    13861 acagaccggc ggtaaccatc gtcgcagcgt gaatcaatgc agaaatcggg gtcgggcctt
    13921 ccatcgaatc aggcagccaa acgtgcagtg ggaattgtgc cgatttaccc atcgcaccga
    13981 caaacaggag caaacaggtg acagtaatca acgaccattc cacaccgggg aaaagctgga
    14041 tggtggcatt ttgcacgttg ggcagataag cgaatacatc ttgatagcgc aggctgccgc
    14101 cgaaataggc aagcaccaag ccgataccga gcaaaaagcc gaagtcgccg acacggttga
    14161 tcaaaaaggc tttcaggttg gcaaatgttg cgcttggacg tttgaaatag aaaccgatca
    14221 agagatacga caccaagccc acagcctccc agccgaagaa gagctggatg aagttgttgc
    14281 tcataatcag catcaacatg ctgaaagtaa acaaagaaat atagctgaag aagcgttggt
    14341 agccgacttt ttcatcgtgc atatagccga tggtgtagat atgcaccatc aacgatacgc
    14401 ccgttaccac gaccatcatc atcgccgtca tcgtatcgac caagaagccg acggagaaat
    14461 ccaaaccgcc catcgtcagc caggtataga cgttctcatc aaacttggca cggctgccat
    14521 tgaggaatcc ccacagcaca taagccgaca gcacggcaga caccgccaca ccgagtatcg
    14581 taacagtatg cgcaccggca cgaccgattt tgttgccgaa caaacccgca atcaacgagc
    14641 ctgccaacgg aacaagggca attatcaaat ataaagtcat gtcgttcatt tgattgaatc
    14701 cgattgattt aaaaatctat gtttgtttcg tacaaaatta cttcggaaaa acaaatccaa
    14761 cacgctccaa tcgtttgcgt gccacagcta attgctcttc agtaaataaa tcacaccacg
    14821 gcttttgtaa caccagatat tccatactgt atttcaaggc gtggactccg ccaccactca
    14881 gaatcagctc tgtaaaaccg gtctgagtct tcttttcccc cgtacttaat aatttatccg
    14941 ccgcctcttt accaccaaat tcatttacaa tttgtaaaaa tcgtgtcgcc ttgtaaggtt
    15001 gcggcaaatt caaagcctcc tgataaatat ttaacatggc tttatgaaat tcttgttcta
    15061 actgattttt atccatcatt cttcttccaa tatttcagac cggattattc ttacccagaa
    15121 tttcttttct catccgctcc cgtctgatca cctaccgaat caggtcgtct gaaacagtct
    15181 gaaatcgctt ttcagacgac cctcagcctt tttcataccc ttcgtaataa tacgactgct
    15241 cgataccttt aaagatgatt tcacggttgt ccacatcgtc ggtcaggttg tcctttaaca
    15301 gaaagcgcag ttctaaatcg ttgacggggc tgcgttccat cgcctgcaaa tacagggttt
    15361 tgcttacatt ttgccagttt acgatttttt tcaggttttt cttcagcacc aaatccagcc
    15421 agatgcgggt acttctgcca ttaccctcca aaaacggatg ggcaatgttc atttcaacat
    15481 atttggcgat gatttcttca aaagtccgct cgggcatctg ctcgatttta accaaagcct
    15541 cttttaaata catggcgttg gcaaaacgaa aaccgccttt ggaaatgttg tcttccctga
    15601 tttgacccgc aaaatcatat aagccgccga acaggtaacg gtgaatctgt tgcaggcccg
    15661 cggtggtacc gacttcgata cggtcgatgt cgccgctttc aaacaggcgg cgggcattat
    15721 gcaggctttg ttcgtctatg gatttcatcg tttttcctat cgggttttca gacggcatcg
    15781 gtgtcttgtc aggtctcgac ccaacctacc tttacccttt caactcgtcc aaatcggcaa
    15841 cattaatcgt ttgtcggttg cggtacacca ataccataat cgccaaaccg atggcagatt
    15901 cggcagcggc aacggtcaat acgaagaata cgaaaatttg tccggcagta tcgcccaaat
    15961 gttgcgagaa ggcgataaag ttgaagttca ccgccaagag catcagctcg atggacatca
    16021 gcaataccag cacgtttttg cggttcataa agatacccat tgcgctgata ccgaacagga
    16081 gcgcacccaa taccagataa tgcgtcaagg taatcatgct ttgccctccc cttccggctt
    16141 gaggccgtct gaaacttcgc tctcttcggc agattcgact tgcggtttga ccgcttccat
    16201 tttcaccaga cgcatacggc cttgatcggc gcgtacttta acttggtcgg caggatccat
    16261 gcgtttcgga ttggtggttt tacggtaaac cagcgcaatc gccgccacca tacccaacag
    16321 cagcaatacc gccgccaatt caaacggcaa caggtagtcg gtataaatac ggctgcccaa
    16381 atcgcggata ttgttgtaat cggccggaat gtctttcatc agaccaaatg cggccaggtc
    16441 ggttttcggg ttgaccagaa tcaggatcag ggcaaccgcc aacaatgtgc cgaccacacc
    16501 ggcaaccggc gcatgacgcc agaaaccggc gcgcatttct tcaatgtcga tgttcaacat
    16561 catcacaacg aacaggaaca acaccatcac ggcgccgacg taaaccacca ccagcgtcac
    16621 gcccaaaaac tcggcctgca tcagcatcca aagcatcgcg ctcacgcaga aggtcagcac
    16681 cagatgcaaa gcggcgtgga cagggttttt ggcggtaacg gtacgaaccg caccgtacaa
    16741 aacgatggcg gcaagaatat agaacagaat cgcggaaaaa gtcattcgtc tgctccttaa
    16801 cgatacggcg cgtcagcggc tttgcgtttg gcgatttcag cttcgtattt gtcgccaatg
    16861 gccaaaagaa tcggcttggt catgtgcaag tcgccttttt tctcgccgtg gtattcaaaa
    16921 atatgggttt ccacaatcgc atcggtcggg caggcttctt cgcagaaacc gcagaagatg
    16981 cacttggtca ggtcgatgtc gtaacgcttg gtgcggcgcg tgccgtcttc gcgttcttcc
    17041 gattcgatgt tgatcgccat tgcggggcaa acggcttcac acaatttaca cgcgatgcag
    17101 cgttcctcgc cgttcggata acggcgttgc gcgtgcagac cgcggaaacg cacggattgc
    17161 ggcgttttct cttcgggaaa ataaattgtg tctttgcggg caaaaaagtt tttgagcgtt
    17221 acgcccatgc cttttaccaa ctcgccgagc agaaaggttt ttactaagtt agccatatta
    17281 tgttccctca aaacagggat ttcgttaggt attcaaaatc gctttgttca gacggcctca
    17341 agatgccgtc tgaaacttat ttccacaaat tcagcggtga aatcatccac acgcccaaaa
    17401 tcacgatgta ggcgaagccg atcggaatca gcactttcca gcccaaacgc atgatttggt
    17461 cgtaacggta gcgcgggaag gttgcacgga tccacagata ccagtacaac actgccgcca
    17521 ttttcacaaa catccagaat gcggaaggcg taccgataaa gccccagctt tgcgggaacg
    17581 gagacaacca gccgccgaga aacatcagcg atgtcagcgc ggcaatcaga atcatgaaaa
    17641 tgtattcggc aaggaagaac agcgcgaatg cgaagccgga atattcaacg tggtgtccgg
    17701 caacgatttc ggactcgccc tctgccacgt caaacggtgc gcggttggtt tcggcaacgg
    17761 cggaaatcag ataaacgatg aagatgggga agagcggcag ccagttccac gagaacacgg
    17821 aaccgcccgc gatgcctttg gcttgtgcgg caacgatgtc ggagaagttc atgctgcccg
    17881 ataccatcac aacgcacacc agcgcggcac tcatggcaat ctcgtaggaa atgctttgtg
    17941 cggaagcacg cattgcaccc aagaacgaat atttggagtt ggaagcccag cccgcgatga
    18001 tcacgccgta aaccgacagc gaggtaatca tcaggatgta caaaaggccg atattgatgt
    18061 tggtcagcac ccattcttcg ttgaacggaa tcactgccca tgctgcgaaa gacggagcaa
    18121 gcgacataat cgggccgatg tagaacaggg ctttgtttga cagcttcgga cgggttacct
    18181 ctttaaacaa gagtttgaac acgtcggcaa acggctgaat cagaccccac gggccggtta
    18241 cgttcggacc gacgcgaagc tgcatgaagc cgatgacttt acgttcgaaa tacgtcaggt
    18301 aggcaacggt cagaatcagc ggaatcagga taatcacgat tttgacgatg acggatacca
    18361 ccaagcctac ggtgatgccc aaatcgccca gaccgagcgt tgcggcaaag aggttttgga
    18421 accattcttg catgatcaag ctcccgccag ttcaatagtg tccatcaacg cacccagcgc
    18481 ggcattttcg gtatgcagcg gcagatgcac cacgttttca ggcaaaccgg catcggcttt
    18541 gacagcaacc gatacgcttg cgccgttttg tttggcgaca gcggtttgtc cgtcttgcag
    18601 gcccaagcgt cccaaagtgt tcggattcac acgcgcggca ggcacggcgg catggacggt
    18661 tgcctgcaac ggtgcggaac ggcgcacgat agaatcagtg tgataaatac cgacgccgcc
    18721 gacacggacg aggcggtctg atgccgtctg aacgccctcc cctgtccatg tgctgcggtt
    18781 gtccagtttg gacggcaggc tttccgcatc caacgcatct ttcagaatcg cagcggtatc
    18841 gtggtattca aaaccttgca ggtcaaacag gttgcccaat acacgcaaca ctttccacat
    18901 cggacgcgaa tcgccgaagc cttgtaccac gccgtgaaag gattgcagac ggccttccat
    18961 attgatgaag ctgcccgatg tttcggtaaa cggcgcaatc ggcagcaata cgtcgcacac
    19021 gtccagcagc gtttcgctga caaacggtgt aaacgccatc acgcttttcg cctgtttcaa
    19081 cgcggccacg gcttttgcac cgtcaaccat atcgatttcc ggctcgacgt tgagcagcaa
    19141 gactgcctgt ttcggcgcgt ttgccatttc ggcaacgctc tcgccggagt ttacgcccaa
    19201 gacatccgca ccgacgctgt tggcgacttg cggcaaaata cccagcactg cgccggtcgc
    19261 gtctgccagc tcttgcgcgg cggcataaat ggcggcgtaa tcaggatggt tttgcacttc
    19321 cgcgcccaaa atcaccgctg ctttttcagc gtttttcagg ctggcggtaa cggcgtgttc
    19381 cgcattggca gacaggtttt tcagacggcc tgcccactcg tcgggatgtg cggcttcttg
    19441 aaacagaagc ggcataaaca attcttcctt gctgccggcc aatacgctca aggccatacg
    19501 gtctttggcg gcgcggcgca ggcgggcggt caggagcggc tgttctttgc gcaagttcgc
    19561 gccgactacc aatacggcat cgttgtcagc caaagattca atgctttgtc ccaaccattg
    19621 cgcaccctta atgccgtctg aaagacgttt gtcttgttga cgcaaacggg ttgcaaagtt
    19681 tttaacaccc aagccgtcgg caagtttttt ggccagatac agctcttcaa ccgtattcat
    19741 cgggttcgcc caaatgccga cttggttttg gttgccgtct ttggcgatac attcaatcgc
    19801 gctgcggaca tattccaatg cggttttcca atccacgtcc atccactcgc cgccctgttt
    19861 gattttcggg tttttcagac ggctttcgtg atacaggcct tcgtaggcaa aacggtcgcg
    19921 gtcggacaac cagcattcgt taatcgcttc gttttccagc ggcagtacgc ggcggacggt
    19981 atggtctttg gtttgtacaa tcaggttact gcccaaagcg tcatgggcgg aaacggattt
    20041 gcggcggttc aattcccaag tacgcgcgtt gaagcggaac ggtttgctgg tcaatgcgcc
    20101 gacaggacac aaatcgatga cgttgcccga cagctcggtt tccaccgttt tgccgataaa
    20161 gggcatgatt tcggagtgtt cgccgcgatt caccatcgca atttcttgca aaccggcgat
    20221 ttcttcagtg aaacgaacgc agcgggtgca gtggatacag cggctcattt cctcggcgga
    20281 aaccaaaggc cccatgtctt tgccgacgac ggaacgtttt tcttcggtgt agcggctggt
    20341 ggtttttccg tagcccaccg ccaaatcctg caactggcat tccccgcctt ggtcgcaggt
    20401 cggacaatca agcggatggt tgatgagcag gaactccatc acgccttcct gcgcctctcg
    20461 ggcttttgcc gaatgcgtac gcacaatcat gccgtctgta accggcgtgg cacaggcagg
    20521 caggggtttt ggggcttttt ctacgttcac cagacacata cggcagttgg cggcgatgga
    20581 aagttttttg tggtaacaga aatgcggaat ataagtaccg agcttgtgcg cggcttcaat
    20641 caccgtcgcg ccctgctcca cagatacttg tttgccgtcg atttcgattt gtaacatggt
    20701 tcgttcctag ttacgggtat ttgataaata attttttatg gagatcgtct gaaaaatggg
    20761 tttcagacgg ccttttgagt ttcattgaca atcaatactg tttaaaacgt ttactgccac
    20821 cttgccgtca ttcccgcgta ggcgggaatc cattcttggg ttttcggtaa ctgcttttca
    20881 aatatcgggt tctgtaaatt ccactatgga ttcccgccta cgcgggaatg acggcaaagt
    20941 taaattttta gcattttgct tttaaccaat ataaaaccaa ctaaaactat aacttattta
    21001 ttatattttt aaatgactta aataactctt taacagcacg ctcattgtct tcatctgtag
    21061 gctcaaactt ttcacaccaa ttaattatat ttacatcgcc atgcaataac tctgcataac
    21121 atcctactgc atcgcaacta ccaccccgtg tccttgacgt gccaaaaata aaatcacaat
    21181 tatgcgaata aagtttgtat aaatttctcc taacctcgta ttcattatca ccagatgaat
    21241 aaaaaccaat cctctgacct tcatgatcaa atactgcaac aaaatctaag ccgtttccat
    21301 gtctttcaaa aaagactaga aatttagaaa atttccgaca aatctgatta aaaagcgtat
    21361 tgagtgtcgt actcttacct ttgttggctg caccgtatag aataaaaatt ttagcttcca
    21421 ccattaccac cctccccact tatgctcttt catcggtccg ccgtgttcga tgtaatgcac
    21481 aaactcatca cggaaatgct tggtaaagct tcggacgggg aagacggcgg catcggcaag
    21541 ggcgcagatg gtgcggcctg ccatttggtt gccgacagaa tccagcaaat ccaaatcttc
    21601 cattttacct ttgccttcta cgatgcggtg gacgatgcgg taaagccagc cggtgccttc
    21661 tcggcaaggc gtacattggc cgcaagactc gtcgtagtag aagtagctca ggcgctcaag
    21721 ggctttcacc atgcacacgt cttcgtccat gacgataatc gcgccagaac ccagcatcga
    21781 gcctgctttg gaaatcgagt cgtagtccat atttgtctgc atcatgatgt cggcaggcaa
    21841 tacgggcgcg gacgaaccgc cgggaatgac ggctttgagt tttttgccgc cgcgcatacc
    21901 gcctgccatt ttcaagactt cggcaaacgg tgtgcccaat ggcacttcat agttgcccgg
    21961 acgctcgaca tggccggaaa tacagaataa tttggtaccg cctgcattcg gaataccttt
    22021 atcggcaaat gcctgtccgc cgtcacggat aatgaatgaa acggaggaga aagtttcggt
    22081 attgttgatg gtagtcggtt tgccgtacag gccgaacgaa gcagggaatg gcggtttaaa
    22141 gcgcggctgg ccttttttgc cttccagcga ttcaagcaat gcggtttctt cgccgcagat
    22201 gtatgcaccg tagccgtggt gggcgaagag ttcgaattca aaatccgaac ccaaaatatt
    22261 tttacccaaa aagcctgcgg cacgcgcctg ctccaaagcg gcctcaaagc gttggtagcc
    22321 ttcaaaaatt tcgccgtgga tatagttgta accggctttc gcgcccatcg cgtaaccggc
    22381 gataatcatg ccttcaatca gggcatgggg gttgaacata ataatgtcgc ggtctttaaa
    22441 tgtacccggc tcgccttcgt cggtgttgca aacgacgtac ttttcgccgg ggaaagaacg
    22501 gggcataaag ctccatttca aaccggtcgg gaagcccgca ccgccacgac cgcgcaagcc
    22561 ggaggttttg acttcgtcaa tcacatcggt ttgcgagatg ttttcggaca ggattttacg
    22621 cagggcggta taaccgccac gtttgacgta ttcgtccaat gtccagcaat cgggattggc
    22681 ggtatccact tggtcaaaaa tcacgcctga ttggtaaata gccatttttg gtgtgcctgt
    22741 ttgttttcgt atcggttgcg gccgctgttt cagacgacct taagatgtct ttgtgtaccg
    22801 gcttgtaacg tcgtctgaaa taaaatctag tttatcaaat cgatattttc agacgacctt
    22861 taatcgtttt tgttggatct cgaccacttg cttgtctgcc gtcattcccg cgcaggcggg
    22921 aatccatcct caatggtaag caatgtctta ttaaattcag aaaccgaatc ttaccggtgg
    22981 attcccgcct gcgcgggaat gacggcattt cggtatttca gtagggcgga ttcttaaatc
    23041 cgacattttg cctttttacc cactctgtcg gatacaagta tccgacctac gtttaaatcg
    23101 tcgtttcaga cgacctactc caactccgcc agtttcttct caatcgcctc ttcagtcata
    23161 aagctgcaca ttttatgatt attcaacaac ataacaggag catcgccgca cgcccccatg
    23221 cattcgcctt cgacaagggt aaatttgccg tcaggcgtgg tttcgccgta gctgataccg
    23281 agtttttgtt tgaggtattc gccggttgcc ataccgccgc gcagggcgca gggcaggttg
    23341 gtacaaacgg tcagtttgta tttgccgaca ggctcaaggt cgtacatatt gtagaaggta
    23401 gcgacttcgt aggcttgtgc aggcgagatg ccgatatagt ctgcaacaaa ggcgatggtt
    23461 tcgggggcga gccagccttt ttcggtttgt gcaatacgca atgcgcccat aatggcagag
    23521 cggcgttggt cggcaggata ttttgccaac tcaatatcaa tttgttttaa ggattctgcg
    23581 gataacatta tcggtcaacc tccccgaata cgatgtcctg cgtaccgatg atggcaacga
    23641 cgtcggcgag catgtggcct tttgccattt cgtccatgcc ttgcagatgg gcgaagccgg
    23701 gtgcgcggat tttcaggcgg tagggtttgt ttgcgccgtc tgaaatgatg taaacgccga
    23761 actcgccttt cggatgttcg acagcggtgt aggtctcgcc ctcgggaacg tgcataccct
    23821 cggtaaagag tttgaaatgg tgaatcaggt cttccatacc tgttttcatt tcggtacgtt
    23881 tgggcggagc aaatttgtgg tttgtggtaa tgaccggacc cggattgaca cgcaaccact
    23941 cggaacattg tttgatgatg cgtacggatt gacgcatttc ttccatacgg cagaggtagc
    24001 ggtcgtagca gtcgccgttc acgccgacag ggatgtcgaa atccattttg tcgtacactt
    24061 cgtaaggctg tgtcttacgc acgtcccatt ccacgcccga accgcgcaac atcacgccgg
    24121 taaagccttt ttgcatggca cgttcggggg agacgacgcc gatgccgacg gtacgctgtt
    24181 tccaaatacg gttgtcggtc aggagggttt cgagtgtgtc gatgtttttc gggaaacgtt
    24241 cgcagaaggc atcgataaag tcgagcatcg tgccttcgcg ggattcgttg agctgcttca
    24301 atactttggc attgcggaat ttgctgccct cgtatttggg catgaagtcg ggcaggtcgc
    24361 ggtaaacgcc gccgggacgg aagtaggctg cgtgcatacg tgcgccggac acggcttcgt
    24421 acaagtccat caactcttcg cggtcgcgga aggcataaag gatggcggtc atcgcaccaa
    24481 tgtcgaatgc gtgcgaaccg atgcccatca aatgattgag gatgcgcgtt acttcggcga
    24541 acatcacgcg gatatattgg gcgcggatag gtacatcgat accggcaagt ttttctactg
    24601 ccaagcaata cgcctgctcg ttgaccatca tggaaacata gtccaagcga tccatatagg
    24661 gcagggcttg cagataggtt ttggtttcag ccagtttttc agtacctcgg tgcaggaggc
    24721 cgatatgcgg gtcggcacgg acgatttgtt cgccgtccag ctccaaaatc atacgcaata
    24781 cgccgtgcgc cgcagggtgt tgcgggccga agttgatggt gtagtttctt aatttattgg
    24841 ccaccgtagt tctcctcacg gacgatacgc ggcgtgatct cgcgcggctc gatggtaaca
    24901 ggttggtaaa tcacgcgttt ttgctcttcg tcgtaacgca tttccacata gccggaaatc
    24961 gggaagtctt tgcggaacgg atgtccgacg aaaccgtaat cggtcaggat gcggcgtagg
    25021 tccgggtggt tgttgaacat gatgccgtac atatcgaagg cttcgcgttc gtaccaatcc
    25081 gcgctgttgt aaatatcgac tacggattcg actacaggga agtcgtcgtc tgaaacccag
    25141 acgcgtacgc ggatgcgttg attgttttta acggaaagca actgactgac gacggcaaag
    25201 cgtttgccct gccatacttc gtttttgtaa gtgctgtaat cgacaccgca caaatcgacc
    25261 agaagctcga aatgcagctc ttcatgatcg cgcaatgcgg tcatgactga aatatagtgt
    25321 tcgggcagac actcgacggt aatctcgccc aaagctgaaa tgactttgcc tgcctgattg
    25381 cccaaaacgc ggctgacggt ttcgtataag ttttgaatgc ttgccatatc ttcctcctct
    25441 tactcgtcac gcgcgatggt ggaagtgcgc ttgatttttt gttggagctg aatcaggccg
    25501 taaatcaggg cttccgcagt cggcggacaa cccggcacat aaacgtctac cggcacgacg
    25561 cggtcggcac cgcgcacaac ggaataagaa tagtgataat agccgccgcc gttggcacac
    25621 gagcccatag acaataccca gcgtggctcg gcgagctggt cgtatacacg gcgcagggct
    25681 ggcgccattt tgttggtcag cgtacctgcc acaatcatca ggtcggcctg acgtggggac
    25741 ggacggaaaa tgataccgaa acggtcaagg tcataacgcg ccatacccgc atgcatcatt
    25801 tccacggcgc agcaggccaa gccgaaagta accggccaca acgaaccggt acgcatatag
    25861 ttcaacaccg tatccgcgct ggtggtgatg aaaccttttt tcaaaacgcc ttctattccc
    25921 attccagcgc accttttttc cattcgtaaa caaagcctac cgtcagaacg acgataaaca
    25981 ccaacataga ccagaagccg tacgcgccca aatctttgaa cacaactgcc cacggcaaca
    26041 tgaacgcgac ctccaaatca aacaggatga agaggatggc gacgaggtaa tagcgcacgt
    26101 caaacttcat cctcgcattt tcaaaggctt cgaaaccgca ttcgtaaggc gcgtcttttt
    26161 cggcatagtg gcgtttcggg cctaaaatcg tgccgagcag gataaacagt acgccggccg
    26221 cgaggccgac gaggataaag acaaagacgg gaaagtaagc ggacaacatg gttacaccca
    26281 aatccgttaa caaaatttct acaataattt cgtattttag cgaatttcaa aaaccattaa
    26341 aaggtaaata tcggcaaaac gcccaaaaaa acccaataaa tacaatcatg ttatgataac
    26401 gattcttatt tgattttata aggtcatata aatttttaca ccgtaattcg tg
//
LOCUS       DAXHMX010000009        26417 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHMX010000009 DAXHMX010000000
VERSION     DAXHMX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.009"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(131..466)
                     /locus_tag="ACFM4S_000291"
     CDS             complement(131..466)
                     /locus_tag="ACFM4S_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6050049.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            595..1500
                     /locus_tag="ACFM4S_000292"
     CDS             595..1500
                     /locus_tag="ACFM4S_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFC6050050.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGNGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1562..2050)
                     /locus_tag="ACFM4S_000293"
     CDS             complement(1562..2050)
                     /locus_tag="ACFM4S_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6050051.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2197..3099
                     /locus_tag="ACFM4S_000294"
     CDS             2197..3099
                     /locus_tag="ACFM4S_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6050052.1"
                     /translation="MDTAKKDILGSGWMLVAAVCFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3140..4270)
                     /locus_tag="ACFM4S_000295"
     CDS             complement(3140..4270)
                     /locus_tag="ACFM4S_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6050053.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4475..7444
                     /gene="alaS"
                     /locus_tag="ACFM4S_000296"
                     /pseudo
     CDS             4475..7444
                     /gene="alaS"
                     /locus_tag="ACFM4S_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
     gene            complement(7532..8554)
                     /locus_tag="ACFM4S_000297"
     CDS             complement(7532..8554)
                     /locus_tag="ACFM4S_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6050054.1"
                     /translation="MLTKKEFEKFLDEVCTVLRQEAKNAPFKSQDIFENRVRALCIAK
                     IPAYADIVINPEPVPQIFPDVPIGEYGVEVKFTLKDTWRSVANSIQEKNKADGVQHIY
                     VIFGKMGGIPDVKWQLYEDSVIHVRTSHVPRFEVEIESDRPSLFEGFGISYAEFADLD
                     MHEKMEYIRKYARNRLKEGERLWWIEDIDAVDSHDLPLEVRLYTSLPQEEKIRLRAEA
                     ALVSPRIVCSGRAKHKYDNAVLYLITYRGVLCHQARDLFSAGSVANSAEDYRGGNYVE
                     KSLKLIEHEIEKAALEMDDALIVEYWGESVPTEKRLEYWIKMLDDLADGWIPSESLFN
                     GRYKAK"
     gene            complement(8608..9759)
                     /locus_tag="ACFM4S_000298"
     CDS             complement(8608..9759)
                     /locus_tag="ACFM4S_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6050055.1"
                     /translation="MTEFARKNWNKLAGDAIKAKMHEHGIGFIQLSELFLEQGESETA
                     NAICNKVNRGTFSHAFYLKCMELMTKQEKQEDREKKYKIISLFSGCGGMDLGFLGGFE
                     FLNRTYDKHDFDIIWANEIDEHAVRTYRHNLGNHIVHGDIWQHLDEIPTSADIIIGGF
                     PCQDISINGKGAGISGSRSGLYKAMLSAVERVRPKIFVAENVKGLLMKHHRKDLETVL
                     SDFRNLGYQVDYYLLNAADYGVPQTRERVIIVGRLPEIPEIQIEKTNTSNSYVTARQA
                     IGDLVDKVEDEIANHIWSRAEKSPDQGSRKLDAERAGYTIRAECHGNIQWHYELPRRI
                     SMREAARIQSFPDNFIFQAKLRATERQIGNAVPPVLGWHIAAAVSKALK"
     gene            10382..10612
                     /locus_tag="ACFM4S_000299"
     CDS             10382..10612
                     /locus_tag="ACFM4S_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050056.1"
                     /translation="MFKTLKSIWQGLTQKGKIFPYQLAFTLLIPLRNWALSPQQITQR
                     LHFVPHHRICCGAGYFSCVGCASAHRFLIFAC"
     gene            10853..11797
                     /gene="tehA"
                     /locus_tag="ACFM4S_000300"
     CDS             10853..11797
                     /gene="tehA"
                     /locus_tag="ACFM4S_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFC6050057.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAVASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GILSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRG"
     gene            complement(11871..12554)
                     /locus_tag="ACFM4S_000301"
     CDS             complement(11871..12554)
                     /locus_tag="ACFM4S_000301"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6050058.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVIPDGENLKVTLDRVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            12852..15155
                     /gene="parC"
                     /locus_tag="ACFM4S_000302"
     CDS             12852..15155
                     /gene="parC"
                     /locus_tag="ACFM4S_000302"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6050059.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNSAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            15176..16767
                     /locus_tag="ACFM4S_000303"
                     /pseudo
     CDS             15176..16767
                     /locus_tag="ACFM4S_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            16760..17227
                     /locus_tag="ACFM4S_000304"
     CDS             16760..17227
                     /locus_tag="ACFM4S_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6050060.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQADKRLGISFRSMRYRMERLNIG"
     gene            17331..18083
                     /locus_tag="ACFM4S_000305"
     CDS             17331..18083
                     /locus_tag="ACFM4S_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6050061.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLTEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAKPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEAVLLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYEADKG"
     gene            complement(18599..18805)
                     /locus_tag="ACFM4S_000306"
     CDS             complement(18599..18805)
                     /locus_tag="ACFM4S_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6050062.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(18891..20060)
                     /gene="metZ"
                     /locus_tag="ACFM4S_000307"
     CDS             complement(18891..20060)
                     /gene="metZ"
                     /locus_tag="ACFM4S_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6050063.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAMYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            complement(20205..20966)
                     /locus_tag="ACFM4S_000308"
     CDS             complement(20205..20966)
                     /locus_tag="ACFM4S_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050064.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIESGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            21066..21317
                     /locus_tag="ACFM4S_000309"
     CDS             21066..21317
                     /locus_tag="ACFM4S_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050065.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            21369..22175
                     /locus_tag="ACFM4S_000310"
     CDS             21369..22175
                     /locus_tag="ACFM4S_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6050066.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDEQAAK"
     gene            22482..24005
                     /locus_tag="ACFM4S_000311"
     CDS             22482..24005
                     /locus_tag="ACFM4S_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC6050067.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGAAACEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            24103..25515
                     /gene="trkA"
                     /locus_tag="ACFM4S_000312"
     CDS             24103..25515
                     /gene="trkA"
                     /locus_tag="ACFM4S_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6050068.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAETGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            26284..26359
                     /locus_tag="ACFM4S_000313"
     tRNA            26284..26359
                     /locus_tag="ACFM4S_000313"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26318..26320,aa:Glu,seq:ttc)
BASE COUNT         6437 a         7388 c         6480 g         6112 t
ORIGIN      
        1 cgcttacccc ttgtttctca aatgccgtct gaaaaaacgg ctgcatatgt tgtctacaaa
       61 atatcgggaa gcataacaga caaaatgccg tctgaaatat tttcagacgg catttcttat
      121 ccgaaacgga ttatttttgc gtttcaaccg cttccaatgc acgcagggcg taagtgtaag
      181 cggcaccggc attcagggcg atggcggttg ccaatgcgcc tgcgatttcg ctgtcggtcg
      241 caccggcttt ggcggcagcg gcggcgtgaa cgctgatgca gctctcacaa cgtgtggtaa
      301 tggcaacggc gatggcaatc agttcgcgcg ttttggcatc aagtgcttcg gcggcggcgg
      361 cctgttccaa tgcgccgtag gcctgcagca ttttaggatg cgccttaccc agctcgccga
      421 acgatttttt aaccaatgcg gtatgttctt tccaatcttt aaacattttc ttttcctttc
      481 tcttgcgttt aaccctgata cgcgcttgcg tatctgtttt cgatgtgcgt attattgcaa
      541 ttattcagtt gtgtttctcg tttaatcatc tcattttatg gttcaaaaag atttatggac
      601 attctggaca aactggtcga tttcgcccaa ttgacgggca gtgtggatgt gcagtgcctt
      661 ttgggcggac aatggtcggt acggcatgaa accttgcaac gcgaaggact ggtacacatt
      721 gttacatcgg gcaacggcta tctctgcatc gacggcgaaa cttccccgcg tccggtcagt
      781 acaggggata ttgtattttt cccgcgcggc ttgggtcatg tgttgagcca cgacggaaaa
      841 tgcggagaaa gtttacaacc ggatatgcgg cagcacggtg cgtttacggt caaacagtgc
      901 ggaaacggac aggatatgag cctgttttgc gcccgtttcc gctacgacac ccacgccgat
      961 ttgatgaacg ggctgcctga aaccgttttt ctgaacattg cccatccgag tttacagtat
     1021 gtggtttcaa tgctgcaact ggaaagcaaa aaacctttga cggggacggt ttccatggtc
     1081 aacgcattgt cgtccgtcct gctggtgctt atcctgcgcg cctatctcga acaggataag
     1141 gatgtcgaac tctcgggcgt attgaaaggt tggcaggaca aacgtttggg acatttaatc
     1201 caaaaggtga tagacaaacc ggaagacgaa tggaatgtcg acaaaatggt ggcggctgcc
     1261 aatatgtcgc gcgcgcaact gatgcgccgt ttcaaaagcc gggtcggact cagcccgcac
     1321 gcctttgtga accatatccg cctgcaaaaa ggcgcgttgc tgctgaaaaa aaacccggat
     1381 tcggttttgt cggtcgcact gtcggtaggc tttcagtcgg aaacgcactt cggcaaggcg
     1441 ttcaaacggc aatatcacgt ttcgccgggt caataccgga aagaaggcgg gcaaaaataa
     1501 atcggggctt caaacgcaaa tgccgtctga aaaggctttc atacagcatt tgcgtaccgc
     1561 gtcatttcaa gggctgcatc ttcatcactt ccatcaaaaa gttggtaaat gcggggttgt
     1621 tgggtttgac atccatattt ttccaacgct gctgccagcc gcgcaaggca ttctggatat
     1681 acagcttgga ctgttccgta ttgattgcgc cccgctggct gtctatcgcc gaacgcaggt
     1741 agatttcata catactgtca tcgacggcat tgcgtccgac caggcgtttt ctgaagttgt
     1801 tcagatattg cgccgcctga accttggtca ttttaccgat acccacctga tagcccaagc
     1861 gcgtcgcttc atcgctgatt ttggcaacat ccgtccaatg cgaagaggca aggcggaaac
     1921 cttttgcagg tgcttccgtt ttgacggtat tgataggatt cacggggatt tccgtcaatg
     1981 tgggcacata aatagactgg cagccggaaa gaactgccgc aatggaaaga gggataaggt
     2041 attttttcat gcccccatta taatcaagtt tgccttgaga aaacaaattg ttcggcaaga
     2101 aaaataaaat ttcggcatca gaagcaggca aaaacacatt ccacaagcct tgccgcaagg
     2161 tttacaatcc gaccgtcctt atcgcaacga ccgtttatgg ataccgcaaa aaaagacatt
     2221 ttaggatcgg gctggatgct ggtggcggcg gtctgcttca ccattatgaa cgtattgatt
     2281 aaagaggcat cggcaaaatt tgccctcggc agcggcgaat tggtcttttg gcgcatgctg
     2341 ttttcaaccg ttgcgctcgg ggctgccgcc gtattgcgtc gggacacctt ccgcacgccc
     2401 cattggaaaa accacttaaa ccgcagtatg gtcgggacgg gggcgatgct gctgctgttt
     2461 tacgcggtaa cgcatctgcc tttggccacc ggcgttaccc tgagttacac ctcgtcgatt
     2521 tttttggcgg tattttcctt cctgattttg aaagaacgga tttccgttta cacgcaggcg
     2581 gtgctgctcc ttggttttgc cggcgtggta ttgctgctta atccctcgtt ccgcagcggt
     2641 caggaaacgg cggcactcgc cgggctggcg ggcggcgcga tgtccggctg ggcgtatttg
     2701 aaagtgcgcg aactgtcttt ggcgggcgaa cccggctggc gcgtcgtgtt ttacctttcc
     2761 gtgacaggtg tggcgatgtc gtcggtttgg gcgacgctga ccggctggca caccctgtcc
     2821 tttccatcgg cagtttatct gtcgtgcatc ggcgtgtccg cgctgattgc ccaactgtcg
     2881 atgacgcgcg cctacaaagt cggcgacaaa ttcacggttg cctcgctttc ctatatgacc
     2941 gtcgtttttt ccgccctgtc tgccgcattt tttctgggcg aagagctttt ctggcaggaa
     3001 atactcggta tgtgcatcat catcctcagc ggtattttga gcagcatccg ccccactgcc
     3061 ttcaaacagc ggctgcaatc cctgttccgc caaagataaa aaatgccgtc cgaacatcct
     3121 tcagacggca tatcgggctt tatttccccg ccttcacatc ctgccactgg cgcaccataa
     3181 acttcaatgc cgccggctgg ataggcacca tgataaagct gtttttcaaa tcctcctcgg
     3241 ttgggaaaat cgtattgtcg tttttaaatt cgtcttccat cagctcacgc gccggcttgc
     3301 tcgaaggcgc ataagtaacg aaattgccgt ttttcgccga cacttccgga tcgaggaagt
     3361 cgttgatgta tttgtgcgcg ttggcgacgt ttttcgcatc tttcggaatc acgaaagaat
     3421 ccacccaaat ccccacgccc tctttgggca tcatcacgcg gattttttcc ttgccgcccg
     3481 cttcttcggc acggcgtttg gcgatgttca aatcgccgcc gaaaccgatt gttacgcagg
     3541 tatcgccgcg cgccaaatca tcgataaagc cggacgaagt aaagcgtttg atattggggc
     3601 ggtttttctt gagtagggcg gttgcctccc tgatgtcttc cgtattgctg ctgttcgggt
     3661 ttttacccaa atagttcaac accataggat agatttccgc cgcgctgtcc aaatagctga
     3721 tgccgcattg cttgagtttg gacgtgtatt cggggtcgaa caccaaatcc cactggttgt
     3781 ccggcagctt gtccgtaccc aaagcctttt tcacgcgttc ggtattgatg gcgaaggtat
     3841 ttgtccccca ataaaacggc acggcgtatt cgtggccggg atcgaccccg tccatcagcc
     3901 tcatcatttc agggttgagg tgtttataat tgggaatcag cgacttatcg attttctgat
     3961 acgcacctgc cttaatctgc ctgcccacaa acgcattgga cggcgcgaca atgtcgtaac
     4021 cggacttgcc tgtcagcacc ttgctttcca gcgtttcatc gctgtcgtac acatcataag
     4081 taaccttgat gccgtttttc ttttcaaaat cggcaacggt ttccggatcg acatattccg
     4141 accagttgta aattttcaat acgttttggt tttccgccgg tgccggtttt tcggcaggcg
     4201 gtttgtccga accgccgcac gctgcaagca gcaaagcagt caggacggcc aggggcagat
     4261 gtttggtcat tatcattcct tgcatatcgg gttggagaaa gcggccatta tagccgatat
     4321 tggcaacagg gcttcagacg gcattcaaaa tcccgccaca ctcttccgaa aaccgccgct
     4381 tccatagcta gaaacaggga tttgcggtaa gataccgccg ttcgttttcc ctgcttttac
     4441 catgacaaga catttgagag acattgaaaa aattatgaaa acctccgaac tgcgccaaaa
     4501 attcctaaaa ttttttgaaa ccaaaggcca caccgtcgtc cgctcttcca gcctcgtgcc
     4561 gcacgacgac ccgaccctgc tgtttaccaa cgcgggcatg aaccagttta aagacgtatt
     4621 cttaggtttc gacaaacgcc cgtacagccg cgccaccacc gcgcaaaaat gcgtacgcgc
     4681 aggcggcaag cacaacgact tggaaaacgt cggctacacc gcccgccacc acaccttctt
     4741 tgaaatgatg ggcaacttct ccttcggcga ctacttcaaa cgcgacgcca tccatttcgc
     4801 ttgggagttt ctgacttccc ccgaatggct caacatccct aaagacaaac tgttggcgac
     4861 cgtttacgca gaagacgacg aagcctacaa catctggttg aacgaaatcg gtatgccgtc
     4921 tgaacgcatc gtccgcatcg gcgacaacaa aggcgcgaaa tacgcgtctg ataatttttg
     4981 gcaaatgggc gacaccggcc cttgcggccc ctgctccgaa attttctacg accacggcga
     5041 agaaatttgg ggcggcattc ccggcagccc cgaagaagac ggcgaccgct ggatcgaaat
     5101 ttggaactgc gtatttatgc agttcaaccg cgacgaacaa ggcaatatga atccgctgcc
     5161 caagccgtcc gtcgataccg gcatgggttt ggagcgcatg gcggcagtaa tgcaacacgt
     5221 ccacagcaac tacgaaatcg acttgttcca agacctgctc aaagccgttg cccgcgaaac
     5281 cggcgcagct ttcagcatgg aagaaccaag cctaaaagtc atcgccgacc acatccgctc
     5341 ctgctcattc ctgattgcag acggcgtatt gccttccaac gaaggccgcg gctacgtatt
     5401 gcgccgcatt atccgccgcg ccgtgcgcca cggttacaaa ctgggtcaga aacaagcatt
     5461 tttctacaag ctcgtgcctg atttggtgaa agcgatgggc gacgcgtatc ccgagttgaa
     5521 agagaagcag acgcaaatta tggaagccct gcgcgcggaa gaaagccgct tcggcgaaac
     5581 gctggaaaaa ggcatgggct tgttcaacca agtctataat gaattgaaat tcaatcaaat
     5641 ttttgatctt ctggcgaaag aatttaaggt tacacctgtt gaagatattg atcttgatat
     5701 gtttagtaca agaaattttg taggaagaag tgctgaaatt ccttatatat caaatcaaca
     5761 ccctaacgtg gattatcccg ctcctatggg gaacgtaaat gaaaaaattt atgctatgcc
     5821 agtgagatta acagatggaa gaattggtat taatattttt cctactgatg aaactgcacg
     5881 cctaatcctt agcaatgatt ggaatagttt gcgcttctat tctttttgta attcgttgat
     5941 tacttattat caacagaaag gtcaattaga aacattaaaa ttgaacggcg aacacatctt
     6001 caaactctac gacacctacg gtttccccta cgacctgact gccgacatct gccgcgaacg
     6061 caatatcgaa ctggacgaag caggcttcga gcgcgaaatg gaagcccaac gcacacgcgc
     6121 acgcgccgca caaagcttca aagccaacgc ccaactgcct tacgacggtc aagacaccga
     6181 gtttaaaggt tatagcgaac gccaaaccga atccaaagtc ctcgccctct acaaagacgg
     6241 cgagcaagtc aacgaattga acgaaggcga cgaaggtgcc atcgttatcg actttacccc
     6301 gttctatgca gaatccggcg gccaagtcgg cgacgtcggc tatatctttg caggcgaaaa
     6361 ccgctttgaa gtacgcgata cccaaaaaat caaagcagcc gtattcggcc aattcggcgt
     6421 acaaacttca ggccgtctga aagtcggcga cagcgttacc gccaaagtgg acgacgaaat
     6481 ccgcaatgcc aatatgcgca accacagcgc gacccacctg atgcacaaag ccctgcgcga
     6541 cgtattgggc ggacacgtcg aacaaaaagg ctctttagtt accgccgaat ccacccgttt
     6601 cgacatttcc catccccaag cggtcactgc cgaagaaatc gccgaagtcg aacgccgcgt
     6661 caacgaagcc gttttggcga acgttgccgt caatgcagcc attatgagca tggaagacgc
     6721 gcaaaaaacc ggcgcgatga tgctcttcgg cgaaaaatac ggcgaagaag tgcgcgtact
     6781 gcaaatgggc ggtttctcta ccgaattgtg cggcggcaca cacgtttcac gcaccggcga
     6841 catcggcctc ttcaaaatca tcagcgaagg cggtattgcc gcaggcgtgc gccgtatcga
     6901 agccatcacc ggcctgaacg cactcaaatg ggcgcaagag caagagcgtt tggtgaaaga
     6961 cattattgcc gaaaccaaag cccaaaccga aaaagacgta ctggcaaaaa tccaagcagg
     7021 cgcggcacac gccaaagcat tggaaaaaga attggcacgc gccaaagccg aactcgccgt
     7081 ccacgcaggc gccaaactct tggacgatgc aaaagacttg ggcgcagcca aactcgttgc
     7141 cgcccaaatc gaagccgacg cagccgccct gcgcgaaatc gttaccgatt taaccggtaa
     7201 atccgacaac gccgtgattc ttttagcggc agtaaacgac ggcaaagtct ccctgtgcgc
     7261 cggcgtatcc aaaccgttga ccggcaaagt gaaagcaggc gatctggtta aatttgcagc
     7321 cgaacaagtc ggcggcaaag gaggcggcag accagatttg gcacaggcag ggggcacgga
     7381 tgcaggcaaa ttgcccgccg tgttggatag cgtgaaagac tgggtcggcg cgaagctggc
     7441 ttgatggaga aaggcagcct gaaagggatt caggctgcct tttgtgttaa agatgccgtc
     7501 tgaaagatat tttcagacgg cctctgtcag tttatttggc tttataccta ccattgaata
     7561 gcgattcaga aggtatccag ccatctgcca aatcatccaa catcttaatc caatactcta
     7621 ggcgtttttc tgtcgggacg ctttctcccc aatattcgac aattagagca tcatccattt
     7681 ccaaagcagc tttttcaatt tcatgctcaa tcaattttaa agatttttct acatagtttc
     7741 cgccacggta gtcttccgcc gagttggcaa cgctgcccgc actgaataag tcccgcgcct
     7801 gatgacaaag cacaccgcga taggtaatca gatacaggac ggcattatcg tatttgtgct
     7861 tcgccctgcc tgagcagaca atacgggggg agactaatgc tgcttcagct cggaggcgga
     7921 ttttttcttc ctgcggcagg gaagtgtaaa ggcgcacttc caaaggcaga tcatggctgt
     7981 ctactgcgtc aatgtcttct atccaccata accgctcgcc ttctttcagc ctgttacggg
     8041 catattttcg gatatattcc attttttcat gcatatccaa atcggcaaat tcagcatagg
     8101 aaataccgaa accctcaaac agagacggtc gatcagattc tatttctacc tcaaagcgcg
     8161 gaacatgcga tgtgcggaca tgaattacgc tatcttcgta aagttgccat ttcacatcgg
     8221 gaatgccccc catttttccg aatatcacat agatatgctg tacaccgtca gctttgtttt
     8281 tctcttggat actgtttgct acgcttcgcc aagtatcttt tagcgtgaat ttgacttcta
     8341 cgccatattc ccctattgga acatcgggga aaatttgcgg cacaggttca ggattgataa
     8401 ctatgtcagc ataagcggga atttttgcaa tgcacagcgc acgaactctg ttttcaaaaa
     8461 tatcttggga tttgaaaggt gcgttttttg cctcttgcct caatacggtg caaacctcgt
     8521 ctaaaaactt ctcaaactcc ttttttgtca gcataatgca ttaccactta aaaatataaa
     8581 gtagtaattt tatcttattt aacagggtta tttaagtgcc ttactgacag cggcggcgat
     8641 gtgccaccct aaaacaggag ggacggcgtt gccaatttgc cgttctgtgg cacgcaattt
     8701 tgcctggaaa ataaaattgt cgggaaacga ttggatgcgc gccgcctcac gcatggagat
     8761 gcggcggggt aattcgtaat gccactgtat gtttccatgg cattctgccc taatagtgta
     8821 gcccgccctt tcggcatcca attttcgact accttgatcg gggctttttt ctgctctact
     8881 ccaaatatgg ttggcaattt cgtcttctac tttgtcgacc aaatcgccta tagcctgtct
     8941 ggctgttaca tagctgttgc tggtatttgt tttttcaatc tgtatttcgg gaatttcagg
     9001 tagccttcca acgataataa ctcgctcccg agtctgtgga acaccgtaat cggctgcatt
     9061 cagcaagtag taatctacct gataacccaa attgcgaaaa tctgaaagca ctgtttccaa
     9121 atctttccga tgatgcttca tcagtagccc tttgacattt tcagcaacaa atattttggg
     9181 gcgaacgcgt tcaacagcag acaacatggc tttatacagc ccgctgcggc tgcctgaaat
     9241 acctgcacct ttaccattaa tagaaatatc ctgacaagga aagccaccga taatgatgtc
     9301 tgcgctagtg ggtatttcat ccaaatgctg ccaaatatca ccatgtacaa tgtgatttcc
     9361 taagttgtgg cggtaggttc taacagcgtg ttcgtcgatt tcgttcgccc aaataatatc
     9421 aaaatcatgt ttgtcataag tacggtttaa aaactcaaag ccccccagaa aacccaaatc
     9481 cattccgcca caaccgctaa ataaagaaat aattttatat tttttttcgc ggtcttcttg
     9541 cttttcctgt ttggtcatta actccataca tttaagataa aaagcatggg aaaaagtacc
     9601 tctatttact ttgttacaaa ttgcatttgc cgtttcactt tccccttgct ccaagaataa
     9661 ttctgataat tgtataaagc caattccatg ctcatgcatt ttggctttta tggcatctcc
     9721 agctaacttg ttccaatttt ttcgtgcaaa ttcggtcatg gttaatcttt cagtcattca
     9781 taaatgacat tattttagcg ttttttataa tttaagccaa gatttaaaat ctacctttag
     9841 gtttacaggc cgtctgaaaa caaaaatctc gttttcagac ggcctttttc cgctaaaatc
     9901 cccctaccgt ccaacccgaa acaaggagaa ccatcatgca agttcctact tccgccatca
     9961 tcaacggcga atttgccgac aaatacggca aacgcggcag caagccgtag ccagcacccc
    10021 tgtcaaccgc gtgcgtggct agaccacaca ccctaccact gcgggcgacg caaaccttaa
    10081 gattgaggtg tagggtgtgt gcggtacgca cgcacgcggt ttttgttact accgaatgat
    10141 tccaaaccat gccgtaatcc agtcagcagc aaactcagct tgggtcatgt accaacaaaa
    10201 ctcaatatca aaggccgtct gaagaataat tttcagacgg cttctctcta tcaaaagcag
    10261 cctgcacatt caaaaatcga agtgcaggct gctttttgtg atgattggaa aaaacacaca
    10321 ctatttttat actgtatatt gtgcaggtac aaagtaaaac gaattaccca aagaaattcc
    10381 catgtttaag acgctcaaaa gcatttggca gggacttacg caaaaaggca aaatcttccc
    10441 gtaccaactg gcattcacgc tgctgattcc tttgcgtaac tgggcgcttt caccgcaaca
    10501 aatcacgcaa cggctgcact ttgttccgca ccaccgaatt tgctgcgggg cgggttattt
    10561 cagttgcgta ggatgtgctt cagcccaccg tttcctcatt tttgcttgtt aattggtggg
    10621 ctgaagccca cactacggtt aatacggtta ctgattcgtt tccccctgca tccgaatcac
    10681 gccgcacacc gcaaaaaacg cgcgcccgat gcgcaaccgc ttccgacatt tggcgcaatg
    10741 agacaaaacg gcaatatgcc gatacggata cgtcatcctc cgcctgcctg ttttttatat
    10801 catactcaat tttatctgtg cgttacgacg gcatgccgtc tgaagggtgt ttatgtctgc
    10861 atctaagaaa tttccgattc ctttgagcta tttcagcatc gcgctgggct tgtttgcctt
    10921 ggggctgtcg tggcgttacg gcgcgtctgt cgggctgctc cccgccttgg ccgccgaatc
    10981 gctgcttgcg gtggcttcgg ttgtctggct cttgctggtg gcggcatacc tgatcaaaat
    11041 gtttgcgtac cgaaacgatt ttttgtctga tttgcgcgac ttggtgcaat gctgcttcat
    11101 cagcgcgatt ccgattaccg ctatgctgga gggactcgcg ctgaagccct atcaggcagg
    11161 cgcggcggca gtcctgattt atgtcggcgt tgccggacag ttggcgtttt cgatgtatcg
    11221 ggcggccggt ctgtggcgcg gcctgcattc cttggaggcg acgacgccga ttatttatct
    11281 gcctacggtt gcgacaaact ttgtcagcgc gtcatctctg gccgcgttgg ggcatcatga
    11341 ttatgcagct ttgtttttcg gcgcgggtat gttttcctgg ctgagcttgg aagcctccat
    11401 cttgggcagg ctgcgcacgg cggcaccggt cggcacggcg gcgcgcggcg tggtcggcat
    11461 ccagcttgcg cccgcctttg tcggctgcgg cgcgtatttt gccgtcggcg gtaaagtcga
    11521 cggttttgcg ttggcattaa tcggctacgg ctgcctgcag cttttgttct tgctgcgcct
    11581 gacccgctgg ttttgggaag gcggttttac gatgagcttt tggggatttt cattcggttt
    11641 cgcggcaatg gcaggatgcg gtctgcatct ggcggcttct ggcatattgt cgggcttggg
    11701 gctgacgctt gccaccgccg gatcggcagg cgtggcgctg ctgcttgtcg gtacgctgca
    11761 ccggatagcg acggggcgtt tcttggtacg cggctgatgc gttttgccgc cttgtcaaaa
    11821 atgccgtctg aacgcccaag ccttcagacg gcatttttta tttcaaaccc ttacaggtag
    11881 aatttttcga ggactttcag gttgtcatcc aatttgtaca ccagcggctg accggtcggg
    11941 atttccaagc ccatgatgtc ttcgtcggaa ataccctcga tgtgttttgc cagcgcgcgc
    12001 agggagttgc cgtgcgccgc taccaagacg cgtttgccgc tcaaaatcgc gggggcgatt
    12061 tggtcttccc aaaacggcaa tacgcgatcc agcgttactt tcaggttttc gccgtcgggg
    12121 ataacatcgg caggcagatg ggcatagcgg cggtctttgt gtgcggaaaa cgcgtcgtct
    12181 ttgtccaaaa gcggcggcag agtgtcgtag ctgcgccgcc agatgcggac ttgctcgtcg
    12241 ccgtattgtt cggcggtttg ttttttgtcc aaaccttgca gttggccgta gtggcgttcg
    12301 ttcagccgcc acgttttgat ttgcggtacg aacagttggt cggattcttc caaaacgatg
    12361 ttgcaggtct taatcgcgcg ggtcaggacg gatgtgaagg cgatgtcgaa ctcatagccg
    12421 ttttctttca gttttttacc ggcggcggca gcctcggcaa gcccctgctc gctcagcttc
    12481 acgtcgcgcc agcctgtaaa caggtttttc gcgttccatt cgctttgtcc gtggcggata
    12541 aataccagtt ccatatcgtc tccaatgtgt gaaagtagga aagccttatt tataacatat
    12601 tttcacattt cccgtatttg attcagattt agacacggtg cccactatgg tttgccgttt
    12661 tgatttacaa taatgtcctt tgctttacat tccgcataca caatgtttca acacacagga
    12721 cgacacataa agcaccgccc tatgtgttgc cctgatttgg aaggggttgc cccctcccaa
    12781 ataaagtctg attctaccgc cccgaaggac agatgtccaa gtggcggggg ttcaaccgaa
    12841 aaggaaatac gataaatatg caaccgcacg ctccacatac cgattccaat acgctgatgc
    12901 tcggccgata cgccgaacgc gcctatctcg aatacgccat gagcgtggtc aaaggccgcg
    12961 cgctgcctga agtttcagac ggccaaaagc ccgttcagcg gcgcattttg tttgcgatgc
    13021 gcgatatggg tttgacggcg ggagcgaagc cggtgaaatc ggcgcgcgtg gtcggcgaga
    13081 ttttgggtaa ataccacccg cacggcgaca gttccgccta tgaggcgatg gtgcggatgg
    13141 cgcaggattt taccttgcgc tatcccttaa tcgacggcat cggcaacttc ggttcgcgtg
    13201 acggcgacgg ggcggcggcg atgcgttaca ccgaagcgcg gctgacaccg attgcggaat
    13261 tgctgttgtc cgaaatcaat caggggacgg tggatttcgt gccgaactac gacggcgcgt
    13321 ttgacgaacc gctgcacctg cccgcccgcc tgcctatggt cttgctcaac ggtgcgtcag
    13381 gcattgcggt gggtatggcg accgaaatcc catcacacaa tttgaatgaa gtcacacagg
    13441 cggcgattgc actgttgaag aagccgacgc tggaaaccgc cgacctgatg caatacatcc
    13501 ctgctcccga ttttgccgga ggcggtcaaa tcatcacgcc ggcggacgaa ttgcgccgta
    13561 tttacgaaac cggcaagggc agcgtgcgcg tgcgtgcgcg ttacgaaatc gagaagctgg
    13621 cgcgcggaca atggcgcgtc atcgtaaccg agctgccgcc gaacgccaat tccgccaaaa
    13681 tccttgctga aatcgaagaa cagaccaacc cgaaaccgaa agcaggcaaa aagcagctca
    13741 accaagacca gctcaatact aaaaagctga tgctggattt aatcgaccgc gtgcgcgacg
    13801 agtcggacgg cgaacatccc gtgcgccttg tatttgaacc aaaatccagc cgcatcgata
    13861 ccgatacctt catcaacacg ctgatggcgc aaacttcgct ggaaggcaat gtgtccatga
    13921 acttggtgat gatggggctg gacaaccgcc ccgcgcagaa aaacctgaaa acgattttgc
    13981 aggaatggtt ggatttccgc accgttaccg taacacgccg tctgaaattc cgtttgaacc
    14041 aagtggaaaa acggctgcac atcctcgaag gccgtctgaa agtctttctg cacatcgacg
    14101 aagtgattaa agtcatccgc gaatcagatg acccgaaagc cgatttgatg gcggcgttcg
    14161 ggctgaccga aatccaagcc gaagacattt tggaaatccg cctgcgccag ttggcacgtt
    14221 tggaaggttt caaactcgaa aaagaattga acgaattgcg cgaagaacaa ggccgtctga
    14281 acatcctttt gggcgatgaa aacgaaaagc gcaagctgat tatcaaagag atgcaggcgg
    14341 atatgaaaca atacggcgat gcacgccgca cgctggtgga agaggccgga cgcgccgtgc
    14401 tgacacaaac caccgccgac gaacccatca cactgattct gtcggaaaaa ggctggatac
    14461 gcagccgtgc cggacacaat ctcgatttga gccaaaccgc gttcaaagaa ggcgaccgcc
    14521 tcaaacaaac ccttgaaggc agaacggttt tacccgtcgt catcctcgat tcattgggca
    14581 gaacctatac actcgatgcc gccgaaattc ccggcggacg cggcgacggc gtgccggttt
    14641 catccttgat cgagttgcaa aacagcgcga aacccgttgc gatgttgaca ggattgccgg
    14701 aacaacatta tttattatca agcagcggcg gctacggctt catcgccaag ttggctgata
    14761 tggtcggacg cgtaaaagcg ggcaaagtgg tgatgaccac agacagtggc gaaaccgtcc
    14821 tgccgccgat tgccgtctat gcctcctcgt tcatcaaccc cgactgcaaa atcattgccg
    14881 ccaccagtca aaaccgcgcc ctcgccttcc ccatcggcga attgaaaatt atggcgaaag
    14941 gcaaagggct gcaaatcatc ggattaaacg ccggcgaatc gctgacgcat accgccgttt
    15001 cttccgagcc ggaaatcctg attgaaagcg aaggcaggcg cggcgcggcg cacaaagacc
    15061 gcatccccgt ctccctgctt gaggcaaaac gcggcaaaaa aggcaaacta ttacccatat
    15121 cgggcagcct gaaacagctt tcttccccca aataaacccg attccacaca taattatggt
    15181 gatttccaac ccccgcgaac ttgaaaaact caaagaccgg attcccaatc tgatcaacat
    15241 catccgcgtc gccatcgttt ttccgctgat gattatgcac atcctcgggc tggaaaccgg
    15301 cagccgtgcg aacctgcacg cttcgtggac ggcgtgggcg ttttatgttt ggctcgccat
    15361 tgcctgctgg ctgattttct tttccatcat ccatccgcat tggcaatggc agtcgctgaa
    15421 aatgccgcgt ttcagcgcgg tggcggacat cacgatgatc ggcgtgctga cctacctgtt
    15481 tggcggcatc gattccggct tcggcatcct gatcctgccc ttcgtcgtct gctcctgcct
    15541 gctcagctac gggcgttacc ccctgctcta ttccagctac gccgccatcc tgctgatatt
    15601 caacgccatt gccgacggcg atatcggcaa atacccgctc atatcggatg cccgaaccgc
    15661 ctcggtaacc ttcatccttg tcgccgcctc ctatctttcc gccatcttca cctcactgtc
    15721 ggtcagatac atcgacagcg ccggccggct ggcacgcgac aaccacctcg cctaccggcg
    15781 catcaaaggc ttgagccaaa ccgtactcaa ccgcgttcag gaagcggtcg tcgtcatcaa
    15841 cgtcgagcat cagaccatac tgttcaataa aaaggcaaaa gacctgctac ccatgcttga
    15901 aatcggacag catactgccc tgttcgaccc tattaccgtt ttatgggaca aggcctcttc
    15961 gcgtactttc gaacgcaata tcgacacgcc cggcctgaac gccggcatcc gcgccgttcc
    16021 ggtcaacaaa gggccggaca agctgctcat cctctacatc cgcccgcaaa gcgaaattca
    16081 ggcagaagcc ctgtccgtca aacttgccgc gctcggacaa ctgaccgcca acctcgccca
    16141 cgaaatccgc aacccgatgt ccgccatccg ccacgccaac gacctgctgc gcgaaaatat
    16201 ggaagcgggg gcggcagatc cgttcaacgc caaattgtgc aaaatcatcg acggcaacgt
    16261 ctgccgtatc gacaaaatgc tcgaagatat ttcctcgcca acaagcgcaa caaaaccgaa
    16321 cgcgaaacca tcggcctgat accgttttgg gaagaattca aacaagagtt cctgctcggc
    16381 catcccgatg ccgccggctg catccgccta gatatgcagg gcaatcacct gactgcctat
    16441 ttcgatcctg cccacctgcg gcaaattatg tggaacctcg ccaacaacgc gtggcggcac
    16501 agcagcaaac gtaacggctc gattgccgtc gtcgcccgtc ctgcacaaaa aaacaccatc
    16561 agcatcctgt ttgccgacga cggcggcggc gttccgcccg aagtgcagga acacttgttc
    16621 gaaccctttt acaccacagc aaaaaacgga accggcttgg gactgtatgt cgcccgcgaa
    16681 ctggcacacg ccaatttcgg cgatttgacc tacctgccgg aagccaaatg tttcgaactc
    16741 acattaccgg aaaaaaccaa tgactgaact gcaacatccc gtcctcgtcg tcgatgacga
    16801 aaccgacatt ctcgacctga tggaaatgac cctgatgaaa atgggcttgc gcgtccatac
    16861 cgcgtcaggc gttgccgaag ccaaaaacaa gctcgacagc caacgctatt cgctcgtcct
    16921 gaccgatatg cgtatgccgg acggctcggg gctagaagtc gtccaacaca tcaacagccg
    16981 cctgctcgat acgccggttg ccgtcatcac cgccttcggc aatgccgatc aggcacagga
    17041 agccctgcgt tgcggcgcgt tcgaccccga taccatgcag atacaggact atctcgacca
    17101 aatcgaacgc gacatcatcg aacaaaccct caaacaaacc gaaggcaacc gcacgcaggc
    17161 cgacaaacgc ttgggcatca gcttccgttc catgcgctac cgtatggaac gcctcaacat
    17221 cggctgacga caaaacggca tccgcaccat ctccgcccac ccgaaaaaat gccgtctgaa
    17281 acggcacggg aaagcgggtt cgccccacgc ccgaacggac acaaaacacc atgaccgaca
    17341 tccttattga caacaccgcc accgaaaccg tccgcaccct gatacgggca ttcccccttg
    17401 tgcccgtttc ccaaccgccc gaacaaggca gctacctcct taccgaacac gataccgtca
    17461 gcctcaggct tgtcggggaa aaaagcagcg tcatcgtcga ctttgcctcg ggagccgcac
    17521 aataccggcg cacaaaaggc ggaggagaac tcatcgccaa agccgtcaac cataccgcca
    17581 aacccaccgt atgggatgca accgcaggat tggggcgcga cagcttcgtc ctcgcctcgc
    17641 tcggactgac cgttaccgcc ttcgagcagc atcccgccgt cgcctgcctg ctttcagacg
    17701 gcatccgccg cgccctcctc aatcccgaaa cgcaagacac cgccgcgcgc atcaacctcc
    17761 atttcggcaa cgccgccgaa cagatgcccg cccttgttca aacacaaggc aaacccgaca
    17821 tcgtctatct cgaccccatg tatcccgaac gccgcaaaag tgccgccgtc aaaaaagaaa
    17881 tggcctactt ccaccggctc gtcggcgagg cgcaagatga ggccgtcctc ctccataccg
    17941 cacgccaaac ggcaaaaaaa cgcgtcgtcg tcaaacgccc ccgcctcggc gaacaccttg
    18001 ccggacaagc ccctgcctac caatacacag gcaaaagcac ccgcttcgac gtttacctgc
    18061 cctacgaagc ggacaaggga taacgcccat aaaacaagac accgaaaaat ttgccgttct
    18121 tatgcaaacg agaaaccggt ttttgcgttt cgactgtttt ggataaatca tcacacctta
    18181 aagtttgtca ttcccacgga agtgggaatc cagaaatgaa aagcaacagg aatttatcgt
    18241 aaatgaccga aaccgaacgg actagattcc cgcctgcgcg ggaatgacgg ggtgggagga
    18301 tgccgtctga aattccgtca ttcccgtaaa aacgggaatc tagaacttct gatttttcag
    18361 acgacttttg aacattgccg ctacccaatg atctggattc ccgcctgcgc gggaatgacg
    18421 atgtaaaatt atccgggatt caaaaagaca gactttcgcg tccgtgggaa tgactgcgga
    18481 aagatgattt ttattttgtc ataaaaatcc gcaccttaat cagttggcgg ttaaatcaaa
    18541 cttttagggt gcagattact ttttatgatt tcagacagca ttttgacaga ccgcaaacct
    18601 atttcggcaa taccaaaaac ttaatcaata actctttgaa cacaaaaccg aacacgccca
    18661 agcccaaaac caaaaacaaa atggcgatgc cgaatttgcc tgctttggac tccttgccca
    18721 aattccaaac gataaaaccc aaaaaaataa tcaagccggt caggcagatt ttcaacgccc
    18781 aatcggcaaa aactgcttca tccatatttt ttcctattgt tgatgtgtat gccatacgcg
    18841 ataagggttt cagacggcat ctgctgtcca atgccgtctg aaacacgcaa tcagcgcgcg
    18901 agtgcctgtt tcaaatcgtc aatcaaatcg ccaacatatt ccaaaccgac cgacaggcgc
    18961 accaatccgg ggcggatgtt ggcggcgagt ttttcttcgg gctgcatcct gccgtgcgtg
    19021 gttgtccacg gatgggtgat ggtcgagcgc acgtcgccga ggttggcggt acgggaaaag
    19081 agttccacgc cgtccacgac tttccacgcg gctgcttggt cggcaacttc aaagccgatg
    19141 acgatgccgc cgccgttttg ctgtttgcgg ataagcgccg cctgcggatg gtcgggcaat
    19201 ccggtgtagt acacggcttg aacctgcggc tgcgcttgca gccattgcgc gattttcagg
    19261 gcgttgtcga actgtttttc catacgcagc gacagggttt ccacgccgct caacaactgc
    19321 cacgcattaa acggcgacat tgccagcccg caagagttgc aatacatagc gacctgcgcc
    19381 atcagctctt ccgaacccgc caacacgccg cccatcacgc gcccgtgtcc gtctatggct
    19441 ttggtcgcgg aggaaacgga aatatccgcg ccgtgtttca aaggctgcga gccgacgggc
    19501 gacagcaggc tgttgtccac caccaaaatc gcgccgatgc cgtgcgccaa ttccgccaag
    19561 gcttccaagt cggccacttc gcccaaggga ttggacggcg tttccaaaaa cagcagtttg
    19621 gtgttggctt tgacggcggc tttccattcg tttatatcag tcggcgacac gtggctcact
    19681 tcgatgccga atttggtaac gatgttattg ataaagccga cggtcgtgcc gaacaggctg
    19741 cggctggaaa ccacgtggtc gcccgcctgc aaaaaggtga aaaacgccgc ctgaatcgca
    19801 gacatgccgg tcgaagtggc gaccgcgcgt tccgcacctt ccaaagcggc gatgcgtttt
    19861 tcaaaggcgg cggtggtcgg gttggcggta cgggtataag tgaagccttt gatttttttt
    19921 gaaaacaaat cggcagcgtg ttgggcgttg tcccacatga aactgctggt caggaacaat
    19981 gcctgattgt gttcgcggta tccggtttgt tctttgccgc cgcgtatggc gagcgtttgc
    20041 ggatggagtt ttttgctcat cggtgattcc tcggttttgt ccgttcaaaa acggggcgtg
    20101 cgcccgttgt ttaatttgtt aatattttgc gcctgttcta tgatgctttc aagtcgggtg
    20161 agaatgcaaa tgccgtctga aacggctttc agacggcatg gcaatcagcg tttgtatttt
    20221 aactcgtact tgatgtcgtt gaggattttg cggacatcgt gttccaacac gtcttcgact
    20281 accgcacccg cctgctcgtg cagcatctgc tggagctgat aggtaaaaac cgccatctgc
    20341 ttttgcaccg ccgtacggat gataccgttg acggtatcgg tcagatgcgg gcgcaggcgt
    20401 ttgatcagcc gttcggtcag ctcctgttcg gacaggcaga acacttcgcg ccggttgacg
    20461 gctttcgggt tcaggatatt gagttggacg ggcatcaacg tttcttccgc atcgttttcc
    20521 ccgttttccg aaaccgccgg ctcattcgtg ccggattctg cctcgtcggc gttttccccg
    20581 ctttcaatct gtccggtttc aaattcgaca ctgtcttttt tggtatcaaa ccggattctc
    20641 cgccgcgatt cgatgtgttt ttccgaaacc gacatttgca gggaagcctg cgcgttgagc
    20701 cagttttcct gaaggacgat catcgggtcg gtttcgactt cctcgccgca atcggcaacg
    20761 gcggcattgt gttcctcctg ccattttttc agatacgcct tcaacacacg ggctcggctc
    20821 tcatcgtcca gtttcggcac aggcgcgtcc gttccggttt cagaggggcg ggacagcggc
    20881 gcgtaagtcg gcactgcctt catacggcgc gtctgacgca ggttttccaa acgtttttcc
    20941 caattcggct ctttattcgc atccattttc ggcttccggt tcttaatctt tgcaagcaga
    21001 caaacccgcg cccaaagcgc ggtttgatat aatggcgcat tttaacagat tcgcgaggat
    21061 acatcatggg cagcatcgaa cagcgtttgg aatatctgga agaggcgaac gacgtgctgc
    21121 gtatgcagaa ccacgtcctg tccaccgcat tcaaagcctt aatccgcgcc cttcccgccg
    21181 aaaccgccga aatcgcggtc gagtcgattc agcttgcttt tgaggacgcc ttggcagaat
    21241 tgagctatga ggacagcccg catacggatt tgttccacga cgttacttat gcgtttttcc
    21301 gtgaaaaaga acgttaattt tatgttaaac tgatttttta ggctttttga ttaccgaaag
    21361 gaattttgat gaatatgaaa aaatggattg ccgccgccct tgcctgttcc gcgctcgcgc
    21421 tgtctgcctg cggcggtcag ggcaaagata ccgccgcgcc tgccgccaac cccgacaaag
    21481 tgtaccgcgt ggcttccaac gccgagtttg ccccctttga atctttagac tcgaaaggca
    21541 atgtcgaagg tttcgatgtg gatttgatga acgcgatggc gaaggcgggc aattttaaaa
    21601 tcgaattcaa acaccagccg tgggacagcc ttttccccgc cttaaacaac ggcgatgcgg
    21661 acgttgtgat gtcgggcgta accattaccg acgaccgcaa acagtctatg gacttcagcg
    21721 acccgtattt tgaaatcacc caagtcgtcc tcgttccgaa aggcaaaaaa gtatcttctt
    21781 ccgaagattt gaaaaacatg aacaaagtcg gcgtggtaac cggctacacg ggcgatttct
    21841 ccgtatccaa actcttgggc aacgacaatc cgaaaatcgc gcgctttgaa aacgttcccc
    21901 tgattatcaa agaactggaa aacggcggct tggattccgt ggtcagcgac agcgcggtca
    21961 tcgccaatta tgtgaaaaac aatccgacca aagggatgga cttcgttacc ctgcccgact
    22021 tcaccaccga acactacggc atcgcggtac gcaaaggcga cgaagcaacc gtcaaaatgc
    22081 tgaacgatgc gttggaaaaa gtacgcgaaa gcggcgaata cgacaagatt tacgccaaat
    22141 attttgcaaa agaagacgaa caggccgcaa aataagcccg cccgtccgaa cacaatgccg
    22201 tctgaagccc tttcagacgg cattgttcat caatcggcct acaatgaact gcctgctgat
    22261 ttctccctac cgcaaagcaa caggcaaaga ttacaaatat caaaatccga gtaaaacagt
    22321 attttattaa aacaaattga taatcaagag attagaatta tgtattgtct ttaccgtaca
    22381 aacgctggca ctatttcaac ctgataaaaa acagccttca aaaaggttgc ttaaaacagc
    22441 agcagacact taccgccaca accttgaaaa ggaacacaat catgaccgtc atcaaacagg
    22501 aagactttat ccaaagcatt tgcgatgcct tccaattcat cagctactac catcccaaag
    22561 actacatcga cgcgctctat aaagcgtggc agaaagaaga aaaccccgcc gccaaagacg
    22621 cgatgacgca gattttggtc aacagccgca tgtgtgccga aaacaaccgc cccatctgcc
    22681 aagataccgg tatcgcgacc gtcttcctca aagtcggtat ggatgtgcag tgggatgcgg
    22741 acatgagcgt cgaagaaatg gttaacgaag gcgtgcgccg cgcctacact tgggaaggca
    22801 atacgctgcg cgcttccgtt ctcgccgacc ccgccggcaa acgccaaaat accaaagaca
    22861 acacgcccgc cgtcatccat atgagcatcg tgccgggcga caaagtcgaa gtaacctgcg
    22921 cggcaaaagg cggcggttct gaaaacaaat ccaaactcgc catgctcaat ccttccgaca
    22981 acatcgtcga ttgggtattg aaaaccatcc cgacgatggg cgcgggctgg tgtcctcccg
    23041 gcatcttggg catcggcatc ggcggcacgc ctgaaaaagc cgtgttgatg gcaaaagagt
    23101 ccctgatgag ccacatcgac atccaagaat tgcaggaaaa agccgcgtcc ggcgcggaat
    23161 tgtccaccac cgaagccctg cgcctcgaac tctttgaaaa agtcaacgcg ctgggcatcg
    23221 gcgcacaagg tttgggcggt ctgaccaccg tgttggacgt gaaaatcctc gattacccga
    23281 cccacgccgc ctccaaaccg attgccatga ttccgaactg cgccgccacc cgccacgtcg
    23341 aatttgaatt ggacggctca ggccctgtcg aactcacgcc gccgcgcgtc gaagactggc
    23401 ccgatttgac ttacagcccc gacaacggca aacgcgtcga tgtcgacaag ctgaccaaag
    23461 aagaagtggc aagctggaaa accggcgacg tattgctgtt gaacggcaaa atcctcaccg
    23521 gccgcgatgc cgcacacaaa cgcctcgtcg atatgctcaa caaaggcgaa gaattgcccg
    23581 tcgatttcac caaccgcctg atttactacg tcggccccgt cgatccggtc ggcgatgaag
    23641 tcgtcggtcc ggcaggtccg accacagcca cccgcatgga caaattcacc cgccaaatgc
    23701 tcgaacaaac cggcctcttg ggcatgatcg gcaaatccga acgcggcgcg gcggcgtgcg
    23761 aagccatcgc cgacaacaaa gccgtgtacc tgatggcggt cggcggcgcg gcatacctcg
    23821 tggcaaaagc catcaaatct tccaaagtct tggcgttccc cgaattgggt atggaagcca
    23881 tttacgaatt tgaagtcaaa gatatgcccg taaccgtcgc cgtagacagc aaaggcgaat
    23941 ccatccacgc caccgccccg ccccaatggc aggcgaaaat cggcatcatc cccgtcaaat
    24001 cttgaggcgt catgccgtct gaacacaaaa tctgctttca gacggcattt ccgcccccgg
    24061 ttgcggtaca atccaccatt tcatcactcg gcgacccaca ccgtgaaaat cctcatttta
    24121 ggcaacggac aggtaggttc taccgtcgca caaaaccttg ccgccatacc caacaacgac
    24181 gtaaccgtta tcgacatcga cgaaaaagca ttgcaggaaa caggcagccg cctcgatgtc
    24241 caaaccgttt tcggcaacgg cgcatccccc ttcacattag aacgcgccgg cgcggaagat
    24301 gccgacttgc tgctcgcgct ctcccgcagc gacgaaacca acatcgtcgc ctgcaaagtt
    24361 gccgccgacc tgttcaacat ccccggccgc atcgcgcgcg tccgttccag cgaatacctc
    24421 gaatacctca gccccaagct cgaaaacaac gaaaacggca gcctttccat attcggcata
    24481 accgaaacca tcagccccga acagctcgtt accgaacagc ttgccggcct gatagactgc
    24541 ccgggcgcat tgcaggtttt acgttttgca gacgaccgcg tgcggatggt catcatacag
    24601 gcgcggcgcg gcgggttgct tgtcggacgc agcattgccg acatcgccca agatttgccc
    24661 gacggggccg actgccaaat ctgcgccgtt taccgcaaca accgcctcat cgtccccgcg
    24721 ccgcaaaccg tcatcatcga aggcgacgaa atcctatttg ccgccgccgc cgaaaacatc
    24781 ggcgcggtca tacccgaatt gcgccccaaa gaaaccagca cccgccgcat catgattgcc
    24841 ggcggcggca acatcggcta ccgtctcgcc aagcagctcg aacacgcata caacgtcaaa
    24901 atcatcgaat gccggccgcg ccgtgccgaa tggatagccg aaaacctcga caacaccctc
    24961 gtcctgcaag gttcggcaac cgacgaaacc ctgctcgaca acgaatacat cgacgaaatc
    25021 gacgtattct gcgccctgac caacgacgac gaaagcaaca ttatgtccgc ccttttggcg
    25081 aaaaacctcg gcgcgaagcg cgttatcggc atcgtcaacc gctcaagcta cgtcgatttg
    25141 ctcgaaggca acaaaatcga catcgtcgtc tccccccacc tcatcaccat cggctcgata
    25201 ctcgcccaca tccggcgcgg cgacatcgtt gccgtccacc ccatccggcg cggcacggcg
    25261 gaagccatcg aagtcgtcgc acacggcgac aaaaaaactt ccgccatcat cggcaggcgc
    25321 atcagcggca tcaaatggcc cgaaggctgc cacattgccg ccgtcgtccg cgccgaaacc
    25381 ggcgaaacca ttatgggaca ccataccgaa accgtcatcc aagacggcga ccacatcatc
    25441 tttttcgtct cgcgccggcg catcctgaac gaactggaaa aactcatcca ggtcaaaatg
    25501 ggctttttcg gataaaccgc cccattccgg acacattgcc gccaagcggt atggaagcgg
    25561 aaataatggt aggtgggctt cagacggcat ccgccctccc cgtccttccc gcgtaagcgg
    25621 gaatccagac cttgggatag cggcaatatt caaaggttat ctgaaaattt agaggttcta
    25681 gattcccgtt tccacgggaa ggacgaagag ttgcgggaat ctagaccatt ggaaagcggc
    25741 aatattcaaa ggttagctga agctttagag gttctagatt cccgtttcca cgggaatgac
    25801 gaaaggtggc gggaatccaa accattgggc aacagcaata ttcaaagatt atctgaaagt
    25861 ttgaggttct agattcccgc tttcgcggga atgacgaaaa gtggcgggaa tgacgaagtt
    25921 tcagacggca tcgcccgcct gttttgatat ggcggcaccc cccccgacaa aaaaaacaat
    25981 ccggaacgca tctgaccgtt ccggcttgtt ttcaggcgaa tccgccgcat cagaacatac
    26041 tgcgcacgcc catattgacc tgccaagtct ggcgcatcgt gtgcatcgaa gacctttgcg
    26101 cctcaaaata aagctgcctt ccgttgtcgg cattaccacg caaaaaaaat gaattgcttg
    26161 atattccaat gttttttata tgtttttata ttgtgatgcg atcagacaaa cgcccccctg
    26221 acatttgttt agacggcatc gtattgctaa atttctataa gtatgtataa tgtccgtttc
    26281 cacgcgccca tcgtctagag gcctaggaca ctgccctttc acggcggcaa ccggggttcg
    26341 aatccccgtg ggcgtgccaa ttcaaaaacc tgcttgtttc aagcaggttt ttttattatg
    26401 aatcgtcatt cccgcac
//
LOCUS       DAXHMX010000010        24869 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHMX010000010 DAXHMX010000000
VERSION     DAXHMX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.010"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(91..1038)
                     /locus_tag="ACFM4S_000314"
     CDS             complement(91..1038)
                     /locus_tag="ACFM4S_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6050069.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(1053..1460)
                     /locus_tag="ACFM4S_000315"
     CDS             complement(1053..1460)
                     /locus_tag="ACFM4S_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6050070.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHAGGNRYEVFYRGT
                     HWQAQNTGQDVFEPGTRALIVRKEGNLLIITHP"
     gene            1541..2200
                     /gene="ung"
                     /locus_tag="ACFM4S_000316"
     CDS             1541..2200
                     /gene="ung"
                     /locus_tag="ACFM4S_000316"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6050071.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAEDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            2361..2759
                     /locus_tag="ACFM4S_000317"
     CDS             2361..2759
                     /locus_tag="ACFM4S_000317"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6050072.1"
                     /translation="MIDVTDFNDEDKLGKGKEMIDRLSRLVGIFKKLNLSSNQAEDDD
                     LLGDAYEYLMRHFATESGKSKGQFYTPAEVSRIMAKIIGISADCRPAPAFMTRPAARV
                     RCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            2672..3305
                     /locus_tag="ACFM4S_000318"
                     /pseudo
     CDS             2672..3305
                     /locus_tag="ACFM4S_000318"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            3653..4597
                     /locus_tag="ACFM4S_000319"
     CDS             3653..4597
                     /locus_tag="ACFM4S_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6050073.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            4748..4873
                     /locus_tag="ACFM4S_000320"
     CDS             4748..4873
                     /locus_tag="ACFM4S_000320"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050074.1"
                     /translation="MLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT"
     gene            complement(5163..5678)
                     /locus_tag="ACFM4S_000321"
     CDS             complement(5163..5678)
                     /locus_tag="ACFM4S_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050075.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(6144..8066)
                     /locus_tag="ACFM4S_000322"
     CDS             complement(6144..8066)
                     /locus_tag="ACFM4S_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6050076.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQTLQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(8143..8514)
                     /locus_tag="ACFM4S_000323"
     CDS             complement(8143..8514)
                     /locus_tag="ACFM4S_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6050077.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYLEILIIGTGAAQEFIHPKIMADFSRIGIGVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            8711..10018
                     /locus_tag="ACFM4S_000324"
     CDS             8711..10018
                     /locus_tag="ACFM4S_000324"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6050078.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            10011..10445
                     /locus_tag="ACFM4S_000325"
     CDS             10011..10445
                     /locus_tag="ACFM4S_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050079.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKSE"
     gene            complement(10504..10773)
                     /locus_tag="ACFM4S_000326"
     CDS             complement(10504..10773)
                     /locus_tag="ACFM4S_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6050080.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(10955..13417)
                     /gene="lon"
                     /locus_tag="ACFM4S_000327"
     CDS             complement(10955..13417)
                     /gene="lon"
                     /locus_tag="ACFM4S_000327"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6050081.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELLVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLASDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNFKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAVAESASKPKPRSRATKH"
     gene            13668..14771
                     /locus_tag="ACFM4S_000328"
     CDS             13668..14771
                     /locus_tag="ACFM4S_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6050082.1"
                     /translation="MSSFIFPDNGAEWRNESVQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRARKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMM
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(14799..16544)
                     /gene="recD"
                     /locus_tag="ACFM4S_000329"
     CDS             complement(14799..16544)
                     /gene="recD"
                     /locus_tag="ACFM4S_000329"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6050083.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAIRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPLDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSHKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(16612..17307)
                     /gene="lolD"
                     /locus_tag="ACFM4S_000330"
     CDS             complement(16612..17307)
                     /gene="lolD"
                     /locus_tag="ACFM4S_000330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6050084.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEILAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKKREEAAETAMAMLEKVGLKHRSAHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(17300..18547)
                     /locus_tag="ACFM4S_000331"
     CDS             complement(17300..18547)
                     /locus_tag="ACFM4S_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6050085.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            18752..19030
                     /locus_tag="ACFM4S_000332"
     CDS             18752..19030
                     /locus_tag="ACFM4S_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050086.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(19086..19703)
                     /gene="recR"
                     /locus_tag="ACFM4S_000333"
     CDS             complement(19086..19703)
                     /gene="recR"
                     /locus_tag="ACFM4S_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6050087.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVHHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEVANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(19770..21308)
                     /locus_tag="ACFM4S_000334"
     CDS             complement(19770..21308)
                     /locus_tag="ACFM4S_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6050088.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLLGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKLSFEQEKAAVENELKMKKAVADFN
                     KAKEKLSDDAFNHPASLAEAAKNSSLKVETQETWLSRQDAQMSGLPENLINAIFSDDV
                     LKKKHNSEVLSINNEIAWVVRAKEVREEKTLPFAEAKDAVRQAYIRAEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPTNGKPAYVRLTGLP
                     APVIVEVQAITPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GGGQ"
     gene            complement(21362..21736)
                     /locus_tag="ACFM4S_000335"
     CDS             complement(21362..21736)
                     /locus_tag="ACFM4S_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6050089.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQMPSEAEICS
                     WLEQVPLETLLNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            21886..23514
                     /locus_tag="ACFM4S_000336"
     CDS             21886..23514
                     /locus_tag="ACFM4S_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6050090.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGSRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            23599..24852
                     /locus_tag="ACFM4S_000337"
     CDS             23599..24852
                     /locus_tag="ACFM4S_000337"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6050091.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYAGFSFHTDKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         5763 a         6707 c         6459 g         5940 t
ORIGIN      
        1 tttgaagcgg aatacaacct tgcccgggtt taaataaaaa tgccgtctga accccgaaaa
       61 cagagcttca gacggcattt tcatcacggc ttatttggcg gttttgctgc tgtcgataat
      121 tttcataccg gcagaaatca ggctgccgat gtcggcaaca ttggcgggca taatcagcgt
      181 attgctttct ttggcaagat tgttgaacgc agcgacgtat tgttccgcaa tcttcagatt
      241 gaccgcatcc gcgccgcctt gggtttgaag ggcggcggca atttgacgga tggcttcggc
      301 attggcttcg gcaacaaggc gcagggattc cgcttcgcct ttggcgcggt tgatgcgggc
      361 gattttctcg gcatttgacg cattgaccgc ggcctgggcc tcgccttcgg attgttggat
      421 ttcggcttcg cgctgaccac tggcaaggtt gatttgttcg attttacggc cttcggattc
      481 ggcgatacgg gcgcgttttt cgcgttcggc agtaatttgc gcctgcattg agcgaaggat
      541 ttcttgcggc ggaaccaaat ccttgatttc gtaacggaga actttcacac cccaagctcc
      601 ggcggcttca tcgagggcgg aaacgacggt gctgttgatt tcgtcgcgtt cttcaaacgt
      661 tttgtccaac tccatacgcc cgataacgga acgcagcgtc gtttgggcaa gctgggtaat
      721 cgccataatg tagttgctcg aaccgtatga ggcgagtttg ggatcggtta cttggaaata
      781 gatgatgccg tcaaccgtca gctgggtgtt gtcgcgcgtg atgcagacct ggctgggtac
      841 gtctaaaggg atttctttca gcgaatgacg gtaggcgacg cggtcgataa agggaatcaa
      901 aatattcaaa ccggccgtca aggcgcgatg gaaacgtcca agcctttcga caacgtggac
      961 ttcctgttgt gggatgacaa caaaggattt gaaaccgaaa acggcgacgg ctaccaacaa
     1021 gataatgaaa aattccataa ttcctccgag tgttaagggt gtgtgataat aagaaggttg
     1081 ccttccttgc ggacaatgag ggcgcgcgtt cccggttcaa acacatcctg ccccgtattt
     1141 tgcgcctgcc agtgcgtacc gcgataaaaa acttcgtaac ggttgccgcc tgcgtgccgg
     1201 aggatttcgg catattgccc ggcatccaaa tcctgatatg aatccgtttc aacttttccc
     1261 acggcggtct tggcgtgtac gaaccaaata cccagcgcgg aaagcagagc ggcggtcaag
     1321 acggcggcag gcgtgctgcc ggtcagcccg taagcaatgc ccgaacccgc caaagccgcg
     1381 ctgacaacca aaagataaac cgttcccgtc aataattcga tgattaagac ggcaacagcg
     1441 gcaacaaacc atacagtcat acatttcccc acaaagcgcg tcgtttgaca aaatcacgcg
     1501 atatcagcag tatagccgaa tttgaaagga tagggcagat atggatactt ggcacgatgc
     1561 actgggcaac gaaaaacggc aaccgtattt tcaggaaatt ttaaatgcag tcaggcagga
     1621 acgtttgtcg gggcaaatca tctatccgcc ggcggaggat gtgttcaacg cattccgcct
     1681 gacagcgttt gaccgagtca aagtcgtcat tctcggacaa gacccgtatc acggggcagg
     1741 gcaggcgcac ggtttggcat tttccgtcag gcagggtgtc cgcataccgc cgtctttact
     1801 caatatctac aaagagttgg aaaccgacat caacggtttt tccattcccg atcacggctg
     1861 cctgacggct tgggcggagc agggcgtatt gcttctgaac acggttttga cggtgcgtgc
     1921 aggacaggcg cattcgcacg cccttttagg ctgggaacgc tttaccgata ccgtcatcag
     1981 gcagcttgcg gcgcaccgca agcaccttgt cttcatgttg tggggtgggt atgcacaaca
     2041 aaaagggagg ctgatagaca gtcaaaatca tttgatattg accgcaccgc atccgtctcc
     2101 tctgtcggca tatcgcggtt ttttcggctg ccgccatttt tcacaggcaa acagctattt
     2161 gagtcaacac ggtatcgaac cgataaactg gaagctatga atgccgatat agccgttgcc
     2221 gccggcgtgt taaaatcgcg attgagctgc acgccggtac gacttttgac gacatcgtca
     2281 aactcaaaaa caccgccgac atcggcgacc gcctgaataa gattatcgcc caaattgccg
     2341 aagccaacga cttaaaaggc gtgatcgacg ttaccgactt caacgacgaa gacaaactgg
     2401 gtaaaggtaa ggagatgatc gaccgtttga gcaggcttgt cggcattttt aaaaagctca
     2461 acctttcttc caaccaagcc gaagacgacg atttgttagg tgatgcctac gaatacctga
     2521 tgcgccattt tgcgaccgag tcaggcaaat ccaaagggca gttttacacg cctgccgaag
     2581 tctcccgcat tatggcgaag attatcggaa tcagcgcaga ttgccgtcca gcaccagcgt
     2641 ttatgacccg acctgcggct cgggttcgct gttgctcaaa gccgccgccc aagccggcag
     2701 ccaaatcagc ctttacggtc aggaaaaaga tgtggcaacc gcgtcccttg cccgtatgaa
     2761 tatgattttg cacaacaacg aaaccgccga aatcaacacc gggaacacct tgtccgattc
     2821 gtctttccgt gatgaaaacg acgggcttaa gaccttcgat tttgccgttg ccaatccgcc
     2881 ttatcccgcc cgaaaaaaac ggcgattacg cctttttgct gcatctgctc aaaagcctga
     2941 aaccaagcgg caaaggtgcg attattcttc cgcacggtgt gctgtttcgc ggcaatgccg
     3001 aagcgcgtat tcgcacggaa ttgcttaacc ttgaccttat taaaggcatt atcgggctgc
     3061 ctgccaacct gttttacggc acgggcattc ctgcctgcat catcgtcatc gacaaagaac
     3121 acgcccaaac cgcccaattt gccgaagagg gaacaaacca agttatcagc ggcggcagcg
     3181 tgtttatgat tgacgcatcg cgcggcttca ttaaagacgg caacaaaaac cgtctgcgtg
     3241 agcaagacat tcacaaaatc atcgacactt tcacaaacct cgttacagcc gtatggtgca
     3301 tttaagcgaa atcgcagcac aagattacaa ccttaatctg cctcgctata tcgacagcgg
     3361 agaagtcgaa gacctgcaaa atctcgccgc ctatctatct ttatggcggc atacctgcgc
     3421 acgatatgga cgcattggaa gcctattggc aagttttagg ccgtatgaaa aacgagttgt
     3481 ttgccgaaca cgatggccac tttaccacta tacaacggaa tcgattgcaa atctttccca
     3541 ctctcaacag cttaaaatcc tgcgggattg gtgtggaatt tagggctaat ctagtacagc
     3601 cccaaattta atccactata aaatcgaaag cagccaaatc aaagcccata tattggcgca
     3661 ccccgattac gccgccttca aagccggaca cctggcaaag tttgccgcgt ggcacactca
     3721 aaacgacctt gccgccatcc aaccgggcag gcttatccgg aaatggagcg aaagcctgct
     3781 ggacgcgttc aaacccggca gcctgattga agaatacgat ttctaccaaa tcctgacgga
     3841 ctactgggcg gaaaccctgc aagacgatgt ttatctcatc gcccaagatg gctggaaggc
     3901 ggttaaaaac ctggccgaaa tcaccaaaga aagcgatgaa gccgcgaacc tgaccgtcgt
     3961 ctttgaggaa accgaaaccg acaaaaaagg caaagccaaa accaagcgca tcagcaaaaa
     4021 ataccgcagc gaagtcatcg cccccgagct ggttgcccgc cgctactttt cagacggcat
     4081 cgccaagctg gaagaaaaac aaagcgagct ggaacgccta agccaagaat tggaaaacca
     4141 catagaagaa cacggcggcg aagagggtgc gctgaacgac gtattggatg caaaaggcaa
     4201 actttccgcc aaacttctga aaaccgcatt ggaagaaagc ggcatagaag aaggcgaacg
     4261 ggctgtttta caaaccaccc aaacactgat gacgcaggaa aaagccgcga aagacgcagt
     4321 caaaacccaa atcgaagccc tgaaccttgc cgtattcaaa caattcggcc gactttccga
     4381 agccgaaatc aagcagcttg ccgttcaaga caaatggctt gccgatttac aaagtcgaat
     4441 cgaaaatcgc ttggaaaaca gtattcagca gcttatcagc cgcttgaaca cgctggaaga
     4501 ccgctaccgc agcccgatgg ccgagcttgc ccgagaagtg gaaaagtggc aaagcaaagt
     4561 caatgcccac cttgaaaata tgggttttgg aggctgaaat ggcggcacag acaggctata
     4621 aggcgagcgg gttttgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag
     4681 acatttaggg gatcgcggtt cgggtgtccg caccgcttaa tacgtcgtcg tccacgaact
     4741 gacccatttg ctcgaacgcc atcgcaacgc ccgttttatg gcgcatatgg acaactttct
     4801 cccaaactgg caaagcatca aacaacagct taatgccttg gagttatttg cacaaatata
     4861 taatttaaca taatatacat tatgcgaact atcggaaaca gttgtacgtg tccctgtggt
     4921 ctttccaagt aggaaaatta aagtatggcc aatgcggctg aatgtatagc ccggagcatc
     4981 cgcgtatccg aaggtactga acgacatgcc ctgcatccct ttcgacgctt ccatccagcc
     5041 ggtattggga aatacgttga ccttcaatat aatatacagg cagccgacac acaggatatg
     5101 ccgctgcttt ttcatcattt cttctgtcaa atccttggaa cggtcgattt gaaaacacgg
     5161 ctttacatac gccccagttc cctttgcagg gcttgaatat tttgctgcct gtccaataca
     5221 ttgctttgta atgcatttat ttcttgatgg ttgatgttgc cgccctttgc cagacgtgct
     5281 tgtgataaca ttttttgggc ttcagtcagg gctttgcgtt cgttgctcaa ttctgcttcg
     5341 agaatggagc gtctgctgtt gtttgagggt gcttgttgcg gcggcggcgt attggatttt
     5401 gccggcttgg atactgtttt gaccggggct ttatatttga caacctgtcc gccgtttgac
     5461 ggtgatgata ccggttcggg cgtttggggc gggatatagc gttcgctgct gtagttgccg
     5521 attgggggca aatcggttga gtggcagctt ttggacggct tggtggtgta aacggtttct
     5581 ccgttgattg tgcaggtgta gattttggcc gcattcgcac ccaatgggct tgaaatcagg
     5641 gaaaagttga ttaggattaa ggggagtttt gatttcataa tgtgcttgat gctcggataa
     5701 ttatttgatt ttttggtatt ttttataatg ctttcttggc gttttacggg cttttctttt
     5761 cggttgtttg cttttgattt tctttcgggg ctttggcagc ctgcttgttc tttttcccgg
     5821 caggtttttt gtttttcttt ttactttcta tgtgtatttt ggcttcttaa ctgagttttt
     5881 taattttcag gcggtatccg cctccctgat ggctgctgat tttaggtaaa tccgcatcgg
     5941 cgcacgatcc tgctggggct gatgtatata cgcttttgcc atttgagttg caatggtata
     6001 cggaggcttg cgccgccgcg ccggaaaggg acaggagacc caaggcggca aaaagtctga
     6061 tgtttatacc ggtaaaaggc ggtaataacg cccgaattat accgttattg gcaggcaaag
     6121 ataagcaccc tgcccgcgct tctttatcgc tcggcaaact gtttctcgat ttcagtttca
     6181 atccgttcca attcttcttg acaagccagc caaacctctt cgatttgggc aagttgtgtt
     6241 ttgacttttg ccagctggga taaggtattt tgcaattttt ctttgtttgc ttcaaggtaa
     6301 gcgtcttctt gtgctaaaaa tgcttcacat gccgtctgaa tttcggaaag ctgcgccatt
     6361 tctttttcgg cacggtctat tttctgctgt atcggcttgc cgcgtcgggc tttttcctga
     6421 cggatttgcg cttcgatgcg cttggtgtct ttgcggcttt ggctttgtgc ggatgctgcg
     6481 ggtgcggcgg cggcgttttc ctgtgccaaa cgccattggc ggtaatcgtt caaatcgccg
     6541 tcgaagttct tcagacggcc tttgtcgatc aggaggaagc tgtcggtagt cgcttcaagc
     6601 aggctgcggt cgtgcgatac gatgattaag gcgccttgga aactttgcaa cgcgagcgtt
     6661 aaggcgtggc gcatatccaa gtccaaatgg ttggtcggtt cgtcaagcag caacaggttc
     6721 ggcttttgcc agataatcat ggcaagggca aggcgggctt tttctccgcc ggaaaaaggt
     6781 tcggttttct gtaacgccat atcgccaaca aaattgaagc cgccgaggaa atttcggatt
     6841 tcttgttcgc gtacttcggg agaaagctgc tgaatatgcc aaacagggtt ttggtcggcg
     6901 cggatggtat cgagttggtg ttgggcaaaa tagccgatat tgagtttttc ggaacggacg
     6961 atgctgccag agagcaaatc gattttgccc gccaaggctt tgataaatgt agatttaccg
     7021 ctgccgttga cacccaataa accatagcgc gcgccgcttt ccagcgacag ggtaatgtcg
     7081 tgcaaaacag ttttgccttc gtaacccaaa tctgcgtgtt ccagcttcag caaaggattg
     7141 ggcagatgct cgggattgta aaactcaaag gaaaactcgc tgtccaaatg cgcgggagcg
     7201 atgcgttcga gctttgccaa agccttcatg cggctttgcg cctgaacggc tttggtggct
     7261 ttggctttga agcggtcgat aaaggattgc agatgtttga tttgcgcctg ctgtttgaca
     7321 taggcggctt gttgctgcgc gagacgctgc gcgcgttcgt tttggtaaaa atcgtaattg
     7381 ccgccgtatt gcgtgagctt ttgctgggac aattcgatgg tttgggtggt tgccgcattg
     7441 aggaaatcgc ggtcgtggga aatgatgatt tgcgtgcagg gtaaagaggc aaggtggttt
     7501 tccagccaca agacggtttc caaatccaag tggttggtcg gttcgtccaa aagcagcaaa
     7561 tcggcgcggc aaatcagggc ttgcgcaaga ttcaagcgca tacgccagcc gccggaaaag
     7621 gatttgacgg ggcggctgtg ttcttcttgc gaaaaaccca gcccgttcaa caattttgcc
     7681 gcgcgggccg gcgcggtata agcgtcgatt tcttccaatt tggcatgata ttccgcctgt
     7741 ttcataccgt cgttttgcgc ttccgcctgc ctcaatgccg tctgaagggt ctgtaactcg
     7801 gcatcgccct gcaaaacgta atctaaagcg gaaatatcca aatcgggcgt ttcttgggaa
     7861 acggaagcga gccgccagtt tttcggaatc gagacatcgc cgccgtcctg agtgatttca
     7921 cccttgatta aggcaaacag gctcgatttg cccgtcccgt ttttgccgat caaaccgacg
     7981 cgctgaccgg gattgacggt agcgttggct ttgtcgagca ggactttcaa accgcgttgc
     8041 agggtgaggt ttttgatttc aatcataacg gaaacatcgt cgggcgggaa aagcccgtat
     8101 tttacctgaa agtcagtgcc gatgccgtct gaaacgggaa atttacggct gaagccaagc
     8161 ccaagccctg cgcccttccg agtgcaggaa aaccaatgtc ctgaatgccg aatcggtatt
     8221 catacattcc acgccgattc cgattcgaga aaaatccgcc atgattttgg gatggataaa
     8281 ctcctgagcc gcgcccgtcc cgataatcaa tatttccaga tagtcaacag gtttgatgtc
     8341 ggacaacagg ttttccggag tcagatcgga caaggttcgg cattgcgaca ggcagaccga
     8401 atccttatgt acaagcacgg gtttatggaa actttgcccc gccagccgga ttccgcccgc
     8461 accgcattca tattccgcaa actgtccgtc tatcggattt tcttcaaaca acattttttt
     8521 accccgttgc cgcatcatct acaccgaaag gggtgcaaaa tcaggcgaat tcatgtagga
     8581 ttggcagatt tcatctgacc cgcctgccga tttcagacgg catttgattc aaagtgcggc
     8641 acaattatat cggcagcgga tattttcgtc tttcaatatt tacatttcag tcggcttaca
     8701 aggagacaca atgaagccag taaacatcgg tcttttaggt ttgggtacgg tcggcggcgg
     8761 tacggctgcc gtgttgcggg acaacgcgga ggaaatttcc cgccgcttgg ggcgcgaagt
     8821 gcgcatcagt gccgtatgcg acttgagcga agaaaaagcc cgccaaatct gcccgtccgc
     8881 agcctttgtc aaagatccgt tcgaactggt cgcgcgtaaa gacgtcgatg tcgtcgtcga
     8941 attgttcggc ggcacgggca ttgccaaaga tgcggtgttg aaagccattg aaaacggcaa
     9001 acacatcgtt accgccaaca aaaaactgct cgccgaatac ggcaacgaaa tcttcccgct
     9061 ggcggaagaa aaaaatgtca tggtgcaatt tgaagcggca gtagcgggcg gtatcccaat
     9121 catcaaagcc ctgcgcgaag gtttggcggc aaacaggatt aaatccatcg ccggcattat
     9181 taacggcacc agcaacttca tcctctccga aatgcgcgaa aaaggcagcg cgtttgccga
     9241 tgtactgaaa gaagcgcagg cattgggtta tgccgaagcc gatccgacct tcgacatcga
     9301 aggcaacgat gcgggccata aaatcaccat catgagcgca ctggcattcg gcacgccgat
     9361 gaacttttcc gcctgctacc tcgaaggcat cagcaaactc gacagccgcg acatcaaata
     9421 cgccgaagaa cttggctatc gcatcaaact gttgggcatt acccgcaaaa ccggcaaagg
     9481 catcgagctg cgcgtccacc ctaccctgat tcccgaaagc cgcctcttgg caaacgtcaa
     9541 cggcgtgatg aacgccgtgc gcgtcaacgc cgatatggtt ggcgaaacct tatattacgg
     9601 cgcgggcgcg ggcgcattgc cgaccgcttc cgccgtggtt gccgatatca tcgacatcgc
     9661 ccgcctggtt gaagccgata ccgcccaccg cgtaccgcat ctggcgttcc aacccgcgca
     9721 agtccaagcg caaaccatcc tgcctatgga cgaaattacc agcagctact acctgcgcgt
     9781 ccaagccaaa gacgaaccgg gcacgctggg gcaaatcgcc gcgctgttgg cacaagaaaa
     9841 cgtgtccatc gaagcactga ttcaaaaagg cgtgattgat cagaccactg ccgaaatcgt
     9901 gattctgacc cacagcacgg tcgaaaaaca catcaagtcg gcaatcgcag ccatcgaagc
     9961 actggattgc gtggaaaaac cgattaccat gatccgcatg gaaagcctgc atgactgagc
    10021 cgaaacacga aatgccgacg gaagagcagg ttgccgcgcg caaaaaagca aaagccaaaa
    10081 tccgcaccat ccgcatttgg gcatgggtca ttttggcgtt gctcgcttca accgccctgc
    10141 tctcccaatg cgcgatgtcc aaaccgcagg caaaacagaa aattgtcgag tcttgcgtga
    10201 agaatattcc gtttgccgaa aaatggcaaa acgatttgcg ggcccgcggt ttagattcaa
    10261 acaatacccg ccttaccgtc gactactgca aatgtatgtg ggagcagcct ttggacagat
    10321 tgagcgagaa acagattagt tccttcggca aactcggcgc acaagaacag cttgacctgc
    10381 tcggcggcgc aaatgccttt gaaacgcgag acaagcagtg tgttgccgat ttgaaatcag
    10441 aataatgtgg accgataaaa aagccgattc tttaaagaat cggctttttt cataaaaaac
    10501 ggcttacagt gcgtctttca atgctttacc tgcacggaat ttaggcgttt tggcggcggc
    10561 aatggtcaga ggctcgccgg ttttggggtt gcggccttgg cgttccgcac gttcgcccac
    10621 gtagaaagta ccgaaaccga ccaaagtaac ggtgtcgcct tgtttcaggg cggtggttac
    10681 tgcattggta gtggcatcca aagctttttg tgcggcggct ttggaaatgt cggcttcttg
    10741 agcaatcgct tcgatcaatt cagacttgtt cacaatcagt cccttcctgt cttaaaaaat
    10801 gatgaaatgc ccgaatactc ggggtttgta ctgcttgagc aactttcgct ttatagcaat
    10861 tctgaaattg ccgtgtcaag caaaaaatac ggaatcaccc tatttgacag gctttcagga
    10921 cgaaaccgca tttttacaac acatttcctg cgtttcaatg tttggttgcc ctgctgcggg
    10981 gttttggttt tgaagcggat tccgccaccg cttccgcacc agaaggttct gcccaaggct
    11041 caggctggct ttccaaaccc agagccaata cctcgtctat ccatttgacc ggatggatgg
    11101 tcaggccggt tttcacgttt tcagggattt cttccaagtc tttgaagttg tcttttggaa
    11161 ttaaaacgtg tttgatgccg ccgcgaagcg cagccaacag tttttccttc agaccgccaa
    11221 tcggcaggac ttcgccgcgc agggtaattt cgcccgtcat cgctacatcg gcgcgtaccg
    11281 ggattttgga aaatgcagac accatcgcca aagtcatggc aatgcccgca ctcggaccgt
    11341 ccttcggcgt tgcaccttcg ggaacatgga catggatgtc ttttttctcg taaaaatcag
    11401 aagccaagcc cactgattcc gcgcgggagc ggacaaccga ccacgctgca gacacggatt
    11461 ccttcatcac atcgcccaac tggccggtgc actgaatcac gcccttaccc ggcaatgctg
    11521 cggcttcgac ggtcagcaat tcgccgccga cttctgtcca cgccagaccg gttacttgtc
    11581 cgatacggtt ttcgctttcg gcaacgccgt aatcgaagcg gcgcacaccc aaatagtcgt
    11641 gcagattttt ctcatttact ttaaccgctt taggtttggc tttgctggtt ttcttggttt
    11701 cagacaacct cttcttatct tcgtccaagg taatctgcat caccaccttg cggcagattt
    11761 tggcaatctc gcggtcgagc gaacgcacgc ccgcctcgcg ggtgtagtaa cggataatat
    11821 cgcgcaccgc gctttcttcg acgagcaatt cgccttcttt cacgccgttg cgcttcattt
    11881 gcttcggcac gaggtactgc atcgcgatat tgattttttc gtcttcggtg tagccggaca
    11941 gacggatgat ttccatacgg tcgagcagcg gggtcgggat attcaggctg tttgaagtcg
    12001 caataaacat cacatcgctc aaatcgtaat ccacttcggc ataatgatcg gcgaacttgt
    12061 tgttttgctc gggatcaagc acttcgagca acgcgctggc gggatcgccc cggaagtcgc
    12121 tacccaattt gtcgatttcg tcgagcagga acaatgggtt ttttacgccg gcttttgcca
    12181 tattctgcaa aatcttaccg ggcatggagc cgatataggt gcggcggtgt ccccggattt
    12241 cgctttcgtc gcgcacgccg cccaaagcca tgcggacata tttccgcccc gttgctttgg
    12301 cgatggattc gcccaaagag gttttgccca cgcctggagg gccgaccagg cacagaatcg
    12361 ggcctttgag tttgtccata cgtttttgga cggcgaggta ttccaaaatc cgttctttga
    12421 ctttttccag gccgtagtgg tcggtatcca gcaccaatcc ggctttggcg atgtctttgc
    12481 tgacgcggga ttttttcttc cacggcagtt cgagcagggt gtcgatgtag ttgcgtacga
    12541 cggtggattc ggcagacatc ggcggcatca ttttgagctt tttcagttcg gacaggcatt
    12601 tttcttccgc ttctttgctc atccccgcct ttttgatgcc tgcttccaag gcatccagtt
    12661 cgccgttttc gtcttcttcg cccaattctt tgtgtatcgc tttaatctgt tcgttcagat
    12721 aatattcgcg ctgggatttt tccatttggc gtttgacgcg tccgcgtatg cgtttttcgg
    12781 cctgcataat gtcgagttcg gattccagct gtgccagcag gaattccatc cgtttgccga
    12841 tttcgggaat ttccaaaatc tgttggcgtt gcgccagttt caactgcaaa tgcgctgcga
    12901 ccgtatcggt tagccggctg ttttcggcaa tgccgttgat gctgccgata atttcggcgg
    12961 ggattttttt attgagtttg gcgtattgtt caaactgcgc caacagggtg cggcgcacgg
    13021 cttcgaggtc ggtattgccg cccgtgtctt cttccacgac cgcctctata tgggaaacga
    13081 acagaccgcc cgtgtcttcg atggtcagaa cacgtccgcg atacagacct tcgaccaata
    13141 cttttaccgt gccgtccggc agtttcaata cctgtaagac ttgtgcgacg gtaccggtct
    13201 gatacaggtc ggcggcaacc ggttcttcta gcgccgcatc ggtttgtgcc aataggaaaa
    13261 ccggttcctc gcgggtaatg gcgttttcca gtgcggcgat ggatttcggc ctgccgacaa
    13321 acagcggcag aaccatatgc gggtaaacga cgacatcccg caaaggaagg gttgccaagg
    13381 cggcatattc ctcaaaatgc ttttcttttt gtgtcatagg tactctcttg tgtctgacag
    13441 attgccgatt ttcgcgtaca ttggggttga aggtattatt tcaagcatat gtggtttatt
    13501 tatggagttt gatgcgatgc cgtctgaaac attccggctt cagactgcat gggcttggaa
    13561 agacaaggcg ggaacaaaaa actgttctgt gttgccgctc cttgctgtac catccgtatg
    13621 gtttacggtt ctgccgccct attttcaaaa catgacgcag gtatcccatg tcttctttta
    13681 tttttcccga taacggggcc gaatggcgca acgaaagtgt gcaaaagccg cccaaaggtt
    13741 ggcattatgt ccgcgaaagc ggtgcagacg gcattttgaa ggctgcctat caaaatattg
    13801 caactgtctg gcagggcgat ttccacaatg ccaaacaggt gctttctgca atgaagaaga
    13861 gggctcgcaa acctgccgcc gtccgttccg atgcggatat cgccgccctt ttccatgccc
    13921 accgtatgaa gcaggcgcag cagagccgta ttctgaatat gcttgccgtt gaaatccgcc
    13981 ccggttttgt gttggacaac aaacgcgcgc ccgatatacg cgccgccctg ctcgacgtgt
    14041 acggagaggc ggacggcaaa ccgtttttcc tgccgctcaa tctgctgctg gggtttatgg
    14101 gtgcgcacga gtggcataag aaaggggttg ccgttccgca gctgggcggc agcatacacg
    14161 ttcctttcgg cgtattctcg ccgttgcgcg gcgaatacct cgacctgctc gcccatgcgc
    14221 cgtcaacggg ttttcagacg gcattcgata tcgggacggg ctcgggcgtg cttgccgcca
    14281 ttttggcgaa acagggcatt ccttccgtca tcggcacgga taccaatccg aaagccgtcg
    14341 cctgcgcccg tgccaatatt gcccgtttgg gctttgaaaa acaggttgag atacgggaaa
    14401 ccgatctgtt tcccgaaggg tttgccgatc tgattgtctg caatccaccc tggcttcccg
    14461 ccaagccgac ttccgccgtc gaatccgcgt tatacgaccc cgaatctgcg atgctggctg
    14521 cgtttttgcg ggatgcgccg aaacatctga atcccgacgg agaaatccgc ctgattattt
    14581 ccgaccttgc agagcatttg gggctgcgcc ctgccgattt tttggaaaag gcatttatcc
    14641 gggcgggttt gcgtgttgcc gatatgatga aaaccaagcc gaagcacaaa aaagccgcga
    14701 atccgagcga tccgcttgct tttgcgcgaa cccgggaaac cactttccta taccgtttga
    14761 aaaaggcata aggggcggcg gcgcggcatt cgggcggatt attcttgtga aaatacccgc
    14821 tcgagcatac tgcccaatgc cgtctgacgc gttttgacag tggcggcagc ttgccggaag
    14881 gtttcttctc cgccgaagaa tacgaacttc tccctcgcgc gggtaatggc ggtatataac
    14941 agctccttac tcaatccgga caatgcatcg tccccttcgt ccgaaggtgc ggcggaaggc
    15001 ggcagcagcc agacttcccg atattccgaa ccttggcttt tgtggacggt catggcgaat
    15061 gcgggttcga aatcgggcag gcagcttacc gctacctttt taaatccgtc cgcatcggca
    15121 aaataggcgg caaggctgcc ctgccgtccg acatcttcca taatcaatcc gatgtcgccg
    15181 ttgaacagtt caagcgcgta gtcgttctgt ctgatcataa tcggttctcc ggcgaaatag
    15241 gcctgatgtt ccggtatgtt cattttgcgg cgtacatggc tgcaataggc ttcgttgaag
    15301 tcttccgcat cctgccgcca agctgccagg accacgatat ccgagatgcc tgcgtatgcg
    15361 gcttcgatat tgccgtcttt taccgcctgc caataggctt tgtgcgcccg gtacaacctt
    15421 tcgactcgag cgttcggact gcattccgaa tgttccagtt cgtccggaaa ccggtcaaac
    15481 aatgcccacg ccccctcatc gcccgatacg gcggcacggg caaggcagcc gatgccactg
    15541 ttgtcgccga agcggtggct gaacgacaga tgggcggtgt tttgcgccaa cacgggcgga
    15601 tttgcgctga cgctgaaacc gtgttccgga aggaagccgg ccagcctttg gtgcgtttct
    15661 ccgtccaaaa cggttttgtg cgacaaaacg gacagcaccg ccccgattcc gacggacggg
    15721 agctggtttt catcccccag cagaatcacg cgcgcgccgg ttttgaccgc ttttaaaagt
    15781 tgcaacatca atgcggtatc caacatggag gcttcatcga taaccaatac gtccaacggc
    15841 agcgggtaaa cagggttgaa cgccgcctgc attttgggcg ggcgcagctt cagcagtcgg
    15901 tggacggttt gcccttccag tttgagcaaa tggcggcgga tggcctccgg cgcgtcaaaa
    15961 ccgttgattg cacggtgcag cgcgcgcgcc atatgtgccg ccgctttgcc tgtcggtgcg
    16021 gcaagcgcga tgttgggaag attttcgtct tcaccgcaaa tcagcgccag cagtttggca
    16081 accgttgtcg ttttgcccgt tcctggcccg ccggtaatca ccataaaaga ctgcaacagt
    16141 gccaaggcgg cggcatcgcg ctgcccttcg ctgcccgtgc cttgaaacca ttttgcgagg
    16201 ttttgccgcg cgcctgccgc gtcgggggcg gacgtgccgg ctgccgccaa gcgttttatc
    16261 tcggcagcca aatcgtattc caactgccac atcctgccca aaaacagcct tctgccttcc
    16321 aaaatcaaag gcgcggcgga tgttccgacc acgggtgcga gtgccgacag cgcgtcagcc
    16381 tcgccaccgc tcaaacggat aaacgaatga ccgttttgca atgcctgaaa caggcgttcg
    16441 gtgcagtttg caagcacttc gctgcctgaa cccgcatagc gtgccaaaaa ccagattgcc
    16501 gcccttgccg ccgcttgggc aaattcatct gtctgccgtt ccattgtatt ccttatccaa
    16561 atgccgtctg aaggcgtggg gcttcagacg gcgcggtgtt gtttcggctg tttaggcgtt
    16621 tgcgccctgt ttggggtgca ggctgccgtc tttcatgacc atcacgcgct cgaagcggcc
    16681 ggcgagttcg tcgtcgtgcg ttacgaccac cagccccgtt cccaattctg ttttcagttc
    16741 cagcatcata tccaaaacat tcctggcgtt cgcacgatcg agattgccgg tcggttcgtc
    16801 ggcaagcagg catttgggtt gcgtaaccaa ggcgcgtgca atggcggcac gctgccgctc
    16861 accgccggac agttcgcccg cgcggtgcgc cgaacggtgc ttcaatccga ccttttccag
    16921 catcgccatt gccgtttctg ccgcctcttc ccgttttttc ttgccgatca gaagcggcat
    16981 catcacgttt tccagtgccg aaaattcagg cagaagatgg tggaactggt acacgaaacc
    17041 gagatggcgg ttgcgcagga tgcccagttg tcgctggctt aatgtacgca aatcctcgcc
    17101 catcagcagc accctgcctt cagacggcat atccagcccg cccaaaatat gcagcagcgt
    17161 cgatttgccg ctgcccgaag aaccgatgat gccggtactt tcccccgcga ggatttccaa
    17221 atccaagccg tgcagcaccc gaacgtccaa accgccgtca cggtagcgtt tgcccacgcc
    17281 ttcgcatttc aaaatcaact cactcataac gcaaagcctc cgccggttgg gtttttgacg
    17341 cgcgccagct cgggtagagc gtcgccacga aagacagtcc caaagaaatg caggcaatca
    17401 gggcaacgtc gcccatatcg acatcgctgg gcaggtagtc gataaaataa acctgcgaat
    17461 tgataaggtg gacaccgagc aggttttcaa aaaacgccac gactctgccg acgttccaac
    17521 ccaaaagcac gccgcagacc acacccgcca gcgtgccgaa aaagcctgaa aacgcgccct
    17581 gcaccataaa aatcttcatc acgccggcag gggaaagacc caaagtccgt aaaatcgcaa
    17641 tgtccgcctg cttttccgta accgccatca ccaaggaaga gacaaggttg aacgccgcca
    17701 cggcgataat cagcgtcagg atgatgaaca tcatccgttt ttccaactcg accgcctcaa
    17761 aatagcttcg attgctgaac gtccaatcgc gcacccaaac cgtatccctt tgcgcctccg
    17821 gaatcagctt tgccgtcaag gcgggggcgt tttgcgggtc ggcgagtttc agccgcagcc
    17881 ccgcaacttc cttatccaag cggtacagta cgcgcgcgtc ttggatatgc gtcatcgcca
    17941 aggagttgtc cacttcgtaa acacccgtct taaccagacc gaccacggta aactgtttca
    18001 acctcggtac gactccggcg ggcgtaacat tgccctccgg cgtgatgacg gtaactttat
    18061 tgccgacttc cgcccccaaa gcctccgcca agccgacacc gaggataatg tcaaactcgc
    18121 ccggaatcag gtcttcaaat ttgcctgccg gcattttgtc gccgtattcc accactttgc
    18181 gctcttcaga cggcaaaatg ccgcgcatct gcacgcccct gatttcgccc gcattggcca
    18241 gcaatgcctg attggaaaca tagggcgcgg cagccaaaat acctttgcgg ttttcggtaa
    18301 atcgaagcag gttgcgccaa tccgtatccg tattgtcgat atagccgatt tcggcgtgcg
    18361 gcgcgacatt caggagctgc ccgcgtattt ctttctgaaa gccgttcata accgacaaga
    18421 cgacaatcag cgcggttacg cccaaggcga ttccggcaat cgaaaccatc gtgataaacg
    18481 acataaagcc gttgcgcttt ttcgccctga gatacctcaa gcctatccaa gcctctagag
    18541 aaaacataac gctaccttaa aaatgtctgc aaacgtgccg ccccggacgg cggtttgggg
    18601 gcggcggcaa aagtttattg taccgtaaaa cccaggcagc gtccgaacgc ccgagtgcgg
    18661 gggacggggc ggcagagcgg gcggaaaact tgcacaacgc gtcaaactgc cctatccttt
    18721 ccttcagaaa aaccgtttct tgaggaaaac aatgaatatc cgaactgctt ttgctttgtg
    18781 cgccatcgcc ttatccgccg ctgcggctgc ctacgccaaa gaaatcaaaa tcgatgccaa
    18841 caacacgcct tattccgaag ccgacgcgca aaagctggcg gcaacggcag tcggtatggg
    18901 tgttaaggaa cctatcagcc tgaacggcgg cagcggcagc attaccgtgt ccggcagcag
    18961 cgcgacgcag tgcgtgttca aagtcggcaa cggaggcgca ttgcagattc aagggctaaa
    19021 ctgcaagtaa accgcccgga aaaatgccgt ctgaaggctt cagacggcat tttgcattgg
    19081 cggcgttatg ccccgccttc tttaatcagg cggcgttcgt acaccgcctg cgccagcgtt
    19141 cccgcatcga catattccaa ttcgccgccc aaggggatgc cctgcgacag cctgctgact
    19201 ttgtaaggca ggtttttaaa aaactcggac aggacatacg ccgtcgcatt gccttctgcg
    19261 gtaaaagcgg ttgcaataat gatttcttcg acttccccgc cgcccagccg ttgcgccagc
    19321 ctgtccaatg cgatggcgga tacgtccatt cccaatgccg tattgatttg ccccatcagg
    19381 acgaaataca gcccgtcgtg gcagtttgcc acttccatat tcgacacgtc ggcaggcata
    19441 tgcaccacca tcagccgccg cccgtcgcgt gtttcatcgg cacaaatatc gcacaatccg
    19501 ccttcgcaaa acgtgttgca catcgcgcaa tggtgaacct gcttcaatgc cgtctgcaag
    19561 gcatccacca gtttttcagc ctctttgcgc ttgtgttgga gcaaatgata cgctatccgc
    19621 tgtgccgatt tcggcccgac gttgggtaaa accttcagcg cgtcgatcaa tccttggaag
    19681 gcatcttgtt ttttgtggct catcatattc cgccgtatgg gaaaacggcc ggaatattcc
    19741 gaccgttatt ttgtcaacaa aagtgtcaat tactgaccgc cgccgttgtc gaccgattgc
    19801 gctcctttgg tctgtttgat tttgccgttg aaataacgta tcaacaagtc gaaggtattg
    19861 gcagactgct gctgcgccaa agcctgtttt gcaagcggaa gctgtgcggc gatatcatcc
    19921 ggcggggtta tagcctgtac ttcgacaatc acgggtgccg gcagaccggt cagcctgacg
    19981 taggcgggtt tgccgtttgt cggttttgct ttcagcagtt ccgcataagc ctcgggcggc
    20041 atggactgcc ttgcctgctg cgcgcccaaa acggacactt ccgaccattt cacatcaact
    20101 gccttgccgc cgttcagttg ggtaagcacg tcttttgcct tgttttcggc aagtttggcg
    20161 gcttcggcac ggatataagc ctgacgcacc gcatctttgg cttcggcaaa cggcagggtt
    20221 ttctcttcgc ggacttcttt ggcgcggacg acccacgcga tttcattatt gatagacaac
    20281 acttcggaat tgtgtttttt cttcaatacg tcgtcgctga atatggcatt gatcaggttt
    20341 tcaggcagtc cggacatttg cgcgtcctgc ctgctcagcc aagtttcttg ggtttcgact
    20401 ttcaaactgc tgtttttggc ggcttcggca agcgaagcgg gatggttgaa cgcgtcatcg
    20461 ctcagctttt cttttgcctt attgaagtcg gcaaccgcct ttttcatttt caattcgttt
    20521 tcgacggcgg ctttttcctg ctcgaaagaa agtttggctt catttgccgg caaacgcgcc
    20581 acgcgctctt caaacgcatt tttcacttcc gtttcactga cggtttgctt gtctgcaaaa
    20641 tccttcagat tcaaggcgac atattccaat ttgaccgcct gcggcagcag atagtctttt
    20701 ttgttcgcat tataaaattt ctgcaaatcg gcttcagaca ctttgacttg ggcgatgaac
    20761 tcgtcggggt tgaaagtgtg cgaacggatg gtgcggttga cctgcgtcag cctgatcagc
    20821 tgttccgcct gcgcgtcgcc gaccaatacg ccgttttgga cgaggtttac caaattctgc
    20881 aaggcaaact gatcgcggat ttcttcgaca aactggtctt cagacatatg gcgttgggaa
    20941 aggtagcggt ttaaaagcgc gtggtcgaat ttgccgtttg cgtcgtggaa attgggatcg
    21001 tccacgataa tttgcttgat ttgttcggaa gaaaccgaaa tgcctaacag cttcgcgccc
    21061 tgtttcaggt aggcgcgttg cagcagggat tggaacaccg cgtcgcgcga agggccgccg
    21121 ccgtccgcct gttcgttctg tatggcgttg ttgatggagt ggtcgctgat tttttcgtcg
    21181 cccacttgga cgatgtagtc ggcacccgga tgggataccg tgctgacccc gaagccgacg
    21241 aaggttaatg caatcaggcc caaaaggact tgggcgggcg ttctgtattt ttcgatggaa
    21301 tggaacatat tttaaatcgg gatatagaat gggaaattca agtcgggtat tgtaacggtt
    21361 tttatccctg tctgcacggg gcttgccggt tgaagatgcc gtcgtaggtt tcttcgctga
    21421 agccggcgta aaccttgccg ccgcactcca atacgggacg cttgatcagg ctcggcattt
    21481 cggacatcag tttgacggcc tccgctgtcg aggacagggc tttttgctgt gtttcggcat
    21541 cgagtttgcg ccagcttgtc ccgcgtttgt tgagcagggt ttccaaaggc acttgttcca
    21601 gccacgagca gatttccgct tcagacggca tctgtttttt gaaatcccga aactcaaact
    21661 ccaagccgta tccggcaagt cggtttttgg cttttttgac cgtatcgcaa tttgggatgc
    21721 cgtgaaggac tatcatttgg aaaccttttg tctgaaataa taaaacggat attttactat
    21781 aagtgtctga aaatttgccc gtctgtttca gacggcgggg cggttatgtt acaatccgaa
    21841 aattcgaaaa atttaatctc ttgttcaata aaggctttac caatcatgat ttctaccaac
    21901 ggcatcacca tgcagttcgg cgcgaagccg ctatttgaaa acgtatccgt caaattcggc
    21961 gaaggcagcc gctacggttt gatcggcgcc aacggctcag gcaaatccac cttcatgaaa
    22021 atcctcggcg gcgatttgga acagaccgcc ggtgaagtcg caattgaaaa cggcgtgcgt
    22081 ttgggtaaat tgcgccaaga ccagtttgcc tacgaagaca tgcgcgtgct ggacgtggtg
    22141 atgatggggc ataccgaaat gtgggcggcg atgaccgaac gtgatgcgat ttacgccaat
    22201 cccgaagcca ccgaagacga ctacatgaaa gccgccgaac tggaagccaa gttcgccgaa
    22261 tacgacggct acaccgccga agcgcgtgcc gccgaactgt tgagcggcgt gggcatttcc
    22321 gaagatttgc acaatgcgaa aatggcggaa gtcgccccgg gcttcaaact gcgcgtattg
    22381 ctggcgcaag ccctgttctc caagccggat gtgctgttgc ttgacgaacc gaccaataac
    22441 ttggacatca acaccatccg ctggctggaa ggcgtgttga accaatacga ctccaccatg
    22501 atcatcatct cgcacgaccg ccactttttg aacgaagtct gcacccacat ggcggatttg
    22561 gactacaaca ccatcaccat ctatcctggc aactacgacg actacatgct cgcttccgcc
    22621 caatcgcgtg agcgcgccct gaaagacaac gccaaagcca aagagaaact gcaagagctg
    22681 caagagttcg tcgcacgctt ctctgccaac aaatccaaag cccgtcaggc aaccagccgt
    22741 ctgaaacagg ccgacaaaat caaatcggag atggtcgaag tcaaaccttc cacccgtcaa
    22801 aacccgtata tccgttttga agccgatgaa aaagccaagc tgcaccgtca ggctgtggaa
    22861 gttgaaaaac tggcgaaacg ctttgaaacc cagttgttta aaaacctgaa cttcatcctt
    22921 gaagcgggac aacgcctcgc catcatcggc ccgaacggcg cgggcaaatc caccctgctg
    22981 aaactcttgg ccggcgcgta caaccccgaa tattcagacg gcctgttacc ggacgaaggc
    23041 agcatcaaat gggcggaaaa agccagtgtc ggctactatc cgcaagacca tgaaaacgac
    23101 ttcgacgtcg atatggattt gagcgaatgg atgcgccaat gggggcagga aggcgacgac
    23161 gaacaagtca tccgcggcac tttggggcgt ttgctcttcg gcagtaacga tgtcgtgaaa
    23221 aaagtgaagg ttctctccgg tggtgaaaaa ggccgtatgc tttacggcaa actgttgctg
    23281 ttgaaaccca atgtcttagt catggatgaa ccgaccaacc acatggacat ggaaagcatc
    23341 gaatccttga acatggcgct ggaaaaatac aacggcacgc tgattttcgt ctcgcatgac
    23401 cgtcagtttg tatcttcatt ggctacccaa atcatcgaat tggacggtaa aggcggatat
    23461 gaacactact tgggcgatta cgaaagttac ttggagaaaa aaggcgtagc ataagcgcac
    23521 aaaataccct ccggttgaaa caatgccgtc tgaagccgct tcagacggca ttgttgataa
    23581 ctttaaaata ggaagcatat gcagacttac ctcgtcggcg gcgccgtccg cgattatctt
    23641 ttgggcttgc ccgtcaaaga ccgcgattgg gtggtcgtcg gcgcagacgc acaaaccatg
    23701 ctggcgcaag gcttccagcc agtcggcaaa gactttcccg tgtttctcca tcccgaaaca
    23761 cacgaagaat acgccctcgc ccgcaccgag cgcaaaaccg ccaaaggtta cgccggcttc
    23821 agtttccaca ccgacaaaga cgttacgctg gagcaggatt tgatgcgccg cgacctgacc
    23881 atcaacgcga tggcgcaaga tgcggacggc aagattatcg accctttcgg cggacaacgg
    23941 gatttggcgg caggcatttt gcgccacgtt tccccggcct tcgccgaaga ccccgtccgc
    24001 atcctgcgta ctgcccgctt tgccgcgcgt tacaagtttg aaatcgccga agaaaccata
    24061 aagctgatgc ggcagatggt ggaaaacggc gaagcggacg cattggttgc cgaacgcgtc
    24121 tggcaggagt ttgcgaaagg tttgatggaa aaaaatccgc gcaaaatgat tgaagtgttg
    24181 cgcgaatgcg gcgcgctcaa agtcttgctg cccgaagtca atgccctctt cggcgtgccg
    24241 caacgcgccg actaccatcc cgaaatcgac agcggcatcc ataccctgat gacgctgcaa
    24301 cgcgccgccg atatgggttt gagcctgccc gaacgctatg ccgccctgct gcacgacttg
    24361 ggcaaagcca aaacaccgtc cgacatcctg ccgcgccacc acggacacga cctcgccggt
    24421 gtcgaacccg tgcgcgaagt caatcagcgg ctgcgtgcgc cgaaacattg cgccgagctt
    24481 gccgaacttg tctgccgttg gcacattatt ttccaccaag tcggacagct taaaagccaa
    24541 accattctga acgttttgaa aaaaaccgac gctttcagac gccccgaacg ctttcagacg
    24601 gcattgaacg tctgcattgc cgacacgcaa ggccgtctga accgcgaaca cacgccctac
    24661 ccgcaacgcg cgcactggct cgccttactc gaagccgcca atcaggcgga ttcgggcaaa
    24721 atcgccgccg aatgccgctc gcagggaaaa gcgcacctta tcgccgaaca aatcgaccgg
    24781 gcgcggctgg cacaaatcgc cccattgcaa aaagcgtttc gagcggcgca agacaaaaca
    24841 gaaaaacatt aaaacgtcca atgcagcca
//
LOCUS       DAXHMX010000011        24183 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHMX010000011 DAXHMX010000000
VERSION     DAXHMX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.011"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..280
                     /locus_tag="ACFM4S_000338"
     CDS             <1..280
                     /locus_tag="ACFM4S_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6050092.1"
                     /translation="AYSERWRQIKRHSQYLIGGKLRLWQKRFWEHTIRDEADFACHFD
                     YLHFNPVKHGYVGQISDWRFSTFHRYVKQGIYPHNWGGGNADFSIEYD"
     gene            352..513
                     /locus_tag="ACFM4S_000339"
     CDS             352..513
                     /locus_tag="ACFM4S_000339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050093.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            563..847
                     /locus_tag="ACFM4S_000340"
     CDS             563..847
                     /locus_tag="ACFM4S_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050094.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWARGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(972..1688)
                     /gene="trmB"
                     /locus_tag="ACFM4S_000341"
     CDS             complement(972..1688)
                     /gene="trmB"
                     /locus_tag="ACFM4S_000341"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6050095.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTSAQQRAIDTLWG
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1810..3213)
                     /locus_tag="ACFM4S_000342"
     CDS             complement(1810..3213)
                     /locus_tag="ACFM4S_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6050096.1"
                     /translation="MNNPDLLYHQALERLSQEQYDFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPHQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTTAKNNNPHGQYLLAQYCQYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYSLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPNQYGGIK
                     DSAIRRQRAERLYQKAQALHYRLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDNEKACRYLQEAINSGH
                     EQKSFLQELLQQWQNAV"
     gene            complement(3332..5218)
                     /gene="uvrC"
                     /locus_tag="ACFM4S_000343"
     CDS             complement(3332..5218)
                     /gene="uvrC"
                     /locus_tag="ACFM4S_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6050097.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCSAPCVGHISEEDYHDSVREASTFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLMRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVVAASKEDLEQVEGISKALAETIY
                     EHLH"
     gene            complement(5282..7444)
                     /locus_tag="ACFM4S_000344"
     CDS             complement(5282..7444)
                     /locus_tag="ACFM4S_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6050098.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLTIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMD
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDEDDLYRI
                     AAAVEQNATHPLARAIVSAAQTRSLEIPAAQNAQTVVGAGIAAEVEDVGLVKAGKAEF
                     AELTLPEFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            7583..8533
                     /gene="trxB"
                     /locus_tag="ACFM4S_000345"
     CDS             7583..8533
                     /gene="trxB"
                     /locus_tag="ACFM4S_000345"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6050099.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            8689..9057
                     /gene="rpsF"
                     /locus_tag="ACFM4S_000346"
     CDS             8689..9057
                     /gene="rpsF"
                     /locus_tag="ACFM4S_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6050100.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAAAQ"
     gene            9058..9360
                     /gene="priB"
                     /locus_tag="ACFM4S_000347"
     CDS             9058..9360
                     /gene="priB"
                     /locus_tag="ACFM4S_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6050101.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGISVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            9367..9597
                     /gene="rpsR"
                     /locus_tag="ACFM4S_000348"
     CDS             9367..9597
                     /gene="rpsR"
                     /locus_tag="ACFM4S_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6050102.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            9614..10066
                     /gene="rplI"
                     /locus_tag="ACFM4S_000349"
     CDS             9614..10066
                     /gene="rplI"
                     /locus_tag="ACFM4S_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6050103.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADAQARQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VATGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(10259..11065)
                     /locus_tag="ACFM4S_000350"
     CDS             complement(10259..11065)
                     /locus_tag="ACFM4S_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6050104.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(11066..11812)
                     /gene="pssA"
                     /locus_tag="ACFM4S_000351"
     CDS             complement(11066..11812)
                     /gene="pssA"
                     /locus_tag="ACFM4S_000351"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6050105.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(11886..12290)
                     /locus_tag="ACFM4S_000352"
     CDS             complement(11886..12290)
                     /locus_tag="ACFM4S_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050106.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            12396..12878
                     /locus_tag="ACFM4S_000353"
     CDS             12396..12878
                     /locus_tag="ACFM4S_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6050107.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGALR
                     VDNAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(12930..14141)
                     /locus_tag="ACFM4S_000354"
     CDS             complement(12930..14141)
                     /locus_tag="ACFM4S_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6050108.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSIGEWGLPSTMFGLFAAGFMYFVFAVLIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            14364..16802
                     /locus_tag="ACFM4S_000355"
     CDS             14364..16802
                     /locus_tag="ACFM4S_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6050109.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            17010..18320
                     /gene="tig"
                     /locus_tag="ACFM4S_000356"
     CDS             17010..18320
                     /gene="tig"
                     /locus_tag="ACFM4S_000356"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6050110.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAQ
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAMFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18416..19030
                     /gene="clpP"
                     /locus_tag="ACFM4S_000357"
     CDS             18416..19030
                     /gene="clpP"
                     /locus_tag="ACFM4S_000357"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6050111.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(19087..20352)
                     /gene="ispG"
                     /locus_tag="ACFM4S_000358"
                     /gene_synonym="gcpE"
     CDS             complement(19087..20352)
                     /gene="ispG"
                     /locus_tag="ACFM4S_000358"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6050112.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLAFSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(20368..21129)
                     /gene="pilW"
                     /locus_tag="ACFM4S_000359"
     CDS             complement(20368..21129)
                     /gene="pilW"
                     /locus_tag="ACFM4S_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6050113.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(21132..22226)
                     /gene="rlmN"
                     /locus_tag="ACFM4S_000360"
     CDS             complement(21132..22226)
                     /gene="rlmN"
                     /locus_tag="ACFM4S_000360"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6050114.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22370..22795)
                     /gene="ndk"
                     /locus_tag="ACFM4S_000361"
     CDS             complement(22370..22795)
                     /gene="ndk"
                     /locus_tag="ACFM4S_000361"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6050115.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(22854..24005)
                     /gene="zapE"
                     /locus_tag="ACFM4S_000362"
     CDS             complement(22854..24005)
                     /gene="zapE"
                     /locus_tag="ACFM4S_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6050116.1"
                     /translation="MSSRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5708 a         5859 c         6666 g         5950 t
ORIGIN      
        1 tgcttattcg gaacgctggc ggcagatcaa gcggcacagc caatatttaa tcggcggcaa
       61 actcaggctt tggcaaaaac gcttttggga acatactatc cgcgatgagg ccgattttgc
      121 ctgccatttt gattatctgc atttcaatcc ggtcaaacat ggctatgtag gacaaatttc
      181 cgattggcgg ttttctacgt ttcaccgtta tgtcaaacag ggtatttatc cgcataattg
      241 gggtggcggc aatgcggact tttctattga atacgattaa agtaatgtcg gattcaagaa
      301 tccgacctac ggaaaactaa agattttata cgggctacgg cttgctggct tatgcgttgg
      361 cttaatttga aaaacagcac atcggttcat aatacacaag gtattagaac aaaagaagca
      421 gttgaacagg gattgcgtgg taataataga cctgaaaaag gaataggtaa tagatggaat
      481 tatgatggat ttataaagga attagcgaag taacaggtat tctaaatatt tctgataaat
      541 ataaagaaca aggagtaaaa aaatgaaagt aatcactatt acattgttta ctagctgcat
      601 actatctgga tgctatgggc ttgaatatgg aatgagtggc ggcattcctc atgatgactg
      661 ggcaagaggt agccatgtta gtcaagccaa agcagatgct gcgtggaaag aatgttctga
      721 caaatatatg agaaatggtg gttcaggatt agtagaacaa tgtatgaatg ataaaggatt
      781 aatacgttta tctgaaatac gtcgtaggca aaagagagca gaaacacaaa gagcagtaat
      841 taagtaacaa taatgaaata ctactgttcc cgaacaaagc ggcatttacg caggtgatga
      901 cggctatatt ttgaaaaatg ccgtctgtaa gcaaacgtgc ttcagacggc atttttttat
      961 ccggcggtct gttatccgat ccgtttgaat accaagtccc aaacgccgtg tccgaggcgt
     1021 ttgccgcgcg cttcgaattt ggtttcgggg cggtagtccg gggtgggggc gtagtctgcc
     1081 gccgtatttt gcaggctgtc gaagctgctg aggacttcaa gcatctgctg cgcatattct
     1141 tcccagtctg tcgccaagtg gatatagccg ccggttttga gtttgggcag cagtttggcg
     1201 atgaaggggg cttgtatcag acggcgtttg tggtggcgtt ttttgtgcca cgggtcgggg
     1261 aagaatatgt ggatgccgtc gagcgagccg tcttgcagca tattttcgac aacttctacg
     1321 gcatcgtgcc gcatcacgcg gatgttttcc aaatggtttt cttctatgag tttgagcaga
     1381 ttgcctacgc cgggaccgtg tacgtcgatg gcaagaaagt cggtttcggg cagcctgcgg
     1441 gcgatttctg cggttgccgt ccccataccg aagcctattt cgaggatttt gggtcggctg
     1501 cttccgaaac gggcatcaag atcggccggt gttgcttggt agtcgatgcc gaagctgccc
     1561 cataaggtat cgatggcgcg ttgctgcgcg gaggtcatgt ggctttggcg gaggacgaaa
     1621 ctgcggatgc tgcgggggtg ttcggcttgc cagttgtttt gtgtttggtt ttcggcggtg
     1681 tctgtcataa tgctttcgtg cgcttgtgca atcaggatgc cgtctgaatg tgaaatgcct
     1741 gttcggacgg cgggaaagga aaggggcgat tatacctgtt tcgtttcggc agggtaggtg
     1801 taacgctgtt cagacggcat tttgccactg ttgcaacagt tcttgcagaa agcttttttg
     1861 ttcgtgtccg ctgtttatgg cttcttgcag gtagcggcag gctttttcgt tgtccggccc
     1921 gacaccgtgt ccgctgtaat ggatgcaggc gaggttgtag aacgccatac tgtctttttt
     1981 tgcggcggct ttttcaaacc atttgcgtgc ttcattgtag tcggcggtca taccctgtcc
     2041 gaagtaatac atgctgccca gattggtttg ggctttgctg tggccgagtt ctgcggcttc
     2101 tgtgtagagt ttgagtgcgg ctgcatattc gggcgcgcat tgtaatctgt aatgcagggc
     2161 ttgggctttt tgataaagcc gttctgccct ttgccgcctg atggcggaat ctttaatgcc
     2221 gccgtattgg ttaggatggt ttaacgcgct gtcggatatg agtttctgat aggcggaaag
     2281 gctgccggct tcggctgcct gccgataata atgccgggct ttttccgtat cgggggctat
     2341 gcccaaactg tagcggcaga tgtcgcccag tatccgcaaa ccttcgggat ggcgttcggc
     2401 ggcggctgct tcggcatgat gcagggcaag tttgtggttt ctttccagat gcttgccttg
     2461 taggtagatg tcggcaagtg cggcatgggc atcggggtca ttttcctgtg ccgcctgttg
     2521 gaaccggtaa acggcttcat cgggagccgt aggtaggatg agttcggcaa gcggggtgta
     2581 ggcgggaatg tatccttgtt gtgcggcggc gcgcaaatga ttgatagctt gggtcgtgtc
     2641 ggttggtgta ccttgtccga aacggtattg aaggccgagc tgccattgtg ccgccgccaa
     2701 gccttgtgct gcagcctcgc ggtagagaag gtgtgccgtt tcaaaatccg gtggcgtacc
     2761 gtactggcag tattgggcaa gcagatattg tccgtggggg ttgttgtttt ttgcggtcgt
     2821 ttttgcccat ttcagggctt cttcaaaatg tcgggacgca tacagaaggt acatcaggcg
     2881 gacttgtgct tcgggcagtc cttcctctgc caacggtctg taaagcggta tcagggtttc
     2941 cgggcgcgcg ccttccaatt cttggatata tgccagattg taacgcgcgt agggatgccc
     3001 ctgttcggcg gcgatgcgga gcatttccat accttgttcg cggtcttggt gcgggctgtc
     3061 cgcgtccatc aggtgttttg ctaactcgaa tgcggcggcg ggatgacctg ccgaggccgc
     3121 ttctgtcagc aggcggcgga cttcggtaaa gtcatattgt tcttgagaca ggcgttctaa
     3181 ggcttgatga tagagtaagt cgggattgtt catatcagat cgttttccgt tttgtcaaat
     3241 catcgttatt cgttatgaag ttaggtttat atcggcacgc ctgcctactg tatcatattt
     3301 tttacagacg gattttgtct ttgaaagcat gctaatgcag atgctcgtaa atggtttccg
     3361 ccaatgcctt gctgatgcct tcgacttgtt ccaaatcttc tttgctggcg gcaacgacac
     3421 cgcgcaggcc gccgaagcgg gtgagcaggg cctggcggcg tttgctgcct acgccgggga
     3481 tgtcgcttaa ggaggaggta acgcgtgctt tgtcgcgttt tttgcggtgg cctgtaatgg
     3541 caaagcggtg tgattcgtcg cgcacggttt gcaatagatg caaggccggg ctgttgggcg
     3601 gcaggcggaa gacttcgccg gtaaaaggca gtatgagttc ttccataccg gctttgcgct
     3661 cagggccttt ggcgatgccg accaagggaa tgtgcagacc gagttcttcc catacggata
     3721 cggcgacgcc gatttgtcct ttgccgccgt caatcaatac gacatccggc catttgacgg
     3781 tctcgccgtt ggcttcggct tcctgcattt tgccgtaacg gcgcatcaat acttcgcgca
     3841 tggcggcgta gtcgtcgccg ggcttggcgg tcgtgatgtt gtagcggcgg tattgcgaag
     3901 gctggatgtt ttgctcatcg tacacgacgc aggacgcaat agtggcctcg ccttgtgtgt
     3961 ggctgatgtc gaaacattcg aggcggttga ggccgtctga atccataccg aggattttgg
     4021 cgagttcgtc gatacggtgt tgctggctgc tttgttgcag gcggcgttgg gcaattgcca
     4081 tttgtgcgtt ttgctccgcc attttcaacc agactttgcg ttcgcctatg gttttggtaa
     4141 cgaactgcat ttgtttgccg tgttcgctct ctagggcttc tttcaatgcg tcgggcatgg
     4201 ggaagttgct gatgatgatg tcgggtttgc ttttgcccag atagtgttgg gcgacgaagg
     4261 cttcggcgta gtcttgtccg tttggttcgg gatcgttttt ggtgtcaggg aaaaaacttt
     4321 tatcgccgac gtgccgtccg ccgcggatgc tgacccagtg tacgcagacg atgccgtctg
     4381 aaaccgccag cgcgagcagg tcgatatcgt tgggattgtt cggattttta ctgtcgataa
     4441 actgattact ctgcatgatg ccgagtgctt ggatttggtc gcggtagcgg gcggcttctt
     4501 caaactgcaa attcgcggcg gcggtctgca ttttgtgttg cagggtgcgg gtcagttcgt
     4561 cggtcttgcc gttgagaaaa gtcgaggcct cgcgcacgct gtcgtggtag tcttcttcgc
     4621 tgatatggcc gacacagggt gcgctgcagc gtttgatttg atacagcagg caagggcggt
     4681 cgcggtgttc gaacacgctg tcttcgcagg tgcgcagcat gaagactttt tgcaggactt
     4741 ggatgctgtc gcgcacggcg ttgctgttag gatatgggcc gaaatattga ttaggctttt
     4801 tcagcgtgcc gcggtaatac gccatttgcg gatattgatg gccgctgagc atcaaataag
     4861 gatagctttt gtcatcgcgg aaaagaatat tgtatttggg cgacagggct ttgatgaagt
     4921 tgttttcgag aatcagggct tcggattcgg agcgggtgat ggtggtttcg atgtggtgaa
     4981 cctgtttcac catcaatgcg atgcgcgggg aatggtcgtt tttctggaaa tagccggaca
     5041 cgcgccgctt gaggttgacg gctttgccga cgtataagac gttgccgctt tcgttgaaaa
     5101 aacggtatac gcctggcagg ttgggcaggt ttttgaggaa aagtgggatg tcgaagtgtt
     5161 ccggaaaacg ggtttcttta ttcatatcaa ttacttgaaa aacggtttca gacggcatat
     5221 taccatatcg ctggagacag ccttatccgt tcggacaagg ctgcagggcg gttacatgct
     5281 gctaatcgat ttttacccgt ttcaggcgca aggcattgct caacacggaa accgagcttg
     5341 ccgccattgc cgcgccagcg atgacgggat ttaaaaagcc aagcgcggcg agaggaatgc
     5401 ccaagatatt gtagaagaag gcgaaaaaca ggttttgctt gatgtttttc aaagtggctt
     5461 gcgacaccag cagagcatcg gcgagttggt tgaccgaatg ctgcatcagc gtggcggatg
     5521 cggtatgttc ggcaacgtcc gctccgcctt tcatggcgaa gctgacgtta gcggcggcaa
     5581 gcgcgggcgc gtcgttgatg ccgtcgccga ccatcgccac ggttttgccg gcggctttga
     5641 gtttttgcac ttcggcagct ttatcgcgcg gactcatgtt gccgaaggcg tgtgcgatgc
     5701 ccagttgttt ggcgacgtat tcgaccgtgc cttggttgtc gccgctcata atatagacat
     5761 cgatattgtg ttttttcaga cggcctatgg cttcggcggt atcggctttc aacgcgtcgg
     5821 caagtgcgaa tgcgccgatg ggtttgttat cgaccgagac tgcaacaatg cttgcaatgt
     5881 cccaaacgcc gtctgaaaac tcgggcaagg taagttcggc aaattcggct ttgcctgctt
     5941 tcaccaaacc cacgtcttcc acttcggcgg caatgcctgc gccgacaacg gtttgagcgt
     6001 tttgtgcggc agggatttcc aagctgcgcg tttgggcggc ggaaacgatg gcacgggcaa
     6061 gcggatgagt ggcgttttgt tcgacggcag cggcgatgcg gtacaaatcg tcttcgtcaa
     6121 agccgctgtc gggaacacaa taaacggcgg caacctgcgg cctgccttcg gtcagcgtgc
     6181 cagttttgtc caacacaacg gcatcgacgt gggcggcttc ttccattgcc gctgcgtctt
     6241 taaaccaaat gccgtgtttc actgctttat ccatgccgac cataatcgcg gcgggtgtag
     6301 ccagaccgag cgcacacggg caggcaatca ccaaaacggc gacggcgtgc atcagcgcaa
     6361 ccgtccaatc gcccttaatc agccaagtaa caataaaagt caacaacgca atgcccacga
     6421 cggcaggcac gaataccgca gccgctttat cggctacgcg cgcaatcggt gctttactgc
     6481 cttgtgcttc agagagcgcg ttcatcatgt cgccgagctg ggtttggctg ccgagctgcg
     6541 tggcgcggta caccacactg ccttcggtca ttaacgcgcc cgccaacact ttgccgcccg
     6601 cctttttttc ttcaggattg gattcgccgg taagatggct ctcgtccgcc caaccgctgc
     6661 cgctttcaat gatgccgtct gcggcaatgc gttcgccgtg gttggcgcgg ataaggtcgc
     6721 cgatttgcac ttggtcgatg ggaagctgtt tccattcgcc gttgcgttgc acgttgactt
     6781 gggttggtgt aagtttcaaa agcaggccca agctgttcag gctggatttt ttggtacggt
     6841 gttccaaaaa tttgcccagc gacacaaaac cgatcaccat cacgcctgct tcaaaataca
     6901 catgcgccat gccgtgtgcc gcatgcggac tgaaaaataa catatagacg gaatacagat
     6961 aaatcgatac cgtgccgatg gtaacgagca cgtccatatt cgccaatcca cctttaatac
     7021 tcgcccacgc gcttttgtaa aacgggattg tcagccaaag ctgcaccaca ctcgccaatg
     7081 cgaactgcca caacggcgga atcatccaat cgtgtctgcc gatcatcatc cccgccatgc
     7141 cgataaggaa cgggacgttg atggtgaaca gcagccacag ccgccagccg atatggtgtt
     7201 ctgcttcggg ttgcggcaat gtatcttccg ttttttcctt cgcgccgtaa ccggttttct
     7261 caatgatttt ggcaatgtcg gctactgagg ttttgctgtc gtcaaacact acctgcgcct
     7321 cctcgctggc gaagttcact cccgccgatt cgacaaaatc ttttttgttc aacacttttt
     7381 caatacgcga agcacatgcc tgacaggtca tgccttcgat ttggaaacgg attttttgtt
     7441 gcataagcgt gattcctgct ttgatattca gacggcagtc aaggttgcgg gatgccgtct
     7501 gaacgcccgt atggtcgaaa tttctcaaac gcgccgataa ttcgagtaga atccgaaaac
     7561 ttgacacagc aaaggaaaaa caatgtccca acaccgcaaa ctgattattt tgggttccgg
     7621 ccccgccgga tacaccgccg ctgtctatgc cgcgcgcgcc aatttaaacc ccgtcattat
     7681 tacaggtatc gcgcaaggcg ggcaactgat gaccaccacc gaagtggaca actggcctgc
     7741 cgatgccgac ggcgtgcaag ggccggaatt gatggcgcgg tttctcgccc acgccgaacg
     7801 tttcggaacg gaaatcattt ttgaccaaat caacgccgtc gatctgcaaa aacgcccgtt
     7861 tacactcaaa ggcgatatgg gcgagtacac ttgcgatgcc ctgattgtcg caacaggcgc
     7921 gtccgccaaa taccttggtt tgccgagtga ggaagcgttt gcagggaaag gcgtttccgc
     7981 ttgtgccacc tgcgacggtt tcttctacaa aaaccaagat gttgccgtag ttggcggcgg
     8041 caatacggca gttgaagagg cactctacct tgccaatatc gccaaaaccg ttaccctgat
     8101 ccaccgccgc agcgagttcc gtgccgaaaa aatcatgatc gacaaactga tgaaacgcgt
     8161 ggaagagggc aaaatcatcc tcaagctgga aagcaacctg caagaagtac tgggcgacga
     8221 tcggggcgta aacggcgcat tattaaaaaa caacgacggt tctgagcaac aaattgccgt
     8281 cagcggcatt tttatcgcca tcgggcacaa gccgaatacc gatattttca aaggtcagtt
     8341 ggaaatggac gaagctggtt acctgaaaac caaaggcggc acggcggaca atgtcggcgc
     8401 aaccaatatc gaaggcgtat gggcggcggg cgacgtaaaa gaccatacct accgtcaggc
     8461 gattaccagc gcggcttccg gctgtcaggc ggctttggac gcggaacgct ggctgggcag
     8521 ccaaaacatt tgaatgccgt ctgaaaccga aattttcggc ggaatgcaaa tttttcgcac
     8581 caaatccttg attccgtcgg caaaccccaa tataatgcct gctctttgaa ccttgccttc
     8641 tgctttcgca tggcagaagg ctgtttttac catccataag gagataacat gcgtcattac
     8701 gagatcgtgt ttatcgttca tcctgatcaa agcgagcaag tgcctgctat ggttgaacgt
     8761 tacaaaacca tgattgccga agcaaacggt aagattcacc gtttggaaga ttggggtcgc
     8821 cgccaactgg cttacccgat taacaaaatc cataaagccc attacgtttt gatgaacatc
     8881 gaaaccactc ccgaagtggt tgaagagttg gaaaccgcat tccgtttcaa tgatgcggta
     8941 ttgcgccatc taaccatcaa aaccaaacac gccgttaccg aagcatcccc tatgttgggt
     9001 ggtgaaaagg ctaagaacct gctgagcggt gcgtctgaag aagcggctgc ccaataattg
     9061 ggattcaata atcttgtttc gcttgccgcg ttgattgaaa aggttttccc tattcgatat
     9121 acgcctgccg gaatctctgt cttagatatt attttaaagc atgaatcatg gcaggaggaa
     9181 aacgggcagc aatgccttgt ccaattggaa attccggcac ggattttagg caggcaggcg
     9241 gaagagtggc agtatcggca aggtgtatat gttcacgtcg aaggtttttt agctcaaaaa
     9301 agcagacgtt cccttatgcc gatgctcagg atacaaaata ttcaagaata taaaggttaa
     9361 acgacaatgg ctcgtcaatc attcaaacgt agaaaattct gccgtttcac ggctgaaaaa
     9421 atccaagaag tcgattacaa acaagttgat ttgctgaaag actttatctc tgaaaacggt
     9481 aaaatcattc ctgcacgcat cacaggaacg aaggcattct accaacgcca attggctgtt
     9541 gccgtaaaac gcgcacgctt cctggctctg ctgccttaca ccgaccaaca caaataattt
     9601 tggagattga atcatgcaaa ttattctgtt agaaaaaatc ggcggtttgg gcaacttggg
     9661 cgacatcgta accgttaaaa acggctatgc ccgcaacttc ttgattcctg ctggtaaggc
     9721 aaaacgtgct actgaagcaa acatgaaaga gtttgaagca cgccgtgccg aattggaagc
     9781 caaacaggcc gaaattttgg cagatgccca agcccgtcag gaaaaattgg atggccaaac
     9841 cattaccgtt gcacaaaaag ccggtgtgga cggtcgtctg tttggttccg ttaccaatgc
     9901 tgatattgcg gctgcaatcg ttgcgactgg catcgaagcc gtgaaagcaa atgtacgtct
     9961 gccgaacggt cctctgaaag ccgttggcga gtacgaagtg gaagtggctt tgcacactga
    10021 tgccgttgct aaaattaccg ttgccgtcgt tgccgcaacc gagtaattga aattatcaga
    10081 tgccgtctga aaccctgccg attgtcggaa tgtggtttca gacggtattt gttttgtttt
    10141 ccgtatattc tgcaaaggtc tcggtcaggt tgtctgtcct tttgtcggaa caggagactg
    10201 ggcagctttg tcctgattaa ttacttgtgc aaacggtaaa aaggtttttt cgtgtgtttt
    10261 aaagcaggtt gtacagcatt tttccggcaa tcaaaagcaa cataatgccg aaggattttt
    10321 tgagtttggc agaagaaagt ttgtgggcgg ttttgacacc gagcggggca aaggcaatgg
    10381 ttgccgcgct gaggacggcg acggcgggca ggtaaaggaa gcccagtgac ccttcgggca
    10441 atcctgcaat attcaggccg ttgagcagat acgatattgc gccggagagt gcaatcggcc
    10501 aggcaaggcc ggatgatgtg ccgatggctt tatgggcggg gaagccgcag tggattaaga
    10561 aggggacgga aagtgaaccg ccgcctatgc cgacccagct cgacattgtg ccgaacagtg
    10621 tggaaaccgc agtcagtccg ggcagtccgg gcagcgggcg ggatgccgtc tgagggtcgg
    10681 tatgcagtgt tttgaatgcg acggcggtta aaaacaggat gaagaaaatt tgaagcccga
    10741 acgctgggat atattttgcg gagagtgcgc cagcgaatac gccgaatacc atacccggca
    10801 tcatcgtaaa tacggttttc cagtcgaccg cctgtttttt gtgctgcccc agcatactgg
    10861 aaaaggcggt gaagaccatg acggcgaagg atgtgccgac ggcgaggtgt tgcgcgtaag
    10921 gatgttgtgc caaaccctgc aaatcaagca cccataaaac gacagggaca atcagcgtgc
    10981 cgccgcctac gccgaacagg ccggcaataa aacctgccgc actgcctacg gcaagcagga
    11041 ttaagataat gtcccaatgc cacatttaat ccgcctttct gtattttttc cagaatcggc
    11101 gtgccgccat aatgtagccg gacaggctgt atccgagaaa gaacaggaag aggacgagcg
    11161 acggttccca agtgaccaga agcagcagta agacggcaag cagcattccg acaaagggga
    11221 cttgtctgcg gatgttgatt tctttaaaac tccaaaaagg gatttggaca atcatcgaca
    11281 ggccggcaaa cagtgtgatg cccaatgccc accagtggac ggcggggaat ttttcgacgc
    11341 tgtggttgac ccaaatcagc ccgacaatca gcgcggcggc agtcggactg ggcacgccga
    11401 taaaccagcg tttgtccacc ttgccgatga gcgtgttgaa cagcgcaagg cgcagggcgg
    11461 cgcaggcgca gtagatgaag gcgacggaat aaccgatttt gccgaactgc caaagctgcc
    11521 atttgtaggc aatcagagcg ggagcaacgc cgaagctgac catatcggca aggctgtcga
    11581 gctgctcccc gaacgcgctt tggctgttgg tcagccgcgc cacgcgcccg tccataccgt
    11641 ccagcaacat agagatgaat accgcgatgg cggcggtttc ataacgtccg tgcatggatt
    11701 gggtgattgc gtaaaatgcg gaaaacagcg cggcgatggt aaaggagttg ggcagcaggt
    11761 agatactgtt ttgacgcagg gagcgttgcg gggtgttggt cggggtttcc atagctgaag
    11821 tgtctcacgg acgtggagaa tcgatagggg attataaccc aaaccattat cgaacaatac
    11881 gccctttagg atgaagccct cggaggcggt ttgtttttct gatggcggat gctttgcggg
    11941 gcggtttgac tctgcctgcc accgggtgtg ttgtttcgga tttttgccgg atggatattg
    12001 ttgccgggca tttctgggct tgccggaagg tatcgggggg agggcatcgg aatatgcggc
    12061 gcaccgttca aaccggggcg gcgtttccct attttcgggg agtttgcagg cttcaacaaa
    12121 ttgaaagata ttttttgcat ttccgaccgt atgctgattt tgaaacggga taaattatag
    12181 gacaaggttt ttcgcccgcc gcaaaggttg gccgcaatgc cgtctgaaaa caacggcttg
    12241 ccgctaaggc ggcggtatgc ggatttgcgc tgctgaaatc ggtttgccat acccggcggt
    12301 gcaatctgcg gggagggata agccgataag gcgggactta tcctgttttt tgtttataat
    12361 gcaacagaat ttggacgaac cgtcgggaat ttatcatgat gaaaaagata ctggccgtat
    12421 cggcactatg cctgatgact gcggcggcac gggctgccga tacttacggc tatctcgccg
    12481 tttggcagaa tccgcagaat gcggacgatg ttttgcaggt taaaaccaca aaagaagatt
    12541 cgacgaaaag cgaagcgttt tccgagttgg aagccttctg caaaggtcag gacacgcttg
    12601 cgggcattgc tgaagacgag ccgaccggat gccggtcggt cgtgtcgctg aacaatacct
    12661 gtgtcgcgct ggcatacccg aaagccttgg gcgcgctgcg tgtcgacaac gccgtcgtga
    12721 ttacttctcc gcgttttacg agcgttcatc aggtcgcact caaccagtgc atcaaaaaat
    12781 acggcgtaca gggacaatgc ggcttggaaa cagtgtattg cacatcttct tcttattacg
    12841 gcggaactgt gcgctctttg attcaaaatc tcaaataaaa cggaaaatgc cgtctgaaag
    12901 atgttcagac ggcatttcta tatcgacggt caggattctt tcggatcggg cagcaggctg
    12961 ttcaacataa tggcaagtac ggcgcacaag cccacgccgg caaagctgaa gctgcccaat
    13021 ttgagcgtca tgccgccgat gcccgtggtc agtaccgagc tgacgatgac caggtttttc
    13081 ggcagcatca aatcgacttt ggcatcaatc agcgttttca cgcccaaaga agcaatcgtg
    13141 ccgaacagca gcagcataat gccgcccatt actggcatcg gaatggaagc caaaaacgca
    13201 ttgaatttgc cgaaaaacgc catgcagacg gcaaaaaccg ccgcccaagt catgatgacg
    13261 gggttgctgt ttttggtaat catcaccgca cccgttactt cgccgtaggt cgtaaccggc
    13321 gggccgccga tcagacccgc aacgcacacg cccaaaccgt cgcctgcaag ggttttgtcc
    13381 aagcccgggt ctttcgtata gtctttcccc gtcacattgc cgattgccat gatgccgccg
    13441 atgtgttcga tggcgggggc gacggcaacg ggcagcataa acagtgccgc ctgccagttg
    13501 acctgaggcg tttcaaaatg gggaacggcg aaccagggcg cgtgtgcaat gcttgccgtg
    13561 tccaccagtc ccatcagcag tgccaaaaca taacccgaag cgacaccgat caagatagga
    13621 atcagcttca tcatcctgct gccgaaaacc gatacgatgg cggtaacggc aaaggtaaag
    13681 ccggaaagaa tcagtgaatc ggtatagtcg atgacctgtt tgccgtccgc ctgacccatt
    13741 gccatgctgc ttgccgccac ggcaacagac agaccgatga ccatgatgac ggggccgatg
    13801 acgaccggcg gcagcagttt gtgtaccgcc gccagtccgc gccaacgaat tagcacggca
    13861 aacacaaaat acataaagcc ggcggcaaac agtccgaaca tggtggaagg cagcccccat
    13921 tctccgatgg agtagataat cggtgcgata aaggcaaacg aagaaccgag aaaaatcggc
    13981 actttacgtt tggttgtaat ttggaacagc agcgttccca agcctgcgcc caaaagcgca
    14041 agagccggat tcagaccggt cagcagggga accagcacca ttgcgccgaa tgccacaaat
    14101 aaaatctgtg caccggaaac ggcaagtttc agttggttca taccatcttc tttcaatcgg
    14161 ttcaaacggc acggattata ctggtttgcc gagggtctgc gtaaaaatac atacgtcggg
    14221 caacagatat gccgtctgaa aggcaatgtc tgtctgttgc aggatgaaag caggagaaat
    14281 ggagtataat ccgacacttt ccatattcaa tccgcaagcc gtccggcttc cttatttcag
    14341 acggcatttt ccggtaatac ggcatgacag aaaaatccca taaaaaaaca gcaaaaggca
    14401 gggcgggaag cccgtcccca acgtccgcac gcaacaagaa agccgacaac ggcgcacggg
    14461 gcaataaagt ttccgagcgg ctcaaggcgg tcaaagagtt gcagaaaacc gaaaccaaaa
    14521 aggcgcgtcc cgaacatgtc gtcaacctta tcggcgacgc actgtggctg atgggtttgg
    14581 cggcaaccct gtatttggcg atttccctga tcagtttcga tatgggcgat ccgtcttggt
    14641 cgcacagttc gccggttgtg gaagatgtcg ccaattgggg cggactgttc ggcgcgtatg
    14701 ttgccgatgt cggctattat cttttcggct ggtcgttctg gtggtggata gcggctgcct
    14761 gcgtcgtgct gtataaaaat ttccgcctgc acgcaaaaca gacggaaaac gaggcataca
    14821 accacaaaat cgctgccgcc gcgctgtttg tcctgacggt cttcagcccc gtcttggagt
    14881 attttgtgct gggcggaaaa tatgccgact ccctgcctgt cggagcaggc ggtatggtcg
    14941 gcatacgcgt cggcgcagtg tttgcgtggc tgctggggaa atcgggcagc ctgctgatta
    15001 tcttggttgt tctgctgttg tcgttgtccc tactggtgca gatttcatgg ctggaatttt
    15061 tgaacggtgc aggcagggcg gttcaaaacc gcctgagtgc cttatccggc aaggtcatgg
    15121 ctttaggaaa acgccggccg aataccaaaa cagacggtgt cgatacccaa aatacacggc
    15181 gcatggtaaa agaagccaag aatattacgg ccaaacccgt tgccttgccc gaaggcagca
    15241 gcagcaaccg caaatccgtc gcggtttccg tcgcgccgcc gcccaaaatt caggtttctc
    15301 tgtttgaaga tgacgaacct cggcaggcgg gcgaatacca caagcctaca ttgaacctat
    15361 tgcggattcc cgacagcgag cccgtcagca tcaatcccgc cgaattggag cgcactgccg
    15421 aactaatcga atccaagctg gcagaattcg gcatcggcgt acaagtcgta tccgccacat
    15481 ccggccccgt catcacgcgc tacgaaatcg aacccgcgca aggtgtcaaa ggcagccaaa
    15541 ttgttgcctt gtcgaaagat ttggcacgct ctatgtcgct gcaatccgtg cgtatcgtcg
    15601 aaaccatcgc cgggaaaaac acgatgggca tcgagttgcc caacgacaaa cgccaagacg
    15661 tgatgttgag cgaaatcttg tcctcgcccg tgtttgccga agccaaatcc aagctgaccg
    15721 tcgcgctggg caaagacatt gccggtaccc ccgttgtcgg cgacttggcg aaaatgccgc
    15781 accttttggt cgccggtatg actggttcgg gcaagtccgt cggcgtgaac ggcatgatta
    15841 tgtctatgct tttcaaagct acgcccgacg aagtccgctt cattatgata gacccgaaaa
    15901 tgctcgagtt gagcatttac gacggcattc cgcacctgct ctgtcccgtc gtgaccgata
    15961 tgcgcgaagc agggcaggcg ttgaactggt gcgtcgccga aatggaaaaa cgctaccgcc
    16021 tgctttccca cgccggtgta cgcaatctgg aaggcttcaa ccaaaaagtc gaagccgcaa
    16081 aagcggcagg caagcctttg cttaatccgt tcagcctgaa ccccgacgag cccgagccgc
    16141 tggaaaaact gccgttgatt gtggtcgtta tcgacgaact tgccgacctg atgatgaccg
    16201 aacgcaaagc cgtcgaacag caaatcgccc gtctcgccca aaaagcgcgc gccgccggta
    16261 tccatatgat tgtcgcgacc caacgtccca gtgtcgatgt cgttaccggc ctgattaaag
    16321 ccaacatccc gacgcgtatg gcgtttaccg tgcaaagcaa aatcgacagc cgtaccatcc
    16381 tcgaccaaat gggcgcggac gaactgctca aatatggcga ttcgctgttc cttcagcccg
    16441 gcagtgccga accgacccgc ctgcaaggcg cgtttgtttc agacgacgaa gtacatcaag
    16501 tcgtcaacta cgtcaaatcg caagccccag ccgactatat tgaaggtctg ctcagcggcg
    16561 aggccgcgct ggaaactgcc aatatcgtta atccgaatgc agacagcgac gaattgttcg
    16621 atcaggcagt cgcctatgtt ttggaaagca aaaaaacctc catttcgtct ttgcagcggc
    16681 agttgcgtat cggctataac cgcgcggcaa acctgatgga ggcactggaa aatgcgggtg
    16741 tcgtttctcc caccgacctc aacggcagcc gtaaaatttt ggcgcacaag gaccatttgt
    16801 agcccgtatt gcaaaatgcc gtctgaacgg cggaattggc gtttcagacg gcatattatg
    16861 tttcaggcga aacattgtga tatacttgcc agctaaaatt tcccctttgc ggcaatgcgg
    16921 ttcaaatatc gtaccgttgc gctgtttgct tccccatgta gggaagaaag tttatcattt
    16981 tatcaacaca acaaatttaa gggcttatga tgagcgtaac tgttgaaact ttagaaaatc
    17041 tggaacgcaa agtagtgttg tccctgcctt ggtccgaaat caacgcagaa accgataaaa
    17101 aactgaaaca aactcaacgc cgtgcaaaaa tcgacggttt ccgtccgggt aaagcacctt
    17161 taaaaatgat tgcccaaatg tacggtgcga gcgcacaaaa cgacgtgatc aacgagctgg
    17221 tgcaacgccg cttctacgat gttgccgttg cccaagagtt gaaagtggca ggcttccccc
    17281 gttttgaagg cgttgaagaa caagacgata aagagtcttt caaagttgcc gccatttttg
    17341 aagtgttccc cgaagtcgtt atcggcgatt tgtctgcaca agaagttgaa aaagtaaccg
    17401 cttccgtcgg tgatgccgaa gtggaccaaa ccgtagaaat cctgcgcaaa caacgtaccc
    17461 gcttcaacca tgtcgaacgc gaagctcaaa acggcgaccg cgtcatcatt gactttgaag
    17521 gcaaaatcga cggcgaacct tttgcaggcg gcgcatccaa aaactacgcc ttcgtattgg
    17581 gcgcaagtca aatgctgcct gaatttgaag ccggcgtagt cggcatgaag gctggcgaaa
    17641 gtaaagacgt taccgtcaat ttccctgaag actaccacgg taaagacgtt gccggtaaaa
    17701 ctgccatgtt caccattacg ctgaacaacg tttccgaagc gactctgcct gaagtcgatg
    17761 cagattttgc aaaagccttg ggtattgcgg atggcgacgt tgccaaaatg cgcgaagaag
    17821 tgaagaaaaa cgtaagccgc gaagtggaac gccgcgtaaa cgaacaaacc aaagaatccg
    17881 taatgaacgc gctgctcaaa gccgtagagc tgaaagcacc tgttgctttg gtaaatgaag
    17941 aagccgcacg cttggcaaac gaaatgaaac aaaattttgt taaccaaggt atggctgatg
    18001 ctgccaactt ggatctgcct ttggatatgt ttaaagaaca agccgaacgc cgcgtgtctt
    18061 tgggtctgat tttggccaaa ctggttgatg aaaacaaact ggaaccgact gaggagcaaa
    18121 tcaaagccgt tgtcgccaac ttcgcagaaa gctacgaaga tcctcaagaa gtaatcgact
    18181 ggtactacgc agagccttcc cgcctgcaag ccccgacttc tttggcgata gaaagcaacg
    18241 tcgttgattt cgttttgggt aaagccaaag taaacgaaaa agctttgtct tttgacgaag
    18301 tgatgggcgc gcaagcctga ttatcctgaa aatgccgtct tagggcagtt tttgaaagca
    18361 ccgaagccgc ttttaaagcc cgctttggtg cttttacatc atgaaaggag acgaaatgtc
    18421 ttttgataac tatcttgtcc ctaccgttat cgagcagagc ggtcgcggtg agcgtgcatt
    18481 cgatatctat tctcggcttt tgaaagagcg catcgtattc ttggtcggcc cggtaaccga
    18541 cgagtccgcc aatctggtgg ttgcccaact gttgtttttg gaaagtgaga atccggataa
    18601 ggatattttc ttctatatta actcgccagg cggttcggta acggccggta tgtcgattta
    18661 cgacaccatg aattttatca agcccgatgt atcgactttg tgcttggggc aggcagcaag
    18721 tatgggcgcg ttcttattgt cggcaggcga aaaaggcaaa cgtttcgccc tgcccaacag
    18781 ccggattatg attcaccagc ctttaatcag cggcggcttg ggcggtcagg catccgatat
    18841 tgaaattcac gcacgcgaac tgttgaaaat caaagaaaaa ctcaaccgcc tgatggcgaa
    18901 acattgcggc cgcgatttgg cagatttgga gcgcgacacc gaccgtgata atttcatgtc
    18961 cgccgaagag gctaaagaat atggtctgat tgatcaaatc ttggagaacc gtgcttcttt
    19021 acagttttaa taagaaatct gagagaaaca aatgccgtct gaaaacagtt caggacggca
    19081 tttgtcttat agggactgta tagggatgat tttgttctgg ttgcgtttgg agccgttttc
    19141 accatagttg gttttgacat attcctctac aatagctagg aattcagttg caatattatc
    19201 acctttcagt gtgactttgc gctccccgtc cacataaacc ggcgcgacgg gtgtttcccc
    19261 cgtaccgggc aggctgatgc cgatgtcggc caatttgctt tctccgggcc cattgacaac
    19321 gcagcccatt acggcaacgt tcagggattc aaccccggga taaagggtac gccatataga
    19381 cattttttgg cgcaggtaat tttgaacatc ttgtgccagc tcttgaaata cggtactggt
    19441 ggtacgcccg cagccggggc aggcggtaac catcggcgta aacgaacgca atcccatagt
    19501 ctgtaaaatc tcttgcccga cgacgacctc ctgagtacgc gggctgccag gttccggagt
    19561 cagtgaaatg cggatggtgt cgccgattcc ttcttgaagc aagacggata atgccgccgt
    19621 tgatgcgaca atgcctttgc tgcccatacc ggcttcggtc aaacccaaat gcagcggata
    19681 ggcgcaacgg ctgcccagtt cgcggtaaac ctgaatcaaa tcctgaaccg cgctgacttt
    19741 gcacgacagg atgattttgt cttcgggcag tcccaataga acggcttttt cggcagattc
    19801 caaagcggag acaatcagtg cttccttcgt cacttcttcg ggcggtttcg gcgcggaaaa
    19861 agcgaggttg gcatccatca tacgtttggc gaggctttga tccaaagaac cccagtttac
    19921 gccgatgcgg acggctttat cgttttcagc agcagtccga atcataaagg caaatttttc
    19981 atcgcctttt acgcctttgc cgacattgcc gggattgatg cggtatttgg acaatgcttt
    20041 gccgcattcg ggaaattccg ccaacaggcg ttcgccgttg aagtggaaat cgccgataag
    20101 cggtgtggca tagcccatat cgtccaagcg gcggcggatt tcggcaactt tggacgcggc
    20161 ttcggggctg ttgacggtaa tacgcaccat ttcggatccg gcatcgctca attccttaat
    20221 ctgcaatgcg gtggcttttg catcggcagt gtcggtgttg gtcatagatt ggataacgac
    20281 gggtgcttct gaaccgacgg taatatgatc gatgcggact tgatgcgtct tgcggcgttg
    20341 gagtgtgttc atatggtttg aatctgttta ttgaccggtg aggacggttt gcaattcttc
    20401 cgagtagggg aaattcgcct gcaattgtgc ttcatattcg tatgccgcct gtgcgttgcc
    20461 gagggctttg gcaattttcc agcctagcag caaatcatcg gcctgaagga cttctaccct
    20521 gctttggtat tttttaaagt agtaatcggc atcgcccaac tgcccggcca gcattttggt
    20581 gcgcgccagt tctttaaatg cgggtgggaa ctgcggctgg gcggcgaggg aacgtttcaa
    20641 ataggcttcc gccaatccga attgcccctg ttttgcgctg catatgcctt tattcaggtt
    20701 ggcaatataa ggggtcgggt aggtggggtc ggccagggct ttgtcgaaat atgccataga
    20761 ttcggcaggg cggttgagcc tgccgcacag gaaccagccg tagttgttgt tgatttcggc
    20821 actgtcgggt ttgatggaga gggcttgccg gaaactttcc tgcgccttgt cgttaacttt
    20881 caggtattga tagatttcgg cacggaccag ccaggcaagc tcgtttttag ggtctgattt
    20941 caaggcgtct tcaatacttg ccgtcgcctg acggtagtcc tgaccgcgca tatattccat
    21001 tgccaactgg gttttgatat tggaaacctg attggctttt tctgcccgcg aggggcggta
    21061 ggaagtgctg cacgcgccca aggcaaggac aagtaataaa gagattcgtt tggatggctt
    21121 aaaaggcata attacccctg ttgtccgatt aaaatctgct gccatttttg ttggcggcgc
    21181 gttttatcct gaacctgccc cgccaactgt ccgcaggcgg catcgatgtc gtcgccgcgc
    21241 gtttttcgta cggtaacgac aaatcctgcc tgctgcaaaa tatcgcggaa cacacggatg
    21301 ttctcattgc tggagcgttc gtatccggag tttgggaagg gattgaacgg aatcagattg
    21361 aacttgcagg gaacatctgt gaccagttcg atcagttcgc gcgcatgttg cgccttatcg
    21421 tttattccgt ccaacatgac gtattcgaaa gtgatgaaat ccctgggtgc tttgaccaga
    21481 tagcgttggc atgcggccat caattctttc aagggatatt ttttgttcaa cggtacgatt
    21541 tggttgcgga cttcgtcatt ggaagcgtgg agggaaaccg ccaaagccac cggcatgaca
    21601 tcgcgcaacc tgtccatttg gggaaccata cccgaagtgg aaacggttac gcggcggcgg
    21661 ctcaaaccgt agccgtggtc gtccagcatg atgcttaagg cggtaacgac attgtcgaag
    21721 ttcgccatcg gctcgcccat gcccatcatg acgacgttgg aaataacgcg ctcgtttttc
    21781 ggtgtaacgc ccatcgcttt gtttgcccac cacaattgcc cgatgatttc ggcagcagtc
    21841 aaattgcggt tgaagccctg ccgaccggtc gaacaaaatg tacattccaa agcgcagccg
    21901 acttgtgagg aaatgcagag cgtgccgcga tccgattcgg ggatgaagac ggtttccacc
    21961 ccgttgcccg taccgacatc caaaagccat tttcgagtgc cgtctgaaga tttttgagac
    22021 atcatcagct tgggaatttc gatgcctgcc tgttcgttca gtttatggcg caacgatttt
    22081 gccaaatcgg tcatttcgtc aaaattttgc gcgccggatt ggtgcatcca acgcataacc
    22141 tgtttggcac ggaaaggttt ttcgcccata tcggcaaaat gtcgggtcag cccttggagg
    22201 tcgtagttga gcagattggt tttcatgtat ttgtgttttt cttaaattaa ggcttcaaat
    22261 aaaaaattgc agggcaggca agtttgattt gaagcctttt gcaacagagt tttcagacgg
    22321 catggatata ttttatgccg tctgaagagg agttgtgcgg tgtattgtat caacgggggc
    22381 agatttcggt ttggctgaaa aagtaggcaa tttccaaagc ggcattttcc ggagtgtccg
    22441 agccgtgtac ggcattgatg ctgaccgaag tggcaaagtc cgcgcgtatc gtgccttcgg
    22501 cggcttcgga aggattagtt gcacccatca gttcgcggtt tttcagaacg gcgttttcac
    22561 cctctaatac ctgaatcata accggaccgc cggtcataaa ttcaaccaat ccggcgtaga
    22621 aggggcggtc tttatgaacc gcataaaatt cttgcgcctc tttgagggta agctgcttca
    22681 ttttggcggc aacgattttc agaccgttct cctcaaagcg gctgtatatt ttgccgataa
    22741 cgtttttgcc gacggcatcg ggtttgatga tggagatggt acgttcaatc gccatgctat
    22801 atccttattt ttacttaaga agaatcaaat cggtattcta tcaaaaaaat aacttatccg
    22861 cctgaagcct tgggagatag ggtcaggtgc ggctgttcca aataaacttc ggactgcatt
    22921 tcgaccatcc ggctggcggt gcgggtaaat tcttcggcaa aatcgccttc tgtatagata
    22981 tggttgacat ctaccgcgcc ggtggcacac agtttgacac ggaaatcgta gagtacgtca
    23041 atcagccaag tcagccgccg tgcctccgcc ttttcttgcg gtgagagttg ttccaaacct
    23101 gaaataaaaa ccatttcata atgttcggcc aaatacaggt agtcggactg tgagcggggg
    23161 ccgaagcaca gtgcgcggaa atcaaaccat atggcacggc cggactcggc tttgtgggga
    23221 atctcccgac cgtggatggt gctgatgccg gggttcaaat cggtaatgcc tgtcatttct
    23281 ttgaacagtt ttgccagttt tgcctcattt tcttcattgg caggcgtaaa gaaaatctcg
    23341 gcggggcgga gggtacgcag tcggtagtct tcaccgccgt caacatttaa gacggtcagg
    23401 ctggactcga tgagcgcgat tgtgggaaga aaactgctcc ggttttgacc ttgcgggtag
    23461 agttcggaag gcgcgtagtt tgaagtcgcc accaaaacaa cgccctcgtt aagcaggttt
    23521 tccagcagac ggcctaaaat cattgcatcc gcaatatcgc tgacatgaaa ttcgtcaaaa
    23581 cacaatacgc gggtttcttt ggcaatctcg gcggcaacgg atttcaacgg gttgctttcg
    23641 cttttcaggg ttttcagccg ccggtggatt tctgccataa aggcatgaaa gtggacgcgg
    23701 cgtttgcggc ggtaagggag gcagccgaaa aaagcgtcca tcagaaagct tttgccgcgt
    23761 ccgaccccgc catagaaata aagccctttg gggacttgcg gggaacgcaa actcctgcct
    23821 aaaaaacggt ttcttttgcg tttgaacatc atcaattcgg tccaaagccg atcgaggtgt
    23881 tcgatggcgg cagcctgcgc gtcgtcgcgg atgaagttgg gcagttgtga ggcagcctga
    23941 taccaggtca gcgggctgtg gttttcaaac ggcggggctt taaaaagttg gtctctacta
    24001 ctcatattta acctttttat acttcttgcg gctaagaaag aaaatgctta ctgtcggtat
    24061 tataaatgat ttccttcaaa cttggcattt tccgtggaaa agaataatga cgcgcacaag
    24121 ggggtatgag caaaggcggc gtttcaatat ggtgcagata tgagctcaaa tgccttttat
    24181 agt
//
LOCUS       DAXHMX010000012        22846 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHMX010000012 DAXHMX010000000
VERSION     DAXHMX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.012"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..246)
                     /locus_tag="ACFM4S_000363"
                     /pseudo
     CDS             complement(1..246)
                     /locus_tag="ACFM4S_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(310..717)
                     /locus_tag="ACFM4S_000364"
                     /pseudo
     CDS             complement(310..717)
                     /locus_tag="ACFM4S_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(950..1297)
                     /locus_tag="ACFM4S_000365"
                     /pseudo
     CDS             complement(950..1297)
                     /locus_tag="ACFM4S_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1511..1720)
                     /locus_tag="ACFM4S_000366"
                     /pseudo
     CDS             complement(1511..1720)
                     /locus_tag="ACFM4S_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1749..2078)
                     /locus_tag="ACFM4S_000367"
     CDS             complement(1749..2078)
                     /locus_tag="ACFM4S_000367"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6050117.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKESGKRKLTIPSEMAYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(2156..2374)
                     /locus_tag="ACFM4S_000368"
     CDS             complement(2156..2374)
                     /locus_tag="ACFM4S_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6050118.1"
                     /translation="MLKTLTFAALHFSTAFSITYVLTGSIGISGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            2496..3449
                     /locus_tag="ACFM4S_000369"
     CDS             2496..3449
                     /locus_tag="ACFM4S_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6050119.1"
                     /translation="MNQKKVVVLDADTLPGRTFHFDFPHELAVYGTTDAAETAGRVRD
                     AHIIITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIQDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPAVREGYVAFENAVRTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLKARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(3523..5580)
                     /gene="metG"
                     /locus_tag="ACFM4S_000370"
     CDS             complement(3523..5580)
                     /gene="metG"
                     /locus_tag="ACFM4S_000370"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6050120.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGKLKDVSDSALLAKLTAQSEAIAECYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTATN
                     AARFLNLDAITWTNTRVTLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATAEGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(5696..7534)
                     /gene="glmS"
                     /locus_tag="ACFM4S_000371"
     CDS             complement(5696..7534)
                     /gene="glmS"
                     /locus_tag="ACFM4S_000371"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6050121.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGIAGSIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKSGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATKGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            7640..7765
                     /locus_tag="ACFM4S_000372"
     CDS             7640..7765
                     /locus_tag="ACFM4S_000372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050122.1"
                     /translation="MPSEGLGLLKKGRDFPVYPRWAFRRHCLPPVYSFYKSILTS"
     gene            7816..8337
                     /locus_tag="ACFM4S_000373"
     CDS             7816..8337
                     /locus_tag="ACFM4S_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050123.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVVTGKEESMLLSEKDGELSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYRDARNALPSNQTYQQHLAAIEQLKRRFEAEFDELEK
                     EIKCNGRSPALLL"
     gene            complement(8587..8649)
                     /locus_tag="ACFM4S_000374"
                     /pseudo
     CDS             complement(8587..8649)
                     /locus_tag="ACFM4S_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8755..10080)
                     /locus_tag="ACFM4S_000375"
     CDS             complement(8755..10080)
                     /locus_tag="ACFM4S_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6050124.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            10291..11130
                     /gene="efeU"
                     /locus_tag="ACFM4S_000376"
     CDS             10291..11130
                     /gene="efeU"
                     /locus_tag="ACFM4S_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6050125.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIAVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            11127..12293
                     /gene="efeO"
                     /locus_tag="ACFM4S_000377"
     CDS             11127..12293
                     /gene="efeO"
                     /locus_tag="ACFM4S_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6050126.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            12562..13827
                     /gene="efeB"
                     /locus_tag="ACFM4S_000378"
     CDS             12562..13827
                     /gene="efeB"
                     /locus_tag="ACFM4S_000378"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6050127.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(13896..14228)
                     /locus_tag="ACFM4S_000379"
     CDS             complement(13896..14228)
                     /locus_tag="ACFM4S_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6050128.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNKTESAAAEAAEEV
                     RDANGAVLQNGDTIILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIACKIDGSAMNL
                     KSEFVKKA"
     gene            complement(14303..15673)
                     /gene="glmU"
                     /locus_tag="ACFM4S_000380"
     CDS             complement(14303..15673)
                     /gene="glmU"
                     /locus_tag="ACFM4S_000380"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6050129.1"
                     /translation="MPHNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKDQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLKTLLEAAGDKVGLLTDVPTDPTGLGRIIRDGNGSVTAIVEEKDANVA
                     QKAVTEINTGILVLPNAKLEAWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SYLAAGVNNKCQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVIIDAN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKISPFSHLEDCEVGENNRIGPYARLRPQ
                     AKLAADVHIGNFVEVKNAAIGNGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(15728..16000)
                     /locus_tag="ACFM4S_000381"
     CDS             complement(15728..16000)
                     /locus_tag="ACFM4S_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6050130.1"
                     /translation="MRKTFLFLIAAAALLSGCAWETYQDGNGKTAVRQKYPVGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPDQTDDSEQGVRGQNWQKPKFQNR"
     gene            complement(16026..16706)
                     /locus_tag="ACFM4S_000382"
     CDS             complement(16026..16706)
                     /locus_tag="ACFM4S_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6050131.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAITRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRKSHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHALPFIDTCARDMAQLARYAETLSER
                     RQNHYNTP"
     gene            16765..17766
                     /locus_tag="ACFM4S_000383"
     CDS             16765..17766
                     /locus_tag="ACFM4S_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6050132.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            17860..18708
                     /locus_tag="ACFM4S_000384"
     CDS             17860..18708
                     /locus_tag="ACFM4S_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6050133.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            18734..19219
                     /locus_tag="ACFM4S_000385"
     CDS             18734..19219
                     /locus_tag="ACFM4S_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6050134.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(19321..20889)
                     /gene="msrAB"
                     /locus_tag="ACFM4S_000386"
     CDS             complement(19321..20889)
                     /gene="msrAB"
                     /locus_tag="ACFM4S_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6050135.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLQGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            21035..22300
                     /gene="ftsY"
                     /locus_tag="ACFM4S_000387"
     CDS             21035..22300
                     /gene="ftsY"
                     /locus_tag="ACFM4S_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6050136.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            22545..22829
                     /locus_tag="ACFM4S_000388"
     CDS             22545..22829
                     /locus_tag="ACFM4S_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6050137.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT         4870 a         5616 c         6817 g         5543 t
ORIGIN      
        1 ttcgttgccg ctgtcggcgg tgacgtcggc gtcggcgtcg gtggctttgt cggcgcgcgt
       61 aaccggctgt ccgcagaacc attttaccga accggcttga cgcttggccc acagggagag
      121 ttttctgcct tggatttctt tgtttacgtt gcttgaagcc atttcggcgg taacgacgcc
      181 gtttttgact tcaactttct caacatattt gcctttgatg tcggaggcgg atgccacgcc
      241 ggcggcatca ttaaatcccg tcattcccac gaaagtggga atctaggact caggaccgga
      301 gaaacctttt tacccgataa gtttctgcgc cgacagacct agattcccgc tttcgcggga
      361 atgacgggat gtaggttcgt gggaatgatt ggtgtcgatc ttcttgttgg cggcggcggt
      421 aacggcgtcg gcgtcggctt tggcgcgctg aaccggctgt ccgcagaacc attttaccga
      481 accggcttga cgcttggccc acagggagag ttttttgcct tggatttctt tgtttacgcc
      541 ggttgatttc atttcggcgg taacgacgcc gtttttgact tcaacgcttt taacatattt
      601 gcctttgatg tcggtggcgg aggatgccac gccggcagaa gtattgtttt ttggccatat
      661 gccgtgattc aggtaatact cggtgacggc tgatttttga ccttcagcca aaagaatgac
      721 gaattttagg tttcttattt tggttttctg tctttgaggg aatgatgaaa ttttgagttt
      781 taggaattta tcgggagcaa cagaaaccgc tccgccgtca ttcccgcgca ggcgggaatc
      841 taggtctgtc ggtacggaaa cttatcggga aagcggtttc tttagatttt gcgttctgga
      901 ttcccacttt cgtgggaatg acgggatgta ggttcgtggg aatgacggtt taggtatttt
      961 tatagaaagc cgtaggtggt gtttctatgc aaacggcaga tgctgcgtcg cggcaggttg
     1021 acggcaggtg cttggtgttg attttgtcgg tgtcgggtgt ggcggcggtg acggcgtcgg
     1081 cggtggcttt ggcgttgcgc tcaaccggct gtccgcagaa ccattttacc gaaccgtttt
     1141 gacgcttggc ccacagggag agttttctgc cttggatttc tttgtttacg ccgcttgaag
     1201 ccatttgggc ggtaacgacg ccgtttgcga ctgtaacgct ttgaacatat ttgcctttga
     1261 tgtcggaggc ggtggatgcc acgccggcgg catcattaaa tcccgtcatt cccacgaaag
     1321 tgggaatcta ggactcagga ctggagaaac cgttttaccc gataagtttc tgcgccgaca
     1381 aacctagatt cccgcttttg cgggaatgac gaagtttttc tgccatttgc cgtgattcgg
     1441 gcaatactcg gtaacggctg attttttgga agtgtttgaa gtcggcgcgt ggtgtttcta
     1501 tgcaaccggt agatgaatca tcgcggcagg ttgacggcag gtgcttggtg ttgattttgt
     1561 cgtcggtgcc gttggcggtg gcggctttga cgtcgtcggt ggctttgtcg gtgcgcgcaa
     1621 ccggcagtcc gcagaaccat tttaccgaac cgtcttgacg cttggcccac agggagagtt
     1681 ttttgtcttt gatttcgttg tttacgccgc ttgaagccat tatgtcagac ggtattgccc
     1741 gggcagcttt attcgtacac tttcagcagc tcgacttcaa atatcaaagt ggcgtgcggg
     1801 ggaatcacgc cgccggcacc gtgtgcgccg taagccattt cagaagggat ggtcagcttg
     1861 cgtttgccgc tttccttcat tccgccgaag ccttcgtccc agcctttgat gacttgtccg
     1921 acgccgagcg tgatggtcag cggctggcgg cggtcgaggc tggagtcgaa tttggtaccg
     1981 tcttccagcc aacctgtgta atgcacggta atctctttgc ctttaactgc ttcttttccg
     2041 aagccttctt gcaagtcttc aataatcagg ccgcccatat ttgtcctttc attgcttgtt
     2101 ggtcaaaacg gcaagggtaa cataccgtcc gtcgaagtca aatgccgctc aaacgtcagc
     2161 tgcatcggtg cagctgaaac ggctgtgttt gttggactgt tttatttttt tcgtaaaggt
     2221 tccatgcttt ttcatggaaa tagaaaacga cggtgttgat taggggctcg accagcgcga
     2281 ccgccccgga tatgcctata ctgcccgtca gtacataggt tatactgaag gcggtgctga
     2341 aatgcagtgc ggcaaaagtc agggttttaa gcatcatcct ctcccgaatt ggacattgac
     2401 ggagggatga taaagattat cataaggctg tgcggtttaa atttgctatt tgctgttagt
     2461 gtaaataaat cattttctaa atagggataa aaattatgaa tcagaagaaa gtagtcgtat
     2521 tggatgcgga tactttaccc ggtcggactt ttcacttcga tttcccacac gagcttgcgg
     2581 tttacggtac gacagatgct gcggaaacgg cagggcgggt gcgcgacgcg catattatca
     2641 tcaccaataa ggtgatgatt tccgccgaca ttattgcggc taatcctcag cttgagctga
     2701 ttgccgtcag tgcgaccggt gtgaacaatg tcgatattgg ggcggcggag gcggcgggcg
     2761 ttgcggtatg caatgtccgc gcctacggaa acgaatcggt tgcggaacat gcctttatgc
     2821 tgatgattgc cctgatgcgg aatttgcctg cctatcagcg tgatgttgcg gcaggattat
     2881 gggaaaaatc gccgtttttc tgccattacg gcgcgccgat tcaggatttg aacggcaaaa
     2941 cgctggcggt tttcggacgc ggcaatatcg gacggacgct ggcaggatac gcgcaggcgt
     3001 tcgggatggg ggtggtgttt gccgaacaca aacacgcgcc cgctgtgcgc gaaggctatg
     3061 ttgcctttga aaatgcggta cggactgccg atgtgttgtc gctgcactgt ccgctgaacg
     3121 cacaaactga aaatatgata ggcgaaaatg agttgcggca gatgaagccc ggtgcggttt
     3181 tgattaattg cgggcgcggc gggctggtgg atgaaaacgc gctgcttgcc gcactcaaat
     3241 acgggcagat cggcggggcg ggcgtggatg ttttgacgaa tgagccgcct aaaaacggca
     3301 atcctctgtt gaaggcacga cttcccaatc tgattgttac gccgcatacc gcgtgggcaa
     3361 gccgcgaggc tttggacagg ctgtttgata tattgttggc gaacattcac gcctttgtga
     3421 aaggcgaggc gcaaaaccgg gtggtttgag cttgtcggga tgacgaaaaa atgccgtctg
     3481 aacgcctcaa gggttcagac ggcattgttt tgacatttct gcttaaccga ctttgtcgcc
     3541 cggttgtgcg cctgcatcca catccaagag cttcagtttg ccctctgccg tggcggcact
     3601 caaaatcata ccttcggata cgccgaattt tgccattttg cgcggggcga agttggcgac
     3661 ggcgatgacc attcttccgt ttaactcggc agggttggga taagacgcgg cgataccgga
     3721 gaagatgatg cgtttttcaa aaccgaaatc gaggtcgaat ttcaaaagtt tggtgctgcc
     3781 ctcgacggct tcgcagttca agactttggc gacgcgcata tcaattttca taaagtcgtc
     3841 gaagctggcc tgttcggcga ctttttcgta tttgctctct tcggcggcag gtgcggctgc
     3901 ggcggcgatg ctttgtttgt tggcttcgat taaatcgtcc acttgttttt gctccactcg
     3961 ttgcattaaa tgtttgtatt tgttgatggc gtgtttgccc aaggttaccc ttgtgttcgt
     4021 ccaagtaatc gcgtccagat tgaggaaacg cgcggcattg gtggcggttt gcggcaatac
     4081 gggggcgagg taggcggtca gcatcgtaaa ggcgttgatg agttcgctgc atacttcgtg
     4141 caggcgttcg tcttggcctt cttgtttggc gagttcccaa ggtttgttgg catcaacgta
     4201 ttcattaaca atgtctgcca atgccatgat gtcgcgcagg gcgcgggcgt attcgcggtt
     4261 ttcgtagcat tcggcaatgg cttcgctttg cgcagtcagt tttgccagca atgcgctgtc
     4321 ggaaacgtct ttgagttttc cttcaaagcg tttggcgatg aaacctgacg cgcgggcggc
     4381 gatgttgacg tatttgccga cgaggtcgct gttgacgcgc gagataaagt cttgcaggtt
     4441 caaatcgatg tcttcgattt tgctgttgag tttggcggcg atgtagtagc gcatccactc
     4501 ggggtttagg ccttgttcca gataggattt ggcggtaata aacgtgccgc gcgatttgga
     4561 cattttttgt ccgtccacgg tcaaaaagcc gtgtgcgtac acgccggtcg gggcgcggtg
     4621 gccggagaaa tgcagcatgg cgggccagaa cagggcgtgg aaatagagga tgtctttgcc
     4681 gatgaagtgg tacatctcgg tttggctgcc ggctttgaag tattcgtcga aatcgatgcc
     4741 gatgcggtcg cacaggtttt taaacgatgc catgtagccg acgggcgcgt ccagccagac
     4801 gtagaagtat ttgcccggcg cgtcggggat ttcaaaaccg aaatacggcg cgtcgcggga
     4861 aatgtcccag tcggacaggg tggtttcttc gccttcgccc agccattctt tcattttgtt
     4921 gagggcttct ggttgcagat ggggtttgcc gtcgtgcggg ttgttgccgg aagtccatgc
     4981 tttgaggaag tcggcgcatt cgcccagttt gaagaagaag tgttcggatt cgcgcaattc
     5041 gggtttcgcg ccggaaacgg cggaatacgg gttaatcagt tcggtcgggg aataggtcgt
     5101 gccgcagact tcgcagttgt cgccgtattg gtcttgggcg tggcatttcg ggcattcgcc
     5161 tttaacgaag cggtcgggca ggaacatttg tttttcgggg tcgaaaagct gctcgatgac
     5221 gcggctctca atcttgccgt tggctttcag cgcgcggtaa atatcttggg aaaactgttt
     5281 gttttcaggg gaatgggtgc tgtaataatt gtcgtagccg atgttgaaac cggtaaagtc
     5341 ggcgaggtgt tcttcgcgca ctttggcaat catgtcttcg ggcgcgatgc cttgtttttg
     5401 cgcggcaagc atcacgggcg tgccgtgggt gtcgtcggcg cagcagtagt agcattcgtt
     5461 gccgcgcagt ttttggaagc gcacccaaac gtcggtttgg atgtgttcga ccatgtggcc
     5521 gaggtggatg ctgccgttgg catagggcag ggcggaggta actaagattt tgcgtgtcat
     5581 attgtgcttt gcaaacaatg ggtaaaggcg gattataccg caaatcaaac ggggaaatgc
     5641 cgtctgaagc ctgaaaaatc gggcttcaga cggcattttt gcaaaacggc gggagttatt
     5701 cgacggttac ggatttcgcc aggttgcgcg gcttgtccac atcggtaccg cgtgcgaggg
     5761 cggtgtggta ggcgaggagc tgcacgggga tagtatgcac gacgggggac agtttgccga
     5821 cgtggcgcgg tgcgcggata acgtgcacac ctttggtggc attaaaatta ctgtcgaggt
     5881 cggcaaagac gaaaagttcg ccgccgcgcg cgccgacttc ctgcatattg gctttgactt
     5941 tgtccaacag gctgtcgttg ggtgcgatga cgacgacggg catgttttcg tccaccaagg
     6001 caagcggccc gtgcttcagt tcgccggcag gataggcttc ggcgtggatg taggtgattt
     6061 ccttcagttt taacgcgcct tcgagggcaa tcgggtaatg gatgccgcgc cccaaaaaca
     6121 gcgcgctggt tttcttggca aactgttgcg cccaggcggc aatttgcggt tcgaggttta
     6181 aagcgtgctg cacgctgccg ggaagctggc ggagttcttc ggtgtaacgt gcttcgtctt
     6241 cttcggaaac caaaccgcgc actttcgcca gcgttaccgc caaaccgaac agcgcaacca
     6301 gctgcgtggt aaatgctttg gttgaggcga cgccgatttc cgcaccggca cgggtataaa
     6361 gcacgaggct gctttcgcgc ggcagggcgg attccatcac gttgcagatg gagaggctgt
     6421 ggcggtgtcc caaggatttt gcgtatttca acgcctccat cgtgtccagc gtttcgccgg
     6481 attgggaaat ggtaatgacc agttggtccg gatcggcaat cacgctgcgg tatcggtatt
     6541 cgctggcgat ttcgacatcg gccgggattt tggcgatgga ttccaaccaa tatttggcgg
     6601 tcagcgcggc gtaataggac gtgccgcagg caaggatttt gacgctgcgg atgctttcaa
     6661 acacgctttt ggcgtttttg ccgaagtttt cggggatgaa gccgccgtcg aggaaaacct
     6721 ccgccgtgtc tgcaatcgcg cgcggctgct catggatttc cttttgcata aagtggctgt
     6781 acggtcccag ttccaaagag gcgagcgaga gttcggatac cttgactttg cgttcggcag
     6841 gcaggccgct tttatcggtc agccgtttga tgccgtctga agccagcagc gcgatgtcgc
     6901 cgtcttcgag gtacgccacg cggcgcgtaa aggcgatgac ggcggatacg tccgaagcga
     6961 taaaggtttc atcgtcgccc aaagcgacca aaagcgggca gcccatacgc gccacaacta
     7021 attcatcagg cttgtcttgg gcaataaccg cgatggcgta tgcgccgtgg aaacgtttga
     7081 ccgcttcctg taccgcttca aacagcctgc cgccgttttg cgcgtattcg tgattgatgc
     7141 tgtgtgcgat gacttcggta tccgtttgcg attcaaaccg gtatcccaaa ccttccaagc
     7201 gtttgcgttc gctttcaaag ttttcgatga tgccgttgtg gacgacggct atcatcccac
     7261 cggaaatatg cggatgggca ttcggctcgg taacgccgcc gtgtgtcgcc caacgcgtat
     7321 gtccgatgcc gatgctgccc gctattcctt tttcacgggc agcgtcctcc ataagctgca
     7381 cgcgtccgac gcggcgcacg cgtttgattt tgccgtcggt gttgacggca atacctgatg
     7441 agtcataacc ccggtattcg aggcgtttga ggccgtcggt cagaaaatcg acgacgttgt
     7501 gatgggcgcg gatggcgccg acgataccgc acataactgt tccttagtat caggttgaaa
     7561 aaaaacaggt gcggacggct cccgtgccgc accttccgct tcggattata aaccgcctcc
     7621 cgcgccgaaa aacagcaaaa tgccgtctga aggcttgggc ttgctcaaaa aagggaggga
     7681 tttccctgtt tatccgagat gggcgttcag acggcattgc ctgccgccgg tttatagttt
     7741 ttacaaatca atattgacaa gctgaaaaaa aaaaacaata tactcgctcg gtcttaatgt
     7801 taaaggagta tggaaatgaa acaaatgctt ttagccgtcg gcgtggcggc ggtgttggcg
     7861 ggctgcggca aggatgccgg cggttacgag ggttattggc gcgaaaaatc ggacaaaaaa
     7921 gagggtatga ttgccgtcaa aaaagaaaaa ggcaattact tccttaataa aatcaacgtg
     7981 gttacaggta aggaagaatc tatgcttttg tctgaaaaag acggcgagct ttcgataaac
     8041 acggggatag gggaaatccc gatcaaactt tccgacgacg ggaaagagct gtatgtcgaa
     8101 cgcaggcagt atgtcaaaac cgatgcggcg atgaaggaca aaatcatcgc ccatcagaaa
     8161 aagtgcggac aaacagcaca ggcataccgc gacgcgcgaa atgcgttgcc gtcaaaccag
     8221 acgtatcagc agcatctggc ggcgatcgag caattgaaac ggcggtttga agccgagttt
     8281 gacgaattgg aaaaagaaat caaatgcaac ggcagaagcc cggcattgtt gctttagtag
     8341 gggacaaccg ggaggatgcc gccgtccgaa tcggatgtgc ggtttctgta ccggtacggg
     8401 cgggcaggaa tgtccgcctt ttttgttcgg atgcgtttga atacccgttt gattccgacc
     8461 gtttgcaagg ggtatttccg ttcgggcgga aattatagtg gattaacaaa aaccagtacg
     8521 gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctcaagc accaagtgaa
     8581 tcggttccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttga
     8641 tccactataa ttccgtcaaa taagaaagga attttgtgcc tgcggtatcg caaaacttcg
     8701 ccttaatgcg cccgattgcc tagggatggg cttcagatgg cattgttttc cggtttacgg
     8761 gcggtattcg ggcttcatac cgttgggtag gagctgccag acatatcccg tggttttctg
     8821 tttgccggca agttcgccgg cttcgtcgcc gtatccccaa aaataatcca cgcgcaccgc
     8881 gcctttaatc gcgctgccgg tatcctgcgc cataatcagg cggttgaggg ctttgcgggt
     8941 aaccggatgg gcggtggcga caaataaggg cgcacccaag gtaatgtagt gccggtcgac
     9001 tgcgccggca tattccccca tcagcggcgt gcccagtgcg ccgacagggc cgtcattgct
     9061 gcttccggca agctcgcgga aaaagatata gctggggttt tgacccaaaa cttcggcgag
     9121 gcgttgcgga ttttgccgca tataagactt aatgccctgc atggaggttt gtccgagttt
     9181 gaggtagccc ttatccgcca tatagcgtcc gatggaaacg tagggatgtt cgtttttgtc
     9241 ggcatagccg atgcggatgt atttgccgga cggggttttc agacggcccg agccttggat
     9301 gtgcataaaa aaaagttcga cagggtcttc ggcgtaaccg agtatcgggg ctttgccgtc
     9361 aagcgcgccg ccgttgattt ggttgcgcgt gtggtagggg aggaagcggc ttccttcaaa
     9421 cctgcctttg attgctgttg tgcgcgcggt gatggggaat cgggagaggt cggcggtatg
     9481 tgtgccgccg gtattgtcga ttgtgccgct gttttttccc gtctgcctga tgcggacaag
     9541 ggcttttccg ctccgcaaac cggcaggcag ggggacggag ataaaatcgt cgggaatacc
     9601 gtaaatcggg aagcgggctt gtgccgtccg cctgtcgtcg cccttcagca ccggttcgta
     9661 atagccggta accgtaccgg caaggcttcc gttgcctgca acctgccacg gcgtgaaata
     9721 gcgttcaaaa aactgttttg cctgaaagga atggacgggg gtttgaaagg cttgggcgca
     9781 cacatcctgc cagccttggc ggtttttcaa attggcgcag ccgaggcgga aggattgcag
     9841 gcttttggcg aaatcctgcg ccgcccagtg gggcagggac aggtgcggta caacggtata
     9901 gacggccccg ccgccgccga ccgtcgttcc ggcggggtcg gggatgccga ccggccggtc
     9961 cgggccgttg atgacggatg tgtcgggttg cggaaaggtt tggatgctct tgctttggca
    10021 ggcggcgagg atggcggcgg cgatgccgta cagggcggcg cggaataggt attttttcat
    10081 aatggacaat gttgccggca gtaataagaa agatggtttc gggcggcgtt gcggcagccg
    10141 tggagagggg attttaacac agggcgcagc tgcagcctgc ggaactttcc gccgcgcggt
    10201 actgcagata aaaataactt gcatttgtat ttacaagcaa tgaaaatatt ccgataatat
    10261 attattcatc atccttgttc gttcgcgttt atgctggtcg cttttttaat tatgttgcgc
    10321 gagggtattg aagccgcgct cattgtcggc atcgttgccg gttttctgaa acagtccgga
    10381 cattccaaac tgatgcctaa ggtctggttc ggggtcgtcc ttgcttcttt gatgtgtttg
    10441 gggctggggt acggcatcca ttcggcaacg ggcgagattc cccagaagca gcaggagttc
    10501 gtcgtcggca ttatcggttt ggttgccgtt gccatgctga cttatatgat tttatggatg
    10561 aaaaaggcgg cgcgttcgat gaagcggcag cttcaggatt ctgtgcaggc ggctttgaac
    10621 cgtggcagcg gtcaaggatg ggccttggtc ggtatggcgt ttcttgccgt ggcgcgcgaa
    10681 ggtctggaga gtgttttttt cctgcttgcc gtattcaaac agagcccgac gtggcagatg
    10741 ccggccggcg cggtagcggg ggttttggct gccgccgtga ttggcgcgtt gatttatcag
    10801 ggcgggatgc gcctgaatct ggcgaagttt tttcgttgga cgggggcgtt tttgattgcc
    10861 gttgccgccg gcctgcttgc cggctcgctg cgcgcgctgc atgaggcagg tatttggaac
    10921 gcgcttcagg atattgtgtt cgactcatca aaatatttgc acgaagacag cccgttgggc
    10981 gtgctgctcg gcggattttt cggctatacc gaccatccga cgcagggcga ggccttggtt
    11041 tggctgctgt accttattcc cgtcataact tggtttttgt gcggcagcag gccgtctgaa
    11101 actttaaccc gtaaagagga gctgaaatga gaaaattcaa tttgaccgca ttgtccgtga
    11161 tgcttgcctt aggtttgacc gcgtgccagc cgccggaggc ggagaaagct gcgccggccg
    11221 cgtccggtga gacccaatcc gccaacgagg gcggttcggt cagtatcgcc gtcaacgaca
    11281 atgcctgcga accgatggaa ctgaccgtgc cgagcggaca ggttgtgttc aatattaaaa
    11341 acaacagcgg ccgcaagctc gaatgggaaa tcctgaaagg cgtgatggtg gtggacgagc
    11401 gcgaaaacat cgcccccgga ctttccgata aaatgaccgt caccctgttg ccgggcgaat
    11461 acgaaatgac ttgcggtctt ttgaccaatc cgcgcggcaa gctggtggta accgacagcg
    11521 gctttaaaga caccgccaac gaagcggatt tggaaaaact gtcccaaccg ctcgccgact
    11581 ataaagccta cgttcaaggc gaggttaaag agctggtggc gaaaaccaaa acttttaccg
    11641 aagccgtcaa agcaggcgac attgaaaagg cgaaatccct gtttgccgac acccgcgtcc
    11701 attacgaacg catcgaaccg attgccgagc ttttcagcga actcgacccc gtcatcgatg
    11761 cgcgtgaaga cgacttcaaa gacggcgcga aagatgccgg atttaccggc tttcaccgta
    11821 tcgaatacgc cctttgggtg gaaaaagacg tgtccggcgt gaaggaaatt gcagcgaaac
    11881 tgatgaccga tgtcgaagcc ctgcaaaaag aaatcgacgc attggcgttt cctccgggca
    11941 aggtggtcgg cggcgcgtcc gaactgattg aagaagtggc gggcagtaaa atcagcggcg
    12001 aagaagaccg gtacagccac accgatttga gcgacttcca agccaatgtg gacggatcta
    12061 aaaaaatcgt cgatttgttc cgtccgttga tcgagaccaa aaacaaagcc ttgttggaaa
    12121 aaaccgatac caacttcaaa caggtcaacg aaattctggc gaaataccgg actaaagacg
    12181 gttttgaaac ctacgacaag ctgggcgaag ccgaccgcaa agcgttacag gcctctatta
    12241 acgcgcttgc cgaagacctt gcccaacttc gcggcatact cggcttgaaa taagccgcaa
    12301 gcgttcagac ggtatttggc ggcagatacc gtctgaagtt ttatagtgga ttaacaaaaa
    12361 ccagtacggc attgcctcgc cttgccttgc cttgccgtac tatttatact gtctgcggct
    12421 tcgtcgcctt gtcctgattt ttgttaatcc actatatccg ccatatattg cagggcggga
    12481 tttcaacctg ccgctatcgg ttaatggaaa aacggcgtgc agggataccc atcctgctgc
    12541 acggatattg aaggaaacac catgagcaaa aaccaacccg cacaaccgac caggcgcact
    12601 ctttttaaaa ccgcgatcgc agccggagca gtcggcgcaa tcggaggtta tctcggcggc
    12661 aaaaaacagg gcgaaaccgc cgaacgcacc gccgaaagcc aacactcgcc ccaagcctat
    12721 ccctgctacg gcgaacatca ggcaggcatc gttacgccgc agcaggcgtt ttcgattatg
    12781 tgcgccttcg acgtaaccgc gcaaagtgcc aagcagctgg aaaacctgtt ccgcacgctg
    12841 accgcccgca tcgagtttct cacccaaggc ggcgaatacc aagacggcga cgacaaactt
    12901 ccgccagccg gcagcggcat tttgggcaaa gccttcaacc ccgacgggtt gaccgttacc
    12961 gtaggggtgg gcagcagcct gtttgacggt cggttcggac tcaaagacaa aaaaccgatt
    13021 catttgcagg aaatgcgcga cttctccaac gataagctgc aaaaaagctg gtgcgacggc
    13081 gatttgagcc tgcaaatctg tgccttcacc cccgaaacct gccaagccgc cctgcgcgac
    13141 atcatcaaac acaccgtcca aaccgccgtt atccgttgga gtatcgacgg gtggcagccc
    13201 aaatccgaac ccggcgcgat ggcggcgcgc aacctgttgg gcttcaggga cggcacgggc
    13261 aaccccaaag tttccgaccc caaaactgcc gacgaggttt tgtggacggg ggtggccgcc
    13321 aacagcctcg acgaaccgga gtgggcgaaa aacggcagct atcaggcagt ccgccttatc
    13381 cgccactttg tcgagttttg ggacaggacg ccgcttcaag agcaaaccga cattttcggg
    13441 cggcgcaaat acagcggtgc gccgatggac ggcaaaaaag aagccgacca accggatttt
    13501 gccaaagacc ccgagggtga tatcacgccc aaagacagcc atatacgcct ggcgaatccg
    13561 cgcgatcccg aattcctcaa aaaacaccgc ctcttccgcc gcgcctacag ctattcgcgc
    13621 ggactcgcct caagcggaca gcttgatgtc gggctggtgt tcgtctgcta tcaggcaaac
    13681 cttgccgacg gattcatctt cgtgcaaaac ctcctcaacg gcgaaccgct ggaagaatac
    13741 atcagcccct tcggcggcgg ctatttcttc gtcttgcccg gcgtggaaaa aggcggcttt
    13801 ttggggcaag ggctgctggg cgtataaatc cgccatataa aaaacgccgt ctgaatccga
    13861 taaggggttc agacggcatt ttactgtttg ggcggtcagg cttttttgac gaattcagat
    13921 tttaaattca tcgcgctgcc gtcgattttg caggcgatgt tgtgatcgcc ttcctgcagg
    13981 cgtatgcctt tgactttcgt accttgtttg atcgtcatcg agctgccttt taccttgagg
    14041 tctttgatga ggatgatggt gtcgccgttt tgcagcaccg cgccgttggc atcgcgcact
    14101 tcttcggcgg cttcggctgc ggcggattcg gttttattcc attcgtgggc gcattcgggg
    14161 cagatgtatt gtccgccgtc ttcgtaggtg tattcggagg cgcattgcgg gcaagggggt
    14221 aatgacatgg tttgccgtcc ttatcggatg tttgttttgg ggtgccgtct gaaacctgaa
    14281 accggcttca gacggcatag ctttattgtt tgtctttttc aggacgcacc cagccttcga
    14341 tgacggtttg gcgggcgcgg gcgagggcga gtttgttgtc ttcgacattg cgggtaatcg
    14401 tgctgcctgc gcctgtggtt actttgttgc ccagtgttac gggggcaacc agtacgcagt
    14461 ttgaaccgat gcggacttcg tcgccgatga cggttttgtg tttgtgcacg ccgtcgtagt
    14521 tggcaataat cgtgcctgcg ccgaagttgg ttttgctgcc gacttcggca tcaccaatgt
    14581 aggtgaggtg gttggctttg gtgccgttgc cgatggcggc gtttttgact tcgacgaagt
    14641 tgccgatgtg tacgtcggcg gctagtttgg cttgcggacg caaacgggcg taggggccga
    14701 ttcggttgtt ttcgccgact tcgcagtctt cgaggtggga gaagggggag attttgctgt
    14761 ttgcgccgat tttggcgttt ttgatgacgc agtttgcgcc gatttcgacg ttgtcgccga
    14821 gctcgatttc gccttcaaag atacagttcg catcaattat gacatcttgt ccgtgtttca
    14881 gacggccgcg taaatcgaaa cgtgccggat cgcgcagggt tacgcctgct ttgagcaatt
    14941 cttgtgcctg ttcggtttgg aagatgcgtt cgagttcggc gagctggcat ttgttgttca
    15001 cgccggcggc gaggtaggag gcgcgcactt ggacgggatg gactttgatg ccgtcggcaa
    15061 cggctttggc gatgaggtcg gttaaataat attcgccttg ggcattgttg ctggaaaggc
    15121 tgttcaacca agcttcgagt ttggcgttag gcagaaccaa aatgccggta ttgatttctg
    15181 tcacggcttt ttgggcgacg tttgcgtctt tttcttcgac aatggcggtt acgctgccgt
    15241 tgccgtcgcg gatgatacgc cccaagcctg tcgggtcggt gggaacatca gtcaacagcc
    15301 cgactttgtc gcctgcggct tcgagcaggg ttttgagggt ttcaacgtca attaaaggaa
    15361 cgtcgccgta cagcaccagc gtgcggcctt cggcggcaag gtggggcagg gcggttttga
    15421 cggcgtggcc ggtaccgagc tgttcggttt gttcgaccca gacgacatcg cgtttgacgg
    15481 tgtccaaaac ttggtctttg ccgtgtccga cgacgacgca gatgttttgc ggattcagcg
    15541 cggcggcggt atcgataacg cgcccgagca ttgtttcgcc gccgatttcg tgcaaaacct
    15601 tgggcatttt ggaatacatg cgcgtgcctt tgccggcggc gaggataacg atgtttaaag
    15661 tgttgtgcgg catagcgttt tcctgtgcaa tgccgtctga aagggcttca gacggcatag
    15721 ggtaggttta tcggttttga aatttgggct tttgccagtt ctgtccgcgt acgccttgtt
    15781 cggaatcgtc ggtttggtcg ggcagcacgg catggcgttc gggacggtat tggttgtagt
    15841 tcatattttt cgagtagctg ccgtcttggt aataaacggg cgtgccgacg ggatattttt
    15901 gacggacggc ggttttgccg ttgccgtctt gataagtttc ccacgcgcag cccgacaaaa
    15961 gggcggcggc agcgatcagg aagaggaagg ttttacgcat ggcttttctt tcgtattttc
    16021 gggggttagg gggtattgta atgattttgg cggcgttctg acaaagtttc tgcataccga
    16081 gccagttgcg ccatatcgcg tgcgcaggtg tcgataaagg gcagcgcgtg ggattttgca
    16141 ccgaaccgga cggttttcat gcccaatgct tttgcctgat gcaggttgtc cgcgctgtcg
    16201 tccaccataa tgcagcattc gggcggtacg tccaaaaggc ggcagacatt gagatacgct
    16261 tgcggattgg gtttgtacag cagtccgaaa tcatccgtgc cgaacagtgc gtcgaaacgg
    16321 ttttccaaac cgagtgcgcc gacaacggca cggacgtaaa acgacgggcc gttggaaaaa
    16381 accgccttgc gcccttttag gcggctcagg gtgttttgtg tttcaggcat gccgtgcagc
    16441 ctggtcagga ttgcatcgat cggatggctt ttgcgcaaaa attcggcgat gtcgatttcg
    16501 ggatggtgga tttgcagtcc ggcgagcgtt gcgccgtagc ggtgccaata gtcttgacgc
    16561 aggtcggacg cggcagattc ggagagtttg aggcggcgtg ccatatagcg tgttatagcg
    16621 cggttgatga gtgtgaagat gccggcatcg gcatcgtgca gcgtgttgtc gaggtcgaac
    16681 agccacacgg tcgggttttc ttgcatgttg aaccgtgaaa atttgttaga atgttatttt
    16741 acagcgaata gaggaggact cggaatgaaa cggaaaattt ggctgctgcc gctgctggcg
    16801 gtttcggcat acctgcaggc gcagacggaa gtcaggctgg cggtgcataa gtcgttcagc
    16861 ctgcccaaag ggctgattgc gcgcttcgag cgggcaaacg atgcgaaggt gtcgattatt
    16921 caggcgggcg gcgcgaacga aatgctcaac aaactgattt tgagccgcgc caacccgatt
    16981 gccgacgcgg tgtatggttt ggacaacgcc aatatcggca aggcgcggga aatgggcatt
    17041 ttggcggcgg cgcaacccga atccgccccc gtcgcggtcg ggctgccttc ggctttggcg
    17101 gtcgattacg gctatgtgtc catcaattac gacaaaaaat ggtttgaagg caaaaagctg
    17161 cccctgccgc aaaccctgca ggatttgacc cgccccgaat ataaaaacct attggtcgtg
    17221 ccgtcccccg ccacgtcgtc cccggggctg ggcttcctga tggcgaacat cagcggtctg
    17281 ggcgaagaag gcgcgttcaa atggtgggca cagatgcggc agaacggcgt gaaggtcgcc
    17341 aaaggctgga gcgaggcgta ttacaccgac ttttcgcaca acggcggcgc gtatccgctg
    17401 gtggtcggtt atgccgccag cccggcggcg gaagtgtatt tttccaaagg caaatacagc
    17461 gagccgccga cgggcaacct gtttttaaaa ggcggcgtat tccgccaggt cgaaggcgcg
    17521 gcggtcttga agggcgcgaa acagccgaag ctggcggcaa aactggtgca atggctgcaa
    17581 agtcgggaag tgcagcaggc ggttccgtcc gaaatgtggg tttaccccgc cgtcaaaaac
    17641 acgcgcctgc ccgacgtgtt ccgcttcgcc caagccccga cgcacaccac cgcccccgcg
    17701 cagcgcgata ttgatgcgaa ccagcgcgga tgggtttccc gttggattag aacggttttg
    17761 aaataaaaca aacataccgc cccgcagggc ttcatacggc atttttacac ctgtgccgat
    17821 tacgccgcac ggggcggatg ttcgatcaag aggaaaacaa tggacttcaa acaatttgat
    17881 tttttacacc tgatcagtgt ttccggttgg gagcatctgg ctgaaaaggc gtgggcgttc
    17941 gggctgaacc ttgccgccgc gctgcttatt tttttggtcg gaaaatgggc ggcgaaacgc
    18001 attgtcgctg tgatgagggc ggcgatgacg cgcgcgcagg tcgatgccac gctgattagt
    18061 tttttgtgta atgttgccaa tatcggctta ttgattttgg tgattattgc cgcattgggc
    18121 agattgggcg tttccacaac atccgtaacc gccttaatcg gcggcgcggg tttggcggtg
    18181 gcgttgtccc tgaaagacca gctgtccaat tttgccgccg gcgcgctgat tatcctgttc
    18241 cgcccgttca aagtcggcga ttttatccgc gtcggcggtt ttgaaggata tgtccgagag
    18301 attaaaatgg tgcagacttc tttgcggacg accgacaacg aagaagtcgt gctgcccaac
    18361 agcgtggtga tgggcaacag catcgtcaac cgttccacac tgccgctgtg ccgcgcccaa
    18421 gtgatagtcg gcgtcgatta caactgcgat ttgaaagtgg cgaaagaggc ggtgttgaaa
    18481 gccgccgtcg aacacccctt gagcgttcaa aacgaagagc ggcaggccgc cgcctacatc
    18541 accgccttgg gcgacaatgc catcgaaatc acattatggg cttgggcaaa cgaagcagac
    18601 cgctggacgc tgcaatgcga cttgaacgaa caagtggtcg aaaacctccg caaagtcaat
    18661 atcaacatcc cgttcccgca acgcgacata cacatcatca attcttaaac gccgtctgaa
    18721 aggggagtgg gaaatggacg cgctgcacac catcgcccga aacctgacga aaaaacgtca
    18781 aaccgtaagc tgtgccgaat cctgtacggg cggaatgctt gccgccgcat tcacaagcgt
    18841 tgcaggcagt tcgcaatggt tcgatcaaag ttttgtaaca tacagcaaca aagccaaaga
    18901 agaccgcttg ggcgtgttgc ccgaaaccct gctcgaacac ggcgcggtca gccgccaaac
    18961 cgtctatgag atggcgcgcg gcgcgaaagc cgtggcgcag gcggattacg ccgtcggtat
    19021 ttccggcatc gccggaccgg gcggcggcag cgaaagcaaa cccgtcggca cagtttggtt
    19081 cgggtttgcc tttccgggcg gaagttgcga agcaatgcgc cgttttgatg gcaaccgcga
    19141 atccgtccgc gcgcaggcgg tcgccttcgc gttggaacgg ttggcggggc tgattgaaaa
    19201 cggcggcgat gccgtctaaa caaaatctcc gtctggacaa aatccccgcc ggataaaaaa
    19261 taccgtctga aacgttgcag gtttcagacg gtattttgtc gggttaggcg gcggtgcggc
    19321 ttatttcact ttactcttca acgcgccgta gcctgccgcg tccatttgtt ccagtgggat
    19381 gaatttcaag ctcgcgccgt tgatgcagta gcgcagtccg cctttgtcgc gcgggccgtc
    19441 ggggaagacg tgtcccaaat gcgagtcggc ggcgtggctg cgcacttcgg tgcggcgcat
    19501 gttgtagctg aaatcatcgt gttcggtaac ggattttgca tcaatcgggc gcgtgaagct
    19561 cggccagccg cagccggaat catatttgtc ggcggagctg aacaaaggtt cgccgctgac
    19621 aacgtccaca taaatgccgg gtttgaacaa atggtcgtat tcgtggctga aggcatattc
    19681 ggtcgcgctg ttttgggtca cttggtactg ctcttcggtc agggtgcgtt tgagttcggc
    19741 gtcactcggt tttttatacg ttgccgcatc gaagcctttg ccttgcgggg cggtcttggt
    19801 tttgccctgc agcggttcgt cggctttgcg gatatcgatg tggcagtagc cgttggggtt
    19861 tttaatcaag tagtcctgat ggtattcctc ggcatcgtag aagtttttca gcggctcgtt
    19921 ttcaacaacg aggggcagtt ggtatttttg ctgctcgcgt ttgagggcgg cggcgatgac
    19981 ggctttttcg gcggggtcgg tgtagtacac gccgctgcgg tattgcgtac cggtgtcgtt
    20041 gccctgtttg ttgaggctgg tcggatcaac gacgcggaag aaatattgca ggatgtcgtc
    20101 taggctgagt ttgtcggcat cgtaggtcac tttaacggtt tcggcgtggc ccgtatggcg
    20161 gtaggacacg tcttcatagc tcggattttt cgtgttgccg ttggcgtagc cggataccgc
    20221 gtcaaccacg ccgtcgatgc gttggaaata ggcttccaag ccccagaagc agccgccggc
    20281 gaggtaaatg gtgcgcgtgt tcatgatttt tgaatccttt ttctgagtgt cgggtttgta
    20341 gaacgaatgt ttcaagctgc ccaaatcggc attcgggtcg cggattaacg ccaacgcctg
    20401 cgcttcgttg atgctgcctt tgacgatgcg ctgcacgtcg ccgtctttac cgattaacgc
    20461 ccacgagggg taaacgctga tattcaggct ttgggcgatc gtgccgccgt tgtcggttac
    20521 gacgggcagc ttgggataat tcaaaccggc ataccatttt tggaagtcgc cgtctttttt
    20581 ctcgtgcaaa aagcccggag acgcgacggt aatcaggttg gcggagctga attttgcatc
    20641 ttgcgcccat ttttcggtct gccccaattc ggacaaacac aaaggacacc agctcgccca
    20701 aaatttaatc agcgtcggtt tgtctttttt caagtaaaca ctggcggggc ggttgtctgc
    20761 ggttttcaaa gtggataaag tgtgcggcac ggtcgcggct ccggcatcga cgattttggg
    20821 cgaacaagca cccagcgcaa gcaggcagcc gaacttggcg caaagggaaa agaaagtacg
    20881 gtgtttcatt ttaatgtttc ctgtgtggac ggtttgcatg attagacgtt tgagatgccg
    20941 aaaccttaca gctcggattt tcagacaacc ttgccgcgta aaatacgcta caatacgccc
    21001 tgtttcaagt ttctaaaatt aaaaggaaaa ttcaatgttc agcttcttcc gtcgcaagaa
    21061 aaaacaggaa acgccggctc ccgaggaggc tcaagttcag gaaactgcag caaaagcaga
    21121 atccgaagtt actcaaatag ttgaaaatat taaagaagaa gtcgaatctt taacggaaag
    21181 cgtcaaaggg caggtcgaat cggctgttga aaccgtcagc ggtgcggttg aacaagtaaa
    21241 ggaagccgtt gccgagatgc tgtctgaagc agaggaagcg gcggaaaaag cagcggaaca
    21301 agtcgaagcg gcaaaagaag ccattgccga aaccgtcggc gaggctgtcg ggcaagttca
    21361 agaagccgtt gcgacaactg aagaacacaa gctcggttgg gcggcgcgtt tgaaacaagg
    21421 cctgaccaaa tcgcgcgaca aaatggcgaa atcgctggcg ggcgtgttcg gcggcggaca
    21481 aatcgacgaa gatttgtacg aagagctgga aaccgtgcta attaccagcg acatgggcat
    21541 ggaagccacc gaatacctga tgaaagacgt gcgcgaccgc gtcagcctca aagggctgaa
    21601 agacggcaac gaattgcgcg gcgcgttgaa agaagccttg tacgacctga ttaagcctct
    21661 ggaaaaacca ttggtcttgc ccgaaaccaa agagccgttt gtcatcatgc ttgccggcat
    21721 caacggcgcg ggcaaaacca cgtctatcgg caaactcgcc aaatatttcc aagcgcaggg
    21781 caaatccgta ttgctggcgg caggcgacac tttccgcgcc gccgcccgtg agcagcttca
    21841 ggcatggggc gaacgcaaca acgttaccgt gatttcgcaa accacgggcg attccgccgc
    21901 cgtgtgcttc gatgccgtcc aagccgccaa agcgcgcggc atcgacattg tgctggccga
    21961 caccgccggc cgcctgccca cgcagcttca tttgatggaa gaaatcaaaa aagtgaaacg
    22021 cgtgctgcaa aaagccatgc ccgacgcgcc gcacgaaatc atcgtcgtgc ttgatgccaa
    22081 catcgggcaa aacgccgtca accaagtcaa agcctttgac gacgcattag ggctgaccgg
    22141 tttaatcgtg accaaactcg acggcacggc aaaaggcggc atcctcgccg cgcttgcctc
    22201 cgaccgccca gttcccgtcc gctacatcgg cgtgggcgaa ggcatagacg atttgcgccc
    22261 gtttgacgcg cgcgcgtttg tggacgcact gctggattga gccgaaatgc cgtccgaaaa
    22321 cagcagaccg atgccgtcat tcccgcgcag gcgggaatct agaccattgg acagcggcaa
    22381 tattcaaaga ttatctgaaa gtccgagatt ctagattccc actttcgcgg gaatgacgaa
    22441 gagttgcggg aatgatggaa agctatggga ataacgaaag gttaaagtaa tcacgggatg
    22501 gtgttcgcgg gaatataaat ttaaataatt caaaagggta ttatatgcag cctgcggttt
    22561 atattttagc aagccaacgt aatggcacgt tatacattgg cgttacatct gatttggtgc
    22621 aacgtattta ccaacatagg gagcatttga ttgagggatt tacatcacgg tacaacgtta
    22681 ctatgctggt ttggtatgaa ctgcatccta cgatggagag cgcaattact cgggaaaaac
    22741 agttgaagaa atggaacagg gcttggaaat tgcaactgat tgaagaaaat aatgtttctt
    22801 ggcaggattt atggtttgat attatttagc ccgtcattcc tgcgca
//
LOCUS       DAXHMX010000013        22499 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHMX010000013 DAXHMX010000000
VERSION     DAXHMX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22499
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.013"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(157..996)
                     /gene="htpX"
                     /locus_tag="ACFM4S_000389"
     CDS             complement(157..996)
                     /gene="htpX"
                     /locus_tag="ACFM4S_000389"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6050138.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            1211..1858
                     /gene="adk"
                     /locus_tag="ACFM4S_000390"
     CDS             1211..1858
                     /gene="adk"
                     /locus_tag="ACFM4S_000390"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6050139.1"
                     /translation="MKALFLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            2384..3124
                     /gene="pyrF"
                     /locus_tag="ACFM4S_000391"
     CDS             2384..3124
                     /gene="pyrF"
                     /locus_tag="ACFM4S_000391"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6050140.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            3179..4150
                     /gene="hldA"
                     /locus_tag="ACFM4S_000392"
     CDS             3179..4150
                     /gene="hldA"
                     /locus_tag="ACFM4S_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6050141.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            4187..5422
                     /locus_tag="ACFM4S_000393"
     CDS             4187..5422
                     /locus_tag="ACFM4S_000393"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6050142.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFSRVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            5424..6542
                     /locus_tag="ACFM4S_000394"
     CDS             5424..6542
                     /locus_tag="ACFM4S_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6050143.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEVLLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            6583..7587
                     /gene="rfaD"
                     /locus_tag="ACFM4S_000395"
     CDS             6583..7587
                     /gene="rfaD"
                     /locus_tag="ACFM4S_000395"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6050144.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LNWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVTFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            7660..9204
                     /locus_tag="ACFM4S_000396"
     CDS             7660..9204
                     /locus_tag="ACFM4S_000396"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6050145.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPSQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGSFFKKETNNNVLTEEHIADIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            9321..9404
                     /locus_tag="ACFM4S_000397"
                     /pseudo
     CDS             9321..9404
                     /locus_tag="ACFM4S_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible D domain protein"
     gene            9535..9876
                     /locus_tag="ACFM4S_000398"
     CDS             9535..9876
                     /locus_tag="ACFM4S_000398"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6050146.1"
                     /translation="MDIQSKAKKLIEMIQTAPVEWKPLGEVASIGKGKQLNKSLLREK
                     DKYPVINGGINPSGYWHEYNNEAGTIVISQGGASAGFVNWMDIPFFAGAHAYTIKANE
                     DKLLQRFAYYF"
     gene            9879..10118
                     /locus_tag="ACFM4S_000399"
     CDS             9879..10118
                     /locus_tag="ACFM4S_000399"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6050147.1"
                     /translation="MKQITFMQCQYGAGIPALAKSELQNLLIPIPPLETQQKIVKILD
                     KFTELEAELALRKRQYRYYRDFLLDFDNQIGGGDS"
     gene            10087..10704
                     /locus_tag="ACFM4S_000400"
     CDS             10087..10704
                     /locus_tag="ACFM4S_000400"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6050148.1"
                     /translation="MTIKSGGGIADGYQCRLKDVVWKTLGEIAEYSKNRICSDKLNEH
                     NYVGVDNLLQNREGKKLSGYVPSEGKMTEYIANDILIGNIRPYLKKIWQADCTGGTNG
                     DVLVIRVTDEKVNPKYLYQVLADDKFFAFNMKHAKGAKMPRGSKAAIMQYKIPIPPLP
                     EQEKIVAILDKFDTLTHSVSEGLPYEIALRRKQYEYYREQLLAFR"
     gene            10691..10912
                     /locus_tag="ACFM4S_000401"
     CDS             10691..10912
                     /locus_tag="ACFM4S_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050149.1"
                     /translation="MPFGRVGFSPPKQQQAATTVKQRQAGRWAEAHPTASGNRPAFRH
                     SVWKPENMAADAQNKEKKPIEMIQTASVG"
     gene            11096..11208
                     /locus_tag="ACFM4S_000402"
                     /pseudo
     CDS             11096..11208
                     /locus_tag="ACFM4S_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            11239..14370
                     /locus_tag="ACFM4S_000403"
     CDS             11239..14370
                     /locus_tag="ACFM4S_000403"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC6050150.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSTDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFHDHIVDGKLI
                     MKGNALGSESTESVFGRQLHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKK
                     LSAAENRQALLHPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYE
                     AFKTQQAGSLHPLKVATIFSFAANEEQNAVGEIADETFEPEAMDSSAKEFLQAAINDY
                     NACFKTNFGTDSKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNL
                     RYHGLMQAFSRTNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEE
                     YMNGYIDSQTGEARRGYLDVAKELRERFPDPDEIETEKDKKDFAKLFGEYLRAENVLQ
                     NYDEFAALRELQNVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYN
                     DIRDWLSRQKAGEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAE
                     LVEEIRRIIRASIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAG
                     LIAAEDLNETAAKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIA
                     AFVEKFAGIGTDI"
     gene            complement(14410..16701)
                     /gene="clpA"
                     /locus_tag="ACFM4S_000404"
     CDS             complement(14410..16701)
                     /gene="clpA"
                     /locus_tag="ACFM4S_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6050151.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(16703..17005)
                     /gene="clpS"
                     /locus_tag="ACFM4S_000405"
     CDS             complement(16703..17005)
                     /gene="clpS"
                     /locus_tag="ACFM4S_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6050152.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            17248..17451
                     /locus_tag="ACFM4S_000406"
     CDS             17248..17451
                     /locus_tag="ACFM4S_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6050153.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            complement(17748..19079)
                     /gene="pmbA"
                     /locus_tag="ACFM4S_000407"
     CDS             complement(17748..19079)
                     /gene="pmbA"
                     /locus_tag="ACFM4S_000407"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6050154.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARYTAEDDCA
                     GLADASLMAQHIGDPDLYHEWDLDTEAAVELAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHQQGTRHSISCSVVAADENGMQRDYWYDSSCRHQDMDSPTQIGQT
                     AAERTLRRLNSRSIPTGSYPVLFDATVSGGLIGHLVGALSGGSLYRQSSFLIDSIGKQ
                     VLPDFLSLREEPHIPRAFGSTYFDSEGVATRPRFVIRDGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            19234..19740
                     /gene="yjgA"
                     /locus_tag="ACFM4S_000408"
     CDS             19234..19740
                     /gene="yjgA"
                     /locus_tag="ACFM4S_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6050155.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAVHNAFLQRVE
                     QARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            19787..20506
                     /locus_tag="ACFM4S_000409"
     CDS             19787..20506
                     /locus_tag="ACFM4S_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050156.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFANILYTPPPFLILDA
                     AGAVLALPVAALIAATNSSDK"
     gene            20575..22275
                     /gene="recJ"
                     /locus_tag="ACFM4S_000410"
     CDS             20575..22275
                     /gene="recJ"
                     /locus_tag="ACFM4S_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6050157.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDVEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTSFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
BASE COUNT         5949 a         5471 c         5810 g         5269 t
ORIGIN      
        1 ctgtccggac acggtttatc agggtatatc aatgcggcgt atccggtgcg gaaatggata
       61 cggttggtgt tggtatggaa acctgatgtt ttcagacagc atatacaaaa aaccgtactg
      121 cttgcgcgta cggttttttt gttgtttcaa atcggctcag agcgatttga ggcgggcgat
      181 tcggttgtcc agcgaagggt gggtgctgag cagggagtcg cgcgtatctc cggcgatgcc
      241 cattgcgttc atttcttcgg gcaaatcgac cgggttgcct ttaagccttt gcagggcgga
      301 aatcattttc ggtgcgccga ccagttttgc cgcgcccgcg tcggcgcggt attcgcgttg
      361 tcggctgaac cacatgacaa ttaagctggc aaggaagccg aataggattt ggaataccat
      421 gctgaccagg aaataagttc cctgcgactg gctgccgtcg ttgtttcggg caatcaggtt
      481 ggcaataatg cgcgacagga acacgacgaa ggtattgacc acgccttgaa tcagcgtcag
      541 cgtaaccata tcgccgttgc cgacgtgtgc catttcgtgc gccaatacgg cttccacttc
      601 gtcgcgcgtc atatggtcga gcaaaccggt gctgacggcg atcagggagc tgtttctcga
      661 tgcgcccgtg gcaaaggcat tgggttcggg ggagtggtag atggcgactt cgggcgtttt
      721 caggttccat tgccgcgctt gggcttcgac agtgttcaaa agccaggctt cttcttcggt
      781 gcgcggcgtg tcgataactt ccgcgccgac cgattgtttg gcgatgaatt tggacatcag
      841 cagcgaaata atcgaaccag tgaagccgac gacggcggaa tacgccaaca ggctgcccgt
      901 gccgccccgg ctgttgatgc ccaaaaccgc caaaacaatg ttgattacga ccaaaacagc
      961 gatattggta gccaaaaaca gaaaaattcg tttcacggat gttccttttt ggtagggtgt
     1021 gatgttttga aattttgggg attgtcccaa aaagttgccg gcttgtgaat atcagactcg
     1081 gcaaaggtat gcaaaacatt tgcttgcaaa tggcagtttg tgcagttggt ttttgaacta
     1141 ttgtgccaag ccgtgtagaa tcgtaaacca tctgtttgat tccaataaac acatttcaaa
     1201 ggatcacttc atgaaagcat tatttttagg cgcgccgggc gcgggcaaag gcactcaggc
     1261 gcaattcatc accgccgcgt tcggcattcc gcaaatctct accggcgaca tgctccgcgc
     1321 cgcgattaag gcaggcacgc ccttgggttt ggaagcgaaa aaaatcattg atgaaggcgg
     1381 cttggtgcgc gacgacatca ttatcggcat ggtcaaagaa cgcatcgcgc aagacgactg
     1441 caaaaacggt ttcctgtttg acggtttccc gcgcacattg gcacaagccg aagcgatggt
     1501 tgaagcaggc gtggatttgg atgcagtcgt tgaaatcgat gtgcctgaca gcgtgattgt
     1561 cgaccgcatg agcggccgcc gcgtgcattt ggcttccggc cgtacttacc acgttaccta
     1621 caacccgccc aaagttgaag gcaaagacga cgtaaccggc gaagatttga ttcagcgcga
     1681 cgacgacaaa gaagaaaccg tgaaaaaacg ccttgccgtt taccacgagc aaaccgaagt
     1741 tttggtcgat ttttacagca aactggaagg cgaacacgcg cctaaataca tcaaagttga
     1801 cggcactcag ccggtagaag ccgtgaaagc cgaagtattg ggcgcattgg gcaaataaat
     1861 cgaaaaggtc gtacccacgg gcaggcttcg cactctgaaa acagaaaatc aggttttcag
     1921 acgacctgtt tttgataaac agcgtgttgc aaccgaaaaa taatcatttg gcgtcattcc
     1981 cgcgcaggcg ggaatccatt tctgaatttg ggcaatcgct gtttaaatct gatgaactga
     2041 gttttatcaa tggattcccg cctacgcggg aatgacggct gatgtaccgg ttcaaattta
     2101 tccgaaacag tttgtcggag gcttgagtcc gcgtaggtcg gacatcaatg cccgacctac
     2161 ggtttgaatt tacgttctta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
     2221 aggcagtaca aacagtacgg aaccgattca ctttgtgctt cagcacctta gagaagcgtt
     2281 ctctttgaac taaggcgaga caacgccgta ccggtttaaa gttaatccac tatactgcga
     2341 aaaagacgat aaaggtcgtc tgaaaacccg aaacgaaaac accatgaatc ctttaatctc
     2401 cgacttccaa actccgcaac aacgcacccc cgttatcgtc gcccttgatt tttccaacga
     2461 aaaagacacg ctcggattcg tccgcaacct tgacccgaca ttgtgtcaaa tcaaaatcgg
     2521 caaagagctg tttaccgcga cgggacgcaa tttggcagaa agcttaatca atcaaggttt
     2581 caaacttttc ctcgatttga aataccacga tattccccac accgtcgcgc aagcctgcaa
     2641 agtcgctgcc gatatgggcg tttggatggt cgatatgcac gcatcgggcg gccgccgtat
     2701 gatggaagcc gcagcagaag ccgttgccgg atacggcacg aagccgctct taatcggcgt
     2761 aaccgtgttg accagcatgg aacaaagcga tttggcggaa atcggtttga acaccgcccc
     2821 tgaagaacaa gtcatccgct tggcgaaact ggcgcaaagt tcgggcttgg acggcgtggt
     2881 ctgttccgcc caagaagccg caccgctgcg ccgcgagttg ggacaagatt ttgtcttggt
     2941 cacgcccggc atccgcttgg acgttgccgg caataatgat gaccagcgcc gcatcatgac
     3001 accggccgaa gccttggctg ccgcttcgac ttatttggta atgggtcgtc ctgtaaccca
     3061 agctgccgat ccggtagccg tattgcgcga agtgaaccgc gtggcaaacc ttgaagcaaa
     3121 ctgattttca gacggcctta caggctgagg ccgtctgaaa aaatacaacg gaggcaatat
     3181 gtccgccaag ttccaacaag aaaccctcaa atcccgtttc gcgcaagcca aagtcctggt
     3241 tgtcggcgac gtgatgctcg accgctattg gttcggcgat gtgtcccgta tttcgcccga
     3301 agcccccgtg ccggtggcga aaatcggacg aatcgaccaa cgcgcgggcg gagcggcaaa
     3361 tgtcgcgcgc aacatcgctt cgttgggcgg cagggcaggg ctgttgtccg taaccggcaa
     3421 cgacgaagcc gccgacgcgc tcgatgcgct gatggtgcag gacggcgtcg cctcctatct
     3481 gatgcgcgac aaacaaatcg ccaccaccgt caaactgcgc gtcgtcgccc gcaaccagca
     3541 gcttatccgt cttgattttg aagaacatcc caactgcgaa gtgttggaac aaatcaagca
     3601 gaaataccgc gaaatcttgc ccgaatacga cgcaatcatt ttttcagact acggcaaagg
     3661 cggcctgtcg catatctccg atatgatcga ttgggcgaaa cacgccggca aaaccgtctt
     3721 aatcgacccc aaaggcgacg attacgaaaa atatgtcggt gcaactctga ttacgcctaa
     3781 ccgcgccgaa ttgaaagaag tggtcggcag ttggaaaaac gaaagcgagc tgaccgaaaa
     3841 agcgcaaaac ctgcgccgcc acctcgacct gaccgccgtt ttactgaccc gaagcgaaga
     3901 aggcatgacc ttgttcagcg aaggcgaacc gatttaccag cccacccgcg cccaagaagt
     3961 ttacgacgta tccggtgcgg gcgacaccgt cattgccgga atgggcttgg gtttggcggc
     4021 aggctgcacc atgcccgaag ccatgtacct tgccaatact gcggccgggg ttgtcgtggc
     4081 gaaactcggt acggcggttt gctcgtttgc cgaattgatc aaggcattgt cagggcaatc
     4141 aacaatgtag ttttcatatt gataagataa acagaacgat ataagtatga ctatttcgac
     4201 aatggcacag acacacgata ctcgattaca aaaaactttg ctctttccca gtcaacaagg
     4261 ttggaaatct aaaacttttc ttaaacctga ttcacatatt agattagcaa ccgtattcag
     4321 cgggattggt gcggttgaac aggcattcca ccgattaaat ttaaaccata ccattgtttt
     4381 tgcaggagat attgatccat acgttaaaaa aagttatctt ggaaactata aattaaatga
     4441 agatttttgg cataacgaca ttactcaatt tgatgcgaga aagtttagaa atcaagttga
     4501 tattttagtt ggaggcagtc cttgccaagc attttccatg gttggcaaac gtgcaggatt
     4561 agaagataca cgaggaacat tattctatga attttctcgc gttgtggatg aagtccaacc
     4621 aaagatattt atttatgaaa atgtaaaggg cttgctcaat catgataatg gaaaaacttg
     4681 gaaagttgta aaaagtgttt tttattcact tggttatgac ttatatttcc aaataatgaa
     4741 tagtaaggat tatgggattc ctcaacatcg tgagcgtatt tttgttgttg gctttcatac
     4801 ccctcctata aatggttttc agtttcctga aaagattgaa ttagaacata ctatgcaaga
     4861 ttttttggag gactatactg atagcaaata ttttttacgt gaaaagggtg tgaaatttgt
     4921 taccagttct aaaaatagac aaaaacgtta tacacagatt aatggagaaa ttgccttatg
     4981 ccaaaaagca aatcaacaat ttaattggca tggtgatttt atttttcagg cagcccgcga
     5041 atctgaattt gatgacttta tttttgatgt aaataacgtt gaggaaaaat attatctttc
     5101 tgaaaaaatc aaaaattatg ttttggcagg aggaacaaaa aattttaaaa ccagtacgga
     5161 aactgatttg cctgtagctc gcccattatt gcaaactatg cataaaatgc atcgtgccgg
     5221 ggttgataat tatgttactc ataatcgtgg acgtattcgt aaattaacac ctagggaatg
     5281 tttgcggcta atgggtttta gagatgattt taaaatttta gttagtgata ctcaaatgta
     5341 tcgccaagcg ggaaacagta ttgttgttga tgtattaatc gctatattaa aacaaatgga
     5401 tattacgctt tatggagagt aaaatgccgg attttcaaag aattactatt gaaaacatag
     5461 agtattttat tgtcgatagt attcaaaatt tacgtgcgga agatagtttt attcacagga
     5521 ataacaagtt atctgttttt ggtggtaatg gggaagcacg caaatatgta ggtagttata
     5581 ttgatgatag tggtcgaaaa ctaagtacat tttttgagta tgaaaattgg gggattactg
     5641 agacaaaaaa tggaaggagg gtagaatatc ctctcattca gaaaaattgt ttttttaata
     5701 agaaaaattt acagcgttat ttggttgatg caaaagttga atatcacaaa caagaacaga
     5761 aatatcatca tgatatttca aggttttatg atgattacgt tttaagtatt aataatctgc
     5821 ctacagaaaa tattttcttt tcaatccatg atgtatcaga ccatttggaa aattctaaag
     5881 gttaccgtag gggttatatc cgttcggagg atgatatatg gtcaatttgg cgtaagattg
     5941 tattgcccaa aattagttat ttatcaattc ttaaactatt gcctgtcaaa gatatagaag
     6001 attcagaacc attattttat ttccgaatat ttttggatta tcaatttcgc tctatcgtgc
     6061 acccgcaact cttatcaaga gaaaaattag aaataccggc ttcagaattt aatcaattcg
     6121 tagagcaaga aatcattaaa cagaaaagta gaaaagggca gcagaaatat cgcaaggatg
     6181 ttataaatca tatgccgcaa tgtccattta cattaattac agatgaggtt ttgttaaggg
     6241 ctagtcatat taaaccttat atggtttgta ttactgagaa aaatgagaaa gaggcattag
     6301 attatttaaa tgggttagct ttaacgccga cttatgattg gttgttcgat caaggttata
     6361 ttactttctt ggatgatggt cgtttgattt gcggtactcg actaagccgt tacacatggg
     6421 aaaaacttaa tatcaatcca aatgcaaaaa ataagatgag aatatatccc gaaggaagag
     6481 aaaaatattt ggaatatcat cgcaaatttg tgtttcagga caatatcgat gatttcttgt
     6541 aactaagttt attaatttta ctcaaaacaa aggaaaccga atatgaccat catcgtaaca
     6601 ggcgcggccg gctttatcgg cagcaacatc gtcaaagcac ttaatcaacg cggtattacc
     6661 gacatcgtcg ccgtcgataa tttgagcaaa ggcgaaaaat tcaaaaacct tgccgagtgc
     6721 gaaatcgccc attatctcga caaacacgaa ttcatccgcc aagtgaggga acacatttta
     6781 ccttatcaaa acatcgaagc cgttttccat caaggcgcgt gttccgatac gatgaaccac
     6841 gacggtttgt atatgatgga aaacaactac cagtacacgc tggatttgtt gaactggtgt
     6901 caggacgaac gcattccctt cctttatgcc tccagtgcgg cggtttacgg caaaggcgaa
     6961 atcttccgcg aagagcgcga actcgaaaaa ccgcttaatg tgtacggcta ctccaaattc
     7021 ctgttcgacc aagtattgcg ccgccgcatg aaagaaggtc tcaccgccca agtcgtcggc
     7081 ttccgctact tcaacgttta cggacaacac gaacaacaca aaggccgcat ggcatccgtc
     7141 accttccacc atttccacca ataccgcgaa cacggttacg tcaacctgtt cggcagcaat
     7201 gacggttacg gcaacggcga acaaacccgc gactttgtca gcgtcgaaga cgttgccaaa
     7261 gtcaacctct acttcttcga ccatcccgaa ctttccggca tctacaacct cggtaccggc
     7321 cgcagccaac agtttaacga actcgccgcc gccaccgtca acgcctgccg cgccgccgaa
     7381 ggcaagcctg aaatgagctt gaaagagctg gtggaagaag aacttatccg ctacatcccc
     7441 ttccccgacg cactcaaagg caaataccag agcttcaccc aagccgacat caccaaattg
     7501 cgtgaggcag gttatacgga agaatttttc gatgtcaaat caggcgtcga gcgctacgtc
     7561 aaatggatgc tggaaaattt ggcttaattt gaatgcccgt aaaaaatcgt ctgaaaatat
     7621 caggcgattt tgatttgttt aacttttata tggatttcga tgatgaccga aatgcaacaa
     7681 cgcgcccaac tgcaccgcca aatttggaaa attgccgacg aagtacgcgg cgcggtggat
     7741 ggctgggact ttaaacaata cgttctcggc acacttttct accgctttat cagcgaaaac
     7801 ttcaccgact atatgcaggc aggcgacagc agtattgatt acgccgctat gccggacagc
     7861 atcatcacgc ccgaaatcaa agacgatgcc gtcaaagtta aaggctattt catctacccc
     7921 agccagcttt tttgcaatat tgccgccgaa gcccatcaaa acgaagagct caacaccaag
     7981 ctgaaagaaa tctttaccgc gattgaaagc tccgcctccg gctatccgtc cgaacaagac
     8041 attaaaggcc tgtttgacga cttcgatacc accagcagcc ggctcggcag cacagttgcc
     8101 gacaagaaca aacgccttgc cgccgtcctc aaaggcgtgg cggaacttga tttcggcagt
     8161 tttgaagacc accacatcga ccttttcggc gatgcctacg aatacctgat ttccaactac
     8221 gccgccaacg caggcaaatc cggcggcgaa tttttcaccc cgcaaagcgt atccaagctg
     8281 attgcgcggc tggcggtgca cgggcaggag aaagtaaaca aaatctacga cccagcttgc
     8341 ggctcgggca gcctgctctt gcaggcgaaa aaacagtttg acgagcacat catcgaagaa
     8401 ggcttcttcg ggcaggaaat caaccacacc acctacaacc tcgcccgcat gaatatgttt
     8461 ctgcacaacg tcaattacaa caaattccac atcgaattgg gcgacacact gactaatccc
     8521 aagctcaaag acagcaaacc ctttgatgcc atcgtttcca atccgcctta ttccatcaac
     8581 tggataggca gcgacgaccc caccttaatc aacgacgacc gctttgcccc cgcaggcgta
     8641 ctcgcaccga aatccaaagc cgattttgcc ttcatcctgc acgcactgaa ctacctttcc
     8701 ggcagaggcc gcgccgccat cgtctctttc cccggtattt tctatcgcgg cggcgcagag
     8761 cagaaaatcc gccaatatct ggtggagggc aactacgtgg aaaccgtgat tgcccttgcg
     8821 cccaatctct tttacggcac cggcatcgcc gtcaatatcc tggttttgtc caaacacaaa
     8881 gacaataccg acatccaatt tatcgacgca ggcagcttct ttaaaaaaga aaccaacaac
     8941 aacgtcttaa ccgaagaaca cattgccgac atcgtcaaac tcttcgccga caaagccgat
     9001 gtgccgcata tcgcccaaaa cgctgcccag caaaccgtca aagacaacgg ctacaacctc
     9061 gccgtcagca gctatgttga acccgaagac acacgcgaaa ttatcgacat caaacagctt
     9121 aacgccgaaa tcggcgaaac cgtcgccaaa atcgaacggc tgcggcgcga aattgacgaa
     9181 gtgattgcag agattgaggc ctaggcagtt gttgcatatt ttgcaacaac tgaatgggaa
     9241 agaggccgtc tgaaaatacg ggcggtaacc tgtaagtaat ctttacaagt tcaaatcaag
     9301 aaattttcgg ggaaaaatac atgacaacag aaaacaacgc ttttgaaaac gccaaacaca
     9361 tcgacgaaac aggcaatgaa tattggtcgg tgtgcacctt gcagacaaag tagggtgggc
     9421 ttcagccccc cgaaacaacg gcaaaacagc aggcggattg ttgggctgga gcccacccta
     9481 cggcggataa ccgccccaca tcccgccgca gcgggaaaga aacggaaaac aaccatggat
     9541 atacaaagta aagcgaaaaa attgattgag atgattcaga cggcaccggt ggagtggaag
     9601 ccgttggggg aagtggcaag tataggaaag ggaaagcagc ttaataaatc actacttaga
     9661 gaaaaagata agtatcccgt aatcaatggc ggtattaatc catcaggtta ttggcatgaa
     9721 tacaataacg aagctggcac gattgttatt agccaaggag gagcaagtgc ggggtttgtt
     9781 aattggatgg atatcccttt ttttgcagga gctcacgcat atactattaa agcgaacgaa
     9841 gacaaactat tgcaacggtt tgcatattat ttttaaaaat gaagcaaatt acattcatgc
     9901 aatgtcaata cggagcaggt ataccagcac ttgctaaaag tgaattgcaa aacttactta
     9961 ttcccatccc gcccctagaa acccaacaaa aaattgtaaa aatacttgac aaattcaccg
    10021 agctggaagc ggaattagcc ctgcgcaaac gccaataccg gtattaccgc gactttcttt
    10081 tagattttga caatcaaatc gggggggggg atagctgatg gctatcaatg ccgtctgaaa
    10141 gatgtggttt ggaagacgtt gggggagatt gccgaatatt caaaaaaccg tatttgttcg
    10201 gataaactga acgaacataa ttacgtgggc gtggataatc tcttacaaaa tagagaaggt
    10261 aaaaagttat ccggttatgt tccaagtgag ggaaaaatga cagaatatat tgccaatgac
    10321 atcttgattg gaaatattcg tccgtatttg aaaaaaatct ggcaggcaga ctgcacgggc
    10381 ggaacaaacg gtgatgtttt ggttatccgg gtaacagatg aaaaggttaa tccaaaatat
    10441 ctgtatcagg tattggccga tgataaattt tttgccttta atatgaagca cgccaaaggt
    10501 gcaaaaatgc cgcgtggcag caaagcagcg attatgcaat ataaaatccc catcccacca
    10561 ctccccgaac aggaaaaaat cgtcgccatc ctagacaaat tcgacaccct gacccactcc
    10621 gtcagcgaag gcctgccgta cgaaatcgcc ctgcgccgca aacaatacga atattaccgc
    10681 gaacagctgc ttgcctttcg gtagggtggg cttcagccca ccgaaacagc agcaagctgc
    10741 aacaacagta aaacaacggc aggcggggcg gtgggctgaa gcccacccta cggcgagcgg
    10801 caaccgcccc gcattccgcc acagcgtgtg gaaaccggaa aatatggcgg cggatgctca
    10861 aaacaaagag aaaaaaccga ttgagatgat tcagacggca tcggtggggt agaagtcgca
    10921 gacaaagtag ggtgggcttc agcccaccga aacagcagca agctgcaaca acagtaaaac
    10981 aacggcaggc ggggcggtgg gctgaagccc atcctacgat ggacacaacc gacccgcatt
    11041 ccgccgcagc caaccaaagc agcctgcaca ttttcaggca gcccaatccg aacatcaaac
    11101 cgtttatagt gaattaaatt taaatcagga caaggcggcg agccgcagac agtacaaata
    11161 gtacggcaag gcgagccaac gcagtaccgg tttaaattta attcactata aaacccgaca
    11221 ccgcaaagga cgacccatat gaacctcgaa actaaaccca tcgccgaaac gccgaatttc
    11281 atcgtgctcg accaatatga aaaaatcgaa cagtcgggca gctaccaatc ggaaaaccag
    11341 ttggaagcgg agttaatcgc cgatttgcag aatcagggtt acgaataccg caaggatttg
    11401 aacagccaaa gcaggctgct ggaaaacctg cgcgcgcagt tgcagcggct gaacgatgtg
    11461 gcgttttcag acggcgaatg ggcgcggttt ttgacggaat atctggacag gccgtctgaa
    11521 aacattaccg ataaaacccg aaaaatccac gatgaccata tttacgattt cgcttttgat
    11581 gacggccgtc tgaaaaatat ttatctgctg gacaaaaaaa accttgcccg caaccatgtg
    11641 cagcttatca accagtttga gcagacgggc acgcatgcaa accgctatga cgttaccgtg
    11701 ttggtaaacg gcttgccgct ggtgcagatt gaattgaaaa agcgcggcgt ggcggtgcgc
    11761 gaggcattca atcaggtgca ccgttacagc aaagagagct tcaacagcga aaattcgctg
    11821 ttcaaattcc tgcaaatctt cgtgatttcc aacggcacgg atacgcgcta tttcgccaac
    11881 accaccaagc gcgacaaaaa cagcttcgat ttcacgatga attgggcgcg gtcggacaat
    11941 catccgatta aggatttgaa agactttact gccacgttcc tgcagaaaag cgtattgctg
    12001 ggcgttttgc tgcattacag cgtgttcgat gcgaatgata cgctgctgat tatgcggccg
    12061 taccagattg ccgccgccga acgcattttg tggaaaatca acagttcggc gcaggcgaag
    12121 aattggagcg ggccggaaag cggcggctat gtctggcaca ccacgggcag cggcaaaacg
    12181 ctgaccagct tcaaggcggc gcgtctggcg acggaatcgg cgtttatcga caaggttttc
    12241 ttcgtggtgg acaggaagga tttggactat cagacgatga aggaatacca acgtttttcg
    12301 accgacagcg tgaacggttc ggaaagcacg gcgggcttga aacgcaattt ggaaaaagac
    12361 gacaacaaaa tcatcgttac caccatccag aagctgaaca acctgatgaa gagtgaagat
    12421 aatctgccgg tttatcacca gcaagttgtc tttattttcg acgaatgcca ccgttcgcaa
    12481 ttcggcgaag cgcaaaaaaa cctgaaaaag aaatttaaaa aattctgcca gttcggtttt
    12541 accggtacgc cgatttttca tgaccatatc gttgatggca aactgattat gaaaggcaat
    12601 gcgcttggtt cggagagtac ggaaagcgta tttgggagac agctgcattc ttatgtgatt
    12661 accgatgcca tccgcgatga aaaagtattg aaattcaaag tggattacaa cgatgtgcgc
    12721 ccgcagttca aagccgtgga agcggaacag gacgagaaga aactgagtgc cgccgaaaac
    12781 cgccaagccc tgctgcatcc tgaacgcatc cgcgaaatca cgcaatatat cctgaaccag
    12841 ttcaggcaga aaacacaccg cttgaatgcg ggcggcaaag gctttaacgc catgtttgcc
    12901 gtcagcagcg tggatgcggc gaagtgctat tacgaagcgt tcaaaacaca acaggcaggc
    12961 agcctgcacc cgctgaaagt ggccaccatt ttttcctttg ccgccaacga agagcaaaac
    13021 gccgtcggtg aaattgccga tgaaaccttt gaaccggaag cgatggacag cagcgcaaaa
    13081 gaatttttgc aggctgccat caacgattac aacgcctgtt tcaaaaccaa tttcggcacg
    13141 gacagcaaag cctttcagaa ctactaccgc gatttggcaa aacgggtaaa aaatcaggaa
    13201 gtggatttgc tgattgtggt cggcatgttt ttgacgggtt ttgacgcgcc gacgctgaac
    13261 acgctgtttg tcgataaaaa cctgcgctat cacggcctga tgcaggcgtt ttcgcgcacc
    13321 aaccgcattt acgatgccac caaaaccttc ggcaacattg tctgcttccg cgatttggag
    13381 caggcaacca ttgatgcgat taccttgttt ggcgacaaaa acaccaaaaa tgtagtgctg
    13441 gaaaaaagtt acgaagaata catgaacggc tatatcgaca gccagaccgg cgaagcgcgg
    13501 cgcggctatt tggatgtggc aaaagaattg cgcgaacgtt tccccgatcc cgacgaaatc
    13561 gaaacggaaa aagacaaaaa agattttgcc aaactcttcg gcgaatacct gcgggcggaa
    13621 aacgtattgc agaactacga tgaatttgcc gcgctgcgcg agttgcagaa tgtggatgcg
    13681 gcggacgaag atgcgatgaa ggcgtttcag gaaaaatact acctgagcga tgaagacgtg
    13741 caggaaatgc ggcaagtgcc gatgccgtct gaaagggcgg tgcaggacta ccgttccgcc
    13801 tacaacgaca tccgagactg gctgagccgc caaaaagcag gcgaacagaa agaacagtca
    13861 aaaatcgatt gggacgatgt ggtttttgag gtggatttgc tcaaatcgca ggaaatcaat
    13921 ttggattaca tcttgcaact ggttttcgag catcacaaaa aaatcaaagg aaaagcggag
    13981 ctggtggaag aaatccgccg catcatccgc gccagcatcg gccaccgcgc caaagagggt
    14041 ctgattgtgg attttatcaa cgatacggat ttggataaag tccccgacgt tcccgccata
    14101 ctggaaacct tttacaccta cgcgcaagag gtaatgcggc acgaagcggc aggattgatt
    14161 gccgccgaag acctgaacga aaccgccgcc aaacgctatt tgaccggctc gctcaaacgc
    14221 ggctatgcca gcgaaaacgg tacggaactg accgaaaccc tgccgaaaat gagtccgctc
    14281 aacccgcaat atctgacgaa gaaacaaagt gtttttcaaa agattgcggc gtttgtggag
    14341 aagtttgccg gaatagggac cgatatttga caaaatgccg tctgaacctt tcagacggca
    14401 tttttgattt tatgcggagg cggtttttat tttgaccttg cttttcttaa acttcaacac
    14461 ggcttcttct tttgccgcat cccagtctat ccgcacgaag ccgccgtcgg atagtttgcc
    14521 gaacaggagt tcgtcggcga gcggtttgcg gattttttcc tgaatcaggc ggtgcatcgg
    14581 gcgcgcgccc atttgcgggt cgaaaccttt ttccgccaga tatttgtgca atgccgacgt
    14641 gaattcggct tcgacttttt tgtcgaggag ctggtgttcg agctggagca ggaatttgtc
    14701 cacgactttg acgatgatgg gttcggacaa gggtgcaaac gggataatcg cgtccaagcg
    14761 gttgcggaac tcgggcgtga agagcttgtt gattgcctgc atttcgtcgc cgcgttcgcg
    14821 cttggcggta aagccgaggc tggggcggct gaggctttcc gcgcctgcgt tggtggtcat
    14881 aatcaggatg acgttgcgga aatcggcact cttgccgttg ttgtcggtca gtttgcctgc
    14941 gtccatgact tgcaggagga cgttgaaaat gtcggggtgg gctttttcga tttcgtccaa
    15001 gagcaatacg caatgcggct gtttgttgac ggcttcggtc aaaaggccgc cttgttcaaa
    15061 gccgacgtag cccggtggcg cgccgatgag gcgcgatacg gcatggcgtt ccatgtattc
    15121 ggacatatca aagcgttgca gcggtacgcc cagcgagtag gcaagctgtt tggcggcttc
    15181 ggttttgccc acgccggtcg ggccggagaa gaggaagctg cctatcggtt tgtcgggcag
    15241 ggcaaggccg gaacgcgaca ttttgacggc ggcaaccagc gcgtcgatgg cgttttcttg
    15301 accgtaaacc atgtttttca aatcgcgacc gaggaattgc agcacttgtt tgtcgtcgtg
    15361 cgacacggtt ttttcgggaa tccgcgcaac tttggcgatg acggtttcaa tttgtgcttt
    15421 gccgatgact tttttctgtt tggatttggg cagaatccgt tgcgccgcgc ctgcttcgtc
    15481 catcacgtcg atggctttgt cgggcaggaa acgctcgttg atgtagcgtg cggagagttc
    15541 ggcggcggct tcgagtgcgc cttgcgtata gcggacttgg tggaaggctt caaacatcgg
    15601 cttcaagccg cgcaggattt gtacggtttc ggaaacggtg ggttcgacca cgtcgatttt
    15661 ttggaagcgg cggcttaagg cgtggtcttt gtcgaaaatg gtgcggtatt cgtcgtaagt
    15721 ggtcgcgccg atgcagcgca gcgaaccttt tgccagcgcg ggtttgagca ggttggacgc
    15781 gtccatggtg ccgccgctgg tgctgcctgc gccgatgatg gtgtggattt cgtcgataaa
    15841 caaaatggcg tgcgggattt tttcgagctg tttcaagacg gatttgaccc gcgcttcaaa
    15901 gtcgccgcgg tatttcgtgc ccgccaacag cgagcccata tccagcgcgt acacttcggc
    15961 atctttgagc gcgtctggaa tgccgccgtt gacgatttga tgtgccaaac cttccgccag
    16021 cgcggttttg cccacgcccg cttcgccgac caaaagcgga ttgtttttgc ggcggcggca
    16081 taggatttgc accagccgtt ccatttcgtg tttgcgacca atcaaagggt cgatacggcc
    16141 ggctttgact tcggcgttga ggttgacggt gtacgccgat aaagggtttt tgcccggttt
    16201 ggtgcggttt tcattatcgt cgtccatgcc gtctgaagaa tagttgccat cgtcttcatc
    16261 ttcatcggga gagccgtggg caatacagcg caaaacttca aaacgcgtaa ccgattgcag
    16321 cttgaggaaa tagacggtgt ggctgtcggt ttcgctcatc agcgcgacca aaacgtccaa
    16381 cggttcgact gcggcttttc cggcagactg ggtatgcacc atcgcccgtt gcatcacgcg
    16441 ttggaagccg agcgtgggcc gtgtttcgac cgtgtctaaa aggtgttcgg gaatcagggg
    16501 ggtgttttcg gcaacgctgg cggcgagctg ttcggacacc actttcaaat ccgcgccgca
    16561 gagttttaag acgttcggca cggaggcatc ttcttcgatg agtaccaaaa gcagatgctc
    16621 gaggctgata aattcataat gagccttacg cgcctcgcgg taaagctgct gcaaaatctg
    16681 ttccaattcg ggtgcaagca tattaaatct cctcgacaat acattgcagc ggatgccctt
    16741 cggcttttgc ccgctgcatg acttgttgct gtttggtttg ggcaatatcg cgcgtgtaag
    16801 tgccgcacag gcctttgcct tcgtgatgaa ccaagagcat taccgctacc gcctgttctt
    16861 gtccgagcat gaagatttcg gtcaggattt caacgacaaa ttccatcgtg gtgtaatcgt
    16921 cgttcaatag gaaaacgccg taacgtttcg gcggcagggt gttcagacgg tgcaagagcg
    16981 tgtcggattg gtgttgcgcg gtcatagtgt gtcccatttg aaagccgcgt tcagacggca
    17041 tttttgttgt attttcggta cttttgccta ttttcccact tttttgaaaa catagcttga
    17101 cgttttgtct taacaaatgt aaaaagacgg ttaagcagga ttgggcatcc gccgagtata
    17161 tcaagctgga aggaacgcca gacggctcgg tttgccgtat gccttgtttt gctgattttg
    17221 ttaatttttg agtataggaa gtttctaatg gcaaccggta tcgtaaaatg gtttaacgac
    17281 gctaaaggtt ttggtttcat cacgcctgat gaaggcggcg aagatttgtt cgctcacttc
    17341 tcagcaatca atatggaagg tttcaaaacc ctgaaagaag gccaacgcgt ctctttcgac
    17401 gtaaccaccg gccctaaagg caaacaggcc gccaacattc aggctgctta attcctgatg
    17461 tacggtcaaa tgtatatttg aaaacggcgg gacaggcaat gtcccgccgt ttttgtctgc
    17521 cgttttgccg gcggcggaaa aaccccaatc cccgcacgcc ttatcctgaa cttgtgtgta
    17581 ccctgttgtg gacaagtggc ttagtatttt gacggataag ggaaaatcag tgctgatgaa
    17641 aaaatgtgca atgttgtcgg caaaaggcgg tgcggcataa aacggcaaac gggcaggcac
    17701 ggggcaaaac gtgctgcctt cgtcttcaga cggcatcggc agggcgttca gcttccggca
    17761 accgtcatcc ccgcaatcag aatcgagccg attttgttgg acgaacgccg caaagcgtca
    17821 tccgccacgc cgacaatgtc gcggtacata tcctgcaagc gtccggctac ggtaatctcg
    17881 tggacggggt aggcaatcac gccgttttcc acccaaaaac ccgccgcgcc gcgcgagtag
    17941 tcaccggtaa tggtgttcgc gccctgtccc atcagttcgg taaccaataa tcccgtcccc
    18001 atttctttca gcaggtcgga ttgcgtttcg tgcgtatggt tcaaatacag gttgtgcgcg
    18061 ccgccggcgt tgcccgtggt ctgcataccg agtttgcgcg cgctgtaact gctgaggaaa
    18121 tagccttcga cgatgccgtc gcggataacg aaacgcggcc gcgtggcgac gccttcggaa
    18181 tcgaaatagg tgctgccgaa ggcgcgggga atatgcggct cttcgcgcag gctgaggaaa
    18241 tcgggcagaa cctgtttgcc tatgctgtcg atcaggaagc tgctttggcg gtagagcgag
    18301 ccgccggaca gtgcgccgac gaggtgtccg atcagacctc ccgaaacggt cgcatcgaat
    18361 aggacggggt agcttccggt cggaatgctg cggctgttca gacgacgtaa ggtacgttcg
    18421 gcggcggttt gaccgatttg cgtcgggctg tccatgtctt gatggcggca ggaggaatcg
    18481 taccagtagt cgcgctgcat gccgttttcg tcggcggcaa cgacgctgca tgaaatgctg
    18541 tggcgtgtgc cttgctggtg tgcggcaaaa ccgtgggtgt tgccgtaaac gtattggtaa
    18601 tgtccggttt gcaccgccgc cccttcggag ttttcgatgc gctcatcctc gttcagggcg
    18661 gcttgttcgc attgttttgc caactcgacg gcggcttccg tatccaaatc ccattcgtgg
    18721 taaaggtcgg ggtcgccgat gtgttgcgcc ataagcgaag cgtctgccaa acctgcgcaa
    18781 tcgtcttcgg cggtgtagcg ggcgatgtcg atggcggctt tgacggtgtc ttgcagggct
    18841 ttttcggaga agtcggcagt gctggcgcgg cctttgcgtt tgccgacgta aacggtaatg
    18901 tccagcgact tgtcctgctg gaactcgatt tgttcgattt cgcccagccg cacgctgacg
    18961 ctttgtccca atgattcgct gaaatcggct tcggcggcgg tcgcgcccgc tgcttttgcc
    19021 aagtcgagcg tgcggcggca gaggtcgaga agttcggaag cggtgtggtt gaacagcata
    19081 gaaatctttc ttggtggagc gttgcggcat tttaaccgtt tcgggcggca ggggcaaaag
    19141 cgtgccgttt gcagggcgga cggtgcaaaa tgccgtctga acgcggaatc attctgttaa
    19201 aatgcgcccg tcggataaaa tttgagaatg aagatgtttg aacaagaaga cgaatgggtc
    19261 agcaaaaccc aaatgaaaaa gcagatgaac ggtttgcagg atttgggtat ggagctgacc
    19321 aagctctcaa gcgacacgtt gaaaaaaatc ggcttggacg aagatttgta cgaagccgtc
    19381 gttacctata aaaaaatcac atccaacggc gcgctcaagc ggcaggcgca atttatcggc
    19441 agactgatgc gcgataccga tcccgcgccc atcgaggcat ttcttgccaa gctgcgcggc
    19501 gacgatgcgg tgcacaatgc ctttttgcaa cgcgtggaac aggcgcgcgt acggctgttg
    19561 gcagacgacg gcgcgttgac gcagtttatg tcggattttc cgcatgcgga cgcgggcaag
    19621 ctgaggacgc tcgtccgcaa caccaaaaaa gagcaggagc aaaacaaacc gccgaaaaac
    19681 ttccgcgcgc tgtttcaaga attgaaaacc gtgatggaaa acggggacgc ggaaatttag
    19741 gcatattttc agacgacatc cgccgttatt tagattggag gataaaatgt tgttccgtaa
    19801 aacgaccgcc gccgttttgg cggcagcctt gatgttgaat ggctgtacgg caatgatgtg
    19861 gggcatgaac gacccgttca gccaaacgac cgcctataaa cacgttgaca aggatcaaat
    19921 ccgcgccttc ggtgtggttg ccaaagacaa tgcccaattg gaaaagggca gtctggtgat
    19981 gatgggcggg aaatactggt tcgtcgtcaa tcccgaagat tcggcgaagc tgacgggcat
    20041 tttgaaggca gggctggaca aacccttcca aatagttgag gataccccga gctatgctcg
    20101 ccaccaagcc ctgccggtca aactcgaatc gcctggcagc cagaatttca gtaccgaagg
    20161 cctttgcctg cgctacgata ccgacaagcc tgccgacatc gccaagctga aacagctcgg
    20221 gtttgaagcg gtcaaactcg acaatcgaac catttacacg cgctgcgtat ccgccaaagg
    20281 caaatactac gccacaccgc aaaaactgaa cgccgattac cattttgaac agagtgtgcc
    20341 tgccgatatt tattacacgg ttactgaaaa acataccgac aaatccaagc tgtttgcaaa
    20401 tatcttatat acgccccctc cctttttgat actggatgcg gcgggcgcgg tactggcctt
    20461 gcctgtcgcg gcgttgattg cagccacgaa ttcctcagac aaatgaacgg caatgccgtc
    20521 tgaaaagcct tcagacggca tttcaaacac atccgcactg taaagttcca cgctatgtcc
    20581 gtcaaaatcc aaacccgatc cgtcaatacc gacgttttca atcatttgct caccgccggc
    20641 gccgatcctt taatcgcccg gctttgtgcc tcgcgcggtg tgcaaagtcc tgccgaattg
    20701 gacgacaaac tcgcttccct cctgccttat caaacgctga cgaattgcga agccgccgcc
    20761 cgccgtttgg cggatgcggt tgagcgtcag gaaaaaatct tgattgtcgc cgactacgat
    20821 gccgacggcg cgaccgcgtg tgccgtcggt ttggacggtt tggcggcgat gggggcaaaa
    20881 gtagatttcc tcgtgcccaa ccgctttgaa cacggctacg gtttaacgcc cgagctggcc
    20941 gaaatcgccg ccgcgcaagg cgtggatttg ctgattacgg tcgataacgg cattgccagc
    21001 atcgcaggcg tggcgcgtgc gcaggaattg ggtttggatg tgattgtaac cgaccaccat
    21061 ctgcctgccg agaccgtgcc agactgcatc atcgtcaatc cgaaccaaaa aggctgcggt
    21121 ttccaaagca aaagcttggc gggcgtgggc gtgatttttt atgtactgat ggctttgcgt
    21181 gccgaactgc gccgccgcaa ttatttttca gacggcctaa aagagccgaa tctgggcgac
    21241 cttttggatt tggtcgcact cggtaccgtt gccgacgtcg tctccctcga tcacaacaac
    21301 cgtatcctcg tgtcgcaagg tttgaaacgg atgcgttcgg gcaaaatgcg ccccggtatc
    21361 cgcgccttgt ttgaagtggc gcggcgcgac tggcgcaaag cgcagccgtt tgatatgggc
    21421 tttgcgttgg ggccgcgcat caatgccgcc ggacggttgg acgatatgtc ggtcggcatt
    21481 gcctgtctgt tggcgcgaga tgatgtggaa gcgcaggaac tggcggctca gttaaacaac
    21541 ctcaatatcg agcgtcgcga aatcgagcag tctatgctgc aagacgcgct gaatgctttt
    21601 cctgaaaccc tgccttcagg tcagacgact ttggtggcgt atcgcgacga cttccatcaa
    21661 ggtgtggtcg gcattgtcgc cagccgcctc aaagaccgtt tttatcgtcc gaccatcgtg
    21721 tttgcgcctg ccgacaacgg cgaagtacgc ggttcgggac gttccattcc caatttgcac
    21781 ctgcgcgatg ctttggactt ggtgtccaaa cgccatcccg atttgatttt gaaattcggc
    21841 ggacacgcga tggcggcggg tttgagcata cttgaacaca acattcccgc gtttcagacg
    21901 tcctttgaag aagccgtgcg cgaaatggtg tgcgaagacg atttgtcgca aaccttcatc
    21961 accgacggca gcctgcccgc ctgcgacatc acgttggaac aggcgcaaaa ccttgcccgt
    22021 cacgtttggg ggcagggctt cgcgccgccg agctttaccg acgagttcca cgtcgtccgc
    22081 cagcaacctt tgggcgcgga gggcaaacac aaaaaagtct ggctgcaaaa agacggctgc
    22141 gaatttgaag ccatgttttg gcgttgcagc gaagacatcc ccgaatacat ccgcacggtt
    22201 taccgccccg ttgccaacga atggcgcaac aatctcgaat tgcagctgta tatcgattac
    22261 tgggaagcgg tgtagaggcg gcggaacgcc gtttgaatgt gatttctgtt ccttcatttg
    22321 cctgtttgta cgacgggaat gttcccaatc ggaaaagaaa gtaaagggcg ttaatttttg
    22381 ttaatcatcc cttcttaggg acgcaatata taaggtgcat caaatttcag actccggcgg
    22441 atttgcattt gaagtgcaac tttccctaac agaaaaaggc cagtatgcgg tagcatacg
//
LOCUS       DAXHMX010000014        21828 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHMX010000014 DAXHMX010000000
VERSION     DAXHMX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.014"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(108..1031)
                     /gene="era"
                     /locus_tag="ACFM4S_000411"
     CDS             complement(108..1031)
                     /gene="era"
                     /locus_tag="ACFM4S_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6050158.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1078..1797)
                     /gene="rnc"
                     /locus_tag="ACFM4S_000412"
     CDS             complement(1078..1797)
                     /gene="rnc"
                     /locus_tag="ACFM4S_000412"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6050159.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1964..2287
                     /locus_tag="ACFM4S_000413"
     CDS             1964..2287
                     /locus_tag="ACFM4S_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6050160.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2335..2811
                     /gene="ribH"
                     /locus_tag="ACFM4S_000414"
     CDS             2335..2811
                     /gene="ribH"
                     /locus_tag="ACFM4S_000414"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6050161.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2889..3314
                     /gene="nusB"
                     /locus_tag="ACFM4S_000415"
     CDS             2889..3314
                     /gene="nusB"
                     /locus_tag="ACFM4S_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6050162.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3681..4715)
                     /gene="pyrC"
                     /locus_tag="ACFM4S_000416"
     CDS             complement(3681..4715)
                     /gene="pyrC"
                     /locus_tag="ACFM4S_000416"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6050163.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVVPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATSELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFTSKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4775..5380)
                     /locus_tag="ACFM4S_000417"
     CDS             complement(4775..5380)
                     /locus_tag="ACFM4S_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050164.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSCVRHQDFARVAVDLF
                     RSACVLTQGSFEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYL
                     SLPRDCCSVKAERADSAVLLEHFDGLVRQPPKNTNIELRAEQSVQALFETRQISYAWR
                     ESGSPEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            complement(5381..5605)
                     /locus_tag="ACFM4S_000418"
     CDS             complement(5381..5605)
                     /locus_tag="ACFM4S_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6050165.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            5714..6325
                     /locus_tag="ACFM4S_000419"
     CDS             5714..6325
                     /locus_tag="ACFM4S_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6050166.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHDK"
     gene            complement(6385..7257)
                     /gene="accD"
                     /locus_tag="ACFM4S_000420"
     CDS             complement(6385..7257)
                     /gene="accD"
                     /locus_tag="ACFM4S_000420"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6050167.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPESFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(7294..8079)
                     /gene="trpA"
                     /locus_tag="ACFM4S_000421"
     CDS             complement(7294..8079)
                     /gene="trpA"
                     /locus_tag="ACFM4S_000421"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6050168.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            complement(8506..8598)
                     /locus_tag="ACFM4S_000422"
     tRNA            complement(8506..8598)
                     /locus_tag="ACFM4S_000422"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8562..8564),aa:Ser,seq:gct)
     gene            complement(8755..8955)
                     /locus_tag="ACFM4S_000423"
                     /pseudo
     CDS             complement(8755..8955)
                     /locus_tag="ACFM4S_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(9057..9374)
                     /locus_tag="ACFM4S_000424"
                     /pseudo
     CDS             complement(9057..9374)
                     /locus_tag="ACFM4S_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(9397..10158)
                     /gene="kdsB"
                     /locus_tag="ACFM4S_000425"
     CDS             complement(9397..10158)
                     /gene="kdsB"
                     /locus_tag="ACFM4S_000425"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6050169.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMRVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            complement(10155..10337)
                     /locus_tag="ACFM4S_000426"
     CDS             complement(10155..10337)
                     /locus_tag="ACFM4S_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6050170.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            complement(10406..10987)
                     /locus_tag="ACFM4S_000427"
     CDS             complement(10406..10987)
                     /locus_tag="ACFM4S_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6050171.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            complement(11193..12227)
                     /gene="lpxK"
                     /locus_tag="ACFM4S_000428"
     CDS             complement(11193..12227)
                     /gene="lpxK"
                     /locus_tag="ACFM4S_000428"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6050172.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLDSVDTVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            complement(12358..12537)
                     /locus_tag="ACFM4S_000429"
     CDS             complement(12358..12537)
                     /locus_tag="ACFM4S_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050173.1"
                     /translation="MKEMETVRYHIGDMPETSKQTAPVMTTGQWVLTMIVFMIPLVNF
                     CLGVRQRQPEPRQFL"
     gene            complement(12620..13900)
                     /locus_tag="ACFM4S_000430"
     CDS             complement(12620..13900)
                     /locus_tag="ACFM4S_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6050174.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATSKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLVDAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRSCVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            complement(14122..14742)
                     /gene="tmk"
                     /locus_tag="ACFM4S_000431"
     CDS             complement(14122..14742)
                     /gene="tmk"
                     /locus_tag="ACFM4S_000431"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6050175.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            complement(14802..15797)
                     /gene="mltG"
                     /locus_tag="ACFM4S_000432"
     CDS             complement(14802..15797)
                     /gene="mltG"
                     /locus_tag="ACFM4S_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6050176.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLYTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            15881..16453
                     /gene="ampD"
                     /locus_tag="ACFM4S_000433"
     CDS             15881..16453
                     /gene="ampD"
                     /locus_tag="ACFM4S_000433"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6050177.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            16647..17933
                     /locus_tag="ACFM4S_000434"
     CDS             16647..17933
                     /locus_tag="ACFM4S_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6050178.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVCDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            18081..20606
                     /gene="ligA"
                     /locus_tag="ACFM4S_000435"
     CDS             18081..20606
                     /gene="ligA"
                     /locus_tag="ACFM4S_000435"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6050179.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETTDKTVAESDQTPSEG
                     SSVGASGKHKKQPVKWAENILGGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            20663..>21828
                     /gene="hemW"
                     /locus_tag="ACFM4S_000436"
     CDS             20663..>21828
                     /gene="hemW"
                     /locus_tag="ACFM4S_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6050180.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         5216 a         6445 c         5340 g         4827 t
ORIGIN      
        1 atgcacagat aatatcaacc cgtttttaac aaagatattc ccgattgaaa caaaatgccg
       61 tctgaaacct gttcggacgg catttgcgta aagttcagca agaaaaacta caaacccagc
      121 tcgcgcagga agcggatgtc gtccgcccaa ccggatttga ctttgaccca gaccttcaaa
      181 aatactttgg tatcaaacag tttttccata tccaaccgcg cttcggtgga aattttcttc
      241 aaacgctccc cgcctttgcc gattaaaatc gccttttggc tttccttatc gaccaaaacg
      301 gcgatataga tgcggttcaa accgtcttcc tcttcaaact gctccacttc gacgttcatc
      361 gcataaggca attcctcgcc caaatagcgg aacaattttt cacgcacgat ttccatcgct
      421 aaaaaacgcg ccgatttgtc cgtaaccata tcttcgggat acatcggcac gctttcgggc
      481 agatacggct tgagcagctc caacaggttg gcaatccgca atccgtgttt cgcactgacc
      541 gcctccgccg ccgcaaattc aaattcggcg cgcacttggg caacaaacgc ctccagcgcg
      601 taacggtctt tcgccttgtc cttgtcgatt ttgttgacca ctaaaatgac cggcgtgtgc
      661 ttgggcagtt gtttcaacac gacacggtcg gcatcggtaa agcgcatcgc ttccacgacg
      721 aaaaccacca catccacgcc gccaagcgcc tcggtaacgt tttgattcag cctgtcgttg
      781 agcgcgttgc ggtggtcggt ttgaaagccg ggcgtatcga caaacacgaa ctgcgcggta
      841 tcgtcggtat aaatccccgt tacgcggttg cgcgtcgtct gcgccttttt gctggtgatg
      901 ctgattttct gaccgatgag atggttcatc agcgttgatt tgcccacgtt cggacgaccg
      961 acaatcgcca cgaagccgca acggtatccg ccggcggcgc gttcccctgc aaggaaggtt
     1021 tcaatatcca tattcgcttt catatccaca tttcagacgg catattcacg cgccgcctca
     1081 tttctttttc ttcttcagcg gcagcttctc ttccagccat ttcaaagcct ctttcgccgc
     1141 ctcctgttcc gccgccttgc ggctcgtccc tttggcacgg cacacgaaac ccagttcgcc
     1201 caaatcgcag gaaaccacaa acatactgtc gttggcatgg ccgatttgct cttcgatacg
     1261 gtatttcggc aaagcaaagc ggcgtgcctg caacgcctcc tgcaaagcag ttttgccgtc
     1321 ttttgcctga ttttggaaat cggcacaccg gacgcggtcg gcaaacaaat ggcgcaccac
     1381 cttttccgcc gtgttgaaat cggcatcgaa gctgaccgcc gcaaacatcg cctccatcgc
     1441 gtccgccagt atcgaaggcc gtctgaagcc gccgctcttc aactcgcccg cccccaagta
     1501 cagaccgtcg ccgacattca tttccgccgc catttccgcc agcacgccct cattgaccag
     1561 acttgcccgc aaccgcgaca actcgccctc ggtcaacttc ggaaacgtgt caaacagcat
     1621 ccgcgccacc gtataattca aaatcgaatc accgacaaac tcgaaccgct cgttgtgctt
     1681 cgcatgatgg ctcctgtgcg tcaaagcctg ccgcaaaagc gaaatatcgc ggaacgcgta
     1741 gcccagtctc ttctgtatcg ccgcgtgtgc ctgctgtttc aaaacatcgt ctttcattac
     1801 cgtcttccgc aaaaaacagc ccgaatgcgt ttccatactc caaaaataca gaaacacctt
     1861 aaggctgcaa acggcgcaca ttgtaccacc aaaccgccgc cgtctgccga caacgcacga
     1921 atcgtggtaa aatccgtaag ttttccccca acgtattccc gtaatgaacg catttgcaca
     1981 agcactttcc tcggcactcg accgctgtat cgccaccaac accgtcgcca aacaaaaccg
     2041 gcccgtcggc ttcctctacc gcgaagcccc cgtttttgaa aacgacagcg gctggcgttt
     2101 cttcagcggc gacgagaccg acgaatacac cgacgatccc gacaacttca gcatcgtcag
     2161 cctcgccgac atcgccaaaa ccaaccctga aaccgccgca ctgctttccc aacccgaagg
     2221 cagcgcatgg gagcttgccg aagacggcac attccaaacc gtcgccgact ggcagccgca
     2281 agactgaaac cttcagacgg cattccgaaa cctccgaata ccaaaggaca cccaatgaac
     2341 accatcgccc ccaacctcga cggcaaacac ctccgcatcg gcatcgtaca ggcacgtttc
     2401 accaacgaaa tcggcagcga aatgctcaaa gtctgctgcc gcaccctcca agaattgggc
     2461 gtggcagacg aaaacattac cgtcgccacc gtacccggcg cacttgaaat ccccatcgca
     2521 ctgatgaacc ttgcctcttc cgaaaagttt gacgcactga ttgccatcgg tgtcgtcatc
     2581 cgtggcgaaa cctaccattt cgagctggtt tccaacgaat ccggagcagg ggttagccgc
     2641 gtcgcactcg actacaacat cccgattgcc aatgccgtcc taaccaccga aaacgacgcg
     2701 caggcaatcg aacggattga agaaaaagcc tcggatgccg ccaaagtcgc cgtcgaatgc
     2761 gccaacctcg tcaacctgct gctcgaagaa cagtttgaag acgaagaata acaaacaggg
     2821 cgtacaccaa tacgcccttt ccgtttcaga cggcataccg tgccgcccaa ccgtttcaag
     2881 gaaaaatcat gaaaacagcc cgccgccgtt cccgcgagct tgccgtacaa gccgtttacc
     2941 aatcccttat caaccgcacc gccgcgcccg aaattgctaa aaacatccgc gaaatgcccg
     3001 actttgccaa agcggacgaa gaattgttca acaaactttt cttcggtacg caaaccaacg
     3061 cagcggacta catccaaaaa atccgccccc tgctcgaccg cgacgaaaaa gacctcaatc
     3121 ccatcgaacg cgccgtcctg ctgaccgcct gccacgagct gtccgccatg cccgaaacac
     3181 cctaccccgt catcatcaac gaagccatcg aagttaccaa aaccttcggc ggcacggacg
     3241 ggcacaaatt cgtcaacggc atcctcgaca aactcgccgc ccaaatccgc cccgacgagc
     3301 ccaaacgccg ttgatgcatc acaaatttaa agaaagttct atgtccttat ttctgctttc
     3361 tttgtttcgt cattcccacg caggtgggaa tttggttttt tgaattttgg gtatttcaaa
     3421 taaattctga aacttcaatc tcgtcattcc cgcgcaggcg ggaatccggt tttttgagtt
     3481 gctgccgttt ttgataaact ttggtcgcat cgaatttctg gattcctgcc ttcgtgggaa
     3541 tggcgaaatt tgaaaattgt gggaatttat cggaaataac cgaaacgtaa caaactcaaa
     3601 cgccgttgac gtatcaatca gtcagcaaca caaatgccgt ctgaaaaacc tttcagacgg
     3661 cattttgctt gaccgttcaa tcaatactgt accgtccagc cgatttcgcc gcccgcgcgc
     3721 atcgggacaa gttcgccgtc gccgtaggga acacttgcgg ggacggtttg gctttgtttg
     3781 acgagggtga tcgtgccggc gttttccgga atgccgtaaa accttgcgcc gtttttcgag
     3841 gtaaaggctt cgagtttgtc caacgcgcct gctttttcaa acacttcggc gtaaagctcg
     3901 atggcggtca tcgcgctgaa cataccggcg caaccgcagg cgttttcttt agcggatttg
     3961 gcgtgcggcg cggaatctgt gccgaggaag aatttgtgcg ccttctcgcc ggtaacggcg
     4021 gcaaccaatg cctgacggtg ggtttcgcgt ttgagtacgg gcaggcagaa atgatggggg
     4081 cgcacgccgc cgaccaagag gtcgttgcgg ttgagcaaga ggtgttgcgg ggtaacagtg
     4141 gcggcaacgt tgtcgcctgc ttccaaaacg aggcgggcgg cttcggcggt ggtgatgtgt
     4201 tcgaacacga ctttaagatt tggtacttgc gccaaaacgg gtttcatcac gcgcccgata
     4261 aaggcggctt cacggtcgaa gatgtcgatt tcggggtcag ttacttcgcc gtgaacaagg
     4321 aacaggatgc cctgtttcgc catttcttcc aaaacgggga taagtttgaa caggtcggtt
     4381 acgccggaat cggaattggt ggtcgcgccg gcggggtaga gtttgaaggc aacgatgccg
     4441 gcagctttgg cttcgcgtac aagttcggac gtggcgttat cagtcaaata aagcgtcatc
     4501 aacggctcaa acgcgctgcc ttcgggcaac gccgccataa tgcgcgcttt gtaggcaagc
     4561 gcgtcggcaa cgctgacgac aggcggtttg agattgggca taatcacggc gcgccccatc
     4621 tgtctggcgg tataaggcac aacggctttg agcgcgtcgc cgtcgcgcag gtgcaggtgc
     4681 atatcgtcgg ggcggataat ggtcagggtt tgcatggttt tcctttgttt tatttgaatg
     4741 ttcagacggc atgccgactt tatgccgtct gaagttaggg gagaattagg ggtttcccgt
     4801 tgtgggaacg gtgcgtcagg ttcaaaactg cctgtacggg caaatccggg gaatcggcgg
     4861 tttgtgcggt caatacggtt tcttcggggc tgcccgattc ccgccaggca tagctgattt
     4921 gcctggtttc aaacagtgcc tgcaccgact gttcagccct caattctata ttggtatttt
     4981 tcggcggctg tcggacaagt ccgtcaaaat gttccaaaag cacggcactg tctgcccgct
     5041 cggctttcac actgcaacag tcgcgcggca ggctcaaata ataaatgccg ccatcgcgtt
     5101 caagcgtcca caacgcctgt acatcgggtt ctgcaacagc gggcggcaga cgggacaggt
     5161 cttcctttcc gacagggaca aaatgtccga cagcggcaaa cttggaaact ggttcgaaac
     5221 tgccttgagt caggacgcag gcactccgaa acaaatcgac ggctacacgt gcgaaatcct
     5281 gatgccgtac acaggattgc cggttttggg cattacacgc agcaagcaca aatgccgaca
     5341 gcaataacat caatcgggac atccgcattt cagacggcat ttatttgtgc ttgacgacca
     5401 gcgtgaacac gccgtcctgt tcggaagcat ccaacagcac atgaccggtt tggcggcaaa
     5461 aagcctcaaa atcccccggt gcgccgccgt cggttgccag aacggtcaac acgtcgccct
     5521 gctgcatttg cgccaaagcc tttttcgccc gcaaaatcgg caggggacat ttcaatccgg
     5581 ttacgtctaa agtttcgctg ttcatcgttt gtaccgttta aaaaatggga atcgggggga
     5641 gattataccc cagttgcttg cagccccctt tgccaaccca taaaataacg ccgttttcaa
     5701 tttggagaaa cttatgcgcc gcaccgccct cctgctgctc gcactcaccg ccggcacgtc
     5761 ccttgctgcc ggcttcaacc aaaaagacac gccgatcaac acccgctacc gcgaaacgcc
     5821 cgaagaagcc gccgcccgcg aatttaaaga acataccgcc aaactgccgc cgctgcccga
     5881 tacgcactcg gacggctggt ttgacattta tgtggatgaa aattacggca aacagccgaa
     5941 aatcctgctc gacagcctgc aaattatgcc cgcgcccgac ggcagcatcc gctacattct
     6001 caatatccgc tccgacaaag gctacgacaa cctgactgcc gaaggcatat tctgcgcccg
     6061 cttctccatc aggttcggca acggcaaacc ttcctcctat aaagtgttcg gctacggcga
     6121 cacggtcaac cgccgctgga tacagccccg aaacgccgaa tggaaaccga taggcggtac
     6181 attaagccgc aacgacgcac tgcgcaccgt cctgtatcag gcattctgcg agggcggtgt
     6241 acccgccgac acgaaaggtt tggtacaacg cctgaaagaa cgtgccggac gctatgcccc
     6301 gtcgatgaag ccgcacgaca aataaccaga cacaaaaata ccgtctgaaa catcggtttc
     6361 agacggtatt cgattcatca gccatcaagc ggcggaaact ttgtcctgac ggcacaacag
     6421 cgtaatcaaa tcactgatgc gccgcttcat atcgcggcgg tcgacaatct ggtcgattgc
     6481 gcctttttcc agcaggaact cggcgcgttg gaagctttcc ggcagcgttt cgcgcaccgt
     6541 ctgctcaatc acgcgcggac cggcaaaacc gatcagcgcg ttcggttcgg caagcacgac
     6601 atcgcccaaa aatgcgaagc tggcggatac gccgcccata gtcggatcgg tcaacaccga
     6661 tataaatggc aggcgttttt ccgtcagcaa atgcagcgcg gcactggttt tcgtcatctg
     6721 catcaatgag ttcacaccct cctgcatacg cgcaccgccg gaagccgcca cacagacgaa
     6781 cggacaattg tcggcaaccg cccgacggat accttgtacg aatcgttcgc ccacaaccga
     6841 acccatcgaa ccgccgataa agcggaattc aaacgcggca acgacgacgg gcagaccgtt
     6901 catcttgcct ttcattacca ccaaagcatc atcttccccg gtcagcttgc gtgccgcact
     6961 caaacgatcc ggatattttt tgccgtcttt aaacttcaaa ggatcggtcg gtttgacatt
     7021 tccggcaacc tcctctctgc catcctcatc caaaagcaga ttcaggcgtt gtcgtgccga
     7081 caacggattg tggtggttgc atttcggaca aacctgattg ttctgctgca actcggtcga
     7141 ataaacggtt gccgaacaag acggacattt gtgccacaaa ccctcgggaa cattggaaga
     7201 accgtctttg ctgcggtttt tgattttggg tggcaggatt ttatctaacc agctcatgga
     7261 caactcctaa aatatttaga atgcgtgaca gaatcagcgc acggcatcct tcaactcttt
     7321 gaccaaagca ccgacggccg cagcctcgtt gcccgcattg ttttcaatct ctttcacaat
     7381 ccggctgccg acaatgaccg cgtcggcaac ccggccgatt ttgcgcgcgc tttccgcatt
     7441 gctgatgccg aaaccgacac cgatgggaat atcgatatac tgatgcaaat actctatttt
     7501 acgcgaaacc tcatccgtat ccaaacttgc cgcgcccgtt acgcccttga gcgaaacata
     7561 atagacaaat ccgcctgcca gctcggcaat ggttttaata cggtcttccg tcgtcgtcgg
     7621 cgcaatcagg aaaatacagt cgaccccgtt atccttcagc tcgcgataga gcggatcgat
     7681 ggtttcgaca ggggaatcca ccgtcaacac gccgtccaca cccgccattg cggcttcctg
     7741 agcaaactcc cgataaccca tcttatgtac agggttcaaa tatcccatca aaacaaccgg
     7801 cgtttgcgtg tcggtttcac ggaattttct gacgacatcc aagacatcgc gcagcgaaat
     7861 cccgtttgcc aacgcccgct ccgccgcacg ctgaataacc ggtccatccg ccatcggatc
     7921 ggaaaacggc acgcccaact ccaaaatatc cgcaccgttt gcaaccatgc cgtgcatcaa
     7981 tgccaaagtt gtccgaatat cggggtcgcc caccgtaata tagggaatca atgcctttcc
     8041 gccatccaaa gcggcaaaag cctgccggat tctgctcatt tgtctcttcc atcctatcgg
     8101 caaatatgcc ataaaacaca atcagtaaat ttttcccagt ataacatttg cggtacggat
     8161 aggcgaatac ggcaaacaga aaccacgcaa atcagagccg ccgtttgaac gaggcggaaa
     8221 taaacggcaa gcatctcgga ttatagtgga ttaacaaaaa tcaggacaag gcggcgaagc
     8281 cgcagacagt acaaatagta cggagccgat tcacttggtg ctccagcacc ttagagaatc
     8341 gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataacaa
     8401 aattcagaat caagacaggg caataccata ctggttttgc accaacccgc tataaaattg
     8461 cataaaaaaa atagacgctg taaaacacag cgtctatttt ttatctggcg gagtaagagg
     8521 gattcgaacc ctcgatgcag gttgacccca catgcttcct tagcagggaa gtgccttcag
     8581 cctctcagcc attactccta aggaagctgt aactataatc aacttttccg ataccgtcaa
     8641 ggaaaaatgg aaagcaaata ttaatctttt gaaataaaat aagatttacc ctgattatac
     8701 ccaatcaggc acttcttttc ttcctgaatg gaaatattcc tgactgccac atcctcaagc
     8761 aaaatgcttt tgtgcatcct gtttgatttc ctgaaaccac gcgtcttcag gaaaacgtgc
     8821 aagcgcggca tccaatgttg acagtaggtt ttgcgacagg gctttggtca ggtattcctc
     8881 ctcgttggct tctgccgctt tgtcgggctg ttgtgccaaa ccgtcggcac gggctttgtg
     8941 tccgtgtgcc aaatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
     9001 gtacagatag tacgacaagg cgaggcaacg ccgtactggt ttaaatttaa tccactataa
     9061 ttgagcgcgg taattttttg cgcccaaagc tgcggtatgt cgggattgtc ttcaatcatg
     9121 acggaaagga tggatacggc ggtcgccagc ctgccgccgt tcattctaag gtcgagggaa
     9181 cggttgggcg gcagggtgct gagtgtttct gccattctga accaaaattc gccactggct
     9241 tcgtcgggtg cgttcaattt gagcatttca tagtgaacct cctgatcgtc gttttgcacg
     9301 agaccccata cttcagcaag gattttctgt tcttcccggc tcaagtgttg ccatttttca
     9361 atatgttgct gcatatcttt tccctttgaa tctattttat atagtctgaa atacagcgcg
     9421 aatcctgtcc aaatcctcct gcgtatctac gccggcggca ggagcttctt tggcggtttc
     9481 gacggcaatc gggtagccgt gccacaggac acgcagttgc tccagcgatt caatggtttc
     9541 cagcggcgaa actctcattt cggtgtagcg ttgcaggaag ccgacgcggt aggcgtagat
     9601 gccgatatgg cgcaggacgg cggtttcaaa cggcatttcg cgttttcctg cacgcatcgc
     9661 atcgcgcgga tagggaatcg gggcgcggct gaagtagatg gcgttgcggt ttttgtcgag
     9721 gacaactttg acggcattgg gattcatcaa ttcgtcgaaa tcgtgcaatt cgtgggcggc
     9781 ggtcgccatt tggacgttgt tttcgacgag tacttcggcg gtgcggtcga tgagttcagg
     9841 tgcaatcagc ggctcgtcgc cctgtacgtt gacaaccacc agatgcggcg gcagcttcag
     9901 cgcggcagag gcttcggcaa ggcgcgtcgt gccgctttcg tgccggtttg aagtcatgac
     9961 gacttcgacg ccgtgcgcct gacaggccgt ctgaatatcg ggatggtcgg tggcaacgac
    10021 gacgcgcaca gctttacttt ttgccgcctg ttcggcaacg cgcacgacca tcggtttgcc
    10081 gtggatgtcc gccaaggctt ttccgggcag gcgcgacgaa tccagccgcg ccggaatcaa
    10141 tacgacgaat tcggtcatgc ttcgagttcc tcttcgccca acggacgcgc ttcgttttcc
    10201 agcatatagg gaatgccgtc tttaatcgga taggcaagct tcgcctgacg gctccacaat
    10261 tcctgtttgt cctgatgata ttccagcctg cctttggtaa cggggcagac gaggatgtct
    10321 aagaattttt tttccatagg gttgctttcg ggtttgatgg ttcaatcaat cggatgccct
    10381 cttcaatatt tcagacggct atcatttatt tctgatgcct tttcccaacc tgtccggctt
    10441 gtttacagcc cgcatcggga tttttaccgc cgcagatgat gcgccgcaac tcttccgtaa
    10501 acgcgggcag atgatgtcgc gcgattttcc ctgacaatgc agtccaagat actgctattt
    10561 cactcatcga tttcttgatt aagcgcggat ttttggcaac gacggactga ccgacaggcg
    10621 aaccgtaaaa ggcaatcatg ccgtcaattt cttcttgcgt gtatatctca cgggcattct
    10681 tcaataaagt attacggaca gcctgtttca cttcgggcgt aatcaaatct ttcaaaacat
    10741 tctcacgata acggttaaag gcttctgccg cctgatcttt tttcgcttcc ggcatttcgg
    10801 caagggcttt gtccgcatac ggtttaaatc cggcattaaa gccctcaatc atattttttt
    10861 ctatatcccg gtcaaaattc tgcgtatcca gccaacgcgc caacgacgcg tcgctgggcg
    10921 gggcggcaaa agcgttggtg cacaatgcca gcgtggcgaa gggcaataac aaggttttca
    10981 gtttaatatt caatccttta acgaatgatt aatgcagata aaaaatacga ccgattttct
    11041 cattcctgtc caagtggaaa tccggagatt tgggatttct gaagcctcct aggcattcct
    11101 gaaaattcga gacttagatt cccgcctgcg cgggaatgac ggcgcgaggc gttcttattc
    11161 aaatccgtaa tcactccgcc caaaccgtgc tttcagacgg ccttcggtac atcttccaac
    11221 cgctccaaca caaacgccgc caaatcaggt tcgattatcg cacaaacggg caacacccaa
    11281 acattatcgg tgcaaatgcc gtctgaaaat ttgaccgcat ctttctccgt aataatgacc
    11341 gcgtccgcat cgggcaaatc tgccgccgaa atgtcggcgt ggtcgggcag cgcgacggtt
    11401 cgcttcacgg taatgcccat attccgcaac gaatcgaaaa aacgcgccgg cttggcaata
    11461 cctgccacgg cgacgacggt ttgatttttc agacggcctg tgtccagtat ttcggacgga
    11521 ttgttcaaac ggtaaattcg tcccgcttcg atacggctgt gaaacatatt ttcagacggc
    11581 ctaaacagcg catccgcctt gccgccgctg acgacgaccg tatccaccga atccagccgc
    11641 gacaaaggtt cgcgcaaatt gccgttgggc agcaaatcca aatccgtgcg ccccgtatcc
    11701 gccgccggaa acaccgcgat ttccacatct cgccgcaggg cgtaatgctg caaaccgtcg
    11761 tccgccacaa tcagtccgat gtcgggatgc gccgccagca acgccctgcc tgcctctgca
    11821 cggctgctgc ccaccgccgt cggcgcaccg gttttgctga acagcagcaa aggctcgtcg
    11881 cccgcatctt ccgcacggct cgcggcattc aatacatgaa ccgccttgct cttgcgcccg
    11941 tagcctcggc tgatgatgcc gaccttgaca cctttttcct gcaaacccga caccagcgcg
    12001 gcggcaatcg gcgttttccc cgtcccgccc gcgtggatgt tgccgaccac gaccacaggc
    12061 acgggcagct tttcgctttg ccgttttccc gataaaaaat ccgtccgccg ttttgccgca
    12121 attttggcaa acagcccgga gagcggctta agcagaaaag acaaaaccgg ataaggtttt
    12181 tgccaatgcc gttcgataac ggtatgaaat ttgaaacaat tcggcatatt cgatacaaat
    12241 cctcgaagca aaatcagccc ggggcattaa tcatgcgtac ccgatacaga cccacctata
    12301 aacaacgcga agaccaaacc gataagcgaa ccaatcaggt aaataagcaa ctgcgcttta
    12361 cagaaattgg cgcggttcgg gttgcctctg ccgaacaccc aaacaaaaat tgaccaaagg
    12421 aatcatgaaa acaatcatcg tcaacaccca ctgccctgtc gtcatgacag gggcggtttg
    12481 ttttgaagtc tcgggcatat cgccgatatg ataacggaca gtctccatct ctttcatttt
    12541 ccagtatctt tcatttatca atctgacagc aaaatgccgc ctgaaaacag ttcagacggc
    12601 attttaaacc gcaaacggct tacagcttcc attcgcccag cttggcggcg taggcttcaa
    12661 agtcggcaat cggacgggtt gccacgccgc tttccatcgc tgctttggcg gcagccgtag
    12721 caacgcgcgg cagcaggcgg gaatcgaacg gagtcggaat caggtattcc gcgccgaatt
    12781 caaatttctt accgtaagcg gcaaccactt cttcggttac ttcttccatc gccaaatccg
    12841 ccaaagcata cacgcaggag cgtttcattt cttcgttgat ggtcgtcgcg ccgacatcca
    12901 acgcgccacg gaagatgaat gggaagcaca atacgttgtt cacttggttc gggaagtcgg
    12961 aacggccggt accgataacc acgtccggac gggtttcttt tgccagcggc ggcaggattt
    13021 ccggattcgg gttggccatg gcgaacacga tgggtttttc gttcatcgtg ttcaacattt
    13081 cgggggtcag caggttcgcg ccggagaggc ccaagaagat gtctttacct ttgacggcat
    13141 cgacaagtac gcgctggcca ttgtcttcaa cggcgtagaa ttttttggat tcgtccatgc
    13201 gatctttgtc ttcgcgggtt tggtaaatca cgcctttgga gtcgcaaacg gtcacgtttt
    13261 cgcgtttcaa gcccaaatcc agcaattggt tcaagcaggc aatcgcggcc gcacctgcgc
    13321 cggaacacac caaagtcgct tcttcgattt tacggccggt aaaacgcagg gcgttcaata
    13381 cggcagcggc ggtaatgatg gccgtgccgt gctggtcgtc gtggaatacg gggattttgc
    13441 agcgtttgcg taattcgcgt tcgatgtaga aacactcggg tgctttgatg tcttcgaggt
    13501 tgatgccgcc gaaggtcggc tctaaagcgg cgatgatgtc cacgagtttt tgcgggtctt
    13561 tttcatcgat ttcgatgtcg aacacgtcca caccggcgaa ttttttgaac aatacgcctt
    13621 taccttccat cacgggtttg cccgccagcg cgccgatgtc gcccaagccc aaaacggccg
    13681 taccgttgga aatgacggcg accaagttgc ctttggcggt gtatttgtag gcattttgcg
    13741 gatcggcatg gatttccata caaggagcgg ctacgcccgg agagtacgcc aacgccaggt
    13801 ctttagacgt ggccagagat ttggtcgggg taacggagat tttgcccgga acgggtaatt
    13861 cgtggaactt gagggcggct tctttcaatg agttttccat gtgtcaatcc tgttgcgaga
    13921 aaaagatttg aatcggactg tccgaaacgc tttcgggtca tcatgttacg ggcggttcgt
    13981 taatattgcc gacgcatcct gccttgtaac cgagcgtaat tctatcatgc cgaaagtttg
    14041 gcgcataccg ccattttcac ctgcggcact gccgtttgat tttcggcgcg ggtggatgcg
    14101 cttgtttcaa tatttgccgc attaacagcc gaaatgtccg tccaacactt tttctatgtt
    14161 gtttctgact tcatccaagc tgcggttact gtcgataatg gcgtaccgtt cgggacaggc
    14221 ggcggcacgg ttcagataaa cgctgcgcac acgcatgaag aaatctgcct gttcctgctc
    14281 gaaccggtct ttctcgcgcg actgtccgat acgcgccatc gacacttcca gcggcacatc
    14341 caacagcagg gtcaaatcgg ggcgcaaacc gccctgcacc caatgttcca aaatttcaat
    14401 gtcttcagac ggcatccccc gcccgccgcc ctgataggcg aaggttgcgt cggtaaaacg
    14461 gtcggacacg acatggatgc cgtctgaaag tgcaggcaat atcacgtctt caatatgctg
    14521 cattctggcg gcgaacatca tcagtgtttc cgcacgcaaa ccggctttgg tttcagggtt
    14581 gagcaggatt tcgcgcaagg cctcaccgac cggcgttccg cccggttcgc gcgtgaacag
    14641 cacgggcagc cccctccgtt caaaccatgc cttgatgacg gcaaggttgg tggatttgcc
    14701 ggcaccgtct atgccgtcca aagtgatgaa ttgcggtttc atgcttgcag ttccggtaag
    14761 ggtatgccgt ccgaaaacac aaacttttca gacggcatgg tttatttttt caaaatatat
    14821 ttgcgaacgg cggcgttgtg ttcggtcaaa tcatggctga actggctcaa gcccgtaccg
    14881 tccattttgg acacgaaata caggtatttt tcaccggacg gatgggcggc ggcatcgagt
    14941 gccgccttgc cgggcagcgc gatcggggtt ggcggcagac cgccgcgcgt gtaggtgttg
    15001 tacggcgtgt cgcggcgcag gtcggcttta cggattttgc ccttgtatgc cgcacccatg
    15061 ccgtaaatca cggacgggtc ggtttgcagg cgcataccga ttttcaggcg gttgacgaag
    15121 acggaagcga catggtcgcg gtcggcttca tgccctgttt ccttttcgat caggctcgcc
    15181 ataatcagca tttcataagg gtttttataa ggcagcccgt cctgcctgct ttcccatgcc
    15241 tcattcagtc ggcgttgcat cgccttgtag gcgatttggt aaatccgtaa atcgctgccg
    15301 cccgcatcga tttcgtagct gtcggggaaa aactgccctt caggattgcc gctgaaggca
    15361 tcaggggcaa cttccgccat cagtttttca ttgctccagc ctttggtgtc gtgttcgatg
    15421 tcgggcgttg cgtcgatgac tttcctcata tgcgaaaaac gcgaaccttc gataatctgc
    15481 acggtaacgg aatccggcct gccgccgcgc attttctgca agatatccca agcagacact
    15541 tccgaaggca gtctgtacgt ccccgtatac agcctgttgt gcacacccaa aacgtaggcc
    15601 gccgccgtca aaacatgcct gctgaacacg atgcggtctt cggcaagttt cctgccgacc
    15661 gacgaaatac cctggttttt ggcaatttta atcctgtatg ccctgccgtt gtctttaggg
    15721 acgaaaagca gcgcggcgaa aacggctgcc gatacggtca aaaaaacggc agaccatttc
    15781 aacaattttc tcaacatggt aggattccca gcattcggaa tacgtcttaa aattcgggcg
    15841 acagtataac ccaaattgcc cgccggacag gaagaacatc atggacaatc acgccgaagc
    15901 acactggcaa aacggctggc ttcaaagcat acgccatacc ccgtcgccca atttcagccc
    15961 gagggaaacg ggggaaacgg tttccctgat cgtgttgcac aacatttcgc tgccgccgtt
    16021 cgaatatggt acggatgcgg tggaaaagct gtttgccaac cggctcgacc ccgacggaca
    16081 tccgttcttc agcctgatac acactttgcg cgtatccagc cattttttaa tcaaacgcga
    16141 cggcgaaacg gtacagttcg tttcctgcga caatatggca taccatgccg gcgtatcctc
    16201 gtttcgcgga cgggaaaaat gcaacgcatt ttccatcggc atcgaactgg aaggctgcga
    16261 tttcgaaccc tttaccgaag cgcaataccg ttcgctcgaa gccttgttgg acgcaatctg
    16321 ccgccgctac cccatcacgg cggtaacggg gcaccaagac atcgcgcccg gccgcaaaac
    16381 cgaccccggt cattttttcg actggcggcg gatacggggg aaagggtttc ccgtggacag
    16441 aaatgccgtc tgaaaaccac agattttaga tggcatttcg gaaaatttcc gcttatttcg
    16501 acacactgct gtcagaccct gcaattttcg ttataattca aacgttataa ccggccgttc
    16561 cgtcagactg ccggaaacgc aagcccgatt taaccgcagt acccgcactt tgcgcatcct
    16621 gcatcccgaa atttaaagtg attgccatga tttacatcgt actgttcctc gccgccgtcc
    16681 tcgccgttgt cgcctacaat atgtatcagg aaaaccaata ccgcaaaaaa gtgtgcgacc
    16741 agttcggaca ctccgacaaa gatgccctgc tcaacagcaa aaccagccat gtccgcgacg
    16801 gcaaaccgtc cggcgggtca gtcatgatgc cgaaacccca accggcggtc aaaaaaacgg
    16861 caaaacccca agaccccgcc atgcgcaacc tgcaagaaca ggatgccgtc tacatcgcca
    16921 agcagaaaca ggcaaaagcc tccccgttca aaaccgaaat cgaaaccgcc ttggaagaaa
    16981 gcggcattat cggcaactcc gcccacaccg tttccgaacc ccaaaccgga cattccgcac
    17041 cgaaacctgc cgacgcgccg gcaaaacctg cacccgttcc gcaaacacct gcaaaaccgc
    17101 tgattacgct caaagaactg tcaaaagtcg aattaccctg gtttgacgtg cgcttcgact
    17161 tcatctccta tatcgcgctg accgaagcca aagaactgca cgcactgccg cgcctttcca
    17221 accgctgccg ctaccagatt gtcggctgca ccatggacga ccatttccag attgccgaac
    17281 ccatcccggg catccgctat caggcattta tcgtgggtat tcaggcagtc agccgcaacg
    17341 gacttgcctc gcaggaagaa ctctccgcat tcaaccgcca ggtggacgca ttcgcacaaa
    17401 gcatgggcgg tcagacgctg cacaccgacc ttgccgcctt tatcgaagtg gcttccgcac
    17461 tggacgcatt ctgcgcgcgc gtcgaccaga ccatcgccat ccatttggtt tccccgacca
    17521 gcatcagcgg cgtagaactg cgttccgccg taacgggcgt gggtttcgtc ctggaagacg
    17581 acggcgcgtt ccactatacc gacacgtcgg gctcgaccat gttctccatc tgctcgctca
    17641 acaacgagcc gtttaccaat gcccttttgg acaaccaatc ctataaaggc ttcagtatgc
    17701 tgctcgatat tccgcactct ccggcaggcg aaaaaacctt cgacgatttg tttatggatt
    17761 tggcggtacg cctgtccggc cagttgaacc tgaatctggt caacgacaaa atggaagaag
    17821 tttcaaccca atggctcaaa gacgtacgca cttatgtatt ggctcgtcag tccgagatgc
    17881 tcaaagtcgg tatcgaaccg ggcggcaaaa ccgcattgcg cctgttctcc taaaatcgaa
    17941 tgcaatcaaa gcggatatgt cgttacggca tatccgcttt ttttcgggta aaattcagct
    18001 gttttcaacc tatcattttt cagacgacct ttgcccgttt catcccgtat cagacaaagg
    18061 tcgtctgaaa accttctctc atgaatccaa actcaaaaca taacactaac tttaccaatc
    18121 ttcttaaacc gtctgatagt gatataaaac aatttgccgc acaacacatc cgccacctca
    18181 ccgacctgct caaccgctat gcctacgaat actacaccct cgacgcgccc agcgtacccg
    18241 atgccgaata cgacaaatta ttccgcgagc tcgaagcgtt ggaattaaac catcccgagc
    18301 tcaaactgcc cgacagtccg acccagcgcg tcggcggcga gcctttagcg ggatttgccg
    18361 aagtgcgcca cgaagtgccg atgctgtcgc tgaccaacgc tttttccccg caagatgaaa
    18421 acggcgtgtt cgaccatgcc gaaatgtacg ctttcgacca acgcgtgcgc gacggtttgg
    18481 acggcggcaa acccgaatac gttatcgaac ccaaattcga cggcctcgcc atcagcctgc
    18541 tctaccgcga cggcgtattg gtacaggcgg caacgcgcgg cgacggcaca acgggcgaag
    18601 acgttaccca aaacatcaaa accgtcgcca acatccccct gcggctgcac ggcgaaaata
    18661 cgcccgaact catcgaagta cgcggcgaag tactgatgca aaaagccgat tttgccgccc
    18721 tcaataaaag acaagccgaa aacgggcaaa aaccctttgc caatccgcgc aacgccgctg
    18781 ccggcagcct gcgccaactc gattcgcgca tcactgcgca acgcaaactg cactttttcc
    18841 cctactccgt cgcccgccag caaggcggtt tcgtcgcgga agaacacatc caagaactcg
    18901 cctatttcca agcactcggc ttcagtctgc caaatggcaa tttcggctgt ttcaaaaata
    18961 tcgacgaagt attggcgttt tacgaacaaa tgcagcaaaa acgccccgaa ctgccctacg
    19021 aaatcgacgg tatggtggtc aaagtcaaca gcttggcgca acagcgcgaa ctcggcttca
    19081 tctcacgcgc gccacgctgg gcgattgccc acaaattccc tgccgaagaa gccctgacca
    19141 ttgtcgaagc gattgacgtg caaatcggca ggacgggcgc ggtaacgccg gttgcccgat
    19201 tacaacccgt attcgtcggc ggcgtaaccg ttaccaacgc caccctgcac aatcaggacg
    19261 aagtttcccg aaaagacgtg cgcgtcggcg ataccgtcgt cgtgcgccgc gctggagacg
    19321 tgattcccga agtcgtgcgt gtgatttttg aacgccgccc gatgcaggaa accgccgttg
    19381 ctgtttcaga cggcatcggg catcggcagg acgatttgtt tgccgaaaca ccgtccgcta
    19441 atcaaaccca atccgttccg ctccacaagc cctaccgcct gccgacccat tgccccatct
    19501 gccgcagcga aatcgaacgc gaagaaggcg aagccgtcgc ccgatgcagc ggcggtatgc
    19561 tttgtcaggc acaacgcgcg caaggcttaa tccacttcgc ctcgcgcaaa gccatggaca
    19621 tcgacggctt gggtgaaaaa caaatcgaac agcttgtcgc ccaagacctc gtccgccact
    19681 tcgccgacct ctaccgcctc gatattccga ccctgcaaaa aatgaaagaa acgacggaca
    19741 aaaccgttgc cgaatcggat caaacgccgt ctgaaggcag ttcggtcggc gcgtcgggca
    19801 aacataaaaa acaaccggtc aagtgggcgg aaaacatcct cgggggcata gaatccggca
    19861 aaacgcccga actcgcgcgc ttcctgttcg cgctcggcat ccgccacgtc ggcgaacgca
    19921 ccgccaaaac gctggcgcag gcattcggca cattggaacg cgtccgccgc gcccccgaac
    19981 ccgtcctcgc ctgcctgccc gacatcggta cggtagtggc gcattccatt gcccacttct
    20041 tcgcccaagc cgaacagcag gcgatgatag acgaactgct cgccgcaggc gttgccccgc
    20101 aaacccaagc cgttaccatc ccgcccgccc gccacgccga accgcaacgc tggatcgccc
    20161 gcctgcccga cttcaaaatt agcgaaaaca aagcccaagc cttatgggaa ctcgctggta
    20221 aaaacataga aggtctacaa aacgacaaag ccctccccgc cgactggcaa gcatggcgca
    20281 gcgaaccaca aaacgccgcc ctactcgaaa acctgaaaac cttcttcgcg caaatgccgt
    20341 ctgaagacga ggcggcgcaa ggttcagacg gcatcaataa agccgtagca ggcaaaacct
    20401 ttgtattaac cggcaccctg cccacgctca aacgcgacca agcccaatcc ctaatcgaag
    20461 ccgcaggcgg caaagtttcc ggcagcgtgt ccaaaaaaac cgactacgtc gtcgccggag
    20521 aagccgcggg cagcaagctg gaaaaagcca atgccttggg cgtttccgtc ctcagcgaag
    20581 ccgaactgct taccctgctc ggctgaacct atgccgtctg aaccgccttc agacggcatt
    20641 gtccactttt ccgaaccaca ccatgcacac catttccttt ccaaaccgca cccgactcac
    20701 cgccctgccg cccctgtcgc tttacatcca catcccgtgg tgcatcaaaa aatgcccgta
    20761 ttgcgacttt aattcccaca gcctgaaaaa cggattgccc gaagccgcct atatcgacgc
    20821 gctgctgacc gacttgcagc tcgaattgcc caatatttgg ggcaggccgg tggaaacgat
    20881 ctttttcggc ggcggtacgc ccagcctgtt tcaggcggaa tcgattgacc gtttattgag
    20941 cggcgtgcgt tcgctgttgc gcttgcagcc ggaagcggaa attaccttgg aagccaatcc
    21001 ggggacattt gaaatcgaga agtttcaagg atttaaagac gcaggcatca cgcggctttc
    21061 tattggtgta cagagtttca acgacgatat gctctcaagg ctggggcgcg tccacaacgg
    21121 caaggaagcc ttaaccgcta tcgataccgc cttgaaatta tttgataaag tcaatatcga
    21181 cttgatgtat gccctgccga accaaaccgt tcagacggca ttggacgacg tacaaaccgc
    21241 tatcgctacg ggcgcgaccc acatcagcgc gtatcatctg acgatggaac cgaacacgcc
    21301 gttcggtcat acgccgccga aaggtttgcc gcaagacgaa gccgccctcg acatcgaaga
    21361 cgcggtacac ggcgcgctgg aaagcgcggg ttttgtccac tacgaaacat cggcttttgc
    21421 gaaaccagcc atgcagtgcc gccacaattt gaactactgg cagttcggcg attatttagg
    21481 cataggcgcg ggcgcgcacg gcaaaatttc ctatcccgac cgcatcgagc gcaccgtccg
    21541 ccgccgccac cccaacgact acctcgcctt aatgcaaaac cgaccgagcg aagccgtcga
    21601 acgcaaaacc gtcgccgccg aagatttgcc gtttgagttc atgatgaacg ccctgcgcct
    21661 gaccgacggc gtacccgccg cgatgttgca ggaacgcacg ggcgtaccga gtgccaaaat
    21721 catggcgcaa atcgaaacag caaggcaaaa aggcctgctg gaaaccgacc ccgccgtatt
    21781 ccgcccgacc gaaaaaggac gcttgttttt aaacgatttg ctgcaatg
//
LOCUS       DAXHMX010000015        21718 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHMX010000015 DAXHMX010000000
VERSION     DAXHMX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.015"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(92..783)
                     /locus_tag="ACFM4S_000437"
                     /pseudo
     CDS             complement(92..783)
                     /locus_tag="ACFM4S_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1042..1761)
                     /locus_tag="ACFM4S_000438"
     CDS             complement(1042..1761)
                     /locus_tag="ACFM4S_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050181.1"
                     /translation="MLAIYSINILIYNEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKH
                     KCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSISYINTERNQSEFANPIYVQ
                     TSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQAEMKETQGEFVPIIAAAAF
                     GGALGTWGYHGANLYNHGKLGTAQGAATAAGIGAATGVAATGLAAAAGGGLAGNLAWR
                     PGIHALGFGANAANNAVNSRK"
     gene            complement(1888..2046)
                     /locus_tag="ACFM4S_000439"
     CDS             complement(1888..2046)
                     /locus_tag="ACFM4S_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050182.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2043..2885)
                     /locus_tag="ACFM4S_000440"
     CDS             complement(2043..2885)
                     /locus_tag="ACFM4S_000440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6050183.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPTDERVLLAQAIEEKHAAEKAQEQTVSGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3121..4041
                     /gene="pyrB"
                     /locus_tag="ACFM4S_000441"
     CDS             3121..4041
                     /gene="pyrB"
                     /locus_tag="ACFM4S_000441"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6050184.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4051..4509
                     /gene="pyrI"
                     /locus_tag="ACFM4S_000442"
     CDS             4051..4509
                     /gene="pyrI"
                     /locus_tag="ACFM4S_000442"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6050185.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4574..5239
                     /locus_tag="ACFM4S_000443"
     CDS             4574..5239
                     /locus_tag="ACFM4S_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050186.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5302..6519)
                     /locus_tag="ACFM4S_000444"
     CDS             complement(5302..6519)
                     /locus_tag="ACFM4S_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6050187.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWSRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAGE
                     RLKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6703..7206
                     /gene="def"
                     /locus_tag="ACFM4S_000445"
     CDS             6703..7206
                     /gene="def"
                     /locus_tag="ACFM4S_000445"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6050188.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            7293..8219
                     /gene="fmt"
                     /locus_tag="ACFM4S_000446"
     CDS             7293..8219
                     /gene="fmt"
                     /locus_tag="ACFM4S_000446"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6050189.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8298..9557
                     /gene="rsmB"
                     /locus_tag="ACFM4S_000447"
     CDS             8298..9557
                     /gene="rsmB"
                     /locus_tag="ACFM4S_000447"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6050190.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASTACADAQDLSAWYDGKPFDAVLADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQVLKSGGRMLIATCSVFVEENDGQLQKFLNRHA
                     DAELIESRVLLPNKHQDGFYYALIQKH"
     gene            9523..10122
                     /locus_tag="ACFM4S_000448"
     CDS             9523..10122
                     /locus_tag="ACFM4S_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6050191.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10122..12242
                     /locus_tag="ACFM4S_000449"
     CDS             10122..12242
                     /locus_tag="ACFM4S_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6050192.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATILPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEVGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            12235..13512
                     /locus_tag="ACFM4S_000450"
     CDS             12235..13512
                     /locus_tag="ACFM4S_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6050193.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEVGSPFETVARYFHKNGTPWVSPARVEYLIDMPTELLQKAEGGVLYVGDIAQYSR
                     NIQTGIAFIIGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13603..14790
                     /gene="dprA"
                     /locus_tag="ACFM4S_000451"
     CDS             13603..14790
                     /gene="dprA"
                     /locus_tag="ACFM4S_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6050194.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGSRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14816..15277
                     /locus_tag="ACFM4S_000452"
     CDS             14816..15277
                     /locus_tag="ACFM4S_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6050195.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15349..17655
                     /gene="topA"
                     /locus_tag="ACFM4S_000453"
     CDS             15349..17655
                     /gene="topA"
                     /locus_tag="ACFM4S_000453"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6050196.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAETCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17733..18002)
                     /locus_tag="ACFM4S_000454"
     CDS             complement(17733..18002)
                     /locus_tag="ACFM4S_000454"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFC6050197.1"
                     /translation="MIASSEPFPCSACGLCCQKVNLSEQTIYLDRGDGTCRHFDDSTR
                     LCTIYETRPLVCRVEDYYHHYLSEQMSWQDFIQINIEVCKQFQSE"
     gene            complement(17999..18418)
                     /locus_tag="ACFM4S_000455"
     CDS             complement(17999..18418)
                     /locus_tag="ACFM4S_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050198.1"
                     /translation="MGKIQILNKLPTKNPATIPFDAASNSFFRILEARNEYKKIAAQE
                     KTKREVIGAWRDVRLAELKNQQEILRLYLENTFKERRKMIDGLFEALDKGMEAGNIHV
                     IDTAINGIVSIAKDSPLKQVDNLIAALKNDNVKVIDF"
     gene            complement(18418..19365)
                     /locus_tag="ACFM4S_000456"
     CDS             complement(18418..19365)
                     /locus_tag="ACFM4S_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050199.1"
                     /translation="MAIPVLLWGAAAALAATGIVKGAAALSDIDDAKEIGERAERRYK
                     RRKRELNSTKDETNLAFEELGQFKVDIFNNQIRHIVQVLNNTSNKHAKSELSGFNSTF
                     DIAELKEMETMVQTSLELSTNLGSSAITGALAGFGVYGAVPMIATASTGTAISTLSGA
                     AATNATLAWLGGGSLAAGGFGMAGGMVALGGIVAGPALAIGGFMLASKAEEALTEARA
                     YSAEIDEAIDEMDLITEGLEGLKENAREIRHTLEEMVKRFEKVRVDHADHPDFHTMLV
                     IGKAIKNLLNTPIMEEDGSPVANIRHQCEGCLNIELGTV"
     gene            19522..19899
                     /locus_tag="ACFM4S_000457"
     CDS             19522..19899
                     /locus_tag="ACFM4S_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6050200.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19988..20557
                     /gene="rsmD"
                     /locus_tag="ACFM4S_000458"
     CDS             19988..20557
                     /gene="rsmD"
                     /locus_tag="ACFM4S_000458"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6050201.1"
                     /translation="MAAGKHTKHSNQVRIIGGQCRGRKLSFASANGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGRL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20680..20931
                     /locus_tag="ACFM4S_000459"
     CDS             20680..20931
                     /locus_tag="ACFM4S_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6050202.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            21028..21111
                     /locus_tag="ACFM4S_000460"
     tRNA            21028..21111
                     /locus_tag="ACFM4S_000460"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21062..21064,aa:Tyr,seq:gta)
     gene            21142..21215
                     /locus_tag="ACFM4S_000461"
     tRNA            21142..21215
                     /locus_tag="ACFM4S_000461"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21174..21176,aa:Gly,seq:tcc)
     gene            21225..21299
                     /locus_tag="ACFM4S_000462"
     tRNA            21225..21299
                     /locus_tag="ACFM4S_000462"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21257..21259,aa:Thr,seq:ggt)
     gene            21348..>21718
                     /locus_tag="ACFM4S_000463"
     CDS             21348..>21718
                     /locus_tag="ACFM4S_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6050203.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLAR"
BASE COUNT         5417 a         5573 c         5864 g         4864 t
ORIGIN      
        1 aagtgaatgg gtttcgtact atttgtactg tctgcggctt cgtcgccttg tcctgattac
       61 tatataaaat aaatgggcaa aaatcggttt attatcgttt ttgccgcatt tggatttgtt
      121 ctaccgtaaa acgtgtttga cgaacgggat tcttattaaa aaacatctga tttctaacaa
      181 aatcagtatt ttttggcacg atggctaaaa tttttccttc catttcgcca tcacgtgttt
      241 tccatgcgct caagaattgt gatttgctca ttgagacgtg ccccagcgat ggatcagcca
      301 gcaaaacagt ttctccgtta ataccgttca ataccgaaaa atggttgttt ttacggtatt
      361 ttaaatacac aattacagga atttttagtt gtaccaactg ttcaaatggc aaagcataac
      421 cttgtgcttc aaaacccagt tcgggcatta tgcgttgcat atcatcaaaa gaagcacgca
      481 tttgggtttt atccattttg tctaagattt ccgcttcaga ataatgtctg ccataaaaat
      541 tattcagtaa cgtggcaatc gaagccgcgc cgcaagaaaa atccaaatct tgttttacta
      601 tgccggaatc tcgccgtgct ttccaacttc gtacatggat gttttggtaa gaagcggggg
      661 gtcaacaaac ataggccaag caaaaactat atttggggcg aaaccaatca aagccgcata
      721 atttatcaat ttataaagat tttttatcat aatatgtata cgcggaataa acgcatgaaa
      781 cataaaaaaa taaaatcata tgcaatttta ttgcatgaat aaatatgaat aaaatagata
      841 atgatgggag taaatacgcc atgtattttg gaagtttaaa tttattaata ataaaataat
      901 ttatcgtagc gcaaataaat cccaaaattg gaacgattag aaaaaaaatt aatacaccca
      961 tcatcatgca aactctatat taagaaatag ctaactaatg ctattgtgga aattagttag
     1021 ctaactaaaa gttattaatg attattttcg agaattgact gcattattag cagcattggc
     1081 accaaaacct agtgcatgaa tacccggtct ccatgccaaa ttcccagcca atccgcctcc
     1141 ggcagcagca gccagccctg ttgccgctac tcctgttgcc gcaccgattc ctgccgcagt
     1201 agccgcgcct tgcgcagttc ctaatttacc atgattatac aaattagcac catgataccc
     1261 ccatgtacct aatgcaccgc caaaagcagc ggctgcaata atgggaacaa attcaccttg
     1321 tgtttctttc atttcagcct gtgataattg aattgctttc acattttggc tgtcaaaaac
     1381 ttggctgtct aaattttgcg ccattacagg tgtaatcatc atagccatta cagttgcaat
     1441 tttcgttgcg ctggtttgca cataaatagg attagcaaat tcgctttgat tgcgttcagt
     1501 gttgatgtag ctaatactgc tttctagttt gaatttaccc ttgtcagtca ataattcttc
     1561 caaacttaaa ggtaagtcag cataagcaat ttggctggca acagtcaaaa taaaatctat
     1621 tagacatttg tgtttttgca tcatttcgtt tgattttcta gtttttgaga atgatacaaa
     1681 gttttttaca aagtaaagag tcactctgaa aaacattttt tcattataaa tcaaaatatt
     1741 gatagaataa atagcgagca tcgattcacg gtgtacttta gtgcaaagcg tacaacgcga
     1801 gcctgaacca ccagccgcaa caggaaaaga aagccgatag agtgcaatgc ttgccaacgt
     1861 gcaagcgagc ttgcaagaac gcttggctca acgagagcag gcaagacaga aagcagaaag
     1921 caggatagga gcggtaacgc aaaggtctcg ggctttgatt tcgccgtaaa ccctgctgcc
     1981 gccttgtccg gaaagggtgc aggcggcgag tgccgacagg gtgcagatgg ggaggggggt
     2041 tttcatttgg ggtcgcaacg gaagtggtat gcgcagattt caaaaccgtt tttgaaatac
     2101 aggcggtgcg cgtcggcacg gtcgtggttg acgtggacgt tgaggtggat tttggttacc
     2161 cctgtttccg cgccgatttt gcggacttct tccaaaaggc gcgaggcgta gcctttgcgg
     2221 cggctttgcg gcagggtaac gatgtcatcg atgtggatgt ggcggccgcc ggcgagggtg
     2281 caggcttcgc ggaagccgca gacggcgacg gcgttgtgtt tgccttcttc aaaaatgccc
     2341 agcaggcggt agccttgggg gcgttggact ttgttgatct gttcggtaaa gcggttgatg
     2401 tcggtcaggg cggaacgcaa aacgctcaag gctgcaaagg cggtggcggt gtcgtccgcg
     2461 ccgatttcgc gcaaaacgta ggatgcgccc gagacggtct gttcctgtgc tttctcggcg
     2521 gcgtgttttt cttcgattgc ctgtgccagc aggacgcgct cgtcggtagg gtcgttttgt
     2581 ccgccctgtt cgcgttcttc gagcagggct ttgcagtcga tgacgcgcag gtcgttgtcg
     2641 gcggcaaagt ccatcaggaa gcggaacatc tgggggttgt cggtttccag ttttttatcg
     2701 acggcgacgc agcggatgtt gtccaccagc atggggcgca cccatttcga gtaggaaagc
     2761 ctgttgtctt tggtaaacga ggacagaatg ccgcacaggc gttccgccca gtcgctggga
     2821 cggaaaatct tgccggaact cgttgtgccg tggatgacga cttcgtaggg gttgcagact
     2881 aacatggcgg cttcctgaaa agaaatgtct ggcgcgatta tacctgatgc ttatgcgggc
     2941 gtgtttggat atgccgtctg aaaagtacgg gattcgtgcg gtaaaacttt gcggcggcaa
     3001 atgtgcgata atacgcgccg tattgccgct tttgcgaagc tgttccgcaa acactcgggc
     3061 ggcgtggacg tataaccgga tgcccgcctg acgcgggttt tttacggaag gggggcaaaa
     3121 atgcctaatc cgctttacag acagcacatc atctccattt cggatttgtc gcgcgaacag
     3181 ttggaatgcc tgcttcagac ggcattgaag ctgaaggcgc atccgcgcgg cgatttgttg
     3241 gaaggcaaac ttatcggttc gtgctttttc gagccgtcca cgcgcacgag gctgtcgttt
     3301 gaaacggcgg tgcagcggct gggcggcaag gtcatcggct tttcggacgg cgcgaatacc
     3361 agtgccaaaa aaggcgagac gcttgccgat accgcccgca tcatttccgg ctacaccgac
     3421 gcaatcatcc aacgccaccc caaagacggc gcggcgcgcg tggcggcgga gttttcgcgc
     3481 gtccccgtca tcaacgccgg cgacggcacg aaccagcatc ccagccagac tttgttggat
     3541 ctggtaacga tttatgaaac gcaggggcgt ttggacaagc tcaaaatcgc catggcgggc
     3601 gacttgaaat acgggcgcac cgtgcattcg ctttgtcagg cgttgaaacg ctggggctgc
     3661 gaatttgcct ttgtttcgcc gcccagcctc gcgatgcccg actacattac cgaagagttg
     3721 gacgaagccg gctgccgata ccgtatcctc ggcagtttgg aagaggcggc ggaatgggcg
     3781 gacatcctgt atatgacccg cgtccagcgc gaacgtttcg atgaacagga atttgccaaa
     3841 atccaaggca aattcaacct cgaagcgtct atgctcgccc acgccaaacc gaacctgcgc
     3901 gtgctgcacc ctttgccgcg cgtggacgaa atccatcccg atgtcgatgc cacgccgcat
     3961 gcctattatt tcgagcaggc aaccaacggc gtttatgcgc gtatggcgat attgtcgctg
     4021 gtgttgaacg aagaagtgtg aggaaccgat atggaaaccc cgaaactcag tgtcgaagcc
     4081 attgaaaaag gtacggttat cgaccatatt cccgctggca gggggctgac catcctgcgc
     4141 cagttcaaac ttttgcacta cggcaacgca gtaaccgtgg gcttcaacct gcccagcaaa
     4201 acccaaggca gcaaagacat catcaaaatc aaaggcgtgt gcttggacga caaagccgcc
     4261 gaccgccttg ccctgtttgc acccgaagca gtggtcaaca ccatcgacaa tttcaaggtt
     4321 gtgcagaaac ggcatttgaa cctgcccgac gaaatcgccg aagtgttccg ctgtccgaac
     4381 acgaattgcg ccggccacgg cgagccggtc aaaagccggt tttatgttaa aaaacacaac
     4441 gggcagacgc ggctgaaatg ccattactgc gaaaaaacct acagccggga ttcggtggcg
     4501 gaagcctgac ggattccctt aaaccgagcg ggcggcattt cgtctgccgc ttgttttgcc
     4561 aatctgaaat ggaatgatga tgcacgcttc tgtccaaagc cgtttcgcac cgatacttta
     4621 tgttttgatt ttctttgccg gttttttgac cgcgcaaatc tggttcaatc agaaagccta
     4681 tactgaagag ctgcctccgc ttctgtccgc attgtccgct gtcgcgctgg tgtggctggc
     4741 gtgggcgttc gtgtcggcgc gttcaaaggc taaggcggaa aagttctacc gcgaaaaaat
     4801 gatacagaac gaaagcatac accccgtcct gcacgcttct ttgcaacact tggaacacaa
     4861 gccgcaaatg ctcgccctgc tggtcaaaaa ccacggcaaa gggatggcgg aacaggtcag
     4921 gttcaaggcg gaagtgctgc ccgacgacga agacgcgcgc acgattgccg ccgagttggc
     4981 aaaaatggat atgtttgcat tggggacgga cgcggtcgcc tcgggcgaaa cctatggacg
     5041 cgtgttcgcc gatattttcg agttgtcggc ggctttggaa gggcgcgcgt tcaaaggaat
     5101 gttgaaactg acggcggaat ataaaaacat cttcggcgat gcctgccgtt cggaaacggc
     5161 gttggagttg ggcgcgctca atcaggcgtt gcaggagatt tcaaaaacat cggaaaagtc
     5221 caaacggata ttttattgaa gatggaaaaa tgccgtctga aacggaaggt gtttcagacg
     5281 gcatttttgt cggatgatta attattcgga gcggttgaag ccaaacttca cgcggctgcg
     5341 gccctgatcc ggtatattgt ccaaatcgcg tcccggattg gcggcggtgt cgcctacgga
     5401 aatatcggag atgttttcca aaatgatggc ggacgacagg tgttcggagg tgcggtaaac
     5461 cattgccaag cccacttctt cggcaggagt ggaaatcagc tcgacggtat ccctgctttt
     5521 gaaattgttg gagaggtcga cctgcatcgt tttcttgcgt ttgtagaggc tcaaaaccgt
     5581 gcctttgtcc aaaccgtccg cctcgccttt gtcgatggtg atggtttgaa actggccggc
     5641 aatccttgtg ccttcaaaca cggaaacgat tttagcctga accgggcggg acggttcgtg
     5701 cggcatcatg ttgaagcggt cggtgtcttc cggcattttc atcaggtagt cgccctgctg
     5761 tatttcggaa atggcggttt cgaccaccag cggctgtatc gaaggggtgc gcagcggggt
     5821 aatcaaagga tgggtgcggg tatggtattc gttgtctttc agccgttctc cagcctgttt
     5881 cgagcgttgt tcgagggcgg agtcggtata gtcgagggag cgcacgatgc cgctgaatgc
     5941 gacttcctgc ccgaggaatt tacccgtatc cggatcggtg atgtttttat tgattcggta
     6001 ggtcaggtag cggcccggct ctttcaggcc tttggtgtaa accctggtgc ctttggtgta
     6061 cagcagcctg ccttccgggc ccgagagcag gcgcggcgcg gcagcggttt ctttgcggga
     6121 aacgatttgc ggatgccgca taaagatgcg gtagaagttg acatcgatgg cgggaatacc
     6181 gtatccggac acttccttat ccggactcat tttgacgacg gggatgccgt ctgtctgttc
     6241 caagccgagg cgcggttcgc cgtcaacgtg gcgcaacacc aatacctggt ccggataaat
     6301 caggtcggga ttgtggattt gatcccggtt cgcgtcccac aggcggctcc attgccacgg
     6361 gctgtacagg tatttgcccg aaatgcccca cagggtgtcg ccctgtttga ccgtgtagcg
     6421 ttccggcgcg ttcgggcgca cctccaaatt tgccgccaaa gtttgtgttg agaatgccat
     6481 acctgccgcg cagagcaggg ttataatacg acgttgcata accgttcccc ttatctgata
     6541 aatttcggtt tgtcttgctt gattgggttg gaaaaagcgg cggcagcccc tcgggatgtg
     6601 ccgcgtgata aaaaatgttc cgcattttaa catcgaatta tccgcaccat cacggtaatt
     6661 atgaaaaaca ggcggcgtat ccgccgaagg aaagagaaaa ttatggcttt attgaatatc
     6721 ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agtcgacgag
     6781 cgcatccgga agctgattgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg
     6841 ctggcggcga cgcaggtcga tgtgcacgag cgcgtggtcg tgatggattt gaccgaagac
     6901 cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact
     6961 tacgaagagg gctgcctgtc cgtgccgggc atttacgaca ccgtaacccg cgccgaacgc
     7021 gtcaaggtcg aggctttgaa cgaaaaaggc gaaaagttca cgctggaggc ggacggcttg
     7081 ctggcgattt gcgtgcagca cgagttggac cacctgatgg gcatcgtgtt tgtcgaacgc
     7141 ctgtcgcagc tcaagcaggg acgcatcaaa accaagctga aaaaacgtca gaaacatacg
     7201 atttgaccct tttgccgtgc cgtctgaacg ctgcaaagtt ttcagacggc acggtcttgt
     7261 ccgacaacct tacgcatacg caggaatatg ctatgaaagt catctttgcc ggcacgcccg
     7321 attttgccgc cgccgcctta aaagccgttg ccgccgccgg ttttgaaatc ccgctggtgc
     7381 tgacccagcc cgaccgtccg aaagggcgcg gtatgcaact gaccgccccg cccgtcaaac
     7441 aagccgcgct ggaactcggt ttgcgcgtgg cacagcccga aaaactgcgt aacaacgccg
     7501 aagccctgca aatgctcaaa gaggtcgagg cagacgtaat ggtggttgcc gcctacggtt
     7561 tgattctgcc gcaggaagtg ttggatacgc cgaaacacgg ctgcctcaac atccacgctt
     7621 cgctgttacc ccgttggcgt ggcgcggcgc cgattcaacg cgcgattgaa gccggcgatg
     7681 ccgagacagg cgtgtgtatt atgcagatgg acatcggttt ggacaccggc gatgtggtca
     7741 gcgaacaccg ctacgccatc caaccgaccg ataccgccaa cgaagtccac gacgcgctga
     7801 tggaaatcgg tgcggcggcg gttgttgccg atttgcaaca gcttcaaagc aaaggccgtc
     7861 tgaacgcggt caaacagccc gaagaaggtg ttacttacgc ccaaaaacta agcaaagaag
     7921 aggcgcgtat cgattggagc gaaagcgcgg cggttatcga acgcaaaatc cgcgccttca
     7981 accccgtgcc tgccgcgtgg gttgagtatc agggcaagcc gatgaaaatc cggcgggcgg
     8041 aagtggtggc gcaacaaggc gcggcaggcg aagtgttgtc ctgttcggcg gacggtttgg
     8101 tcgttgcctg cggcgaaaac gcgctgaaga ttaccgaatt gcagcctgcc ggcggcaggc
     8161 gtatgaatat cgcggcgttt gcggcgggac ggcatatcga agcagggacg aagctgtaaa
     8221 tcccttcaga cggcattccg atccgcaaac gggaatgccg tctgaaacca tcagttgaag
     8281 aaagcgaatc acataatatg agtatggcac ttgcccaaaa acttgccgcc gacagcattg
     8341 cggcggttgc cgaaggacgc aaccttcagg acgtgttggc gcaaatccgc accgcgcatc
     8401 ccgaccttat ggcgcaggaa aacggcgcgt tgcaggacat cgcctacggc tgccagcgtt
     8461 atttgggcag tttgaaacat atgctcgcgc agatgctgaa aaagccgatt ggcaatccgc
     8521 agctcgaaag cctgcttttg gcggcgttgt accagctgca ttacacgcgc aacgcgcccc
     8581 acgccgtggt caatgaggcg gtggaaagca tcgcgaaaat cggacgcggg cagtaccgtt
     8641 cgtttgccaa cgcggttttg cgccgctttt tgcgcgaacg cgacaagctt gtggcttcct
     8701 gtaaaaaaga cgatgtagcg aaacacaacc tgccgctgtg gtgggtggct tacttgaaaa
     8761 accattatcc gaaacactgg cacaacatcg ccgccgcgct gcaatcccat ccgccgatga
     8821 ctttgcgcgt caaccgccga cacggcaatg ccgaaagcta tttggaaaaa ctggtggcgg
     8881 aaggtatcgc ggctaaggcg ttggacgaat atgcggttac gttggaagaa gccgtgccgg
     8941 tgaaccgcct gcctggtttt tcagacggca ttgtttcggt acaggacttc ggcgcgcagc
     9001 aggcggcgta tttgttaaac ccgaaagacg gcgaacggat tttggacgcg tgcgccgcgc
     9061 cgggcggcaa gacggggcat atcttggaac tggcggattg ccgtgttacc gccttggaca
     9121 ttgatgcagg ccgtctgaaa cgggtggaag acaatatcgc gcgtctgggc tttcagacgg
     9181 catcgacggc gtgtgccgat gcacaggacc tgtcggcatg gtatgatggg aaaccgtttg
     9241 atgccgtcct tgccgacgtg ccgtgtaccg cctcgggcgt ggcgcggcgc aatcccgacg
     9301 tgaaatggct acgccgtccg accgacgcgc tcaaaaccgc ccgccagcag gaagccctgc
     9361 tagatgcatt gtggcaggtg ctgaaaagcg ggggaaggat gttgatcgct acctgttccg
     9421 tgttcgtcga ggaaaacgac ggacaattgc aaaaattcct caaccgccat gccgatgcag
     9481 aactgatcga atcgcgggtg cttttaccga acaaacacca agatggcttt tattacgcgc
     9541 ttattcaaaa gcattaaaca atggcttgtg ctgctgccga tgctttccgt tttgccggac
     9601 gcggcggcgg aggggataga tgtgagccgc gccgaagcga ggataaccga cggcgggcag
     9661 ctttccatca gcagccgctt ccaaaccgag ctgcccgacc agctccaaca ggcgttgcgc
     9721 cggggcgtgc cgctcaactt taccttaagc tggcagcttt ccgccccgat aatcgcttct
     9781 tatcggttta aattggggca actgattggc gatgacgaca atattgacta caaactgagt
     9841 ttccatccgc tgaccaaccg ctaccgcgtt accgtcggcg cgttttcgac agactacgac
     9901 accttggatg cggcattgcg cgcgaccggc gcggttgcca actggaaagt cctgaacaaa
     9961 ggcgcgctgt ccggtgcgga agcaggggaa accaaggcgg aaatccgcct gacgctgtcc
    10021 acttcaaaac tgcccaagcc ttttcaaatc aatgcattga cttctcaaaa ctggcatttg
    10081 gattcgggtt ggaaacctct aaacatcatc gggaacaaat aatgcgccgt tttctaccga
    10141 tcgcagccat atgcgccgtc gtcctgttgt acggactgac ggcggcaacc ggcagcacca
    10201 gttcgctggc ggattatttc tggtggattg ttgcgttcag cgcaatgctg ctgctggtgt
    10261 tgtccgccgt tttggcacgt tatgtcatat tgctgttgaa agacaggcgc gacggcgtat
    10321 tcggttcgca gattgccaaa cgcctttccg ggatgtttac gctggttgcc gtactgcccg
    10381 gcgtgtttct gttcggcgtt tccgcacagt tcatcaacgg cacgattaat tcgtggttcg
    10441 gcaacgatac ccacgaggcg cttgaacgca gcctcaattt gagcaagtcc gcattgaatc
    10501 tggcggcaga caacgccctc ggcaacgccg tccccgtgca gatagacctc atcggcgcgg
    10561 cttccctgcc cggggatatg ggcagggtgc tggaacatta cgccggcagc ggttttgccc
    10621 agcttgccct gtacaatgcc gcaagcggca aaatcgaaaa aagcatcaac ccgcacaagc
    10681 tcgatcagcc gtttccaggt aaggcgcgtt gggaaaaaat ccaacgggcg ggttcggtca
    10741 gggatttgga aagcataggc ggcgtattgt acgcgcaggg ctggctgtcg gcgggtacgc
    10801 acaacgggcg cgattacgcc ttgtttttcc gtcagccggt tcccaaaggc gtggcagagg
    10861 atgccgtctt aatcgaaaag gcaagggcga aatatgctga gttgagttac agcaaaaaag
    10921 gtttgcagac ctttttcctg gcaaccctgc tgattgcctc gctgctgtcg atttttcttg
    10981 cactggtcat ggcactgtat ttcgcccgcc gtttcgtcga acccgtccta tcgcttgccg
    11041 agggggcgaa ggcggtggcg caaggcgatt tcagccaaac ccgtcctgtt ttacgcaatg
    11101 acgagttcgg gcgtttgacc aagttgttca accacatgac cgagcagctt tccatcgcca
    11161 aagaagcaga cgagcgcaac cgccggcgcg aggaagccgc cagacattat cttgaatgcg
    11221 tgttggaggg gctgaccacg ggcgtggtgg tgtttgacga acaaggctgt ctgaaaacct
    11281 tcaacaaagc ggcggaacag attttgggga tgccgcttac ccccctgtgg ggcagcagcc
    11341 ggcacggttg gcacggcgtt tcggcgcagc agtccctgct tgccgaagtg tttgccgcca
    11401 tcggcgcagc ggcaggtacg gacaaaccgg tccatgtgaa atatgccgcg ccggacgatg
    11461 ccaaaatcct gctgggcaag gcaaccatcc tgcccgaaga caacggcaac ggcgtggtaa
    11521 tggtgattga cgacatcacc gttttgatac acgcgcaaaa agaagccgcg tggggcgaag
    11581 tggcaaaacg gctggcacac gaaatccgca atccgctcac gcccatccag ctttccgccg
    11641 aacgtctggc gtggaaattg ggcgggaagc tggatgagca ggatgcacaa atcctgacgc
    11701 gttcgaccga caccatcgtc aaacaggtgg cggcattgaa ggaaatggtc gaagcattcc
    11761 gcaattatgc gcgttcccct tcgctcaaat tggaaaatca ggatttgaac gccttaatcg
    11821 gcgatgtgtt ggcattgtat gaagccggtc cgtgccggtt tgcggcggag cttgccggcg
    11881 aaccgctgat ggtggcggcg gatacgaccg ccatgcggca ggtgctgcac aatattttca
    11941 aaaatgccgc cgaagcggcg gaagaagccg atgtacccga agtcagggta aaatcggaag
    12001 tggggcggga cggtcggatt gtcctgacgg tttgcgacaa cggcaaaggg ttcggcaggg
    12061 aaatgctgca caacgccttc gagccgtatg taacggacaa accggcggga acgggattgg
    12121 gtctgcccgt ggtgaaaaaa atcattgaag aacacggcgg ccgcatcagc ctgagcaatc
    12181 aggatgcggg tggggcgtgt gtcagaatca tcttgccaaa aacggtaaaa acttatgcgt
    12241 agcagcgata ttttaattgt agacgacgaa atcggcatcc gcgacctgct gtcggaaatc
    12301 ctgcaggacg aaggttattc ggtcgcattg gcggaaaacg ccgaagaggc gcgcaagctg
    12361 cgccatcagg cgcgtccggc gatggtgctg ctggatattt ggatgcccga ttgcgacggc
    12421 atcacccttt tgaaggagtg ggcgaaaaac gggcagctta atatgccggt cgtcatgatg
    12481 agcgggcatg ccagcattga cacagcagtg gaagccacca aaatcggtgc gctcgacttt
    12541 ttggaaaaac cgatctccct gcaaaaactg ctgtctgccg tcgaaaacgc gttgaagtac
    12601 ggtgcggcgc aaaccgaaac ggggcctgta ttcgacaagc tgggcaacag tgcggcgatt
    12661 caggagatga accgcgaagt cgaagcggcg gcaaagcgca cgtcccctgt atttttgacg
    12721 ggtgaggtgg gttcgccgtt tgaaaccgta gcgcgctatt tccataaaaa cggtacgccg
    12781 tgggtcagtc cggcaagggt cgaatatctg atcgatatgc cgacggaact gttgcagaaa
    12841 gctgaaggcg gtgttttata tgtgggcgat attgcccaat acagccgcaa tatccaaacc
    12901 ggcattgcct ttatcatcgg caaggcagaa cgctgccgcg tcagggtggt cgcatcgggc
    12961 agcagggcgg caggttcaga cggcattgcc tgcgaggaaa agctggcgga actgctgtcg
    13021 gaatcggtcg tccgtattcc gccgctgcgt atgcagcatg aagacattcc cttcctgata
    13081 caggggattg cctgcaatgt ggcggaaagc caaaagattg cgcctgcctc attcagtgaa
    13141 gaggcacttg ccgcattgac ccgttacgac tggccgggaa atttcgacca actgcaaagc
    13201 gtcgttgcaa cgctgttgtt ggaggcggac ggacaggaaa tcggcgcagg ggcggtttct
    13261 tcccttttgg ggcagaatgt gcctgccgag ggagcggaag atatggtggg cgggtttaat
    13321 ttcaacctgc ccctgcgcga attgagggag gaggtggagc ggcgttattt cgagtaccac
    13381 atcgcccaag aaggtcagaa tatgagcaaa gtggcgcaga aagtcggttt ggaacgcacg
    13441 cacctttacc gcaaactcaa acagctcggc atcggcgttt cgcgccgggc gggggaaaaa
    13501 accgaagaat aggcccggac ggccggtttt ccggctgcgg gcttttgttt tcagacggca
    13561 tttggtgcaa atgccgtctg aaatcgtaag gggacggatt ttatgacaga ggacgaacgt
    13621 ttcgcgtggc tgcaattggc gtttacgccc tatatcggcg cggaaagctt cctgctgctg
    13681 ctgcgccgtt tcggcagcgc gtcaaacgcc ctgtccgcac cggcggaaca ggtcgccccg
    13741 gcggtgcggc acaagcaggc ggccgaggct tggcgcaatg cggaaaaacg cgcctcggcg
    13801 cggcaggcgg cagaagcggc gttggaatgg gaaatgcggg acggatgccg tctgatgctg
    13861 cttcaggatg aagatttccc cgaaatgctg acgcaggggc tgaccgcgcc gccggttttg
    13921 tttttgcgcg gcaacgtgcg gctgctgcac aagccttccg ccgccatcgt cggcagccgc
    13981 catgccacgc cgcaggcgat gcggattgcc aaagatttcg gcaagtcgtt gggcgggcaa
    14041 aatatccccg ttgtgtcggg tatggcttcc ggtatcgata ctgccgccca tcagggcgca
    14101 ctggaggcag aaggcggcac catcgccgtg tgggggacgg gtatagaccg catttacccg
    14161 ccgtccaata aaaaccttgc ctatgaaatc gccgaaaaag gattgattgt cagcgagttt
    14221 cccatcggta cgcgcccgta tgccggcaac tttccgcgcc gcaaccgcct gattgccgcg
    14281 ctgtcgcagg ttacgctggt ggttgaagct gcgttggaat ccggttcgct gattaccgcc
    14341 ggcttggcgg cagaaatggg gcgcgaagtg atggcggtac ccggttcgat agacaatccg
    14401 cacagcaaag gctgccacaa actgattaaa gacggcgcga aattggtgga atgcctggac
    14461 gacatcctga acgaatgccc ggggctattg caaaatacgg gtgcttcatc atattctata
    14521 aataaggata cgcccgacac gggcagtcgc gccgttcaga cggcatacgc cccgccgcct
    14581 gccgcaaaaa tgccgtctga aggggcggca ggcggcacag ccgtcggcgg catactggat
    14641 aaaatgggtt tcgaccccat ccatcccgac gtgcttgccg gacagttggc tatgcctgcc
    14701 gcagatttgt atgccgcact gttggaattg gaattggacg gcagcgttgc cgcaatgccc
    14761 ggcggcagat accagcgtat ccgaacttga acgcacttta tattaaggaa cacgaatgac
    14821 cgaagtcatc gcctacctca tcgaacattt ccaagatttc gatacctgtc cgccgcccga
    14881 agacttgggt atgctgcttg aagaagcggg tttcgatacg atggaaatcg gcaacaccct
    14941 gatgatgatg gaagtattgc tcaacagctc cgaattttcc gccgaacctg ccgacagcgg
    15001 cgcattgcgc gtgtacagca aagaagaaac cgacaacctg ccgcaggaag tgatggggct
    15061 gatgcagtat ctgattgaag aaaaagccgt cagctgcgaa cagcgggaaa tcatcatcca
    15121 cgcgctcatg cacattccgg gcgacgaaat taccgtagat accgccaaag tgctgaccct
    15181 gctgctttta tgggcaaaca agagcgagct gcccgtgttg gtcggcgacg agctgatgag
    15241 cgcgctttta ctcgacaaca aacccacgat gaactgaagc ggcttcagac ggcccgcccg
    15301 agtccgtctg aaacgtcggc atcaaaacca ccatccagag aacgacaaat ggcgaaaaac
    15361 ctattaatcg tcgaatcccc gtccaaagcc aaaaccctga aaaaatattt gggcggcgat
    15421 tttgaaatcc ttgcatccta cggacacgtc cgcgacctcg tccccaaaag cggcgcggtc
    15481 gatcccgaca acggctttgc gatgaaatac caactcatca gccgcaacgg caaacacgtc
    15541 gatgccatcg tcgccggagc caaagaagcc gaaaacatct acctcgccac cgacccggat
    15601 agggaaggcg aagcgatttc ttggcatctt ttggaaatcc tcaaatccaa acgcggcctg
    15661 aaaaacatca agccgcagcg tgtcgtgttc cacgaaatca ccaaaaacgc cgtgctcgat
    15721 gccgttgccc atccgcgcga aatcgaaatg gacttggtcg atgcgcaaca agcccgtcgc
    15781 gctttggact atttggtcgg ctttaacctt tcgccattgt tatggaaaaa aatccgccgc
    15841 ggtttgagcg cgggtcgtgt gcaaagtccc gctttgcgcc tgatttgtga gcgtgaaaac
    15901 gaaatccgcg cgtttgaagc gcaggaatat tggacggtgc atctcgacag ccacaaaggc
    15961 cgcagcaagt ttaccgccaa actcgcccaa tacaacggcg cgaaactcga acaattcgac
    16021 ctgccgaacg aagccgcgca agccgacgtg ttgaaagaac tcgaaggcaa agaggccgtc
    16081 gttaccgcca ttgaaaagaa aaagcgcagc cgcaatcccg ccgcgccgtt caccacttct
    16141 accatgcagc aggatgccgt gcgcaaactc ggcttcacca ccgaccgcac catgcgtacc
    16201 gcccaacagc tttatgaagg tatagacgta gggcagggcg cgatcggtct gattacctat
    16261 atgcgtaccg acagcgtgaa tttggcggat gaagccttaa ccgaaatccg ccattacatc
    16321 gaaaacaaaa tcggcaaaga atatctgccg agtgccgcca aacaatacaa aaccaaatcc
    16381 aaaaacgccc aagaagcgca cgaagccatc cgcccgactt ccgtgtaccg cacgcccgaa
    16441 agcgtcaaac ccttcctgag cgcagaccag ttcaaactct atcaaatgat ttggcagcgt
    16501 accgtcgcct gtcagatgac gcccgccaaa ttcgaccaaa ccaccgtcga tattaccgtc
    16561 ggcaaaggcg tattccgcgt aaccggacaa gtgcagactt tcgcaggctt cctcagcgtt
    16621 tacgaagaaa gcagcgacga tgaagaaggc gaagacagca aaaaactgcc cgaaatgagc
    16681 gaaggcgaca aattgcccgt ggacaaactc tacggcgaac aacactttac cactccgccg
    16741 ccacgctaca acgaagccac gctggttaaa gccctcgaag aatacggcat cggccgcccc
    16801 tcgacctacg ccagcatcat ctccacgctc aaagaccgcg aatacgttac ccttgagcaa
    16861 aaacgcttca tgcccaccga cacaggcgac atcgtcaata aattcctgac cgaacacttc
    16921 gcccaatacg tcgattacca cttcactgcc aaactcgaag accagcttga cgaaattgcc
    16981 gacggcaaac gccaatggat tcccttgatg gacaaattct ggaaaccgtt catcaaacaa
    17041 gtggaagaaa aagaaggcat cgaacgcgcc aaatttacca cgcaggaact tgacgaaacc
    17101 tgccccaaat gcggcgaaca caaactgcaa atcaagttcg gcaaaatggg tcgcttcgtt
    17161 gcctgcgccg gttatccaga gtgcagctac acccgcaacg tcaacgaaac tgccgaagaa
    17221 gccgccgagc gcatcgccaa agccgaagca gagcaagccg aacttgacgg gcgcgaatgc
    17281 cccaaatgcg gaggacggct ggtgtacaaa tacagccgaa ccggcagcaa attcatcggc
    17341 tgcgccaact atccgaaatg caaacacgtc gagccgttgg aaaaaccgaa agacaccggc
    17401 gtccaatgcc cgcaatgcaa aaaaggcaac ctcgtcgagc gcaaatcccg ctacggcaaa
    17461 ctgttttaca gttgcagcac ctatcccgac tgcaactacg ccacctggaa cccgcccgtt
    17521 gcagaaacct gtccaaaatg cgcgtggccg gttttgacca tcaaaaccac caaacgctgg
    17581 ggtgtggaaa aagtctgccc gcaaaaagaa tgcggctgga aagaacagat tgaaccgcct
    17641 gcaccgaaag agtgaagcgg ttggggttga gatgaaagaa aatgccgtct gaaaggggtt
    17701 tcagacggca ttttcttcat gtgaaaacaa acctactcag attgaaactg tttacacact
    17761 tctatattaa tctgtataaa gtcctgccac gacatttgtt ctgataagta atggtgataa
    17821 tagtcctcca cacgacagac caaagggcgc gtttcataga tagtgcataa gcgcgttgaa
    17881 tcatcgaaat ggcggcaggt accatctccc cgatccaggt atatggtttg ttcgcttaaa
    17941 ttgacctttt gacagcacaa accgcaagca gagcatggga agggttctga tgaggcaatc
    18001 aaaaatcgat tactttcaca ttgtcgtttt tcaaagctgc aatcagattg tccacctgtt
    18061 tcagtggaga atctttggca atgctcacaa tgccgttaat tgctgtatca atgacgtgga
    18121 tatttcccgc ttccattcct ttgtccaatg cctcaaataa accgtcaatc attttgcgac
    18181 gttctttgaa cgtattttcc agatacaagc gcaaaatttc ttgttggttt ttcaattctg
    18241 ctaatcggac atcacgccat gcaccaatga cttcacgttt ggttttctct tgtgcagcaa
    18301 ttttcttata ttcgttgcgt gcttctaaga tgcgaaaaaa cgaattagaa gctgcatcaa
    18361 acggaatggt tgctggattc tttgttggta gcttattaag gatttggatt tttcccatta
    18421 tactgtacct aattcgatat tcagacaacc ttcgcattga tgacggatat tggctacagg
    18481 agatccgtcc tcttccataa taggcgtatt cagcagattt ttaatcgcct tgccgatgac
    18541 cagcatggta tggaaatcag gatggtcggc atggtcgaca cgaacctttt cgaaacgttt
    18601 aaccatttct tctaaggtat ggcgtatttc ccttgcgttt tccttcaacc cttctaatcc
    18661 ttctgtaatt aaatccatct cgtcaatagc ctcatcgatt tcagcactat atgctcgtgc
    18721 ttccgtcagt gcctcttccg ccttgcttgc cagcataaat ccaccgattg ctaaagcagg
    18781 tcctgccacg atgccaccca aagccaccat gccgcctgcc ataccgaatc cacctgctgc
    18841 caatgagcca cctccgagcc aagcaagggt tgcattggtt gccgcagcac ctgaaagggt
    18901 ggagattgct gttcctgtgg aagcagttgc aatcataggt actgcaccgt atacgccaaa
    18961 gcctgccagt gcgccagtaa tggcactact gccgagattg gtgcttaact ctaaagaagt
    19021 ttgcaccata gtttccattt cttttaattc agcaatatca aatgtactgt taaaacccga
    19081 cagttcgctt ttcgcatgct tgttgctggt gttgtttagg acttgaacaa tatgtctgat
    19141 ttgattgtta aaaatatcaa ccttaaactg tcccagctcc tcaaaagcaa ggttggtttc
    19201 gtctttagtc gaattcaatt ctcttttcct gcgtttataa cggcgttctg cacgctcacc
    19261 tatttctttt gcatcatcta tatcgctgag tgcagctgct cctttgacaa tacctgttgc
    19321 agcaagggcg gcggctgcac cccaaagtaa aactggaata gccatgtgta atttcctaaa
    19381 gtgtataaaa agttaaaaat cttgaagttt caatgtgtat tgtaatttta aaattctatt
    19441 aaaaatacat ttgccattcc agcaaactaa agtttaaaat ctatccaaat aatttaacca
    19501 tctatacaga aagttcagct catggaaacc cacgaaaaaa tccgcctgat gcgcgaattg
    19561 aacaaatggt cgcaggagga tatggcggaa aagctggcga tgtcggcagg cgggtatgcc
    19621 aaaatcgaac ggggcgaaac gcagttgaat atcccgcgtt tggagcagtt ggctcagatt
    19681 ttcaaaatcg atatgtggga cttgctcaaa tcgggcggcg gcgggatggt gtttcagatt
    19741 aatgaaggtg atagtggtgg cgatattgcg ttgtatgcgt caggtgatgt ttcgatgaaa
    19801 atagaatttt taaaaatgga gttgaaacat tgtaaagaaa tgttggaaca aaaagacaaa
    19861 gaaatcgagt tgctccgcaa gctgaccgaa accgtttaaa cagatatgcc gtctgaaaaa
    19921 agttttcaga cggcatattc tttgacaggt cttgtataat accgtttgaa cttacaggtt
    19981 tttgattatg gcggcaggca aacataccaa acacagcaac caggtacgca ttatcggcgg
    20041 gcaatgtcgg ggcaggaaat tgagttttgc atccgccaac ggactgcgtc caacccccga
    20101 tagcgtgcgt gaaaagctgt ttaactggct gggacaggat ttgacgggta aaacggtttt
    20161 ggatctcttc ggaggcagcg gcgcactcgg tatagaagcc gcttcgcgca acgccaaacg
    20221 cgtgctgatt tcggataaca accgccaaac cgtgcagacc ttgcagaaaa acagtcgcga
    20281 actgggtttg gggcaggtgc aaatcgtctg ttcagacggc atcgcatatt tgaagaccgt
    20341 atccgaacag tttgatgtcg tctttctcga cccgccgttt gcatggcagg actggcaaat
    20401 cctgttcgat gccttgaagc cgtgcctgaa cccccgggca ttcgtctatc tcgaggcggg
    20461 taggctgccg gatatccccg attggctgac ggaatataga gaagggaaat cagggcagag
    20521 tacattcgaa ttaagggttt tccaagtggc tgaataatat gcgctttgat aatcatttcc
    20581 gagttgtaaa cattcgtttg caaccgtccg gttcaaaaaa accttgtgct ataatccgcg
    20641 cccgcccggt tttgataatt tagtggaaaa ggaaaagaaa tgtcgctttt tattaccgac
    20701 gagtgcatca actgcgacgt atgcgaaccc gaatgcccca atgatgccat ttcccaaggc
    20761 gaggaaattt acgaaatcaa ccccaacctc tgcacgcagt gcgtcggaca ctacgatgag
    20821 ccgcagtgcc agcaggtttg cccggtggac tgcatcctga ttgacgaaga acatcccgaa
    20881 acccatgacg agttgatggc gaaatacgaa aagattatcc agtttaaata aattcttttt
    20941 aaaacatcaa attatgtctg ttttgaaata aaatcaaaaa aaaacttgac ggaaaagcaa
    21001 gccgctaata aactaacgtt ctcttttgga gggattcccg agcggtcaaa gggggcagac
    21061 tgtaaatctg ttgcgaaagc ttcgaaggtt cgaatccttc tccctccacc aaaattctta
    21121 cttggggcag tagcgagtaa tgcgggtgta gctcaatggt agagcagaag ccttccaagc
    21181 ttacggtgag ggttcgattc ccttcacccg ctccaaacaa ttaggcccat gtagctcagg
    21241 ggtagagcac tcccttggta agggagaggc cggcagttca aatctgccca tgggcaccat
    21301 ctctcgatta ttcatttctt taaggcttag atatatagga tattgccatg gctaaggaaa
    21361 aattcgaacg tagcaaaccg cacgtaaacg ttggcaccat cggtcacgtt gaccatggta
    21421 aaaccaccct gactgccgct ttgactacta ttttggctaa aaaattcggc ggtgctgcaa
    21481 aagcttacga ccaaatcgac aacgcacccg aagaaaaagc acgcggtatt accattaaca
    21541 cctcgcacgt ggaatacgaa accgaaaccc gccactacgc acacgtagac tgcccgggcc
    21601 acgccgacta cgttaaaaac atgattaccg gcgccgcaca aatggacggt gcaatcctgg
    21661 tatgttccgc tgctgacggc cctatgccgc aaacccgcga acacatcctg ctggctcg
//
LOCUS       DAXHMX010000016        21602 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHMX010000016 DAXHMX010000000
VERSION     DAXHMX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.016"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(55..1221)
                     /gene="odhB"
                     /locus_tag="ACFM4S_000464"
     CDS             complement(55..1221)
                     /gene="odhB"
                     /locus_tag="ACFM4S_000464"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6050204.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPVAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAGARPEE
                     RVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGVKLGFM
                     SFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDADQMSIA
                     DIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAT
                     KERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLDL"
     gene            complement(1321..4149)
                     /locus_tag="ACFM4S_000465"
     CDS             complement(1321..4149)
                     /locus_tag="ACFM4S_000465"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6050205.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMTLQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNASKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNGSVKRVVLCAGQVYYDLEAGRTERKLEDDVAIVRVE
                     QLYPFPYEEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4354..5637)
                     /gene="gltA"
                     /locus_tag="ACFM4S_000466"
     CDS             complement(4354..5637)
                     /gene="gltA"
                     /locus_tag="ACFM4S_000466"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6050206.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(5749..5997)
                     /locus_tag="ACFM4S_000467"
     CDS             complement(5749..5997)
                     /locus_tag="ACFM4S_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6050207.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6001..6708)
                     /locus_tag="ACFM4S_000468"
     CDS             complement(6001..6708)
                     /locus_tag="ACFM4S_000468"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6050208.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDDLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6828..8591)
                     /gene="sdhA"
                     /locus_tag="ACFM4S_000469"
     CDS             complement(6828..8591)
                     /gene="sdhA"
                     /locus_tag="ACFM4S_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6050209.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGEDYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8594..8935)
                     /gene="sdhD"
                     /locus_tag="ACFM4S_000470"
     CDS             complement(8594..8935)
                     /gene="sdhD"
                     /locus_tag="ACFM4S_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6050210.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(8929..9306)
                     /gene="sdhC"
                     /locus_tag="ACFM4S_000471"
     CDS             complement(8929..9306)
                     /gene="sdhC"
                     /locus_tag="ACFM4S_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6050211.1"
                     /translation="MSVKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9589..10992)
                     /locus_tag="ACFM4S_000472"
     CDS             complement(9589..10992)
                     /locus_tag="ACFM4S_000472"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6050212.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDDHTQITAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKSVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVYWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTNPQIGFVGLKYAQVTAQYQPDEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11241..11978)
                     /locus_tag="ACFM4S_000473"
     CDS             complement(11241..11978)
                     /locus_tag="ACFM4S_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6050213.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPNWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12254..14530)
                     /gene="metE"
                     /locus_tag="ACFM4S_000474"
     CDS             complement(12254..14530)
                     /gene="metE"
                     /locus_tag="ACFM4S_000474"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6050214.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQVAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     QYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14668..15546)
                     /gene="metF"
                     /locus_tag="ACFM4S_000475"
     CDS             complement(14668..15546)
                     /gene="metF"
                     /locus_tag="ACFM4S_000475"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6050215.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15746..16021
                     /locus_tag="ACFM4S_000476"
     CDS             15746..16021
                     /locus_tag="ACFM4S_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6050216.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16021..16146
                     /gene="ykgO"
                     /locus_tag="ACFM4S_000477"
     CDS             16021..16146
                     /gene="ykgO"
                     /locus_tag="ACFM4S_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6050217.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16252..16452)
                     /locus_tag="ACFM4S_000478"
     CDS             complement(16252..16452)
                     /locus_tag="ACFM4S_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050218.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            16595..17734
                     /locus_tag="ACFM4S_000479"
     CDS             16595..17734
                     /locus_tag="ACFM4S_000479"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6050219.1"
                     /translation="MSQNASVGIVMPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17731..18312
                     /gene="metW"
                     /locus_tag="ACFM4S_000480"
     CDS             17731..18312
                     /gene="metW"
                     /locus_tag="ACFM4S_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6050220.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18429..19274
                     /locus_tag="ACFM4S_000481"
     CDS             18429..19274
                     /locus_tag="ACFM4S_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6050221.1"
                     /translation="MKNKTSSLLLWLAAIMLTACSPNKEDKTKETGASAASSPASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPDSGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLTQGSEETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19323..19883)
                     /gene="efp"
                     /locus_tag="ACFM4S_000482"
     CDS             complement(19323..19883)
                     /gene="efp"
                     /locus_tag="ACFM4S_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6050222.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(19926..21074)
                     /gene="earP"
                     /locus_tag="ACFM4S_000483"
     CDS             complement(19926..21074)
                     /gene="earP"
                     /locus_tag="ACFM4S_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6050223.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTALVPDIVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSLQESVQKYFWFMGFSEKSGGLIRERDYRNA
                     VRFDTEALRERLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21093..21371)
                     /locus_tag="ACFM4S_000484"
     CDS             complement(21093..21371)
                     /locus_tag="ACFM4S_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050224.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         5332 a         5497 c         5455 g         5318 t
ORIGIN      
        1 ctataaaaat tcagacggcc ttttcattaa caaataaaag gccgtctgaa acgattacag
       61 atccaacaac aggcgggctg ggtcttccaa cgcgtcttta atggctacca aagtcaatac
      121 agcttcgcga ccgtcgatga tacggtggtc gtaagacaga gccagataca tcattggacg
      181 gacaacaact tgaccgtttt caaccacagc gcgctcttta gtggcatgca tacccaaaat
      241 cgcagattga ggcgggttga tgatcggggt agacatcata gaaccgaaag taccgccgtt
      301 ggtaatactg aatgtaccgc cggtcagatc ttcgatagcg attttgccat ctttggcttt
      361 tttcgcgtaa tcaacaattg cttgttcgat gtcggcaatg ctcatttggt cggcatcgcg
      421 caggattggc acaaccaaac cgcgtgggct gccaatcgcg ataccgatgt cgaagtagcc
      481 gtggtacacg atgtctttgc cgtcaacaga agcattcaca accgggtatt ttttcagggc
      541 ggcgacagcg gctttaacga agaaggacat aaagcccaat ttcacgccgt gttctttctc
      601 gaatttttct ttgtacttcg cacgcaagtc catgattggt ttcatgttga cttcgttgaa
      661 tgtagtcaga atggcgtttt cttgttgaga agccaagagg cgttctgcaa cacgggcacg
      721 caggcggctc attggtacgc gttcttcagg acgtgcgccg gcaggggctg cggctgcggc
      781 aggtttggca gcggcatttt gtacgtcttc tttcaatacg cgaccgtcac ggccggaacc
      841 ttgcaatgcg ttcacgtcaa caccggtctc cgcagccagt ttggcggcgg caggcatagc
      901 ggcgttgttt tgtgcggcag caggagcggc agccggggcg gcttcggcag gagcggctac
      961 gggtgcttcg gcagcggcag tagcagctgt atcgatgcgt gccaaaactt ggtcggcaac
     1021 aacggtttca ccgtcttgcg ctacgatttc aaccaatacg ccggcttgtg gggaagggac
     1081 ttccaaaacc actttgtccg tttcgatatc gatcaggatt tcgtcacggg caactgcttc
     1141 gccaactttt ttcttccatt ccaagagcgt gccttcagat acgctttcag acagcatagg
     1201 tacttttaca tcaataatca ttttgtgtct ccaatggccc tttcagacgg cctgttgtgt
     1261 gtttttcatt ctgcatatgg gcattccgcc tgagcagcct cagacggaat gccacttggc
     1321 ttacaacgcc aaagcgtctt caaccaattg tttcaattgg gcaatatgtt tgcttgagta
     1381 gcccactgca ggcgatgcgc tgcttggacg accggcataa gacagttttt gctcttcgct
     1441 gataacgtct tcgatgcggt ggcggatttg gtagaacgca ccttggtttt tcggctcttc
     1501 ttgtgcccaa accacagatt ttgcgttcgg atatttcgcc agttcggctt taacctcctc
     1561 gtatgggaac ggatacagct gctcaacgcg gacaatcgca acatcatctt ccagtttacg
     1621 ctcggtacgg cctgcttcca agtcatagta aacctgaccg gcacacaata ccacacgttt
     1681 cacgctgccg ttgcttgcgc gttcggcggt atcaccgata accggacgga aagtcgaacc
     1741 ttcggtgaag ttttccagcg ggctcattgc acctttgaag cgcaacaggc gtttggacat
     1801 gaaaatcacc agcggtttgc ggtatgaacc caaaacttgg cgttgcaaga ggtggaacat
     1861 ttgcgacgct tcagacggca tgatgacttg catattgttc tcggaacaca gttgcaacca
     1921 acgttctacg cgtgcagagg agtgctcggg gccttgaccg tcgtagccgt gcggcaggat
     1981 ggtggtcaga ccgcacaaac gaccccattt ggtttcgcct gaagacagga attggtcaat
     2041 agtcacttgc gcgccgttgg cgaagtcacc gaattgagcc tcccaaatgg tcagcttgtc
     2101 aggcgcggag caggcaaagc cgtactcgaa cgccatcacg gcttcttcgt tcaaaatgga
     2161 gtcgataacc aggaactcgc ccataccttc gcccatatgg cgcagaggga cataagtacc
     2221 gtcgtcccat ttttcgcgtt tttgatcgtg caataccgca tgacggtgtg agaatgtacc
     2281 gcgcccagag tcctcgccgg aaatacgcac gccatggcct ttggttacga ggctggcgta
     2341 tgcgagggtt tccgccatac cccagtcgat ggcttgtttg ccggatgcca tggctttacg
     2401 cgcttcaatc acacgttttg cagtcggatg cagggcaaag ccttccggta cggcggtaaa
     2461 cttctcagtg agacgctcaa tatctgcggc aggcaaaccg gtttcgatgt gttcgcgcca
     2521 atctttgcct tggtatttgc tccagtcgat ttgcgtgcgt tgaaagttgc tcaacgttgt
     2581 ttgctcgacg tgttcgcctt tgtccaaagc atcacggtag gcttggatgt aaccgtctgc
     2641 ctcggcttgg gttaccacgc cttcggcaat cagttgctcg gtgtacaaag cacgcgcacc
     2701 ggggtgttgc gatacttttt tgtacatcat cggttgggtc aaggtcggat catcgccctc
     2761 gttgtgaccc catttacggt agcagacaac gtcaatcacg atgtctttat ggaatttttt
     2821 gcggtaatcc aaagcggctt ggatggcaaa gcaaacacgc tcaggatcat cgccgttcac
     2881 atggataacc ggagcggaaa ccatttttgc gatatcggta cagtgtacgg ttgaacgggt
     2941 atcgcggata tcggaagtgg taaagccgat ttggttgttg atgacgatat gaaccgtacc
     3001 gccggtggta taaccgcgcg ttttagacag gttgaatgtc gcttggttga ctcccagacc
     3061 gataaatgcg gagtcgccgt gaatcaatac cggcaagact ttgtcgcggc cgttttcgcc
     3121 caaacgtttt tgtttggcgc gcgcagaacc ttccaccacc gggttgacga tttctaagtg
     3181 agacgggttg aacgccaaag aaacatgcat cgggccgtgc ggggtagcaa tatcggagct
     3241 gaagcccata tggtatttca cgtcgccgct gggcagtttg atttcggcac gaccttcaaa
     3301 ttcggcaaac aaatcgccgg gttttttgcc caaaatgttc accaaaacat tcagacggcc
     3361 acggtgcgcc ataccgatga tgacttcttc cacgccgtct ttactggcgt tttgaatcag
     3421 gtagttcaaa ccggcaatcg cgctttcgcc gccttcgaca ccgaaacgtt tctgaccgac
     3481 atatttggta tgcagataac gttccaaagt ttcggcagca gtcatctctt tcaagatacg
     3541 gcgtttttga tcggcattgt aatgcggtgt ggacaatacg ctttcaaaat aattgcgtac
     3601 ccagcggcgc tcttcggtat tgggaatata gatatattcc aatgcgatgt ggccgcagta
     3661 ggtttgtttg aggttgctga tgatttggga caaaggcagt ttgccgcgat tggcaaaatc
     3721 gccctcgccc atattgaatt gaagcgtcat atcggcatct gacagaccgt ggaatttcgg
     3781 atcgagggct tcaatatcgc gcggggggat acgtttgagc ggatcaagtt gggctgcacc
     3841 cacgccttgg atacgatagg cagaaatcag ccgtaaaacg ctgacttgct ttttcagcat
     3901 tgcctcatcc gcaccgcccg caacggcaga tgcaattttc tttttcgcca aagtaacaaa
     3961 tgattcgcga atcggtgtgt gtgcgacatc gacagcaacc gtccccggct gtttgctcaa
     4021 atcggtgaaa tactgcttcc atttttcatc aaccgcatcg gggttttcca aaaaagcctc
     4081 gtacaattcc tcaatgtagg gtgcgttcga accgaacagg taagagaaat tgagtttttc
     4141 gtccatcatg gcgtgcgctc ttttctttaa aatataaaca aagggtagaa gtccgcgtcc
     4201 tgacggctga actgatgccg tctgaaaaga cattttattc taccctattt taaaccggat
     4261 tgcctgtatt gatttcatcc gactacaatc aaatgtatac agaaagcaat ccgattctct
     4321 aatattgcct gtttgacaat atattaatgt atgttacctc tctcctgccg gcacataatc
     4381 gcggcgttcc gaaccagtat aaagctggcg cgggcggccg attttcagcg aaggatcgct
     4441 gatcatctcg tgccagtgcg aaatccaacc tacgctgcgc gacagggcga agatgacggt
     4501 aaacatttcg gtcgggatgc ccagcgcgga caggacgatg ccggaataga aatcgacgtt
     4561 tggatacagc ttgcgttcga taaagaacgg gtctttcagc gcaatctgtt ccaattccat
     4621 cgccagtttg aatttcggac tgtcttccaa gcccaattcc ttcaaaactt catagcaggt
     4681 ttcgcgcata atgctggcac gcggatccat attgcggtac acgcggtgac cgaagcccat
     4741 cagacggtat ttgcgttgtt tcacaccttc catgtatgcg gcaacattag acacatcgcc
     4801 gatttcgtcc aacattttca acacggcttc gttcgcaccg ccgtgcgaag caccccacag
     4861 gcaggcgata ccggcagcaa tacaggcaaa cggattcgca cccgaagagc ctgccagacg
     4921 gacggttgaa gttgaggcgt tttgctcgtg gtcggcatgc aaaataaaga tgcggtcgag
     4981 cgcgcgtgcc aaaacgggat tgggtttgta gtcttcacac ggcgtggcga acatcatatg
     5041 aaggaagttt tcggaataag aaagattatt cttcggataa ttgaacggca gaccgtttga
     5101 atagcggtag cacattgccg caatggtcgg gattttggaa atcaggcggt aaatcgcgat
     5161 tttgcggtgt tcgggattgc taatgtccaa gctgtcttgg tagaacgcag acagcgcgcc
     5221 gaccacgccg accatcatcg ccatcggatg cgcgtcgcgg cggaaccccc ggaagaacca
     5281 agtcagctgt tcatgcacca tcgtgtggcg gcggacggta ttgtcaaatt ctgccttttg
     5341 ctcgggagtc ggcagttcgc cgtaaatcaa caggtagcag acttccaaat aatcggattt
     5401 ttcggccagc tgctcgatgg ggtatccgcg ataataaagc aagccttgat cgccgtcgat
     5461 gtaagtaatt ttagactcac agcttgcggt tgaaacaaat ccggggtcga aggaaaacaa
     5521 acctgtattt tttgtcagcc cccgaatgtc aaccacatcg tgcccgatgc tggcttccaa
     5581 taccggcagc tccaaacctg cctgacccgg tacgttgagt ttgattgatt tggacatatt
     5641 attgctcctt tgcaagttat aacccacgtt actgcatccg cagcacgttc tttgccttca
     5701 gacggctttt gcattttgaa atctgcctgc aggcagattt cagatgcctc atgcccgtct
     5761 gattttttca agcatcggga taaggtgccc tttgtccgtt tccgaatgcc cgttaatcaa
     5821 ggcaagcaat tcttgatctt gaaattcaag gatttcggaa aactcggaca gctctttatc
     5881 gctcaaatgc tcgaattctt tttccataaa cctgccgaag attaaatcta actccaacaa
     5941 tccccggcgg gtttgaaaac ggattttccg tttggcaata tcgtcaaaaa ccatcatttc
     6001 ttaaacggcc cgcttcaaca taatctcttt aatcttaccg atggctcggg tcggattcaa
     6061 gtgtttgggg catacgtcca cacagttcat aatggtgtgg cagcggaaca aacggtatgg
     6121 gtcgttcaga tcatccaaac gctcattagt gatggtatca cggctgtccg caatgaaacg
     6181 gtaagcattc agcaaaccgg acggaccgac gaatttatca gggttccacc aaaatgacgg
     6241 gcaggcagtc gaacagcagg cgcacaaaat acactcgtac aaaccgtcca actctttacg
     6301 ctcttcctga gtttgcagac gctctttgtc cgcatcaatc ggattatcgt tgacaacata
     6361 aggtttgacg gaatggtatt gtttgaagaa ctgggtcata tccacaatca ggtcgcggat
     6421 aacaggcaga cctggcagag gacgaatttt aaccggctgt ttcaagccac gcagatcggt
     6481 cagacacgcc aaaccgtttt tgccgttgat gttcataccg tccgatccgc aaatgccttc
     6541 gcggcaggaa cggcggaaag acaatgtatc atcttgtgct ttcaggcgca ctaaagcgtc
     6601 caaaagttta acgtcggttg gttccaattc caactcgtaa cgctgcatat aaggtttggc
     6661 atcaacgtcc gggttgtaac ggtaaatttc aaaactcatt ttttccatga gaagtgttcc
     6721 ttttctcata aactgcccaa tgattttgta ttatgaattc aagcagttca atgtgggtat
     6781 ggttagattt cagacgacct ttgaggcgaa gaccgtctga aaacgcatca ataaacgcgc
     6841 ttggccggtt tgatgtattc cacgctcaag ggcttggtat gcaccggttt gtaagacaag
     6901 gtattggtat ctgaatggta cagcgtatgt ttcatccagt tttcatcatc gcgctcagga
     6961 tggtcgtctg aagcgtgcgc accgcgtgat tctttacgtg cttcggcaga caccaaagtc
     7021 gctttcgcca cttcaatcag gttatccaat tccaaagcct cgatacgcgc ggtattccac
     7081 actttgctct tgtctttgat ttcggtacgt ttcacacgct cggcaatcgc catgacttct
     7141 cgaacgcctt tgctcagaat ctcatcagta cggaacacgc cggcgtgcag ttgtacagag
     7201 cgttgcagtt cgcgacgcaa tgcatcaacg ttttcaccat cggtttggtt gtccaaacgc
     7261 tcgatacgtt ggcgggtcaa ctcacctgca ttagcaggca aaggtttcca gtcgctttgc
     7321 tctttgatga atttaatcat gctgtcgccg gcagctttac cgaataccac caagtccaac
     7381 agggagttgg tacccaagcg gttcgcaccg tgtacggaag cacaagcgca ctcacctgcc
     7441 gcatacagac cttttacagg cacttcgtaa tcttcacctt gcggaacgac aacttcgccg
     7501 tggtaattgg tcggaatgcc gcccatcata tagtgggtag tcggcacaac gggaatcggg
     7561 tctttaatcg gatcgatacc ggcgaactga atggaaatct cgcggatgcc cggcagtttt
     7621 tccataattt tttctgcgcc gatatggtcg attttcagta agacatggtc tttgttttta
     7681 ccgcagccgc gaccttcgta gatttccatc gccatcgcgc gggaaacaac gtcgcgagaa
     7741 gccaagtctt ttacggtcgg cgcatagcgt tccataaagc gttcgccgtc ggcattcaac
     7801 agaataccgc cctcgccgcg tacgccttcg gtaatcaaca cgcccgcacc cgccacgccg
     7861 gtcgggtgga attgccagaa ttccatgtct tccaacggga tacctgcacg cgcacaaata
     7921 cccaaaccat cgccggtatt catataggca ttggtagaag acgcataaat acgaccgccg
     7981 ccgccggtag caaacatcac agctttagcg tggaaaatat aaacttcgcc ggtttccatt
     8041 tccatggcgg ttacgccgac gacatcgccg ttttcatcac gaatcaaatc ttgtgccgtc
     8101 cattccacaa agaattgcgt attggcacgg acgttttgtt ggtacaaagt atgcagcatc
     8161 gcatgacctg tacggtcggc aaccgcacag gcgcgttcta ccgcgcgttt accgtgttcg
     8221 gcagtatggc cgccgaaagg acgctgataa attttaccgc tttccacacg gtcaaaaggc
     8281 atacccatgt gttccaactc aattacggct tcaggcgcgg cgcggcacat aaactcaatc
     8341 gcatcttggt cgcccaacca gtcggaacct ttcacggtat cgtacatgtg ccagtcccaa
     8401 cggtcttcct gcacattacc cagagaggcg gaaataccgc cctgcgccgc tacggtatgc
     8461 gaacgggtcg ggaacacttt agacaaaacg gcacaattca gaccggattt ggataattgg
     8521 agggctgcgc gtaaacctgc accaccaccg ccgacaatca cggcatcaaa cttgcgaaca
     8581 ggaaaaccca tacttacccc caaatcactt taactgaata cacgagacag ccgaccagcc
     8641 aaacgatggt ggcaacctgc aaaaacaaac gcacgccgaa gggtttgata tagtccatcc
     8701 acaaatcgcg gatacccacc caagcgtgca agaatacggc gatgaagctc acttgggtaa
     8761 atacttttac ccaagtttga ctaaaaaatg cctgccatgc cgaatattct ttaggcaggg
     8821 aaaatagaac cactaaaagt gcaacggtat aaatcaacat aataaccgca gtcgcacgtt
     8881 gcatcaccca atcgcgcaaa ccgtaatggg caccggtcaa tttacgttct accataacaa
     8941 cgctcccaaa acgacagtca aaaccaatgc agaagcaaat acggctttag cggtattgcg
     9001 cgcagtattc agctcaaggc ctttgtgcgc atccaaaaat aaaaagcgga taccggcaag
     9061 ggaatggtgc agataagccc acaatacgcc gattaaaacc agcttgacca aaggatggga
     9121 aacaatggca cggtaagttt caaatgcaga ctcttgactc agggtgccgg acaggaaata
     9181 cagcaggaaa ggcagcataa taaacagccc gaccccgctg atgcggtgaa ggatggaaac
     9241 tatcccgggt atcggcagac ggatgttcgg caaatccaga taaacaggac gcggtttgac
     9301 agacatagtc aattcctctg tagttatata tcgactcaaa aaccgtaaac ccattgctac
     9361 actgggttac ataaacgtta atttacctgt tttatcccat tttgaacagt gtattttaaa
     9421 aacaaactga tagattttga caaacacgcg tgaaaacagc agattaaaag aaagattttt
     9481 agttttgccc ccttcccgac acggtatcag catccgacct ccaaccccat cctcaaaaat
     9541 aaaatgccgt ctgaaaaaat ttcagacggc attgttttgc catatcggtc aggctttcaa
     9601 tttcgcatcg gcatcacgca acgcagtacg cagaccttcc tcgataacag gatggtagaa
     9661 cggcatatcc agcatttgcg gaacggtcat cttcatttga tgcgcccatg ccaacaggtg
     9721 cgccaaatgt tcggcggcag ggcctacgat ttccgcacca atgaagcggc cggttgcttt
     9781 ttcggcatac aggcgcatat ggcctttgtt taccagcatc acgcggctgc ggccttggtt
     9841 tttaaacgat acttcgccga tgacaaattc gtcgggttgg tattgcgcgg taacctgcgc
     9901 gtatttcaaa ccgacaaagc caatttgcgg attggtaaac accacgccga tggtgctgcg
     9961 gcgcaaaccg ccgccgatat tcggatagcg gcccgcgttg tcgccggcaa tcttgccttg
    10021 atcggcagct tcgtgcagca ggggcagttg attggatgcg tcgcccgcaa taaagatatg
    10081 cggaatactg gtctgcatgg tcagcggatc ggcaacgggt acgccgcgcg catctttgtc
    10141 gatattgatg ttttccaaac cgatattgtc aacgttcgga cggcggccta cggctgccag
    10201 catatattcg gcaacaaata cgcctttttc gccgtcttgc tcccaataga cttctacatt
    10261 gccgtccgca tcgagtttga cctcggtttt ggcatccaga tgcagtttca attcttcacc
    10321 gaacacggct ttcgcctcgt ctgaaacgac agggtcggaa atgccgccga tgatgccgcc
    10381 caagccgaaa atctcgactt tcacgcccaa acggtgcaat gcctgcccca gttccaaacc
    10441 gataacgccc ggtccgaaca cggcaacgct cttaggcagc gtatcccatg agaaaacgtc
    10501 gtcattgata atcaaacggt cgcccaaaga ctgccactgc ggcaggataa cgggacgcga
    10561 gccggtagca atcacgaaac tttttgccgt gatttgggta tggtcgtcga tttggacggt
    10621 atgctcgtcg ataaatttag ccgaacccat gatgcgtttg tcagcaggcc attcttccac
    10681 atcggcaacg acaaagccga caaaacgatc gcgctcggat ttaacgcgct gcatcacttc
    10741 ttcgccattg acgacgatac tgtctttatc caaatgcacg ccgaacgggt cggtatgcaa
    10801 tgcgtgatgg cgcgcctcgg cggcggcaat caagagtttg gaaggcatac agcccacgcg
    10861 cgcgcaggtc gtgccgaaca cattgttttc aatcaggtaa acattgtccg aatgtaaacg
    10921 ggcattgcga aacgcgccca taccggcagt accgccgccg attacgacga catctgcttg
    10981 aatttttttc ataatctttg tccttccgtt ttcgtctgaa acactgccga cacggaatat
    11041 atcggcttca gacggcattt aaatattttc ccaatcaaaa acattattca gataaataaa
    11101 aacaaaattt cgattgagtg caacaatatc caaatcctct tataaatata gagttattcg
    11161 gagcggtagt gtagaaccgc ccgaataact ctatccggta tatcagacag tttaatcaaa
    11221 ccgccttaag caaacagcta tcagttttta gccaagtaag cttccaaatc ttcgctgccg
    11281 ccgatgtatt taccgccgat gaagacttgc ggggcagtca tcttgccggt aatggcgcga
    11341 acggaagtaa cggttgcatc tttgcccaat acgatttctt cgtaagacaa acctttgtct
    11401 tgcagggctt gtttggcttt agcacagaat tggcagccgg gtttggtaaa gatggcaaca
    11461 gactcttgag ccttccaatt gggagcaacg aatttcagca tagtatctgc atcggatact
    11521 ttgaacggat ctcctggttc ttccggctcg atgaacattt tttcaaccac gccgtcgtta
    11581 accagcatgg agtaacgcca agaacgtttg ccgaagccca agtcttcttt accgaccaac
    11641 atgcccatgc cttcggtgaa ttcgccgttg ccgtcaggaa tcatgtagat gttgtcggat
    11701 tcttcttcgg ccgcccaagc gttcattacg aaagtatcgt ttacagatac gcagtagatt
    11761 gcgtcaacgc cgttttcttt gaacgcgccg aacaattcgt tgtaacgcgg caggtgtgaa
    11821 gaagaacaag tcggggtaaa tgcaccaggc agggagaata cgactacttt tttgcctttg
    11881 aacaaatcat cggtagacac atctttccaa gtgtcgccaa cgcgggtgcg gaatactacg
    11941 gaaggtactt tttgaccggt acgatcttgc aaagccattt tgagctcctt aatagatagg
    12001 tttaagaaat gattgtgaat cgctgggcat tctaccccga ataagtcgat ttgtataatt
    12061 tatagttcaa ataatattaa ttattcaaaa ctattaatca ccaacctcga ccaactttta
    12121 cagtatataa agccatgcct aaaaaaaaca agtcttattt gagaaaaatt cttagaaact
    12181 cccctacgct catttgaaaa cagaaatgcc gtctgaaaag tattcagacg gcatccgatc
    12241 acgttatccc tgcttattta gccagttcgg cacgcagttt gtgggttacg ttcatcatca
    12301 cttggagttg ttccagagtt tctttccaac cgcgtgtttt caggccgcag tctgggttaa
    12361 cccacagacg ttcaaccggt acaacctcga tggctttgcg caacaggtgc tccacttcgg
    12421 cttctgtcgg tacgcgcggg ctgtggatgt cgtaaacccc cgggccgatg tcgttcgggt
    12481 attggaattc gccgaacgcg gtcaagagtt ccatgtcgga acgtgaagtc tcgatggtga
    12541 tcacgtccgc atccattgca gcaatcgcag gcaggatatc gttgaactcg gagtaacaca
    12601 tatgagtgtg gatttgggtg ctgtcttcgc aaccggcaga ggacaggcgg aaggattcgc
    12661 ccgcccagtt caggtaggca tcccaatcgg cgcgtttcag cggcaagcct tcgcggatgg
    12721 caggttcgtc aatttggatg actttgatgc cggctttttc cagatccaat acttcgtcgt
    12781 tcagagccag tgcgatttgt ttgcacacgg tagagcgagg aatgtcgttg cggacgaaag
    12841 accattgcag aatggttaca gggccggtca acataccttt catcgggcgt ttggtcaggc
    12901 tttgtgcgta agtagaccaa gccacggtca tggcttcagg acggcttacg tcgccaaaga
    12961 taatcggtgg tttcacgcag cgtgagccgt agctttgtac ccagccgtat tgagtgaatg
    13021 caaaaccgct caacaattcg ccgaagtatt caaccatgtc gttacgctcg gcttcgccgt
    13081 gtaccagtac gtccaagtcc agtttttctt gctcttcaac caccaaggcg atttcttttt
    13141 tcatcgcggc ttcgtaatcg gcggcagaca gttcgccttt tttgaaggct gagcgtgcct
    13201 ggcggatttc ggtggtttgc gggaaagaac cgatgttggt agtcggtagc agaggtaggt
    13261 tcaaccatgc ttgttgcgct ttgatacggt cggcaaatgg agatttgcgt tggtctgcgt
    13321 tggcaggcaa atcggccagg cgtttggcaa cgtctgcacg atggatttcg ctgctgttgg
    13381 cacgggagtc ggcagcagct tggctggcgg cgagttcttc ggcaacagaa tcacggcctt
    13441 cgttcaatgc agctttcaga acgcgcaatt cttgggtttt ttgcagggtg aatgccaacc
    13501 aagagtacag gtcgggtttg ttggctttca gtttttcttc aactgacaag tcaaatggag
    13561 tgtgcagcag cgagcaagag ctggaaatcc acaaacggtc acccagtttg gcttgcagag
    13621 gctcgacagt ttccaaaact ttgttcaggt tggcgcgcca aatgttgcgg ccgtcaatca
    13681 cgccggcaga caggactttg tcgtagtcgg cgaacgcgtc cagttgctcg ggggcgcgta
    13741 ccaagtcgat gtgcagaccg tcaacaggca gggctttcaa caatgcggcg tgttcggcaa
    13801 cagaaccgaa gtaagtgctc aacaggattt tggcacttac tttgctcaaa gtagcgtaaa
    13861 cgtctttgta ggcttccacc cattctttag gcaaatcgac agccaaagca ggctcgtcga
    13921 tttgaatcca ctcggcaccg gcttcaacca aagcagtcag gatttcaacg taaacaggca
    13981 acagtttagg caacaggctc agacggtcga attcgacggc gcctttttct ttacccaccc
    14041 acaggaaagt caacggacct acaacggtcg gtttggcttt cagaccgagg gcttgggctt
    14101 cttgcagttg ttgaacatag tgtttggcat tggctttgaa ttcggtatcg gcgtggaatt
    14161 caggcaccaa gtagtggtag ttggtgtcga accatttggt catttcgata gcgaattggt
    14221 ctttgttacc gcgcgccagt tggaagaatt gttccaaaga caggttttgg ctgtcgaagc
    14281 cgaagcgggc gggaatcgcg ccggtggcga cttgcaggtc gaggatgtgg tcgtagaaag
    14341 tgaaatcgcc tacggcaacg aaatcggcgt tggcagcgac ctggtgtttc cagtttttct
    14401 cgcgcaagtc tttagcaaca gccagcaatt cttgctcgct gatttctttg cgccagtatt
    14461 tttcttgtgc gaatttcaat tcgcggaagg cgccgacacg cgggaagcct gagaaatgta
    14521 atgttgtcat gttgaactct ccagttggat tttgtaagga ccggttcaat ccgaatcggc
    14581 cggtattgta aatcagaatg gctcggatgg ggcggtgttt tgctttttag acaccttaga
    14641 tgccgtctga aactgtttga tacggcttta agggcgcacg cctaaaatat ggcaaatggc
    14701 gtaagtcagc tcgctgcggt taagcgtata gaagtggaaa tctttcacgc cttcgcggga
    14761 caggactttg accatatcga tggcgatact tgccgccacc agattgcgcg taccttggtc
    14821 gtcgtccaaa ccttcataca tttgcgacag ccagcttggg attttgacgt tggttacttg
    14881 agccatttta ccgagctgct tgaagttggt aacaggcaaa ataccgggga cgatttccac
    14941 atcgataccc aacatcacgc agcggtcgcg gaagcgcagg tagcgttcca catcgaagaa
    15001 gaattgggtg atgacgtggt tcgcgcccgc atcgattttg cgtttcaaat taatcaggtc
    15061 ggcttgtgcg gatttcgctt cgggatgcac ttcgggatat gctgctacag agatgtcgaa
    15121 gtcggcgacg gagcgtaata gcttaaccaa gtcttcggcg taaaacggtt ttttctcata
    15181 accggccggc tcgtctccgc gcagggcgac aatgcggcgg atgccgctgt cccaataatc
    15241 tttggcaatt tggcgcaatt cgtcgggaga agcgtcgata ccggtcaggt gaggcgcggc
    15301 ttccaagccg gtttcctgtt tgatgcgttt gacgatgccg tgtgtgcggt cgcgctcgcc
    15361 tgagtttgca ccgtaagtta cggaaacaaa tttcgggtgc aaggtttgca ggcgatggat
    15421 ggaatcccac agcatggttt ccatttgttc gtttttcggc gggaaaaatt cgaatgaaac
    15481 gttgatgtcg cctttcaaat cggaaaggct gttatttaac gcattgattt cttttgcgta
    15541 attcatggct gcgtaccttt tattatcttt tatatgatgt ttcaggcaac ataataaagt
    15601 tgcgccaaac atgagtcaat ttcaaaattt tcagatttat catgaaaatt tctaatatat
    15661 tacaaaaata gttaaatttc cttgtacagc ccttctaaac tatgctagag tttgttatgt
    15721 tataacactg aaagcaaggc aatatatgaa accgaatatt catcccgaca attaccgaac
    15781 cgtccttttt ttcgatagca gcgccaatga aggctggtta atccgctcct gtgccgaaac
    15841 acacggaaaa acgatggttt ggacagacgg caaagaatat ccgctgtttt cgctggatac
    15901 ttcttccgca tcccatcccg tctatactgg caaacagcgc aacgtcaaca ccgaaggccg
    15961 tgccagcaaa ttcaaccaac gtttccaatc cgtaatgtca tcgtttagaa aggacaaata
    16021 atgcaggttc tgtcttcact caaaaccgcc aaacaacgcc accgcgactg ccaaatcgtc
    16081 aggcgcaggg gcaaagttta cgtcatctgc aaaagcaatc cgcgttttaa agcgcgccaa
    16141 cgctgacccc ctgcccttcc tgcttcggtt gtaaatcccc tcctaccgca agcccttttc
    16201 aaaaaatgcc gtctgaaaca gaaaaatgcg tttcagacgg cattttttcc gtcaatacga
    16261 atccggaatg tgtttgaaat acagcataaa cagataccaa aacagcgtcg cggcggccgc
    16321 accgagcagc acccgggcac tgatttcggc aacttcgcgc agggcgcgtt tctgcctgcg
    16381 gctgaagagg cggttcataa tcagcgcggc aagtgcgaaa aagaaaaaaa tacggaaaag
    16441 tctgcctatc atgtcgggaa gcgaaaagta tggttcgggc gcggcggcat ggggaaggat
    16501 gtttcagcgc gtcaggctgc ttgtccgacc gtgtcaaaaa gtcatgccgt ttgcgatata
    16561 ataacgcttt ttgccgtatt tgttggaaca ggatatgagt caaaatgcct cggtgggcat
    16621 tgtaatgccc caaaaaattc cgtttgaggt gccgctggtt ttggaaaacg gtaaaacttt
    16681 gccgcgtttc gatctgatga ttgaaaccta cggcgagctg aatgctgaaa aaaacaatgc
    16741 ggttttaatc tgccacgcgc tgtcgggcaa ccatcatgtt gcgggcaggc attcggcgga
    16801 agataaatat acgggctggt gggacaatat ggtcggacct ggcaaaccga ttgatacgga
    16861 acgttttttc gtggtcggtt tgaataatct gggcggctgc gacggcagca gcgggccttt
    16921 gtcgattaat cctgaaacgg gcagggaata cggcgcggat tttccgctgg ttacggtgaa
    16981 ggactgggta aaatcccaag ccgcgcttgc cgattatctc ggcatcgggc aatgggcggc
    17041 ggttgtcggc ggcagcttgg gcggcatgca ggctttgcag tgggcgattt cctatcccga
    17101 acgcgtgcgc cacgccttgg tgattgcgtc cgcgccgaaa ctgtccacgc aaaatatcgc
    17161 gttcaacgat gtggcgcgtc aggcgatttt gaccgatccc gatttcaacg aaggacatta
    17221 ccgcagccgc aacaccgttc ccgctcgggg cttgcggatt gcccgcatga tggggcacat
    17281 cacctatctt gccgaagacg gtttgggcaa aaaattcgga cgcgatttgc gttccaacgg
    17341 ctatcaatac ggctatggcg ttgaatttga agtagaatcc tatctgcgct atcaaggcga
    17401 caaattcgtc gggcggtttg atgccaacac ctatttgctg atgaccaagg ctttggacta
    17461 tttcgatccg gcggcggatt tcggcaacag cctgacccgc gccgtgcagg atgttcaggc
    17521 aaaattcttt gtcgccagct tcagcaccga ttggcgtttc gcgcccgaac gttcgcacga
    17581 actggtcaag gccctgattg ccgcccaaaa atccgtgcag tatatcgaag tcaaatccgc
    17641 acacgggcac gatgcctttt taatggaaga cgaagcctat atgcgtgcgg tcgccgccta
    17701 tatgaacaac gtttataagg aatgtcagca atgaacctgc gcgatgattt gcaactgatt
    17761 tacgaccgga tacccgaagg cagccgcgtc ttggacttgg gctgcggcga cggcgaactg
    17821 cttgccgcat tggtcgaaca caaaaaatgc agcggctacg gcatcgaaat cgacaccgac
    17881 agcgtcatcg ccgcgatgtc gcgcggcgta aatgttatcc aagccgattt ggaagaaggt
    17941 ttaaccgcat tcaacgatca aagttttgat gtgattgtgt tgagccaaac catccaagcg
    18001 atgcagaata ccgaaaaaat cctgcgctgc ctgatgcgcg ttgccaaaca ggcgattgtc
    18061 agcttcccga atttcggcta ctggcgcaac cgcgtccaaa tcgcactcgg cggacatatg
    18121 ccggtttccg aacgtatgcc ctaccattgg tacgacacgc ccaatatcca ttggtgcacg
    18181 ctcaaagact ttgatttgtt gtgcgccaaa aacaacatcc gcgtcctcga acgcgccgtg
    18241 atgacgggca acagacaggt caaacatttc cccaaccttt tgggcagcct ggcgttttac
    18301 cgcgtcgggt aagacaggtt gagaaccttg tcggcaataa caggttcttc ggttctatta
    18361 acaacaggcc gtctgaaaaa tgaaacggca ggttttcaga cggccttttt aatattggaa
    18421 acaatagaat gaaaaacaaa acctcatcac ttctcttatg gcttgccgca atcatgctga
    18481 ccgcgtgttc cccgaacaaa gaagataaaa ccaaagaaac cggcgcatct gccgcttcgt
    18541 ccccggcatc cgccgcttcg tcttccgcgc cccaaaccga tttgcaaccg gccgcatccg
    18601 cccctgataa cgtcaagcag gcagaaagcg cgccgccgtc aaattgcacc gacctgcacc
    18661 ccgccaccgg cattgacgat ctcatgcagc aaatcgccga acacatcgac tcggactgtc
    18721 tgtttgccct ttcccatcac gaactggaaa cccgtttcgg cttacccgac agtggctatg
    18781 acaacataca gcggctgctg tttcccgaca tccgccctga agatcccgac taccatcaga
    18841 aaatcatact ggcaattgaa gacttgcgtt acggaaagcg cacgatcagc cggcaggcac
    18901 aagatgcctt gatggaacag gaacgccgcc tccgagaagc gacgctgttg ctgacacagg
    18961 gcagcgaaga aacccgcggg caaggcgagg agccgaaacg cacgcgttat tttgaagttt
    19021 cggcaacccc tgcctattcg agccggcaca acaacggact tggcggcaat ttccaataca
    19081 tcggccaatt gcccggctat ctgaaaatac acggagaaat gcttgaaaac caatcactct
    19141 tccggctgtc caaccgtgaa cgcaatcccg acaaaccgtt tttagacatc cattttgacg
    19201 aaaatggcaa aatcacgcgt attgtcgttt acgaaaaaaa catctacttc aatccaaact
    19261 tggggcgaag ataaaaaaat gccgtctgaa agcctttcag acggcatttt tcaagtagga
    19321 tatcaggcgc gtttgcggaa ttcgccggtg cgggtatcga tttcgacttt gtcgccgttt
    19381 tcgatgtaag acatcacttg gatttcggtg ccgccgacca gacgcgcggt tttcatcact
    19441 ttgccggaag tgtcgccttt gacagcaggc tcggtgtact cgacttcgcg tacgatgatg
    19501 gtaggcagtt cgacagaaat ggggttgcct tcgtagaagg taacttcgca ttggtcttcc
    19561 ataccgtcaa cgatgaattt caacgcgtcg ccgatgttgt cggcttcgat ttcgtattgg
    19621 ttgaattctt cgtccataaa gacgtacatc gggtcggcaa aatagctgta cgtacagttt
    19681 ttgcgggaca ggatgaccac gtcgaatttg tcgtcggctt tgtaaatggt ttcggaagcc
    19741 gcgccggtca gcaggttttt cagtttcatg ctgactttgg cggaagagcg gccgcctttg
    19801 atgtattcgg ttttttgaac gaccatagga tcgttgccga ccataaatac attgccggcg
    19861 cgcagttctt gtgctgtttt cattgtgatt ttccaatcaa atcggttgtc gtaaaacgcg
    19921 ctattctagc gtattttttg atgctttgaa acaaaggcgg cgagtttttc ggatgcggaa
    19981 ggctgcccga aaagataacg gctccaatcc tccgcgcctt gccgccagcc gttttgatgt
    20041 tgttgcaggg tttgccaaca ttcgaggcgt tgcgtggcgg ataaagcctc tccgccgttg
    20101 aggtcgtctg aaaggcggcg gtgtaccgat gcggtttcgg gcgagtaaaa tccatgcgcc
    20161 ttatcccaaa aggcgtggag tttatcgaga tggacattct cgtcttgcgg gtagatgtgc
    20221 caaaagaagg gtttgccggc aagctgggcg cgcacgaaac tgtcttcgcc gcgtatcacg
    20281 gcgcagtcgg cgaggtgcag cagtttgtcg aagtcctgtt gcggcacgaa agggattttg
    20341 acgaggcgga cggatgccgt ctgaaaaaca tcgccgtcgt tttgcagggc atcttgcgga
    20401 ataacgccgc tttgtttgag gctgtcgatg atttgcgtcc ccgccagcaa cagtgtcatc
    20461 gggctgcctg cctgtcgcca catttccagc cactttgccc aaacatcgct ccgatagccg
    20521 aaaagcagcc attcgggggc gtttttttcg ggaagcatca gccgctctcg caaggcttcg
    20581 gtatcgaaac ggacggcatt tcggtaatcg cgttcgcgta tcaacccgcc gcttttttcg
    20641 ctgaaaccca taaaccaaaa atatttttga acactctcct gcagcgaagg catcagatgc
    20701 agcctttcat tgctttcttc cgcgctcaaa tattcccaat tcagccaaag cggcttgtgt
    20761 cggcggatga tgtgcagcac attttcgggc aggtcgcagg caaaagtttc gataacgata
    20821 tcgggaacaa gggcagtatc gatatccgcc gcatcggaat gccaagtgcg gacatgaata
    20881 tcctgatgaa cgcagggaac atcgggcaaa tcagggcaaa gcgcacgcaa ggcggacaca
    20941 tcgtccgtcc acaaatgcac ctgccaaccg agttcgcggt gcaaaacacg ggcgagccgc
    21001 cacgaaacgc cgatgtcgcc gaaattgtcg atgaccttgc aaaaaatcca acagacaaaa
    21061 ggaggagtat tcatagaaat aacggaaaca agctaaaacg cgccgaaata gtggaacaaa
    21121 tacacaatca gcaaaataat cggaataaac agcaacatag tttgacgttt caaatcaaac
    21181 tcttgtttca aatgatgctt gatggtgtcg aaaaggacga atttaaacaa aaacgtcgct
    21241 tcgcaggcaa ggatgatgcc gttgatcaga tgctggagtt tgtcttcggg cggattggcg
    21301 gcgaaaacgg caagcgtcgc caacgggatg aggcagacgg caagccaacg gacaagcagg
    21361 tttcgagaca ttgcaaggct ttcaaaaaaa gcaaaacgcc attatcgccc caagcgcacg
    21421 ccttgtctaa cggacgcacc ggagatgccg tctgaagcct tgccgcagga aaacaccgca
    21481 ccgccgaacc tttaaatacg cccgagacgg gcgttccccg aaaaatcgga cgctgccgcc
    21541 gatgcctgag gacaaggcgg cagcaggcac gggcatattg aaaacccggc gcgtcggttt
    21601 ga
//
LOCUS       DAXHMX010000017        21110 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHMX010000017 DAXHMX010000000
VERSION     DAXHMX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21110
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.017"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(36..512)
                     /gene="greA"
                     /locus_tag="ACFM4S_000485"
     CDS             complement(36..512)
                     /gene="greA"
                     /locus_tag="ACFM4S_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6050225.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            907..2208
                     /gene="aroA"
                     /locus_tag="ACFM4S_000486"
     CDS             907..2208
                     /gene="aroA"
                     /locus_tag="ACFM4S_000486"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6050226.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNTCEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     ENYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVCVIGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2251..2784)
                     /locus_tag="ACFM4S_000487"
     CDS             complement(2251..2784)
                     /locus_tag="ACFM4S_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6050227.1"
                     /translation="MFPPDKPLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(2839..4365)
                     /locus_tag="ACFM4S_000488"
     CDS             complement(2839..4365)
                     /locus_tag="ACFM4S_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6050228.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(4477..5883)
                     /locus_tag="ACFM4S_000489"
     CDS             complement(4477..5883)
                     /locus_tag="ACFM4S_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6050229.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADM
                     NESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLAGLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            6167..6946
                     /locus_tag="ACFM4S_000490"
     CDS             6167..6946
                     /locus_tag="ACFM4S_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6050230.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            6943..7644
                     /locus_tag="ACFM4S_000491"
     CDS             6943..7644
                     /locus_tag="ACFM4S_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6050231.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            7641..9095
                     /locus_tag="ACFM4S_000492"
     CDS             7641..9095
                     /locus_tag="ACFM4S_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6050232.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(9278..9763)
                     /gene="purE"
                     /locus_tag="ACFM4S_000493"
     CDS             complement(9278..9763)
                     /gene="purE"
                     /locus_tag="ACFM4S_000493"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6050233.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARERGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(9811..10527)
                     /locus_tag="ACFM4S_000494"
     CDS             complement(9811..10527)
                     /locus_tag="ACFM4S_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6050234.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPGSPAAKRAASAIRKR"
     gene            complement(10527..11195)
                     /locus_tag="ACFM4S_000495"
     CDS             complement(10527..11195)
                     /locus_tag="ACFM4S_000495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6050235.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTVLALALPKHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(11206..11391)
                     /locus_tag="ACFM4S_000496"
     CDS             complement(11206..11391)
                     /locus_tag="ACFM4S_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6050236.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            11534..13510
                     /gene="mutL"
                     /locus_tag="ACFM4S_000497"
     CDS             11534..13510
                     /gene="mutL"
                     /locus_tag="ACFM4S_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6050237.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIGLALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCTTMLERLALAHPHIAFSLKRDGKQVFKLPTQSLPERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASDYPTGNKPDTRNAFGASGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELSRLEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFATSHEECAALSDHADALAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLSELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            13605..15725
                     /gene="dnaX"
                     /locus_tag="ACFM4S_000498"
     CDS             13605..15725
                     /gene="dnaX"
                     /locus_tag="ACFM4S_000498"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6050238.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAATSCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPETETAQTSVQTASAAAMPSESKTAEPVSNQENNDVPPWEDAPDEAQTAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSETPSENPIQATPNNEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDNAEIPPPDWEHAAPADAEEENNADEGSNDENNTPS
                     APPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADK
                     KRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADPA
                     AQKILQAFGAQWQPESLELAANRP"
     misc_feature    14794..14833
                     /gene="dnaX"
                     /locus_tag="ACFM4S_000498"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            15805..16140
                     /locus_tag="ACFM4S_000499"
     CDS             15805..16140
                     /locus_tag="ACFM4S_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6050239.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(16972..18018)
                     /gene="recA"
                     /locus_tag="ACFM4S_000500"
     CDS             complement(16972..18018)
                     /gene="recA"
                     /locus_tag="ACFM4S_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6050240.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            18272..19036
                     /gene="aroD"
                     /locus_tag="ACFM4S_000501"
     CDS             18272..19036
                     /gene="aroD"
                     /locus_tag="ACFM4S_000501"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6050241.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPIVTMSMGQTGTVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            19056..21071
                     /gene="rep"
                     /locus_tag="ACFM4S_000502"
     CDS             19056..21071
                     /gene="rep"
                     /locus_tag="ACFM4S_000502"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6050242.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAEMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVVKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIIKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGVRIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQTEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         5104 a         5869 c         5711 g         4426 t
ORIGIN      
        1 cctgcgtgtg tcgggtgtat cgaaatcaag ccgaatcaaa tatatcggac ttcgataatg
       61 tcgtattcgc gcacgccgcc cggggcttgg acttccgccg tatcgccctc ttccttgccg
      121 attaaggcgc gggcaatcgg tgagccgaca tagattttgc cctgtttgat gtcggcttcg
      181 tcttcgccga caatttgata ggtaacgtgt tcttccgttt ccaaatcttc cagcgtaacc
      241 gtcgtaccga acacgatttt gccttcggcg tggatttcgg tcggattgat gatgtgggca
      301 acggaaagtt tgtgttccag ctcggaaatg cggccctcga taaagccttg gcgttctttg
      361 gcggcttcgt attcggcgtt ttcggacaaa tcgccgtgcg aacgggcttc ggcaatcgct
      421 tcgatcactt cgggacgcgc cacgcttttg agctgctgca attcctgttt cagcaattcc
      481 gcaccgcgta cggtcagggg gattttttgc atcggtcttt tttctccata ttccggcaca
      541 ccgttttgcg gcagcaagca taccgcgtac cgtcttgttt tgtgcgtccg gatattaaaa
      601 taaaaataca agccgcccgg aaaatcggcg gcttgtctgt cgttgaacag cggctattct
      661 accaaattct atgaaattgg caatcgtacc gtaccgtccg caaacgccac ctgcccgcaa
      721 caaaagccga aaatatgccg gcaaaaaaat tttagaaaca aaaaatttaa aaataatcaa
      781 ttttcggcat aaaaaaccac attcacggac tttaaaaccg aaaatgccaa acctgagatt
      841 tttcatacag catttgcacc agtataatgc aggctgtttt tatctttata atattgacgt
      901 tttgccatga ccgaatccct ccgcctccct gccgcctcgc tcaaaccttc caccgtcgcc
      961 ctgcccggct ccaaaagcat cagcaaccgc accctgctgc tcgccgcctt gtccgacaat
     1021 acttgtgaaa tccattccct gctcaaatcc gacgataccg accgtatgct cgaagcactc
     1081 gataaactcg gcgttgaaat cgaatatctt gccgaagacc gtctgaaagt gcacggcaca
     1141 ggcggacgct tccccaaccg cactgccgat ttgtttttgg gcaacgcggg cacggcgttc
     1201 cgtccgctga ctgccgcact tgccgttttg ggcggcgatt atcatctgca cggcgtgcct
     1261 cgtatgcacg aacgccccat cggcgatttg gccgatgcgc tgcggattgc cggcgccgat
     1321 gtcgaatatc tcggcaagga acactatccg ccgcttcata tcggcaaacg tcaagacaac
     1381 ggcgaacgcg tgattccgat taaaggcaat gtgtccagcc agtttctgac cgccctttta
     1441 atggcgttgc cgctgaccgg gcaggcgttt gaaatccgta tggtcggcga attgatttcc
     1501 aagccctata tcgacattac tttaaaactg atggcgcaat tcggcgtaca ggttgccaat
     1561 gaaaactacc gcgtcttcaa aattcccgcc gatgcgcact accacgcgcc cgaacacttg
     1621 cacgtcgaag gcgatgcctc cagcgcgtcc tacttcctcg cagccggttt gattgccgcc
     1681 acgcccgtct gtgttatcgg tatcggcgca aacagcatac agggcgatgt cgcctttgcc
     1741 cgcgagctgg aaaaaatcgg ggcggacgtg gtttggggcg aaaacttcgt cgaagtttca
     1801 cgcccgaagg aacgtgccgt ccaatccttt gatttggatg cgaaccatat ccccgatgcc
     1861 gccatgaccc tcgccatcgt cgcgcttgct acagggcaaa cctgcacgct gcgcaacatc
     1921 ggttcgtggc gcgtcaaaga aaccgaccgc atcgccgcga tggcaaacga gttgcgcaaa
     1981 ctcggggcaa aagtcgtcga agaagccgaa gcaattcaca tcaccccgcc cgaaacgctg
     2041 acacccgacg ccgtcatcga cacgtacgac gaccaccgca tggcgatgtg tttctcgctg
     2101 gtttcgctgt tgggcgtacc cgtcgtcatc aacgatccga aatgcaccca taaaaccttc
     2161 ccgacttatt tcgacgtgtt ctcttcactg accgaaacaa cggaataagg cggcattttg
     2221 ccgcgattcc ggcgcggcgc ggcgggcggc tcattctgta aagaaagtat gtgcgccgag
     2281 gtagtttttg gcgtaaaacg gtgtggagag tttttcggtt ttgatggttt tgccgctgct
     2341 gggggcatgg atgaattcgc cgttgccgat atagagtccg acgtgtgagt agcggtgtgt
     2401 gccgccggtg ttgaagaata cgaggtcgcc ggccttaagg cggctgtcgg ggattttgcg
     2461 gcttgccgcc gccatgtcgc gggcggtgcg cggcagcttg acgttgaggg cgtttttgta
     2521 aacgaattga atcatgccgc tgcaatcgaa gccggttgcg gtgctgctgc cgccccattt
     2581 gtagggcgtg ccgatgagtc cgaggctgtg gagcatgagt tcctgcgagc cttgtgtgcg
     2641 gtcgatgtgg ctgatgcgga cggcttggat ttgccggact gtctgtttgg gtttcggttg
     2701 gcggtgtttg ccggaggtcg tgccgcatga ggcgaggagt agtgcgctga gacagaggaa
     2761 aaggggtttg tcggggggaa acatggtttt tcctttgcgg gttcggatat ccgtctgaag
     2821 gtgtttcaga cggcatttct ataataaacc ttctatgggc agcagggata ggatttttgc
     2881 ggcgatgcgt ttccaaagtt tggcttcggg ttcgttcggg taggtttttc gggtggcggg
     2941 atcgtgccat tgcaggcggt tgtgcctgtc gagggtaacg cggtaggcgt attcgggggt
     3001 ggtatcggca agggtgcgct ccatctgttc tgcgattttg gggctttcga taacaacgcc
     3061 catttcggtg ttgagacgcg cggaacgggg gtcgaggttg aacgaaccga tgaagatgcg
     3121 tttgccgtcc acaatgaagg ttttggcgtg caggctggtt acggagctgc cggtcaggcc
     3181 tttgtctttt gtggcaggga cggcatggtt gggttgcagc tcgtagagtt tgatgccggc
     3241 tttgagcagc ggttttcggt atttgacata gccggaatgg acggcggcaa cgtcggtcgc
     3301 ctgtagcgag ttggtcagaa cggtaacgtc tatgccgtcc tgcaccagtt ttgctagtgc
     3361 gtctgtgccg gattttgtgg ggacgaaata gggtgaaacc agatagacgc ttttttcggg
     3421 ctgtttgagc gcgtcttgca gccgcccggc aatcggcggt ttgcggcggt cgcggtcgag
     3481 tccttttgca gggtcgtcgc tgatcaggcg ggtttggacg ctctgccagt cgatgcgtcc
     3541 tgtctgtatt ttttggtaga ggggcgactg ttcgacggtt tcgcggtagc gcaggagcgc
     3601 gtgtctggac gtttcgtcgt tgtatccgag tgcttgaaga cccttgccga tgttgccgct
     3661 gcggatgatg cgcgtggcgt tgtgggcgga atggcttgcc cagtagcgat cgaagtcgtg
     3721 cgatacttcg ccgacgacgc tgccggtagc gaggatatcc aggtcggcga aaacggtgtc
     3781 ctcaccgact ttgaagtatt cgtcgccgat attgcgtccg ccgagtatgg tggcgcggtt
     3841 gtcggcggta aaggatttgt tgtgcatgcg gcggttgagg cgggggaagt cagtcaggta
     3901 gccgagtgcg cgccatttgc ggaagacgaa ggggttgaac aggcgcactt cgatattggg
     3961 atggctgtcg agggcgagca ggaggtcgtc caatccgcgc gtgttgttgt cgtccaacag
     4021 caggcgtacg cgcacaccgc gttctgcggc aaggtacacg aggttgaaca gcagtcggcc
     4081 ggaaatgtcg ttgcgccaga tgtagtattg caaatcgagg ctgtgttcgg cagattcgat
     4141 aagggcggcg cgggcggcaa aggcttcgtg ggggtcgttc agcagataga tatcggatag
     4201 cccgttggta tgaggggtgt gccggatttg caggatgttg tccaggcgga cgggtttgga
     4261 agtattgaaa tgacggcttt ccgtccgttc ttccagtggg ggcaaccatg aagaacatga
     4321 acagagaagg aggcataaaa gggaaattag gctgcgtgtt ttcatcaggg atatggtttc
     4381 agacggcatt gcctgtgttt tggggttggc gcgcatggaa gtgcggaatc ataatccaaa
     4441 cgttgaaacg ggtaaaagtt ttgcgtgcgg accgctttag gacggtgtgt tccgtgtcag
     4501 gtttgcgccg tctgaaacgt gcagacgctt gaaaaccaac gacgaggcaa gggtgatgcc
     4561 gccgatgctg agcagggtca tacggaaggc ggaatgcaga cctgaagaag ccggtatcag
     4621 aaatgtccag tttttaagga ttaatgcgcc gacaacaatg cccatgctga tggcaagctg
     4681 ttggttgacc gccatcaggc tgttgccgct gccggtctgt tgcgggcgca aaccggcgag
     4741 ggtcagtgta ttcatggcag aaaactgcag ggagttgcac gcgccgatcg ccagcgagag
     4801 gaaaatccaa atccacagcg acgagtttcc gtcaggcagg gcgagcagca tgatgaaggc
     4861 ggcaagcagc ttggtgttcc aaagcagtac cgtgcggtag ccgaaacgtt tcatgagcgg
     4921 tgcaatcagc ggtttgacca gcagcgaaga cagggcgacg ggggcgacca gccaacccga
     4981 caggcttgcg ccgaagccga aagcgacttg aaacatcagg ggcatcagaa aaggaatcga
     5041 gctgatgccg agacggctga acagattgcc cgccagtccc agacggaaag tgcgtatcag
     5101 aaacaggtcg gcggaataaa tcggtttgga cgcggttttc atatgtcgga aataacggcg
     5161 tgcaaacagc agtccgccgc acagcggcaa cagtgcaaaa tacggaggca gcgcgtgcga
     5221 caggctttct gccgaaagta acaagaggca cgcggcggca gaaaaaatca gataaccttt
     5281 gaagtctaaa gagatattgc tgcctttaat atcgggcatg atgttgcgtc ccaatatgaa
     5341 acccaaaaga ccgatgggca ggttgagcag gaaaatccag tgccacgaag cgtattcgac
     5401 cagataaccg cccgccaaag gccctaaaac cggtccgatt aatgcgggca taaccgcata
     5461 attgatggcg ttgagcagct tggacttgtc gtacacacgc aagatggtca gacgcggtat
     5521 cggaaccagc atcgaaccgc cgatgccctg aacgacacgg gaaagcgtca attcaaacag
     5581 cgaacccgat gcggcgcaca atgccgatcc gagcataaaa acggcaatcg aaccgaaaaa
     5641 gacttttttc gttccgaacc tgtccgccaa ataaccgctc aaaggaatca gcagggcaac
     5701 cgtcagcgta taggcaacga ctgccagttg catatccaga ggcgactcat tcatgtcggc
     5761 ggcaatttca ggcagtgcgg tatttaaaat ggtcgcatcc aacatctgca taaaaatggc
     5821 aattgccagc agaagcggca gccaagggga tggtgcgcgg gcggataggg tgtttttttc
     5881 catagggcga ttgtacccca tccttgtgcc gttattgttt tcagatgctg tctgaatgcc
     5941 gtcagagtcg gcgtcctgaa tgttcacaag caaacgaatc agcattttat tgtaatgata
     6001 attattatcg aaaattatca gagtaaggta cagtaagcgt tatggggtag tttgtaagaa
     6061 aaaccggatt attttttaaa attagacttg acccgcaaca gtcaattact taaagtaaac
     6121 gcttaccttt ctacagagaa aaacgggttt cccgttatca aaaaacatga gcgcaaccat
     6181 tcccccaaaa atcatccgat acgacagcaa tccgaccgat gtctattttt tcggcacttg
     6241 cgtccttgat ctttttatgc ccgaagcagg catggatgcc attgccctaa tcgagcagca
     6301 gggcatacgc gtccatttcc cgatggcgca aagctgctgc ggccagcctg cctattcatc
     6361 cggccatccg accgaagcct ttgatgtcgc cagagcgcaa ctcgaccttt tccccgagga
     6421 gtggccgatt gtcgtaccgt ccggttcgtg cggcggtatg atgaaacacc attggccgac
     6481 gctgtttaaa ggcagcgagt acgaggaaag ggctgtggat tgcgccggcc gcatcatcga
     6541 gtttacccat ttcctgcttg ccatcggttt caaacccgaa gacaagggcg aaccgctcaa
     6601 agtcgccgtt cacacttcct gcgccgcccg ccgcgaaatg aatgtccatc tttcaggctg
     6661 gcaactgatt gacggtatgg aaaacgtcga acgcatcgtc cacgaccacg aaagcgaatg
     6721 ttgcggcttc ggcggcacat tctccgtcaa acaagccgat atttccggcg cgatggtaac
     6781 cgacaaagtc gccgcgctga aagaaaccgg cgcaaccgaa atcatcagcg cggactgcgg
     6841 ctgtatgatg aacatcggcg gcaaaatcgc caaggacgag ccggatatgc cgcgtccgaa
     6901 acatatcgca tccttcttgt tggaacgcac cggaggcaaa gcatgagcgc gcgtgaaaat
     6961 attttggcaa aactgaaaaa agccgatgcc ttgccgatgg aagagcctgc ggttttcgat
     7021 tattaccgcg aaaagggcgt ttcttgggac agcgaagccg agcgtctgaa acattgggct
     7081 gccgcaatgc gcgcggtcaa aaccgaaatt tattgggtga cgaaaagcaa ttggatgcag
     7141 gttttccgcg aagcggcaga aggcaagggt ttgaaaaaca tcctgctgcc cttggcgacc
     7201 gaacacggac aaattgcccg tgccgcattg gcgggcagca atatcgaacc gattgtcttc
     7261 gagcgcgaaa tcgatacttg gaaaaccgag tttttcacga acatcaatgc gggcttcagc
     7321 ggcgcgcaat gcggcatcgc ccgcaccggc acgctgatgc tgttttccag ccccgaagaa
     7381 ccgcgtactt tgagtctcgt tccgcccgtg catttctgcc tgttcgatac gtccaagatg
     7441 tacaacgagt ttcacaatgc cgtcgaaggc gaaaaactgg tggaaaacgg tatgccgacc
     7501 aatgtattcc tgatttccgg cccgtccaaa accgcagaca tccaactgac gcttgcttac
     7561 ggcgcgcacg gcccgcgcga tttggtcatc ctcgccatcc tgcccgacca catttcccct
     7621 gccgatttgg aggaaaacgc atgactacgc aaaccatcaa atttcacatg aagccggaaa
     7681 ctttcaagca aaacgccgca atttcccttc aagacaagcc tttgcgtaaa agcctgcgta
     7741 ccgcgatgga tatgctgatg accaaacgca aagccgtttt gaccgacgaa gaagagctgc
     7801 aaagcttgcg cgatttgtgc gaacacgtcc gtcagcgctc attgtctaaa ttgcctgccc
     7861 tgctggagca gctggaagaa aacctgacta agttgggcgt aaaagtacac tgggcggaaa
     7921 caccggccga agcctgcgaa atcatccacg acatcatcac agccaaaaac ggcaagctga
     7981 tggtcaaagg caaatcgatg gtcagcgagg aaatcgagct gaaccattat cttgaagcaa
     8041 aaggcattaa agcggtagaa agcgacttgg gcgagttcat cgtccaaatg gcaggcgaaa
     8101 aaccgaccca tattgtgatg cccgcaatcc ataaaaccaa agaacaggtt agcgaactgt
     8161 tccaccaaaa cctcggtacg ccgctgacag acgatgtaga ccaactgacc ggcttcgccc
     8221 gtaaagcact gcgcgatatt tacagcactg ccgatgtcgg tttgagtggc gtaaactttg
     8281 ccgttgctga aacaggtacg ctgtgtctgg tggaaaacga gggcaacggt cgcttgagta
     8341 ccaccgtacc gcccgtgcat atcgctatta ccggcattga aaaagtggtg gcgaaattgt
     8401 ccgacatccc acccttgtac agtctgctgc cgcgctccgc catcggtcaa aacatcacaa
     8461 cctatttcaa tatgattacc ggcccgcgcc gcagtgaaga attagacggt ccgcaagaaa
     8521 tgcacttggt tctgctcgac aacggccgca gccaggctta tgccgaagac caaatgcgcc
     8581 gcaccctgca atgtatccgt tgcggcgcgt gtatgaacca ttgcccggtt tatacccgca
     8641 tcggcggcgc ggcatacggc acaacctatc ccggtccgat tggcgagatt atttccccgc
     8701 acctgttagg cttggatgcc actcgcgacc tgccgaccgc ctgcacgatg tgcggcgcgt
     8761 gcgtggaagt ttgtccggta cgcatcccga ttaccgaaca aatgcagcgt ttgcgcgttg
     8821 aagcgcaacg ttcgccgacc gaaaccgtgc cgcaccccat ccgggggcaa ggcgcatcgc
     8881 ataccttcgg cgaacaaatg gcgtggcgca cattcaacgg tattttcagc ggcagcaaaa
     8941 cctaccgcgc cttcggttgg gcagccacca agttccgcaa cctgaccccg cgcaaacagt
     9001 tgggttggac gcaaaaccgc gtgccgatga aaccggcgaa gaaaaccctg cacgaactga
     9061 tggcagaaaa aatgcgccaa aaagaacagg cataaaaagt tgttcgcaaa aatgccgtct
     9121 gaaacccgaa acagggcttc agacggcatt tgtatagtgg attaacaaaa atcaggacaa
     9181 ggcgacgaag ccgcagacag tacaaatagt acggcaaggc gaggtaacgc cgtactggtt
     9241 taaatttaag ccactatata ttcgcggacg gcgggtttta aatctgttcc aattccatac
     9301 tcaaaacagc ctgttcctgt ttggctcgga agtctgccag tttttgcgcc agttcggggg
     9361 tttcgttggc gagcatggaa acggcgaaca atgcggcatt tgccgcgcct gcctcgccga
     9421 tggcgaatgt ggcgacgggt acgcctttgg gcatttgtac aatcgataaa agcgaatctt
     9481 cgccgcgcag gtatttgctg gggacgggta cgcccaaaac ggggacggtg gtcttggcgg
     9541 caaccatgcc gggcaggtgc gccgcgccgc ccgcgcccgc gataatggct ttgatgccgc
     9601 gctcccgtgc ggtttcagcg tattggaaca ttaaatccgg ggtgcggtgt gcggaaacga
     9661 cgcgcgcctc atattctacg ccgaactctt caagaaactg cgctgcctgc cgcataacgg
     9721 gccaatcgct gttgctgccc atgatgatgc cgatttgaat cataaatcct ccttggtgcg
     9781 gatggggtaa aaagcggaaa aatggaaaaa ctatcgtttg cgtatggctg aggcggcgcg
     9841 ttttgccgcc gggctgcccg ggtaagcctg tatcagactg cgccaagtcg cccttgcaat
     9901 gtctttctgc tgcaacctgt attggcattc gccgattttg aatatggctt cgggcgcggt
     9961 tgggctgtct ttgaaacggt tggcgtaacg ccctccgatt tcgatgacgg attcgcagtt
    10021 ccccatacgc gccctgcttt gcagcaacag gtacatactg cgttgcgcga tgctgccgcc
    10081 gtcgcctccg tccgcgcctt tcaacagggc ggcagcggca gaaaacctgc cgcttttata
    10141 gtgtttgagc gcctgattgt agaggttttg tgcggtttca acggtatgtg cggatgcgct
    10201 gccgccttcg gtattgaggt aatgctcttt caacttgcgg tcatcgagtt tttggacgta
    10261 tgtcctgccg gaagggtgtg tttttgcgtg ttccagtgct ttgactttgc cgtttaaggt
    10321 ttccacttcg ttcgacagcc ggacgatttt gccttccaga tagtccaaac ggtcttgcag
    10381 agtgggaacg ggatagggaa tagcgtccga actacctgcc tctgcctgcg gttcgggccg
    10441 catatcctga atattgcggg aaacagggga agaacaggcg gcggacacag acagccaaat
    10501 gataaaaagc ggtaatttgg tcttcattat tttttcagaa gcagggtcaa accgtcgccg
    10561 acgggcaggg tgatggggac gatgcgcggg tcgttcggca ggttttgatt aaaatctttg
    10621 aggatgccga cgctgggcgg cgcatcggaa gccgcttcgc gcatcaccct tccgttcaac
    10681 aaaatattgt cgatggcaat gatgccgcct tgccggacaa gcttgaggca acgctcgaaa
    10741 tattgcggcg tgggcggttt gtctgcgtct atcagtgcca aatcgtagct tccggcttca
    10801 ccctgtgcaa tcaaatcatc caatgtcagc aatgcgggtt gcagatgcag gctgatttta
    10861 tgtgccacgc ctgcctcgtt ccaaacctga cgcgccgtat cggtaaaggt tacattgatg
    10921 tcgcaggcgg taatccgccc gtgttttggc aacgccaatg caagcacggt gctgctgtat
    10981 ccggtaaata cgccgacttc cagatatttt tccgcgcgaa tcagctttgc cagccaaacc
    11041 aaaaccgccg cctgttcgcg cgcaatcgcc attttgccca tacggtgatg ctcggtcttc
    11101 tcacgcagcc gcgtcaaaac gggatgttcg ggttcgccga tggcgttcaa atagttttgc
    11161 aggtccggtg cgacattgga cagatgggtc gtcatttcgg tgggttcagt cttggtaata
    11221 ggtataaggt tttttcgcta cttttgccgc ctcgaagttt tcctgttctt cgggattgag
    11281 ttcgacatcc cacaaaagcc cccggttttc caaacgctgc tgttccaact caggtttttc
    11341 ttcaatcaga cggttgagga attgtgtggc ttcggattgg tagtgataca tctttgcgct
    11401 ccaattttac gggatacggc gtgattatac tggtattttc caaacgggat aaacggcttt
    11461 tatcaagaat acaggcagaa aagtaagggg ttttattata gaataaagcg tttttgcaac
    11521 ggaagcctac cttatgccac gcattgccgc cctgcccgac catcttgtca accaaatcgc
    11581 cgccggcgaa gtggtcgaac gccccgccaa cgccttaaaa gaaatcgttg aaaacagcat
    11641 agacgcaggc gcgacggcga ttgatgtcga actggcgggc ggcggcatcc gtctcatccg
    11701 cgtcagcgac aacggcagcg gtatccatcc agacgacatc gggcttgcgc tccaccgcca
    11761 cgccaccagc aaaatcaaaa ctctaaacga tttggaacac gttgccagca tgggctttcg
    11821 cggcgaaggt ttggccagta tcgcctcagt cagccgccta accctgacca gccgtcaaga
    11881 agacagttcg cacgcgaccc aagtcaaagc agaagacgga aaactcagca gccctacggc
    11941 agccgcccac cccgtcggca ccaccatcga agccgccgaa ctcttcttca atacccctgc
    12001 gcggcgcaag ttcctcaaat ccgaaaacac cgaatacgcc cactgcacca ccatgctcga
    12061 acgccttgca ctggcgcatc ctcacattgc cttctcactc aaacgcgacg gcaaacaagt
    12121 attcaaactc ccaacccaaa gcttgcctga acggattgcc gccattgtcg gcgatgattt
    12181 tcagacggct tcattggaaa ttgacagcgg caatggcgca ttgcggctct atggcgcaat
    12241 tgccaagccg acttttgcca aaggcaaaac tgacaaacaa tactgcttcg tcaaccatcg
    12301 ttttgtccgc gacaaagtga tgctccacgc cgtcaaacag gcataccgcg acgtattgca
    12361 caacgccctt acccctgctt tcgtcctctt tctcgacctg ccgcccgaag ccgtggatgt
    12421 caacgtccac ccgaccaaaa ccgaaatccg cttccgcgac agccggcagg tgcaccaact
    12481 cgtgttccac acgctcaaca aagcccttgc cgacacacgc gccgacttga ccgaaagcgt
    12541 cagcaacgca ggcgaagtgt tgcatgacat taccggcgtt acgcctgccc caatgccgtc
    12601 tgaaaacgac agcgaaaatc tgtttgatag cgcatccgac tacccgacag gcaacaaacc
    12661 cgatacacgc aatgcctttg gtgcatcagg caaaaccgcg cccatgcctt accaagccgc
    12721 gcgtgcgccg caacaacaca gcctgtccct gcgcgaaagc cgcgcggcca tgaatactta
    12781 cgccgagctt tacaaaaaaa ctgacgacat cgaccttgag ttgagccgat tagagcaggc
    12841 acgtttcggc aatatgccgt ctgaaacgcc tgctccccaa acagatacgc cgctttcaga
    12901 cggcatccca tcccaatccg aactaccgcc gctcggtttt gccattgccc aattactagg
    12961 catctacatt cttgcccaag ccgaagacag cctgttgctc atcgatatgc acgccgccgc
    13021 cgaacgcgtc aactacgaaa aaatgaaacg ccaacgtcag gaaaacggca aactacaaag
    13081 ccaacgcctg cttattcccg taaccttcgc cacgtcccac gaagaatgcg ccgccctctc
    13141 cgaccatgcc gatgcactgg caggcttcgg cttggaattg tccgacatgg gcggcaatac
    13201 ccttgccgtc cgcgcagttc ccgccatgct aggtaaagcc gacgtcgttt cactggccaa
    13261 agatgtattg agcgaacttg cccaagtcgg cagcagccaa accatcgaag aacacgaaaa
    13321 ccgcatcctc gccactatgt cctgccacgg ctcgatccgc gccggccgcc ggctcaccct
    13381 gcccgaaatg aacgcccttc tgcgcgatat ggaaaatacg ccgcgcagca accagtgcaa
    13441 ccacggcagg ccgacttggg tcaaactgac tttgaaagaa ttggacgcac tgttcttgcg
    13501 cggacagtaa gccgaaagtg ctagaatacg ccgcccgaga ccgccgttca gacggcattc
    13561 cgacgcaccg acagaaacat cacgaccgaa accaagagaa aaacatggcc tatcaagttc
    13621 tcgcccgaaa atggcggccc aaaacctttt ccgacctagt cggtcaggaa cacgtcgtca
    13681 aagccctgca aaatgccttg gacgaaggca ggctgcacca cgcctacctg ctgaccggta
    13741 cgcgcggcgt gggcaaaacc accatcgccc gcatccttgc caaaagcctc aactgcgaaa
    13801 acgcgcaaca cggcgaacct tgcggcgtat gcgaaagctg tacgcagatc gatgccggac
    13861 gctatgtcga cctgctcgaa atcgacgccg cctccaacac aggcatcgac aacatccgcg
    13921 aagtcttgga aaacgcccaa tacgcgccga ccgccggcaa atacaaagtc tatatcatcg
    13981 acgaagtgca tatgctttcc aaaagcgcgt tcaacgctat gctcaaaacg ctggaagagc
    14041 cgcccgaaca cgtcaaattc atccttgcca cgaccgatcc gcacaaagtc cccgttaccg
    14101 tattaagccg ctgcctgcaa ttcgtcttac gcaatatgac cgcgcagcag gttgccgacc
    14161 accttgccca cgtcctcgac agcgaaaaaa tcgcctacgc cccccccgcc ctgcaacttt
    14221 tgggacgcgc cgccgccgga tcgatgcgcg atgccttgag cctgctcgac caagccatcg
    14281 ccatgggttc gggcagcgtt gccgaacaag acgtccgcca aatgatcggt gcggtcgata
    14341 aacaatacct gtacgaactg ctgacaggca tcgtcaacca agacggcgaa gccctgctgg
    14401 caaaagcaca ggaaatggct gcgtgtgccg tcggctttga caacgccttg ggcgaacttg
    14461 ccatactgct gcaacacctc gccctgatac aggcagtgcc gaatgccttg gcgcacgacg
    14521 accccgattc cgatattttg caccgcctcg cccaaaccat aagcggcgaa caaatccagc
    14581 tttactacca aatcgccgtc cacggcaaac gcgacctgag cctcgccccc gacgaatacg
    14641 ccggctttat gatgaccctg ctgcgtatgc tggcgttcgc acccttggcg gcaacatcgt
    14701 gtgatgcaaa cgccgtgatt gaaaataccg aacttcaatc cccatcggca caaaccgccg
    14761 aaaaggaaac cgccgcaaaa aagccccaac cgcgccctga aacggaaacc gcccaaacat
    14821 ccgttcagac ggcatccgca gcggcaatgc cgtctgaaag caaaactgcc gaacccgttt
    14881 ccaatcaaga aaacaacgat gttccgcctt gggaagacgc gccggacgaa gcccaaaccg
    14941 cagccggcac ggcgcaaacg tcggcaaaaa gcattcagac ggcatccgaa gccgaaacgc
    15001 cgcccgaaaa ccaagtttcc aagaacaagg cagccgacaa cgaaaccaat gcccccttgt
    15061 ccgaaacgcc gtctgaaaac cccattcagg caacaccgaa taatgaagcc gttgaaacgg
    15121 aaacatttgc acacgaagcc cctgcagaac ctttctacgg ttacggcttt ccggataatg
    15181 actgccccgt agaagacaac gcagaaatcc cgccgcccga ttgggaacac gccgcccctg
    15241 ccgatgcgga agaagaaaac aacgccgacg aaggcagcaa cgacgaaaac aacacgccgt
    15301 ccgccccgcc gcccgaattt tccaccgaaa actgggcagc catcgtccgg catttcgccc
    15361 gcaaactcgg cgcggcgcaa atgccggcgc aacactccgc gtggacggaa taccatcccg
    15421 acaccggcct gatggttttg gcgatgaccg ccgaagcccg cgccaccgcc gacaaaaaac
    15481 gcctcgacaa aatccgcgac acccttgccc aagcctacgg gctgcaactc accctgcaaa
    15541 cccaagactg gcgcgacgaa gccggcaggg aaacccccgc gatgcaggac aagcgcgtcc
    15601 aagccgaaga caggcaaaaa gcacaagcat tgctcgaagc cgaccccgcc gcacaaaaaa
    15661 tcctccaagc attcggcgcg caatggcagc ccgaatcact ggaattggca gcaaaccggc
    15721 cgtaaacaga tataatgccg cccgaaccct tcggacggca ttgccgtttc cctcattcaa
    15781 tcaaaacaga caggagtatt cagtatgttc ggaaaagccg gattaggcgg cctgatgaaa
    15841 caggcgcagc aaatgcagga aaatatgaaa aaagcgcaag ccaaacttgc cgaaaccgaa
    15901 atcgaaggcg aagcaggcaa cggcctggtc aaaatcacaa tgacctgcgc gcacgaagta
    15961 cgcaaaatcg acatcagccc cgatttgatt caagaagccg ccgacgacaa agaaatgctc
    16021 gaagacctca tcctcgccgc cctcaaatcc gcccggggca aagccgaaga aaccgcaaac
    16081 aaaacaatgg gcgcattcac gcaaggtcta ccccccggag tgggcgactt cttccgctga
    16141 tccccgaccg tcattcccac gcaggcggga atctagaacg tagaatctga gaaaccgttt
    16201 tactcgataa atttccgtgc cgaggggtct ggattcccgc cttcgcggga atgacggcat
    16261 cagtttgcaa gattcggcgt gaacggtaaa aacagtgaga atgataagaa cgcaaaaacg
    16321 gcaagaatag cgggaatcgg caggctgaag cccaccctac cattatttac acatccgtac
    16381 cgcttaaatg ccgtctgaaa cttcgtcatt cccgtgaaaa cgggaatcca accccgtggg
    16441 agcagaaact tacaccccgt cattcccgcg aacgcgggaa tccagtaacc gaaaaaccac
    16501 aggaatctat cggaaaaaca gaaaccctcg accgtcattc ccgcgaacgc gggaatccag
    16561 taaccgaaaa accacgggaa tctatcggaa aaaacagaaa ccctcgaccg tcattcccgc
    16621 gaacgcggga atctagaacg tagaatctga gaaaccgttt tactcgataa atttccgtgc
    16681 cgacaggttt ggattcccgc cttcgcggga atgacggcat attttttgca tttgatataa
    16741 aggggcgttt gaattttgtt cagcaagtgc aaagtgttgc acataaaaag gcgcaggctt
    16801 catccgtaaa gtcggcgcag gatagaggca aagcgggcgt aggtcgggct gtagcaactg
    16861 tatttttcac cccgtcgggc aaaaatacca aaactcaaat caagccgttc ggaggcggct
    16921 caaaaaaacg gtacttcgca gcagaagtac cgtttatcgg gatttcaggt tttattcttc
    16981 ggggcgttcg ccgtcggttt cgtcctgcgt cccttcggtg atgtgcattt ctacgccgtt
    17041 gagggcgcgg atttttgcgt cgatttcatt ggcgacttcg ggattttcct tcagccagac
    17101 gcggacgttg tctttgccct gaccgatttt cgcgccgttg tagctgtacc acgcgccgga
    17161 tttgttgatg atgtcgtttt tcacgccgat gtcgatcaat tcgccttccc aactgatgcc
    17221 ttctccgtag aggatgtcaa actctgcctg acggaacggg ggggcgactt tgtttttgat
    17281 gactttgacg cgggtttcgt tgcccaatac ctcttcgcct tttttgatgg atccggtgcg
    17341 gcggatgtcg aggcggacgg aagaatagaa tttcagcgcg ttgccgccgg tggtggtttc
    17401 ggggctgccg aacattacgc cgatcttcat ccggatttgg ttgatgaaca caaccagcgt
    17461 gttggttttt ttgatgtgtc cggtcagttt gcgcaaagcc tggctcatca ggcgcgcctg
    17521 cagtccgaca tggctgtccc ccatatcgcc ttcgatttcg gctttgggga cgagtgcggc
    17581 tacggaatcg acgactacca tatctatgcc gcccgaacgg acgagtgtgt cgcagatttc
    17641 caaagcctgt tcgccggtat cgggctggga caggtaaagc tcttcgactt ttacgccgag
    17701 tttgcgggcg taaacgggat caaaggcgtg ttcggcatcg acaaaggcgc acacgccgcc
    17761 gtttttctgg cattgggcga cggcttcgag gcagagggtg gttttgccgg aggattcggg
    17821 gccgaagatt tcgacgatgc gcccgcgcgg cagaccgccg actccgaggg cgaggtctaa
    17881 tccgagcgat ccggtggaaa tgacttcgag gttttcttcc tgctggctgc cgtccatttt
    17941 catgatggcg cctttgccga aacttttttc gatttgcgcc agtgcggcgg caagggcttt
    18001 gcttttgtcg tctgacattg gggttactcc ggaacaaatg cggtatgtgg gatgcggcgc
    18061 aacacgggct gcggcgcggg atgtgtatcg ttttcccgat gtgcgggcta tcggtaatgc
    18121 tgcttcacgg ggttgccatt atcgcatatt tccttgcttg ccgatatgcg gcaggacgcg
    18181 gcggcttgtg ccggaatgga atctggatgc cgtctaaaag gcggccggct ttgttataat
    18241 ggcggctgtt ttttctgtgt gtgcctgttt tatgtgttcc tgccttgttg tcaaaaatac
    18301 cgttatcgga agcggacgca ccaaaatcgc cgtgccgctt gtcgcccgcg atgctgccga
    18361 actttccgcc gtacttgagc aaatcaaaaa tatgcccttc gatattgcgg agttccgcgc
    18421 cgactttttg gaatgcgcgg gcagcatcgg cgaagtattg cgccacacgc agaccgtccg
    18481 cgacgcgctg cccgacaaac cgctgctgtt tacgttcaga cggcatggcg aaggcggctc
    18541 gttcccgtgt tcggacgatt attattttga actgctcgac gcgctgatcg aaagccgcct
    18601 gcccgacatc atcgacatcg agctgttttc cggcgaaacc gccgtccggc gcgccgtggc
    18661 aaatgctcaa aaaaacggca tcgccgccct actctgcaat catgagtttc accgcacgcc
    18721 gccgcaagaa gaaatcgtat gccgtctgaa acagatggag gactgcggcg cggacatctg
    18781 caaaattgcg gtgatgccgc aaagcgcgga agatgtgctg actttgcttt ccgccacgct
    18841 cgaagcgaaa cggcttgccg ccaaaccgat tgttacaatg tcgatgggac agacggggac
    18901 ggtcagccgg cttgccggac aggtgttcgg ctcaagcatc acgttcggtt cgggaacgca
    18961 aaactccgcg ccggggcaaa tcggcgtatc cgccctccgt gcggcactcg actgtctcga
    19021 aagcggcgca gactgatttc agacggcatc aaaacatgat gaaactcaat ccccaacagc
    19081 tcgaagccgt ccgttacctc ggcggccccc tgctcgtcct tgccggtgca ggcagcggca
    19141 aaaccggcgt aattactcaa aaaattaagc atttgattgt caatgtcggc tatctgccgc
    19201 ataccgtcgc cgcaattacc tttaccaaca aagccgctgc ggaaatgcag gagcgcgtcg
    19261 ccgaaatgct gcccaagtcg caaacgcgcg ggctgacaat ttgcacgttc cattctttgg
    19321 gcatgaagat tttgcgcgaa gaggcgaacc atatcggtta caaaaaaaac ttttccatcc
    19381 tcgattccac cgacagcgcg aaaatcatcg gcgaactttt gggcggtacg ggcaaagaag
    19441 ccgtattcaa ggcgcagcac cagatttcct tgtggaaaaa cgatttaaaa acgcctgaag
    19501 atgtcgttca gacggcctct aatgcgtggg aacaacaaac cgcacgcgtg tatgcgagct
    19561 atcaggaaac cttacaaagc tatcaggcag tggacttcga tgacttaatc cgcctgcctg
    19621 ccgtgctgtt gcagcaaaac agcgaagtgc gcaacaaatg gcagcggcgg ctgcgttatc
    19681 tgctggttga cgaatgccaa gatacgaata cctgccaatt tgcgctgatg aaactcttga
    19741 ccggtgcgga aggtatgttt accgccgtcg gcgacgacga ccagtccatc tacgcgtggc
    19801 gcggtgcgaa tatggaaaac ctgcgcaaaa tgcaggaaaa ctatccgcag atgaaggtcg
    19861 tcaaactgga gcaaaactac cgctccaccg cgcggattct taaaatcgcc aacaaagtca
    19921 tcgaaaacaa ccccaagctg tttaccaaaa aactttggtc gcaattgggc gaaggcgagc
    19981 cggtcaaagt cgttgcctgc caaaacgagc aacacgaagc cgactgggtc gtcagccaaa
    20041 tcatcaaaca aaaactcatc ggcggcgaca aaacccaata cgccgatttc gccgtgttat
    20101 accgcggaaa gcatcaggcg aggattttcg aggaagcatt gcgcggcgtg cgcatcccct
    20161 accggctctc cggcggacaa agctttttcg acaaagccga aatcaaagac gtgttgtctt
    20221 atgtgcggct gcttgccaac cccaacgacg atcccgcctt tctgcgcgcc gtaaccacgc
    20281 ccaaacgcgg catcggcgat gtaacgctgg gcaagctcaa cgcctacgcg cacgaacacg
    20341 aatgcagcct gtatgaagcc gcgcaaaccg aagaagccct tgccacgctg aacaatacca
    20401 accgccaaca cctgcaagcc tttatggata tgttcggcag ctaccgcgcc aaagccgaaa
    20461 caggcgaagc aggcgagttc atcaacagcc tgctcgaaga aatcgactat gaaaaccacc
    20521 tgcttgccag tgaagaaggc aaagctggcg aaatcaaatg gcgcaacgtc ggcgatttgg
    20581 tatcatggtt tgcgcgaaaa ggcggggaag acggcaaaaa catcatcgaa ctcgcccaaa
    20641 ccgtcgcctt gatgacgctt ttggaaggaa aagacgaaga agaaaccgat gccgtctcgc
    20701 tatccacgct acacgccgcc aaaggtttgg agtatcccta tgttttcctt gtcggttgcg
    20761 aagaaggtgt tttgccgcac aacgacagca tcgaagaagg caacgtcgaa gaagaacgcc
    20821 gcctgatgta cgtcggcatc acccgcgcca aacgccagct tacgctgacc cactgcgtca
    20881 aacgcaaaaa acaaggcata tggcagttcc ccgaacccag ccgattcata gacgaaatgc
    20941 cgcaggaaga tttgaaaatc ctggggcgca aaggcggcga accgattgtc agcaaagaag
    21001 aaggcagacg caaccttgcc gatataatcg gaaggctcga cagcatgaaa aaaggcggtc
    21061 cggaggatta aaccaagata gggaaacgaa gccggagaca atgtatagtg
//
LOCUS       DAXHMX010000018        20688 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHMX010000018 DAXHMX010000000
VERSION     DAXHMX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20688
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.018"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(63..239)
                     /locus_tag="ACFM4S_000503"
     CDS             complement(63..239)
                     /locus_tag="ACFM4S_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050243.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(242..1600)
                     /gene="murF"
                     /locus_tag="ACFM4S_000504"
     CDS             complement(242..1600)
                     /gene="murF"
                     /locus_tag="ACFM4S_000504"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6050244.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQTLAKVWRENVNPF
                     VFGITGSVGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1725..2960)
                     /locus_tag="ACFM4S_000505"
     CDS             complement(1725..2960)
                     /locus_tag="ACFM4S_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6050245.1"
                     /translation="MYRGGVFADVKNYKRRSKIWLTILLTLILSCAVMDKIASDKDLR
                     EPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKKASTWHIRHVAPKYKNYVVVIG
                     ESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAHATNLSLPQTLGLPGEPNNNIV
                     SLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPWFTQRGDYGKSAGLSDRLLLPA
                     FKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARRFQYQTEKISCYVSTIAQTDKF
                     LEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLTHGAWKRQSYGVPLVKISSDDT
                     RREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFWGNVPDVPGEGNNLAFIDGLPD
                     DPAPWYAGKGKSTKNTSKK"
     gene            complement(2935..3372)
                     /locus_tag="ACFM4S_000506"
     CDS             complement(2935..3372)
                     /locus_tag="ACFM4S_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050246.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGGIC"
     gene            complement(3395..4873)
                     /locus_tag="ACFM4S_000507"
     CDS             complement(3395..4873)
                     /locus_tag="ACFM4S_000507"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6050247.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFQTPWGEGKCHTRLLGRFNAQNLAACVALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4898..6643)
                     /locus_tag="ACFM4S_000508"
     CDS             complement(4898..6643)
                     /locus_tag="ACFM4S_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6050248.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="ACFM4S_000509"
     CDS             complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="ACFM4S_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6050249.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="ACFM4S_000510"
     CDS             complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="ACFM4S_000510"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6050250.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIAGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="ACFM4S_000511"
     CDS             complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="ACFM4S_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6050251.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8708..9898
                     /locus_tag="ACFM4S_000512"
     CDS             8708..9898
                     /locus_tag="ACFM4S_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6050252.1"
                     /translation="MSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLIAEKIGKHGN
                     WIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQLQELLSQT
                     AGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAPEASQKVV
                     HLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSARPLHDLQ
                     LSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQYYHPQRN
                     QGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQSHFLLNL
                     GITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVDWAGFRFGDI
                     CHKL"
     gene            10030..10106
                     /locus_tag="ACFM4S_000513"
     tRNA            10030..10106
                     /locus_tag="ACFM4S_000513"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10064..10066,aa:Met,seq:cat)
     gene            10114..10189
                     /locus_tag="ACFM4S_000514"
     tRNA            10114..10189
                     /locus_tag="ACFM4S_000514"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10147..10149,aa:Ala,seq:ggc)
     gene            complement(10480..11301)
                     /locus_tag="ACFM4S_000515"
     CDS             complement(10480..11301)
                     /locus_tag="ACFM4S_000515"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6050253.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(11334..11978)
                     /locus_tag="ACFM4S_000516"
     CDS             complement(11334..11978)
                     /locus_tag="ACFM4S_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6050254.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11981..12826)
                     /gene="ftsN"
                     /locus_tag="ACFM4S_000517"
     CDS             complement(11981..12826)
                     /gene="ftsN"
                     /locus_tag="ACFM4S_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6050255.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRVKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12932..14428)
                     /locus_tag="ACFM4S_000518"
     CDS             complement(12932..14428)
                     /locus_tag="ACFM4S_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6050256.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLVQTDCQVPQMPSENTKLLDLVDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSVSVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14442..14762)
                     /locus_tag="ACFM4S_000519"
     CDS             complement(14442..14762)
                     /locus_tag="ACFM4S_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6050257.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15245..16771
                     /gene="putP"
                     /locus_tag="ACFM4S_000520"
     CDS             15245..16771
                     /gene="putP"
                     /locus_tag="ACFM4S_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6050258.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWI
                     KKPAQAAGESGLLTMYEIVPGFIVCLIIAVLVSLFNKEPSREIQKRFEKADADYRAAR
                     "
     gene            complement(16861..17028)
                     /locus_tag="ACFM4S_000521"
     CDS             complement(16861..17028)
                     /locus_tag="ACFM4S_000521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050259.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17020..20625
                     /gene="putA"
                     /locus_tag="ACFM4S_000522"
     CDS             17020..20625
                     /gene="putA"
                     /locus_tag="ACFM4S_000522"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6050260.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4852 a         5962 c         5051 g         4823 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagagacttt cccaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gagcatacgc gccgacttcg gcgtgcatgg
      481 cggcggcttc gtcctcaccc agttcgccca tatcgcccat cacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcggctt tcatgctgtc agggttcgca ttataagtat
      601 cgtcaatcag ggttgcgccc ttgattccgg atttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag tgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgacca atgcggcatc gggtttggcg atttgtgtca
     1081 aaaccgccag ttcgccaaaa tggttcatac ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccagcatc tccttcaccg
     1261 tcgtcttgcc gaccgaaccg gtaatgccga acacaaacgg attcacattt tcacgccaca
     1321 cctttgccaa cgtttgcaat gcggcaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcatcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttaggtcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt tttttatcgc ctgttttgct gtggtaaaac
     1681 acagattatt ttcccattct cattcggcat tttttctgta cgtatcattt tttagacgta
     1741 tttttagtcg atttgccttt tcccgcatac cacggcgcgg ggtcgtcggg cagtccgtcg
     1801 ataaaggcaa ggttattgcc ttcgcccggc acatcgggaa cattccccca aaaatcatag
     1861 ccgtcatcgg gcaactcgtc ggtttcgata cccgtccaac tgccgaatcc gcgtaaaaaa
     1921 ttaaacgcgc tgcgcctcac tttaatcatt tcgcgccgcg tgtcatcgga cgaaatttta
     1981 accagcggca cgccgtagct ttgacgcttc cacgcaccat gtgtcaacgt tcgctcgccg
     2041 cctttaccga catgcatcaa accgtggtcg gaaaagtaaa ccaaagacca gctttcttta
     2101 ttttcattca atatcttaac tgtatcttct aaaaatttat cggtttgcgc gatggtggaa
     2161 acatagcagg atattttttc agtttgatac tgaaaccgcc gcgcatcctt atccaaacgt
     2221 gtgcaaaaat cactgtgcga acccatcagg tgcatcacaa tcagccgagg cttcgtgcct
     2281 gcatttccta tcaaaacccg tttgaacgcc ggcaacaaaa ggcggtcgct caaccccgcg
     2341 cttttgccat aatcacccct ttgggtaaac cacggataat cgctgcgtag ggcataggtg
     2401 gaaatttcgt tggcaaaatg ccccaacatt ccttgattag acagccacgc cgtccgaaaa
     2461 cccgcctgct tcgccaagct gacgatgtta ttgttcggtt ctcccggcag ccccaaagtc
     2521 tgcggcagcg aaagattcgt cgcgtgggcg gtcgattggt aaccgtttat caacagccct
     2581 ttggtctgac tcaaaaaagg cgtatcgggc aatgggaaac cgtaaacatt catataatcc
     2641 gaacgcgcgc tctcaccgat aaccacaaca taatttttat acttgggcgc aacatgacgg
     2701 atatgccatg ttgacgcttt ttttgctgct tccaaaatgt gggcgcgctt ggcggcatat
     2761 tgtgccggcg cggaagccaa atcgtaatac aggtcgaaaa tattcaacaa caggccggca
     2821 tcaggttctc gcaaatcttt atcgctggcg attttatcca tcaccgcgca ggacaaaatc
     2881 aaagtcaata atatagtcag ccatattttg ctgcggcgtt tatagttttt tacgtcagca
     2941 aatacccccc cccgatacac aatatctcca aactgtcaag ccaaaaatga aaaataatgc
     3001 ctgcacaaaa taaagcgacc cgggaagatt gccgacaaat tcacgcgcct cggcaggatt
     3061 gctttccaat atcgaaccga ctatctgata agacggcgca ccatacagcc agccgaccgg
     3121 caaatatagg gcggttgtgc cgacgtaaat cagtaaaaca actgaagaaa cgcgcgggaa
     3181 accgcgtgcc aataataata aaaatacaac cgaagccaaa gcacccacgg cagaataacg
     3241 gtagccgtat tcatattcca agtgataacc tgatgcgatg gccgcgccaa aacaaaaggc
     3301 taaaagcgcg caaatgccca atgtattttt taatgtctga tccatatttt ttattcgggc
     3361 ggattgtttc atacgatgcc gtccgaatat cccattattt acgagttaac aaagcctgtc
     3421 cgacgatttc aagatcggaa aaacggtgct tcacgccttg tacatcctga tagttttcat
     3481 gccctttgcc ggcaatcagg atgatgtcgt ttgcggcggc ttgttcaacc gcataacgga
     3541 tggcggcggc acggtcggct tcgacgcatt cgggcgcggg aacggcaggc aggatgtcgt
     3601 tgatgatgtc gtgcggattt tccaagcgcg ggttgtcgct ggtgacgacg actttatccg
     3661 cgccctgtac ggctgccgcg cccatcagcg ggcgtttgcc gcgatcgcgg ttaccgccgc
     3721 aaccgaatac gcaccacaaa gccgcaccct gcggtttgat ttcctgcaag gtggcgagtg
     3781 ctttttccaa tgcgtcgggc gtgtgggcat aatcgacaac aaccaaaggt ttgccgctgt
     3841 tcataataca atccatacgg cctgaagcag gacggatttt ttccagtaca tccaatacct
     3901 tatccagcgg atagccgttg gcgcaaagca aagcgacaca ggcggcgaga ttttgcgcgt
     3961 tgaaccgtcc gagcaggcgc gtgtggcatt tcccttcgcc ccacggggtt tggaatacgg
     4021 cttctatgcc gtctgaagag gcggtaaagt cggtaatgcg gatgtcggcg tgttcgctga
     4081 agccgtaact gtaaacggcc aaatcggggc agtctttttt cagacgacct gcgagttccg
     4141 cgccgtattc gtcatctaca ttgataacgg cgtgtttcaa gccgtgccag taaaacaggc
     4201 gcgacttgat ggcaccgtag gcttccatcg tgccgtggta gtcgaggtgg tcgcgggtga
     4261 ggttggtaaa gattgcgctg cggaatgaca cgccgttgac gcgcgactgg tcaagcccgt
     4321 ggctggagac ttccatcgcg gcgactgttg cgccttgttg acggaaacgg tagagcaggg
     4381 tttggacatc gacgggggcg ggtgtggtat gcgtggtttc ttccaatgca ccccaaaagc
     4441 cgttgccgac cgtgccgaca atggcggttt tttcgcccaa caaatcggca gcttgcgcca
     4501 gccattgcgt gatggaggtt ttgccgttgg ttccggttac gccccagact ttgagaccgt
     4561 ctgaaacgtt gccgtaaact tgcgccgcca atatgccggc acggtgtttc aaatctttga
     4621 tgccttgatt ggggactttc cattcgggat tccacgcgaa tttgccatcg tcgtcccaaa
     4681 aaacaaaagc cgcaccgttg gcaacggcgg cggggatata actgcgtccg tcggcatatt
     4741 cgcccggaca ggcaacgaaa atatcgcctt gtttgatttg gcggctgtct gaatgcaaca
     4801 aacgccctgc cgcgtttgca cacgacaggg tcgggatgcc ggtttcagcc aaaggggtta
     4861 acttgctgaa cataaaacaa tctcgttgat actcggatta agacggtgtt ttgacggctg
     4921 cggcggtcag tggcttggtc ggggaaatgc ccaagatgtt caggctgccg cccataattt
     4981 ttttgaaggg cggccctgcc actacgccgc cgtaataacc gtgggcagtc ggttcgtcaa
     5041 tggttaccgc cacaatcaca cgggggtttt tggcgggggc aaaaccgata aaggtagcga
     5101 tgtgtttgtt gtcggcataa cgcccgttga cgaacttgcg cgccgtgccg gttttcgcgc
     5161 cgacatcgaa accgtccacc gcacccgccg taccggtgcc gcccggctcg gtcacggaaa
     5221 ccatcagatt gcgtacctcg cgcgcggtcg attctttgaa tatgcgtttg ccttgcggcg
     5281 caaccgcctg tttttcaaag ctgaccggca gcaaaacgcc gtcgtgcgtc agtgcggtat
     5341 aggcgcgcgc caattgcagc aggctcaatt gcaggccgta accgaaagac atcgtcgcct
     5401 gttcgatggg ccgccacctg cgccaatttc tcaacaaacc tgcagtttcg cccggaaagc
     5461 ccgagtgcat acgcacaccg atgcccaact catgatagaa gtcatacatt tcttcggcac
     5521 cgaaacgcgc agacagtttg cttgtgccga cgttggacga tttctgcatg atgccgcgca
     5581 catccaaaga ggggtaaaca tgggtatcgc gcacgggaga cggtccgatt ttataaggct
     5641 gcgtattcag ccgttcgttc aaatcggttt tgcccgcatc caatgctttc gcaatcacaa
     5701 acggtttgat tgccgaaccg ggttcgatca tatcggttac ggcacggttg cgccgctgtt
     5761 cgctgtctgc ccggccgggc ctgttgggat cgtaggcggg cgtattggcc aaggcgagga
     5821 tttcccccgt gcgggcatcc aaaaccacca ccgttccggc ttttgcctga tggtattcga
     5881 ccgccttgtt caactcttca taggccaagg tctgaatcct ctgatcgagg gaaaggatga
     5941 tgtctttgcc gtttttcggg gctttattgc gcggggagtc caagctgtcc acaatattgc
     6001 cctgccggtc ccgcaaaacg acttccgcgc cgtcttcgcc atgcaggctg tcttcaagcg
     6061 aaagttccaa accttcctga cctttgccgt caatatcggt aaatccgatg acgtgtgcaa
     6121 acaggttgcc catcgggtaa tggcgtttta attctttttc aaatacaaag ttttccaaac
     6181 ccaaggcttt gacctcttcg gcaaccttgg gatcgagctg ccgcttaatc cagataaacg
     6241 acttgccttt ctgttcgagc ttgttcctca aaacatcaac cggcacatcg acaagctcgg
     6301 acaggcgttc caattgtgcg gcagacggca tttccttcat ctctttaggc acggcaaaca
     6361 gggactccgt cggcgcactc aacgccaaaa ccgcaccgtt ccggtccgaa accgtaccgc
     6421 gtgtagccgg caatgtttga gtccgcacaa tccggttgtc gccctgttct ttcaaaaagt
     6481 tatacgttac cgtctgcaga tacagtccgc gggcaatcag accggcaaac aagaccgcta
     6541 ttgccatcag gacgaagctg atccgtccgt tactggtcat cggctttttg acctgctctt
     6601 ctttgggcag catccggggc ttatattcac tcttaatcaa catttttact tctcgttatt
     6661 attatcctga cgcaggaatc cgattccggc acacaggctg cttctatctt tgatgcgcca
     6721 ccataaaggt attgcccgaa accggcggat ggaggttttg tttttctgcc gccgccctga
     6781 tcgcttcgtg gttcgccaaa cgcgcctgtt gcagcctcat ttgcgcataa tcctgctcca
     6841 aggcgatttc ctgttttttc gccttatcca aagctgtgaa gttgagcctg tactggtttt
     6901 gctgcatcac aacggaaaaa gcggaaacgc acaccgcaag cagcagaagg aaattcaatt
     6961 tgttcattgc cattcagacg tttttctctg tgattgttcc ggtatcggac cggcagtccg
     7021 ctccgccaca cgcaaaaccg cacttctcgc cctcggattg gcggcaattt ccgcctcacc
     7081 cggctttaat gccctgcccg cgattttcag gggcggctcg ggcaaatccg cttccctgac
     7141 cgccgcccag cgcggcaggg gcgcgtgttg cgaatatttt ttgacaaact gcttcacaat
     7201 gcggtcttcc aacgaatgga aagcaatgac cgccaaacgt ccgccctctt tcagacgaca
     7261 catgacctgc ggcaatactg cccctacttc ttcaagctcg cggttaataa agatgcggat
     7321 tgcctggaag gtgcgcgtcg caggatcctg cccccgctcg cgagtacgga cgttttgtgc
     7381 cacgatctgc gccagcttgc gggttgtatc gattggactt tccgcccgtt gcgcaacaat
     7441 ggcgcgcgca atctggcggc taaaccgctc ttcaccataa ttcttgatta cctcgtgcaa
     7501 atcctgttcc gacgcaaccg ctatccactc tgcggcagac ataccgcgcg tcgtatccat
     7561 acgcatatcc aaaggggcat cgaaacggaa gctgaaaccg cggctgccgt catcgatttg
     7621 cggggacgaa atccccaaat caaacagcgc accgtccacc ttgccgatac ccaaaccgtc
     7681 caatgccgtc tgaaacgaag caaaaccgcc atgcacgaca ccgacccgtt tgtccgaacg
     7741 cgccagctct tctgccacag caatcgcctg cggatctttg tcgaaaacaa tcaaccgccc
     7801 cgcatcgccc aaacgcgaca aaatcagccg ggaatgccct cccctgccga acgtaccgtc
     7861 cacatagaca ccgtcttcgc gcacggcaag cgcatccacc gcctcattca gcaagaccgt
     7921 gatatgccgg taactttctg ctccactcac aattgcaaat ccgtctgacc caactggaag
     7981 gcaagttcgt caggatcgtc atccaaagcc tgaaccatct cagcctccca ctgctcgcga
     8041 ccccaaagct ccaaacggtt ggcacgaccg accaaaacga cttcacggtc gaaatccacc
     8101 cttttcctca gtccggcagg aagcagcacc cggccggcgc tgtcccattc caaaatttcc
     8161 gcgttatgca gcaaaagatt ttgaaaccgc cgcaaaacag ggttatccgc cacttttaag
     8221 tttaaaagtt gcgccgcaac cttttcccac tccgcaacag ggtacatcaa cagcttgtgt
     8281 ttcgactcga gcgttaccac tacggcaggc gtatagaggc gcgacagaat gtcacggaat
     8341 ttggcaggaa cagccaaccg ccccttactg tcgatgctta attcgtgtgc gccgccgaac
     8401 atgacatgtc ccaagccgaa atcaaaatca caagggtaaa agagacactt tgccccacaa
     8461 ttccccacca atcgacacta taagaaattt taaacactcg gtcaaatcag ggcatgaaaa
     8521 ctcattaaca tatctgaaat ttttattcct tttaaaaaca ataagataaa aaatgacgac
     8581 aacggcacgg cgggtgcggt acagaataat cgaaccaata aacaactata tatgattaat
     8641 ttaataatat aaacacaata tatagtatta agataaagcc atgacagcac ccgtaccaac
     8701 gtgtaatatg tcgggaaatc caataaattt acacaagcta acacttatca tgcccctccc
     8761 tctcccctct cccgaagcac ggcaattctc gctcaaactg caaaccctca ttgccgaaaa
     8821 aatcggcaaa cacggcaact ggattccatt ttcacgtttt atggaattgg ttttatacgc
     8881 tccgcaatac ggctactaca ccggcggcag ccataaaatc ggcaataccg gggattttat
     8941 taccgcaccg accctcacct ctctgtttgc acagacactg gcacgccaac ttcaagaact
     9001 tctatcccaa acggcgggca atatctatga attcggcgcg ggaaccggac agcttgccgc
     9061 cgatttgttg ggcagcattt cggacggcat cagccgttac tatattattg aaatatcgcc
     9121 ggagctggca gcacgtcaga aaaacctgat tcaagcacgc gcaccggaag catctcaaaa
     9181 agttgtccac ttgaccgcac ttcccgaagc gtttgacggc atcatcatcg gcaacgaagt
     9241 actcgatgcc atgcctgtcg aaatcgtccg taaaaatgaa ggcggctcat tcgagcatgt
     9301 cggcgtttgc ctagataatg accgttttac ctattcggca cgaccgctgc acgacttaca
     9361 gctatctgcc ttggcttccc tctattttcc tcaaacagat tatccctaca ccagcgaact
     9421 acatccgcaa caatatgcct ttatccgcac ccttgcctca aggcttgaac gcggaggtat
     9481 gatattcatc gactacggtt ttgatgcagc gcagtattac caccctcaac gcaatcaagg
     9541 tactctgatc ggacactacc gacatcacgt tatccacaat ccttttgact tcatcgggtt
     9601 ggccgacctg accgcgcatg tcaactttac cgacattgcc caagccggta cggatgcagg
     9661 actggatttg acaggctatc tgccccagtc ccattttctg ctgaatctgg gcataaccga
     9721 actgttggca caagtgggaa aaacagactc gcccgcctac atccgtgaag ctgctgccgt
     9781 tcagaaactg attgaccagc atgaaatggg agaattgttt aaagtcatcg cattcggtaa
     9841 aaatatcggc gtcgactggg caggattccg cttcggcgac atctgccaca aactctaacc
     9901 ctcatgccgc ctgaatccgc ttcagacgac ataaactttt taacatttaa aaacagtcaa
     9961 ctaattcaaa attaaaaaat acggcttgtc aaaaaaacag aaaaacatat aatagtgtct
    10021 tcacgaaacg gcgaattagc tcagtcggtt agagcagagg aatcataatc cttgtgtccg
    10081 gggttcgaat ccctgattcg ccaccaaatt ttcgggggta tagctcagtt ggtagagcgc
    10141 ttgcatggca tgcaagaggt cagcggttcg atcccgctta cctccaccag ataaaaaagc
    10201 acagaccgta aaaaggtatg tgctttttta ttacctgatt gccgggaaat aaagaacaaa
    10261 ccactgcctt caaaacaggc aatcgacttt aaacctatcg ccccgcctgt cctgatttta
    10321 tagtgaatta aatttaaacc ggtacagcgt tggctcgcct tgccgtacta tctgtactgt
    10381 ctgcggcttc gtcgccttgt cctgattttt gttaattcac tatatcagcc cgccagacaa
    10441 acccgacccg aataatgtct tcaggtcggg tttatggttt cattcccaac ttatccagcc
    10501 cgacaaccac aatattatga tgacgatgcc gaaaacaatg cggtaatagg caaacgggat
    10561 atagtttttc ttggaaacaa acttcagcag tgctttgacc gctactaaac cggaaacaaa
    10621 agcggcaata aagcctatca gaatcaaacc gacatcatgc agggtgaaaa accggtaatg
    10681 tttcaggaca tcataagccg ttgcggcaac catcatcgga acggccaaga aaaatgaaaa
    10741 ctccgttgcc gttttccgct cgattcccca aagcatcccg cccataatcg tactacccga
    10801 acgggacgta cccggaacca gtgcaaacac ttgggcaacg ccgatcatca acgcatcaat
    10861 cggacgcaat gcatcaacat cggcaatttt aggctctgcc cggctttggc gtttctccac
    10921 ccacaaaata aaaaaaccgc ccaaaaccag catgaccgcc acactcaaag gattgaacag
    10981 atgttcttta atctgtttgc cgaacaacag ccccatcacg gcggcaggta taaaagcaat
    11041 ggcaagattg aggacgaagc ggttggcttt ccggtctttt cccacgccat gcaacacatt
    11101 gctgaaacgc tgccggtatt caaacactac cgccaaaact gcaccgagct ggatggcaat
    11161 ttcaaaaacc ttgtgattgc tgtgaaaatc aatcagattg ccgaacacaa tcaaatgtcc
    11221 ggtgctggaa atcggtaaaa attcggtaaa accttctacc aagcccatca tcagggcttt
    11281 caggacaatc agaaaatcca ttgcttgcgc ttctttcgga tacgggagtt cggctatttc
    11341 tgtacagcag gggtctgacg cttgcgttct tccctgactt tggcaaccaa ttctttaatc
    11401 gtatgaacgc cgccgtcaaa gccattattg aagataacgc ggtattttcc gccgacaata
    11461 acggtcggcg tgctgtcaat accatattgt tccgtcagtt tctgcatttt taatgcggcg
    11521 gcggcagctt cgggggaatc ataggcgcgc atcagttttt tgccgtcaaa gcctttttga
    11581 gacaaagccc attttccggc aacagccctg ttttccaagc ggattttttg ttcgtaaact
    11641 gctttaaaca cagcagggtt tgcctgatat ttcaaacccg acaaattgac ggcagccgcc
    11701 atcctggcca gaccgagcat ttcaggctgc cagaccacgt gctccgtcct caaataggca
    11761 tcagacggca atgccttgcc caatttcaat aacaaaggat cgaaatgatg gcaatgtacg
    11821 cagaaatagc cgaaaaattc caaaacctca attttacccg actgttcttg aggaatgggt
    11881 ttatccaaca caagatagtc ttccccttcc gtcagggcat atgcctgcgc ggacaacact
    11941 gccgacagca gcagcggcaa cagatgtttg agcttcataa ttatttgctt tcgatagaac
    12001 ggatcaggct ggcgacttca tgttttttca actcgtcctg catttttttc accgcatcgg
    12061 cagacatatt gccgctttgc acccggtaaa gcgttttatg tcccgcctga taaccgacca
    12121 ccttggaaga tatgcccaag attgccagtt tgacacgctg cccttccgcg ctccggcggt
    12181 cggcatacgc gcccatttgc agataatgcg ttgcttccgc cttgtcggac gttttcattt
    12241 tctgcacttc tttggcggcg gcactgcgcg ctttttcgat gctgccgctg ttgaggattt
    12301 gttccggggt cggtttgggt gcaacttttt ccttttccgc ctttttctct tcttttggag
    12361 cttttttctc tgtttcttta gacggtttta ccgcttgttt tttaaccgtt tcggcatctt
    12421 tcttctgcgc tttttccctg acggtttgtt cacgctcttc cgtcagcact ttcttacgca
    12481 ctgcctgtcc gtccggctct tcccgttccg gctcgcccgc cttttcttca acctcgtcgg
    12541 ctttatcggc agctttttcc gcatccgact gctctgcctc tgtcgcagca tccggttcgg
    12601 acaaggcgtt ttgatcggcc ggttcaggtt ggatgtcttc cttaggctgg tttttcggtt
    12661 tcaggatttc cgtttctgca ggctgcttcg acggaaccgg gattttgaac gcattttgac
    12721 cgctctggtt cagataaaac aaaataccgg caataatgac cgtcgccagt atcaaaccga
    12781 agaaaaaacc ggacagacct tttccggatt gggaaaattt gttcataaac ataccttaat
    12841 gtgtttcaga cggcattaac gccgtttgct tacgggcgga tattctaaca atatcgccat
    12901 atttgggcaa aacctgcttc cattcccgtt cctataaagc gcgacggaaa cctatggctt
    12961 tcatgacgtg gcttctgccg acttcttcgt cgcccgccaa atccgccaat gtacgcgcca
    13021 cgcgcataat gcggtggaag ctgcgggcgg aaagggagag tttttccagc aggccgccca
    13081 atgcttcctg cgcttctttt tgaatgcggg cggatgtgtc gagttcactg acactcaagg
    13141 cggcattcac tttgccttgc cgtgcgtatt gtttgtcgcg ggcggcgata acgcgttcca
    13201 aaacggacac gctgctttca cccgcttcct gctgcatcag ttcggcggcg gacaggctgg
    13261 ggacttcgat ggtcaaatcg atgcggtcga gcagcgggcc ggaaattttg ctgcggtaac
    13321 gcgcgacgct ttcgggcgtg cagcggcagg gtttgacggg atgcccgaga tagccgcacg
    13381 ggcaggggtt catggcggcg acaagttgga atttggcggg atagacggct tggcgcgccg
    13441 ctcgggaaat gtggatttcg ccgttttcca acggttcgcg caaaacttcc aaaactttgc
    13501 ggtcgaactc gggcaattca tccaaaaata aaacgccgtg atgcgccaat gaaatttcgc
    13561 ccggacgcgg atccgaaccg ccgccgacca tagccgccgc gctggcgctg tgatgcggac
    13621 tgcggaaagg acggttgctg tcgagttgtt gctggtggtt gggcaggagc gaacgcaatg
    13681 cccaaacttc taccaattcg tcttcggtca gcggcggcag gatgccgggc agccgttggg
    13741 agagcataga cttgcccgtt cccggcggac ccatcatcaa aaggctgtgt ccgcctgcgg
    13801 cagcgatttc caaagcaagg cgggcagtgt gctgaccctt gacatcgacg aggtcgagca
    13861 atttggtgtt ttcagacggc atctgaggaa cttgacagtc ggtttgcacc aaaggttcga
    13921 taccgttcaa atgggcggcg acttcgccca aagagcgcgc gccgtaaacg gtaatgccgc
    13981 gcatcacggc ggcttgtcct gcgttttctt caggcaaaac aaatgcacgt tttgcctgca
    14041 taccctgcca cgccatcgcc aacgcgccac gcacggggcg caacagcccc gacagtgcca
    14101 attcccccgc aaactcgtat tccgccagtt tttcgggtgc aacctgcccc gatgcggcaa
    14161 ggatgccaat tgcaatcggc aaatcgaaac gccccgactc tttgggcagg tcggcggggg
    14221 cgaggttgac ggtaattttt ttggcgggga attcaaaacc gctttgaata atggcggcac
    14281 ggacgcggtc gcgactttcc tttacttccg tatcgggcag cccgacgatg ttgaaatgcg
    14341 gcaggccgtt ggcaaggtgg gcttccactt cgaccaacgg cgcattcata ccgctcaagg
    14401 cgcggctgta aaccaaggca agcgacatat ttcagacggc cttattcgcc ggcttcggtt
    14461 tgctgcctga tttcggcgac ggcttcttcg gcagcggctt cagccgcttc caatgctgcc
    14521 cgttcgggat tttgcgcggc ttcgagtttt gccaaacgcg cttccaaagc cgccagtttg
    14581 gtacgagttt tgattaaaac ctgctgctgg atgtcgaatt cttcgcgcgt aaccagatcc
    14641 atacggttga acgcgccgcc cagcatcgcc ttaatatttt tttccacatc tttggcaggg
    14701 ctgttggcga tggtttcgct gattttcgaa ccgacttctt caaaaagctg cttgccgaac
    14761 ataatctgta tccttcctga acatatcaaa ttcaatcggc tattgtataa ggaaaaatgc
    14821 cgtctgaaaa cgggcggcgg ataatcggca aaacataccg cgcctccttt gcggttgcta
    14881 aattacacct caaaacacct gccgcctgaa aacggatttc atattccgcg caacgccaga
    14941 ttacaagaca ttacaaaaca atatactgtt ttaaatgatt tttccgacgc gcatcgtttc
    15001 aaactcggct ttttaagcca ttaagtgctt tgcaaacaac agaaattggg ttatgctgaa
    15061 acggattatt tacaatttca tatagtttta ttacatatct tattgtgatt gaagataatt
    15121 tatccgaatc cccctttcgg gtatccggat tttccgttgt acttttatta gaaaaacatt
    15181 tcaggcgcaa gttgcttgca atttcaaagc ctgtaattct gaagttttag acgaggaata
    15241 gcacatgaat cccatgtaca ttacttttgc aatctatctg gttgcagtcc ttctcatcgg
    15301 gcttgccgcc tatttttcta cgcgcaattt cgacgattat attttgggcg ggcgcagcct
    15361 aggcccgttt gttaccgcga tgtcggcagg cgcgtccgat atgtccggct ggctgctgat
    15421 gggtctgccg ggcgcgattt atttgagcgg tttgaatgag gcttggattg ccatcggcct
    15481 cttggtcggc gcgtatttca actggctctt ggtggcgggc cgcctgcgcg tgcataccga
    15541 atacgccaac aatgcgctga cgctgccgga ttatttcttc caccgcttcg gcgcgggcgg
    15601 acacttgatg aaagtggttt ccgcactgat tatcctgttc ttcttcacga tttattgcgc
    15661 ctcgggcatt gtggcgggcg caaccctgtt ccaaagcctg tttgaaggta tgacttacaa
    15721 tcaggcaatg tggctgggcg cgggcgcgac catcgcctat accttcttgg gcggcttctt
    15781 ggcggtcagc tggacggata cgttgcaggc ttctttgatg attttcgcgc tgattttaac
    15841 gccggtaatg gtctatctgg gcttgggcgg cgcggaacag atgtctgccg cgattcaaag
    15901 cgtcgccgca ggcacgggca aagaatacgg cagcctgttt gccggtacga ccgtcatcgg
    15961 catcatttcc accgccgcat ggggcttggg ctatttcgga cagccgcaca ttttggcgcg
    16021 ctttatggcg gccgaaagcg cgaaatcgct ggtatccgca cgccgcatcg gcatgacttg
    16081 gatggcgttg tgccttgcgg gcgcggtggc ggtcggttat ttcggcattg cgtatttcgg
    16141 tgcaaatccc gacaaagttt cttctatgag cggcaaccac gaacgcatct tcatcgcgct
    16201 ttccaccctg ctgttcaacc cttggatcgc cggcatcatt ttgagcgcga ttttggcggc
    16261 ggtgatgtcc accctgtcct gccagctttt ggtttgctcc agcgcgatta ccgaagactt
    16321 ctacaaaggc tttttgcgta aaaacgcgca acagtcggaa ctggtatggg tcggccgcct
    16381 gatggtcttg gcgattgccg tgatttccat cctgattgct tccgatccga acagcaaagt
    16441 attgggactg gtgtcttatg cgtgggcagg tttcggtgcg gcgttcggcc cgattgtgat
    16501 tctgtccgta ttgtggaaac gcatcactgc ctacggcgcg ttgtcgggta tggttgcagg
    16561 tgccttaacc gtagtcgtat gggcggaatg gataaaaaaa ccggctcaag cagcaggcga
    16621 aagcggcttg ttgacgatgt acgaaatcgt cccgggcttt attgtttgct taatcattgc
    16681 cgtgttggtt tccctgttca acaaagaacc ttcgcgcgaa atccaaaaac gctttgaaaa
    16741 agccgacgca gattaccgcg ccgcccgata aacccctttc agacggcacg gcatcccggt
    16801 tcgtgccgtc tgaacccgtt tccgggtcaa acacaaataa accttgcctg catacaggcc
    16861 ttatttgtgt tttacccgtt tcattttcaa agaaacggaa gcacctaccc tgcccgcatt
    16921 tgaagccttg tatttcatct gcggatttat tacgatatta ccgtattcag gccgcaccga
    16981 tgccgcctgc cccccgaaaa actttggaga atccaaaaaa tgtttcattt tgcatttccg
    17041 gcacaaactg ccctgcgcca agcgataacc gatgcctacc gccgtaatga aatcgaagcc
    17101 gtacaggata tgttgcaacg tgcacagatg agcgacgaag agcgcaacgc cgcctccgag
    17161 cttgcccgcc gtttggttac ccaagtccgc gccggccgca ccaaagccgg cggcgtagat
    17221 gcgctgatgc acgagttttc actctccagc gaagaaggca tcgcgctgat gtgtctggca
    17281 gaagccctgc tgcgtatccc cgacaacgcc acgcgcgacc gcctgattgc cgacaagatt
    17341 tcagacggca actggaaaag ccatttgaac aacagccctt ccctcttcgt caacgccgcc
    17401 gcctggggcc tgctgattac cggcaaactg accgccacaa acgacagaca aatgagttcc
    17461 gcactcagcc gcctgatcag caaaggcggc gcaccgctca tccgccaagg cgtaaattac
    17521 gccatgcggc ttctgggcaa acagttcgta accggacaga ccattgaaga agccctgcaa
    17581 aacggcaaag aacgcgaaaa aatgggctac cgcttctcct tcgatatgtt gggcgaagcc
    17641 gcctacaccc aagccgatgc cgaccgctac taccgcgact atgtcgaagc catccacgcc
    17701 atcggcaaag atgcggcagg acaaggcgtt tacgaaggca acggcatttc cgtcaaactt
    17761 tccgccatcc atccgcgcta ctcgcgcgcc caacacggcc gtgtgatggg cgaactgttg
    17821 ccgcgcctga aagagctgtt ccttttgggt aaaaaatacg atatcggtat caacatcgat
    17881 gccgaagaag ccaaccgtct ggagctgtct ttggatttga tggaggcttt ggtttcagac
    17941 cctgacttgg ctggctacaa aggtatcggt ttcgttgtcc aagcctacca aaaacgttgt
    18001 ccgttcgtta tcgactacct gattgaccta gcccgccgca acaaccaaaa actgatgatc
    18061 cgcctcgtca aaggcgcgta ttgggacagc gaaatcaaat gggcgcaagt ggacggcttg
    18121 aacggctatc cgacctacac ccgcaaagtc cacaccgaca tctcctacct cgcctgcgcg
    18181 cgcaaactgc tttccgcgca agacgcggta ttcccgcaat ttgccacgca caacgcctac
    18241 actttgggcg cgatttacca aatgggcaaa ggcaaagact ttgaacacca atgcctgcac
    18301 ggcatgggcg aaaccctgta cgatcaagtc gtcggcccgc aaaacttagg ccgccgcgtg
    18361 cgcgtgtacg ccccggtcgg cacacacgaa accctgctcg cctacttggt gcgccgcctg
    18421 ttggaaaacg gcgcgaactc gtctttcgtg aatcaaatcg ttgatgaaaa catcagcatc
    18481 gaccgcctga ttaagagccc gttcgacacc atcgccgaac aaggcatcca cctgcacaac
    18541 gccctgccgc tgccgcgcga tttgtacggc aaatgccgtc tgaactcgca aggcgtggac
    18601 ttgagcaacg aaaacgtatt gcagcagctt caagaacaga tgaacaaagc cgccgcgcaa
    18661 gacttccatg ccgcatccat cgtcaacggc aaagcccgcg atgtcggcga agcgcaaccg
    18721 attaaaaacc ctgccgacca cgacgacatc gtcggcacag tcagctttgc cgatgccgcg
    18781 cttgcccaag aagcggttgg tgcagccgtt gccgcgttcc ccgaatggag tgcgacacct
    18841 gccgccgaac gcgccgcctg cctgcgccgt ttcgccgacc tgctcgaaca gcacacccca
    18901 gcactgatga tgcttgccgt gcgcgaagca ggcaaaacgc tgaacaacgc cattgccgaa
    18961 gtgcgcgaag ccgtcgattt ctgccgctac tacgcaaacg aagccgaaca taccctgcct
    19021 caagacgcaa aagccgtcgg cgcgattgtc gccatcagcc cgtggaactt cccgctcgcc
    19081 atctttaccg gcgaagtcgt ttccgcattg gcggcaggca acaccgtcat cgccaaaccc
    19141 gccgaacaaa ccagcctgat tgccggttat gccgtttccc tcatgcacga agccggcatc
    19201 ccgacttccg ccctgcaact cgtcctcggc gcaggcgacg tgggtgcggc attgaccaac
    19261 gatgcccgca tcggcggcgt gatttttacc ggctcgaccg aagtggcgcg cctgatcaac
    19321 aaagcccttg ccaaacgcgg cgacaatccc gtcctgattg ccgaaaccgg cggacaaaac
    19381 gccatgattg tcgattccac cgcacttgcc gagcaagtct gcgccgacgt attgaactcc
    19441 gccttcgaca gcgcgggaca acgctgctcc gccctgcgca ttttgtgtgt ccaagaagac
    19501 gttgccgacc gtatgctcga catgatcaaa ggcgcgatgg acgaactcgt cgtcggcaaa
    19561 ccgattcagc tcactaccga tgtcggcccc gtcatcgatg ccgaagcaca gcaaaacctg
    19621 ttgaaccaca tcaacaaaat gaaaggtgtt gccaagtcct accacgaagt caaaaccgcc
    19681 gccgatgtcg attccaaaaa atccacgttc gttcgcccca tcctgtttga attgaacaac
    19741 ctcaacgaac tgcaacgcga agtcttcggt cccgtcctgc acgtcgtccg ctaccgcgcc
    19801 gacgaactcg acaacgtcat cgaccaaatc aacagcaaag gctacgccct gacccacggc
    19861 gtacacagcc gcatcgaagg cacggtacgc cacatccgca gccgcatcga agccggcaac
    19921 gtttacgtca accgcaacat cgtcggcgca gtcgtcggcg tacagccctt cggcggacac
    19981 ggtctgtccg gcacaggccc caaagcaggc ggttcgttct acctgcaaaa actgacccgc
    20041 gccggcgaat gggttgcccc gaccctgagc caaatcggac aggcggacga agccgcactc
    20101 aaacgcctcg aagcactggt tcacaaacta ccgttcaacg ccgaagagaa aaaagccgca
    20161 gcggccgctt tgggacacgc ccgcatccgc accctgcgcc gtgccgaaac cgtccttacc
    20221 ggaccgaccg gcgagcgcaa cagcatctca tggcacgcgc ccaaacgcgt ttggatacac
    20281 ggcggcagca cggttcaagc ctttgccgca ctgaccgaac ttgccgcctc cggcatacag
    20341 gcagtggtcg aacccgacag ccccttggct tcctacaccg ccgacttgga aggtctgctg
    20401 ctggtcaacg gcaaacccga aaccgccggc atcagccacg ttgccgccct gtcgcctttg
    20461 gacagcgcac gcaaacagga acttgccgcc cacgacggcg cactcatccg catcctccct
    20521 tcggaaaacg gactcgacat cctgcaagtg tttgaagaaa tctcttgcag cgtcaacacc
    20581 acagccgccg gcggcaacgc cagcctgatg gcggtcgccg actgatttta ccgaaatccc
    20641 cgggcgcggg ccgtgaacca atgccgtctg aaaacctttc agacggca
//
LOCUS       DAXHMX010000019        20558 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHMX010000019 DAXHMX010000000
VERSION     DAXHMX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20558
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.019"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            99..740
                     /locus_tag="ACFM4S_000523"
     CDS             99..740
                     /locus_tag="ACFM4S_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6050261.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            1077..1583
                     /locus_tag="ACFM4S_000524"
     CDS             1077..1583
                     /locus_tag="ACFM4S_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050262.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFHYIVCNLSAISSFCLM
                     KVIHSYTILCLLIMIIGIAFGFILRNRVLSIVKNKQNFLSDMFPLEMPFFSRVYKIPI
                     LARFLNRGMKWLYLQNFFCLVMICFSISLILNEERGWMPYFACYWFGLCYPVSTYYVF
                     LSRDYKHI"
     gene            1719..2519
                     /locus_tag="ACFM4S_000525"
     CDS             1719..2519
                     /locus_tag="ACFM4S_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6050263.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(2971..3609)
                     /locus_tag="ACFM4S_000526"
     CDS             complement(2971..3609)
                     /locus_tag="ACFM4S_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6050264.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            3864..4706
                     /locus_tag="ACFM4S_000527"
     CDS             3864..4706
                     /locus_tag="ACFM4S_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6050265.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVERPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            4772..5434
                     /gene="exbB"
                     /locus_tag="ACFM4S_000528"
     CDS             4772..5434
                     /gene="exbB"
                     /locus_tag="ACFM4S_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6050266.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            5455..5889
                     /locus_tag="ACFM4S_000529"
     CDS             5455..5889
                     /locus_tag="ACFM4S_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6050267.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(6380..6808)
                     /locus_tag="ACFM4S_000530"
     CDS             complement(6380..6808)
                     /locus_tag="ACFM4S_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6050268.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(6873..8621)
                     /locus_tag="ACFM4S_000531"
     CDS             complement(6873..8621)
                     /locus_tag="ACFM4S_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6050269.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPFGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTIMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTENLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            8948..10381
                     /gene="ccoN"
                     /locus_tag="ACFM4S_000532"
     CDS             8948..10381
                     /gene="ccoN"
                     /locus_tag="ACFM4S_000532"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6050270.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            10408..11019
                     /gene="ccoO"
                     /locus_tag="ACFM4S_000533"
     CDS             10408..11019
                     /gene="ccoO"
                     /locus_tag="ACFM4S_000533"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6050271.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            11024..11194
                     /locus_tag="ACFM4S_000534"
     CDS             11024..11194
                     /locus_tag="ACFM4S_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6050272.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            11218..12096
                     /gene="ccoP"
                     /locus_tag="ACFM4S_000535"
                     /pseudo
     CDS             11218..12096
                     /gene="ccoP"
                     /locus_tag="ACFM4S_000535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            12205..12553
                     /locus_tag="ACFM4S_000536"
                     /pseudo
     CDS             12205..12553
                     /locus_tag="ACFM4S_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            12755..14164
                     /locus_tag="ACFM4S_000537"
     CDS             12755..14164
                     /locus_tag="ACFM4S_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6050273.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            14266..17475
                     /gene="recC"
                     /locus_tag="ACFM4S_000538"
     CDS             14266..17475
                     /gene="recC"
                     /locus_tag="ACFM4S_000538"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6050274.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGSRDTLLHALQTDIQNLDMPSEKAGNINTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYTLECLSDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWQQPATVGEWVARCRDLLETLFQTEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGQDFSQTAARIRAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAEMRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTFAAAEKKEEAGAA"
     gene            complement(17545..19113)
                     /gene="mtrF"
                     /locus_tag="ACFM4S_000539"
     CDS             complement(17545..19113)
                     /gene="mtrF"
                     /locus_tag="ACFM4S_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC6050275.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            19445..19657
                     /locus_tag="ACFM4S_000540"
     CDS             19445..19657
                     /locus_tag="ACFM4S_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050276.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYENIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
     gene            complement(19750..20373)
                     /gene="mtrR"
                     /locus_tag="ACFM4S_000541"
                     /pseudo
     CDS             complement(19750..20373)
                     /gene="mtrR"
                     /locus_tag="ACFM4S_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
BASE COUNT         5280 a         4708 c         5358 g         5212 t
ORIGIN      
        1 ttatttattt acggtgcata ggcgggaaga taaacttgtt tcgggtggcg cggggtgtta
       61 aggtagccgg taccgacagg ttgacgagga atattttgat gaaactgtat atttacgacc
      121 attgcccgtt ttgcgtgcgt gcgcggatgg ctgccgggct gtttggcgcg gacgttgaag
      181 aagccgtgct ggcaaacgac gacgaggcaa cgccgatcgg tatgatcggt gcgaaacaag
      241 tgcccgtcct gcaaaaagaa gacggttcgt ttatgggcga gagtttggac attgtgcggc
      301 atttcgaccg cgaagaccgt ctgaaagacg aagtccgccc cgagatacgg gcgtggctgg
      361 acaaggtggg cggatacaac gacaaactgg tgcagccgcg cgtgatcaaa atcgggctgc
      421 ctgaatttgc aacgccggaa gcggtgaaat attttacaga caaaaaagag aaaagcatcg
      481 gcagcttttc cgccaatttg aataaaaccg cccaatacct cgaacggata aacgcagatt
      541 tgcaagagtt ggaaaatttg atggacggtg cttcagacgg catcaacggc ggaatcggta
      601 tggaggatat tttggttttc cccgttttga ggaatctgac cgtggtgcgc ggcatcgcgt
      661 tccctcggaa gacgatggat tatttaatcg gtatgagtga aaaatcgggc gtgccacttt
      721 atttcgacag ggcattgtaa aagtggctgt tattaactga cagatgaagg aggttctacc
      781 ctatttgagg taaactctat ttaccattgg ccgaaatacc agtgcttatc tccaagacga
      841 ccttcccaat cccttttgcc tcttaaaaag aagtgactag tgtgtaaagt tatagttcct
      901 tttatagctt tccaataaaa ttaaagtatt taaaagcttg tttagttcta atttgttaat
      961 ttctgtatct aaataatgtt cagaatagta ttatttttta aataaaccat gaatagtact
     1021 tcacattgtt tgtcgtatta tttagctttt aaatttctaa tgtgaaagat agtttaatgg
     1081 acgcctttga tttttttatg caaggcaatt ttaaaaaaat tgcaactgaa caaggtttag
     1141 accgtaaata ttttatattc cactatatcg tatgtaattt atctgccatt tcttcttttt
     1201 gtttaatgaa ggttatccat agttatacta tcctttgttt attaataatg ataattggca
     1261 ttgcatttgg atttatcttg agaaataggg ttctatctat agtaaaaaat aaacaaaatt
     1321 ttttatctga tatgtttccc ttggaaatgc ctttcttttc tagagtatac aaaataccta
     1381 tcttggccag attcttgaat aggggaatga aatggcttta tctccaaaat tttttctgtt
     1441 tagttatgat ttgtttctcg atatctctta tccttaatga agaaaggggg tggatgcctt
     1501 attttgcctg ctattggttt ggactttgtt atccagtaag cacttattac gtttttttat
     1561 ccagagatta taaacatatc taaactatta tgcgtggcgg tacttccaaa ggtaaatttt
     1621 aacctaatca atttactcaa accatccaaa aattaccaag cagcaatata aacacagatt
     1681 tatttgagcc aacctgatca aaacaaggaa ctaaatagat gaaaaagacc atttttaaca
     1741 ttaccaaaat ggactgcccg tccgaagaac agctcattcg catgcgttta aaagatgtgt
     1801 ctgatattta tgaattacag tttgatattg ctgggcggtg cttaaccgtc tatcacgata
     1861 atcaagatac aacgatttta caagtacttg aaccgcttaa ttttgatagt catatcattt
     1921 ccaccgaagt gattgttgat aagatagtgt ttaacaagcc tgatgagcac ttagaaaaaa
     1981 gattattata tcaagtgctc atgattaact ttgtgttttt tatcatagag tgttcggttg
     2041 gtatttttgc taattctatg gggctgattg ctgatagtct agatatgctg gcggacagct
     2101 ttgtctatat attggcgcta tcagccattg gtatgacgct ggcgtataaa aaacgtgtgg
     2161 catttttggc ggggataacg cagataatat tggcgttatt tggggtgata gaagtgatta
     2221 ggcgttttat cggtgctgag cagctgccca attatcaact gatgattggc acggcatttt
     2281 tggcattgat tgcaaactgg ctgtgcttat atctactgag caaaaatcaa aataaagaaa
     2341 tccatatcaa agccagcatg atttttacat ccaatgacat tatcatcaat atcggtgtga
     2401 tagcggcggg agcattgacg ttattgactc attctagcta tcctgattta atcattggca
     2461 tgattgtctt tgtcattgta ctatttggtg ccagaaatat tttaaaattg gcaaaataat
     2521 tcctttcacc agcaaaggag tttttatgca aatatcggtc aaagctacct acatacgtgg
     2581 cagtacttcc aaaaacttga ttttttaaaa tgtggttaat gcgagtttgt ttatctggta
     2641 tttgattggc caagccttca tccaagcgtt tgacgatttc acgcaaaaat acccctgatt
     2701 tacacacagg gtctaagaat gtcgcctttg gatttgacca aatctcagac ggcaataaat
     2761 ccagcatttg attggcaagt tttggcgatg tgaaaacctc atcattagac agactggagt
     2821 ttcttaatca gtgtttcagt taatgaaagg tattatcatg caacttggtt ctaataatga
     2881 tgaaaaaaat aagattacac tacaggaaaa cgcggcaggg caatatgtcc tgccgcgttt
     2941 tctggagcgt ttatgccgtc tgaaagccag tcagacgaac atctgcacaa acgcaatcac
     3001 cagccgcaca atcggtgcaa taaacgcacc caaaaccccg gtcagcataa gcagcaggat
     3061 aatccacgtc ccataaggtt cgattttgcg gaacgcttgc gaatatttcg ccgacaggaa
     3121 ggtgtcgatg aaaatgccgc cgtcccaagg caggatgggg atgatgttga gcgcgaacag
     3181 aatcgcattg atcagaatac cgtagtttgc catttgagcc aacggcatct gatacgcccc
     3241 accgacatac ggagtcagca ccaaaaccac gccccacaga acagccatcg ccagattcga
     3301 cagcgggccg gacgcggcaa cgcaacgcca ggcaaggcgc gggttgcgga agttgcgcga
     3361 atcgatagga atcggacgcg cccagccgaa caggaagggc gtaaacatca aagtaagcag
     3421 cggtacgatg attgtgccga ccaaatcgat atggggcagg gggttcagtg tcagcctgcc
     3481 gtattgttcg gcagtgttgt ctccccagta gcgcgccgta tagccgcgcg ccacctccct
     3541 gacggtaatg gcgagcagca cgggcaggac ggcaagcaga aacacgccca aatcaaaatt
     3601 ttgaaacatg gttttccttt gaacagatgt ggtgtgcagc ccgaaacggc tgacagacag
     3661 aagcggcaat atatcatcta acctgatttt attggaataa gaaatcagac ggaatcattt
     3721 tttctgccgg cccgttatct gatgatttgc tgtaatacag cgtttaaaca cggtttgcct
     3781 gtgaacggaa cagacattca tatgaccaaa aattgtaaaa ataggttgca aataggaatt
     3841 gttgctatta ttatgcccga attatggata aagaacgaat tttaaccccc gcagtcgtgt
     3901 tttccgtcgc actgctgcat ttggcaatgg tggcattgct ctggcaggcg cacaagctgc
     3961 ccgtgataga gtcaggaaat gttatcgaat ttgtcgattt gggtgatttt ggcggagggg
     4021 acggtgcacc cgaaggcgca ggtgcgcctg ctgcgcctga gccgcaacct gagcctgagc
     4081 cgcccaaacc tgtcgagccg cccaagccgg ttttgaagcc ggtggttacg aaaaaggcgg
     4141 atgcggatat tcagcagcct aaggaagagc cgaaacctga agaaaagccg aaacccgaag
     4201 aaaaaccgaa accagagcct aagccggaag cgaagcctgt cccgaaaccg gcggaaaaac
     4261 cggtcgagag gccgtctgaa aaacctgccg aacatcccag caatgcttct gccaaagcag
     4321 acagcgagca gggtaatggg gaagataagg gaaccggtac caaaggagac ggaacggggc
     4381 gcggagaagg cagcggtaaa ggtagcggcg gtgtcaaagg cgaacacggg gaaggagccg
     4441 gcagcagcaa aggcaatcct ttacgcgcca atggcagcat tccgcgcccg gcttatccca
     4501 cgctttctat ggagaatgac gagcagggta cggttgtttt gagcgttttg gtttctccgg
     4561 gcggtcatgt cgagtccgtt aaaatcgtga aaagcagtgg tttttctcgg ttggacaatg
     4621 cggcacgcaa ggcggcgcaa aacgggcatt ttcaagccaa tgcctggacg gagtttaaag
     4681 tccccgtcaa gtttgaattg aattaggcgg ttgccgcagc ggtttgcggc agcttggatg
     4741 tttgattggt tttttcagtg ttggaatcat tatgaatttg aaattagtat ttgaatcggg
     4801 cgatcccgtc ctgatcggtg tgtttgtgtt gatgctgttg atgagtatcg taacgtggtg
     4861 tttggttgtc ttgcgctgca tcaagctgta tcgggcgcgc aaagggaatg ccgccgtcaa
     4921 acggcatatg cgcgatactt tgtcgctgaa cgacgcggtc gaaaaagtgc gcgccgtcga
     4981 tgcgcctttg tccaaactgg cgcaagaggc attgcagtct taccgcaact accgccgaaa
     5041 cgaagcgtcc gaactggcgc aggctttgcc gttgaacgag tatttggtca ttcaaatccg
     5101 caacagtatg gcgcagatta tgcgccggtt tgattacggg atgaccgcgc ttgcctccat
     5161 cggcgcgacc gcgccgttta tcgggctgtt cggcacggtt tgggggattt accacgccct
     5221 gatcaatatc gggcaaagcg ggcagatgag tattgcggcg gttgccggcc cgattggcga
     5281 ggcactggtg gcgacggcgg cgggtttgtt cgtggcgatt ccggcggtgt tggcatacaa
     5341 cttcctcaat cgcggcacaa aaatactgac ccaggatttg gatgcgatgg cgcacgattt
     5401 gcacgtccgc ctgcttaatc aaaaggatag ctgattataa aaggatagct gattatggca
     5461 tttggttcga tgaattccgg cgacgattct ccgatgtccg acatcaacgt tacgccgttg
     5521 gtggacgtga tgctggtgtt gctgattgtg tttatgatta ctatgccggt gctgacgcat
     5581 tccatccctt tggaactgcc gaccgcgtcc gagcagacaa acaagcagga caaacagcct
     5641 aaagaccccc tgcgcctgac gattgatgcg aacggcggct attatgtcgg cggggattct
     5701 gcaagcaaag tggaaatcgg ggaagtggaa agccgtctga aagccgccaa ggagcagaat
     5761 gaaaacgtga ttgtggcgat tgcggcagac aaggcggtgg aatacgatta tgtaaacaaa
     5821 gctttagaag ccgcccgtca ggcaggaatc accaaaatcg gttttgtaac cgaaaccaag
     5881 gcgcaataaa gttcgacgct tgaacgaatg ccgtctgaaa gtctttcaga cggcatttct
     5941 tatgtttaat cagctatata tagttgatta aaacaaaaat gagacgacag agcgccgtct
     6001 ccatttctgc gcaggcggga atacaaacat ctgatttttc ttttattttc aaacattact
     6061 gaaatgggta agtctggagt cctgcctgcg cgagaataat gaaaatgtgc atttctaatt
     6121 tttatagtgg attaacaaaa atcaggacaa ggcaacgaag ccgtagacag tacaaatagt
     6181 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagttaaggc
     6241 gaggcaacgc cgtactggtt tttgttaatc cgctataccc actgtaaaaa cggagaagga
     6301 cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt tgcggtggaa tgccgcttga
     6361 aggtgttttc ggcttgtaat cagacgcggg cgttgtccag ccgctctctg aaattttcca
     6421 aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc aaccggcata ccttctgccg
     6481 cccggcggaa gccccacagg agggagcagc gcgtaccggt ccggcaatag gcaaggacgg
     6541 gatattcggc ttgtccgatg agttggcgga aggtttcgac atcgtgtttt tggatgtcgc
     6601 gtgcggtaac gggttggtga tggaatccag taacgcctgc ttgttccagc cactgtttga
     6661 tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg gcggttgcag atgacggttt
     6721 tgatgcccag ttgcgcgatt tgttccgcat cggctttggt cagttgcgga gaaatataga
     6781 ggtgttcgtc aagtttcaga atggccattt tgtttccttt actttgcggt tgtttttcag
     6841 acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga acaaccagtt gtccaacagg
     6901 ttccggtctg tgatatgtag gatggcagta tccgctgccg ctgcctgaac cgtcaagacg
     6961 gtttgacgca atatgccggc acggaagaag tggatattga tttttgcatt gacgtggtat
     7021 cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt ctaaagcaat gattttgtct
     7081 tgcggacaca gtgccgcaga ttccgcgctg ccgccgttga agacatgggt caggacgatg
     7141 tggtcggcgt tttgtttgaa acgtgcgcca aaatcgcctg ccgacgggac ggggcagatg
     7201 tgttctgcgt atccgccgcc gtgttgtcgg ggaagcggca ggaaggtcag tcccacgcct
     7261 gcggttgcca ggcattcggc aagcggcaaa ttttcggtac tgtataacgc tttttggaaa
     7321 aaatcttcca aatccaagcc ggtaatttcc tgacagcgga tttgccagtg tttttccgga
     7381 atacccgagt gtgtgtccct ccactcccga tagagtttgt ccattatcgt atcgagagaa
     7441 tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc acaatgcggc aagcgcgcct
     7501 ttctggtagt agctgacgat ggcgttgggg ctgttttcat caggtttgta aaatttgttc
     7561 cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac ggccgcgggt ttgttgtacg
     7621 cgcgtaatgc cttgtgccag caggtttaaa taagattcgg gcgagatggt gcggctgcgt
     7681 gccaaaaaca aatcgtcgta ataggatgta atgccttcga atgcccatag ttgttcggta
     7741 tagttttctt tgtcgaggtc ataaggggcg aacgcggcag gtttgatgga tttgacgttc
     7801 cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg tggtgtaggc atcgtcggca
     7861 tcggtcatac cgaacggcgg aaggctgtgg cggtcggcga gcagggcggt gctgtcggcg
     7921 tgttccaaac cgccgtaaat atggtcgccg acgtggagca ggaacaaata tttttgaaac
     7981 ggggcagggg aggaaaacac cgccagttct gtttcgcaga tttttttgat atccgaaacc
     8041 agcctgttgc ggtcgaaatc gggatagatg ccgcttaagg caattgtgtg cggaatgcct
     8101 gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga caggtcggtc aatcaattcg
     8161 gcataagatg ccgcctgaaa gacaaacctc cctgtttccg gcagcgttgt ggcaatacgc
     8221 cattcggacg gaatacccgt caattccaag cggtgcggca gcgtttccgt tccttcgact
     8281 ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg tcgtcaggaa agaacctcga
     8341 accgacaaat cgaatgcata tacggtgtag cggatttgcc actcgccgcg tacggcggca
     8401 gcgtgccagc ggttttttgc aatttgttcg agcggcatgg acgtgccgtt acaggatgca
     8461 tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat agctgcccgg aacccagttt
     8521 ggcaggctta tttctatagg aagatcatca tcttgtgtga atgtcagcag gatgtgccac
     8581 tcgtggtcga gaggggacgg ggttatttta taatgaatca taactatcaa atatatcgtt
     8641 aaacgggatg gattaaatac ctttaaaatg cagcttaacc tacatcaata aagtgccaca
     8701 aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt gtaatttatt ataacaatat
     8761 gatttatatg taataaaaaa cagattaagc aaagggctcg ccggcggcgc gaatgtaatt
     8821 ccgcttggcg taataaggca gatatgttaa atttagaaaa aattacgctt gcccggattg
     8881 caccttaaac aggctgcgga agcggcagga taaaaatctt tattcccata aattcatgga
     8941 gactctcatg gacacacaaa cttacaacta caaagtggtg cgccagttcg ccatcatgac
     9001 tgtagtttgg gggattgtgg gtatgttggt cggcgttatc gtcgccgccc agctttttgc
     9061 tcctgccctt gacttgtcta atatcggacc ttggttccac ttcggccgcc tgcgtccgct
     9121 gcacaccaat gcggttattt tcgcatttgg cggttgcggc ctgatcggca catcatacta
     9181 cgttgtccaa cgtacttgta atacccgtct tttcggcggt tggctgccgg catttacctt
     9241 ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc ttccctatgg gttggaccca
     9301 aggtaaggaa tatgccgaac tggaatggcc gatcgatatt ctgattactt tggtttgggt
     9361 ggcttacgcc atcgtattct tcggtacgat tgccaaacgt aagattaaac atatttacgt
     9421 tgccaactgg ttctacggcg gctttatttt ggcggtcgca cttttacaca tcgtcaacaa
     9481 tatcagcatc ccggcagggt tgatgaagtc ataccccgtc tattccggtg cgattgatgc
     9541 tatggttcaa tggtggtacg ggcataacgc cgtgggcttc ttcctgactg ccggcttctt
     9601 gggtatgatg tactatttcg tacccaaaca agcagcccgc cccgtttact cctaccgcct
     9661 gtccgtcgtt cacttctggg cgttgatttt tacctatatg tgggcgggtc cgcaccatct
     9721 tcactacact gcgctgcctg actggacgca atctttgggt atggttctgt ctttgattct
     9781 gttcgcaccc tcttggggcg gtatgattaa cggcatcatg accttgtccg gcgcgtggga
     9841 caaactgcgt acagacccga ttcttaaatt cctgattgta tccttgtcct tctacggtat
     9901 gtctaccttt gaaggcccga tgatgtcgat taaaacggtc aatgcattga gccactatac
     9961 ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc tgggtaggct ttgtaaccat
    10021 cggttccgtc tattacatga ttccccgtct gttcggcaaa gaacagatgc acagcaccaa
    10081 gctggtagaa gcacattttt ggattgcgac catcggcgtg gttctgtata tcgctgccat
    10141 gtggattgcc ggtgtgatgc agggtttgat gtggagttct ttgaacgatg acggtacgct
    10201 gacctactcg tttgtcgaat ccgtaaaacg caccatgcct tactacgtga tccgttttgc
    10261 aggtggttta ttgtatctga gtggtatgtg cattatggcg tacaacgtgt accgcacagc
    10321 catcggtggt aaagcagtcg atgccgaaat ccctgcggtt tcccaaacac agcaccacta
    10381 aaatactaag aaagataggc taccaaaatg aaattacaac aattggctga agaaaaaatc
    10441 ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg gtctgttgat tgaagttgtg
    10501 cccttggcct ttaccaaggc ggcaacacag ccggcgccgg gcgtgaagcc ttacaatgcc
    10561 ttgcaggttg ccggacgcga tatttacatc cgtgagggct gttacaactg ccactctcaa
    10621 atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc attactctgt tgccggagag
    10681 tcggtttacg accatccgtt ccaatggggt tccaaacgta ccggtcctga tttggcacgt
    10741 gtgggcggtc gctattccga cgaatggcac cgtatccacc tgctgaatcc ccgtgatgtc
    10801 gtgcctgagt ccaatatgcc ggcattcccg tggcttgcac gcaataaagt cgatgtcgat
    10861 gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta ctccttacag tgatgaggaa
    10921 attgcgaaag cacctgaggc tttggcaaac aaatccgagc tggatgctgt agtcgcctat
    10981 ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac atcatggata ttaacggtat
    11041 tcgtgctctc ttcacggtat ggatctttat ctgtttcctg ttggtactct atatcgtctt
    11101 caacaggcgg aataagaaaa actacgataa tgccgcaaac agcatttttg atgaaaacca
    11161 agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt gataacggag caaaacaatg
    11221 aacacaacat cccaatttac tagtaatttc tggaatatat atattgcagt tattgtctta
    11281 ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa atgttgtcaa acgtccgaag
    11341 aagggcgaag aagtacaaac tacgggtcat gagtgggacg gcattgccga atacgacaat
    11401 ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt ggctgttcgg tatcggttat
    11461 ttggttatgt atccgggtgt cggcgactac aaaggtctgc tgaaatggac cagccataac
    11521 caatatgaaa aagaggtcaa aaaagccgat gagcaatacg gcaaactgta tgccaagttt
    11581 gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag ccaagcaaat cgcccaaaac
    11641 ctgtttaaca cttattgtat ccagtgccac ggctctgatg ctaaaggctc taaaggtttt
    11701 ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc ccgataaaat tcacgagacc
    11761 atcgaaaaag gccgtgttgc gactatgcct gcctggggcc ctgctttggg cgaagaaggc
    11821 gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac ccgaaggtca gtatgatgag
    11881 gaacgtgccg cgcgcggaca agccttgttc agcggtccgc ctgccaactg tttcacttgc
    11941 cacggcgata agggacaagg tatccaaggt ttgggtccga acctgactga tgacgtgtgg
    12001 ttgtggggcg gtacgcaaaa atccattatc gaaaccatta ccaacggtcg cagcagccaa
    12061 atgcccgctt ggggacattt cttggataaa gacaaactgc atattatgac tgcttatgta
    12121 tggggtcttt ccgataaaga cggtaaagct ctggtgaaaa aagccgagcc tgcaccgact
    12181 cccgcaccgg cggcagaacc cgcagcctct gctcctgcag aagcagcaca agccgcgtcc
    12241 gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa aagccgcacc tgctgccaaa
    12301 gcggacggca aataggttta tgaaaccgtt tgtgccgcct gccatggcaa tgcggttccg
    12361 ggtattcccc atgtaggcat caaagccgat tgggccgacc gcatcaaaaa aggcaaggac
    12421 acgttcgttg cacaaacacg cgatcgaagg tttcaatacg atgcccgcaa aaggcggtcg
    12481 cggcgatttg agcgatgatg aagtgaaagc tgcggttgac tatatggtca accagtcagg
    12541 cggcaaattc taacttgact gagtttccga tattatcggt tatgaaaatc cagattcagt
    12601 attgaatctg gattttttgt tttaacgggc atccggtttt aaacgcagac tgcctatttg
    12661 ttgtaatgaa ttttgacttt agtcataaag aattagtctt tattgtttat aatcgcgcat
    12721 catcacgata gccctatttt caaaaggacg attaatggat acacaaatca aaactgaagc
    12781 cgacaatcag agcaaccggc gttatctgac cgtttggcgg tggcattttt atgccggtct
    12841 gctggttatg ccttttctta ccctgcttgc cgttacgggt ttgggtatgc tgctgtttgc
    12901 caatattacc ggtaaggagg gcgagcggat tcatgttgtg ccgcaggcaa cggtacaacc
    12961 tctgtctgtt caggcggaag cggcacgcag tgccgttaat ccggaaactt cgtccgtcgt
    13021 tcagtatatt gcgccgcgtg ccgatgatat ggttgccgtg ttccgtgtca acaatgaggg
    13081 caaggcaacg atggtcgcgg tcgatcctta tacggcaaaa gtggtcagta ccatgccgcg
    13141 caatcagggt tggtattacg cgatggatga aatccacagc gatatgatgc tcggttcgac
    13201 aggtgattat cttttggaaa cggctgcatc gctgacgatt atcatgataa tcagcggttt
    13261 gtacctttgg tgggtgaaac ggcgcggcat caaggcgatg ctgctgccgc caaaaggcag
    13321 ggcgcgttct tggtggcgga atctgcacgg cgcgtttgga acttgggtgt cgttgatttt
    13381 actgttgttc tgcctgtcgg gtattgcttg ggcaggtatt tggggcggca agttcgtgca
    13441 ggcttggagt cagttcccgg ccggcaaatg gggtgtcgaa ccgaaccctg tttcagtcgt
    13501 gccgacccac ggcgaggtat tgaatgacgg caaggttaag gaagtgccgt gggttttgga
    13561 gcttacgcct atgcctgttt cagggacgac tgtgggcaaa gacggtatta accctgacga
    13621 gccgatgaca ttggaaaccg tcgaccgttt tgcgcgggaa atcggtttca aagggcgtta
    13681 tcagttgaat ttgcccaaag gcgaggacgg agtgtggact ttgtcgcagg attctatgag
    13741 ttacgacatg atcagcccgt ttgccgaccg cacggtgcat atcgaccagt acagcggcaa
    13801 aatccttgcc gacatccgct ttgacgatta caacccgttc ggcaaattta tggcggcaag
    13861 cattgcgctg catatgggta ctttgggctg gtggagcgtg ttggcgaacg ttttgttctg
    13921 ccttgccgtc atttttatcg gtatcagcgg ctgcgtgatg tggtggaaac gccgtccgac
    13981 cggagcggtg ggcatcgttc cgccggcgca gaaagtcaag ctgccggttt ggtggatgat
    14041 ggcattgccg ctattggcaa tcgcactgct cttcccgacc gcactgcttg ccattgccgt
    14101 gatttggctg ttggatacct tgctgctgtc gcggattcct gttttgagga gatggtttaa
    14161 atgacccgat gccgtctgaa gcgttcagac ggcattttgt ttgaaggcgg gcgggggaaa
    14221 ggctatataa tcccgaatac ttgaccacag cttctgtttg aaatcatgtt ttatctgtat
    14281 caatccaacc gtcttgaaac gctggcggca ctgtttgccc gcattcaaaa agtcaaaccg
    14341 ctgaaatcgg ttttacagcc cgaacagatt attgtgcaga gtcaggggat gcgccgctac
    14401 ctcaatacct gcctcgcccg cgatttgggc gtggcggcga atttgtcgtt cagcctgccc
    14461 gccggtctga cgtggaagct gatgaaaaaa ctgattcccg gtattccgga actcagcccg
    14521 tttgcgcctg aagtgatgcg ctggcggctg ttggatttgt tccgcagcga ggcatttcgg
    14581 aatacggcag aatttgaaga tgtgaggaat gtgctgcaag actatctggg cagcggcgaa
    14641 tcggcggatt atcagcttgc tggccagctt gcagacatat ttgaccaata cctcgtgtat
    14701 cgtccgcagt ggatagacgc gtggcagcag ggcaggatac tcagtttggg cgacgatgaa
    14761 atctggcagt ccaaactgtg gcgttatctt gatgacggca ggcagagcgc gccgcaccgt
    14821 gttgcgttgt gggaaaagct gttggaatct ttgagcagtg ataagctgcc cgagcgttat
    14881 ttcgtgttcg gcatttccac gatggcgccg atgtatttgc agcttttgca caagctgtcc
    14941 gaacattgcg atgtgttcgt gtttgcactc aatccgagtg ggatgtattg gggcaacgtc
    15001 atcgaagcgg cgcaaatcct caaaggcggc ggcgatcccg atttaactca ggcagggcat
    15061 ccgctgctcg cctcattggg caagcagggg cgcgactttt tcgacttttt gaacgaaatg
    15121 gaaatagaag gagaaacgcc ggtgtttgaa gaaggcagcc gcgatacgct tttgcacgcc
    15181 ctgcaaaccg atatccaaaa cttggatatg ccgtctgaaa aagcgggaaa tattaatacg
    15241 gacgacggat cgatacgcat cgtctccgca cacagccctt tgcgcgaatt gcagatactc
    15301 aaagacaaac tgttgaaaat tctgcatgaa catcccgatt ggcagccgca tgatatagcc
    15361 gtattaaccc cgaacatcga atcctatacg ccttttatcg aagccgtgtt cggacaggcg
    15421 cagcccggtg cgcaggcatt gccgtattcc gtttcagacg tgaaaatcag ccgccgacaa
    15481 ccgttttttt atacattgga atgcctgtcc gacttgttgg aaagccgatt tgaagtcgat
    15541 aaagtgcttg tgcttttaga aaccgcaccc gtgttgcgcc gtttcggact gactgaggac
    15601 gatttgccgc ttttgcacga catggttgcc gatttgaacg tccgctgggg tttggacgga
    15661 gaaatgcgcg gcggcacgga tcagctgttt acctggaagc aggcggtaga acgcatgata
    15721 ttgggctgga tgctgcccga aggcggcaat ccgatgtggc aggatgtcag cgcgtggtat
    15781 gccgacgtga accaaaccgc cctgttcgga cgttttgccg ccctcctcga aaccctgtcg
    15841 gatattgtac ggatatggca gcaacctgcg acggtcggcg aatgggtggc gcgttgccgg
    15901 gatttgcttg aaacattgtt ccaaaccgaa gccgatgacc aaaaggcagt ccaaaacctc
    15961 gaaaacgaat gggtcaaatg gcaggcggaa accgaattgg cgcaattttc cggacagttg
    16021 ccgccgcaca ccgtcatccg ccacatccgc cgctttctcg acagcgaaag cgaggcaggc
    16081 tttttacgcg gcggcatcac cttttgcagt atggtgccga tgcggagcct gccctttaaa
    16141 gtcatctgtc tgttgggttt gaacgacgga gattttcccc gcaataccaa agccgccgta
    16201 ttcgacctga ttgccaaaca tcccgccaaa ggcgaccgcg cccgccgcga tgacgaccgc
    16261 tacctgttcc tcgaagccct catcagcgcg cgtgaaatcc tctacctgtc ctacatcggg
    16321 cgcgacatcc gcaaagacga agagcttgcc ccgtcttccc tgttgggcga actcatcgat
    16381 accgttgccg ctatggcggg catcggtagt cgccaacttg cacaaaactg gatagaacag
    16441 catccgctgc aagccttttc acgccgatat ttccaagaag gcggacgttc agacggcata
    16501 ttcggcacgc gtaccgacta cgccaccgcg ctcggacaaa cgcccgaacc gccgcaaccc
    16561 tttttcgacc aacccgtaga aaacgccgaa cctgttgccg aaatcggaca ggacgaattt
    16621 atccgtttct ggcgcaaccc cgtcaaagta tggcttcagc agcagcttgc gtggagcgaa
    16681 ccccatatcg gcgaagcctg ggagcctgcc gaacccttcg agccgcaaca cgccgatcaa
    16741 atcgccgaaa tctatattga ggcgcgccgc gaaggacaag atttttccca aaccgccgcc
    16801 cgcatcaggg cggaaagcct gctgccgtcg ggagagttgg gcagactttg gcagcaggac
    16861 ttccaaaccg ctgccaaaca aatcgacacg gcggttttaa acagtcccaa acttcctccg
    16921 ctgccctacg ccataccgtc ggacgggcaa atcctgaaag gcagcttggg caatctgtac
    16981 cgctgcggac aagtgtttta cgcctacggc aaacccaacg cgccgcaacg tatcgctttt
    17041 ttgttggaac acctgatatt ttgcgccgtt atgccgtctg aagccgaaat gcggcaaacc
    17101 tttatcgtcc aatccggaga aaccgaaata ttggcggaaa tcgcgcaaga cagggcattg
    17161 cagctattgt cggaatggat ggcgtttttc aatatcgggc aaaaccgccc gctgccgttt
    17221 tttgccaaaa cctcgcttgc cgccgccgaa gcgtttgccc aaaaacaaga ttgggaagcc
    17281 gccctgaaaa aagcccaaac cgcctattac ggcagcaaag tcagcaaagg gcagaaagac
    17341 tataccgaag tcgccctcgt gttcggcaac gcaagccaaa acccgctcga acagcccctg
    17401 tttgaaaacc tcgtccgtct gcttgccgac acgtttgccg cagcggaaaa aaaggaagag
    17461 gccggagcag cctgacggga tggaaaggca aatgccgtct gaaaacagca aaaaggtttt
    17521 cagacggcat tttgtttatc gtgtttaagg tgcgggatag aatgtgggcg cgccgggacc
    17581 gacgggcagg cccaaaacaa atacccaaat gcagaataag gcaatccacg caatcaagaa
    17641 gaaagcggaa tacggcaaca tcatagaaat cagcgtaccc acgcccgcat cttttttgta
    17701 tttgatcacc gtcgccataa tcagcccgaa ataactcatc atcggcgtaa taatattggt
    17761 aacggaatca ccgatgcggt aagcggcttg aatgacttcg ggcgcgtagc cggccaacat
    17821 cagcataggg acgaaaatcg gcgcagttac cgcccattgc gcggaggcgg agcctatcat
    17881 cagattgata aaagcacaaa ttaaaataaa accgataaac aacacgctgc cgcccaagcc
    17941 gacttctttt aagaacgtcg cccctttaac ggcaatatat tgcccaatat tcgtccaatt
    18001 aaaaaatgcg acaaactgtg cggcaaaaaa gatgatgacc aaataaagcc ccagagtact
    18061 catcgattcg gccatcgcat taacgacttc ctgttcgccg cgcaaacttc gggttaccct
    18121 gccataaaca atgcccggca gcgcaaacaa caagaaaata aaaacaacaa tcgattttaa
    18181 aaacggcgaa ccggaaacca atcctgtttc aggatgacgc aaaataccgt cggcagggac
    18241 gatgctccaa gccaataggg cggataaggc aacaaacacc acgccagccc aaattaatcc
    18301 tttatattcc aaaggcgtga tttcattgga atgccgaatg tctttttctt cttgtgacaa
    18361 atctgattga taagggccca attgcggttc gacgattttt tcagtaacaa aataaccaat
    18421 caaagcaatc acaaacgtac tggctaccat aaaaaaccag ttggcttcag ggcctacgac
    18481 gtagtcggga tggatgattt gcgccgcctg ttgggtgatg cctgccaaga gcggatcgat
    18541 tgtgcctaag aacagattgg ccgaataacc gcccgaaacg ccggcgaaag ccgcagccag
    18601 accggcaagc ggatggcggc cgagggaatg aaagatgatg gcggacaaag ggattaggac
    18661 gacatagccc aattcagaag cggtattaga taaaatccct gtaaaaacaa ccataaaagt
    18721 agtgagtttg cgtggagatt ttgtgagcaa taagcgcatt aatgcggaaa tcaagcccga
    18781 tttttccgca atccccacgc ccaataaaga aaccaacacc gttcccaacg gcgcgaaacc
    18841 ggtgaaattt ttaacggtat gcgtcaggat tttgatcaaa ccgtcagcat cgagcaggct
    18901 gacaacgtga atcaaaccgt catcggcacg tcctttcgca ccaacagggc gcggatcggg
    18961 gacggatagt ccgaaatacg caccggcggc agaggcaatc agcaataaca caatgaaaat
    19021 aataaaaagc gtaaccgggt gcggcaacat attgcccagc cattcgactg tgcgtaaaaa
    19081 tcgtccgtcc cgttgcgtat cggtttgact catctgcttc tccttaaaaa atatattgag
    19141 tgcattaaat ttttgaatgt aacatgaagt gttttgtaag caaactaaaa taatcgcata
    19201 tgtatataac cgcaatagat taaaacagaa aatgcccaca tcgttattct cataaaggcg
    19261 gtaatccgaa aacttgacgt taaagcctta tcaggaatga ctggaactca acaaagcggt
    19321 ttcccgcttt cgcggaaatg gcgggatttt cggtttttgt ttgcaaaaat gccggagaaa
    19381 aatgcagttt acattttgaa tggtattgtt attaattgta aataaatatt ggaaggtaaa
    19441 tgatatgaaa aatgaaattc aaaaaattat ggacaaatat gatccatggc atgaagatga
    19501 ttttgagtcg tatgaaaaca ttgccagaga tgtatcgttg atgacagata aaacgttcat
    19561 tgaacattat ttgttagaag tttattcaga agaaaacgga cattttgacc aagagaatgt
    19621 ccatgcaatg atagaagaaa ttaaaaatgc aatttagtga atatgtattt ttagaagaag
    19681 gctttgtgtc attttgatgc cgtctgaaag ggtttgttcg tttcagacgg cattgttacc
    19741 aaggcttgat tatttccggc gcaggtcggg atggttttcc aagttgtcca tcattatccc
    19801 gatgatgcgc ggggcggttt tgcccaaatc gaaacgttcg caggaagaga atcaccgcca
    19861 aatcagcccg tccaaggttg atttgatgaa gatgaccgcc gtttccttgt ccaaatcgtc
    19921 agccaaatcc tgattttcca ccgcttcggt caaaacggcg gtaattttct cgcgccagat
    19981 tgcctgatgc ttgcgggcaa tggcgataac ggcggcgttt tgctccgtgt gttcgcattt
    20041 taaaaacagg atgttgtgga atttgtagta gatgtcgttg ctttgcagcc gctcgaaaaa
    20101 gtgcagcagc gtgtggcgga ataccgccca agaccctcct tcggcatctt gcgcgatgca
    20161 gttttcgatg tcgtcgcaga tacgttggaa cagcgcgtcg aacaagtctt ccttattttt
    20221 gaaatgccaa tagagcgcgc cgcgcgttac gccggcggct tgggcgattt cgttgagcga
    20281 ggtgcgcgca atccctttgc ggtaaaaggt ttccaaggcg gcaagcatca ggtgttcttt
    20341 ggttttcaag gcttcggttt tggtttttct cataatggcg ttttcgtttc gggtcggttt
    20401 gatgagggcg gattataaaa aagactttgt aaccatgcaa tcgtgtatgt ataatgaaac
    20461 ccatgaaatt gagactacat ctcaactttg aaaacaggca taacctgctt tgcaacccgt
    20521 tcgaacattc gggtatcaaa gcaggcggtt tttatatc
//
LOCUS       DAXHMX010000020        20336 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHMX010000020 DAXHMX010000000
VERSION     DAXHMX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.020"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(199..274)
                     /locus_tag="ACFM4S_000542"
     tRNA            complement(199..274)
                     /locus_tag="ACFM4S_000542"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Asn,seq:gtt)
     gene            complement(367..726)
                     /locus_tag="ACFM4S_000543"
     CDS             complement(367..726)
                     /locus_tag="ACFM4S_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6050277.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(749..1393)
                     /locus_tag="ACFM4S_000544"
     CDS             complement(749..1393)
                     /locus_tag="ACFM4S_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6050278.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADV
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1395..1931)
                     /locus_tag="ACFM4S_000545"
     CDS             complement(1395..1931)
                     /locus_tag="ACFM4S_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6050279.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1943..3040)
                     /locus_tag="ACFM4S_000546"
     CDS             complement(1943..3040)
                     /locus_tag="ACFM4S_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6050280.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3043..4479)
                     /locus_tag="ACFM4S_000547"
     CDS             complement(3043..4479)
                     /locus_tag="ACFM4S_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6050281.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4699..5481)
                     /gene="bioC"
                     /locus_tag="ACFM4S_000548"
     CDS             complement(4699..5481)
                     /gene="bioC"
                     /locus_tag="ACFM4S_000548"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6050282.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVRLTYHPLFFIARYSAEGRQ"
     gene            complement(5469..6116)
                     /locus_tag="ACFM4S_000549"
     CDS             complement(5469..6116)
                     /locus_tag="ACFM4S_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6050283.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6126..7268)
                     /gene="bioF"
                     /locus_tag="ACFM4S_000550"
     CDS             complement(6126..7268)
                     /gene="bioF"
                     /locus_tag="ACFM4S_000550"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6050284.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVCL"
     gene            7491..8588
                     /locus_tag="ACFM4S_000551"
     CDS             7491..8588
                     /locus_tag="ACFM4S_000551"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC6050285.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGKGNLRLGN"
     gene            complement(8795..10261)
                     /locus_tag="ACFM4S_000552"
     CDS             complement(8795..10261)
                     /locus_tag="ACFM4S_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6050286.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10459..11382)
                     /gene="speB"
                     /locus_tag="ACFM4S_000553"
     CDS             complement(10459..11382)
                     /gene="speB"
                     /locus_tag="ACFM4S_000553"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6050287.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11481..13373)
                     /gene="speA"
                     /locus_tag="ACFM4S_000554"
     CDS             complement(11481..13373)
                     /gene="speA"
                     /locus_tag="ACFM4S_000554"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6050288.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13540..13824)
                     /locus_tag="ACFM4S_000555"
     CDS             complement(13540..13824)
                     /locus_tag="ACFM4S_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6050289.1"
                     /translation="MKKLLLVAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13975..15783)
                     /gene="aspS"
                     /locus_tag="ACFM4S_000556"
     CDS             complement(13975..15783)
                     /gene="aspS"
                     /locus_tag="ACFM4S_000556"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6050290.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGTKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15841..16542)
                     /locus_tag="ACFM4S_000557"
     CDS             complement(15841..16542)
                     /locus_tag="ACFM4S_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6050291.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16685..17833)
                     /locus_tag="ACFM4S_000558"
     CDS             complement(16685..17833)
                     /locus_tag="ACFM4S_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6050292.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17994..18257)
                     /gene="rpsT"
                     /locus_tag="ACFM4S_000559"
     CDS             complement(17994..18257)
                     /gene="rpsT"
                     /locus_tag="ACFM4S_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6050293.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18560..19702
                     /locus_tag="ACFM4S_000560"
     CDS             18560..19702
                     /locus_tag="ACFM4S_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6050294.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5244 a         5249 c         4721 g         5122 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagctg tccaggacag
       61 cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt ccatctcaaa
      121 acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct aagaaaatac
      181 ttagagggtt ttgaaatttg gctccccgac ctgggctcga accagggacc tgcggattaa
      241 cagtccgtcg ctctaccgac tgagctatcg gggaataagc gcgaataata aaacagaaat
      301 accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc ccgcaatctt
      361 tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca ataggattaa
      421 accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac ccgcctgact
      481 taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc cgcaggcgag
      541 ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc ctaccctgtc
      601 cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg agataacggc
      661 agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa tcaataattg
      721 ctgcataacc cctcctctct cccaagtttt acacgcggtt gctgtaacgg cggtataaaa
      781 tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg ataaccaaaa
      841 taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga ccggaaggat
      901 cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc agccggtatt
      961 tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc gcctcatgat
     1021 ggaaattggc gttggcaaga tacagaccgt tccgatcgac caataacgcc ttaccgctgc
     1081 cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg ccgtctgaat
     1141 gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac aggttcaaag
     1201 cggtatcgat gtcggcggtg tccgcccagg taagcagctt ctcactgctg accatttcgt
     1261 ccacatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg gcatcgctgg
     1321 atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct tgtagtgaaa
     1381 gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat tgcgctaacc
     1441 aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac attaagatta
     1501 tgttttgcaa gatatttgtg atacacgtcg ataccgggct gagagcgtat atccatcttg
     1561 gtaataccga caacgacggg tgccttctcc agcagccctc gaaacgaatg taaaaagaat
     1621 tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa acccatactg
     1681 ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt accatataaa
     1741 tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac tgtcgtattc
     1801 cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag tgcttcgtcc
     1861 gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa aataatttta
     1921 ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc ctttgcagaa
     1981 gaccgcgcga ctgggattgg gatggtgttg tgattttttc cgcttttttc atcatttcac
     2041 tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag tatgcctgtt
     2101 gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc gcaagataat
     2161 tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg ttgacggatg
     2221 ccttggtcag aaatgccgac aagcgtatcg accccggcac atttgccaac cgggtcaggt
     2281 ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc aacctgccct
     2341 gcgccgtcca aatggaaaac tgccacatac aggacataat gcctacttta gccttttcgc
     2401 gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg tctttgcaca
     2461 attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag gggaaaacaa
     2521 taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt tccgcaaacc
     2581 gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc gtacgctgca
     2641 cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc ccataaacat
     2701 tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga atcggtttgg
     2761 gcaggtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg ggtatatcct
     2821 tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg gtatccgcca
     2881 aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa cgggtggtat
     2941 tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc cccgccaaca
     3001 ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc cgtaccgttt
     3061 tattttttta atattttgat tcatacgctg cagcagccgg ctcatcagca taacgacctc
     3121 ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca acctatccca
     3181 atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgggact cgtcgggata
     3241 tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat acatcagcaa
     3301 cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttga tcaacgaaga
     3361 atcttgaagc accaaatccc gatgttgcga tttgaatttc tgtatatttt tcggcagata
     3421 cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt ccaaatcatc
     3481 gaaaacttca tggtaaccca agctgtaccc ccaccccagc atccgctctt taacctgcct
     3541 gccgtagtta cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac ggtctatgtc
     3601 cgcgtgttgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt tttcggaagt
     3661 ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt ctttaaggat
     3721 tttcgcacct tgatcggcgc gaaccaaacc ggcaatcgca ccgatttcct cttgactgat
     3781 tccggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt cggcttcata
     3841 agccttaccg ccggcatccg gcgggggcga tgcggaagct gccgtcgcag aaccgccttc
     3901 cgcacgtttt tcaatctcct gagtgcccca tcccaaacct tcttccgcca agacacggat
     3961 acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca aaagttcgga
     4021 aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat cccccacttc
     4081 gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac catccggaat
     4141 gccgtccaga taggcagcca aagattcggc agccttgccc tgataaccga attgcttata
     4201 aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg atgcggtctc
     4261 ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt gtccaatctg
     4321 atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc cgacaccttg
     4381 ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa gcatcagcaa
     4441 taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc gataccgtaa
     4501 cgagcggata gctgaccgcc tgcaacggcc attgtccgct tttattatta acgatttact
     4561 gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat atgccttctg
     4621 cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat ataaaaataa
     4681 ctttgcccat aagctgcctt attgcctgcc ctccgcagag tagcgcgcga taaaaaataa
     4741 cggatgataa gtcaggcgca ctttgcccga cggcatactg aacgccgaca agtaatcgcc
     4801 aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggcgg taacgcctgt
     4861 atatttaagg tgtttgagta catctaacgg cgtatcaaag tctagtatta ccttaaaatc
     4921 ctcacaccat aaaagctcaa aatctttggc taaccaagtc tgccattggg ataaagtcgg
     4981 gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt tgccaaaggt
     5041 tgcaaccgcc aataatccgt ttgctttcaa gcctgttttg caatgggtga taaaagcgtc
     5101 gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt gtcgttgaaa
     5161 aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt tttcagccag
     5221 ttggggctgc acatcgcaca aatcattaaa taaccaataa ttcgctgaaa tctgtttttg
     5281 cagcaaggca ctcaacattc ctgagccaca gcccaattcc aacacgtttt ccaatggcat
     5341 atccggcaaa taatcttgca aatgcgccat taaattaatc gtcatttttt gttggattaa
     5401 ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc gcgatttatt
     5461 tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa caggtaatgt
     5521 ccgccgtcaa tttcccgaac ggtgcaacgc ggtgtccaat atcggtgctg attggcaggc
     5581 ataaaaattt tatcgcccga tccgaccaag gcatttgtcc agcgaatcaa atctgcgcgc
     5641 ctatcttgcc cgatcatcgc aaaaagtgcg ataagttctt gatgaatttc gtcaaacgga
     5701 cgggcgggaa attgttggta atcttcaaaa gatgctttat cgccacacat tctgcgttca
     5761 aattttaaac gggtgttttc cgtgaggttc tccaatgtgc ctttaaaaac ggcgcaaggg
     5821 ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc ggattttaat
     5881 cttattcctt gcaatgccct ctctgccgcc caaacgccca ttgaccacgc caccaaacgg
     5941 atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat cttgataatc atagcaaatc
     6001 aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg cgttccccaa
     6061 cctgcaaaat acaggattaa atgtccgcct tgatgattgt aaaattttgt tttcatatca
     6121 tatccttaca ggcacaccgc aaactgccgc acttcatccg ttgtcatatc tgccgttaaa
     6181 gacaggcgga ttctggatgt gtttttgggt actgtcggtg gtctgatggg caggcaataa
     6241 taaccctgcc tttgcaggta ttccgctttg gcaagggtgg cttcattccc gcctaaaata
     6301 taggggacga tacaggtttg gctcggcatt atttgcgtcc gatgcgccac ttcccgccgt
     6361 aaaaatgcgc ttaactgctc aagatggctt ctttcttttg agaattgcgg caatcgttca
     6421 aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa aatcaatggg
     6481 cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata cgcccccacc
     6541 gaggctaagg ctttaccgaa agtgccgacc aataaatcaa tctcggcaat caaattatcc
     6601 cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgggc ttcatccaca
     6661 taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa atccgccaca
     6721 tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa ttttccgacg
     6781 tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg ggaaaacgca
     6841 caccggctca aacggatgcc gtcaatcata ctggcgtgaa caaatttatc tgccaaaatc
     6901 aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat tggcgtgata gccgctgttg
     6961 aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa atcggtataa
     7021 ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg aaaattaccg
     7081 ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc caaataatca
     7141 ttagacgaca tattcagcat tttgcggttt tcccgcgtaa tataccgccc ttgatgaatc
     7201 aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg ttgtttaaaa
     7261 accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag catcaaacaa
     7321 tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga cctttgccac acccctgcct
     7381 tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac ccaatccgtc
     7441 cgcactgtac cgaagcgcat attttcgcac aaattggtac ggtttcaggt atgatttgcc
     7501 gaaaacctat cggacacaca ccatattttg atatgaataa aacaatctgc cgcactgctg
     7561 cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc tcccccgtcg
     7621 cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc tacaccgacg
     7681 gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa accaatctag
     7741 gcgaacataa aatcggtgcg aaagcctata accctgtttg gtacatcccc aaatccatac
     7801 agaaagaacg cggcgacggc gtaaaaacca tagccgccgg cccggacaac cctttggggc
     7861 cggtttttgt ccgcttaggc gaccctaaac tcggtttggg catacacggg accaatgcgc
     7921 cggccagcgt ccccggggtt cggagtcacg gctgcgtccg catgaaatcg cccgacgcgc
     7981 tcgagtttgc caaaaccatc gccagcggct cgcccgcctc cgtcatctat caaatggcgg
     8041 gtctcaatga agatgcggat cgcaacctgt ggcttgccgc cttccgcgac ccttacggta
     8101 agaacaacct tgacatcgcc tctctgaaaa aaagtattgc gcaatgggca aaaacacagg
     8161 gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa agaccgcacc ggatcggccg
     8221 tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc gctcaaatcg ctggcgtgga
     8281 tacagggttc ttcctcatac agccaagctg aagtcattga acaaactgag gaaaccaact
     8341 ctgccgaaat atccgagaca cgcacgcccg aagtgcctga tgtacacacg cccgaggcac
     8401 aaccgcattt aaatacccaa tccgacggca cgccgactgc ctataccgaa ccggctgccg
     8461 attcatcgcc gcaagtagaa acacctgatc aggctgcttc cgagccggtt gatgtattat
     8521 tttcaataga tgtgatacgg cagggaaatt tgcgtttagg taagggaaat ttgcgtttag
     8581 gtaattgaat aaccttctga ttatttaatg cttatgttta tcagaagttg ataggcggtt
     8641 tggtttttgt ttggcttttc ctacaccgcc gcctgccgct ttctgcaaca ttcaagcgca
     8701 caaatatgcc atctgaaggc tttagacggc atatttcacg ataatagtcg cccaatcaaa
     8761 taaacacaga ttggaaattt attttatttt tcccttacca ataccccaga actttccacc
     8821 aaatgctgcc gataacgaaa aaaatcagaa aattgactac gctcatgata aaacccgcct
     8881 tccaccattc tcccattgtg gtgtagcccg aaccgaaaat cacaggcgaa gtaccggtcg
     8941 cataatgagt gagggtcatc ataatgttgg atgcggccgc catcatcagc gcggtcggca
     9001 tcgcaggggc attcagtgaa acggcagcag cgagaaatgc gccgaacata gcggtaatat
     9061 gtgcagtagt actggcaaac atataatgcg catacatata agcaagcacg aggattacgc
     9121 ccgcagccgt gccgctaacg cccaaaccgc cgacactttc cgccaacact ccggagaacc
     9181 atttaatcag tccgagttta tttaaaaatg cggccatcat aatcaatgcg ccaaaccaaa
     9241 taatcgtatc ccacgcactt ttttctttca aaacatcgtc ccaagtcaat acaccggaaa
     9301 gcaaaagcag gcttaatccg ataaatgcgg tggcggtggc gttgatacta aaagcgtgat
     9361 tgccggtaat aagggcggga acatctgccc acaacagcaa caagataccg aaaatgaccg
     9421 ccataatgat ttcgtctgcc gacattttac ccatctccct cagacggtct ttggcaaatt
     9481 gaacggcatt gggcgtttct ttaatttcag gcggatacaa aaaatataaa atcaaaggca
     9541 taacgaaaaa ggcgataacg ccgggaacag ccattgccca cgcccacgcc ccccaagaaa
     9601 gacggaaact actgcctaaa ttttcggcaa tcaagttgac gattaaaggg ttgggggcag
     9661 ttgcagtaat aaacatagcc gacgaaatgg gattggaatg atagttgacc aaagccaaat
     9721 atttacccat cttgccttct gtgccttttg cgggattgga gccgtaactg ccggcaatcg
     9781 actgcataat cggatgtata atgccgccgc cgcgcgcggt attggaaggg gtaacgggag
     9841 ccagcagcag ttcggaaaga gcgagactgt aaccgatgcc cagcgttttt cttccaaaaa
     9901 cggcgataaa caaatatccg atacgcatcc ccagccctgt tttgagcaaa ccgcgcgaaa
     9961 tcataactgc gatggcaatc agccaaatca acggattggc gaacgcactc aacgcatcgc
    10021 tcatcgccgc gcccggtttg tcggcggtta cgccggttac tgcgaccaac ccgacggcaa
    10081 taatcgacag cgcgcccaac ggcatggcct tgccgataat ggcggcaatc acaccgacaa
    10141 acatggccag cagcgtccaa gcctgaggct tgaccccgtc gggtacgggc agtgccaaaa
    10201 ccagggcgca caatactgcg gcaatggcga ggggtatcgg tttgaaaccc aatttcatca
    10261 tattgacctc cgtaaaaaag accgtcccga aaaatcggaa aaataatatt taactaattg
    10321 ttttataaga tatattccga tatttcaccg tctttccgat atgcggctcc gggcaacttt
    10381 tgtttcagta tttgaatttt cattagactg aatacgccgt ttgaacggca cggcgaaaac
    10441 ccgggggatg ccggacgttc agtccttttt cgcaccttga aggtaaagca tttccaaggc
    10501 aattgtggcg ccggccaaag cggtaatgtc ggattggtcg taagaggggg caacttctac
    10561 aacatccata ccgacgatgt cgagatccgt cagcccacgt aggattttta atgccctgtc
    10621 gctgctcaag ccgccgcata cgggcgtacc ggtcccaggg gcgaacgacg ggtccaggca
    10681 gtcgatgtcg aaagtcaggt aaacgggcat attgccgacg gtttctttga ttttacggac
    10741 ggtctcttca acactgtctt cattgacttt aggggcggtc aacacagtaa aaggcaattt
    10801 tttactgtgt tcggtgcgta tgccgatttg tacggaacgg gacgggtcga tgaggccttc
    10861 cttgggggcg gtatagaaca tcgtaccgtg gtcgtattcg ctgccgttgt cgtaggtgtc
    10921 ggtgtgcgcg tcaaaatgaa tcagtgcgag tttgccgaaa tagcgggcgt gggcgcgcaa
    10981 caacggtagg gtaatgaaat ggtcgccgcc caaactcaaa cagcgtttgc cggaagaaag
    11041 taatttgccg gcgtgcgctt ccattttttc gacaaaatcc ctgctgtcgc caaaagaaaa
    11101 aaccaagtcg ccgcaatcaa taatgttcag gcgttcgcgc acatcaaatg tccatggaaa
    11161 cctgcggtgc tcccaagcga ggttgacgga ggcgcgccgg atggcttcag gaccgaaacg
    11221 cgcgccggaa cgccctgaaa ccgccatatc ataaggcacg ccggtaataa cccaatcggc
    11281 atgactttca tacggcataa aattaagcgg caggcgcaaa aacccgaaat tattggaaac
    11341 gagggagttg tcggtttgtc ctgccagtgt gctgtattgc atcgtaatga ttccttgtaa
    11401 ttggtttcaa tcggtcgtga tgattggtgt ttgagtaaga aaatcgggct tcagacgaca
    11461 tatccgatgc cttgatgcgt ctattcgtct tccaaatagg tataaccatt aagccccgct
    11521 tcgagttctt ttaagaaaga catagcctgc gaggcaggaa ggtctgaatg ttcgatttgt
    11581 tcgcgatagc gtttcatcag ctctttcgga tcttgataaa cgtattcgag catatcggca
    11641 acggtgtttc cttcatcgta atcgatgacg gtaaattgtc cgtcttcccc tacaacaaca
    11701 tcggcagtgg cagtgtcgcc gaaaagattg tgcatattgc cgagtatttc ctgatatgct
    11761 cccaccataa aaaagcctaa aagcggcggc tcttcttcgg gataatcagg cataggcatc
    11821 gtaccggcga tgccgtcgcc gtcgatgtag tggtcaatcg taccgtctga atcgcaggta
    11881 atgtccaaca acacggcgcg gcgcgcaatc ggttcattca aaccggtaat gggacaaaca
    11941 gggaaaagtt gatctatgcc ccaagcatcg ggcaaagatt ggaagagtga gaaattgaca
    12001 tacagcttat cggcaaaacg ttcttgcaat tcgtcaataa tggttcggtg agaccggtgt
    12061 ttttcattaa acaattcgcc gacttcatga cagatattta aatacagttg ctccgcccac
    12121 gcacgttgcg ccaaactcaa cagcccgaca ttatactgat tatgcacatc ggcaagatca
    12181 aactgccctt cgtgtatcca gctgcgtaag gaacgttttt cccgcgaggc ggaaatatcc
    12241 gtccaagttt cccacatact gtgcaacaca cgcggtgctt cgggcgatgg cgcatccagc
    12301 cggcgcggtt tgtaacgttc aacgcctata acattagcaa ccaaaacggc gtgatgtgcg
    12361 gtaatgccgc gcccgctctc ggtgatgatt gtcggatgcg gcagcccgtg ttcgagacaa
    12421 gcctggctga tgccccatac gactgtggcg gcatattcgt tgaggctgta attaacggaa
    12481 caatccgatt gtgtgcggtt tccttcgtaa tccacgccaa gcccgccgcc tacatcaaaa
    12541 cagcggatat ttacccccag tttgtgcaac tcaacataaa accgagccga ttcgtgtaca
    12601 cctgtggcaa catcacggat gttcccaagc tgcgagccca aatggaaatg caaaagctgc
    12661 aggcagtcca gcctgttttt ttgtttcaaa atatcgacca gttgcaaaac ttgggaagcc
    12721 gacaagccga attttgattt ttccccaccc gaagactgcc attttcccga accttgggaa
    12781 gccagtctgg cgcgcacacc caaacggggc ttgatgccga gtttttccgc ctcttccaat
    12841 accatttgta tttcggacag cttctcaatc accaaataaa cctgatgccc cagtttttcg
    12901 cccatcaagg cgaaacggat atattcacgg tctttatagc cgttgcagac gattaatgtt
    12961 tgccgggtgc cggcgtgtgc caaaaccgcc atcagttcgg ctttagaacc agcttccaaa
    13021 ccatgcggtt gtccgcttga cataagcgat tcgatgacgc ggcggtgttg gttgaccttg
    13081 atagggtaaa ccaaacaata accgccctta tagccgcact cttcccgtgc cgtctgaaag
    13141 gcgcggttaa tgtcgcggag gcggtgttcg aggatttgcg gaaaacaaaa caaaacaggc
    13201 aggcgcgcct gatgtttttg ttgcacggct tcagtcagtt tttgcagtga aacagtttga
    13261 ttgtgttgcg agggattggg gcggacgatg atttcgccgg aatcgtcaac atcataataa
    13321 cctatgcccc aatgattaat gttgcacact tcacggatgg taaggatagg cataataaac
    13381 ctgctccgtc tgtcgtgttg aaaaggaatg attataacaa atcagcgtga aatgtcattt
    13441 ttttaataag aaaagcctgc ctcatacctg atgagaaaca ggcaaaatgc cgtctgaacg
    13501 cttcagacgg cattttggtt cagctttcca tcaaaggaat cagcgatcga gctgctcttt
    13561 gatgattttc aggtcggggt aagacaacac gatgtcgtat tcgcggccgt ctttataggc
    13621 ttccacttcc aaaacaggtt tgccccaatg gtcgtcggca tcgacatcgt gaacctgata
    13681 accgcgctgt tccaacattt tcacggcttt tgtgcggttt tgttcaaaat ggggatcgcc
    13741 gtaaatctga cgctcggcag agtcgccggc aaatgcggct gcggcactca gggaaacaac
    13801 ggcgaccaat aacagttttt tcattttcag tccttttttt atcggttgat tgaacaagat
    13861 gtgtttttca ataccgccat taaaacacag caaaattagg tttgaattag agggaagtca
    13921 agaagcgtaa atgccgtctg aaaaaaacag agccgtcaaa cggctctgtt ttccttatgc
    13981 ttctttggtt tcagccgcct tttgacgcag gcgcaaactc aattcgcgca actgcttgtc
    14041 gtccacgctg ttgggcgcgt tggtcagcag gcattgggcg cgttgtgttt tcgggaaggc
    14101 aatcacgtcg cggatagatt cggcgccggt catcagggta accaaacggt cgaggccgaa
    14161 tgcaaggccg ccgtgaggag gcgcaccgaa tttcaggttg tccaagagga agccgaattt
    14221 ctcttgttgc tcttcagggc tgattttcag cgcggcaaac actttctctt gtacgtctgc
    14281 acggtggata cggatagagc cgccgccgat ttcccagccg ttcaatacca tgtcgtaggc
    14341 acgggccagg cagtttgcag ggtcggaaac catcaggtct tcatgacctt ctttaggcgc
    14401 ggtaaacgga tggtgtacgg caacgtagcg gtcggcttct tcgtcgtatt cgaacattgg
    14461 gaaatcgaca acccacaaag gtttccattc gtctgtgaag tagccgttat ctttgccgtg
    14521 ttccaagccg actttgatac gcaatgcgcc gatggcttcg ttcacaactt tggttttgtc
    14581 tgcaccgaag aagataatat cgccgttttg tgcgccggta cgcgcgatga tttccttcag
    14641 ggcattctca gacaggaatt ttacgattgg agattgcagg ccgctgtctt cgccgttgga
    14701 gaggttgccc acatcattta ctttgatgta tgccagacct ttcgcaccgt agatgccgac
    14761 aaatttagtg tattcgtcga tttctttgcg gctgaatttc gtaccgttcg gcacacgcag
    14821 ggcaaccacg cggccgcctt tcatgtcggc tgcgccacgg aagactttga attcttccgt
    14881 tttcatcagg tcggtcaact cggtgaattt caagttgata cgcatgtccg gtttgtcaga
    14941 gccgtagtag aacatggctt cagagtaagg catgcgtggg aagtcgccca agtccacgcc
    15001 cagcgcgtct ttgaagactt gtttcgccat accttcggtg atgtccatga tttcatcctc
    15061 gtttaagaac gaggtttcca agtcgatttg cgtgaattca ggctgacggt cggcacgcaa
    15121 gtcttcgtcg cggaagcact tggtgatttg gtagtaacgg tcgaaacccg ccaccatcaa
    15181 cagttgtttg aacaattgcg gcgattgcgg cagcgcgaag aactcgcccg gatgaacgcg
    15241 gctcggcacg aggtagtcgc gcgcgccttc gggcgtggag cgggtcagca tcggggtttc
    15301 aatgtcgatg aaaccttgcg cgtccaagta gcggcgaacg cccatagcga cttggtaacg
    15361 caggcgcagg ttgcgctgca tcaccggacg gcgcaggtcg ataacgcggt tggtcaggcg
    15421 aacgttttcg ctgatgtttt cgtcgtcgat ttggaacgga ggcgtagcgg cagcgttcaa
    15481 cacttcgatt tctttggcaa ggatttcgat tttgccggaa atcattttgt cgttggtcgt
    15541 gccttcggga cggttgcgta cgcggccggt aatgctcaaa acgtattcgt tgcgggagga
    15601 atcggcagcg gcaaacgctt cgggcgtgtc gggatcgatc acgacttgga cgatgccttc
    15661 gcggtcgcgc aggtcgataa aaatcacacc gccatggtcg cgtcgacggt gtacccagcc
    15721 tttgacggta acggtttggt ctaagtattg ctcgctaatc agaccgcaat agttggtacg
    15781 cataaaatca ccttttattg atttaaactg aaaacagaaa atgccgtctg aacggcggct
    15841 ttattgttgt tcgggcaaat ccgccttttc agacggcata ggtcctgcca atgttttgac
    15901 gggcaggtcg tcagggatga ccatacccag cgaaatcaca tatttcaacg cttcgtccac
    15961 gctcatatcg agttcgcgca catcgctttt ctttaccata atatagtaac cgccggtcgg
    16021 attcggcgtg gtcggaacat acacggaaag ataatcgccg tccttcggca atgcggcctt
    16081 aaccgcattc gacacctgac cggacacgaa tgcgattgtc caaatacccg attggggaaa
    16141 cggcacgagt actggtgttt taaacgaacg gctgctgtcg gacagcaacg attcggatac
    16201 ttttttcaca ctcgaataga tggacttcac aaccggaatc cgccccaaca agctgtccca
    16261 cgcggcaaga atctgccggc ccaatacgtt tgcggcaaac aatccggtta caaacaatac
    16321 ggcaatggca acgataacgc ccagccccgg gatattaaac cccaaaacat attgcggccg
    16381 ccattgcttc ggcagcaggt tgacgagctg atcggacgcg gaaacgatat aggaaaccac
    16441 ccaaaccgtt accgcaatcg gcagccaaac caaaatgccc gtaatcagat atttttttaa
    16501 cgccttggca gctttaccgc cttcggccgc aggttccgtc atcttgcttg attccgacaa
    16561 agtccgtaca aaccgcacat tatacgcgtt tgcccggatt caaacgaaat ttttatcccg
    16621 ccccgccaaa ccgccggcgg cttcagacgg aacggcaact tgatataccg tctgaacacg
    16681 cggttcagat gccgtccaag tcgttgaaca tcaacccgat accgataccg ttctgcttgt
    16741 ggttgtagtc gatcaggctc tcgccgtaac cgtggaatcc gcgtaccacg cctttgagtt
    16801 tgcccttaat cggaaacgtg taggcggctt caatcgcgcc gtagcccgtt ttggggttgt
    16861 agcgcaatac ggaatacaca ttctgcctgt cgttcaggcg gtactgcagc ttcacgtcgc
    16921 cataccccat atagtcggca atatcgggat tgtcgttttt atcgccgctc tgatcgaacg
    16981 cacgcaccca cacgcgcgga atcaccgtca atttgcccca ttccatgcct gccatggcgt
    17041 aaatcctgtt ccacgaacgc gattcgggac ggctctgtcc gttggactgg tggacaaaac
    17101 ccgcaccgag catacgcagc ctgccgccga acggcaaatc cgccttcaca ggctgggtca
    17161 ggaaaatttc aggtttgtaa tccgtattgc ggaacggcgc ggatttcctg ccttggttgt
    17221 aaatctgcca atcggatctt tgggtgtagc cgaaccacag atccgcgcgg gttttaaaca
    17281 aatcttcggc aattttgctt ttgaacgaaa cctgcaattt ggtttccgca cgtttctgct
    17341 gtccgaattt ttcctgtaca gtcgtaccgc gcgtcggcga actcggggca tagttgggcg
    17401 aattgttgta ccagagcggc ataaggtaca tcggattgtg ttcgcgtacg cccaacagcc
    17461 cgcgcaaatc gtttttgtcc aagtcgtaca tcaggctcaa aggcgtatag atgtcggcgg
    17521 tttcgcccgc actgtcggca ggaagcgcat ccccgccttt ttcaacaaca atgaccgcct
    17581 cgcccttatc caggctgctg cggacggttt ccgtcagatt gagtacagct ttcgactcct
    17641 gcccttcctg tcctgccgaa gacggaagct gtgccgcaaa aatcctgtcg taacacgcca
    17701 aacgcgtaac attgtccgtc aaagcggcgc attgcagcgc ggtctctcca aaagcggatg
    17761 ccatcggcaa cagtcctgtc aaaagaatat agcgcatatt ccgtgtattc atctccgccc
    17821 ccattgtcgg catattggtt ttcaaacggc attttatagc ggattcggat aaaaaaccgc
    17881 accctttccg ccatttcggg atttagcccc cgtaatacag aaaaccccga aaccgtcagg
    17941 cttcagggtt ttccgcttat cgcgtatcaa ccgccttggc ggttttgcaa aaatcaagcc
    18001 agtgctttta cttttgcaga cagacggctt ttgtggcgtg ccgctttgtt tttgtggaac
    18061 acgcccttgt cggcgatgcg gtcgatgact ttgacggact cttggtaaac cgcttgtgcg
    18121 gcagctttat cgcctgcttc gactgctttc aatacttttt tcactgcagt gcggaatgcg
    18181 gtacgcaggc tagcgttgtg ggcgcgttgt ttgacggact ggcgggcacg tttgcgtgct
    18241 tgtgcgctgt ttgccatatt ggaatctcct ggaagaaaaa taaattctgt aaacgcgcaa
    18301 ttttaaaggg ggtttacatt atatgcaagc cttttctctc tatccgcccg tcggacaccc
    18361 tgacagcgca aaaaaacggc aaaatcgtgc ggacgttttc cccctctttt gaaaacaggg
    18421 taatttttta gggaaatcag tccaatatca ctttgagttt gcacaaattt actttacaat
    18481 gcccaaacct tgcgccgggg tgtttatggg gcgcggggtt ttcttttttt ccaacctctt
    18541 tcatcaggaa tctgcccgta tgaaaaaatc cgtattagcc gttttggccg cgctgtctct
    18601 ggcagcctgc ggcggcagcg aaaaaaacgc cgtgcaacct caagcagacg ctgcgtctgc
    18661 tgccaatgcc gaagccgctg ccaccgatac tctgaacatc tacaactggt cgaactatgt
    18721 tgacgagagt acggtcgaag actttaaaaa agccaacaat ctgaagctga cttacgactt
    18781 gtacgaaaac aacgaaacgc tggaagccaa aatgctgacc ggcaaatccg gttacgattt
    18841 ggttgtgccg ggcatcgcct tcctgccgcg ccaaatcgag gcgggcgcgt atcaaaaagt
    18901 caacaaagac ctgattccca actataaaaa catcgatccc gaactcttga aaatgctgga
    18961 aaccgccgac ccgggcaacc agtatgccgt cccctatttc tccggcgtga acacgattgc
    19021 gattacggcg aaaggcaaag agcttttagg cggcaagctg cccgaaaacg gctgggattt
    19081 gctgttcaaa cccgaataca cccacaagct gaaatcctgc ggcatcgccc tgtgggacac
    19141 cccgagcgaa atgttcccga ttttgctgaa ctacttgggc aaagacccca aaggttcgaa
    19201 tcctgaagat ttgaaggcag cggcggaagt gttgaagtct atccgtccgg atgtcaaacg
    19261 tttcagcccg tccatcatcg acgagctggc gcgcggcgac atctgcctgg cggcaggcaa
    19321 cggcggcgat ttgaacttgg cgaaagcacg ttccgaagaa gtgaaaaaca acgtcggcat
    19381 cgaagtgctg aatccgaaag gcatgggctt ctggattgag tcttggctga ttcccgccga
    19441 tgcgaaaaac gtcgccaatg cccacaaata catcaactac acgctcgacc ccgaaatcgc
    19501 ggcgaaaaac ggcatcgccg taaccttcgc ccccgccagc aaaccggcgc gcgaaaaaat
    19561 gcctgccgag ctggtgaaca cccgttccat cttcccgaac gagcaggata tgaaagacgg
    19621 ttttgtgatg ccgcaaatga gtacggatgc gaaaaaactg tctgtcagcc tgtggcagaa
    19681 aatcaaagtc ggcaccaact gatttgaagc attaaaaatg ccgtccgaac atcgttcgga
    19741 cggcatttta tattggattg aaatagaaat atttatatag tggattaaca aaaatcagga
    19801 caaggcgaca aagccgcaga cagtacaaat agtacgaaac cgattcactt ggtgcttcag
    19861 caccttagag aatcgttctc tttgagctaa agcgaggcaa cgccgtactg gtttttgtta
    19921 atccactata ccgtcgttcc cacggtcagg atttcagatt gcggacatct gtcagaaaag
    19981 acaaaaaacc ttccgccgtc attccccaca ggcgagaatc cgatccgttg aaattcggtt
    20041 gttttaaata aattcttaca gctttgattt tctgtttttc cgataacgcc gtaactttga
    20101 aacgcgaaag cggtaatccg atccgttggg attttgcaac ttcaaatcaa tccgcaaact
    20161 gaaatcccgt cattcccgcg cagtcgtgaa tccgaacgcg tccgcacgaa aacctgcatc
    20221 ccgtcattcc cacggaagtg ggaatctagg acgtaaaatc tcaagaaacc gttttacccg
    20281 ataagtttcc gcaccgacag acctggattc ccgcctgcgc gggaatgacg aaattt
//
LOCUS       DAXHMX010000021        19526 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHMX010000021 DAXHMX010000000
VERSION     DAXHMX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19526
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.021"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            47..1225
                     /locus_tag="ACFM4S_000561"
     CDS             47..1225
                     /locus_tag="ACFM4S_000561"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6050295.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASIASV
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMEKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(1292..1540)
                     /locus_tag="ACFM4S_000562"
     CDS             complement(1292..1540)
                     /locus_tag="ACFM4S_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6050296.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(1631..2548)
                     /gene="ftsX"
                     /locus_tag="ACFM4S_000563"
     CDS             complement(1631..2548)
                     /gene="ftsX"
                     /locus_tag="ACFM4S_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6050297.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(2545..3195)
                     /gene="ftsE"
                     /locus_tag="ACFM4S_000564"
     CDS             complement(2545..3195)
                     /gene="ftsE"
                     /locus_tag="ACFM4S_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6050298.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(3192..3383)
                     /locus_tag="ACFM4S_000565"
     CDS             complement(3192..3383)
                     /locus_tag="ACFM4S_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050299.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            complement(3391..3873)
                     /locus_tag="ACFM4S_000566"
     CDS             complement(3391..3873)
                     /locus_tag="ACFM4S_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6050300.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            3997..4350
                     /gene="arsC"
                     /locus_tag="ACFM4S_000567"
     CDS             3997..4350
                     /gene="arsC"
                     /locus_tag="ACFM4S_000567"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6050301.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            4347..4991
                     /locus_tag="ACFM4S_000568"
     CDS             4347..4991
                     /locus_tag="ACFM4S_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6050302.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPFLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFN"
     gene            5195..6589
                     /gene="gltX"
                     /locus_tag="ACFM4S_000569"
     CDS             5195..6589
                     /gene="gltX"
                     /locus_tag="ACFM4S_000569"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6050303.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFERYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNEKLAELVKPRLA
                     LRDIHETEKPALEDVLAQVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            6610..7005
                     /locus_tag="ACFM4S_000570"
     CDS             6610..7005
                     /locus_tag="ACFM4S_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050304.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDYAWNAVYGAGGTKASGN"
     gene            7009..7500
                     /locus_tag="ACFM4S_000571"
     CDS             7009..7500
                     /locus_tag="ACFM4S_000571"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6050305.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(7623..10217)
                     /gene="mutS"
                     /locus_tag="ACFM4S_000572"
     CDS             complement(7623..10217)
                     /gene="mutS"
                     /locus_tag="ACFM4S_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6050306.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPAIHIENGRHPVVEQQVRHFTANHTNLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPRKALSELYRL
                     KDLCKSVS"
     gene            10659..11663
                     /gene="gap"
                     /locus_tag="ACFM4S_000573"
     CDS             10659..11663
                     /gene="gap"
                     /locus_tag="ACFM4S_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6050307.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(11733..11852)
                     /locus_tag="ACFM4S_000574"
                     /pseudo
     CDS             complement(11733..11852)
                     /locus_tag="ACFM4S_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11921..12601)
                     /gene="tsaA"
                     /locus_tag="ACFM4S_000575"
     CDS             complement(11921..12601)
                     /gene="tsaA"
                     /locus_tag="ACFM4S_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6050308.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            12716..13351
                     /locus_tag="ACFM4S_000576"
     CDS             12716..13351
                     /locus_tag="ACFM4S_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC6050309.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDY
                     CVWKGIDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            13388..14356
                     /gene="waaC"
                     /locus_tag="ACFM4S_000577"
     CDS             13388..14356
                     /gene="waaC"
                     /locus_tag="ACFM4S_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6050310.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            14583..15332
                     /locus_tag="ACFM4S_000578"
     CDS             14583..15332
                     /locus_tag="ACFM4S_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC6050311.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            15343..16278
                     /locus_tag="ACFM4S_000579"
     CDS             15343..16278
                     /locus_tag="ACFM4S_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6050312.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAAAVQGGN
                     AEVINIETTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            16341..16952
                     /locus_tag="ACFM4S_000580"
     CDS             16341..16952
                     /locus_tag="ACFM4S_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6050313.1"
                     /translation="MITTYKTITSPTQAGFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVSGLIHAYKEATAQALAIAEVIEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEVCVAAWKNTRQIEVNTEKPFE"
     gene            complement(17004..17321)
                     /locus_tag="ACFM4S_000581"
     CDS             complement(17004..17321)
                     /locus_tag="ACFM4S_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6050314.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            17479..18750
                     /gene="purD"
                     /locus_tag="ACFM4S_000582"
     CDS             17479..18750
                     /gene="purD"
                     /locus_tag="ACFM4S_000582"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6050315.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGKLDSVAAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            18784..19386
                     /locus_tag="ACFM4S_000583"
     CDS             18784..19386
                     /locus_tag="ACFM4S_000583"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6050316.1"
                     /translation="MTDMLQTAFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         4718 a         4441 c         5463 g         4904 t
ORIGIN      
        1 cattaattgt ttctgtatcg caaacactga ttgaaaagga ctgattatgg catttttaaa
       61 actgaccgaa caaaacgtgc agggcaaaac cgtcctcatc cgcgccgata tgaacgtgcc
      121 gttcaaagac ggcaaaatca gcgacgacac ccgtatccgc gcctcgattg cgtccgttaa
      181 atactgcgtg gacaacggcg cgtccgtcat cgtgatgacc cacttgggcc gcccgaccga
      241 aggcgagttt caccccgaag acgacgttgc gcccgttgcc gcgcatttgg gcggcctgtt
      301 gggcaaaaac gtgaaagtat tgaacgactg gcgcgaaaac aaaccggctt tgaacgcggg
      361 cgatgtcgtg atgctgcaaa acgtgcgtat caacaaaggc gagaagaaaa acgatttgga
      421 actgggcaaa gcctacgctt ctttgtgcga cgtgttcgtc aatgacgcgt tcggcaccgc
      481 gcaccgcgcc caagcctcga ccgaagccgt cgcccaagcc gctcccgttg cctgcgccgg
      541 cgtattgatg gcgggcgaac tcgacgcttt gggcaaagcc ttgaaacagc ccgcgcgccc
      601 gatggttgcc atcgttgccg gcagcaaagt gtccaccaag ctgaccatcc tcgaatcgct
      661 ggcggacaaa gtcgaccaac tcatcgtcgg cggcggtatc gccaacacct tcctgttggc
      721 ggaaggtaaa gccatcggca aatctttggc ggaacacgat ttggtggaag aatccaaaaa
      781 aatcatggag aaaatggcgg ccaaaggcgg ttctgtgccg ctgccgaccg atgtcgtcgt
      841 tgccaaagcc tttgctgccg atgccgaagc ggtcgtgaaa gacattgccg acgttgccga
      901 agatgagatg attttggaca tcggcccgaa atccgccgcc gcgcttgcgg agttgctcaa
      961 agccgccggc acggtcgtat ggaacgggcc ggtcggcgtg tttgagtttg accagttcgc
     1021 aggcggtacg aaagcccttg ccgaagccat tgcccaaagc aaagcattct cgattgcagg
     1081 cggtggcgac acgctggcgg cgattgctaa attcggcgtt accgaccaaa tcggctatat
     1141 ttctaccggc ggaggcgcgt tccttgagtt cttggaaggc aaagagctgc ctgccgtagc
     1201 tgctttggaa aaacgcggcg cgtaagcggt ttgatataaa aaatgccgtc tgaaacgttg
     1261 tgctgtttct gacggcattt ttgtgtggcg gttattgtgc tttggctgcc cattccgccg
     1321 gagtggcggc aaccgaaatg gaaagtgcgt gcagttcgtt actttccagt tgggctttga
     1381 gtccgtcttt aatcaggcgg tggcgcgcga ggcgtgcctt gccttcaaat tctgatgaaa
     1441 cgatgacggc gaaaaaatgg tgtccgtcgc cttctacttc gatatgttcg cattttgcca
     1501 cgccttcaat catggctttt acttgttccg aggtcagcat ggtaggttcc ttgttggaaa
     1561 aatgcgccat tatactggca aattgaaatg ccgtctgaaa acgggttcgg acggcatttt
     1621 tgacggtgtt ttattttttg gctttgaagc cgagcaggtg ctgggtggtg gcaagccacg
     1681 cgccgaatac gcccaacgcg atgacgaagc cgaacaccag tgcgatttct cccgggtaga
     1741 agaaacgcca gccgatatta agtccgtagg gtttgaagat ggcatcgacc aaggggcgca
     1801 cggcagagag cagccaaccg caaagcccca agctgacggc ggcggaaagg atgctctgcc
     1861 acatggcttg ataaaggaat gggcggcgga taaacgacgc gggcgcgccc aagagtttgg
     1921 tgatttcgat ttcttctttg cggctgagga tttgcagtcg gatggtgttg tgcgcgacaa
     1981 ggacgaacgc catccccagc gtcagggaaa gaaaccataa gattttacgg atgaattcgt
     2041 tgatttggta cagtgtctgt acccattccg tatccatgtt ggcggattcg accataggca
     2101 gtttggtaat gtcccggtag attgcctgca tttgggcggg cgtggttgcc gggtcggggg
     2161 taacgataaa gacatccggc agggggttac cgtcaagcat ggaaatcaga ttttggtcga
     2221 gattggactg taattcttcc agaccgtctt ctttgcctat gaagcggatg ttgtcgagcc
     2281 gtttgtcgcg cgccagcagg ctgcggacgg tatcgctgtc gctttgtgcg gcggcggttt
     2341 ccatatagat tgtgatttgc ggcgactcat tgagtttgcc caacacgctt tgcccgcttt
     2401 ggatgcccaa atacataaac agcggcaggg tcatggcgac ggcgagcatt atgagggtaa
     2461 gcagtgtgcc gaaaggttgg cgcagaagct gtttgagcgc ggtgcgcgcg gattcgacgt
     2521 gcagcgagag gtagtggatg atgctcatgc gagtcgtcct ttcgagaggc gcaggatgcg
     2581 gtgtccgtag tccgccatca gggtttcgtc atgtgcggcg acgatgacgg ttgttcccgc
     2641 ttcgtggaag gttttgaaca attccataat atcgagcgcg taggcgcggt cgaggttggc
     2701 ggagggttcg tcggcaatca gcaggccggg ctggtgaacg acggcgcggg cgatgcacag
     2761 gcgttgttgt tcaccgccgg agagggttac gggatcgtcc aattctcgtc ctttcaggcc
     2821 gactttttcg atggcgatgc gggcacgctc ttcggctttg cgcggcggat agccgataat
     2881 ccgaagcggc aggatgacgt tttgcaggac gttgcggtcg tagaggattt tgtggtcttg
     2941 gaacacgatg ccgatgtgtt ggcgcataaa gccgatttgg ttgtcggaca atgtgccgag
     3001 gtcctgcccg ttaaacagga ttttgcccct gctcggcttg gtgatgcccg aaatcagctt
     3061 gaggacggtg gatttgcccg aaccggagtg tcccgcgata aatatcattt cgcctttgtt
     3121 gatttggaag ctgacgtttt tcagggcttc aaaaccgccg ggataggttt tggaaacttg
     3181 ttcgaaacgg atcataggag gtcctgtaag gaatgggcgg catatcgttt cgggcggttg
     3241 cgtccgcagg gatatgccgt ctgaacaata ggtaagacag tgccgattat aaagattccg
     3301 cctgttttta gatagtggat aagtttggcg gataccgaag gtcgggcggg aaaaatgccg
     3361 tctgaagcgg attcagacgg catccggcgt ttaacggcat tttgaatggg cgagtttgac
     3421 ggcttcggtc aggctttttt cgttcacttc cccggtaatg gtttgtttgt atccgcattt
     3481 cggggcttcg acgacggtaa agggcagtac gccgacattg tttccgtagg atttcataaa
     3541 gtttcggctg ttcgccccgg tgtaacgcca aatcgggtag gaaacagggg tttgcttgag
     3601 gaagttgccg atattgtcgg atgtgtcgag cgcgatgccg accatatcga cactgccttt
     3661 tttctgcgct ttgtaccatt tggacatggc aggcatctct tttcggcacg ggccgcacca
     3721 ggttgcccaa aggttgacga tgcgtacggg ggctttgagt gattgcaggc tttgcggggt
     3781 gttgtctttc catccggcca gttcgtctgc cgaggcggta tgtgcggcaa atgtggcggc
     3841 aagggtgatt gcggcaagat tcaagtattt catcgttttg tcctttgtat gtcgattcgg
     3901 gtattgtaaa ccgtttggta cggttgtagg cgggatattg agtaaaaccc tgtgtaagcc
     3961 gataataagg ggattgtcga gaagtaagga aatgtcatgt ctgaaatcaa aattttccac
     4021 aacccccgtt gcagcaaatc gcgtgccgcc ttgtccctat tggaagaacg cggcattgct
     4081 gccgaagtgg tcaaatattt ggatacgccg cccgacttgt ccgaattgaa ggatattttc
     4141 aacaaattgg gcttggcatc ggcgcgcggg atgatgcgcg tgaaagatga tttgtataag
     4201 gaattgggtt tggacaaccc caatttggac aatgacgcgc tgctgcgtgc catcgccgat
     4261 catcccgccc tgttggagcg tccgattgtt ttggcgaacg gtaaggcggc tgtcggcagg
     4321 ccgttggaaa atatcgaagc ggtattgtga acaaacggct gttttgcagc cgcaacggct
     4381 tgcgctatta cctgttgggc ggtttctgtt tgagtgtttt tccgtttttg ctggtgtttg
     4441 cctcatccgt ttgggcggtt tatcggacgg gcgggcaggt tttgccgccg tatgtccggg
     4501 cggatgccgc gctggtgttg ggcgccgccg cgtgggacaa acgcccttct ccggtttttc
     4561 gggaacgcat caaccacgcc atcgcgcttt atcagagccg cagggtaggc aaaatcgtgt
     4621 ttacgggagg gcgaaccaaa aaaggctata tgaccgaagc cgaggtcggg cggcggtacg
     4681 cgctcaaaca gggggttccg gcgcgcaaca tcctgtttga aaacacttcg cgcaacactt
     4741 atgaaaacct gaacaacatc cgtccggttt tgcgtgccaa cggtattgcc agcgtggtga
     4801 ttgtcagcga tccctaccat ttggcacggg cggcggaaat ggcggaggat ttgggtgtcc
     4861 gcgtttatat gtctgccacg ccgaccacgc ggtttgatgc gggaaataag aaaaaaatct
     4921 ttatgctgca agagggatat gcgttatccc tctaccgttt ggaaaaatgg gggcgggatt
     4981 ttttcaatta gctgtttcat tgaacttttt tttggacgca atcccgaaga aatccggtcg
     5041 gcggcgcgtg atacactgtt ccgcgtgccg aagcattgaa aacaaggaaa atgccgtctg
     5101 aaacttatcc gcaggcaggg ttcggacggc atttttaatg cccaagcacg gtaaaaacag
     5161 taaaataagc cgttttaaag attctggagc catcatgacc gtcaaaaccc gtttcgcccc
     5221 cagccccacc ggctacctgc acattggcgg cgttcgcacc gccttgtttt catgggcgtt
     5281 tgcccgccat cacaaaggcg agttcctatt gcgtatcgaa gacaccgact tggcgcgttc
     5341 caccgccgaa tccgtcaaca tcatcctcga cggcatgaaa tgggtcggtc tcgattacga
     5401 taacgccgac aacgtggtgt accaaacccg ccgcttcgaa cgctataaag aagtcatcgc
     5461 cgaactcttg gcaaaaggcg atgcctacta ctgctattgc agcaaagaag agctggaagc
     5521 gatgcgcgag aaagccgaaa aagaaggcac ggcgacttac gaccgccgct ggcggccgga
     5581 agcaggcaaa accctgcccg aaatccctgc cggcgtgcaa cccgtcgtcc gcttcaaaac
     5641 gcctttggat ggcgtaacca agtggacgga cctggtcaaa ggcgaaattt ccatccccaa
     5701 cgaagccctc gacgacctga ttattgcacg cgccgacggc acaccgacct acaacttctg
     5761 cgttgtcgta gacgactacg acatgggcgt tacccacgtt atccgcggcg acgaccatgt
     5821 gaacaacacc ccgaaacaaa tcaacatctt aaaagccatc ggcgcaaacc tgcccgaata
     5881 cggtcatctg cccatgattc ttaacgaaca aggcaaaaaa atctccaaac gcagcggcga
     5941 tactgttgcc attaccgatt tcggcgcgat gggtatcctg cccgaagcca tgctcaacta
     6001 tctggcgcgc ttgggctggg cgcacggcga cgacgagttc tttacgatgg aacaattcat
     6061 cgaatggttt gatttgaaag acgtttcccc gtccccaagc cgtatggatt tgaaaaagct
     6121 ctattggatc aacggcgaac acatcaaaat cacgcccaac gagaaactcg ccgaactcgt
     6181 caaaccgcgt ctcgctctgc gcgatatcca tgaaaccgaa aaacctgcgt tggaagacgt
     6241 gttggcacag gttaaagacc gcgcccaaga tttgaacgcc ttggcagacg aatgtctgta
     6301 tttctacaaa aaacaagtcc ctgccgaagc cgatgttgcc aaacactggg acgacgaagc
     6361 cgccgctcgt atgctgcgct ttgccgaacg cctcgagggt ttggaagact ggaatgccga
     6421 agccatccac gacctcttca aacctttctg cgacgaagaa ggcatcaaaa tgggtaaact
     6481 cggtatgccc ctgcgcctcg ccgtctgcgg tacggcgaaa accccaagcg tcgatgctgt
     6541 gttggcatta atcggcaaag aagaagtgtt gaaacgcatc cgcgcttaag ttatccacag
     6601 gcgtgggcga tgccgtctga tgtcggaatg cggcttcaga cagcattcaa acggaagttg
     6661 aacatgaaaa aaatattgta ttttctgatg tttgtttttt ctacaagcgt atgggcgggg
     6721 gatgcggaag acaatctgct cagcatccaa tccggctatc gtgccttact gcaaaaacaa
     6781 aacaatctgg acggaaaaat catcgggatg cagtcggatt tggaagatgc gcgccggcgt
     6841 ttgcagacgg ctcaggcaga catcgcccgt ttggaagcgg aaattcctgc agcaatggcg
     6901 caaaaagccc ggcaggctga agagctgagg caaatcggag tgcgtttgga ctatgcttgg
     6961 aatgcggttt acggcgcagg gggaacgaaa gcgtcgggga attgaccgat gaattccctc
     7021 tttgtggaca atactgtttt cattacacgg ctgaaagccg ggcatatcgg caggttggtt
     7081 caggcgttgt ttgaggagtg gcacggattt gaaccgtggt cttctgtgga taagattcat
     7141 gcctattacg gcaggtgttt gaaggatgac gaactgccgc tggcttttgc ggctgtggat
     7201 gattccggaa ccctgttggg ttcggctacg gtcaagcggc atgatatgga aagttttcca
     7261 cagtatgaat attggttggg tgatgtcttt gttttacctg aatatcgcgg aaaaggcatt
     7321 ggcaggaggc tggtcgccca ctgcataggc gcagcgcgtt cgctggggat aaagttcttg
     7381 tatctttata cgcctgatgt gcaaatattt tatgaatcat tcggctgggc ggttgtcggg
     7441 cgacatttcc ataacggtga atgggttacg gttatgcgtt tggatatgga taaggtttaa
     7501 gccgaatgga caaatgccgt ctgagctgtt cggacggcat ttttatgttt ggaaacttct
     7561 tgtcagataa tgtttatcca cagatttttc catatggttt gatgctgttc cgccaacgga
     7621 aattaagata cggatttgca caaatctttc agacggtaca gttctgacaa tgctttgcgc
     7681 ggggtcaggc tgtcgggatc gagtttttcc aaggctgctg ccaatatacc ttcaaaatgt
     7741 ttttcctctg ctttatccac aaagttgccc acattcggtt catctccttt ttcagacggc
     7801 atggtactga aaatatccag ttggggacgg ttcgcggcgg cttggttttc cagttcgttc
     7861 aaatgctttt gggcggattt caatgcgcgt acaggcaggc cggcgagttt ggcgacggca
     7921 atgccgtagc ttttaccggc gggacccggt tggatttggt gcaggaaaac gatgtcctgt
     7981 ccctgttcga gcgcggaaag gtgcatattg acggcggcgg cgtgggcttc gggcaggtag
     8041 gtcagctcga aatagtgggt ggcaaacagg ctgaaggatt tgtttttttg cagcaggtgt
     8101 tcggcaacgg cgtgcgcgag ggcgaggccg tcgaaagtgg aagtgccacg tccgacttcg
     8161 tccattaaaa caaggctttg ttcggtggcg tgatgcagga tgtaggcggt ttcgctcatt
     8221 tcgaccatga aagtggagcg gttggaggcg aggtcgtccg atgcgccgat gcgggtgaag
     8281 atttgatcga tgggcccgat tgtggcggca tcggcaggca caaaacagcc ggtgtgtgcc
     8341 aataaaacaa tcagcgcgac ttggcgcatg taggtggatt tgccgcccat attggggccg
     8401 gtgagcagca tgaggcggtg tttgtggtca aggttggtgt ggttggcggt gaagtggcgt
     8461 acctgctgtt cgacaacggg atggcggccg ttttcgatgt ggatagccgg atagtcggca
     8521 aactcggggc ggacgaagtt ccgctctttt gccaaggctg aaaatgtgga caatacgtcc
     8581 agcgcggcgg cggctttggc ggctttttga agctgcggca atgccgtctg aaggtttttc
     8641 aatacgccgt caaagagttg tttttctaag gcgagggctt gctcttgagc agtcagcact
     8701 ttgtcttcaa aggctttcaa ctccggcgtg atgaagcgtt cggcgttttt gagggtttgc
     8761 cggcgttggt agtcggcagg tgcttgttcg gcttgggttt tggacaattc aatgtaaaag
     8821 ccgtgaacgc ggttgaactc gactttgagt gtggacaaac cggtacgttc gcgttccttg
     8881 gcttccaaat ccagcaaaaa ttcgtcgcca tggttttgaa tgcggcgcaa ttcgtccagt
     8941 tcgggatgaa aaccgtggtt gatgacattg ccgtctttca gccagacgga aggttcgggc
     9001 aaaatggctt ggcggagctg ttcggctgtg gataggtttt ccgggaaaac ggctttgagg
     9061 gtttccaaca ggctgctgcc gtttgcggac aagtcgattt gtgccaactc gaacagactg
     9121 tcgcgtaaag atgccaaatc gcgcgggcgg gcgttgccga cggcgatgcg ggcagcaatg
     9181 cgttcaatgt ccgcaatgtt tttcagacgg cattggaggg gtttgtattg gctttccagc
     9241 gcggcaacgg cttcttggcg cgctcggatg tgggcgcggt tgcgtaaagg gtggtgcagc
     9301 cagagagcca agaggcggct gcccatatgg gtagcgcaaa ggtcgagcgt ggacatcagg
     9361 gtcggcgatt ttttgccgga gagggtttgc gtgatttcga gattgcggcg cgtggcagca
     9421 tccataccga tatattggct gtcggtttcg agcgacaggc cgtccaaatg ttgcggcatc
     9481 aggttttgcg tcagacggat atagttcaac agtgcacctg ccgcgccaat cgcaacggcg
     9541 tgttctttgc cgtccaaacc gaagccgcgc aaatcctggc agccgaaata ttccgtcagc
     9601 agtttttccc ccgcgtcggc ggcaaactgc cacgcgttca ggcgcgtaac acccgatgcc
     9661 gtctgaagtt gcggtgcgtt tttactgtcc ggcaacagaa tttccgccgc ctgcaggcgc
     9721 gccagttcgt cgtccaattt atccacagtt gtcagcttgg ttttgaattc gccgctttgc
     9781 agcgatgccc acgccaaacc gatgtatttt ttgtcggggg acacggcaac gatacggttg
     9841 gtttccttgt cttccagcaa tgcggaatcg gtcagcgtgc cgggcgttac gatgcgcacg
     9901 actttgcgct ccacaggccc tttgcccgcg ccgacttcgc cgacctgttc gcaaatcgcc
     9961 acgcttttgc ccaacttgac taggcgcgcc agatattgtt cggcggcgtg aaacggcacg
    10021 cctgccattt tgaccggctc gccatccacc tgtccgcgcg tggtcagggt aatatccaaa
    10081 agttttgccg cttctaccgc atcgtcgaaa aacatctcgt aaaaatcgcc catacggtaa
    10141 aacaccagtt tgtcggtatg ttgcgctttg atgccgaggt attgctgcat cattggggaa
    10201 acggcggatt tggacatggg gctgccttat cattaggaaa acggtattgt agcaaaaggt
    10261 tttgtcttcg acggaatgag actgaccgta agcctgtcaa taaggttgtg gagacaacta
    10321 ggaaaacagc aaagttatcc acagaaatat tattaagtca aaaaacatcc acgcttatct
    10381 gtcttataca gcaagtctta aactcaaact tatttgacaa aaagataatg aaaatattgg
    10441 atatttgcta cttacgcaca agcaggttgg caatcatcaa aatcatcatt aaaatttaaa
    10501 ttcaaatatt gatggtaaag gttttggaaa tctgtgcaaa cagcaaaatt catctatatc
    10561 aaataagcgt caagtagtta gatgtaatag tggaatgtaa ctttatttca gaatacaatg
    10621 caggcatcgg actttatttc acaacctgga gacaaaatat gagcattaaa gtagcgatta
    10681 acggtttcgg ccgcatcggc cgcctcgcat tgcgtcaaat tgaaaaagcc cacgacatcg
    10741 aagtcgtcgc cgtcaacgac ctgacccctg ccgaaatgct gctgcacctt ttcaaatacg
    10801 acagcacgca aggccgtttc caaggtactg ccgaattaaa agacgatgcc atcgtggtaa
    10861 acggcaaaga aatcaaagtt tttgccaatc cgaatcccga agaattgcct tggggcgagt
    10921 tgggtgtgga tgtcgtcctc gaatgtaccg gcttctttac caataaaacc aaagccgaag
    10981 cacacatcag ggccggcgcg cgcaaagtcg tgatttccgc acccggcggc aatgatgtga
    11041 aaaccgtcgt atatggcgta aaccaagaca ttttggacgg cagcgaaacc gttatctccg
    11101 ctgcttcctg caccaccaac tgccttgccc cgatggcggc agtcctgcaa aaggaattcg
    11161 gcgtagtcga aggcctgatg accaccatcc acgcctacac cggcgaccaa aacacccttg
    11221 acgcgccgca ccgcaaaggc gatttgcgcc gcgcccgcgc cgccgcgctc aacatcgtgc
    11281 ccaacagcac cggtgccgcc aaggccatcg gtctggttat ccctgaattg aacggcaaac
    11341 tcgacggctc cgcccaacgc gtccctgttg ccaccggctc gctgaccgaa ttggtttccg
    11401 tcctcgaacg ccctgtaacc aaagaagaaa tcaacgccgc gatgaaagcc gccgccagcg
    11461 aatcttacgg ctacaacgaa gatcaaattg tttcttccga cgttgtcggc atcgaatacg
    11521 gttcgctctt cgatgcgacc caaacccgcg tgatgaccgt gggcggcaaa caattggtga
    11581 aaactgttgc ctggtacgac aacgaaatgt cctacacctg ccaactcgtc cgcactttgg
    11641 aatactttgc aggcaaaatc taaggcttga acaaacctgt ggataagtaa atgccgtctg
    11701 aacacattgc gttcagacgg cattgtttat ctgtatagtg gattaaattt aaaccagtac
    11761 ggcgttacct cgccttgccg tactatttgt actgtctgcg gcttcgttgc cttgtcctga
    11821 tttaaattta atccgctata acttgatatt gcggcttatg gcgaacaaac aacagccgtt
    11881 tgttcaactg ctatgcggca aaaccggatt tttgcccgat ttaaagcggg gttggggaaa
    11941 gatcaataac ggttgcacgg ttttcctcga tttgaaatct gacttcgtaa tctgcaatat
    12001 tcatcacata aatccgttcg ggaatattct gataggcggg gcgcggatct tgggcaatgc
    12061 tttggctgat aaggtttttg gtgtttgcag ataaattttc cgcgccgatg ttttcctgcc
    12121 aaacgacttc caactctacg ggtttgccgc tgacgaaacc agatgcggca tcgggtttgg
    12181 attcgacaaa ggggatataa ggcttgatgt ccacaatcgg cgtgccgtcc agcaggtctg
    12241 cgccgctgca atagaggcgg acgggtttgc cggtttcgat gcgttcgagt ttcaggagcg
    12301 agagtccgag atggttgggg cggtgggggc tgcgcgtggc gaatacgccc attttttgtt
    12361 tgccgccgag ccgtggcggg cgcaccattt gtgcccagcc ttcatccaat acgccgtgaa
    12421 aaataaaact tatccacaca tagtcgaagt gttccagtcc gcgtacgctg tctgcggtaa
    12481 attcgggatt cagctcgatg catacttctg cggcagagac caaaccgggc tggcgggcga
    12541 tgccgaattt ctgtttgtag ggcgatcggg cggtggcgat agggacgatg gtgtaagtca
    12601 tgaggattgt ggataagttt ttcggaaaaa ccttatctta tcacagcctg ttttaccgct
    12661 tttgggcagt aatttgttat aatacagggc attttaattg cgttgaaatg atgagatgat
    12721 tgtttccatt gatgttgatg cacaaaaaac ttttacgcct ttgtgtccta aagagctgcc
    12781 tgtaaacgag gggcatttga ttgtcgaaga attgaatgcc caagccgctt tggcggattt
    12841 gcgcgtgatg acgaaagatg cgcatcatgc ggcagcaaaa tggcttgtgg ataaccctgt
    12901 tgatatgttg aagccgacag gtttgcccga tgcggatttg acttgggtgg ctcatgcgat
    12961 ggttggtacg cgcggctatg aattgttgga cgggctgcct tgtgccaaag aatacgatta
    13021 ttgcgtttgg aaaggtattg atcctgaatt gcatccttac ggcgcgtgtt ttcacgatat
    13081 tgaggaaaaa ctaagcacag ggctgattga atggctgcgt tgtcaaaata cggatacggt
    13141 tattatcggt gggttagcta cggattattg tgttaaaaca acggttttgc agttactcaa
    13201 aggcggtcgt tggcaggttg tcgtcaatga agcggcttgt cggggtattg cgccggaaac
    13261 catcgaaaca gcatggcagg aaatgcattc tgccggggca atcatcttaa aaaacgctga
    13321 tgaaataaat aaatatatta ataatcaata agttaaaatt gtttcacgtg aaaccccctt
    13381 cctaaatatg aaaattttgc ttgtccgctt gtctagtatg ggcgatttaa ttcacacttt
    13441 gcccgcaatc gaagatttgg cgcgacaatg tcctgatgta gaactgcatt ggctatgtga
    13501 ggctggattt gcagatattg cgcgcctgca tccgtttgta aaaaaaatcc atgtgatgaa
    13561 atggcggcaa tggcgcaaac atctctttcg ggctgaaact tggcgggaaa tgggtcatct
    13621 gaaacagact ttgcggcagg aagtatttga tttcgtattg gacagtcaag gtctgattaa
    13681 aagcgcgtgt ttcgctaaaa tggcgaaatc ccctatttat ggtttggata aaaacagtgc
    13741 gcgcgaggga tgggctgctt tggcgtatgt aaaaacatac gctgtaccga agggaaaaaa
    13801 tgccgtttgg cgcaaccgtg aactgtttgc ccaagtattt gggtatgtaa tgccggaaac
    13861 gcaggtattt ggcttgactg ttcctgaagc aggccgtctg aaaaatttag cgcagccgta
    13921 ttatgcggct ttgcatgcga ccagtcggga cagtaagtta tggcctgtgg aaaactggcg
    13981 gtcgttgctg caaaagttga atgaagaaca gcaatgcaat atttacctgc cttggggaaa
    14041 tgaagatgaa aaaacgcgtg ccaaacaaat tgcagacgga ctgccgtttg ctattgtgtg
    14101 cgacaaaata aatttattgc aggcagcgta tctgctgaaa tacgcggtcg gaattgtcgg
    14161 cgtggatacc ggtttgctgc atttggcaaa tgccttggaa aaacctgtgg tcggcattta
    14221 taccgatacc gatccgatta aaacaggcgt tcaggtttcg ccaattgcaa aaaatttggg
    14281 caatatcggg cagattccga ccgcagattt ggtttatcaa gcgctgatgg attgtgttgc
    14341 agcagacaaa ggctaaaggg ttgtctgaaa ctgatatggc ggcatgatat tttccaattt
    14401 attaaataca ggaatgtgta tccgctagtc tgagtaaatg ctttaaaaat ggtataatcg
    14461 gcaagcatta tccgaaagat ttttgaatga attcgttttt aaggaaaacg gatttggttt
    14521 tatagggaag aaggtttggt ttcgctggat ggtgaaagca gcgttctttt taggagggtt
    14581 caatgaaagc actggtcgca gtaaagcgcg tagtggacta caacgtcaaa gttcgtgtaa
    14641 aagccgatgg ttcggatgtg gatatcggca atgtcaaaat gtcgatgaat ccgtttgacg
    14701 aaatcgctgt ggaagaagcc gtccgtttga aagaagccgg aaaagtaagc gagatcgtag
    14761 cggtttcttt gggcgaaaag aaatgcgaag aaacgttgcg taccgctttg gcgatgggtg
    14821 ccgaccgtgc aattcatgtt gaaaccgatg cgaaattgga gccgctggca gttgccaagt
    14881 tgctgaaagc cgttgcggac aaagaaaatc cgcagatttt ctttttgggc aaacaggcga
    14941 ttgatgatga tgccaaccaa gtggcgcaaa tgctggctgc tttgctgaat gcggcgcaag
    15001 gtacgttcgc gtccaaagta caaattgaag gcgacgaagt gcagattgtg cgggaaatcg
    15061 atggcggcga agaaaccatc gcattgaaac tgcctgctgt tatcagtgct gatttgcgtt
    15121 tgaacgagcc gcgctttgtc aaactcccca atattatggc ggcaaagaaa aaacctctgg
    15181 aaaaactggc gcctgccgat ttggctaccg atattttacc tcgtctgaaa acggtgaaat
    15241 ttgccgaacc taaggcgcgt caggcaggcg taaaagtagc aagcgttgcc gaattggttg
    15301 aaaaattgaa aaacgaagcc aaagtgattt gaggaggctg aaatgagcgt attgattatt
    15361 gtcgaacacg acaacaaaca gttgaatcct accactttgc atgctgttac cgctgccgcc
    15421 aagctgggta aagtcgattt attggttgcc ggaagcggtg catctgccgt agtagagtcc
    15481 gcaaaacaag tggcgggtgt ggaaaaagtt ttggttgcag atgctgccca ttatgccgaa
    15541 ggtttggctg aagagttggc tccgctggtt gttaaattgg cagcggatta ccgctatgtt
    15601 gcagcaacgg caaccacatt cggtaaaaac cttttgcccc gcgtagccgc cttattagac
    15661 gtaccgcaaa tttctgattt gaccgagatt gtagataaca cgacttttgt acgtccgatt
    15721 tatgcaggta atgcatttga aaccgtgcaa gccaatgcgg aaaaattggt gctgaccttc
    15781 cgtgcgacgg cttttgacgc agcggcggtg caaggtggta atgctgaggt aattaatatt
    15841 gaaacaaccc ctgcccaaaa cctgagtcgt tttgttaacc gtcagctttc ccattccgat
    15901 cgtcccgaac tgactcaggc aaaagtgatt gtttctggtg gtcgtgcgtt gggtagtgcg
    15961 gaaaaattta atgaagtgct gacaccgttg gcggatgttt taggtgcggc aatcggtgca
    16021 tcccgtgcgg cagttgatgc cgaatatgcg ccaaacgatg ctcaagtcgg acaaaccggt
    16081 aaagtagttg cgccgcaact ctattttgca atcggtattt caggtgcgat tcaacacgtt
    16141 gccggtatgc aagacagtaa ggtgattgtt gcaatcaata aagatgctga tgcgccgatt
    16201 ttcaatgtag ccgattacgg attggttggc gatttatttg aaatcgttcc acaattaaca
    16261 gaagcattga aaaattaatg tttcacgtga aactttcaga cgactttgtt attaatgagg
    16321 ttgtctgaaa acttttcaac atgattacga cttataaaac cattacttcc ccgacgcagg
    16381 ctgggtttaa agataaaggc agccgtttta tcgcatttgc ctatccgatt cggacattgg
    16441 ctgatgtgaa aaaatacctc gatccgttaa aggaagagca ccataaagca cgacactggt
    16501 gttatgccta tcgtttgggc gtggatggta tgcaatttcg tgccaacgat gatggagagc
    16561 catcaggaag tgctggacga ccaattttgg gacagattga ttcggtgggc atcaccgatg
    16621 ttttggtcgt ggtcgtccgc tatttcggtg gtactttatt gggcgtttca ggtctgatac
    16681 atgcgtacaa agaggcgacg gctcaggcgt tggcgattgc ggaagtgatt gaaaagaata
    16741 ttgaaaaaac cgtttggctg aaatgtgaat atccattttt gaatgaagcg atacgcatcg
    16801 ccaaacaata tcaggcggat atattggagc aggatttgca gttggattgc cgattgacgg
    16861 taagtttgtc gttggcaaat tatgaagtct gtgttgcggc ttggaagaat acacggcaga
    16921 ttgaagtaaa tacggaaaag ccttttgaat gaaaggtcgt ctgaaatgct ttgtgatggt
    16981 atttcagacg acctcttcgc tgactaatct tcttgtgtat atttgtctaa ggcatcgacg
    17041 ctggagcgca aatataacca agccagttga tggaattcgc ccagcgcgtt ccatagcgca
    17101 tcttcactca taatgctgta ttcgccttct gtgcgcaaat ccacctccgc tccatcttca
    17161 atagcccgat gacaagcctg atattcgtgg gtataaaaat tgtccatatc gcgaatcaaa
    17221 gcgacggcat gtttccaacg gcggatgtct tcttccaatt gcggtagtaa atgacaccat
    17281 tcgcggtatt tgctttggat ttcatcaata cgtttttgca tggttgcttg tcctttttta
    17341 atttgtgaat aagttgaata ggttgcagag tggaatgaag cggtcttttg tttgattcta
    17401 ctaaaatttc ttcagatggg ataaaagtcc ctttgtatcg agttttcgtt aaaagtacat
    17461 atttgtcaag gagatggcat gaaactgctg gttatcggta atggtggtcg cgaacacgcg
    17521 ctggcttgga aattggcgca gtcgcctaaa gtggaaacag tatttgttgc gcctggtaat
    17581 gccggtacag cgattgaacc caagttgcaa aacatcgact tgactgcgca tcaggatttg
    17641 attgaattct gtcgtaaaga aaatattgtt tttactgtcg tcggccctga agcgcctttg
    17701 gcggcaggtg ttgtggatga tttccgtgcc gcaggactaa aaatattcgg tccgacacaa
    17761 tatgcggcgc agttggaaag ttccaaagat ttcgccaaag catttatggc gaaatacaat
    17821 attccaaccg cgcaatatca aacctttgaa aacgccgatg ccgcacatga ttacgtcaat
    17881 cagaaaggtg cgcctatcgt tatcaaagcc gatggtttgg cggcaggtaa aggcgtgatt
    17941 gtggcgatga ctttggatga agcgcatgct gcgattgatg atatgctgct ggacaacaaa
    18001 atgggcaatg ccggcgcgcg tgttgtgatt gaagatttcc tgcaaggcga agaagcgagt
    18061 tttatcgtca tggttgatgg caataatgta ctgcctatgg caaccagcca agaccacaag
    18121 cgtcttttag acggcgacaa aggtctgaat acgggcggta tgggtgcgta cagccctgcg
    18181 cctgtggtaa cgcctgttgt gtacgaacgc gcgatgaatg aaattatttt gccgaccgta
    18241 gcagggatga aagcggaggg gcatgagttc accggtttcc tgtacgcagg tttgatgatt
    18301 gatcaaagcg gtgcacccta tacgattgag tttaactgcc gtttcggcga tcctgaaacc
    18361 cagccgatta tgagtcgtct gaacagcgac ttggcggatt tggttgaagc ggcaatagac
    18421 ggtaaacttg atagtgttgc tgccgaatgg aatccacaaa ctgccgtagg tgtagtgttg
    18481 gcggcgcaaa actatcctga aacgcctaaa aaaggcgatg ttatttccgg tctggatgct
    18541 gccaaccagg taggcaaagt tttccatgca ggtacaacgg caaacgagaa aggtgacgta
    18601 ttgaccaacg gcggacgcgt attgtgtgtt gtgggattgg gcgacaatgt ggcgcaagcg
    18661 aaagctaagg catatggcgc gttggaaaaa atcagcttcg acgggatgca ataccgtaaa
    18721 gacattgccg ataaagcgat taaccgttaa ttagaaagag gatgatgccg tctgaagcca
    18781 agtatgactg atatgcttca gacggcattt tatattatgt tatttccgag aattatcgct
    18841 gcatcagcgc aacgtaagtt aagcgtttat ttaaaaaaag gcggtttggt cgcttatccg
    18901 accgaatctt gttatggctt ggggtgtctt ccgacattag ccaaagcact cggtaagttg
    18961 gcgcatttga aaaaaagacc gcaacacaag ggtatgattg tcatcgggaa tcaatttgaa
    19021 caattgcagc ctttacttca aatgccgtct gaaaatcttc aggatatgct tagaaaagaa
    19081 tggcccgccc ctaaaacgtt cctattatcc gccaaatcct gtgtcttacc tgagttgcgc
    19141 gggaaacagc gcagcaagtt ggcggtcagg gttcccgccc atgtaggtgc gcgccgcttg
    19201 tgtcaggctt tgcaaacgcc tttggtttcg acttcgtgca accgcgcagg caaacgcgcg
    19261 tgtcggacgg agagggaagt cagaaggcaa ttcggacgcg atgtgtggat tgttggtggg
    19321 agaatcggca ggcagaaatc gccgagtcag attattgacg gggaaacagg taaacgtttg
    19381 cgttgagacc tttgcaaaat tcctttccct cccgacagcc gaaactcaaa cacaggtttt
    19441 cagctgtttt cgccccaaat accgcctaat tctacccaaa taccccctta atcctccccg
    19501 gatacccgat aatcaggcat ccggtc
//
LOCUS       DAXHMX010000022        18391 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHMX010000022 DAXHMX010000000
VERSION     DAXHMX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18391
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.022"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            138..572
                     /locus_tag="ACFM4S_000584"
     CDS             138..572
                     /locus_tag="ACFM4S_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC6050317.1"
                     /translation="MNFALSVITFTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(621..1964)
                     /locus_tag="ACFM4S_000585"
     CDS             complement(621..1964)
                     /locus_tag="ACFM4S_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6050318.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTYTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIRLANDSPFGLGACIYTADTGCA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1969..2622)
                     /locus_tag="ACFM4S_000586"
     CDS             complement(1969..2622)
                     /locus_tag="ACFM4S_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6050319.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQVRPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2773..3063
                     /gene="gatC"
                     /locus_tag="ACFM4S_000587"
     CDS             2773..3063
                     /gene="gatC"
                     /locus_tag="ACFM4S_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6050320.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3126..4571
                     /gene="gatA"
                     /locus_tag="ACFM4S_000588"
     CDS             3126..4571
                     /gene="gatA"
                     /locus_tag="ACFM4S_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6050321.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVQTA
                     LQNTINLLKTQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4568..5500
                     /locus_tag="ACFM4S_000589"
     CDS             4568..5500
                     /locus_tag="ACFM4S_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6050322.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5543..6973
                     /gene="gatB"
                     /locus_tag="ACFM4S_000590"
     CDS             5543..6973
                     /gene="gatB"
                     /locus_tag="ACFM4S_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6050323.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7065..8075
                     /locus_tag="ACFM4S_000591"
     CDS             7065..8075
                     /locus_tag="ACFM4S_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6050324.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRKNGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8204..8836)
                     /gene="pdxH"
                     /locus_tag="ACFM4S_000592"
     CDS             complement(8204..8836)
                     /gene="pdxH"
                     /locus_tag="ACFM4S_000592"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6050325.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9067..10116
                     /locus_tag="ACFM4S_000593"
     CDS             9067..10116
                     /locus_tag="ACFM4S_000593"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6050326.1"
                     /translation="MSKQPTSKRQWRDGVAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10185..11726)
                     /locus_tag="ACFM4S_000594"
     CDS             complement(10185..11726)
                     /locus_tag="ACFM4S_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6050327.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTICVPADGWK
                     PEGYIAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(12019..13374)
                     /gene="xseA"
                     /locus_tag="ACFM4S_000595"
     CDS             complement(12019..13374)
                     /gene="xseA"
                     /locus_tag="ACFM4S_000595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6050328.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAEERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGTGSEFQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKDTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            13571..14392
                     /gene="nadE"
                     /locus_tag="ACFM4S_000596"
     CDS             13571..14392
                     /gene="nadE"
                     /locus_tag="ACFM4S_000596"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6050329.1"
                     /translation="MMRIFSTGGTMDTQAVITHIARWLDEYATQANAKGFVIGVSGGI
                     DSAVVSTLAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTADLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANAKSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(14463..14936)
                     /gene="dnrN"
                     /locus_tag="ACFM4S_000597"
     CDS             complement(14463..14936)
                     /gene="dnrN"
                     /locus_tag="ACFM4S_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC6050330.1"
                     /translation="MTDFSVWETAPFGATVDYILQRYHNVHRAQFEELVPLAQKVVQV
                     HADTFPAEIARLLADMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            15234..15566
                     /gene="trxA"
                     /locus_tag="ACFM4S_000598"
     CDS             15234..15566
                     /gene="trxA"
                     /locus_tag="ACFM4S_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6050331.1"
                     /translation="MSSELIIHTSDAAFEKDVLNSDTPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(15619..16566)
                     /locus_tag="ACFM4S_000599"
     CDS             complement(15619..16566)
                     /locus_tag="ACFM4S_000599"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6050332.1"
                     /translation="MTDITPFANRLGKNIKHLMKWVKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMLDILDIQRDHKKLIDGAVKLLASDGILYFSNNLSSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            16894..18282
                     /locus_tag="ACFM4S_000600"
     CDS             16894..18282
                     /locus_tag="ACFM4S_000600"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6050333.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         4630 a         5380 c         4646 g         3735 t
ORIGIN      
        1 gggaatctgt ttcaccccaa tatataaagc accatattaa ggcggagttt cttccccact
       61 ttgacccgaa cccggaaaag acaccgccca agccaatcct gatgctgccc cgacagccaa
      121 cccttaagga aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt
      181 tcctgcccgt cccgcctgcc ggagccgccg tctttacttg gaaggacggc ggcggcaaca
      241 gctattcgga tgtaccgaaa cagcttcatc ccgaccaaag ccaaatctta aacctgcgga
      301 cgcgccaaac caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg
      361 gcgcggcaca ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa
      421 agaaaagaat tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa
      481 tgaacctgaa ggcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat
      541 acaataacgc cgtaaacaaa tactgccgtt gagcagcctc atcgaaaacc ccatgccgtc
      601 tgaagcggca cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac
      661 agcccgaatt cggacaattc gcgcccgtaa ccggaatctt taacccctcc gaaaggcagg
      721 cgcaaatcgc tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca
      781 aaccgccagg cgcatccggt atcggcggta taaatgcagg caccgagccc gaacggggaa
      841 tcattggcaa ggcggatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc
      901 ccgaatactt cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg
      961 ggataaaacc agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt
     1021 gaaacggcat cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt
     1081 gcaagcgtag tatcgggatg tttggggtcg cccattttca atttagcgca ttcggcaaga
     1141 aacagcgtga taaaacgatc ggctgcggct tcggttacga tgatgcgctt ggcggcgtta
     1201 cacgattgcc ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc acgctccaaa
     1261 tcagcatcgg gcatcacgat aaaggcgttg ctaccgccga gctccaacac ggttttctta
     1321 aggtttgcgc ccgcgtgtgc cgcaaggatg cgccccgtat acgttgaacc ggtaaacgcc
     1381 attgcatcgg tatcttcaac cgccttgagc gtgcccgcct catccagcca cacgcctgcc
     1441 agaggaatgc cgtctgaagc caaatcgaac agtgcctgac tgacgcgtgc cacgctgggc
     1501 gcgggtttga cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc
     1561 tgccagacgg gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcgaagcgc
     1621 acctgactca aactcgcctg cgtcgcgatg gttttgtggg caagcagttc gggggcaagg
     1681 cgggcgtaat agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcaag
     1741 cagcgtccga cttcctcaca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg
     1801 tcggcaaatt tttgcaggcg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg
     1861 ccgcgcattt tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg
     1921 cggtaaagcg tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag
     1981 ttgcgggaat atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat
     2041 catacccatg atacggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt
     2101 cccggcaacg attaaaatgg cataacaaat cgcactgacc accaaaccgg ccacgataat
     2161 cagcgcgatg tcgctgtacg ttttggctgc cgaagcgtaa ataatcacgg tcgaaatacc
     2221 gcccgggccg atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg
     2281 ggcggggcgc acctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc
     2341 gttgccgttc atcatcgaaa tggcaatcag caacaccaaa attccgccgc cgacctgaaa
     2401 cgaaccgacg ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc
     2461 aatcacggca aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct
     2521 gtgcccgttg gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa
     2581 aaaagccaca atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa
     2641 acccgtgcgg caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa
     2701 ttaaaaattc aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca
     2761 aggatgcttt tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga
     2821 ctgcacctga ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact
     2881 atggtcgaac agatgcaaac catcaacaca gacggcatcg aaccgatggc gcacccgcac
     2941 gaggccgccc tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat
     3001 caggcgggtg ctccggaagt acgcaaccgt ctgtacatcg taccgcaagt tatcgaagaa
     3061 taatccgaat atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa
     3121 gatttatgac ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt
     3181 ccgccgtcga actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca
     3241 acggctatat caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac
     3301 gtatcgcgca gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt
     3361 tctgccaaac cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc
     3421 cctacaccgc caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca
     3481 ccaatatgga tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca
     3541 aaaacccatg gaatcttgag cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg
     3601 ttgccgcgcg cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccagc
     3661 ctgcatcgca ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg
     3721 gtatggtcgc ctacgcctcc agcttcgacc aggccggtcc gatggcgcaa accgccgaag
     3781 actgcgcgat tctgttgaac gcgatggcag gtttcgaccc caaagactcc accagcctcg
     3841 aacgcgaaaa agaagactac acccgcgatt tgaaccaacc gctcaaaggc gtgaaaatcg
     3901 gcctgcccaa agaatatttc ggcgaaggca acagcgccga tgttcagacg gcattgcaaa
     3961 atactatcaa cctcctcaaa acccaaggcg cggaattggt cgaagtttcc ctgccgcaaa
     4021 ccaagctgtc catccccgcc tactacgtcc tcgcctccgc agaagccagt accaaccttt
     4081 cacgttacga cggcgtacgt tacggacacc gtgccgccca attcggcgat ttggaagaaa
     4141 tgtacggcaa aacccgcgcc gaaggtttcg gcagcgaagt taaacgccgc atcatgatcg
     4201 gcacttatgt actgtcacac ggctactacg atgcctacta tctcaaagcc caaaaactgc
     4261 gccgcctcgt tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc
     4321 cgaccgcacc cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact
     4381 tgagcgatat ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg
     4441 caggcttcag cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg
     4501 aagccaaaat cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac
     4561 gacccgaatg aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta
     4621 tgcgccttta tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa
     4681 agaaatttac gccttcgagc agtttgtcaa cgcatcccct atccgtcagg ggctgttcct
     4741 ccactgcccg caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg
     4801 caaacgccgt ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac
     4861 agacatcctg caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga
     4921 cggcatcagc ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt
     4981 atctttgcgc gacgaagcag gcaaccggct gtatatggcg accttcgcct ttgtcggcac
     5041 acacctgctg acagcctccg tacaagggcc ggcgggtgaa gaagccaaag acaccgtccg
     5101 ccgcataacc aaacaactcc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca
     5161 atatttcgcc gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt
     5221 caaactgcgc tggaaactta aaaagcgtgt caaaatgaat tacgacgcat tctggcagga
     5281 atacggcgca agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa
     5341 agaccttacc gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct
     5401 ggacaatatg gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga
     5461 cggcatccaa acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga
     5521 tattttagag aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt
     5581 ccaattgaac accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc
     5641 caacgcgcac gccggcgtag tggaatgtgc tttgccgggc gtactgccgg taatgaaccg
     5701 agaagtcgtt gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa
     5761 aaacgtgttc gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag
     5821 ccagttggac ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt
     5881 gaaaaccatc aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtacatga
     5941 aggcttgaac ggcgcgaccg gtatcgattt gaaccgcgcc ggcacgcctt tattggaagt
     6001 cgtatccgaa cccgaaatgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca
     6061 cagcttggta acctggctga acatttgcga cggcaatatg gcggaaggtt cgttccgcgt
     6121 cgatgccaac gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat
     6181 taaaaacctc aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca
     6241 aatcgagatt ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga
     6301 aaaaggcgaa acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc
     6361 cgaccccgat ttgctgcccg tcatcatttc agatgcccaa atgcaaaaag ccaaagcaga
     6421 aatgcccgag ctgccgaaag aaatggcagc gcgtttcgtg gcggactacg gcgtgtccga
     6481 atacgacgcg cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc
     6541 caaagagagc ggacaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc
     6601 actgaacaaa gaaggcatgg aacttgccga cagcccgatt accgctccgc gcctcgccgc
     6661 gctggtaggc aaaatcgccg acggcacatt aagcagcaaa ttagcgaaaa aagcctttga
     6721 agccatgtgg gcagaacccg aagccactat tgccgaaatc atcgaaaaac acggtttgca
     6781 gcaaatgacc gataccggcg cgattgaagc catggtggac gaagtgctgg caaacaacgc
     6841 caaagccgtg gaacagttca aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca
     6901 agtgatgaag gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc
     6961 caaacttgcc tgacaagcaa gatgccgtct gaaacccaaa tccgacgttc agacggcatt
     7021 ttcacagtct gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta
     7081 ccctgcccga ccaaaccacc tttgccgcca acgacggcga aaccgttttg gccgctgccg
     7141 cccgacaaaa cctcaacctg ccccattcct gcaaaagcgg tgtctgcgga caatgcaaag
     7201 ccgaactggt cagcggcgat attcaaatgg acggacactc ggaacaggct ttatccgaag
     7261 cagaaaaagc gcaaggcaag attttgatgt gccgcaccac cgcgcaaagc gatatcagca
     7321 tcaacatccc cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg
     7381 aaagtattat tttcaaacac gatgtcgccc tcctgaaact tgccctgccc aaagccccgc
     7441 cgtttgcctt ctacgccggg caatacattg atttactgct gccgggcaac gtcagccgca
     7501 gctactccat cgccaatccg cccgaccaag aaggcatttt ggaactacac atccgcaggc
     7561 gcaaaaacgg tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag
     7621 gcatcgtccg cgttaaaggt ccgctcggtt cgtttaccct gcaggaagat agcggcaaac
     7681 ccgtcatcct gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc
     7741 ttatccgcca aaacagcagc cgcgccgtcc atttctactg gggcgcgcgt catcaagatg
     7801 atttgtatgc cctcgaagaa gcacaagggt tggcatgccg tctgaaaaac gcctgcttca
     7861 cccccgtatt gtcccgcccc ggagagggct ggcagggaag aaaagggcac gtacaagaca
     7921 tcgcggcaca agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg
     7981 ccatgaccga acaagcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt
     8041 ttttctccga cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg
     8101 ctttccgttc ggaacacaaa aaacttcccg tccgtatttt ccccataaaa aatgccgtct
     8161 gaagcccgat tccggttttc agacggcata tgttttttcc tgttcaaggc gacagccgct
     8221 cgcgtatcca gccaccatcc agcaaacggt attggatgcg gtcgtgcagc ctgctcggcc
     8281 tgccctgcca aaactcaatc aaatcgggaa tcacaatata gccgccccaa tgcggcggac
     8341 gcggcacgtg caaaggatgt ttgagtccga ccgccgccgc ctttgccacc aataccgcct
     8401 tgttcggaat cacctcgctc tgcgcacttg cccacgcacc caaacggctc tgatacgggc
     8461 ggctctcaaa atattcgtcc gacaacttct ccgccagcct ttcaacacgc ccttccacgc
     8521 gcacctgacg ctccagctcc ggccaaaaaa acgtcatcgc cgcaaatgga tgagcatcca
     8581 gcgaacgtcc cttacggctg tgataattcg taaagaaaac aaaaccttca gaattaactt
     8641 ccttcagcag caccatacgg ctgttgggtc tgccgcgtcc gtcgaccgcc gccacattaa
     8701 ccgccgtcgg ctcgttgacc tgtgcgcgta ccgcctcgtc caaccaccgc tcgaactgct
     8761 cgatcggatt gtcggcgcaa tcgccttccg acaattcccg tttactgtaa tcttcccgaa
     8821 tattgtgcaa atccatttac cgctcctctt gtcatttgaa agattctacc cccgcacgca
     8881 aaccgatttc aaccgttgca caaactttgc ctcgcttctc caaacctcag gacacaacga
     8941 tttcatccgc aaaaccgccg cataaggtac aatatcgaac cgtccgaccg agggcggcat
     9001 tttatcaacc cgtcctgccg cacacgccgc agaagaaccg ccttatcagg cgagttagga
     9061 aaaatgatgt ccaaacagcc caccagcaaa cgccaatggc gcgacggcgt agccccgtct
     9121 gccaagaaaa ccgccaaacc tttcaaaagc aaagcccgtc ccaaagacga aacggacaaa
     9181 accgcatccc aaccttacgg acaaaaagct tcagacggca tcaaacctca aaacctcccc
     9241 aaacagcgcg ccgccaaagc caaaaaactc gtcgtccgca atcccaacca aaaaattatg
     9301 gaacacgcgc gcgatttaaa agaacgccgc agcgacctat cgcgcatgga acccgaacgc
     9361 ctgcaaaaag tgcttgccgc gtccggcgtc ggctcgcgcc gcgaaatgga agaatggatc
     9421 aacaacggct gggtaacggt caacggcaaa accgcgcaac tgggcgacaa agtcaccccc
     9481 gacgaccacg ttaccgtcaa aggcagcatc atcaaactca aatgggccga ccgcctgccg
     9541 cgcatcatcc tatattacaa acaagaaggc gaaatcgttt cccgcgacga cccgcaaggc
     9601 cgcgtcagca tattcgaccg cctgccgcag gccgccagca gccgctgggt cgccatcgga
     9661 cgcttggaca tcaacaccag cggacttctg attctcacca cctccggcga actcgtccaa
     9721 cgtttcgccc accccagctt cgaagtcgaa cgcgaatacg ccgtgcgcgt cttgggcggg
     9781 ctgaccggcg aacaaatgcg cgtcctcacc gaagaaggcg tgatgctcga agacggcttg
     9841 gcaaaagtcg aacgcatccg cgaacaaggc ggcgaaggcg cgaacaaatg gtacaacgtc
     9901 gtgattaaag aaggccgcaa ccgcgaagtg cgccgcattt ttgaaagcca agggctcacc
     9961 gtcagccgcc tcgtgcgcgt cggcttcggt cccatcggac tgcccaaccg cctcaaacgc
    10021 gggcagttct acgaactcaa ccccgccgaa gtcgccaaca tcatcaaatg ggcggacatg
    10081 ctgctgccgg gcgaacgccg ccgcaaaaaa gcctaaaccc gccaaaacac aaaaatgccg
    10141 tctgaaacat ctgctgtttc agacggcatt ttattcgggc gttttcagga gaaaaggtcg
    10201 agtgctttga caaagaccat caccacgccg taggcgagcg gtatgacggc caatgcccaa
    10261 cgccaccaga ccgatatgcc gccgccggaa actttttcgc ccacaaggta agcgtcggat
    10321 atggcggttt cgtcatcggg gttgccgctg tgtgcggcgg ttttgatgtc tttttcgtgg
    10381 tgtttttcgt gtacggattt gacggcgagg ttgcacaaca aaccgataat cagcagaccc
    10441 gccatgatgt acatggttac gctgtatgcc tgtgccgccg gtatgccgct gtcgatttgg
    10501 ctttggcgta tgtaattgac cagtaccggg ccgatgacgg cggcggttga ccaggccagc
    10561 aggatgcgcc cgtgaatcgc gccgacctga taggtgccga acaggtcttt caggtaggcg
    10621 ggaatggcgg caaatccgcc gccgtacatg gaaataatca cgcaaaagcc gatgatgaac
    10681 agggctttgc tgccgccctc gccgatggag ggaacggcga aatacagcag cgaaccgagt
    10741 acgaagaaga tggtgtaggt gtttttgcgt ccgattttgt cggaaacgct cgaccacaaa
    10801 aagcgtccgc ccatgttaaa caggctcagg aggctgacga agcctgccgc cgcacctgcg
    10861 ccgactgctg cctgcctgcc tatggaggtt tcggaaaaga gttcctgaat catcacggat
    10921 gcctgaccca atacgccgat gccggcagtt acgtttaggc acaataccca gaacaacagc
    10981 caaaactgcg gcgttttcat ggcttgggac acgttgacat gattgctgct gaccagcttg
    11041 ttttgcgttt tcggcgcgat atagccttca ggtttccagc cgtcggcagg tacgcagatg
    11101 gtaaacgcgc cgaacatcat cagtgcgagg taaagcagtc ccaatacggc aaaggtttca
    11161 acaaccccga ccgaagcggc gtttgaaaag gcgttcatca gtgatacgga aagcggtgag
    11221 gccagcattg cgccgccgcc gaaacccata attgccaaac cggtcgccat acccggcttg
    11281 tcgggaaacc atttcatcag tgtggaaacc ggcccgatgt agcctaagcc taagcctacg
    11341 ccaccgatga cgccgttgcc caaataaagc aggaagaggt tgtgcgtact cacgccgaat
    11401 gcggatacga agaagcccag gctgaaacag caggcggcgg caaatatggc tttgcgcggc
    11461 cctacccgtt ccatccatgt gccgaacagg gcggccgacg cgcccagcat cgcgagtgcg
    11521 atactgaaaa tccaacctac ggtcgtcagc ttccaatctc cggccgccga ttcggttatg
    11581 ccgataagtt tggtcagcgg cgcgttgaat acggaatagg cgtaaatctg cccgatggca
    11641 aggtgtaccg ccaatgctgc gggcggtacg agccaacggt tgaaacccgg cttggcaatg
    11701 cttgcctcac ggtctaaaaa cttcataaca tcctctttct gtcagttgaa aaataaaatt
    11761 tcatttgctc aatggaaact tattgaaaat tataaaaaaa tatcgggtcg ggtttttatc
    11821 cgccccaaga tgcgccgtct gaaacatttc gggtgtacgg aaaggtttct gttttttccg
    11881 acaaattcct gcggcttttc gcttccggat tcccgctttt gcgggaatga cgaattaaag
    11941 attatcttaa ggtcaaggga ctggattccc gctttcgcgg gaatgacggc ggagggggag
    12001 cggtttttcc gattgggttt aaatgcaatc gaacaaatcc tgctgccctt gttctttgct
    12061 tacgcgcacg tcggtttcgc cgtcggcgaa ggtaatgtgc agcttctgcc cctgcttcaa
    12121 aacatcggcg ttgcggatga cttgtccgcg tgtatctttg acgacggaaa agccgcgttc
    12181 caaaatgtgc tgcggcgaaa cggcttcgag caatgcggct tgggcggtca ggctttggcg
    12241 gcggtgggta agcagttggc tgaaggcggc gggcaatgcc gtctgaaggc ggacgatgtt
    12301 gtttttgcaa acggaaacat caggacggta atgttgcagg gcttgggttt ggcgttcaaa
    12361 acgggcggtg tgggcacgga ggttttgctt catagagtaa gacagcgttt gcgccagctt
    12421 gccgattgaa gcgcgctgct catcaagttt ttggcgcgga tgacggattt gccgcgcaag
    12481 ccagtcgagt ttttggctgg catcgaaata gcgttgttcc aaaacggttt tcagacggcc
    12541 ttgcgcctgg gcgagacgat gtagcgattc ttggcggttg gggctgacca gttccgccgc
    12601 gccggtcggc gtgggcgcgc gcacgtcggc gacaaaatcg gcaagcgtga aatcggtttc
    12661 gtgtcccacg ccactgacga cgggaaccgt gcaggcttcg attgcgcgta cgaccggttc
    12721 ttcgttaaac gcccacaaat cttcaatgct gccgccgccg cgacagacaa tcaacacgtc
    12781 gcattcggcg cgttgcgatg cggttttaat cgcttgggca atctgaaact cgctgcctgt
    12841 gccttgaaca ggtgtcggat aaacgataac gggaatttcg ggcgcgcggc gtttcaaggt
    12901 ggttacaaca tcgcgcaatg ccgccgccgc caaactggtt acgatgccga tacattgagg
    12961 gcggacgggc aacggttttt tgcgttcctc cgcaaacgcg ccttccgcct gcaactgcgc
    13021 cttcaaccgc tcataggctt cgtaaagctg ccccaaacct ttgagccgca cttcgttcac
    13081 ggtaatctga aattcgcccc gcgtttcata aatactgatt ttgcccgcca cttcgatatg
    13141 gtcgccttct ttcaaaggtt tcgccaaacg cgccgccgca cccttgaaca tcgcgcaacg
    13201 cacctgcgcg cggctgtctt tgagcgagaa ataataatgc ccgctggcgg cacgggtcag
    13261 gttggacact tcgcccgcaa tccacagacc ggcaaggtgg ttttccaaaa tacttttggc
    13321 aaatgcgttc aactcggaaa cggacaacac gtcagaatgg aaaaaatcag acatcgaatc
    13381 aatcaaatag taaaaaatat gaatatgttt tgaagcccaa ggcggcatag agccgcctaa
    13441 attgtcaaca atattataac acgcgccatc ttgccgcccg actttgcccg tatgactttt
    13501 ttaagcgggg aatgggaaaa atattcatca acctgcctgc aatctattca aattgaaccg
    13561 ccgacaagct atgatgcgga tattttcgac aggaggaacc atggatacgc aggcagttat
    13621 cacacatatc gcccgatggt tggacgaata cgccactcag gcaaatgcaa aaggattcgt
    13681 cataggcgtt tccggcggca tcgattccgc cgtcgtctcc acactcgccg cccgcaccgg
    13741 ccgccccacg ctgcttctgg atatgccgat acgccaacac cccggccagc ttgagcgggc
    13801 aaggctgcac atccgcaatc tgcaacggca atatgccaat gtaagcgcgc aaacggccga
    13861 tttgaccgac accttccaga cctttgaaca aaccgtcggc attcatcaga cggcatttga
    13921 caatcagccg ctttccctcg ccaacgccaa aagccgcctg cgtatgctga ccctgtacta
    13981 ctacgggcag atacacggac tgctggttac ggggacaggt aataagattg aagatttcgg
    14041 cgtgggattt tttaccaaat acggcgacgg cggcgtagac atcagcccga ttgccgacct
    14101 gaccaaaacg caggtttacc ggcttgccga agcattgggc gtggacgagg cgattcaaaa
    14161 agccccgccg accgacggcc tgtgggatac ggaacgcacc gacgaagaac agatgggcgc
    14221 aagctatccc gaactggagt gggcaatggg cgtgtacggc acgcgcaaac ccgaagattt
    14281 tgaagggcgg cagcgcgaag ttctggaaat ctatacgcgg ctgcaccgcg cgatgcagca
    14341 caaaatcaac ccgattcccg tgtgccgcat tccgcccgaa ttgttgggct gaaacgtgga
    14401 aatgccgtct gaagcggaaa aaccgtattt cagacggcat ggaaatatcc ggctcccgtt
    14461 ccttaagaat cgagtacgcg ggcaaacaga atatcgtttt ccaaatgaat gtggtcgttc
    14521 aaatcctcca ccatttcttt ggccaaagcg taaaggcgcg tccagcttcc gcaagcccct
    14581 tctggcggtt ggaaattgtc ggtcagctct ttgagccgtg cgatggcgcg gtcgtgttct
    14641 tcgtgttcgt gcatcatcac gctgatgggc attgccgcac cgcgtccgac accctgatta
    14701 atcatcggaa acagcatcct ttcctctttc atcatatgca tcagcagttc gttctgcata
    14761 tcggcaagca gcctggcaat ttccgccgga aaggtatcgg catggacttg gaccactttc
    14821 tgcgccagcg gcaccaattc ttcaaattgt gcgcggtgga cattgtggta gcgttgcagg
    14881 atataatcga cggttgcgcc aaagggggct gtttcccaaa cggaaaaatc agtcattgca
    14941 gtgttccttt tatagggttt cgggtttggt tttgaacatt catactttaa gaatctattt
    15001 aaacggagca tacaccgccc gcgcgtttct gtacagcttc aaaaacattc cttacacttt
    15061 gataataaaa gtaattttca aaaataaaat actgtccgaa ccgtttttta gaatttgcaa
    15121 aggcgattgg ggcggtacag aaaaactatt atcccgcccg cccacttgaa atttttatgc
    15181 ccaagcccta tcctgcacgc tatcgtgcca atcccaaccg aaaaggaaaa ataatgagca
    15241 gcgaattgat tatacacacc agcgatgccg cttttgaaaa agacgtttta aattcagata
    15301 cccccgtcct gctggacttt tgggctccgt ggtgcggccc ctgcaaaatg attgccccga
    15361 ttttggacga catcgccgcc gaatttgaag gccgtctgaa agtggtcaaa atcaacatcg
    15421 acgacaacga agccaccccg tcccgtttcg gcgtgcgcgg cattccgacc ctgatggtgt
    15481 tcaaaaacgg cgaagtcgtg gctaccaaag tcggcgcatt ggcaaaaggt cagctgaccg
    15541 cctttgtcga agcctccatc gcctgataaa gcgcaatcga aaaagccgcc ggaagattcc
    15601 ggcggctttt tcgcaccctt aagatttgtg gcggatttcc cagcacctat ggattttttt
    15661 gttgcggaaa tcttcgggaa cggattgttt ggaaatgtct ttgacggcgt attgttccga
    15721 taccaagtcg tctaagacga agctgctcag gttgttggaa aagtacaaaa tgccgtctga
    15781 agcgagcagc ttcaccgcgc cgtcaatcag ctttttgtgg tcgcgctgga tgtcgagaat
    15841 gtcgagcatc tttttgctgt tggagaagct gggcgggtcc atcacaatga ggtcgaaccg
    15901 cctgccttcc ccatatgccg tctgaagata ttgaaacacg tcggcgcgga cgattttgtg
    15961 tcgttccgta tcgatgccgt tcaattcaaa attgcgtttc gcccaatcaa gatatgtgtt
    16021 ggacaaatcg acggtttcgc tggatgccgc gccgccggtg gcggcataga cggtgaagct
    16081 gccggtgtag gaaaacaggt ttaaaaaacg tttgcccgcc gccgtttcgc cgactttttt
    16141 gcgcgtgttt cgatgatcca aaaaaagccc cgtatccaaa tatttatcaa ggttgaccca
    16201 aaacttgcgg ccgttttcgg tgatgacgaa atcgtcgccc gccttgccgg ttttctcgta
    16261 ctgctgcaaa cctttttggc gttcgcggcg tttgaggcgg atttgttcgg gcgcaaaacc
    16321 ggtaacgaaa gcgacggctt ccaagacttc ggcaagccac gcttcgtatt cttcgggccg
    16381 catcagccag ccggtatcgt attcctgaag gtggattcga tcgccgtaaa catcggcggc
    16441 aaaggggaat tgggggatgt cgcggtcgta aatgcgccag gcttcgatgc cgttgcgttt
    16501 cacccatttc ataaggtgtt tgatgttttt gcccaagcgg ttggcaaacg gtgtgatgtc
    16561 ggtcattggt ttcaggcgga ataaagtgga aaacggcaat tttactgtaa ttaacgcccg
    16621 attgcttgac cgtttcgggc aaaccctata ccatccgtcg cttatcttgt catacgaagc
    16681 catcgccttc caacctaaac cgcccttacg ggcgcgtttc ttctgttgct ttgattttgc
    16741 aaagcatatc tgtgcaggtt gccgtcgatg taaaccacaa gcaagccgct tgcgacaacc
    16801 ctgtaacttc acattccccg tatcgttacc cttccctgct tcaggccgtc tgaacctttc
    16861 ggacgcgggc gttgttgtct tccaaggata gccatgtcta ttaaatttgc cgatttgaac
    16921 cttgataaaa acattttgtc cgccgtcagc agcgagggtt acgaaagccc gaccccgatt
    16981 caggcgcaag ccattccgtt tgctttggaa ggccgcgaca tcatggcttc ggcgcaaacc
    17041 ggctccggca aaaccgccgc ctttctgtta ccgactttgc aaaaactgac caaacgcagc
    17101 gaaaaaccgg gcaaaggccc gcgtgctttg gtgttgaccc cgacccgcga actggcggct
    17161 caagtcgaga aaaacgcgct ggcgtatgcc aaaaatatgc gttggttccg caccgtcagc
    17221 atcgtcggcg gcgcgtcttt cggctaccaa acccgtgccc tgagcaaacc ggtcgatctg
    17281 attgtcgcca cgccgggccg tctgatggac ctgatgcaaa gcggcaaagt tgattttgaa
    17341 cgtttggaag tgctgatttt ggacgaagcc gaccgtatgt tggatatggg ctttatcgac
    17401 gacatcgaaa ccatcgtgga agcaacgccg agcgaccgtc agactttgtt gttctccgcc
    17461 acttgggacg gcgcggtcgg caaactggcg cgcaaactga ccaaagaccc tgaaatcatc
    17521 gaagtcgaac gcgtggacga tcaaggcaaa atcgaagaac aactgctgta ctgcgacgat
    17581 atgcgccaca aaaaccgcct gctcgatcat atcttgcgcg atgccaatat cgatcaatgc
    17641 gtgattttca cgtccaccaa agccatgacc gaagtcattg cggatgaact gtacgaaaaa
    17701 ggtttcgccg caaactgcct gcacggcgat atgccgcaag gctggcgcaa ccgcacgctg
    17761 atggatttgc gtaaaggccg ctgcaaaatt ttggttgcca ccgatgttgc cgcacgcggt
    17821 atcgacgtac cgaccattac ccacgttatc aactacgacc tgccgaaaca ggcggaagac
    17881 tacgtccacc gcatcgggcg caccggccgc gcaggccgca cgggtattgc gattacgttt
    17941 gccgaagtga acgaatacgt caaagtccac aaaatcgaaa aatacattaa ccgaaaactg
    18001 cccgaactga ccatcgaagg catggaaccg acccgcaaac gcaaatccgc aggcggcaag
    18061 ccgaaaggca aaggcggctg gggcgatcgt aaatccggcg gttggcgcgg cgatcataaa
    18121 ccgagcaaag aaggcttcgg cggcaaaacg cgcggcgaag gtttcaagaa agaaggcttt
    18181 aagagagacg gtttcaaaaa aaccggcgaa ggcttcaaag gcaaacgcaa agccggcgat
    18241 tcttttgcag gcaaaggcga acgccgttac aaagaccgct aagccccaac ctgccgcata
    18301 aaccaatgcc gtctgaaacc gatttcgagt ttcagacggc atttttgcaa tgtttcagca
    18361 ccgcccggct ttgataccca aaggattaat c
//
LOCUS       DAXHMX010000023        17721 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHMX010000023 DAXHMX010000000
VERSION     DAXHMX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.023"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..386
                     /locus_tag="ACFM4S_000601"
     CDS             <1..386
                     /locus_tag="ACFM4S_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6050334.1"
                     /translation="SLGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVETETK
                     IIFTTPTKGAKVGGTILDKSMSKPPYHESNSISSLGLGVIAGVGFDITPKLTLDTGYR
                     YHNWGRLENTRFKTHEVSLGMRYRF"
     gene            complement(360..569)
                     /locus_tag="ACFM4S_000602"
     CDS             complement(360..569)
                     /locus_tag="ACFM4S_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050335.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKCA
                     GKGLMPSEGSDGIGIGESEAVAHAQ"
     gene            complement(587..1144)
                     /locus_tag="ACFM4S_000603"
     CDS             complement(587..1144)
                     /locus_tag="ACFM4S_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6050336.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1220..2854)
                     /locus_tag="ACFM4S_000604"
     CDS             complement(1220..2854)
                     /locus_tag="ACFM4S_000604"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6050337.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPVGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2924..3257)
                     /locus_tag="ACFM4S_000605"
                     /pseudo
     CDS             complement(2924..3257)
                     /locus_tag="ACFM4S_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3257..4363)
                     /gene="serC"
                     /locus_tag="ACFM4S_000606"
     CDS             complement(3257..4363)
                     /gene="serC"
                     /locus_tag="ACFM4S_000606"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6050338.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPCLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSDGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4728..5159
                     /gene="rimP"
                     /locus_tag="ACFM4S_000607"
     CDS             4728..5159
                     /gene="rimP"
                     /locus_tag="ACFM4S_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6050339.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            5187..6689
                     /gene="nusA"
                     /locus_tag="ACFM4S_000608"
     CDS             5187..6689
                     /gene="nusA"
                     /locus_tag="ACFM4S_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6050340.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVDENQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6701..9589
                     /gene="infB"
                     /locus_tag="ACFM4S_000609"
     CDS             6701..9589
                     /gene="infB"
                     /locus_tag="ACFM4S_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6050341.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQITGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHARLIRNNVVIHTGE
                     LSSLKRYKDDVKEVRMGFECGLMIKGFNEIMEGDQLECFDIVEVARTL"
     gene            complement(9800..11194)
                     /locus_tag="ACFM4S_000610"
     CDS             complement(9800..11194)
                     /locus_tag="ACFM4S_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HFC6050342.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDATRLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11256..12605)
                     /locus_tag="ACFM4S_000611"
     CDS             complement(11256..12605)
                     /locus_tag="ACFM4S_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6050343.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIILNDAPKWAVMLETEWQDGA
                     WFEGRLAQEWLDKGVAANREQVAALEAELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKTNDRT"
     gene            12748..13374
                     /locus_tag="ACFM4S_000612"
     CDS             12748..13374
                     /locus_tag="ACFM4S_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6050344.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13438..14856)
                     /locus_tag="ACFM4S_000613"
     CDS             complement(13438..14856)
                     /locus_tag="ACFM4S_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6050345.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            15183..15911
                     /locus_tag="ACFM4S_000614"
     CDS             15183..15911
                     /locus_tag="ACFM4S_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6050346.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15970..16458)
                     /locus_tag="ACFM4S_000615"
     CDS             complement(15970..16458)
                     /locus_tag="ACFM4S_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6050347.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17216..17680)
                     /locus_tag="ACFM4S_000616"
     CDS             complement(17216..17680)
                     /locus_tag="ACFM4S_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6050348.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4651 a         4942 c         4465 g         3663 t
ORIGIN      
        1 cttctctcgg cttatccgcc atttacgatt tcaaactcaa cgataaattc gataaattca
       61 aaccctatat cggtgcgcgc gtcgcctacg gacacgttaa acatcaggtt cattcggtgg
      121 aaacagaaac caagattatt ttcactacac caacgaaagg cgctaaagtt ggaggcacga
      181 tcctagacaa atcgatgagc aaacctccct atcacgaaag caacagcatc agcagcttgg
      241 gtcttggcgt tatcgccggt gtcggtttcg acatcacgcc caagctgacc ttggacaccg
      301 gataccgtta ccacaactgg ggacgcttgg aaaacacccg cttcaaaacc cacgaagtct
      361 cattgggcat gcgctaccgc ttctgattcc ccgataccga tgccgtctga accttcagac
      421 ggcatgagac ctttgcctgc gcacttggtg cgctggtcgc ctccgaacat ggcgcgacac
      481 ccgacatttc cgccgaacgc atcgggcgtt tcatgaatcc ggtttaaaac gcatggaaaa
      541 atgccgtctg aaagcctttc agacggcatt gtgcttgaga ttccgtttac caatgactga
      601 caaacgcttc caaatcggta ttcttgggct tatgcacttc ctctgtcggc gtgccgacca
      661 tcatcagccc gatgatttta tccttatccg cacatccgaa agcctcacgc aacagggggc
      721 tgttgaccca catccccgta atccagacat tgtcgaaacc ttgcgccgtt gccgccagtt
      781 gcagcgcata cgccgcacaa cccgccgtca gcatctgttc ccattccggt ttcggcttag
      841 gcacatcgcg gttcggcgca aacgttaccc cgataaccat cggcgccata ttgcccactt
      901 tttccgcctt tttcatcgca tcgtcgccga aattcaattc ggcaaccgtt tgcttcaaca
      961 catcgcgaaa acgttgcaat cctacctcgc cttgaatcac ggtaaaacgg aaggggcgca
     1021 tattgccgtg atcgggaact tgggttgccg cctgaaatat ttgttccaac tccgccgcat
     1081 cgggggcggg gggcttcagc tttttggaag atcggcggtt cgtcaataat tttaaagcat
     1141 ccatatcgtt atactccggt catcggtcgg gtgttcggac acatatgccg tccgaaggct
     1201 tcagacggca tatccggcat cagcgcggac ggcggcaggc tgccaatata tccatttcct
     1261 tccgataggt ttggctattg gaaatgtcca tcagccccaa taccgtgctg aaatagtggt
     1321 cgtgcgaata ttcgttttcc gccgcttttt gtttgaggca ttggaaatct atgcccccgt
     1381 gttggcggaa ggctttggaa aaccacataa ccatcgggat atgcgtctgc ccggaaggcg
     1441 cgatggcgta aggcgcggcg tgcaggtaca tcccgttttc gcccaaactt tcgccgtggt
     1501 cggaaacata atgcaccacg ctttccaaat cgtcgcggtt ttcaagtttg cggataacct
     1561 tgtcgataaa ctggtccaca tacaaaaccg tattgtcgta agtgttgacc agcgtggcgc
     1621 gggtgcattt gttgatttcg ttggtgtcgc aggtcggcgt gaatttgcgt tcggcttcgg
     1681 tatagcgttc gtaatacgtc ggcccgtggc tgccgatggt atgcaggatt aaaaccgcgt
     1741 ctttatcgtt tttgttgagg acttcgtcga acttggtcag caggatattg tcgaggcact
     1801 cgccgttgcg gcagtattcg ggcaggttga gcgaggtaac gtcggtattc ggcactttgc
     1861 cgcacacgcc cttgcagccg gaatcgtttt ccaaccaagt aacttccacg ccggcgcgct
     1921 gcacgatgtc cagcaggttg tcttggtgtt cggctttgat ttcgtcataa tccgtgcggt
     1981 cgaaggttga gaacatacac ggcagggagt gcgcggtcga tgtgccgcag cttctgacct
     2041 gcgggaaatt gacaatttca tcgccgcgcg cggcaagcag cggcgtagtt tggcggctgt
     2101 aaccgttcaa accccagttg gcggcacgcg tggtctcgcc cacgaccagc accacgaaac
     2161 ggcgcaggct gccgaccggg cggttttgca cgaccgccat atccaattgc gtataaggaa
     2221 tattggaacg cttccaatct ttgtatttcg acacgcccgc gccgatgaaa ttagacggca
     2281 caatcagatg ggttactgat ttattgttgc ggaaaaacga ggcgtaatcc tgatattgca
     2341 acattgcgat gcccaacgcg cacaaaaagg aaacggcggc aagcacaagg cgcgtcaaaa
     2401 gctccttata ccaaacgcgg tatttaacct tgacggcgat atacgccagc gcgggcaata
     2461 cgcccaaaca tacaatccac agcacatagc ccggcgtaat caggcgcgcg ctttcggcag
     2521 ccgtagtttg caagacatta ttcaacatcg acttgttgaa atagatattg aaaaatattt
     2581 cttggtaaga caccgccgca ctgataacca atatcaacgg aatcaatacc ttatgcacga
     2641 aaggcaaggc aatgacgtga aaaacgaaat tacttaaaaa aaacagcacc accggcatcg
     2701 tatagaggaa gatatccgcc ccggtgccgt taaaaggatg aagctcgaca actttggcaa
     2761 aaaaggcgta attcaatacc agcgaggaat acagggaaag gaaggcaatc agcgcggaag
     2821 agccgagctt cggcctcagg ttcggtttta tcatttggaa tgtgtcggat aagggttgga
     2881 aaaggcatcc ggcatttgga atccggatta ttgaaaaaga ttcttaatta taaggcaacg
     2941 gagcaaagca gggcaagaaa acggcggctg tgcgggggat tccgcccgcc attcaaacgt
     3001 ccggcagaca taaaaacatc gtaagcaaga ttcgaaccgg tctgcaaccg cccctgccag
     3061 aaaaacgggc aaagcatttc atattggaaa aacccggccg cgccgccgac gggacagtcc
     3121 ggcacaaaca gcatcacgcc cggattgaaa aggacggata gccgccggca gcacaatctt
     3181 accacctcca aaacgccgcc ggagaacgcc gaaacagccg atgccgtctg aagccgcttc
     3241 agacaacatc gggacatcaa ccgtaacgcc gttggaaatc gcgcataaaa tctgccaaag
     3301 cccgcacgcc ttcaagcggc atcgcattat aaatgctggc acgcataccg ccgacggttt
     3361 tatagccctt aagcaggcac aagccctgca attcggcttc cagcacaaaa cggcggtcaa
     3421 gctcctcatc ccccgtttgg aacacgacat tcattttaga acgcgcattc ggacggatac
     3481 ggttgatata aaaaccatcg ctgccgtcta tcgtctcata caaggtttgc gccttcagcc
     3541 gattgaccgc ttcaattttt ttcacaccgc cctgcgcctg tagccagcgg aacaccagcc
     3601 ccgacatata aatcgcgtaa gttgacggcg tgttgtacat accgtcgcgg ttgatgtgcg
     3661 aacggtagtt gaacacatcg ggaatatcgt tcggacaacg ctcgagcaaa tcctcacgca
     3721 caatcaccac cgtaactcct gccggcccga tgtttttctg tgcgcctgcg taaatcagtc
     3781 cgtagtcggc aacatcaaac tcgcgcgaca aaatctcgct ggacatatcg cacaccagcg
     3841 gcggcatgcc ttctgaaagg cacggcactt cacggtattg cagcccgttg accgtttcat
     3901 tgacggcaaa atggacaaac gccgaatcgg gtgcaacatc ccacgtttcc acaggtggca
     3961 ggtcgagata gtcgaactgc tcgccgccat gcgccgccaa acggatttcc gtatcggtca
     4021 aacggctcat ctgttcataa gcgatacggc tccagttgcc cgttaccacc gcgtcggcag
     4081 tgcggaaacc atgtgccaga ttcatggctg ccatattaaa ttgggttgtt gctccaccct
     4141 gcagaaacaa tatcttatag ttgtcaggca ctttcaaaag ctgcctcaaa tcctgttccg
     4201 catgatgcag gatgctcaaa aacatttccg aacggtggct cattgccatc acaggaaaac
     4261 ccgtaccgtt gtagtccaac atttcctgcc gcgccgtttc caacacggct tcgggcaata
     4321 cggcagggcc ggcggaaaaa ttgtaaatcg gataaagaga catgatgcag ccttgattct
     4381 gaacaataac ccgcccgatt ttaggctggc agcaggcttt gtgcaaggat gggaaatacc
     4441 tgtcctccgc cccgcttcat gccgcccgaa cacggaaaac aacatcaata tattgattta
     4501 caaacataaa aatcatgcta gcgacaaata gatacatttg ttttgtcaac aatattcacg
     4561 atttcccatt acaaacctcc cttacacccg cttttttccg tcccaaaaac acaaaataaa
     4621 tcaacacttt catttctccg caaaagcggt tataattacg ccgatttttc aactttgacg
     4681 aaaaatggcc acggggccat ttttttatta tgtacatagg gagcagcatg gatatccaaa
     4741 ccatcctcga aaaaaccctg cccggcctgg gctacgaact ggtcgatttc gaactgaccg
     4801 cgcaaggaac attgcgcgtg ttcatcgaca aagaaagcgg cattaccgtc gaagactgcg
     4861 caaccgtcag caaccatttg agccgcgtct tcatggttga agacatcgac tacaaaaacc
     4921 tggaaatttc cagccccgga ctcgaccgtc ccttgaaaaa agccgccgac ttcgtgcgct
     4981 ttgccggtca gaatgccaaa atcaaaaccc gcctgccgat agacggtcag aaaaacttta
     5041 tcggtaaaat cgaaggctgc gaaaacgata ccgttaccat atccttcgac ggcaaaaccg
     5101 tacaaatcga attgggcaac atcgacaaag cccgcctgcg ccccgaattc aaattctaaa
     5161 acacaacaat attggagatg ttcaaaatga gtcgtgaaat gttacagctg gcagaagcac
     5221 tggcaagcga aaaaaacgtt gatgcggaag tcgtcttcca agcactggaa ttcgccctgt
     5281 ctaccgccgc caagaaaaag gcagaccgcg aacacatgga tgtccgcgtc caaatcaacc
     5341 gcgataccgg cgaataccaa accttccgcc gctggctgat tgtcgccgat gaagactata
     5401 cctatcccga tgtcgaaaaa accatcgagg aaatccaaga ggaaattccc ggcaccgcca
     5461 tccaaatcgg cgaatactac gaagagcagc tgcccaacga aggcttcggc cgccaagccg
     5521 cgcaaaccgc caaacaaatc atcctgcaac gcatccgcga tgccgagcgc gagcagaatc
     5581 tgaacgagtt tctcgccgtc aaagaagaca tcgtgtccgg cacggtcaaa cgcgtcgaac
     5641 gccacggcat catcgtcgaa gtcgttgccg gcaaactgga cgcgctgatt ccgcgcgacc
     5701 aaatgattcc gcgcgaaaac ttccgcagcg gcgaccgcat ccgcgccctc ttcctgcgcg
     5761 tcgaagaaat cggcaacacc ggccgcaaac aagtcatcct gagccgtact tccggcgatt
     5821 tcctcgtcaa actgtacgcc aatgaagtac ctgaaattgc agacggcatg cttgaaatcc
     5881 gcgctgtcgc ccgcgacccg ggccagcgtg ccaaagtcgc cgtcaaagcc aacgaccagc
     5941 gcatcgatcc gcaaggcacc tgtatcggcg ttcgcggttc gcgtgtcaat gccgtcagca
     6001 acgaattgtc cggcgagcgc atcgacgttg tcctttggtc gcccgagcct gcccaattcg
     6061 tcatgagcgc gctctcaccc gccgaagtca gccgcatcgt gattgacgaa gacaaacacg
     6121 ccgttgacgt gattgttgat gaaaaccaac tggcgctagc catcggacgc ggcggtcaaa
     6181 acgtgcgcct tgcttccgac ctgaccggct ggcagctcaa catcatgact tccgccgagg
     6241 cagacgaacg caatgcggca gaagatgccg ccatccgccg cctgtttatg gatcacttga
     6301 acgtggacga agaaaccgcc gacgtactgg ttcaagaagg ttttgcaacc ttggaagaag
     6361 tcgcctatgt tcctgccgcc gaactgcttg ccattgaagg atttgacgaa gaaatcgtcg
     6421 atatgctccg caaccgcgcc cgcgatgcca tcctgaccat ggcgattgcc gccgaagaaa
     6481 aactgggcga agtgtccgac gatatgcgca acctcgaagg catagatgcc gatatgctcc
     6541 gcagccttgc cgaagcaggc attaccaccc gcgacgactt ggcagagctt gctgtggacg
     6601 aactgattga aatcaccggt gtaaacgaag aaaccgcaaa agccgtcatc ctgaccgcac
     6661 gcgaacactg gtttaccgaa gacaaataaa gggggtacag atgagtaaca caaccgtaga
     6721 acaatttgcc gccgagctga aacgccccgt cgaagacctg ttgaaacagt tgaaagaagc
     6781 cggcgtcagc aaaaacagcg gcagcgattc cctgacgctg gacgacaaac agcttctgaa
     6841 cgcctacctg accaagaaaa acggcagcaa cggcggcacc atcagcatcc gccgcaccaa
     6901 aaccgaagtc agcaccgttg acggcgtaaa agtcgaaaca cgcaaacgcg gacgcactgt
     6961 caacattcct tctgccgaag aattggcagc acaggtaaaa gccgcccaaa cccaagccgc
     7021 acctgtccgg ccggagcaga cggcagaaga cgcggcaaaa gcccgagccg aagctgccgc
     7081 acgtgcagaa gcccgtgcca aggcagaagc ggaagcggca aaactgaaag cggcaaaagc
     7141 aggcaacaaa gccaaacctg ccgcgcagaa acccaccgaa gcaaaagccg aaaccgcacc
     7201 tgttgcggcg gaaaccaaac ccgccgaaga aagcaaagcg gaaaaagccc aagccgacaa
     7261 aatgccgtct aaaaaacccg ccgagcccaa agaaaaagcc gccaagccga aacacgagcg
     7321 aaacggcaaa ggcaaagatg ccaaaaaacc ggcgaaacct gccgcacctg ccgtgccgca
     7381 acccgtggtc agcgcagaag aacaggcgca acgcgatgaa gaagcacgcc gtgccgccgc
     7441 acttcgcgcc caccaggaag ccctgttgaa agagaaacag gaacgtcagg cacgccgcga
     7501 agccatgaaa caacaggcag aacaacaggc aaaaaccgca caggaagcca aaaccggcag
     7561 acagcgtccc gccaaacctg ccgagaaacc gcaggcagcc gcgccggccg tcgaaaacaa
     7621 gcctgtcaat ccggcaaaag cgaaaaaaga agaccgccgc aaccgcgatg acgaaggtca
     7681 aggccgcaac gccaaaggca aaggcggaaa aggcgggcgc gaccgcaaca atgcacgcaa
     7741 cggcgacgac gagcgcgtac gcggcagcaa aaaaggcaaa aaactcaaac tcgagccgaa
     7801 tcaacacgcc ttccaagcac cgaccgaacc cgtcgttcat gaagttttgg ttcccgaaac
     7861 cattaccgtt gccgatttgg cgcacaaaat ggcggtcaaa ggcgtggaag tggtcaaagc
     7921 cctgatgaag atgggcatga tggttaccat caaccaatcc atcgaccaag acaccgccct
     7981 gattgtggtg gaagaactcg gccacatcgg caaacctgcc gcagccgacg accctgaagc
     8041 attcttggac gagggcgcgg aagcagtgga agccgaagca ttgccgcgtc cgcccgtcgt
     8101 taccgtgatg ggccacgtcg accacggcaa aacctcgctg ctggactaca tccgccgtac
     8161 caaagtggta cagggcgaag cgggcggcat tacgcagcac atcggcgcgt accacgttga
     8221 aacccctcgc ggcgtgatta ccttcttgga caccccgggc cacgaagcct ttaccgctat
     8281 gcgcgcacgc ggtgcgaaag caaccgacat cgtgattctt gtggtcgccg ccgacgacgg
     8341 cgtgatgccg caaaccatcg aagcgattgc ccacgccaaa gcggcaggcg taccgatggt
     8401 ggttgccgtc aacaaaatcg ataaagaagc cgccaaccca gagcgtatcc gccaagagct
     8461 gaccgcacac gaagtcgtgc ctgacgaatg gggcggcgat gtacagttta tcgacgtttc
     8521 cgctaaaaaa ggcctgaaca tcgatgcatt gctcgaggca gtcttgctcg aagccgaagt
     8581 tttggaactg accgcacctg tcgatgcgcc cgccaaaggc atcatcgtcg aggcgcgctt
     8641 ggacaaaggc cgcggcgcgg ttgccacatt gctggttcaa agcggcacgc tgaaaaaagg
     8701 cgatatgctg ctggccggta cggcattcgg caaaatccgc gcgatggtcg atgaaaacgg
     8761 caaatccatt accgaagccg gtccgtccat ccccgtcgaa atcctcggct tgtccgacgt
     8821 accgaatgcg ggtgaagacg cgatggtatt ggcggacgag aaaaaagcgc gcgaaatcgc
     8881 cctcttccgc caaggcaaat accgcgacgt gcgccttgcc aaacagcagg cggcgaagct
     8941 ggaaaatatg ttcaacaata tgggcgaaac ccaggcccaa tctttgtcgg tcatcatcaa
     9001 ggcagacgtg cagggctctt acgaggcttt ggcgggcagc ctgaaaaaac tgtccacaga
     9061 cgaagtgaaa gtgaacgtgt tgcacagcgg cgtgggcggc attaccgaat cggatgtcaa
     9121 cttggccatc gcttcgggcg cattcattat cggctttaac gtgcgtgcag atgcctcttc
     9181 gcgcaaactt gccgaaaatg aaaacgtgga aatccgctac tacaacatca tctacgatgc
     9241 catcgacgac gtgaaggcgg cgatgagcgg catgctttct ccggaagaga aagaacagat
     9301 taccggtacg gtcgaaatcc gtcaggtcat ctccgtttcc aaggtcggca acattgcagg
     9361 ctgtatggtt accgacggtg tggtcaaacg cgattcccat gcccgcctca tccgcaacaa
     9421 cgtggttatc cataccggcg agctgtcttc tttgaaacgc tacaaagacg atgttaaaga
     9481 agtccgtatg ggcttcgagt gcggcctgat gattaaaggc ttcaatgaaa ttatggaagg
     9541 cgaccaactg gaatgcttcg acatcgtcga agttgcacgc accctgtgat tttgatgcgg
     9601 aaaaatgccg tctgaagcgt tcagacggca tacgaaacgg gttctgtatc atacagaacc
     9661 cgttttttgt cgcaaatcgg cttcagacag ccctcttgcc ttatcccgat ttgaatctga
     9721 cttgccatac aaacaggctt cagacggcat tatagtgaat taaatttaaa ccggtacagc
     9781 gttggctcgc cttgccgtac tatttgtact gtctgcggct cgccgccttg tcctgattta
     9841 aatttaattc actatatttt cccgacaggc ggatttgaag tttctccaca tatccggcgc
     9901 gttcggcgcg gctggtttcg gggcggtgcg tattgagcga cgaccatttc caatgactgc
     9961 gggctttgtt gagttcgggc gggagtctgg tggcgtccca cggggctttg cggctgtgca
    10021 gctcgatatc cgactgtgcc gcgtgtccgc gcgtttgcag gacgtggagc aaatcgaggg
    10081 cgcgggcggc gagcagggtc agggtttcag ggtcggtgtg caaggtttgg cggccggaga
    10141 gtttgtcgga aatggtgcgg gtattgggca ggatgccgcc caaaaagccg ccgattgccg
    10201 tacccaatcc gagcgagccg ccgagtgtgg cgatgtccag ccccaagccg atgagtgcgc
    10261 cggttgccgc gcccgtgccg gtgcggatgc cgtattgttt gagcaattcg ctgtcgaacg
    10321 ggtcttggcg gaaggcttgc ggcatccagt cgccgccgtc gatttcgctg tggtagaaac
    10381 ggtagagggc aaacagccgc tgctgcatct gccgttcgag ttggcgtatt tccgcctgca
    10441 tggtttgcag cacggtggcg gtatcctcgt tttcgtccac ttcctgcctg aaggcggcgg
    10501 catcgagcag gaaatcggcg atttcgcggc gcgcttcgcc gtccagccgc tgccattcgc
    10561 gccggcgcat ggctgtcagg cggtcaagtg tgctgcgttc gggcaacatg gtggcgaggt
    10621 tttcccacag gcgtagttcg ccttcaaaat caaaggcgac ggtgtcgaac cctgcgaaaa
    10681 cgtgcaggtt tctcctcgcc agcatggttg tccacgattc gggaggctgt ccgccggtaa
    10741 agttgaacac gggcataacc ggtttggcgc accatgaaag gataatcaat tcatcccgat
    10801 atttgtcgag aacgggttcg cgcgcgtcga tgacgtacat tgccatatcg ctttgcaaga
    10861 cttgccgtaa gactttggct tcctgattga aatcatggtg cgcgccgtgg ctgccgagaa
    10921 actgttgcag ccgttcgatg ccgtctgaac gattgtccgt atggttttcc agccattcca
    10981 gcacgccgcc cgcgtcttcg agtccgggcg tgtcgtacag gaaaaccagc gtgtctgcgc
    11041 cgtcgctgat ggcggcttct tcgacatgac gcgtggtcga tggggcgttt ctgacttcgc
    11101 cgaaaccgct gtcgcgcaaa agggtacgca ggagcgaggt tttgccggtg ttggtatgcc
    11161 cgacgacggc gagggaaagg ggttgtttgt tcatgatgtt tttgaagaat ggattttcag
    11221 acgacctttt aaggatggcg gctttatggg gcgtttcaag tgcggtcgtt ggttttcaga
    11281 cggccttcct gcgccgctct gtcgggttcc agccacgccg cgccgcattc ggccagcgcg
    11341 ttacgccaat gttccagctt ttccgaaagg tcgtctgaaa gcccctgttc cgccaaaagc
    11401 tgcaccaccg cgccgccctg cgccgcttcc gagagtcgga caatctgccg caacacaccc
    11461 cggtccggca cagtttgggc gcgcacgccg ataagcagtt gcgccggttt ctgcttcaac
    11521 tccgcttcca gcgcggcgac ctgttcccga ttggcggcaa cacctttatc cagccattcc
    11581 tgcgccagcc tgccctcgaa ccacgcgccg tcctgccact cggtctccaa catgaccgcc
    11641 catttcggtg catcgttcaa gatgattttc ggtgaaacgg cggacacggt ttcccgacgc
    11701 gtatccgcat cggtgatttt gttctgccag cggcggatga ccgcctgata atagggcttt
    11761 tccaaatcca agccgttctc gcttgttttc aaaaggattt tacacactac ccaagccagc
    11821 aggcgcggca ggatgccgta gcaggcgata ctgccgacca gcagccccga ccaagcccgc
    11881 gcatcggcaa tattgccgtt cagacgacct tcgatgaccg ctcgcgcatc aggcacggga
    11941 aaacccagtt tcgcaggcag ccatgccaac atttccacca gccgtaccga agacgaatcg
    12001 cccaacagcg tgctttccca gttgaacgta tattgccgca ccaaaagcag caacaatacc
    12061 gacaccagca ttccgagcag cgtgcagagc cacaggctgt gcgacgttgc gcctattttc
    12121 caacgtaccg aaggttgccg ccactcgtcc gcatacagcc gcaacaccgc ctgatttaca
    12181 gggtctttgc cccgaaacca cgtcgccgga ctgctgaaaa aacgccccac tttcacgcgc
    12241 aggaacaaca ttgccaacca tactgccaac ataagcgtat tcatgcccaa cacgcccgcc
    12301 aaaatcagga agaaattcag cccctgattg tccattagaa gataagtgcc tgaaaatccg
    12361 gcggtaaaca tcatcgatgc cgccaccacc cacaaccaga acgaccccgc acgcacacgt
    12421 tccaacgtct cccgcagcat acggttcctg tcaatcatct ccgcccgacg gatgattttt
    12481 tcctccgtac tgccgtccac gcggcgcaaa gcctccgtcg cctgcacggg atcgccgctg
    12541 aaaataaagc cgccttcttc caaaatacgg accagctcaa ccagttttcg ggatggattc
    12601 aacataaaat gccgtctgaa aataaaaaac agattttaac acacgaattt tcaagaatat
    12661 tcacagtgta ggcaaagagt aaatcccaca cagaggcaaa agtatcggcg taaactgact
    12721 gcctctactt tcccgaaaga ttgtgcgatg tatacaggcg aacgcttcaa tacttacagc
    12781 catttgagcg gtttgattct ggcggcggca ggtttggtgc tgatgctgct gaaaaccata
    12841 ggacacgggg acggctaccg tatcttcagc gtatcggttt acggcatcag ccttcttctg
    12901 ctctatttga gttcctcgct gtaccacgga attgcagccg gaaaactgaa aagcattttg
    12961 aaaaaaaccg accactgcat gatttatgtc ctgattgccg gaagctacac accgtttgca
    13021 ctggtttctt tgagaaacgg gccgggctgg acggtatttt cactgtcctg gctgctggcg
    13081 gctgcaggaa tcgcacaaga actcaccatc ggacggaaaa gcgaaaaacg tctgctgtct
    13141 attgtgattt atgtcgtcat gggttggatg gtcttggcgg taatgaaatc cctgacagcc
    13201 tcactcccgt cggcaggact ggcttggctg gcggcaggcg gtatgctgta cagcgtcggc
    13261 atttactggt ttgtaaacga tgaaaaaatc cgacacgggc acggaatctg gcatctgttc
    13321 gtattgggcg gcagcatcac ccaatttgtc agtgtgtacg gttatgtaat ctgaatgccg
    13381 tctgaaaaac aaaacctccc gatcctgaag attgggaggt tttctgtttg ccggacatca
    13441 gcccttgtcg tggaactcgt ggaattcata cggataagac aaatcccgac ccgctttttt
    13501 ctgtgccaaa taatcatcat aaatggcacg gatttcctta cgcaacaaaa acagggctat
    13561 caagttcggg ataaccataa agccgttgaa catatcggac aggctccaaa ccaaatcgac
    13621 tttgccgagc gtacctaaaa cgatggcaag cagaaccaat gcgcgataga tgcccaagtg
    13681 tcttcccctg aaaagaaaac ggatgttgga ctcaccgaaa taataccagc cgataatggt
    13741 cgtcaaggcg aagaaggtca gacacacggc aagcaattgc gaaccgaagc ccggaaatgc
    13801 cttgttaaag gcaaattgag taaccgccgc gccctgttcg cccgaaaggt tggcatcggt
    13861 cagcaggata atcagcgcag ttgccgtaca taccaaaatc gtatcgataa acacaccgac
    13921 aaatgccgcc ataccttgct gcacaggatg cttcacatcc gcagtcgcgt gggcgtgcgg
    13981 agtcgaaccc atccccgcct cattggaaaa cagaccgcgc gccacgccga aacgtatcgc
    14041 ttcgcgcata ccgatacccg ccgcaccgcc caaaacggct tcgggattga aggcggcggt
    14101 aaagatgtgg ttgaacatcg gcacaatatg gtcggaaaat tcaaacagga taacgacggc
    14161 gcacaaaata taaacaaccg ccataaacgg cacgacaaat tgggcaatat tggcaatacg
    14221 gttcacgccg ccaatcacaa ccatgcccgc aaggacggca agcacaatac cgactgccaa
    14281 agaaggcaca tcaaatgcaa tggtaacggc agaagcaatg gagtttgcct gtgtcgcatt
    14341 accgataaag cccaatgcga taatcaacgc aatggaaaag aaaccggaca aaaaacgcgc
    14401 cgctcccctg ccgattttcg gagtcagacc gtgggtgatg tagaacgccg gcccgccgat
    14461 gtatttgccg tggctgacga cgcggtattt ctgcgccagc agtgcctccg caaaaatcgt
    14521 ggacatcccc aaaacggcag aaacccacat ccaaaaaatc gcgcccggcc cgcctgcggt
    14581 gatggcggtc gccacgccgg cgacgttgcc cgtaccgatt tgcgcggata tggcaaccgc
    14641 caacgcctga aactgcgata aagacttgtc gtctttatcg cctttggcaa acaagccgcc
    14701 gaatacggat ttgaatcccg cgcccagctt ggtaatctgc ggcgcaccaa gatacagcgt
    14761 aaaaaacagg ccgataccca aaagcgcgta aatcagcagg tagtcccaaa ggaaccgatt
    14821 gactgtaccc accagaacag acaatatatt ttccataaaa taaaccttat cttacaatta
    14881 aaatgactgc cttccaaaag acattccaat aaggaaacac ggcgagcaga ccgtatttgc
    14941 cgcaacagat gccttaaatt gtcaacaatc ggggagaagc tgcgccatca taccgtaaaa
    15001 tatcgttaat taaaacattt ctttattttt aagcggaaag cggaggaaat cgctttcaga
    15061 cagcatagac aacggcacgg cataaaacag gatattttgg gtacttgcaa cttatgttaa
    15121 aatgccgacc gtaaaaaatc tgacaaaaac agattaatta tttgaaataa gaaaggaaat
    15181 ttatggcagg ccatagcaag tgggcgaata tccagcataa aaaagcccgt caggatgcca
    15241 aacgcggcaa aatcttcacc cgtttaatca aagaaatcac cgttgcggcg cgtatgggcg
    15301 gcggcgaccc cggtgccaac ccgcgcctgc gcctggcttt ggaaaaagca gccgaaaaca
    15361 atatgcccaa agacaatgtg caacgcgcca tcgataaagg tacgggcaac ttggaaggcg
    15421 tggaatacat cgagttgcgc tacgaaggct acggcatcgg cggcgcggct ttgatggtgg
    15481 actgcctgac cgacaacaaa acccgcaccg ttgcggacgt acgccacgcg tttaccaaaa
    15541 acggcggcaa cttgggtacc gacggctgcg tggcgttcaa cttcgtgcat cagggctatt
    15601 tggtattcga acccggcgtt gacgaagacg cgctgatgga agcggctttg gaagccggtg
    15661 cggaagacgt ggttaccaac gacgacggtt ccatcgaagt cattaccgcg ccaaacgatt
    15721 gggcgggcgt aaaatccgct ttggaggcgg caggttacaa atccgttgac ggcgacgtta
    15781 cgatgcgcgc ccaaaacgaa accgaactct ccggcgacga tgccgtcaaa atgcaaaaac
    15841 tgattgacgc gctggaagac ttggacgacg tgcaagacgt ttacacttcc gccgtattga
    15901 atctggactg atacgcagca cagcagacat acaataaaat gccgtctgaa cctttcagac
    15961 ggcattgatt tatttagccc ttgcccacgc ccaccaaacc gtcaggacaa ccgccagaaa
    16021 atacgccgcg ctccaaacag gcaaagcaac ccctaacaga taatccggtt cggcacaatt
    16081 tccgaacccg cgcacgacag gctcgaacca atcaaacaaa gaccagccct tcaagcggaa
    16141 cgtccacggc gcaccgcacg aaggagccgt acccggcggc agcgactgca accacaactg
    16201 atatgccgca acagaaatac ccgtaacggc cggaatgctg ataaagacag caccgaacaa
    16261 accgcccgcc cttcttcttg gtctgcacat caggacaatt gccgtacaca atgcggttgc
    16321 caaaacgcat aaccgctgac tgatacacaa aacgcaaggc tccataccca aaacatactg
    16381 tgccgccaaa gaaccggcaa atgcacagac cgaaacggca aacagcagcc aaacggcttt
    16441 tctaaataac ggggtcattt tctcaacaca ccaatcaaaa taccgatatg ccgattttgc
    16501 tggatatatc ccgagatggc aagggacgaa tcgacaccgg cgcaggtgcg gacgatttcg
    16561 gttttcccga tatacacaag acctgacaag ggacaaaacg gcgatttgcc cgcacaatgc
    16621 ccgcaaaaaa atacatccgg aggatttgaa tttcggcaaa tttagcgaat caaaagttta
    16681 tttcaatgaa attatatgat ttttttgaat aagcggattg acaggttttt gaaggaattt
    16741 gttaccggat agccatcggg caagtttttt gcaaaatttg aatcggtcgg gtaaattttc
    16801 aaaaaataat tgacagcgga taagaaacgg cggataattc tcgccgtcga gttgcttgat
    16861 gcagcttgat ttttctcctc tatttctcct ttgtagactt ggcacacatt caaccggatg
    16921 tgtgcatttt tttatctccg ctttttttga aatttaatta cttttattgt ttgaaattcc
    16981 attctttaag aaatttaaca agaaaaacgc ttgctttttg accggaaaag ctgcataatt
    17041 cgttccgtta gctggttcga gtagtcagtt aatagtttct cctctatttc tcctttgtag
    17101 acttggcaca cattctattg aatgtgtgca tttttttatc tgaagcaaca agcctctgtg
    17161 cgtgatgttg ttatgtttca tttaggtgtc aaaccgcata tccggtctga aatattcaat
    17221 ccaaatccaa aaccggattt tctttgacct cctccatcac aacataactc ctactctccg
    17281 aagcggcagg cagttgcaat aggatattgc ctagcatatc ccgataggca gacatatcgg
    17341 gcaaacgtac tttaatcaga tagtcgtatt cgcccgacac caagtggcat tccataattt
    17401 gcggaatttt cagcacttct tttttgaaat cttcgaaaat attgcccgat ttggattgca
    17461 gcttcagctc gacaaaaacc aatagtggtt tgcccaacag atggggattg aggtgggcgt
    17521 gatagccgga aatataatgt tcccgctcca aacggcgcac cctctctgta acgggcgtgg
    17581 tggacaagcc taccttctcg gcaagctccg tcatcgggat gcgggcattc tgttgaagga
    17641 tcttaagaat gcggaaatcg attttatcta gttctttcat ttggattgcc ttgtatttat
    17701 tattgatttt aacaaataga g
//
LOCUS       DAXHMX010000024        17107 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHMX010000024 DAXHMX010000000
VERSION     DAXHMX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.024"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..484)
                     /locus_tag="ACFM4S_000617"
                     /pseudo
     CDS             complement(1..484)
                     /locus_tag="ACFM4S_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            586..896
                     /locus_tag="ACFM4S_000618"
                     /pseudo
     CDS             586..896
                     /locus_tag="ACFM4S_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(953..2011)
                     /gene="trpD"
                     /locus_tag="ACFM4S_000619"
     CDS             complement(953..2011)
                     /gene="trpD"
                     /locus_tag="ACFM4S_000619"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6050349.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFAAKVPLEDAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRYVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(2075..2665)
                     /locus_tag="ACFM4S_000620"
     CDS             complement(2075..2665)
                     /locus_tag="ACFM4S_000620"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6050350.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPECLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3086..5362
                     /gene="znuD"
                     /locus_tag="ACFM4S_000621"
                     /gene_synonym="tdfJ"
     CDS             3086..5362
                     /gene="znuD"
                     /locus_tag="ACFM4S_000621"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC6050351.1"
                     /translation="MAQITLKPIVLSILLISTPLLVLAHETEQSVDLETVSVVGKSRP
                     RATSGLLHASTSSDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVTD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGNNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVVYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGSHDGDGAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPLPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNHF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYIKTDNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(6023..6820)
                     /locus_tag="ACFM4S_000622"
     CDS             complement(6023..6820)
                     /locus_tag="ACFM4S_000622"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6050352.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTEVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLAAP
                     QIESEPESEPALQTAPVETAATPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            6975..9821
                     /gene="uvrA"
                     /locus_tag="ACFM4S_000623"
     CDS             6975..9821
                     /gene="uvrA"
                     /locus_tag="ACFM4S_000623"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6050353.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVITGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAIEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            complement(9853..10023)
                     /locus_tag="ACFM4S_000624"
     CDS             complement(9853..10023)
                     /locus_tag="ACFM4S_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050354.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(10038..10223)
                     /locus_tag="ACFM4S_000625"
     CDS             complement(10038..10223)
                     /locus_tag="ACFM4S_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050355.1"
                     /translation="MTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKAPPTQ
                     NPGSSWIQIKRTPDSSR"
     gene            complement(10239..10508)
                     /locus_tag="ACFM4S_000626"
     CDS             complement(10239..10508)
                     /locus_tag="ACFM4S_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050356.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            10664..11587
                     /locus_tag="ACFM4S_000627"
     CDS             10664..11587
                     /locus_tag="ACFM4S_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFC6050357.1"
                     /translation="MKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKINRKDLMQ
                     AFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIKILNGSL
                     IFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGLSEKVIV
                     PLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDIDWNRIV
                     KIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDHHIDDPA
                     FRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            complement(11814..12080)
                     /locus_tag="ACFM4S_000628"
     CDS             complement(11814..12080)
                     /locus_tag="ACFM4S_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050358.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            12329..12658
                     /locus_tag="ACFM4S_000629"
     CDS             12329..12658
                     /locus_tag="ACFM4S_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6050359.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRQHERGIWQRRFYGHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKK"
     gene            complement(12724..12864)
                     /locus_tag="ACFM4S_000630"
     CDS             complement(12724..12864)
                     /locus_tag="ACFM4S_000630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050360.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFEAGRIFIGQTGA
                     NP"
     gene            complement(12893..13783)
                     /gene="sucD"
                     /locus_tag="ACFM4S_000631"
     CDS             complement(12893..13783)
                     /gene="sucD"
                     /locus_tag="ACFM4S_000631"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6050361.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(13794..15005)
                     /gene="sucC"
                     /locus_tag="ACFM4S_000632"
     CDS             complement(13794..15005)
                     /gene="sucC"
                     /locus_tag="ACFM4S_000632"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6050362.1"
                     /translation="MEANSGHSTNLKEKSMNLHEYQAKELLASYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            complement(15059..15346)
                     /locus_tag="ACFM4S_000633"
     CDS             complement(15059..15346)
                     /locus_tag="ACFM4S_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050363.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(15471..16904)
                     /gene="lpdA"
                     /locus_tag="ACFM4S_000634"
     CDS             complement(15471..16904)
                     /gene="lpdA"
                     /locus_tag="ACFM4S_000634"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6050364.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDIKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLNIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         4335 a         4580 c         3894 g         4298 t
ORIGIN      
        1 atggtattgt tttgtccgcc aaaatggacg cgaccgtata ggacgtcttc cgaacccatc
       61 aaccaacagc gctcataaaa acgaccgccc gagcctttgg actctttgta gagaccgaaa
      121 ccgaaaactt cttcggcgag catggacgcg gcgtgaatga aatcttcatc ttctaacaga
      181 ctgactctaa caccgtattt atcgaaaaag gttttttcat gaaatgaaaa gctaatttga
      241 tcaatgaaag ccgaatctga tacaccgcgc cgaagaggaa cgcctaacag gtttccttta
      301 ccgtccgtta tgtacgtttc gtaacattcg aagacttcct gaaccctgcc tgccgtttcg
      361 gtttctgtac ccccccgtta gataaggggg gaaggtttga agcggttgcc ggcttcctgc
      421 cgtccgtcgg cgcgtccgtc atcacgccgg caaccgcctt tgtcatccct tgcttatctt
      481 ccatggtgcg aatcctcaaa aacgggcaaa aaaaagccct gttacttgta gaaagtaaag
      541 gacgttaatt tttgttaatc gtcccttctt agggacgcaa tatataaggt ttataccgtc
      601 gttgttccta atgcgcaatc agcgacattg ttgccaatta tccgaaagaa agttaagcct
      661 gatggcattg tatacacgga tacctttcgt agttatgatg tgcttgatat tagtgaattt
      721 agccatttac gtaagtttaa cggcattccc aaagagcatt tggggctgca tttaaagaaa
      781 tgccaatggc attttaaata gaatctcaaa ttcagatttt aagacaatta gttaatggga
      841 atttggtctg gttatctagt acagccccaa gtttttatat aaaaacagtt tggtaagttc
      901 tcttcgtgaa gtgcttatgt tttgccacaa aagttccata atcatggcgg catcacccaa
      961 gctccatctt tccaagaaaa tggcatcttt gttgtggttg aaaaccgaca aactcctctt
     1021 ttttcgattt ggccctgcct gaatcgatgg cttcccgtgc ggcagatatg ccgtctgaaa
     1081 gcgaagcagc gacatttccg gcatacaggg cggcggcggt gttgagcaat acgatatcgc
     1141 gcgcagcccc ttctcttcct tccagcacct cattcatttt caacaaagat tcctgagtat
     1201 tggcaacttt gatttcatcc aaattgcggc gggtttcgat accgaaatct tctgggcgga
     1261 tgtcgtattc gctgattttt ccgtctttga gctcggcaac gcgtgttttg cccgtcagtg
     1321 taatttcatc caaaccgccc tccccgcaaa caaccaaaac atgcttggag ccgagctgtt
     1381 gcaatactct ggaaaggatg ccgcacaaat cggtgtggaa cacgcccaaa agctggttcg
     1441 gcgcgcccgc aggattcgtt aacggaccca atatgttgaa aatacttcgg aaaccgagcg
     1501 aacgtcgtac cggggcaaca tagcgcatgg cactgtggtg attgggggcg aacataaacc
     1561 cgatgccggt ctgcctgata ctttgggcaa tctgttcggg agtcagattg aggtttgcgc
     1621 ccatttgctc catcacgtca gccgcaccgc tggaggaaga gaccgaccgg cctccgtgtt
     1681 tggcaacctt cgcgcctgcc gctgcggcaa caaacatcga agtcgtcgaa atattgaagg
     1741 ttttcgcgcc atccccgccc gtaccgacga tatcgaccag cccttccgca tcctccagcg
     1801 gcacttttgc cgcaaactcg cgcatcacgg cggcggcagc ggtaatctcg gaaacggttt
     1861 caaccttgat acgcaagccg gtcaaaatcg ccgcaatttg ttcgggcggc acttttccgc
     1921 tcataatctg acgcatcaag tcggtcattt catcgtaaaa caactcgtta ttgctgatta
     1981 atcgttcgat ggcctgttgc ggtgtaatca ttttttgtcc tccgttcaat attcggacga
     2041 aaatgccgtc tgaagggctt cagacggcat cacgtcagat tttttgcggt ttgaagtttt
     2101 gaaattcgat taaaaaattg tttaacatat catgtccgtg ttcggtcaag agggcttcag
     2161 ggtggaattg taccccctca acggcatatt ccttatggcg cacgcccata atctcgccgt
     2221 cttccgtcca tgccgtgatt tccaaacatt cgggcagcgt gccgcgttcg ataacgaggc
     2281 tgtgataacg cgtacaggta accggattgg gcaaaccctt aaacatcccc ttgcccgaat
     2341 gggacacggg cgacacctta ccgtgcatca gcgttttggc gcggactatc ctgccgccga
     2401 acgcctcgcc tatcgtctga tgcccgaggc acacacccat aatcggcagc ctgccggcga
     2461 aatggcgcat cgccgccacg gaaatccccg cttctttggg cgaacacgga ccgggtccga
     2521 taacgagata ttgcggattc aatgcctcga tttcctccaa cgtaatatca tcgttgcggc
     2581 gcacggcaac ttcctgcccc aattctgcga aatactggac gatgttgtaa gtaaaactgt
     2641 cgtaattgtc gataaacaaa agcattttgt gattaaccta ttgatttata atttatttat
     2701 tattaactgc attttcgata cccccattca taccccttat atcttagcgt gcccgatgcg
     2761 ccctcgtgaa tctggcgcag agcctcgcgt tctgacgagt acgccatttt gccacctttt
     2821 cgagcgttcg ggaacgtgcc gtttttatgc actcataaat tttggttatc ccgcagccga
     2881 acataatgga caaacacaac ggaatccgca tcaacatcaa ccgcgccccc gaatgacgga
     2941 ggatacaaat ggacaccgcc tgatgccgca accgtgtcat ttgaaaaact tttgcctatg
     3001 ccctctgagt gttctcaaaa tcttatgata taaaaaatgc aatgttatat aataacaaac
     3061 ttttaaaatg cgaagagaag ccaaaatggc acaaattaca ctcaaaccca ttgttttatc
     3121 aattctttta atcagcacac ccctcctcgt cctagcgcat gaaaccgagc agtcggtgga
     3181 cttggaaacg gtcagcgtcg tcggcaaaag ccgtccgcgt gccacttcgg ggctgctgca
     3241 cgcttcgacc tcctccgaca aaatcatctc cggcgatacc ttgcgccaaa aagccgtcaa
     3301 cttgggcgat gctttggacg gcgtaccggg cattcatgcc tcgcaatacg gcggcggcgc
     3361 gtctgctcct gtcattcgcg gtcaaacggg caggcggatt aaagtgttga accatcacgg
     3421 cgaaacaggc gatatggcgg atttttcgcc cgatcacgcc attatggtag ataccgcctt
     3481 gtcgcaacag gtcgaaatcc tgcgcgggcc ggttacgctc ttgtacagct cgggcaatgt
     3541 ggccggtttg gtcgatgtta ccgatggaaa aatccccgaa aaaatgcctg aaaacggcgt
     3601 atcgggcgaa gccggattac gtttgagcag cggcaatttg gaaaaactga catccgcagg
     3661 catcaatatc ggtttgggca acaacttcgt gctacatacc gaaggcttgt accgcaaatc
     3721 aggcgactac gccgtaccgc gttaccgcaa tctgaaacgg ctgcccgaca gccacgccga
     3781 ttcgcaaaca ggcagcatcg ggctgtcttg ggttggcgaa aaaggcttta tcggcgtggt
     3841 gtacagcgac cgcagagacc aatacggcct gcctgcccac agccacgaat acgatgattg
     3901 ccacgccgac atcatttggc aaaagagttt gattaacaaa cgctatttgc agctttatcc
     3961 gcacttgttg accgaagaag acatcgatta cgacaatccg ggtttgagct gcggttctca
     4021 cgacggtgac ggtgcacacg cacacaccca cagcggcaga ccgtggatag acctgcgcaa
     4081 caaacgctac gaactccgtg ccgaatggaa gcagccgctc cccggttttg aagccctgcg
     4141 cgtacacctg aaccgcaacg actaccacca cgacgaaaaa gcaggcgatg cagtagaaaa
     4201 cttcttcaac aacaaaacac acaacgcccg tatcgagttg cgccaccaac ccataggccg
     4261 tctgaaaggc agctggggcg tgcaatattt gggacaaaaa tccagcgcac tttccgccat
     4321 tcccgaaacc gtccaacaac cgatgttgat tgacaacaat gtgcgccatt acagcttttt
     4381 cggtgtagaa caggcaaact gggacaactt cacgcttgaa ggcggcgtac gcgtggaaaa
     4441 acaaaaagcc tccatccgat acgacaaagc attgattgat cgggaaaact actacaacca
     4501 gcccctaccc gatctcggcg cgcaccgcca aaccgcccgc tcattcgcac tttcgggcaa
     4561 ctggtatttc acgccgcacc acaaactcag cctgaccgcc tcccatcagg aacgcctacc
     4621 gtcaacgcaa gagctgtacg cacacggcaa acacgtcgcc accaacactt ttgaagtcgg
     4681 caacaaacac ctcaacaaag agcgttccaa caacatcgaa ctcgcgctgg gctacgaagg
     4741 cgaccgctgg caatacaatc tggcactcta ccgcaaccat ttcggcaact acatttacgc
     4801 ccaaacctta aacgacggac gcggccccaa atccatcgaa gacgacagcg aaatgaagct
     4861 cgtacgctac aaccaatccg gtgcggactt ctacggcgca gaaggcgaaa tctacttcaa
     4921 accgacaccg cgctaccgca tcggcgtttc cggcgactat gtacgaggcc gtctgaaaaa
     4981 cctgccttcc ctacccggca gggaagatgc ctacggcaac cgtcctttca tcgcacagga
     5041 cgaccaaaat gccccccgtg ttccggctgc gcgcctcggc ttccacctga aagcctcgct
     5101 gaccgaccgt atcgatgcca atttggacta ctaccgcgtg ttcgcccaaa acaaactcgc
     5161 ccgctacgaa acgcgcacgc ccggacacca tatgctcaac ctcggcgcaa actaccgccg
     5221 caatacgcgc tatggcgagt ggaattggta catcaaaacc gacaacctgc tcaaccaatc
     5281 cgtttacgcc cacagcagct tcctctctga tacgccgcaa atgggtcgca gctttaccgg
     5341 cggcgtgaac gtgaagtttt aaaactagat aggcaaacaa aaaaggtttt cagacggcat
     5401 tctgaaaacc ttttttcttt ttaatcaatt tgttaggaaa tatgacaaag acaaagaaca
     5461 aaccactatg ccatcattcc catttttttg tttcatcatt cttgctttca cgccgtcatt
     5521 ctcgcaaaag cagaaaccca tcattcccgc gcaggcggga atccaattca ttcggtttca
     5581 gccgtttccg ataaatcgcc ttagcatttg ggttctagat tcccactttc gtgggaatga
     5641 cggcgtaaag gtttctgttt tttaagtgtt aggtaactaa taagtcgtca ttcccgcgca
     5701 ggcgggaatc caattcgttc ggtttcagcc gtttccgata aatcgcctta gcatttgggt
     5761 tctagattcc cactttcgtg ggaatgaggc ggaaaggttt ctgtttttta agttttaggt
     5821 aactaataag tcgtcattcc cgcgcaggcg ggaatacaat tcgttcggtt tctgttattt
     5881 ctgataaatc accgtaacgt taagttccca gatttccact ttcgtgaaca taacggtgtg
     5941 aacattagca aaaattcaaa aaaccaaact tcctcattcg tgtgaatgat ggtactgata
     6001 ttttctattt cagttcgcaa aattaatctt tcaacacatc ttgcacagcc gcctgaatct
     6061 tagccgccgc tgcctcgatt tgccgttgtt cggcagatgg ggttgccgct gtttcaaccg
     6121 gagcagtttg taaagcaggc tcagattcag gctcggattc gatttgcggc gcagccagcg
     6181 gcaaacgcgc caatacagtg ctgacgccgg ttactttatc gccaatcgcc acttgcgcct
     6241 gcgcatcgac aggcagatac atatccacgc gcgaaccgaa gcgaataaag ccatagcgtt
     6301 cgccgcggga cagtttcgca cctgcttggg tgtagcacaa aatacggcgc gccaccaaac
     6361 cggccacttg aacaaaagta atttcacgac cagaagccgt cgtcgccaaa accgcgttgc
     6421 gttcgttttc cgtgctggct ttgtccaaat ccgcatttac gaatttgcct ttgttataga
     6481 ccacttccgt taccgtacaa tcggcaggcg atttttgcga atgcacgttg aacacgttca
     6541 taaaaatact gattttcaaa gcatcgacat cacgatacgg atcgcgtgcg cgctcgacca
     6601 ccacaatgcg gccgtcaaca gggctcaaca ccgcttcagg attttgcgga atctcacgcg
     6661 cagggtcgcg gaaaaactgc aatgcaaata cggtaaacac ccaaaacggc aaagaccacc
     6721 agccgcagca catcgacacc agcaggctca aagccaaacc gccgccgata atcggccagc
     6781 cctcacgggc gataatcggg tgggggtaaa gacggttcat ttgtcatcct tctattcaag
     6841 cgggttaaaa ttgccgccat tataacggaa tccgccgcat tccgccgaca aatgccgtct
     6901 gaacgcggct tcagacagca tccgagaaag tgctacaata tcggttttcc tttatttttc
     6961 aggcggcatc cattatgtgc aaccatcatc cccgacattc gcacgacaac gacaccatcc
     7021 gcatacgcgg cgcacgcacg cataatttga aaaacatcga tttggacatt ccgcgccaca
     7081 agctcgtggt cataacagga ttgtcaggca gcggcaaatc atcgctggca tttgacacgc
     7141 tgtatgccga agggcagcgg cgttatgtcg agagcctttc cgcctatgcc cgtcaatttt
     7201 tacagatgat ggacaaaccc gatgtcgatt tgatcgaagg cctctcgccc gcgatttcca
     7261 tcgagcaaaa atccaccagc cacaatccgc gttccaccgt cggcacggta actgaaatcc
     7321 acgattacct gcgccttttg tacgcccgcg tcggcacgcc gtattgcccc gaacacaatc
     7381 tgccgctatc cagccagacc gtgtcgcaga tggttgatgc cgtgttgaag ctgccggaag
     7441 acacgcgcgt gatgattctg gcgccggcgg tacgcgagcg taaaggtgag tttgtcgatt
     7501 tctttgccga cttgcaggcg cagggctttg cacgggtgcg agtggacggc gaggtctatc
     7561 agcttgacga agtgccgaag ctggaaaaaa acatcaagca caatatcgat gtcgtcatcg
     7621 accgcgtgaa agtaaaagct gacatcaagc aacggctggc agaaagtttt gaaaccgctc
     7681 tgcgccacgg caacgaacgc gcactggcga tagagatgga cagcggcgaa gagcattggt
     7741 tttccgcgcg tttcgcctgc cccgtatgtt cgtacagcct gcccgaattg gagccgcgcc
     7801 tcttttcgtt caacaacccg atgggctcct gcccgacttg cgacggcttg ggtaacacca
     7861 atttcttcga ctccgaaaaa gtggtcgcgc atcccgaatt gtcgctggct tcgggcgcga
     7921 ttgacggctg ggacaagcgc aaccagttct atttccaaat gattcagtcg ctggcgcggc
     7981 attacggttt cgacgtgcag gctgcttggg aaaccctacc tgcaaaaatc aaaaaagtcg
     8041 tgttgcacgg ctcgggcaaa gaagtcattg atttcactta cctgtccgaa cgcggcacca
     8101 ccttcaaccg cagccacgcc ttcgaaggca tcatccccaa tctcgaacgc cgctaccgcg
     8161 aaaccgacag cgaaaccgtg cgcgaaaaac tgcgcgaata ccaaaaccac cgcgcttgcc
     8221 cgagctgcgg cggcgcacgt ttgcgcaaag aagcccgcta cgtttacgtc ggcggcgagc
     8281 cgttgcacga agtttccgcc tggccgctga ccaaaaccca ccgattcttt gaaacgctgg
     8341 atttggacgg caacaaaaaa caaatcgccg aaaaaatcct caaagaaatc accgaacgtc
     8401 tcggcttttt gattaatgtc gggctggatt acctgaatct ctcccgttcc gccgaaaccc
     8461 tttccggcgg cgaagcccag cgcatccgcc tcgccagcca aatcggcagc ggcctgaccg
     8521 gcgtgatgta cgttttggac gaaccctcca tcggcctgca ccagcgcgac aacgaccgcc
     8581 tgcttgccac cctcaaacgc ctgcgcgatt tgggcaacag cgtcatcgtg gtcgaacacg
     8641 acgaagacgc catccgcgaa gccgatttcg tcgtcgatat gggcccgggc gcaggcgaac
     8701 acggcggcaa cgtattgatt gccgacacgc ccgaaaacgt cgccaaatgc gaaaactccg
     8761 ttaccggaca atacctcagc ggcaaaaaat ccattgccgt gccgtctgaa cgcacgccag
     8821 tcaatcccga ccgaatgctc gtcctcaaag gcgcgcgcgg caacaacctc aaaaacgtta
     8881 cccttgagct gccgctcggt ttgattacct gcatcaccgg cgtatccggc agcggcaaat
     8941 ccaccctgat taacgacacc ctcgccaaaa tcaccgcccg cgaactcaac cgcgcccaag
     9001 aagaacccgc cccatacgac gacatccgcg gcctcgaaca cctcgacaaa gtcatcaacg
     9061 tcgaccaatc ccccatcggc cgcaccccgc gctccaaccc cgccacctac accggcctgt
     9121 tcacccccat ccgcgaactc ttcgccggtg tacccctctc gcgcgaacgc ggctacaacg
     9181 tcggcagatt ctccttcaac gtcaaaggcg gccgctgcga agcctgccaa ggcgacggcg
     9241 tcatcaaagt cgaaatgcac ttcctgcccg acgtgtacgt cccctgcgaa gtctgccacg
     9301 gcaaacgcta caaccgcgaa acgctcgaaa tccaatacaa aggcaaaaac atcagccaag
     9361 tcctcgacat gaccgtcgaa gaagcccgtg aatttttcga cgccgtcccc accgtatcgc
     9421 gcaaactgca aaccttgatg gacgtaggct taggctacat ccgtctcggc caatccgcca
     9481 ccaccctctc cggcggcgaa gcccagcgcg tcaaactcgc cttggaactc tccaaacgcg
     9541 acaccggcag aacgctctac atcctcgacg aacccaccac cggcctacac ttcgccgaca
     9601 tcgccctgct gctggaagtc atagaccgtc tgaaaggcaa aggcaactcg atagtgatta
     9661 tcgagcataa tctggatgtg attaaaaccg ccgattggat tgtggactta gggccagaag
     9721 gcggcgacgg cggagggagg attattgctt caggtagccc ggaggaagtg gcgaaggtta
     9781 aggggagtta tactgggaag tatttgaaga atatacttta aaaaacggga tggtttaaac
     9841 catcccgttt ttttattcct tctgttttcg attttgtttg gctttgaagc tatcaaaaat
     9901 aatgctaaat cccaaacagg tgaacattaa aggcatcccc catttcatat ccgaagaaat
     9961 aacattaaac atttctaata atgtaagaat gcctgataaa gctataaaaa tacctaaaaa
    10021 cattagaact atccttatta tctggagcta tctggagttc ttttaatttg aatccatgag
    10081 cttcctggat tttgagtggg tggtgctttt tgattatctt taatggttgc gtagttatct
    10141 tttcgaccac ctcgtgaacc aatttcttga taaattccac cgttatttcc tgtattttga
    10201 ccaggtttga ttttgttcgt cattatcatc tccataattt aaatagttaa agtatgagtc
    10261 cattgcggat accaatttgc agataaaaaa tctactagat ttttgtctcc atagaattgt
    10321 gtacgcccat gttgttcaaa tattaacgca gattggtcaa tttttaatcc atttctacga
    10381 gcttcagtgg tcaattcctt cagtacgaca ctatggttac cattagttga tttagcatca
    10441 aaaacagcaa agttaatata tcctccacct acggctggat gtcttaagtg agcaaatttt
    10501 attttcataa ataaatccta taaaaaatta cataacagaa attttaagtc agagagctac
    10561 tatgaagtag ctttttgcat tatatgcact ctttttgatt acgtcaagca atttttttga
    10621 attcatattg ccatgaaaaa gattgatgga atataatgca attatgaaaa caatcgctcc
    10681 ttcccatata aagcccctta tgccagagtt gaaccaatct caacgcaata ggattatatt
    10741 tattgatttc tatctatgct ttctaggaaa gataaataga aaagatttga tgcaggcatt
    10801 tcaaatttcc ccagctgttg ctactaggga ttttgcgcta tattgctcta gtttttcgaa
    10861 aaatctccgt tttgatacca aaagtaaatt ttacctctct actgaagact ttgaacctca
    10921 attcccccat tcatttgaaa atgcaattaa aattcttaat ggcagtttaa tatttgatat
    10981 tgattcccaa catcaacctc ttcttccgtt tgatttacct cctgttttaa taaatcctga
    11041 tgtcttaatc ttttctaaaa tatcgaaagc attatcctta aaaaaacctc taaaaatctc
    11101 atatcattca atttcatctg gattatctga gaaagttatt gtgcctcttg ctctagttaa
    11161 tgacggattt cgatggcatt tacgggcatt cgatagaaca aaaaaccgat ttgcagattt
    11221 tgtcctaaca cgaataattc aagcaaatat tttattagct gaaaatattg aagaaaatga
    11281 aacgattaac aatgatattg attggaatag aattgttaaa atcgagttgg ttccccatcc
    11341 taatcatgat gaagctaaaa aagtgattga aatggacttt ggaatgacaa atggaaaatt
    11401 agaattggcg cttagagctt caaatatcgg atatgtttta cagcaatggc gagtcgattg
    11461 ttcgtcagat catcatatcg atgatcctgc ttttagatta tggctgttag ataatttagt
    11521 tttatacggc atcaacaatg ctgaacactt agcgcctgcc tttgataaaa aacgtagcaa
    11581 gccgtagcct acatatccaa ccgaccgcgt gcgtggctgc gccacacacg cggttgtttg
    11641 attgatggct ttctgttgta aattgaactt agttaaaact aacactttca gacggcatga
    11701 aaaatggccg tctgaaaact gaaaaagctt tatctaaaac aactacccta actcacaaaa
    11761 caaaaaacgg attcccaatc attggaatcc gtttttctgt aaagctatcc gaattattta
    11821 gcttcttctt tagcgtcttc agcagcttct ttggcatctt ccgctgcgtc ttttgcttct
    11881 tctttagcct cttcagcggc gtctttggct ttttcttcag gagtttctgc agcagcaagc
    11941 cgtagcctgc atacccaaca ccgcgtgcgt ggctgcgcca cacaccctac ctgtgaatta
    12001 cacggaactt caaaatcaac gtgtagggtg tgtgcggtac gcacgcacgc ggttgcttga
    12061 ttgacggttt gccgtttcaa cttttaactt cgttgaagct agcgctttca gacggcctcc
    12121 aatgtcgtct gaaagcgcta gcttcaacga agttaaaaac cttaaacaac aagccattcc
    12181 ttctccaacg ctcaaaccaa aaacaaaaat cctatggcgc gttatcgcag aaacttcatt
    12241 gccggcggca cattcttttt caccgtcaaa ctcgccgacc cgaaatcgcg cctgcttgtc
    12301 gaacatatcg acttcgcggg cggcttatat ggatgtgcaa aaacaatatt cctttgaaac
    12361 cgtcgccgta tgcgtgctgc cgaaccatat tcacgccatt tggacgctgc cgcccgacga
    12421 tgcggattat tccctactcc ggcggctgat taaaaccaaa ttctccgcat attcccctta
    12481 tactaaaaac ttatccgccg gcaaacagcg gcagcacgaa cgcggtattt ggcaacggcg
    12541 tttttacgga cacaccgtgc gcgacgaaac ggatttgcag cgttgcgcgg actacatcca
    12601 ttccaatcct atcaaacaaa aaatgtcgcg tgctaaaaca gaatcctgca aaaaataata
    12661 tcagtctttt caaatgcgat agttatcatt ccccaacttc aaacaaggcg tgtgcaacct
    12721 gacttacggg tttgctcctg tttgaccgat aaatattctg cctgcctcaa agggcaaaaa
    12781 taatgtgggt atgttgaggc tgcctctttt tgaaatgaca aacaaaatgc cgtctgaaac
    12841 ccgatttccg gtttcagacg gcatttgatg ttcaatcagt tgtcaaacct gattatgcca
    12901 aaccttttgt tttcaacact tccagcatgg tagtgcccaa ctcggcaggg ctgcgggtgt
    12961 aagcgatacc ggctttttca aacgcggcga atttttcctc ggcagtacct ttgccgccgg
    13021 aaataatcgc accggcgtgg cccatgcgtt tgcctttagg agcggtaaca ccggcgatgt
    13081 agccgacaac aggtttgctt acgttggatt ggatgtattc ggctgcttct tcttccgcag
    13141 taccgccgat ttcaccgatc atgatgatgg cgtcggtatc tgggtcttct tggaaaagtt
    13201 tcagtgcgtc gatttggttc ataccgggaa tcgggtcgcc gccgataccg atacaggttg
    13261 attgacccaa gcccagtttg gtggtttgtg ccacggcttc gtaagtcaat gtaccggaac
    13321 gggaaataat accgatgcgg cccggagtgt ggatgtggcc cggcataata ccgattttgc
    13381 actcgcctgg ggtaatcacg cccgggcagt tagggccgac caagcgggta ccgttgccgt
    13441 tggtttccaa gtaacgtttg gctttgagca tgtccagagt cggcacgcct tcggtaatca
    13501 caaccaccaa gcctacgccg gaatcaacgg cttcaacgat agaatccaac acaaacggag
    13561 cgggaacgta aattacagat gcatccgcgc cggtttcttt aacggcttct ttcatggtgt
    13621 tgaatacagg caggttcagg tgggtttgac cgcctttgcc cggggtaacg ccgccgacaa
    13681 ctttagtgcc gtaagccaga gcttgttcgg agtggaaagt accgttttta ccggtgaaac
    13741 cttgaaccaa tactttggtg tctttattaa tcaatacgct cattcttttc tccttaggcg
    13801 tttacggctg caacaatttt ttcggctgcg tcattcaggc cgtctgcaga agtcagtttc
    13861 agacctgatt cgttcaggat tttcgcgccg agttcggcgt tgttgccttc caaacgaaca
    13921 acgacaggaa cgtcgacgtt gatttcttta acggctgcca cgatggcttc cgcaatcatg
    13981 tcgcaacgta cgataccgcc gaagatgttg atcaatacgc ctttaacgga tttgtcttcc
    14041 aaaatcagtt tgaacgcttc aaccacgcgg tctttggttg cgccgccgcc aacgtccaag
    14101 aagttggcag gttgaccgcc tttgagtttg atgatgtcca tggtggccat cgccaaaccg
    14161 gcaccgttaa ccatacagcc gatgttgcct tccagggcaa catagttcag gtcgaattca
    14221 gaagctttca gttcacgttc gttttcttga gatttgtcgc gcaattcagc gatttttggc
    14281 aggcggtaga gcgcgttgct gtcgatgccg attttgccgt ccacgcaggc gagcgcgccg
    14341 ttttcgcgaa ctgccagcgg gttgacttca aacagggcga agtcgttttc gacaaacgct
    14401 ttgtacgcgc cggtcatcag tttgacgaac tcgttgattt gtttgtcttt caagcccagt
    14461 tggaaggcaa cttcgcgagc ttggcaaggt tgcaggccga ccagcggatc aacggttact
    14521 ttgaagattt tttctggagt ttcggcagca actttttcga tttctacgcc accttcggta
    14581 gaagccatga atgtaacgcg gcgggtagaa cggtcaacca ctgcgcccaa gtacagctcg
    14641 gtttgaaccg gatacatatc ttcacaaacc aaaacgctgt tgacaggttg gccgttggca
    14701 tcggtttggt aagttaccaa gttggtgcca atcaggcttt cagccacttc tttagcttct
    14761 tcgcggcttt taacgacttt tacgccgccc gctttaccgc ggccgccggc atgtacttgt
    14821 gctttgacaa cagcgaattt gccgcccaat ttgtcgtaag ctgcagcggc ttcttcgccg
    14881 ttgtgtgcca aaataccgcc ttgtacgggc aaaccgtagc tagccagcag ttctttagcc
    14941 tgatactcgt gtaaattcat ggatttctcc tttagattag tggaatgccc cgagtttgcc
    15001 tccaaatggc aagcccgtgc gtttcagact acctttcggc ggtcggaaaa tcgtcgtttc
    15061 acttgctgaa aggcaaccac atccgcgcca gcagatacat ggctgcaccg cctcccccga
    15121 gaataaaaaa gacgatgcct tttttacgcg aattcggaca gagcagataa acggcaaggc
    15181 tgaaactgat aaacaacaat gccacgcgcc aaaccaattc tttatctttg aaacggttga
    15241 gcgtataagt ttcggtcatc atcacataca tctgccacaa tgcggccata cacatgctga
    15301 tgatgcccat cggtataaag aaaataccga ctatttcttt gttcataacg aatgtaaatg
    15361 ctgtttgttt gtctgacgga attataaatc agaaaacgaa gctctcggaa cattcaattt
    15421 gtcgaaagcc ttaaggccgt ctgaaaagtt tcagacggcc ttaatatcta tcaaccgtgc
    15481 aaagcgcgtt tgtcggccgc caatgcagct tcgtgaacca cttcggacaa ggtcgggtgg
    15541 gcatggataa tgcgggcgat gtcttcgctg ctggcgaaga attcgagcgc agtcacgcct
    15601 tcggtaacca attcgctgac aaccggacca atcatgtgta cgcccaagat gcggtcggtt
    15661 ttggcatctg ccaatacttt aaccgtacct ttggctttgc ccattgccaa tgcgcgaccg
    15721 tttgcaccga aacctgaagt accttttttg tactccacgc cttcggcttt gagctgctct
    15781 tcggttttac ctacccaagc gatttcagga tcggtgtaaa tcacgaacgg tacgttgttg
    15841 aagtcgatat gcggtttttg accggcgatg cgctcggcaa cggcaacgcc ttcatcgctg
    15901 gctttgtgtg ccaacatcgg gccgcgaacc acgtcgccga ttgcccatac gttaggcagg
    15961 ttggtacggc attcgccatc tactttgatg aaaccgcgct cgtctttttc caagcctacg
    16021 gcttcggcgt tcagaccttt ggtgtttgga atacggccaa tggcaacgat cagtttgtca
    16081 aatacttcgg ttttagcttc gccggcagca gtttcgtaag caacggaaac gcctttgcct
    16141 tcagatttga tgtcgccaat tttcacgccc agttcgatgt tcaagccctg ctctttagtg
    16201 aagtatttga aggcttcttt ggcgatttgt tggtcggcgg cagccaggaa ggtcggcgca
    16261 gcttcaagaa tggtaacttc tgcacccacg cggttccata cggaacccat ttccaaacca
    16321 atcacgccgg aaccgatgac gcccagtttg gcaggcactt cggtcaggtt caatgcgcct
    16381 tcgttgtcca atacgttcac gttgtcgata gcgacttgtg gcagcggacg cggtacggaa
    16441 ccggttgcta cgatgacgtg tttggcttcg ataacggttt tctcgccttt gttatcgact
    16501 tcgatttggt aagcatcgcc atttttaccg gcaaaagaag cagtaccgaa caggctggtt
    16561 actttgtttt tttggaacag gaatttcacg ccgccggtca gcttggtcac gatggcatct
    16621 ttgcgctcaa tcattttggc cgcgtcgaat tttatgtcgc cgacagtgat accgtgttcg
    16681 gcaaactcgt gttgcgcggc gtggaaatgt tcgctggatt gcaacagggc tttggaagga
    16741 atacagccta cgttcaagca agtaccgccc aatgcagggg cattgcctgc tttgttaacc
    16801 cctgcgtcaa cgcaagcggt tttgaaaccc agttgcgcgg cacggatggc ggcaacgtat
    16861 ccgcccgggc ctgcaccaat cactactaca tcatattgag acatcgtgtc atcctttgct
    16921 tttgcagtat ctcaaaataa aaacgatact ttttgtttct tttttatttt gaatgactgc
    16981 attttgacgg ttttaagaaa aggccgtctg aaagtctata tctgccgatt cattcgaatc
    17041 aaatgtttca gacggcctca atcgttcgct gagcctattg tagcacgata ctacattacg
    17101 ctacatc
//
LOCUS       DAXHMX010000025        17011 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHMX010000025 DAXHMX010000000
VERSION     DAXHMX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17011
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.025"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(51..1220)
                     /gene="metK"
                     /locus_tag="ACFM4S_000635"
     CDS             complement(51..1220)
                     /gene="metK"
                     /locus_tag="ACFM4S_000635"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6050365.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1403..1513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1566..2462
                     /locus_tag="ACFM4S_000636"
     CDS             1566..2462
                     /locus_tag="ACFM4S_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6050366.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="ACFM4S_000637"
     CDS             complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="ACFM4S_000637"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6050367.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            3577..3702
                     /locus_tag="ACFM4S_000638"
     CDS             3577..3702
                     /locus_tag="ACFM4S_000638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050368.1"
                     /translation="MPLKTEKRILADFGTTAVSQKRNMPSEDRSDGIVAPYQSVP"
     gene            complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="ACFM4S_000639"
     CDS             complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="ACFM4S_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6050369.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4867..6819)
                     /locus_tag="ACFM4S_000640"
     CDS             complement(4867..6819)
                     /locus_tag="ACFM4S_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6050370.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGKGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7096..7719)
                     /locus_tag="ACFM4S_000641"
     CDS             complement(7096..7719)
                     /locus_tag="ACFM4S_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6050371.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7925..8554
                     /gene="yihA"
                     /locus_tag="ACFM4S_000642"
     CDS             7925..8554
                     /gene="yihA"
                     /locus_tag="ACFM4S_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6050372.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8675..11071)
                     /locus_tag="ACFM4S_000643"
     CDS             complement(8675..11071)
                     /locus_tag="ACFM4S_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6050373.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11223..12338
                     /gene="pilM"
                     /locus_tag="ACFM4S_000644"
     CDS             11223..12338
                     /gene="pilM"
                     /locus_tag="ACFM4S_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6050374.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12341..12940
                     /locus_tag="ACFM4S_000645"
     CDS             12341..12940
                     /locus_tag="ACFM4S_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6050375.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12941..13588
                     /locus_tag="ACFM4S_000646"
     CDS             12941..13588
                     /locus_tag="ACFM4S_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6050376.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLNAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13606..14151
                     /locus_tag="ACFM4S_000647"
     CDS             13606..14151
                     /locus_tag="ACFM4S_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6050377.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14170..16503
                     /gene="pilQ"
                     /locus_tag="ACFM4S_000648"
     CDS             14170..16503
                     /gene="pilQ"
                     /locus_tag="ACFM4S_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6050378.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAASAKQQAAAPAKQQ
                     AAAPAKQQAAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHT
                     LPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLP
                     KKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTL
                     SLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKALLQAEKDIADLGALY
                     SQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIE
                     KFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNS
                     GFGGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVL
                     TQNRKEAKIESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKI
                     NKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDI
                     PVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY"
BASE COUNT         4587 a         4829 c         3890 g         3705 t
ORIGIN      
        1 aaaatgccgt ctgaactgtt cagacggcat ttttgatttt caaaccggaa ttacagcccc
       61 gctgccgccc tcaatgcagc agctttgtcg gtgcgctccc aagtgaactc aggttcttcg
      121 cggccgaaat gtccgtaagc ggcggattta ctgtaaatcg ggcgcaagag atcgagcatt
      181 tggacgatgc ctttggggcg caggtcgaaa tgttcgcgaa ctaaggtaat cagtttttcc
      241 tcgctgattt tgcctgtgcc gaaagtgtcg atggaaatcg aagtcggttc ggcaacgccg
      301 atggcgtagg aaacttggat ttggcattgg gttgccaatc ctgcggcgac gatgtttttc
      361 gccacatagc ggcaggcgta ggcggcggaa cggtcaacct tggacgggtc tttgccggaa
      421 aacgcgccgc cgccgtgcgg agccgcgccg ccgtaggtat cgacgatgat tttacggccg
      481 gtcaaaccgc agtcgccttg cgggccgccg atgacgaagc ggccggtcgg gttgatcagg
      541 tatttggttt cgtcggtcag cagttcagac ggcaggacgg gcttgataat ctgctcgatg
      601 acggcgtttt tcagttcttc gtaagcgatg gacggatcgt gctgggtaga gaggacgacg
      661 gtatcaatgc gttttacctt accggtttcg ctgtcgtaaa ccacggtcaa ctgcgctttt
      721 gcgtcgggac gcagccacgg caggcggccg tctttacgca attcgctttg gcgttgcatc
      781 aggcggtggc tgtaatagat ggcaaacggc atcagggtcg gggtttcgtc gcaggcataa
      841 ccgaacatca aaccttggtc gcctgcgcct tggttcaagt cgataccttc gccttcgttt
      901 acgccttggg cgatgtcggg ggattgttgg tcgtagtaca cgccgaccgc gcagccgttg
      961 gcatcaaagc ccagctcgga ggagttgtag ccgatgcgtt tgatggtttc gcgtgcgact
     1021 ttgatgtagt ccacttgggc ggtggtggtg atttcgcctg ccaatacgca caagcctgtg
     1081 ttgaccaaag tttctgcggc gacacgcgct ttggggtctt gcgccaagat ggcatccaaa
     1141 atcgcatcgg atacttggtc ggcaactttg tccggatggc cttcggatac cgattcggaa
     1201 gtaaacagat attcgctcat tgagattttc cttgaaaaac aaaccatctt cttcagacgg
     1261 catgttgtat gaacataatg tcgacagcgg gaaatatagc aaaatttccc tattcatacc
     1321 attcagttga gaaatattcc catttgaatg gcactttgga atctctgccc gtacgtttct
     1381 tacaggcaaa aaaattcccg catcaagcgg gtttggattg ctctggtgag ccacatcggc
     1441 ttttcaaccg tccaccttac ttttcctttt gaaaagcagg ttggcatgga attcccaact
     1501 cttaatgcag cgcggatggt agcagaaaag cctttccgcc gcaatacttc cctttttcag
     1561 acggcatgtt tcgtttacaa ttcgggctgt ttcccccttt gcgaaccgcc atgcacatcc
     1621 tgttgaccgc cctgctcaaa tgcctctccc tgctgccact ttcctgtctg cacacgctgg
     1681 gaaaccggct cggacatctg gcgttttacc ttttaaagga agaccgcgcg cgcatcgtcg
     1741 ccaatatgcg tcaggcaggc atgaatcccg accccaaaac agtcaaagcc gtttttgcgg
     1801 aaacggcaaa aggcggtttg gaacttgccc ccgcgttttt cagaaaaccg gaagacatag
     1861 aaacaatgtt caaagcggta cacggctggg aacatgtgca gcaggctttg gacaaacacg
     1921 aagggctgct attcatcacg ccgcacatcg gcagctacga tttgggcgga cgctacatca
     1981 gccagcagct tccgttcccg ctgaccgcca tgtacaaacc gccgaaaatc aaagcgatag
     2041 acaaaatcat gcaggcgggc agggttcgcg gcaaaggaaa aaccgcgcct accagcatac
     2101 aaggggtcaa acaaatcatc aaagccctgc gttcgggcga agcaaccatc gtcctgcccg
     2161 accacgtccc ctcccctcaa gaaggcgggg aaggcgtatg ggtggatttc ttcggcaaac
     2221 ctgcctatac catgacgctg gcggcaaaat tggcacacgt caaaggcgtg aaaaccctgt
     2281 ttttctgctg cgaacgcctg cctggcggac aaggtttcga tttgcacatc cgccccgtcc
     2341 aaggggaatt gaacggcgac aaagcccatg atgccgccgt gttcaaccgc aatgccgaat
     2401 attggatacg ccgttttccg acgcagtatc tgtttatgta caaccgctac aaaatgccgt
     2461 aacgaaaata aaaatgccgt ctgaacaatt tcagacggca ttttgtcatc tgacgatttc
     2521 cgacagcggc cagcgcggac ggacattgaa cgcaccgacc tccctgccct tgcccaggtg
     2581 catcgcaccg gcaaaggcaa tcatggcacc gttgtccgtg cagtatgccg tcggcgggaa
     2641 aaacacgctg actttttcgg acggatgttt cggcttgcct tttggggtcg ggatttgcac
     2701 cgtcatattg ccgaaagttt cacggagttt gcggtttgca ccgaccccgc cggcgaccac
     2761 tacggttctg aaccctgtct gcaacagggc ttttttcact ttcgccgcca acacatcgac
     2821 taccgcatct tgaaacgcac ggcaaatatc gttgcgggtt tgctcgggaa tggtttccga
     2881 accgtttgcc tcacgcactt tttcaacggc agtcaaaaca gcggttttca aacctgaaaa
     2941 actcatctgc aaatcgtcgg aatgaatcat cgggcgcgga aaaacaaacg cttcgggcct
     3001 gcccgattcc gcaagttccg acagtttcgc accgcccgga tacggcaagc ccagcagttt
     3061 cgccgttttg tcgaatgcct cgcccgccgc atcatcgacg ctctcgccca aaagcgcgta
     3121 gtcgcctatg cccctgaccg ccataatctg cgtatgcccg cccgaaacca gcagcgcgac
     3181 aaaaggaaag tcgggttttt cctccgccaa cagcggcgac agcagatgtc cttccaaatg
     3241 atggacggga ataacaggct tgtccaacgc taaagccagc gcgttggcgt agctcgaacc
     3301 cgccagcagc gcaccgccca aaccgggccc ctgcgtaaag gcaaccgcgt caatgtcgcc
     3361 atacgatgcg cccgcctgcg ccaaacagcc ttcagtcaac ggaacaaggc ggcggatatg
     3421 gtcgcggctt gccaattccg gcacaacccc gccgtattcg gcgtgcattg ccatttgagt
     3481 gtgcaggcag tgcgcccgca atccacgttc cgtatcgtaa agcgcaacac ctgtttcgtc
     3541 gcaagaagac tcgattccta ataccaacat ggtctgatgc cgttaaaaac tgaaaaacgt
     3601 attttagcgg atttcggcac gactgccgta tcccaaaaac ggaacatgcc gtctgaagac
     3661 cgttcagacg gcatcgtcgc accgtatcaa agcgttccgt aagaatgcag cccgctcaaa
     3721 aacatattca cgccgataaa ggcaaatgcg gttacgaaca aaccgataat cgcccaccac
     3781 gccagcactt tgccgcgcca accggcaacc agccgcaagt gcagccaaac ggcgtaattg
     3841 agccagacga tgaacgccca cgtctctttc ggatcccaac tccaatagcg tccccaagca
     3901 tctgccgccc acagcgcacc caaaatggtg gcaatagtaa agaacagaaa gccgacggca
     3961 atcgccttat acatcacctc gtcgatcaat gccgacggcg gcagccacag ttttccgcct
     4021 tttccttccg cacgcaggga aaccagttcg gcaataccga gcatcgcgga aatgcaaaac
     4081 gcgccgtaac cgataaagtt tgccggaacg tggattttca tccaccagga ctggagcgcg
     4141 ggaatcagcg gctggatggt atgcgcctcg cgggacacgc tgtaccacaa gacaaatcca
     4201 accacgaccg ccataaagcc gaacacgaag ccgcccaatt tctgtatggc gaacttacct
     4261 tcataataaa gatacatcag cgcggtaatc accaaaaaca ggatgaacac ttcatacagg
     4321 ttggaaaccg gaatatgccc cgcatcggga cggagcagat agctttcgtg ccaacgtacc
     4381 agcagaccgg taaagcctgc tacggcagac acccatgcaa acacggttcc catacccaac
     4441 agcgtgttgg tcggcacatt ttttacgctt gccaaaaccg cgcccgaaat ataagcgaac
     4501 agggcgaaaa agacaaaggc gcactgccac atgatcgccg attggctgct gaggaaatac
     4561 cgcaacagga aaatctctgc cgatttgatg ttgccgccat acagaccgac ggcggcatag
     4621 gcaagcaata cgcttaaagg aacaaaccag cgcatcggtt tgaaaaacca acccaaaaac
     4681 acggcaatac cggcactcgc ccacaacatg accgtttcgt aaatgtccat atgcataccg
     4741 gaacgggtct gtacgaaaac cgtagccgca aaaaccagca cggcaaatac ccaatcccaa
     4801 agattcagat tgctgatcaa agacttctga atcagcagct cgtgttccgg aagggtttta
     4861 tagtgttcag tcatgattca agtccttgcc gagccgttgc agactctcga cgtgttttgg
     4921 aaattccttc tgcaaatccc gttcactgcg ggccgaagac atggcaaaac ggattttgcc
     4981 gtctgaaaac aatacccacg cccgtttttc gcgcacataa aacatcaata ccgtacccaa
     5041 taccaacagc accgagccga gatagaccaa aagcgcaccc ggggaacggg tcatctgcaa
     5101 acccgacgaa cgcacctcgg aaaacccatc aagttgcagc agcataggcg cgggatattc
     5161 ggtcaaaccc gtgtacgcat ccatactgtg cagcaggaaa cggttccgcg cttcatcctg
     5221 ctgccattcg ggcaagccgt accggcgtat ggtttcatcc aaagcagcgt tcatcacgcc
     5281 gtaaagcatt tcgtagaaat agccctgcat cttatcctgc tgctctttcg ggatattgga
     5341 cgtaataaat tcgtccaatc ccaaatagcc tttttgtgca aagatgttca gcgtgttttc
     5401 cgcagccagc atgaattgtt cgcggatttc ggcaggtgcg tctttggttg cgtcggcaac
     5461 cagacgtttg cgccccttcc cgtctttcag aaactcgcgc aacgccataa aggtgtccgc
     5521 tttcaactgc ttgtccaagg ggatacgcag ccagcggtat tgctgctgca agccgctgcg
     5581 cgtgccggta atccaaaaat aatcctgttc ctgcaaaacc ggcagcatat agtttttata
     5641 ttcgaccgcc tgccctgccg catcacggat acggtaaaca atggaagggc cgatattggt
     5701 gtatttttta ccttcctgag taacggcgcg gacatcgttc agcgtggatt tcaggctttt
     5761 ttcccgttcc gcgccctcgc tcatgtcctc cacattcata gaagtgaact gatcgaactc
     5821 aagacgatat ttgtgtttgc caatttccaa cggaaactgg tgtatggatg ttgccttcaa
     5881 cacgacaggc tcgcgcgaag catcacccaa attccacgcc ttgaatgtca aatccgaacc
     5941 gccgtcggca aaactcgcct gataaatcgt gatgccgtgc aaggtcaaag gatggttcac
     6001 gcggatggtg cgctcgagtt tctcaccggt tgccttgtcc gtcacttcaa tatcactggc
     6061 aaaatcgcgc ggcataccgg tattgtaaaa gtcgatatga aattttttca gtttgacttc
     6121 aaaaggcaag tcctgaacca atatcccgtt gtcggcattc aggaaaacca catccgcact
     6181 ctgcccctcg gaaatattga cgttgcccct aaatgagaga ttggacgcac ccaaaatact
     6241 ttcgggcttg aaatccttgg cataaaccgc ctgattgtcc ggaacaatcc gaccggtcag
     6301 catacccagt ttcaacagca ggttactgtc tatcaacccg cccaggcaaa tgacaatcaa
     6361 agcaacatgg gcaaagatat agccccattt gttcattgtg ccttttttgg cggcaatcag
     6421 aaccgacccg tcttcacggt taatggtttt tccctgaaaa ccttgtactt ccagataacg
     6481 tttggcaacc tcgggcgcaa tttttacatc caacagcgaa gaatggcgca tcgccgccag
     6541 agatttttct ttaacctttt cccgaaaaga cttcatttcg cgccagaacg gcggcacatt
     6601 gcgaatcagg cacaaactgg tagaaaccac caaaaacatc atgataacga caaaccatgc
     6661 cgaagcatag acgtcataca gtcccagaaa accaaaaatc tgcgcccaaa acgatccgaa
     6721 tttgaccaaa taatccgtct gcggctggtt ttgctgcaac accgtaccga taaccgatgc
     6781 aatacccagc agactgagca aagcgactgc aaagcgcatg gagctgaaaa aagcgaacca
     6841 cggacgggaa agaagtgggg gagatctacg ggatttactc attgtgtgtt tattccgcca
     6901 tcaggaatat gggaaagcag aattgggcaa acagaaaaca acgtcccgat tctactgtct
     6961 tgatgctttt tatttcaaga caatgaagac agcctgcatc gattccaacg gttgcgattg
     7021 aaaaaactta tcgcagaatt gcctgaagcc gtctgaaaac ttttcagacg gcctctaaaa
     7081 cagactattg cggaattaac gcaaaccttg gataaagttg gcgaccgctt tcaaatcttc
     7141 ttcagacata cggtttgcaa tatcttccat gatggtattt ttacgctgac cggacttgta
     7201 ggcattcatc tgttcaacaa tatatgcctg atgctgaccg cccaaacgcg gataagcctg
     7261 aatttcgctt ccgcctcccg gcatacccgc accgctcgga ccgtggcagg acatacacgc
     7321 cggcactttt ttatcgctca aaccgccgcg atagattttc gcacccaatt cgggattttc
     7381 cttaggattg gcttcaccgg atttgggctg ctgtttggca tagaatgcgg atacgttcaa
     7441 aatatcctga tcgctcaaat tcattaccac cggtttcatc acagctgccg aaccgtgggt
     7501 gcgtttaccg tcgcggatgc cgatagtttg atgatagatg taagcagtat gctgtgccgc
     7561 caaacgcgga tacatcgcaa tgccgctgtt accgtctgct gcatggcaag ccgcacaaac
     7621 cgttgcggca acctgtttgc ctttttccac gtctgctttg ggagaggcgg aaaccgcacc
     7681 ggcagccaaa acaaaggcca ataaagtcaa tcgtttcatg gagtgctcct gattacagca
     7741 ttggataacg caacaatgct ctttttatat tcaaatacgg gatttttgac ccgattaaaa
     7801 ccgatgattc tgtaaacgtg ttattctata ctaaatttac attaaattac cactgtgttt
     7861 cacataaaac caaccgcata tttttgctgt cggacaaacg gcggcggaaa acaaggatat
     7921 gcccatgaac ctttttcaaa acgccaaatt cttcacgacg gtcaaccacc ttaaagacct
     7981 gcccgacacc cctctcgaaa ttgcctttgt cggcaggagc aatgccggaa aatccagtgc
     8041 catcaatacc ctgaccaacc atgtccgtct tgcctacgtt tcaaaaacac ccggacggac
     8101 gcagcatatc aacttcttcg agctgcagaa cggcaatttt atggtcgatt tgcccggcta
     8161 cggttatgcc caagtccccg aagcagtacg cgcacattgg gtcaatctgc tcggcgacta
     8221 tctgcaacag cgcaaacagc ttatcgggct ggttttgatt atggatgccc gccatccttt
     8281 aaaagaactc gacatccgta tgctggactt tttccacacg accggcagac cggttcacat
     8341 cctgctgtca aaagccgaca aattatccaa aaacgaacag ataaaaaccc tgtcccaagt
     8401 caaaaaactg ctcaaacctt attccgacag gcaaaacatc agcgtacagc tgttttccag
     8461 cctgaaaaaa caaggtattg acgaggccaa ccgaactgtc ggaagctggt ttgacgcagc
     8521 agatgccgcc gcttcctctc cagaggaaaa ctgaccccaa ttatacggaa accgtatttc
     8581 ccccacttga ccgaccgcaa acatttaaaa aattgccact gccaaatcta aaatgccgtc
     8641 tgaaagactt ttcagacggc attttgcgga gtctttaaaa cagagaatcc aactgctgct
     8701 gtttggaacc agtattactc ggaagcaccg gcgtttcctg catatcttgg cggacttcgt
     8761 catccgccgc ctgccgtccg ccttctgccg cgcccccgtc atcttctttt gcccgtcggg
     8821 aaggttgcgg cgcaataccg ctgttgtcca gcgtcaagcc cggatcggtt accatacgtt
     8881 ccttcatata gtattcgcca ttgctgctga ccacaccttc aggcattttc atccccttgc
     8941 cctgctttcc tttcaacgca aaacgcatat agtccaccca aaccggcacc gcaatcgtac
     9001 cgccgtagcc gacacgcccc atactcttag gtttgtcgaa gccgatatat acggcagtaa
     9061 ccacatcagg gttaaaaccg acaaaccacg catccttatt gtcattggtc gtacccgttt
     9121 taccggcaat atccgttctt cccaacgcag ctgcccccct tgccgtacca acacggacca
     9181 catcctgcat aatcttatac ataatatagg cattgcgcgg atcgattgcc tgaggcgcat
     9241 tttgcccggc caccaaaggt tgcatttggg cgcgcaacct gccgtctctg tcataaatct
     9301 tatcgattac gtgcgaagaa accctatatc cgccgttcgc aaatacgcta tatgcctccg
     9361 ccactttcaa cggcgttgtc tcgcccgtac ctaaagccat agacaggctt gccggcagct
     9421 cggacgacct gaagccgaaa cgccggatat actgttgcgc gtaaccgaca ccgatagaca
     9481 tcaaaatacg gatggaaacc atattcttgg aagccgtcag agcctgtctc aaagtaatgt
     9541 agccggaata tctgccgtct gaatttttag gtgtccaaac cgaaccgttc ggccctttcc
     9601 ccggcaggga aatcggcgca tcgttaacca ctgtggacgc ggtcatcccc ttagataatg
     9661 ccgccgaata gacaaacggc ttaaaggtcg aacccggctg ccgcattgcc tgaacggcac
     9721 gattgaatgt tttgctgtga aaatcataac cgccgaccag cgcgcgcaca gctccggttt
     9781 ttgcatccag cgaaaccaaa gccccctgca gcaacggctc ttgaaccacc gcccaacgcc
     9841 cgccgttgtt tttgacacgg atgaccgcgc ccctgcggat acggtcctcc cccatttttt
     9901 cattattgac cgcgcgggcc gcaaaaccca aggcgcgcct gtcaagcgta acccgcctgc
     9961 cgccgggcag ctgtatgacg acatttttct ttttagtcac atccaacaca acggcgggaa
    10021 ccattttatc gacggtatag agtcccgaca gatactggct gacagtctcc tcgacatctt
    10081 cactcttact caaatcgata tagttttccg caccgcggta gctgctgccg cgatcgaaat
    10141 tccgtagagc cttgcgcaat gcctcggttg ccaccttctg atgatcggcg cggaccgtgg
    10201 tataaacctt aaaaccctgc gtataggcat cttcaccgta tttctcatac agttcctgac
    10261 gcaccatttc cgccacatat aaagcactct gatcgatttt ccgaacaaac cgctcgtaat
    10321 gcagttcctc attcaacgcc tgatcgcgct gttgcacggt aatcatcttc tcctcgagca
    10381 tattgttcaa aatatacttc tggcgcaact tggcacgttc cggattaaca atcggattat
    10441 aggcagacgg agccttgggc agtcccgcaa gcatggcggc ttccgccaaa gtcaaatctc
    10501 ggacattctt attgaaatag atttgcgcgg cagatgcaaa accataggcg cgctgaccga
    10561 ggtaaatctg attgaaatac aactcgagga ttttgtcttt gcttaaagac tgctcgattt
    10621 tataggcaag caacacctca ttgaatttgc gtgtgaacgt tttttcactg ctcaaataaa
    10681 aatttttcgc cacctgctgc gtaatcgtac tcgcacccga ctgcacgctg ccggacacga
    10741 cattgccgac ggcagcgcgg gcaacacccc aaacatccac cccccaatgc cggtaaaagc
    10801 gtttatcctc ggcggcgata accgcattcc gcaacacctc tgggaaatcg ccgatttttg
    10861 taaattcgcg ccgctgctcc ccatacatac cgatgacttc cccatccgcc gaataaatag
    10921 tcaacggcat tttaggctgg taatgctgca aagaatccaa agacggtagt ttcggatacg
    10981 ttaccaaaat agcaatggca accaaaccca ctccaaatac acaaaaccca aaaaccaaac
    11041 caaaacaagt cgttaaaatc tttttaatca tagctgaata ataatttacc attattggta
    11101 ttaaataaag taaaatagca accgatttct acaaagcacg gtttcaatgt gcaaagaaca
    11161 aggaatccat tacggatacc gaaacggtta ctcactgtac aaataaagca ggaactttca
    11221 tcatgcgctt gtttaaaagc ttgaaaaacc ctaaaaaaac agatgccaag ctccctaaaa
    11281 aatcttcggg actcaataac cgcgcggcaa tcggcatcga tatcgaccag cattccatca
    11341 aaatggtcca attgtcagga cgtagtttaa accaaattca attggaaaaa tacgtcattg
    11401 ccaaattacc aaagaatatc attcaaggca ataaagtcca aaattacgat caacttgtta
    11461 catatttgca acaagcctat gccaaactgg gtacttcgtg caaaaacatc atcgcgtccg
    11521 tcccgcaaaa tctggcaacc atcgaacaat tgacctacac agacaaagat gcagaattag
    11581 acctgcaggg gttcgtggag tcctccatct ccgaagtcag ctcgatatcg ctcgaagaag
    11641 ccaattacga ctatcaggtc ttgtcccaat cggccgccgg cgaagctgtg ttggccgtcg
    11701 catcgagaaa ggatgaaatc gaacccctga ttgacgcatt caacgccgcc ggtatgaaat
    11761 tatccgcgct tgatgtggac attttcggac aatacaacgc ctacgcgcta tggataaacc
    11821 atttcgcccc cgagcttgca gccgaaaaag tcgccatttt cggcgtatat gccgcacaga
    11881 cctacgcctt ggtcatccaa gacggaaaaa tcctatacaa acaggaaacc tccgtcagcg
    11941 aagaacagct caaccaactc atccagcgca cctatcaggt aacagaagaa aaagcggaag
    12001 aaatcatcaa ctccccgcaa aaaccttccg attaccaaga aagcgtggca aactatttca
    12061 accagcagat tacccaagaa atacaaaggg tcttgcagtt ttattacacc acgcagaccg
    12121 cagacgatat gaccgacatc aagcatatcc tgctgaccgg ggaagcggcg cgccaggaag
    12181 gcatcgccca aaccgtcgcc tcacaaacca atgcagatgt acaatgcgtc catcccgcgc
    12241 gttattttgc ggacaacctc aaaacagaca aacaacaatt cgaacttgat gcgccgacac
    12301 tgaccagggc gttcggtttg gcggtacggg gattataatt atgaacaatt taatcaaaat
    12361 caacctcctc ccctacaggg aagagatgaa caagcgcaaa cagcagcagt ttaaaacgct
    12421 gatgtacggt gccgtgctga cgggcgttgc cgccgttgcg gcaacctacc tgtttatcga
    12481 caatatgatc aataaccagt cggaaagaaa cacgctgctg gaaacctcca tcgcacactt
    12541 ggataccgag ctgtcggaaa tacaaaagct caaacaggaa aaagatgcct tcctgattaa
    12601 gaaaaacaaa atcgaggagc tccagctcaa acgcctccaa gccgcaaaaa tcctcgacag
    12661 cctgaatgag gccgtccccg gaagcaccta cctgacctcg ctggatgccg ttaccgccga
    12721 ctcttatcgg ctcagcggca ggacatccag cgacaaccgc gttgccgcca tgatgagggc
    12781 gatgcccaat accggcatat tcaagcaacc cgaattgtta agcatcaaga aaaacaattc
    12841 gcatcaagaa tttacccttc aggcaacatt acaacccatc gtaaaggcgg ccgaatccaa
    12901 agagaatccg gcttcgggaa acgcacagga ggcaaactga atggcttcta aatcatctaa
    12961 aaccaacttg gatctcaaca accttcacct gctcaacctt cctgccaggc tttttatcgc
    13021 cctgctggcc gttgccgccg tgctggggct cggttatgcc ggattgttca aaagccagat
    13081 ggaatccctt gaggaatacg aagcaaaaga aaccgaactg aaaaacacct acaaacagaa
    13141 aagtatcgac gcggccagcc tgaacaacct gagggacgaa cttgcctcaa tccgctctgc
    13201 cttcgatatc atgttgaaac agctgccgac agatgcagaa attcccaatc tggttcaaga
    13261 gcttcatcag gcaggttcga gcaacggtct gcgcttggac agcgttatgc cccaacctcc
    13321 cgtagatgac ggccccatca aaagattacc ctattccatt tccattaccg gaaattacga
    13381 acagatcagc caatttaccc gcgatgtcgg cagcctctcc cgaatcatta cccttgagtc
    13441 gctgaaaatc gcccaatctc cggaaaacgg cggcaatcct gacggcaaga gcagcatcct
    13501 gaacctcaac gccattgcca ccacctacca agcaaaatcc gtagaagagc ttgccgcaga
    13561 agcggcacaa aatgccgagc aaaaataact tacgttaggg aaaccatgaa acactatgcc
    13621 ttactcatca gctttctggc tctctccgcg tgttcccaag gttctgagga cctaaacgaa
    13681 tggatggcac aaacgcgacg cgaagccaaa gcagaaatca tacctttcca agcacctacc
    13741 ctgccggttg cgccggtata cagcccgccg cagcttacag gaccgaacgc attcgacttc
    13801 cgccgcatgg aaaccgacaa aaaaggggaa aatgcccccg acaccaagcg tattaaagaa
    13861 acgctggaaa aattcagttt ggaaaatatg cgttatgtcg gcattttgaa gtccggacag
    13921 aaagtctccg gcttcatcga ggctgaaggt tatgtctaca ctgtcggtgt cggcaactat
    13981 ttgggacaaa actacggtag aatcgaaagc attaccgacg acagcatcgt cctgaacgag
    14041 ctaatagaag acagcacggg caactgggtt tcccgtaaag cagaactgct gttgaattct
    14101 tccgacaaaa acaccgaaca agcggcagca cctgccgcag aacaaaatta agaagaggat
    14161 tactccatta tgaataccaa actgacaaaa atcatttccg gtctctttgt cgcaaccgcc
    14221 gcctttcaga cagcatcggc aggaaacatt acagacatca aagtttcctc cctgcccaac
    14281 aaacagaaaa tcgtcaaagt cagctttgac aaagagattg tcaacccgac cggcttcgta
    14341 acctcctcac cggcccgcat cgccttggac tttgaacaaa ccggcatttc catggatcaa
    14401 caggtactcg aatatgccga tcctctgttg agcaaaatca gtgccgcaca aaacagcagc
    14461 cgtgcgcgtc tggttctgaa tctgaacaaa ccgggccaat acaataccga agtacgcggg
    14521 aacaaagttt ggatattcat taacgaatcg gacgataccg tgtccgcccc cgcacgcccc
    14581 gccgtaaaag ccgcgcctgc cgcaccggca aaacaacagg ctgccgcacc gtctaccaag
    14641 tccgcagtat ccgtatccga accctttacc ccggcaaaac aacaggctgc cgcaccgttt
    14701 accgagtccg tagtatccgt atccgcaccg ttcagcccgg caaaacaaca ggcggcggca
    14761 tcagcaaaac aacaggcggc ggcatcagca aaacaacagg cggcagcacc agcaaaacaa
    14821 caggcggcag caccagcaaa acaacaggcg gcagcaccag caaaacaaac caatatcgat
    14881 ttccgcaaag acggcaaaaa tgccggcatt atcgaattgg ctgcattggg ctttgccggg
    14941 cagcccgaca tcagccaaca gcacgaccac atcatcgtta cgctgaaaaa ccataccctg
    15001 ccgaccacgc tccaacgcag tttggatgtg gcagacttta aaacaccggt tcaaaaggtt
    15061 acgctgaaac gcctcaataa cgacacccag ctgattatca caacagccgg caactgggaa
    15121 ctcgtcaaca aatccgccgc gcccggatac tttaccttcc aagtcctgcc gaaaaaacaa
    15181 aacctcgagt caggcggcgt gaacaatgcg cccaaaacct tcacaggccg gaaaatctcc
    15241 cttgacttcc aagatgtcga aatccgcacc atcctgcaga ttttggcaaa agaatccgga
    15301 atgaacattg ttgccagcga ctccgtcaac ggcaaaatga ccctctccct caaggatgtg
    15361 ccttgggatc aggctttgga tttggttatg caggcgcgca acctcgatat gcgccagcaa
    15421 gggaatatcg tcaacatcgc gccccgcgac gagctgcttg ccaaagacaa agccctctta
    15481 caggcagaaa aagacattgc cgatttgggt gcgctgtatt cccaaaactt ccagttgaaa
    15541 tacaaaaatg tggaagaatt ccgcagcatc ctgcgtttgg acaatgccga cacaaccgga
    15601 aaccgcaata cgcttgtcag cggcaggggc agcgtgctga tcgatcccgc caccaatacc
    15661 ctgattgtta ccgatacccg cagcgtcatc gaaaaattcc gcaaactgat tgacgaattg
    15721 gacgtacccg cgcaacaagt gatgattgag gcgcgtatcg tcgaagcggc agacggcttc
    15781 tcgcgcgatt tgggcgttaa attcggcgcg acaggcaaga aaaagctgaa aaatgataca
    15841 agcgcattcg gctggggggt aaactccggc ttcggcggcg acgataaatg gggggccgaa
    15901 accaaaatca acctgccgat taccgctgcc gcaaacagca tttcgctggt gcgcgcgatt
    15961 tcctccggtg ccttgaattt ggaattgtcc gcatccgaat cgctttcaaa aaccaaaacg
    16021 cttgccaatc cgcgcgtgct gacccaaaac cgcaaagagg ccaaaatcga atccggttac
    16081 gaaattcctt tcaccgtaac ctcaatcgcg aacggcggca gcagcacgaa cacggaactc
    16141 aaaaaagccg tcttggggct gaccgttacg ccgaacatca cgcccgacgg ccaaatcatt
    16201 atgaccgtca aaatcaacaa ggactcgcct gcgcaatgtg cctccggtaa tcagacgatc
    16261 ctgtgtattt cgaccaaaaa cctgaatacg caggctatgg ttgaaaacgg cggcacattg
    16321 attgtcggcg gtatttatga agaagacaac ggcaatacgc tgaccaaagt ccccctgttg
    16381 ggcgacatcc ccgttatcgg caacctcttt aaaacacgcg ggaaaaaaac cgaccgccgc
    16441 gaactgctga ttttcattac cccgaggatt atgggtacgg ccggcaacag cctgcgctat
    16501 tgatgcgtca aaataagggc atatgtttta cggcatatgc cctttcttta tgctttttgc
    16561 cgcgaccgaa atgccgtcat tcccgcgcag gcgggaatcc agtccgttca gtttcggtca
    16621 tttctgataa attcctgttg cttttcattt ctggattccc acttttgtgg gaatgacggc
    16681 ggaaggggta aatcctcgca atccaaagcc tcgtcatttc cacaaaaaac agcaacccgg
    16741 aacagcaact taaaaccccg tcattcccgc gcaggcagga atccaacttg tccggttccg
    16801 gttgtttttc gtctcgtaac ttttgagccg tcattcccgc gcaggcggga atctaggtct
    16861 gtcggttcag gaacttatcg gataaaacgg tttctccaac cctgcgttct agattcccac
    16921 tttcgtggga atgacgggat atgggtttcc gtgcggacgt gttcggattt ccgcctgcgc
    16981 gggaatgacg gcgacagatg cccaacggtc t
//
LOCUS       DAXHMX010000026        16946 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHMX010000026 DAXHMX010000000
VERSION     DAXHMX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.026"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(93..932)
                     /locus_tag="ACFM4S_000649"
     CDS             complement(93..932)
                     /locus_tag="ACFM4S_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6050379.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADGAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="ACFM4S_000650"
     tRNA            1217..1292
                     /locus_tag="ACFM4S_000650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="ACFM4S_000651"
     CDS             complement(1357..2550)
                     /locus_tag="ACFM4S_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6050380.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVRRAETARAATPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="ACFM4S_000652"
     CDS             complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="ACFM4S_000652"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6050381.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAAAAQSPELKKRW
                     YAVEFLSTLSGEMLVTMIYHKRLDAEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTRKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3806..4906
                     /gene="aroC"
                     /locus_tag="ACFM4S_000653"
     CDS             3806..4906
                     /gene="aroC"
                     /locus_tag="ACFM4S_000653"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6050382.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIRVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRSAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4998..5423
                     /locus_tag="ACFM4S_000654"
     CDS             4998..5423
                     /locus_tag="ACFM4S_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6050383.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETLSVEAEAAESSAAE"
     gene            complement(5497..7482)
                     /gene="parE"
                     /locus_tag="ACFM4S_000655"
     CDS             complement(5497..7482)
                     /gene="parE"
                     /locus_tag="ACFM4S_000655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6050384.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVAGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7549..8097)
                     /locus_tag="ACFM4S_000656"
     CDS             complement(7549..8097)
                     /locus_tag="ACFM4S_000656"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6050385.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINNRDEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQSSDNR"
     gene            8239..9534
                     /gene="serS"
                     /locus_tag="ACFM4S_000657"
     CDS             8239..9534
                     /gene="serS"
                     /locus_tag="ACFM4S_000657"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6050386.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYKFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9612..10610)
                     /locus_tag="ACFM4S_000658"
     CDS             complement(9612..10610)
                     /locus_tag="ACFM4S_000658"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6050387.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10735..11811)
                     /gene="prfA"
                     /locus_tag="ACFM4S_000659"
     CDS             complement(10735..11811)
                     /gene="prfA"
                     /locus_tag="ACFM4S_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6050388.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKINTLDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITNALIAEHQAELLAAMGD"
     gene            complement(11901..12671)
                     /locus_tag="ACFM4S_000660"
     CDS             complement(11901..12671)
                     /locus_tag="ACFM4S_000660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6050389.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATRPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(12674..13666)
                     /locus_tag="ACFM4S_000661"
     CDS             complement(12674..13666)
                     /locus_tag="ACFM4S_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6050390.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGADEVKHELQRLGLV"
     gene            complement(13733..14416)
                     /locus_tag="ACFM4S_000662"
     CDS             complement(13733..14416)
                     /locus_tag="ACFM4S_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6050391.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14605..15939)
                     /gene="glmM"
                     /locus_tag="ACFM4S_000663"
     CDS             complement(14605..15939)
                     /gene="glmM"
                     /locus_tag="ACFM4S_000663"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6050392.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLVGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLAAVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16069..16920)
                     /gene="folP"
                     /locus_tag="ACFM4S_000664"
     CDS             complement(16069..16920)
                     /gene="folP"
                     /locus_tag="ACFM4S_000664"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6050393.1"
                     /translation="MARHGWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAKTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSSEEEWARVEPVLVEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMQGLPETMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMIGELTGEADAAARVHGSVAAAVAAVARGAQIVRVHDVKATADALKVW
                     EALGINL"
BASE COUNT         3757 a         4742 c         4547 g         3900 t
ORIGIN      
        1 tcctgatttt tgttaattca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttaaaatt ttgcaccgga ttggtttgcc
      121 atataatcaa cagccgcttt gacttcgtca tcgctcaaac cggcattgcc gcctttggca
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac catcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga cgggctggat acgggtctgc
      781 gttgccgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tgttgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaataat cctttgaaaa taatggttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttacgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gagaccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgatcgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacagtg aatttgagct gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgctcagg ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aagccttggt aggcgatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgttgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 gggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccaacag tggcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcgccttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcgggaaac cagcggtgca ggaagtccgc
     2221 cccgactttg agtgcgcccg agccgcccaa ggtttgtacg gtagcgatgc gtccttgcgc
     2281 gagcgcaggg ttgtctttgc cgaacaataa atgctgcacc gcgctgcggt aagtgtccaa
     2341 gccttccatc ggcaggtagg gcgacggcgc aggcgtggcg gcgcgggcgg tttcggcacg
     2401 gcgcacggat tccaacacgg gcattttgcc ttcgtcgtcg aaataaatgc ctatgctcaa
     2461 attgactttt tcagggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcgggtc
     2521 gcccggatag tattcgatgt gtcggtacat agtccttacc ttttactttt tcaaaggatt
     2581 ttctttttca acaatacacc actttcgata tggtgcgtaa acgggaattg gtcgaacagg
     2641 gcggcgcgtt cgaccgcatg ggtttccacc aaggtgtcca aattggcacg cagcgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgttttctgg tataggcttc ggtaaattct
     2881 tcagcagaca gacgggcgat tttgatgttg ccgatgcggt tggcttcgat attccattgc
     2941 gccgcgctga tggaggtttt ggagatttcg gttgccaaaa cacgttcaaa cttttcagat
     3001 aaaggaagcg taaagttgcc gtttccgcaa tacagttcga gcaaatcccc atccaatcct
     3061 tctgctgcac cgcaggccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccaataac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcag catcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtggacagaa attcgacggc ataccagcgt
     3361 tttttgagtt cgggggattg cgcggcggcg gcgatcagct cgggcatgag gcggttgaca
     3421 gcctcggaag ctgcttcaaa acggtcgcag cgtatcatgc ttgcgccgct ggctttctgc
     3481 cctttttcaa acatggcata aaacatttcc ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt aatgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggta gtctttttta ccttgaagct gctgcgtgta gtcattcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattt gcctcgccgc acgcaaactc
     3721 tttgactgcc gccccttcaa tgacggacgg gcttttgtgc taaaatccgc catctttcca
     3781 cactataccg ataaagggaa aaatcatggc aggcaacact ttcggacaac tcttcaccgt
     3841 taccaccttc ggcgaaagcc acggcgcggg tttgggctgt atcatcgacg gctgcccgcc
     3901 cggcttggaa ttaagcgaag cggatatcca atttgacctc gaccgacgca aacccggcac
     3961 cagccgccac gttacccaac gccgcgaagc cgaccaagtc gaaatcctct ccggcgtatt
     4021 cgaaggcaaa accaccggca cgcccatcgc cctcttaatc cgcaataccg accagcgcag
     4081 caaagactac ggcaacatcg ccaccagctt ccgccccggc cacgccgact atacctattg
     4141 gcacaaatac ggcacgcgcg actaccgcgg cggcggcagg agctccgccc gtgaaaccgc
     4201 cgcccgcgtt gctgccggag ccgttgccaa aaaatggttg aaagaaaaat tcggcacgga
     4261 aatcaccgcc tacgttaccc aagtcggcga aaaagaaatc cggtttgaag gctgcgaaca
     4321 catttcccaa aatccttttt ttgccgccaa ccatagccaa attgccgagc tggaaaacta
     4381 tatggacggc gtgcgcaaat ccttggattc cgtcggcgcg aagctgcata tcgaagcagc
     4441 caatgtccct gtcggcttgg gcgaacctgt ttttgaccgc ctcgatgccg aaatcgccta
     4501 cgcgatgatg ggcatcaacg ccgtcaaagg cgtggaaatc ggcgcaggtt ttgacagcgt
     4561 aacgcaacgc ggcagcgaac acggcgacga actgaccccg caaggcttcc tgtccaacca
     4621 ctcaggcggc atcctcggcg gcatcagcac cgggcaagac atccgcgtca atatcgccat
     4681 caaacccacc agctccatcg ccaccccgcg ccgcagtatc gacatcaacg gcaaccccat
     4741 cgaactcgcc acgcacggca ggcacgaccc ctgcgtcgga ctgcgctccg cgccgatcgc
     4801 cgaagccatg ctcgcgttag tcctcatcga ccacgccctg cgccatcgcg cgcaaaatgc
     4861 cgacgttcag gttaatacgc ccgacattac cctttcaaac aaataaaaat ttagccaaaa
     4921 cacagacttt ataatagaat atcgagcatt gccgtgcagc cttgacgcac gggtttgttt
     4981 agaggaatac aaccgaaatg acacaagaaa ccgctttggg cgcggcacta aaatccgccg
     5041 tccaaactat gagcaaaaag aaacagaccg aaatgatcgc cgaccacatc tacggcaaat
     5101 acgatgtgtt caaacgcttc aaaccgttgg cgctcggcat cgatcaggat ttgattgccg
     5161 ctttgccgca atacgatgcc gcactgattg cacgcgtcct cgccaaccac tgccgccgtc
     5221 cgcgctatct gaaagccttg gcgcgcggcg gcaaacgttt cgatttgaac aaccgtttca
     5281 aaggcgaagt tacccccgaa gaacaggcga tcgcgcaaaa ccatcctttt gtgcagcagg
     5341 ctttgcaaca gcagtccgcc caagctgccg ccgaaacgct gtctgttgaa gccgaagcag
     5401 ccgaatcttc cgcagcagaa taaatcccca aacgaaatgc cgtctgaaaa ccgatttggt
     5461 ttcagacggc attttttcgt atgcggcaat cacggttcaa atatcgagtt ccgccgtatc
     5521 gccttcgcgt tccatccagg cacggcgggc ggcggcttcg cctttgccca tcagtttgac
     5581 gaagatgtcg cgcgtctcgt catccgcgcc ttctgggatt tgtacctgca acaggcggcg
     5641 ggtgtcgggg tgcatggtag tatctttgag ctggtctggg ttcatctcac ccaagccttt
     5701 gaaacggctg atggaataag cagtttcttt aacgccttct ttttgcagcc gctctaaaat
     5761 gccgtcaagt tcgttttggt cgagggcgta gaatttgcgg gcgggtttgc ttttaccttg
     5821 tgcgttgaca tctacgcgga acagcggcgg ctgggcgacg tagatgtgtc cgtcagcgac
     5881 cagtttcggg aagtggcggt agaacagggt cagcagcaaa acttgaatat gcgagccgtc
     5941 cacgtcggca tcggacagga tggcgatttt gccgtaacgc aggccgctta aatcgggatg
     6001 gtcgttgatg ccgtgcggat cgacgccgat ggcaactgaa atgtcgtgga tttcggcgtt
     6061 gccgaagagc tggtcggggt ggacttcaaa gctgttgagc actttgccgc gcaggggcag
     6121 gatggcctgg gtggctttgt cgcgggcgag tttggctgag ccgccggcgg aatcgccttc
     6181 gacaaggaag agttcgtttt cgcggatgtc ttcgctttcg cagtcggtca gcttgccggg
     6241 caggacggcg acgccgctgc cttttttctt ttcgattttc ttaaccgaac gcatccgcgc
     6301 ctgcgcctga cggatggcga gttcggcaat ttttttaccg aagtccacgt tttggttcag
     6361 ccacaattcc aaagggtcgc ccgctacagc ggcgacaagt ttcagcgcgt cgcggttggt
     6421 cagcttgtct ttggtctgac cttggaactg cgggtcgagg acacgggcag agaggacaaa
     6481 ggcggttttg ccgaacacgt cgtcgctttg tactttcacg ccgcgcggca agaggttgtg
     6541 cagattgatg aaattgttga cggcgttgaa cacggcttgt ttcaagcctg cttcatgcgt
     6601 gccgcccaaa ggggtgggga tgaggttgac atagctttcg ttggcacacg agccttcttc
     6661 cagccaagtc agggcaaacg ccgctccttc gccgatgctg aaatcgccgt tgtgttcgtc
     6721 tgaaatgtag ttttcgcaag agaacagcgg cacagcttct tgcgcgtcgg caatcaggtc
     6781 ggtcagatag cttttcaggc cgtcggggta gtgccaggtt tgggtgtgcg cttcgtcttc
     6841 gcctttgacc ggacgggtca gggaaacgcg cacacccggc agcagcacgg ctttggcacg
     6901 cagcaggcgt tcgagttcgg gaatgctgta attcgggctt tcaaaatatt tgccgtccgg
     6961 ccagacgcgc acttcggtgc cgctgtcttt gacggcgcat ttgcccactt gtgccaacgg
     7021 ttcgaccacg tcgccgccgg caaacacgat acggtggatt ttgccttcac gtttgaccgt
     7081 tacctcaagg cgggtggaga gggcgttggt gacggatacg cccacgccgt gcagtccgcc
     7141 tgaaaaggca tacgcgctgc cgccgtcttt tttgttgaac ttgccgcccg cgtgcagacg
     7201 ggtgaacacg agttcgacta cgggtacgcc ttcttcaggg tgcaggccga cgggaatacc
     7261 gcggccgtta tcacggacgg aaagcgaacc gtcttcatga atttgcacgt cgattgcagt
     7321 cgcgaaacca cccaacgcct cgtccgccgc attatcaatc acttcctgac agatgtgggt
     7381 cgggctgtcg gtgcgggtgt acatgccggg acgttctttg accggctcca agcctttgag
     7441 gacggtgatg ctggattcgc tgtattggtt gtttttagcc ataggaataa tctgaaagta
     7501 agaaaaacaa cgctttcaga cggcctgaaa gcgttgcgtt ccgttgtttt agcggttgtc
     7561 ggaagattgg cgggcggcaa agtcttcata actttccata ccgcgcagga agcgggaaga
     7621 gagttctttc aacgccccca aataaacgtc gcgtttgaaa tcaatcactt cgtcaacggg
     7681 cgcccaatat tgatgccaac gccagccgtc aaattcgggg tggcgggtgg cgcgcaggtt
     7741 gacatcgcaa tctcggccgg tcaggcgcag gagataccaa atctgcttct gcccgcgata
     7801 ggatccgcgc cattcgcggc gtacccagtt gttcggcacg tcataacgca gccagtcgcg
     7861 cgtgcggccg ataattttga cgtgttgcgg caaaagcccg acttcttcgt acaactcgcg
     7921 gtacatggcg gtttcggggc tttcgcccgg cttgatgccg ccttgcggaa actgccaaga
     7981 atgttcgcgc acgcgcttgc cccaaaagac ttcgtcgcgg ttgttgatta agatgatacc
     8041 gacattgggg cgatagcctt ccctgtccaa cacggtgtcg ccctccgtta aattcaatct
     8101 tgggattttc ccacaaatcg gacggttttg acaaatcgga cgatagggtt caggcatgtc
     8161 gaaacacaga cggattcggg aaaatatctt aaatttggtt tacaataata cgtttcaaat
     8221 taattcggga atcagaccat gttagatatc caattgctcc gcagcaacac cgccgctgtg
     8281 gcggaacgct tggcggcacg cggttacaag tttgatgccg cgcgtttcaa tgcgctggaa
     8341 gagcagcgaa aagccgtcca ggtgaaaacc gaagagctgc aagcctcgcg caacagcatt
     8401 tccaaacaaa tcggcgcact gaaaggtcag ggcaaacatg aagaggcgca ggcggccatg
     8461 gatcaagtcg cccaaatcaa aaccgatttg gaacaggcag ccgccgattt ggaagccgtg
     8521 caaaaggaac tggacgcatg gctgttgagc attccgaacc tgccgcatga ggacgtgcct
     8581 ttcggtaagg atgaaaccga aaacgtcgaa gtccgcaaag tcggcacgcc acgcgaattt
     8641 gactttgaaa tcaaagacca tgtcgatttg ggcgaacctt tgggtttgga tttcgaaggt
     8701 ggcgcgaaac tttccggcgc acgatttacc gtgatgcgcg gacaaatcgc ccgcctgcac
     8761 cgcgcgctgg cgcaattcat gctcgatacg cacacgctga aacacggcta caccgagcat
     8821 tacacaccct atatcgtgga cgacaccacc ttgcaaggca cgggtcagct gccgaagttt
     8881 gcagaagatt tgttccacgt tacccgtggc ggcgacgaga gcaaaaccac gcaatatctg
     8941 attccgacag ctgaagttac cctgaccaat accgttgccg gcagcattat cccgtccgaa
     9001 caactgccgc tgaagctgac cgcgcattcg ccctgtttcc gcagcgaggc gggttcgtac
     9061 ggcaaagaca cgcgcggtct gattcgccag caccagttcg acaaagtgga aatggttcaa
     9121 atcgttcatc ccgaaaaatc atacgaaacg ctggaagaaa tggtcggcca tgccgaaaac
     9181 atcctgaagg ctttggaact gccctaccgc gtgattaccc tgtgtaccgg cgacatgggc
     9241 ttcggcgcaa ccaaaacgta tgacttggaa gtttgggtac cggcgcaaaa cacctaccgc
     9301 gaaatctcaa gctgctccaa ctgcgaagat ttccaagccc gccgcatgaa ggcgcgtttc
     9361 aaagacgaaa acggcaaaaa ccgcttggta catactttga acggctccgg cttggctgtc
     9421 ggcagaacgc tggtcgccgt attggaaaac catcaaaacg ccgacggcag catcaacatc
     9481 cctgccgcac tgcaaccgta tatgggcggc gttgccaagt tggaagtgaa ataagtttgc
     9541 aagctgcctg aacgtcaaat gccgtctgaa acctggttca gacggcattt cctttaaact
     9601 tttaaaacac atcaaccgtc ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc
     9661 tcgcggatat tggacagcgt aacttccgaa atattaccca acgcctcttc cgtcaaaaat
     9721 gcctgatggc cggtaaacag cacattatga caagacgaca ggcggcggaa cacgtcgtcg
     9781 gtaatcacat cgttggattt gtcttcaaaa aacaattcgc gttcgttctc gtacacgtcc
     9841 atacccaatg cgccgatttt ccggcgtttc aacgcctcaa tcgcggcaga actgtcaatc
     9901 agaccgccac ggctggtgtt gataatcatc acgccgtctt tcattttgtc gaacgccgct
     9961 tcgttcagca tatagtggtt ttccggcgtg gaggggcaat gcagcgtgat gatgtccgac
    10021 cgggcataca gctcgttcaa ctccacatac ctgccgccga ttttttccgc ttcggggttg
    10081 caaaacggat cgtaagccag caggttcatg ccgaaaccct ttaaaatccg catggttgca
    10141 ataccgattt tccccgtgcc gataacgccc gccgttttgc cgtacatatt gaaaccggtc
    10201 agaccttcca gcgaaaaatt cgcatcgcgg gtacgctgat aggctttgtg gatacggcgg
    10261 ttcaacgtca gcatcagacc gaccgtatgt tccgcaaccg attcgggcga ataggcaggc
    10321 acgcgcacga ctttcaagcc caactcttca gccgccttta aatccacatt attgaagccg
    10381 gcacaacgca acgccacagt tttcacgcca atttgcgcca atttttccaa cacgggccgg
    10441 ctgccgtcgt cgtttacaaa aatacagacc gcttccgcgc cttccgccat tttcgccgtt
    10501 ttcgcatcca gcataaaatc aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg
    10561 gtaaaatgtt cgcggtcata gcttttcgta ccgtaaatcg caatcttcat caatatgtcc
    10621 agttgtcgcc tatggttgag aaacggcata atacactgaa ttcaaacaaa tcagtagaat
    10681 atggcagatt aaaattgatt gcatacacgg catttccatt tcaaaacaca aaactcaatc
    10741 gcccattgcc gccagaagct ccgcctgatg ctcggcaatc agggcattgg tgatttctgc
    10801 caagtcgccg tccatcacaa aatccagctt gtgcagggta aggttgatgc ggtggtcggt
    10861 taccctgcct tggggatagt tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat
    10921 gagggatttg cgctcggcgg cttctttggc ttgggcttcg cgtttttgcg cgtcgttcag
    10981 gcgggcggcg aggactttca ttgcctgcgc tttgttggca tgttggctgc ggccgtcttg
    11041 gcattcgacc accatgccgg tgggcaggtg ggtgatgcgg acggcggagt cggttttgtt
    11101 gatgtgctga ccgcccgcgc cggatgcgcg gaaggtgtcg atgcgcaggt cggctgggtt
    11161 cagttcgatg tcttccagtt cgtccgcttc gggcatgacg gcaacggtgc aggcggaggt
    11221 gtggatgcgg ccttggcttt cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc
    11281 aaatttcaaa cggctgtacg ccccgagtcc gacaatacgg gcgatgactt ctttatagcc
    11341 gcccaattcg ctttcgttgg cggacacgat ttcaacctgc caacggttgc gctcggcgta
    11401 gcggctgtac atacgcagca aatcgccggc aaacagcgcg gcttcgtcgc cgcccgttcc
    11461 ggcgcgtatt tcgatgaaga tgtttttgtc gtcgtcggca tctttgggca gcagcagttt
    11521 ttgcagttcg gtatcgagcg tgttgatttt ggcttttgcc gcttcgattt cttcggcggc
    11581 aaaatctttc atttcggggt cggacaacat ttcttcggca tccgccaagt cgctttgggc
    11641 aagccgatag ttttggaaca cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag
    11701 cttgcggtag ttgtccatat ctgccgtggc ttcgggttgc cctaaaaggt gggtaacttc
    11761 ttccaaccgg tcgccgagtt gttgcagttt ttctaagata gacggcttca taattcttcc
    11821 ataacaaacg ccgcctgaat ttcagacggc atcaaaacgc ggtcattata atatattttc
    11881 tgaatattca aaaagataat ctagggacgg agcaggctgt cggcattgcg ttccgttatc
    11941 gcggcgatgt ccgccacgcc gacaccgcgt atctgcgcgg caatttcggc aatccaaaga
    12001 atattggcag gcgtattaat ctcttttttc agcataaacg ggctgtcggt ttccaatacg
    12061 aaatccccgt cgttcaaggc tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc
    12121 aacaacgaac cgatgccgat tttgaaaccc agtttcgtca acacgcgcgc ttcttccgcg
    12181 ctgcctgaga aggcgtgaac gatgccgcct tgggcaaagc ctgtctgttt gacggcggcg
    12241 gcgatgtcgg cggtggcttt gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg
    12301 atggcaagct ggcggacgaa aacttggatt tgccgttcgc gctgcggcgg cgtttgggtt
    12361 ttgtcgtaaa aatccaaacc gatttcgccg acccatgcct gcggataacg tgccaacatc
    12421 gcttccaggc ggacgcaatc acgctcggca atgccgtctg aaaaccaagg atggatgccg
    12481 agcgcaatac ggatttgacc gtgttcggac ggcatttccg acagttccgc cacgctttgc
    12541 caatcctgcg ggcgcgtcgc gggaacgata aaccgccaca cacccgcctg tcgcgcttcg
    12601 gcaagcacct gcgccagatt gccgcgcaaa acagaatcgg caagatggca atgtgtgtcg
    12661 gtcagcctca tcttcacacc agccccaacc gctgcaactc gtgtttcacc tcgtccgcgc
    12721 cccatccttc agaaacggca agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc
    12781 cgccgttgat gatgcctgag ttgcggaacg cgttgccttg cgcgtaaacg gcagcggttc
    12841 tgccttgtcg gacttggctg atgttgagca gcagtttgcc ttgctgcgta aagtcttgca
    12901 cggcgcggat gaaaccttcg tctgcgggcg tgttgccgtg tccgtaactt tgcaggataa
    12961 gggcgtgggc gggaagctgt ccgagtccgt cggcaagttc ttggacggca aagccgggga
    13021 taagcgtgcg gacggcgatt tttgcctgcg ggtcgggata acggattttg aggccgtctg
    13081 aaacatctgc cgcgtcctga ttcgggatgc ggacattgtg ccagccctgg gtttcgtccc
    13141 actccgccag tgcgccgaaa tgcggattgt cgaaaccggc ggcggtttcg gtgctgactt
    13201 tgctgctgcc gacggcggga taaagtttgc cgtcaaaggc aataagcgtc tgcttcaatc
    13261 cgagtgggaa tgcggcaacg gcagtcgcca gattgcgcgg cgcgtcgctg cccacggcgt
    13321 tgtacggcca ttgggagccg gtcaggacga ttggcttgtc caaaccctgc agggcgaggg
    13381 cgaacagatt ggcggtatag gcgagcgtgt ccgtgccgtg cagcaccaaa acgccgtcgt
    13441 actgcggcag cgcggcggca atgatgtcca gccaagcgca ccagttttgc agttggacgg
    13501 cggaagaatc aatcagcgtc ccacaaacgt gccaatcgaa atccatgccg tttgaaaacg
    13561 gcgcgagtgc ctgcccgacc agcgcggtat cggggcgcag gccttcgctg ctttgcgtca
    13621 tgccgatggt gccgccggta taaaggacaa aaattttctg tttcatggct catcctgaaa
    13681 atgcaaagac cgcctgaaac gcgaaatgcg tccggacggt cgaaaatctg atttactgct
    13741 gcttctgtgc cgcttttttc gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca
    13801 attgtgcgcg gtaaagctga taatgtcggc gtttgcgcca ccagaaccac gccagcgccg
    13861 ccgccagcac gcccaatgcg atgaagatgc cggattgcag gctgtgcatt ttcgccatca
    13921 gccaatcgat gttgtgcgcg ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca
    13981 gcgcggcaag cccgtccata atcagaaagc gcagatacga tactttgcgg ctgatgccgg
    14041 cggtaacgaa aacggcagtc cgcaaacccg gcaggaagcg ggcgacaaac agaacccagt
    14101 tgccgtattt gtcgaatttt tcctgaacct gtgcgtaacg tttcggcgtc atgatgcgcg
    14161 caatcggttt gaacttgagg attttctgcc cccagatgcg tccggcggcg aacatgatgc
    14221 cgtccccgac caatacgccg agcataccga ctgcaaacat aatatgcgga ttggtataac
    14281 ccatacccga aatcacgccg cctgttacca aggtcaaatc ctcgggaatc ggcacgccga
    14341 aaccgcagat gaccaaaacg aaaaacacgg ccgcgtagcc gtattcgaca aaaaaggctt
    14401 ccaaaagggc aaacatagcg gatattccat tgtcggagat aaaaagtcag aacaagccga
    14461 aacattttct gcgcgaaacg gcattctatc aaagattatg ccgtctgaaa gcggcagggg
    14521 gcagattcgc gccctctgcc ggacgttcag acggcattta aacaggaaaa ccgccgctgt
    14581 tgaaacggtt ttgcctgatt ttgcctaaag gctgccgatg gcggaagcaa tacgttccgc
    14641 accgtcgcgc gcccaatccg cctgccgcgc ctccaccatc acgcgcacga cgggttcggt
    14701 tcccgaagcg cgcaacacga cacgcccttt gccttcgagt tctttttcca cttccgccaa
    14761 cacgtctttt gaggcttcct gccagttttg ccctttgccg atacggacat taatcatcgt
    14821 ttgcggatac ggctgccagt cggcgcaaac ggcggcaagg tcttggttca gcgtttgcaa
    14881 tgccgccaaa acctgcaagg cggaaataat gccgtcgccg gtgttgtgtt tgtccatgca
    14941 caaaatatgg ccgctggctt cgccgccgac gagccagccg cgttggttca gctgttccaa
    15001 cacatagcgg tcgccgactt tggcgcggca gaaatccacg ccctgctctt tcagggcgat
    15061 ttccatcgcc atattggtca tgaccgtgcc gaccacgccg ccgatgttga taccttctcg
    15121 ggcgcgggct ttagcaatga cgtaaatcag gctgtcgccg tcgtaaacct taccgttttt
    15181 atcgaccatc atcaggcggt cgccgtcgcc gtctaaggcg atgccgtagt cggcttcatg
    15241 ctgtaaaacg gcggcctgga gtgtcttggt ataagtcgca ccgcattttt cgttgatgtt
    15301 gtagccgttg ggttcgtcgc cgatgctgac gacctgtgca cccagttcgt gaaacacctt
    15361 gggtgcgaca ccgtaccccg cgccgttggc ggtatcgata accaacttca aaccacgaag
    15421 gtcggaatgg ctgggaaagg tggatttgca aaattcgata tagcggtcgt ccgcaccgct
    15481 gatacggcgt gcgcgcccga ggcgtgcaga aggctgggtt ttcatttctt cgtcgatttt
    15541 ggcttcgatt tccaactcga tttcatcgga aagcttcacg ccaccctcgg caaagaattt
    15601 gatgccgttg tcggaatagg cattatgcga cgcggaaatc atcacaccgg cggacaaacg
    15661 cagcgcacgg gtcagataag ccacgccggg cgtaggcagc ggaccggttt ggataacgtt
    15721 gacacctgcg gcggtaaaac ctgccaccaa agcggcttcc agcatataac cggaaatacg
    15781 cgtatccttg ccgattaaaa cggtcggttt ctggtcagtg tcgtgctgaa ccaaaacctg
    15841 ccccgccgca tagccgagtt tcaatacaaa atcgggcgta atcggaaact gcccgacttc
    15901 gccgcgcacg ccgtccgtgc cgaaatattt ttttgccatg tgttgctccg agaatgtgaa
    15961 ccgttgtccg agattataca gtctgtttgt gccctgctgt ctgcaccgtt gatgccgtct
    16021 gaagccgccc cgtccttttc agacggcatg aagtatgcga accgctgttt acagattgat
    16081 gcccaacgct tcccacacct tcaacgcgtc cgccgtcgcc ttcacgtcat gcacccgcac
    16141 aatttgcgcg ccgcgcgcca cagccgccac agccgccgcc acgctgccgt gtacgcgcgc
    16201 cgccgcgtct gcctcgccag tcagctcgcc tatcatacgt ttgcgcgaca cgccgatgag
    16261 cagcggaaaa cccgtttccg ccatcaattc gggtaaatgc cgcatcagtg cgatattgtg
    16321 ttgtaaggtt ttgccgaaac cgaaaccggg gtcgagtgtg atgcgttgcg gggcgatgcc
    16381 tgccgcgatg cattccgccg cccgcgcttt caaataccgc gccacttcac caacaacatc
    16441 ctgatatttc ggattaatct gcatggtttc aggcaaaccc tgcatatgca tcaggcaaat
    16501 acccgtgtcc gtctggcgcg ccagcaattc gaccgcgcct tcgtcggtca acgccgccac
    16561 atcgttaata atatcgatgc cgccgagtgc caacgctttt tccataacca ccgtgcggcg
    16621 cgtgtccaaa ctgacgggaa cgccccatcc cgcgacttct accaatacag gctcaacccg
    16681 cgcccattct tcttcggacg aaacataatc cgcacccggc cgcgtcgatt cgccgccaat
    16741 gtcgagaatg tccgcaccct ctttcaaaag ccgttcggcg tgcgccaaag cggttttggc
    16801 attttgcgaa tacgcgccgc cgtcggaaaa agaatcaggc gtgagattca cgatgcccat
    16861 aattttcggc ttgtccaaac cgatttcaaa ccgtcctgcc tgccaaccgt gtcgtgccat
    16921 ctgaactcct cccaaaataa aaaaac
//
LOCUS       DAXHMX010000027        16746 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHMX010000027 DAXHMX010000000
VERSION     DAXHMX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.027"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..371)
                     /locus_tag="ACFM4S_000665"
     CDS             complement(<1..371)
                     /locus_tag="ACFM4S_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6050394.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLAR"
     gene            complement(459..2564)
                     /gene="fusA"
                     /locus_tag="ACFM4S_000666"
     CDS             complement(459..2564)
                     /gene="fusA"
                     /locus_tag="ACFM4S_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6050395.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2583..3053)
                     /gene="rpsG"
                     /locus_tag="ACFM4S_000667"
     CDS             complement(2583..3053)
                     /gene="rpsG"
                     /locus_tag="ACFM4S_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6050396.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(3171..3542)
                     /gene="rpsL"
                     /locus_tag="ACFM4S_000668"
     CDS             complement(3171..3542)
                     /gene="rpsL"
                     /locus_tag="ACFM4S_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6050397.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3721..3870)
                     /locus_tag="ACFM4S_000669"
     CDS             complement(3721..3870)
                     /locus_tag="ACFM4S_000669"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6050398.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            complement(3867..4124)
                     /locus_tag="ACFM4S_000670"
     CDS             complement(3867..4124)
                     /locus_tag="ACFM4S_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050399.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMPINIG"
     gene            complement(4416..8591)
                     /gene="rpoC"
                     /locus_tag="ACFM4S_000671"
     CDS             complement(4416..8591)
                     /gene="rpoC"
                     /locus_tag="ACFM4S_000671"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6050400.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            complement(8748..12926)
                     /gene="rpoB"
                     /locus_tag="ACFM4S_000672"
     CDS             complement(8748..12926)
                     /gene="rpoB"
                     /locus_tag="ACFM4S_000672"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6050401.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YGNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAAEKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTKEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(13116..13487)
                     /gene="rplL"
                     /locus_tag="ACFM4S_000673"
     CDS             complement(13116..13487)
                     /gene="rplL"
                     /locus_tag="ACFM4S_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6050402.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(13545..14045)
                     /gene="rplJ"
                     /locus_tag="ACFM4S_000674"
     CDS             complement(13545..14045)
                     /gene="rplJ"
                     /locus_tag="ACFM4S_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6050403.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(14275..14970)
                     /gene="rplA"
                     /locus_tag="ACFM4S_000675"
     CDS             complement(14275..14970)
                     /gene="rplA"
                     /locus_tag="ACFM4S_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6050404.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(14970..15404)
                     /gene="rplK"
                     /locus_tag="ACFM4S_000676"
     CDS             complement(14970..15404)
                     /gene="rplK"
                     /locus_tag="ACFM4S_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6050405.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(15505..16041)
                     /gene="nusG"
                     /locus_tag="ACFM4S_000677"
     CDS             complement(15505..16041)
                     /gene="nusG"
                     /locus_tag="ACFM4S_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6050406.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(16043..16321)
                     /gene="secE"
                     /locus_tag="ACFM4S_000678"
     CDS             complement(16043..16321)
                     /gene="secE"
                     /locus_tag="ACFM4S_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6050407.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(16437..16512)
                     /locus_tag="ACFM4S_000679"
     tRNA            complement(16437..16512)
                     /locus_tag="ACFM4S_000679"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16477..16479),aa:Trp,seq:cca)
     gene            complement(16519..>16746)
                     /locus_tag="ACFM4S_000680"
     CDS             complement(16519..>16746)
                     /locus_tag="ACFM4S_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6050408.1"
                     /translation="HTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVEL
                     IAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         4202 a         4330 c         3751 g         4463 t
ORIGIN      
        1 cgggccagca ggatgtgttc gcgggtttgc ggcatagggc cgtcggctgc ggaacatacc
       61 aggattgcac cgtccatttg tgcggcgccg gtaatcatgt ttttaacgta gtcggcgtgc
      121 cccgggcagt ctacgtgtgc gtagtggcgg gtttcggttt cgtattccac gtgcgaggtg
      181 ttaatggtaa taccgcgtgc tttttcttcg ggtgcgttgt cgatttggtc gtaagctttt
      241 gcagcaccac cgaatttttt agccaaaata gtagtcaaag cggcagtcag ggtggtttta
      301 ccatggtcaa cgtgaccgat ggtgccaacg tttacgtgcg gtttgctacg ttcgaatttt
      361 tccttagcca tgagctaatt cctttacatt aaagatcgat taaagaacaa tgccgtctga
      421 aaaatcattt cagcctttcg gacggcctat ttgcctgatt agcctttacg ggcttcagtt
      481 acagcagcag ctatgtgagc aggagcttca gaatatttct tgaactccat agagtaagta
      541 gcgcggcctt gggttgcaga acgcaggtcg gtcgaataac cgaacatttc tgccagaggt
      601 acttcggcac ggactttttt accgccgata ccgtcatcat ccatacccaa tacaacaccg
      661 cgacggcggt tcaagtcgcc cattacgtcg cccatgtatt cttccggagt ttccacttca
      721 actgccataa tcggctcaag cagggcagga gatgcttgac gcataccttc tttaaacgct
      781 tgagaagcgg ccaattcaaa tgccaattgg gaagagtcga catcatggta agaaccgaat
      841 accagacgga tacgtacatc aactacagga tagccggcaa cgataccgtt aggcaatgta
      901 tcgcggatac ctttatcgac agacggaatg aattcgcgag gaatcacgcc acctttaatt
      961 tcatcgataa actcgtaacc ttcgcccccc ggttccatag gttccatttc aatcacaacg
     1021 tgaccatatt gacctttacc accggattgt ttcgcgtgtt tgtattcagc tttaacggct
     1081 ttgcggatag tttcacggta agccacttga ggtgcaccga tatttgcttc cacaccgaat
     1141 tcgcgtttca tacggtcaac aataatttcc aagtgcagct cacccatacc ggaaataatg
     1201 gtttgaccgg attcttcgtc tgtacggaca cggaaagaag ggtcttcttt agccaagcgg
     1261 ttcagggcga tacccatttt ctcttggtcg gctttggttt tcggctcaac ggcaatatgg
     1321 attaccggct cggggaattc catacgttcc aagataatcg gcgcgctttc cgcacacaag
     1381 gtttcaccgg tagtaacgtc tttcagacca atagcggctg cgatgtcgcc ggcgcgtact
     1441 tcttcgattt cagtacggtc tgcggcagtc atttgtacca aacgaccgat acgttcgcga
     1501 gtgcctttta cggagttcaa tacggtatcg ccggatttta ctacgcctga gtaaacgcgg
     1561 ataaaggtca gctgaccgac gtatttgtcg ttcaacattt tgaacgccaa tgcagagaat
     1621 ttctcttcat cgctggcttg acggctgtcg gcttcctcgg tattcgggtt gacaccttga
     1681 accggaggaa tatcggtagg agctggcagc aattctacaa ctgcgtccaa catacgttga
     1741 acacctttgt ttttaaatgc agaaccacac agcataggct gaatttcgcc tgccaaagta
     1801 cgttgacgca acgcgcctac gatttcttct tcggtcagct cgtcgccgcc taagtatttg
     1861 tccatcagtt cttcgctggc ttcggctgcg gcttcaatca tattttgacg ccattcttcg
     1921 gcagtttcga ccaattcggc aggaatatcg ccataggtaa aggttgtacc tttatcggct
     1981 tcattccaaa tgatggattt cattttcaac aaatcaacca caccgctgaa gttgtcttcc
     2041 gcaccaaccg gaatgacgat aggtacaggg tttgcgcgca aacgggtttt catttgctcg
     2101 acaacgcgga agaagttggc accctgacgg tccattttat tgacaaacgc caagcgcggc
     2161 acttggtatt tgttggcttg ccgccatacg gtttcagatt ggggttgaac accgcccacc
     2221 gcgcagtaaa ccattaccgc gccgtccaat acacgcatag aacgctctac ctctacggta
     2281 aagtcaacgt gtcccggggt gtcgatgatg ttgaagcggt gctcggggaa ttgtttcgcc
     2341 ataccggacc agtaggaagt aacggcagcg gaggtaatgg taataccgcg ctcttgctct
     2401 tgttccatgt agtcggtagt agccgcaccg tcatgcactt cgcccagctt gtgggtcaaa
     2461 ccggtataga acaaaatacg ttctgtcgtc gtggttttac ccgcatcgat atgggcggaa
     2521 ataccgatgt tacggtacag gctgatcggg gtcttacgag ccattttatt agcctttcaa
     2581 aattagaaac ggaagtgaga gaatgctttg ttggcttcag ccatacggtg tacttcttca
     2641 cgttttttca acgcaccgcc acggccttcg gacgcatcaa tcaattcgcc tgccaaacgc
     2701 aggtccatgg atttctcacc acgtttgcgg gccgcatcgc gaacccagcg cattgccaaa
     2761 gccaaacggc gtgaaggacg aacttcaaca ggaacttggt agtttgcacc acctacacgg
     2821 cggcttttca cttccacgat aggtttggcg tttgcaatgg cttcgttaaa tacttcgatt
     2881 gctactttgc cggttttttt ctcaatctgt tccaacgcac cgtaaacgat acgctcggca
     2941 acggattttt taccgtcaat catcaatacg ttcatgaatt tggtcaactc gacgctgccg
     3001 aatttaggat ctggcagtac gtcgcgcttg gggacttctc tacgtcttgg cattttaatt
     3061 tcctttaatc tattcagtcg ggtctattcc catgaatacc caattgagta ttcacttact
     3121 cggccgttgt tcagcttagg cggccgacgt gcctatttaa gtcccagtaa ttatttagga
     3181 cgcttagcac cgtatttgga acgggcttgt ttacggtctt taacgcctgc agtatccaaa
     3241 gaaccgcgta cagtgtggta acgcacacct ggcaagtctt ttacacgacc gccgcgaatc
     3301 aatacgacac tgtgctcttg caggttgtga ccttcgccgc cgatgtatga aatgacttca
     3361 aaaccgttgg tcaggcggac tttacatact ttacgcaatg cagagttagg ttttttaggg
     3421 gtagtcgtgt atacacgggt gcacacgccg cgtttttgcg ggcaggcttc cagtgcgggc
     3481 actttgttta cgtacacggg cttttgacgg cctttgcgta ccaattggtt gatagttggc
     3541 atattttctc gtcctgttga gttaaatact tgccgacacc atgtcggcaa gagtggaatt
     3601 atatttttat tgcaagtata cagtcaagca taacgacaag aagaaagatg attttcttgt
     3661 ttgccgataa atatttaccg acatccctca caatcagtcg gacgactgaa gaaaatactg
     3721 tcaatacgtt ttttctatac tgacaaaccg ttccttgccg ctgcgcgaat aaaaggcggg
     3781 catattgctg ttgatcttgt tgtaacggaa atttatttgg ggtgtaaatc ctttccacga
     3841 cagtttttta tgccataaag ccgcgctcat ccgatgttaa tcggcattcc tatccggtca
     3901 ccccgaacat cgccgcctgc cgcgctttta ggaacttcag acggcattgc atcgcgaacc
     3961 ggcggtacat cctcggcaaa cggcaggagg gcgaggcttt ccccttcaga cggcatcgca
     4021 atccgattac cgacgttaat cagttcgaca ggtacgccga tatcctcggc aaacgtcgcc
     4081 gaagtcagca ggcagcctgc aaaaaagaga tttctgggtt tcatatttat tttttaaagg
     4141 aaatgccgtc tgaagtttca gacgacattt gtttaaatgg aaaacattaa gacgctactt
     4201 tttttgaccg ccgaaaccaa catccctagc aaagttattt tggtctccgg acgtaccgac
     4261 gacacccgcc acctcgttgg tctccggccc gaaaatacct aaggaataat ccacacgcct
     4321 caaaatgacg aaaacgcttg tttttgcctt aatcagacaa agaaaaaccg caagcttaaa
     4381 gcttgcggtt ttgtattgaa taaacgcacc aaagattatt catccgttac ttgggtttca
     4441 gcagtctcct gttccacctc ttgccattgt tgatgacggc tgcggtggta agtcaaaccg
     4501 gtaccggcag gaatcaggcg gccgacgatg acgttctctt ttaaaccgcg cagttcgtct
     4561 tgtttgccca taatcgcggc ttcggtcaga acacgggtcg tttcttggaa cgatgcggca
     4621 gaaatgaaac tgtcggtgga cagggaggct ttggtaatac ccagcaatac gttttcgtaa
     4681 cgtgccggct ctttgccttc ttccaaagct ttttcattgg ccgccatcac atcgccgcgt
     4741 tcgacctgct ctccggtaat gaacccggtt tcgccggcat ccgcaatgtt cacacggcgc
     4801 aacatttgac ggatgatgac ttcgatgtgt ttatcagaaa tcttcacacc ttgcagacgg
     4861 taaacctctt gcacctcttg gacaatgtag cgtgccagtg cttcgatacc ttgcaaacgc
     4921 agaatatcat gcggatcaac agctccgtcc acgatggttt caccgcggtt taccacttga
     4981 ccgtcgtgta ccagaatttg tttctctttg gaaatcaagg tctcgtatgc tacaccgtcc
     5041 acgtcagtaa caatcagacg ttgcttgcct ttggtctctt tgccgaagga aacggtaccg
     5101 gtaatttccg ccaacatacc ggcatctttc ggcacgcgtg cttcaaacaa ttcggcaacg
     5161 cgcggcagac caccggtaat gtcgcgggtt ttggaagagg cttgcgggat acgcgccaat
     5221 acgtcgcctt taccgatttc ctgaccttcg cgtacggtaa tcaccgcacc aacggggaat
     5281 gccatcgaaa ccggagtgga cgtaccggga atacagattt ccacgccgtt ttcatccaac
     5341 aattttacag tcggacgcag cagtttggac gcgctgctgg aacggcgttt accgtcaatc
     5401 accaccaaag tggacaaacc ggttacatcg tcagtttgtt tcgcaacggt aacaccctct
     5461 tccacgttct caaatttcac catacctgca tgttcggtga tcatcggacg ggtatgcgga
     5521 tcccaggttg ccaaggtttg accggcttta atggccatac cgtcttgtac cagcaggatg
     5581 gcaccgtaag gtactttgtg gcgttcgcgt tcacggccga tatcgtcgtg aatcacgact
     5641 tcacaagaac ggccgatgac aaccaactcg cctttattgt tggcaacata acgcatttgg
     5701 ctgctgaatc gtgccgtacc gttggatttg gcttccactt ggctggctgc tgccgcacgg
     5761 gatgccgcac caccgatgtg gaacgtacgc atagtcagct gggtacccgg ttcgccgatt
     5821 gattgggcgg caatcacacc aactgcctca ccggcattga ccagcttacc acgtgccaag
     5881 tcgcgaccat aacagtgcgc gcacaaaccg tggcgggttt tacaagtaat cggcgtacgg
     5941 actttgactt catcgacacc ggattggtca atcatatcca ccagtttttc agtcaacaac
     6001 gtaccggctt caaccaaggt ctcgccgctt gacggatcga caacgtcaga cgcagtaaca
     6061 cggcccaaaa tacgatcgcg caatgcttca atcacatcac cgccttgtac cactgccttc
     6121 ataacaaagc cgtctgaagt accgcaatcg tcttcaacaa cgaccaaatc ttgagttacg
     6181 tctaccagac gacgggtcag gtaaccggag ttcgcggttt tcaatgcggt atccgccaaa
     6241 cccttacgcg caccgtgggt cgcaataaag tattgcaata cggtcagacc ttcgcggaag
     6301 tttgaggtaa tcggcgtttc aataatcgag ccgtcaggtt tcgccatcaa accgcgcata
     6361 cctgacaact gtttaatctg agccgcagaa ccacgggcac cggagtctgc catcatatag
     6421 atggagttga atgactcttg atcgacttcg ttgccggcac ggtcgataac tttttgtttg
     6481 gacaggttgt ccatcatcgc cttagcgatt ttatcgccgg cacgacccca aatatcgacc
     6541 accttgttgt agcgttcgcc gttggtaacc aaaccttgac ggtattggtc ttcgatttct
     6601 ttaacctcgg cattggcttc agccagcaag gccgcttttt cttttggaat ttccatatcg
     6661 tcaacggcaa tggaaatacc gccttttgcc gcaaatccga aaccggtgta catcaggtgg
     6721 tcagcaaaga taaccgtatc gcgcaagccg cacaggcgga acgatgcgtt aatcagttta
     6781 gaaatttctt ttttcttcaa cgctttgttg acatattcaa acggcaggcc tttcggcagg
     6841 atttcgctca acaatgcacg gccgacggtt gtttcgtaac ggttaacgac aggctcaaac
     6901 tcacctgctt cgtttttcac ccattcgcgc agacgtacgg tgatttttgt acccagctcg
     6961 acctgtttgg tatggtatgc gcgatgcact tctttcacat cggcaaacag gctgccttcg
     7021 cctttggcat tgatacggtc gcgggtcatg tagtacagac ccaatacgat atcttgggaa
     7081 ggtacaataa tcggttcgcc gttggcagga gacaatacgt tgtttgaagc cagcatcagc
     7141 gtgcgtgctt ccatttgtgc ttccaagctc aatggaacgt gtaccgccat ttggtcgccg
     7201 tcaaagtcgg cgttgaacgc ggcacacacc aatgggtgca actgaatcgc tttaccttca
     7261 atcaagatag gttcaaacgc ttgaataccc aaacggtgca gggtcggcgc acggttcagc
     7321 ataatcggat gttcgcggat gacttcttcc aagatgtccc atacttccgg tacttcttgc
     7381 tctaccaatt ttttcgctgc tttaacggtg gaagccagac cttgtttttc caatttgtgg
     7441 aaaatgaacg gtttgaacag ttccaaagcc atttttttcg gcagaccgca ttggtgcaga
     7501 cgcaggtatg ggcctacggt aatcacggaa cggccggagt agtccacacg tttgcccaac
     7561 aggttttgac ggaagcgacc gcctttacct ttaatcatgt ctgccaatga tttcagcggg
     7621 cgtttgttgg cgccggtcat ggctttaccg cgacggccgt tatccaacag cgagtcaact
     7681 gcttcttgca acatacgttt ttcgttgcgg acgatgatgt cgggcgcatg cagttccaac
     7741 agacgtttca gacggttgtt acggttaata acgcggcggt acaaatcgtt caaatcggaa
     7801 gtggcaaaac gaccaccatc caatggaacc aacggacgca aatcaggcgg caataccggc
     7861 agcacatcca taatcatcca ttccagtttc ataccggaac gatggaaggc ttccaatact
     7921 ttcaagcgtt tggcgatttt tttgattttg gtgtcggaac cggtcgattc caactcttgg
     7981 cgcaggattt cgatttcgcc cgctacattc agggtacgca gcaattcgcg gataccttcc
     8041 gcacccattt tggcatcgaa atcgtcgccg tattcgtcca gcttgttgta gtaatcgtct
     8101 tcagtcagca attggcggcg ttgcagcgga gtcataccgg gatcggttac cacaaatgct
     8161 tcaaagtaca atacgcgctc gatgtcgcgc aaagtcatgt ctaacaccat acccaagcgg
     8221 gaaggcaggg attttaagaa ccaaatatgt gcgacgggcg cagccaattc gatatgaccc
     8281 atgcgttcgc ggcgcacttt ggacagggtt acttccacgc cgcatttttc acacgttacg
     8341 cctttaaatt tcaagcgttt gtattttccg cacaagcatt cgtagtcttt gaccgggcca
     8401 aagattttgg cacagaacaa accgtcacgc tcaggtttga acgtacggta gttgatggtt
     8461 tcaggttttt tgacttcgcc ataagaccat gagcggatgg tttcgggaga ggcaataccg
     8521 attttaatgg catcaaactc ttcttccatg ccggcagttt gcaacggatt aaataagttc
     8581 aacaaattca tttttgctcc ttgaaggaag tatttttacc gcatggcgga ttttcgatat
     8641 tgggaaacgg tacttttgat ttcagacggc ctaaatgccg attgcttcat tcttattctg
     8701 aaaccacttt ttcagacgac cctgaaaggc cgtctgaaaa cttttgttta gtaacgttcc
     8761 aaatcgatat ccaagcccag tgagcgaatc tctttgacca atacgttgaa ggactcgggc
     8821 ataccggcat cgattttgtg ttcgcctttg acgatgtttt cgtacatttt ggtacggccg
     8881 ttcacgtcgt cagacttcac agtcagcatc tcttgcagcg tgtatgccgc gccgtatgct
     8941 tccaatgccc aaacctccat ctcgccgaaa cgttggccgc cgaactgggc tttaccgccc
     9001 aaaggctgct gggtaaccag actgtacgga ccggtagaac gcgcgtgcat tttttcgtca
     9061 accaagtggt gcagtttcag atagtgcatc acacctactg taaccttgcg gtcaaatgct
     9121 tcgcctgaac ggccgtcata cagcgtgatt tgggttttac tgtcgttgaa gcccagtttt
     9181 tcaacctcag gatcgtcgct cggataagcc aagttcagca tttcgcggat ttcagactct
     9241 ttcgcaccgt cgaatacagg agaggcgaaa gatgcacctt tgcgcaggtt ggaggccagt
     9301 tcgatgattt cttcatcagt cagggcatcc aaatcttctt tcttaccgct gccgttgtag
     9361 agtctgttca agaactcgcg caactcgcct gctttgcgtt gctctttcag catacggtcg
     9421 atacgctcgc cgataccttt tgctgcccaa cccaagtgaa cttccaaaat ctgaccgatg
     9481 ttcatacggg aaggtacgcc caatgggttc agtacgatgt ctaccggacg gccgtccgcc
     9541 atgtaaggca tgtcttccac tggcagaatg cgcgatacca cgcctttgtt accgtggcgg
     9601 cccgccattt tgtcgccggc ttgcagacgg cgtttgatgg cgataaaaac tttcaccatt
     9661 ttttgtacgc cgggttgcaa ttcgtcgcct tgggtcagtt ttttcttctt gatttcgtat
     9721 aactcgtccg cttcttcgcg tttttgttgc aggctcactt taatcagttc caactgcttg
     9781 gccaaatctt catcggtcag acggatatcg aaccaatcgt gcctgctcgg cagacccgcc
     9841 aaatattcgg tagtaatttc gctgccttta gccagcttca tcggaccacc attggctttt
     9901 tgacccacaa tcatacgttc gatacggtcg aatgcgtcgt tgtcgaaaat acgcaattgg
     9961 tcgttcaaat ccaaacggta gcgtttcaac tcggaatcga taatggattg ggcgcgtttg
    10021 tcgcgttgaa taccttcacg agtgaagact tgaacgtcga taaccgtacc gctcataccg
    10081 gtaggcatac gcaatgaagt atcttttacg tcagacgctt tttcgccgaa gatggcgcgc
    10141 aacagttttt cttccggcgt cagctgggtt tcgcctttag gcgttacttt gcctaccaac
    10201 acatcgccgg cttctacttc tgcaccgatg taaacgatac cggattcgtc caagcggttt
    10261 tgcatacgct cggacaagtt cggaatatcg cgggtaatgt cttccgcacc cagcttggta
    10321 tcgcgggcaa cgacattcaa ttcctcaatg tgaatcgaag tgtaacgatc atccgcagcc
    10381 actttttctg aaatcagaat cgagtcttca tagttgtaac cgttccatgg catgaaggcg
    10441 atggtcatgt tttgacccaa agccaattcg cccaagtcgg tagacgcacc gtcggccacc
    10501 aagtcgccgc gttgcaacac gtcgcctgct tttacggccg gacgttggtt gatgttggta
    10561 gattggttgg aacgggtaaa tttaaccaag ttgtaaatat cgacacccac ttcgccggca
    10621 gtcgcttcgt cgtcatggac acgaatcaca acacggttgg catcgacata ctcgaccacg
    10681 ccgcctcggc gggcaacgat tgcagtagca gagtcaacgg caacggaacg ctcgataccg
    10741 gtaccgacca tcggtttttc aggacgcaag caaggcactg cctgacgttg catattcgca
    10801 cccatcaatg cgcggttcgc gtcatcgtgt tccaagaacg gaatcaggga tgctgcaacg
    10861 gataccactt gaccagttgc cacgtccata tattggacgc ggtcgggcgt tgccataatg
    10921 gtttcgcctt tttcacgaca ggtaaccaaa tcgccaatca gattgccatc tgaatccaaa
    10981 tcggcattcg cctgtgcaat cacatagcgg ccttcttcga tggcagacaa gtaatcgatt
    11041 tccttggtta ctttgccgtc gataacgcgg cggtaaggcg tttccaagaa accgtaatca
    11101 ttggtgcgcg cataaacgga caatgagttg atcaaaccga tgttcggacc ttcaggcgtt
    11161 tcaatcggac atacgcggcc gtagtgggtc ggatgcacgt cccgtacctc gaagccggca
    11221 cgttcgcggg tcaaaccgcc cggacccaat gcggatacgc ggcgtttatg ggttacttca
    11281 gacaaggggt tggtctgatc cataaactga ctcaattggc tggagccgaa gaattcttta
    11341 atagcggcag aaacaggttt tgcattaatc aaatcgtgcg gcatcaagtt ttctgattcc
    11401 gcctgattca aacgttcttt tacagcacgt tccacacggg ccaaaccgct gcggaattgg
    11461 ttttcagtca gctcgcctac cgaacgtact cggcggttgc ccaagtgatc gatatcgtca
    11521 acttcaccat ggccgttacg caactcgacc aaagtcgcaa tcgaggcgac aatatcttcg
    11581 acgctcagga cataaccgcc tttttcggca gcaccggcaa acgtttcgtt cagcaggcgg
    11641 ccgtaccaag agttttgttg ggcttcggac agtttttgtt cgtatgtgcg cgtattaaat
    11701 ttcatacggc ctacgcggga caaatcgtag ctgtcttcac tgaagaacaa gcggttaaac
    11761 aattgctcga ccgcctcttc ggtgggcggt tcgcccggac gcatcatacg gtaaatcgca
    11821 acacgcgccg cctgccggcc ggcagtctca tccgtacgca aggtgttgga gatataagca
    11881 ccctgatcca actcattgat gtaaagggtc gtaatttctt ttacgccgtt gatatcaaat
    11941 ttggccaaca actcttctgt aatttcatca ttggcagaag ccaatacctc gccggtttcc
    12001 gaatcaatca gatcggcagc caatgcttta cccagcaggc tttccggttc tacatccaaa
    12061 cgggtcaggc ctgcattggt aatatcacgg atatttttcg cagtaatgcg cttacctttg
    12121 gcaaccaata cattgccttc tttatccaag atatcgacct tggcagtttc gcctttcaga
    12181 cggcctgcga ccaaatcggt ttgaacaccg tttgaagaca aatagaacgt ttctttgtcg
    12241 tagaaaatat ccaagatttg ctcattgccg tagcccaaag ccttcaacaa aatcgttacc
    12301 ggcattttac ggcggcggtc gatacggaaa tacagcaaat ctttcgggtc aaattcaaaa
    12361 tccaaccatg aaccgcggta gggaatgata cgggcggaga ataacaactt gccggaagag
    12421 tgcgtcttac ctttgtcatg ctcgaagaat acgccgggcg aacggtgcaa ctgggagaca
    12481 atcacacgct ctgtgccgtt aatcacaaaa gaaccgctcg gggtcatcaa cggaatttcg
    12541 cccatataca cttcgttttc acgaacttct tttaccgtcg gtttagatgc ttccttatcc
    12601 aaaatcacca aacggatacg cgcgcgcaag ggggctgcat aagtgattcc gcgcaactga
    12661 cattcgggaa tatcgaacaa aggctcgccc aatgtgtaat gcacaaactc caatcgcgca
    12721 taaccgttat ggctcacaat cgggaaaata gaattaaatg ccgcctgcag accgtcatcg
    12781 gtacgtttgt caaaagcatt ttccagctgc aaaaacttcg cataagaatc aatttgggtt
    12841 gctagcaaga aaggaacttc caaaacattt tcccgctttg caaaactctt acggatacgt
    12901 tttttctcgg taaacgaata gttcatacac actccgaaaa gtaattttca ataataggcc
    12961 gtctgaaaca acggtacgca tcaatctgat atttacattt atttgcaatg acttgataaa
    13021 aactatgcaa ataaatgtaa acaatacttg atgtttattt taagaagcaa aataaggctg
    13081 gcagaaaact gccagccttc ataagaagca tcaaattatt tgatttcgac tttagcgcct
    13141 gcttcttcca gttgtttttg gatgtcttcg gcttcagctt tagaaacacc ctctttaatg
    13201 gttttaggtg cgccgtcaac gatgtcttta gcttctttca gacccaaacc ggtaattgca
    13261 cggacaactt taatcacgcc gactttttga tcaccggcag aagccaaaac gacatcaaat
    13321 tcggtttttt cttcagcatc ggcagcaccg gcaccagcag gacctgcaac tgcaacagca
    13381 gcagcagaaa caccgaattt ttcttcaaaa gctttaacca agtcgttcaa ttccattacg
    13441 gtcaaagaac caactgcttc caaaatgtct tctttagtaa tagccatgct attttactcc
    13501 aaatattgta ttaaaaaata attgattaac agaaacaaaa tcgattaagc ggcttcttcg
    13561 ccggcttttt tctctgccaa agcagccaaa ccgcgcgcaa agcccgatac aggagcttgc
    13621 ataacgaaca acagtttgga caacagctct tcgcggctcg gaatagaagc caactcagca
    13681 acctgagcag cattcattac ttcgccattg taagaaccgg ctttaacgac aattttgtca
    13741 tcttttttcg cgaattggtg caacacttta gcagcagcaa cagcatcttc agaagcagcg
    13801 taaaccaacg gaccaaccat ttgatcggcc aattctgcaa atgaagtacc ttgcactgca
    13861 cgacgagcca aagtattttt cagaacacgc aaataaacgc cttctttacg tgcattcgca
    13921 cgaagctcag tcatactgga aacactgata ccgcgatatt cagcgactac gagggtttga
    13981 gcgttagcaa ttgccgcgct aatttcctcg accgccactt tcttggtttc aatattgaga
    14041 ctcaaggtct acctcccact gtttataaac aggataccaa aacgatatcc caccagcgcg
    14101 gtaacctaaa aagacatctt acaagcaatc ttgcaatgtg tttcaggact accgtctgcg
    14161 tagggcagtt acgattaaat cttacgatcc ctacggtctt ggacatctac ttaattttaa
    14221 gtagcccaaa ttcaagcagc ccagaaaacc aaactgcttg aaaattcctt aagattagtt
    14281 atttacgctt gatgtatcaa cgcgaatacc caaacccatg gtgctagaca cagcaacttt
    14341 tttcagatac tgacctttag cggcagcagg cttggcttta acgatagcat ccagcaacgc
    14401 atcaaagttc tctttcaaat cagcttcagc gaatgaagca cgaccaatcg ttgcatgaac
    14461 aatacctgcc ttgtcggtac ggtactgcac ttgacctgct tttgcatttt ttactgcttc
    14521 ggcaacatta ggggtaaccg tacctacttt agggtttggc atcagaccac gaggacccaa
    14581 gatggtaccc aattgaccaa caatacgcat tgcatcggga gaagcaataa caacgtcaaa
    14641 gttcagattg cctgctttga tttcagcagc caaatcttcg aaaccgacga tatctgcacc
    14701 agcttcttta gcagcttctg catttgcacc ttgagtaaat acagccacac gggttatctt
    14761 accggtgcct ttaggcagaa cgaccgaacc acggataact tggtcagatt tacgcggatc
    14821 aacgcccaag ttgaaagata cgtcaacaga ctcgtcaaat ttagcagtcg ctgctttttt
    14881 taccaaagca attgcttcat caattgcata taatttattg gcttccacag aagagcgaag
    14941 agctttcaag cgtttagata ctttagccat tatacaacac cctccacatc caagcccatt
    15001 gagcgagcag aacctgctat agtacggaca gccgcatcca agtcagcagc agtcaaatca
    15061 ggatctttag ttttagcaat ttcttccaac tgggcacggg tcaatttacc cactttgttg
    15121 gtcagaggat tagaactacc tttttgcaaa ccggcagcct ttttcaacaa gatagaagct
    15181 ggcggggttt tcatcacaaa tgtgaatgat ttatctgcaa atgcagtaat cacaaccgga
    15241 atcggtaagc caggctccat accttgggtt gcagcattaa atgccttaca aaattccata
    15301 atattcaaac cgcgctgacc caaagcagga ccaaccggag gagatggatt ggctttacct
    15361 gcaggaattt gcagtttaat atagccgata attttctttg ccacttaacg aactcctaaa
    15421 aacgggtaag acgcggaacc atttccgctc cccaagttaa tttttgattc tatcctctta
    15481 ttgctttttt ttcaagtata aaaatcagtt aatcttttca acctggctga actccagctc
    15541 aacgggtgtt tctctaccaa atatctgaac agacacgcgt aacttattcc gttcataatt
    15601 gacctcctca accaccccgt taaaatccgc aaacggccct tcatttacac gaacctgttg
    15661 accgacctca aattcaactt ttggtttcgg cttctctatg ccggtctgaa cctgctgtaa
    15721 aataatttca gcctctctct gactaatcgg cgtaggtcta ttagccctcc ctccaataaa
    15781 accggaaaca cggggggtgc tttttacaag atgccaagag tcatctgtca tttccatctc
    15841 aactagcaca taaccaggat atgactttct ttcactaata gtcttacgac cattgcggat
    15901 atcaacaact ttctctacag gcaccagaat ttgtccgaaa taatctccca tctcctcacg
    15961 ggcaatgcgc tcttccaata ttcgttggac attcttctca aaccccgaat acgcctgtac
    16021 aacataccat tttttcgaca tctcaacctt cccttctcag caatacatca aaaaataacc
    16081 acgaaattgc tgtatctgcc gcatagataa atatagaaag cacagcaaca aacactataa
    16141 caaatacagt cattctgaca gcatcttcac gcttaggcca aaccaccttt ttgaattcgg
    16201 accaagaatt tgagaaatat gcaaaaaacc cttccttacc ggaattagat gcagattctt
    16261 tatcttgaac aaccagttga tccactttaa cgttcttttt ttcaggcgta tgttctgtca
    16321 tattatttat ccatcatagc atctgtctat tctcaatcca tgtaaatggc aagagagttt
    16381 actaaataac aaatacaaaa aaaattaacc ggcacaaggc cggttaattt tttatttggc
    16441 aggccaagag ggtctcgaac ccccaaccct cggttttgga gaccgatact ctaccaattg
    16501 agctattggc ctctaaactt aagcgataac agaagaaacc acgccggcac ccacggtacg
    16561 gccgccttcg cgaatcgcaa agcgcaaacc ttcttccata gcgataggcg caatcagttc
    16621 tacggtgatg gttacgtttt cacccggcat taccatttcc acaccttctt ccaaagtaac
    16681 cgcgccggtt acgtcggtgg tacggaagta gaattgcgga cggtagttgg cgaagaacgg
    16741 ggtatg
//
LOCUS       DAXHMX010000028        16745 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHMX010000028 DAXHMX010000000
VERSION     DAXHMX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.028"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            264..908
                     /gene="rplC"
                     /locus_tag="ACFM4S_000681"
     CDS             264..908
                     /gene="rplC"
                     /locus_tag="ACFM4S_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6050409.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            908..1528
                     /gene="rplD"
                     /locus_tag="ACFM4S_000682"
     CDS             908..1528
                     /gene="rplD"
                     /locus_tag="ACFM4S_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6050410.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1525..1839
                     /gene="rplW"
                     /locus_tag="ACFM4S_000683"
     CDS             1525..1839
                     /gene="rplW"
                     /locus_tag="ACFM4S_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6050411.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1845..2678
                     /gene="rplB"
                     /locus_tag="ACFM4S_000684"
     CDS             1845..2678
                     /gene="rplB"
                     /locus_tag="ACFM4S_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6050412.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2685..2963
                     /gene="rpsS"
                     /locus_tag="ACFM4S_000685"
     CDS             2685..2963
                     /gene="rpsS"
                     /locus_tag="ACFM4S_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6050413.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            2972..3301
                     /gene="rplV"
                     /locus_tag="ACFM4S_000686"
     CDS             2972..3301
                     /gene="rplV"
                     /locus_tag="ACFM4S_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6050414.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3311..4003
                     /gene="rpsC"
                     /locus_tag="ACFM4S_000687"
     CDS             3311..4003
                     /gene="rpsC"
                     /locus_tag="ACFM4S_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6050415.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            3987..4403
                     /gene="rplP"
                     /locus_tag="ACFM4S_000688"
     CDS             3987..4403
                     /gene="rplP"
                     /locus_tag="ACFM4S_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6050416.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4403..4594
                     /gene="rpmC"
                     /locus_tag="ACFM4S_000689"
     CDS             4403..4594
                     /gene="rpmC"
                     /locus_tag="ACFM4S_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6050417.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4594..4857
                     /gene="rpsQ"
                     /locus_tag="ACFM4S_000690"
     CDS             4594..4857
                     /gene="rpsQ"
                     /locus_tag="ACFM4S_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6050418.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5080..5448
                     /gene="rplN"
                     /locus_tag="ACFM4S_000691"
     CDS             5080..5448
                     /gene="rplN"
                     /locus_tag="ACFM4S_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6050419.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5460..5783
                     /gene="rplX"
                     /locus_tag="ACFM4S_000692"
     CDS             5460..5783
                     /gene="rplX"
                     /locus_tag="ACFM4S_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6050420.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5793..6332
                     /gene="rplE"
                     /locus_tag="ACFM4S_000693"
     CDS             5793..6332
                     /gene="rplE"
                     /locus_tag="ACFM4S_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6050421.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6335..6640
                     /gene="rpsN"
                     /locus_tag="ACFM4S_000694"
     CDS             6335..6640
                     /gene="rpsN"
                     /locus_tag="ACFM4S_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6050422.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6656..7048
                     /gene="rpsH"
                     /locus_tag="ACFM4S_000695"
     CDS             6656..7048
                     /gene="rpsH"
                     /locus_tag="ACFM4S_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6050423.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7062..7595
                     /gene="rplF"
                     /locus_tag="ACFM4S_000696"
     CDS             7062..7595
                     /gene="rplF"
                     /locus_tag="ACFM4S_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6050424.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRTFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7609..7962
                     /gene="rplR"
                     /locus_tag="ACFM4S_000697"
     CDS             7609..7962
                     /gene="rplR"
                     /locus_tag="ACFM4S_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6050425.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            7981..8499
                     /gene="rpsE"
                     /locus_tag="ACFM4S_000698"
     CDS             7981..8499
                     /gene="rpsE"
                     /locus_tag="ACFM4S_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6050426.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8492..8677
                     /gene="rpmD"
                     /locus_tag="ACFM4S_000699"
     CDS             8492..8677
                     /gene="rpmD"
                     /locus_tag="ACFM4S_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6050427.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLCRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8679..9113
                     /gene="rplO"
                     /locus_tag="ACFM4S_000700"
     CDS             8679..9113
                     /gene="rplO"
                     /locus_tag="ACFM4S_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6050428.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9125..10435
                     /gene="secY"
                     /locus_tag="ACFM4S_000701"
     CDS             9125..10435
                     /gene="secY"
                     /locus_tag="ACFM4S_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6050429.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10440..10658
                     /gene="infA"
                     /locus_tag="ACFM4S_000702"
     CDS             10440..10658
                     /gene="infA"
                     /locus_tag="ACFM4S_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6050430.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10679..10792
                     /gene="rpmJ"
                     /locus_tag="ACFM4S_000703"
     CDS             10679..10792
                     /gene="rpmJ"
                     /locus_tag="ACFM4S_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6050431.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10858..11220
                     /gene="rpsM"
                     /locus_tag="ACFM4S_000704"
     CDS             10858..11220
                     /gene="rpsM"
                     /locus_tag="ACFM4S_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6050432.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11240..11635
                     /gene="rpsK"
                     /locus_tag="ACFM4S_000705"
     CDS             11240..11635
                     /gene="rpsK"
                     /locus_tag="ACFM4S_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6050433.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11655..12275
                     /gene="rpsD"
                     /locus_tag="ACFM4S_000706"
     CDS             11655..12275
                     /gene="rpsD"
                     /locus_tag="ACFM4S_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6050434.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12301..13287
                     /gene="rpoA"
                     /locus_tag="ACFM4S_000707"
     CDS             12301..13287
                     /gene="rpoA"
                     /locus_tag="ACFM4S_000707"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6050435.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13311..13679
                     /gene="rplQ"
                     /locus_tag="ACFM4S_000708"
     CDS             13311..13679
                     /gene="rplQ"
                     /locus_tag="ACFM4S_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6050436.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13826..14539
                     /gene="minC"
                     /locus_tag="ACFM4S_000709"
     CDS             13826..14539
                     /gene="minC"
                     /locus_tag="ACFM4S_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6050437.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14568..15383
                     /gene="minD"
                     /locus_tag="ACFM4S_000710"
     CDS             14568..15383
                     /gene="minD"
                     /locus_tag="ACFM4S_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6050438.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15387..15650
                     /gene="minE"
                     /locus_tag="ACFM4S_000711"
     CDS             15387..15650
                     /gene="minE"
                     /locus_tag="ACFM4S_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6050439.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15654..16574
                     /gene="oxyR"
                     /locus_tag="ACFM4S_000712"
     CDS             15654..16574
                     /gene="oxyR"
                     /locus_tag="ACFM4S_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6050440.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
BASE COUNT         4593 a         3151 c         4161 g         4840 t
ORIGIN      
        1 catcaaccat attccggttt gtcggagaaa gatgcatacg ctgtgatgac cggataccga
       61 ccctttaaaa gagtccgacc ctatgccgtc tgaaaattca aaacgcttca gacggcatat
      121 tgaagatatt tctgatattt ctgttgatat ttctttaact tgtcagatat aatgccgagc
      181 ttggtacatt tgtgccaagt ttaactttgt ctgaaagaca ggccaatcgc agcctgtccc
      241 tttactttaa aaggaaaata atcatgactt taggtctggt tggacgcaaa gttggtatga
      301 cccgcgtgtt cgacgaacag ggtgtttctg ttccggtaac cgttttggat atgtcagcca
      361 accgcgtcac acaagtaaaa tccaaagata ctgacggcta tactgccgtt caagttacct
      421 ttggtcagaa aaaagccaat cgtgtcaaca aagccgaagc cggacacttt gcaaaagcag
      481 gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa ttagctgaat
      541 tgaaagccgg tgacgaaatc accgtttcta tgtttgaagt tggtcaactg gtcgatgtaa
      601 ccggtacctc taaaggtaaa ggtttctccg gtacgatcaa acgtcataac ttcggcgccc
      661 agcgtacttc ccacggtaac tcccgttctc accgtgttcc aggctctatc ggtatggcgc
      721 aagacccggg tcgcgtgttc cccggtaaac gcatggccgg ccaatacggc aacaccaaag
      781 caactgttca aaaattggaa gttgtccgtg ttgacgcaga acgccaactg ctgttggtta
      841 agggtgctgt tccaggtgcg gtcaacagcg atgttgtagt tcgtcccagc gtgaaagtag
      901 gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca gtctgtctgt
      961 ttctgatgct ttgttcgccc gcgaatacaa tgaagcgttg gttcatcagc tggtaaatgc
     1021 ctacttggca aacgcccgct ctggtaaccg tgctcaaaag acccgtgccg aagtaaaaca
     1081 ctcaaccaaa aaaccatggc gtcaaaaagg taccggtcgc gcccgttccg gtatgacttc
     1141 ttctccgctg tggcgtaaag gcggtcgcgc gttcccgaac aaacccgacg aaaacttcac
     1201 tcaaaaagta aaccgcaaaa tgtaccgtgc cggtatggcg actattctgt cccaattggc
     1261 tcgtgacgag cgtttgtttg cgattgaggc gttgactgcc gaaactccta aaaccaaggt
     1321 ttttgctgaa caagtgaaaa atctaggtct ggagcaagtg ttgtttgtaa ccaaacagct
     1381 cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtgttggttt tggaagctca
     1441 acaagttgat ccttatagct tgctgcgtta caaaaaagta atcatcacta aagatgcagt
     1501 tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt gattttggca
     1561 cctatcgttt ctgaaaaaag caacgtattg gctgaaaaac gcaaccaaat gacgtttaag
     1621 gttttggcaa atgcaaccaa acctgaaatc aaagctgctg ttgagctgct gtttggtgtt
     1681 caagttgctt ctgtaactac cgttacaact aaaggcaaaa ctaagcgttt tggtcgtact
     1741 ttgggccgcc gcagcgatgt taaaaaagct tatgtaagct tggctgccgg tcaagagttg
     1801 gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca atcgttaaaa
     1861 tgaagccgac ctctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa ggtttgcaca
     1921 aaggtgcacc ttatgcgcct ttgcttgaaa agaaaaattc tactgccggc cgtaacaata
     1981 atggtcatat caccacccgt cacaaaggcg gcggtcataa acaccattac cgtgttgtag
     2041 actttaaacg taacaaagac ggcattcctg ccaaagtaga gcgtatcgaa tacgatccta
     2101 accgtactgc cttcatcgca ctgttgtgct atgcagacgg cgagcgtcgt tacatcatcg
     2161 ctcctcgcgg tattcaagtc ggtgccgtat tggtttccgg tgctgaagct gccatcaaag
     2221 taggcaacac cctgccgatc cgcaacattc ccgttggtac gactatccac tgtatcgaaa
     2281 tgaaacccgg taaaggtgct caaatcgcac gttctgccgg tgcttctgcg gtattgctgg
     2341 ctaaagaagg cgcgtacgct caagtccgcc tgcgctctgg cgaagtccgt aaaatcaacg
     2401 tagattgccg tgcaaccatc ggtgaagtcg gtaacgaaga gcaaagcctg aaaaaaatcg
     2461 gtaaagccgg tgccaatcgt tggcgcggta ttcgtccgac tgtacgtggt gttgtcatga
     2521 accctgtcga tcacccgcat ggtggtggtg aaggccgtac gggcgaggcc cgcgaaccgg
     2581 tcagcccatg gggtactcct gctaaaggct accgcactcg taataacaaa cgcacggata
     2641 acatgattgt tcgtcgccgt tactcaaata aaggttaatt tagtatggct cgttcattga
     2701 aaaaaggccc atatgtagac ctgcatttgc tgaaaaaagt agatgctgct cgcgcaagca
     2761 acgacaaacg cccgattaaa acctggtctc gtcgttctac cattctgcct gattttatcg
     2821 gtctgaccat tgctgtgcac aacggccgca cccatgtgcc tgtgtttatc agcgacaata
     2881 tggttggtca taaattaggc gaattctcat tgacccgtac ctttaaaggc cacttggccg
     2941 ataaaaaggc taaaaagaaa taaggtgaat catgagagta aatgcacaac ataaaaatgc
     3001 ccgtatctct gctcaaaagg ctcgtttggt agctgatttg attcgtggta aagacgttgc
     3061 ccaagctttg aatattttgg ctttcagtcc taaaaaaggt gccgagctga ttaaaaaagt
     3121 attggagtca gctattgcta atgccgagca caataacggt gcggacattg atgaactgaa
     3181 agtggtaact atctttgttg acaaaggccc aagcttgaaa cgttttcaag ctcgcgccaa
     3241 aggtcgcggt aaccgcatcg aaaaacaaac ttgtcatatc aatgtgacag tgggtaacta
     3301 aggaaaagct atgggacaaa agattaaccc tacaggcttt cgcctggcgg taactaaaga
     3361 ctgggcttca aaatggtttg ctaaaagcac cgacttttct actgttttga agcaggatat
     3421 cgatgttcgc aattatttgc gtcaaaaatt ggccaatgct tcggttggtc gagtggttat
     3481 tgaacgccct gcaaaatctg cacgcattac cattcactcc gctcgtccgg gtgtggttat
     3541 cggtaaaaaa ggtgaggata tcgaggtttt gaaacgtgac ttgcaagtct tgatgggtgt
     3601 acctgttcat gtaaatattg aagagattcg ccgtcctgag ttggatgctc aaattattgc
     3661 tgacggtatt gcccagcagt tggaaaagcg cgttcaattc cgtcgtgcta tgaaacgagc
     3721 aatgcaaaat gcaatgcgtt ctggtgctaa aggcattaag attatgactt caggccgtct
     3781 gaatggtgcg gatattgccc gtagtgaatg gtatcgtgaa ggtcgcgtgc cactgcatac
     3841 tttacgtgca aatgtagatt atgcaaccag cgaagcgcac accacatatg gtgtattggg
     3901 tctgaaagtt tgggtttata cggaaggcaa tattaaatct tccaaacctg aacatgagag
     3961 taaacaaaga aaggcaggta gacgtaatgc tgcagccaac tagactgaaa taccgtaagc
     4021 aacaaaaggg tcgcaatact ggcatcgcta ctcgcggtaa taaggtaagt ttcggtgagt
     4081 tcggcttgaa agccgtaggt cgtggtcgtt tgactgcccg tcaaatcgaa gctgctcgtc
     4141 gtgcaatgac ccgtcatatc aaacgtggtg gtcgtatttg gattcgtgta ttccctgata
     4201 aaccgattac tgaaaagcct attcaagttc gtatgggtgg cggtaaaggt aacgtggaat
     4261 attacattgc cgaaattaaa ccaggtaaag tgttgtatga aatggatggc gttccagagg
     4321 aactggctcg tgaagcattc gagttggctg ctgccaaatt gcctattcct acaacctttg
     4381 tagtaagaca ggtgggtcaa taatgaaagc aaatgaattg aaagacaaat ccgttgagca
     4441 gttgaatgca gatttgttgg acttgttgaa agctcagttt ggcttacgta tgcaaaacgc
     4501 taccggtcaa ttaggcaaac caagtgaatt gaaacgtgta cgtcgcgata ttgctcgtat
     4561 taaaaccgtt ttaactgaaa aaggtgctaa gtaatgagcg aaactaaaaa tgttcgtact
     4621 ttgcaaggca aagtagtaag cgacaaaatg gataaaaccg taacagtatt ggttgagcgt
     4681 aaagtaaaac atccgctgta tggtaagatt attcggttat ctactaaaat ccatgcccat
     4741 gatgaaaata atcaatatgg aattggtgat gtggttgtta tatcggaatc ccgtccattg
     4801 tcaaaaacta aatcttgggt tgtcagtgag ctggttgaga aagcacgttc tatttaagaa
     4861 ttaaagcaac gtgcttggaa tgggaaacga agtattgcag caaatttaat ttgcgtgtaa
     4921 acttcgtttc ctgtctttca gtttcttctg gaagtttctt cccttttcgg ggtccaagac
     4981 tggtttactt gaaccgcaag gtttcattta ataagcagcg gctttgctgt aagttatctg
     5041 aaagtggtaa attaagttgg ttaatttaaa ggtaataaca tgattcaaat gcagaccatc
     5101 ttagatgtgg ctgataactc tggtgcgcgt cgcgtaatgt gtatcaaggt attgggcgga
     5161 tctaagcgtc gctacgcttc tgttggcgat attattaaag tggcagttaa agatgcggct
     5221 ccgcgtggcc gtgtcaaaaa aggcgatgta tataatgcgg tagttgttcg tactgctaag
     5281 ggtgtacgtc gtcctgatgg tgcgttaatt aaattcgata acaatgccgc cgtgttactg
     5341 aataataaac ttgaaccttt gggtactcgt atctttggtc cggtaacccg tgaattgcgt
     5401 actgagcgat ttatgaaaat cgtttcattg gcacctgaag tattataagg aatggcacga
     5461 tgaataaaat cattaaaggc gatagggttg tagtaattgc tggtaaggat aaaggtaagc
     5521 agggtcaagt agttcgagtg ttgggtgata aagttgttgt tgagggcgtt aatgttgtaa
     5581 aacgccatca aaaacctaat ccaatgcgtg gcattgaggg cggtattatt actaaagaaa
     5641 tgcctttgga tatttctaat atcgcaatcc tgaatccgga aactaataaa gcggaccgtg
     5701 ttggtattaa gctgattgaa aatgaaggca aagttaaacg cgttcgtttc ttcaaatcaa
     5761 atggctctat cattggggca taaggagata acatggctcg gttgagagag ttttataaag
     5821 agacagttgt tcctgaattg gttaaacaat ttggttacaa atcagtaatg gaagtcccgc
     5881 gtattgaaaa aattaccttg aatatgggtg tgggtgaggc tgttgctgat aaaaaagtta
     5941 tggaacatgc tgtttccgat ttagagaaaa ttgccggtca aaaaccggtt gttactgttg
     6001 cccgtaaatc tatcgcaggt tttaaaatcc gtgataacta tccggttggt tgcaaagtaa
     6061 cattgcgtcg tgatcaaatg tttgaattct tggatcgttt gattactatt gcattacctc
     6121 gcgtacgtga cttccgtggt gtgagcggta aatcatttga tggccgtggc aattacaata
     6181 tgggtgttcg tgagcaaatt atttttccgg aaattgaata cgataaaatt gatgctttgc
     6241 gtggtttgaa tattactatt actactacag caaaaaccga tgaggaagcg aaagctttat
     6301 tgtcattgtt taaatttccg ttcaaaggat aatcatggct aagaaagcac ttattaatcg
     6361 tgatctgaaa cgtcaagctt tggctaaaaa atatgcggct aaacgcgcgg caattaaagc
     6421 ggtaatcaat gattcgaatg caactgagga agagcgtttt gaggctcgtt tgaggtttca
     6481 atccattcct cgtaatgcgg cacctgtgcg tcaacgtcgt cgttgtgctt tgacaggtcg
     6541 ccctcgtggt actttccgta aatttggttt gggtcgtatt aaaatccgtg aaatcgccat
     6601 gcgtggcgaa attccgggtg ttgttaaagc cagctggtaa taggagtaat taagaatgag
     6661 tatgcatgat cctatttccg atatgttgac tcgtatccgc aatgcgcaac gtgctaataa
     6721 agcagcggtt gcaatgcctt cttcaaaatt aaagtgtgct attgcaaagg tattgaaaga
     6781 agaaggatat attgaggact tcgcagtttc atctgacgta aagtctatat tggaaattca
     6841 attaaaatac tatgcaggtc gtcctgtaat tgaacaaatc aagcgtgtat ctcgccctgg
     6901 tttgcgtatt tataaagcgt ctagtgagat tccaagtgtt atgaatggct tgggtattgc
     6961 tattgttagt acttctaaag gtgtaatgac tgatcgtaaa gcacgttctc aaggtgttgg
     7021 tggtgagttg ttatgcattg tagcctagtg gaggaaaaga aatgtcacgt gtcgcaaaaa
     7081 acccagtgac tgttcccgct ggtgtagaag taaaatttgg aacagaggca ttagttatta
     7141 agggtaagaa cggtgaattg tcttttcctt tgcattctga tgtagccatt gaatttaatg
     7201 atggcaaatt gacttttgtt gcgaataaca gcagtaaaca agcaaatgca atgtctggta
     7261 ctgctcgcgc attagtcagc aatatggtta aaggtgtttc agaaggtttt gagaaaagat
     7321 tgcaattgat aggtgtgggt tatcgtgctc aagcacaagg taaaatcttg aatctgtctt
     7381 tgggtttttc tcatccgatc gtatatgaaa tgcctgaagg tgtctccgtt caaactccta
     7441 gccaaacaga gattgtttta accggctcgg ataaacaagt tgttggtcaa gttgctgctg
     7501 agattcgtac gttccgtgct cctgagcctt ataaaggtaa aggtgttcgc tatgtaggcg
     7561 aagtagtggt aatgaaagaa gccaagaaaa aataattgag gttcactaat ggataaacat
     7621 acaacccgac tccgtcgtgc acgcaaaacc cgtgctcgta ttgcggactt gaaaatggta
     7681 agattatgtg tgttccgaag caataatcat atttatgctc aagtaattag tgctgaaggt
     7741 gataaagtat tggctcaagc ctctacattg gaagctgagg tgcgcggtag tctgaaatct
     7801 ggaagcaatg ttgaagcagc tgcaatagtt ggtaaacgta ttgctgagaa ggctaaagca
     7861 gcaggtgtag aaaaggttgc ttttgatcgt tcaggtttcc aatatcacgg tcgtgtgaag
     7921 gctttggctg aagctgctcg tgaaaatggt ttaagcttct aaatatttgg agactttcag
     7981 atggcaaaac atgaaattga agaacgcggt gacggtctga ttgaaaagat ggtcgcagtt
     8041 aaccgtgtaa ccaaagtagt taaaggtggt cgcattatgg ctttctctgc gctaactgtt
     8101 gttggtgatg gagatggtcg tattggtatg ggtaaaggta agtcaaaaga agtgcctgtt
     8161 gcagtccaaa aggcgatgga tcaagcacga cgctctatga ttaaggtacc attaaaaaat
     8221 ggtacgattc atcatgaggt tattggtcga catggtgcta ctaaagtatt tatgcagcct
     8281 gctaaagagg gtagtggcgt aaaagccggt ggacctatgc gtttggtttt tgatgctatg
     8341 ggcattcata atatctccgc caaagtgcac ggatctacta acccatataa tatcgtacgt
     8401 gcaacattag atggtttgtc taagttgcat actcctgctg atatcgcagc caaacgtggc
     8461 ttgacagtgg aagacatttt gggagttaac catggctgaa caaaaaaaga ttagggttac
     8521 attggttaaa agcctgattg gtacaattga atctcatcgt gcatgtgcac gcggtttagg
     8581 tttgtgtcgt cgcgagcata cggtagaggt tttagatacc cctgaaaacc gtggtatgat
     8641 taataaaatc agctacttgt tgaaagtgga gtcttgatat gtttttgaat acaattcaac
     8701 ctgctgttgg tgctacgcat gctggtcgtc gtgttggacg cggtattggt agtggtcttg
     8761 gcaaaacggg tggtcgtggt cataaaggtc aaaagagccg gtctggtggg tttcataagg
     8821 tgggtttcga gggtggtcaa atgcccttgc aacgacgcct ccctaaaaga ggttttaaat
     8881 ctttaacagc atcagctaat gcacagcttc gtttaagtga actggaatca attgctgtta
     8941 atgagattga tattttggtc ttaaagcaag cgggtctgat tgcatctaca gtctctaatg
     9001 ttaaagttat tgcttctggt gaaatttcta aggcagttgc tttgaagggt attaaagtta
     9061 ccaaaggtgc gagagctgct atcgaggctg ttggtggtaa gattgaaatg taaggtttaa
     9121 tattgtggct aatcaacaaa cgtcatcagg ttcatccaaa tttggagatc ttaagaaacg
     9181 tcttttgttt ctatttggag cattgattgt ttttcgaatt ggtgcccata tacccgtacc
     9241 tggagttgat gctgttgctt tagctaaatt atacgaaagc gctggaaacg gcatcctggg
     9301 aatattgaat atgttttccg gtgggtcgtt agagcgcttt agtatatttg caataggaat
     9361 tatgccatat atttcagctt ctattattgt acagctcgct tctgaaattt tgccatcatt
     9421 gaaggcttta aaaaaagaag gggaggctgg tagaaaggta attacgaaat atactaggta
     9481 tggtactgtt ttgttagcaa ttcttcaaag tctaggtgtt gcatctttcg tatttcagca
     9541 aggaattgtt gtaacaagtt catttgagtt tcatgtttcc acggtagttt ctttggtaac
     9601 gggaaccatg tttcttatgt ggcttgggga gcaaattact gaaaggggta tcgggaacgg
     9661 tatttcttta atcattacgg caggtattgc ttcaggtatt ccttcgggta ttgcaaagct
     9721 ggttacactg acgaaccaag gttctatgag catgcttacg gcgttgttta ttgtatttgg
     9781 tgccttatta ttaatttatt tggttgtata ctttgaaagt gcacagcgga agattcctat
     9841 tcattatgca aaacgccagt ttaatggtag ggcgggtagt caaaatacgc atatgccttt
     9901 caagttgaat atggctggtg ttattccccc aatttttgct tccagtatta ttctatttcc
     9961 atctactctt ttaggttggt ttggttcggc tgatacaaat agtgttttgc acaaaatagc
    10021 tggattgtta caacacggtc aattgctgta tatggcttta tttgcagcga cagttatttt
    10081 cttttgttat ttttatacgg ctttggtttt tagccctaaa gaaatggcag agaatttaaa
    10141 aaagagtggt gcttttgttc ctgggattag acctggtgag cagacctcta ggtatttaga
    10201 aaaagttgta ttacgtttga cattgtttgg agctctttat attacaacta tttgtttaat
    10261 tccagagttc ttaactacgg ttttaaatgt acctttttat ttgggtggca cgtctttgtt
    10321 gattctagtt gttgtaacga tggattttag tacacaaata aattcgtata ggcttactca
    10381 acagtatgat aagttaatga ctcgttcaga aatgaaatca ttttctcgga aatagaatta
    10441 tggcgaaaga agatactatc caaatgcaag gtgaaattct tgaaacttta cctaatgcaa
    10501 catttaaagt aaaacttgag aatgaccata ttgtattggg tcatatttct gggaagatgc
    10561 ggatgcatta cattcgtatt tctccgggag ataaggtcac agtagagctg acaccttatg
    10621 atctaactag ggctcgaatc gttttcagag caagataaac caataaaagg aaaataaaat
    10681 gcgtgtacaa ccatctgtta agaaaatttg ccgaaattgc aagattattc gtcgaaatcg
    10741 tgtagttcgt gtaatttgta ctgatctccg tcacaaacag cgtcaaggtt aatggaatat
    10801 ttcttttaat gtgattctgt gatatagtga cacactttgc cctaaaaagg aaaaaatatg
    10861 gctcgtattg caggggtaaa tatccctaat aacgcacaca tcgtaattgg tcttcaggct
    10921 atttacggta ttggtgctac tcgtgctaaa ttgatttgtg aggctgcaaa tattgcgcct
    10981 gatactaaag caaaagattt ggacgagact caattagatg ctttgcgtga ccaagttgcc
    11041 aagtatgaag tagaaggtga tttgcgtcgt gaggtaacta tgagtatcaa gcgattgatg
    11101 gacatgggct gctatcgtgg cttccgtcat cgtcgcggct taccatgccg cggtcaacgc
    11161 actcgtacaa atgcgcgtac ccgcaaaggt ccgcgtaaag cgattgctgg taagaaataa
    11221 attttaagga attttattaa tggctaaagc aaacacagct tcacgtgtac gtaaaaaagt
    11281 acgtaaaacc gtgagtgagg gtattgtgca cgttcatgca tctttcaaca ataccatcat
    11341 tacaatcact gaccgtcaag gcaatgcgtt gtcttgggct acctctggcg gcgctggttt
    11401 taaaggttct cgtaaaagta caccatttgc agcacaagtt gcagcagaag cagctggtaa
    11461 agttgcccaa gagtatggcg ttaaaaattt agaggttcgt attaaaggtc caggtccagg
    11521 tcgtgaatcc tctgtacgtg ctttgaatgc tcttggtttc aagattacca gcattactga
    11581 cgttaccccg ttgcctcata acggttgccg tccgcctaaa aaacgtcgta tttaatattg
    11641 gagtgatttg aaacatggca cgttatattg gccctaaatg taagttggca cgtcgcgaag
    11701 gtacggattt gtttttgaag agtgcgcgcc gctctttgga ttccaaatgt aaaattgatt
    11761 ccgctcccgg tcagcatggt gcaaaaaaac cgcgtttgtc agactatggt ttgcagttgc
    11821 gtgaaaaaca aaaaatccgc cgtatttatg gcgtattaga acgtcagttc cgtcgttatt
    11881 tcgcagaagc tgatcgtcgt aaaggttcta ccggcgagtt gctgttgcag ttgctggaat
    11941 ctcgtttgga taatgtcgtt tatcgtatgg gtttcggttc tacccgagct gaagcaagac
    12001 agcttgtttc tcataaggcg atagttgtga atggacaagt tgtcaatatt ccttctttcc
    12061 aagtgaaagc tggtgatgtt gtctcagttc gtgaaaaagc caaaaaacag gtacgtattc
    12121 aagaagcatt gggtttggca actcaaatcg gcttgccggg ttgggtttct gtagatgcgg
    12181 ataaacttga gggtgtgttc aaaaacatgc cggatcgctc ggaattgacc ggtgatatta
    12241 atgaacagct ggtggtagag ttctactcta aataatgcta gctcagtgag ggacagttaa
    12301 atgcagaata gcacaaccga atttttgaaa cctcgtcaaa ttgatgtaaa tactttttct
    12361 gcaactcgtg caaaagtatc tatgcagcca tttgaacgtg gtttcggtca taccttaggt
    12421 aatgctttgc gccgtatctt actgtcatcc atgaatggtt ttgctcctac tgaagtagct
    12481 attgccggtg tattacacga atattctact gttgatggta ttcaggaaga tgttgttgac
    12541 attttgctga atattaaagg tattgtgttt aaactccatg gtcgtagcca agttcaactt
    12601 gtgttgaaga aatcaggttc aggtgtcgta tctgccggtg atattgagtt gccgcatgat
    12661 gtagaaattc tgaatcctgg tcatgtcatt tgtcatttgg ctgataacgg tcaaattgag
    12721 atggaaatta aagtagagca aggtcgtggt tatcaatctg tttcaggtcg tcaggtagtt
    12781 cgtgatgaga accgtcagat tggtgcaatc cagttggatg cgagcttttc gcccatcagc
    12841 cgtgttagct ttgaggttga acctgcacgt gtagagcagc ggacggatct tgataagttg
    12901 gttttggata tcgaaaccga cggttctatt gatcctgagg aagctgtacg cagtgcggca
    12961 cgtattttga ttgatcagat gtctattttt gctgatttgc agggtactcc tgtggaggag
    13021 gttgaagaaa aagcacctcc tatcgaccct gttcttttgc gtccggtgga tgatctggaa
    13081 ttgacagtac gttcagctaa ttgtttgaaa gctgaggata tttattatat tggcgatttg
    13141 attcaacgca ctgaaaccga gcttcttaaa acgccgaatt tgggacgtaa atctttgaat
    13201 gagattaagg aagtattggc atctaaaggt ttgacactgg gttctaagtt ggaagcatgg
    13261 ccacctgtag gcttggaaaa gccttaatga agaattaaag gataattgat atgcgtcatc
    13321 gtaatggcaa tcgcaaatta aaccgtacca gcagtcatcg tgctgcaatg ctgcgtaata
    13381 tggcgaattc attattgact cacgaaacta ttgtaacaac tctgcctaag gccaaggaat
    13441 tgcgccgtgt agtagagccg ttgattacat tgggtaaaaa gccgtcattg gcaaaccgcc
    13501 gtttggcatt tgaccgtact cgcgaccgtg atgttgtagt aaaactgttt ggcgatttgg
    13561 gtcctcgttt tactgctcgt aacggtggtt atgttcgggt gttgaaatac ggattccgta
    13621 aaggtgataa tgcacctctg gcactggttg aattggttga caaaccggct gctgagtaat
    13681 tttagtcata taacgccatc tgccgaaaag caggtggcgt tatttttgca atatctgata
    13741 ggtaataggg tattggctat catgtttaaa atattaattg aatagctaag gtttgcgcgg
    13801 taaacttaca tcattaaaaa attctatgat ggtttatata atgaatgctt tcgatataaa
    13861 gtcgacaaag atggacgtat tgtctatatc tttgcatacg tcagacttgt ttgatttgga
    13921 agatgtgctg gtcaaattgg gcaagaagtt tcaagagtct ggtgttgttc catttgtgct
    13981 ggatgttcaa gagtttgatt atcccgagtc tttggatctt gctgcattgg tttcgttgtt
    14041 ttcaaggcat ggtatgcaaa ttttgggtct gaagcattct aatgaacgtt gggctgctgc
    14101 ggctatgaag tatcatttgc tgttttgtct gtctcattcg gaaaatgtta aagaactggg
    14161 tcaggttgag gtgcagaaca cggaggatgg tcagaaagca aggaaaacag tattgattac
    14221 atcccctgtc cgtaccggtc agcaggttta tgccgaagat ggcgatttga ttgttacggg
    14281 ggcggtcagc cagggggcgg aattgattgc agatggcaat atgcatattt atgcgccgat
    14341 gagagggcgt gctttggccg gtgccaaagg cgatacttct gcccgcatat ttatccactc
    14401 catgcaggca gaactggttt ctgtggcggg tatttaccgt aattttgaac aggatttgcc
    14461 ggaccatctg cacaagcagc cggtacagat attgttgcag gataaccgat tggttatcag
    14521 tgcaattggc tcagaataat tgtttgatat tttaaaaagg aaatattgtg gcaaaaatta
    14581 ttgtagtaac ttcaggtaag ggcggtgtcg gtaaaacgac taccagtgcc agtattgcga
    14641 caggtttggc attacgcgga tataaaactg cggtaattga ttttgatgtg ggtttgcgta
    14701 acctcgacct cattatgggt tgcgagcgtc gtgtcgttta tgacctgatc aatgtcattc
    14761 agggggaggc gacgctcaac caggctttga ttaaagataa aaattgtgaa aacctgttta
    14821 ttttgccggc ttcccagact cgggataaag acgctttgac acgcgaggga gtagaaaaag
    14881 tgatgcagga gctgtccggc aagaaaatgg gctttgagta tattatttgc gactctcctg
    14941 ccggtattga gcagggtgca ttgatggcgt tgtattttgc tgatgaagcc attgtaacga
    15001 ccaatcctga ggtttccagt gtgcgtgact ccgacaggat tttgggaatt ttgcaaagca
    15061 aatcccataa ggcagagcaa ggcggttcgg ttaaagaaca tctgttgatt acgcgttatt
    15121 ctcccgaacg tgtggcaaaa ggcgaaatgc tgtctgtaca ggatatttgc gatattctgc
    15181 atatcccttt gctgggtgtg attcctgaat cccaaaacgt cttgcaggca tccaattccg
    15241 gagaaccggt catccatcag gacagcgtgg cggcttccga ggcatataag gacgttattg
    15301 cccgtctttt gggcgagaac cgtgaaatgc gcttcttgga agctgagaaa aaaagcttct
    15361 tcaaacgtct gtttggagga taaggtatgt cattaatcga atttttattc ggcagaaagc
    15421 agaaaacggc aaccgttgcc cgcgaccgcc ttcaaatcat cattgcccaa gagcgcgccc
    15481 aagaaggtca ggctccggat tacctgccga ctttacgtaa agagttgatg gaagtcctgt
    15541 ccaaatatgt gaacgtttca ttagacaata tccgtatttc ccaggaaaaa caggatggta
    15601 tggatgtgct tgagttgaac attactttgc cggaacagaa aaaggtatag gacatgacct
    15661 taaccgaatt gcggtacatc gtcgcagtcg cccaagaacg tcatttcggc agggcggcgc
    15721 ggcgttgttt tgtcagtcag cccactttgt ctattgccat taagaaattg gaagaagagc
    15781 ttgccgtctc tttgtttgac cggagcagca acgatattat tacgaccgag gcgggggaac
    15841 gtatcgttgc acaggcgcgt aaggtattgg aagaggcgga gcttatcagg catttggcaa
    15901 atgaagaaca aaacgagctg gagggtgcgt tcaaactcgg gctgattttt acggttgcgc
    15961 cgtacctgct gccgaaactg attgtctcgt tgcgccgtac tgcaccgaaa atgcctttga
    16021 tgttggaaga gaattacacg catactttga ccgagtcgct caaacgcggg gacgttgatg
    16081 cgattatcgt tgccgaaccg tttcaagagc cgggcattgt taccgaaccc ttgtatgacg
    16141 aaccgttttt cgtgattgtc ccgaaagggc attcatttga ggaactggat gccgtttcgc
    16201 cccggatgct gggtgaggag caggttttgc tgctgacgga aggcaactgt atgcgggatc
    16261 aggtactctc aagctgttcc gaattggcgg cgaaacaacg catacagggg ttgaccaata
    16321 cattgcaggg cagctcgatc aatacaatcc gccatatggt cgccagcggt ttggcaatca
    16381 gcgtgttacc ggcaaccgcg ctgaccgaaa acgatcatat gctgttcagc attattccgt
    16441 ttgaaggtac gccgccaagc cggcgggtcg tattggcgta ccgccgcaat tttgtccgtc
    16501 cgaaggcgtt gtcggcgatg aaggcggcga ttatgcagtc gcagcttcac ggggtaagtt
    16561 ttatccgcga ctaggcgcag gcattgtttt caaaacgcca tttccctgag ccgacaacac
    16621 ggtatgccaa gatattgccg tcatcatcga ttttgagtat agcatcgcca cggaaactgc
    16681 cgtcctgaag atattcgact tttgcatcac tgtgaatgtt ttcatcagtg ccgatgcaat
    16741 gccat
//
LOCUS       DAXHMX010000029        16540 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHMX010000029 DAXHMX010000000
VERSION     DAXHMX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16540
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.029"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..325
                     /locus_tag="ACFM4S_000713"
     CDS             <1..325
                     /locus_tag="ACFM4S_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6050441.1"
                     /translation="EEGIAQTVVKGKTTRAEVEARFGKRNPFGCYAYHEVSLPIYNFL
                     PTNFIYMKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVIRKEAGKSL
                     SQPEK"
     gene            389..1672
                     /gene="hemL"
                     /locus_tag="ACFM4S_000714"
     CDS             389..1672
                     /gene="hemL"
                     /locus_tag="ACFM4S_000714"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6050442.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1888..2160)
                     /locus_tag="ACFM4S_000715"
     CDS             complement(1888..2160)
                     /locus_tag="ACFM4S_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HFC6050443.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2171..2734)
                     /gene="orn"
                     /locus_tag="ACFM4S_000716"
     CDS             complement(2171..2734)
                     /gene="orn"
                     /locus_tag="ACFM4S_000716"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6050444.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2752..3639)
                     /gene="prmA"
                     /locus_tag="ACFM4S_000717"
     CDS             complement(2752..3639)
                     /gene="prmA"
                     /locus_tag="ACFM4S_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6050445.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGETLEDQDWVR
                     LTQAQFDPIQISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAAQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            complement(3757..5118)
                     /gene="accC"
                     /locus_tag="ACFM4S_000718"
     CDS             complement(3757..5118)
                     /gene="accC"
                     /locus_tag="ACFM4S_000718"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6050446.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5232..5687)
                     /gene="accB"
                     /locus_tag="ACFM4S_000719"
     CDS             complement(5232..5687)
                     /gene="accB"
                     /locus_tag="ACFM4S_000719"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6050447.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5669..5926)
                     /locus_tag="ACFM4S_000720"
     CDS             complement(5669..5926)
                     /locus_tag="ACFM4S_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6050448.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6043..7083
                     /gene="queA"
                     /locus_tag="ACFM4S_000721"
     CDS             6043..7083
                     /gene="queA"
                     /locus_tag="ACFM4S_000721"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6050449.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            complement(7453..8031)
                     /locus_tag="ACFM4S_000722"
     CDS             complement(7453..8031)
                     /locus_tag="ACFM4S_000722"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6050450.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMT
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            8270..9169
                     /gene="crgA"
                     /locus_tag="ACFM4S_000723"
     CDS             8270..9169
                     /gene="crgA"
                     /locus_tag="ACFM4S_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6050451.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSAEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(9396..12611)
                     /gene="carB"
                     /locus_tag="ACFM4S_000724"
     CDS             complement(9396..12611)
                     /gene="carB"
                     /locus_tag="ACFM4S_000724"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6050452.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAMNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLVKEEKSVSDGILSDLDFAALRRLKRKGFSDKRIAQLLGVKEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(12640..13281)
                     /locus_tag="ACFM4S_000725"
     CDS             complement(12640..13281)
                     /locus_tag="ACFM4S_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFC6050453.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            13443..13841
                     /locus_tag="ACFM4S_000726"
     CDS             13443..13841
                     /locus_tag="ACFM4S_000726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050454.1"
                     /translation="MRYVCTRSPFSLQPQPTACVPCGTLSADEFKGRLKKRSQRLFHL
                     PCDKGCLKHSRCRCFGHSFSDDLQRLNLLQPTPSPVGEGWGEGILRISATFPNTLTAK
                     TQALWLVALSLTLSHGREDWAVVGAKVSVN"
     gene            complement(13949..14338)
                     /locus_tag="ACFM4S_000727"
     CDS             complement(13949..14338)
                     /locus_tag="ACFM4S_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6050455.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(14912..15037)
                     /locus_tag="ACFM4S_000728"
                     /pseudo
     CDS             complement(14912..15037)
                     /locus_tag="ACFM4S_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(15083..16216)
                     /gene="carA"
                     /locus_tag="ACFM4S_000729"
     CDS             complement(15083..16216)
                     /gene="carA"
                     /locus_tag="ACFM4S_000729"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6050456.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         3571 a         4206 c         4591 g         4172 t
ORIGIN      
        1 ggaagaggga atcgcacaaa cggttgttaa aggcaaaacg actcgggcgg aggtagaggc
       61 acgtttcggg aagcgcaacc ctttcggttg ttatgcctat catgaagtca gcctgccgat
      121 ttataatttt ttgccgacca atttcattta tatgaaatca gaacgccggc attgggagtg
      181 gtgtgtggat tacgactggg aaggagttgt ccgggactac cgctttacac ataaagtgga
      241 aaaagacgag cgttccgtca tccgggatgc ggttggcgtc atccgcaaag aagcaggcaa
      301 atccttgtcg caacctgaaa aatgataaaa tgggactttc tgcttctaag cccgaaacct
      361 gccgttcaga cggcatttga ggataaatat gaaccgtaac gaaattttat tcgaccgcgc
      421 caaagccatc atccccggcg gcgtgaattc ccccgtccgc gcattcggca gcgtcggcgg
      481 cgtgccgcgc ttcatcaaaa aagccgaagg cgcgtatgtt tgggacgaaa acggcacgcg
      541 ttataccgac tatgtcggct cttgggggcc tgcgattgtc ggacacgcgc accccgaagt
      601 cgtcgaagcc gtgcgcgaag ccgcgttggg cggtttgtcg ttcggcgcgc ccaccgaggg
      661 cgaaatcgcc attgccgaac aaatcgccaa aatcatgccg tccgtcgaac ggctgcgcct
      721 cgtcagctcc ggtaccgaag cgacgatgac cgccatccgt ttggcacgcg gttttaccgg
      781 tcgcgacaaa atcatcaagt ttgaaggctg ctaccacggg cattccgaca gcctgctggt
      841 gaaagccggc agcggcctgc tgacgttcgg taatccctct tccgccggtg tgcctgccga
      901 ctttaccaaa cacactttag tccttgagta caacaacatc gcccaattgg aagaagcctt
      961 tgctcaaagc ggcaatgaca ttgcctgtgt gattttggag ccttttgtcg gcaatatgaa
     1021 ccttgtccgc ccgaccgaag ccttcgtcaa agccctgcgc gaattgaccg aaaaacacgg
     1081 cgcggtgttg atttacgacg aagtgatgac cggtttccgc gtcgcgctcg gcggcgcaca
     1141 atcgctgcac ggcatcatgc ccgacctgac cacgatgggc aaagtcatcg gcggcggtat
     1201 gccgcttgcc gcgttcggcg ggcgcaaaga cattatggaa tgtatttccc cactgggcgg
     1261 cgtgtatcag gcaggtacat tatcaggcaa cccgattgcc gtcgccgccg gtttgaaaac
     1321 gctggaaatc atccgccgcg aagggtttta tgaaaacctg acggcgcgta ccgaacagtt
     1381 ggttcaaggt tttcagacgg cagcggatgc ggcgggcatc gagtttgccg ccgacagcgt
     1441 gggcggtatg ttcggtctgt atttcgccgc acacgcgccg cgaaactatg ccgatatggc
     1501 gcgttccaat attgacgctt tcaaacgctt cttccacggt atgctcgacc gcggcattgc
     1561 cttcggcccg tccgcttatg aagcaggttt cgtttctgcc gcgcatacgc ccgagctgat
     1621 tgacgaaacg gttgcggttg cggttgaagt gttcaaggcg atggctgcat gatgttttga
     1681 cggacagagt ttctctgttc gatttgtttg gcagattgaa gtaagaatgc acaccgccgt
     1741 cattcccgcg caggcgggaa tccagacctt tcagtttctg taatgattga aaataacggg
     1801 ccgaaatcag ccgcgcccgg caggaagccg ccgccacctg aaacaatgcc gtctgaagcc
     1861 ttcagacggc attttatcgg aagcgcatca gaaaaaacgg atagcgacat atgcggtaaa
     1921 agcaagggca attacataga taaacccctt tataaattca aaagtctcat ctatatcgct
     1981 atatttttct ttcagttcgt cagattttcg ataacgtttc attttcaaga ccacttcatg
     2041 accaacaagc ccgaccttcc ggattcccgc ctgcgcggga atgacgggcg tgtatatttt
     2101 tgatttcaat ctgctgtaaa aatgccgtct gaaatgcagg atttgcgctt cagacggcat
     2161 tttttgtttc ttattgcgct tcggctctcg gggcggaaat cagaaagtgt tcgcggtagt
     2221 ggcgcatttc ttcgatgctc tccaaaatgt cgtccaatgc cttgtgcgaa ccgcgtttga
     2281 cgacgctttt ggcaacgggc ggattccagc gtttggcaag ctctttcagt gtggaaacgt
     2341 caaggttgcg gtagtggaag tagttttcca gtttcggcat atatttgacc ataaaacgcc
     2401 ggtcttggtg gatggaattg ccgcacatcg gcgtggcgcg tccgggtacc cattccgaca
     2461 taaagtccag cagtttctgt tcgacttcgg cttcggtatg cgacgattcg cgcacgcgct
     2521 gcgtcagccc cgtcctgccg tgtgtggcgg tgttccactc gtccatattg tcgagcagct
     2581 cgtcgctttg gtggacggcg taaacttcgg attgcgccaa tacattcaaa tccgagtcgg
     2641 taataatcat cgcgacttcg ataatgcggt cggtttcggg attcagcccc gtcatttcca
     2701 tatcgagcca gcagaggttg tttttgtcct gcacggtgtt tccttttcgt ttcagcgttt
     2761 tacgccgctc aagcgcgccc agccttcttc ggtttccgcc gggtcgaggt cgaaccattg
     2821 gctgtaaatg ccgccgagtt cttcggcctg ttcgtccaac aaaccggaca acacgatgcg
     2881 tccgccctgt ttggtgcggg ctgcgagcat ttcgccgagc atacgcaaag ggttggcgag
     2941 gatgttggcg acaactacgt cgaattgccc ttgaggcaga ccgtcgggca ggaagaattg
     3001 tgcatcgacg ttgttttgcg cggcgttgtc cctgccggcg cggacggcct gttcgtcaat
     3061 atccacgccg acggcggaac ctgcaccgag tttgagggcg gcgatggtca ggatgcccga
     3121 accgcagccg tagtcgagga cgctttcgcc gtttttgagt tgcgtatcca gccatttgag
     3181 gcagaggcgc gtggtcgggt ggctgccggt gccgaaggcg aggccgggat cgaggcggag
     3241 gttgacggca gtgccttcgg gggcttcgtg ccaagagggg gtaatccaca ggcgttcgga
     3301 aatttggatg gggtcgaatt gcgcttgggt caggcgtacc cagtcttggt cttcaagggt
     3361 ttcgccggtg tatgccaagt cttttaatcc gcattcttgt gcggcggttt ggatgatggc
     3421 ggcggcttcg tcgtgttcgc cgaacagggc gatgactttg ctctgctgcc agatttgttc
     3481 ggcgggcata ccgggttcgc cgaaaatcgc ctgttcgttt tgcgtgccgg cgtaggcatc
     3541 ttcgatggcg gcggagagtg cgccgtgttc catcagcgcg tcggcgaggc gttcggcgac
     3601 ggcatcgttg acgttgacgg tgatttgttg gtagggcata acgggctttc ttaacagacc
     3661 ttcggaatag ggtcgtactg tataagggga tgggtgtgta acggaaatgc cgtctgaaaa
     3721 cggacaaact gtttcagacg gcatatcggc ggcggtttac ttgtcctgtt tggctttgcg
     3781 atcttccagc cagtgttcca aatagtggat gctgacaccg cctttttgga aacccgcatc
     3841 ggcgaacagg tcgcggtgaa gcggcgtatt ggttttgatg ccggttaccg ccagctcggc
     3901 gagtgcgacg cgcattttcg ccattgcctg ttcacgcgtt ttgccgtgta cgcagatttt
     3961 gccgatcagg ctgtcgtagt acggtgggat gcggtagcct tggtaaatgt ggctgtccac
     4021 gcggataccg aagccgccgg gcaggtggca gctttcaatc gggcccgggc tggggatgaa
     4081 gttgtatggg tcttcggcgt tgatacggca ctcaaacgcg tggccttcga cttgaatatc
     4141 cttttgtttg tattgcaaag gcaggccggc ggcgatgcgg agttgctcct gcacgatgtc
     4201 cacgccggtg atgagctcgg taaccggatg ctcgacctga acgcgcgtgt tcatctcgat
     4261 aaagaaaaat tcgccgtctt cgtataaaaa ctcaaacgta cccgcgcccc ggtagccgat
     4321 gcgtttgcag gcatcggcac aggcgttgcc gattttggcg cgttcttttt cagtgatgaa
     4381 cggagccggt gcttcctcga tgactttttg gtggcggcgt tgcagcgaac agtcgcgctc
     4441 ggcaaggtag atggcgttgc cgtgttcgtc ggcaatcact tggatttcga cgtggcgcgg
     4501 acgttgcaaa tagcgttcca tgtaaaccat cgggttgccg aatgccgcgc ccgcttcggc
     4561 cttggtcatt tcgacagatt ggaggaggtc ttcttttttc tcgaccacgc gcataccgcg
     4621 accgccgccg ccaccggagg ctttgataat gacgggataa ccgactttat cggcgatttt
     4681 gaggatttct tcgccgtcgt cgggcaatgc gccgtcagaa ccggggacgc agggtacgcc
     4741 tgccgctatc atcgcgtgtt tggcggagac tttgtcgccc atcaggcgga tggtgtcggg
     4801 tttcgggccg ataaaggtaa agccggactg ctcgacctgt tcggcgaaat cggcgttttc
     4861 ggcaaggaaa ccgtagcccg gatggacagc gtccgcgcag cttacttcgg cggcggcgat
     4921 aatggcgggg acgttaaggt agctttgcgc ggaagcggca gggccgatgc acacggattc
     4981 gtcggcgagt ttgacgtgca ggctgtcttt gtcggcctcg gaatgcacgg cgacggtggc
     5041 aatgcccatt tcgcggcagg cacggagtac gcgtaatgcg atttcgcctc ggttggcgat
     5101 taaaactttt ttcagcatga tgactgttcc tgtggttccg gcaacccgtc cgtaaaaaaa
     5161 aaggtgcggg aacgccgaag ggggaaagca tttcagacgg catcggaagt ttatgccgtc
     5221 tgaaaacagg attatccgat gatgaagagc ggttcgccga attcgacggg cgtaccgttt
     5281 tcgaccagaa tttctttgac cgtgccggat ttttcggctt cgatttcgtt catcagcttc
     5341 atcgcttcga tgatgcacag cgtgtcgccg gctttaacct gttggccgac ttcgacaaaa
     5401 ggcgcggcat tcgggccggg tgcgcggtag aacgtgccga ccataggcga tttttgcgcg
     5461 tcggacaaat cgcgggcggc aggtgcggcg gcgggcgcgg atgccgcaac gggtgcggcg
     5521 gcaggcgtta cggccggcgc ggcggcaggt acgggcgcgg cgtaaacggg tgcggcggcg
     5581 atggttcggg tgatgcggac tttttcctcg ccttcggtta cttcgatttc ggcgataccc
     5641 gattcttcaa ccaaatcaat cagttttttt aatttgcgca aatccatttc tgttccttta
     5701 aaggctgccg gtgttccggc gggttgttgc gttggttttt cagaaaacag ccggcttgcg
     5761 gcaacggcgc aaaccgggcg gaatggtgtt cggatacggt tttcctgtgg tggaaaacaa
     5821 ccgtgagaat gtgtttattt tcccgaagtt gtcggtaaat gtccagtaaa atatcaaaaa
     5881 acggcggttt ttgggggaaa tgtgcaggaa gtttgatttt gcgcacaaga tgttccaggt
     5941 taagagtgtt tattctaatc tgttggtttt tcggacaaag atgccgtctg aaaaagggct
     6001 aaagtgcgta taatggcggc ttgcccgaac gagagtgtaa aaatggatat ttcagatttt
     6061 gattttaccc tgcccgaaaa gctgattgcc cagcatccgc ccgaagtgcg cggcagcagc
     6121 cgccttttgg ttgcgttgcc cgatatgccg ctgcaagacc gggtgttcgg cgatttgccg
     6181 gattatgtcg aggcgggcga cgttttggtg ttcaacaaca ccaaagtcat gaaggcgcgg
     6241 ctgtttgggc agaaagacag cggcggcagg atcgaagccc tgattgagcg cgtgttggac
     6301 aaccataccg cactggcgca catccgttcg tccaagtccc ccaagcccgg tatggggctg
     6361 gtgtttgaag gcggcatccg tgccgtgatg gtcgggcgtg agggcgaact gttctgcctg
     6421 cgttttgaag gcggtcaaac cgtttacgaa cttttggaac agaacggaca cctgcccctg
     6481 ccgccttata tcgaacgcgc cgccgacgcg gacgacgaca gccgttatca aaccgtttat
     6541 gccaaatatc agggtgcggt cgccgcgccg acggcgggtc tgcattttac ggaagaactt
     6601 ttgcgccgcc tgaaagacaa aggcgcagta accgcagaag taaccctgca cgtcggtgcg
     6661 ggaacgttcc agcccgtgcg tgtggaaaaa atcgaagaac acaaaatgca cagcgaatgg
     6721 tttgaagtgc cgtctgaaac cgccgccgcc gttgaggcgg caaaagcacg ggggaacaaa
     6781 gtctgggcgg tcggcacgac ttccatgcgc gccctcgaat ctgccgcgcg tgcgacgggt
     6841 cgtctgaaag cggggcaggg cgacaccgat attttcatca cgccgggcta ccgttttaat
     6901 gttgtcgaca ggctggttac caattttcat ttgccgaaat cgacgctgct gatgcttgtc
     6961 agcgcgtttt cgggtatggg tcatatccgc gccgcgtatc gttatgcggt cgaacgcgaa
     7021 taccgtttct tcagctacgg cgatgcgatg gttttggggc ggaacgaagg ggtggtacgt
     7081 tagaacagcc ccaagtctat tgggggaaga caagtgccgt ctgaacggac gttcggacgg
     7141 cattcttttg ttgatttgaa tgaaaggaac tgtatgacat agattcaggc gcgagaggcg
     7201 agaaactcag tcattgttga tgacgcgcaa tacgttaaaa ttcattccgt tttggtcgta
     7261 taaacatatt acatacgcca tttttttgta ttcaaggctg cctgaaaaca gtgggcaaga
     7321 atatctaacc caacgaaaag ctgaaaaagc aacaaaaaat aatctgcacc ttagtcggtt
     7381 ggttagtcca accttagggg tgcagaccat tcaatctttc aggctgcctt tgtttgttta
     7441 taagataaat ttttagccga acactttttc caagtgtgcc tgataatctg ccaagtattt
     7501 ttccacttgt ggatttttaa ccacatcgtt acataagaat gtcggcaggc gggtcatacc
     7561 caaaaactcg ttggctttgt ggaagtgcat atacaaaaca tcaacgcctt tgccttcaaa
     7621 gaaatcgcct tcgcgggtaa acgcctcaat cggcgcattc caagtcagtg aaagcatatg
     7681 ttttttgcct tgcaacaagc cgcctgtgcc gtagccctca gtcggattga cgcggtgtct
     7741 gccgtcgctt tggtagagtt tgccgtgtcc agcggttaat acttcgtcta tgtatttttt
     7801 cactgtccaa ggctcgtgca tccaccagcc cggcatctgc caaatcacag catccatcca
     7861 aacgaatttt tcgatttctg cctcaacatc atagccggca tcaatcacgg tttcttgaac
     7921 attgtgtccg agcgcggtca aaacttcttt cgcttttttg tgaagcgtgc ggtttaaccc
     7981 gccgtgagaa tgtccgaacg ctttgccgcc gtctaataat aaaatattca tttgttacct
     8041 cgtttgtgaa ttgatgaatg tattgtgccg ttttacttta taatttaaaa gtgcaaaaat
     8101 aagaaaacac ttttgcgtca aatgaaataa tcagatgttc caacacacgg gatggcacat
     8161 aaagcaccgc cctgtgtgcc gtcctgattc ggaaggggtt acacccctcc caaataaagt
     8221 ctgatcctgc cgccctaaag ggcggggttc caaccgaaaa ggaaacacga tgaaaaccaa
     8281 ttcagaagaa ctgaccgtat ttgttcaagt ggtggaaagc ggcagcttca gccgtgcggc
     8341 ggagcagttg gcgatggcaa attctgccgt aagccgcatc gtcaaacggc tggaggaaaa
     8401 gttgggtgtg aacctgctca accgcaccac gcggcaactc agtctgacgg aagaaggcgc
     8461 acaatatttc cgccgcgcgc agagaatcct gcaagaaatg gcagcggcgg aaaccgaaat
     8521 gctggcagtg cacgaaatac cgcaaggcgt gttgcgcgtg gattccgcga tgccgatggt
     8581 gctgcatctg ctggcgccgc tggcagcaaa attcaacgaa cgctatccgc atatccgact
     8641 ttcgctcgtt tcttccgaag gctatatcaa tctgattgaa cgcaaagtcg atattgcctt
     8701 acgggccgga gaattggacg attccgggct gcgtgcacgc catctgtttg acagccgctt
     8761 ccgcgtaatc gccagtcctg aatacctggc aaaacacggc acgccgcaat ctgcagaaga
     8821 gcttgccggt caccaatgtt taggcttcac cgaacccggt tctctaaata catgggcggt
     8881 tttagatgcg cagggaaatc cctataagat ttcaccgcac tttaccgcca gcagcggtga
     8941 aatcttacgc tcgttgtgcc tttcaggttg cggtattgct tgcttatcag attttttggt
     9001 tgacaacgac atcgctgaag gaaagttaat tcccctgctc gccgaacaaa cctccaataa
     9061 aacgcacccc tttaatgctg tttattacag cgataaagcc gtcaacctcc gcttgcgcgt
     9121 atttttggat tttttagtgg aggaactggg aaacaatctc tgtggataat tctataaaaa
     9181 cctgccataa aattgttttt actcacttaa taactttgag aaaaaataaa taagatgatg
     9241 tatagtcggc ggcaatggca gtctatatgg ggagcgtgat agggctgttc cttatgtacg
     9301 gcttgtgtgt ggcaacatcg gctttccaaa tgacaaaatg ccgtctgaca ccgtaaaaaa
     9361 aggtttcaga cggcattttc aacccgattc aggcatcagc tgcggttttt caaacgcccg
     9421 tgcagttctt gaacgctgta cacgcccaag tggtcgcggc ttttcgcgcc ttcgctcatc
     9481 gcttcgccgc ccgcagtagt ggtgtattgc ggcacacgtt gctgcaatgc gctttggcgg
     9541 atgatgtggc tgtcggacac ggattgcgga tcgctggaaa cggtattcac gaccagtgcg
     9601 atttcgccgt ttttcagcgc gtcgccgatg tgcgggcggc cttcggggac tttattgatg
     9661 gtttgtacaa tcagcccgtg ttcggtcagg tattgcgccg tgccgcgcgt ggcgcagatg
     9721 ccgtagccta aggcttggaa gtttttggcg gttttaatga cgcgttcttt gtcttcttcg
     9781 cgcacggaga ggaagatttt gccggtcggg ttgaggcgtt cgcccgcgcc gagttgggct
     9841 ttgtagtagg cttcgccaaa gcttgcgccc acgcccatga cttcgccggt ggagcgcatt
     9901 tccggtccca aaatcgtatc cacgcccggg aatttgatga atgggaacac ggcttcttta
     9961 acggcataga aatcggggac aacttctttt tccacgcctt gttctttcag ggaaatgcct
    10021 gccatgcagc gcgcgccgac tttggcgagc ggcacgccgg tggctttgga gacgaagggc
    10081 acggtgcggc tggcgcgcgg gttcacttcc aatacgaaca ccacgccgtc ttgtacggca
    10141 aactgcacgt tcatcagtcc gaccacgccc agcgcgtacg ccatcgcttt agtttggcgg
    10201 cggatttcgt cttggatttc ttcgcttaag gagtagggcg gcagcgagca gccggagtcg
    10261 ccggagtgga tgcccgcctg ttcgacgtgc tgcatgatgc cgccgataac cacgtctttg
    10321 ccgtctgaaa cgcagtccac atccacttca atcgcgttgt tgaggaagaa atcgagcaac
    10381 acggggctgt cttcggaaac ctgcacggct tcgcgcatgt atttttgcaa ggcttcggca
    10441 gagtggacaa tctgcatcgc gcgtccgccc aaaacataag acgggcgcac gaccagcgga
    10501 tagccgattt cttcggcttt gacgagtgct tcttcttcgt tgtgggcgat gcggttgggc
    10561 ggttggcgca gacctaagtc gttcaacact ttttggaagc gttcgcggtc ttcggcggcg
    10621 tcgatgctgt cggcggacgt gccgatgatg tttacgccgt tttcaaccag cgcgttggcg
    10681 agtttgagcg gggtttgacc gccgtaatgc acaatcacgc cccacgggtt ttcggtgcgg
    10741 acgatttcca acacgtcttc caatgtcagc ggctcgaaat agaggcggtc gctggtgtcg
    10801 aagtcggtgg acacggtttc tgggttgcag ttgaccataa tcgtttcaaa accggattcg
    10861 cgcagggcga gcgcggcgtg aacgcagcag tagtcaaact cgatgccctg accgatgcgg
    10921 ttcgggccgc cgccgagaat catcactttt ttacggtcgg aaggacggga ttcgcattct
    10981 tcttcgtaag tggagtaaag ataggcggtt tcggtggcga actcggcggc gcaggtgtca
    11041 acgcgtttgt aaaccggatg cagcttcagc gcgtagcggt gttcgcgcac ttctttttct
    11101 tttacgccca aaagctgcgc gatgcgtttg tcggagaagc ctttgcgttt cagacggcgt
    11161 agggcggcga aatccaaatc actcaaaatg ccgtctgaaa ccgatttttc ttctttcacc
    11221 aagtcttcga tttgcgccaa gaaccaaggg tcgatggcgc agatttcgtg gatttcttcc
    11281 ggcgtgaagc ccgcgcggaa cgcgtctgcc acaaacagca tgcgctcggg gccggggttt
    11341 gccagttcgc ggcggatttc cgctttgtcg gagcttctcg gattgaagcc gcacaagcct
    11401 gtttccaagc cgcgcagggc tttttggaaa ctttcctgaa tcgtgcgtcc catcgccatc
    11461 acttcgccca ccgatttcat ctgcgtggtc aggcggtcgt ctgcggcagg gaatttttca
    11521 aacgcaaaac gcgggatttt ggttaccaca tagtcgatgg acggctcgaa tgacgcgggg
    11581 gttttgccgc cggtgatgtc gttgcgcaac tcgtccagcg taaagccgac cgccagtttc
    11641 gccgccacct tcgcaatcgg gaagcccgtc gctttggaag ccagcgcgga tgaacggctc
    11701 acgcgcgggt tcatctcaat cacaatcatc tcgccgtttt cagggttcat cgcaaactgc
    11761 acgtttgagc cgcccgtgtc cacgccgatt tcgcgcaata ccgccaacga agcgttgcgc
    11821 atgatttggt attccttgtc cgtcagcgtt tgcgccggcg cgaccgtaat cgagtcgccc
    11881 gtatgcacgc ccatcgggtc gaagttttca atcgagcaga tgatgatgca gttgtcgttc
    11941 ttgtcgcgca ctacttccat ctcgtactct ttccagccga gaacggactg ctcaatcagc
    12001 agctcgtgcg tgggcgaggc atcgaaaccg cgttcgcaaa tcgccaaaaa ctcgtcttta
    12061 ttgtaggcaa tgccgccgcc cgaaccgccc atcgtgaaag aaggacgaat cagggtaggg
    12121 aagccgacct gttcctgcgc cgccaaagct tcgttcattg tgtggcagac aaaagatttc
    12181 gggcaagaca aaccgatttt ttccatcgcc tctttaaagc ggccgcggtc ttctgctttg
    12241 tcgatggcgt cttccgtcgc gccgattaat tcaacattgt atttcgccag cacgccgttg
    12301 cgcgccaaat ccagcgcaca gttcagcgca gtttgaccgc ccatggtagg cagaatcgca
    12361 tcgggccgct ctttggcaat aattttttcc accgtctgcc acataatcgg ctcgatgtag
    12421 gtaacatccg ccatttcggg gtcggtcata atcgtggcgg ggttggaatt caccaaaatg
    12481 actttatagc cttcttcacg caaggctttg caggcctgtg cgcccgaata gtcaaattcg
    12541 caggcctgac cgataacgat agggccggcg ccgatgataa ggatggattt taggtcggta
    12601 cgtttgggca tgatgggtct ttcttttgaa gaatttggat tattgacgat tggtaacttc
    12661 tctaattatt tctgcatctg ttttttcatc gcttggatta tctgaaaaga aaaaactgta
    12721 attactatcg gctttaaatt tttcgtatag ctctgggcgc aaaatttttg ctttttcact
    12781 attttccacg ctcctgcgat taggcttgcc agccttatct ttcccatttc tacggacatc
    12841 atagcaataa atagtgttag gagaattagg ttctaaacga gtagctacaa tctttttata
    12901 aattatgtct aaatcttttt tagtttgtgt tggaatgaaa tctgcaaaaa gattactaaa
    12961 actctgaaaa atttctctga ttttcgcagc accagcctga acattaattt taaaaaaatt
    13021 aataaaatca gtagcacttt taaaatgaac ttcataaaaa tcagcatacg caaatattga
    13081 attttcacta ttcaaattaa aaaactctaa ttttgcttgt gcataatcat ctggtctagg
    13141 ttcatcaggt ttatacctag tcaaaataac ggcgtattgc ctttgattat aattaaaagg
    13201 aaatgcttca attattctat ttttgtttct catatcatta agcaaatact ttaagttatt
    13261 gaaattaata ggtattgaca tatttaaact cctgcgaata tgttgcaata aacgtagcaa
    13321 tcgtaggttg ggttgaaaac ccaacacaaa ccgcttaatt gtctaaaatg ttgggtttgc
    13381 taacctaact tgtatatcga aacccccgta tcgtagcaag ccgtagcccg tatgtagggt
    13441 atgtgcggta cgtatgcacg cgttctccat tttccctgca accccaaccc accgcgtgtg
    13501 tgccttgcgg cacactctct gccgatgagt tcaaaggtcg tctgaaaaaa cgttcgcaac
    13561 gtttgttcca cctgccttgc gataaaggct gcctgaaaca ttcccgctgt cgatgtttcg
    13621 ggcattcttt ttcagacgac ctgcaacgcc tgaatctgct gcaacctacc ccctcccccg
    13681 tgggggaggg ctggggagag ggcattctcc gaatttcggc aacctttcct aacactctaa
    13741 ccgccaagac acaagccttg tggcttgttg ccctctctct aactctctcc cacgggagag
    13801 aggactgggc ggttgttggg gctaaggttt ctgtaaacta atcaggttat gcaggctgct
    13861 ttttgttttt cagacggcct ttataaatgc tcaattcagt cggctttatc ctattatttt
    13921 caataatcca atcaaaatca gccatctgct atcgtgcagc ttgcttttcc aattcttgca
    13981 acacgagcag gatttctgtc agcacatcgt ttgtctgctg taaaatctcg tgattccaaa
    14041 accgcagtac ggtaaagccc aagctgttga gatatgccgt ccgcgcgtgg tcgtatgcgg
    14101 cttgttctgt gtgctgccct ccgtctgctt cgacaatcaa cttgggcgtt acgcacataa
    14161 aatcaacaat atagtttccc atcggctgct ggcggcggaa tttatagccg ttcagacgac
    14221 ctgcccgcag gtgctgccac aattttgctt ccgccccgct catttcttgg cgcatggctt
    14281 tggcgcgttg gcgcaagatg gggttgtcgg tggtcaagag tttctcgtgc gggttcattt
    14341 tgttgtcgtg ctgtttgctg ccctctctct aactctctcc cacggggaga gaggacgggg
    14401 cggttgttgg ggttaaaggg gctgtaagga aaacaagtcg ttcaggccac tttttacttt
    14461 tcagacgacc tgcaatcaac ggttgtaaat ccaagttgct tttcaaaccg cctgtaaccg
    14521 caaaacttgc tgcaacctac cccctccccc gtgggggagg gctggggaga gggcattctt
    14581 cgaatttcgg caaccttccc caacacccta accgcccaaa cacaagcctt gctgtttgtt
    14641 gccctctctc taacgctttc ccacagggag acaggacggg gcggctgtag tgattaaggg
    14701 ttttgcaagg aaaacaagga aaacaagttg tacaggctgt ttttttattt ttcaagcgac
    14761 ctgaaatcaa cgcttgtaaa tccaagtcgc ttttcaaact gcctgcaacc gcaaaatctg
    14821 ctgcaaccta ccccctcccc cgtgggggag ggctggggag agggcatttt ccaagttgcg
    14881 gcaatctttc ccaaatccct tagtcttcta aaatacaagc cttacggttg ttgccaaatg
    14941 aaacttcttc catacccaaa acttgcgtgt aaaacgcaat ggttcggtca atgtcggcaa
    15001 cggtcagtac cagatggtcg agtgcgctaa tcttcatttc ccgctttctt tttcaggcgg
    15061 cggcagtgcc gtctgaaagc cattatgccc gttttgccgc tttcatattg ccaatgaatt
    15121 tgtcaaacaa atagccgaca tcttgcgggc cggggctggc ttcggggtgg ccttggaagc
    15181 agaacacagg tttgtcggtc agctcgatgc cttgcaaagt gttgtcaaac aaggatttgt
    15241 gggtaatgcg tgcgttagcg ggcagggtgt cggcatcaac ggcaaaaccg tggttttggc
    15301 tggtgatgac gactttgccg ctgtccaaat cttgcacagg gtggttcgca ccgtggtggc
    15361 tgaagcgcat tttcagggtt ttcgcgccga tagcgaggct gatgagctgg tgtcccaagc
    15421 agatgccgaa aatcggtttg ccgctttcca tcagtttttg cacggcttcg atggcgtagg
    15481 tgcaaggctc ggggtcgccg gggccgttgg acaggaacac gccgtcggga ttgagtgcca
    15541 acacgtcttg tgcgctcgtt tgggcgggga cgacggtcag gcggcagccg cgcgaggcga
    15601 gcatacgcag gatgttggtt ttcacgccga aatcgtaggc gacgacgtgg taaggctgtt
    15661 cgtcaggggt aacgaaaccc ttgcccaatg cccattcacc ttccgtccat tcgtaagttt
    15721 ccgtgcagga aacttctttt gccaagtctt tgccgaccat gctgccgaac gcggcgatga
    15781 gttcttgcgc tttttcgacc gtagcgtccg cgccggtcag aatcgcaccg ccttgcgcgc
    15841 ctttttcacg caacagcgtg gtcaggcggc gggtgtcgat gtcggcgatg gcaacggttt
    15901 ggttgcgcac cagatagtcg tgcaggcttt cggaggcgcg gaagttgctg tgcaagagcg
    15961 gcaggtcgcg gataatcaaa ccggcggcgt aaacgctgcg gctttcttca tcttcggcgt
    16021 tggtaccggt attgccgatg tgtgggtagg tgagggtaac gatttgtttg cagtaggacg
    16081 ggtcggtcag gatttcctga tagccagtca tcgaagtatt gaacacgact tcgccggaag
    16141 tcgaaccttc gtaaccgatt gatgtgccgt ggaatacgct gccgtcagcg aggacgagga
    16201 gggcgggggt gctcatgatg ggaatcctgt ttttaataaa attcttgaca atgccgtctg
    16261 aagggacttc ggcagggggc atccggtcgg aaaaatccgg tcaaaaaaaa acacgccgcg
    16321 caagatagac gcgtaacgtg cttttcgggc aagtgcctat accctgaaag cagcatattt
    16381 taagacgaaa catacccgat ttcaagcgca aacgcggcag gcgggctgat atgttcgcgc
    16441 gaaaacggaa atccgtggcg taaaaccgcc cgctccgcgc ccactatgcc cgttacgcat
    16501 ttccccacgt ccgtccggcg aaactatggg aatacccgaa
//
LOCUS       DAXHMX010000030        16450 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHMX010000030 DAXHMX010000000
VERSION     DAXHMX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.030"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            209..1273
                     /locus_tag="ACFM4S_000730"
     CDS             209..1273
                     /locus_tag="ACFM4S_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6050457.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="ACFM4S_000731"
     CDS             1414..1737
                     /locus_tag="ACFM4S_000731"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6050458.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="ACFM4S_000732"
     CDS             1809..2132
                     /locus_tag="ACFM4S_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6050459.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEVVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQLLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="ACFM4S_000733"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="ACFM4S_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6050460.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3069
                     /gene="tatB"
                     /locus_tag="ACFM4S_000734"
     CDS             2386..3069
                     /gene="tatB"
                     /locus_tag="ACFM4S_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6050461.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLSDGISDVMPSERSDTSAETLGNDGQIGGTAEPSE
                     TDKDRAWREYLTAPSSPAVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSRA
                     KPKLRVRKS"
     gene            3084..3854
                     /gene="tatC"
                     /locus_tag="ACFM4S_000735"
     CDS             3084..3854
                     /gene="tatC"
                     /locus_tag="ACFM4S_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6050462.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKHARPYVIVGAFVIAAVITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPAKT"
     gene            3907..4512
                     /locus_tag="ACFM4S_000736"
     CDS             3907..4512
                     /locus_tag="ACFM4S_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6050463.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVVKLPADIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPHSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIGGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4528..4881
                     /locus_tag="ACFM4S_000737"
     CDS             4528..4881
                     /locus_tag="ACFM4S_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6050464.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4871..6682
                     /locus_tag="ACFM4S_000738"
     CDS             4871..6682
                     /locus_tag="ACFM4S_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6050465.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDIV
                     SWIFILLAVSAWACKSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPLL
                     QNLIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVLG
                     LVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVLLTALFQFSMNTILETFT
                     GIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQHN
                     IHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLICT
                     LAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFAG
                     LLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALPE
                     YPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPRY
                     ADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6872..7549
                     /gene="misR"
                     /locus_tag="ACFM4S_000739"
     CDS             6872..7549
                     /gene="misR"
                     /locus_tag="ACFM4S_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6050466.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7562..8968
                     /gene="misS"
                     /locus_tag="ACFM4S_000740"
     CDS             7562..8968
                     /gene="misS"
                     /locus_tag="ACFM4S_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6050467.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9086..11512
                     /locus_tag="ACFM4S_000741"
     CDS             9086..11512
                     /locus_tag="ACFM4S_000741"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6050468.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHSLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKNLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11557..11802
                     /gene="rpsP"
                     /locus_tag="ACFM4S_000742"
     CDS             11557..11802
                     /gene="rpsP"
                     /locus_tag="ACFM4S_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6050469.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11818..12327
                     /gene="rimM"
                     /locus_tag="ACFM4S_000743"
     CDS             11818..12327
                     /gene="rimM"
                     /locus_tag="ACFM4S_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6050470.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLHGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12327..13076
                     /gene="trmD"
                     /locus_tag="ACFM4S_000744"
     CDS             12327..13076
                     /gene="trmD"
                     /locus_tag="ACFM4S_000744"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6050471.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLSYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRLLETIRQEQREIQS"
     gene            13092..13457
                     /gene="rplS"
                     /locus_tag="ACFM4S_000745"
     CDS             13092..13457
                     /gene="rplS"
                     /locus_tag="ACFM4S_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6050472.1"
                     /translation="MNLIQQLEQEEIVRLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13779..14504
                     /locus_tag="ACFM4S_000746"
     CDS             13779..14504
                     /locus_tag="ACFM4S_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6050473.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14539..15414
                     /locus_tag="ACFM4S_000747"
     CDS             14539..15414
                     /locus_tag="ACFM4S_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6050474.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15491..16405
                     /locus_tag="ACFM4S_000748"
     CDS             15491..16405
                     /locus_tag="ACFM4S_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC6050475.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4290 a         5277 c         3603 g         3280 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg acaaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcaccgtcg gcatcaatgt
      301 tgcctggtgc ggcatctgcg gtaccgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtca cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgagcctt acatcgtaca cgacgacgta ccgaccggcc cgggcagcaa
      541 ctaccacctc tctaaagaca tgaacttcat cggcttgggc ggctgcggag gtggcttgtc
      601 tgaaaaaatc gctgtcaaac gccgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagtaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaagtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagaact
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg agcctttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc taatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcacaagg gcgaggacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcatcg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa gtggtttgtt tcaaagacat caaccccgcc gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agctcctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gcaaaggcgg cggacaagag gttttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggcacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac tcaaatcgaa ctggaagaac taaggaaggc caagcaggaa
     2581 tttgaagctg ccgctgctca ggttcgagac agcctcaaag aaaccggtac ggatatgcag
     2641 aacagtctgc acgacatttc cgacggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgctg atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc ggcaaacacc
     2761 ctatcagacg gcatttccga cgttatgccg tctgaacgtt ccgatacttc cgccgaaacc
     2821 cttgggaacg acgggcaaat cggcggcaca gccgagcctt cggaaaccga taaagaccgc
     2881 gcatggcgcg aatatctgac cgcaccttct tcgcctgccg tacagaccgt cgaagtcagc
     2941 tatatcgata ccgctgttga aacccctgtt ccgcatacca cttcgctgcg taaacaggca
     3001 ataagccgca aacgcgattt gcgtcccaaa tcccgcgcca aacctaaatt gcgcgtccgt
     3061 aaatcataaa gagggcaaat ccggtgtccg aaacacaaaa cgaacaaccc gtccaaccgc
     3121 ttgtcgagca tctcatcgag ctgcgccgcc gcctgatgtg gacggttgtc ggcatcttag
     3181 tctgcttttt cggcatgatg ccgtttgccc aacaactcta tacttttatc gccgacccgc
     3241 tgatggcaaa cctgcccaaa gacaccagca tgattgccac cgatgtcatc gcaccatttt
     3301 tcgtgccggt caaagttacc ctgatggcgg catttttaat ttcgctgccg catacgctct
     3361 accaaatctg ggcatttgtc gcgcccgcac tctaccaaaa cgaaaaacgc ctgattacgc
     3421 cgctcgtcct ctccagcgtc agcctgtttt tcatcggcat ggcatttgcc tactttttgg
     3481 ttttccccgt cattttcaaa ttccttgcca gcgttacccc tgtcggtgtc aatatggcga
     3541 cagacatcga caaatacctc tccttcatct tggggatgtt tgtcgcattc ggtacaacgt
     3601 ttgaagtccc cattgtcgtt atcctgttaa ccaaaattgg tgtggtaaca accggacagc
     3661 ttaaacatgc ccgtccctat gttattgtcg gtgcttttgt cattgccgca gtcatcacac
     3721 cgcccgacgt tatttcacaa accctgcttg ccattccact gattctctta tacgaagcag
     3781 gtatttggtt cggacgcttc ttcactccac gttcagaaca ggatggcgac atacggccgc
     3841 ctgcaaaaac ctgacactat gccgtctgaa gccccgcctc ataccgccac agattaagga
     3901 atacctttga ataccctcta tttaggttca aacagcccac gcagaatgga aatcctgacg
     3961 cagttgggct atcgggtcgt caagctgcct gccgacatcg acgaaacggt cagacagaac
     4021 gaagaccctg cccgttacgt tcaaaggatg gcagaagaaa aaaaccgaac cgccctgacc
     4081 ctcttttgcg aaaccaacgg cacaatgccc gatttccccc tgattaccgc cgacacctgc
     4141 gtcgtttcag acggcatcat attgggcaaa ccccactccc aagccgaagc aatcgaattt
     4201 ttaaaccgat tgtccggcaa acaacatacc gtcctgactg ccgtctgcat tcattatcgc
     4261 ggcaatgcgg aaaaccgcgt ccaaaccaac cgcgtcgttt tcaagcccct gagttcggaa
     4321 gaaatttccg cctatgtgca aagcggcgaa ccgatggaca aagccggtgc ctacgccgta
     4381 caaggcatag gcggcatctt tatccaatct atcggaggca gcttcagcgg cattatgggg
     4441 ctgcccgttt atgaaaccgt ttcgatgttg caggatttgg gataccgtac cccacctttc
     4501 atccgagcct aacccgtaaa gaccgccgtg aacagacaaa ccgcttacct ccttacctct
     4561 ttcagcctga tcgcactgat aatcctgtcc ctttcctggg aactgtggat tgcaccgtta
     4621 cgcccgggcg gctcgtggct cgcgctcaaa gccctccccc tctgtctgcc gctttcaggc
     4681 atcttgaaaa agaaaatcta tacctaccaa tacagctcca tgctggttct gatttatttt
     4741 gccgaagccg tcatgcgcct gttcgacgcc tatccggcag aacaaatttg cgccgcgctt
     4801 tccgcagtat tcagcatcat cttcttcata tcctgcctgt cgttcgtcaa acaatacaag
     4861 gaaacaaaca atgcccgctg aaacgaccgt atccggcgcg caccccgccg ccaaactgcc
     4921 gatttacatc ctgccctgct tcctttggat aggcatcgtc ccctttacct tcgcgctcaa
     4981 actgaaaccg tcgcccgact tttaccacga tgccgccgca gccggactga ttgtcctgtt
     5041 gttcctcacg gcaggaaaaa aactgtttga tgtcaaaatc ccacctatca gcttccttct
     5101 gtttgcaatg gcggcgtttt ggtatcttca ggcacgcctg atgaacctga tttaccccgg
     5161 tatgaacgac atcgtctctt ggattttcat cttgctcgcc gtcagcgcgt gggcctgcaa
     5221 gagtttggtc gcacacttcg gacaagaacg catcgtgacc ctgtttgcct ggtcgctgct
     5281 tatcggctcc ctgcttcaat cctgcatcgt cgtcatccag tttgccggct gggaagacac
     5341 ccccctgctt caaaacctca tcgtttacag cgggcaaggc gtaatcggac acatcggaca
     5401 gcgcaacaac ctcggacact acctgatgtg gggcatactc gccgccgcct acctcaacgg
     5461 acaacgaaaa atcccgcccg ccttgggcgc aatctgcctg attatgcaga ccgccgtttt
     5521 aggtttggtc aactcgcgca ccatcttgac ctacatagcc gccatcgccc tcatccttcc
     5581 cttctggtat ttccgttcgg acaaatccaa caggcggacg atgctcggca tagccgcagc
     5641 cgtactccta accgcgctgt tccaattttc catgaacacc attctggaaa cctttaccgg
     5701 catccgctac gaaactgccg tcgaacgcgt cgccaacggc ggtttcacag acttgccgcg
     5761 ccaaatcgaa tggaataaag cccttgccgc cttccagtcc gccccgatat tcgggcacgg
     5821 ctggaacagt tttgcccaac aaaccttcct catcaatgcc gaacagcaca acatacacga
     5881 caacctcctc agcaacttgt tcacccattc ccacaacatc gtcctccaac tccttgcaga
     5941 gatgggaatc agcggcacgc ttctagttgc cgcaaccctg ctgacgggca ttgccgggct
     6001 gctgaaacgc cccctgaccc ccgcatcgct tttcctaatc tgcacgcttg ccgtcagtat
     6061 gtgccacagt atgctcgaat atcctttatg gtatgtctat ttcctcatcc ccttcggact
     6121 gatgctcttc ctgtcccccg cagaggcttc agacggcatc gccttcaaaa aagccgccaa
     6181 tctcggcata ctgaccgcct ccgccgccat attcgcagga ttgctgcact tggactggac
     6241 atacacccgg ctggttaacg ccttttcccc cgccactgac gacagtgcca aaaccctcaa
     6301 ccggaaaatc aacgagttgc gctatatttc cgcaaacagt ccgatgctgt ccttttatgc
     6361 cgacttctcc ctcgtaaact tcgccctgcc ggaatacccc gaaacccaga cttgggcgga
     6421 agaagcaacc ctcaaatcac taaaataccg cccccactcc gccacctacc gcatcgccct
     6481 ctacctgatg cggcaaggca aagttgcaga agcaaaacaa tggatgcggg cgacacagtc
     6541 ctattacccc tacctgatgc cccgatacgc cgacgaaatc cgcaaactgc ccgtatgggc
     6601 accgctgcta cccgaactgc tcaaagactg caaagccttc gccgccgcgc ccggtcatcc
     6661 ggaagcaaaa ccctgcaaat gacccgcgcc ggcggatgcg gataccgccc gaaatgtaaa
     6721 gctccatgca agacattgca aaaaacagca aaccggtagg gaaatacgct atcaaaaaac
     6781 attgcagccg tgttaagata aaccgtcaaa caatcttttc acgccccgcc cgaaacaggg
     6841 tcggggcata cccttacgaa aaggaaacac catgagccgc gtattactcg tagatgacga
     6901 tgccctgcta accgaactgc tgaccgaata cctgagcgcc gaaggtctga acgtccgcag
     6961 cgttcccgac ggggaagcag gcgtacagga aatcctgagc gggcagtacg atgtagtcgt
     7021 attggattcc atgatgccca aaatgaacgg cttggatgtc ttgaaaaacg tacgcgcccg
     7081 aagcaccgtc cccatcatca tgctgaccgc caaaggcgac gacatcgacc gaatcatcgg
     7141 cttggaaatg ggcgcggacg actatgtccc caaaccctgc acaccacgcg aactcttggc
     7201 acgcatcaat gccatcctgc gccgcgcaca acacagcggc gaacagaaca acgcacccaa
     7261 cagcatctcc gtcagcgatg tcgtcctgta ccccgccaaa cgccaggcat ccgtcaaaga
     7321 catgccgctc gaactgacca gcaccgaatt caacctgctc gaagtcctga tgcgccatgc
     7381 cggacaggta gtcagcaaag aaaccctgtc cgtcgaagca ctcgaccgca agctggcaaa
     7441 attcgaccgc agtatcgacg tacacatctc cagcatccgc cacaagttgg gcgatgcctc
     7501 tctgattcaa accgtacgcg gcttgggcta cctgtttgtc aaaaactgaa gcaacgggca
     7561 aatgaaactg ttccaacgca ttttcgccac attttgcgca gttatcgtct gtgcaatctt
     7621 tgtggcgagt ttttcttttt ggctggtgca gaacaccctt gccgaaaacc agttcaacca
     7681 acgccgcacc atcgaaacca ctttgatggg cagcatcatt tccgcattcc gggcacgcgg
     7741 ggacgcgggc gcgcgcgaaa tcctgacgga atggaaagac agccccgtct catcgggcgt
     7801 gtacgttata cagggcgacg agaaaaaaga tatcctgaac cggtatatcg acagctacac
     7861 catcgaacgc gcccggcttt tcgccgccgg acacccgcat tccaacctcg tccatatcga
     7921 atacgaccgc ttcggcgaag aatacctgtt cttcaccaaa gactgggaca aactccaagc
     7981 ccgccgcctg cccagccccc tgttgatccc cggtctgccg ctcgccccga tttggcacga
     8041 actcatcata ttgtccttca tcatcgtcgt cggactgctg atggcgtaca tcctcgccgg
     8101 caatatcgcc aaacccatca gaatcttagg caacggcatg gacagggtgg caaacggaga
     8161 acttgaaacc cgtatctccc aacaggtcga cgaccgcgac gacgaattgt cccatcttgc
     8221 catccaattc gacaaaatgg tggaaaaact tgaaaaactc gttgccaaag aacgccacct
     8281 gctccatcac gtctcccatg aaatgcgttc tccccttgcg cgcatgcagg caattgtcgg
     8341 actgattcag gcgcagcccc aaaaacagga gcaatatctc aaacggctgg aaggcgaact
     8401 gacccgcatg gatacgctgg ccggggaact gttaaccctg tcccgtctcg aaacttccaa
     8461 tatggctttg gaaaaagaaa gcctgaaact cctgcccttc ctgggcaacc tggtagaaga
     8521 caatcaaagc attgcccaga aaaacggaca aacggttacc ctgtctgccg acggaaaaat
     8581 ccccgaaaat acaaccatcc ttgccaacga aagctacctg taccgcgcct tcgacaacgt
     8641 catccgcaac gccgtcaact acagtcccga aggcagcacc atcctgatca acatcggaca
     8701 agaccacaaa cactggataa tcgacgttac cgacaacggc cccggcgtgg acgaaatgca
     8761 gctcccgcac atcttcaccg ctttctaccg tgcagactcc agtgcgaaca aacccggaac
     8821 aggactgggg cttgcattga cccaacatat tattgaacag cactgcggaa aaatcatcgc
     8881 cgaaaacatc aaaccgaacg gtctgcggat gcgcttcatc ctgcccaaga aaaaaaccgg
     8941 ttccaaaaca gaaaaaagtg cgaactgacc ataatgccgt ctgaaccggc ttcagacggc
     9001 attgcacaaa cagttacccc aaaacaaaca cacagcgggt gcatctccaa caatcacgca
     9061 cataccgtcc gcaaaggaat atatcatgtc ggcacaaacc gatccgggct actttttcat
     9121 gcccaaccac attattctga taggcgcgag cgaacgtccg tacagcctgg gcgaacgcgt
     9181 actcagcaac ctgctgagta cgccctttca aggaaaaatc acccccgtaa acccgcgcca
     9241 ccacaccata gccgggcttc ccgcctacac cagcctcaac aaaatccccg gcaatgcaga
     9301 cctgattatt gccgttaccc tacccgacag ttacgacacc ctcttcaaaa cctgccgcaa
     9361 aaagcagctc cgacacatca tcctcataca ggactgggac agcctgtctg ccgcagaact
     9421 gcacaccgcc gaaactgcca tccgcaaaca ccacggcaac ggacttaaca tcaccgcctg
     9481 caccaccgca ggcatccaac tgccctcact cggactcaac atcagtaccc aagacggata
     9541 cgccgcaggc cataccgcca tactgaccgg caatgccgcc gtcagccgtc aaatcgacaa
     9601 catcctgaac aaactccgtc aaggcacatc ccgccacatc agcctgcatc ccggcatcag
     9661 ccccatcaca tccgccgatt ggctcaaccg cttcggacac agcctgcaca ccaaaaccgc
     9721 cgtcctacac cacaaccctg aagaagacca gcgcaaactg ttcagcgcaa tccgccaatt
     9781 tacccgccat acgccgctga ttctccacat cacctgccgc acgacagaaa ccgaccgtgc
     9841 cgtactgcac agcctcgccc gacactgcaa cttcctcatc agtttcaacg ccgacgacct
     9901 cgaagccgca ctgcgcgccc aactgtccga ccttcctccc ctgtcccgac tcgacatcct
     9961 gtccgacacg cctgccgaat ggctgaacgc gcacgcgcca aaaaacctcg ccctccactt
    10021 tcccaacctt ccccaccaca tccgcaacgg acacctgacc ggcacaccca caccctcaat
    10081 ctgccacgac atcgcctcac gtcagcttgc ccgccccgac acccaagccg tcctaaccat
    10141 cctcagtccc tccggacacg aggattacaa aaaaacagca cgcgccctta tccgcctgtc
    10201 cgaacagacc gccaaacccc tgctcgtcag cagccccttt tcagacggca taacacattt
    10261 cgacaccccc actcaggcaa tccgcaccct ttcctaccgc aacaccgccg ccgccctgaa
    10321 acaggcacag ctcgacattg caccgccgca gccatgccgt ctgaaaacac cgcaacccca
    10381 aaacatcaaa aaagcccttg cagcggcaaa cccctccctg ctcgccgaag ccctgcacct
    10441 ccccccctac cggcacacca cccataacgc cgtacaattc caattcgaca gccaccccct
    10501 ctatggcgac atcctgaccg cacgctgcaa cggacaaacc actgccgtac tcccgccgtt
    10561 taccacgctc gacagccgcc accttgcccg ctttgccgaa ctcgacggca cacaaaccct
    10621 cgaccagttc ctgcacacac tgaccatcat tcccgaatac cgccaacaca ttctcggcat
    10681 caccctcaac ctaaacggcg gacaatacag cagcgatttc atcttaaaga cacccgaaac
    10741 acacgacacg cccaaacgca agaacacagg caaagccgcc caaaccctcg aacatgccgc
    10801 cgcaaaaatg cagagtgccg ccgcatacct gaaacacaaa aacccgacag ccgccgaatt
    10861 tctccgccac acaagcgaag ccgccgcaga actgctcggc agcaaaaccg aaaccggagc
    10921 agccgtaccc aacgtacttg ccccctatcc cgcagcatac cccaaaacac tgtccctaag
    10981 aaacaacacg accgttacca ttacccccat tttgcccgaa gacgcagaag ccaaacagca
    11041 gttcgtccgc agcctcggtc ccgaagcacg gtacacacgc ttcatgaccc acaccaacga
    11101 actgcccgca gccacgttgg cacgcctgtg caaccccgat taccactgtg aagccgcatg
    11161 gacggcaaag gatgccgaca gcaacatcgt cgccgtcgtc cgccacagcc gcctgaatcg
    11221 caacgaatgc gaattcggca tcacactggc ggaacatatg cgcggcagcg gactggcaca
    11281 gaaaatgatg gaactcatca tccaaaccgc cgcacagcaa ggctaccgga ctatgagtgc
    11341 cgacattctc aaaaccaata cccccatgat caaacttgct gaaaaatcag gatttaccct
    11401 caaggaatcg gacaccgaaa aaaacctgta ccgcgcatat ctgaaccttg cggcagacaa
    11461 aacaacagaa aaaacaaata aaaacttgcg caccgaccac aaaataacct aaaatcggca
    11521 gtttccatat atccgatttt ctcaaaagga ctcaaaatgg tagttatccg tttggcacgc
    11581 ggcggctcga aacaccgccc cttctacaac gtcatcgtta ctgactcacg cagccgccgc
    11641 gacggccgct tcatcgaacg cgtaggcttc tacaaccccg tagccaacga aaaacaagag
    11701 cgcgtccgcc tcaatgcaga ccgcctgaac cactggattg cacaaggcgc gcaagtcagc
    11761 gactccgttg caaaactgat taaagaacaa aaagccgcct aatccgcatt tgccgccatg
    11821 acagacactc aaaaccgggt agccatgggc tacatcaaag gcgtattcgg cataaaaggc
    11881 tggttgaaaa ttgccgccaa caccgaatat tccgacagcc ttttggacta ccccgagtgg
    11941 catttggtca aggacggcaa aaccatcagc gttacccttg aagccggaaa agtcgtcaac
    12001 agcgaactcc aagtcaaatt cgaaggcata aacgaccgcg acttggcatt ctcattgcac
    12061 ggttacacca tcgaaatacc ccgtgaagca ttcgccccga cagaagaaga cgaatactac
    12121 tggacagact tggtcggcat gaccgtcgtc aacaaagacc ataccgtttt aggcaaggta
    12181 agcaacctga tggaaaccgg cgcaaacgac gtattgatga ttgacggaga acacgggcag
    12241 attctgattc cgttcgtttc ccaatatatc gaaaccgtcg ataccggcag caagattatt
    12301 actgccgact ggggtttgga ctactgatgc ttatccaggc agttaccatt ttccccgaaa
    12361 tgttcgacag cattacccgc tacggcgtaa cgggacgcgc gaacagacag ggaatctggc
    12421 agtttgaagc agtcaatccc cgaaagtttg ccgacaacag attgagctac atcgacgacc
    12481 gcccgttcgg cggcggcccg ggaatgatta tgatggctcc gccgcttcat gcagcaatag
    12541 aacacgccaa agcacaatct tcccaaaccg caaaagtcat ctacctcagc ccccaaggaa
    12601 aaccgctgac acaccaaaaa gcggcagaac tggcagaact tccgcatctg attctgctgt
    12661 gcggacgcta tgagggaata gacgaaagac tgctgcaaac cagcgtcgat gaagaaatca
    12721 gcatcggaga cttcgttgtt tccggcggag agcttcccgc catgatgctg atggatgcag
    12781 tattgaggct cgtacccggc gtattgggcg atatgcagtc tgccgaacag gattcgttct
    12841 caagcggcat tttggactgc ccccactaca ccaaaccctt agaatttcaa ggtatggctg
    12901 ttccggaagt attgcgttcc ggcaatcatg gcttgatagc ggaatggcgg ttggaacaat
    12961 cgctgcgccg caccctggag cgcagacccg acttattgga aaagcgcagt ttaatcccaa
    13021 aggaatcccg cctcttggaa accatccggc aagagcaacg ggaaatccaa tcataattta
    13081 ggaaaaaaac aatgaacctg attcaacagc tcgagcaaga agaaattgtc cgcctgaaca
    13141 aagaaattcc cgaattcgca ccgggcgaca ccgtagtcgt atccgtacgc gtcgtggaag
    13201 gtacccgcag ccgtctgcaa gcctacgaag gcgttgtcat tgcacgccgc aaccgtggtc
    13261 tgaacagcaa cttcatcgtc cgcaaaatct ccagcggcga aggtgttgaa cgtactttcc
    13321 aactgtactc tccgaccgtc gaaaaaatcg aagtcaaacg ccgtggtgac gtacgccgtg
    13381 ccaaactgta ctacctgcgc ggcctgaccg gcaaagctgc acgcatcaaa gaaaaactgc
    13441 ctgcacgcaa aggttgattc aaacccgttt tccccaatgc cgtctgaacc ttctgacggc
    13501 atttcttatt gccgcaacat ccgcaacgga acagcaccaa gaagcacacc ccttccggca
    13561 catcccgaat ccccgtccga accgaacaaa atttacagac caaacgttat acagtatcat
    13621 atcagccata taataataac cgctttaccg ctttcccttt caaaaccgca aatcatcatg
    13681 acaccttccc ttttactatc aggattgacc ttccgcctcg tccttgccct gattgccgta
    13741 tcccttttat ggggcgttta cttttgggca gtatccgcat gagcatcatt gtcgaaaacc
    13801 tgaccgtcag ctaccgccgc cgtcctgccg tacaccacgt tgacattact tttgaagaac
    13861 atagtatgtg ggcggttttc ggtcccaacg gcgcagggaa atccaccttt ctcaaatcct
    13921 tgatgggatt gcagcctatc gatacaggca gcatccggct ggatggattg agccgccaag
    13981 acatcgccta ccttccccag cagtccgata tcgaccgctc ccaacctatg actgttttcg
    14041 acttggcggc aatggggctt tggtatgaaa tcggcttttt caaagggata aataccgctc
    14101 aaaaacaacg cgttcacgaa gccttggagc gtgtaggaat gcggcagttt gcacgccgcc
    14161 aaatcgccca cctctcaaac ggacaattcc aacgcgtcct ttttgcccga atgctggttc
    14221 aaaatgcaaa attcctgctt ttggacgaac ctttcaacgc cgttgatgca cggacgacct
    14281 acgaacttct cgacgtattg cagaaatgcc attgcggcgg acacgctatc attgccgtac
    14341 tgcacgatta cgaacaagtc cgtgcctact ttcccagtac cctgctgctc gcccgagaaa
    14401 aaattgcggc aggcgcaacc gagaccattc tgacagaacc ctacctcgcc caagccaacg
    14461 ccaaaatgca gcaacaggaa agccccgact ggtgcgcctc ataaatactg cccgaaacca
    14521 cgccccacac cagacaccat gaatctctac gacctgctcc ttgccccctt tgcagaattc
    14581 gactttatgc gctacgccct cgcatccgtc ttctgcctgt ccctcagtgc cgcacccgtc
    14641 ggcgtattcc tcgtcatgcg ccgtatgagc ctgataggcg acgcattgag ccacgccgtc
    14701 ctgcccggtg ccgccgtcgg ctacatgttt gccggcttga gcctgcccgc catgggtttg
    14761 ggcggcgtag ccgcaggcat gctgatggca ctgcttgccg gactcgtcag ccgcttcacc
    14821 accctgaaag aagatgccaa ctttgctgcc ttttatctca gcagcctcgc catcggcgta
    14881 gtcctcgtca gcaaaaacgg gagcagcgtc gatttgctcc acctcctttt cggctctgta
    14941 cttgccgtcg atattcctgc cctgcagctc atcgccgccg tctccagcct cacgctcatt
    15001 acccttgccg tcatctaccg cccgctcgta ctcgaaagca tcgaccccct gtttctcaaa
    15061 tccgtcggcg gcaaaggcgg gctttggcac gtcctctttc tcgtcctggt cgtcatgaac
    15121 ctcgtatccg gctttcaagc cctcggcaca ctcatgtccg tcggactcat gatgctgcca
    15181 gccattaccg cccgcctgtg ggcgaagcac atgggcgcac tcatcctcct atccgttctg
    15241 acagccctgc tgtgcggctt gagcggactg ctcatttcct accacatcga aattccctcc
    15301 ggtcccgcca tcatcctctg ttgcagcgtc ctttatctct tttccgtcat actcggcaaa
    15361 gaaggcggca ttctgaccaa atggctcaaa aaccaccgcc accacaccac ctgaccccgc
    15421 aagccgcttg aacaaacagg cagcttgttt taccataaca gatgttacgt tatatcacag
    15481 gagaacagtt atgaaacacc tcaaactcac ccttattgcc gcattgctaa ccaccgccgc
    15541 aactgccgcc cccctgccgg ttgtaaccag cttcagcatt ttaggcgacg tagccaaaca
    15601 aatcggcgga gagcgcgtat ccatacaaag tttggtcgga gccaaccaag atacgcacgc
    15661 ctatcatatg accagtggcg acattaaaaa aatccgcagt gcaaaactcg tcctgctcaa
    15721 cggcttagga cttgaagctg ccgatgtaca acgtgccgtc aaacagagca aagtatccta
    15781 tgccgaagcg accaaaggca tccaacccct caaagccgaa gaagaaggcg gacaccatca
    15841 cgaccacgat catgaccacg aaggacacca ccatgaccac ggcgaatatg acccccacgt
    15901 ctggaacgac cccgtcctta tgtccgccta tgcccaaaac gttgccgaag ccctgataaa
    15961 ggccgatccc gaaggcaaag tttattatca acaacgcttg ggcaactacc aaatgcagct
    16021 taaaaaactg cacagcgacg cacaagccgc atttaacgcc gtccctgccg ccaaacgcaa
    16081 agtcctgacc gggcacgatg ccttttccta tatgggcaaa cgttaccata tcgaattcat
    16141 cgccccgcaa ggcgtgagca gcgaagccga gccttcagcc aaacaagtcg ccgccatcat
    16201 ccgccaaatc aaacgcgaag gcatcaaagc cgtatttacc gaaaacatca aagacacccg
    16261 catggttgac cgtatcgcca aagaaaccgg tgtcaacgtc agcggcaaac tgtattccga
    16321 cgcactcggc aacgcgcccg cagacaccta catcggaatg taccgccaca acatcaaagc
    16381 cttgaccaac gcgatgaagc aataaaaccg tttgattggt ttgaatttga aaaggcaaaa
    16441 ggccgtctga
//
LOCUS       DAXHMX010000031        16094 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHMX010000031 DAXHMX010000000
VERSION     DAXHMX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16094
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.031"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(37..762)
                     /gene="aat"
                     /locus_tag="ACFM4S_000749"
     CDS             complement(37..762)
                     /gene="aat"
                     /locus_tag="ACFM4S_000749"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6050476.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFLRDGWFFWYAVGPRAVIVPERLHVPRSLEKTLRNCSYRVTVNGCFAEVVAHC
                     AAAVRPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLASLGVELIDCQQDTGHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(832..1266)
                     /gene="fur"
                     /locus_tag="ACFM4S_000750"
     CDS             complement(832..1266)
                     /gene="fur"
                     /locus_tag="ACFM4S_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6050477.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1493..1870
                     /locus_tag="ACFM4S_000751"
     CDS             1493..1870
                     /locus_tag="ACFM4S_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6050478.1"
                     /translation="MNKTLILVLSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPMLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1880..2689
                     /gene="dapB"
                     /locus_tag="ACFM4S_000752"
     CDS             1880..2689
                     /gene="dapB"
                     /locus_tag="ACFM4S_000752"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6050479.1"
                     /translation="MTPLKIAIAGVNGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2803..3213)
                     /locus_tag="ACFM4S_000753"
     CDS             complement(2803..3213)
                     /locus_tag="ACFM4S_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050480.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFVYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LELIGGRYDFPTAFRIDIVCREIELEFLDQEGFN"
     gene            complement(3215..3448)
                     /locus_tag="ACFM4S_000754"
     CDS             complement(3215..3448)
                     /locus_tag="ACFM4S_000754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050481.1"
                     /translation="MKQHVLQKKGGEIVINGTSNNKYLRGIPNEGELARVGLRLKYNG
                     PLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(3469..4623)
                     /gene="lpxB"
                     /locus_tag="ACFM4S_000755"
     CDS             complement(3469..4623)
                     /gene="lpxB"
                     /locus_tag="ACFM4S_000755"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6050482.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRERCPQARFTGIGGE
                     LMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAEKLKRTGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATAATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQHDAV
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4689..5681)
                     /locus_tag="ACFM4S_000756"
     CDS             complement(4689..5681)
                     /locus_tag="ACFM4S_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6050483.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKVGLPPDLAQFAVMLENGTKM"
     gene            6174..8933
                     /locus_tag="ACFM4S_000757"
     CDS             6174..8933
                     /locus_tag="ACFM4S_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6050484.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEMQTGKRRRNGSRSE
                     RSQTAPETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
     gene            complement(9883..10872)
                     /gene="pdxA"
                     /locus_tag="ACFM4S_000758"
     CDS             complement(9883..10872)
                     /gene="pdxA"
                     /locus_tag="ACFM4S_000758"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6050485.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            11032..12459
                     /locus_tag="ACFM4S_000759"
     CDS             11032..12459
                     /locus_tag="ACFM4S_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6050486.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            12784..14679
                     /gene="mnmG"
                     /locus_tag="ACFM4S_000760"
     CDS             12784..14679
                     /gene="mnmG"
                     /locus_tag="ACFM4S_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6050487.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            14747..15331
                     /gene="rnhB"
                     /locus_tag="ACFM4S_000761"
     CDS             14747..15331
                     /gene="rnhB"
                     /locus_tag="ACFM4S_000761"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6050488.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRHDFAPVRNLLAQQTLF"
     gene            15344..15589
                     /locus_tag="ACFM4S_000762"
     CDS             15344..15589
                     /locus_tag="ACFM4S_000762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050489.1"
                     /translation="MPSELQTAFPASQLQRERTHTAIPAKNRKSKKKPKIRHSRAGGN
                     PIRPVSVFFEFQVTSKPPFPQKRESRNSKLQEFIKND"
     gene            complement(15907..>16094)
                     /locus_tag="ACFM4S_000763"
     CDS             complement(15907..>16094)
                     /locus_tag="ACFM4S_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050490.1"
                     /translation="IFVNPLYRHSRKNKKIKNTKLKYRHSRAGGNLGLSVRKLIGKNG
                     FSNPETPDSCFRGNPVF"
BASE COUNT         3988 a         4912 c         3950 g         3244 t
ORIGIN      
        1 caccatattt gaacctgacc gaagcataag ccgccatcaa agccccttgc acgccacttc
       61 gcgccgcccg atttcctctt tcaacggcac ggcgttcaac atccgcagac gttcggcaaa
      121 atccgcaaac ggcagcagct ccgaaccgaa acggcgcata tgccccgtat cctgctggca
      181 gtctatcagt tccacaccca aagacgcgag aaacggcacg gcgcaggcaa acgcgatttt
      241 cgacgcatcc ggttgtaatg cgaacatcga ttcgccgtaa aacaccctgc cgatctgaac
      301 gccgtaaaac ccgcccgcca acctcgtttt accgttttca tcgggataat ggcactcaaa
      361 cgaatgcgcg tatcccattt cgtgcagctt caaatatgcc gtctgaaact cgggcgcaat
      421 ccaagttccg tcctgattcg ggcgcaccgc tgccgcacaa tgcgcgacca cttccgcaaa
      481 acagccgttg accgtaaccc gatagctgca gttgcgtagc gttttctcca gcgagcgcgg
      541 aacgtgcaga cgttcgggaa caatcaccgc acgcggcccg accgcatacc aaaaaaacca
      601 accgtctcgc aaaaaccaag gaaacacgcc gttccgatac gcctcgagca gccgccccgc
      661 gtccaaatcc ctgctcacgc cgaccagccc gtcgcaccgt gccaaagcat aggcaggatc
      721 gggaaaggca taattgtcag gggcaagcag cggaatacgc atggcagttc tccaaaaaca
      781 cagacggcat ccaaaatcat gaatgccgtc tgaacaacaa accgtccgga tttaacgttt
      841 gcccttggcc tgacagtcgc tgcacacgcc gtacatataa agcgcgtgat cgacgatgcg
      901 gtagccgttt tcctcggcga ttttgtcttg cagggcttcg atttcgggat tgtggaattc
      961 cgttacctcg ccgcacttca cacagacgat atggtcgtgg tggtcgccct tgtccaactc
     1021 ataaaccgcc ttgcccgttt caaaatggtg gcgttgcaaa atgcccgcct gctcaaattg
     1081 cgtcagcacg cgataaatcg tcgccacacc gatttccaca ccctcttcca acaaaatacg
     1141 gtacacatcc tcggcactca aatgctcttc cgcatgagtc tcgaacaaat ccaaaatctt
     1201 caaacgcggg ccggtaactt tcagtccact gtctttcagt tgcgcaatgt tgctgaattt
     1261 ttccataata ttcaatatcc ctgtaaaata atagacgcta taatacgcaa tttcagcctg
     1321 cttgcccact atcacaccat agcagtttgc aatagcaaaa ccgcaacggc ggacgggcag
     1381 tcggacacag acagggtgaa ataccgctta agcgtatgat tatcgttcgc ttttataaaa
     1441 tattcaagca gtgctacact agacatcccg atttgcacag aaaggttctc ccgtgaacaa
     1501 aaccctcata ctcgtccttt ccgccctgtt cagcctgacc gcgtgcagcg tcgaacgcgt
     1561 ttcactgttc ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccccgcgc
     1621 cgttgccgcc ctgcgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc
     1681 catgctgcgc gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa
     1741 cggcatcatc aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg
     1801 caccgaaggc gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga
     1861 caaaccataa ggaacacaca tgacaccgct caaaatcgct atcgccggcg tcaacggccg
     1921 catggggcgc gtattggttg aagccgtcaa caaccatccc gacaccgtcc tttccggcgc
     1981 gctcgaacac tcgggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa
     2041 aaccggcatc gccatttcag acgacgttga cgccgttctc gcacaaagcg acgtactcat
     2101 cgacttcacc cgccccgagc ccaccctcaa acacctgcaa aaatgcgttg aaaaacaagt
     2161 caacatcatc atcggcacaa caggcttcga cgatgcgggc aaagccgcta tccacactgc
     2221 cgccgaacaa acaggcatcg ttttcgccgc caacttcagc gtcggcgtca acctcacctt
     2281 ccacatcctc gacaccgtcg cacgcgtatt aaacgaaggc tacgacatcg aaatcattga
     2341 aggccaccac cgccacaaag tcgatgcccc cagcggcacc gcgttacgca tgggcgaagt
     2401 catcgccggc gcgctcggca gagacctcaa acaatgcgcc gtttacggcc gcgaaggcca
     2461 caccggtccg cgcgatccgt cgaccatcgg ctttgccacc gtccgcgcag gcgacatcgt
     2521 cggcgaccac accgccctct tcgccaccga cggcgagcgc gtggaaatca cccacaaggc
     2581 cagcagccgc atgacctttg ccgccggtgc cgtccgcgcc gcagtttggg taaacggcaa
     2641 aacgggtttg tacgatatgc aggacgtact cgggctgaac aaccgttaac ccccatacaa
     2701 aatgccgtct gaaaagatat tgttcagacg gcattttgcc aacaggctcc gtatcggcat
     2761 atcaataaag ttgcttgcgc ggcatgacaa gctacggctc gtttaattga aaccctcctg
     2821 atctaaaaat tctaactcta tttcccggca aactatatct atcctaaaag cagtcggaaa
     2881 atcataccta ccgcctatca attccagcgc acctccataa tccaagattt catcatcctg
     2941 ctttaattcg ccaagttcca atgtattatc gatatttata tcttggctct ttgtagcgta
     3001 tccatagaca aatttcagat agctgaactg cttgaattta atttttaccc agtctgtata
     3061 atttggtgta ggaatctctt gaggaaagtc cggataatcc acattctgga cgatgatatg
     3121 gaattcatcg tttttcaaag tatagtttaa tagagtgccc tcatatatat acagaaattg
     3181 ataatctttt tttggaatgg tataagttgt catattattt taccttttca aaaacaatga
     3241 gtttaagatt ttttgagtcc aagttggttc tactactctt atgaaatttc agttgtttaa
     3301 actcttcctt taacggccca ttatatttta accttaatcc cactcgagcc agttctcctt
     3361 catttggaat tcctcttaaa tacttattgt ttgatgtgcc attaatcaca atttctcccc
     3421 cctttttttg taatacgtgc tgcttcaaga agataatcca ttaaccgctc aacatcccgc
     3481 ctcttccaaa accgcgcgcg cggccaaatc cgccgtatct tttttcaaca gcaggtgcaa
     3541 cgccctgaaa tcctgttgca gcgcggcaac cttatcgggg tgttcgtacc agtccgccaa
     3601 cgccgccgcc agtttttccg gtacggcatc atgttgaagc agctccggca cagcctcctt
     3661 acccaacagg atattcggca ggccgacatg cggcactttg attttgcgtt tcacataagc
     3721 ataagtcagc ggcgaaatct tgtagctgat gaccatcgga cgcttgcaca gagccacttc
     3781 caaagttgcc gtaccgctcg ttaccagcac cgcatccgcc gccctgcaca ctgtttcaga
     3841 ctgtctgtcg attaccgtca gcggcaatcc ggcaaactcc ggccgctgca aaacttccgc
     3901 caaacgccgc ttcgtcgccg ccgttgcggc aggcagcagg aagcgtgcgg caggatagcg
     3961 ttccaacaac aataatgccg tctgaaaaaa caccggcgcc atatagtcga tttcgctgac
     4021 gcggctgccg ggcagcaggg cgaatacggg gatgccggca tccacgccca aagttttccg
     4081 cgccgtttca cggtcgtctt ccaagggcat aagctgcgcc atcggatgac cgacaaactc
     4141 cgcacgtccg cccgcatcga gataaagctg cggctccatc gggaacaggc acagcacgcg
     4201 gttgacctga tgcacgattt tgcccacgcg ttcccgccgc cacgcccaca ccgacggact
     4261 gacataatgc acggtcggaa tgcccgtccg tttcagcttt tccgccacac ccaaattgaa
     4321 atcgggcgca tcgataccga caaagacatc aggtttcaac gacagcaaat cccgtaccag
     4381 ccccctgcgt atccgtaaaa tttccggcag ccgcctgacc acttcgacaa agccgcgcac
     4441 cgccagccgc tcctgatcat aaaggctctc gaaaccttcc gccttcatca gttccccgcc
     4501 gataccggta aaccgcgcct gcgggcaacg ctcgcggatg gcgcgtatca ggtgcgcccc
     4561 caacaggtcg cccgacgctt cgccgacact gacggcaatc aaagggcttt ttttatcaac
     4621 catattcgtt cttcttcaca tactttcacg tcccgatgcc gtctgaaggc ttcagacggc
     4681 atcggggttc acattttcgt cccgttttcc aacatcaccg caaactgtgc caaatccggc
     4741 ggcagcccca cctttaacac cagcggctcg cccgtgagcg gatggttcag gtgcagctcg
     4801 gacgcgtgca aaaacatccg cttcaaaccc aacttctgca aacgacggtt cgcctgataa
     4861 tcgccgtagc gttcgtcgcc cgcaatcgga cagccttgag attgcaggtg gacgcggatt
     4921 tggtgcgtgc gccccgtttt caacgtcgcc cgcaccaaag tcaggtgcga cagcccgaca
     4981 ccgtgcaaaa tgccgtctga aaaacggctt aacacacgga acaccgtatg cgccgactgc
     5041 ccgtccgcac tgacgcgcac catcttttcg ccttgtgcgc cggtatattt gaacaggggc
     5101 agtttgacat ggaaattgtc gtccggcagt ttgcccaccc ccagcgcaag gtagattttt
     5161 ttggggtggt cgttacggat ggcttcgtga agtttgacga gcgcgctgcg tttcttcgcc
     5221 accatcaaca agccgctcgt atccttgtcc aaacgatgaa ccaactccaa atacttcgcc
     5281 tccggacggg cgcggcgcaa ctgttcgata acgccgaaac tcacgccgct gccgccgtgg
     5341 acggcaacgc cggacggttt gtcgatgacc aaaagcgcat cgtcttcgta aacaacgtca
     5401 aacgcacgcg ccggtacggc ggcacgcctt tcagacggca tttccttctc cgccacgcgc
     5461 acaggcggaa tccgcaccgt atccccctcc gcaatacggc tgtcgggttt gcagcgtttc
     5521 ttgttcaacc gcacctcgcc ggcgcggata atgcggtgga tatggctctt gggaacaccc
     5581 ttgaggattt ttatcagata gttatcaagg cgttgacccg cctcatgttc ggcaacccct
     5641 atcaagctga ccgaatcttt gcttatttcg tgcgttttca tctataatcc gaacatccgt
     5701 ttcagcaaaa agcgcgcccg ccgcgcctcc tgaaacggtt tactttaaaa cggaatttta
     5761 tattaaaaac gcactccgca aagcattttc cctgcgcctg ttcccagccg gcaggcgcag
     5821 aacgtaatca agtttgaatt gattttgccg tttcggcgcg gaagtaagac ggcagccggg
     5881 cccaagtccc aaacgcaaac cgcccaaaac acaggcatca cgataacaag aagaagccgg
     5941 cccccatttt tccgaccgca gcgttcggaa cgcggcagac aaaccgccgc atcgatcttt
     6001 atagccttct gtatccgacc cttccgcaca cggttctccg gaacgtgcat ccctgcggat
     6061 tttcaactca aaaactgatt accggtttta tccgaaaaca tcggaaaacc cagccttacg
     6121 gaatgccgaa ccgggcatgt gccgtctgaa cgccgcctgc ccacgaggtg atcatgaaaa
     6181 gaatgttatt taacgcaacg caggccgaag agctgcgcgt tgccatcgtc gacggacaaa
     6241 acctgctgga tttggacatc gaaacgctgg gcaaagaaca gcgcaaaggc aatatctaca
     6301 aaggcatcat tacccgcatc gagccgtcgc tggaagcgtg tttcgtggat tacggaaccg
     6361 accgccacgg cttcttgccg tttaaagaag tctcccgctc atacttccaa gactacgaag
     6421 gcggacgcgc gcgcatccaa gacgtgctca aagaaggcat ggaagtcatc gtccaagtcg
     6481 aaaaagacga gcgcggcaac aaaggcgccg cgctgaccac cttcatcagc ctcgccggac
     6541 gctatctggt attgatgccg aacaacccgc gcggcggcgg cgtatcccgc cgtatcgaag
     6601 gtgaagagcg tcaagagttg aaagccgcca tggcggaact cgacattccg aacggcatga
     6661 gcatcatcgc ccgtaccgcc ggcatcggcc gcagcgcgga agagttggaa tgggacttga
     6721 actacctcaa acaactctgg caggcgattg aagaagcagg aaaagcgcat cacgacccct
     6781 acctgctctt tatggaaagc tcgctgctga tccgagccat ccgcgactat ttccgccccg
     6841 acatcggcga gattttggtg gacaatcaag aagtttacga ccaagttgcc gagttcatga
     6901 gctacgtcat gccgggcaat ataggccgtc tgaaactcta cgaagaccac acgccgctgt
     6961 tttcccgctt ccaaatcgaa caccaaatcg aaagcgcgtt ttcgcgcagc gtcagcctgc
     7021 cctccggcgg cgcgatcgtc atcgaccata ccgaagccct cgtctccatc gacgtgaact
     7081 ccgcacgcgc cactcgcggc gcagacattg aagacaccgc gttcaaaacc aatatggaag
     7141 ccgccgaaga agtcgcccga caaatgcgcc tgcgcgactt gggcggcttg gtcgtcatcg
     7201 acttcatcga catggaaaac cccaaacacc agcgcgatgt ggaaaacgtc ctgcgcgacg
     7261 cgctcaaaaa agaccgcgcc cgcgtgcaga tgggcaaact ctcccgtttc ggacttttag
     7321 aattgagccg ccaacgtttg aaaccggctt tgggcgaaag cagccacgtc gcctgtccgc
     7381 gctgcgccgg caccggcgtg attcggggca tcgaatccac cgccctgcac gttttacgca
     7441 tcattcaaga agaagcgatg aaggacaaca ccggagaagt gcgcgcacaa gtgcccgtcg
     7501 atgtcgccac cttcctgctg aacgaaaaac gcgccgagct gtttgcgatg gaagagcgtt
     7561 tggatgtcaa cgtcgtcctg attccgaaca tccacctcga aaatccgcac tacgaaatca
     7621 accgcatccg caccgacgac gtagaagaag acggcgaacc gagctacaaa cgcgtcgccg
     7681 agccggaaga agacgaatcc gccaaaccgt tcggcggcga aaaagccaaa gccgcccgtc
     7741 ccgaacccgc cgtcaaaggc gtgcgccaca ccagccccgc cccgactgcc gcccccgaga
     7801 aaaaaacctc ttggtgggac agcttcaaag catggctcaa acgcattttc ggcggcagcg
     7861 aaacccaagc cgcgcccgct gccgaaacct ccgaaaaacg cagcacggca aaccgcagcg
     7921 gcagccgcgc caacaaccgc cgccaaaacc cgcgccgcag caaacgcgaa ggcagcaaag
     7981 tagaagtccg cgaagtggca ggcaaaactg ccggacagga agcgcgtgcc gacaaagccg
     8041 aaacgcgcaa caacggcaac cgccgccgca atgaacgcgg cgaccgtgcc gccgaacgcg
     8101 ccaacgaagc ggaaatccaa agccgcaacg tacagcctgc cgcaaccgtt gcagatgccg
     8161 caccgtccga aaccgaaatg caaaccggca aacgccgccg caacggcagc cgcagcgaac
     8221 gcagccaaac cgcgccggaa accgccgccg ttgccgaaac agccgttcag acagcggaaa
     8281 acacgccgtc cgaaccgcat accgcagaag acaaaggcag caagcccaaa tccgaacgca
     8341 accgccgcga acgcgacagc cgcgacgcca aagaacgccg cgagcgcaac aatcagcgcg
     8401 accgccgtca aaacggcaaa aaacgcaata tcccgtctgc cgccaaaatc gagcagtacc
     8461 tgaatattca cgacaccgcc gacaaagtcc gttccgccgc cgcgcacgtt ttcggcgaaa
     8521 ccgacgcaaa cgcgccgatt accgtcagca ttgccgatcc ggttgcagaa agggatcttc
     8581 cgacagcatc tcccgccgtt tcaaacggcg acgcaccggt ttatgatgcg gcggaaaaaa
     8641 tccgccgtgc caccgccgcc atcctgcccg aaggcgcgac accgaaagcc gaagcacagg
     8701 aaatgccgtc tgaaaccgca acctttacgg ctgcggcgga acaggcacgg gaaaccgcac
     8761 aaaccggcgg actcgtcctg atcgaaaccg accctgccgc attgaaggca tgggcggcac
     8821 aacccgaagt ccaaaccgga cgcggtttgc gccgttccga acagcccaag ccgtctgaag
     8881 tcgcaaccgt ccctgccgaa gaaatgatcc aagtcgaaac ccggcaaggc tgaaccgacg
     8941 gcggcaaaaa gaggttctgt tccgcagaac ctctttttta tatagattcg gatacctgca
     9001 atgccgtctg aagcctcacc attcccatga ttaccgcaac ctttcgtcat tcccgtgaaa
     9061 acgggaatct agaacctcaa actttcaaat aacctttgaa tattgccgct gccttaaggt
     9121 ctggattccc acctgcgcgg aatgacggtt tagaagttgc ccgaaacctc aaaaaaaact
     9181 gaaaccgaac aagccggatt cccgcaacat tccgtcattc ccgtgaaaac gggaatatag
     9241 aaccccaaac tttcagataa tctttgaata ttgccgtcgc ccaaaggcct ggattcccgc
     9301 ctgcgtggga atgacggcgg agggtggacg atgccgtctg aaacttcgcc attcccatga
     9361 ttaccgcaac ctttcgtcat tcccgccacc tttcgtcatt cccgtgaaaa cgggaatata
     9421 gaacctcaaa ctttcagata atctttgaat attgccgccg cccaaaggtc tggattcccg
     9481 cctgcgcggg aatgacggcg gagggtgaac gatgccgtct gaaacttcac cattcccatg
     9541 attaccgcaa cctttcgtca ttcccgtgaa aacgggaatc tagaacctct aaactttcag
     9601 ataatctttg aatattgccg tcgcccaaag gcctggattc ccgcctgcgc gggaatgacg
     9661 gtttagaagt tgcccgaaac ctcaaaaaaa aaccgaaacc gaacaagccg gattcccgca
     9721 acattccgtc attcccgtga aaacgggaat ctagaacctc aaactttcag ataatctttg
     9781 aatattgccg ccgcccgaag gtctggattc ccgcctgcgc gggaatgacg gcggagggtg
     9841 gacgatgccg cctgcgcgga aatgacgggt cttttatcat ctttaaaggc tgccgcgcgc
     9901 catctcgacg gcggtctcca cggcagttat caggctgccg gaatccgccc tgccggttgc
     9961 cgccaaatcg agcgcggtgc cgtgatcgac agaggtgcgg ataaagggca gacccagcgt
    10021 gatgttcacg ccctgcccga aactgtggta tttcaacacg ggcagccctt ggtcgtggta
    10081 catcgccaat acggcatccg caccttcgag cataaacggc tggaacaatg tgtccgccgg
    10141 atacgggccg gcaaggttta tcccttcgcg gcgcaggttt tccaatgcag ggataatggt
    10201 gtcggtttct tcgtgtccga ggtgtccgcc ttcgccggcg tggggattaa gtccggcgac
    10261 aaggattttg ggatttttga tgccgaattt gtgttttaag tcgtgatgca aaatgcgtgc
    10321 gacgctttca atcagcggtt gcgtgatggc ggcggcaacg tctttcagcg gcaggtgggt
    10381 cgttacgagg gcgacgcgca ggcctttgcc ggcaagcatc atcacgacct gccccgtgcc
    10441 gcttttttcc gccagatatt cggtgtgtcc gctgaaaaaa cctgtgcttg cgcgcgcgtc
    10501 gttgatgatg cctttgtgca gcggcgcggt aacgatgccg tcgaaaatgc cgtctgaaat
    10561 gcctgcgagc gcggtgtcca aaagttgcag cacataggcg gcgttggcgg gattgagttt
    10621 gcccgcctca accgcttcga cggcagggat gtgcagcact tccagctcgc cgtatgccgc
    10681 gccgcctgat tctggatcga agtcgcgcag gacgacgctt ttgcccaagg cttcggcgcg
    10741 cgcgcgcaat aggtttttgt cgcccaatac cgcgcagcgg cagggcaggc gtgcaaacgc
    10801 caagtccaaa caaatatcgg ggccgatgcc ggcaggctcg ccggaagtaa cggcaaaaac
    10861 aggctgtttc atcgtgtttg ctccaaacaa aatgcgattc taacgccgca gccgcgcggc
    10921 gatgtaaatt tttctgattt tgttgacaat ctgctagaat gggcgtttac aaaatttaaa
    10981 ccctgcttgc ataccgccaa tatgtgcgag tttcaacttt aaggaagcga tatgaacgag
    11041 aactttaccg aatggctgca cggctgggtc ggcgccatca acgatccgat gtggtcatac
    11101 ttggtttata tgcttttggg tacggggctt ttcttcaccg taaccacggg ctttgtccaa
    11161 ttccgcctgt tcgggcgcag catcaaagaa atgctcggcg gccgcaaaca gggggacgac
    11221 cctcacggca tcacgccgtt tcaggcattt gtaaccggcc ttgccagccg cgtgggcgtg
    11281 ggcaatatcg cgggcgtggc catcgccatc aaagtcggcg gaccgggcgc ggtgttttgg
    11341 atgtgggtaa ccgccttaat cggtatgagt tcggcgtttg tcgaatcttc gctggcgcag
    11401 ctctttaaag tccgcgacta cgacaaccac catttccggg gcggccctgc ctactacatc
    11461 actcaagggc tggggcagaa atggctgggc gtgttgttcg ccctgagcct gattttctgt
    11521 ttcggctttg tgtttgaagc ggttcagacc aataccattg ccgataccgt caaagcggcg
    11581 tggggttggg agcctcatta tgtcggcgtc gccctggtga ttttaaccgc gccgattatc
    11641 ttcggcggca tcaggcgcat atctaaagcg gcggaaatcg tcgtccccct gatggcggtt
    11701 ttgtacctct ttatcgcgct tttcatcatt ttgaccaata ttccgatgat tccggacgtg
    11761 ttcggtcaga ttttttcggg cgcgttcaaa ttcgacgcgg cagcaggcgg cttactcggc
    11821 ggtctgattt cgcaaacgat gatgatgggc atcaaacgcg gcctgtattc caacgaggcg
    11881 ggtatgggtt ccgcgccgaa cgccgccgcc gccgccgaag tgaaacaccc tgtttcgcaa
    11941 ggtatgattc aaatgctggg cgtgtttgtc gataccatca tcgtttgttc ttgcaccgcc
    12001 ttcatcatct tgatttacca acagccttac ggcgatttga gcggtgcggc gctgacgcag
    12061 gcggcgattg tcagccaagt ggggcaatgg ggcgcgggct tcctcgccgt catcctgttt
    12121 atgtttgcct tttccaccgt tatcggcaac tatgcctatg ccgagtccaa cgtccaattc
    12181 atcaaaagcc attggctgat taccgccgtt ttccgtatgc tggttttggc gtgggtctat
    12241 ttcggcgcgg ttgccaatgt gcctttggtc tgggatatgg cggatatggc gatgggcatt
    12301 atggcgtgga tcaaccttgt cgccatcctg ctgctctcgc cactggcgtt tatgctgctg
    12361 cgcgattaca ccgccaagct gaaaatgggc aaagaccccg agttcaaact ttccgaacat
    12421 ccgggcctga aacgccgtat caaatccgac gtttggtaaa tcccgccctt accggagccg
    12481 cttccccgcg aagcggcttt tccctttccg cacactgtaa aaacagggcg aacaagcgta
    12541 caatcccaac cctttacttt tgaatccatt tcgtttttca gacggcatat tgaatatagt
    12601 ggtttaacaa aaatcgggac aaggcggcga agccgcagac agtacagata gtacagaacc
    12661 gattcacttg gtacttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac
    12721 gctgtactgg tttttgttaa tccactataa atgccgtctg aaacaccgtc aggcaataca
    12781 cacatgaccc acatgattta ccccaaaacc tacgacgtta tcgtcgtcgg cggcggacac
    12841 gccggcacag aagccgcgct cgccgccgcc cgtatgggcg cgcagacgct tttgctcaca
    12901 cacaatattg aaacgctcgg acaaatgtcg tgcaaccctt ccatcggcgg catcggcaaa
    12961 gggcatttgg tgcgcgaact cgacgcgctc ggcggcgcga tggcgttggc aaccgacaaa
    13021 tccggcatcc agttccgccg tctgaacgcc agcaaaggcg cggcagtacg ggccacgcgc
    13081 gcgcaggcgg atcgtatcct atataaagcc gccatccgcg aaatgttgga aaaccaagaa
    13141 aacctcgacc ttttccaaca agccgtcgaa gacgtaacgc tcgatggcga ccgcatttca
    13201 ggcgtaatca ccgcgatggg cgtggagttt aaagcacgcg ccgtggtgtt gaccgcaggc
    13261 acgtttttgt ccggcaaaat ccacatcggt ttggaaaact acgagggcgg acgcgccggc
    13321 gaccccgccg ccaaatcgct gggcggacgt ttgcgcgaat tgaacctgcc gcaaggccgt
    13381 ctgaaaaccg gcacgccgcc gcgcatcgac ggacgcacga ttgacttctc ccaactgacc
    13441 gaacagcccg gcgacacgcc cgttcccgtc atgtccgtac gcggcaacgc cgaaatgcac
    13501 ccgcgccaag tgtcctgctg gattacgcat accaacacgc aaacccacga catcatccgc
    13561 tcaggcttcg accgcagccc gatgtttacc ggcaaaatcg aaggcgtggg cccgcgttat
    13621 tgtccgtcta tcgaagacaa aatcaaccgc ttcgccgaca aagacagcca ccagattttc
    13681 ctagaacccg aaggcctgac tacgcacgaa tactacccca acggtatctc caccagcctg
    13741 ccgttcgaca tccaaatcgc gctcgtccgc agtatgaaag gtttggaaaa cgcccatatc
    13801 ctgcgccccg gctacgccat cgaatacgac tacttcgatc cgcgcaacct caaagcaagc
    13861 ctcgaaacca aaaccattgc cggattgttt ttcgccgggc aaatcaacgg tacgacaggt
    13921 tatgaagaag ccgccgcgca aggactgctg gcaggcgcga acgccgtgca atacgtccgc
    13981 gaacaagacc cgctcctgct gcgccgcgaa caagcctatc tcggcgtatt ggtggacgac
    14041 ctcatcacca aaggcgtgaa cgaaccctac cgaatgttca ccagccgtgc cgaataccgc
    14101 ctgcaactca gggaagacaa cgccgatatg cgcctgaccg aagacggcta caaaatcggc
    14161 ttggtgggtg aagcgcaatg gcgcatgttc aacgaaaaac gcgaagccat cgaacgcgaa
    14221 atccaacgtt tgaaaacaac gtggtacacg ccgcaaaaac tcgccgaaga cgaacaaatc
    14281 cgcgtattcg ggcaaaagct cagccgcgaa gccaacctgc acgacctcct gcgccgcccg
    14341 aacctcgact acgccgcgct gatgacgctc gaaggcgcga tgccgtctga acgcctctcc
    14401 gccgaagtgg tcgaacaagt cgaaatccaa gtcaaatacc aaggctatat cgaccgccaa
    14461 aacgaagaaa tcgacagccg ccgcgacatc gaaaccttaa agctgcccga ggatatcgat
    14521 tacagcaaag ttaaaggttt gtcggcagaa gtgcagcaaa agctcaacca gcacaaaccc
    14581 gaaaccgtcg ggcaggcaag ccgcatttcc ggcgtaaccc ctgcggcagt ggcattgctg
    14641 atggtgcatt tgaagcgcgg gtttaaagac gcgaaataaa cacatcggcg cgatgccgtc
    14701 tgaaacaccg ttcagacggc atcgcgccat cccgacagga aacatcatgc acatactgac
    14761 cgccggcgtg gacgaggcag ggcgcgggcc tttagtcggc agcgtgtttg ccgccgccgt
    14821 catccttccg gaaatattcg acctgcccgg actgaccgac tccaaaaaac tcagcgagaa
    14881 aaaacgcgac gcgcttgccg aaatgataaa aaatcaggcg gttgcgtggc acgttgccgc
    14941 cgcctcgccc gaagaaatcg ccagcctcaa catcctgcac gccaccatgc tcgcgatgaa
    15001 acgcgccgtt gacggtttgg ctgtgcgtcc cgaaagaata ttcatcgacg gcaaccgcat
    15061 tcccgaacat ttgaacatcc ctgccgaagc cgtcgtcaaa ggcgacagca aaatcatcga
    15121 aatctccgcc gcatccgttt tggcaaagac cgcacgcgat gcggaaatgt acgcactggc
    15181 gcaacgccat ccccaatacg gtttcgacaa acacaaaggt tacggcacga agcagcatct
    15241 ggaagccctc aaacaatacg gcgtgctgcc cgaacatcgc cacgacttcg cccccgtcag
    15301 aaacctgctc gcgcagcaga cgctgtttta aaccggcaca aaaatgccgt ctgaacttca
    15361 gacggcattt ccagcatcgc aacttcaaag agaaagaacc cataccgcca ttcccgcgaa
    15421 aaacagaaaa tcaaaaaaga aacctaaaat ccgtcattcc cgcgcaggcg ggaatccaat
    15481 ccgtccggtt tccgtttttt ttgaatttca ggtaacttcc aaaccgccat tcccgcaaaa
    15541 gcgggaatct agaaactcaa agctgcaaga atttatcaaa aatgactgaa gctcaaaaaa
    15601 ccggattcct acgaaaacag gaatccggag tctcagggct ggcaaaaccg ttttacccga
    15661 taagtttccg taccgacaga cctagattcc cgccttcgcg ggaatgacga aattttaggc
    15721 ttctgttttg attttttgtt tttgcgggaa tgacgaaatt ttagattgca ggcatttatc
    15781 ggataaaaca gaaattaagc gtgacgaaaa tttatccgaa atcacagcaa cttttccgcg
    15841 tcattcccgc aaaagcggga atctagaaac tcaaagctgc aagaatttat caaaaatgac
    15901 tgaaactcaa aaaaccggat tcccgcgaaa acaggaatcc ggagtctcag ggttggaaaa
    15961 accgtttttc ccgataagtt tccgtaccga cagacctaga ttcccgcctg cacgggaatg
    16021 acgatatttc agttttgtgt ttttgatttt tttgtttttg cgggaatgac gatatagtgg
    16081 attaacaaaa atca
//
LOCUS       DAXHMX010000032        15376 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHMX010000032 DAXHMX010000000
VERSION     DAXHMX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.032"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="ACFM4S_000764"
     CDS             complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="ACFM4S_000764"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6050491.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAK
                     SRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1909..2346)
                     /locus_tag="ACFM4S_000765"
     CDS             complement(1909..2346)
                     /locus_tag="ACFM4S_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050492.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2440..4038)
                     /gene="aceF"
                     /locus_tag="ACFM4S_000766"
     CDS             complement(2440..4038)
                     /gene="aceF"
                     /locus_tag="ACFM4S_000766"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6050493.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGASAAEAAPAVAAEAQPAPAAAPV
                     AAGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGV
                     VKAVFLKVGDKVSEGSAIIEVETAGSVAAAPAQAAQAAAPAPTAAAAPAAAPAPSAPA
                     AAKIDEAAFAKAHAGPSARKLARELGVDLSQVKGTGLKGRIVGDDIKAFVKSVMQGGA
                     AKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTV
                     HEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLML
                     KNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACF
                     TISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVI
                     DGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4188..6851)
                     /gene="aceE"
                     /locus_tag="ACFM4S_000767"
     CDS             complement(4188..6851)
                     /gene="aceE"
                     /locus_tag="ACFM4S_000767"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6050494.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7545..9257
                     /locus_tag="ACFM4S_000768"
     CDS             7545..9257
                     /locus_tag="ACFM4S_000768"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6050495.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANVELAPTLPLKGERTAAQAELVKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQVMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            9320..9976
                     /locus_tag="ACFM4S_000769"
     CDS             9320..9976
                     /locus_tag="ACFM4S_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6050496.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9986..10441)
                     /gene="ruvX"
                     /locus_tag="ACFM4S_000770"
     CDS             complement(9986..10441)
                     /gene="ruvX"
                     /locus_tag="ACFM4S_000770"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6050497.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSHKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10434..10982)
                     /locus_tag="ACFM4S_000771"
     CDS             complement(10434..10982)
                     /locus_tag="ACFM4S_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6050498.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10984..11544)
                     /locus_tag="ACFM4S_000772"
     CDS             complement(10984..11544)
                     /locus_tag="ACFM4S_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6050499.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11872..13677
                     /locus_tag="ACFM4S_000773"
     CDS             11872..13677
                     /locus_tag="ACFM4S_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6050500.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSVRKGHEGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13797..15281
                     /locus_tag="ACFM4S_000774"
     CDS             13797..15281
                     /locus_tag="ACFM4S_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6050501.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELLKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3677 a         3780 c         4257 g         3662 t
ORIGIN      
        1 ccggaaaaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacgacc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggaggctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac gttggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttcg gtgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagtgg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc gggtctaaga
     1201 attgcccgtc gccttggata acgtccactt tacggctttt cgccatacct gccaaaccgc
     1261 ccgtgaggcg ggaaactacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg accgccaccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 caggtgcttc agctttggga gcggctgccg tgccttcagc ttcaacgacg acaatcaagc
     1681 cgccttcgga gattttgtcg ccgactttga ctttcacttc tttgaccacg cccgcaactt
     1741 cggcaggcac gtccatcgtc gctttgtcgg tttccagggt aatcagggta tcgtcaacgg
     1801 cgatggtgtc tcctacgttc acttcaactg cgataatatc tacattttcg tgtccgccaa
     1861 tgtcgggcac tttcaattca actaaagcca ttatattgac ctttcaactt agttaatttc
     1921 tatcaactct ttagcaataa tcacttcttc aggaatacag ccacaatctt cagctctgat
     1981 tgttacttcc ttctcatttg ggttttctgg atgttcaacc tcatattcaa aaatagaata
     2041 ttgaccaaac aaatccctat ttaaaacata tatatagcca ttttcgatgc cggaacttgt
     2101 tgcaaatttc ttggcaattt ccttgtctgt tgtcgtagat atataacatc cgtcatatag
     2161 acctgtttca atttgatggg cgtaaactgc attcttcaca cttggaccat gtgtagcttt
     2221 accatcatat ttaaacttac catcataacg aattgcaact tcagctttat tacctttagg
     2281 ttttaactgt ccattatttt gctcatcttg ttggcaacta atgcctctat ataagaaatt
     2341 tcccataagt tattaattta aaggttgaat ttaacaccat tactcatcaa tctcaagcaa
     2401 tccaaacatt atcagactgc ttgagaggga tgttttattt tataaggtaa tgcggcggaa
     2461 gtctttcaac agcttcgcca agaatacggt gaagcgcata ccggccgcac cgtcgatgac
     2521 gcggtggtcg aaggacaggc tcaacgggca catcaggcgc ggggcaaact ctttgccgtt
     2581 ccaaaccggt ttgatttggg atttgcacac gcccaagatg gcgacttcgg gagcgttcac
     2641 aatcggcgtg aagcctgtgc cgccgatgcc gcccaagctg gaaatggtaa agcatgcgcc
     2701 ttgcatttct tgtggtttga gcttgccttc gcgggctttt ttggacaatt ctgtcagctc
     2761 ttggctgatt tgtttcaagc ctttttgatc cacgtctttg atgacgggaa caaccaagcc
     2821 gttcggcgta tcggctgcga aaccgatgtt gaagtagttt ttcagcatca ggttgtcgcc
     2881 gtccagagaa gcgttgaatt cggggaaggc tttcagcgcg gaaacggagg ctttgatgat
     2941 gaacgccaga ggagacagtt tcacgccttc gcgttcccat tctttgttca gttgtttgcg
     3001 gaattcttcc agctcggtca tatctgcttc ttcgtgtacg gtaacgtggg gaatcaccac
     3061 ccagttgcga gacaggtttt gaccggagat tttcttaatg cgggacaatt ctttaacttc
     3121 gacattgccg aatttggaga agtccacttt aggccacggc agcaagtcca gaccgctgcc
     3181 caaagatgcg ccggctgcgg caggttttgc cgcgccaccc tgcattacgg atttcacaaa
     3241 ggctttgatg tcgtcgccca cgatacggcc tttcaagccg gtacctttga cttggctcaa
     3301 atccacgccc aattcgcgcg ccagtttgcg tgcggaaggg ccggcgtgtg ctttggcgaa
     3361 agcggcctcg tcgattttgg cagcggcagg tgcagaaggt gcaggcgcgg cggcgggtgc
     3421 ggcggcagca gtcggagccg gtgcggcagc ttgagcggct tgagcaggag ctgctgcgac
     3481 agagccggcg gtttccactt cgataatggc agagccttcg gacactttgt cgccgacttt
     3541 taagaatacg gctttaacga cgccggcagc ggtacaaggt acgtccattg tcgctttgtc
     3601 ggtttccaaa gtaatcagcg tgtcgtcttc ggcaacggtg tcgcccactt tgatttcaac
     3661 ggcgattaca tccacatcgg tatgaccgcc gatatcggga acggctactt gaacggttgc
     3721 accgcctgcg gcaacgggtg cggcggcagg tgcaggttgt gcttcagcgg caacagccgg
     3781 agcggcttcg gcggcggagg caccggtttc aacggtcaaa attacgccgc cttcggagat
     3841 tttgtcgccg actttgactt ttacttcttt cacgacaccg gcagcatcgg caggcacatc
     3901 catcgtggct ttgtcggttt ccagtgtaat cagggtgtcg tcaacggcga tggtgtcgcc
     3961 cgctttaact tctacggcga tgatgtcgac gttttcgtga ccgccgatat cggggacttt
     4021 gatttctacg atactcattt gagttccttt aatcttgttc agttggatgc cgtctgaaag
     4081 ctgcttcagg cggcatcggt acggtttgtc tgctcaaacc attcgggcat tcggttttca
     4141 gacggcctga tgtcggaatg gggcaaacag gccgtctgaa acatcaatca gcgtttccag
     4201 ctaggagctg aatcggcttt gatgccgtat ttctcaatgg cttgttgaac ggtttctttg
     4261 ctgactttgc cttgttccgc caatgcggcc aatgcggcca cggcaacgtt gtagcgatcc
     4321 acttcaaaga agcggcgcag gttggcgcgg ctgtcggagc ggccgaagcc gtcagtaccc
     4381 aagacgtggt agtcgttcgg gatgtaggcg cggatacggt cagcatagct gcggatatag
     4441 tcggtagcgg caataaccgg accgtcatga ccttgcaatt tggaagtaac gaaaggtact
     4501 ttttcggctt ccagcggatg caggcggttg aagcgttcta cctcgacagc gtcgcggtgc
     4561 agcaggttga aggatgggca agaccaaatg tctgcttcca cgccgaagtc ggctttcagc
     4621 agttcggcac cggcaatcac ttcttgcaaa atcgtaccgg aacccatcag ttggactttc
     4681 ttgtcgcctt tgccgccggc tttcagcagg tacatacctt tcaagatgtc ttgttccgca
     4741 ccttcgggca tatccggatg ggtgtagttc tcgttcatca gggtgatgta gtagaacacg
     4801 tcttcattat tggcatacat acggcgcaga ccgtcttgta cgatgacggc gacttcgtat
     4861 tggaaagtcg ggtcataaga tacgcagttc ggaatcaggt cggcctggat gtggctgtgg
     4921 ccgtcttcgt gttgcaggcc ttcgccgttc agcgtcgtac ggccggcagt accgcccagc
     4981 aggaagccgc gcgcgtgcat atcgcccgcc gcccaagcca agtcgccgat acgttggaaa
     5041 ccgaacatag aatagtaaat gtagaacgga atcatggcga agttgctgtt ggcgtagctg
     5101 gttgcagccg caatccagtc ggccatcgcg cccggttcgt taataccttc ttgcaagatt
     5161 tgaccgtcaa cggattcttt atagaacatc agttggtctt tgtcttgagg ggtatattgc
     5221 tgacctttcg gattccaaat accgtattgg cggaacatac cttccatgcc gaaagtacgg
     5281 ctttcgtcgg gaacgatagg tacgacgcgt ttgccgattt ttttgtcttt cagcaaagtg
     5341 gacaggatgc ggacgaatgc catcgtggtc gagaactcgc gttcaccgct ggatttgagt
     5401 tgtgcgtcga atgctgacag ctcgggcact tccaatactt cctgcgtcgg tttgcgttgc
     5461 ggcaggtagc cgcccaaagc atcgcggcgt gcgtgcaggt atttgtattc ttccgtatcg
     5521 ggggcaaaag tcaggtaagg cagatcgccg ctttcgattt gttcgtcggt aaccggaatg
     5581 tcaaagcggt cgcggaattg tttcagggac gctttgtcca tttttttggc ttggtgggca
     5641 acgttctgac cttcgccgga tgcgcccata ccgtaacctt taatggtttt cgccaagatg
     5701 acggtaggtt tgccgtcggc atggttcgct gcgcggtcgt aggcgttgta cactttttgc
     5761 gggtcgtggc cgccgcggtt caatgcccag agttgctcat cggtcatatc ggcaaccaat
     5821 gctttcagtt cgggcgtatt gaagaagtgt tcgcgcacat acgcgccgtc tttggatttg
     5881 taagtttggt agtcgccgtc caaacattct tccatacgtt ggcgcaggat accgtctttg
     5941 tctttcgcca agaggcggtc ccagcggcgg ccccaaatga ctttgacgac attccagccg
     6001 gcgccggcaa agttgccttc caattcttgg atgattttgc cgttgccgcg taccggaccg
     6061 tccaagcgtt gcagattgca gttgatgacg aaaatcaggt tgtccaagcc ttcgcgtgca
     6121 gccagtgcga ttgcaccttg agattcgggt tcgtccattt cgccgtcgcc gcagaaacac
     6181 catactttac ggcctttggt ttttgccaaa ccacgcgatt ccaagtattt caggaaacgc
     6241 gcctgataaa tcgccatgat gggccccaag cccatggata cggtcgggaa ctgccaaaag
     6301 tcgggcaaga ggtgggggtg cggataggaa ggcagaccgt gtccgtccac ttcttggcgg
     6361 aagttgttca gctggtcttc ggtcagacgg ccctcgacga atgcgcgtgc atagatgccc
     6421 ggggcgacgt gaccttggaa gaagaccaaa tcgccttctt cgccttcgcc tttggctttc
     6481 caaaagtggt tgaaaccgac ttcgtacatg gtggcggcag attggaaaga tgcgatgtgc
     6541 ccacccagtt ccaaatcttt cttgccggcg cgcaatacga tggcggcggc gttccagcgc
     6601 acgaatgcgc ggatgcggtg ttcgatgttt tggtcgcccg gaatgccttt ttcgttttca
     6661 accgaaacgg tattcaaata cggggtggtc gtgccgtgtg gcatacgtac gcccttgtcg
     6721 cggcagtatt tgaccaggtt ttccaagagg tattgcgcgc gttcgccgcc ttcatattcg
     6781 aggacggagc ttaacgcgtc cagccactct tgggtttcga tagggtcaac atcgtgtaat
     6841 tgggtggaca tagtatctat cctttatgtt gagtgtttaa tcggcaggga gggtgtagcc
     6901 aaccccttta ttttcgtttg tgaaaaataa taagcaaaaa cgaaaatttc taaatcaaac
     6961 cgaaaaaaat aatttaagtt taatgttttc aaaaaattat aaaagttttg gaaacaaggg
     7021 aaaacttgaa aaaaatgttc gatctgctcc acacgaaatc ctatcagcaa tcgggtctgc
     7081 tggcaaataa ccatgcctgt aaaagcaggt tttatactga agttggcgtt tggtaaggtc
     7141 aagtcttttt gatttcgaaa tattctgttt gattttggcg atattttttc ggaattgtgg
     7201 tatatcgaat cattatgtct ctgtcggcaa ttttcctatt gtagaaaatc tatacacaag
     7261 ctttgtggat aaccgtgttg ataagccacg gcttgtgtta aaaaagtctt acgcaacaaa
     7321 tgtacgctta ttttgattaa aaaataggca catttaaatc tataaaaatc atgtgcttat
     7381 gttcaatatg atgtttctgg tcatgttttt gccgtctgtt atgggatttg tcccgcgctt
     7441 ggggcagggg gtgaattttg gaggttggtg caaaacctgc ttttttcgcg ttacaatctg
     7501 cacccgattt acttttgaat ttgcctgttt ggaaaaatac ttttatgaaa gccagccaat
     7561 tctttatctc tactttaaaa gaagcccctg ccgaagccgc gcttgccagc cacaagctga
     7621 tgattcgcgc cggtctgatt aaagccaacg cgtccggcct ttatacttgg atgccgatgg
     7681 ggctgcgcgt gttacgcaaa gtcgaaaacg tcgtgcgcga ggaaatggcg cgcgcgggca
     7741 gcgtggagct gctgatgccg gtggtgcagc ctgccgagct gtggcaggaa tccggccgct
     7801 gggaatttta cggtaaagaa ctgctgcgcc tgaaagaccg ccacgaccgc gatttctgca
     7861 tgggaccgac ctgcgaggaa gtcatcgccg acatcgtgcg taaagagatc aacagctaca
     7921 aacaactgcc gaaaaacttt taccacatcc aaaccaaatt ccgtgacgaa gtgcgcccgc
     7981 gcttcggtgt gatgcgtgcg cgcgagtttg tcatgaaaga tgcttattcc ttccatgccg
     8041 actacgcctc gcttcagacg acctatgatg ccatgtatga cgcttactgc cgcatcttca
     8101 cccgtctggg cttggcattc cgccccgtcg ccgcagatac cggcagcatc ggcggtaccg
     8161 gctctcacga gtttcaagtg ttggcggaaa gcggcgaaga tgtgattgca tacagcgaca
     8221 cttccgatta cgccgccaat gtcgagctgg caccgacctt gccgcttaaa ggcgaacgca
     8281 ccgctgctca ggcagaactc gtcaaagtac atacaccaaa cgtcaaaacc attgagtctt
     8341 tggttgaatt cctgaatatt ccggttgaac aaaccctcaa atccatcgtg gttgaaggcg
     8401 aaaatgaagg cgaaatcgtt ttattgctgt tgcgcggcga ccatgagttc aacgacatca
     8461 aggcagaaaa actggcgggc gtgaaatcgc cattgacgat ggcaagttct gccgcgattg
     8521 tcgaacagtt cggcgcaaac ggcggctcgc tgggtccggt cggctttgca ggtaaagttt
     8581 atgccgattt cgccactgaa aaaggcgcgg actgggtcat cggtgcgaac gaagacggct
     8641 accactatac cggcttcaac ttcgggcgcg atgccgccga gcctgaattc gtcgatttgc
     8701 gtaatgtggt cgaaggcgac gaaagccccg acgggcaagg ccgtctgaaa ctggcgcgcg
     8761 gcatcgaagt cggacacgtt ttccaattgc gcgacaaata cacccaagcc atgaacgtaa
     8821 gcttccttga caacaacggc aaatcgcaag ttatggaaat gggctgctac ggcatcggca
     8881 tcacccgcgt cgttgccgcc gccatcgagc agaataacga cgaaaaaggc atcatctgga
     8941 ccaaagcaat ggcgccgttt gaagtcgtta tcgtgccgat gaactacaaa aaatcagaca
     9001 ccgtacgcga agccgccgac aaaatctatg ccgaattgct ggcggcaggc gcagacgtcc
     9061 tcctcgacga ccgcgacgaa cgcgcaggcg tgttgctgaa cgattccgag ctgttgggta
     9121 ttccgcaccg catcgtcatc ggcgaccgcg ctttgaaaga aggcaatgtc gaatacgccg
     9181 aacgccgcaa caacgaagcg caggcagttg caatcggaga aattgttgcg cgtgtaacag
     9241 cttcattaaa tgggtaagct tggtcaaatg ccgtctgaaa ataatttcag acggcatttt
     9301 tatttgggga gaaaatgaag tggcttcagt atttttagaa ggagaagcag tcgaggtcgg
     9361 caatattttc tgtatcggca ggaactatgc cgcacatatt gaagagttaa aaaacgaaat
     9421 accttccgaa cctgtggtgt ttatgaagcc gtcaggcagc attttgaata gcggcggtac
     9481 aatcttgctg cccgagttta gtagggatgt gcagtttgag tgcgaactcg tgctgctggt
     9541 cggtaaggat tcagacggca tgggagaggg tgaagatatt ttgggatgtg tggcggggta
     9601 cggcgtgggt ttggatttga ccgcgcgaga catccaatgc catctgaagg cgcaggggct
     9661 gccctggctg aaggcaaaag ggttcaggca ttccgcgtgc gtgtcggatt ttgcggcggc
     9721 agacagaatc ggcaatcccg agaaggtgtt gttttcatta aaacaaaacg gcgtattgaa
     9781 acagcgcggg gataccggtt tgatgattta cccgattcgg gaaattttac acaaactggc
     9841 ggcagattac ggtttgggga aaggggattt ggtgtttacc ggaacgcctt ccggcgtggg
     9901 cgcaatcggt gcgggggaca atttggcatt ggagctggac ggcttggtgc gcgcctcttt
     9961 cacggtaggg tgttaaccgc gccgcttaac cctcacgccc gttgaaacat tccgccggac
    10021 cgccctcgaa aaaaccgtgc aagatggctt gcgccgccac ttggtcgagc accgatttgc
    10081 gttttttgcc gaagacctgt gcttccgaaa gcaggctttc ggcatagacg gacgacagcc
    10141 gttcgtcaac ccaatagacg gggagattga acctgccgtt cagcctgcgt ccgaacttgt
    10201 gcgacaggtg cgtcatttca tgtttcgtgc cgtcggtatg cacgggcagt ccgacgacaa
    10261 aataacgcgg ctgccattct tgaaccagct tggcgattgc cgcgaacttt tcatcgttgc
    10321 tgccgccggt aacggtgctc aaaggatggg atagccctaa ttccgcgtct ccttgtgcca
    10381 cgccgatacg cgcttcgccg aagtcgaatg ccagtgccgt tccttttgga attttatgca
    10441 tggccggctc ccgaaaacag ggcgagcggg tcgatgccga gtttggcgaa tgcggcggcg
    10501 taacggtgtt cgtaggggat gtcgaacagg atgtgttcgt cggcgggaac agtcagccac
    10561 gcattgtcgg caagttcgcg ttcgagctgg ccttttcccc agcttgaata gcctatgctg
    10621 atcaaggctt tgtcaaccgc accttcgcgt gaaatatttt caatcacgtc tcgggaagaa
    10681 gttagcgcga tattgtctga aacgccgata ctgctttgcc agttgccgat cggggtatgc
    10741 acgacataac ctcgctcgac ttgcaccggc ccgcccatca tcacgctgtc gtgctgcatc
    10801 cgcatgggga tgtttttgcc ggtggcggaa aaaatcatgt ccatcgtaat cggagagggt
    10861 ttgttgatgg cgatgccgag tgcgccgtct tcatcgtgtt tgcagatata gacgaccgat
    10921 tgtgaaaaaa acgcgtcttc catatcgggc atggcgacga gaaagtggtt ggagaggttc
    10981 atatcagcgt ttgtcgggat tttctatcgg gttggaaatc atgcaggcgc cggcaagcag
    11041 gttgccggaa gtatcggctt gcaccccatc ggtttgcggt atgccgccgc cgaaacagga
    11101 aaccgcgctt aaggaatttt tcggcgagcc ttggatgatt ttatcgctgt acaccaaata
    11161 ggcgaaggtt ttgcgtttgg ggtcgtaata acggacaatc tgccggctct tgaacgcgaa
    11221 gctcgcaccg tgtttgaaaa cttctttcgg tttgcgcacg gcggtttcgt caaaagaaat
    11281 cgaagatgcc gtctgaacgc acgaaaccga tgcgtcggac gcgtcctctt ccaaattgac
    11341 catttccttc aagccgcctt tttttgcata cgaaatataa caggcaaccc cttgaacgtc
    11401 gggatcgtcg aatccttcca cttcgatacg gtcgtttttg cccagtatgt tgaaaacggt
    11461 acttgcccgt ccgattttat cggtttcgcc gctgccgcag gcagtcagca ggacggcggc
    11521 agacagcagt agcagtctgt tcatcaaaat ttccttatcg gttgaatccc cgttttccgt
    11581 gaaggcggca gggcttgctt tgtttggagg ggcgcacgtt cccaaaatcg ggcaacatac
    11641 ggtatgcggc ttaatgtgct accctgtgtg tcggaacaca atatccgttg cggcattgaa
    11701 aatatgatgc acaatataag gctgtttgag ccgattgcaa gtatccgcgc aaagaaggcg
    11761 caagattggg caaacggcat tttcagacgg cataactgac agtataatcc aaacgtccgg
    11821 ctgctttcgt gcagccttca gtattatcca actttttccg aaggtcgaac catgcgctac
    11881 aaaccccttc tgcttgccct gatgctcgtt ttttccacgc ccgccgttgc cgcccacgac
    11941 gcggcacaca accgttccgc cgaagtgaaa aaacagacga agaacaaaaa agaacagccc
    12001 gaagcggcgg aaggcaaaaa agaaaaaggc aaaaatggcg cagtgaaaga taaaaaaaca
    12061 ggcggcaaag aggcggcaaa agagggcaaa gagtccaaaa aaaccgccaa aaaccgcaaa
    12121 gaagcagaga aggaggcgac atccaggcag tctgtgcgca aaggacacga aggggataag
    12181 aaatcgaagg cggaacacaa aaaggcacat ggcaagcccg tgtccggatc caaagaaaaa
    12241 aacgcaaaaa cacagcctga aaacaagcaa ggcaaaaaag aggcaaaagg acagggcaat
    12301 ccgcgcaagg gcggcaaggc ggaaaaagac actgtttctg caaataaaaa agtccgttcc
    12361 gacaagaacg gcaaagcagt gaaacaggac aaaaaataca gggaagagaa aaatgccaaa
    12421 accgattccg acgaattgaa agccgccgtt gccgctgcca ccaatgatgt cgaaaacaaa
    12481 aaagccctgc tcaaacaaag cgaaggaatg ctgcttcatg tcagcaattc cctcaaacag
    12541 cttcaggaag agcgtatccg ccaagaacgt atccgccaag agcgtatccg tcaggcgcgc
    12601 ggcaaccttg cttccgtcaa ccgcaaacag cgcgaggctt gggacaagtt ccaaaaactc
    12661 aataccgagc tgaaccgttt gaaaacggaa gtcgccgcta cgaaagcgca gatttcccgt
    12721 ttcgtatcgg ggaactataa aaacagccag ccgaatgcgg ttgccctgtt cctgaaaaac
    12781 gccgaaccgg gtcagaaaaa ccgctttttg cgttatacgc gttatgtaaa cgcctccaat
    12841 cgggaagttg tcaaggattt ggaaaaacag cagaaggctt tggcggtaca agagcagaaa
    12901 atcaacaatg agcttgcccg tttgaagaaa attcaggcaa acgtgcaatc cctgctgaaa
    12961 aaacagggtg taaccgatgc ggcggaacag acggaaagcc gcagacagaa tgccaaaatc
    13021 gccaaagatg cccgaaaact gctggaacag aaagggaacg agcagcagct gaacaagctc
    13081 ttgagcaatt tggagaagaa aaaggccgaa caccgcattc aggatgcgga agcaaaaaga
    13141 aaattggctg aagccagact ggcggcagcc gaaaaagcca gaaaagaagc ggcgcagcag
    13201 aaggctgaag cacgacgtgc ggaaatgtcc aacctgaccg ccgaagacag gaacatccaa
    13261 gcgccttcgg ttatgggtat cggcagtgcc gacggtttca gccgcatgca gggacgtttg
    13321 aaaaaaccgg ttgacggtgt gccgaccgga cttttcgggc agaaccggag cggcggcgat
    13381 gtttggaaag gcgtgttcta ttccactgca ccggcaacgg ttgaaagcat tgcgccggga
    13441 acggtaagct atgcggacga gttggacggc tacggcaaag tggtcgtggt cgatcacggc
    13501 gagaactaca tcagcatcta tgccggtttg agcgaaattt ccgtcggcaa gggttatatg
    13561 gtcgcggcag gaagcaaaat cggctcgagc gggtcgctgc cggacgggga agaggggctt
    13621 tacctgcaaa tacgttatca aggtcaggta ttgaaccctt cgagctggat acgttgatcg
    13681 gattgccgaa acggtaggct ttatgcaaat cgtgccgata tggtgcgctg ttccgtgaag
    13741 tttgccgtac ctgtattttc cggtttttgg aaatcaagag agaaagttaa agaagaatgt
    13801 cgaaacctgt ttttaagaaa atcgcacttt atactttggg tgcaatcagc ggcgtggccg
    13861 tcagtctggc ggtgcagggt tttgccgccg agaaggacag gcgggataac gaagtcctgc
    13921 cggtgcaatc catccgcaca atggcggagg tttacggtca aatcaaggca aactactatc
    13981 aggacaaacc cgatgccgat ttgtttgaag gtgcgatgaa gggtatggtg gccggtttgg
    14041 atccgcattc cgaatatatg gataaaaaag gttatgccga gataaaggag tccaccagcg
    14101 gcgaatttgg cggcttgggg atggaaatcg ggcaggaaga cggatttgtc aaagtggttt
    14161 cgccgattga ggacacgcct gcggaacggg cgggggtgaa aagcggcgat ttcattgtga
    14221 aaatcgataa tgtttcgaca cgcggcatga cggtcagcga agcggtgaag aaaatgcggg
    14281 gcaagccggg tacgaagatt actttgacgc tgtcgcgcaa aaatgccgac aagccgatag
    14341 tcgtcaacct gacccgtgcc attattaaag tgaaaagcgt ccgccatcac ctgatcgaac
    14401 ccgattacgg ctatatccgc gtgtcgcagt tccaagagcg gacggtcgaa agcgtcaata
    14461 ccgccgcaaa agagctgcta aaggaaaata aaggaaaacc gctcaagggg ctggtgttgg
    14521 atttgcgcga cgaccccggc gggcttttga ctggcgcggt cggcgtgtcg gcggcatttc
    14581 tgccgtctga agcagtcgtc gtcagcacca agggacgcga cggcaaagac cgcatggtac
    14641 tgaaagccat tcctgaagat tatgtgtacg ggatgggcgg cgattcgttg gcgggcattc
    14701 ctgccgagtt gaaaaccata ccgatgacgg tattggtcaa ttccggttcg gcttccgcgt
    14761 cggagattgt cgcaggtgca ttgcaggatc ataaacgcgc ggtcatcgtc ggtacgcaga
    14821 gcttcggcaa aggttcggtt cagactttga ttcctttgtc caacggcagc gcggtcaagc
    14881 tgacaacggc actgtattat acgccgaacg accgttctat tcaggcgcag gggattgttc
    14941 ccgatgtcga agtaaaagat aaggaacgca tttttgaaag ccgcgaggcg gatttggtcg
    15001 gacacatcgg caatcctttg ggcggcgagg atgtgaacag tgaaaccctt gccgtgccgc
    15061 ttgaaaaaga tgcggataag cccgctgtaa aagaaaaagg taaaaagaaa aaggacgagg
    15121 atttgtcttc aaggcggatt cccaaccctg ccaaagacga ccagttgcgg aaagctttgg
    15181 atttagtcaa gtcgcccgag cagtggcaga agtctttggg gctggcggcg aaaaagccgg
    15241 tttcaaataa agataagaaa gataaaaaag ataagaagta ggttttgacg atgccgtctg
    15301 aaaggatttc agacggcata ttatgtttga aaggacaggt tatgagcgat tttttaccgg
    15361 aatttgaacc gtgcgg
//
LOCUS       DAXHMX010000033        15350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHMX010000033 DAXHMX010000000
VERSION     DAXHMX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.033"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..238)
                     /locus_tag="ACFM4S_000775"
                     /pseudo
     CDS             complement(1..238)
                     /locus_tag="ACFM4S_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259517.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(419..2890)
                     /locus_tag="ACFM4S_000776"
     CDS             complement(419..2890)
                     /locus_tag="ACFM4S_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6050502.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIACRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRVSSGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LVVLGNAMRLHKQVDMPSEKMPSEQ"
     gene            complement(2887..4065)
                     /gene="hflX"
                     /locus_tag="ACFM4S_000777"
     CDS             complement(2887..4065)
                     /gene="hflX"
                     /locus_tag="ACFM4S_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6050503.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(4070..5350)
                     /locus_tag="ACFM4S_000778"
     CDS             complement(4070..5350)
                     /locus_tag="ACFM4S_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6050504.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            5352..5480
                     /locus_tag="ACFM4S_000779"
     CDS             5352..5480
                     /locus_tag="ACFM4S_000779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050505.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(5503..6228)
                     /locus_tag="ACFM4S_000780"
     CDS             complement(5503..6228)
                     /locus_tag="ACFM4S_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6050506.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(6301..6978)
                     /gene="radC"
                     /locus_tag="ACFM4S_000781"
     CDS             complement(6301..6978)
                     /gene="radC"
                     /locus_tag="ACFM4S_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6050507.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQSVCSF
                     RQLGLMP"
     gene            7005..7127
                     /locus_tag="ACFM4S_000782"
     CDS             7005..7127
                     /locus_tag="ACFM4S_000782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050508.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(7101..8450)
                     /gene="gshA"
                     /locus_tag="ACFM4S_000783"
     CDS             complement(7101..8450)
                     /gene="gshA"
                     /locus_tag="ACFM4S_000783"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6050509.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIGIDEWQINPYFEKIGGLDFQEREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            8710..10119
                     /gene="leuC"
                     /locus_tag="ACFM4S_000784"
     CDS             8710..10119
                     /gene="leuC"
                     /locus_tag="ACFM4S_000784"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6050510.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMESR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            10216..10470
                     /locus_tag="ACFM4S_000785"
     CDS             10216..10470
                     /locus_tag="ACFM4S_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050511.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            10532..11173
                     /gene="leuD"
                     /locus_tag="ACFM4S_000786"
     CDS             10532..11173
                     /gene="leuD"
                     /locus_tag="ACFM4S_000786"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6050512.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAGQTLT
                     TPGGETLAFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            11342..12613
                     /locus_tag="ACFM4S_000787"
     CDS             11342..12613
                     /locus_tag="ACFM4S_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6050513.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            12629..13360
                     /locus_tag="ACFM4S_000788"
     CDS             12629..13360
                     /locus_tag="ACFM4S_000788"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC6050514.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNASPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            13394..14464
                     /gene="leuB"
                     /locus_tag="ACFM4S_000789"
     CDS             13394..14464
                     /gene="leuB"
                     /locus_tag="ACFM4S_000789"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6050515.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            14627..15190
                     /locus_tag="ACFM4S_000790"
     CDS             14627..15190
                     /locus_tag="ACFM4S_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6050516.1"
                     /translation="MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPIANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMEKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         3732 a         4120 c         3898 g         3600 t
ORIGIN      
        1 tttctttgat atatcgttaa tatctcccta gtttttctta aaaaaatata atcatatata
       61 tactacacaa cattggtaat aaataaatta aaagcgaaag gtaattaaat tgataattgt
      121 aaattatata ataaacaaca tacgaagaaa aaattattat agatgattta ctatacgaat
      181 aaaaaaatat cctacctagc ccgaaccttc ttataatttg tactatgtga ttatccattt
      241 caatatccat ttaagctttc aattaatttt ctatattttt caatggcatg tatcatgcta
      301 tgcttgggaa atatcatctt ggttgatgat caagccatgt atgcaaagat tggaatatgg
      361 tatttgtcgt gctaataatg cccccgttac tgttatacgt ctaaagcaac gctgtccgtc
      421 attgttcgga cggcattttt tcagacggca tgtccacctg cttgtgaagc cgcatagcat
      481 tgcccaatac gaccagcgaa ctgaagctca tacccagtgc ggcaatccac ggttggacat
      541 agcccaaaac ggcaagcggt acggcaatga tattgtacgc gcccgcccat atcaggtttt
      601 gccggataat atggcgggtg cgccgcgcct gatcgagcag gtgggcgacg gtacgcaaat
      661 cttcgttcaa taacacaatg tccgcgccgt ccctcgcaat atccgtcccg cccgctgcgg
      721 cggcggatac gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg cctatcatca
      781 gcactttttt cccttctttt tgcaaggctt tgacgtattc cagtttgtcc tcgggcatgg
      841 cttgggcgcg gtagtgcgcg acacccaggg cgcgtgcggt ttcggcaacg gcggtttcgc
      901 ggtcgccgct gaggatgtgc agggtcaggt ttttgcctgc caactgccgc accgcctccg
      961 ccgcgctgtc tttcaagggg tcggtcaggt agaacacggc ttggaaaccg ctttgactgc
     1021 cgaggtaaac cgcgctgccg ccgccttctg tttgcggttc tttacctgaa atttcggcga
     1081 cataggatgc cctgcccaat gcccaaacct gtgtttcgcc gttgacggtc agttgcgcgc
     1141 ccacgccttc gccgatgcgg ttgaggcgtt gtttaatagc gatgtcgggg acgctgccgc
     1201 ttgaaacacg gcagttgagg atggcgcggg caaggggatg ttcggactgt tgttctaaag
     1261 cctgcgccac cgcgagaaca aaggcttcgt ctgtgcctct caacaatgag atacggcgga
     1321 cggcgggttt gccttgggtc agcgtgccgg ttttgtcgaa gatgatgtcg gtggtttggg
     1381 cgagggtttc gattgcctgc tttccgccga ttaaaatgcc ttcgcgcgcc agcgtaccgg
     1441 tagaagctgc cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag gtaatgacca
     1501 gcagggcgac ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc cagccgatga
     1561 agacggggac ggcaagcagg agttcgccga atatgaaaga cgaggcgtat tgttccgcca
     1621 actcggcagt gcgcggtttt tgcgctaagg cgcggtcgag caggcggacg atgtgcgaca
     1681 gtcgcgtgcc gccgccggtg cggtcggtgc gtataatcag ggggctttgc gtgttgagtg
     1741 tgccggcggt tactttttca gacggcattt tggcgacggg caggctctcg ccggtcagca
     1801 tagattcgtt gacggcactg cttccttcca gcaccgtgcc gtcaacgggg atggtttcgc
     1861 ccggtttgac cagcacgata tcgcccgctt tcaatttgac gacagctgcc tcgcaggttt
     1921 cctgcgtatc ggggtaatcg ggcatatgat ggcaaaacgc aggaatcagc ttcaccagcc
     1981 tctcggcggc atcgcctgcc ttacggcagg caatgtgttc cataaagcgt ccgcccagca
     2041 ggaaaaacag cagcatcgcg atggattcga aatacatccc ctgccccgca tttgtcgcaa
     2101 ggctgtaaac gccggcgata aaggtcataa tgatggcgac ggtaatcggc gtgtccatac
     2161 cgacccgacg gtttttcagg tcgcgcaacg cgccttgata aaacgggacg gcgcaataaa
     2221 atacgacggg cagcaccatt aaaaagccgc cccaatggag gatttgcagg aaatcgggtt
     2281 cgatatcgcc gccgtaaagg taggtcggca gcgcgaacat catcgtctgc atcatcccca
     2341 gcccggcaac ggcgaggcgg acgatgtatt gtttgcgttc tttttggttg gcggcttcga
     2401 ttttttgcgc gtcatagggt gcggcggtgt agcctatctg ccggattttc aacagaatat
     2461 cggaaaggcg gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg ctgtaattga
     2521 ggtcgatgcg gacgatgccg tctgtacgca aaagctgctg ttcgatcagc cagacgcagg
     2581 cggcgcaggt gatgccgccg agcattaaaa ccgcctcgcg cgtgccgccg tgggtttcca
     2641 caaagtcgga ctggacttcg ggcaggtcgt acaggcggat ttggtcgagg atttcttggg
     2701 gcggcagctc ggttttttgc gcgtcggcgg tgcgttgttt gtaataactg cccaagcccg
     2761 cgtcaataat gctttgtgcg accgcctgac agccggcgca gcaggtttct cggtcttcgc
     2821 cttcgtagcg gacggtcaga tgcaggtttt cgggaacatc cagcccgcag tggaaacagg
     2881 tttttttcat ggcatttcgg tttcgtctgt gtttggtgcg gcggcacaag actcggcaat
     2941 ggcttcgcgc agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga cggcggcaat
     3001 ttttcccgca gcgtcgcgcc atatgcccgt gttttgttct tcagacggca gcaggtcggt
     3061 tttgttgtac accttgatgc acggaatatc gccggcatgg atttcttgca gtacgttttc
     3121 cacgtcttca atctgctgtc cgctgttcgg ggcggcggca tcgacgacgt gcagcagcac
     3181 atcggcttgc gcggtttctt ccagcgtggc ggaaaatgcc gaaatcagtt tgtgcggcaa
     3241 atcactgacg aatccgacgg tatcggtcag gataatgctg cattcgggac tgatgtacag
     3301 ccgccgcgcc gtggtgtcga gtgtggcgaa aagctggtct ttcgcatata tgcccgactt
     3361 ggtcagccgg ttgaacaggc tggatttgcc gacattggta tagccgacca gcgcaaacgt
     3421 tttgattgtg cccgattcgc ggggcttgcg gcgcagggcg cgctgttttt tgaggttgac
     3481 aagctgtttt ttcaaggcat tgatccgatg ggcgatcaat cggcggtcgg tttccagttt
     3541 ggtttcgccg gggcctttca tgccgatacc gccgcgctgg ctctgcagat ggccgtaacc
     3601 gcgtatcaag cgtcccgcca aatggctcaa ttgcgccaac tcgacttgca gcctgccttc
     3661 ctgcgtgcgg gcgcggcggg cgaaaatcgc cagaatcagc cctaccctgt ccaatacgcg
     3721 gcatttcagt tctttttcaa ggttgcgttc ctgcgtgggc gtgagttcgt ggttgaatac
     3781 gaccaaatcg atgccgtctg cggcaactgc ttctgacagc tccgccgcct tgcccgtgcc
     3841 gacaaacagc gcggtgtgcg gacggtcgcg tttggcagtc tccacgcgca cggaatcgcc
     3901 gcccgccgct ttgaccagct cgacagcttc cgccaatgcc gtctgaaaac cgttcagacg
     3961 ggtggcactg ctgcccgtgc catctttgtc caacattacg cccaccagca tgacgcgttc
     4021 gggttgcgct tgggtactgc cgttgcggcc tgttctgcct gtcatgtatt caatcctgta
     4081 aataaagggt tacgcctcgt tccgtttctc cttccggtgc aagcgtgatg cggctgcccg
     4141 ccattccgtc aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg gacaggcggc
     4201 tgccgggtcg ctgccggtag gtataacggg tacacgcttc caaacaccag cgtccgcgcc
     4261 agtggatgaa atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg tttacctcaa
     4321 aataagggta atcgggtttt cgggttctaa actgccattt cgcctgcgcg cgccgtccgt
     4381 tgcggacata gtcgaatacc gccgtataaa gcgtaccgta ttccaaaggt ttgagcggaa
     4441 aaagcgcgaa ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg gttaaaaccc
     4501 tgacggggcg gatttcgttt ttaccctgat acagcttgaa acttttcatc gtaattttgc
     4561 ctgccgcctc ggaaaaatca atgctggcag gattgcccgt gatttcatat tccggcacgg
     4621 ggtctggacg ctcgccgtgg aaataaggca gtgcgccgct gccgacggga taggctgtat
     4681 agagcagctc ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg ttatggcagg
     4741 cgttgcggta atatttccgt cctgcttccg gctgatttct gccttgggcg caatgccgct
     4801 caaacctgcc gttgccctga ttgaatacga gaacggtttt accgttttcg cgcacaaatg
     4861 ccgctcctgc ctcatccgta tggcggtcaa gtagggaaag gcggtggtag attgcgctca
     4921 ttaatccgtc cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat tcggcggctt
     4981 cctcttccgt gctgatgttt tcatgcacgc cgttgtagag atacccggca aggcgtgtgc
     5041 gttcggtcag cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg ccgtgtccgt
     5101 cttcgggatt gagcgtgaga tagcgtgcgt gcctgcgggc ggaattttcc aaaaccggcg
     5161 cgtgtgccag cttgtgcaaa ccgatttggg cgcggatgcg gttgaggtag tgcagcgcat
     5221 cgccgccgtc cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg ggcagggatt
     5281 ggttttgggt atgatagaaa acgcctgccg ccgaacccaa caggagcagc cgaataaaaa
     5341 gggatttcat aatgccgtcc tcccgacgcg gcaacggggt gttttatcaa aacggcaaac
     5401 ttgccaatgc ggtttccgat tgcagattgc caaaccggca aacctttccc cgtgccgatg
     5461 ccgaacccga tgccgtctga accttcagac ggcatcgggt gtttatttgt ccacccggat
     5521 gggggcaggt tcgtattgtg tcgattcgtc gccgtaatac agcacgccga gtttgatggg
     5581 gattctgccc tgtgatttgc ggtgggcatt ggaatccctc agggaatagg cacaaccgca
     5641 atattcctgc tggtagaagt tttcacgttt gctgatttca atcatgcgcg cgctgccgcc
     5701 gcctttgcgc cagttgaaat cccaatacac cacatcatcg taaggcgcgg cggcgcggtg
     5761 tccgcagtcg ttgatttgcg ccatattttt ccagcgtgaa atgcccagcg aactggtaaa
     5821 gacggggaac ccgtgttcat gcgcgtattg cgccgccttt tcaaaacgca tatcgaaaca
     5881 catggtgcag cggatgccgc gttccggctc aaactccatg cctttggctt tggcaaacca
     5941 ttctttgcgg tcgttttcgt agtcgtcgtc tttatcgatg aaaggaatgc cgaacttttc
     6001 cgcaaagcgc acgttttcct cttttcggag catatactct ttgtgcggat ggatattggg
     6061 attgtaaaaa taaatggtgt agtcgatgcc gctggccagc atggcttcca tcacttcgcc
     6121 gctgcacggg gcgcagcagg aatgcagcaa gactttttta tgtccaccgg gcgggacgag
     6181 tatagggcgg tcaatgtcgg taacggtagg tttgttttgt gtttccatta acttaacaat
     6241 atctgttgaa tacggttgaa gcggctattt tatcagagcc gccgcatgta aaacagagtg
     6301 tcagggcatc aacccgagct gtctgaacga acagacgctt tgcgaggtaa cgataaaatg
     6361 gtcgagcagc gacacatcga ccagcgacat tgcctgtgcc agccgccttg tgaacatgat
     6421 gtcttcctgc gaaggttcgg gcgagccgcc cggatggttg tgcgcaataa tcaggctgtc
     6481 ggcatattcg tccaatgcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac
     6541 cgtaccgcgc gacagctctc tgaccgcaat cagttggttt tggcggttca gcagcagcgc
     6601 gacgctgact tcgacttttt cctgccccaa atgaaagcgt aaataatcgg ccaccgtatc
     6661 cggatcggac aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc gcccgatttc
     6721 cctgaccacg gcaaactgtg tgaaacttgc cgtccccatc cctttgtatg ccgacagttt
     6781 gccgacctcc gcgctcatca gcctccccaa actgccgaac tcctgcagca aataacgcgc
     6841 caaatcgacc gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca aaagttcggc
     6901 atcactcaac gccgccgccc cgcgttccaa cagcttttcc ctgggtcttt cgccttccgg
     6961 ccattgcttg atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacag
     7021 atgttcccac acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc
     7081 atctgtattt caaaacagtc ttacgccgtt tcttccaact cgattgaagc cgccagaagc
     7141 gacaggcgtg ccattacgcc gtaaacgtag aggcggttgt gttcgttgtc cacgtcgcag
     7201 tctttttcag agcgcggcat acccagcgag tcccattgtt cgaatacgcg cttgcaggct
     7261 tcgggcgctt cttgggaatt atcgccgtta ccggtaggaa tgctgttaga cagcggaaca
     7321 aacaccatac cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa
     7381 aagccgccga tgacgaagcg gtccatcata tacacgacgg gttcgcacac cgcgccgttt
     7441 aaggtttcat aagtataaat cccttcttgg acaatcactt cgctgacttc caagccttct
     7501 ttgactttcg ccattttatt gcggttttta cggttcaatc cgcgcacttc gtcggcggat
     7561 ttgacgctca tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt
     7621 ttgtcggtaa tgcccgattc gtcgtatttg gcttgaattt tcgccagcac acgttctacc
     7681 gcttctgcca acgcatcttc accttcccgc tcttggaagt ccaaaccgcc gattttttca
     7741 aaataagggt taatctgcca ttcgtcgatg ccgattaact tggcaaattc ggcggcaact
     7801 tggttgtacg cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcaacggc
     7861 ggcaaaacgg tttggctgat gcctttgagg atgtcgggaa tgccggcgga caaatcgttg
     7921 ttcaacaaaa ccacgcaagg cgagaaaccg tctgcaagat gtacgcggtc gcgggtacgc
     7981 aataaaggtt ccaacaggat tttgtcgccc aatgcggttt caaattcggt cggttcggtt
     8041 acttccggat tcaggctgcc caagcgcact tcataccctg ccgaacacaa aatctcgccg
     8101 agggcgtaaa cgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg aataatcaat
     8161 acggatttcg ccgtttcaca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc
     8221 gggataaagt tgggattcag attattgaag ccgccgggga acaaattcat atcgatagac
     8281 gaaattttgt aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg gcggtgcgcg
     8341 ttccactgcg tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca
     8401 aacgcctgaa gttgcgccga atgttcgggc gacatcaccg gtaatttcat ctttcgtctg
     8461 cctctgtgtg ttttttgaca agatgcagat gataatgcct ttgaaagaat gacgacaagt
     8521 attctttcgt atcgaatcat gctttagata taactgttta ctgaaatgga caatatgtct
     8581 aattttttcg ttaaaattgt ttgcaaaata acaccggctc aaaaattaga caaaatttgt
     8641 tgcgcggtat aaagaatacg tctaaaatcc gcccaatccg cattaccatt tatccaaaag
     8701 aacaacatca tgacagcaca aaccctctac gacaaacttt ggaacagcca cgtcgtccgc
     8761 gaagaagaag acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc
     8821 agccctcagg catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagc
     8881 gtcgtctcca ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac
     8941 cctatttcca agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc
     9001 gcctattttc cgtttatgga caaaggtcag ggcatcgtac acgttatggg ccccgaacaa
     9061 ggcgcgaccc tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc cacccacggc
     9121 gcattcggcg cactggcgca cggcatcggc acttccgaag tcgagcacac gatggcgacc
     9181 cagtgcatta ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg caaattaaaa
     9241 gcgggcgtta ccgccaaaga cgtggcgctc tacatcatcg ggcaaatcgg cacggcaggc
     9301 ggtacaggct acgccatcga gtttggcggc gaagccatcc gcagcctttc tatggaaagc
     9361 cgcatgactt tatgcaatat ggcgattgag gcaggcgcgc gctcaggcat ggttgccgtc
     9421 gaccaaacca ccatcgacta cgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg
     9481 gacaaagccg tcgagtactg gcgtacgctg gtgtctgacg aaggtgcggt attcgacaaa
     9541 gaataccgtt tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa
     9601 atggttttag acatcagcag caaagtgccg aatcctgccg aagaaaccga tccggtcaaa
     9661 cgcagcggta tggaacgcgc ccttgaatac atgggcttgg aagccggtac gccattaaac
     9721 gaaatccccg tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg
     9781 cgcgaagccg ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgtta
     9841 atcgtccccg gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt
     9901 ttcatcgaag ccggttttga atggcgcgaa ccgggctgtt cgatgtgtct cgccatgaac
     9961 gccgaccgcc tgaccccggg gcaacgctgc gcctccacct ccaaccgtaa ctttgaaggc
    10021 cgtcaaggca acggcggacg tacccacctc gtcagccccg ctatggcagc agcagccgcc
    10081 gttaccggcc gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa
    10141 gaatttgacg cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt aatattcaaa
    10201 ccaaggagtt taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc
    10261 gcctgcgccg gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca
    10321 caggccgccg ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac
    10381 aaaaccaaag aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc
    10441 ggcggaaaaa tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgcttc
    10501 agacggcatc gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa ttaccgccat
    10561 cgtcgccccg ctcgaccgca gcaacgtcga taccgacgcc atcatcccca aacaattttt
    10621 aaaatccatc aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga
    10681 ccacggcgaa ccaggcatgg acaacagcaa acgcccgttg aaccctgatt tctcgctgaa
    10741 ccagccgcgt taccaaggcg cgcaaatcct gttgacgcgt aaaaacttcg gttgcggctc
    10801 ttcacgcgaa cacgcccctt gggcattgga cgactacggc ttccgcgccg tcatcgcccc
    10861 cagcttcgcc gacatcttct ttaacaactg ctacaaaaac ggccttttgc ctatcgtgtt
    10921 gaccgaagaa caggttgacc ggcttttcaa agaagttgaa gccaacgaag gctaccggct
    10981 ctccatcgac cttgccgggc aaaccctgac cacccccggc ggcgaaacat tagcattcga
    11041 cattaccgaa caccgcaaac actgcctctt aaacggcttg gacgaaatcg gactgaccct
    11101 gcaacacgcc gatgaaattc acgcctttga agaaaaacgc cgccaaagcc agccttggct
    11161 gtttaacggt taaacctatg ccgtctgaag accgttcaga cggcattcct gataacatat
    11221 aattgctgca actaatgtaa acagaggggc tataaattgc tttcatcttt caagcgggct
    11281 tagcaacgct ctaaattaat gcaatacagc tctatttcaa ataaattttt agatttttaa
    11341 tatgcaacag attaaattta ttgacttatt ttccgggatg agcggtatca ggaaaggatt
    11401 tgaacaagcc tgtcggaaac agtcggttgc ttgcgaatgt gtttttacct cagaaatcaa
    11461 accggcagct ttggaagtat tgaagcaaaa ctaccctgat gaagtgccgt atggagatat
    11521 aacgaaaatt gaaacgggag atattcccga ttttgacatc ttgttggcag gcttcccttg
    11581 ccaggctttt tctttcgccg gaaaaagatt gggctttgaa gatacgcggg gaacgctttt
    11641 ctttgatgtg gcaaggattt taaaggcaaa aaaaccaaaa ggttttattt tggaaaatgt
    11701 ggaaggattg gtgacgcacg atagaaaaga tccgacacaa aaaataggac gcaccctgac
    11761 cgttattttg gaaaccttgg aaacattggg ctactatgtt tcttggaaag ttttaaatgc
    11821 aaaagatttc ggcattcccc aaaacaggaa gcgcatttat ctgacaggca gtttgaaatc
    11881 caaacccgac ttatcttttg aaacaaccct aagtccgaaa ttaaaaaata ttttggaatc
    11941 gggactgcct accgaaagca gtcctttcat caaaaaattg ctaaagaaat ttcccccgtc
    12001 cgaactgtac ggaaaatcag taaaggacaa acggggaggg aaaaacaata tccacagttg
    12061 ggatattgaa ttaaaaggca cagtaaccga agaagagaag caattattaa atatgcttct
    12121 aaaagaacgg aggaagaaaa aatgggcttc agaaatcggc atcgattgga tggatgggat
    12181 gcctttgaca aaagcgcaaa tttcaacttt ctataaacac cccaatcttc aaaatatttt
    12241 ggacagcctg acagataaag gctatttggt tttggagcat ccgaaacaaa aaattggcgg
    12301 gcgaagaatc aaagatgaat ccctgcccaa aggctacaat attgtttcag gaaaaaaatc
    12361 atttgaaatc aataaaatat tagacccaaa tgatgttgcg ccaaccttag ttgcaatgga
    12421 tatggagcac cttttcgtcg ttgacaacgg cggtttgcgc acacttaccg gaaaagaagg
    12481 tttacgctta ttcggctatc cggacgatta ttcgtttgat attcctaaaa aagacaaata
    12541 cgatttattg ggcaataccg ttgccgttcc tgtgattaag gcggtatctg aaaggcttct
    12601 gcatacttta taaaccgata gggataaaat ggtaaaactt actgcacaac aaatatttga
    12661 taagttgctg aatgaggaaa aaatcttatc ggtcaatggt caaattagat ttttcttggg
    12721 cgatgtggat attatcgtca aacaaaaaga tgtcgtcggc aacatcattc aggaatggct
    12781 cggcggatgg ttgagaaaaa gggaaattga atttgatgtt tcaaccaata cccaaatgcc
    12841 ccccgatttc tttttaaata aaaaagaccg cagtagagaa ttgttggaag taaaggcgtt
    12901 caaccgaaat gccagtccgg gttttgatat tgcagatttt aaaatgtatt ctgatgaaat
    12961 cattcataaa ccctatatgc tggatgtaga ttatttaata ttcggttacg atatggacga
    13021 caacggcaat gtaaccatca aggatttatg gcttaaaaaa gtatggcaaa ttacgagaag
    13081 catggatggg tgggcaatta atcttcaagt caaaaaaggc gtggtgcata aaatccgccc
    13141 gggtgtttgg tacagcataa ataaaaagaa tatgcctatg tttgaatgct tggaagattt
    13201 tgtttccgca attgaagaaa ccgtttatca aaacccggca acacggcata acgcatcctt
    13261 atggaaaaag aaatttgagg aagcctataa aaaacactac aaccgatcga tttccatacc
    13321 tcgttggcac gaaattgcac acaaatacaa aaagaaataa cccttcaacc atcaacccat
    13381 aaggaaactt gaaatgacca aacatatcgc catcctccga ggtgacggca tcggtcccga
    13441 aatcgtcgcc gaaaccgtcc gcgtactcga caaactcatc gcccaaggtt tggatgccgg
    13501 ctacgaatac gccccattgg gcggcgaagc ctatgacgaa tacggtcatc cttatcccga
    13561 atttacgcaa aacctctgcc gcaaagccga tgccgtcctg cttggtgcag tcggttctcc
    13621 tcaatacgac aacctcgacc gtccgttgcg ccctgagcgc ggcctgttgg caatccgtaa
    13681 ggatttgaac ctgtttgcca atttgcgtcc tgctgtttta tatcccgaat tagccaacgc
    13741 ctccacgctg aagcctgaaa tcgttgccgg tttggatatt ttgattgtgc gcgaactcac
    13801 cggcgacatc tatttcggcg aaccgcgcgg catccgcgtt ctggaaaacg gcgaacgcga
    13861 aggctacaac accatgaaat acagcgaaag cgaaatccgc cgtattgccc atgttgcctt
    13921 ccaatccgcc caaaaacgca gcaaaaaagt ctgttccgta ggcaaagcca acgttttgga
    13981 aaccaccgaa ttgtggcgcg aaatctttga agaaatcggc aaagagtacc ctgatgtcga
    14041 gctttcccat atgtacgtcg acaacgccgc catgcagctc gtgcgtgcgc ccaaacaatt
    14101 cgacgtcatt gccaccggca acatcttcgg cgacattctc tccgacgaag cctccatgct
    14161 gaccggctcc atcggtatgc tgccttccgc ctcgctggac gaaaacggca aaggcctgta
    14221 cgaaccgtct cacggctccg cccccgacat cgccggacaa aacaaagcca acccgctggc
    14281 caccatcctc tcgctcgcaa tgttgctgcg ttacagcctg aacgacgaag cgcgcgcgca
    14341 acaagttgaa aacgccgtcc aaaaagtgct gcaacaaggc ttgcgtacca gcgacattta
    14401 cgaagaaggc acaaaactcg tttcctgctc cgaaatgggc gatgcggtac tcgccgcctt
    14461 gtaaaaaaac aaagcgcgga tcacaccgcg ctttttcatc cgcccttttc cgcccgcccc
    14521 gttcgtcaac cgcctgcaaa cccaccgttt accggcagga aatgccataa atccctgcct
    14581 atgttaggat aaacgccttt accggcccac acaaaggatt atcatcatga aaaaaatcat
    14641 cttcgccgca ctcgcagccg ccgccatcag tactgcctcc gccgccacct acaaagtgga
    14701 cgaatatcac gccaacgccc gtttcgccat cgaccatttc aacaccagca ccaacgtcgg
    14761 cggtttttac ggtctgaccg gttccgtcga gttcgaccaa gcaaaacgcg acggtaaaat
    14821 cgacatcacc atccccattg ccaacctgca aagcggttcg caacacttta ccgaccacct
    14881 gaaatcagcc gacatcttcg atgccgccca atatccggac atccgctttg tttccaccaa
    14941 attcaacttc aacggcaaaa aactggtttc cgttgacggc aacctgacca tgcacggcaa
    15001 aaccgccccc gtcaaactca aagccgaaaa attcaactgc taccaaagcc cgatggagaa
    15061 aaccgaagtt tgtggcggcg acttcagcac caccatcgac cgcaccaaat ggggcatgga
    15121 ctacctcgtt aacgttggta tgaccaaaag cgtccgcatc gacatccaaa tcgaggcagc
    15181 caaacaataa atcttgatgc gaatcagcac aaatgccgtc tgaacgtcct tcaaacggca
    15241 tttgtgccgt cttcatcaaa ccgccccgcc aacggtaagc cgatatggcg gagcacaatc
    15301 cgccgaaaga tatttcagcg gcaggcaggg cttgccggaa agtccggaaa
//
LOCUS       DAXHMX010000034        15325 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHMX010000034 DAXHMX010000000
VERSION     DAXHMX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.034"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..5437)
                     /gene="hrpA"
                     /locus_tag="ACFM4S_000791"
                     /pseudo
     CDS             complement(1..5437)
                     /gene="hrpA"
                     /locus_tag="ACFM4S_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
     gene            complement(5546..7225)
                     /gene="hrpB"
                     /locus_tag="ACFM4S_000792"
     CDS             complement(5546..7225)
                     /gene="hrpB"
                     /locus_tag="ACFM4S_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HFC6050517.1"
                     /translation="MEIINDAELIRSMQRQQHIDAELLTDANVRFEQPLEKNNYVLSE
                     DETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTGMCLGSNNLSRLQKAAQQILIV
                     RGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEKRDGKSAEGSISAFNNKFPLYR
                     NKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKSDLQIKWQQNKPIRFSIGIDDA
                     GGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHKTDLTDATGTETASGSRSYSVH
                     YSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQYQSSLAAERMLWRNRLHKTSVG
                     MKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYLNRWQLDGKLSYKRGTGMRQSM
                     PAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQFFYATAIQAQWNKTPLVAQDKL
                     SIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNHQFYLGADYGRVSGESAQYVSG
                     KQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQTTNTVYGFNLNYSF"
     gene            7172..8155
                     /gene="repM"
                     /locus_tag="ACFM4S_000793"
     CDS             7172..8155
                     /gene="repM"
                     /locus_tag="ACFM4S_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="HFC6050518.1"
                     /translation="MLLTLHGTDKFCIVNNFHTNRNTAYKALKDTCNNLFERQFSFIE
                     KTPKGEKVVRTRWVSQVAYIEQQATVELVFAPNVAPLITMLEKNFTSYELDQVSSLSS
                     KYAVRLYEIIISWRAAGKTPMFSTMELRERLGMMPDEYQKMELFKRKVLDFAVKQIND
                     KTDISITYEQHKEGRKIVGFTFSILHKIGSKDIPLENQSELFAGMTDLEAGTIRVRAE
                     AYIASLIAKGQNVTKAHRLNILKKAVEERWGFEDVAKDNGAKNPENKNAKVVLNEWEK
                     ISNGTRFKDKDGTIWVKDSGMLRTEGTNRWIADSQIAKLFPMLTVMVEEGI"
     gene            8252..8668
                     /locus_tag="ACFM4S_000794"
                     /pseudo
     CDS             8252..8668
                     /locus_tag="ACFM4S_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
     gene            complement(8674..8772)
                     /locus_tag="ACFM4S_000795"
                     /pseudo
     CDS             complement(8674..8772)
                     /locus_tag="ACFM4S_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            8865..>15325
                     /locus_tag="ACFM4S_000796"
     CDS             8865..>15325
                     /locus_tag="ACFM4S_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6050519.1"
                     /translation="MNRTLYKVVFNKHRNCMIVVAENAKREGKNTADTQAVGILPNDI
                     AGFAGFIHSISVISFSLSLLLGSALILTSSSANAQGIVADKSAPAQQQPTILQTGNGI
                     PQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSRSNTQTQLGGWIQGNPWLARGEARV
                     VVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTTGQPQYQA
                     GDLSGFKIRQGNVVIAGHGLDARDTDFTQILSHAVKIDGPVWGKDVRVSAGKNDVSAD
                     GSIRSSHSPATNINSDNSLYAIDTGALGGMYAGKITLISTDRDASVRNQGQWFASAGN
                     VAVNAEGKLVNTGMIAATGENHAVSLHARNVHNSGTVASQDDANIHSQTLDNSGTVLS
                     SGQLTVRNLGRLKNHNNGTIQAARLDMSTGGLDNTGNITQTGSQALDLVSAGKFDNSG
                     KIGVSDVPQTGLNPNPSVIPQIPSTATGSGSSTVLASKPGSNNPVSPTAPAKNYAGGR
                     IQTTGAFDNAGSINAGGQIDIAAQNGLENSGSLNAAKLRVSGDSFNNTVKGKLQAHDL
                     AVNTQTAKNSGHLLTLTGKIDNRELHNAGEIAANNLTLIHSGRLSNDKKGNIRAAHLQ
                     LDTAGLHNAGNILADSGTVTTKNNLRNTGKVSVARLNTEGQTLDNTHGHIEAETVNIQ
                     SQQLTNQSGHITATEQLTINSRNVDNQNGKLLSANQAQLAVSDGLYNQHGEIATNRQL
                     SIHDKNQNTLALNNADGTIQSAGNVSLQAKSLANNGTLTAGNKLDIALTDDFVVERDL
                     TAGKQLNLSIKGRLKNTHTLQASHTLKLNAGNIDNQVTGKIIGGEQTDITSEQHVDNR
                     GLINSDGLTHIGAGQTLTNTGTGKIYGNHIALDAQILLNREETTEGSTKAGAIAARKR
                     LDIGAKEIHNQEGALLSSEGIFAVGNRLDEQHHAAGMADTFVNGSAGLEVQGDALMSV
                     RNMQNINNHFKTETYLAKAEKQVRDYTVLGQNTYYQAGKDGLFDNSQGQKDQTTATFH
                     LKNGSRIEANQWHVRDYHIETYKERIIENRPAHITVGGDLTASGQNWLNKDSRIVVGG
                     RIITDDLNQKEITNQSTTGKGRTDAVGTQWDSVTKKGWYSGRKRQRRTERNHTPYHDT
                     QLFTHDFDTPVSVIQQNAASPSFQPAASAIKLIDGVSTAAVNGQRIHTGNVVSLNNAT
                     VTLPNSSLYTTHPDNKGWLVETDPQFADYRRWLGSDYMLQQLQLDTNHLHKRLGDGYY
                     EQKLVNEQIHQLTGYRRLDGYRSDEEQFKALMDNGLTAAKTFGLTPGIALSAEQVARL
                     TSDIVWMENQTVTLSDGSTQTVLVPKVYALARKGDLNTSGGLISAEQVLLKLQNGNLT
                     NSGTIAGRQAVLIQARNINSNGNIQADQIGLKAEKSINIDGGQVQAGRLLTAQAQNIN
                     LNGTTQTSGNERNGNTAIDRMAGINVVGSHTEQVDNRTSDGILSLHAGNDINLNAATV
                     SNQVKGGTTQITAGNNLNLGTIHTEHREAYGTLDDENHRHVRQSTEVGSSIRTQNGAL
                     LRAGNDLKIRQGELEAEEGKTVLAAGRDVTISEGRQITELDTSVSGKSKGILSSTKTQ
                     DRYRFSHDEAVGSNIGGGKMIVAAGQDINVRGSNLISDKGIVLKAGHDIDISTAHNRY
                     TGNEYHESKKSGVMGTGGLGFTIGNRKTTDDTDRTNIVHTGSIIGSLNGDTVTVAGNR
                     YRQTGSTVSSPEGRNTVTAKSIDVEFANNRYATDYAHTQEQKGLTVALNVPVVQAAQN
                     FIQAAQNVGKSKNKRVNAMAAANAAWQSYQAAQQMQQFAPSSSAGQGQNNNQSPSISV
                     SITYGEQKSRNEQKRHYTEAAASQIIGKGQTTLAATGSGEQSNINITGSDVIGHAGTA
                     LIADNHIRLQSAKQDGSEQSKNKSSGWNAGVAVKIGNGIRFGITAGGNIGKGKEQGGN
                     TTHRHTHVGSTAGQTTIRSGGDTTLKGAQLIGKGIQADTRNLHIESVQDTETYQSKQQ
                     NGNVQVTVGYGFSASGSYRQSKVKADHASVTEQSGIYTGEDGYQIKVRDNTDLKGGII
                     TSTRSAESKGKNRFQTATLTHRDIQNHSRYEGRSFGIGGSFDLNGGWDGTVTDKQGRP
                     TDRISPAAGYGSDGDSKNSTTR"
BASE COUNT         4067 a         3729 c         3674 g         3855 t
ORIGIN      
        1 gttaccgcac ccgtggcgat tcccgcgccc gcttcggcag cctgagtgac catgacagta
       61 caaccagaag gattagccat acaggtgctg atagctaatt tacccgctgt accgatcagc
      121 ggagctgtcc aacctgctgc ataaactcca tagctggtaa tcacaatcgg gcctgtgatg
      181 ccattacgga tattgcttat ccaaatggca gcatccttat cctgcggatt agtcatcgca
      241 cctgccgcat gtgcaggcat aatactttgg atgatttttt ccagtgcggt tttgtcgggc
      301 ttctgcggtt gatgcttttt cgcattggta ggggtgctgt caaaattcaa agcattattc
      361 accacagcta cctcagccgc attcgccgca gtattcacat cgccgccgtt gagtgccgcc
      421 acgctgcccg caataatctt cgagtaactg ataactttat gcttttctgc atcgctgagt
      481 gtagcagggt ttctgccgcc aagtatggat tcagctacga tttccccaac tgctgcgcca
      541 attgccccat ctttacattt tccctgtacc agtccgctaa cacaacccgc caaagcgtgg
      601 gcgaactgtt tggcaacata atcgtcgctg aaggttgttt tgattttgct ggcggcttcc
      661 ccttggaagc tattaaccaa tgctcccaat gcggcattgc ctaagttgtc tttcaagctg
      721 ccgccgttaa cggcggtatt gataccagct gagatacctg cattgctgag gttggtggcc
      781 aatctgcctc caaggttggc aatggtttga ttgcccgtac tgctgaacag ttcggttctt
      841 accttgctgt tcaattgggc aatatctgct cccatctggt ttaatgcacc cgccgtcagt
      901 gcggaagtaa caatctgctt gaccgtatca ctggtgccga gatcttttaa tgctttgcct
      961 acatcgcctt tattattgat gatggctaca gctgcttggc tatacaagga ggctaaagct
     1021 gcggtttgca tggcagctgc agtagaaaca gtggtagttg ctgccgctgt cgttgttgca
     1081 gctgcggctg tactacttcc tgcggcagtt acgctacccg ctgcagccgc tccgtatcca
     1141 taagttagag cggttacaat aatcaccaca atcgctgcac cggctccggt caagccttcc
     1201 tgcttatagt cccatttgtc gtacgccagc tgtacctgtt tccaatcgac gttcttggcc
     1261 gtctgaagct gtttcaggta ggcgtattcg ggctgtttgg ccagcttttc gatttcggtt
     1321 ttcagattgc ctttgggaat gtcgacgata tagccgccgg gtgcggacaa tttgggcgga
     1381 gtagggcttt cgaagctggg cagttttagc gtttcgatag tgctgccgcg tccggcctgt
     1441 ttctgccata cggttgagtt ggtttctaat ttttcttccg actggatacg gttcacaatg
     1501 cctttgagga taattttcgc atcgacacgg gctttttcgc ctacacctgc ctgaatgtcg
     1561 gcaccggcca gcgtggtttt gaattcggta ccttcgagca cggtatccca gcctgaacgg
     1621 gtggctgcag tttgggcgac gacgcggaca ggcaatttgg tttcgttcag ttcgttttta
     1681 ctgtaattgc tcttacctac cttgatgccg ataaagcggc ggcttttttg gacatccaac
     1741 tcgtgcttgt ggatgccttc ttctgccagc agttgcagct cttcacccgc aaccagggta
     1801 actttacctg caggggcatt gaagcgggtg gtattagctt cgatgttgcc gcctgcctga
     1861 agcgtgatac cgttggcggt cagctcgacg ggggctggca taatcaggtg gtcgcgggtg
     1921 ctggtaaact tggtttttct gatgattttg ccgcttttac ctttggtttt taagaaggta
     1981 taggcatcgt tttgtccagc ctccagtaca atatcactat gggctttgat gtctatgctg
     2041 cctgagggag ctttgatttc ggatgcaccg ataataatac gtgcatcatc gagtgccgca
     2101 gctgcatgaa tacttacccc cgtacgtccg gtcaaacgtg aaggcttgtt cagagcagct
     2161 ttgtcgtagt gactcttgta ggtgggcttg ccaatttcat attggtcggt tatgccgtca
     2221 atcagaatag cagccgcctc tgaatctgct gcctttggca atacgcctgc ggcgtgaagg
     2281 ttcagttttt tggaagcggt aatatcggaa ccgctgattt cgatgccttg tgcggaaatc
     2341 aagtcaatat tttgtgcaga aagcttggct tgcagatatt ctttgccttt gggtttttta
     2401 cctttaactt ccttgttgat ggcttgaata tagaaagcga gacggtcgcg ttcttcttgc
     2461 agggttggaa tcagcttgct tttaggcgag cttttttcaa ctgcgcaatc tgctgttcca
     2521 attctttgga tttttggttg agttcagccg ctttttgtgt aggaaaataa ttgctgaatg
     2581 agttgtttac ggcttcgata ttcaacttgc ctttggtggt ggcgacaacc aagtttttac
     2641 cggctgtaat tttagaacct cttaaatctg tttctcctgt aaccagacgg atattgcctt
     2701 ttgcttccaa tgaggaaact tgagcactag gcgcacctgc atttcctcct tttacagaca
     2761 acagcaattt tccgcctgtt ttgatgctca ggtcggtatg cgcactgatg cggttggcag
     2821 gttcgatggt taatgtgccg ctacctgctt caatattcag ccgtccggcc aatggtttta
     2881 attcggcatt atcttccaaa gttttggtcg aaacggtact ccaattgatg ttgccgcgct
     2941 tgactagggc ggtaccggca gtaaggttga ttgcacccgc tctcagcgtg gtgttgtcgg
     3001 caatttggga atagcgcgca ttgagtgcca atacaccgtt agccaccagc ttgttggcag
     3061 aaggcagttt gtcgttttgc caaatctggc tgccggtaat gcttagatga cggtgtgcgt
     3121 aggcatctac ttggttgagc gttacccgct cgtgttgtgc attaagatgc gtattatggg
     3181 tagactccag cttggtattt tctatgctca atgcccggtc ggaatgaatg ttcaatgccc
     3241 cgcttttggc atggacgtta aggtttttta agtcggcatt accaccgttg tttttgatgc
     3301 tgatgtgttt tccgttgatt gaattgcgtt gttttccgtc accaagctga ataccgttgc
     3361 cggcaaccaa cgtaatatct cctgaagatg aagtgatatt ggtattgtct gctttcagac
     3421 ggcctttacc aaccgatcct gcattgacat cggccttggc tgtcagggta ttgtgaccgg
     3481 taaagtcggc attaccgttg gccaataagg atacatgacc gtctgcagaa acagcatgaa
     3541 ggccgtctga aacgatattg ccctgcaatg cggtggtttc gagagccttg gctgcgttca
     3601 gcttggtatt gcgaagctga atattgcctt tggctgcctg aatgtgcaga ttacccgagt
     3661 tggtacgcag attggtattg gtaacattca gcgagcctgc ctccacaccc atgtcttttg
     3721 aggcagtgag ggttttactg gtgccggtaa tatgggcagc gttatccgat ttcaaatgga
     3781 tgctggcggc agacaaatct ttatcaacat tcaaattcag atctttacct gtatgaacat
     3841 acagattgcc gggagtattc agattggtag ttttggcagt aatgttatcg tctgccagta
     3901 aagcaagctg cttgccgccc ttgatactgc ctccgtttaa gcggatatcg gaggtaactg
     3961 ttgaagcttc caaagaaagc ggtttgcctg cttcgatgtg tgcggtgtct ttggcatcta
     4021 ttacggcgga actgctgatg gtgccgttgg ataatacggt aacatctgcc ccggtaatgc
     4081 gtgtgttatt gcctaattcg gcgttgcctt tgctggaact gtatacggta gtgccagtct
     4141 gaatactggc ctccttgatg acggtacggc cgccggtcga cagattggtc gaacctttag
     4201 cattgttcac atttgctttg ctttcgataa ccaaattacg gccggcattt aataccgtag
     4261 cagcagggcg gttgccgttg ttttgaacga ccgctccgtt acgcaaaccg atgtcctccc
     4321 cggtatcaat aaccaataag cctttgctct cgatccgacc atcattggag ataaatgtgc
     4381 ctgccgctcc tttttcggtg gtttcgatgg agagataagt cggtgaagct tcggtgccgt
     4441 cggcagtggt ggcgatgcgg ccgctgtttt caatgcggcc tgacgaagtc acaatcaatt
     4501 gcttggccgc ttcgagtgtg ccggcatttt tgacgcctac gcctttttca ttggcaatca
     4561 gtgtgatgct gtcggcgtac ataccgccca gtgcggcagt atcaagggca atagtcggtt
     4621 tcgtacccgc tgccgtacct gcactgattt cgccgctggc gtaatctact ttctgaggac
     4681 cggtagaaac cgccaggttt ttaccctgta atttcccctg caaagcaact gcacgagcaa
     4741 gtaccccggt gtagtcggct tcgcctttat cattccaacc tgctgctcct acggtcaatg
     4801 tgccttgacg cacatcaaat cctgtcagtg caccgtcttt gccgatttgg ggcgcaccgg
     4861 tagttaagat gccccgaccg acatttttaa agccgccgcc attaacggta atgccgttgg
     4921 ggttggcaat aatcacgtcg gccttttgac cgcctacggt aacgatgccg ttgagtttgc
     4981 tagccgtacc gcgtacctcg ttcaaaatca attgcgcact gcctttgacc acaaacggat
     5041 tattgttacg gtcgttgttt aacactgccc ctttgttgtc aacatcaaac tgcgtatagc
     5101 ggttgtggct caatccgcgt ccattcggag tttggatatt caccaagggg gcaccagtgt
     5161 tggttttaag gataacgacc tgctggtttt taggtgctga tttgtcggtg gtaatttggg
     5221 catgggcagg caataccata ctcagggaaa ccaaagagca gaccaaagtt ttaagggtgg
     5281 ttttgagttt gccgcaaagg tcgcctgaag ttttcagtga aacagaaacc gaactgcctg
     5341 cctgtttacc tttgccctgg ctgttggcag tttcggctac tgcaaccatg gtgctgtgct
     5401 ttttactaaa gataatgcga tgtaaacctt tattcatgtc tattccattt tgaagatgaa
     5461 cgtactgcgc gccaagtacg taggtaaagt ttgacggtct gaggataagg aaagaccgtc
     5521 taaatatcag taagaaattc agaggttaga aactgtaatt caagttgaag ccgtaaacgg
     5581 tgttggtcgt ctgaaagcct ttgggtttat gaagcggctt gccggcaaac agatcataag
     5641 caaacatacc gcctacttta tgccctcctc tgaagccgac cactgcaccc atcagctgct
     5701 tgcccgatac atattgtgca ctttcgccag atacgcggcc atagtccgca ccgagataga
     5761 actgatggtt cggatgaaaa taccaagtta aagtattctg ccagtagaaa cctcgctctc
     5821 cgaaaagact ctgctcccca tcaaatccgc gaacggtgta gcggctgccg attgacaatt
     5881 tatcttgggc aaccaacggc gttttgttcc attgagcttg aatggcggtt gcgtagaaaa
     5941 actgctgttt gcccaacata aacggggccg ctgcatccaa tccggcggtt atgattttca
     6001 tacgggatgt gcctggaata gtaccgccgc cgttttcttc aggtgcgggc atactttggc
     6061 gcataccggt cccgcgtttg taagacaact tgccgtcaag ctgccaacgg ttgaggtaag
     6121 cacggtggcg caattcggct tcccagcctg cagagcggcg gcgttgcact tcgatttcgg
     6181 catcgtcgat gtatttatag gtttggcgtg tccataattt cattccgact gaagttttat
     6241 gaagtctgtt acgccaaagc atgcgctcgg cggccaagct gctctgatat tgtttgccgt
     6301 tgtaatcgta attgacggaa tagccttcgg ttgcttcgtg gtaacgatgt ccattgtgat
     6361 taaaagaaaa cagccatttt tttacgggca ccgaataatg cacgctgtaa cttctggatc
     6421 cgcttgcagt ttccgtaccg gtggcatcag tcaagtccgt tttgtgcgcc aaaccgcgtc
     6481 catatgaaac ataaaacaaa tcgcttaagc ccaaagggtt atcgaacgat aaagcgacat
     6541 ttccttgata tttgccggtc gttttgccgc ccgcatcatc tataccgata ctgaaccgta
     6601 tgggtttatt ctgctgccat ttgatctgta aatcgctttt gccttcttct tcggacggta
     6661 taatctgaat atctgtttta acactcgaca aacgacgcag gttttccaag ccctgctcta
     6721 catcgcgaag attgagaatt ttgttcctat ataagggaaa tttgttattg aatgcactaa
     6781 tactgccttc ggcagacttc ccatcccgtt tttcttcata gcggatatcc cctatttcgc
     6841 ctgctgatac ccgtaatttc agaattcccg aatccatatt ctgtggttgg ataatagctt
     6901 gggaagtgag gtagccacgc acaatcagta tctgttgcgc ggctttttgt agcctgctca
     6961 aattattgga acctaaacac atcccagttt taaaagctgt ttctttcatg agcacagaag
     7021 gaagaaaaga aaatttgcgc gccgtcttat catctaaact aatgtaattt acccgagtac
     7081 acggtgtttc atcttcactc aggacataat tgttcttctc caatggttgc tcgaaacgga
     7141 catttgcatc agttaacaat tcagcatcta tgtgctgctg acgctgcatg gaacggataa
     7201 gttctgcatc gttaataatt tccataccaa ccgtaatacg gcgtataagg ctttgaaaga
     7261 tacctgtaac aacctttttg aacgccaatt cagttttatc gaaaaaacac caaaagggga
     7321 aaaggtagta cggacaaggt gggtatctca agtcgcctat attgaacaac aggcaacggt
     7381 agagttagtt tttgcaccaa atgttgcccc tttgattacg atgctagaaa aaaacttcac
     7441 aagctacgag cttgatcagg tctcatcgtt gagcagtaaa tacgcggtgc ggctctacga
     7501 aattattatt tcatggcgtg cagccggtaa gacaccgatg ttcagtacaa tggagttgcg
     7561 cgaacgtttg ggtatgatgc ctgacgagta tcaaaaaatg gagctattca aacgtaaggt
     7621 tttggatttc gccgtcaagc agatcaatga taaaacggat atttccatta cctacgagca
     7681 gcataaagaa ggacgaaaaa ttgtaggttt tacattctca atcctacata aaatagggtc
     7741 gaaagacatc cctcttgaaa atcaatcgga actttttgcc ggaatgactg atttggaagc
     7801 cggaacgata cgggtcaggg cggaagcata tatcgcttcg ctcattgcaa aaggtcagaa
     7861 cgtgactaaa gcccataggc tgaatattct gaaaaaagcc gtagaggaac gttggggatt
     7921 tgaagatgtg gctaaagata atggggccaa aaatcctgaa aacaaaaatg caaaagtcgt
     7981 tttaaacgaa tgggaaaaga tttctaatgg cacacgtttt aaggacaagg atggaacaat
     8041 ttgggttaaa gattcaggta tgcttaggac tgaagggaca aacaggtgga tagccgattc
     8101 tcagattgcc aaattatttc ctatgttaac tgtgatggtt gaggaaggta tttaacccgg
     8161 caactcgcgt attctacctg ttttgcggta cggaaaccaa ttgaacctgc tttacgctac
     8221 aatagaagat tgcaattttg tcggatgtac catgaacgat tacacagcca tgccgtctga
     8281 agacggagga atcggctcat tatcgcttcc gccgcactca atggaggcgg aacaatccgt
     8341 tttgggcggg ttgatgctgg aaaatccggc ttgggacagg attgccgatg tggtttcggg
     8401 agaggatttc taccgccacg aacaccgcct gattttccga tccattgcca aactgatcaa
     8461 tgagagccgt cctgccgatg tcatcacggt tcaggaagat ttgcagcgga acgaagagct
     8521 ggaagcggcg gggggattcg aatatctgat tacgctggcg caaaatactc cgtctgccgc
     8581 caacatccgg cgccatgccg aaatcgtgcg cgagcgttcc attatgcgcc aactcgccga
     8641 agtgggaacg gaaattgccc gcagcgccat acccaaaata tcggcagcgg aacgggcggt
     8701 agcttggagt acgaaaaatt caagcagttt cttttgaact ctattgttta atttacaatt
     8761 ggttatcttt attttcgtag cctggcataa ctgttaatct acgtcagccc ctaaaaatta
     8821 atctaattta cgattataaa aatattatta agaaagccct tactatgaac cgcaccctgt
     8881 acaaagttgt atttaacaaa catcgaaact gcatgatagt cgttgctgaa aatgccaaac
     8941 gcgagggcaa aaacacagcc gacacccaag ctgtaggtat tttgccaaat gatattgcgg
     9001 gctttgcggg ttttatccat tctatctctg ttatctcatt ctccctttca ttactgctcg
     9061 gttctgccct tatcctgact tcttcttctg ctaatgccca aggtatcgtt gccgacaaat
     9121 ccgcacctgc acagcaacag cctaccatcc tgcaaacagg taacggcata ccgcaagtca
     9181 atattcaaac ccctacttcg gcaggggttt ctgttaatca atacgcccag tttgatgtgg
     9241 gtaatcgcgg ggcgatttta aacaacagtc gcagcaacac ccaaacacag ctaggcggtt
     9301 ggattcaagg caatccttgg ttggcaaggg gcgaagcacg tgtggttgta aaccaaatca
     9361 acagcagcca ttcttcacaa atgaatggct atattgaagt gggcggacga cgtgcagaag
     9421 tcgttattgc caatccggca gggattgcag tcaatggtgg tggttttatc aatgcttccc
     9481 gtgccacttt gacgacaggc caaccgcaat atcaagcagg agaccttagc ggctttaaga
     9541 taaggcaagg caatgttgta atcgccggac acggtttgga tgcccgtgat accgatttca
     9601 cgcagatatt gagccatgcg gttaagattg atgggcctgt gtggggtaaa gatgtgcgtg
     9661 tgtcggcagg gaaaaatgat gtctcagctg atggctctat ccggtcttca cactcccctg
     9721 ctacgaatat aaattccgac aactcactgt atgccatcga tacaggggca ttgggcggta
     9781 tgtatgccgg taagatcacc ctgatcagca ctgatcgtga tgcatccgtg cgcaatcagg
     9841 ggcaatggtt tgcctcagcc ggcaatgtgg cagtgaatgc tgagggtaaa ctggtcaaca
     9901 caggcatgat tgcagcgacg ggagaaaatc atgcggtttc acttcatgcc cgcaatgttc
     9961 ataatagcgg tacggttgcc tcacaggatg atgccaatat tcacagccag acgctggaca
    10021 attcaggtac ggtcttatcc tcaggtcaat tgactgtccg taatttaggc cgtctgaaaa
    10081 accataacaa cggtacgatc caggctgcac gcttagatat gtcaacaggt ggtttggata
    10141 acacaggtaa tattactcaa acaggttcac aagcattgga tttggtatct gcaggcaaat
    10201 tcgataacag tggcaagatt ggtgtaagtg acgttccaca gaccggtttg aatcccaatc
    10261 catcagtcat accacagatt ccgagtactg caacaggttc aggcagcagc actgtcttgg
    10321 catctaagcc tggttcaaac aatcccgttt cacctacagc acctgcaaaa aactacgccg
    10381 gaggacgcat tcaaacaaca ggagcatttg acaatgcagg atcaatcaat gcaggtgggc
    10441 aaattgacat tgccgcccaa aacggtttgg aaaattcggg tagtctgaat gcggctaaac
    10501 tacgagtatc aggcgattca tttaacaata cggtaaaagg caaactccag gcacacgatc
    10561 tggctgttaa cactcaaact gctaaaaaca gcggtcactt attaactcta accggcaaga
    10621 ttgacaaccg tgaactgcat aatgccggag aaattgccgc caacaatctg acactcattc
    10681 attcgggccg cttgagcaat gataaaaaag gcaatattcg agctgcacat ttacagcttg
    10741 ataccgccgg tttacataat gcaggtaaca ttcttgccga tagtggaacc gttaccacca
    10801 agaataatct tcgcaataca ggaaaagttt ctgttgcacg actaaatacc gaaggtcaga
    10861 ctctagataa tacgcacgga catatagagg ctgaaacggt taacatccaa agtcagcaac
    10921 tgactaacca aagtggccat attactgcta ccgaacaact gactatcaat agtcgaaatg
    10981 tagacaacca aaacggcaaa ctcctatctg caaaccaagc acaattagct gtttcagacg
    11041 gcctatacaa ccaacatggt gaaattgcca ccaaccggca gttgtctatt cacgataaaa
    11101 atcaaaacac tttggcgtta aacaatgcgg atggcacgat tcaatctgcc ggtaatgtat
    11161 cgctacaagc caaatcactc gccaacaatg gcacattaac agccggtaac aaactggata
    11221 ttgctttgac ggacgatttc gtcgtagagc gcgacctcac tgcaggcaaa caattaaatc
    11281 taagcataaa aggccgtctg aaaaataccc ataccctaca agcaagccat acgctcaaac
    11341 tcaatgccgg caatatagat aaccaagtta caggcaaaat tattggtgga gaacaaacgg
    11401 acatcacatc cgaacagcat gttgacaaca ggggcttgat caacagcgac ggtttgaccc
    11461 acatcggtgc aggtcaaacc ctgaccaaca ccgggacagg caaaatctat ggcaaccata
    11521 ttgccctgga cgcgcaaata ctgcttaacc gggaagaaac gacggaaggc agtaccaaag
    11581 cgggggcaat agctgcaagg aaacgtttgg atattggagc gaaagagatt cataaccaag
    11641 aaggtgccct actatccagc gaaggtattt ttgccgtagg taatcgactg gatgaacaac
    11701 atcatgcggc aggcatggcc gatacctttg ttaatggcag tgccggtttg gaagtacaag
    11761 gtgatgcatt gatgtccgtt cggaatatgc agaatatcaa taatcacttt aaaacagaga
    11821 catacttagc caaagcagaa aagcaagttc gtgactacac cgtactgggg caaaatacct
    11881 actatcaggc aggaaaagac ggtttattcg acaactcgca aggacaaaaa gaccaaacta
    11941 ctgctacgtt ccatttaaaa aatggttctc gtattgaggc caaccaatgg catgtccgag
    12001 actaccacat cgagacttat aaagaacgca tcatcgaaaa ccggccggca cacattactg
    12061 tgggcggtga tttgactgcc tcaggtcaaa attggctgaa caaagacagc cggattgtag
    12121 taggcgggcg tattatcact gatgatttaa accagaaaga aattaccaat caaagtacaa
    12181 caggcaaagg tcgcacagat gctgtcggca cacagtggga ttcagttaca aaaaaaggat
    12241 ggtacagcgg tagaaaaaga caacgccgta ctgaaagaaa ccatactcct taccatgata
    12301 cccaactatt tacccacgac ttcgacacgc ctgtatccgt catccaacag aatgccgcct
    12361 ccccttcctt tcaacccgct gcatctgcaa tcaaactgat tgacggagta tccacggcag
    12421 ccgtcaatgg tcagcgcatc cataccggta atgtggtctc gttaaataac gctactgtta
    12481 ctctgcctaa cagcagcctc tataccaccc atcctgacaa taaaggctgg ttggttgaaa
    12541 ccgatcctca atttgcagac taccgccgct ggttgggcag cgactacatg ttgcaacaac
    12601 tgcaattgga caccaatcat ctacacaaac ggctaggcga cggctactac gaacaaaaac
    12661 ttgttaatga acaaatccat cagttaacag gctaccgccg actcgacggc tacaggagtg
    12721 atgaagaaca attcaaagct ctgatggaca acggccttac tgctgccaaa acattcggtc
    12781 tcaccccagg tatcgccttg agtgcagagc aagttgcccg cttaacttca gatatcgttt
    12841 ggatggaaaa tcaaaccgtc accctgtctg acggttcgac tcaaaccgta ctggttccta
    12901 aagtctatgc cctggcgcgc aaaggtgatc tcaatacctc cggtggcctg attagtgccg
    12961 aacaagtctt acttaaactg caaaacggca acctgactaa cagcggtacc attgcggggc
    13021 gacaggccgt actcatccag gcacggaata ttaacagcaa cggtaacatt caagccgacc
    13081 aaatcggctt aaaagctgaa aaaagtatca atatcgacgg cgggcaggta caagcaggca
    13141 gactgctgac tgcccaagcg caaaatatca accttaacgg tacaacccaa acttccggta
    13201 atgaacgtaa cggcaatacc gccatcgatc gtatggccgg cattaacgtg gtcggaagcc
    13261 atactgagca agtagataac agaacttcag acggcatcct atccctgcat gccggcaacg
    13321 atatcaacct caatgcggcc accgtctcta accaagttaa aggcggcact acccaaatta
    13381 ccgccggcaa taatctcaac ctcggcacca ttcataccga acatcgcgaa gcctatggta
    13441 cattagatga cgagaaccat cgccatgtcc gccaaagtac cgaagtcggc agcagtatcc
    13501 gcacgcaaaa cggcgcactg cttagagccg gtaacgactt aaaaatccgc caaggcgaac
    13561 tggaggccga agaaggcaaa accgtccttg ccgcgggacg tgatgtcact atcagcgaag
    13621 gacgccaaat aaccgaactg gatacctcgg taagcggaaa aagcaaaggc atcctttcca
    13681 gtaccaaaac acaggatcgc taccgcttca gtcatgatga agcagtcggc agcaacatcg
    13741 gcggcggcaa aatgattgtt gcagccgggc aggatatcaa tgtacgcggc agcaacctta
    13801 tttctgataa gggcattgtt ttaaaagcag gacacgacat cgatatttct actgcccata
    13861 atcgctatac cggcaatgaa taccacgaga gcaaaaaatc aggcgtcatg ggtactggcg
    13921 gattgggctt tactatcggt aaccggaaaa ctaccgatga cactgatcgt accaatattg
    13981 tccatacagg cagcattata ggcagcctga atggagacac cgttacagtt gcaggaaacc
    14041 gctaccgaca aaccggcagt accgtctcca gccccgaggg gcgcaatacc gtcacagcca
    14101 aaagcataga tgtagagttc gcaaacaacc ggtatgccac tgactacgcc catacccagg
    14161 aacaaaaagg ccttaccgtc gccctcaatg tgcccgttgt ccaagctgca caaaacttca
    14221 tacaagcagc ccaaaatgtg ggcaaaagta aaaataaacg cgttaatgcc atggctgcag
    14281 ccaatgctgc atggcagagt tatcaagcag cccaacaaat gcaacaattt gctccaagca
    14341 gcagtgcggg acaaggtcaa aacaacaatc aaagccccag tatcagtgtg tccatcacct
    14401 acggcgaaca gaaaagtcgt aacgagcaaa aaagacatta caccgaagcg gcagcaagtc
    14461 aaattatcgg caaagggcaa accacacttg cggcaacagg aagtggggag cagtccaata
    14521 tcaatattac aggttccgat gtcatcggcc atgcaggtac tgccctcatt gccgacaacc
    14581 atatcagact ccaatctgcc aaacaggacg gcagcgagca aagcaaaaac aaaagcagtg
    14641 gttggaatgc aggcgtagcc gtcaaaatag gcaacggcat caggtttgga attaccgccg
    14701 gaggaaatat cggtaaaggt aaagagcaag ggggaaatac tacccaccgc cacacccatg
    14761 tcggcagcac agccggacaa accaccatcc gcagcggcgg ggataccacc ctcaaaggtg
    14821 cgcagctcat cggcaaaggc atacaggcag atacgcgcaa cctgcatata gaaagtgttc
    14881 aagatactga aacctatcag agcaaacagc aaaacggcaa tgtccaagtt actgtcggtt
    14941 acggattcag tgcaagcggc agttaccgcc aaagcaaagt caaagcagac catgcctccg
    15001 taaccgagca aagcggtatt tataccggag aagacggcta tcaaatcaaa gtcagagaca
    15061 acacagacct caagggcggt atcatcacct ccacccgaag tgccgaaagc aaagggaaaa
    15121 accgctttca gacggccacc ctcacccatc gcgacattca aaaccacagc cgctacgaag
    15181 gcagaagctt cggcataggc ggcagtttcg acctgaacgg cggctgggac ggcacggtta
    15241 ccgacaaaca aggcaggcct accgacagga taagcccggc agccggctac ggcagcgacg
    15301 gagacagcaa aaacagcacc acccg
//
LOCUS       DAXHMX010000035        15325 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHMX010000035 DAXHMX010000000
VERSION     DAXHMX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.035"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            69..323
                     /locus_tag="ACFM4S_000797"
     CDS             69..323
                     /locus_tag="ACFM4S_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050520.1"
                     /translation="MAITACSSTSAPSATEIKVVEKAVMPTPPAALMVAPVRPNPPKD
                     GKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(368..538)
                     /locus_tag="ACFM4S_000798"
     CDS             complement(368..538)
                     /locus_tag="ACFM4S_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6050521.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            551..769
                     /locus_tag="ACFM4S_000799"
     CDS             551..769
                     /locus_tag="ACFM4S_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050522.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            766..1104
                     /locus_tag="ACFM4S_000800"
     CDS             766..1104
                     /locus_tag="ACFM4S_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050523.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            1116..1382
                     /locus_tag="ACFM4S_000801"
     CDS             1116..1382
                     /locus_tag="ACFM4S_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050524.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            1379..1576
                     /locus_tag="ACFM4S_000802"
     CDS             1379..1576
                     /locus_tag="ACFM4S_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050525.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            1579..2085
                     /locus_tag="ACFM4S_000803"
     CDS             1579..2085
                     /locus_tag="ACFM4S_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC6050526.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(2082..2594)
                     /locus_tag="ACFM4S_000804"
     CDS             complement(2082..2594)
                     /locus_tag="ACFM4S_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050527.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDGY"
     gene            2606..4228
                     /gene="terL"
                     /locus_tag="ACFM4S_000805"
     CDS             2606..4228
                     /gene="terL"
                     /locus_tag="ACFM4S_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC6050528.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAVSARRAKVFD
                     PIGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWRGVKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIVIGGYQRSMG
                     RLYVVEAQIKKRLPDLIIEDVICLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFGNEIKSKWSR"
     gene            4228..5796
                     /locus_tag="ACFM4S_000806"
     CDS             4228..5796
                     /locus_tag="ACFM4S_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFC6050529.1"
                     /translation="MAKKDNKTKIQKPEAALQTDAAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDDGLLLRTRENPEGEALWPLGWVVHTQKSRSVRQARNGLFRSLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTTLAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            5783..7078
                     /locus_tag="ACFM4S_000807"
     CDS             5783..7078
                     /locus_tag="ACFM4S_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFC6050530.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDSYPTTAYKAPDGSLYTADKGFGYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            7188..7604
                     /locus_tag="ACFM4S_000808"
     CDS             7188..7604
                     /locus_tag="ACFM4S_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFC6050531.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            7835..8896
                     /locus_tag="ACFM4S_000809"
     CDS             7835..8896
                     /locus_tag="ACFM4S_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFC6050532.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPEGAALSADVFAQLAEK
                     DSRIAALSAQNGTPDLTRYAPVSLVRDLQNQVAALTAEREADKGGELITAALSAGKLL
                     PAQKEWAEGVLKQPGGFAFLSGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            8958..9467
                     /locus_tag="ACFM4S_000810"
                     /pseudo
     CDS             8958..9467
                     /locus_tag="ACFM4S_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9457..10602
                     /locus_tag="ACFM4S_000811"
     CDS             9457..10602
                     /locus_tag="ACFM4S_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFC6050533.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            10599..11267
                     /locus_tag="ACFM4S_000812"
     CDS             10599..11267
                     /locus_tag="ACFM4S_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFC6050534.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            11368..11715
                     /locus_tag="ACFM4S_000813"
     CDS             11368..11715
                     /locus_tag="ACFM4S_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFC6050535.1"
                     /translation="MDKELNPSIGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            11729..12784
                     /locus_tag="ACFM4S_000814"
     CDS             11729..12784
                     /locus_tag="ACFM4S_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFC6050536.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLRRRNPTTASGTLTVSGIAQSMLSDG
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADELGAAANVRDGEAQLMAAPAGVSTECR
                     LTVQGGTDRESDASLLARLLEIIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLG
                     TVDIAITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGID
                     LDEAKRRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVN
                     RIEWFKAGAINVTEMPS"
     gene            12781..13341
                     /locus_tag="ACFM4S_000815"
     CDS             12781..13341
                     /locus_tag="ACFM4S_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFC6050537.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            13352..15325
                     /locus_tag="ACFM4S_000816"
     CDS             13352..15325
                     /locus_tag="ACFM4S_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC6050538.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAANGLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSALVSTWTDENLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETVNAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRHRDSLLEALQD"
BASE COUNT         3846 a         4101 c         4315 g         3063 t
ORIGIN      
        1 taaaaaggaa atcgaaaatg tccttactca agaccgtaaa aatgcaggcg gcggttgtat
       61 tgacggcttt ggccatcacg gcttgcagct ctacaagcgc gccctcggct acggaaatta
      121 aggttgtcga aaaggcggtc atgccgacac cgcctgccgc attgatggtc gcgccggtac
      181 gcccaaatcc gccgaaagac ggcaagacgg ccacgctgtt ggaacacgcc gccgagtttg
      241 gcggctatgt ggcggagttg gaaaatcaaa atcaggcttg gcgcgactgg gcgggcaatc
      301 actcccgcaa agtcggaaac tgacaaaaaa gcccgcgtag ggcgcgggct gagggtgaaa
      361 gcggatttta tacctctttt acaggggtag cggcggtagt gcttttcagc aaatcgactg
      421 cgtgctgaca gttttgcttg ctggtgtagc cttcgccctg agcgatgatt tcatggttgg
      481 ctgctttcaa acgccaacgg tattcgcctt ttgcgtcttt atagatttca aaatacataa
      541 ggtttctcct atgaatgagt acacgttttc ttaccgcttt aacggcaagt cctggtcatt
      601 gagcatttgg gcggacaacc ctgaagaagc cagggcgaaa tttcgggctg cacgagaaaa
      661 tgcgcactat gacggcgaag ttgtagcaaa ggtttataca tttgtaaata tttcgtgggt
      721 taagaaattg tacaagcgga caaaatattt aatgggtatc aaagaatgac ctaccgtgaa
      781 ttagttgaac gtcagttggc tgtgcgccat gccgatttgg aattgggctt aagccgcgca
      841 cgcgagcaag agccgtttgt cattcatgtt tccgatctgt tggataaggc aggcattgag
      901 tacgcggtac gcatggataa ggattttcag acgacgtttc accttgaata tccaattacg
      961 aactatgaca cctttaaacg tgcggtttgg caaactttgg gggcgtatta ctgtgtttgt
     1021 aatgatggtg atggactgga gattgccagc aatcgccctg acggttacgc cgtccgtatc
     1081 gtattcggcg atgtgccggt ttaaaggggt tttaaatgga ctttgaattt ggtttcagaa
     1141 ccctgtggcc gattgcgacg gcggcatttt ggttttgggt caacggcatt tcaggccgcc
     1201 tgaaagaggc ggacaagcgt atcgacgacc ttaaagagga gttgcacgcg gtcaagctct
     1261 cttatcacac caaggcggac gccaaggcag acagcactaa tattgcggcg gccttggagc
     1321 gaattgaaaa caagttagaa aaagtaaacg aaaaactgga caggaaagca gacaaatcat
     1381 gagcgacccg attttggaag ccttggcgcg tattgaaaac aagactgatc aaacgctgaa
     1441 aaatcagaaa gaaatgcagg cggaaattgc gcaaatccgc caagacacga aacgcacggc
     1501 cattacattc ggcgcactgg gcggcggcgt gattacggtc ggctgggaat tgcttaaagc
     1561 gaaaatggga ctgtaattat ggctcacccg caagaaatcc gtgaaaagtt acgtcggctc
     1621 tatgtgagcg gcgagcaaac tttggaaacg gcggccttga tgtgcgaaat cccgcagacc
     1681 actgcgcgtg cgtggaaacg tgcggataag gaaaaaggcg acgactggga taagatgcgc
     1741 gccgcctaca ctttggccgg cggcggtatt gaggacttga gccgtgcgat gttggccggt
     1801 tttatggtgc agtacaacag cacgatgacg atgctgcagg attcgagtac cgaagatttg
     1861 cccccgtccg accgcgccaa gctgttggcc agcctggccg atgcgtttac gaaaaccgta
     1921 tcggccaatg cgcgtgtgat gccggaaacg tcaaaactgg cgacggcttt ggaattgatt
     1981 gagttcttga tggcgtttgt gcaagaaaaa catcccaaac atttgcctgc ctttgtggag
     2041 gtattggagc cgtttggggc ggaagtggag aagaagtttg gttaatagcc gtctatcaat
     2101 ctcccataac gtgatcctcc tactgaaaat acttcaacat ctgactttaa acgctctccc
     2161 aatattatgg tttggcaacg ggaacaggaa tatttatgaa aagcaccttc atacccattg
     2221 aattgaagaa tcgatttgac accctgctcg taacattggg gacaaatatc atgaattggc
     2281 tcgctgttcg ccatctctgt tttcaatcga tagacgaaag aaccaggagc aagttcatac
     2341 aattggtagc gttgcttctc tgtagaccaa ttttttactg ccaccagttc tttttccaac
     2401 tcatctatac ggctaattgc cgccgtataa gaagattggg cttcaaataa atcagtttga
     2461 ataccagcaa tgatttgatt aagttcgatg gttttttgcg ctacctctgc ctctgttttg
     2521 agctggttaa acccttttaa aagtgatcct gcttctttta ggcctataaa tgcgttagaa
     2581 atgaatccag tcattataaa tactcatgaa aacaaaagaa ttcctcaaat cccttgccga
     2641 actggccgcc agtttgcgcc aagtcatcga agcggaagtg gacggcttcg atgcgtcgcc
     2701 caaggctgtt tctgcacgcc gtgccaaggt gtttgacccg ataggcggtt acgaatattt
     2761 cgtcaacacc tacttccccc attatatccg ctcgcctgag aaatccgaac tgcatgcgtt
     2821 tttattcagc cgtctgccgg agattatccg ctcccccaaa ggggaaaatg aggcggtggg
     2881 cgcaccgcgt ggcgagggta aatcgacgca ggttacccag ttgtttacgc tgtggtgtat
     2941 tgtgaccggc caaaaacatt atgccgttat tgtgatggac agcatagacc aggcataccc
     3001 gatgctcgaa gccatcaagg cggaattgga atttaatccg cgcctgaaaa ccgactttcc
     3061 tgaagtctgc gggcaaggcc gtgtatggca ggccggtacg attgtgacgg ccaatgacgt
     3121 taaggtgcaa gtggccggta gcggtaaaaa gctgcgcggt ttgcgtcacg gcccttaccg
     3181 tcctgactta actgttttgg acgatattga gaatgacgaa caagtccgca accccgaaca
     3241 gcgcgacaag ctcaatgcgt ggctgactaa aacggtattg cctttgggcg gggtcggtca
     3301 gaaatacgat gtgatttata tcggcacgat tttgcattac gacagcgtac ttaaccgcac
     3361 tttgaataac ccgttttggc ggggtgttaa gtttaaggcg atgaaacgct ggcccgaccg
     3421 catggatttg tgggacaggt gggaggaact tttccgaaac gacggcgaga cggtggccga
     3481 ggcgttttat caggcaaaca aagacgagat ggagcgcggc gcagtcactt cttgggcggc
     3541 gcgtggcgta ctcgcgctga tgaaaatccg tgcgcgtgac ggccatgcga cgtttgattc
     3601 ggaatatcag aatgatccgg tcagtggcga agatgcgccg tttgccaagt cgatgaagtt
     3661 ttggaacgac ctgccgtccg atttggtgta tttcggtgcg ctcgacccgt cgctgggtaa
     3721 agcgggggcg agccgtgacc cgtcggcaat cgtcatcggc ggctatcagc ggtcaatggg
     3781 caggctttat gtcgtggagg cgcaaatcaa aaaacgtctg cccgacctga ttatcgaaga
     3841 cgttatctgc ctgcaccgtc aatatcgttg caaactgtgg tttgttgaga cggtgcagtt
     3901 tcaggagttt ttaaaagacg agctggtcaa gcgcagcgcg gcgcgtggaa tacctgtccc
     3961 ggcgcgggcg gtcaagccgg tatcggacaa actcttgcgg attgagactt tgcagcctca
     4021 catggcaaac ggtttgattc tgttgaatga gagccaacag acgctgatac agcagttccg
     4081 ccattttcca aaggctgatc atgatgatgg tcctgatgcc gtgcatatgc tctggtcggg
     4141 ggcggtggcc aattgtgtgc cgatagaatg gcaaagccct accgataacg attttggtaa
     4201 cgagataaaa agtaaatgga gccgataatg gcaaaaaagg acaataaaac taaaatccaa
     4261 aagcccgagg ctgcattgca gacggatgcg gctcaaatta cggcgaccgg tcgggttatc
     4321 gccgagcatc cgtccaattt tattacgccg caaaagatgc gcgccctctt cgaggacgca
     4381 gaaagcggcg acatccgcgc ccaacacgag cttttcgcgg acattgagga gcgcgacagc
     4441 gacatcgcgg caaatatggg gacgcgcaaa cgcgcgctgc tgacgctcaa ctggcgcgtc
     4501 gccccgccgc gaaatgcgac gcccgaagaa gaaaagctgt ccgaccaagc ctacgaaatg
     4561 atggacagcc tgcctaccct cgaagacctg attatggatt tgatggacgc ggtagggcac
     4621 ggattttctg cgttggaggt cgagtgggta ttttcagacg gcctttacct gccccgaaac
     4681 tttatccacc gcccgcaaag ctggttcaaa tgggacaaag acgacgggct gctgctgcgt
     4741 acccgcgaaa atccggaagg cgaagcgttg tggccgctgg gctgggtcgt tcatacccaa
     4801 aaatcgcgca gcgtccggca ggcgcgcaac gggcttttcc gctcgctttc ctggctgtat
     4861 atgttcaaac actacgccgt ccacgatttt gccgagtttt tggagctgta cggcatgccc
     4921 atccgtatcg gcaaatacgg cgcgggcgca accaaagagg aaaaaaacac cctgcttcga
     4981 gcggtggcgg aaatcggtca caacgcggca ggcatcatgc cagaaggtat ggaaatcgag
     5041 ctgcacaacg ctgcaagcgg tacgacggca accagcaatc cgtttttgca gatggccgat
     5101 tggtgcgaaa aatcggcggc gcggctgatt ttggggcaaa cgctgaccag cggtgcggac
     5161 ggaaaatcca gcaccaacgc gctgggcaat atccacaacg aggtacgccg cgatttgctg
     5221 gtgtcggacg caaaacaggt ggcgcaaacc atcacaagcc aaatcatcgg accgttcctg
     5281 caaatcaact atccccatgc cgacccaaac cgcgtgccga aatttgaatt tgacacgcgc
     5341 gagccgaaag acatcgcggt ctttgccgac gctatcccga aactggtgga tgtcggcgta
     5401 caaatccccg aaagctgggt gcgcgacaaa ctggtcattc cagatgtgca ggagggtgag
     5461 gctgtgttgg tgcggcaggt accggacaat ccggtaaaca gaactacatt ggcggcttta
     5521 tccgcccaca ccgtaccatc taaggctacg ggcaggcatc aggaaatatt ggacggcgcg
     5581 ttggatgacg cgctggttga gcccgatttc aattctcagc tcaacccgat ggtgcgtcag
     5641 gcggttgccg cacttaatgc ttgcaacagc tacgaggagg cagatgccgc actgaatgcg
     5701 ctttatccga atttggacaa cgcgaaactg cgtacctata tgcagcaggc cttgtttatc
     5761 agcgatattt tgggacaaga ccatgcccgc gcctgatttg ggatttgcct taagtctgcc
     5821 gccaaaaaag gcaatcgagt ggctggaaag taaaaaggtt acggcggaga gctaccgcaa
     5881 tctgacagcc tccgaaattg ccaaagtcta tacgattgcc cgcatgaccg acttggatat
     5941 gctcaacgac atcaaaactt cgatggttga atcggcaaaa agtggacagt cgtttgacga
     6001 ttggcgaaaa ggtatcttga atctgctcag caacaagggc tggctgcatc cgaacgggca
     6061 taacggtaag gatatcatcg acccagccac cggcgaggta ttcggttcgc cgcggaggtt
     6121 ggagacgatt taccgtacca acatgcaaac tgcctacaac gccggtcaat atcaaggata
     6181 tatggcaaat attgatgcac gaccttattg gatgtatgac gcggtaggcg acagccgcac
     6241 ccgtccggcg cattcggcaa tagacgggct ggtgtaccgc tacgacgacc cgttttgggc
     6301 aacgttttac ccgcccaacg gctacaactg ccgctgctcg gtcatcgcgc tgtcggagcg
     6361 ggatgtggaa cgccaggggc ggattgtcgg gcaaagcacg gcggataatc tggtcgagac
     6421 ccataaaatc tataacaaaa aaggcgacag ctacccgacg actgcctaca aggccccgga
     6481 cggcagcctc tacacggctg acaaaggctt cggctacaac gccgggcgaa tgaactaccg
     6541 ccccgattta gacaagtacg accgcgcgct ggcgcatcaa tttgccaaag cggaaatggg
     6601 tggtgcagat tttaaaacca gctttaaaca gcttgaaaaa gagttttatg aagtcaagca
     6661 acgtttggat attgatggca agcccgataa agagcagaaa atcaaaatcc gaaatgcgct
     6721 atcaagacag cttaaatttg ctgcgggtgt attgagcaag gaaacgcaag aattggcagg
     6781 tatgacacga gcgacggtgt ggctgtctga tgatacgttg gttaaacagg tagacagccg
     6841 tgaggggcag aatttcgatg actcctacta tgcttttttg ccggatatgc tgcaaaaccc
     6901 tgaacatgtc atccgcgaca atcgtgaatt gattttcaca gctcgctata aaggctcggc
     6961 attgtgggca gttttaaaat atattaagga ggtggatgag atttatctac agtcgtaccg
     7021 aatcagcaac gacaaagaga ttgccaaatt tatggcgaag aagaaagtat tgaaatagac
     7081 gttgggcaag gctcgaattc acttgcacac gctctcgtac accctaaagg gcaggctgcg
     7141 gtatcgagat tatcaccgct tttccaacgc ctataaggaa tcataccatg attgatgtca
     7201 aaatagacaa tatctttgtc gtcctaaacc aaatcgagcg gcttggcaac gggatcgaaa
     7261 accgctacct gctgatgcgc cgactgtccg aaaccatgca cacggcggtc aagctcaatt
     7321 tccgctacgc aggccgtccg aaatggttgg ggctaaaata ccgcgacggc aagccgcttt
     7381 cggattcggg tcgtctgaaa gacagttttt ccacactgtc agacaacgat acagcccttg
     7441 tcggtacgaa tatcgtctat gccgccatcc acaacttcgg cggtatggcg gggcgcaacc
     7501 gcaaagttcg gattccgcaa cgggaatttt tgacgctgac ggacgacgac aaacaggctt
     7561 tgatggacga tgtgcaggat tatttttcgg gtctgatacc gtgaatttat aaaaccctca
     7621 aaaacgcgct ttttagcgcg tttttttatg cgggtaatac aaacccctgc ccaagatata
     7681 aaaatcaatc ctagacgctt ctaaaaagcc cctgaaaacg attaattgtg tatcgcgcgg
     7741 acaggtttta aaaaaatggc gggagggttt gaagcacgcc tactctttgt tgttttttca
     7801 aataggcaaa atgaccgtat tgagagaggt acacatgtcc aaaaatgcac aaaaaaccct
     7861 acttgccgtg tgcagtttcg aggtgcagcc aaaagacggg cgaatccaac tgctgccata
     7921 tggcgaattt cgcgcagtag acggtcgtcc gactgatgtc cctgcgtggt atctgaccga
     7981 agaaaacggt catgatgtcg cgttgttggc caacagctcg cgcaatcagt tggttgtcga
     8041 ttatgaacac cagacgctct acaaagagaa aaacggacaa cctgcacctg ccgccggttg
     8101 gatgcgttgg ctggagttca cgcctaaagg catgtttgcc gaagtggagt ggacggacaa
     8161 ggcggctgcg gcaattgccg caaaagagta tcgctacatc tctgctgtgt tttcctatga
     8221 cacaaaggga tatgtaagca aaatttttca cgcagcgctg acaaatttcc ccgcgttgga
     8281 cggtatggac gaggtgctgg cggcagcgtc ggcgcaaatt ttaaaaccgg aaacggagca
     8341 aaaccctatg aaagagttgt tacagcaact gttcggtctg cctgatgcgg gcgaagaaga
     8401 actgaaggcg gcattgtccg cgctcgtgga agccaagccg gaaggtgcgg ctttgtccgc
     8461 cgatgtattc gcgcagctgg cggaaaaaga cagccgcatt gcggctttgt cggcgcaaaa
     8521 cggcacgccc gacctgacgc gatacgcgcc ggtgtcgctg gtgcgcgacc tgcaaaacca
     8581 agttgccgcc ctgaccgccg agcgcgaagc cgacaaaggc ggcgaactga ttaccgccgc
     8641 actgtctgct ggcaagctgc tgcccgcaca aaaagaatgg gcggaaggcg tgctgaaaca
     8701 gccgggtggc tttgcctttt tgagcggctt tatcgaaaac gcccagccgg tcgctgcact
     8761 ggcaggctcg caaacgggcg gcaaagcacc cgacgaacgc gtcgccgcac tgactgcgga
     8821 agaggcagcc gcagcaaaaa tgctgggcat gtccggcgaa gaatttgtaa aaatcaaaga
     8881 aagcgaaggt aagtaatgga caaatcagcg attttgaccg caatcacggc agcattccgc
     8941 aaagaatttc aaacgggggc ctgcaaaacc gcctgaaccg tttaaccgcc aaacaggtgc
     9001 agccggtagc gcaggcggtg cgcctgttat ccacgtcatc gctgttgtcg gtggcaacgg
     9061 cattaatcga ggcgcatggc gaagagatga ccgcgcccga tttgattgag gttaaccgcg
     9121 ccatgcgccg ccgtatgcag gccgagattg ccgccttgcg ggcggtgcag acggctgctg
     9181 ccgagtctgg tgggctgacg gccaacgccg tgtataccga ggcttaccaa acggcagaat
     9241 ccctgcgcgc ggcggcaggc cgtctgaatg cgttggttgc ggcggccatc aaccaaaagc
     9301 cgccgctgat tgtgcgccaa gccccaatcg acggtacgat acaccaaatc gcccacgagt
     9361 tttacggcga tatggcccgc gcagcagagc tggtgcggct caatccccat atccaccacc
     9421 ccgcgtttat caagcgcggc actttggtca acagctatgc aaaataattc atacggctat
     9481 gccgtgtcgg tgcgcgtggg cggtaaagag caccgccact gggagcgcta cgacatcgac
     9541 agcgactttt taatccctgc cgacagcttc gattttgtca tcggcaggtt ggggccggag
     9601 gcggccatac ccgatttaag cggagagagc tgcgaggtag tgatagacgg gcaaatcgtg
     9661 atgacgggca tcatcggcag ccagcgccac ggcaaaagca agggcagccg cgagttgagc
     9721 ttgagcgggc gtgatttggc cggttttttg gtggattgct ccgcgccgca gctcaatgta
     9781 aagggcatga cggtattgga tgcagccaaa aagctggccg cgccgtggcc gcagattaaa
     9841 gcggtggtgc ttaaggccga aaacaacccc gctttggaca aaatcgacat cgagccgggc
     9901 gaaaccgtat ggcaggcatt aacccatatt gccaactcgg tcgggctgca tccgtggctg
     9961 gagccggacg gcacgttggt ggtgggcggt gcggattaca gcagcccgcc ggtggcgaca
    10021 ttgtgttgga gccgcaccga cagccgctgc aatatcgagc gcatggacat tgagtgggat
    10081 accgacaacc gcttttccga ggttactttt ttggcgcaat cgcacggccg cagcggcgac
    10141 agcgccaaac acgatttaaa gtgggtgtac aaagacccga cgatgacgct gcaccgccct
    10201 aaaacggtgg tggtgtccga tgccgacaat ttggccgcat tgcaaaagca ggctaaaaag
    10261 cagctggccg actggcggct ggagggattt acactcacga taaccgtggg cggccataaa
    10321 acccgcgacg gcgtattgtg gcaacctggc cagcgtgtgc atgtgatcga cgacgagcac
    10381 ggtatcgatg cggtgttttt tctgatgggg cggcggttta tgctatcccg catggatggc
    10441 acgcaaaccg agctgcggct caaagaggac ggtatttgga cacccgacgc ttaccccaaa
    10501 aaggccgagg cggcgcgcaa gcgcaaaggc aaacgcaaag gcgtgagcca taagggcaaa
    10561 aaaggcggca aaaaacaagc agaaacggcg gtgtttgaat gagtttgagt aaattggcga
    10621 aaaaaacggc acaaactgct aaaaatatcg gcgaaaccct gcgcgcggcc tttcggggaa
    10681 aaatcacgct ggtggtgtcg tccgagccga tacagcgcgt gcagttgagc ggcttggccg
    10741 acgaaaccct gcaagacctt gaacatttgc aggaatacgg ctttgccagc catccgcccg
    10801 acggcagcga agcggtagtg ataccgctgg gcggcaatac ttcgcacggt gtgattgtgt
    10861 gcagccagca cggcagctac cgcatcaaaa accttaagcc cggcgagacg gcgattttta
    10921 atcatgaggg tgcaaaaatc gtgattaagc aaggcaaaat cattgaggcc gattgcgacg
    10981 tgtaccgggt taactgcaaa caatacgagg ttaatgcggc cacggatgcc aaatttaacg
    11041 ctccgttggt ggagaccagt gcagtgttga cggcgcaagg ccaaatcaac ggcaacggcg
    11101 gcatggccgt cgagggcggc gacggagcca cctttagcgg cgatgttaac caaacgggcg
    11161 gcagctttaa caccgacggc gacgtggtgg ccggcaatat atcgttgcgc cagcacccgc
    11221 ataccgacag catcggcggc aaaaccttac cggcggaacc ggcatagaca agcagacctt
    11281 tggcagcctt cgggctgctt tttttgtgcg tgtgggattg aagcccgtgt actccgtgag
    11341 gccgtctgaa aacggcaaaa tgccaacatg gacaaagagc taaaccccag catcggcgac
    11401 tataccggcc gcaccgtcga tacgctgcaa aatgccgtgt atatccgctt gatgacaccg
    11461 ttgggcagct ggtgggcgga taaaacgctc ggctcgctgc tgcatttgtt gcagcgcgaa
    11521 aaagacctgc aacgggtcag cctgttggcc gagcaatatg ccgatgaggc actgcaaccg
    11581 attgttaaga gcgggcgtgc cgacaagatt accgtgcgcg cagagcagcc gcacgacggc
    11641 cgcctgatcc tgcatatccg ggtggatacg gcggcgggcg ggtttgatta ccgccacgaa
    11701 gtgcccgtga tttaaagagg ttttaaacgt gtttgaaacg ccgacatttg agcaaatccg
    11761 cgagcgtatc ctgcgcgata ccaaaagcct gtggccggat gccgatatca gccccgacag
    11821 cgaccattat gtgcacgcca gccgtttggc cagctgcgcc gaagggcaat atgcgcatca
    11881 aagctggatt gtgcggcaga ttttccctga taccgccgac cgcgagtatt tggagcggca
    11941 tgcctccatg cgcggcttgc gccgccgcaa tcctaccacg gccagcggca cgctgaccgt
    12001 aagcggtatt gcgcaatcca tgctttcaga cggcctgcaa gtgcgtatcg gccagcgttt
    12061 ttaccgcact accgcccgcg ccgttatcgg cagcggcggc acggcggaaa taccggcaat
    12121 cgccgacgag ctgggcgcgg ccgccaatgt gcgcgacggc gaggcgcaac tgatggccgc
    12181 ccccgccggt gtgtccaccg aatgccgcct taccgtacaa ggcggcaccg accgagaaag
    12241 cgatgcctca ctgctggcgc gtctgttgga aatcatccgc cgaccgcccg caggcggcaa
    12301 ccgttacgac tataaaaact gggcgttgag tgttgacggc gtaaccagcg catatgttta
    12361 tccgctgcgc cgcggcttgg gtacggtgga tattgccatt acctccgccg acggtgtgcc
    12421 atcggaagaa actgtgcgcc gcgtacaggc ttatatcgac gagatgcgcc cggtaacggc
    12481 aaaaaatgcg ctggtactca agccaaccgt aacggcggtg cctgttaccg tgcaagtcaa
    12541 gctcgacggc atcgacttgg acgaggccaa gcgccgcata cggacggccc taaaagaata
    12601 tttcgacacc ctgatccccg gcgacggcct gactgtgtcg caaatcgagg cggctatcag
    12661 caatgtggat ggtgtgatcg accgccgtct gactgcgccg acggccaacc gtgccgccga
    12721 tacggttaac cgcatcgagt ggtttaaagc gggcgcgatt aatgtaacgg agatgccgtc
    12781 atgagctatc aagacatctt gcggggcctg ttgccccccg tgtcgtatgc ccgcaatgcc
    12841 ccgcgtgtgc gggcgcaggc agaaatagac ggcgcagcgc tggatgcggt ggcggaatcg
    12901 gctcaaagcg ttgccgatgc cgtcgacccg cgcagcgccg gccaaatgct ggccgattgg
    12961 gagcgcgtat taggtttgga cggtacgggc aaaaaccgcc agcgccgtgt gttggccgtc
    13021 atggccaagc taaacgaaac aggcggcttg agtattcctt attttgtgcg tttggccgag
    13081 gcggcgggct atcaaatcca aatcgacgaa ccgcagccgt tccgcgccgg tgtaaaccgc
    13141 gccggcgacc gtcttgcgcc gcaggaaatc atgtgggtgt ggcacgttaa cgtgcgcggc
    13201 ggcaacaacc gcattacccg attccgcgcc ggtatctcgg cggcgggcga caggctgacc
    13261 gattacagcg atgccgtgat cgagagcctg ttcaaccgcc tcaagcccgc ccacaccgct
    13321 atccgattta cctaccgata aggactaaac catgcacccc atcgaaaccc ccgacaagac
    13381 cttccacgac ggcgacggcg tgtccgaatt gggcaccatc ctgcccgcgt ggtggctcaa
    13441 ccaagtgcaa tccgagctgc tggccgtgct gactgcggcc ggtatccagc cggataagtc
    13501 gcagcctaat caattactgg cagcactaaa taggctggct gtagttacca ccggcgacca
    13561 aaccatcacc ggccaaaaaa cctttaccgc ccaaacccaa ttccaaagcg gcatccattt
    13621 atccgccaac cagacgaact ggaacggcgg ccacaaagcc tacatcggcg cggatgccga
    13681 caacgcccac atcgtcttcg gcgacgacac cctccgcctg cacggcgcaa acaaccgcat
    13741 ttcctacaac aaccacgaca tcttccacaa agccaacaaa ccgcgttttg ccgaagacat
    13801 cgaaggtaag ccgaacacac tgtccggata cggcatcggc aatttcaaag tcgaaacctt
    13861 ccggggcgat ttgaacaccc tcaaaacaga cggcatctat tccctgccga cggcgggcgg
    13921 cagctccaac ctgcccgttg aaaacaccgc ctgccatatc caagtcatcg ccggcacgaa
    13981 acacggctgg tgcaggcagt tgggttatcc cgcctacacg tccgacgtgt acgaacgcca
    14041 ccaaacgagc agcgcaaacg acaactggtc cgcatggaaa aaactcaatt cggacggcat
    14101 ccccgtcggc gcgatcgtat cctttcccaa agccgtccga aaccccgcag gctatctcaa
    14161 agccaacggc acgacctttg cacaaaacac cttccccgac ctctaccgcg ccctgggcaa
    14221 cagcaaccgc ctgcccgatt taagccgtac cgacatcggc atcaccgcat ggtttccgtc
    14281 cgaccaaatc ccgaccggct ggctggcgtt tgacgacatc cgcacgcgcg taaccgaaac
    14341 cgcttatccc gagctgtacc gtctgctgac cggaaaatac ggcagcatcc aaaacgtccc
    14401 gcaggcggaa gaccgcttta tccgcaacgc cgccaacggc ttggcagtcg gaacgaagca
    14461 ggaagacgaa atcaaacggc acgtccacaa agtattttca cactgggtaa accacccgca
    14521 cgcggcagcc ctcggttacg aagaccacaa cgaaaggcag agaagcgcgc tcgtatcgac
    14581 ttggacggac gaaaatttaa acgacaacgg ctttttaacc ccgcgcccgg acagcaaaat
    14641 ggcgacaggc ggcgacgaaa accgccccaa agccctggtt ttaaaactgt gcatcaaagc
    14701 cgccgacacc ttgggcgaag ccgtgttctg gataaagtcc cacggcgaaa ccgtcaacgc
    14761 cggcgcgctg gacgcgggca cgctggcgca aaacctccaa gacaaagcag accgcgccca
    14821 cacccacacc gccgcccaaa tccaagggct ggacgaaaaa atcagcaccg ccgttgccgc
    14881 gcaattcaca cgccaaacca tcggcggcgt ggatattgtc agattccccg acggcacaat
    14941 gatacagacc ggcagctaca ggttcacacg aagcggcggc cccatagaaa acgaagtcgt
    15001 cttccccgtc gcctttgccg acggcaacgt caaatgcttc gtatccgaac gccattcggg
    15061 acgcgttacc ggcgatcgaa ggcaacacaa ctggctgttt atccgcgcaa aaaaccacgc
    15121 cgccgccatt attaccaact ggtacgaagg cagttgcgac tggatggcca tcggcaaagc
    15181 cgcctcggga aacgccgcca gctccccgat aggccccgaa atacctgaaa ccaacgaaga
    15241 accgcaaaga gagagtggaa gaacatcaac cggcccccga aaccgccgcc accgagacag
    15301 cttgctcgag gcactgcaag actag
//
LOCUS       DAXHMX010000036        15207 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHMX010000036 DAXHMX010000000
VERSION     DAXHMX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.036"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            275..1429
                     /gene="obgE"
                     /locus_tag="ACFM4S_000817"
     CDS             275..1429
                     /gene="obgE"
                     /locus_tag="ACFM4S_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6050539.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1512..2561
                     /locus_tag="ACFM4S_000818"
     CDS             1512..2561
                     /locus_tag="ACFM4S_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6050540.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3249..4670
                     /gene="cysS"
                     /locus_tag="ACFM4S_000819"
     CDS             3249..4670
                     /gene="cysS"
                     /locus_tag="ACFM4S_000819"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6050541.1"
                     /translation="MTTIYNTLTRQKELFAPIDPKNVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4841..5620
                     /locus_tag="ACFM4S_000820"
     CDS             4841..5620
                     /locus_tag="ACFM4S_000820"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6050542.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5607..5951
                     /locus_tag="ACFM4S_000821"
     CDS             5607..5951
                     /locus_tag="ACFM4S_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050543.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVRTLLSVPAT
                     LLIAFTLQDLLK"
     gene            5961..6491
                     /locus_tag="ACFM4S_000822"
     CDS             5961..6491
                     /locus_tag="ACFM4S_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6050544.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQVDILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6584..7699)
                     /gene="asd"
                     /locus_tag="ACFM4S_000823"
     CDS             complement(6584..7699)
                     /gene="asd"
                     /locus_tag="ACFM4S_000823"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6050545.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQPLRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAKVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPVSEIEAILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLEMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7875..8456
                     /locus_tag="ACFM4S_000824"
     CDS             7875..8456
                     /locus_tag="ACFM4S_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6050546.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8518..9372)
                     /gene="folD"
                     /locus_tag="ACFM4S_000825"
     CDS             complement(8518..9372)
                     /gene="folD"
                     /locus_tag="ACFM4S_000825"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6050547.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9595..9672
                     /locus_tag="ACFM4S_000826"
     tRNA            9595..9672
                     /locus_tag="ACFM4S_000826"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9630..9632,aa:Pro,seq:tgg)
     gene            9697..9773
                     /locus_tag="ACFM4S_000827"
     tRNA            9697..9773
                     /locus_tag="ACFM4S_000827"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9731..9733,aa:Arg,seq:tct)
     gene            9801..9876
                     /locus_tag="ACFM4S_000828"
     tRNA            9801..9876
                     /locus_tag="ACFM4S_000828"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9834..9836,aa:His,seq:gtg)
     gene            10012..10518
                     /gene="rfaE2"
                     /locus_tag="ACFM4S_000829"
     CDS             10012..10518
                     /gene="rfaE2"
                     /locus_tag="ACFM4S_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC6050548.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGDALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10515..12293
                     /locus_tag="ACFM4S_000830"
     CDS             10515..12293
                     /locus_tag="ACFM4S_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6050549.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12406..13230
                     /locus_tag="ACFM4S_000831"
     CDS             12406..13230
                     /locus_tag="ACFM4S_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6050550.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(13993..14781)
                     /locus_tag="ACFM4S_000832"
     CDS             complement(13993..14781)
                     /locus_tag="ACFM4S_000832"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6050551.1"
                     /translation="MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     AGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(14995..15189)
                     /gene="thiS"
                     /locus_tag="ACFM4S_000833"
     CDS             complement(14995..15189)
                     /gene="thiS"
                     /locus_tag="ACFM4S_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC6050552.1"
                     /translation="MNIILNGGPAELHGTTVADLIAQTAPQKPFAVAVNTGFVAKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3636 a         3856 c         4214 g         3501 t
ORIGIN      
        1 aaacttatta tatgtaacgg tttgaacgga cattccgtcg ggtcggaatg tcaaaaaggc
       61 gcgattgtac caaagaagtt ggacataatt ttgtttgcag gctgaagatt tgcttaaaaa
      121 ttcattaaga tgggcggaac aaatagtttg ggtacggcgt gttgaaatac cgcttatccc
      181 ttataatagc gtccgaaatt ctgttcggac ggcatcaaaa cacacggtaa tccgtctgaa
      241 ccccccattt gaaaccaaca aacaagagca ttgaatgaaa ttcatcgacg aagcaaaaat
      301 cgaagtcgcc gcaggcaaag gcggtaatgg cgcaaccagt ttccgccgcg aaaaattcgt
      361 accgcgcggc ggcccggacg gcggcgacgg cggcaaaggc ggcagcgtct gggcagaagc
      421 cgacgaaaac accaacaccc tcgtcgaata ccgcttcgtt aaacgctacc aagccaaaaa
      481 cggcgaaaaa ggccacggtt ccgaccgtta cggcgcaggt gcggacgaca tcgtcctcaa
      541 aatgcccgtc ggcaccctca tccgcgacct cgacaccgac gaaatcgttg ccgacctcac
      601 ttaccacgga cagcgcgtct gcctagccaa aggcggcaaa ggcggcttgg gcaacatcca
      661 cttcaaatcc tccgtcaacc gcgccccaaa acaatccacg cccggcgaag aaggcgaagc
      721 ccgttccctg caactcgaac tcaaagtcct cgccgatgtc ggcttattgg gtatgcccaa
      781 cgccggcaaa tccaccctga ttaccgccgt atccgccgca cgccccaaaa tcgccaacta
      841 ccccttcacc accctgcatc caaacttagg cgtggtacgc atcgacgaaa accacagctt
      901 cgtgatggcc gacatccccg gcctgattga aggcgcggca gaaggcgcgg gtttggggca
      961 tcgtttcctc aaacacttat cgcgcaccgg attattgctg cacgtcgtcg atttggcgcc
     1021 cttcgacgaa accgtcaacc ctgccgaaga agcacttgct atcatcaacg aattgcgcaa
     1081 atacgatgaa gaactttacg gcaaaccgcg ctggctggtg ctgaataaac tcgacatgct
     1141 tgacgaagaa gaagcccaaa cgcgcactgc cgccttcctc gaagccgtcg gctgggacta
     1201 ccccaaaccc gacgaccgtt tccaatttga catggaaacc ccgcgcctct tccaaatcag
     1261 tgcgctgacc caccaaggca cgcaggaatt ggtacaccaa atcaaccaat acctgaccga
     1321 gaaaaaacgt atcgaagctg aaaaagccga agcggagaaa gcagcggcaa atgttgaaat
     1381 tatcgaacag cagcctaaaa cggatacagg cgtgtttaag ccggagtgaa aaaagaaagg
     1441 gtttcagacg atatttgttc tacaaaaggt tgtttgaaac aattttaaag caagattgaa
     1501 atggattaaa aatgtcatct aaccaatata tccagtcgct tgagttgaca aactttaccg
     1561 ttcttccaaa cgatactttt gaattttctg aaaatctcaa tgttattgtt gccgaaaacg
     1621 gttgtggcaa aacacatttg ttaaaaattt tatacagcct gctagaagta acatctaaca
     1681 ccaaaaacag attactgaaa acagaattgc aaaaaagttt tgccgataaa ttactgaatg
     1741 ttttccgtcc cgattccttg ggcaggttga gcaaacgttt gcaggggcgc gggcgtacgg
     1801 aaatcgtttt aaaactgcaa aacggcacta cgcatagtag tctgaatttt tccagtaact
     1861 catcatcgca agtcaatgtt caaagcatcg gattgaaaga aaatgaatat actccgacac
     1921 ctatcttctt acccagccgc gaattaatta cactttgccc ttggttcact tctttgtatc
     1981 aaaaccaaag catccctttc gaagaaactt ggtttgatac ctgtatgcaa ttaaatcacc
     2041 cgcttgccaa aggccccaga gaaacaaaaa ttcgagaatt gctcgaacct attgagaacg
     2101 cgatgggcgg aaaagtatct gaagaacaag gaaggtttta tctatcgctg acaaatacgg
     2161 gcggtaagat tgaagcacct ttggtagcag agggtttgcg gaaatttgtc atgattgccc
     2221 ggttaatcgc aacaggggca ttgctggata aaggctatct attttgggat gaaccggaag
     2281 ctaatctgaa tcctaaattg atcaaagtgg ctgcccgtat tatttggtct ttatctcaac
     2341 agggtattca ggtttttatt gcaacacact ctttattctt attgagagaa ttggaacttt
     2401 tgaagcttga aaataacacg gatttacctg tacgtttttt ctcgttgatt gcttccgatg
     2461 atggaacaaa agtggagcaa gggaacagta ttaatgattt gaacacattg atattgttag
     2521 atgaaaacat tatgcaggca gacaggtatc tgaatatttg aggttaagct atgactacaa
     2581 tcacagaaaa gagattaacc tttgcttttc ctgaagatta ttacgttacc aagtatgacg
     2641 aatgggaaca ttataaaatt tttcagaaca gttgtaattt acgtaataaa attgatacaa
     2701 atgaaaaggg aaagaacggg ataaatcaaa gtgtagatga cgataatggc agctcaggtg
     2761 tagatataat agcccttcat gagtcgacac tttggctaat cgaaattaaa gattattacc
     2821 gacttgggct tgagccaaat gcccaatcaa tagatgaaaa actttctgac ctaccatatt
     2881 taatcgcaag aaaaattcga gattcattgg caggattggt ttctgctaaa tttaaagcag
     2941 aaaaacagga agaaaaagat ttttctcgct tggctttaga ttgtaacgaa ataaaaatag
     3001 tccttcatat tgaaatgcct agtataagaa gtaaacttta tccctcgtca tcagatttag
     3061 caaacttatt aaaagataag tttaaattat cagaatttac aaaaaatttt gcaaactgtt
     3121 atgcagaacc aattttcaca aatatcagcc atattaataa tccgcaactg cgtaatgttc
     3181 cttggtccgt atctacagga acagaacaga aattatcatc cgagcaacaa agattaatcc
     3241 ataatcccat gaccaccatc tacaacaccc ttacccgtca aaaagaactc ttcgccccca
     3301 tcgatccgaa aaacgtgcgt atgtacgttt gcggcatgac cgtttacgac tattgccact
     3361 taggccatgc ccgtgtgatg gtggtgtttg acatgattgc ccgctggttg cgcgagtgcg
     3421 gctatccgct cacttatgta cgcaacatca ccgacatcga cgataaaatc attgcccgtg
     3481 cggctgaaaa cggcgagact atcggcgaac tgaccgcgcg tttcattcag gctatgcacg
     3541 aagatgccga tgctttgggc gtgttgcgtc cggatattga gccgaaggca acggaaaaca
     3601 ttccgcaaat gattgccatg attgaaaccc tgattcaaaa cggcaaggca tatcctgccg
     3661 caaatggcga cgtttactac gccgtgcgcg agtttgccgc ttacggacaa ctgtcgggca
     3721 aatcgttgga cgacctgcgc gcgggcgaac gcgtggaagt ggacggtttc aaacgcgatc
     3781 cgcttgattt tgtattgtgg aaagcggcga aagcaggcga accggcgtgg gaaagcccgt
     3841 ggggcaacgg ccgtccgggt tggcacattg aatgctctgc catgagtgaa aacctgttcg
     3901 gcgatacctt cgacatccac ggcggcggcg cggatttgca gttccctcat catgaaaatg
     3961 aaatcgccca aagcgtcggc gcgacggggc atacctgcgg tcatcatcac gcgcaaaccc
     4021 accacgggca aagcatcgcc agccacgtca aatattggct gcacaacggc tttatccgcg
     4081 tggacggcga aaaaatgtcc aaatcactgg gcaacttctt caccatccgc gaagtgttga
     4141 aacaatacga ccccgaagtc gtgcgcttct tcatcctgcg cgcccactac cgcagcccgt
     4201 tgaactattc cgacgcgcat ttggacgatg caaaaggtgc gctgacccgt ctgtacacca
     4261 ccttgaaaaa cacgccggcg gctgagtttg atttatccga aaacgccaac ggctacaccc
     4321 gccgcttcta cgccgccatg aacgacgatt tcggtacggt cgaagcggtt gccgtgttgt
     4381 tcgaactggc aggcgaagtg aacaaaacca atgacgcaca gctcgccggc tgcctgaaag
     4441 ccttgggcgg catcatcggc ctgctgcaac gcgacccgac cgagttcctg caaggcagcg
     4501 cggcttcaga cggcctctcc aacgaagaaa tcgaagactt gatcgcccgg cggaaacagg
     4561 cgcgcgccga taaaaactgg gcggaatccg accgcatccg cgaccttctg aacgaacaca
     4621 aaatcatttt ggaagacaac gccggcggca cgacttggcg gcgcggttga actgtatcgg
     4681 aaagggcaaa agtaccgcct tttgctcttt ttccgttttc agacggcatt tcccgcttga
     4741 agccccgccg ttttcagcag gcagggttga atgggaaatg ccgggcggcg caccaggtgc
     4801 cgttttgtgt atgggaacat tgtgagaact tatttttatt atgcttaaaa tcatttccgc
     4861 caacgtcaac ggcatccgct ccgcctacaa aaaaggtttt tacgaataca tcgccgcatc
     4921 gggcgcggac attgtctgcg tgcaggaact caaagcgcag gaggctgatt tgtctgccga
     4981 tatgaaaaat ccgcacggga tgcacggcca ttggcattgt gccgagaagc gcggttacag
     5041 cggcgtggcg gtgtacagca aacgcaaacc cgacaatgtg caaatcggta tgggcattga
     5101 agaattcgac cgcgaagggc ggtttgtgcg ttgcgatttc ggcaggttga gcgttatttc
     5161 gctttacttg cccagcggca gcagcgcgga agaacgccag caggtgaaat accgtttcct
     5221 cgatgcgttt taccctatgc tcgaagcgat gaaaaacgaa gggcgcgaca ttgtcgtttg
     5281 cggtgactgg aacatcgccc accaaaacat cgacctgaaa aactggaaag gcaaccagaa
     5341 aaattcaggt ttcctgcctg aagagcgcga gtggataggc aaggttatcc acaagctcgg
     5401 ttggacggat atgtggcgca cgctttatcc cgatgtgccg ggctacactt ggtggagcaa
     5461 ccgagggcag gcgtatgcga aagatgtcgg gtggcgcatc gattatcaga tggttacgcc
     5521 cgaacttgcc gccaaagccg tgtccgcaca cgtttataaa gatgaaaaat tttccgacca
     5581 tgcgccgctg gtcgtggagt atgactatgc tgccgaataa agttttgggt aaatatgatt
     5641 ggaatgtgga cggaaaaaca ggaataggcg cggcatgggt tgtcgctgcg tttatcctgc
     5701 ccatattggt gtgggcgata tttatgttgt cgcggatgca aggctggctt gcgccgacca
     5761 aggcaaaccc gatatgggcg ttggtgtggc tgctgatttg cctgccctgc ctgctgattg
     5821 cgtccaaatg tttgggttgg aaaggctggc ggctgttggc aaacgttctt gcctgcctcg
     5881 ccgtgcgcac cctgttgagt gtgcctgcca cgctgctgat tgcgttcacg ctgcaagacc
     5941 tgctcaaata ggcgcggaca atggaaagtt tcgagcttga ggatttgacg ctgtggctgg
     6001 tacgcgatgc ggatgaagcg gaaatgtgga tagaccgctg ggcgatcagt tatcccgtcg
     6061 tgcagatgtc cgaagcgtcg gccggtcaaa gcatagggga atggcaggca gggcttcaga
     6121 cggcatttga acgcatacgc ggcaaatacg tcgccgttgt cgcacacggt gcgggcgcgg
     6181 ccgcattttt ggcgtggctg tatcaggtag acatcctgac acggaagaaa attgccaaca
     6241 tcatccttgt accgcagcgt cccgatattt ttcccgacga tgcggaacac actttccaac
     6301 gcgtccgctg tccctgccgt gccgcattgg ttgtacccga acacggcggc gtgccgcacg
     6361 gttgggcgca aaaacaggcg gatttgtgga acgcccgcct gttggtttcc ccgcattcgg
     6421 gcagtttgaa cggtatgctc ggcggctggc agtggggtat gaagctgatg caggaaatgt
     6481 tgctggcgtg aacgcttgcc ttatcggaat ttcggttaac tggggttgca aggaaaatgc
     6541 cgtctgaaac aggcttcaga cggcatttcc attcaaacag ggcttacagg ctgcccaaca
     6601 cgatacgcag cacgcggcgc aacggctcgg cagcccccca caagagttgg tcgccgacag
     6661 tgaacgcgct gatgtattcg ccgcccatct ccagtttgcg gatacggccg acaggaacag
     6721 acagtgtgcc ggtaactttg gcaggagtca gctcgtgtat gctggcttct ttttcgttag
     6781 ggatgacttt cacccagtcg tttgcacctg ccaaaatcgc ttcgatttcg gaaacaggca
     6841 ggtctttttt cagcttcagg gtgagggctt ggctgtggca gcgcatagag ccgatgcgta
     6901 cgcataagcc gtcgattacg gtcggattgt cgctgcggcc gaggattttg ttggtttcca
     6961 caccgccttt ccattcttct ttagattgac cgttgcccaa atccacgtca atccacggaa
     7021 tcaggctgcc ggcgagcggt acgccgaagt tggctttcgg atagtcttcg ctgcgcagga
     7081 aatcggacac tttgcggtcg atgtcgagaa tcgcgctgga aggatcggca agctcgtccg
     7141 ctactttggc gtgaatcgcg cccatgccgc tgatgagttc gcgcatgttt ttcgcacccg
     7201 caccggaagc ggcttggtag gtcatgctgg ttgcccattc gaccaaatcg ttttggaaca
     7261 gaccgcccaa agccatcagc atcagggaaa cggtacagtt gccgccgatg tagtttttca
     7321 cgccgttttt gaggccgttg tcgatgacgt tgcggttgac cgggtcgagg acgataatcg
     7381 catcgtcttt catgcgcagg gatgacgccg cgtcaaccca gtagccgttc cagccgctgt
     7441 cgcgcagggg ttggaagacg gatttggtgt aatcgccgcc ctgacaggta acgatgatgt
     7501 ccatttttgc caattcggca acatcgttgg catccaacaa tgttttggct gcctgaccga
     7561 aatcaggggc tgcgccgccg acgttggaag tggtaaagaa aaacgcttca ggaatgtggg
     7621 cgaagtcgtt ttcttctttc atacgctgca tcaaaaccga accgaccata ccgcgccagc
     7681 cgacgaaacc tactttcatt gtgttgctcc ttgaggaaaa atcgttaata aaattatgta
     7741 tggcgtaaag gaagcgtttt aacgccgtat tgggaaaatg taaagttctt tatcgggctt
     7801 tctttaagta ttggcgtttt cggtgtcgtg gcggtagaat cggggtcagt taagaacaat
     7861 tatcaggaac ggatatggaa gaaaaaaacg actacaccga cgcggcatcc gacaaccgca
     7921 acgggcagga aattgaagtc ggaattgccg gcgcaggcga ccggatactt gccgcgctgc
     7981 tgaaccaatt gtttaccttt ttggttttgt tggtgccgtt tgccggactg attgctttcg
     8041 ccatcaagaa tgaaggcagg attggcggca gtgaggaaat attcgggctg cttttgggca
     8101 tgacttcctt ttgggtagga ctggcgggca ttttggttta taccgtcgtt caaatctact
     8161 atatgagtcg ggacgggcag tcattgggta agaagatcat gagaatcagg gtgttgaaaa
     8221 ccgacggccg caatccgggt tttgtcggca cggttttggt acgcgaaatc gcatggtcgg
     8281 ttttggttgc cattattgcc gccgttatcg gtcttgcagt aggtgacaac ggagaaaacg
     8341 ccatcaacct gctggcattc cttgccaact ttgtcctgct ctttatggtc aaacgcgacc
     8401 gccgcacgct ttacgacata ctggcggata cggttgtcgt caagctgccc aaataaacgg
     8461 acacggcaaa atgccgtctg aaagcctttc agacggtatt tttatcttca gaaccgctca
     8521 agcatcgtgc agtgaagccg cgtgcagggt gttttccatc aatgtggcaa tcgtcatcgg
     8581 acccacgccg ccgggaacgg gcgtaatcat cgccgcccgt tcttttgccg tttcaaattc
     8641 cacgtcgccg cacaggctgc catcgtccaa acggttgatg cccacatcaa taacgaccgc
     8701 gccaggtttg atccattcgc ctttgacaaa gttcggaatg cctacgccga ccaccaaaat
     8761 atcggctccg gcaacctcgt ctgtcagatt ttcggttgcg ctgtggcaga ccgttaccgt
     8821 tgcgcgcgcc agcagcagtt ccaaagcctg cgggcggccg acgatattcg acgcgccgac
     8881 cacgaccgct tttttcccct tcggatcaat gccgtaagct tccaaaagcg tcatcacgcc
     8941 cttgggcgta cacgggcgca tcagcggcat tttgaccgcc agcctgccga cattgtaagg
     9001 atggaagccg tccacgtcct tatccggcga aatacgttcc aaaaccgcct ggctgtcgag
     9061 gtgcttcggc agcggtagct gaaccagaat accgtccact tcggaatcgg cattcaggcg
     9121 gtcgaccagt gccagcagtt cttcctgcga tgttgattcg ggcagctcgt aagacagtga
     9181 tttgatgccg catttttggc aggcagtttt cttgttgcgg acataaaccg cgctggcagg
     9241 gtcgcctccg accaaaacca cggccaggca aggcatgtgc agattgtcct gttggcgttg
     9301 cgccaccgct tcggcaaccg cctgcaggcg tttttgcgaa acttctttac cattgatcag
     9361 ttgtgccgac atatccgtcc ttttgttaaa caggtagaaa atgctgaaaa ttatcgcatt
     9421 ttccgtttgt ttttacagca ggatgccgaa aataaatttg ccctgaaata aaatacggct
     9481 gtttggacgg attaaatatt ttttcaaatc aaaaaactat atttttcatc ttggtgtctt
     9541 gaaaataaat attgacgcat ctttccctga gttgtatagt tcgcttcttc aagtcggggc
     9601 gtagcgcagc ccggttagcg catctgcttt gggagcagag ggtcgtgagt tcgaatccca
     9661 ccgccccgac caatcaatca gcacgaatcg gcatgagcgc ccgtagctca accggataga
     9721 gcaccgacct tctaagtcgg gggttacagg ttcgattcct gtcgggcgtg ccaagaattt
     9781 gattgaaaac cgtttttatg gtggctgtag ctcagttggt agagccccgg attgtgattc
     9841 cggttgtcgt gggttcgagc cccatcagcc accccagata aaggcccgta cttttcggta
     9901 cgggtctttt cccgtttgcg cgtactgtcc gcgccgccgg ctttgccttt cgcatatgct
     9961 tctgtatcat gtgggttttt ctgtctttta tggtttttgt cggaggcttt gatggttgac
    10021 gcttggtctg tccctgattt tgaatcgaaa atctgtccgc ccgaggcgtt ggcggcgcgt
    10081 ttggcgttgt tgccgcgccc gctggtgttt accaacggct gtttcgacat cctccacagg
    10141 gggcacgtta cttatctggc gcaggcgcgt tcgatggggg acgcgttggt gctggcgttg
    10201 aataccgatg cttcggtgcg gcgtctgggc aagggcggtg accgcccggt taatcctttg
    10261 gagaaccgtg ccgccgttgc cgccgcgttg gaaagtgtgg atttggtaac gtggtttgac
    10321 ggggatacgc cggcggcgtt gattgaggcg gtcaaacctg agattttggt caagggcggc
    10381 gattgggctg cggataagat tgtcggtgcg gcagaaacgt tggcgcgcgg cggtcaggtg
    10441 ttttcaattc cgtttctgca ccagacttcg acaacgaaga ctttggcaaa aatccgtgcg
    10501 gcagagggcg gaaaatgacg gttttgaagc tttcgcactg gcgggtgttg gcggagcttg
    10561 ccgacggttt gccgcaacac gtctcgcaac tggcgcgtat ggcggatatg aagccgcagc
    10621 agctcaacgg tttttggcag cagatgccgg cgcacatacg cgggctgttg cgccaacacg
    10681 acggctattg gcggctggtg cgcccattgg cggttttcga tgccgaaggt ttgcgcgagc
    10741 tgggggaaag gtcgggtttt cagacggcat tgaagcacga gtgcgcgtcc agcaacgacg
    10801 agatactgga attggcgcgg attgcgccgg acaaggcgca caaaaccata tgtgtgaccc
    10861 acctgcaaag taagggcagg gggcggcagg ggcggaagtg gtcgcaccgt ttgggcgagt
    10921 gtctgatgtt cagttttggc tgggtgtttg accggccgca gtatgagttg ggttcgctgt
    10981 cgcctgttgc ggcagtggcg tgtcggcgcg ccttgtcgcg tttaggtttg gatgtgcaga
    11041 ttaagtggcc caatgatttg gttgtcggac gcgacaaatt gggcggcatt ctgattgaaa
    11101 cggtcaggac gggcggcaaa acggttgccg tggtcggtat cggcatcaat tttgtcctgc
    11161 ccaaggaagt agaaaatgcc gcttccgtgc aatcgctgtt tcagacggca tcgcggcggg
    11221 gcaatgccga tgccgccgtg ctgctggaaa cgctgttggt ggaactggac gcggtgttgt
    11281 tgcaatatgc gcgggacgga tttgcgcctt ttgtggcgga atatcaggct gccaaccgcg
    11341 accacggcaa ggcggtattg ctgttgcgcg acggcgaaac cgtgttcgaa ggcacggtta
    11401 aaggcgtgga cggacaaggc gttttgcact tggaaacggc agagggcaaa cagacggtcg
    11461 tcagcggcga aatcagcctg cggtccgacg acaggccggt ttccgtgccg aagcggcggg
    11521 attcggaacg ttttctgctg ttggacggcg gcaacagccg gctcaagtgg gcgtgggtgg
    11581 aaaacggcac gttcgcaacc gtcggtagcg cgccgtaccg cgatttgtcg cctttgggcg
    11641 cggagtgggc ggaaaaggcg gatggaaatg tccgcatcgt cggttgcgcc gtgtgcggag
    11701 aattcaaaaa ggcacaagtg caggaacagc tcgcccgaaa aatcgagtgg ctgccgtctt
    11761 ccgcacaggc tttgggcata cgcaaccact accgccaccc cgaagaacac ggttccgacc
    11821 gctggttcaa cgccttgggc agccgccgct tcagccgcaa cgcctgcgtc gtcgtcagtt
    11881 gcggcacggc ggtaacggtt gacgcgctca ccgatgacgg acattatctc gggggaacca
    11941 tcatgcccgg tttccacctg atgaaagaat cgctcgccgt ccgaaccgcc aacctcaacc
    12001 ggcacgccgg taagcgttat cctttcccga ccacaacggg caatgccgtc gccagcggca
    12061 tgatggatgc ggtttgcggc tcggttatga tgatgcacgg gcgtttgaaa gaaaaaaccg
    12121 gggcgggcaa gcctgtcgat gtcatcatta ccggcggcgg cgcggcaaaa gttgccgaag
    12181 ccctgccgcc tgcatttttg gcggaaaata ccgtgcgcgt ggcggacaac ctcgtcattc
    12241 acgggctgct gaacctgatt gccgccgaag gcggggaatc ggaacatact taaaaaatac
    12301 accctttttc aacacggggc ggcatccgtc cgaatccgtc cggattctgc cgcccgacag
    12361 actgccgccc aggcgggcgg ggtttcaaac attgaggaaa ttctaatgaa atggctcttc
    12421 acccttttgg cggtactcaa tattgccgta ttcggcggca cggtgggcta taaattggcc
    12481 gttaaagcga cgggcggcgt gccggaaaac cgggccgtcg aaaacacgcc ccccgcaacg
    12541 cccgccgccg acaatgcggc ggcatccgtg gaggatacgg cggcactgct caaaccgggc
    12601 gacatcctga ccgaagagca ggccgagcag ttgcgcttga aaaaggaagc ggaacagaaa
    12661 aaactgaggg agaaaaaaca gcgtgaagaa aaagcccgcc gcgaaaaact cgccgcagaa
    12721 aaggcgcagg cggaacgcga aaacggcgcg gcggatgcct tatgcgccgc gcaggcaagc
    12781 ctcacgatgg acgaagatga ctaccaccgc atcaaaggac ttttgggcaa atggtcgcac
    12841 gttgccagca ggagcgtcga aaaacgcacc gccaaagcca aacctgccga caaaacctac
    12901 cgcgtcgtcc tgcccgtttc cgccgatgcc gaaaatcagg cggcggagct gtctgccaaa
    12961 ggtttcaacc ccataccgtt tgacggcgca ttgagtttgg gtgtcggcaa cagccgggaa
    13021 aacgcccaag ccctgcaaaa ccggcttgcc ggcgccggat tcggcggggc gcatattgtc
    13081 gaacactttg ccgaagccga caggcaggac gattctttga gcgtgtcgcg tatgacggtt
    13141 ttgtttaccg gcgtgaatgc cgccgatgcg gacgaaattc gtaaaatcac gtccctatac
    13201 ggcaaactga acctcaagtc ttgcaaatag gcgcgaaagc cggacgataa acgccaaacc
    13261 caccgcgaag gtgggtttgg cgtttcggtt ttgagtatct gatatataaa aagggaatgt
    13321 atgtctcgta tatgccgact ccaagtgtga aagtgatgat ggggaaatat ggtgtttaat
    13381 ggttcggaac ggacgacttc cgagtggttt tgattatacg ctaataataa taattatcaa
    13441 taaaaattta aaaataaatg cagatttttt gtaatacgat gaaaaataaa caaattaaac
    13501 tatttgccga tgttggtgat tcgggtcggc aaactttcat tgtgtcggac aaatcggggt
    13561 cggcagtcat agctgagggc ttcgtgcctg acggaaataa ggttgcccgt gtttgaacat
    13621 ccgtccgatt gggcgcggac gaggttgtca ttcgggggtg tttgagcgtg gattcgggga
    13681 tgcaaaagcg gtaaaaccgt tgcggatgaa ggcttacgga cggctttagg tatttttaag
    13741 tgtattcaaa caacaacaaa acgcttcttc tattgccgta tttagggctt gcgcggcagc
    13801 ctgcacaccg tggaggccga gtgtttcgtt ggttgcggca tcgggacggg cggcaaggtt
    13861 ttccggccgg tttttccatt ttttccgaca aagccgtgat gccggggaat ggcggtttgt
    13921 ccgtttcgga ccgccgccgt tttgagccgc gatgccgtct gaagccttca gacggcattt
    13981 ttgccgcctt tttcaatatt ccgccgaatg ccaaaacggc tgtccgactg tcggcgtgct
    14041 ggcttgcgct ttgtcgcgtg cttcgaccgg tccggcttca aatgccagcc gtccggattc
    14101 gacggcgagt gcgaaggcgc gtgccatatt gactggatcg ccgctgcggg aaacggcggt
    14161 gttcaaaagc acgccgtcaa agccccattc catcacttgt gccgcctgtg agggcaaacc
    14221 caagcccgcg tcgataatca gcggcgtgta gggcaggcgt tcgcgcagga cgttcaacgc
    14281 gtaggcgtga accgcgccca aacccgtgcc gatcggggcc gcccacggca tcaacgcctg
    14341 acagcccgcg tcgagcaggc ggcggcaggc aatcaggtct tcggtgcaat aaggcagcac
    14401 tttgaagccg tctttaatca ggatttccgc cgcttcgaca agctggaaca catccggctg
    14461 caaggtgtcg tcatctccga tgagttccaa ttttatccaa tcggtttcaa acacttcgcg
    14521 cgccatttgc gccgtcgtta ccgcttcctg cacgctttgg cagcctgccg tgttcggcag
    14581 gacgggaacg ccggtttctt gaagcagcga ccaaaacccc tgaccgtgcg cctcgccgcc
    14641 gcatcccgcg cggcgcagcg agacggtaat catcgcgggc cgggcggttc ggacggattg
    14701 tttgaggatt tcaggggtcg ggtaggcggc tgtgccgagc agcagccgcg aagggaaagt
    14761 ttcgctgtac agggtgagca taatgggttc ctttgtatag tggattaaca aaaaccagta
    14821 cggcgttgcc tcgtcttagc tcaaagaaaa cgattctcta aggtgctgaa gcactaagtg
    14881 aatcggttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttaaagtt
    14941 aatccaccat aacgttgttt tggggacagg ggtcgtctga aaaggcaaaa ccgcctagcc
    15001 gccgaccacc ggccgcacga tatcgatttt gtcgttttcg tttaaaacgg tttccgcata
    15061 cgcgcctttg gcgacaaatc cggtattcac cgctacggca aaaggctttt gcggcgcggt
    15121 ttgggcgatg aggtcggcaa cggtcgtgcc gtgaagttcg gcgggtccgc cgtttaagat
    15181 gatgttcatg gtttctcgat gtttctg
//
LOCUS       DAXHMX010000037        14937 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHMX010000037 DAXHMX010000000
VERSION     DAXHMX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14937
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.037"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            40..1143
                     /gene="dnaN"
                     /locus_tag="ACFM4S_000834"
     CDS             40..1143
                     /gene="dnaN"
                     /locus_tag="ACFM4S_000834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6050553.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1112..1381)
                     /locus_tag="ACFM4S_000835"
     CDS             complement(1112..1381)
                     /locus_tag="ACFM4S_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050554.1"
                     /translation="MPSETFPFQTAWFYSNLNRADWNQKHTFAIPAQALPLYAPNPNP
                     PHSRRHCLSDSRTKPRIRLHGSGHISNTGIVLRKTVKSASASQYI"
     gene            complement(1406..3463)
                     /gene="ppk1"
                     /locus_tag="ACFM4S_000836"
     CDS             complement(1406..3463)
                     /gene="ppk1"
                     /locus_tag="ACFM4S_000836"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6050555.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLNVDEEDVQNLRAAVQNELHDREYGDGVRLEVADTCPARIRD
                     FLLSQFRLTADELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPAVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKIHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIRQETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(3632..4075)
                     /locus_tag="ACFM4S_000837"
     CDS             complement(3632..4075)
                     /locus_tag="ACFM4S_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6050556.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAINAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(4082..4597)
                     /locus_tag="ACFM4S_000838"
     CDS             complement(4082..4597)
                     /locus_tag="ACFM4S_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6050557.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            4867..7497
                     /gene="leuS"
                     /locus_tag="ACFM4S_000839"
     CDS             4867..7497
                     /gene="leuS"
                     /locus_tag="ACFM4S_000839"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6050558.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQEFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            7727..8518
                     /locus_tag="ACFM4S_000840"
     CDS             7727..8518
                     /locus_tag="ACFM4S_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC6050559.1"
                     /translation="MNLFFDTKLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCSNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQIYEFENKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            8648..8779
                     /locus_tag="ACFM4S_000841"
     CDS             8648..8779
                     /locus_tag="ACFM4S_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HFC6050560.1"
                     /translation="MRDFDKLAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            8949..9764
                     /locus_tag="ACFM4S_000842"
     CDS             8949..9764
                     /locus_tag="ACFM4S_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFC6050561.1"
                     /translation="MLCLSAVISVLGACTVVADVYGQDSATMNAAAAKDYMKTVELNK
                     SAGNVDTTSRTARRVQAVFRRMLPYADAANNTGHRFDWKMTVFKNDELNAWAMPGGKM
                     AFYTGIVDKLKLTDDEIAAIMGHEMTHALHEHGKNKVGQQILTNTAAQIGTQIILDKK
                     PDTNPELVGLGMDILGTYGLTLPYSRSLEEEADEGGMMLMAQAGYHPAAAVRVWEKMN
                     QENDQNGFIYAITSTHPTNNARIENLKRLLPTVMPVYEQSVRNKGRVNKKRRR"
     gene            complement(10147..10458)
                     /locus_tag="ACFM4S_000843"
     CDS             complement(10147..10458)
                     /locus_tag="ACFM4S_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6050562.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(10455..10823)
                     /locus_tag="ACFM4S_000844"
     CDS             complement(10455..10823)
                     /locus_tag="ACFM4S_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6050563.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            complement(11633..11718)
                     /locus_tag="ACFM4S_000845"
     tRNA            complement(11633..11718)
                     /locus_tag="ACFM4S_000845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11682..11684),aa:Leu,seq:gag)
     gene            complement(11732..12082)
                     /gene="secG"
                     /locus_tag="ACFM4S_000846"
     CDS             complement(11732..12082)
                     /gene="secG"
                     /locus_tag="ACFM4S_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6050564.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(12089..12862)
                     /gene="tpiA"
                     /locus_tag="ACFM4S_000847"
     CDS             complement(12089..12862)
                     /gene="tpiA"
                     /locus_tag="ACFM4S_000847"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6050565.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            13046..13549
                     /locus_tag="ACFM4S_000848"
     CDS             13046..13549
                     /locus_tag="ACFM4S_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6050566.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADEADWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGETVVVGDVNA
                     YPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEYLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            13651..14307
                     /locus_tag="ACFM4S_000849"
     CDS             13651..14307
                     /locus_tag="ACFM4S_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6050567.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYAAALLAKLAGRV
                     VSDDIDAEQQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            14387..14710
                     /locus_tag="ACFM4S_000850"
     CDS             14387..14710
                     /locus_tag="ACFM4S_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6050568.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(14721..>14937)
                     /locus_tag="ACFM4S_000851"
     CDS             complement(14721..>14937)
                     /locus_tag="ACFM4S_000851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050569.1"
                     /translation="SLHIENAAQANFQFASLVLALSHGERGLGCRLPLRFRQILTSLI
                     PRFSFAEAALSDDLLEHHRYTHLRNAV"
BASE COUNT         3349 a         3743 c         4218 g         3627 t
ORIGIN      
        1 aatttaatcc actatacaaa ccgatttaaa ggagcgaaca tgttgatttt acaagccgaa
       61 cgcgacagcc tgctcaagcc gctgcaagcc gttaccggca tcgtcgaacg ccgccacacc
      121 ctgcccatcc tgtccaacgt cctgattgag ggaaaaggcg gtcagaccaa actcttggca
      181 accgatttgg aaatccaaat cgacactgcg ggtcccgagg gcggcgcgga cgacttccgc
      241 atcaccacca atgccaagaa atttcaggac attttgcgcg cgctgcctgc cggtgcgctg
      301 gtgtcgctgg attgggacga cagccgtctg acgctgaagg cgggcaaatc gcgttttgcc
      361 ctgcagaccc tgcctgccgc cgattttccg atgatgaatg tcggcgagga catcagcgcg
      421 actttctcgc tggagcagga gcgtttcaaa accatgctgt cgcaagtgca gtacagcatg
      481 gcggtgcagg acatccgcta ttatctcaac ggtctgctga tgcaggttga gggcagccag
      541 ttgcgccttg tggcgaccga cggacaccgc cttgcctatg cagcctgcgc gattgatgcg
      601 gatttgccgc gcgccgaagt gattttgccg cgcaaaacgg tgctggaact gttcaaactg
      661 ttgaacaacc ccgacgatcc gattcaaatc gagctgctgg acaagcaggt gcgtttccaa
      721 tgcaacggca cgaccatcgt cagcaaggtt atcgacggca aattccccga tttcaaccgc
      781 gtcatccctt tggacaacga caagattttc gttttgtccc gtgccgaact tttgggcgcg
      841 ctggaacgtg tgtccattct tgccaacgaa aaattccgtg gcgcgcgcct gttcctgcaa
      901 tccggcctgt tgagcgtcgt gtgcagcaac aacgagcagg aagaagcgcg cgaagaaatc
      961 gagattgcct atcagggcgg cgaactcgaa gtcggtttca atatcggcta tctgatggac
     1021 gtgttgcgca acatccattc cgacgatatg cagcttgcct tcggcgatgc caaccgctcg
     1081 acgctgttta ccgtgccgaa caatccgaat ttcaaatata ttgtgatgcc gatgcggatt
     1141 tgacggtttt ccggagcacg atgcccgtat tggagatatg ccccgaaccg tgcagacgga
     1201 ttcggggttt tgttcggctg tcggaaaggc aatgccgtct ggaatgcggc ggattggggt
     1261 tgggagcgta taggggaagt gcttgtgcgg gaatggcgaa ggtgtgtttt tgattccagt
     1321 ccgccctgtt tagattgctg taaaaccatg ccgtctgaaa cggaaaggtt tcagacggca
     1381 tggtttttga ttggggcggt gcgggtcagc ctccgagcag tgtccacaaa tcgttttgca
     1441 ggtcggcttc gctttcgccc tcggcaggcg cggcgcggat atagccgccg tcgggctgca
     1501 tcagccaggc gtgggtgttg tctgccaatg ccatttccaa accttcgcgg atcacgcgtt
     1561 ttttgagttc gggcgcggta atcggcgcgg cggtttcgat gcggcggaag aagttgcgcc
     1621 ccatccaatc cgcgctggaa ataaaggtat cgtccgcgcc gttgttgtgg aagcaataca
     1681 cgcgcgcgtg ttcgagctgc ctgccgacga tggagcggac gcggatgttt tcggacaagc
     1741 ctttcacgcc ggggcgcaga gtgcacatac cgcgcacaat caaatcgatt tgcacgcctg
     1801 ccgcgcttgc ccgatacagg gcttcgatga cggtcggttc gatgagcgaa ttcatcttgg
     1861 cagtaatccg tgccggcttg cctgctttgg cgtgttcggt ttcctgcctg atgcgtccga
     1921 taaccatttt gtgcagggta aacgggcttt gatagagctt gttcagacgg ccgggtttgc
     1981 ccaaacctgt gatttccata aacaatgtgt tcacatcggc ggtgatttgt tcgtcggcgg
     2041 taatgatgcc gaagtcggtg tagatgcgcg atgtgccttg gtggtagttg cccgtgccga
     2101 ggtgggcgta acgcttgagc acgccgtctt cgcggcggat gaccagtgcc atcttggcgt
     2161 ggattttgta gccgaacacg ccgtacacga cgtgcgcgcc cgcctcttcg agctgcttcg
     2221 cccagttgac gttgttggct tcgtcaaaac gcgccatcag ttcgacgacg acggttactt
     2281 gtttgccggc gagtgccgcc ttcatcaggg cgcggacgag ttcggagcgc gtgccggtgc
     2341 ggtaaatcgt cattttgacg gcaagcacgg cggggtcggc ggcggcttcg cgcatcattt
     2401 cgacaacggg gtcgaaggat tggtaggggt ggtgcagcag gatgggcgat tggcgcacca
     2461 aatcgaatat ggacgcggtt ttgcccaagg ctttcagcct gcccggcgtg tgcggcggga
     2521 atttcaaatc ggggcggttg accaaatcgg ggacggcgtt gaggcgcacg aggttcacgg
     2581 ggcctttgac ttggtaaagt tcgtcggcgg tcagcctgaa ttgcgacagc aggaagtcgc
     2641 ggatgcgggc gggacaggtg tcggcgactt cgaggcgcac gccgtcgccg tattcgcggt
     2701 cgtgcaactc gttttgaacg gcggcgcgga ggttttgcac gtcttcttca tcgacgttca
     2761 agtcgctgtc gcgcgtcagg cggaactggt ggcagccttt gacgttcatg cccgggaaga
     2821 gtttgccgac gtgggcgtgc aggatggagg agaggaagac gaagccgtgt ccgccgccac
     2881 acagttcgga cggcagggga acaacgcgcg gcaggatgcg cggtgcctgc acaatcgcca
     2941 tccccgaagg cctgccgaac gcgtccgtgc cgtcgagttc gacggcgaag ttgagcgatt
     3001 tgttcagcgg tcgcgggaag gggtgggacg ggtcgagtcc gatgggggtc aggatcggca
     3061 gcagttcgcg gtcgaaatag tcttcgatcc attttttctg cgcgcccgtc cagttgcggc
     3121 ggcggtaaaa atggatgctt tcccgcgcca actcgggctg aaggacgttg ttgaacaggt
     3181 cgtactggtg ccgtatcagg gagcgcgccg cttcggtaac gtcggcgatg gtttcagacg
     3241 gcattttgcc gttgtccagc ctgcgccggg ggtgcagctt gttttcacgc ttgagccacg
     3301 ccatgcggac ttcgaaaaac tcgtcaagat tggacgacac gatgcacagg aagcgcaggc
     3361 gttccaaaag ggggacgttt ttgtcttccg cctgtgccaa cacgcggcgg ttgaatgcca
     3421 gcaggcttag ttcgcggcag aggatgcggt tttgttcggg cataaggttc tcccaaagat
     3481 ggttgttgtt cggtcggagc gggcaggcaa tgccgtctga aggcttggcg gcatctgcgc
     3541 cggtttcaga cggcatgacg tggcggacag cctgtttcag gcatcggggc aatgtgaaaa
     3601 cagataccgg cgcgtgcggt atccgtctgt ttcaaatcac ttccaacaca aaataatgac
     3661 gcagtttttc gtacaccgca tcgctgacgt tgatgcagcc gttggtcata atcctgtcgg
     3721 acacggacgg ggaggcgatg cgttcgttcc gcctttccga cggtatctga ttccaaacgc
     3781 ggtgcagggc gaaaagaaaa tcaccctcct gcttgaaacc gatgacttcg ccgccgtatc
     3841 caggcttgtc ggtactgttc agcgtcagcc cgaacgtccc cttgggcgtg gcaacaccga
     3901 tgaggacggg gtggcactga cggtcgtcag cgaaacagag ttccgctttg gatgtatcga
     3961 cgatgacttt tttcttttgg atataggcat tgatcgcatc cggttgcccc tgtgcatata
     4021 cgggggcaac cattgacagg ccgcataata tccctaaaaa caggcggaac ggcatgtcgg
     4081 attattggcg gatacgtttc ggttttgccg gcacgatttc gcggatgatg acttgcggct
     4141 cggctttggg tgcgggcggc ggacaggcgg catctttcgg gaagacgggg ttccagtaga
     4201 agctgcgggc aaatttgtct ttatcgaaaa tcactttgta ttggcaggta gtaacatctt
     4261 ctacgccgga agtgttttca gggtcgatac ctacgcccgg ggtatggaag tggaacaggt
     4321 aatcccattc gcgcacgccg tacatacctt cgtcgtaatg cgggcggccc aggattttgt
     4381 agatgtcgtc tttggtcagg ccggggcgca tctgatccag ttcgtcataa gtcgggaatg
     4441 tgccgcgctt gttgtcgagc gttacggaat agggtttcgg gaaaaccgga ttgtcggtcg
     4501 tgccgtcggc tttgacgttg cttttggttg cgcaggcaga cagaacgccc gccgccaata
     4561 ctgccaagcc cggtttgacg atttgtttga ttttcatatg cagaatcctc ttttacccga
     4621 tgcccgtctg ccttttgttc ggaagagccg catcaggaga gttttaaaca tttgattttg
     4681 gtttgtaata ttagcataaa aaacgtgcgt atcaataaac gcggtgtatt tgtacggcat
     4741 acggaataat gcgcgtgcga atttacgcat cctgccggca atctgccgat tcgccgcttc
     4801 cggcaacctg ttataattga tgccgacttt atttttccaa cgtttttaag cgaaaacaga
     4861 atgactatgc aagaacaata tcaacccgcc gccatcgagc ctgcggcgca gaaaaaatgg
     4921 gacgacgccc gtattttcaa cgtctccgaa gacgcttcca aacccaaata ttattgcctc
     4981 tccatgttcc cctatcccag cggcaagctg catatggggc atgtgcgcaa ctacaccatc
     5041 ggcgatgtat tgagccgctt caaactttta aacggcttca acgtcatgca gcctatgggt
     5101 tgggacgcgt tcggcatgcc ggcggaaaac gcggcgatga aaaacaacgt cgcccctgcc
     5161 gcttggacgt acgataacat cgaatacatg aaaacccagc tcaaaagcct gggttttgcg
     5221 attgactggg cgcgcgaaac cgcaacctgc aaacccgaat actaccgctg ggaacaatgg
     5281 ctgtttacca agctgtttga aaaaggcatc gtctatcgca aaaacggcac ggtaaactgg
     5341 gatccggtcg accaaaccgt ccttgccaac gagcaagtca tcgacggacg cggctggcgt
     5401 tccggcgcat tgatcgaaaa acgcgaaatc ccgatgtatt acttcaaaat cacggattac
     5461 gccgaagagc tgctcaacga cttggacaag ctggaacact ggccggaaca agtcaaaacc
     5521 atgcagcgca actggatcgg taaatctcgc ggcatgaccg tgcgcttcgc cgtttcagac
     5581 gacagcaaac aaggtttgga aggcgattac gcgaaattcc tgcaagttta caccacccgc
     5641 cccgacacgc tgatgggcgc gacttatgtc gccgttgccg ccgagcatcc gctggcaacc
     5701 gccgcagccg ccgacaaacc tgaattgcaa gagtttatcg ccgaatgcaa agccggctcg
     5761 gtcgccgaag ccgatatggc gacgatggag aaaaaaggcg tgccgaccgg ccgctacgtc
     5821 gtcaacccgc tcaacggcga caagctggaa gtgtggattg ccaactatgt attgtggggc
     5881 tacggcgacg gcgcggtgat ggcggttccg gcgcacgacg aacgcgattt tgagttcgcc
     5941 accaaataca atctgccgaa aaaacaagtc attgccgtcg gcgacaacgc atttgacgaa
     6001 aaccaatggc aagaatggta cggcgacaaa gaaaacggcg tattggtcaa cagcggcgac
     6061 ttggacggct tggattttca gacggcattc gacgccgtcg ccgccaagct gcaaagccaa
     6121 ggcgcgggcg aaccgaaaac ccaataccgc ctgcgcgact ggggcatttc gcgccaacgc
     6181 tactggggct gcccgattcc catcgtccat tgcgaacaat gcggcgacgt acccgtccct
     6241 gccgaccaat tgccggtcgt tttgcctgaa aacgtcgtac ccgacggcat gggttcgccg
     6301 ctggcaaaaa tgcccgagtt ttacgaaact gcctgcccat gctgcggcgg cgcggcgaaa
     6361 cgcgaaaccg acaccatgga caccttcatg gaatcgagct ggtatttctt ccgctacatg
     6421 tcgccaaaat tttcagacgg catggtcgat cctgccgccg cgaaatactg gggcgcggtc
     6481 gaccaataca tcggcggcat cgaacacgcg attttgcacc tcttgtacgc gcgtttcttc
     6541 accaaactga tgcgcgacga aggtctagtc aatgttgacg aaccgtttga acgcctgctg
     6601 acgcaaggca tggtcgtttg cgaaacctac taccgcgaaa acgacaaagg cggcaaagat
     6661 tggatcaacc ccgccgatgt cgagctgacg ttcgacgaca aaggccgccc catttccgcc
     6721 gtcctcaaag ccgacgggct gcccgtcgtc atcagcggca cggaaaaaat gtccaagtcc
     6781 aaaaacaacg gcgtcgatcc gcaagaactg attaacgcct acggcgcgga caccgcccgc
     6841 ctgtttatga tgttcgccgc accgcccgaa cagtccctcg aatggagcga cagcggcgtc
     6901 gaaggcgcac accgcttcct gcgccgcctg tggcgtaccg tttacgaata cttgaagcaa
     6961 ggcgaagcgg tcaaagcgtt cgcaggcagc caagacggtt tgtctaaaga actcaaagac
     7021 ctgcgccaca aactgcacgc gaccactgcc aaagtcagcg acgactacgg ccgccgccag
     7081 cagttcaaca ccgccatcgc cgccgtgatg gaattgctca accaatacga caaaaccgac
     7141 accggcggcg aacaaggacg cgccgtcgcc caagaagtat tggaaaccgc cgtacgcctg
     7201 ttgtggccca tcgtgccgca catctgcgaa accctgtgga gcgaattgaa cggcgcgaaa
     7261 ctgtgggaag caggctggcc gacagtcgac gaagccgcct tggtcaaatc cgaaatcgaa
     7321 gtgatggttc aagtcaacgg caaactgcgc ggcaaaatca ccgtcgccgc cgacgcctcc
     7381 aaagccgacc tcgaagccgc cgcccttgcc actgaaggcg cggtgaaatt catggaaggc
     7441 aagcccgcga agaaaatcat cgtcgtaccg ggcagactgg tgaacatcgt cgtctaaacc
     7501 gctcttaagg tttagccaca cggataaagg ccgtctgaaa cttggaaaca gggtttcaga
     7561 cggccttttt aaggcagatt gggttgtctg cattacagac ctcaaatata gtggattaac
     7621 tttaaaccgg tacgacgttg ccccgccttg ccctgctgtc tgcggcttcg tcgccttgtc
     7681 ctgatttttg ttaatccact aaaactgaaa tctcaaaatc caaaacatga atttattttt
     7741 cgataccaaa ttgggaaagc aacaaaataa agcaacccat aaaatccgtg taatgagtga
     7801 ggcttggctg gaaaaaaacg gctactgccc ctgttgcgga agcaagccga tgcagagatt
     7861 tgccaataac aaacctgttg cagacctctt ttgctcaaat tgccacgagc aatatgaatt
     7921 aaagagtaaa aatcaaaaaa ccataggtaa cagtgtgcct gacggtgcat atcgcactat
     7981 gttggagcgc atccggtcag ataccaaccc caactttttc tttcttgcat ataaaaaagc
     8041 ggattactct atacggcaat tggtgcttgt acccaaacat ttcatcacgc cggacatgat
     8101 tattcccaga aataaaggca ttaaaaaccg gccgcaccac attatgtgtt ccatcaatct
     8161 cgcccctttg cccgaaagcg gcaaaatatt cttaatagac gattcccgca ttatcgaacc
     8221 cgaaaccgtt ctgaaaaaat ggcaatccaa tctgttttta cgcaaccaaa atgcggagcg
     8281 caaaggctgg cttttggctg ttatgaaatg tatcgaccaa cttcccgaag aattcacatt
     8341 gtcgcaaata tatgaatttg aaaacaaact atccatccaa tttccccaaa acaaccatat
     8401 cagagacaaa atccgccaac agttgcaaat tttgcgcgac caaaatacga tcgaattcat
     8461 tggtcgcgga ctttacaaaa aaatcgacaa attgcccccg acttccaagg cgttttaatt
     8521 tcaaatcatg atactgaatt cacaagggat atttattctt ctgttattct gaaaacagtt
     8581 gaagtacagc acaatattgc cgcaaaaggc agcagccgta agaaggtcgg ggaattattg
     8641 gcaatttatg cgtgatttcg acaaattggc tgaagaagct gccgaacacg ccgctgaagg
     8701 cgcggtgaaa ttcatagacg gcaagcctgc caagaaaatc atcgccgtac cgggcagact
     8761 ggcgaacatc gtggtgtaaa tctttttaaa aacatcaaac aatgttaaga tgccgtccga
     8821 aatccgtttt agattcagac ggcatttttt aaatttaatt ttttaagggg taagcctaat
     8881 atgaaatttc cttccttcct tccttccttc cttccttcct tccttccttc cttccttcct
     8941 ttaaacggat actctgcctg tcggcagtaa tctcggtatt gggggcttgt acggtcgttg
     9001 ctgatgttta cggtcaggat tccgccacaa tgaacgctgc ggctgccaaa gattatatga
     9061 aaacggttga gttgaacaag tctgccggca atgtcgatac cacatccaga acagcccgca
     9121 gggtgcaggc agtatttcga cgtatgctgc cttatgccga tgcggcaaat aataccggcc
     9181 ataggtttga ctggaaaatg acggttttca aaaacgatga gctgaacgcg tgggcgatgc
     9241 ccggtggaaa aatggcgttt tatacgggga tagtcgacaa actcaagctg accgatgacg
     9301 aaattgccgc cattatgggg catgaaatga cgcacgccct gcatgaacac ggtaaaaata
     9361 aggtcgggca gcaaatcttg accaatacgg cggcgcagat aggcacgcag attatattag
     9421 acaaaaaacc ggatactaat ccggaattgg tcggattggg tatggatatt ttggggacgt
     9481 acggtcttac cttgccttat agccgcagct tggaagaaga agccgatgag gggggaatga
     9541 tgttgatggc gcaggcaggc tatcatccgg cggccgctgt cagggtttgg gaaaaaatga
     9601 atcaggaaaa cgaccaaaac ggctttattt atgctattac ctctactcat ccgacaaaca
     9661 atgcccgtat agaaaatcta aaacggttgt tgccgaccgt tatgccggtt tatgagcaaa
     9721 gtgtcagaaa taaggggcgc gttaataaaa aacgtcggcg ttaagggaag gggatcgaga
     9781 atttgagcca ttgtttcaaa atgccgtctg aaatcgtttg agattcagac tgcatttaaa
     9841 cagttctgtg ccccacccgt tttgccttca agcccgcgcc gcttaaatcc aatccccccc
     9901 tgcaacgggc gcaaaaaatc cccgtacctc gcggtcggtg atgtttcggg tttcgcgccc
     9961 gtctgcgccg tgccttgctg tcgggctatg tcgtctgcac cgccgccgtt tcggcttggt
    10021 ttgatgcggt cgggcattta tgcggctacg ccctgatatt cagttcggcg gtcttgcctg
    10081 tcttggcagg catattcagc agcgcgtcta ttgtgaactt gctgattttg ccgtttgcca
    10141 agtcggctac gcaatggctt cgatgccctt gtttttgacg atggtcagca gcttcaacgc
    10201 cgcgccgctc ggctttttaa cgccccgctc ccaagccgaa acgtgatttt ttcccacgtt
    10261 gagatagatg gcgaaagcgg cttgcgatag tgcctccttc tccctgattg ccttgatgtc
    10321 cccgccgctc aacggtttga tttcggtcag gcaggacttg tcaaagccgc gcatagtctt
    10381 tttgtcgatt gcgccgatgt tgtaagcccc ttccatcatt tcatgaatgg cggctaatgc
    10441 ctcgcttttg tatttcatga ttctatctcc gtgataatgc cgtcttcttt caaagccgcc
    10501 agctctgccc gcgtgtattt cagataatat gcggcggctt ttcggtaaac gtccagttct
    10561 ttatccgaaa tgttttccct gtcgttcttg gcaaaggcgt aaacgaaaaa tgccctatct
    10621 gcctgtttga acaatatcag gctgcgatag ccgccgcttc tgccttgtcc ttgccttgct
    10681 atgcgctgct taatcacacc gccgcctaaa tctgcgtcta tcagcccgtt atccgcccgc
    10741 tctaccgctt ccagcagctc ggaatcgttg attttgtgct tcttggcaaa tttcactatc
    10801 cattggtttt tgaatatccg cattgtgtag ccgttgatta gtgatacttg gaattatacc
    10861 agagacaggc aaggcggcaa gtcgcctttt tataccctgc aatttctccg tttatgccgc
    10921 ctgaaacacc ggcggctaac ctgattattt tttaatcaat cgcttttaat ggctcaaatt
    10981 gcgtttttag cgcgtttcgc tgtcttccta tgccagtata taaccaagcg gtttaaccgc
    11041 cattcctgct caaaataggc gcggttccgt gcgttttggc ttttcattcc gctacgctcc
    11101 cactgccaag acggtgaaaa accggaaaac cccaaaagtt gttaccgatt cgttaccatt
    11161 tgttaccgca tttgttacca attttttatc tttttcttat tttatagtgg attaaattta
    11221 aaccggtacg gcaaggcgag gcaacgccgt accggtttaa atttaatcca ctatagtata
    11281 ggttttttcc aaaaacccaa aaaaaaccgc tctgcatctt ggacggtttc cccctcattc
    11341 cctacggtgc gtcagtattc cccgcaagct atttcccgct tggcattcct gatttggtgg
    11401 tcgttcagct ttatttttgc ggtatttttt cgaggtattt ttcagatacc gcaaaagata
    11461 ccgtaaattt taggttactt caattaggct gaattaaacg gaattataca atcaggaaga
    11521 agacaatagg atagcaacca gtctgaaagc cttgtattga ttgggattgg ttgacggcat
    11581 tagacaggat taaacgaaaa aaacgcttag aatttctaag cgttttgtgt gttggtgccg
    11641 acagcgagat ttgaactcgc acagcctacg gccactaccc cctcaagata gcgtgtctac
    11701 caatttcacc atgtcggcat ttgaaaaact gttatttctg ctgctgagga acaggggcag
    11761 aaggttcggt attgcttacg ggtttgggtg cttgctgagt ttgttgtacg ttgctgaagt
    11821 ccaaaccgtg ttttgttgtg tgggtgtgaa tatacaccat agccatgcag gttgcaaaga
    11881 aaaatgttgc tgcaacggcg gtcgagcggc tgaggaagtt agcgttgccg gcagagccga
    11941 atacgccttg cgcgctgccg cttcccgatc cgaaagtcgc gccggcatcc gcgcctttgc
    12001 cgtgttggag caatactaac acgatgacgg ccaaagcgga aattatatta acaatccaaa
    12061 ttagggtttt gaaggcttcc atatttttct acgcattttg tgcggcactg atgatggcgg
    12121 taaaggagtc gtacgacaat gacgcgccgc cgacgagtgc gccgtccaca taaggtactg
    12181 cgaagatgtc ggccgcgttg tccgctttca cacttccgcc gtaaaggaca cggattttaa
    12241 catcgcttcc gcacaaagac aagatttctt tgtagatgaa tgcgtgcata tcggcaatct
    12301 gttcgacggt ggcgactttg ccggtgccga ttgcccagac gggttcgtag gcgacggcga
    12361 tgtttttggt gtccagtcct tggaggatgg aaagctgatg ggcgatgact tcgtgttctt
    12421 tgccggcttc gcgctcttcg aggctttcgc cgacgcacaa taacgggatg agtccgacgt
    12481 tgaggacgtt ttccattttg cggcgttgga tttcgttttt ttcgccgaaa taaaggctgc
    12541 gttcggagtg tccgatgagg acgatgtctg tgccggtgtc ggcgagcatt tcggcggaca
    12601 cttcgccggt gtacgcgccg ttgtcgggga agcggctcac gtcttgggcg caggtgagga
    12661 tgcggttgtt taagacgatt tgcatggcgt tgtgcagttg cagcaggtaa acggtcgggg
    12721 cggcgagtcc gatgaggacg cgttcggcgg tggggaggat gcggaagcgg tgcatcagtg
    12781 cgttgttgtt ttggagccgg ccgttcattt tccagttgcc gatgacccat ttttgatccc
    12841 acattccgat ttggcgatac atcttttttg ctccgtgtcg tgttttttct gtctgccgcg
    12901 tgtggcgcgt tgcaatgtga agtttagtgg atatgcggcg ggttcgcaac ttggggcggg
    12961 cggctgcggg ggcggttcgg aatgttgttt cgggcaggtt gttttataat ggccgcctga
    13021 tatgtatgca actataggag atgtgatgca cgcgcttcat ttttcggctt cggacaaggc
    13081 cgcgctttat cgggaggtgt tgccgcagat tgagtctgtg gtggctgacg aggcggattg
    13141 ggtggcgaat ttggcgaaca cggcggcggt tttgaaggag gcgttcggtt ggttttgggt
    13201 gggtttttat ttggtcgata cgcgttcgga cgaattggtt ttggcaccgt ttcaggggcc
    13261 tttggcgtgt acgcggattc cgttcggtcg cggggtgtgc ggtcaggctt gggcgaaggg
    13321 agagacggtg gtcgtcgggg atgtcaacgc gtatcccgac catattgcct gttcgtcttt
    13381 gtcgcgttcg gagattgtcg tgccgctgtt ttcagacggc cgctgtatcg gcgtgttgga
    13441 cgcggacagc gaatatttgg cgcagtttga tgagacagat gctttgtatt tgggcgagct
    13501 ggcgaagatt ttggagaagc ggtttgaggc ttcgcgtcag gcggtttgag tgagggaaag
    13561 gggcgggcgg cgcgtgccga agttggcgcg gcgggagtgt ggttttataa tgcctgctat
    13621 tgttggaaca attatttgac ggagcactaa atggattttg aaaaagcgcg gttcaatatg
    13681 gtcgaacagc aaatccgtcc ttgggatgta ttggattttg acgttttgga cgctttggcg
    13741 gagattccgc gcgagctttt tgtcgatgag gacttgcagg gtttggcgta tgcggatatg
    13801 gcgctgccgc ttgccaacgg gcataagatg ctcgagccga aagttgtggc gcggctggcg
    13861 cagggcttga agctgacgaa aaacgatacg gttttggaaa tcggcacggg atcgggctat
    13921 gcggccgcgc tgttggcgaa actggctggt cgtgtggttt cggacgacat cgatgccgaa
    13981 cagcaaaagc gcgccaaagc ggttttggac ggcttgtctt tggaaaacat tgattatgtg
    14041 caaaacaacg ggctgaccga actttccgca ggcgcgccgt ttgatgcggt ttatgtcggc
    14101 ggcgcggtaa accttgtgcc tgaagtgttg aaagaacagt tgaaagatgg cggacgcatg
    14161 gcggttatcg taggccgcaa gcctgttcag cgcgcgcttt tgattacgcg caagggcgat
    14221 gtgtttgaag agaaggtgct gttcgatact ttggtggcgc atttggatga taaggatgcc
    14281 gatccgtttg gcggtttcga tttttaagat gttgacaggt catgccgtct gaaagcgggt
    14341 ttggggcttc agacggcatt ttgcttggtt tttttggggg ggtgcgatgg atattgtgca
    14401 acttgggccc gcggaattga aagcgtggat ggacgaaggg cggatgtttt gtttgttgga
    14461 cgtgcgtacg gatgaagaaa cggcggtttg ttcgctgcca aatgcgctgc atatcccgat
    14521 gaatctgatt ccgctgcggc agaacgagct gccggatgat gtgccgcttg tggtgtattg
    14581 ccatcacggt atccgcagtc tgcatacggc gatgtatttg gcggacgcgg gttttgaaaa
    14641 cctgtacaac cttcagggcg gcatcgatgc atgggcggcc gagattgacg gcaacatgat
    14701 gcgttattga ggcaggcatt tcagacggca ttccttaaat gcgtgtacct gtggtgttcc
    14761 aaaaggtcgt ctgaaagtgc agcttctgcg aagctaaagc gcggtatcaa cgaagttaaa
    14821 atttgcctga accttaaagg cagcctgcac cccaatcccc tctccccgtg ggagagggct
    14881 aggacgaggc tggcaaactg aaagtttgct tgggcggcat tttcaatgtg caggctg
//
LOCUS       DAXHMX010000038        14925 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHMX010000038 DAXHMX010000000
VERSION     DAXHMX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.038"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(97..3582)
                     /gene="smc"
                     /locus_tag="ACFM4S_000852"
     CDS             complement(97..3582)
                     /gene="smc"
                     /locus_tag="ACFM4S_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6050570.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATEQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATGHAAAPQLWQHITAPAEWQHALSVILAERLHARDVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLSPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHASDGLSDDIATLQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIARLEQQTLDWQERQQELVLAYETEFQNDEQHIKLDELT
                     ESVHTLDEEYIAVQEKLTQIQEQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQTAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAESV"
     gene            3831..4553
                     /gene="lpxH"
                     /locus_tag="ACFM4S_000853"
     CDS             3831..4553
                     /gene="lpxH"
                     /locus_tag="ACFM4S_000853"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6050571.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4822..6408
                     /locus_tag="ACFM4S_000854"
     CDS             4822..6408
                     /locus_tag="ACFM4S_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6050572.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6576..6779)
                     /locus_tag="ACFM4S_000855"
     CDS             complement(6576..6779)
                     /locus_tag="ACFM4S_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6050573.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6895..7392
                     /locus_tag="ACFM4S_000856"
     CDS             6895..7392
                     /locus_tag="ACFM4S_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6050574.1"
                     /translation="MTALTLPEDILQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADEGRDIPSLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIRSNWETFA
                     HIGQA"
     gene            7474..8667
                     /locus_tag="ACFM4S_000857"
     CDS             7474..8667
                     /locus_tag="ACFM4S_000857"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6050575.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIATEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9070..10005)
                     /gene="hemC"
                     /locus_tag="ACFM4S_000858"
     CDS             complement(9070..10005)
                     /gene="hemC"
                     /locus_tag="ACFM4S_000858"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6050576.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVILQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10124..10639
                     /gene="ybeY"
                     /locus_tag="ACFM4S_000859"
     CDS             10124..10639
                     /gene="ybeY"
                     /locus_tag="ACFM4S_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6050577.1"
                     /translation="MKRAKQYPFLSLQRQRFHLNFENASSTANLPSERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10641..11465
                     /locus_tag="ACFM4S_000860"
     CDS             10641..11465
                     /locus_tag="ACFM4S_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6050578.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11608..12987)
                     /gene="nhaC"
                     /locus_tag="ACFM4S_000861"
     CDS             complement(11608..12987)
                     /gene="nhaC"
                     /locus_tag="ACFM4S_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6050579.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNGVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13279..14502)
                     /locus_tag="ACFM4S_000862"
     CDS             complement(13279..14502)
                     /locus_tag="ACFM4S_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6050580.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3524 a         4190 c         3844 g         3367 t
ORIGIN      
        1 gctgtaccga tttaaattta attcactata ccgtctgaca acccgtatcg caacacttca
       61 ttaaaaacgg caagattcag ccgttctgca acccgttcaa acggattccg ccatttccaa
      121 cgcctgtttg atgtccacgg cgacgacgcg cgatacgcct ttttcctgca tggttacacc
      181 gaccagctgt tccgccattt ccatggtcag gcggttgtgg gagatgtaga gaaactgggt
      241 ttgcgccgac atttctttga ccaggttgca gaaacgcgaa gtgttggcat cgtctagcgg
      301 agcgtcgact tcgtccaaaa gacagaacgg agcggggttg aggctgaaca gggcgaacac
      361 gaggctcatc gcggtaaggg ctttttcgcc gccggaaagg aggtggatgg tgctgttttt
      421 cttgccgggc gggcgcgcca tgatagacac gccggcggtc aaaaggtcgt cgcctatcat
      481 tttgagggtg gcttcgccgc cgccgaacag ggtcgggaag aaggtttgga ctttgccgtt
      541 gacggcgtcg aaggtttctt tgaaacgcgc tttggttttg tcgtcgattt gggcgatggc
      601 ttcttccaaa agggtgatgg cggtctgcac gtcttcgctt tggctgcggt agtagccgtc
      661 gcgttcgcgc gcttcttcga gttcttgcag ggcggcgagg ttgaccgcgc cgagggcttc
      721 gatttgttgc gagaggctgc cgatgctgct gttcaatact ttcggcgatt ctttcgccaa
      781 cgcttcgagc gcatccaaat cggcggcgcg ttcggtcagg ttttgatggt agcgtttggc
      841 gttgatcagg gcttcctgtt gttgcaacag ggcggtttgg gtggcggcct gaagctgtgg
      901 caactgggtt tgcagggttt gtacgcgggc gtattgttcc ctaccctgct cctgaatctg
      961 cgtgagtttt tcctgcacgg cgatgtattc ttcgtccagc gtgtgtacgg attcggtcag
     1021 ctcgtcgagc ttgatgtgct gctcgtcgtt ttggaactcg gtttcatagg cgaggacaag
     1081 ctcttgctgg cgttcctgcc agtctagggt ttgctgttcg agtcgggcga tttgctgctg
     1141 gtagttttgt ttttgctggt tgagtttgtg gacggcgact tcggcaagcc catattggcg
     1201 gttggcttcc agcagggcaa gctgcgcctg tttcagacgg ccttgctgct cttggcggct
     1261 gtgtgcggcg gtttgctgct ggtgttcgag ttcggcggcg gcttcctgca aagtagcaat
     1321 gtcgtctgaa agcccgtcgg acgcgtgttg caacacggtc tgctcttccg ccaactgcgc
     1381 cagttcgcgc tcgatgtgtt cgcggcggat ttgcccttgg ttggtacgcg ccagaagttc
     1441 ggcggcgcgt tgctgcgctt ggctgtattg gcgcgtgtgc tgctgttgct gctgcatcag
     1501 gtttttatgc tgcacttcgg acgagcgcac ggcggcttcc gcctgtttga acgcggcttc
     1561 ggcggcagaa agttcggggg agaggttttc cagttcggac gcgatgccgt cgaggcgcgc
     1621 tttttgggca atcaggcttt cctgcgcggg tttggcatag agcaggacgc tgaccttatc
     1681 gacctgatgg ccttcgggcg tgagccagat ttgatgtgcg cccaaatcgt tttgatgcgc
     1741 gagggcgtaa ctcaaatcag gcgcacacaa tacgccgtcg agccagtggt gcaatgccgt
     1801 ctgaaacggc ggctgcgctt ggatttggtt cagcaatgcc tgtacgggca gggatttttt
     1861 gatgccgccg gagagaccgt ctgaaagcca cgccgcctgc ccctgcggca aaggttcggg
     1921 cggtacgaaa ccttgcggca cgtcgcgggc gtgcaggcgt tcggcaagga tgacggacag
     1981 ggcgtgctgc cactcagcag gcgcggtaat gtgttgccac agttgcggcg cggcggcgtg
     2041 gccggttgcc tgccagaaat cggctgcttc ctgctgttgc gacaggattt gcgacaacgc
     2101 ctgctgctgc gcctgcaagg tgatgtgttg ctgcttcagg ctttggaagc ggtttgaggc
     2161 cgtctgaaac gcctcgcggg cggcgtgtaa ggcttcttcg gcggcgatga tttgttcttc
     2221 gtaatgctct tgccggcttt gcaacaaggc agcggcctcc tgcgcggcgg cggtttccgc
     2281 ttcgtcgggc aggttcaggg cttggttctc ctgtttcaga cggcctttgc gctcttcgtg
     2341 cttggcaacg gtttgttcgg catgggcaag ctgctgctgc ttcaacgcca gttcgcggcg
     2401 gatgcggttt gcctcgtcct gctgggtttg gaaggcggca ttgagcgcgg tttgggtttc
     2461 ttccaattcg ggcagacgct cttcgtgttc ggcaacctgc atcgcccact ccgccagctc
     2521 ggtttgcttt tcttcgacgt ataactcgtt ttcttcaagc tgcacgcgga tttgctgctg
     2581 ctcttgatga atacgctgca actgcgcctg cgccgcctgc ttgtcgcgtt cgatgcgttg
     2641 gtgcaggttt tgctgatggc ggatttgttc ttccaaacgg gcaatctgct cgcgcaacac
     2701 gccgcgtttg ttgctcaatt catgcaccgc ctgttgctgc gactgttcgg cggtctgcaa
     2761 ggcgtgtact tcgtcgttta acgcctgaac ctgcgcggcg gtttcgtcct gctgcgcctg
     2821 caaagactga tgctgctcgg tcgccttgtc ggcggcggca agcgattgct gccattgggc
     2881 gtaatcgagc aaatcctgtt gccggttcaa ctgcgcggtc agggatttgt agcgttcggc
     2941 ggtttccgcc tgtttctcca gcttttccac ctgacgcgcc aattcgttct gcaaatcgcc
     3001 caaacgctgc aaatgctcgc gcgtgtcttt cagacggcct tccgtctcct tgcggcgttc
     3061 cttatatttg gacacacctg ccgcctcctc gatataggcg cgcaattcct ccggccgcgc
     3121 ttcgatgatg cgcgaaatca tcccctgctc gataacggca taaccgcgcg cgcccacgcc
     3181 cgtacccaaa aataaatcgg taatgtcgcg gcggcgcacg gtctgattat tgatgaaata
     3241 agtcgattcg ccctgacgcg tcagctgccg cttgatactc acctcggcat actgccccca
     3301 cgccccctgc aaactgtggt cgctgttgtc aaacaccagc tccaccgaag ccctcggcgc
     3361 aggacggcgc gtcgccgcac cgttaaaaat cacgtcctgc atactctcgc cacgaagctg
     3421 cttcgccgaa gcctcgccca acacccagcg caccgcgtca atcacattcg acttgccgca
     3481 gccgttcggc ccgataaccg caacaagctg ccccggaaca tgaatcgtgg tcgggtcggt
     3541 aaaagatttg aagccggaga gtttgatgtg ggtcaggcgc ataatggtcg gaaaaaataa
     3601 aaaagaagcg tattttaacg gaaatcccgc cgcaccaccc catatcttgc cggcaaaacc
     3661 ttaccgcatc ccgcacccgc gatgccgtct gaagccttca gacggcattt ttaacgcgcc
     3721 cgaaccccgg ttccgccaat gcccgatgct tggcgcggca tttgccgtat tgccccgccg
     3781 ccgggcgcgt tataatccgc cccgacatcc gcccgattgg aaacctgcct atgaaacccg
     3841 cctatttcat ctccgacctg catttgagcg aaaagcaccc cgaactgacc gcgctgctgc
     3901 tgcgtttttt acgttcttcc gccgccgggc aggcgcgggc gatttatatt ttgggcgatt
     3961 tgtttgattt ttgggtgggc gatgacgaag tttccgagtt gaatacttcg gttgcgcggg
     4021 aaatcaggaa attgtccgac aaaggcgttg ccgtgttctt cgtcaggggc aaccgcgact
     4081 tcctgatcgg tcaggatttt tgccggcagg cgggtatgac gctgctgccg gattactcgg
     4141 ttttggactt gttcggctgc aaaaccctca tctgccacgg cgacactctg tgtacggatg
     4201 acagggcata ccaacgtttc cgcaaaatcg tgcatcggaa gcggctgcaa aaactgttcc
     4261 tgatgctgcc cctgaagtgg cgcacgcgcc ttgccgccaa aatcaggcgc gtcagcaaaa
     4321 tggaaaaaca ggtcaagccc gccgatatta tggatgtcaa tgccgccttt accgcgcggc
     4381 aggttcgcgc ctttaacgcg gaaaggctga tacacggaca cacccaccgc gagcatatcc
     4441 atcacgaaaa cggctttacc cgcatcgttt tgggcgactg gcataacgac tatgcttcaa
     4501 tcctccgcgt ggacggggac ggcgcggtat tcgtgccgct ggaaaaatgc tgaaaatgcc
     4561 gtctgaagcc tttcgggcgg ctttttttac acgccccttt cccacactcc ctccgccgca
     4621 tcatcaccat ccttaacccg cattaatggg cgttatcgat aaaatgcttg accgattaca
     4681 taagattacg taaagtgtgc aaacgcataa caattggtct taccaattta tcttttaacc
     4741 aaaatatcgg gtgtacggga tttgaacgtc tttccaaacc tcgacgcgcc ttatgatgca
     4801 aatgcctaaa aaggattcgt tatggcactt ttcctcagca tattccccat cgtcctgctg
     4861 atttggctga tggtgaaaaa aaacagtatg ccctcctacg tcgcgctgcc gattaccgca
     4921 gtgctgattt acgccatcaa acttttctac ttcggcgatg cgggcatgct gctcaacgcc
     4981 accgccgctt ccggcctcgt caaaacgctc acgccgatta ccgtgatttt cggcgcgatt
     5041 atgttcaacc gtatgatgga aaccacgggc tgcattgatg tcatccgcaa atggctggcg
     5101 accatcagcc ccaaccccgt agcgcaactg atgattatcg gctgggcttt tgcctttatg
     5161 attgaaggcg catccggctt cggcacgcct gccgcgattg ccgcgccgat tctgatgagc
     5221 ttgggcttca acccgttgaa agtggcgatt ttcactttgg tgatgaactc cgtccccgtc
     5281 tctttcggcg cggtcggtac gccgacctgg ttcggtttcg caccgctgaa cctgagtgcc
     5341 gaagacatcc tcgccatcgg caggcagacc ggcgtaatgc acttcttcgc aggtttcgtc
     5401 atccccgtca tcggcttggg cttcatcgta ccttggtctg aaatccgcaa aaacttgggc
     5461 ttcgtcgcca ttgccgtctt ctcctgcacc attccttatg tcgcattggc gatggtcaac
     5521 gaagaattcc cgtcgctcgt cgccggcgcg atcggcctga tggtgtccgt attcgccgcc
     5581 aaccaaggct ggggcttgag caaggaccac gccaaagacc cgaatgccga aaaagtgccg
     5641 ttcgcccaag tcgccaaagc actcgcccct ttgggtatgc tgatcggcat gctggtggtt
     5701 acgcgcatca aacagctcgg tatcaaaggc attttgacca gcaaagaaga atggttcagc
     5761 ttccaactgc cgtttgattt gtccaaaatc accgtcagcg actccctgac gattaccttc
     5821 ggcaatattt tcggacaaga tgtcagcgcg tcttaccaaa cgctgtacgt cccggcttgg
     5881 attccgtttg tgctgaccgt ttggatttgc atcctgctgt ataaaaccaa attcaaagat
     5941 gcctggacga tttatgccgt aaccttcaat caaaccaaaa aaccgctgct tgccctgatg
     6001 ggcgcgctga ttatggttca gctgatgctg gtcggcggcg acaattcgat ggtgaaaatc
     6061 atcggtaagg aatttgccgc aatggcgggc gaacactggg tttatttctc gccgtatctg
     6121 ggcgcgatcg gtgcgttctt ctccggctcc aacaccgtgt ccaacctgac tttcggcccg
     6181 attcagcagc aaatcgcctt ggataccggc ctgtccgtta ccctgattct ggcgttgcag
     6241 tccgtcggcg gcgcgatggg caatatggtg tgcctcaaca acatcatcgc cgtatgtacc
     6301 gtattggatg tgaaaaattc cgaaggtgcg attatcaaga aaaccgttat cccgatggcg
     6361 atttacggcg tgattgccgt cgtcgcggca atgattttct tcctctaggc gaaacgccac
     6421 catccatagc ggcagcacgc ggggaacgga aaccgccgtt ccccgtctgg gcggcgcgtc
     6481 gggcagaatc ccgctgccgg aacagcccgc cccgccaaac aaatgccgtc tgaaaccgga
     6541 aaaggcttca gacggcattt ttccgccgtt tgcattcagg cgtaatgatg atgcggcatc
     6601 ttgccgtcaa ggcggtagcg cgtgccgcaa tacgggcagg caacgctgcc cgattctcct
     6661 tcgcacaaag gcaaaaacac tctcggatgc ccgttccact gctcgttgtc gggtcccgag
     6721 caatacagcg gcagattttc cggcaacacg gaaatttcct gcagattcag attgtccatt
     6781 tgattttcct ttgcgtggtt ggcgtgcgcc cattttacgc catcggcagg ctaaaggata
     6841 ttttcggcgc aaagccgcaa tccgctataa tccccacttt tcagacggca taccatgact
     6901 gcgcttaccc ttccggaaga catcctccaa caagaaccat ccgttctgct ctataccctc
     6961 gtttccgcct acctcgaaca caccgcccaa accggcgacg aatccctctc ctgcctgtcc
     7021 gacgaccagc acacgctgac cgcattctgc tacctcgaca gccaagtcga agaaggcggc
     7081 ttcgtgcaac tcatcgcctc cggttacggc gaatatattt tccgcaaccc gcttgccgac
     7141 agcctgcgcc gctggaaaat caaagccgtg ccgaaagtct tggacaaagc caaagccctc
     7201 tacgaacaac acggcgaaac catcgaaacg cttgccgacg aagggcggga catcccttcc
     7261 ctgcgcaaac agttccccga ctttgaagaa tgggacggcg catactacga agccgccgaa
     7321 caagacctgc ccctgcttgc agaacacata cggtcaaact gggaaacctt cgcccatatc
     7381 gggcaggcgt gattgcgtct gtttccagcc cgtgtaaaac agcgtaaaat cggcaaaccc
     7441 gaatgacctt ccgtccccat caaggagcaa gctatgttct tcaagcacat cgaagccgcc
     7501 cccgccgatc cgattctcgg tttgggcgaa gcattcaaag ccgaaacccg ccccgaaaaa
     7561 gtcaacctcg gcatcggcgt ttataaagac gcatccggcg cgacacccct cgtcaaagcc
     7621 gtcaaagaag ccgaaaaacg cctgttggaa agcgaaacca ccaaaaacta cctcaccatc
     7681 gacggtgttg ccgactacaa cgagcaaacc caaatcctgc tgtttggcaa agaccacgaa
     7741 atcatcgcca gccgccgcgc caaaacagcg caaagccttg gcggtacagg cgcattgcgt
     7801 attgcgaccg agtttgccaa acgtcagttg aacgcgcaaa ccatctggat ttccaatccg
     7861 acttggccaa accacaacgc catcgccaaa gcggtcggta tccaagacaa accttatcgt
     7921 tactatgatg ccgccaaaca cggtttggat tgggacggta tgattgaaga cttgagccaa
     7981 gcgcaaaaag gcgacatcgt cctgctgcac ggctgctgcc acaaccctac cggcatcgac
     8041 cctacgcccg aacaatggga aactctggca aaactttctg ccgaaaaagg ctggctgccg
     8101 ctgtttgact ttgcctacca aggcttcggc aacggtttgg aagaagatgc ctacggcttg
     8161 cgcgtgttct tgaaacacaa tacagaattg ctgattgcca gctcttattc caaaaacttc
     8221 ggtatgtaca acgagcgcgt cggcgcgttc actttggtgg ccgaagatga agaaacagca
     8281 gcccgcgccc acagccaagt caaaaccatc atccgtacct tgtattccaa cccggcttca
     8341 cacggtgcga acaccattgc gctggtgttg aaaaatgatg atttgaaagc acaatggatt
     8401 gccgaactcg atgaaatgcg cggccgcatc aaagccatgc gccaaaaatt tgtcgagttg
     8461 ctcaaagcca aaggtgcaag ccaaaacttt gatttcatta tcgaacaaaa cggtatgttc
     8521 tctttcagcg gattgactcc cgaacaagtc gaccgtttga aaaacgagtt tgccatttat
     8581 gccgtccgct ccggccgcat caacgttgcc ggcattaccg acgacaacat cgattatctg
     8641 tgcgaaagca tcgtgaaagt cttgtaaaac aaaagcgcat acgaaatgcc gtctgaacct
     8701 taaccagttc agacggcatc tgttttttgt taaaaaacaa taaaccataa caatttcaaa
     8761 caagctaaaa acatctataa aatcaaccca atatgatgaa ctcaaataaa aaatacgaat
     8821 atcatcattc tcaaattcaa aatttgaacg ccacagtatt atccgaagcc acaaaaacct
     8881 ttccgccgtc attcccgcgc aggcgggaat ccgggaaccc aatgctgcaa gaattgatta
     8941 gaaacaactg aaaccgagca aactggattc ccgcctgtgc gggaatgacg aaataaggcg
     9001 agtaaaactg atgaaacaaa ttgattaaaa acaataactt atattatttt atttaaattt
     9061 tcgataaaat caattttccg tattcaatac tgctccaatc aattcccgcg caccgtcgtc
     9121 cgccaatttc ttagcgacag cgcgtccgag cgcgtcggca tattcggcag gggcttgcgc
     9181 gtccgcctgc aaaatgaccg aaccgtcggg gtgtccgacc agtccgcgca aggtcagcaa
     9241 gccgttttct tccgtgcaat atgcggccaa aggcacttgg cagcttccgc ccaaagcgcg
     9301 cgccaaagcg cgttcggccg taacgcaggc gtgtgtggta tcgtggttta gcggttttaa
     9361 aacctcatac aaatcttcgc ggtgcgcggc aatttcgata cccaatgcgc cttgtccggc
     9421 ggcaggcagg ctgtcggatt ccgacaaaat catgcggatg cgtccgtcca attccagacg
     9481 ctgcaaaccg gcggcagcca agataattgc gtcgtattcg ccgttatcga gtttagacaa
     9541 acgggtttgc acattgccgc gcaaaggttt gataagcaaa tgcggatagc gcgcacgcaa
     9601 ctgggcttcg cggcgcaggc tggatgtgcc tacgacggcg cctttgggca tttcttccaa
     9661 acgcgcgtat tggttggaca caaacgcgtc aaacggattg gcgcgttcgc cgatggctgc
     9721 aagcgcgaaa ccttcaggca aatccatcgg cacatcttta atcgaatgca cggccaagtc
     9781 agcgcgcccg tcatataaag cctgttccaa ctctttgaca aacaagccct taccgccgac
     9841 ttttgacaaa gttttgtcca aaatctggtc gccgcgcgtg gtcatgccca aaatctcgac
     9901 ttcgcaatcg ggatacaggg ctttcagacg gccttggata tgctttgcct gccacatggc
     9961 aagcaggctt tcgcgactgg cgataacgag ttttttcggg ttcataatgg ttgcctgaaa
    10021 gatggaaaac ggcatgaagt ctatcatatt ttgggcggtc tttcagacgg catgagggtg
    10081 aatatgttat atttacgcct aaaaaccaaa ttttccggac aacatgaaac gcgccaaaca
    10141 atatcctttt ttatccttac agcgacaacg ttttcatctg aactttgaaa acgcttcttc
    10201 caccgccaac cttccgagcg aacgcgattt ctaccgctgg gcgtggtctg ccttgaaaaa
    10261 tgaataccgc cgcgccgaca tcagcctgat tcttctggac gaagaagaag cccgcgctta
    10321 caaccgcgac taccgcggca aagattacgc caccaatgta ttgagtttcg cgctcaacga
    10381 aggcgaaatc ctgccctgcc aagtttcgga aaaactgtac ggcgatttga ttatctgccc
    10441 gcaagtggtt ttgaaagaag ccgccgaaca gggcaaaacg cccgaacggc attttgccca
    10501 cctgaccata cacggcgttt tgcacctgat gggctacgac cacatcgaag acgatgaagc
    10561 cgaaataatg gaagccgaag aaatccgcct gatgctggcg gcaggcttcc ccaaccccta
    10621 ccaagaggac gaatattaaa atggacggta cgcagtcgaa acccaagttt ttcgaacgcc
    10681 tgatctcccg acttgccggc gagcccgact ccgccgaaga cgtattgacc ctgctgcgcc
    10741 aagcgcacga gcaggaagtt tttgatgcgg atacgctttt aaggttggaa aaagtcctcg
    10801 atttttccga tttggaagtg cgcgatgcca tgattacgcg cagccgtatg aacgttttaa
    10861 aagaaaacga cagcatcgag cgcatcaccg cctacgtcat cgataccgcc cattcgcgct
    10921 tccccgtcat cggcgaagac aaagacgaag ttttgggcat tttgcacgcc aaagacctgc
    10981 tcaaatatat gttcaacccc gagcagttcc acctgaaatc cgtcttgcgc cctgccgttt
    11041 tcgttcccga aggcaaatct ttgaccgccc ttttaaaaga gttccgcgaa cagcgcaacc
    11101 acatggcgat tgttatcgac gaatacggcg gcacgtcggg tttggtcacc tttgaagaca
    11161 tcatcgagca aatcgtcggc gaaatcgaag atgagtttga cgaagacgac agtgccgata
    11221 atatccacgc cgtttcttcc gaacgctggc gcatccacgc ggctaccgaa atcgaagaca
    11281 tcaatacctt cttcggcaca gaatacagca gcgaagaagc cgataccatc ggcggcctgg
    11341 tcattcaaga gttagggcat ctgcccgtac gcggcgaaaa agtccttatc ggcggtttgc
    11401 agttcaccgt cgcccgcgcc gacaaccgcc gcctgcacac gctgatggcg acccgcgtga
    11461 agtaagcaga gcctgcccgc accgccgttt ctgcacagtt taggatgacg gtacggacgt
    11521 tttctgtttc aatccgcccc atccgccaaa cataaaaatg ccgtctgaaa ccggaaaaca
    11581 ggttcagacg gcatttcgct tatccgctta ttttttgctc aaagtcagcc ccgtccagcc
    11641 gaacagaagc gtcagtgcca aactcaaata gcagaaaaag gcatacggca gatattccca
    11701 caccggcaca cccagcgcgt ggctgataaa tacgccgcac acgctccacg gaacaagcgg
    11761 gttgatgacc gtacccgcgt cttcgagcgt acgcgagagg ttgcgggaat gtagtccgag
    11821 cctgtcgtaa acgggtttga acgtttctcc cgaaagcagg atgctcaaat attgttcgcc
    11881 aatcaggaaa ttgaccccga ccgaagtcat ggcaacactg aacgtcgcgc gtccggcatt
    11941 cgtcaaaaag ctgcggacgg catccagcag ggaaggaatc gcgcccagcg caaacagcag
    12001 tccacccaaa ctcatcccga gaatcacgat ggtttgcgtg aaaaacatac tttccaaacc
    12061 gccgcgcgaa atcagtttgg caacatcttt aaacgcttcg ccttcgagtt tgtaaccgcc
    12121 gtaaaaccac gcgccgagct gacgcagatc gggcgtgctg tgcagatacg ttacggcaac
    12181 ggcggcaatg acggtaaaga gcatggcgac cacggcattg acgcgcatca atgccaaaac
    12241 gaccaacagt gcaaacggaa tcagcgaata gccgtgcacc aatcccgtgg cttcaagctg
    12301 gctgcggaag gattcgacgc cgttcaaatc ctgcgcggcg acattcggca aaagccaaag
    12361 catcagtgcc gcactaatga gccacgcggg gatggtggtg tacatcatat ttttgatgtg
    12421 ctcaaacaag tcgatgccga cgatggacgc ggaaatgccc gtggtgtcgg aaagcgggga
    12481 cattttgtcg ccaaaaaatg cgcccgaaac aatcgcgccc gccgtcatcg ccatatcggc
    12541 atgaaacgcc gccgccatcc ccataaaggc aacgccgacg gtggcgcagg tggtcaggct
    12601 gctgccgatg gacacgccga tgacggaaca cagcgcgaaa gcggaaaaat aaaaataagt
    12661 cggggaaatc agcccgaaac cgtaatacat cagcgtcgga atcgcaccgc tcatcatcag
    12721 cgcgctgacc atcagcccga tgaagaaaaa caggtaaatc gcgcccatac cctgattcaa
    12781 cgcgcctatc atgccctgct gcatatcgtt gtatttcaaa ccgcgcgcca agccgtacaa
    12841 aatcagcacg acgatggcgg caataatgga catatgcggc agccactcca atgaaatgat
    12901 ggtatagccc atcgcggcaa tcagagcgac gacgacggca agtgcctcac cgcgcggcat
    12961 atcgagtaag gatttgaaag caaacatgat tctttgcccg atttgatata aagttaacgg
    13021 attctaacac accataccga atccgcccaa cgttttcaga cggcatttta gcgttttaca
    13081 aaagccggac agaaacaaaa cggcaatttt ctcaatttaa attaattttt tttgaaatat
    13141 aattttttat ttttttgttt gttggcatat aagactttta tggattgaaa tattaaaacc
    13201 tcggcacaca cgccgcaaac accggtacgc aaaaatgccg tctgaaagat gttcagacga
    13261 catttctcaa gaaaaagatt atttgtccgc cctatatccg tacaccgcaa aaaacacgat
    13321 atacagataa cagatgacgg aaacgacaaa cgaagacatc agggtgtaag tatctgccac
    13381 ccagccctgc acgacaggaa cgaccgcacc gccgacaatc gcggtacaca gtacaccgga
    13441 agccgcgttg gtaaattttc ccaatccttt ggttgccaaa gagaaaatcg tcgggaacat
    13501 aatcgagttg aaaaaaccga tggcaagcag cgaccacatc gccacatccg cattgccgct
    13561 acccgtcgcc atcgcgacgg caagcagtac gaccgcagcc gatgcgttaa acgccaaata
    13621 acggttgggc gcgaatttcg ccatcaccgc cgaaccgagg aaacgtccga ccatcgcgcc
    13681 gccccaatag aacgacaggt aatgcgcggc agaagcatga tccagccctt tcagataacc
    13741 caatacgttg accatcaacg aaccgataga cacctccgcg ccgacatagc agaaaatgcc
    13801 tgccgtaccg aacacgagat gtttgtattg ccatacgctg gttttgccgt cgtggttgtg
    13861 cgcgctttcc tcggcggcaa ttttgcgcgc gtcgggcagc cggatcattt tcacgaaaac
    13921 ggcaaggata atcagcagcc ccgccagtcc caaataggga atctgtacgg aagaaatctg
    13981 ttccgccttg ctgacggttt gggttgcgtc cgccaaaatc aggaacgcgc cgatttgcgg
    14041 cgcaatggtc gtacccaaag cgttaaacgc ctgaaccagc gtcagtgttg ccgattcctt
    14101 gccgggtttc gccagcaggg taacataagg attaccggca acctgaagca gcgttacgcc
    14161 ggaagccaaa ataaacaacg cgcccaaaaa taccgcgtaa gaatggctgc ccgcagcagg
    14221 ataaaacagc aggcatccga ccgccgtcag cagaaagccg ccgataacgc cgtttttgta
    14281 gccgactttg ccgacaaaag cccccatcgg gatggacatc accgcatagg cggtaaagaa
    14341 acagaattgg atcagcatcg cctgaacgta agacaggtcg aaaatttctt tcaaatgagg
    14401 gataaggatg tcgttcatgc aggtaataaa acccatcatg aagaacagcg tggtcaagac
    14461 gaccaatggg gacgtatgat tgttttgtga ttgcgcagac atgatttgcc tttcgtggtg
    14521 tcggaatatt acccgtttca agaaaataga aattgacatt tcttaacggc aatgcaaatc
    14581 atactgatat ttgaacattt tgcaaacttt atcggatgca ggcaatgcgc cgatctgaaa
    14641 ttcaagcagc agaaattaat attttacagt tgcttgcata gattttaata gattttaaaa
    14701 aattccggcg gacttgcaaa aaccgttccc aataaaatga aaatccgccg ccgtatccgg
    14761 aaaattcgga aatgccgcat cgtggcatca aaaccggagc ggaaagaatg tcgtatgccc
    14821 gacaaaaaag gggcatacgg ctgtgccgta ttttttgcac cgtcctccgc ccttcggcgg
    14881 gggcgtgaaa atatagtgga ttaacaaaaa tcaggacaag gcgac
//
LOCUS       DAXHMX010000039        14774 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHMX010000039 DAXHMX010000000
VERSION     DAXHMX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14774
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.039"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            43..675
                     /locus_tag="ACFM4S_000863"
     CDS             43..675
                     /locus_tag="ACFM4S_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6050581.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="ACFM4S_000864"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="ACFM4S_000864"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6050582.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQVQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2197..4065
                     /locus_tag="ACFM4S_000865"
     CDS             2197..4065
                     /locus_tag="ACFM4S_000865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6050583.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVEFSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(4217..4773)
                     /locus_tag="ACFM4S_000866"
                     /pseudo
     CDS             complement(4217..4773)
                     /locus_tag="ACFM4S_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            complement(4810..6960)
                     /gene="dinG"
                     /locus_tag="ACFM4S_000867"
     CDS             complement(4810..6960)
                     /gene="dinG"
                     /locus_tag="ACFM4S_000867"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6050584.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            7117..7287
                     /locus_tag="ACFM4S_000868"
     CDS             7117..7287
                     /locus_tag="ACFM4S_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050585.1"
                     /translation="MLLYRLLGGAEIECGRCPVPPMTDWQHFLPAMGTVSAWVAVIWA
                     YLIIESEKNGRY"
     gene            complement(7355..8656)
                     /locus_tag="ACFM4S_000869"
     CDS             complement(7355..8656)
                     /locus_tag="ACFM4S_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6050586.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(8646..9557)
                     /locus_tag="ACFM4S_000870"
     CDS             complement(8646..9557)
                     /locus_tag="ACFM4S_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6050587.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            9665..10063
                     /locus_tag="ACFM4S_000871"
     CDS             9665..10063
                     /locus_tag="ACFM4S_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6050588.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            10112..10717
                     /locus_tag="ACFM4S_000872"
     CDS             10112..10717
                     /locus_tag="ACFM4S_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6050589.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            10903..13014
                     /locus_tag="ACFM4S_000873"
                     /pseudo
     CDS             10903..13014
                     /locus_tag="ACFM4S_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            13212..13871
                     /locus_tag="ACFM4S_000874"
     CDS             13212..13871
                     /locus_tag="ACFM4S_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC6050590.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            14042..14737
                     /locus_tag="ACFM4S_000875"
     CDS             14042..14737
                     /locus_tag="ACFM4S_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6050591.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPAP
                     LTEGVNYTVLSTPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEASESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         3338 a         3812 c         4097 g         3527 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 atggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagtgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa cgtcgtttac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gtgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtt gcttatcccg atgtggattg ggaaacccac tgccgcaact
     1441 ttgtcgaaat cagcctcggt ctgaccttca acccctacac catccaaatc gaaccgcacg
     1501 actatatggc ggaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcgggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaaccccat cgactttgaa aactccgaag
     1681 gaaacctcgg tatggcaaac gccgtattgg gctttttgtc cgaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tcttacgcaa tatgggcgtg ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcacacctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggaactgct cgccgagccg attcaaaccg
     1921 tgatgcgccg ttacggtgtc gccaatcctt acgaaaaact gaaagacctg acgcgcggca
     1981 aaggcggcat tacgcccgaa gtgctgaaag gctttatcgg atcgctggaa atccccgtcg
     2041 aagccaaagc aaaattgctt gagctgaccc ccgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacggggcg atatttatgc aagctgtccg atacaggcct
     2221 gaaattgacg gattgcgggc cgttgccgtg ctatccgtca ttattttcca cctgaataac
     2281 cgctggctgc ccggagggtt tttgggggtg gacattttct ttgtcatctc gggattcctc
     2341 attaccggca tcattctttc tgaaatacag aacggttctt tttctttccg ggatttttat
     2401 acccgcagga ttaagcggat ttaccctgcc tttattgcgg ccgtgtcgct ggcttcggtg
     2461 attgcctctc aaatcttcct ttacgaagat ttcaaccaaa tgcggaaaac cgtggagttt
     2521 tctgcggttt tcttgtccaa tatttatctg gggtttcagc aggggtattt cgatttgagt
     2581 gccgacgaga accccgtact gcatatctgg tctttggcag tagaggaaca gtattacctc
     2641 ctgtatcctc ttttgctgat attttgctgc aaaaaaacaa aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcgggttt
     2761 tataccgata ttctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaaatggaa aacggcagtt gctttcatca ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ccgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcac tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgctt tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cgttgacggc cggattttcc ctgttgagtt attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcggaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgaggggg tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtaga tgagaagctg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcccg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt cccagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttgattaaag atattcccaa tgtgcattgg
     3901 gtggacgcac aaaaatacct gcccaaaaac acggtcgaaa tatacggccg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcttattata tggggcggga atttcacaaa
     4021 cacgaacgcc tgcttaaatc ttctcgcgac ggcgcattgc agtagcctgc cttgccgtcc
     4081 gatatcgttt gtgccgccgt ttgcctttcg gggcggcggc ggtttttatt ttccttcccc
     4141 tgcgggaggg aattttgaat caaaacccca attgcctttc caagttttcc accagattgt
     4201 catccagttc caaagcctac gacaggcggg cgaggaagac ggtttctttc cgcgacaaat
     4261 cggcacatac cagtcttgcc gccagatagg cctccgccgc caacgcctca tcgttgccga
     4321 cggcggcggc gatgtcttcg atgcttgcgg gcaggcggta ttcggcggcg agccatgcag
     4381 cagtttcggg gtctgtgccg ctttcctgtt cgatagtccg gcgttcggct tcgtctatca
     4441 tgccgtctga agcggcggcg gctatcatgg tgcgcaatac ggtgcggctg tatgtttctt
     4501 cggtttctcc ggcaggttgg aaatcgcttt gtgttacggt tgcccgccct ttgttttgct
     4561 gccacatctg atagccccgg taggcgaggt agcccaaagc ggcggtcgaa ccgattttgg
     4621 tgatggtttt gcggttttta ccgttcagca gcatggaggc gacaccggca accagcgcgc
     4681 ctccgccgaa tgaattgagc gggctgtcgg agaatgtgtt gccttttttt gaaccgtgct
     4741 taagacttgg ttgagcagtc gggtaaagtt catgaatttt tcctttctgt tgcgggaagg
     4801 gcggtatgtt taccctatcc ttttaaacgg cggcaggccg gccaataatt gttggccgta
     4861 ccgctgtgtt ttgatgcggt tgtcgaggat ggttacgcgg ccgtagtctt gttcggtgcg
     4921 gatgaggcgg ccgacggcct ggatgagttt gatgccggct tcggggacgg tgatttcaat
     4981 aaaaggattg ccgccgcgct gttctatcca gcggttttgg gttttttcga tggggttgtc
     5041 aggcatcgca aacggcagct tggcgatgat gacttgcacg caggcggtgc cgggcaggtc
     5101 gagtccttcg gcgaaactgt cgagtccgaa gatgatgctg gctttgcctt cttctatggc
     5161 ccggtggtgt ttttgcagga ggacggcttt gggtaattcg ccttgtacga gcaagagcgg
     5221 caggtagtct ccgggcaggc gcagggcgac atcctgcatt tgtttgcgcg aggaaaacaa
     5281 gacgagcgtg ccgatggctt cggtgggcga aataagcttg ggcagccatt cgatgacggc
     5341 ggcggtgtgg gcttcggggt ctttggggct ggcgtatatg ggggggatgt agagttcgcc
     5401 ctgtttttca aagtcaaagg ggcttttgag ggcgagggtg gtggtttcgg gcagccattg
     5461 cagcccggtt tggcgcagca tcaggttgaa gttgcccaag gattgcaggg tggcggaagt
     5521 caataccgcg cctgccgcac gccgccacag gctgttggca aggtgggatg cgctgctgat
     5581 ggggctggcg ttgaaaatgt agtcgttttt gtcgtcggca cggcgggtta tccattttgc
     5641 cagcggctct tcgccttcga gggggacggt ggagagcaaa tcccaaaccg cgctgatttg
     5701 ttcgatacgg acgatgaaga tgccgaactc gccggtcagg cggtcgagga gcgcgccgtc
     5761 ctgttctttt tcgcggcgcg cggcggaaag cgcatcgttc agcccgacaa cgtgtttgag
     5821 caggctgcgc gcagcaatgg ccgtattgga aacggtggtt tcgaggcctt cggggatttt
     5881 gccgtcttcc cacagccaag tcggttcgct gttggttcgt ctgtcgtttt cagacggccc
     5941 catacttaaa gacggctctt ccgccaaatg gaactgccat tcgtgcaggc tgtcgagcaa
     6001 ggatgcggcg gcttcgtcgg ccaggttggc aagttcggct ttatcggtaa gcgcggcaat
     6061 tttgccggtc agctgcggca gtttttccag cgtccaaacg gcaatattcc atgaatgttc
     6121 ggcggcaaaa cggctgaggg cttttttggg caggtggtgc gcttcgtcta tgcaatagaa
     6181 actgttttcg ggcgcaggca gaatcacgcc gccgcccata ctgatgtcgg caagcagaag
     6241 atcgtggttg gcaacgacga catcgacggt ttccaagaca tcgcgtgcta ggtaaaacgg
     6301 acattccgga cggttgggac aggcggtttt caggcagccg tggcggtcgt tggtcacttt
     6361 gagccaaatc gcgtcatcga ttttttccgg ccaagtgtcg cggtcgccgt tgaaccgtcg
     6421 ggcggaaaat tcgtcggcga tgtcgcgcag cagcttcaat tcttcgggct tgggtttgct
     6481 gtcccacaag acggcggggg cttcaaagcc gagcaggttt tgctgggcat tgctttgcgt
     6541 cagttgatag agtttgtagg ggcagagata gcggccgcgc cctttggcaa gcgcgaagct
     6601 cagttccaaa ccgctttttt cgaccaaaaa cggcaggtcg cggtctacca actgctcctg
     6661 caaggcaacc gttgcgctgc tcacaatcag ccgcttgccg cgcgtctgcg ccatgatgcc
     6721 gcccgccagc aggtaggcaa gcgatttgcc cacgccggtc ggcccttcga ttacggcgat
     6781 gctctcgccc tcgcgcttgg gcggctcgcc gccttcttcg cgcgccaacg tccgcgaaaa
     6841 agcgttggca accgccgcaa tcatttcccg ctgcgaagcg cgcggacgga aaccgggcag
     6901 gtttttgccg atgttttggt aatggtcgcg gatggcgttt ttttctaaat cggtgagcat
     6961 ggcgttttgt acggcggtag aagtgggctt attttaacat tgcacggaag cggtacaata
     7021 tcgttgtcgg aatggggggt gaggtgaatc gtgcggacgt ggttggtttt ttggttgcag
     7081 cgtttgaaat acccgttgtt gctttggatt gcggatatgt tgctgtaccg gttgttgggc
     7141 ggcgcggaaa tcgaatgcgg ccgttgccct gtgccgccga tgacggattg gcagcatttt
     7201 ttgccggcga tgggaacggt gtcggcttgg gtggcggtga tttgggcata tctgattatt
     7261 gaaagtgaaa aaaacggaag atattgagtc atccggacgc aatgccgtct gaaacggaag
     7321 ttcagacggc atttgtttta ggttgccgta ccgcttaggg aataccggcg acaggatagg
     7381 cgggatagcc gtgggtatcg accgaacagg caaaccgcca aggcgtgtgg acggtgtcgg
     7441 cggacaggtg ggcgagttcg ggaatgtgcc gtttgataaa agtaccgtcc gggtcggtac
     7501 ggtatgaggc ggcggcaatg tcggggcagg tgtgccgtga ggcggcaagc caccagttgc
     7561 cttggttgat tgccgcatcg aaatcggtca gctgtcgggc aaaccatatc tcgccttcgc
     7621 ggcgggggag gtttaaaacg tggcagaaaa aatccgcgct caagcgtctc agggcggggt
     7681 ggaggctgcc ggttttgtgc aaacagcgca tcgcggcatc gataatcgga atgccggtct
     7741 ggccctgctg ccaaagcgtc aggcgcaggg tgtgttcagg attgccgtct gaagggtcgt
     7801 catccgtgtg ctgcaaggca agttgaagga aaaaatcgcg gcggatgatg ttgtccgccc
     7861 acgcgttcag acggcgttcg aggctttccc gcgcgagcag gcgcggcgag atgcagccgg
     7921 cactcaaata cgcgcccatc agcgaagtgt gtttgcgcga ggggaaatcc tttaaaacgg
     7981 agtaggaatc cgcctgttcg agaaaccgcc gccactgccg ccaagccgcc gtttcgccgc
     8041 tgttttgcgg caggaagatg ccgtctgaaa gggcggcagg cggcggggcg gaaaggtttt
     8101 cggggaaggg ttggcggtat gccgcgaata ggtccggacc ggcggggggc tgcttggaaa
     8161 agcggtcgag ccatacttcg cggtagcggt cgaaatcggt gtgtgccgtg ccgccgtcgg
     8221 gtatcaggtc ggttttgccg aaaacggcgc ggtcattgac gaaggttaac gcgatgccgt
     8281 gtttgtccaa ttcatgccaa agggcgttgt cggcgagttt gtcggcaaaa gtatgggatt
     8341 cgtcggcgat gacggcgcgg atattgaggc ggacggcgag ccggacgagc tcggcaggcg
     8401 atgccgccgt gtagagcggg atgccgcgcc ctgcaagccc ttgggcgagt tcggcggcgg
     8461 attggcggta gaacgcggcg cggcgagggt tgtctgtttc ggcatcgtca atccaaatac
     8521 cgataacggg caaaccttcg gcaacggcgg tgcataaggc ggcgttgtcg cggatgcgga
     8581 ggttttggcg gaaccagacg agcgtgtgtg cggcgcacgt gtccgcataa agggggcggg
     8641 cggtttcaga cggcatttcg gcagcctttc ctgctggcga ttttttcgtt cagaaaatcg
     8701 atgaagctgc ggactttcgc gcttaagaat gccctgtctg cataaacggc attcagccgg
     8761 tcggtcggga cggcgtatcc gggcagcagc ctcaccagcg tgccgcagcg caaatcgtgt
     8821 tccgccgccc aaagcggctg ataaccgatg cacgcgcccg ccttaatcat ttcgcgcatc
     8881 atcagcgtgt tgtcggtacg gatgacgggg gtcagttcaa gccggtattt tttgccgtcc
     8941 gatttgcggg tgaggtcgag tttctgctgg ttggtgtagg tcggcaggac ggcgggcagc
     9001 cccgccactt cttccggcgt ttccggcacg ccgttgcgcc tcaggaagtc gggcgaggca
     9061 agcagggcaa attcgatttc cgccagtggg cgcgcaatca gtgacgggga cagggtttgg
     9121 gaaacgcgca acgccaaatc cacgccttcg gcaatcaaat cgacgtggcg gttgtccaaa
     9181 atcagttcta atgtcacttc gggataacgt tcgcggtatt ccgccagcca gttgcatatc
     9241 tggctgccgg caaaccacag tggcatcgtt acgcgcagca gcccctgcgg tttttccgtc
     9301 cccccggcgg ctttttgcgc ggcatcgtcg agcgtgtcga gcgcgtaact gcattgccgg
     9361 tagtattctt ccccggcttc ggtcaggctg aggttgcggc tgttgcggtg caggagtttg
     9421 gcttggacgg tgttttccaa gtggctgacg tgtttgcttg ccattgcggt ggagatgccg
     9481 agcgcgtcgg cggcgcgggt gaagccgccg ctttggacga cttggcggaa aaccttgagg
     9541 ctgaacaggg tgtccatatt ttcttgtgtg gaaaagttgt atcaataaaa gcagtatata
     9601 tttgaaaagg ggaaacatct atactctacc gcctgaaatg aagacaaata tcaaaggagc
     9661 ttttatgtcc gattgctgca accgtatcca accggttttg ctttctgttt tgcgtatcgt
     9721 aaccgcctac ctgtttttgt tgcacggtac gtcgaaaatc ttcgccttcc ccattgaaat
     9781 gggcagcggt tcgcccggcg ggctgttgct gcttgccggt attttagaaa ttgtcggcgg
     9841 cattttgctg gtgttgggcc tgtttgcgcg ccctgccgcg tttgttttgt ccggccagat
     9901 ggcggttgcc tattttatgg cgcacgcttc cggaaatgct ttgttcccga ttgccaacgg
     9961 cggcgagtcc gcagtgctgt tctgcttcgt attcctctat atcgcggcgg cgggcggcgg
    10021 agcatggtcg ctggacaggc tgtttttcaa gcgtaaagcc tgaatcggac tgcctaaagt
    10081 gtattttgtt gaatgttttt gaggaaaaga aatgacccgt caatctctgc aacaggctgc
    10141 cgaaagccgc cgttccattt attcgttaaa taaaaatctg cccgtcggca aagatgaagt
    10201 tgtccaaatc gtcgaacacg ccgttttgca cacaccttct tcgttcaatt cccaatctgc
    10261 ccgcgtggtc gtgctgtttg gcgaagagca tgataaggtg tggcaatttg tcgaagacgc
    10321 gctgcgtgcc gtcgtgcctg ccgacagttt tgaaccgacc gcgcaaaaat tgaacctgtt
    10381 taaggcgggt gcggcaacta ttttgtttta tgaagatcaa aatgttgtca aaggtttgca
    10441 ggagcagttc cctgcttatg ccgccaactt tcccgtttgg gcggaccagg cgaacgctat
    10501 ggtgcagtat gccgtttgga cgacacttgc cgcggtcggc gtaggtgcaa acctgcaaca
    10561 ttacaatccc ttgcccgatg cggcgattgc caaagcgtgg aatatccccg aaaactggtt
    10621 gttgcgcgca caaatggtta tcggcggtat tgaaggggcg gcaggtgaaa agacctttga
    10681 acccgttgca gaacgtttga aagtgttcgg cgcataattt cgcggtcaaa aaaatgccgt
    10741 ctgaaccctg ttcagacggc atttttcagt atcaggcggc gagttttccg cattctgaga
    10801 cctttgttta caaatatcat gttcaatata gttaaaagaa attattctca tttcctccgt
    10861 gaggcaatat aattcggttg ttttgttaaa ttgagtataa aaatgaaaat atcatttcat
    10921 ttagctttat tacccacgct gattattgct tccttccctg ttgctgccgc cgatacgcag
    10981 gacaatggtg aacattacac cgccactctg cccaccgttt ccgtggtcgg acagtccgac
    11041 accagcgtac tcaaaggcta catcaactac gacgaagccg ccgttacccg caacggacag
    11101 ctcatcaaag aaacgccgca aaccatcgat acgctcaata tccagaaaaa caaaaattac
    11161 ggtacgaacg atttgagttc catcctcgaa ggcaatgccg gcatcgacgc tgcctacgat
    11221 atgcgcggcg aaagcatttt cctgcgcggt tttcaagccg acgcatccga tatttaccgc
    11281 gacggcgtgc gcgaaagcgg acaagtgcgc cgcagtactg ccaacatcga gcgcgtggaa
    11341 atcctgaaag gcccgtcttc cgtgctttac ggccgcacca acggcggcgg cgtcatcaac
    11401 atggtcagca aatacgccaa cttcaaacaa agccgcaaca tcggtgcggt ttacggttag
    11461 tgggcaaacc gcagcctgaa tatggacatt aacgaagtgc tgaacaaaaa cgtcgccatc
    11521 cgtctcaccg gcgaagtcgg gcgcgccaat tcgttccgca gcggcataga cagcaaaaat
    11581 gtcatggttt cgcccagcat taccgtcaaa ctcgacaacg gcttgaagtg gacggggcaa
    11641 tacacctacg acaatgtgga gcgcacgccc gaccgcagtc cgaccaagtc cgtgtacgac
    11701 cgcttcggac tgccttaccg catggggttc gcccaccgga acgattttgt caaagacaag
    11761 ctgcaagttt ggcgttccga ccttgaatac gccttcaacg acaaatggcg tgcccaatgg
    11821 cagctcgccc accgcacggc ggcgcaggat tttgatcatt tctatgcagg cagcgaaaat
    11881 ggcaacttaa tcaaacgtaa ctacgcctgg cagcagactg acaacaaaac cctgtcgtcc
    11941 aatttcacgc taaacggcga ctacaccatc ggccgttttg aaaaccactt gaccgtaggc
    12001 atggattaca gccgcgaaca ccgcaacccg accttaggtt acaaccgcgc cttttccgcc
    12061 tccatcaacc cctacgaccg cgcaagctgg ccggcttcgg gcagattgca gcctattctg
    12121 acccaaaacc gccacaaagc cgactcctac ggcatctttg tgcaaaacat cttctccgcc
    12181 acgcccgatt tgaaattcgt cctcggcggc cgttacgaca aatacacctt taattccgaa
    12241 aacaaactca ccggcagcag ccgccaatac agcggacact cgttcagccc caacatcggc
    12301 gcagtgtgga acatcaatcc cgtccacaca ctttacgcct cgtataacaa aggcttcgcg
    12361 ccttatggcg gacgcggcgg ctatttgagc atcgatacgt tgtcttccgc cgtgttcaac
    12421 gccgaccccg agtacacccg ccaatacgaa accggcgtga aaagcagttg gctggacgac
    12481 cgcctcagca ctacgttgtc tgcctaccaa atcgaacgct tcaatatccg ctaccgcccc
    12541 gatccaaaaa acaaccctta tatttatgcg gttagcggca aacaccgttc gcgcggcgtg
    12601 gaattgtccg ccatcgggca aatcatccct aaaaaactct atctgcgcgg ttcgttgggc
    12661 gtgatgcagg cgaaagtcgt tgaagacaaa gaaaatcccg accgagtggg catccatttg
    12721 aataacacca gcaacgttac cggcaacctg tttttccgtt ataccccgac cgaaaacctc
    12781 tacggcgaaa tcggcgtaac cggtacaggc aaacgctacg gttacgactc aagaaataaa
    12841 gaagtgacta cgcttccagg ctttgcccga gttgatgcca tgcttggctg gaaccataaa
    12901 aatgttaacg ttacctttgc cgcagccaat ctgttcaatc aaaaatattg gcgttcggac
    12961 tctatgccgg gtaatccgcg cggctatact gcccgggtaa attaccgttt ctgatgaaat
    13021 caggcaaagg ccgtctgaaa atcctgataa tccgtaaatt cagcatacga actttttgac
    13081 aggctttatc tacatcagta taatccaaaa agaaatagtg cccttggcag tacgcattac
    13141 ccattaaatc atagcgggta tgccgatccc aagggctttt ttatatcttt gacaaatata
    13201 ggggaagcaa tatgaaaacc gccattttga ttgacggtgc attttttatt aagcgggtac
    13261 ggtcattagg cctgtctcaa aatccacacg atgcaaaaag aattgctgat ttggctttcc
    13321 gatattcgat gctgcatctg aaagataaaa tcaacggcac agatcaaatg gatgatttat
    13381 atagaatttt cttttacgac tgcgtgccgt ttgaaaagaa aatgcacaat cccgtatctc
    13441 atcgtgccat agatttttca aagactccgg aagccatatt tcgttgcaat ctgcataccg
    13501 aattgaagaa gaagcgtaaa ttagcgttac gtttaggcaa gctgtcggac aatacagcat
    13561 ggatattaaa accccaagtc atgaaaaatc ttctgaaaaa cccgtcaact caaattacgg
    13621 aaaacgatgt cgtgctcgat gttaaacaaa aaggtgtaga tatgcgtata ggcttggata
    13681 tttcatctat taccttaaaa aaacaagccg ataaaatcat cttgttttct ggtgattccg
    13741 attttgtccc agcagccaaa ttagccagac gggaaggtat cgattttatt cttgatccta
    13801 tgtggaatca tgtcccacct gacttaatgg aacacatcga cggattgaag acaaaatgtc
    13861 cgaaaccgta aattactaga acaaaggccg tctgaaaata aggctaatta tctaagccat
    13921 atttcagacg gcctttataa aggctgaaat aaaactaaac acattttttc actcaaatcg
    13981 aacacgcctt caataaaatg tcataaaatc cgcacattaa tctgacacac aagagatacc
    14041 tatgaaactg aaaaccttag ctttgacttc attgaccctg ttggcattgg ccgcttgtag
    14101 caaacaggct gaaaccagcg ttccggcaga cagcgtccaa agcagctcat ctgctccggc
    14161 agccccagcc ccattgaccg aaggcgtgaa ctacactgta ttgtccacgc ctatcccgca
    14221 acagcaggcc ggcaaagtcg aagtcttgga atttttcggc tacttctgcc cgcattgcgc
    14281 ccatcttgag ccggtcttga gcgagcacat caaaacgttt aaagacgata cctatatgcg
    14341 ccgggagcat gtcgtgtggg gtgatgaaat gaaacctttg gcacgtttgg cggccgcagt
    14401 ggaaatggcc ggtgaatcag ataaagccaa cagccatatt ttcgatgcga tggttaatca
    14461 aaaaatcaat ctggccgata ccgataccct gaaaaaatgg ctgtccgagc aaacagcgtt
    14521 tgacggcaaa aaagtattgg ctgcatttga ggcttctgaa agccaagcgc gtgcggctca
    14581 aatggaagag ttgaccaata aattccaaat cagcggcaca ccgactgtga tcgtcggcgg
    14641 caaataccaa gttgaattta aagactggca gtccggtatg accacgattg accagttggt
    14701 ggataaagta cgcgaagagc agaaaaagcc gcaataagtt gaggattgaa tgagtaaagg
    14761 ccgtttgaaa atag
//
LOCUS       DAXHMX010000040        14576 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHMX010000040 DAXHMX010000000
VERSION     DAXHMX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.040"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(84..737)
                     /locus_tag="ACFM4S_000876"
     CDS             complement(84..737)
                     /locus_tag="ACFM4S_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6050592.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
     gene            complement(769..1281)
                     /gene="coaD"
                     /locus_tag="ACFM4S_000877"
     CDS             complement(769..1281)
                     /gene="coaD"
                     /locus_tag="ACFM4S_000877"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6050593.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            complement(1405..2088)
                     /locus_tag="ACFM4S_000878"
     CDS             complement(1405..2088)
                     /locus_tag="ACFM4S_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6050594.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            complement(2245..2511)
                     /locus_tag="ACFM4S_000879"
     CDS             complement(2245..2511)
                     /locus_tag="ACFM4S_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6050595.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(2584..3054)
                     /gene="rlmH"
                     /locus_tag="ACFM4S_000880"
     CDS             complement(2584..3054)
                     /gene="rlmH"
                     /locus_tag="ACFM4S_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6050596.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3108..3494)
                     /gene="rsfS"
                     /locus_tag="ACFM4S_000881"
     CDS             complement(3108..3494)
                     /gene="rsfS"
                     /locus_tag="ACFM4S_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6050597.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(3553..4158)
                     /gene="nadD"
                     /locus_tag="ACFM4S_000882"
     CDS             complement(3553..4158)
                     /gene="nadD"
                     /locus_tag="ACFM4S_000882"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6050598.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRRGETYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            complement(4155..4754)
                     /locus_tag="ACFM4S_000883"
     CDS             complement(4155..4754)
                     /locus_tag="ACFM4S_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6050599.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(4783..6324)
                     /locus_tag="ACFM4S_000884"
     CDS             complement(4783..6324)
                     /locus_tag="ACFM4S_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6050600.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAEVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGK
                     QTETL"
     gene            6563..7948
                     /locus_tag="ACFM4S_000885"
     CDS             6563..7948
                     /locus_tag="ACFM4S_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6050601.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            7968..8486
                     /locus_tag="ACFM4S_000886"
     CDS             7968..8486
                     /locus_tag="ACFM4S_000886"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6050602.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(8825..9742)
                     /gene="thrB"
                     /locus_tag="ACFM4S_000887"
     CDS             complement(8825..9742)
                     /gene="thrB"
                     /locus_tag="ACFM4S_000887"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6050603.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(9779..10507)
                     /gene="ubiG"
                     /locus_tag="ACFM4S_000888"
     CDS             complement(9779..10507)
                     /gene="ubiG"
                     /locus_tag="ACFM4S_000888"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6050604.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            10659..11900
                     /locus_tag="ACFM4S_000889"
     CDS             10659..11900
                     /locus_tag="ACFM4S_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6050605.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            12033..12596
                     /gene="gmhB"
                     /locus_tag="ACFM4S_000890"
     CDS             12033..12596
                     /gene="gmhB"
                     /locus_tag="ACFM4S_000890"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6050606.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            12626..13369
                     /locus_tag="ACFM4S_000891"
     CDS             12626..13369
                     /locus_tag="ACFM4S_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6050607.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETV"
     gene            13366..14055
                     /locus_tag="ACFM4S_000892"
     CDS             13366..14055
                     /locus_tag="ACFM4S_000892"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6050608.1"
                     /translation="MTSLIHTLSDGMELTIEIKRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPQTAENRLPESILFHGRQLALTAHQDTQILLMPS
                     EIRVPEGAPEKQLALLQDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQWL
                     KIHGRELFALG"
     gene            14167..14352
                     /locus_tag="ACFM4S_000893"
     CDS             14167..14352
                     /locus_tag="ACFM4S_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050609.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRDP
                     PGPTCGMKRLSWREVFY"
BASE COUNT         3338 a         4445 c         3697 g         3096 t
ORIGIN      
        1 tgaagagatt tgcagtcaac cgttttcttg aaaaattttt ataaaaatat ttatcatatt
       61 gtttataaat taatttatat ttattaacct gcttttgcca tataaaattc ttccagcgca
      121 tgagagatat tttcagtctg ccattcgttt tttaagggtg caacaatttc gatttgtcgg
      181 ttttggtagg caaattgtat tttccatgca tacagaaaca tggtttcgga ttctgttccg
      241 ccgtataagc tgtcgcccag aatcggactg cccaaacttt tcatcgccac tctcaattgg
      301 tgcgttttgc ccgtatgcgg ttccaggatg aacagccgca gtttttcggc gatactgatg
      361 ctgtggaatc gggtaacggc gatattttct gtattgcgcg tcaacttcca cattccgcgt
      421 ctggattttt ccattccgcc tttaatccaa ccctgctttt tggacggttt gcggtcggat
      481 agtgccaaat aggttttttt gatgcttttg ccggcaaact gtccgctaag ggcggacgcg
      541 ctttctctgt tgagggcaaa cagtaaaatg ccgctggtct gtttgtccaa tcggtgcagc
      601 agccacacac gctctacgcc caactgtatg gcgagtgttc gggccagtcc ggtctcgccg
      661 ctgtcttggt ggacggatat gccgcccggt ttgttgatgg cgacgaagtc ttgatggcgg
      721 aacaaaattt ccaacatatc catatatgcc ttgcaaaaat agaagggttc aattttcgtg
      781 ttgatgttcg gcaaggattt tttcgtacac agcttgcggc acgtagcggt ggatcgtttc
      841 cgtccagcct tccggcccga ccagtccttt gaccatagtg gacgacactt cggcgatttc
      901 gcgcggcggc atgaggaata cggtggatat ttcgggggcg aggtcgctgt tgatatggcg
      961 catggaacgt tcgtattcgt aatccgaagc agaacggatg ccgcgcacga tgaatcctgc
     1021 atctacctca cgggcgtaat gcaccagaaa tcggttttca aatacatcgg ttctgacgtt
     1081 gggaaacatt ttagtaatat cgcacaacat atcctgcctt tcagcgacgg tataggtgct
     1141 gcgtttgtcg gggttaatgc cgatggcgac gatgagttcg tcaaacatag attgcgcctg
     1201 ccgtatcatc cacagatgcc ccaatgtggg cggatcgaaa ctgccggcat aaacggcgcg
     1261 gcgcggtgta ttcggtaaca tttgattcct cccggcttca tagtcggctg tgtgttggtg
     1321 cgtgtgcatc cgtattgtat gcccaaagta aaatgccgtc tgaagcattt tcagacggca
     1381 tagtcggacg gcgttttgcc ggcatcaatc ctcgccgttt aaagacaaca ggatgttcag
     1441 caggctgctg aagatgttgt aaagcgagat aaacagtgtc agtgccgcgc tgatgtggct
     1501 gtcttcgccg ccgtcgatga cggtgcgtac ctgccacata atcattaagg aactgaacaa
     1561 gacaaaaccg gcggaaatgg tcagggcgag tgcgggaata cccaaaaaca gattggcaac
     1621 cacggcgacc atcagaatga ccgcacctac ggtcaggaag cgtccgagcg cgttcatatc
     1681 gagccgggtt cggcgcgcca aggcggacat cgttaaaaag acggcggcgg tcatcgcggc
     1741 ggcaatgccg acgattttcg caccgtcggc aatatggagc gcgtattgca gcacggggcc
     1801 gatcaatacg cccataccga atgtgaatac catcagcagg gtaacgccgg tattgctgta
     1861 acggtttttc tcgatgaagt ggatcatacc gtagaaaaac gccaacacga cggcaaaccc
     1921 tatccagcgc gaaccgaagg cggcgtaaaa attgaaaccg gcattggcgg caagtgccgc
     1981 gcctgcggct gccggaatga atgaaaatcc gagcaggcgg taggttttct gcaggacggt
     2041 gtttttagaa accgtatgcg cggtgtagtc gtaaacgtcg tgttgcatat catctgctcc
     2101 tgaaagcgcg gttgggaata atgggggatt ttaacattgc ccaatgtcaa aatttgtccg
     2161 gttgcgtgaa gataaagttg tccagcgtat tttaaaggcc gtctgaagca gtttcggaca
     2221 gcctgtgttc aaaacggaaa accgttattg cggaacgtat ccctgaacgg catccgcgcc
     2281 gtcgccgaag aaatactgct ccatctgctg agccaggtat tcgcgcgcgc gcggatcggc
     2341 aaggcttaag cggttttcgt taatcagcat ggtttggtgg cgcgtccacg ccgcccacgc
     2401 ttcttgcgat acgttttcaa aaatgcgctt gcccaattcg ttgggaagcg gcggaaattt
     2461 catgccttcg gcttctttgt tgagcttgac gcagaatacc atgcgtgcca tagcggattc
     2521 ctttgctgtg ttcagaaata acggggtgat tttaaccgat taggggggcg gacaaaagcc
     2581 ttcttattcc cgatgataag gatggttgtg caggatggaa acggcccggt agagctgctc
     2641 ggtcagaagg acgcgcacca tgccgtgcgg cagggtgagg ctggacaggc gcatcatcat
     2701 gcgggcttgt tgtttgaggc ggtcggtcat gccgtccgcg ccgccgatga cgaagcagac
     2761 gtgttcgccg ttttgccgcc agcttttgag gtgttccgcc agctcgacgg aggtcggcgc
     2821 tttgccgcgt tcgtcaagaa cgacaaggaa cgcgccttgc ggaacggctt caaggatgcg
     2881 tttttcttcc gccgccatac cttgggcggc attcacgccc gcgccgcgtt tttcgggttt
     2941 gatttctttg agtgcgtagg cgacgtcgcg tccgaagcgt ttggcgtatt cggcgacggc
     3001 ctcatcaacc cagcgcggca ttttggtgcc gactgccaaa acggtaatgt tcaatgcttt
     3061 ctcccttaca ggaaaatgcc gtctgaacat tcagacggca tcgggaatca gtctgccgcg
     3121 tgccacggct tctgcattcc ggcgtggaaa ctcggtttct cgccgcccca aagggtgtcg
     3181 atgtcgtaga agtcgcgcac ggcagggagc ataacgtgga caacgaggtc tcctgcatca
     3241 accaacgtcc attcgccgct gtcgccttcg gtactgagga tttcaaaacc ggcttctttc
     3301 aaatcgacgg caacgttgtt ggccagtgct ttgacttggc gcgtactgtc gccgctggcg
     3361 ataatcattc tggcaaacag cgaagtttta tcttgcgttt cgagaacgga aatgtctttg
     3421 gctttgatgt cttcgagggc gttgacggcg accccgacca ttttttgcag gtcttgcagt
     3481 tcttgttcgt tcattatgtt cctaacggga tgttttcaga cggcattata gccgtttctt
     3541 actgatttga ctttattttt catacaaacc gtgttcgcgg atgtagcgtg cggcggcagg
     3601 cgggatgccg tctgaaacgc cttggccggc aaggttgcgg cggatttccg ttgacgacac
     3661 attatgcatc ggggcggaca agatgcggac gctgccgtcc tgaagcgcat tgcccagcca
     3721 cgcgtgcagt tcgcgcgggg tttggtgcag gctgtcgccc tgccgcatgg cgacggcgat
     3781 attggtttcg cgcacgagca tctgccattt tttccatgtg tgcagcttca tcaggctgtc
     3841 gctgcccatc agccaccaga gttgcgccga ggggaactgc tggcggaaaa tttggacggt
     3901 gtcgaaagtg taagtctcgc cccggcggac gatgtcgcag tcgctgacgg caaaacgcgc
     3961 atcttctgcc gtcgccaatt cgaccatggc aaggcggtcg gcggcggaag cggaggcggc
     4021 gtctttgtga tacgggccgc ctgtcggcag gaaaacaacc gcgtccaagc cgatttcgtc
     4081 ggcaaaggcg cgggcgatgt ggagatgccc gttgtgtatc gggtcgaaag tgccgccgaa
     4141 cagtccgatt ttcttcatga tgtttccatt ccttccgata agtccatgcc gtctgaaaca
     4201 ctgccgtcca caaccagcgt cagtttgccg gtaacgggat ggatgaccag tccgtgaacg
     4261 gcgatatggc gcggcatcag cggatggtta cggataaggt ccaccgtgtg gcgcacactg
     4321 tcttcgacgt tgtcgaaacc ggtcagccag ccgtcgaggt cgataccggc ataacgcagg
     4381 gtttcgatac ggtcttcggg aatctggctt tcccggacgc gcccgaggaa ttcttcggca
     4441 ttcagcccct gcataccgca atcgtgatgg gcgatgacca taatctctct gaccttcaat
     4501 tcaaacacgg caaccaaaag actccgcatc accgaacccc acgggtgcgt aaccagcgcg
     4561 ccggcatttt taatcagctt ggcatcgccg tttttcaaac ccaacgcgtc gggcagcagc
     4621 ccgataatcc gcgcatccat acaggacaaa actgccagcc cgcgttcggg gtatttgtcg
     4681 gtaaagtatt tttcatattc gcccgactcg acaaactgcc ggttatgggc aaggatgtta
     4741 tccaactcgc tcattttgcc gtcctctgaa aaagggttca cattataacg tttccgtctg
     4801 ttttccgcct tcgccgttgt ccaacagcag gaaaatgccc agcgcgaacg ccgacaacag
     4861 caatgtcaac accattgccc gcgcataatt atcctcaccc gcacgcccca aataggcata
     4921 aatcaaagtc gtcagcgtct gccactcggg acgcgacagg aacaaggttg ccgcaaattc
     4981 gcccacgcag gttgccgccg ccaaagtcag accgcgccgc aacgccggtt tcaagagggg
     5041 gaacgtgatg cggcatgccg tctgaaagcc gtttgcgccc aaacccgccg ccgccctgcc
     5101 gtaatccggc ggcagtgcgt cccaagccga caaaacatct tttgccacaa acggatacgc
     5161 cagcagcgca tacatcgcca gcagcaacgg caacgaagcc gtccactgcg gataaagcag
     5221 cagcacgccc gccgaaacac aaaccggcga caccataaac ggcaaaaaca tcagcccgcg
     5281 catccacgcc gaccgccgcg ccgccgccgc atacaccaca cccaaaaccg ccgccgcaaa
     5341 caccgccgcc gccgagaagc gcaaagtatt ccacaccgcc tgccacgttt cactttccat
     5401 taacacacgc cacgattcgc cggccgacca cgctttcaca acaattgcca acaaaggaaa
     5461 caggcagcac acagacaaca cctccgccgc aaacgccagc agcacatatt ccccgaccga
     5521 ctgcggcggc gacggcatca caggggaaac cgccttatcc gaaaccgcgc gcctgccgaa
     5581 ccacgcatac agcaaccctg ccgccgccgt tacccccaac accagccaca ccagcaccga
     5641 agcaaccgcc atatcgagtt cgaacatgac caactggtaa atttccactt cgaccgtggc
     5701 ataacggctg ccgcccagca gcagcgccag cccgaacccg gaaaaacaat acagaaagac
     5761 aaggcacacg ccgccggcaa gccacgggcg caaaacgggc atttcaatgt cccaaaaccg
     5821 ccgccacgcc cccgcgccca acgtccgtgc cgtctgaagc cgtgccgcag gcacttgcac
     5881 aaacccctga tacgccgccc tgaccaacac aggaaggttg aaaaacacat tgccgtacaa
     5941 caacagatac ggcgtatcct gccgtccgcg ccacaacagg ccgtccgccc caaacagagc
     6001 cagcacgccc acgcccgcca ccaacgtggg catcacaaaa ggcagcatca gcaggcgcag
     6061 caccaaagcc cgccccggaa acgccagccg cgccagcacc cacgcgacag gcacgcccaa
     6121 aggcagcacc agcacacagg ttgccgctgc ctgaaatacc gtccacgcca aacgtttgag
     6181 catataggca tccgacagca ccgcgcgcca cgccaaaccg tcatacgccg ccaccgccca
     6241 caaaggcgca acgaccatta ccgccaaaaa agccgaaggc agcagggcaa aagcacccca
     6301 taccgcccaa cgccgtccat ccatcgcctt ccccacttga aacactgatg ttgcgattgt
     6361 acccaaaagc cccacatacc gtatatttca atccgactac ataccgtatc cgccttcctc
     6421 ccgccgtctg aaataaatcg ttcaaataaa caggaatata acttcagaca aacaacttac
     6481 cgcccgattt gtgctatcgt tttcgcacaa cttaaaaaaa cctgacaatt ttgtactttt
     6541 attacagaga aaggctttac aaatggacgg ctggacacag acgctgtccg cgcaaaccct
     6601 gttgggcatt tcggcggcgg caatcatcct cattctgatt ttaatcgtca aattccgcat
     6661 ccacgcgctg ctgacactgg tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc
     6721 cacaggcagc attgtcaacg acgtactggt caaaaacttc ggcggcacgc tcggcggcgt
     6781 ggcgcttctg gtcggcctgg gcgcgatgct cggacgtttg gtcgaaacat ccggcggcgc
     6841 acagtcgctg gcggacgcgc tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct
     6901 gggcgttgcc tcgctgattt tcggcttccc gattttcttc gatgccggac taatcgtcat
     6961 gctgcccatc gtgttcgcca ccgcacggcg catgaaacag gacgtactgc ccttcgcgct
     7021 tgcctccatc ggcgcatttt ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat
     7081 tgccgcttcc gaattttacg gcgcgaacat cggccaagtt ttgattttgg gtctgccgac
     7141 cgccttcatc acatggtatt tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat
     7201 ccatgttccc gttcccgaac tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga
     7261 acctgccaaa gcaggaacgg tcgtcgccat catgctgatt cccatgctgc tgattttcct
     7321 gaataccggc gtatcggccc tcatcagcga aaaactcgta agtgcggacg aaacctgggt
     7381 tcagacggca aaaataatcg gttcgacacc gatcgccctt ctgatttccg tattggtcgc
     7441 actgtttgtc ttgggacgca aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga
     7501 cggcgcactc gcccccgtct gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg
     7561 cggcgttttg cgcgcttccg gcatcggcaa ggcactcgcc gacagcatgg cggatttggg
     7621 cattcccgtc cttttgggct gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc
     7681 ggcaaccgtc gccctgacca ccgccgccgc gctgatggct cctgccgttg ccgccgccgg
     7741 ctttaccgac tggcagctcg cctgtatcgt attggcaacg gcggcaggtt cggtcggttg
     7801 cagccacttc aacgactccg gcttctggct ggtcggccgc ctcttggaca tggacgtacc
     7861 gaccacgctg aaaacctgga cggtcaacca aaccctcatc gcactcatcg gctttgcctt
     7921 gtccgcactg ctgttcgcca tcgtctgaca gacggaaagg atagtaaatg actacgcatt
     7981 ttgtcgttat gggcgtatgc ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac
     8041 acctcggtca atgtccctat gccgaaggcg acgagttcca cacccaagcc aaccgcgaca
     8101 agatgggcgc gggtattccg ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc
     8161 gcgactggat gacgcaacag gcgcaaaacg gtgcgaacca caccatcgta acctgttccg
     8221 ccctcaaacg cggctaccgc gacattctgc gcggagccga aggcaaagct gccttcatcc
     8281 acctcagtcc gccgcaagac atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca
     8341 tgaaagcagg gatgctcgat tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat
     8401 acggggtcaa aatcgccaac cccggcacgc ccgaagcggt cgaagccgat attctgaact
     8461 gggttgcctc ggaaaacctg ctttgaagca atatgccgtc tgaagcccga cacaggatgg
     8521 gtttcagacg gcataaacat cgggaacaga atggattaca ttgatttata gtggattaac
     8581 aaaaaccagt acagcgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga
     8641 agcaccgagt gaatcggttc cgtactatct gtactgtctg cggcttcgtc gccttgtcct
     8701 gatttttgtt aatccactat aaaatggaaa atacccggct atcgtctcat tttcgtttta
     8761 atcagccata aaaatgccgt ctgaaaccct ttcagacggc atttctgtca aacgccggac
     8821 gcactcaatc caaactcaac agcaggttgc ggaacgcgtt cgggtctttg ataaacgtca
     8881 tctcgcccgc ctgcggaaaa tgaaagtcca acaggcgcga cacccaaaaa cggatgcagc
     8941 cggcacgttg ggcggtcggg aaatacgcct tttcttcggc actcaagggg cgcacgccct
     9001 cataaccgcc gataaacacc tttttcaacg cctcatccaa cttattgtcc gccgtccttg
     9061 cccaatcgtt gaccgcaatc gccaagtcat acataaaatt gccccggcag gcataataga
     9121 aatcgatgaa acccgatacc tgaccgccgt caagcaacac attgtcttta aacaaatcgg
     9181 catggatgat gcccgaaggc agatgattgc cgagattgtc ctttaacgca tcgatttcgg
     9241 aacacagcag cgcggcatcg tcttgcgaca ggacgggcag cagccgggtg cacgcctccg
     9301 tccaccacgc atcgtaacgc gggttttcca tttccaaagg gaaatcggcg gcggcaaggt
     9361 gcattttggc cagcatcgca ccggtatgga aacactgctc cgccgtcggc agcgcggtat
     9421 ccgaaccttt caggcaggca accaaacaag ccggcttgcc cgccaaaacg gaatcaagcc
     9481 ggccgtcttt gcgcgcaacc ggcgcggcaa ccgccacgcc cttcatactc aaatgccggt
     9541 taagctccag aaaaaacggc agctcttcct gtttcaacac ttcaaacacg gtcagcacat
     9601 aacgtcccga agtcgtcgtc agaaaataat tgctgttggt aatcccctgc gcgatgccct
     9661 gcagggaaac aaattccccc aaatcgtaac cgctcaggaa gccgcgcatt tcatcatcgg
     9721 aaacactggt atagacagac atatcaactc acttgctcaa aaacgggcaa acccgccgtc
     9781 agaacgccgg acggcaggca aacatataat tcacatcggt cgaatcgcac agggcataag
     9841 tttgcgacaa cacatggtaa gtcataccct tcgtatccgc cacatccaag cctgcctgac
     9901 ggcacattcg cgccagctcg gcaggtgcga tgaatttttt ccagtcgtgc gtgcctttgg
     9961 ggacaaactt caacagatat tccgccgcca caatcagatg caggtacgat ttcgggtttt
    10021 tattgatggt ggaaaaaaac accatgccgt ccggcttgac cagcttggca caagcacgca
    10081 cgatggcggc gggatcgggg acgtgttcca tcatttccat gcacgttacc acatcgaacg
    10141 agtgcggttc cgcctcggca aggtcttcca cgcggataca ttcgtattcg atatcggcga
    10201 cattgttcaa agccgcgtgc aggcgggcgg tttccaacga ctgctccgcc atgtcgatgc
    10261 cctttacaaa cgccgcgccg cgccgtgcca tactttccgc caagatgccg ccgccgcagc
    10321 ccacgtccaa aacccgtttg ccgcacaaat ccgcgtgccc gtcgatataa tccagccgca
    10381 gcggattgat gtcgtgcaag gttttgaact cgcccgactt gtcccaccat ttgtcggcaa
    10441 tccggctgaa tttggcgatt tccccctcat cgacattata ttttttgtcg gacattttcc
    10501 ctcccatctg acgaaccgcc cactccaaaa cccaagatta tactatggaa cacaatgccg
    10561 tctgaaagcc ttttcgggcg gcgcggcgca aatgccttac aaatccttac actttacggc
    10621 ataatggcgg ctcgcttttt ctggcagaaa gacaaaatat gcccaccaaa accccttcac
    10681 tgttcggcgg cgcgatgatt atcgccggca cggtcatcgg cgcaggtatg ctcgccaacc
    10741 cgaccgccac atccggcgta tggtttaccg gctcgctggc cgtgttgctg tacacctggt
    10801 tttccatgct ctccagcggc ctgatgattt tggaagtcaa cacccactac ccccacggcg
    10861 cgagcttcga caccatggtt aaagacctgc tcggacggag ctggaacatc atcaacggca
    10921 tcgccgtcgc cttcgtttta tacctgctta cttacgctta tatcttcgtc ggcggcgacc
    10981 tgaccgccaa aggcttaggc agcgcggcag gcggcaatgt ttcactcacc gtcggacaac
    11041 tcgtcttctt cggcattctc gccttttgcg tatgggcatc cgcacgcttg gtcgaccgat
    11101 tcaccagcgt cctcatcggc ggcatggtat taacctttat ttgggcaacc ggcggcctga
    11161 ttgccgatgc caaactgccc gtcctcttcg acacccaagc ccctaccggc accaactact
    11221 ggatttatgt cgccaccgcc ctgcccgtct gccttgcgtc attcggtttc cacggcaacg
    11281 tctccagcct gctcaaatac tttaaaggcg acgcgcccaa agtggctaaa tccatctgga
    11341 cgggcacact gattgcgctg gtaatttacg tcctctggca aaccgccatc caaggcaacc
    11401 tgccgcgcaa cgagttcgcc cccgtgattg ccgccgaagg gcaagtctcc gtcctgattg
    11461 aaaccctgtc caaattcgcc caaaccggca atatggacaa aatattgtcc ctgttttcct
    11521 atatggcgat cgccacctcg tttttaggcg taacgctcgg actcttcgac tacatcgccg
    11581 acatcttcaa atggaacgac agcgtgtccg gccgcaccaa aaccgccgcg ctgaccttcc
    11641 tgccgcctct aatttcctgc ctgctcttcc ccaccggctt tgttaccgcc atcggctacg
    11701 tcggcctggc ggcaaccgtc tggacaggca tcatccccgc catgctgctc taccgttcgc
    11761 gcaaaaaatt cggcgcaggc aaaacctata aagtttacgg cggcttgtgg ctgatggttt
    11821 gggtcttcct tttcggcatc gtcaacatcg ccgcacaggt attgagccaa atggaactcg
    11881 tccccgtatt taaaggataa aggcaaaatg ccgtctgaag cccgccggcg gcttcagacg
    11941 gcattgcctc gttatcacgc ggcacaggcg catcactgcc gatatttcag tataatgcca
    12001 cccccgacct gccccaatcc aaaggaaacg cgatgaaact catcattctc gaccgcgacg
    12061 gcgtcatcaa tcaggaccgc gatgacttcg tcaaatccgt tgacgagtgg atacctgtcg
    12121 aaggcagcat ggatgcggtg gcatttctga cgcaggcagg ctacaccgtc gccgttgcca
    12181 ccaaccaatc cggcatcggg cgcaaatatt ttaccgttca aaaccttacc gaaatgcacg
    12241 ccaaaatgca ccgcctcgtc cgtcaggcag gcggcgaaat caacggcatc tggttctgcc
    12301 cgcacaccga tgccgacaac tgcaactgcc gcaagcccaa accgggtatg attgaagaca
    12361 tcatcggacg cttcaacgcc caagcctcgg aaacctggct ggtcggcgac agcctgcgcg
    12421 atttgcaggc aatcgatgcc gtcggcggaa aacccgcgct ggttctgacc ggaaaaggca
    12481 aaaaaacgct ctcccaacac ggacacgaat tacccgaaca cacacaggtt ttcgatacct
    12541 tgctcgattt ctcacaatac atcatgcagg aaaacgccgc accgcaagcc gactgaacat
    12601 accgcatttc cgacaaggca aaaccatgct catcatccgc aacctgattt actggctgat
    12661 actctgttcc accctgattt tcctctttcc ctttatgctg ctcgcctcgc ctttccggga
    12721 cggggcgcac aagatggcgc gggtctgggt caaaatcctc aacctctcgc tcaaacacat
    12781 cgtcgggctc aaataccgca tcatcggcgc ggaaaacatc cccgaccgcc ccgccgtcat
    12841 ctgcgccaaa caccaaagcg gctgggaaac gcttgccctt caggacattt ttccgccgca
    12901 ggtttacgtt gccaaacgcg agctgttcaa aatccccttt ttcggctggg gtttaaaact
    12961 ggtcaaaacc ataggcatag accgcaacaa ccgccgcgaa gccaacgaac agctcataaa
    13021 acaggggttg gcgcgcaaaa acgaaggcta ttggattacc attttccccg aaggcacgcg
    13081 ccttgcgccc ggaaaacgcg gcaaatacaa actcggcggc gcgcgcatgg cgaaaatgtt
    13141 tgagatggac atcgtccccg tcgccctcaa cagcggcgaa ttttggccga aaaactcctt
    13201 tctgaaatat ccgggggaaa tcaccgtcgt catctgtccg accatcccgc acgcaagcgg
    13261 cagcgaagcc gaattgatgg aaaaatgcga acacctcatc gaaacgcagc agccgctcat
    13321 ttccggcgca ggcccgtttg ccgccaaaat gccgtctgaa accgtatgac ctccctgatc
    13381 cacactcttt ctgacggcat ggaactgact atagaaatca agcgccgtgc caagaaaaac
    13441 ctgattatcc gccctatcgg cacacatacc gtccgcatca gcgtcccacc ctgcttctcc
    13501 gtctccgctc taaaccgctg gctgtatgaa aacgaagccg tcctgcggcg aacactggcg
    13561 aaaacaccgc cgcaaactgc cgaaaaccgg ctgcccgaat ccatcctctt ccacggcaga
    13621 caacttgccc tcaccgccca tcaagacacg caaatcctgc tgatgccgtc tgaaatccgc
    13681 gttcccgaag gcgcacccga aaaacagctt gcgctgctgc aggacttttt ggaacggcag
    13741 gcgcacagtt acctgattcc ccgcctcgaa cgccacgccc gcaccacaca actgttcccc
    13801 gcctcctcct cgctgacctc tgccaaaacc ttctggggcg tgtgccgcaa aaccacaggc
    13861 atacgcctca accggcggct ggtcggcgca ccggaatacg ttgccgatta tgtctgcata
    13921 cacgaactct gccacctcgc ccatcccgac cacagccccg ccttttggga actgacccgc
    13981 cgcttcgccc cctacacgcc cgaagcgaaa cagtggctca aaatccacgg cagggaactt
    14041 ttcgccttag gctgacgcgg tcccgggccg gcatccgcca attcagacct cctcccaaac
    14101 ggacatccgg aaagcagcat cacagccggc gacacatacc gctgccgccg ctttcagacg
    14161 gcatccgtgc cggaacaggc acgcgcccgc ccgattcaaa ccgcgatgac gctttgccgc
    14221 cggttcgggg caggatggcg gcacacgcgc cgtctgccgc gtttcatttc acaccgctct
    14281 tccgaaaccc gagacccgcc cggtccgacg tgcggtatga aacgcttaag ctggcgcgaa
    14341 gtcttttact gatttgcccg cgaaaatgcc gtctgaaagg ttttcggacg gcattttttt
    14401 gcgtttccca ggatggcggc ggattcgtaa aaggcggtca gggtggattg taggatgggt
    14461 tgagacctgc cgaatccgcc gcatctgcca aatctaccgc cgtcattcct acgaaagtgg
    14521 gaatctagaa cgcggggtta agaaaacctg catcccgtca ttcccacgaa agtggg
//
LOCUS       DAXHMX010000041        14132 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHMX010000041 DAXHMX010000000
VERSION     DAXHMX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.041"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..1707
                     /locus_tag="ACFM4S_000894"
     CDS             <1..1707
                     /locus_tag="ACFM4S_000894"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6050610.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSF
                     GYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFI
                     GNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGF
                     YKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQ
                     KLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRP
                     ELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKAVSRAFYQAEKKN
                     GE"
     gene            1697..4483
                     /locus_tag="ACFM4S_000895"
     CDS             1697..4483
                     /locus_tag="ACFM4S_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6050611.1"
                     /translation="MANNKTLFEVIENRKAVYLEDGDDEKYRLPKFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDSDNANEQGKTRTEQALKFMQE
                     KGVEFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIF
                     TVDRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAF
                     EGKQPNKRKFDNDMQHELRILEELFYYAHDEQSRYISELKNELRKDGYLPEKDDDKVL
                     ATFKLKSEFAANKDFRELLIWANKKIPNPNAKSNNADSLKANPQTLSFQVHGNQLLQE
                     TQFTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSK
                     LDIQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFI
                     GTKEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISA
                     RLGDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQI
                     FIEPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFT
                     ESFPLGAASLEKMK"
     gene            complement(4627..5799)
                     /locus_tag="ACFM4S_000896"
     CDS             complement(4627..5799)
                     /locus_tag="ACFM4S_000896"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6050612.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6149..6595
                     /gene="iscR"
                     /locus_tag="ACFM4S_000897"
     CDS             6149..6595
                     /gene="iscR"
                     /locus_tag="ACFM4S_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6050613.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6624..7838
                     /locus_tag="ACFM4S_000898"
     CDS             6624..7838
                     /locus_tag="ACFM4S_000898"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6050614.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLGHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7984..8829
                     /locus_tag="ACFM4S_000899"
     CDS             7984..8829
                     /locus_tag="ACFM4S_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFC6050615.1"
                     /translation="MKIANALIHNTVRIECLSADGQSISTGTAFLFLFDFDKTGVIPV
                     LVTNKHVVFVESAKKIAITLTKDENGSPNHKENITFTIEDFIQNCLPHPDENIDLCIA
                     PVGHFFNQLINHNFSPFIKGIRESDIMQTEEMDQLSAFEEVFMIGYPNGLWDSKNNLP
                     IFRTGITATHPAIDFEGKKEFMIDVACFPGSSGSPVFKYIPAGLRKIENDGFAITADE
                     TFKLMGILYAGPQQTISGEIYITTVNKPISVSSIPINLGFVIKAEKLLDFKPILKNFL
                     EKNRE"
     gene            8976..9104
                     /locus_tag="ACFM4S_000900"
     CDS             8976..9104
                     /locus_tag="ACFM4S_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050616.1"
                     /translation="MNEQELDLDNLDNLLDDFDGVTVEGGVDSENDEGCEGGACKI"
     gene            9172..9558
                     /gene="iscU"
                     /locus_tag="ACFM4S_000901"
     CDS             9172..9558
                     /gene="iscU"
                     /locus_tag="ACFM4S_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6050617.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            9645..9965
                     /gene="iscA"
                     /locus_tag="ACFM4S_000902"
     CDS             9645..9965
                     /gene="iscA"
                     /locus_tag="ACFM4S_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6050618.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(9925..10146)
                     /locus_tag="ACFM4S_000903"
     CDS             complement(9925..10146)
                     /locus_tag="ACFM4S_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC6050619.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            10228..10728
                     /gene="hscB"
                     /locus_tag="ACFM4S_000904"
     CDS             10228..10728
                     /gene="hscB"
                     /locus_tag="ACFM4S_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6050620.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            10826..13576
                     /gene="gyrA"
                     /locus_tag="ACFM4S_000905"
     CDS             10826..13576
                     /gene="gyrA"
                     /locus_tag="ACFM4S_000905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6050621.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIRLIKESPNAVEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            13733..14132
                     /locus_tag="ACFM4S_000906"
                     /pseudo
     CDS             13733..14132
                     /locus_tag="ACFM4S_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558014.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         4370 a         3389 c         3034 g         3339 t
ORIGIN      
        1 ttatacacta aattaacccg aaaaagacaa gaaatctttt ttaatcaaac gcttgctttt
       61 gatgaaatcg atcggctttt tgacgcaaaa gcattctcaa aattctctcg ctataccgca
      121 gacggcaaac aagcggttgg cgaaatcaaa cgtcattcag acggcacacc cgccgaaaat
      181 ctcattatca aaggcaataa tctgatagcc ctacattcgc ttgccaaaca gtttaaaggc
      241 aaagtgaagt tgatttatat tgatccgcct tattattttg ttaaaaagaa acaacaagat
      301 agttttggtt ataacactaa ctttaaatta tctacttggc ttacatttat gaaaaatcgt
      361 ttatcaattg ccaaagaatt attaactgat gatgggatta ttgttatttc cattgatgat
      421 gacggaaatg cttatttaaa gattttgtta gatgaaattt ttggatttga aaattttatc
      481 ggaaatttac ccactattat gaatttaaaa ggtaataatg atgaatatgc ttttgcaggc
      541 actcacgaat acacactagt ctttgctaaa aataaagata aatctacttt ttatgaattt
      601 ccaatagatg aagataattt tttagagaaa tgggaagaag atgaaattgg gttttataaa
      661 aaaggagctc caatgcgagc aacaggaacc gaagataaaa gagaagatag acctgaaatg
      721 ttctatcctt ttttggtaaa aaataacaca gttagtacaa ttacagatga agaattttct
      781 caaatctata ataaagattt agaagtattc aatgatgatt ttattcaaaa actaaaagag
      841 aaatacgaaa atcttggata caattttatt ttacctattg ctgataaaca atgggggcgt
      901 tggcgttggg ggtattcaat aaagaataag gcaaggctgc agacggatat tattgttagc
      961 caatctaaaa atggaatttc cttgtataaa aaacaaagac ctgaattaga tgatttacct
     1021 acaaaaaaac ctaaaactat tttttataaa cctgaataca gtagtggtaa tggaactgaa
     1081 cagatgaaaa atctttttgg tgaaaaggca tttaaaaatc ctaaaccaga agaacttatc
     1141 caagatttta ttaccatcac aaccaacgaa tccgacatcg tcttagacta ccatcttggt
     1201 agtggcacaa ccgccgccgt tgcccacaaa atgaaccgac aatatatcgg tattgaacaa
     1261 atggattata ttgaaacgct tgccgttgaa cgcttgaaaa aagtgattga tggcgagcaa
     1321 ggcggtattt ccaaagccgt gaattggcaa ggtggtggcg agtttgttta tgccgaactt
     1381 gccccattta acgaaaccgc aaaacaacaa attttggctt gcgaagattc agacggcatc
     1441 aaaacgctgt ttgaaggttt atgcgaacgc tatttcttga aatacaacgt cagcgtaaat
     1501 gaatttagtc aaatcattca agagcctgaa tttcaatctt tgccattaga cgaacaaaaa
     1561 caaatggtgc ttgaaatgtt agatttaaat caaatgtatg tttcattatc cgaaatggac
     1621 gacgagcaat ttgcagattg cctgaacgat gacgataaag cggtaagccg tgcgttctat
     1681 caagcggaga aaaaaaatgg cgaataataa aactttgttt gaagtaattg aaaaccgcaa
     1741 agctgtgtat ttggaagacg gagatgatga aaaatacagg ctgcctaaat ttgtttcaaa
     1801 taatttgaaa tatccgtttt ttgaatggca aaaatcggct ttagaaaact ttgtgatttt
     1861 tgaccgcact tcaaaattaa aggattttcc cgatatcaaa aaccgcccta ctcatttgct
     1921 atttaatatg gcaacaggtg caggcaaaac gatgatgatg gcggcattga ttttgtatta
     1981 ttttgaaaaa gggtatcggc attttctgtt ttttgtgaat caaaacaata tcgtggataa
     2041 aacggaaaat aattttattg acccgacaca cgcaaaattt ttatttaccg agaagatttt
     2101 gcagggcgat acggtaattc ccattcgcaa agtggaaacg ttcagcccac attcagacgg
     2161 cattgaaatt aaatttacca gcattcaaaa gctgtataac gatattcaca ccgaacggga
     2221 aaaccaaacc acattggcgg atttgcacga attgaatctt gtgatgctgg gcgatgaagc
     2281 acaccattta aacgcgcaaa ccaaaggcaa aaaacaaggc gagttggatt tggaagtgga
     2341 attaaaagcc aatgcaggca atgccgaaat tgaacgcaaa ggctgggagc atatggtttt
     2401 ggaattgtta ctcaataaaa atggcaatcc cagccaaaat gtgctgttgg aatttaccgc
     2461 cacgctgcct gaaaatgctg acgtgcaaca aaaatacgcg gataaaatca tcacaaaatt
     2521 tggcttaaaa gaatttttgc aaaaaggtta taccaaagaa atcaatttgg tatccagtac
     2581 gctgggcaag aaagagcggg tgttgcacgc tttattattt gcttggtatc gccatcaaat
     2641 cgctttgaaa tatggcattg ccaatttcaa gcctgtgatg ttgtttagaa gtaagacgat
     2701 tgatgaatca aaagcggatt atctggcatt tttaaattgg gcagaaaatg tgcaggcggt
     2761 tgatttttcg tttttaacta cattttcaac aagcttgaac gatagcgata gcgataacgc
     2821 caacgaacaa ggcaaaaccc gcactgaaca agccctaaaa tttatgcagg aaaaaggcgt
     2881 tgagtttgca catttggcag attgggtaaa acaaaattat caaaaacaca atgtgattat
     2941 taccaactcc gaaaccaaca aaaccaaaac cgaaaaaacc gacagcgaaa cagaaaaatt
     3001 gctgaataat ttggaagcgg ctgataatcc gattcgtgcc atttttacgg tggacagatt
     3061 aaccgaaggt tgggacgttc tgaatttgtt tgatattgtg cgtttgtatg aagggcaaaa
     3121 cggcggcggt tcaaataaaa aatcaggcaa aacggctgcc gccaccgtat cggaaaagca
     3181 gttgattggt cgtggcgtgc gttattttcc atttgcgttt gaaggtaaac agccgaataa
     3241 acgcaaattt gacaacgata tgcaacacga attgcgtatc ttggaagaat tgttttatta
     3301 cgcgcacgat gagcaatccc gctatatttc agaactgaaa aatgagttgc gaaaagacgg
     3361 ttatttgcct gaaaaagacg atgataaggt attggcaaca tttaaactca aatctgaatt
     3421 tgccgctaat aaggatttta gagaattgtt aatttgggcg aataaaaaaa tccccaaccc
     3481 aaacgccaaa agcaataatg cagacagcct aaaagccaat ccacaaacgc tttcattcca
     3541 agttcacggt aatcaactgt tgcaggaaac gcaatttaca gccgatgaaa atgatgaaac
     3601 agcccgacaa atcggcacac aaaataattt tactcaaacc ataaaaatga gtgaaatgga
     3661 gcggcacatt ttcaataaag cattacatat caaagggaaa aatagtcaat ctttattcca
     3721 tttcaaccgt ttgcaaagca agctcgacat tcaaaatcgc aatgaattgc aaaataactt
     3781 gttgaaagac tggcaaattg aatttttagg attagggcaa gacaaacagg ttcgcctgga
     3841 cgataaactt gcaggctgcc tgaaaatctt ggaaatggtt gaaaaacatc tgaatgaaag
     3901 tgatatgccg tttatcggta caaaagaatt tacacctaaa aaattatggg aaattttcgg
     3961 cacgccaaaa caaaaatggg tcaaaaaaga tgatgtaaaa acgactattg ccacgcaaaa
     4021 tgattggtat gtgatgaata attttgccgg aacgggtttg gaagaagcgt taattcaatt
     4081 tatttcagcg cgtttgggcg atttgaaatc taaatatgac gttcatttaa tccgtaatga
     4141 agaagtgttt aaattgaata actttgccga tggtgaagga tttatgccgg actttatttt
     4201 attgctgaaa gataaacaaa aatcttcttc caatggtgtg aacgactttt tgcattacca
     4261 aattttcatt gagcccaaag gtgagcattt agtggaaact gatggatgga aagaagaatt
     4321 tttagaagcc atcactgtgg aatatgggat ggataagatc ctgcaaaaag atacaccgca
     4381 ttatcgtttg atcggtttac cgttttttac tgatcatcag aaaaatggac aatttacgga
     4441 gtcattccct ttaggtgcgg catcgcttga aaaaatgaag tagtgcattg caggcaaccc
     4501 cgtttgacaa aacttccttt acaaaagggc gttttgtcag atatttaatc aacacattat
     4561 taaaatacag ccaaatttta atgccgtccg aaccctgtgt tcagacggca tcgtattttt
     4621 cagtatctaa accgtttccc tgccccaatc tttgcctctc aaaatcgaag catcgacatc
     4681 ttgaatatcg cggtgtcccg taaacgccat agatatatcc atttctttat acaggatttc
     4741 cagcgcacgg gttacgcctt cttcaccata cgcacccaaa ccatacagaa acgcccggcc
     4801 tatcatcgta cctttcgcgc ccaaagccca cgccttcaaa atatcctgac cgctgcggat
     4861 accgctgtcc atccaaactt cgatgtcgct gccgactgca ctaacgatgt cgggcaaggc
     4921 tttgatggcg gacacggtat cgtcgagctg gcggccgccg tggttggaaa caatcaatgc
     4981 gtccgcgccg cttttcgctg ctttttccgc gtcttcgggt tccataatgc ctttgataat
     5041 cagcttgccg ccccacaaat ctttaatacg cgccacatcg tcccagctca ggcgcggatc
     5101 gaattgttcg gaagtccatg aagacagcga ggacaaatcg ccgacgtttt tggcgtgtcc
     5161 aacgatattg cggaacgtgc ggcgttccgt gttcagcatt ttcatacacc attcgggctt
     5221 ggtcgccaga ttgattaaat tggcgatggt cggtttcggc ggcgcggaca ggccgttttt
     5281 gatgtctttg tggcgttgcc ccaaaacctg caaatcagcg gtcaatacca atgccgaaca
     5341 tttggcatcc ttcgcgcgct taatcaggtt ttccataaac tcgcggtcgc gcatcacata
     5401 aagctgaaac caaaatggtg cggaagtgtt ctcggcaacg tcttcaatcg agcagataga
     5461 catcgtggac aacgtaaacg gaatgccgaa cttctccgcc gcccgcgccg ccaagatttc
     5521 accgtctgcg tgcgccatac ccgtgaaacc agtcggggca atcgccaccg gcattttcac
     5581 atcctgccca atcattttgg tttcgaggct gcgcccctcc atattgacca atactttttg
     5641 tcggaaacga atgtctttga aatccgaagt attttcccga taagtcgttt ccgtccacga
     5701 acccgaatcg atgtaatcgt aaaacatacg cggcattttg cgcttggcaa cgcggcgcaa
     5761 gtcttcgatg caggtcattt tgctcaaatc acgtttcatc ttgtttttcc ctgtttgaca
     5821 gcctgatgga atatgccgtc cgaaaccgct tgagccttca gacagcattt caggcaatac
     5881 ccaaataaat cactaagatt tagataatct atatcaatat caaaaataaa tcaaatcgtt
     5941 ttgaaaccgc ttgccggcaa aataccccga ccgccgctcc tcaaaaaaca taaccaactg
     6001 atttaaaaaa ccaaacaaat aaagcaaaat cagactatac cgccatttaa acaaacaatc
     6061 ggcaacagct cccccttact tgactgaaac actcagatat tggacaattc tccccactat
     6121 aaaaaagccg acacgggcaa ccaccaccat gagactgacc accaaagggc gtttcgccgt
     6181 taccgctatg ctggatttgg cgatgaacgc gcaaaccggc gccgtcaaac tcagtgccat
     6241 cagcgaacgc caaaacatat ccctctccta tctcgagcaa ttgttcggca aactccgccg
     6301 cgccggactg gttgaaagcc tgcgcgggcc cggcggcggc tacatcctcg ccgcaccggc
     6361 ggcacgcatc aacatcgccc aaatcatcgc cgccgccgaa gaccggctgg acgcgaccca
     6421 atgcggcagc aaagccaact gccaccacgg tgcgccctgc ctgacgcacg acctttggga
     6481 gaatttaaac aaaaccatca acgactacct cggcagcgtt accctgcaaa gcatcatcga
     6541 acagaaaaac aacggcgacg gcagccgcgt cgtccaattt acacacatcc attaaataac
     6601 acccgaaaaa gaaagagcaa accatgaccg tcaaaacccc cgtttacctc gactacgccg
     6661 ccaccaccac cgttgacaaa cgcgttgccg aaaaaatgat tccctatctg accgaaacct
     6721 tcggcaaccc cgcctccaac agccacgcat tcggctggga agcagaagag gccgtcgaaa
     6781 aagcccgcgc cgacatcgct gccctgatta atgccgaccc caaagaaatc gtcttcacca
     6841 gcggcgcgac cgagtccgac aacctcgcca tcaaaggcgc ggcaaacttc tacaaaacca
     6901 aaggcaaaca cctcattacc gtcaaaaccg aacataaagc cgttttagat acaatgcgcg
     6961 aactcgaacg ccaaggtttt gaagtaacct acctcggcgt tcaagaaaac ggtttgattg
     7021 atttggaaga actcaaagcc gccatccgcg acgacaccat cctcgtttcc gtaatgtggg
     7081 caaacaacga aatcggcgtg gtgcaagaca ttcccgccat cggcgaaatc tgccgcgaac
     7141 gcaaaatcgt cttccacgtc gatgccgccc aagcctgcgg caaagtgcct gtcgatgtcg
     7201 aagccgccaa aatcgacctg ctctcgatgt ccgcgcacaa agtgtacggc cccaaaggca
     7261 tcggcgcgct gtacgtccgc cgcaaacccc gcgtccgcct cgaagcccaa atgcacggcg
     7321 gcggccacga acgcggcttc cgcagcggca cattgccgac ccaccaaatc gtcggtatgg
     7381 gcgaagcctt ccgcattgcc aaagaagaat tggcacaaga cactgcgcac tacctgaaac
     7441 tgcgcgatat cttcctcaaa ggcatcgaag gcatcgaaga agtctacatc aacggcgacc
     7501 tcggacaccg cgccccgaac aacctgaacg tcagcttcaa cttcgtcgaa ggcgaaagcc
     7561 tgattatggc ggtgaaagaa ctcgccgtat ccagcggctc cgcctgcacc tccgcctcgc
     7621 tcgaacccag ctacgtcctg cgcgcgctcg gccgcaacga cgaactggcg cactcatccc
     7681 tgcgtatcac cttcggtcgc atgaccaccg aagaagaagt gcaattcgcc gccgaactga
     7741 ttaaatccaa aatcggcaaa ctgcgcgaac tgtcgccgct gtgggaaatg ttcaaagacg
     7801 ggattgattt gaactcgatt gaatgggcgg cgcattaatt tatgaattag tacgatgata
     7861 tgagtatagt agataagatt ttcaaatatt ctcgccacct cgattattga gatatagtga
     7921 acttcttatc tacggataaa atttttatag atagtttaat atttaaatag aacttaaaaa
     7981 attatgaaaa tagccaatgc attaattcac aatacagtcc gtattgaatg cctttcagca
     8041 gacggccaat ctatatcaac gggaacggca ttcttattcc ttttcgattt cgacaaaaca
     8101 ggggtaattc cagttcttgt aacaaataaa catgttgttt ttgtagagtc cgcaaaaaaa
     8161 atagctatta ctttgacaaa agatgaaaat ggctctccaa atcataaaga aaatataact
     8221 tttacaattg aagattttat acaaaattgt ttacctcatc ctgatgaaaa tattgatcta
     8281 tgcattgctc ctgttggaca tttttttaat cagctgatta atcataattt ctcgcctttt
     8341 ataaaaggaa taagagaatc tgatattatg caaactgaag aaatggatca attatcagct
     8401 ttcgaagaag tttttatgat aggctatcct aatggcttat gggactcaaa aaacaactta
     8461 ccaatattta gaacaggaat tactgctacc catccagcta ttgattttga agggaaaaaa
     8521 gaatttatga ttgatgttgc ctgtttccct ggttcaagcg gttctccggt gttcaaatat
     8581 atacctgctg ggttaagaaa aatagaaaac gatgggtttg caattacagc agatgaaaca
     8641 tttaaattaa tgggaatcct atatgctgga cctcagcaaa caatatcagg ggaaatttat
     8701 ataacaactg ttaataaacc aatttctgta tcttctattc ctattaattt aggttttgtc
     8761 ataaaggcag aaaaactttt agactttaaa ccaattttaa agaatttttt ggaaaaaaat
     8821 agagaatgaa attatattaa aagattaata aatattagag actggcaatc caccttacct
     8881 aatctacatt aaaaataaaa aagaccgttt gaaacatcaa aaagattttt tcaaacgacc
     8941 taaaagccac cacatgaaat caaggataca aaaccatgaa cgaacaagaa cttgacctcg
     9001 acaatctcga caacctgctt gacgattttg acggcgttac cgtcgaaggc ggcgtcgatt
     9061 cggaaaacga cgaaggctgc gaaggcggcg cgtgcaaaat ctgactcagg ccgtctgaaa
     9121 accggcaaac aaaccacttt aaactatcaa aaacattaag gaaaccacat catggcatac
     9181 agcgataaag taatcgacca ctacgaaaat ccccgcaacg tcggtacttt cgacaaaaac
     9241 gacgaatctg tcggcaccgg catggtcggc gcgcccgcct gcggcgacgt catgcgcctg
     9301 caaatcaaag tgaacgacga gggcatcatc gaagatgcga aatttaaaac ttacggctgc
     9361 ggctcggcca tcgcttcgtc cagcctgatt accgagtggg ttaaaggcaa aagcctggat
     9421 gacgcgctgg caatcaaaaa cagcgaaatc gccgaggagt tggaattgcc gccggtaaaa
     9481 atccactgct ccatcttggc tgaagatgcg gtaaaagcgg ccgttgccga ctaccgcaaa
     9541 cgtcaggaaa acagataaag cccttcagac ggcatcatcc cgcaatgccg tccgaaccac
     9601 ccgccgcttc aggtccgtcc ggggcggtac aacaaggaag aaatatgatt acccttaccg
     9661 agaatgccgc aaaacacatc aatgactatc tcgccaaacg cggcaaaggc ttgggcgtac
     9721 gcttgggtgt gaaaaccagc ggctgctcgg ggatggcgta caaccttgaa tttgtcgacg
     9781 aagccgatgg cgacgacctg attttcgaag gacacggcgc gcgcatttat atcgatccga
     9841 aaagcctggt ttatctggat ggcacgcaag tcgattacac caaagaaggt ttgcaggaag
     9901 gtttcaaatt tgaaaacccc aatgtcaaag actcctgcgg ctgcggcgaa agcttccacg
     9961 tttaaggcat aaaaacggcg ggaccgtatc aaaaccgtcc cgccattttt tcgcttcctg
    10021 cctgttgtag ctgcctttgc ctttcctttt ccgttccacc ttgtgccgga acaaatcgga
    10081 tttcactaag gcttttaaag cattgtcgcg tattttgcct ttattgtgct gcactttgcc
    10141 gcccatattc agtcctttcg tttaagaagc ggcagattat aaggcaaaaa cagttttctg
    10201 ccaaaatctt acatttatca tcctactatg tcccaatatt tcaccctctt ccggattgaa
    10261 cccgctttcg atatcgacac cgaaaacttg gaacaaacct accgcgcctt ggccgcccgt
    10321 ttccatcccg ataaattcgc ttcagcttcc gcctttgagc aaaagcaggc agtgatgatg
    10381 tcttccacca tcaacgatgc ctaccgcacc ttgaaaaacc ccatcgaccg cgccgcctac
    10441 ctgctgaaaa catcgggcat cgatgccgac gcgccggagc ataccgcttt cgcccccgaa
    10501 ttccttatgc agcaaatgga atggcgcgaa acgctgatgg aggcacgggc aggcaacgac
    10561 cttgaatcct tgaaaaatct cgacaacgaa atccgcgacg agcaagaaaa actgttctgc
    10621 ggtctgaaac agtcgtttgc ccgacaagat tacgacacag ccgcacaaca agtccgccaa
    10681 ggcaggtttc tcgacaaact ccgcaacgaa atttcctcgg cattataatc cgcaccgtgt
    10741 ttcagacggc gtaaccgccg caccgttccg cgtcaaaata tgctaaaata agcaacaatt
    10801 ttttgccata cgaaacattg aaaccatgac cgacgcaacc atccgccacg accacaaatt
    10861 cgccctcgaa accctgccgg taagccttga agacgaaatg cgcaaaagct atctcgacta
    10921 cgccatgagc gtcattgtcg ggcgcgcgct gccggacgtt cgcgacggtc tcaagccggt
    10981 acaccgccgc gtactgtacg cgatgcacga attgaaaaac aactggaatg ccgcctacaa
    11041 aaaatcggcg cgcattgtcg gcgacgtcat cggtaaatac cacccccacg gcgataccgc
    11101 cgtatacgac accatcgtcc gtatggcgca aaatttcgct atgcgttatg tgctgataga
    11161 cggacagggc aacttcggat cggtggacgg gcttgccgcc gcagccatgc gctacaccga
    11221 aatccgcatg gcgaaaattt cccacgaaat gctggcagac attgaggaag aaaccgtcaa
    11281 tttcggcccg aactacgacg gtagcgaaca cgagccgctt gtactgccga cccgtttccc
    11341 cacactgctc gtcaacggct cgtccggcat cgccgtcggc atggcgacca atatcccgcc
    11401 gcacaacctt tccgataccg tcaatgcctg cctgcgcctg ctcgatgcac ccgacaccga
    11461 aatcgacgaa ctgatcgaca ttatccaagc ccccgacttc ccgaccgggg caaccatcta
    11521 cggcttgagc ggcgtgcgcg aaggctataa aacaggccgc ggccgcgtcg ttatgcgcgg
    11581 taagacccat atcgaaccca taggcagaaa cggcgaacgc gaagccatcg ttatcgacga
    11641 aatcccctat caggtcaaca aagccaagct ggtcgagaaa atcggcgatt tggttcggga
    11701 aaaaacactg gaaggcattt ccgagctccg cgacgaatcc gacaaatccg gtatgcgcgt
    11761 cgttatcgag ctgaaacgca acgaaaatgc cgaagtcgtc ttaaaccaac tctacaaact
    11821 gactccgctg caagacagtt tcggcatcaa tatggtggtt ttggtcgacg gacaaccgcg
    11881 cctgttgaac ctgaaacaga ttctctccga attcctgcgc caccgccgcg aagtcgttac
    11941 ccgacgtacg cttttccggc tgaagaaggc acgccatgaa gggcatatcg ccgaaggcaa
    12001 agcggtcgca ctgtccaata tcgatgaaat catccggctt atcaaagaat cgcccaacgc
    12061 ggtcgaggcc aaagacaaac tgcttgcgca cccttggcgc agtagcctcg ttgaagaaat
    12121 gctgacgcgt tccggtctgg atttggaaat gatgcgtccg gaaggattgg ctgcaaacat
    12181 cggcttgaaa gagcaaggtt attacctgag cgagattcag gcagatgcta ttttacgcat
    12241 gagcctgcga aacctgaccg gcctcgatca agaagaaatt gtcgaaagct acaaaaacct
    12301 gatgggtaaa atcatcgact ttgtggatat cctctccaaa cccgaacgca ttacccaaat
    12361 catccgcgac gaactggaag aaatcaaaac caactatggc gacgaacgcc gcagcgaaat
    12421 caacccgttc ggtggcgaca ttgccgatga agacctgatt ccgcaacgcg aaatggtcgt
    12481 taccctgaca catggcggct atatcaaaac ccagccgacc accgactatc aggcgcagcg
    12541 tcgcggcggg cgcggcaaac aggcggctgc caccaaagac gaagacttta tcgaaaccct
    12601 gtttgttgcc aacacgcatg attatttgat gtgctttacc aatttgggca agtgtcattg
    12661 gattaaggtt tacaaactgc ccgaaggcgg acgcaacagc cgcggccgtc cgattaacaa
    12721 cgtcatccag ttggaagaag gcgaaaaagt cagcgcgatt ctggcagtac gcgagttccc
    12781 cgaagaccaa tacgtcttct tcgccaccgc gcagggaatg gtgaaaaaag tccaactttc
    12841 cgcctttaaa aacgtccgcg cccaaggcat taaagccatc gcgctcaaag aaggcgacta
    12901 cctcgtcggc gctgcgcaaa caggcggtgc ggacgacatc atgctgttct ccaacttagg
    12961 taaagccatc cgcttcaacg aatactggga aaaatccggc aacgacgaag cggaagatgc
    13021 cgacatcgaa accgaaattt cagacggcat cgaagatgaa accgccgaca gcgaaaacgc
    13081 actgccgagc ggcaaacacg gtgttcgccc gtccggtcgc ggcagcggcg gtttgcgcgg
    13141 tatgcgcctg cctgccgacg gcaaaatcgt cagcctgatt accttcgccc ctgaaaccga
    13201 agaaagcggt ttgcaagttt taaccgccac cgccaacgga tacggaaaac gcaccccgat
    13261 tgccgattac agccgcaaaa acaaaggcgg gcaaggcaat attgccatta acaccggcga
    13321 gcgaaacggc gatttggtcg ccgcaacctt ggtcggcgaa accgacgatt tgatgctgat
    13381 taccagcggc ggcgtactta tccgcaccaa agtcgaacaa atccgcgaaa ccggccgcgc
    13441 cgcagcaggc gtgaaactga ttaacttgga cgaaggcgaa accttggtat cgctggaacg
    13501 tgttgccgaa gacgaatccg aactctccga cgcttctgta atttccaatg taaccgaacc
    13561 ggaagtcgag aactgaaaat catctcccga tgccgtctga agattcagac ggcatttatt
    13621 ttatccctca tccgtcatcc ggcttctcac aatatagcgg attatagtca attaaaaaca
    13681 aggggctgtc ctagataact agggaaattc aaattaagtt agagttgccc ctatgagaaa
    13741 aagtcgtcta agccggtata aacaaataaa ctcattgaac tgtttgtcgc aggtgtaact
    13801 gcaagaacga cagcagagtt agtaggcgtt aataaaagta ccgcagccta ttattttcat
    13861 cgtttacgat tacttattta tcaaaacagt ccgcatttgg aaatgtttga tggcgaagta
    13921 gaagcagatg aaagttattt tgctgaacga caaaaccata ttaatggaat tgagaacttt
    13981 tggaaccggg caaaacatca tttacgcaag tttgacggca ttcccaaagc gcattttgag
    14041 ctgtatttaa aggggtacga acggcgtttt aacaacagtg agataaaagt tcaaatttcc
    14101 attttaaaac aattagtaaa atcgagttta tc
//
LOCUS       DAXHMX010000042        13699 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHMX010000042 DAXHMX010000000
VERSION     DAXHMX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.042"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            325..5808
                     /gene="iga"
                     /locus_tag="ACFM4S_000907"
     CDS             325..5808
                     /gene="iga"
                     /locus_tag="ACFM4S_000907"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6050622.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWGASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRVGAGRQLVYEKGAYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNTHYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQRDNAGTIKG
                     NREHHWNITFGTNSKIGSTAVRLANNERDANNGQNVTFEDNGTLVLDQNINQGAGGLF
                     FKGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQ
                     GQLKVGDGTVILNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGR
                     LDANGNDLTFEHIRNVDEGARIVNHNTDRASTITLTGKSLITAPQNLSVYEIRNDYDD
                     DDYYGYYSYRKPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWVFMGYKQE
                     EAQKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGK
                     ISVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVN
                     QSASFSSGRNVSNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKAL
                     NSFGATQINGNVNLSQNAALVLGKAALWGQIQGQGNSSVSLNQHSKWHLTGDSQVHNL
                     SLADSHIHLNNASDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGH
                     YQLHVQDKTGEPNQEGLNLFDASSVQDRSHLSVSLANNHVDLGALRYTIKTENGITRL
                     YNPYAENRRRVKPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAK
                     RQQAKAEQVKRQQAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEK
                     EREAAELSAKQKVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQR
                     VGEEERRQTAQSQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELVR
                     RQQEERKAAELLAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELS
                     ANQKAAAEAQALAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAA
                     KRQQEARQTAELARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQP
                     QRRKRRAAPQDYMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDY
                     EEIPLDALEDENVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTS
                     DKQPQDNTELHEKVETVSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMA
                     STQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQI
                     GIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAG
                     SLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDS
                     VKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFAYKADN
                     QQKYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(5986..7188)
                     /gene="trpB"
                     /locus_tag="ACFM4S_000908"
     CDS             complement(5986..7188)
                     /gene="trpB"
                     /locus_tag="ACFM4S_000908"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6050623.1"
                     /translation="MKNYHAPDEKGVFGEHGGLYVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(7373..8062)
                     /locus_tag="ACFM4S_000909"
     CDS             complement(7373..8062)
                     /locus_tag="ACFM4S_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6050624.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLRERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            8259..9038
                     /gene="rsmA"
                     /locus_tag="ACFM4S_000910"
     CDS             8259..9038
                     /gene="rsmA"
                     /locus_tag="ACFM4S_000910"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6050625.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGRADDFEYFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVDINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            9211..9939
                     /locus_tag="ACFM4S_000911"
     CDS             9211..9939
                     /locus_tag="ACFM4S_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6050626.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            10012..10371
                     /locus_tag="ACFM4S_000912"
     CDS             10012..10371
                     /locus_tag="ACFM4S_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050627.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            10376..10816
                     /locus_tag="ACFM4S_000913"
     CDS             10376..10816
                     /locus_tag="ACFM4S_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6050628.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKESYRRLKVWILWRQPKKTAETDTPS"
     gene            10848..12122
                     /gene="folC"
                     /locus_tag="ACFM4S_000914"
     CDS             10848..12122
                     /gene="folC"
                     /locus_tag="ACFM4S_000914"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6050629.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYHFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDMPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            12135..13133
                     /gene="ftsN"
                     /locus_tag="ACFM4S_000915"
     CDS             12135..13133
                     /gene="ftsN"
                     /locus_tag="ACFM4S_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6050630.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINERLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAFEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            13126..13623
                     /locus_tag="ACFM4S_000916"
     CDS             13126..13623
                     /locus_tag="ACFM4S_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6050631.1"
                     /translation="MNSLPIADLLVSAVIAACIVISTMRGVITEAGSMAAWVVSFFFA
                     KLFAAPFADIAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         3984 a         3597 c         3305 g         2813 t
ORIGIN      
        1 cagggttaag aaaacctaca tcccgtcatt ccctcaaaaa cagaaaacca aaatcagaaa
       61 cctaaaatcc cgtcattccc gcacaagcgg gaatccaaaa acaaaaagcc gcagaaattt
      121 aacggaagaa ccgaaccccg aaaatcggct tcccaccttc gcctgaataa cagaacaaag
      181 gaagcggaaa taaaaacatc gggattcttt ttttaaattg gcaataaaac accaaaatga
      241 atgatttaca caacaaaaaa tagtcaacac caccaaaccg gcgtaaaata caaaacatta
      301 tcacccttaa aacggtaaaa ccttatgaaa accaaacgtt ttaaaattaa cgccatatcc
      361 ttatccatct ttcttgccta tgcccttacg ccatactcag aagcggcatt ggtcagagac
      421 gatgtcgatt atcaaatatt ccgtgacttt gcagaaaaca aaggcaaatt ttttgtcggc
      481 gcaaccgatt tatcagtgaa aaacaaacaa ggtcaaaaca tcggcaacgc attatccaac
      541 gtaccgatga ttgattttag cgttgcagat gtcaacaaac gcatagcaac ggtagtcgat
      601 ccccaatatg ctgtcagcgt caaacacgcc aaagcagaag tccatacttt ttattacggt
      661 caatacaacg gtcataatga tgtagccgac aaagaaaatg aataccgcgt tgtcgaacaa
      721 aataactatg aaccccataa agcttggggt gcgagcaact taggtcgcct cgaagattat
      781 aatatggcgc gttttaacaa gtttgtaact gaagttgcgc cgattgcacc aactgatgcc
      841 ggcggcggat tggataccta caaagataaa aaccgttttt cctcttttgt cagagttggc
      901 gcaggcaggc aattagttta tgaaaaaggg gcttatcatc aagaaggaaa tgaaaaaggc
      961 tacgatttgc gcgatctttc acaagcctat cgttatgcca ttgcaggtac accttataaa
     1021 gatattaata ttgaccaaac aatgaatacc gaaggtttga ttggctttgg taatcacaat
     1081 acacactatt cagcagaaga acttaaacaa gcactctcgc aagatgcgtt aacaaattac
     1141 ggcgtgttgg gcgatagcgg ctctccgtta tttgcttttg acaaacaaaa aaatcaatgg
     1201 gtctttttgg gaacttatga ttattgggca ggttacggaa aaaaatcttg gcaagaatgg
     1261 aatatctata aaaaagaatt tgcagataaa atcaaacaac gcgataacgc cggcaccatc
     1321 aaaggtaata gagaacatca ttggaacatt acttttggca caaacagcaa gattggttct
     1381 acagcggtaa ggcttgccaa caatgaaagg gatgcaaaca acggacagaa tgttaccttt
     1441 gaagacaacg gcactttggt attggatcaa aacatcaacc aaggcgcggg cggtctgttt
     1501 ttcaaaggcg attacacagt caaaggtgca aataatgaca tcacttggct aggtgcgggg
     1561 attgatgttg ccgacggcaa aaaagtcgtt tggcaagtca aaaatccgaa tggcgacaga
     1621 ttggcaaaaa tcggcaaagg cacattagaa ataaacggca caggcgttaa ccaagggcaa
     1681 ttaaaagtcg gcgacggtac ggttattctg aatcaacaag ccgatgccga caaaaaagtc
     1741 caggctttct cccaagtcgg cattgtcagc ggacgcggta cattggtatt aaatagttca
     1801 aatcagatta atcccgataa cttatatttc ggtttccgtg gcggtcgttt ggatgccaat
     1861 ggcaatgact tgacttttga acacatccgc aacgtggatg aaggcgcgcg cattgtcaac
     1921 cacaacacag accgcgcctc cacaatcaca ttaaccggca aatcattaat taccgctcct
     1981 caaaatcttt ctgtttatga aatccgaaat gattacgatg atgacgatta ttatggctat
     2041 tacagctata gaaaacccat tccgcaaggc aaagatcttt attacaaaaa ctatcgttat
     2101 tacgccctaa aatccggcgg cagcgtgaac gcaccgatgc ccgaaaacgg acaaacggaa
     2161 aataacgact gggtctttat gggatataag caagaagaag cccagaaaaa cgcgatgaac
     2221 cacaaaaaca atcagcgtat tagcggtttt agcggtttct ttggcgaaga aaacggaaaa
     2281 ggacataacg gcgcattaaa ccttaatttc aacggcaaaa gcgcgcaaaa ccgtttcttg
     2341 ttaacaggcg gcacgaattt aaacggaaaa ataagcgtaa ctcaaggcaa tgtcttgtta
     2401 tcaggtcgtc caacaccaca cgcaagagat tttgtgaaca aatcttcagc ccgaaaagac
     2461 gcacatttct ccaaaaacaa tgaagtcgta tttgaagacg actggataaa ccgcacattc
     2521 aaagccacag aaattgcggt taatcaatcc gcatcattct cttccggcag aaatgtatcc
     2581 aacattaccg ccaacatcac cgcaacagac aacgccaaag taaatttggg ttacaaaaac
     2641 ggcgatgaag tttgcgtgcg ctcggactat accggttacg ttacctgcaa cacagacaac
     2701 ttatccgata aggctttaaa cagctttgga gcgacacaga ttaacggcaa tgtgaatttg
     2761 agtcagaatg cggcattggt cttaggcaag gctgcattat gggggcaaat tcaagggcaa
     2821 ggaaacagca gcgtcagcct aaaccaacat agcaaatggc atttgaccgg cgacagccaa
     2881 gtacacaatc tgtcattggc ggatagccat attcatttga acaacgcttc cgacgcgcaa
     2941 agtgcaaata aataccacac gctcaaaatc aatcatttat ccggtaacgg gcattttcat
     3001 tatctgacgc acctggcgaa aaatcttggg gataaggtgc ttgtgaagga atccgcatcc
     3061 ggtcattatc agctccatgt tcaagataaa acaggcgaac ccaatcagga agggctgaat
     3121 ctctttgatg catcatccgt acaagaccgt tcgcaccttt ctgtttcctt ggcgaataac
     3181 catgtagatt taggcgcatt gcgttataca atcaaaacag aaaacggtat tacccgattg
     3241 tacaatcctt atgccgagaa ccgccgccga gtcaaaccgg ccccgtctcc tgccacaaac
     3301 acggcttctc aagcacaaaa ggcaacacaa acggacggtg cacaaattgc caagcctcaa
     3361 aatatcgtcg tcgcaccacc tagcccgcag gcaaatcaag ccgaagaagc aaaacgccaa
     3421 caagcaaaag cggagcaagt gaagcgtcag caagcagaag cagagcgtaa atcggcagag
     3481 ttagcaaaac aaaaagcaga agccgaacgc gaagcaagag agctggcgac ccgccaaaaa
     3541 gcagaacaag aacgcagcag cgccgaactt gcacgtcgac atgaaaaaga acgcgaagcg
     3601 gcagagttgt cggcaaaaca aaaagtcgaa gccgaacgtg aggctcaagc attggcggta
     3661 cgccgaaaag ccgaagccga agaggcaaaa cgccaagctg cagagcttgc acgccggcat
     3721 gaaaaagaac gcgaagcggc agagttgtcg gcaaaacaaa gggtcgggga agaggaacgc
     3781 cgtcaaacag cccaatccca gccgcaacgc cgcaaacgcc gtgccgcacc gcaggattat
     3841 atggcagctt cccaagaccg tcctaaacgc cgcggacacc gttcggtaca gcaaaacaat
     3901 gtggaaattg cccaagccca agcagaactt gtgcgccgac agcaggaaga acgcaaagcg
     3961 gcagagttgt tggcaaaaca aagggcagaa gccgaacgtg aggctcaagc attggctgcg
     4021 cgccgaaaag cagaagccga agaggcaaaa cgccaagctg ccgaacttgc acatcgccag
     4081 gaagcagaac gcaaagcggc agaattgtcg gcaaaccaaa aagccgcagc tgaagcccaa
     4141 gcattggcgg cacgccaaca aaaagccctt gcgcgtcaac aggaagaagc acgaaaagct
     4201 gcagagttag ccgtcaaaca aaaagcggaa accgaacgca aaaccgccga acttgccaag
     4261 caaagggcag cagccgaagc agcaaaacgc cagcaagaag ctcgccagac cgccgaactt
     4321 gcacgtcgtc aagaagcgga acgccaagca gcagagttgt cggcaaaaca aaaagccgaa
     4381 acagatcgag aagcggcaga atcggcgaaa cgaaaagctg aggaagaaga gcaccgtcaa
     4441 gcagcccaat cccagccgca acgccgaaaa cgccgtgccg cacctcagga ttatatggca
     4501 gcttcccaaa atcgtccgaa acgtcgcgga cgcagatcta ctctgccggc accgccctcg
     4561 ccatcatttg attcgtcagc ttacgcagca cccagggcct tgcataatcc ggactggtat
     4621 gaaaatgact atgaagaaat ccccttggat gcgttggaag atgaaaatgt atctgaatca
     4681 gttgacacat cagacaaaca gcctcaagac aatacggaac ttcatgaaaa atatgaaaat
     4741 gattatgaag aaatcccctt ggatgcgttg gaagatgaag atgtatccga atcggttgac
     4801 acatcagaca aacagcctca agacaatacg gaacttcatg aaaaagttga gacagtgagt
     4861 ttgcaaccaa gagccgcgca gccgcgagcc caagccgcca ctcagccgca agcccaagcc
     4921 gccgcgcaag ccgatgcagt cagcaccaat accaactcgg ctttatctga cgcaatggca
     4981 agcacgcaat ctatcttgtt ggatacaggt gcttcattaa cacggcacat tgcacaaaaa
     5041 tcacgcgctg atgccgaaaa aaacagcgtt tggatgtcga acatcggtta tggccgtgat
     5101 tatgcttccg cacaatatcg ccggtttagt tcgaaacgca cgcaaacaca aatcggcatt
     5161 gaccgcagct tgtccgaaaa tatgcagata ggcggcgtat tgacttactc tgacagtcag
     5221 catacttttg atcaggcgag cggcaaaaat acttttgtgc aagccaacct ttatggtaag
     5281 tattatttaa atgatgcttg gtatgtggcc ggcgatattg gtgcaggcag cttgagaagc
     5341 cggttacaaa cacagcaaaa agcaaacttt aaccgaacaa gtatccaaac cggccttact
     5401 ttgggcaata cgctgaaaat caatcaattc gagattgtcc ccagtgcggg aatccgttac
     5461 agccgcctgt catctgcaga ttacaagttg ggtaacgaca gtgttaaagt aagttctatg
     5521 tcagtgaaaa cactgacggc cggactggat tttgcttatc ggtttaaagt cggcaacctt
     5581 accgtaaaac ccttgttatc tgcagcttac tttgccaatt atggcaaagg cggcgtgaat
     5641 gtgggcggta attccttcgc ctataaagca gataatcaac agaaatattc agcaggtgcc
     5701 gcgttactgt accgtaatgt tacattaaac gtaaatggca gtattacaaa aggcaaacaa
     5761 ttggaaaaac aaaaatccgg acaaattaaa atacagattc gtttctaaaa tactaaattc
     5821 atagcaaaat aaaatgccgt ctgaactcag gcttcagacg gcatttttat agtggattaa
     5881 caaaaatcag gacaaggcga cgaagccgca gacagtacag atagacggca aggcgaggca
     5941 acgccgtact ggtttttgtt aatccactat atcagacgga cgaggttaca gtttaatccc
     6001 tttgagcttc gcgacggtat tgatgtcttt gtcgccacga cctgagaggt tgaccagaat
     6061 cacttggtct ttacccattt tcggcgcatt tttcaccgcc caagcaacgg cgtggctgga
     6121 ttcgagcgcg gggatgatgc cctcgaagcg gcagagcaag tcaaaggctt cgagtgcttc
     6181 gtcgtctttg gcaacggtgt attcgacgcg cttgatgtcg tgcagatggc tgtgttccgg
     6241 gccgatgccg gggtaatcca agcctgcgga aacagagtgt gtacccaaga cttgaccgtt
     6301 ttcgtcctgc atcagatagc tgcggaaacc gtgcaatacg ccaatcggtg agcccgaagt
     6361 aatcggcgcg gcgtgatcgg gggtgttcac gcccaaaccg ccagcctcca cgccgacgag
     6421 gcgcacgttt tcttcgccga tatacgggtg gaacaaacca atggcgttcg atccgccgcc
     6481 cacgcaggca acggcaacgt cgggctgtct gccgatggct tccaacattt gttctttggc
     6541 ttcattgccg atcacgcatt gaaagtcgcg caccatttcg ggatacggcg cgggaccggc
     6601 ggcggtaccg atgatgtaga atgtgtcatc cacgcgggcg acccattcgc gcatggcttc
     6661 gttcatcgcg tctttcaaag tacggctgcc gctgtcaacg ccgacgacat tcgcgcccaa
     6721 taatttcata cggaacacgt tgggcatttg gcgttggatg tcgtccgcac ccatgtacac
     6781 gtcgcaagtc atgccgaagc gggcggcaac agtggcactc gccacgccgt gctgacccgc
     6841 gccggtttcg gcgatgacgc gttttttacc catacgtttt gccaacagtg cctgaccgat
     6901 ggtgttgttg actttgtgcg cgccggtgtg gttcaagtct tcgcgcttca accaaatttg
     6961 cgcgccaccc aaatgctcgg acaatcgcgc ggcatggtaa acggggctgg gcctgccgac
     7021 gtagtgtttc aaatcgcggt ggaattcttc ccaaaacgac gggtcgtttt tcgctgcctt
     7081 ataggcatcc gccagctctt gcaaggcggg aatcagggtc tcggaaacat aaagcccacc
     7141 gtgttcgccg aaaacgccct tctcgtcggg tgcgtggtaa ttcttcatca gatttcgtcc
     7201 ttgtgcttca aattttcaga cggcaattta tagtggatta acaaaaaccg gtacggcgtt
     7261 gccccgcctt gccgtactat ttgtactgtc tgcggcttcg ccgccttgtc ctgatttttg
     7321 ttaatccact atatttttca aacatgccgt ccgaacggga aattgtcggc aattataact
     7381 gcttttcaca caagcccgcc atttcaaaaa acgcccgata cgccgccgct tttttcgcca
     7441 aactcaaagg ataggcacgc gaagtcgaag gcaggcgcgt cagcgtcaaa tcccgtccgg
     7501 caaacggaaa cggcacggtt tcgcccgttt tcggcatttt gatgccgcca ccctgaatat
     7561 cgagcaggat ttccgtcgcc ttgccgcctg tcgtacaaat atggcggcac tcgggtattt
     7621 ttgccaacac cgccgccaaa tcgacggttt caactacctt taaaaacttg tcggacgcat
     7681 tgccgtgttc ccttgccgcc ttcaacacgg tcggacagga cgcaattccc cgttcgcgca
     7741 aaaacgcctt gattttctcg gcatcaaacg ctttttcaga caacctttgg aaatgcgccg
     7801 catcattaaa aaacaccagc ccgtaaacgc gccacatatc gttttggaaa ttcggataat
     7861 gaaactgcat cgcgcgtttg tcttccttgg gcggaaacgt ccccatcatc atgaccgcag
     7921 ccttcggcgg caacacggaa tcgaagggat gggtttcgat ttcaagcaga ttttcagaca
     7981 cgttcaatcc tccaacaggc aaaacggcag acagccggtt tttgcaccaa accgccaaac
     8041 acggcaagcc ttcaaacagc attatcacgc cctgccgttt tcggttacaa tgccgacatc
     8101 aaacggatac tgtaaacaca tttatgttcc aacacacagg acgacactta aagcaccgcc
     8161 ctatgtgcca tcctgatttg gaaggggtta cgcccctccc aaatagagtc tgattctacc
     8221 gccctaaagg gcggggtttc aaccgaaaag gaaacacgat gaaagaacac aaagcccgca
     8281 agcgtttcgg gcagaatttt ttgcaggaca cgcggattat cagcgatatt gtcaacgccg
     8341 tgcgcccgca ggcggatgat gtcgtgattg aaatcggtcc gggtttggcg gcgattaccg
     8401 aacctttggc gaaaaagctg aaccgcctgc acgttgtcga aatcgaccgc gacatcgtat
     8461 gccgtctgaa aacgctgccg tttgcggaca agctcgtcat tcatgaaggc gacgtgttgc
     8521 agtttgattt caacggcatc gcaggcaaaa agaaaatcgt cggcaacctg ccgtacaaca
     8581 tttccacgcc gcttttgttc aagctggcgg aggtggcgga cgatgtcgtc gatatgcact
     8641 ttatgctgca aaaagaagtg gtcgagcgta tggttgccgc gccgaaaagc aacgactacg
     8701 gccgcttggg cgtgatgttg caatatttct tcgatatgga aatgctgatt gacgtgccgc
     8761 ccgaatcgtt cgaccccgcg ccgaaagtcg attccgccgt cgtccgcatg attccggtga
     8821 aacaccgcat cggcagggcg gacgatttcg agtatttcgc caaactcgtg aaactcgcct
     8881 tccaccaacg ccgcaaaacc atacgcaaca atctgaaaga gcttgcaggc gacgatgatt
     8941 tgcaggcagt cgacatcaat ccgcaagacc gtgccgaaca tatcgcgccg gagaaatatg
     9001 tggcgttgag caattatcta gcgggcaagg ccgtctgaaa agggagtaag ccgatgacga
     9061 aatacctgct tttacccctg atactgccgc ttgcagcctg cctgaccaat ccgaacgacc
     9121 gtttttttga cggcagacgc tacacgcttc atcatatgga gctcccgtaa cgacaacctg
     9181 caacggcaaa tgccgtctga aagaaaagac atgatcaaat tcaaaaacgt acacaaacat
     9241 ttcaaagacc tgcacgtcat caacggcgtg aacttggaaa tcaaaaaagg cgaagtggtc
     9301 gtcgtctgcg gaccttcggg cagcggcaaa tccacactca tccgcaccgt caaccagctt
     9361 gaaagcatcg aaagcggcga gatttgggtg gacggtgtca acgttgccga cccaaaaacc
     9421 gatttgaaca aaatccgcga agaagtcggc tttgtgtttc aaagtttcaa cctctacccg
     9481 cacctgaccg tgttggaaaa cattaccctc gcaccgatga aggttaaagg tcaaaatgcg
     9541 gaacaggcag agaaaaaagc gatggaactt ttggaacgcg tcggactggc acacaaaaaa
     9601 gacgccttcc cttcccaact ttccggcggc cagcagcaac gcgtggcgat tgcacgcggt
     9661 ttggcgatgg aaccgcgcgt aatgttgttt gacgaaccga cctccgcact cgaccccgaa
     9721 atggtcggcg aagtgttgaa agtgatgaaa gacttggcgg aaagcggtat gacgatgatg
     9781 tgcgtaaccc acgaaatggg ttttgcccgc gaagttgccg accgagtgct ttttgtcgat
     9841 aaagggcaaa tcctcgaaga cgaaacgccc gaagcgtttt ttaccaaccc gaaacacgaa
     9901 cgcgccaaac aattcctgca acaggtcatg acccactgat gctatgccgt ctgaaaaccg
     9961 aacgttcaga cggcatagcc cgacaccgca agatgattga gaaagaaaac catgtatttt
    10021 gttgaccgca ccgccgtcgt cctcaagccg accgtccgct ttttggaatg gctcaaaagc
    10081 accgacgaaa atatgcccga cctgaccata gaacagcttc gtgccaactg ttccgtcttc
    10141 ctcgttccgc agttcgacga accggaagcc gtcgtttcct atttcgacga acgctaccgc
    10201 cagattttcg aggcggagct ggcaggttgg gacatcgata aggataaatg gccgcaagat
    10261 atggggctga aggcgttttg ggaatttttc gacatcgaaa tccacgatat ggtgttggac
    10321 atggaagaag cggaattgaa cattaccccc gtgttcgaca acatgatgta aggctgtgcg
    10381 cgcctttcag acggcattga ggccgtcccg catcctgaac atcctgactg tatcgcttca
    10441 cgccgcctcc cttgccgtct gcctgacttg gttttacggc tggatgatgt ggttcggttt
    10501 ggcggctttg gtggcaagtt acgcatactc gctcaggata acgaatctga aacaccgcca
    10561 tgccataacc gccattacca tagaccgcga cggacaggcg gaaatcgtat ccggcaaaga
    10621 caaaacagcg gcaacacttg cgggcagcag tatggttacg ccttatgcct tattcttaca
    10681 atgggacaca ggcggcacaa ccgtcagcca atgcatcata cccgacatga cggacaaaga
    10741 atcctaccgg cggctgaaag tctggatact ttggcggcag ccgaaaaaaa ccgccgaaac
    10801 cgatacacca agctgatttc agacggcatt tgaacacgga acactcaatg aaaacactgc
    10861 aagactggct ctcgcatttg gaaaccgccc acagcggcgg cttgatcgat atgggcttgg
    10921 aacgcgtagg cgaagtgaaa aaacggatga agctcacgcc gcaatgcccc gtcgtcgttg
    10981 tcgcgggaac aaacggcaaa ggttcggtct gcgcctacct gacacagatt tacaaacagg
    11041 cgggctacaa aaccggcaca ctgaccagcc cgcatttatt gcgttacaac gaacgcatcg
    11101 ccatcaacgc cgaacccgtt tcggacgata cgattattgc ctctttcgag cgcatcgaag
    11161 cggcgcgcag cgaaatatcg ctgacttatt ttgaattcaa tacgttggcg gccgtcgaca
    11221 tcttcatgcg cgaacaggtt gacgtgatga tattggaagt cggcttgggc ggacgcttgg
    11281 acgcggtcaa cgcgtttgac ggcgattgcg cggtggttac cagcgtggat ttggaccatc
    11341 aggcattttt gggcgatacg gtcgagcagg tcggctttga aaaagcaggc gtgttccgta
    11401 gcggcaaacc cgccatctgc ggtcaaaacc ccgcgcccaa atcgttgctt tcacacgccg
    11461 aagccatagg cgcgaaactg ctgatggtgc agcgcgattt cgagtttcac gcgctggaaa
    11521 acatccaatg gaactaccac ttccgaccgc agcattcaga cagccccgct cgcaaccgta
    11581 attccctgcc cttccccgca ttgcgcggcg cataccaact ttccaatgcc gcctgtgcgc
    11641 tgaccgtgtt ggaatgtttg aacgacaaat tgccggtgga catcggtgca atcaaacgcg
    11701 gattgctgct ggttgaaaat cccggacgct tccaagtcct gcccggccgg ccgctgaccg
    11761 ttttggacgt cggacacaat ccccacgctg cccgcgccct gcgccgcagc ctgattaatt
    11821 tggcgtttgc gcaaaaacgc accgccgtgt tcagtatgct gtccgacaaa gacatagacg
    11881 gcgtgttgga aattgtcaaa gaccagtttg acgaatggta tatcgcacct ttggacatgc
    11941 cgcgcggcat gacgctcgag agcctgcaaa acaaactgca cgaacacagc atcgaaaaca
    12001 tacagacatt cgccgccgtc cgcgaagcat accgggcggc agcctcaaaa gcgggcgaga
    12061 acgacagaat cgtcgttttc ggctcatttc ataccgttgc cgatgtcatg tcggtacttt
    12121 aaaaggaaac ttctatgtcc gaaaacaaac aaaacgaagt cctgagcggt tacgaacaac
    12181 tcaaacggcg caaccgccgc cgcctcgtaa cggcaagttg cttggttgcc gcctcctgca
    12241 tcctgctggc agccgccctc agttccggcc ctgccgaaca gactgccggc gaaacaagcg
    12301 gcgtagaaaa caaagcggca ggtgcggcac aaacccctgc cttgaaatcc gccgccgaca
    12361 aaccgcagga cttggcaggc gaagacaagc cttctgccgc cgacagcgaa atcagcgagc
    12421 ctgaaaacgt aggcgcgccg ctggtgctga ttaacgagcg cctcgaagac agcaacatca
    12481 aaggtttgga agcatccgag aaactgcaac aggcagaaac cgccaaaacc gcaccgaagc
    12541 aggcaaaaca acgcgctgcc gaaaaagtgc cggcaactgc cgacagtacg gatacggtag
    12601 cgtttgaaaa accgaaacgc actgccgaaa caaaaccgca aaaagcggaa cgcactgcca
    12661 aagccaagcc caaagccaaa gaaaccaaaa ccgccgaaaa agttgccgac aaaccgaaaa
    12721 ctgccgccga aaaaaccaaa ccggatacgg caaaatccga cagcgcggta aaagaagcga
    12781 aaaaagccga caaggctgaa agcaaaaaaa cagccgaaaa agaccgttcg gacggcaaaa
    12841 aacacgaaac ggcacaaaaa accgacaaag cggacaagac caaaaccgcc gagaaggaaa
    12901 aatccggtaa aaaagccgcc attcaggcag gttatgccga aaaagaacgc gccttaagcc
    12961 tccagcgcaa aatgaaggcg gcgggtatcg attcgaccat caccgaaatt atgaccgaca
    13021 acggcaaagt ttaccgcgtc aaatcaagca actataaaaa cgcaagggat gccgaacgcg
    13081 atttgaacaa attgcgcgta cacggtatcg ccggtcaggt aacgaatgaa tagcctcccc
    13141 attgccgacc tcctcgtctc cgccgtcatc gccgcctgca tcgtcatttc cacgatgcgc
    13201 ggcgtgatta cggaggcagg ctcaatggcg gcatgggtgg tttccttctt tttcgccaaa
    13261 ctctttgccg cacccttcgc cgacatcgcc tttgcatcgt tccaaccccg cctgtttgca
    13321 ttggctctgt cgttcatttc cctattcgtc attgcctgtc tgatccagaa aatactccgc
    13381 tcgctgctga ccggggcagt ttcggcggtc ggtttgggct ttgccaaccg cattttgggc
    13441 ggcgtattcg gtgcattgaa aggcattttg attattaccc tgctggtcat gctcgcttca
    13501 aaaaccgacc tgcccgatac cgaagaatgg cggcaatctt acacactgcc gtttttcgta
    13561 tcgctttccg aagccgtgtt gaaccatagc ggcggcacgg cggaaactcc ggaagacgat
    13621 tgattgccat acggatattt cagacggcat tctcttttat gccgtctgaa atgttttttc
    13681 ctttcctcag ccatatcac
//
LOCUS       DAXHMX010000043        13691 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHMX010000043 DAXHMX010000000
VERSION     DAXHMX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.043"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="ACFM4S_000917"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="ACFM4S_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6050632.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKARLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="ACFM4S_000918"
     CDS             complement(1643..1798)
                     /gene="rpmG"
                     /locus_tag="ACFM4S_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6050633.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1830..2063)
                     /gene="rpmB"
                     /locus_tag="ACFM4S_000919"
     CDS             complement(1830..2063)
                     /gene="rpmB"
                     /locus_tag="ACFM4S_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6050634.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2313..3839)
                     /gene="farB"
                     /locus_tag="ACFM4S_000920"
     CDS             complement(2313..3839)
                     /gene="farB"
                     /locus_tag="ACFM4S_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6050635.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSSGGGGH
                     "
     gene            complement(3863..5035)
                     /gene="farA"
                     /locus_tag="ACFM4S_000921"
     CDS             complement(3863..5035)
                     /gene="farA"
                     /locus_tag="ACFM4S_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC6050636.1"
                     /translation="MATHADETTIQNRQEANSAKAKRKRCLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            complement(5079..5438)
                     /locus_tag="ACFM4S_000922"
     CDS             complement(5079..5438)
                     /locus_tag="ACFM4S_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6050637.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICAPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     regulatory      5653..5697
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5702..6175
                     /gene="queF"
                     /locus_tag="ACFM4S_000923"
     CDS             5702..6175
                     /gene="queF"
                     /locus_tag="ACFM4S_000923"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6050638.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            6252..6938
                     /locus_tag="ACFM4S_000924"
     CDS             6252..6938
                     /locus_tag="ACFM4S_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6050639.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(7441..8733)
                     /locus_tag="ACFM4S_000925"
     CDS             complement(7441..8733)
                     /locus_tag="ACFM4S_000925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6050640.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGKVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            8811..9779
                     /locus_tag="ACFM4S_000926"
     CDS             8811..9779
                     /locus_tag="ACFM4S_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050641.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDGTNGGAQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(9980..11446)
                     /locus_tag="ACFM4S_000927"
     CDS             complement(9980..11446)
                     /locus_tag="ACFM4S_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6050642.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKSPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(11688..13691)
                     /locus_tag="ACFM4S_000928"
                     /pseudo
     CDS             complement(11688..13691)
                     /locus_tag="ACFM4S_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         3225 a         3979 c         3262 g         3225 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggtccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtctgg gtatgtggga caaaattccc gaaaatgaaa tttacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 cgaggcgcgc ggcgaacctg ccgactgttt gggctatctt atttccaacc acaatatccg
      541 acgcgctgcc tacgaagtcg tatcccaact cgacaacatt accattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgcttc tcgcaaaccc gtcgacaact
      721 cggtatctcc tccgatatgc acgattacag ccgaaccatg ttcgtctgcc gtatgaaaca
      781 taccctttcc aaccaacata ccgcttacga atgcttccat tacggacgca ccatcgcact
      841 gctgccgctg gaagagcacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac agctcaaggc
      961 caggcttggc gacatggaac tggtcagcag catccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc atgcccagcc gctttatcac ggaaccaata tgatgctgaa
     1261 actgtttacc aatgagaccg cgccggcaaa actgctgcgc ggtttggtat tgcgtgccgg
     1321 caacaacttc ccgccgctga aaaaactgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt atagtggatt aacaaaaatc
     1441 aggacaaggc gacgaagctg cagacagtac aaatagtacg gaaccgattc acttggtgct
     1501 tcagcacctt agagaatcgt tctctatcct tggcagacgg tttgcagcag attcgcatac
     1561 ggcagatgtt tcaagcggaa aagaaacacg ataaaaataa aaaagcctcc gaacagtcgg
     1621 aggctttgct ttcaaactga aattatttca gtttggtttc tttgtacact acgtgtttgc
     1681 gggcaaccgg atcgaatttt ttaatttcca gtttgccggg catagtgcgt ttgtttttgg
     1741 tagtggtgta gaagtgacca gtacctgcac tggattccag tttgatttta tcgcgcattg
     1801 cagtaatcct taattaaata gtgtttaaat taagcttcgc cgcgagcacg caaatcagcc
     1861 aatacgacat caatgcctac tttgtcgatg gtacgcagtg cagcgttgga aacgcgcagg
     1921 cgaacccagc ggttttcact ttctacccaa aaacgacgtg attgcaagtt gggcaaaaaa
     1981 cggcgtttgg ttttgttgtt ggcgtgcgat acgttgttgc cggacatcgg gcgtttgccg
     2041 gtcactttgc aaactcgtgc cataatcagt ctccaaactt ctaaaatagt aaaacgcgat
     2101 ttataccacg aaaaacagtg ttagttcaag tgttttaaag aaaaacggct gttttgcgcg
     2161 gataatcctt ccgattgtcg catctgcgga acgtttgcag gtgcgaaaca gatggggcgc
     2221 gattatagcg gatttcggga actgctgcat tcaaaatgcc gtccgaacat atttccagta
     2281 ttttcagacg gcatttcaag ttttcaaatc cctcaatgtc caccgccgcc actgctgtgg
     2341 aacggcggtt ttgccagcca tatgacgggt atcatgataa tgaataacaa gctgcctgcc
     2401 ataaagattt cattcgatcc gatgatgaag ccctgctggg taatggtatt gttgatgata
     2461 cctagggttt gaatgtcgga aatgccttgt tgggacaaat gcgccgccgt ttcgtgcaat
     2521 gttgcggaat agggcgtgat gtgttcggca aagcgcgtgt ggtgcaacgc ttcgcgccgt
     2581 tcccacaggg tgctgacgac ggatacgccg acaccgccca tcagcacgcg caggaaattc
     2641 gacaggctgc cggcggcggc aatctgcccg cccttcatgt gcgacagtgt gatggtagtc
     2701 agcggcagga agaacatggc aacgcctact ccctgccaaa actgcggcca gatgacgttg
     2761 ccgatatcca tgtcggcata aaaatcggtg cgccaataga aagtgaaggc aaaggtcagg
     2821 aagctggcgg ttacgagcag gcgcatatcg attttattgc cgaacctgcc gattaacgga
     2881 gacaggaaga cgggcaggat gccgacgggc gcggcggcaa gccccgccca cgtggaggta
     2941 tagcccaggt tggtctgcaa cactaacggc agcagggtca gcgtccccat atacaccata
     3001 aaacccaatg atgtggcaat gatgccgacg gtaaaattcc gatctttaaa cagcgataaa
     3061 tcgacaatcg gatatttttc tcccaattcc caaacaataa aatacgacaa gcacaccagt
     3121 gcgactacgc ccaaggtaat gatttctcca gaggcgaacc agtcgagttc cttacccctg
     3181 tccaacatca tctgtaacgc gccgataccg actaccatca atgtaagccc gacatagtcg
     3241 gtcggcattt taacggtttc cgtttcccga catttcaaat gtttccatgt aatccatgcc
     3301 gatatgatac cgatagggat attaatgaag aaaatccaac cccaatgcca gtttccggaa
     3361 atccagccgc cgagtatcgg cccgagaaca ggggcgacaa cgacggtcat tgcccacaat
     3421 gccagtgcca gcgtccgttt tgcgggcgga taggatgcca ttaacaggct ttgcgacaag
     3481 ggaatcagcg gcccggcgat aaagccctgc aagatgcgga aaacaaccag cgactgaagg
     3541 ttgggggcaa taccgcacag ccacgatgtg atgacgaaac cgacagcggc ggcggtaaac
     3601 aatttgacct cgccgatacg ttttgccaaa aagcccgtta acggcacgga aacggcgttt
     3661 gccacagcaa aagaagtaat gacccacgtc ccctgcgtgg ttgccgcacc gaggttgccg
     3721 gcgatgacgg gaacggcgac attggcgata gtcgtatcta aaacttccat aaatacggca
     3781 agccccaaag acagcgtaac ccacgccaat gccgcaccct taagcggtgg ataatccatt
     3841 gtgtcctccg tttcagacgg catcagggcg cggattgccc gaggatttca tcgaccgtcc
     3901 gatcgacttc cgaccagtcg gtactttcca tttccggcaa tgccgcaccc ggcgtttttg
     3961 aaacaggcgc gcctgcggcg gaagtatcca ctttaaccgt catcgacaaa ccgatacgca
     4021 acggatgcct gtccacatct tcgcggttca gcacgatacg gacggggacg cgctgcacca
     4081 ctttaatcca gttgcccgtt gcgttttgcg ccggaatcag cgaaaacgcg ctgcccgtac
     4141 ctgccgaaaa acctgccacc ctgccgcgat aaacaatttg tttgccgtac aaatcggaca
     4201 ccagctcggc aggctgtccg attttcatat gccgcaactg cgtctcttta aaattagcat
     4261 ccacccacac atccgacagc ggcaccaccg ccatcagcgg cgcgcctgcc gccacctgct
     4321 gcccgacctg caccgaacgc ttcgccacct gaccgtccgc cggcgcgcgg atttgcgtcc
     4381 gctgaaggtt caaccacgca tctttcaacc tgccgattgc cgtctgaacc gccggctgtt
     4441 cgcgcaaaga aacctgaccg cccaaagccg cacgcgccga agattcttcc gccaaagccg
     4501 ccttaactgc cgcctgagcc tgagacactg ccgcacgggc atgtgccagc tcttcggcgg
     4561 acaccgcgcc cgattccgcc aaagcagacc ggcggcgcaa atcatcctgc gcgcgtgcca
     4621 aatccgcccg gcgcaaggca acctgcgccc ccgcctgcga agtggcggca ttttgccggc
     4681 ggttttgccg caccgcctga atcagctcgt tttttgcccg ctcgtaagcc agcacatcat
     4741 tatcgtcgtc caataccgcc agcacgtcgc cttttttcac ggcatccgta tcgtcgtgca
     4801 aaaccttccg caccgtaccg cccttttgcg gcgtaacctg aaccacgcgt ccggcaacat
     4861 aagcgtcttc cgtttcctct tcgtgctgcc accataaaaa aaacgccgac ccggcggctg
     4921 cggcggcaag cgcgaacagc agcgtcaatg ccgtcaggca gcgtttgcgt ttggcttttg
     4981 cgctattggc ttcttgtctg ttttgaattg tcgtttcatc tgcatgtgtt gccattattc
     5041 caattccaaa tacttctaat actatgtttt tgatttaatt agcactgata atcttcaaat
     5101 tcccacgaaa aaaaacgaag taaataagtc aatgactttt cccaagtttc ttttgaacat
     5161 tctttaagaa ttttctcaat ttccgattta ataacagaat gattaaattc attcataatc
     5221 atcatacccg ctccccattt aaccctttga ttttggaaac aattatgcaa aatccattta
     5281 ggagcgcata tgcgaacaga aaatatatct gcagcatcac tatcatcagt tcctatgtct
     5341 aaatcaattc ccacacaaaa attgtctttg atttcgggaa cgaaatcttc aaaggcacaa
     5401 tcgtaaagat tgatggcttt caattctagg ttaatcattt tatattcaaa ataaataaaa
     5461 ccaattaaag attggttagg attctatacc ctggcaataa ctcaatcaag ttaatgattt
     5521 agaatgaata ttcaaataaa ctgtatcagc ccggcctcac agaactattt atatccacac
     5581 atcaccgagt aaagcaatta cccatactaa ccgaagaaaa ctacacaaat accgccactt
     5641 atattacaat cgccgcccgt ggttcgaaaa cctcccacat taaaaaacta aggaaaccct
     5701 catgtcccgc aacaacgaag agctgcaagg catctctctt ttgggcaatc aaaaaaccca
     5761 atatccgacc ggctacgcgc ctgaaattct cgaagcattc gacaacaaac atcccgacaa
     5821 cgactatttc gtcaaattcg tctgtcccga gttcaccagc ctctgcccca tgaccgggca
     5881 gcccgacttc gccaccatct acatccgcta cattccgcac atcaaaatgg tggaaagcaa
     5941 atccctgaaa ctctacctct tcagcttccg caaccacggc gattttcatg aagactgcgt
     6001 caacatcatc atgaaagacc tcattgccct gatggatccg aaatacatcg aagtattcgg
     6061 cgagttcaca ccgcgcggcg gcatcgccat tcatcctttc gccaattacg gcaaagcagg
     6121 caccgagttt gaagcattgg cgcgtaaacg cctgtttgag cacgacgcac aataaaccat
     6181 ccttcccata tttcagacgg cattccttcg atacgatatg ccgtctgaac gcctttccga
     6241 aaggtttttt tatgtacgcc tttaccgacg cacagcaaca gaaggcactc ttccggctgg
     6301 tgctttttca tatcctcatc atcgccgcca gcaactatct ggtgcagttc cccttccaaa
     6361 ttttcggcat ccacaccact tggggcgcat tttcctttcc cttcatcttc cttgccaccg
     6421 acctgaccgt ccgcattttc ggttcgcact tggcacggcg gattatcttt tgggtgatgt
     6481 tccccgccct tttgctttcc tacgtctttt ccgttttgtt ccacaacggc agttggacag
     6541 gcttgggcgc gctgtccgaa ttcaacacct ttgtcggacg catcgcctta gccagctttg
     6601 ccgcctatgc gctcggacaa atccttgata tttttgtatt caacaaatta cgccgtctga
     6661 aagcgtggtg gattgcccca accgcatcaa ccgtcatcgg caacgctttg gatacgctgg
     6721 tatttttcgc cgttgccttt tacgccagca gcgatggatt tatggcggca aactggcagg
     6781 gcatcgcctt ggtcgattac ctgttcaaac tcaccgtctg cggtctgttt ttcctgcccg
     6841 cctacggcgt gattctgaat ctgctgacga aaaaactgac gaccctgcaa accaaacagg
     6901 cgcaagaccg ccccgcgccc tcgctgcaaa atccgtaaaa cagccctgcc ggaaatcggg
     6961 acggttgggt tttcttttat ttgcaaacac tgtcggaacg gataaatccg gattcccgcc
     7021 cacttcgtca ttcccgcgaa agcgggaatc cggtgcgttg ggtttcagtc atttccgata
     7081 aactcctgcc gcattgtatt tctagattcc cgctttcgcg ggaatgacgg cggatgggtt
     7141 tccgtaccgt ggtgtccgga ttcccgacgg tacgggaatg acggcagtgc ttccttggct
     7201 tagttttatt ttaatcgact gtatggtgga ttaacaaaaa tcaggacaag gcaacgaagc
     7261 cgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcatc ttagagaatc
     7321 gttctctttg agctaaggcg aggcaacgac gtactggttt ttgttaatcc actataaatg
     7381 ccgtctgaaa acctgtttgc agattttcag acggcattgt tttcgtcgcc ggttcaaact
     7441 tcaatccgat tgcgacacgg taaccggtat gccgcgcaag cgcgcaagca gcgcgtccgc
     7501 cttctgtttc tgcgcggcaa acgccgcctt gtccgcctgc gtcaattcgg gtgtcggcaa
     7561 tgcgaccgaa acaggattga cgggctgccc gttgatgcgt gcctcgtaat gcaggtgcgg
     7621 cccggtcgaa cgccctgtcg aaccgacaaa accgatgacc tcgccgccgc gcacattgcc
     7681 ttgtgcctgc gaaaacgcgc tcaagtgcgc gtacagcgtt tccacaccgt tggcgtggcg
     7741 tatcatcacc gcgttgccgt atccgccctt ccggccttta aaggtaatca cgccgtcggc
     7801 ggaagccctg accggcgttc cctgcggtgc ggcataatcg atgcccgtgt gcagccgcca
     7861 tgtgtgcagg atggggtgca tacggtagcc gaacggcgaa gaaatgcgcg tatagaccag
     7921 cggctcgatg ttgaagccgc ctttttcctg caacaccttg ccgtcttcat cataataatt
     7981 gccgccccct ccgccttcct tgtccgaacg gtaatagaac gcctgatgcc ttgtgccgcc
     8041 cttaacgact tcagccgcca aaatatcgcc cgccgccacc tgctgcccgt ggaaatacag
     8101 gctgtcgtac atcaggcgca cggcatcgcc ttccttcaaa ccgtcaaggc tgaagcggcc
     8161 ggcgaaaatc ccgcttaagg attcgcggat ttcgacgggc acttccgccc gcgccagcga
     8221 accgcgcgcc gacgttttga ccacgaccga acgcagcgtc ggcaaaacct tcatatccgc
     8281 ctcagaagcc gaccgccgcc atatgccgcc tttcttttcc aaagcgacca gattgcgctc
     8341 gccgtcttcg tcggtaaaaa actgcacttc gcgcgcgccg ccgtcgccgc cgaccaaaac
     8401 atgaaccgac tggtcggcac gcaaatgccg caaatcggct tcgccgccat atttttccgt
     8461 gattcgggca atctcgtccc gcgccatacc cgaacgcgcc agcacgtccg ccagcgagtc
     8521 gcccggctgc accgcctcct gcacccaata tgccgtctga acgccgctgc cgccccaaga
     8581 cagcggcggc agattttgtt ccacgcgctg cggtctgacg cgctccgtcc tctctgtcga
     8641 agcaatgtat gccgccgaca ccaaaataat cgaaacggca agcgcacgca gcgcgtattt
     8701 ccgatgtttt gccgaaagtg ggaagacagc catattgttc ctttttgacg gcgtttgcgc
     8761 cgaatcctct ataatgttgc gcctgacctg catttctgaa acgtcaaacg atgaaactca
     8821 ccctcgccct cccctcgctc aatttggatg aagacgaaat ccgaatacct ctatgcctgc
     8881 ccgccttcaa caaaatcctg caatacggct cgctgcaccg ccaaagctgc acggcttcgg
     8941 cattttacgc gcgttatttg tggtgcggca gactggcgga acgcgcggcg caaagcctga
     9001 atatgccgtc tgaagccgtc gcgctcgcca cgcccgtctg gcaaaagatg gggatgcatc
     9061 aggcaaatgt gctaacggcg gaatatctga acgtcggtac ggatgaagcg gaacggctct
     9121 gccgcgacct gtcggcgttt tacggcgaca ttccatggcg tttcgttccc gtcctgcccg
     9181 aattatggct ggtttccctt ccttgcgcgt accgctgggg cgcgaagccc gttttggact
     9241 tgggcggatt gttgggcgcg gacgatcagc cggacggaga agatgcgttg gaatggctgc
     9301 gcgtccaaac cgaaatccaa atgtggctgg cggcgcaccc cgtcaaccat aaccgcaaaa
     9361 aacgcgggct gcccgagttg aacggcttat ggctttggga cggcacaaac ggcggcgcgc
     9421 aaggcggcac acttttcgcc gatacggttt ggagccgttt ccaccccgac cgccgcgcat
     9481 tgcccgacaa cttccgcgcc tatgaagaaa cggcggcaca cctgcctgac acgcaccata
     9541 tcctgtttat ggacgatttg cgcctgaccg ccctgacagg ggacagggag cggtatgcgg
     9601 caatattgca gcagtgggaa gaacgctggt tcgcaccgct ttacgaagcc gtccgtaccg
     9661 gcaaaatcaa acggctggac atcaccacgg acggacaaca cggcggtacg ctgaccttca
     9721 aacccgccga caggtggaaa ttttggcgcg gcgcgaaaac ctttgacgga atctggtgaa
     9781 cccgcctgcc gcaaccgcga caatgccgtc tgaagcccga atcgggtttc agacggcatt
     9841 ttacctcgca tcgccgttat gccggaaaaa ccggaatgcg tttccgcaca cgcggcggca
     9901 cgcaaagcac cgcccgaaag ccgccgatgc ccgccgtgat accgccgccg gcagggtttc
     9961 ctccgaaaag gaacagcaat cagaacgttt tattaaactc gacaaacgcc cgatttttct
    10021 catattcgtt gaacacatcg ttactccacg tttcgcggtg cgacagcgtc aggcgcggcg
    10081 tgatgccttt gaaatgcaat gcccggtgcc aaaggctcaa ggatgtgttc aattctttat
    10141 ccctgcgcct ttccccttta aaaccgctga aaaagccggg tttttcataa tgccgtttcg
    10201 ccgcgccgag gcgcaacagc gaagacaggc cgctgccgcc ccattcctgc ccccaggcaa
    10261 agcgcaggcc gtaacggttg aaattgtcgc cccggtcggc ggggttgcgc tcgcggtaaa
    10321 aatccaaacc gcccatccaa tattggcgcg cattccggta aaacaccagc gaattggaaa
    10381 tttgcaaatg ggtattgtcg gaacgcgccc ggcgcgtatt cttcaaacgc ccccactccg
    10441 ccgaagacaa cgtttgccat ttcggggttt gccaacggtt gaaataaagg cgtgcgccgt
    10501 tggtgtaaga ataagcgtcg ttgccgtagg tgcggcgttc gtggaacact gccagcccgg
    10561 catctttgcg ccggtcggca aaaccgatgc cgccggaaac gcctgccgtc atatcgttga
    10621 atttcttatt ccccggataa accctgccgg acacgtcgcc gcccgccgtc gtgtaccagc
    10681 cgtttttcag cgaccatttt ttctccgcgc cgagccggta attgaccgcc gtgccgtcca
    10741 cctgtttcgg gaaagtccat ttgccgtact gctgccgttt cggggcttgg ttgatattgt
    10801 gttcgcgggt aacgctgaag ccgccgttta ccttccacgc atcgcgttcg cgcaatgcct
    10861 tgcggtacag ctcgacctgc tccatcagct gcggcggcag gttttccgcc ttcaggcggt
    10921 cgaactggtc tgccgccgcc tcgttctgcc tgtcttcaaa caatgctgcc gccaaacgca
    10981 tacggacggc gggcgcgtcg ggttgggcgg caatcaattc ccggtaatgg gaaatcgcct
    11041 ccttcaccct accgtctgcc tgcgccaaaa tcccttgtgc ataaagtgcc aacatcttat
    11101 cctgctgcgc ctgttgtagg taaatcggca aaataacgcg gataccggca atattgtttg
    11161 agaccactgc ggaatacatc gcgcgggaca acaattcggg acttttcagc agggtttctc
    11221 cgtcaatctg caaaactttt cctttttccc gcacctgccc cggtaccttc tccctgtcga
    11281 ttggtttgac ctccgcttca tgaagcctga actcgggacg gcttctcaaa tccggttcgc
    11341 gcggtgtttc ttcggcatat gccgcacttg ccaataaagg cagcagcagc atccatctgt
    11401 ttcgtgcagg cataaatgtc ttcccacaaa aataaaaaat aaccattatc aatcggcgga
    11461 ttgtatcagt tttttatgcc gctgttccaa accctgccgc ccgatgccgc gacaatgccg
    11521 tctgaagcca gaatcgggtt tcaaacggcg gcaaaaaacc ggcggttttc cgccggtttc
    11581 atcattatgc cccgcccttc ggggcagcag ggttcggatt ggtttgcggg gcggcgtgcg
    11641 gtgccgtgcc ggtgtgccgc ctgtacgtca aaacatttcc cgacgcatca aaacagccat
    11701 ttgagcgaca atgcggcttc tttgtcgccg tccgtccttt tgccgtatcc gatgcgtgcg
    11761 gacatatgac ctttccaacc ggcttcaatg ccgaaccgcc cttcgagcgc cgtcctgcct
    11821 gccagcgtct gtttttcgcc gtccatttcc acgccgaaag attttgacct gtgcaaaaca
    11881 ttaaaagcgg caaaaggctg aagattgaca ccattacgca aagcaaaacg gttttttgcc
    11941 cgaatgccgg cgcggctttg ccactgaccg ctgccgagca gtccgacccc cgtcccctcg
    12001 ctgtcggtaa agccgccgtt tacgcccaag taggtaaact gcgcctgcgg ttgcaggtaa
    12061 aaccgcacat tattgccttt tccgacaact ccttccgcca caagcgtgtt gtagccgcct
    12121 tcgacagaag ccgtccaacc tttggttttg tagcgttccg cacggttttc atcattgatg
    12181 cggtgtttga aacgttggta ttgcaaccag ccgtccaaat acgcacccgt ttgtttatcg
    12241 cgcaactgat gccacgcagc ataaacaccc ccgccataac catgcaaatc actgcctgcc
    12301 gcaccgcctt tgctgttaac tgatgcgtgc tggccgaccc tgccgcccat cacgccgatt
    12361 gccagccggc tgccttcatt ttgccgtaca aacacctcgc cgccgatttg cacgcctttg
    12421 cgccgcccgt ccgcagccgc gccaccccgt atattttgac gggacggatc ggcggcatac
    12481 acgtcgtcgc ccctgtcggc ggcacgcagc ccaaacaaag tatttgccgc ataagcctgt
    12541 tgggcatata aaaccgcttc cgcctcgggg atagccaaac gctgcacgcc caagcaaaat
    12601 ccgccttcgc attgcttgag caataattcg tacagtccct gccggtaatt ttgtttaaac
    12661 gcaaacgaac cggaatccgc cgtttttgcc gcaatcagtt tgcgcccgtc gagcgttttc
    12721 agattttccg gccggcgttc caaaaccacg ccggattgtc cgctgacttt gtcttcaaag
    12781 gtcagggcgg gttcgtccga ctcccgcacg tcgtagccga acaggaaatc cgcgccgccc
    12841 gcataatcct tatggacggt cagtttttca tctttcgtgt tcaggcggat gcgggcatta
    12901 tcggaagttt ccagcgtatg gatgtcggaa tggcggcgcg gttcccaaag ggaatctttc
    12961 agcgtcatct tggtcagttc caacacctgt ccagcgacgt gggatttgtt ttcgattttg
    13021 acgtgtaatt ttttattgaa gatgttgtcg agattggtcg gtgttgtatt actatgagga
    13081 gtgagtacag cttcttctat aattggatca ataacttttc taaatttatt aattttattt
    13141 ctaggtattc catattgttt tattatcgct tcctcaacta tttttttatt tttttcccca
    13201 atcttcgggt cttgttttat ataaatcctt taattgatcc ttaaccgcct gctttaaatc
    13261 agctccgtaa ttctctgtaa catatttctt aacaacgggc atattatcga cggcgaggat
    13321 gccgctattg tagtttttac ccgtgatatt gatttcgtca tacaaggctt cagatgaatt
    13381 tggatcggtt ttaccgctgg aaaaataatg ggaaacgccg tttgccggct tgtctgaaaa
    13441 tgaaaaatcg gcagcatcgc caatatttgt agaacgagta tccgaaccgg gctgataact
    13501 gaaagcaccg aagcaatcaa aagacacact gcacgggccg cgccaaacgt gggaatttct
    13561 gtcttccgcc gcttctaaaa taaatttgtt tttaatctcg gcttctttga cgggatcgta
    13621 cgcatccgaa cctcaaacat atggcgtgca aaacagcgat aaaacagata atgttaatag
    13681 attaacccga c
//
LOCUS       DAXHMX010000044        13525 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHMX010000044 DAXHMX010000000
VERSION     DAXHMX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.044"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            495..1093
                     /locus_tag="ACFM4S_000929"
                     /pseudo
     CDS             495..1093
                     /locus_tag="ACFM4S_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1082..1429)
                     /locus_tag="ACFM4S_000930"
                     /pseudo
     CDS             complement(1082..1429)
                     /locus_tag="ACFM4S_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            1740..2492
                     /locus_tag="ACFM4S_000931"
     CDS             1740..2492
                     /locus_tag="ACFM4S_000931"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6050643.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHMEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            2683..2758
                     /locus_tag="ACFM4S_000932"
     tRNA            2683..2758
                     /locus_tag="ACFM4S_000932"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2716..2718,aa:Asn,seq:gtt)
     gene            2889..3899
                     /gene="waaF"
                     /locus_tag="ACFM4S_000933"
     CDS             2889..3899
                     /gene="waaF"
                     /locus_tag="ACFM4S_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6050644.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDKAKIVSLHLECSPCFKRECPLGHTDCLNKLYPEKIVQAVEE
                     AV"
     gene            3954..4400
                     /gene="smpB"
                     /locus_tag="ACFM4S_000934"
     CDS             3954..4400
                     /gene="smpB"
                     /locus_tag="ACFM4S_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6050645.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            4831..6816
                     /locus_tag="ACFM4S_000935"
     CDS             4831..6816
                     /locus_tag="ACFM4S_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC6050646.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDNSKLTNEQIDRLKKLRDKSSEY
                     WKQETYLLKPTNGSSQNSLKVPALYSKTHQFKNINNSKKISFYDKEYTEDYLVGFARG
                     FGVEKRNGEEEKTLQQYFKDCVNTGNSNNDKCEQPSFENSGPRLIKSDIFALAPQIKN
                     SHINSEILSVGNYIEWLRPTLNKLPSWQEHLYSGLDPFHYIEVTDNSHVIGQTISLDE
                     FRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDLKDKVS
                     ETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYT
                     LSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWLR
                     VIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSVGLMSGQAEQRSTFRNP
                     DTDNLTTGNVKGFGAGIYATWHQLQDKQTGAYADSWVQYQRFRHRINTEDATERFTSK
                     GITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAHVNLLGSR
                     QLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEIDGERRVINNKTAIESQL
                     GVAVKIKSHLTLQASFNRQTSKYHQAKQGALNLQWTF"
     gene            complement(6997..8364)
                     /locus_tag="ACFM4S_000936"
     CDS             complement(6997..8364)
                     /locus_tag="ACFM4S_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6050647.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLIIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELVGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(8474..8785)
                     /locus_tag="ACFM4S_000937"
     CDS             complement(8474..8785)
                     /locus_tag="ACFM4S_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050648.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPATEAPAAEAPATEAPAAEAPAAEAAATEAPAAEAPAAEAAATEAPAAEAPAAEAA
                     K"
     gene            9259..9741
                     /locus_tag="ACFM4S_000938"
     CDS             9259..9741
                     /locus_tag="ACFM4S_000938"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6050649.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            9828..10640
                     /gene="hpnC"
                     /locus_tag="ACFM4S_000939"
     CDS             9828..10640
                     /gene="hpnC"
                     /locus_tag="ACFM4S_000939"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6050650.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(10711..11181)
                     /locus_tag="ACFM4S_000940"
     CDS             complement(10711..11181)
                     /locus_tag="ACFM4S_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6050651.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            11272..13113
                     /locus_tag="ACFM4S_000941"
     CDS             11272..13113
                     /locus_tag="ACFM4S_000941"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6050652.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         3313 a         2994 c         3770 g         3448 t
ORIGIN      
        1 acgccgacgg aatttcgctt cgcattaact gccttatttt gaacgcacgt caaacttaaa
       61 tttgcaatgc cgtctgaaaa gaagatgggc tgtcggatac ggatgtccga ccattttatt
      121 agacctaacg tgggtttgag ataacacatt gattttttat gtagagcgat ttatttcaac
      181 ggcaatccat tccctctctc gtgggagagg gttaggggag ggcatcgggg cggtaacgga
      241 aatattgtct gtatctgccc cgttttaccc tctccccaac cctcccccac aggggaggga
      301 gtcaagttgc aggcacgccg acggaatatg gttgaaaccc ggcccttctg ggcgatggga
      361 ttttggttta ttttttggga agtaacccct tccaaatcag ggtaaacagg gctgtgcctt
      421 atgtgcggcc ctgtgtgttg aaacattgag gaatataacc gtgccgtcaa attactttat
      481 atacgcgcgg acgattgggt gctttgggcg gcatcgtgca acatcatacc gccttgtacc
      541 gcagcgtggc ggtatatgaa ccggctgtgc ttatcgtgcg gcgcggaagt aagaagctgc
      601 ggcgggtgag gcggtcgcgt tggcaggcgg acagacgttt gacgttatta atattcccga
      661 tctagacggc ctctatcagg cgcaatggat tgctttcgaa caggaaaccg tagaaaggtt
      721 cgccgcacaa tacgggacgg cgcaggcagt gtgcgatgcg gtgaagctgc cgcaccccgg
      781 gcggatgggc gcggcgtttg attatgcggc ggcggtgctg acggatgaag aagtgccgca
      841 caatgcggcg gaggtcgcgc tttgcggcgt gttggcgtgg ttgcagcatg acggcatcgg
      901 ttttgccgtg tacgaaggcg tcaacctgat gcggcaaatc cgcaaactga ttaccgccga
      961 tatggctgcg gactggtcgt cggcgatgtt ggcgcagcgg cttaattgca gcgaggccgc
     1021 gttgcggcgg cggacatcgg cgggaatgtc ggcgaccacc cagtgcatcc agcccagtcc
     1081 ggtcggcgat gttggcgcag cggcttaatt gcagcgaggt cgcgttgcgg cggtggacat
     1141 cggcgggaat gtcggcgacc acccggtgca tccagcccag tccggtcggc gcgtctttat
     1201 cgtaaacggt caggacgaaa ctttttgtcc cggcgggcgg atttttccac gacagcgcgg
     1261 gcgaagcatt gccgcccgaa cagccaaatc cgtaaggcgc gctcaaaagc tggttttgcg
     1321 tgaagccgcc gtcttcggac gggttgtcga attgcagcgt gaatgcgccc ccggcaaagg
     1381 cagcggttga aaaagccagc agtacggcgg agagaatgcg ggtttgcata gacggttcct
     1441 ttgcttgatg aataagtgga ttcattatag gctgtcaacg gcggcaggaa tgtgccgaaa
     1501 cggttggatg atgtgccgga atgcgcaaat gcaaaaatcg tggtttcaga cgagcctttg
     1561 caggtcgtct gaagtcttta attcctttcc ctgaggcttt agaaatatcg aatatttagg
     1621 tattgccgca acctaaccct caagatttac gtcatagccc gttcctgctc cggcgtgcgg
     1681 gaatggcaaa aattggggat tttgccgcca aatccatgat agagatacgg agcaaacgaa
     1741 tgattaccct gccttcctta aacaatctgc cgtcaaaatg ggatgaaatc cgctattggc
     1801 tcgaaacccg ggtttgtgag tgcggcgtta tgccgcatcc caatttgacg gaatgcgaag
     1861 ccgggcagtt cgatcgggat ttttgggaca atatcggctg cgcgcctgaa gaatatgtcc
     1921 gtatccgccg agccatccgt ctgttggaag cgcgttatcc cgacagtttg aatgagctgg
     1981 tgtgtgccgc cattgccacg cctttgggcg agatgctggc ggtgttcgga aataagggat
     2041 tgtgtctgct ggagtttgtc ggacagaaac atatggagca ggaaattgcc gccgtccaaa
     2101 aagccttgcg cggacggttt gtgtttcggg aggatgagcg gacgcaactt ttgcggcagg
     2161 aattggactt atacttcaag ggtcgtctga aaacctttgc cacgcctttg gagcagattg
     2221 gcaccgagtt tcaaaagcag gcatgggacg cgctcttggc gattccttac ggcgaaacgc
     2281 gcagctacaa agagcaggcg cagcgtttgg gcaaccccaa cgccgtccgc gccgttgccg
     2341 ccgccaacgg gcagaacaag gtgtctatct tgattccctg ccaccgcgtc atcggcagcg
     2401 acggcaaact gaccggctac gcgggcgggt tgaaccgcaa acagttttta ctggctttgg
     2461 aacgcggcga agttcagacg gcattgtttt aagcggggga gggcagaacg gctcatcggg
     2521 cttaaatatg gtgtgatgac ggctgtggaa attcatacaa cggcaatcct tttaaggcat
     2581 cggtcttaat gtctttctga ttcatatgct tgtcgttttg tcgcccattt ttccaaaatt
     2641 aaggattgac acattttttc cggacgctat aatacgcccc attccccgat agctcagtcg
     2701 gtagagcgac ggactgttaa tccgcaggtc cctggttcga gcccaggtcg gggagccaga
     2761 attttgaagc ccgaacttga atgttcgggc tttttcatat ttgcacgtac ggcgttcgga
     2821 atgcggcaag ataggttttc caacggtttc gcggtaaaat tctgcctgtc tgatttcggt
     2881 ttcttgttat gtccatcaaa atcctgatta tttctcccag ttggataggc gactgcgtga
     2941 tgacccagcc cttgttccgc cgtttgaagg aacttcaccc cggttgcacg attgatgtgt
     3001 tcgcaccgaa gtggtcgatg gcggtgtttg agcgtatgcc ggaagtgaat gagattcttg
     3061 aaaatccgtt cggacacggt gcgttggagt tgaaacgccg ctggcgggtc ggcagggatt
     3121 tggggcggcg cggatacgat caggttatcg tgttgcccgg ttctttgaaa tcggcagtca
     3181 tcgcgctagc aacgggtatt gccaaaagga cgggttatgt cggtgaaagc cgttattttc
     3241 tgttgaacga tatacgcagg ctggataagg aacgtctgcc tttgatggtg gacagatata
     3301 cggctctcgc gcatccgagt caggaagatt ttgacgggca ttcgggattc cccgagtttt
     3361 ccattgatga acggcggcgg gaaatttctg tcgaaacttt cggtttgaat cttggaaagc
     3421 ctgttttggc attttgcccg ggcgcggaat tcgggccggc aaagcgttgg ccgacaaggc
     3481 attttgccga gttgggcaaa cattatctgg cggcgggttg gcaggtttgg ctgttcggtt
     3541 cgcaaaaaga tgatgaaatt gccgaggaaa tcaactgcct ttcagacggt atgtgtgtga
     3601 atttatgcgg aaagaccgat ttgtcgcagg caatggattt gctgtcgttg gcggacacgg
     3661 tcgtgtgcaa cgacagcggg ctgatgcact tggcggcggc attgggcagg aaggtggtgg
     3721 cggtttacgg ttcttccagc ccgacacaca cgccgccttt gagcgataag gcaaaaatcg
     3781 tcagcctcca tttggaatgt tccccgtgtt ttaagcggga atgtccgcta gggcatacag
     3841 actgcctcaa caagctgtat cccgagaaga ttgtgcaggc ggttgaagag gcggtatgat
     3901 gggttctgtt ttcgggttta tgccgtctga cggcggatat tgataggatg tttatggcta
     3961 ttgcgaacaa taaaaaagca tttcacgatt ttttcattga agaccggatt gaagccggtt
     4021 tggtcttgga aggctgggaa gtcaaagcaa tccgcgccgc gcgcgtacag cttaaagaga
     4081 gttatatcta ttggaaaaaa gacgcgtttt atctggtcgg ctgccatatt acggctttgc
     4141 ccacagcctc gacacacatt aaaccagatg ccgtacgtcc gcgcaagctc ttgttgaacc
     4201 agtcggaaat caacaaatta atcggcaaaa ccgaacgtgc cggctacacc atcgtgccgc
     4261 tggatttgca tttttcgcgc ggaaaaatca agatggaaat cggtttggca aaaggtaaga
     4321 aacagcacga caaacgtcag agtatgaaag aggcggattg gaaacgcgag aaacagcggt
     4381 tgattaagca tacgcgctga ttgtggaaac agtgcttcag acggtatggg caaggctgcc
     4441 gcctgatgtg taaacgttct acacctaaac cagaatccgc tttaatcagc acagcatgat
     4501 gccccaaacc ttcatcaaag gtttgagcaa tttgatgata aattggcatt agccaagggt
     4561 aaagtgcggt catttttcct gaaatttttg taaataacga gggcaattat acacattttc
     4621 aaactggctt gacaaaatag caaaaagcaa acgtttacct ttaataaata agaaataaat
     4681 agagaaaaat gtgagctaaa tgacattttt tgttcagttt tatgctaaaa tccacgcccc
     4741 atttattttt acttgacaaa ggaaaattta ttatgaaact cgaagcaagc aagcaagcaa
     4801 gcaagcaagc agaagtttaa aaaatcattt attataagtc tatttttttc tattctttat
     4861 acctctccgc ttttggctgt tgattacgtt tacgacaatt ctaaattaac aaatgagcaa
     4921 attgatcgct taaaaaaact tagagataaa agcagcgaat actggaaaca agaaacttat
     4981 cttctaaaac cgacgaatgg tagctctcag aactctctca aagttcccgc actatactct
     5041 aaaacacacc aattcaaaaa catcaacaac tcaaaaaaga tttcttttta tgacaaggaa
     5101 tacactgaag attaccttgt tggcttcgcc cgaggtttcg gggttgaaaa aagaaatggg
     5161 gaagaagaaa aaacactcca gcaatatttt aaggactgtg taaatactgg gaattccaat
     5221 aatgataagt gtgaacaacc atccttcgaa aactctggcc caagattaat aaagagcgat
     5281 attttcgcct tagctccaca aataaaaaat agccatatca acagcgaaat tttatctgta
     5341 ggtaattata tcgaatggct tcgcccaaca cttaataagc ttcctagttg gcaagagcat
     5401 ctctattctg gtttagatcc attccactat attgaggtta cagacaactc acacgttatc
     5461 ggacaaacaa ttagtttgga tgaattccgc ttagaaaatt ctctctggga accccgttgg
     5521 gattctgatg ttggggaatt aaaaacaacg aatgcagaca tccgttttaa tactaaaagt
     5581 gaaagtttac tcgtaaaaga agattatgct ggcggagctc gttttcgttt tgcttacgat
     5641 ctaaaagata aagtctctga aacaccagtt ttaacttttg aaaaaaatat aactggcact
     5701 tccgacatca tcttcgaaaa tccaatagac gatttaaaaa gtctcgacgg gcatcaaatt
     5761 atcaaagtaa atggcacagc agataaacac gcattccgtc tttctggcaa acaccaaaag
     5821 ggaatttata cgctttcttt acaacaacgc ccagaaggct tttttaccaa agtgcaagaa
     5881 cgcgatgata tttcgattta tgcacaacag gctcaagccg ccaatacctt attcgccttg
     5941 cgtttgaacg acaaaaacag cgatattttt gaccgcactt taccacgcaa aggcttgtgg
     6001 ttgcgtgtga ttgacggaca ttccaaccaa tgggtgcaag gcaaaacagc acctgtagaa
     6061 ggctatcgta aaggtgtgca actcggtggc gaagtattca catggcaaaa cgaaagtaat
     6121 caactttccg ttggcttaat gagcggacaa gcagaacaac gcagcacttt ccgcaatcca
     6181 gatacagaca atcttacaac gggaaatgtg aaaggctttg gggcgggcat ttatgccact
     6241 tggcatcagc ttcaggacaa acagacaggt gcgtatgcgg atagctgggt acaatatcaa
     6301 cgcttccgcc accgcattaa cactgaagat gctacagaac gttttacttc aaaaggtatt
     6361 accgcctcaa ttgaagcggg ttacaatgct ttattggcag aacacttcac taaaaagggc
     6421 aacagccttc gtgtttacct acaaccacag gcacaattga cttatttggg ggtaaacgga
     6481 aaattcagcg atagcgaaaa tgcccacgtg aatttacttg gctcccgcca attacaaact
     6541 cgggttggcg ttcaagctaa agctcaattt tcactctata aaaatatcgc tattgaacct
     6601 ttcgccgctg tgaacgcgct ttaccacaac aaacctttcg gtgtggaaat agacggagag
     6661 cgtcgagtga taaacaataa gacggcaatt gagagccaat taggtgtagc agtgaaaatc
     6721 aaatctcact taactttaca agcctcattc aaccgccaaa caagcaaata tcatcaggct
     6781 aaacaaggtg cgttgaattt gcagtggacg ttttaagtta agttgtaaca aaataaaatc
     6841 ggcagggaaa ttcactgccg atttttttat tttaaaaatg cggtcatttt tcgtgtcatt
     6901 tccaccaatt ttttaccgca ctcacgagct cgcgctaaac cgacaatcgg atctattgtc
     6961 cctttctttg ttgtgtatgc aaatcagaaa tgccggttac ggaagcagtt tgcccggatt
     7021 cataatgtta tatggatcaa ggtgttgttt gatgcttttc atcagggcga tttcggcagg
     7081 cgtgcgtact ttgtccagcc actgtttttt gatgatacct atgccgtgtt cggcggcaat
     7141 cgtgccgttg caggcaagaa cgttgcgata aactgcgtcg ttgatgtcgt tttcgtaacg
     7201 atagacttca ttgctgagga tttcgggcag gaaagtattg tagtgcaggc tgccgtcgcc
     7261 aagatgtccg aagcagacga tttgtatgcc tttaaaattc tgttccaaat ctttggcgca
     7321 ccggcggaca aagtcggcaa cgcgtccgat aggaatggca atatcgtgtt tgatgctggt
     7381 gcccagtttg cgttgcgatg cggagatgtt ttcgcgcaat gcccacatat ggatacgttc
     7441 ttgttcgctt tgtgccaata cgctgtcggt aaatcctttt ttataaagaa attcgacaag
     7501 ccgatcatca agattgctgt cgggtaatga gtcggtcaac tcaagtaaaa tatgccattc
     7561 tgaatgtgtc ggcaggggga atttgctgaa ttcggaagac aactcggcgg caaaacggcc
     7621 gaccagttca aaactgcata ggcgttcggc aaagtgagct tgggtttcgg tcagcaggcg
     7681 gacggcggat tcgatgtcgg gtatgccgac ccatgccgtt gccttgtccg agggacgggc
     7741 aaaaagcttg agtgtggcgg cagtgatgat gcccagtgtg ccttcgctgc cgataaacag
     7801 atggcgcagg tcgtagccgg tggtgttttt atgcagggga tggagatggg aaaccagctc
     7861 gccgttgggg aggacgactt ccaaaccgat aatcaggtcg cgcatcgtgc cgtaacgcaa
     7921 tacgttcaaa cctccggcat tgcaggcgat gttgccgccg atttgacagg agccttcgct
     7981 ggcgagactg agtgggaaca gcctgtttga ggcttcggct gcctgttgga cggtttggag
     8041 tacggaacct gcttcgacgg ttatgcagtt gtctgacaaa ttgatgctgc ggatgcggtt
     8101 gagtttggaa aggttcagca atacgccgtt ttccgatact gccgcgccgc acaaaccggt
     8161 attgccgcct tgcggcgtaa ccggaatacg gtgttcgtaa caaaaacgca taatggtttg
     8221 cacgctttca acgctgcgcg gctgcaaaat gatgtctggt gcagacgtaa agcggttgcg
     8281 ctggtctttt aaaagcgtcg gagaaagttc ggcaatttca tctgccggca ggagtcggga
     8341 aaattcggta tgaaggtcag gcatgtttgt cggaagggta gggagaagat tgaggaaggg
     8401 aatctgatgc cgtctgaaaa atcaaaaaag caggatactg aacgtatcct gcttttttgc
     8461 aagcggaaaa tgcttatttt gcagcttcgg cagcaggagc ttcagcggca ggtgcttcgg
     8521 tagcggcagc ttcagcagcg ggagcttcgg cggcaggtgc ttcagtagcg gcagcttcag
     8581 cagcgggagc ttcggcggca ggtgcttcgg tagcgggagc ttcggcggca ggtgcttcag
     8641 tagcgggagc ttcagcggca ggtgcttcgg tagcgggagc ttcagcggca ggtgcttcgg
     8701 tagcgggagc ttcagcggct ttttcaccgc cgcaggctgc caggaccaaa gacaacaaag
     8761 cagcggcaaa cagggatttt ttcattttag taacctttag aaccaattta aattattgaa
     8821 acaaataggt cttttatcga ccatactaca aaccgggttg gaaattcatc gctgaatttg
     8881 cgtaattatg cactatggaa taagggattg caatgtcgta cagatgggaa aaaggtttga
     8941 atgaaaagtt ttctggcggg cggattgata aatcctgtaa aacggttttg ctgttaaaaa
     9001 ttcgataggg ataaatatta tagagattaa tataatttca gtatcttgta tgatttatta
     9061 aggcggattg ggaaagtcgg cagacaggtc ggaatggttt taagaatgtt gtaaaaaagt
     9121 tacaccgcaa taaaataaca aaaatttaca ttgaaatatg tgctgtacag tttatgaaca
     9181 ggttaacggt aatcggccgg cgcggcggtt gtgttagaat gcgcctgtaa tcaatctgct
     9241 gttcctgaaa ggacaaatat ggctatcgtg aaaaattccg tggtttcgct gcattatgag
     9301 atgtatgatg ccaacaatca gcttttggac aaaaccgaag aaccgattgc gtatctgcac
     9361 ggcggttacg acggcatttt ccctttggtg gaagaggcgt tgcacggtaa ggatgccggc
     9421 gatacggtcg atgtggcgct gtcgcccgac gatgcgttcg gcgagcagga tccggagttg
     9481 gtccgtattg aagatgcggg cgtgttccct gttgaagtcg aagtcggcat gatgtttgaa
     9541 gccgacgatc ctgaaaccgg cgatgttgtc gtctatcgtg taaccgatgt tgccgacggc
     9601 aaggcggtgg tggacggcaa ccatcctttg gcaggcatga aaatccgctt taaggctacg
     9661 gttgaaagtg tgcgcgatgc atccgatgag gaaatcgcac acggtcatgt ccacggtccg
     9721 cacggtcatc accaccactg attgccggca gcttggaggt atctgatgcc gtctgaaaag
     9781 ggtttgttcc atttcagacg gcattttgtt tgatggatgg agaaggtatg tcggttgggc
     9841 attatgaaaa ttttcccgtc ggttcgctga ttttgccgcg caggttgagg aagccggttc
     9901 acgcggtgta cgcgtttgca cggacggcgg acgatatggc ggacgagggc agtatgccgt
     9961 ctgaagccag gttggcgggg ttggagggtt tgcggcgcga gttggacgtg ttggcatcgg
    10021 gcggccggtc ggcgcatcct ttgattgcac gattggatgc cgaggcggtt gtgccgttcg
    10081 gtttggattt gcagccgttt tatgatttgc tttctgcatt ttctcaggat gtggttaaaa
    10141 caaggtatgg aaatttcggc gagctggccg attattgccg acgttctgcc aatcctgtcg
    10201 gacgcattat gctggctttg tacgggaaaa cggatgcggt gtgcgtggcg caaagcgacg
    10261 gcatctgtac ggctttgcaa ctggtgaatt tttggcagga tgtggctgtg gattggcaaa
    10321 agggcagggt ttatattccg caggacgatt tgttgaaatt cggtgtttcc gaaggacaga
    10381 ttgcggaggg tagggcggat gcggcgtttc agcggctgat ggcgtatgag tgccggcgcg
    10441 catttcgtat gctgaaggcg ggttcgcctt tagcgcgcga attgaaaggg cgtatcggtt
    10501 tggaactccg tatgattgtg ttaggggcgc agttgatttt gcagaaactg gacgcgtgcc
    10561 gatacgatgt gtttgcacaa cgccccgttt tggataagaa ggattggttg attatgctga
    10621 aacgcgcgtt gtggaaatga tgggggaaat gcatgccgtc tgaaaggggt tgttccgctt
    10681 cagacggcat tttgcaggcg gttggtatgg tcaggcgatt tcatgtgggt tcaacctggc
    10741 ttgatcttct gcacccaagc cgatgagttt gcgcagtcgg gtcatgtaga aattgacatc
    10801 caaaccggtg ccgtagcgtt gcgccgtcca cagtgtttcc gccagtgcct ccatcatttc
    10861 gtgttcggct tccagccagc cgcgtttggc gcacagggtg tcgtggattg cgcgtatgcc
    10921 gtgcggctgg tctatgcccg cctgttcttg gacggacaga tgcagcgaca tatgcaggaa
    10981 ggggttgctt tcgccgtttt cgggcagcca gtcggtgtcc agatggtctt cgatgcgttc
    11041 gagatagcgg tggtattcgg gatgggcttc gacaatgcgg agggctttct gttccagtgc
    11101 gctcagttgc agcggattga gccgctgctg ccacacgcgg gcgaaaaagc ggcggacatc
    11161 gtgggtattg acatcgtaca tggcgggttc gataagatgg ttatggaaat aggttcggat
    11221 aatggcgggt tttacataaa ttaaatccca ccgtcaacct taccatttat aatgcagaca
    11281 aattatttgt ctgaaacgga ttcgattatg aaaaaactga tttgcctgtt cgccgtattt
    11341 ttgatgttgt gcggacgagc tttcgcgctg gatgcgaacg atctgctgcc gccggaaaag
    11401 gcattcgtgc cggagcttgc cgttgccgac gacggtgtga acgtccgttt caagattgcc
    11461 gacggatact atatgtatca ggcgaaaatc gtcggcaaga ccgatccggc ggatttgttg
    11521 ggacagcctt ctttcagcaa gggcgaagag aaggaagacg agtttttcgg caggcagacg
    11581 gtttaccatc acgaagcgca ggttgccttt ccttatgcaa aggctgtcgg cgaaccgtat
    11641 aaattggttt tgacctatca gggctgtgcc gaagccggcg tgtgctatcc gcccgtggat
    11701 accgagtttg atatttccgg caacggcact taccatccgc aaaccgacga accggcatcc
    11761 gccaaagacc gctttttgca gccttcctct caaaacggca gcggggcgct gccgcccccg
    11821 aagggggatg agggcggcga cagccgtttc aagctgtctt gggatacgct caacgccaat
    11881 cttttggcgt tttttctcgc cggtttgggt ctgagtttta ccgcctgtat gtatcccctg
    11941 ttgccgattg tttccagtat tgtggtcggc gacaaaaagg cgggcaaggc gcgggcgttt
    12001 gtgctgtccg tcgtttatgt tcagggtttg gctctgactt atacgctggt cggcattgtt
    12061 gccggactga cgggcgcact gctgaccgta tggttgcagc aggcttgggt cgtgttggcg
    12121 gcatcggctt tgatggtcgt cttggcactg tctatgttcg ggctgttcaa catccagctt
    12181 cccaacgccg tgcagtcgta ttttcagaat caaagcagca ggctttcagg cggtaaaatc
    12241 gtttccgtct ttattatggg catattgtcc gcgctgattg tcgggccgtg cgtcgccccg
    12301 ccgctggcat ttgctttggg ctacatcggt cagacgggcg atgcggtttt aggcggtttg
    12361 gcactttaca ctttggcgtt gggcaccggc gttccgctga ttgccatcgg cacgttcggc
    12421 gggcatatcc tgcctaaggc aggcgattgg atgaatgccg tcaaatacgc tttcggcttt
    12481 atcctgcttg ccgtcgccgt ttacctcgcc acgccgcact tgccctatta tctcgtcgtc
    12541 gcgctgtaca cgctgctgat gctggttcct gcctgtatgc tgctggtcaa cggacgcagg
    12601 cagaaacgcc gtccgaaagc tgtggcattc gcattgggca gcatattgct gataggcggc
    12661 gcgtggttcg gctggcaggg cgcaaacggc aaaacgaccg cgctgcacca tttcctgacc
    12721 ctcaatccgc cggccgaagc aggcaaatct tcggaacacg gcaaaatgtt tgccgatact
    12781 gccgcgctga aggcagcgat ggatacggcg ttgaaagaac atcccgacaa acccgtcgtt
    12841 ttggattttt atgccgactg gtgcatttcc tgcaaagaaa tggcggctta cacgctcaat
    12901 cagccggaag tgcatcaggc agtcgatatg gaacgctttt tccaaatcga cgtaaccgcc
    12961 aacacgcccg aacatcaggc gttgttgaaa gaatacggtc tgttcgggcc gccgggcgtg
    13021 tttgtcgtcc gctccgacgg cagccgcagc gagccgctgc tgggttttgt caaagcagac
    13081 aagtttatcg agtggtatga acaaaaccgc tgattccgat gtttcaaatg ccgtctgaag
    13141 ttctcagacg gcatttttgt cagaaaggaa aacggcaata tccgctatac acacggaagc
    13201 agtccaaaga tgtgaaaacc gtgtgtataa gttagtttgc gtattgaaat taaaagataa
    13261 aaatacgatg attaaaaaat aggcataaga aaagataaac acagccggtg tttgccggtg
    13321 tgtgtatctt ttgtggaaaa acatcttaat gtcatgtatt ttatggaaaa taataacatg
    13381 attaaaaaat agtcaatcat taaggttcag gtgggcggct gcaaccgtca atgtctgaaa
    13441 agaggcagtg ctgtttaagt atggaaagtc ggatatagtg gattaacaaa aatcaggaca
    13501 aggcgacgaa gccgcagaca gtaca
//
LOCUS       DAXHMX010000045        13485 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHMX010000045 DAXHMX010000000
VERSION     DAXHMX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.045"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(144..234)
                     /locus_tag="ACFM4S_000942"
     tRNA            complement(144..234)
                     /locus_tag="ACFM4S_000942"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(273..359)
                     /locus_tag="ACFM4S_000943"
     tRNA            complement(273..359)
                     /locus_tag="ACFM4S_000943"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:cag)
     gene            complement(395..481)
                     /locus_tag="ACFM4S_000944"
     tRNA            complement(395..481)
                     /locus_tag="ACFM4S_000944"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Leu,seq:cag)
     gene            570..2945
                     /gene="rnr"
                     /locus_tag="ACFM4S_000945"
     CDS             570..2945
                     /gene="rnr"
                     /locus_tag="ACFM4S_000945"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6050653.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIEALVRELSIKEEEYEFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="ACFM4S_000946"
     CDS             complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="ACFM4S_000946"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6050654.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4948..5028)
                     /locus_tag="ACFM4S_000947"
                     /pseudo
     CDS             complement(4948..5028)
                     /locus_tag="ACFM4S_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5074..5535)
                     /locus_tag="ACFM4S_000948"
     CDS             complement(5074..5535)
                     /locus_tag="ACFM4S_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050655.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(5626..8184)
                     /gene="glnD"
                     /locus_tag="ACFM4S_000949"
     CDS             complement(5626..8184)
                     /gene="glnD"
                     /locus_tag="ACFM4S_000949"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6050656.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(8232..8552)
                     /locus_tag="ACFM4S_000950"
     CDS             complement(8232..8552)
                     /locus_tag="ACFM4S_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6050657.1"
                     /translation="MMGNKLTPPAKLPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(8877..9350)
                     /gene="bfr"
                     /locus_tag="ACFM4S_000951"
     CDS             complement(8877..9350)
                     /gene="bfr"
                     /locus_tag="ACFM4S_000951"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6050658.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(9378..9842)
                     /gene="bfr"
                     /locus_tag="ACFM4S_000952"
     CDS             complement(9378..9842)
                     /gene="bfr"
                     /locus_tag="ACFM4S_000952"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6050659.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(10305..11288)
                     /gene="lipA"
                     /locus_tag="ACFM4S_000953"
     CDS             complement(10305..11288)
                     /gene="lipA"
                     /locus_tag="ACFM4S_000953"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6050660.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQATEALRESHGGCGHH"
     gene            complement(11281..11904)
                     /gene="lipB"
                     /locus_tag="ACFM4S_000954"
     CDS             complement(11281..11904)
                     /gene="lipB"
                     /locus_tag="ACFM4S_000954"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6050661.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLVGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11906..12181)
                     /locus_tag="ACFM4S_000955"
     CDS             complement(11906..12181)
                     /locus_tag="ACFM4S_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6050662.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            12366..13436
                     /locus_tag="ACFM4S_000956"
     CDS             12366..13436
                     /locus_tag="ACFM4S_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6050663.1"
                     /translation="MYRRKGWGIKPWMGAGAAFAALVWLIFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIS
                     FMQNTLLPWLKNTIGGYMEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
BASE COUNT         2941 a         3179 c         3816 g         3549 t
ORIGIN      
        1 ataccacaaa acataccata aaaaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggga taatttatta tttggtgccc gggagaagac tcgaacttcc
      301 acacccgtga ggataccagc acctgaagct ggcgcgtcta ccaattccgc cacccgggca
      361 atctaaatat tgaaataaag caaagcattt gatttggtgc ccgggagaag actcgaactt
      421 ccacacccgt gaggatacta gcacctgaag ctagcgcgtc taccaattcc gccacccggg
      481 ctttgcttta tttgctgttt tgcggtacag tgtcctgccg caaagaagtc gctattatat
      541 agttcataaa gagaatgtca acagtccaaa tgaataaaaa tattaaatct ttaaatttac
      601 gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta tgaacatcct ttgcccagtc
      661 gggaatggat aatcgagctg cttgaacgta aaggcgtacc atccaagatt gaagctttgg
      721 tacgcgaatt gtcgattaag gaagaagagt acgaattttt cgaacgtcgt ctgaaggcga
      781 tggcgcggga cggtcaggtt ttaatcaacc gtcggggcgc ggtttgcgcg gcggacaaat
      841 tggatttggt caaatgccgt gtcgaggcgc acaaagacgg cttcggtttc gccgtgccgc
      901 tcacgcccgc caaagacggt gattttgttt tgtacgaacg ccagatgcgc ggcattatgc
      961 acggcgatat tgtcactgtt cgtcctgccg gcatggaccg taggggccgc cgcgaaggga
     1021 cggttctgga tattgtcgaa cgcgcgcaaa gcaaagttgt cggccgtttc tatatggata
     1081 ggggcgtggc gattttggag ccggaagaca agcgtctgaa ccaaagcatc gtattggaac
     1141 cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt catcgtcggc gaaattgagg
     1201 tttatcctga gcaaaaccgg ccggcagtgg caaaaatcat cgaagttttg ggcgattatg
     1261 ccgacagcgg catggagatt gaaattgccg tgcgcaagca tcatttgccg caccaattca
     1321 gtgaagcgtg tgccaaagcc gcgaaaaaaa ttcccgacca tgtacgcaaa agcgatttga
     1381 aaggccgcgt cgatttgcgc gatttgcctt tggtaacgat agacggcgaa acggcgcgcg
     1441 attttgacga tgccgtgttt gccgaaaaaa tcggacgcaa ttaccgtctg gtcgtggcga
     1501 ttgccgatgt cagccattat gtccgccccg atgacgctat cgacacggac gctcaggaac
     1561 gcagcaccag tgtttacttc ccgcgccgcg tgattcccat gttgccggaa aacctgtcca
     1621 acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg tatggtgtgc gatatggtca
     1681 tcacttacgc gggcaatatt aaagaatacc gcttctaccc cgccgtgatg cgctctcatg
     1741 cccgcctgac ctacaaccaa gtttggaaat ggctttcaga cggcatcggg catccgttca
     1801 aagcacaaat cgacacgctt tacaaactct tcaaaatcct tcagaaaaag cgtttcgaac
     1861 gcggggcggt ggagtttgac agcatcgaaa cccaaatgct tttcgacgac aacggtaaaa
     1921 ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa gctgattgaa gaatgtatgt
     1981 tggcggcaaa cgtttgcgca gcggattttc tgttgaaaaa caagcatacc gcattgttcc
     2041 gcaaccattt ggggcccacg cccgaaaaac tcgccgcctt gcgcgagcag ctcggtctgt
     2101 tggggcttca acttggcggc ggcgacaacc cgtcgccgaa agactatgcc gcgcttgccg
     2161 gacagttcaa aggcaggccg gatgccgaat tgctgcaagt catgatgttg cgctccatgc
     2221 aacaggcggt ttacgaaccg cattgcgacg gacactttgg tcttgcctac gaagcatacg
     2281 cccacttcac ctcgcccatc cgccgctatc ccgacctgac cgtacaccgc gccatcaaag
     2341 ccgtgttgaa tcagcaaacc tacacgccaa aaaaaagctg gcaggctttg ggcgtgcata
     2401 cctcgttctg tgagcgccgt gccgacgacg ccagccgcga cgtggaaaac tggctgaaaa
     2461 cctattatat gcgcgataag gtcggcgaag tattcgaagg taaaatctcc ggcatgacca
     2521 gttttggtat ctttgtaaca ctggacggca tccacattga cggcttggtg catatcagcg
     2581 atttgggcga agactatttc aacttccgcc ccgaaatcat ggcaatcgaa ggcgaacgca
     2641 gcggcatccg tttcaacatg ggggacaggg ttgccgtccg ggtcgcccgt gccgatttgg
     2701 atgatggaaa aatcgatttt gtcctgattg ccgaggggag cggcaggggg cggaaagtta
     2761 aatcatccgc gtctgccaaa ccggcaggga cggcggggaa agggaaaccg aaaaccgccg
     2821 ccgagaaaaa aacagcccga ggcggcaaag taaggggaag gggcgcgtct gccgccgcag
     2881 aatcggggaa aaaggcaaag aaaccggttc cgattaaggt aaaaaaacgg aaaggcaaat
     2941 cataatgctg acggggtggc ttggggaggg cggggcatac tttcccgaag ccgccgcccg
     3001 attttttgat atggcggccg gcgcatcatt tcaggccaat cgaaacgccc gtattgaaag
     3061 attgcgttta tttccaccgc cgttttaaag gccggcggta ttcggcagac ggggcgcaaa
     3121 cggcgttcag acggcatttt cattctttcg gcgtgtccgt ccgaatggct ttgcccgtcc
     3181 gcgcaatcag cccgtgcggc ttgccttgaa cggacaaaaa atgccgtctg aaacccgaaa
     3241 atcaggtttc agacggcatt ttgccttgaa aaggctgttc aaatcagcgg tggtagttcg
     3301 gtgcttcttt agtgatttga acgtcgtgaa cgtgcgattc gctcatacct gcggaagtga
     3361 tttccacaaa ttcggctttt tcgtgcattt cggcaatatt ggcgcaaccc aagtaaccca
     3421 tactggaacg cagaccgccg gtcagctggt ggatgatgtt cacaatcggg cctttgtaag
     3481 gaacgcggcc ttcgatgcct tcggggacgt atttgtcggt gctgtcggtt ttgtcttgga
     3541 agtagcggtc ggcagaacct tggctcatcg cgcccaatga acccataccg cgataggatt
     3601 tgtacgagcg gccttgatag agttcgattt cgcccggcgc ttcttccgtg ccggcaaaca
     3661 taccgccgag catgacgctg tacgcgcctg cggcgagggc tttggcgatg tcgccggaga
     3721 agcggatgcc gccgtcggca atcaggggaa cgcctgtgcc tttgagggct tcggcaacgt
     3781 tgtggatggc ggtcagttgc ggcacgccca cgcctgccac gatacgggtg gtgcaaatcg
     3841 atcccgggcc gataccgact ttgacggcat ccgcaccggc ggcgaccaaa tccaatgcgg
     3901 ctttggcagt ggcgatgttg ccgccgatga cttggatgtg cggataggtt tctttcaccc
     3961 atttcacgcg gtcaatcacg ccttggctgt gcccgtgggc agtatcgacg acaatcacgt
     4021 ccacgccggc ttcgaccaag gctttgacgc gctcttcggt atcgccgccg gtgccgactg
     4081 ccgcaccgac gcgcagacgg ccttcggagt ctttgttggc gttgggaaac tcggtggttt
     4141 ttaaaatatc tttaacggta atcagacctt tgagttcgtc tttttcgttc agaaccaaaa
     4201 cgcgctcgac tttgtgcgtg tgcatcagtt cgcgcgcttc gtctatgctt gtgccttcgg
     4261 ggacggtaac caaacgttcg cgcggggtca taatggcgga aacgggcaaa tcgacgcggt
     4321 tttcaaaacg caggtcgcgg ttggttacga tgccgacgac tttgccgttt tcaacgacgg
     4381 gcaggccgga cattttgcgt ttgcgctgcg cacgcatttc caagacttcg cggatgagcg
     4441 tcgtcggtgc aacggttacg gggtctttga ccacgccgct ttcgtggcgt ttcactttgg
     4501 aaacggcgcg cgcctgcatt tcgggcggca tgtttttatg gatgatgccg atgccgcctt
     4561 cctgtgccat cgaaatggcg aggcgcgcct cggtaacagt gtccatcgca gcggaaagca
     4621 gggggaggtt gagtgtgatt tcgcgggtga gcttggtttg aagtttaacg tctcgcggca
     4681 gcacggtcga atgtgcggga accaacaaaa catcgtcgaa agtataggct ttttctacga
     4741 tacgcataat gctcggtctt tcagtttgtg caagatgcac ggcattatag cacgttaccg
     4801 gcggcttgac agtttatcag gtttaatttt ggtccccttt gaatagcccg gttttccttt
     4861 gccgaccact gttgctcccg ttctttcaat ttcaggaaaa gcttttttct aatttttggt
     4921 aagtggctca gttattgaag ccctatatcg ggcggtaact tccagcacaa aaaaacggag
     4981 tagttcttta tttatttttt cctttaattt tcagtatatt atcttaatat ttcgagggta
     5041 acatatctgc taatctagtt acagccccat atattataga ttcaattgaa aaataacaga
     5101 ttcaactgta cctttcttat acctgatttc tttaaagttt ttcccattgt caaaactata
     5161 aaaaagtctc tcttcctcac cagaatatcc catttctaat actaaacaat ctatttcaca
     5221 attaccccct ccactcatat atgtagtatc aatagaaaat atattgcata attccagaga
     5281 attatattta tctatatcaa atagttgctc tccaaaatct aaaccattgt ttccacctgc
     5341 ataatacttc tcttcaaagg atgacttcaa actattaaaa actgcattta gcgtaaaata
     5401 tccatatggg catatcttag gatatatatt ctctcctatt attatctgaa tttttccata
     5461 aataaaacca ttacattcag agttttcttc ccataaaatc ccaaattttc ttgttgagtc
     5521 cataataata ttcatataaa tccttatatt aataaattat ttacaatatc ccccgctttc
     5581 agacggcata cggcgtggcg gcggaatgcc gtctgaaggc gggcgttatg ataattgttc
     5641 cagcagcgtc tgcttcaatg aggactggat ttttgggttt ttcaggtcta ggctaaaaac
     5701 ggtaaaactg tcttcggcgc gttcatccag tgtggagatt ttggcatagc gcaggctgac
     5761 gttgtgggcg aaaaagactt ccgccatatc ggcgagcagg aaggggcggt tgacggcggt
     5821 gatttcgacg gagtaccaat cggggtagtc ttcttcgggg gtgatggtga tgcttggcgc
     5881 gatcggcata tagcggctgc ggcggctgat gcggcggcta tggctggaga tttcggcaac
     5941 ggtgtgtccg tggataaagc tgttgagttc ggcttcgagc gcgctttgaa tgtcggggta
     6001 gtcttcggga gcgtgctgcg aggggatttg cacgatgaag gtgtcgagga tgtagtcgtg
     6061 ttcggtgatg aaggcgcggg cggcgagaat gtcgaagccg tggcggctga agatgcggca
     6121 gaggcgggcg aacaggcgcg ggccgttggg cataaagacc atcacttgga agctgtcgct
     6181 ttggggcagg atgcggctgc ggacgatggg ggtttcaaaa tcgtgtacga ggttggcggc
     6241 gtgccacagg atttcgcggg actggtggcg ggcgaagtag gcggaaccga gcgcgttcca
     6301 tagttttttc tgctgttttt cggggacggc ggcgcgggtg agtaagtcgg cggcttcctg
     6361 ccggcggcgg ccgaagaggg tgtgcgggtt gccgccgttg cctgtaaggt agcgtccggc
     6421 ggcatggaag aggctttcca gcaggctggc gcgccatgcg ttccacagct tgggattggt
     6481 accgcgtatg tcggaaatgg tcagaaggta gagcgcgctg aggcgttcgt gggtttggac
     6541 gcgtttgcag aaagcatcga gtacgtcggg gtcttggatg tcttcttttt gggcgacggc
     6601 agacatcagc aggtggtttt cgaccagcca agcgagcagg tcgctttctt ctccggtcag
     6661 gaagtggtca gcggcaaatt ggcgcgcgtc tgcgatgcct tgtatggcat ggtcgccgcc
     6721 gcgtcctttg gcgatgtcat ggaaaaaggc ggcaaggtag aggatgtctt gtttttcaaa
     6781 ggactgcatc agtgcagagg cgtagggcag ctcgtggctg tgcatatcca aggcaaggcg
     6841 gcggacgttg cggacgacgg tgaggatgtg gtcgtccacg ggatagatgt ggaacaggtc
     6901 gtgttggagc aggccgatga ttttttccca cgcgggcagg tagcggccca acacgccgta
     6961 gaggttgaga aagcgcaggg tctgggtcag cccgttgccg ttgcggaaaa aaccggcgaa
     7021 gcggcggcgg ttttcgggat tttggtagaa gctgcggttg attttgcgcg tcgcgcccca
     7081 ccaggcgcgc agggtttgcg gttcgagcgc ggtaatgtcg ttgcgctgct gcatgatttc
     7141 gacgattttg aaaatgtgtt cgggccgtct gaaaaaaata tcggtgtgcc gcgcggcgat
     7201 ttggttgttg acttggatgt agtcgtcgtc aatccgcagg gtaacgcgca acggggtgga
     7261 ggaaacgcgg ctttgcagca taggcgtgag gatgccgccc agttgtttga cggttttaat
     7321 cgcgcggtaa aacacgcgca tcagttcttc gctttggcgg cggaggttca agccttcata
     7381 acccatgctt tcggcgactt gcggctgcaa atcgaacagc aggcggtctt cggcgcgatt
     7441 ggcgtttaaa tgcagatgga tgcggatgtg ggcgaggcgg cggtagccgt gcgaaagcat
     7501 accggcttcg gcacgcgtca aaatccgctg tttgagcagg tcgggcaggt cggtcgccaa
     7561 gccttgcgcc ttcgctatcc aaagcagggt gtggatgtcg cgcagaccgc ccggacagct
     7621 tttgatattc ggctccaata ctgcccccga accttgcgat ttggcgtggc ggtgttccat
     7681 ctccaccagt tttgcctcga caaacgccgc cacattgcgc tgcgcgttca ttttttccgc
     7741 cagtttatcc accgtttggc ggttgccaaa caaaaaccta gcctctaaaa acgctgtgtc
     7801 ccccgtaata tcattgcgca cgctttcaca tagttcatca acgctgccgc tttttacaga
     7861 cggcatcagt ttgcagtccc acagggtttg aacaaaccgg gcaatctgtt cctgaatgcc
     7921 gtctgaaagc ggggcagggg agacaaccgc caaatccaca tccgaacagg gatacagttc
     7981 gccgcgcccg aagccgccca ccgccatcag gcataacgcg ctgttttgaa aatgttctac
     8041 ccacaatgcc gccagtaagg tttcgactgc cgccgtatat tccctgaaaa ataccgacac
     8101 gcggttggct ttcaaataat gcgcttcggc ggcatcgcgc tgccgtttga aggtttccag
     8161 tgctgaagac aggtttgcag gcatttttat tctttcgatt ggcgggaaaa gggaggtgga
     8221 tggttcggcg gtcaaatacc gctttcagac ggcatttgtc gggtatcggc ggaattggtc
     8281 atcagcttca accgttcctg cggcggcagc agcaactgat acgccgccac gcccaaagcc
     8341 gccgccattt tttcgatatt cgacagggcg atgttccagc gtttgcgctc gactgccgac
     8401 acataagtcc tgtccaaacc gcattgccgc gccaattctt cttgcgacca acccttgttc
     8461 acgcggaaaa gccgcatatt gtatgccaat accgcccgca aatcctgttc gtcaggcagt
     8521 ttggcaggcg gggtcaattt gttgcccatc atttgcttcc agataaatgg ttaaagttaa
     8581 gcatttgctg ttacggattt tacttcacat aaaagccaaa aaaaaggggt ggcggcagcc
     8641 acacccaaac acacacacat caagaggaaa gaggtaaaat caagacagat tgggaaacag
     8701 caaacaagca atctagattt catatcggta ttatcaaaca aaaaccgtta tcgagtattg
     8761 attcaaatca atttagattt gattcgcaaa ccggcaaaga aaaacggcat acccgtttat
     8821 acggatatgc cgtctgatga tgcaggggac tgctatattt ggcagtggtt tgtgttttag
     8881 tcctcttgcg ccgctgtttg caggtagttc ggcaaaccaa ttttgccgat cagttcctgc
     8941 tgggtttcaa gccagtcgat gtgttcttcg ttggtgtctt tttgtttttc caacaaatca
     9001 cggctgacgt aatcctgttg cgcttctgct gtggcgatgg cggcaagcag ggcttcgtgt
     9061 ttttcctgtt ctttggtcaa atcgcaggcg atgatttctt cggtggactc gccaatcaga
     9121 agcttgccca gttcttgcag gttcggcaga ccttcgagga acagaatgcg ctcgatcaaa
     9181 tcgtcggcag ctttcatttc aacgatggac tgtttgaaga aatgttcgcc cagttcttca
     9241 aagccccagt ttttcaaaat acgggcgtga aggaaatatt ggttgatggt taccagcagc
     9301 aagcctaagt ttttgtttag ctcgcggata accagacggt cgcctttcat acggactcct
     9361 tttattccga atacggatta cagttggctt tggtagtagt tgccttcgcc aattaactcg
     9421 atcaggcgaa gctgctgttc cagccagtgg gcgtggtctt cttcggtatc tttcagttgg
     9481 gcaaccatca ggtcgcgcgt aacatagtct tgagcctctt cgcacagttt gatgcctttt
     9541 ttcaacgcat cacgtacttc atattcggtt tgcaggtcgg ctttgaggca ggaaaccacg
     9601 tccgtgccga tattcagttc ggcgcgtgcc attttcggcg tgccgcccag catcaggatg
     9661 cggcggatga agtcttcggc gtgtgtggtt tcttcttcca tctcgtggtt gagacgttca
     9721 aaaagtttgg tgtagcccca ttcggagtag aggcgggagt ggataaagta ttggtcgcgt
     9781 gccgccagct cgccagacag caattcgttc atgtaatcta aaactgcttg attgccttgc
     9841 ataatatttc tcttttctta atttgggttt cgtgtagtta aacagtggat gtagcactgt
     9901 tcggaatgcg tgcattgtat gcaaaagctg tcggcatgac aaattttcta tttaaaatac
     9961 aaacaattat caaaagaaat agggcgtatt ccccgatgcc ttccaaatca gtatgctgtt
    10021 ttttatcggt tttttgtgct gttaaaacta ataatccata ttatccataa agaacattta
    10081 cacttgttaa ccatagctaa aagcaaatac ggtacggttt tttatcaaaa tttataatga
    10141 atcgttctca ttaaccgaca gattcttttg agattatcgg attttgggaa taaattatgc
    10201 cgtctgaggg gctttcagac ggcatagaca attaaagctg attaaacaac gcggcagaca
    10261 gcagcgtagg aaattatttt ttacttcgga acggtatttt gggtttaatg atgcccgcaa
    10321 ccgccgtggc tttcgcgtaa ggcttcggta gcctgttcgt cggcgtggta gctggaacgt
    10381 accatcgcgc cgatggcggc attgctgaag cccagttcgt atgcttcttt ttcaaagatt
    10441 ttgaactgct cgggcgtaac gtagcgcagg acgggcaggt gtccgtctga aggctggagg
    10501 tactgtccga tggtaatcat ttcgatattg tgcgcccgca tatcgcgcat aatttcacgc
    10561 acgtcttcgt ctgtttcgcc caagccgacc atgatgccgg atttggtcgg gatgtgcggc
    10621 atcatttctt tataacgttt taataagtct aaagaatgtt gataattggc accgggacgg
    10681 gcttttctgt acaggctcgg atgggtttct aggttgtggt tcatcacgtc gggcggggtt
    10741 tcggcaagga ttttgagtgc gatgtccaag cgtcctcgga agtcggggac gaggatttcg
    10801 attttggtgt tcgggctggt ttcgcggatg gctttgatgc agtcggcgaa atgctgtgcg
    10861 ccgccgtcgc gcaggtcgtc gcggtcgacg gaggtgatga cgacgtaacg caggttcatg
    10921 gctttgacgg attcggcgag gtttttcggt tcgtcggggt cgagcatatt cgggcggccg
    10981 tgtcccacgt cgcagaacgg gcagcggcgg gtgcagatgt cgcccataat catgaaggtc
    11041 gccgtgcctt tgctgaagca ttcgccgatg ttggggcagg aggcttcctc gcaaacggtg
    11101 tgcatttttt gttcgcgcaa aatgtctttg atttcaaaga atttgcgcga tggaagtttg
    11161 gcgcgtatcc attcgggctt ttttaatttt tcctgaaggg ggacgacttt gatggggatg
    11221 cgggcggttt tgtccgcgcc tctgagtttg atgccgcgtt tggggtcgtc ggttttgatt
    11281 tcactcattg ttgtctgctt tcggtgtgaa ttgtgtttca aggtgtgcgg taagtttggc
    11341 ggcgacttcg tccggcgtgg ggcagggttg gacaaaatcc gcgatttgcg tcatttccat
    11401 accggcgtag ccgcaggggt tgatgtgggt aaacgggctt aaatccatat tgacgttgag
    11461 cgcaagcccg tgatagacgg agccgttttt gatacgcagc cccagtgagg cgattttgcg
    11521 ttctccgaca taaacgccgg ggcgtttggg gtctgccgcc gcttcgatgc cgtattctgc
    11581 caatgtggcg atgatgctgt tttcaagcgc ggaaacgatg ttgcggacgc tggttttgcg
    11641 ccgtttgaaa tcaatcatcg tataaacgac caattgcccg ggcccgtgat aggtaatctg
    11701 cccgccccgg tcgatttgga cgacgggaat gtcgtcgcga atcagcaggt gttcgggttt
    11761 tcccaccagt ccttgtgtga acacgggcgg gtgttcgacg acccacagtt cgtcttcggt
    11821 gtcagcattc cgtccggcat taaaggtttt catcgcttca aaagtcggca gatattcgac
    11881 caaacctttg tgtatgattt tcatctcaaa gtaccacttt gaccagttcg tgcgaagtca
    11941 gcgcgcggta gatgttgtcc aattgttctt ggttttcaac ctttacctga acggtggcgc
    12001 cggtatagtt gcctttgctg ctcggacgcg tggtgatgtg gtgcgcctgc gtgtcggggg
    12061 cgtggaggcg gacggtgtcc aataccgcct gctcgaactc gggatgaacc gcccccatta
    12121 cttttaatgg gaaggtgcag ggaaattcga tgagggatgt tttgttttct tgttcggtca
    12181 tgatgtgctg ccttgttgtg tacggtatgc cgtctgaagg cgggtttgcc tttcagacgg
    12241 catcggatgt gcgttatttt agcctaaacc gcgataacag gctatcggga aggcggaggc
    12301 ttttttgacg gcgcggcggt tctgctatac tggcgcgcaa tattattttt agaagggtgg
    12361 tttttatgta tcggaggaaa gggtggggca tcaagccgtg gatgggtgcc ggtgcggcgt
    12421 ttgccgcctt ggtttggctg attttcgcgc tcggcgatac tttgactccg tttgcggttg
    12481 cggcggtgct ggcgtatgtg ttggaccctt tggtcgaatg gttgcagaaa aagggtttga
    12541 accgtgcatc cgcttcgatg tctgtgatgg tgttttcctt gattttgttg ttggcattat
    12601 tgttgattat tgtccctatg ctggtcgggc agttcaataa tttggcatcg cgcctacccc
    12661 aattgatcag ttttatgcag aacacgctgc tgccgtggtt gaaaaataca atcggcggat
    12721 atatggaaat cgatcaggca tctattattg cgtggcttca ggcgcatacg ggcgagttga
    12781 gcaacgcgct taaggcgtgg tttcccgttt tgatgaggca gggcggcaat attgtcagca
    12841 gtatcggcaa cctactgctg ctgcccttgc tgctttatta tttcctgctg gattggcagc
    12901 ggtggtcgtg cggcatcgcc aaactggttc cgaggcgttt tgccggtgct tatacgcgca
    12961 ttacgggtaa tttgaacgag gtattgggcg aatttttgcg cgggcagctt ctggtgatgc
    13021 tgattatggg tttggtttac ggcttggggt tggtgctggt cgggctggat tcggggtttg
    13081 caatcggtat ggttgccggt attttggttt ttgttcccta tttgggcgcg tttacaggac
    13141 tgctgctggc aaccgtcgcc gccttgctcc agttcggttc gtggaacggc atcctatcgg
    13201 tttgggcggt ttttgccgta ggacagtttc tcgaaagttt tttcattacg ccgaaaatcg
    13261 tgggagaccg tatcgggctg tcgccgtttt gggttatctt ttccctgatg gcgttcggac
    13321 agctgatggg tttcgtcgga atgttggcag gattgccttt ggccgccgta accttggtct
    13381 tgcttcgcga gggcgtgcag aaatattttg ccggcagttt ttaccggggc aggtaggcgg
    13441 ttccgaaaca tatttgaagc gggatacaac cctgcccggg tttaa
//
LOCUS       DAXHMX010000046        13224 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHMX010000046 DAXHMX010000000
VERSION     DAXHMX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.046"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(43..810)
                     /locus_tag="ACFM4S_000957"
     CDS             complement(43..810)
                     /locus_tag="ACFM4S_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6050664.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALTALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVYETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(898..1725)
                     /gene="mutM"
                     /locus_tag="ACFM4S_000958"
     CDS             complement(898..1725)
                     /gene="mutM"
                     /locus_tag="ACFM4S_000958"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6050665.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1777..2442)
                     /locus_tag="ACFM4S_000959"
     CDS             complement(1777..2442)
                     /locus_tag="ACFM4S_000959"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6050666.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRLLADAGFDIELGKFMVYLPPVS
                     SLGQIRFWQFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     IAD"
     gene            2620..4596
                     /locus_tag="ACFM4S_000960"
     CDS             2620..4596
                     /locus_tag="ACFM4S_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6050667.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIVRLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4664..5356
                     /locus_tag="ACFM4S_000961"
     CDS             4664..5356
                     /locus_tag="ACFM4S_000961"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6050668.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPAGETLCETLKNGVLLHD
                     ENETVYAADAVLESPSALLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            5882..7197
                     /locus_tag="ACFM4S_000962"
                     /pseudo
     CDS             5882..7197
                     /locus_tag="ACFM4S_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519747.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            7441..8097
                     /gene="cmk"
                     /locus_tag="ACFM4S_000963"
     CDS             7441..8097
                     /gene="cmk"
                     /locus_tag="ACFM4S_000963"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6050669.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            8253..9938
                     /gene="rpsA"
                     /locus_tag="ACFM4S_000964"
     CDS             8253..9938
                     /gene="rpsA"
                     /locus_tag="ACFM4S_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6050670.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9949..10263
                     /locus_tag="ACFM4S_000965"
     CDS             9949..10263
                     /locus_tag="ACFM4S_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6050671.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(10614..11021)
                     /locus_tag="ACFM4S_000966"
     CDS             complement(10614..11021)
                     /locus_tag="ACFM4S_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC6050672.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            11169..12278
                     /locus_tag="ACFM4S_000967"
     CDS             11169..12278
                     /locus_tag="ACFM4S_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6050673.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPAKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGTAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFDLMHEGKSIRAVIHY"
     gene            12287..13114
                     /gene="fghA"
                     /locus_tag="ACFM4S_000968"
     CDS             12287..13114
                     /gene="fghA"
                     /locus_tag="ACFM4S_000968"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6050674.1"
                     /translation="MKLIEQHQIFGGLQQVWAHYAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGF
                     YLNATEQPWVTNYQMYDYILNELPSLIEEKFPTNGKRSIMGHSMGGHGALVLALRNRE
                     RYQSVSAFSPILSPSLVPWGEKAFSAYLGKNREKWQQYDADSLIRQGCKVQGMRIDQG
                     LEDEFLPTQLRTQDFIETCRAANQPADVRFHKGYDHSYYFIAGFIGEHIAYHAAFLK"
BASE COUNT         3459 a         3667 c         3057 g         3041 t
ORIGIN      
        1 tccgcaaaag cgaatcatac tgccacattc cggcaaccgg tttcagacag cgatatccgc
       61 atcgtcggcg acaacggcac ggatgctttc ttcgatttta tcttttaaag cataacggtc
      121 ttcgctttcc gccgcatccg ccacgcaaac gaaatcgact tttatcgtta attttttcat
      181 agacacaatg cgccacaggc aggtcggcaa accgacatcg gcatatgagg gacgagccgt
      241 cctttttccc gtttcgtcat aataacgcag cgcgaccgcc aaaacctttg cccccgcatc
      301 aatggcggat tggaacagcg cggctttgaa cggcaggagc cccagtccgg aggaagtccg
      361 cgcttcgggg aaaaaactga cgttttgacc gcgttgcaag gtttcgtaga cggcgcggtt
      421 aatcggttcg atgtcgcgcc gcgaattgcg gttgatgaac accgttcccg cgttctgccc
      481 catcttgccc aataccggcc agcttttgat ttcctgcttg gcgataaagc tgctcggata
      541 aaccgcgctc atcgcgaaaa tatccagcca ggacacgtgg ttggcggcaa ccaagacacc
      601 gttcggatgt tcgggtgcgg gcctgcccac ctccaatccg atatccaaag ccgtcagagc
      661 ccccctgccc aactcgatta ccgcccgatt gcgcgactcg gggcagccgc cgtcaatacc
      721 gcgcaggttt ttgccggttt tgaatagcca gaccgtcaag cggcacaagc ggcgcagacg
      781 tgtaaaaaat gaagctttat ttgaagacat gaacaattct ttcccgataa tcggtgtttg
      841 ttgttgaaac gttcggacgg cataccgata aaatgccgtc tgaaaccgtt ttccgcccta
      901 tttctgacaa ttcgggcagt aaaacgtgcc gcgctgcccc aaagtttctt tcacaaccaa
      961 accgccgcac tgggggcacg gctgattgtg ccgcccgtac accgtatatt cctgttggaa
     1021 gtagccgctt ttgccgtcgc tgtccacaaa atccctcaag gtactgccgc ccgtttcaat
     1081 ggcgcgccgc aacaccgctt tgacggtttc aaccaaaagc gcacactctt tctttttcag
     1141 acggttggca ggacggtggg gcgaaatgcc cgctctgaac aggctctcat tggcataaat
     1201 attgcccaca ccgaccacga ccgcattgtc catcagggca agtttgaccg cgcgcttctg
     1261 cgccttcagc ctcacataca gataatccgt gcaaaatgcc tccgacaaag gctccggccc
     1321 cagtttttcc aacagcggat gatgttcttc gattccctca taccaaagta tcgcgccgaa
     1381 ctttctcgga tcgcggtaac gcatgaccgt gccgtctgaa aacacaatat cgacgtgatc
     1441 gtgtctgtcc ggcctgccga tacgtccgtc cgacggcgta aaaatccgca agctgcccga
     1501 catccccaag tgaatcagca gcacgcccgt ttgaaagcgg ataagcaggt atttcgccct
     1561 cctgccgcag gacaacacct gccggccgga caaaatctcc cccaaatcgg gattaatctg
     1621 ccagcgcagc ttcaattggc gcaataccac ggcttccacc gttttccctt caatatgcgg
     1681 cgcgatgccg cgcaacgtcg tttccacttc cggcaattca ggcataaccc ctcccgacat
     1741 ttcttctgac agatgccgtc tgaaagacgg ctgtccctaa tccgcaatcc ttgccgcacc
     1801 cgccgcaagg gctttgccgc ccaaataccc gtcccacgcg ggcaggggcg ttacgcccgc
     1861 cgcccttttg accaaaacca gaccgtacac tgcggcacat tgcggccacc aacggtcgcc
     1921 cgccttttcc ataaactgcc aaaagcgtat ttgcccgagc gacgaaacgg gcggcagata
     1981 caccataaat ttcccaagtt caatatcgaa gccggcatcg gcaagcagcc ttttcaactc
     2041 gggcagcggc agacaaaacc gtttttccgg caggcgttcg ccgtcaaacc aacggctgaa
     2101 tccccagagc gaatacggat tgaaacccgt cagcatcaaa cgtccgcacg gtttcaatat
     2161 ccggtgcgct tccgacagga tttgcgaagg aacaccgcct tcaagcgtat gcggaaaaag
     2221 cagcatatcc gcagaaacat ccgccaaagc catattctcc gccgacatcg acatatctcg
     2281 cggcacacag acaacatctt cagacaggct cagccacgga ccgcccacct gaaccgcaca
     2341 cattcccgaa aaacggtatg aatctagata ccgcccgaag aagtcctgtt ccaattttgc
     2401 aacataccgc cccatcgccg tatcttcaaa ccatgcatcc atattgcgtc cgtttcaaac
     2461 agattgcctg ccgatattct attccaaaca gaatttctgt caaaaaacac atcggccgcc
     2521 cacttccgaa tccgcataaa gttcctgtaa aacttgacgc tttttcagtc aaacagtacc
     2581 atcggacgat aaaatatgtt tattccgccg atataaatca tgtccaaact caaaaccatc
     2641 gccctgaccg cgtcaggtct gtccgtttgt ccgggtttcc tatacgccca aaacacctca
     2701 tcacaccaaa tcggtttggc gattatgcgc ttaaactctt caatactcga cctgcccccg
     2761 acaaaacaat atttccaatc cggcagcctg tggagcgagc tgcgccaagg cttccggatg
     2821 ggcgaagtca atcccgaact ggtacgccgc cacgaaagca aattcatcgc aagccacagc
     2881 tatttcaaca gggtcatcaa ccggagtaga ccctatatgt accatatcgc caacgaagtc
     2941 aaaaaacgca atatgcccgc cgaagccgcc ctgcttccct tcatcgaaag cgcgttcgtc
     3001 accaaagcca aatcacacgt cggcgcatcg ggcctgtggc agttcatgcc cgctaccggc
     3061 aggcattacg gcctggaaaa aacaccggtt tacgacggca ggcacgacat ttacgccgcc
     3121 accgatgccg cactcaacta tctgcaatac ctctatggac tgttcggcga ctggccgctc
     3181 gcctttgccg cctacaactg gggtgaaggc aacgtcggac gcgccatcaa ccgcgcccgc
     3241 gcccaagggc tcgaaccgac ctacgaaaac ctgcgtatgc ccaacgaaac gcgcaactat
     3301 gtccccaagc tgctcgccgt gcgcaacatt attgccactc cccaatcttt cggtatgaat
     3361 atcagcgaca tagacaacaa accctatttt caggcagtcg aaccggatcg tccgctcgac
     3421 aacgaagcca tcgtccggct tgccggcatc acgcaaagcg agctgctcgc cctaaacccc
     3481 gcattcaacg tccccgcgtt tatccccaaa agcaaacgca aactgctgct tcctgtcgca
     3541 tccgtccaaa ccttccaaag caactacctc aacgccgcac ccgacagcct gttttcatgg
     3601 gaagtctata cgcctgccgc caaaaccagc ctgtccgaca tctcgacggc aaccggcatg
     3661 agcattgccg acatcaaacg cctcaacaac ctgaacggca accttgtcaa cgcaggacgc
     3721 agcatccttg tcgccaagaa cggcaaaacc cttcagacgg catcggaatc cgtcgtttcc
     3781 atcgacatcg acaatacgcc cgacacctac cgttccaata tgccggcagg cacggtgaac
     3841 gtcggcattg cccgaatccg acccgccgcc gcacagacag cggacattac cgtcgcacct
     3901 ttgccgcaga aaaccgtccg tacggaaccc gatccccttg tccgtattgc cgaacctgcc
     3961 cttgcgacag ccgcagcgca acctcaaacc gaaaaacaga ccgccatgcc gtctgaaacc
     4021 caaaccgcaa cactcgcgca gatcatcccc caaaacgaca tgcaggcggc agacgaactc
     4081 atgcagcttg ttgcccgaaa caacctgcgc cgacaggctg aagaaaccat ctccgccgtc
     4141 atcggcacgc ctgacacagt tgccgaacac aacatttcct catctccgca acataccgtt
     4201 gccgccgacg gcaaacgccg ggcacgtttg gaaacgcgcg tagccaaagc tgccgacggc
     4261 gaagccgaaa tctccccgct ccatgccagc atccaccgcg ttgtagaagg cgacaccctg
     4321 ttcaacattg ccaaacgcta caacgtcagc gtagccgacc tgattgtcgc caacaacatc
     4381 aaaggcaaca ccatccaaaa aggacaggta ctccgcctgg cgcaggcagc ccctgcccaa
     4441 acccgtattg aaaaagtatc ctacaccgcg cgcaaaggcg acaccttcaa aagtatcgcc
     4501 gcgcgcttca atatccatat cgacgacatc cgccggctca atcccaacct gaacaccatc
     4561 aatccgggac agagggtcaa actgattgga agctgattcg gatacggcac atgacaggac
     4621 tttctcagtc ctgtcttttt catcccacat ttcccatacc atcatgaaac ttatcaaata
     4681 cctgcaatat caaggcatag gaagccgcaa gcagtgccaa tggctgattg ccggcggtta
     4741 tgttttcatc aacggaacct gcatggacga caccgatgca gacatcgatt cctcatccgt
     4801 cgaaacgttg gatattgacg gggaagcagt aaccgtcgtt cccgaaccct atttctacat
     4861 catgctcaac aagcctgaag attacgaaac ttcgcacaaa cccaagcact accgcagcgt
     4921 attcagcctg ttccccgaca atatgcggaa catcgatatg caggcggtcg gcaggctgga
     4981 tgcagatacg accggcgtat tgctgattac caacgacggc aaactgaacc acaacctgac
     5041 ttcgccgagc agaaaaattc ccaagctata cgaagttaca ctcaagcacc ctgccggcga
     5101 aacgctttgc gaaaccctga aaaacggtgt cctgctccac gacgaaaacg aaacggttta
     5161 cgccgccgat gccgtattgg aaagcccgtc cgcattgctc ctgaccatta ccgagggaaa
     5221 ataccaccaa gtcaaacgta tggtcgccgc cgcaggcaac cgcgtgcaac accttcatcg
     5281 ccggcgattc gcacatttgg aaacagaaaa cctgaaaccc ggcgaatgga aatttatcga
     5341 ttgtccgaaa ttctaaaata acatccaaga attccattta tattcatcca catactcatt
     5401 aaatatatgg tttaacccaa tttaacgcaa aaaatcaatt tacgatataa tccatttgtc
     5461 tgagtaacgc ctgttcaagc aggcttatga gtaagacgtt ttccccgtaa tgtgttttgc
     5521 catctatact tctccccttg tatagatggt tttttatttc aaattccctc tctttcatca
     5581 ttattaaaat gctatcatta atgtgattaa caaaatttca tttccataca ccgatgtaga
     5641 agaataacaa attaatctat ttataaactc cgattcttcc tcatcgggtt atactcatca
     5701 gaaatactca ctgaaatata tgccttttac atggaaaatc actagacagt tgaaaaacaa
     5761 tcagtcaatt cctgttaaaa aacgcgtgta gaatttccgc tttatctcaa tcggacacgg
     5821 tttcaatatg tcttcccctt caaataccaa tcgtcaaacc tgatccagcc gattaaccta
     5881 tatcctgact gttgccggcg cgactgtcgg cttcggcgcg acgtggcgtt tcccgtattt
     5941 ggtcggtgaa aacggcggcg gcgcgtatgt gtttttattc tgtatcgcca tgctcgttat
     6001 cggcatcccg atgattttgg tggaaaacgt catcggacgg cgcaaaggcg tgaacgcgct
     6061 ggatgcgttc ggcggtccga tgaacggcaa acccattgct aaaatttgga aactggtcgg
     6121 ctggatgggg ctgctcggcg cgttcggcat catggcttat tacatggcac tcggcggctg
     6181 ggtaatcagc tatatcgtta atattattgg aggaaatttg aatatttcca gccccatcga
     6241 cggtgtggtt acaaaaggct tctttgccga acacattgaa acagcccttg ggaaattgcg
     6301 ttttatacgc tgctttttgt cgccgtgaac caatggattt tggtcaaagg cgttatcggc
     6361 ggcattgaaa aagcggcaaa atagctgatg ccgctgctgt ttttgttcct aatcgcgatg
     6421 gtcgtccgca acgttaccct tccgggcgca atggaagggg ttgctttcta tctgaaacct
     6481 gatttcagca agattaccgc cgaactgttc gtcttcgttt tggggcaggt attttttgcc
     6541 ctgagcctgg gtttcggcgt gatgattacc ttgtccagct atttggataa aaacgaaaat
     6601 ctggttcaga cggcagttat cacggcaatt accaatacca tcatcgccat acttgcgggc
     6661 tttatgattt tcccgtcgct cttcagcttc ggcgttgccc ccgattccgg cccgactttg
     6721 gtattccaaa gtttgccgat tgtattctcg catatgtggg caggatctgt gttcgccgtc
     6781 attttcttct cgctgctcct gattgccgcg ttgacaactt cgctgaccat ttatgaagtg
     6841 ttgattacga ccattcagga aaaaaccaaa atccgccgta ccgccgcgat tacgattgta
     6901 ttggctgcca tcttcatttt cggcaacatc ccgtccattc tgagctatgg tccgtggaaa
     6961 gacgtttccg tgttcggcaa aaatattttc gatgccttcg actacatcag cggcaacatc
     7021 ttgtttatgc tgaccgcgct cggttccgcg ctgtttgccg gttttgtgat gaaggacgaa
     7081 gcgaaggacg aattgcttta taaaggcaac catacgacgg tcaatatttg gtttgcttat
     7141 gtgaaatatc ttgtgccgct ggtgattctg ctgattttcg tcagcaacct gttctaatct
     7201 gcagcaatcg atgccgtctg aaggtcatac ctctttcaga cagcattgcc ttgatgcccg
     7261 ccacaatacc cgcaacgccg gaatccgaac cggcttgccg gcttctgtcg gatgttttcg
     7321 ggcaggcggc gttccgtctg cttagacaat cgtcctttaa aacaggtaga atccgcccca
     7381 acgggaaaca catccttcag acggcaaaac ccatacccca aaccatcagg aatccccctt
     7441 atgaacagac aaaaagtcat cgccatcgac ggcccgggcg catcgggcaa aggcacggtt
     7501 gccgcccgcg ttgccgccgc attgggatac gattatctcg ataccggcgc actctaccgc
     7561 ctgaccgccc tatatgcaca aaaacaaggc gtgggatggc acgatgaaga aaacgtttcc
     7621 gaactggcaa aaaaactgcc cgccgtattt tcaggcagcc gcatcctgct cggcggcgaa
     7681 gacgtttcag acggcatccg gacagaagcc atcggcatgg gcgcatccgc agtcgcacag
     7741 ttgcctaaag tccgcgccgc cctgctgcaa cgccaacgcg attttctgac cgaaaaagga
     7801 ctggtcgccg acggacggga caccggatcg gtcgtcttcc cccaagccga acttaaaatc
     7861 ttcctgacgg cagaatccaa aatccgtgcc gaacgccgcg ccaaacaaat cggcatcccc
     7921 tgcgaaggtt tggcattcga gcgcatcctg tccgacatcg aagccagaga cgaggcagac
     7981 cgaaaccgca aggttgcccc cctgaaacaa caacccgatg ccctgctttt ggatacgagc
     8041 cgcctgacta tagaagaaac tgtaaaaaaa gtgcttgatt ggtatcgtaa agtttaaatt
     8101 ttcaggtata atcgcccgca ttatgtttca gacggcataa aaatccccca tatgccgtct
     8161 gaaaccttat ttacccgtct gccctgccaa ggacggcaga cacccaccaa cccaccccgc
     8221 accccttggc ggtgtaccga aaagagttat atatgtctat ggaaaatttt gctcagctgt
     8281 tggaagaaag ctttaccctg caagaaatga acccgggtga ggtgattacc gctgaagtag
     8341 tggcaatcga ccaaaacttc gttaccgtaa acgcaggtct gaaatcagaa tccctgattg
     8401 atgtagctga attcaaaaac gctcaaggcg aaattgaagt taaagtcggc gacttcgtta
     8461 ccgttaccat cgaatccgtc gaaaacggct tcggcgaaac caaactgtcc cgcgaaaaag
     8521 ccaaacgcgc tgccgattgg attgccctgg aagaagccat ggaaaacggc gacatcctgt
     8581 ccggcatcat caacggaaaa gtcaaaggcg gcctgaccgt tatgattagc agcatccgcg
     8641 cattcctgcc gggttctttg gtcgacgtac gtcctgtaaa agacacttct cacttcgaag
     8701 gcaaagagat cgaattcaaa gtgatcaaac tggacaaaaa acgcaacaac gtcgttgttt
     8761 cccgccgcgc cgttctggaa gccactttgg gtgaagaacg caaagccctg ctggaaaacc
     8821 tgcaagaagg ctccgtcatc aaaggcatcg ttaaaaacat taccgattac ggtgcattcg
     8881 ttgacttggg cggcatcgac ggtctgttgc acatcaccga tttggcatgg cggcgcgtga
     8941 aacacccgag tgaagtcttg gaagtcggtc aggaagttga agccaaagta ttgaaattcg
     9001 accaagaaaa acaacgcgtt tccttgggta tgaaacaact gggcgaagat ccttggagcg
     9061 gtctgacccg ccgttatcct caaggcaccc gcctgttcgg caaagtatcc aacctgaccg
     9121 actacggcgc attcgtcgaa atcgaacaag gcatcgaagg tttggtacac gtctccgaaa
     9181 tggactggac caacaaaaac gtacacccga gcaaagtcgt acaactgggc gacgaagtcg
     9241 aagtcatgat tttggaaatc gacgaaggcc gccgccgtat ctctttgggt atgaaacaat
     9301 gccaagccaa tccttgggaa gaatttgccg ccaaccacaa caaaggcgac aaaatctccg
     9361 gcgcggttaa atccattacc gatttcggcg tattcgtcgg cctgcccggc ggcatcgacg
     9421 gtttggttca cctgtccgac ctgtcctgga ccgaatccgg cgaagaagcc gtacgcaaat
     9481 acaaaaaagg cgaagaagtc gaagccgtcg tattggcaat cgacgtggaa aaagaacgca
     9541 tctccttggg tatcaaacaa ctggaaggcg atccgttcgg caacttcatc agcgtgaacg
     9601 acaaaggttc tttggttaaa ggttccgtga aatctgttga cgccaaaggt gctgttatcg
     9661 ccctgtctga cgaagtagaa ggctacctgc ctgcttccga atttgcagcc gaccgcgttg
     9721 aagatttgac caccaaactg aaagaaggcg acgaagttga agccgtcatc gttaccgttg
     9781 accgcaaaaa ccgcagcatc aaactttccg ttaaagccaa agatgccaaa gaaagccgcg
     9841 aagcactgaa ctccgtcaat gccgccgcca atgcgaatgc cggcaccacc agcttgggcg
     9901 acctgctgaa agccaaactc tccggcgaac aagaataagg ttgcagacat gacaaagtct
     9961 gagttaatgg ttcgtttggc agaagtgttt gccgccaaaa acggcacgca tcttctggca
    10021 aaagacgtag agtacagcgt aaaagtcttg gttgacacca tgactagatc gcttgcccga
    10081 ggtcaacgca tcgaaatccg cggtttcggc agcttcgatt tgaaccatcg tcctgcccgc
    10141 atcggtcgca atcccaaaac cggcgagcgt gtggaagtac ctgaaaaaca tgtaccccac
    10201 ttcaagcccg gtaaagaatt gcgcgagcgg gtcgacttgg ctttaaaaga aaatgccaat
    10261 taaaccttag catcaaaacg ccgctgttac gcggcgtttt ttctgtggtt taacttcatc
    10321 cgctgcttca ataccttgag ccaagcaagc aacggattag agcgtggatt tttttatagt
    10381 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagagaac aattctctaa
    10441 ggtgctgaag caccaagtga atcggttccg tactatttac actgtctgcg gcttcgtcgc
    10501 cttgtcctga tttttgttaa tccactatat cattgcttac aatccgcttt ttaaacaaca
    10561 aatttttgat ttctattacg aacaggacaa aaatcctgct tattgcacta aaactaagcc
    10621 gtttcaggaa tttgcggcaa atttacggct tttaccgaac ctaatgcttt cgctttttgg
    10681 taaaacgcca atttgtattc aagcaaatct aaatagcgtt ttaattcggc aatttgacac
    10741 ttcacatttt ctatttgatt ttcaaacaag gaaaggcgtt cttcaatggt atcgtcgcca
    10801 atgacggtac attccgcaaa gcgtttgatg tcttttaagc tcattcccgt atttttcaag
    10861 cattgcaata agcccaacca ttgcaaatcg ttatcggtaa aacagcggtt accgtattca
    10921 tcacgtccga tattaggcaa caagccttct ttgtcgtaaa aacgtagggt gtgggcggag
    10981 atgcctattt tttcggcggc tttggcagtg gtataagtca ttttccctcc ttctaaacaa
    11041 aaacagtaaa aaacacttgc cttagagtta actctaaagt gtaaactgtt gctatgttgc
    11101 tcaggtaagg caactttgtc aatgaattaa gaggaaagac aatggaaatg aaacaaaccg
    11161 attcaaccat caaatctcgt gcggcggtgg cattcgcccc taaccaaccc ttacaaattg
    11221 tggaaatcga cgtagaaatg ccgcgtaaag gcgaggtgtt aatccgcaat acccacactg
    11281 gcgtgtgcca tactgatgcg tttacgttat caggaagcga tcctgaaggc gtattccctg
    11341 tggtgcttgg acacgaaggt gcgggtgtgg tcgttgctgt gggcgaaggt gtgtcaagcg
    11401 taaaaccggg tgatcacgtg attccgcttt acaccgccga atgtggcgaa tgtgagtttt
    11461 gttgttcagg taaaaccaac ttgtgcgtct cagtgcgtga tacacaaggt aaaggcttaa
    11521 tgccggacgg cacgacgcgt ttttcttatc aaggtcagcc aatctatcac tatatgggct
    11581 gttcgacttt cagtgaatac tccgttgttg ccgaagtttc actggcgaaa atcaaccctg
    11641 aagccaacca tgaacaagta tgtttgctcg gctgcggcgt taccacaggt attggtgcgg
    11701 tacataatac ggcaaaagtg caagaaggcg actctgttgc cgtgtttggc ttaggcgcga
    11761 ttggtttggc ggtggtgcaa ggtgcgcgtc aagccaaagc cggccgtatt atcgccattg
    11821 ataccaatcc cgcaaaattc gagttggcaa aacagtttgg tgcaaccgat tgtttgaacc
    11881 cgaacgatta cgataaaccg atcaaagatg tgttgttaga catcaacaaa tggggcatcg
    11941 accacacctt tgaatgtatc ggcaatgtaa atgtaatgcg tcaggcatta gaaagcgcac
    12001 accgtggttg gggacaatcc attatcatcg gcgtagcggg tgctggccaa gaaatttcca
    12061 ctcgtccgtt ccagttggta accggccgtg tttggaaagg cactgcattt ggcggcgtga
    12121 aaggccgctc cgagcttccg aaaatggtgg aagattcaat gaaaggcgac atcgagttag
    12181 aaccgtttgt gacccacaca atgacgctcg atcaaatcaa tgaagccttt gatttaatgc
    12241 atgaaggtaa atcgatccgc gctgttattc attactaagg tacgcgatga aactgattga
    12301 gcaacatcaa atttttggcg gtttgcagca agtttgggcg cattatgccc aaacgctgca
    12361 gtgcgaaatg aaatttgccg tctatttgcc aaataatccg gagaatcgac cgcttggtgt
    12421 gatttattgg ctttccggct tgacttgtac ggaacaaaat ttcattacca aatcaggttt
    12481 tcagcgttat gcggcagaac atcaagtgat tgtggttgcc cccgatacca gccctcgcgg
    12541 agagcaagtg ccgaacgatg cggcttacga tttagggcag ggtgcgggct tttatctgaa
    12601 tgcgaccgag cagccttggg tgacgaatta tcaaatgtat gattatattt tgaatgagtt
    12661 gcctagtctg attgaggaaa aatttcctac caacggcaaa cgttccatta tgggacattc
    12721 aatgggcgga catggcgcat tggtattggc actgcgaaac cgggaacgtt atcaaagtgt
    12781 ttctgccttt tcgcctattt tatcgccaag cctcgtgcct tggggagaaa aagccttttc
    12841 tgcctatttg ggaaagaatc gcgaaaaatg gcagcaatat gatgccgact cgctcatccg
    12901 acaaggctgt aaggtgcaag gtatgcgcat tgatcagggc ttggaagatg agtttttgcc
    12961 gacacaattg cgtacccaag attttatcga aacctgtcgt gcggcaaacc aaccggccga
    13021 tgtgcgcttc cacaaaggct atgatcacag ctattacttc atcgccggtt ttattggcga
    13081 gcatattgcc tatcacgcgg catttttgaa gtaagccaaa gagcgttcag tgttcaaagc
    13141 agttttggga tagccggcac gagggcggta agaagtgccg gcataaacgt atgccgtctg
    13201 agccggaaga agccgactct acgg
//
LOCUS       DAXHMX010000047        13095 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHMX010000047 DAXHMX010000000
VERSION     DAXHMX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.047"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(308..1204)
                     /gene="argB"
                     /locus_tag="ACFM4S_000969"
     CDS             complement(308..1204)
                     /gene="argB"
                     /locus_tag="ACFM4S_000969"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6050675.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1374..2504
                     /locus_tag="ACFM4S_000970"
     CDS             1374..2504
                     /locus_tag="ACFM4S_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6050676.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2575..3426
                     /gene="lgt"
                     /locus_tag="ACFM4S_000971"
     CDS             2575..3426
                     /gene="lgt"
                     /locus_tag="ACFM4S_000971"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6050677.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3509..4171)
                     /locus_tag="ACFM4S_000972"
     CDS             complement(3509..4171)
                     /locus_tag="ACFM4S_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6050678.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4269..6062)
                     /locus_tag="ACFM4S_000973"
     CDS             complement(4269..6062)
                     /locus_tag="ACFM4S_000973"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6050679.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6301..7410
                     /gene="proB"
                     /locus_tag="ACFM4S_000974"
     CDS             6301..7410
                     /gene="proB"
                     /locus_tag="ACFM4S_000974"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6050680.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPAKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     NRTDFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDRIEHINHEIIEMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGVAGIEGHFSRMDTVTVYSNATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7423..8685
                     /locus_tag="ACFM4S_000975"
     CDS             7423..8685
                     /locus_tag="ACFM4S_000975"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6050681.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRLDLEAAAGKIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIKAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIIRGVGQVRE"
     gene            complement(8757..11789)
                     /locus_tag="ACFM4S_000976"
     CDS             complement(8757..11789)
                     /locus_tag="ACFM4S_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6050682.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            11954..12634
                     /locus_tag="ACFM4S_000977"
     CDS             11954..12634
                     /locus_tag="ACFM4S_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6050683.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            12663..13022
                     /locus_tag="ACFM4S_000978"
     CDS             12663..13022
                     /locus_tag="ACFM4S_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6050684.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT         2866 a         3743 c         3379 g         3107 t
ORIGIN      
        1 tctaaagaaa ccttttttcc cgataagttt ccgtgccgac aggtctagat tcccgcctgc
       61 gcgggaatga cgaaatttca aagttatggc gttatcggaa aaacagaaaa tcaaagccgg
      121 agaatttatc ccaaacaact ggatttcaaa aaaaccagat tcccgccttc gcgggaatga
      181 cggattttag gtttctgttt tggtttctgt tttcgcggga ataacaactt agaaattgcc
      241 cgaaacccca aaaacagaaa ccggacaaac gggttttccg ccaaagccgg cattttccga
      301 ctttgcttca ggcatcttcc ccaccgccca aaatcatcga accgataccg gcatcggtaa
      361 agatttccag caaaagcgcg ttgggcaacc tgccgtcgat gatatgcgtg gctttcacac
      421 cgttgacggc ggcttcgacc gcagaagcga ttttcggcag cataccgcca tacagcgtgc
      481 cgtcggcaat cagttcatca atccgtttcg gcgtgagttt ggtcagcaga ttgcccgttt
      541 tgtccatcac accggcgata ttcgtcatca tcaagagttt ttcagcgttt aattcttccg
      601 ccaatttgcc tgccaccaaa tcggcgttga tgttgaacgc ttcgcctttt tcacctacgc
      661 cgacgggggc gacgacggga atgcagccac gttctatcag ccctttaacc aaacctgtat
      721 caatgctttc caccgtaccg acctgtccga tgtccacgcc attctgttcg ggcgtatcga
      781 tcaaaagttt cttcgcctta atgaaatgat cgtcgcgtcc gctcacgccg accgcgtgtc
      841 cgccatatgt gttaatcatc gacacgattt ctttattgac atgcccaccc aacaccattt
      901 cgacaatatc catcgcctct ttgtcggtaa cgcgcattcc ttggacaaac tccccctttt
      961 tgccgacttt ttcaagcatc gcattgatct gcggcccgcc gccgtgaacg atgacgggat
     1021 gaatgccgac cagcttcagc agtacgacat cgcgggcaaa cccttctttc aaggcaggtt
     1081 cggtcatcgc gttgccgccg tacttgatga cggcgaccga accggaaaac cggcggatgt
     1141 aaggcagcgc ttcggcaagg atacgcgctt tgtcggcggc ggaaataatg ttttcagact
     1201 ccataacggc tccctattgt tgacaatttc ggcttatgtt aacgccaaac gccgtctgaa
     1261 acaatcttca gacggcatcc ggcagacctt cgggcaggtt gaagccttgc gatacgggcg
     1321 gtaaaatcac agtttatccg gttcgagaaa ccggcgacag caaggacttt cccatgatta
     1381 aaatcagcac ccgcttcgat gccggctcgg tcgtcgtcaa agacctgacc gatccttcca
     1441 acatccgcct cgccctgcgt cccgacaacg cctccgattt tgcacaatgg ttctacttcc
     1501 ggctgcaagg cgcggcatat caaaactgca tcatgcattt tgaaaacgcg gcagaagccg
     1561 cctacccgaa aggctgggaa ggctatcagg cgtgtgcctc atacgaccgc cgcaactggt
     1621 tccgcgtacc gacttcctac gaaaacggcg tactgaccgt caatcatacg ccgctgtcca
     1681 acagcgtgta ttacgcctat ttcgaacctt actccgaaga acagcattta aacctcctcg
     1741 gcgacgcgca aggcagcggc ctgtgccgca tcgacgactt gggcagcacc gtgcaagggc
     1801 gcgacatcaa tctgctgacc atcggcaatc aggtcgaaag cgatctgaaa atctggatta
     1861 ccgcccgcca gcatccgggc gaaaccatgg cggaatggtt tgtcgagggg ctgctcggca
     1921 ggctgctcga tccgcaagac ccgacagcgc gcaccctgct cgaccgcgcc acgttctata
     1981 tcgtccccaa tatgaacccc gacggttcgg cactgggcaa ccttcgtacc aacgccgccg
     2041 gtgcgaacct caaccgcgaa tgggaaaatc cgactttgga aaaaagcccc gaagtgttct
     2101 tcgtgcgcga aaaaatgctg gaaaccggcg tggatttgtt cttggacatc cacggcgacg
     2161 aagggctgcc ctttatcttt gttgcaggaa ctgaaggcgt gccaaactac aatccgcgca
     2221 tttccgcgtt ggaaacacag ttcaaaaccg cccttttgaa cgccagcccc gatttccaag
     2281 acgaatacgg ttacgaaaaa gatgcgcccg gtcaggcaaa catgaccttg gcaacaaact
     2341 gggtcggcaa ccgtttcaac tgccttgcct atacgttgga aatgccgttc aaagacaatg
     2401 ccaacctgcc cgacgacgat ttcggatgga acggtcaacg gtcgttgcgc ttgggcgaag
     2461 ccgcgttgtc cgccatcctc aacatcatcg gcgatttgcg ctgaaaccct tattgcagaa
     2521 atgccgtctg aagcatcatg cctttcagac ggcatttgaa aacgaaagcc cgttatgatt
     2581 acccatcccc aattcgaccc cgtccttatc agtatcggcc cgcttgccgt ccgctggtat
     2641 gccctaagct acatcctcgg atttattctt tttacctttc tcggcagaag gcgcatcgcg
     2701 caaggcttgt ccgtttttac caaagaatcg ctcgacgact tcctgacatg gggcattttg
     2761 ggcgtaattt tgggcgggcg tttgggttac gtcctgtttt acaagttttc cgactacctc
     2821 gcccatccgc ttgatatttt caaggtatgg gaaggcggaa tgtcgttcca cggcggcttt
     2881 ttgggtgtag ttattgccat atggttgttc ggccgcaaac acggcatcgg cttcctcaaa
     2941 ctgatggaca cggtcgcacc gctcgttccg ctgggtctcg cttcgggacg tatcggcaac
     3001 ttcatcaacg gcgaactttg gggacgcgtt accgacatca acgcattttg ggcaatgggc
     3061 ttcccgcagg cgcgttacga agatgccgaa gccgccgcgc acaatccgct ttgggcagaa
     3121 tggctgcaac aatacggtat gctgccgcgt catccctcgc agctttatca gtttgcactt
     3181 gaaggcatct gcctgttcgc cgtcgtttgg ctgttctcta aaaaacagcg gccgaccgga
     3241 caagtcgcct cgctcttcct cggcggctac ggcatattcc gcttcattgc cgaattcgca
     3301 cgccaacccg acgactatct cgggctgctg accttggggc tgtcgatggg gcaatggttg
     3361 agcgtcccga tgattgtttt gggtatcgtc ggctttgtcc ggttcggcat gaaaaaacag
     3421 cactgaccgc ctgtgaaaaa taccgtctga aacatggaaa tccggtttca gacggcattt
     3481 ttacgtttgg ttcatggaaa caaacgcttc aattcttacc gcaacacgcc ttgtattttc
     3541 tgccgctgcc gcacggacag ggatcgttcc tgcctgtttt ttcgccttcc ctgcggacgg
     3601 tttgcggctt gttgatgacc gcctgccagt agcggtagat atccgccaat gcataaggca
     3661 actcggattc cagttccgcc agctcgcctt ctgtgaattg cagacggata gtgccgttgt
     3721 cctcttcgtc gtaaataccg cccaatgcca tgatgggata aaacaactct tcaaacgctt
     3781 catcatcgac ggcttcaaac caatcggtcg gcacaatatc caaaccgtaa agataagcat
     3841 tgcaccatgt gtaaaaatcg ctgccgccgt cttcgttttc atacagccac aaatcgggca
     3901 gttttttatc cgacatcgcg gcggttgttt ccatcgccat tgccaaaacc agccgttcga
     3961 tttcggaacg ttcggcggcg gtaaattgcg attcgtcgcc caacacttcg ggcagccagt
     4021 cgagcggtgt caatttgtcc ggcccgctca acagcgccgt cataaaacct tgaacctcgt
     4081 cgcaacgcat cgtgttgcct tgttcgcttt tggcatccaa caattcgctc aaccgccgtt
     4141 tggatgcttc ggtaaatttt tgggaatcca tcatttaacc ttttaaaatg ggtttaatac
     4201 ctaattataa tcgcaatatg ccgtctgaag cctttttctg cttcagacgg catcgtctgt
     4261 tttcacgctc aaatcgtacc gctgccttgc gctttcggtt tggcggcact aaattccagt
     4321 ttgctcaagg cggaaaggta ggctttggcg gtggcaacca aaacgtcggt atccgcgccc
     4381 tgaccgttga cgacgcggtt gccgcgtgcc agacggacgc tggtttcgcc ttggctttcc
     4441 gtaccttgcg tgacggcatt gacggaataa atctgcaaag tcgcgccgct ttgcgccacg
     4501 ctttcaatcg ctttgaaaat tgcatcaacc ggaccggaac cggttgcgga agcgcgtttt
     4561 tcttcgcctt tgatgctgaa cacgatgtcg gcgcgcggct cttcgccggt ttcggtgctg
     4621 attttttggg aaatgaattt gtagctttcg gtattcatat tgcccatttc gtccgacacc
     4681 agcgcgtgca ggtcttcatc gaagatttcg cgttttttgt cggcgagttc tttgaagcgt
     4741 gcaaatgccg cgttcagtgc ctcttcgctt tccaactcga tgcccaaatc cgccagcttg
     4801 gttttgaagg cgttgcggcc ggacaatttg cccaagctca atcggttagc cgaccaaccg
     4861 accgattcgg cagacataat ctcgtaagtt tcgcggtgtt tcagcacccc gtcctgatgg
     4921 atgcccgatt catgcgaaaa ggcattggca ccgacaatgg ctttgttggg ctgcacggga
     4981 tagcccgtaa tggtggacac cagtttggac gaaggcacga tttgcgtggt atcgatgccg
     5041 gtttccaagc cgaacaagtc gtggcgcact ttcaacgcca tcacgatttc ttcaaccgaa
     5101 gcattgcctg cacgttcgcc caagccgttg acagtacatt ccacctgacg cgcaccgcct
     5161 tttaatgcgg caagcgaatt ggcaaccgcc aagcccaaat cgttgtggca gtgtgccgac
     5221 caaacgactt tgccgccgtt gggcgttttg gcaatcagtt cgcggaaaaa ttcttcggtt
     5281 ttgtacggga tggaatagcc gacggtatcg ggaatattga tggtggtcgc gcccgcttca
     5341 attaccgcgc cgcagatttc ggcaaggaaa tcgatttccg aacgcaacgc gtcttcgcag
     5401 gaaaattcca catcgtcggt gtattcacga gcgattttca ccgctttgac cgccgcctca
     5461 atcacctgct tcggcttcat cttcaatttg tactccatat ggatggggct ggtggcgatg
     5521 aaggtgtgga tgcgtttttt cggcgcgggc gcaacggcct cacccgcctg acggatgtcc
     5581 cgctcgatgg cgcgggacaa tgaacagacc gttgatttgg taatggtttt cgcaatcgca
     5641 ttgaccgcct cgaaatcgcc cgggctggca gcggcaaaac ccgcttcgat gatgtccaca
     5701 cccaattttt ccagctggcg ggcgacgcgg attttttcct ctttggtcat agcagcgccg
     5761 ggcgactgct cgccgtcgcg cagggtggtg tcgaaaatga taacgcggtt ggcctgtgtc
     5821 atttctgttc tctccagaga ttcgtttttt tgtaaaaaag cattcaaatc cagcggcctg
     5881 ccttgcgatt cggcaagtgc agtcaatttt tgcagctgcg ccaaaggcag cgagccgcgc
     5941 gtgcgccatt tgtagatggc ggcagttgtc gcggcatttt cgggatcgtg ccgtttcaac
     6001 gcttcggcaa gcgcgttcac gccgccgaaa taggcgacca gacggtctat atctaattgc
     6061 atggtagtcc ttgtctaaaa tcaatctgcc cgcagcagaa atatggacag gattatacgc
     6121 attttagaca aaacggcaat atcaatttga aaaaatctca ataactcatc atagatttta
     6181 ttaattttat acattttgtc tattttattg caggcccagc gcaattgttg acaattgcgg
     6241 cagaacaggg caaattcgac agcaacaaac caaacgctac aaacgcaaag aacagaaaca
     6301 atgaaataca agagaatcgt atttaaagtc ggcacatctt cgattaccca ttcggacggc
     6361 agcctctcgc gcggcaaaat ccaaaccatt acccgccagc ttgccgcatt gcatcatgcg
     6421 ggacacgagc tggtcttggt ttcatccggc gcggttgcgg cagggttcgg tgcgctgggt
     6481 ttcaaaaaac gtccggccaa aatcgccgac aaacaggctt ccgccgccgt cgggcagggg
     6541 ctgctgatgg aagaatatac ggcaaacctg tcttcagacg gcatcgtgtc cgcgcaaatc
     6601 ctgctcaacc gcaccgactt tgccgacaaa cgccgctacc aaaatgccgg cggcgcgctt
     6661 tccgtgctgc tgcaacgccg cgcgattccc attattaatg aaaacgacac ggtttcggtt
     6721 gaggagttga aaatcggcga caacgacaca ttgagtgcgc aagtggcggc gatgatacag
     6781 gcagacctct tggtgctgct gaccgacata gacggtcttt acacgggcaa cccgaacagc
     6841 aatcccgatg ccgtacggct ggacagaatc gaacacatca accatgaaat catcgaaatg
     6901 gcaggcggcg cgggctcgtc aaacggcacg ggcggtatgc tgaccaaaat caaagcggcg
     6961 acgattgcga ccgagtccgg cgtaccggtc tatatctgtt cctcgctcaa acccgatgcg
     7021 cttgccgaag cggcagataa tcaggcggac ggctcgtttt tcgtcccccg cgccaaaggt
     7081 ttgcggacgc agaagcaatg gctggcgttc tattccgaaa gccggggcgg cgtttatgtg
     7141 gacgaaggtg cggaacacgc tttgtccgaa cagggaaaga gcctgctgat gtcgggcgtt
     7201 gccggaatcg aagggcattt ttcccgtatg gacaccgtaa ccgtgtacag caacgcaacc
     7261 aaacagcctt tgggcaaagg gcgcgtcctt ttcggctctg ccgccgccga agacctgctc
     7321 aaatcgcgca aagcgaaagg cgtgttcatc catcgggacg attggatttc catcacgccc
     7381 gaaatacgcc tgcttctgac cgaattttag aaaaggaaac ccatgtcaaa cacacaaaaa
     7441 cagcttgccc ttgccaaagc ggcaaaaaaa tccgtcaaca cggcggatac ggaagaaaaa
     7501 aaccgcgcgc tgcttgctat ggcggacagt ctggaagcgg cggcggcaga tattttggcg
     7561 gcaaaccgtt tggatttgga agcggcggca ggcaaaattc ccgaaagcat gaccgaccgc
     7621 cttttgttgg acggcaaacg catttgcgcg atggcggacg gcatcagggc ggttgccgcg
     7681 ctgcccgacc ccgtgggcga aatactggaa acctcgactc tgcccaacgg cttggaaatc
     7741 gtcaaaaagc gtgtggcgat gggcgttatc ggcattattt acgaaagccg cccgaacgtt
     7801 acgtccgatg cggcggcttt ggcactgaaa agcggcagcg cggtcgtact ccgcagcggc
     7861 aaagatgcat tccaatccgc acgcgccatc gttgccgcac tcaaagccgg tttggcgcaa
     7921 acccgcatcg accccgacgc gctccagttg attgaagaca ccgggcgtga gggcagttac
     7981 gaaatgatga gggcgaaaga ttatctagac ctgctgattc cgcgcggcgg ggcggggctg
     8041 atacgggcgg tggttgaaaa tgccgtcgtg ccggttattg aaacggggac gggcatcgtc
     8101 cacatttata tcgacaagga cgcggattgg gacaaggcgc tccgtatcgt gtacaacgcc
     8161 aaaaccagcc gtccgtccgt gtgcaactcg atggaagtgc tgctggtgca tgaagacatt
     8221 gctgccgact tcctgcccaa gctcgaacgg ctgttggttc gcgaccgtat aaaagccgga
     8281 ctgccgcccg tccgcttccg tttggatccg caggcggcac ggcatatcgg cggcgaagcg
     8341 gcgggtgcag acgatttcga taccgagttt ttagactaca tcctcgccgt gaaaaccgtc
     8401 gcttcggtcg aagaggcggt cgggcacatc gaaacgcacg gcacgcacca ttcagacggc
     8461 atcgttaccg aaaaccgcca cgctgcggac tatttcacga cccatatcga ttctgccgcc
     8521 gtgtatgtca acgcgtccac gcgctttacc gacggcggcg aattcggctt gggttgcgaa
     8581 atgggcatct ccacgcaaaa actccacgcc cggggtccga tgggtttgaa agagctgaca
     8641 agctataaat acatcattcg gggcgttggg caggtcaggg aataatccgc attcaaacgt
     8701 ccgataaaaa tgccgtctga aacagtcttc agacggcatt tccatttgca aaaaaatcaa
     8761 gcattgtcca aggggacgag aatcgtacgg ttgccgttgt gttccggcgc ggatacgatg
     8821 ccttccgcct ccatctggtc aatcagccgc gcggcgcggt tgtagccgat acgcaatgcg
     8881 cgctgcacgc cagaaatgct ggctttgcgc gttttcaaaa caacggacac ggcctcatcg
     8941 tacatcggat cggtttcgcc gtcgccgctg cgcccgatgc cgggcagctc ttcgctgccg
     9001 ccgccgctca aaatatcgtc aacatagtcc ggttcgccaa actgcttcag atattcgacc
     9061 acgcggtgca cctcttcatc cgaggcaaac gcgccgtgaa cgcgctgcgg ataggcagta
     9121 cccggcggca ggaacagcat atcgccctga ccgagcaggt tttccgcgcc catttggtcg
     9181 agaatcgtgc ggctgtcgat tttgctggac acttggaacg cgatacgcgt cgggatgttc
     9241 gccttaatca gacccgtgat gacatcgacg ctggggcgtt gtgtggcaag gataagatgg
     9301 atgcctgccg cgcgggcttt ttgggcgagg cgggcaatca gttcctcgat tttcttgcct
     9361 gccgtcatca tcaaatcggc aaactcatcg accaccacca cgataaacgg cagtttttcc
     9421 aaaggttcgg gatcgtcggg cgtgaggctg aacgggttgc cgattttctc cccccttgct
     9481 gcggcttcgg cgattttttg attgaaaccc gcaagattgc gcacgcccat aaagctcatc
     9541 aggcggtagc gtttttccat ttcgttaaca caccagttca gcgcgtttgc cgccagcttc
     9601 atatcggtaa cgacaggggc gagcaggtgc gggatgcctt cgtaaatgct caattccagc
     9661 attttcggat cgatcataat catacgcacg tcttccggcg cggctttgaa aagcatagac
     9721 agaatcatcg cgttgacacc caccgatttg cccgaaccgg tcgtgccggc aaccagcaaa
     9781 tgcggtgctt tgcccaagtc ggttacgacg ggctgtccgg tgatgtcctg accgagcgcg
     9841 agcgtcagct tggatttgga ttcggcaaac tcgggcgaat tgaagatttc gctcaggcgt
     9901 atcatttggc gtttcgggtt cggaagttcc aaacccatac aggttttgcc gaggatggtt
     9961 tcgacaacgc ggatggaagc cacgccgagc gaacgcgcca agtctttttc cagatttaga
    10021 acggaattgc cgcgcacgcc gacatcgggt tcgatttcat aacgcgtaat cacggggccg
    10081 gaataagaat cgacaacctt gaccttgact ttgaactccg ccaatttttc ttcgatggtg
    10141 atgctgtttt ccaacagttc ttcttcggtt tgcgtcgcct cgggattgaa cagcggcggc
    10201 agaagcaggt cggttgtcgg caggtgttcg gataccgcac cggtttcttc agatgggaac
    10261 gccccttcat ccgcttccgt atccgatacc cggcaggacg ggcgttcaga cggcacattt
    10321 tcaaacggac aaaccgcctg actgtcatgc ccgaacgctt cggtttccga cagatattgc
    10381 ccgtttgacc gctctgccgc accttcactg ccgtcatctg caatagcggc ggtttcctcc
    10441 tgccaacctc cattcaaaac atcatcggca agatggtcgg tttcagcaat gcggctgcgt
    10501 tgcgcctgct cgaatcctgt cggcggctca taggtacggt tgtagatttc cgatacggga
    10561 ggcggcggcg gaatatcgat tgcgggcatg ggaactttcg gcacttcggg tggttcgacc
    10621 acccaagcat cgggcgcagc aggttcttca atatagatat cggcagcttc atcataaacc
    10681 ggaccgccgt atcccacaga cgaaacggtt tccgtgaaaa cattttcggt ttcagacggc
    10741 atttcgacat cccgtttccc gacaaccgtc cgactttccg gaatcaggcg cgaaatccgc
    10801 gcctccgcag aaaccgttgc cttgttcggt tctttcaaat tgacggaaac gcggcggcag
    10861 gcatcgcgga cggctgtgcc gtctgaaacg gactgtcctt gggaaacatc cggggatttt
    10921 gcctccgcct gccctgcatg acggcggaac ggcgcattcc gtgcattttc ggctttgact
    10981 gcagaaaagg cagacggatg ctgcttctca aaatacggct caaatccgta atccgcagac
    11041 tcggaaaacg cttctttgtc cgcatcgaac atacgggaat acgtgcgttg aagcacaggg
    11101 tcgtccaaac cgataatctg tagcccttcc atcggggtat cggacacgcg gactttgggg
    11161 accgctgttt cgtttttctc aaatgcatcg atatagcgtt ttttcgtttc ccttaaagcc
    11221 gcgctgttta aagcacgcgt tgcttcttcc aaagtgattt ccttaaaaac cggacggacg
    11281 ggcgaaattt cgctttcaga cggcatcaac ccttcagcaa tactccggtc gaatgggatg
    11341 cggcggttgt cgattacggc agttgcaatg tcttccgtat cggcagcctc ttcctccgca
    11401 gcttctgctt cttccgtttc cgcttcttcc gtcccgtttc cactgtctga atatccgtcg
    11461 gatgcggtcg gaacatccgc acggtttgca gaagcaggcg gaacgggcct gtccggttcg
    11521 gattcgtgcg ccagatagtt tcggcagaaa cggacgacac cgacaaacag ccaatacact
    11581 gccgttttga cggcatggaa aagcatcagg gcaagttcgg gaaaatcggg cataccgtct
    11641 ttgatttcag gcagctccgc ctgtttttcc cctttctttt cctgccacgc agaaacctcg
    11701 cgcatccact cgcgttcgga ttgtgcgcgg acaaaaaaca agccggcaag cgcaagcaac
    11761 aaaataacga tcaaaactat ccaaaacata tgccgtttcc cgcaagacaa acgattaaac
    11821 gggtttattt taacgtgttt ccatcccaaa aaaaacaacc ggtcggcaat tctgccgaaa
    11881 ccgattcggc tttcggtttg cccccgccga cagtataata gccgtccttt cccttaatct
    11941 ttgcggcatt gccatgattt tccaaacagt ctggttttca gatatggtac tgtcggtttc
    12001 gtggattgtc cttatcctga tactggcagc ttccgcgccg tccgcattcc gctcgcttgc
    12061 ccgctaccgt tccgcccttc ccctgtgtac cgtaattttt tccgcagcat ggtgtctgaa
    12121 cgcctctgcc ggcggcggac agcttgccca gatgaactac catctgctcg ccgtcaatct
    12181 ggttgcactg atggtgggca cttccgccgc cttttggctt gccgcgctgc tgatgctgcc
    12241 ctactgcctg ctgtttgccg gttctgccgg cgcatatccg ccgaacgctt tggtgctgat
    12301 tctgcccgcg cttgccgtca accgcctgtc gcgtatgctg gtcaaccgcc tgccgcccaa
    12361 tattttcatc tttattttcg tcaacggctt tctcgcttcc gccgccggca ttctgttgac
    12421 cgggctggtg ctgaccggta ttttggatgc tgcaaacgct tttccgtccg aaatattgtg
    12481 gacgaccgcc ctgcccgtct ttattctgct ggcgtgggcg gaagccttcc tcagcggcat
    12541 ttcaactgcc atatttgttg cactgaagcc ccattggatc aacactttcg acgacaaccg
    12601 ctatctgaag tccgaccgcg gcatatggcg gtagccttca gacggcatag gcagacaaaa
    12661 ccatgctttc caacatcatc cccctttcca tcggtgcggc actcggtgca acggcgcgtt
    12721 ggctgctcaa cctcgccgtt cctgccgcca tgtctcccgc cacgggcaac ctgtttgcca
    12781 actggacggg cgcgctgctc atcggcatct ttgccgaaac catcaatcac ccgcaatgga
    12841 agctgctgct gattaccggt tttctcggca gcctgaccac gctttccgga ttttcactgg
    12901 aaaccgtaac cctgctccaa tcgaatcgtc ctgcttcagc acttgccaat attttcctac
    12961 acacggcagg ttcgctgctg ctgacttggc tcgggctgaa gataggggcg gcagtcaaat
    13021 aaccgcaaca aattgcctcc ctttgttttg acataatcta taaattagct tcgcttgttt
    13081 aaatactggc tgtgc
//
LOCUS       DAXHMX010000048        13009 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHMX010000048 DAXHMX010000000
VERSION     DAXHMX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.048"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(57..677)
                     /gene="galE"
                     /locus_tag="ACFM4S_000979"
                     /pseudo
     CDS             complement(57..677)
                     /gene="galE"
                     /locus_tag="ACFM4S_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(756..1100)
                     /locus_tag="ACFM4S_000980"
     CDS             complement(756..1100)
                     /locus_tag="ACFM4S_000980"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC6050685.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQPPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(1093..1287)
                     /locus_tag="ACFM4S_000981"
     CDS             complement(1093..1287)
                     /locus_tag="ACFM4S_000981"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6050686.1"
                     /translation="MRKVFHYEQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNA
                     VPPLLAKAIAESILKMSENE"
     gene            complement(1265..2095)
                     /locus_tag="ACFM4S_000982"
     CDS             complement(1265..2095)
                     /locus_tag="ACFM4S_000982"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6050687.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKTSRLYYRRYAQSFSL
                     "
     gene            complement(2126..4399)
                     /locus_tag="ACFM4S_000983"
     CDS             complement(2126..4399)
                     /locus_tag="ACFM4S_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6050688.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDGLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPLDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQNQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            complement(4463..5113)
                     /locus_tag="ACFM4S_000984"
     CDS             complement(4463..5113)
                     /locus_tag="ACFM4S_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6050689.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5110..5907)
                     /locus_tag="ACFM4S_000985"
     CDS             complement(5110..5907)
                     /locus_tag="ACFM4S_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6050690.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5907..7070)
                     /gene="ctrB"
                     /locus_tag="ACFM4S_000986"
     CDS             complement(5907..7070)
                     /gene="ctrB"
                     /locus_tag="ACFM4S_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC6050691.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7085..8260)
                     /gene="ctrA"
                     /locus_tag="ACFM4S_000987"
     CDS             complement(7085..8260)
                     /gene="ctrA"
                     /locus_tag="ACFM4S_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC6050692.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8387..9520
                     /gene="neuC"
                     /locus_tag="ACFM4S_000988"
     CDS             8387..9520
                     /gene="neuC"
                     /locus_tag="ACFM4S_000988"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC6050693.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHLDLELHLIVTGMHMMKT
                     YGRTYKEATRENYQHTYLFSNQIQGEPMSAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            9524..10210
                     /gene="neuA"
                     /locus_tag="ACFM4S_000989"
     CDS             9524..10210
                     /gene="neuA"
                     /locus_tag="ACFM4S_000989"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC6050694.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10211..11260
                     /gene="siaC"
                     /locus_tag="ACFM4S_000990"
     CDS             10211..11260
                     /gene="siaC"
                     /locus_tag="ACFM4S_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HFC6050695.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11344..12831
                     /locus_tag="ACFM4S_000991"
     CDS             11344..12831
                     /locus_tag="ACFM4S_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HFC6050696.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDYFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
BASE COUNT         3768 a         3062 c         2647 g         3532 t
ORIGIN      
        1 ccgataaagc ccgcaccgcc ggtaacgagg atggtttttt tatttgcggt ttgcatttaa
       61 tccccgtatc cgtcggggtt gcggctgacc caacgccacg aatcttccat catttgtgca
      121 aggctgcgtt tggtttccca gcctgtctct tgtttggtat gggacgggtc ggcaaaggaa
      181 caagcgaggt cgccggcgcg tcggggttgg atttgatagg gaatatttaa gccggatgcg
      241 gcttcaaagg cacggatgat ttccaaaacg gaataggcgc gccccgaacc caagttgaac
      301 aaatgtacgc cggcaacgcc gccttttgcc tgcatagccg cgacatggcc ttctgccaaa
      361 tccatcacat gaatatagtc acgcatcccc gtgccgtcgg gggtagggta gtcatcgcca
      421 aataccgcca attgcggcag tttgcctgcc gccacttggc agatataagg caacaaatta
      481 ttcgggatgc cgtttggctg ctcgccaatc aaaccacttt cgtgcgcgcc aatcggattg
      541 aaataacgca acaaaatcac gctccagcgc ggatcggctt tttgaatatc agtgagaatg
      601 cgctcaacca ttgatttcga tgcgccataa ggattagcgg tatcgcccgg gcgcatatct
      661 tccgtatagg gcattttacg tatcatggcg cgacgcatta aagtaaacgc caaagcgcgt
      721 ttatactggt tatccagttt ccctatattt ttacgttaca aatcaagttg ttggtattca
      781 tcgtgaaaat agaatttttc ggcataacgc tgactcataa aaccggcatg cgggctgcct
      841 gaaaaaatca ttgcgacatc atcgtcattt aatgtttcat ggttgttttc tattaatgct
      901 aaagcaattt ggtaattaat tgccaaaata tcgttagtaa tcacacgata gccgcgtttt
      961 ttggcttcgt aggcaaagga acagcctcca ctaaatacat ctgcaactgt atctacatca
     1021 gacggaggct ggtcacaaat ccaggaagct attttctctt tattaccgat atagttaatt
     1081 ttcggatatt gcttattcat tttcactcat ttttaaaata ctttcagcaa tagcttttgc
     1141 caataaaggc ggtacggcgt taccaacctg ctgctgctgg gcaattttgc tgccgcaaaa
     1201 aataaaatta tcagggaaag attgtaaggc agctaattca cgaacggtta acgcccgatt
     1261 ctgttcatag tgaaaaactt tgcgcatatc tcctgtaata caaacggctg gttttgttgc
     1321 tgttgtaacg gatgtattta cggatatcac ctgttttcgg acgtaatggt tcaggaatat
     1381 cgttacggtt acctccattt ttaacaaatg ccattttttc taacatttgt gccgaatgat
     1441 tcatagcttc atgatttgca acgtgtggat tgctttcgcc agcagccagt tttggaaaat
     1501 gtcctattgc tgatccaaca gtctgatggg aaatctgcaa aggttcggga aaggaaattt
     1561 tgcctttatc cctcctcccg ataaatatca ctcggctacg tatctgagga acaccgaaat
     1621 cggctgcact cagtatctta cattccaccg aataaccgat attctgaaat gcttgaataa
     1681 tctcaatacg tgttttacct gaattgtgtg tatagagtcg cgctacattt tccataacaa
     1741 aaaaatatgg ttggacaatt ttaactattc ggacaaactc tttaaataaa tggttgcgtg
     1801 ggtcatctgt aaatgtccgt ccaatctttc ctgccatact aaaaccttga caaggtggtc
     1861 ctccaataat caaatcaact gcttgtccgt taagacaatt gattaaatct tgttcggtta
     1921 gtgtggttaa atctttttgc agtaattgat gatgggggaa gttggtacgg taagtctgac
     1981 aataatcaga ctccatttca acagaaagca attgttggaa tccggcttgt tcaaaaccca
     2041 aggataggcc tcctgctcct gagaaaaggt caatataggt aagtggtgtg tgcataagtc
     2101 aaaatccata aactcttcga ttattttacc gcttcagctt cgcaaacgca tccgccatcg
     2161 ccgaattggt cggggcgcgg tcgttgcgtt ggggtttgcg gccggctgtc cgttcgcggc
     2221 tgcggttttc agacggcatt ttatgttttg cgccgcccgg ttcgtcatcc aagcgcatgg
     2281 tcagcgcgat gcgtttgcgt gcagcatcga cttccagcac tttcactttc accacgtcgc
     2341 cagctttcac cacttcgcgc gggtcttgga cgaacttgtt ggacagggcg gagatgtgca
     2401 ccaagccgtc ctgatggacg ccgatgtcca cgaacgcgcc gaagttggcg acgttggaaa
     2461 ccacgccttc gagtatcata ccgacttgca agtcgctgat ttcgtggata ccttcggcaa
     2521 acgatgccgt ctgaaactcg ccgcgcggat cacggccggg tttttccagt tcggacagga
     2581 tgtccaaaat ggtcggcagg ccgaagcgtt cgtcggtgaa gtcggacgct ttgatttgct
     2641 tcacgcgctc gcggttgccg atgagttcgg cggcgctaat gccttgttgc gccagcattt
     2701 tggcgacgac gggataggct tcggggtgga cggcgctcgc gtccaacggc tctttaccgc
     2761 cgttaatccg caaaaagcct gccgcctgct cgaaggtttt ttcgcccaaa cgcggtactt
     2821 tcagcaattt tttgcggctg tcgaacgcgc cgttttcatc gcggtaggca acgatgtttt
     2881 gggcaagggt ttgattcaag ccggaaatcc gcgccaagag cggggcggag gcggtattca
     2941 cgtccacgcc gacggcgttc acgcagtctt cgaccactgc gtccagcgat ttggcgagct
     3001 ggttttggtt cacatcgtgc tgatactggc ccacgccgat ggatttaggg tcgattttga
     3061 ccaactcggc aagcgggtct tgcagcctgc gggcgatgga caccgcgccg cgcagggaaa
     3121 cgtccaagtc ggggaactcg cgcgccgcca gttcggacgc ggaataaatc gacgcgccgg
     3181 cttcggacac gacgatttta tgcagcccca ttttcgacat ttcacgcacc agttcgcccg
     3241 cgattttgtc ggtttcacgg ctggcggtgc cgttaccgat ggcgatgagc ttcacgccgt
     3301 gctgtttaat caggcgcgac agcgttgcca acatattgtt ttcttgatgc aaatagacga
     3361 tgacggtatc cagcagcttg ccggtgtcgt ccaccacggc gcatttcacg ccgttgcggt
     3421 agccggggtc gagacccagc gtggtcagcc gtccggcggg cgcagcaagc agcaagtctt
     3481 tgagattgcg ggcgaacacg gtaatcgcgt cggtgtcggc ggcttctttc aaacgattga
     3541 gagcttcaag ttccaacgac aaaaagattt tcgcgcgcca agtcaggcgc acggtgtcgc
     3601 gcagccattt gtggccgtct gaaactttga agcggcgggc gatgatttgc tcgtattcgc
     3661 tttgctgcgt aatcggcgtg tcgtcgggct gatatttgag cgcgatgttc aacacgcctt
     3721 cgttgcgtcc gcgcaaaacc gctagcgcac gatggctggg catcgcacga ataggttcgc
     3781 ggtggtcgaa ataatcgctg aatttttcgc cttcggtttc tttgcctcca acaacttgcg
     3841 catggatttc ggcttcgttc cacaacttat cacgcagcgt gccgatgagt tccgcgtctt
     3901 cggcaaactg ctccatcaga atcgcacgcg caccgtccaa cgcggctttg gcatcgggga
     3961 cgttttcgtt caggtagcct tgcgcggcag cttccacgtc cagcggctgc tcggcaagca
     4021 acacgtccgc cagcggctgc aaaccgtgtt cgcgcgcgat ttgcgctttg gtgcggcgtt
     4081 tgggtttgta gggcagatac aggtcttcca gcgcggtttt gttatcggcg gcttcgattt
     4141 gcgcgcggag gccgtctgaa agcttgcctt gctcttcaat gctttttaaa acaacggctt
     4201 tgcgctcttc caactcgcgc agatattgca gccgctcggc aagccggcgc aactgcgtat
     4261 cgtccaaccc gcccgtcgct tccttgcggt agcgggcgat aaacggcacg gtcgcgccgt
     4321 cgtccaaaag ctcgacggcg gcggtgattt gcgcggcagt cgcggagagt tcttgggaaa
     4381 gaatttgagt aatgttcatc aatagaattc caacggacag gccgtctgaa atttcagacg
     4441 gcctgattta aaaacaatcg ctttaaggca gcgaattata atattcgtag gctttgtcca
     4501 tatcttcaaa ctggtacata tgcccttttt ccagcaccat cgcattatcg caatattgct
     4561 tcatggcgct gtggctgtgc gacaccaaga tgatggaacg gtctttgcgc ttttcaaaca
     4621 actcgtactt acatttatcg gcaaaacgcg agtcaccaac tgcaatcact tcgtcaatca
     4681 aatagcaatc aaactctacc gccaacgaca aagcaaatgc caaacgcgcc ttcatacctg
     4741 aagaataacg cttgaccggc tcgtaaagat attgccccaa ctcggaaaac tcttcggtaa
     4801 agttcttcac ataatcgata tcgacattgt aaatccggca gatgaaacgc aaattgtcca
     4861 taccggtcag actgccttga aacgcaccgg agaatgccaa aggccaagaa atactcattg
     4921 tccgcttgat ttcacccgtg gtcggcggct caacgccact gatcaaacgg atgagcgtcg
     4981 atttacctgc accgttgcgg ccgagaatac cgattttctc gcccttctcc attttgaagc
     5041 taatatcgtg caagactgtc cgccaacctt ggcgggtcag atagcgtttg gaaacgtgtt
     5101 caactgaaat cattgcggct cgactccttt actgaattta ctgaccatcg ccaagccaaa
     5161 caacaacaac accagattgc acaatacgat ataccaagga ttttcatagg taattacatc
     5221 gctgccaaaa tatccggcac ggaacatttc tgtgccatgc accatcggga tcatcaatgc
     5281 atactcttgc actttcggag gcaaattatg tacaaaaaag aatgcacccg ataacggcat
     5341 catcacaaaa gtcaatgtgc cccaaatctt gccaaacggc tcgaaattaa aggcaatcga
     5401 acaaatcacc aaacccaaac caatcgcaaa aaaagccatc aaaagccaag ccatcagcat
     5461 ataaaacata tctgccggca tttcaatcca gccaatcgca atcaataccg ccataatcac
     5521 aatctgcgca atggttgcac cagcaatttc caaaatcatg cgcgccaaga tggtatccaa
     5581 aactcttaca ttgcggtgat aaagcaagct ggcatttgaa gaaatcgacc caactgcccg
     5641 ttttgaggca ttacgccaca tcatcaacat cggatagcca gtaatcgcaa atgcgacaat
     5701 attcaaagtt gaatatcggt ctgcccttaa aaatttccac atcaagacga taacgaatgt
     5761 catcagcaac ggctcaacaa acagccataa aaagccaata ttattgcgac cgtaacgggt
     5821 gataatttcc cgcatcaaaa gcgcaccgat tacgcgcctt tgaatggcta aagattccca
     5881 aaatgatgtt ttatgcaagg ctttcatcag tttttatgct cacgaatgct ggcagtcaac
     5941 aggctcaaaa taccataaac catcaagccg ataatcagag tggcaacaat gttgtataac
     6001 cgtttaggct catgtgccaa atccggcagg ctcggttgcg agatcacttc caaataaagc
     6061 tgctgacggt ctgcttcaac cttggcactt tccaaagaag tcatggcggc tgccaactgc
     6121 tgctctgcca actggttttc caaatacaca cgctgatact cggcagcctg attagacaac
     6181 gaagaatgcc caccgcccga aatggcacgt aactgttggt caatttcttt acgcaagctc
     6241 tgctcacgcg cctgcaaacc cggaatctgc ggattctccg gagtgactgc tttcacctga
     6301 tccagctggg tttgaatcac aatcaattca tcttgcagct tggaaaccaa ccccatttgc
     6361 acttccgatt gcgctttcaa atcaaaaacg ccattggcaa tccggtaatc cgtcagattc
     6421 tgagaggctt cctttacccg ctctgccgcc gttttcacta cttcttccgc atagcgcacc
     6481 gtatcagcac gtgcacgatc gttcaactgg ttaatcaatg cttcaccttg ttttaacaaa
     6541 gcctcattga ttttcttaga ttccagcgca tcaaaggaag ttacattcaa cgtggaaata
     6601 cccgaaaccg tatcaaaatt gatcatcacc tgatttttat agtattgata aaaagcctct
     6661 tcctcgccac ggaacccaaa cccattaaag cggctgaacg catcaccttt ggtttcataa
     6721 aactcacgca ccggcaagat tttacgcagt tcatccaaag acgagcgcga acgcatatac
     6781 tccccaaccg tgtaaatatc atcttgcgca cgggcaaaac ctgtgccctg caaaatggca
     6841 cccaggccat tgagagaaga ttggctttta ggcgagcgca ccacaaagct cgattgcgac
     6901 gtaaaacgat cggaagcgaa gaagccgaaa tacaccaacg aaattaccgt agggataatc
     6961 accgttaccc aaaataaagg gcttagcttt ttaatccaac ttttcttttt cggcttttta
     7021 cgctcggctt tggtttcagt ggcaactgcc acaggaagtt gctcagacat cttaattact
     7081 cacattaatt agttaaatta ttaatactgt tcgcgccact ggtaaccggc gagaacacaa
     7141 acgacaagaa tttctgcact tcagccaacg gcgcattcga cacatacaat acatctttat
     7201 tcttcacagg aaagcgctgc atagaaaata gcgaatgcgc atcagccata ttcacacgat
     7261 ataccgttgg aatctctgcc tcactgccat aaccttgagc aatccattta tcctgacgtt
     7321 ctgccggcaa ttccaccaat ggcgtatagc ggaacacaaa cacaccacgc gcatcagaac
     7381 ggcgatcttg caaaccgccc atacggccaa tggcttcaga aagcgataag cctctggctg
     7441 aaaaaccgat ttcttgtgtt ctccccaccg cacccataga cgtaaaggta tagggattgg
     7501 taatcatggt aaccacatca ccgcgacgca gcaaaatatt ttgtcgcgga tttgcaacta
     7561 aatcttccaa ggcaacagtt cgtactacat tgccacgtgt cagctgcaca ttcgtatcct
     7621 gcacatttgc cgttgaacca cctaccgcag ccaccgcatc caacacacgc tcaccggctg
     7681 ccgtcagcgg catacgcaca ctattcccag cacgaatcac cgacacattc gccgcattat
     7741 tctgcaccaa acgcaccatc acttgtggct gattggccat ttttttcagg cggcctttaa
     7801 taatttcctg aacctgacca ggcgttttac cgaccaccga aatatcgcca acaaacggca
     7861 cagaaaccgt accacgtgcc gtgaccaact gctctggcaa cttagtttga tgcgcactac
     7921 ccgagcccat cgaagaaagg ccaccaccaa acaatactgc cggcggcgct tcccaaatca
     7981 taatatccaa tacatcacca atatttagcg taccagccga agcataacca tcgccaaact
     8041 gagtgaatga ctgatttatc tgagccttat ataataactg agcaaccgta tgattcacat
     8101 caatcagctc cacttcagga atttgaactt cagattgttg ccctaaagag acaatttttt
     8161 ttgcgctggg gcctgatgaa ggaatcgcag agcatcctac aattaaactt ccacacaata
     8221 ataatactgc gtgacgaata taaaatttca ctttaaacac aagccaaatc ctaatataat
     8281 tataaatggc ctaattatag cacttaatcg aaataaattt atgagtacgt agagtataat
     8341 tagtattctt ctttccaact tccttatgct tatagattct aaaatcatga aaagaattct
     8401 ttgcattaca ggtactagag ccgacttcgg caagctaaaa cctttattag cctatattga
     8461 aaatcaccta gaccttgaat tgcatttgat tgtaactggt atgcatatga tgaaaacata
     8521 tggcagaacc tacaaggaag caactcgaga aaactatcaa catacatatc tgttttcaaa
     8581 tcaaatccaa ggtgaaccaa tgagtgccgt tttaggcaat accattacgt ttatctctcg
     8641 tctatctgat gaaattgaac ctgatatggt catgattcac ggcgaccgtt tagaagcact
     8701 agcaggcgca actgtaggtg cattaagcag ccgtttagtt tgccatatcg aaggtggtga
     8761 actatctggt acagtagatg actccattcg tcattctatt agtaaacttt ctcatatcca
     8821 cttggtagca aatgaacaag ctgtcactcg cctagtgcaa atgggagaaa aaagaaagca
     8881 tattcacatc atcggctccc ccgatttaga tgttatggcc tcttccaccc tcccatcctt
     8941 agaagaagtc aaagaatatt acggtttacc atacgaaaat tatggtattt ctatgtttca
     9001 ccccgtgact acagaagcac atttaatgcc acaatatgcg gcccaatatt tcaaagcatt
     9061 agaattaagt ggccaaaata tcattagcat ctaccctaat aatgacactg gcactgaaag
     9121 tattctgcaa gaattattaa aatatcaatc tgataaattt attgcatttc cttccattcg
     9181 atttgaatat tttttagtct tattgaaaca tgctaaattt atggtcggca actcaagtgc
     9241 aggtattaga gaagctcctc tctacggtgt cccttcaatt gatgttggta cacgccaaag
     9301 taaccgccat atgggaaaat ctattattca tacagattat gaaactaaaa atatctttga
     9361 tgcgattcaa caagcatgca gtttaggcaa atttgaagca gatgatacct ttaatggcgg
     9421 agatactcgc accagcacag aaagatttgc tgaagtaatc aacaatcctg aaacgtggaa
     9481 tgtttctgct caaaaacgtt ttatcgattt gaatctttaa attatggaaa aacaaaatat
     9541 tgcggttata cttgcgcgcc aaaactccaa aggattgcca ttaaaaaatc tccggaaaat
     9601 gaatggcata tcattacttg gtcatacaat taatgctgct atatcatcaa agtgttttga
     9661 ccgcataatt gtttcgactg atggcgggtt aattgcagaa gaagctaaaa atttcggtgt
     9721 cgaagtcgtc ctacgccctg cagagctggc ctccgataca gccagctcta tttcaggtgt
     9781 aatacatgct ttagaaacaa ttggcagtaa ttccggcaca gtaaccctat tacaaccaac
     9841 cagtccatta cgcacagggg ctcatattcg tgaagctttt tctctatttg atgagaaaat
     9901 aaaaggatcc gttgtctctg catgcccaat ggagcatcat ccactaaaaa ccctgcttca
     9961 aatcaataat ggcgaatatg cccccatgcg ccatctaagc gatttggagc agcctcgcca
    10021 acaattacct caggcattta ggcctaatgg tgcaatttac attaatgata ctgcttcact
    10081 aattgcaaat aattgttttt ttatcgcccc aaccaaactt tatattatgt ctcatcaaga
    10141 ctctatcgat attgatactg agcttgattt acaacaggca gaaaacattc ttaatcacaa
    10201 ggaaagctaa atgcaaaaca acaacgaatt taaaattggt aatcgttcag taggttacaa
    10261 ccacgaacca ttgattatct gtgaaatcgg catcaatcat gaaggctctt taaaaacagc
    10321 ttttgaaatg gttgatgctg cctataatgc aggcgctgaa gttgttaaac atcaaacaca
    10381 catcgttgaa gacgaaatgt ctgatgaggc caaacaagtc attccaggca atgcagatgt
    10441 ctctatttat gaaattatgg aacgttgcgc cctgaatgaa gaagatgaga ttaaattaaa
    10501 agaatacgta gagagtaagg gtatgatttt tatcagtact cctttctctc gtgcagctgc
    10561 tttacgatta caacgtatgg atattccagc atataaaatc ggctctggcg aatgtaataa
    10621 ctacccatta attaaactgg tggcctcttt tggtaagcct attattctct ctaccggcat
    10681 gaattctatt gaaagcatca aaaagtcggt agaaattatt cgagaagcag gggtacctta
    10741 tgctttgctt cactgtacca acatctaccc aaccccttac gaagatgttc gattgggtgg
    10801 tatgaacgat ttatctgaag cctttccaga cgcaatcatt ggcctgtctg accatacctt
    10861 agataactat gcttgcttag gagcagtagc tttaggcggt tcgattttag agcgtcactt
    10921 tactgaccgc atggatcgcc caggtccgga tattgtatgc tctatgaatc cggatacttt
    10981 taaagagctc aagcaaggcg ctcatgcttt aaaattggca cgcggcggca aaaaagacac
    11041 gattatcgcg ggagaaaagc caactaaaga tttcgccttt gcatctgtcg tagcagataa
    11101 agacattaaa aaaggagaac tgttgtccgg agataaccta tgggttaaac gcccaggcaa
    11161 tggagacttc agcgtcaacg aatatgaaac attatttggt aaggtcgctg cttgcaatat
    11221 tcgcaaaggt gctcaaatca aaaaaactga tattgaataa tgcttattaa cttagttact
    11281 ttattaacag aggattggct attacatata gctaattctc attaattttt aagagataca
    11341 ataatgctaa agaaaataaa aaaagctctt tttcagccta aaaagttttt tcaagattca
    11401 atgtggttga caacatctcc attttatctt acccccccac gtaacaattt atttgtcata
    11461 tctaatttag gtcagcttaa ccaagtccaa agcctaatta aaatacaaaa attaaccaat
    11521 aatttactag taattttata tacttctaaa aacttaaaaa tgcctaagtt agttcatcaa
    11581 tcagctaaca agaatctatt tgaatctatt tatctatttg agcttcctag aagccctaat
    11641 aatataactc ctaaaaaatt actttatatt tatagaagtt acaaaaaaat ccttaatatt
    11701 atacagcctg ctcatctcta tatgctgtct tttacaggcc actactccta tctgattagt
    11761 attgcaaaaa aaaagaatat tacgactcat ttaattgatg aagggactgg aacatatgct
    11821 cctttattag aatcattttc atatcatcca acaaaattag aacgttattt gattggaaat
    11881 aatcttaata ttaaaggata tatagattat tttgacatat tgcatgtccc ctttcctgaa
    11941 tatgctaaaa aaatatttaa tgcaaaaaaa tataaccggt tttttgcgca tgctggagga
    12001 ataagcatta ataataacat tgcaaactta cagaaaaaat atcaaatatc taaaaatgac
    12061 tatatttttg ttagtcaacg ctaccccatt tcagatgatt tgtattataa gagtatagta
    12121 gaaatcttaa acagcataag tttacaaatt aaaggaaaga tatttattaa actacaccca
    12181 aaagagatgg gcaacaacta tgtaatgtct ttatttctaa atatggtaga aataaaccct
    12241 cggctggtag ttattaatga acctcctttt ctaattgagc ccctaatata cttaacaaat
    12301 cctaaaggaa ttataggcct ggcctctagt tctttaattt atacaccatt actctcaccc
    12361 tcaacccaat gtctttctat tggagagtta attattaact taattcaaaa atattcaatg
    12421 gtggaaaaca ctgaaatgat ccaagaacac ttagagatta ttaagaaatt taattttatt
    12481 aatatactaa atgatttaaa tggggtaata agtaaccctc tctttaaaac agaagaaaca
    12541 tttgaaacac ttcttaaatc tgcagaattc gcatataaat ctaaaaacta ctttcaggct
    12601 attttttact ggcaacttgc cagcaaaaac aatattacct tattagggca taaagcatta
    12661 tggtactaca atgcacttta taatgtaaaa caaatttata agatggaata ttcagatatt
    12721 ttttatatcg ataatatctc cgtagacttt catagtaaag ataaattgac atgggaaaaa
    12781 attaaacatt attactattc cgccgacaat agaattggta gagatagata aaaatactat
    12841 gacttctgat ctccatacgt atgcttgttt tcaaaaagca gcattaagat agataagtga
    12901 agcttgatga gagccatttt tgtggtcatc gccaaggtac acgcggagag taatcgacaa
    12961 cagagctgct gtattctggc ttttaaagcc cagtagtaaa gtttataag
//
LOCUS       DAXHMX010000049        12813 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHMX010000049 DAXHMX010000000
VERSION     DAXHMX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.049"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            390..1106
                     /locus_tag="ACFM4S_000992"
     CDS             390..1106
                     /locus_tag="ACFM4S_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC6050697.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1209..1634
                     /locus_tag="ACFM4S_000993"
     CDS             1209..1634
                     /locus_tag="ACFM4S_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050698.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1878..2078
                     /locus_tag="ACFM4S_000994"
     CDS             1878..2078
                     /locus_tag="ACFM4S_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050699.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2146..2505
                     /locus_tag="ACFM4S_000995"
     CDS             2146..2505
                     /locus_tag="ACFM4S_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050700.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVSNTSSTLKMVRSN"
     gene            2530..3384
                     /locus_tag="ACFM4S_000996"
     CDS             2530..3384
                     /locus_tag="ACFM4S_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFC6050701.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTPLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3454..3669
                     /locus_tag="ACFM4S_000997"
     CDS             3454..3669
                     /locus_tag="ACFM4S_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050702.1"
                     /translation="MTITEEYSIDIRVTSEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLTKDNLKNAA"
     gene            3704..3964
                     /locus_tag="ACFM4S_000998"
     CDS             3704..3964
                     /locus_tag="ACFM4S_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO1622 family putative holin"
                     /protein_id="HFC6050703.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3961..4254
                     /locus_tag="ACFM4S_000999"
     CDS             3961..4254
                     /locus_tag="ACFM4S_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050704.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4257..4448
                     /locus_tag="ACFM4S_001000"
     CDS             4257..4448
                     /locus_tag="ACFM4S_001000"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HFC6050705.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4448..4588
                     /locus_tag="ACFM4S_001001"
     CDS             4448..4588
                     /locus_tag="ACFM4S_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HFC6050706.1"
                     /translation="MDTLLKIIIALSFSGAEALVVWLFITAADAVLRRFQKPSHYRRV
                     KK"
     gene            4585..4794
                     /locus_tag="ACFM4S_001002"
     CDS             4585..4794
                     /locus_tag="ACFM4S_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HFC6050707.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(4852..5769)
                     /locus_tag="ACFM4S_001003"
     CDS             complement(4852..5769)
                     /locus_tag="ACFM4S_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFC6050708.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(6638..6994)
                     /locus_tag="ACFM4S_001004"
     CDS             complement(6638..6994)
                     /locus_tag="ACFM4S_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC6050709.1"
                     /translation="MAERNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAI
                     LTVLNQREFVCAGVLGGALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQE
                     FEAVARKWKKKPLKTK"
     gene            complement(7542..7922)
                     /locus_tag="ACFM4S_001005"
     CDS             complement(7542..7922)
                     /locus_tag="ACFM4S_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6050710.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(8079..9275)
                     /locus_tag="ACFM4S_001006"
     CDS             complement(8079..9275)
                     /locus_tag="ACFM4S_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC6050711.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVTR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRSLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(9466..9555)
                     /locus_tag="ACFM4S_001007"
     tRNA            complement(9466..9555)
                     /locus_tag="ACFM4S_001007"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9519..9521),aa:Ser,seq:cga)
     gene            complement(9659..9749)
                     /locus_tag="ACFM4S_001008"
     tRNA            complement(9659..9749)
                     /locus_tag="ACFM4S_001008"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9713..9715),aa:Ser,seq:tga)
     gene            complement(9832..10611)
                     /gene="yaaA"
                     /locus_tag="ACFM4S_001009"
     CDS             complement(9832..10611)
                     /gene="yaaA"
                     /locus_tag="ACFM4S_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6050712.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AVEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(10775..11962)
                     /gene="dapC"
                     /locus_tag="ACFM4S_001010"
     CDS             complement(10775..11962)
                     /gene="dapC"
                     /locus_tag="ACFM4S_001010"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6050713.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLDPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            complement(12039..12491)
                     /gene="dut"
                     /locus_tag="ACFM4S_001011"
     CDS             complement(12039..12491)
                     /gene="dut"
                     /locus_tag="ACFM4S_001011"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6050714.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFAVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
BASE COUNT         3507 a         3294 c         2931 g         3081 t
ORIGIN      
        1 aatattaaaa attatcgtca tcaatgcggc ttccccagcc cgcggcgagc tttccacgac
       61 aggctttttt tatttgggtt ggtcgtctgg tttctttgag ttcaaaaaca ggtcgggata
      121 cttaagtttt attcgtgcag gtatcccgcg cttcgtccaa ttgaaaacgc attgagggct
      181 attccctgtt attcgaccaa cttccgcgta actgccgatt gattgcaaca ggcgtttgtc
      241 ttcattgact cttttatcca taaataaacc aaatgtttaa atctaatgct aatattaaac
      301 actatgttta gataaaaatc aagtcttgtg taacaacatt ttgtttaaat atgggagaat
      361 aattaagcca accgcgaata agattaaaaa tgacaatgca cgagacaact gacagacttt
      421 ttgagatagc caaagagcag ggagttttaa agccggctga catagcagag cgtctgaata
      481 tcagccaaca ggctttgaaa aactgggaaa gtcgtggcat agcggcaaag gcgctgcctg
      541 aagtagcaaa agcattcggt gtatctgaaa catggctgag aacaggtgaa ggcagccgaa
      601 ccgcgcccgt ccttattgac cccgacctac cccacgaagt caaagacatc caccgcccga
      661 tgacgtggag cagcaacgac ccgctgcccg acgatgatta tgttttcgtc ccctacctca
      721 aagagagctg cttcaaaggc ggagtaggca cgtatgaaat ccccgattac aacggctacc
      781 gcctgccgtt cggcaaatcc acgcttaaac gcaaaggcat caatcccgac aacgtgtttt
      841 gctgcaccct gaccggcgac agtatggagg aaaaaatcgc agaagacgcg gcaattgccg
      901 tagatacggg cgaaaccgcc atacgcgacg gcaaaatcta cgccttcgcc caggacggta
      961 tgttccgcgt gaagtacctg atacggcagc ctggcaacag cgttctgata cgcagccaca
     1021 acagcggttt ctatggcgac gaaaacgccc ccttggacag cctgaccgtt atcggcaggg
     1081 tattttggtg gagcgtgctg gattgaaaaa tgttgctatt acgacgagat aatattgata
     1141 gggctttcaa gattgtcaaa aataggcgtt ttgattcccc ttggtggcct ggtgagtacg
     1201 atgccggtat gaatttcctt ggcgtacaag gagagttgaa ggttcacgag ctgcatcaca
     1261 gaacagcaac cctgtgcttt gagtggcttg gcgaagtatc tgcgccgcgc agaaaagaga
     1321 actacaaaga cctcaagcca aatgtattgt atgactttga tggttccggt aagcattttg
     1381 ccaaccctga cgcaaggtat ctattgcccg tcggttcaag cggcttaatc ttaaaacata
     1441 tccaaatcga tgacgaagac actctgttgc ggctatggtg cgcacgaaat atcccaatgc
     1501 cacaccggct atctaaaatc ccgatgcttc ggcagtatta tctaagcaag gcatggcacg
     1561 aaatttatgc tatcaaccaa cacctaagga aaacgaagct gatagttgat gtggcatacg
     1621 accccacaga ttaaacaacc cgcccgcatt atgcgggctt ttttcatgcc ccgccgaacc
     1681 tgaaaacaac acaaaaccga caaagccgcc ttaataggcg gtttttttgc gcctgcgaaa
     1741 tctcaaaaat aaattctttt aataatcaaa atgtttataa accttaaaca aaaatcttaa
     1801 acagattgtt gcatattatt aaacgctatg tttaaataca cccatcgaaa caaacaacag
     1861 ccacatggag taccgacatg gaaaccatca ccatccctaa agaaaatttc gacgctttgc
     1921 taaatctcgc aagaaccgcc gtcgccctga acgaaaattt tattccaaaa ataaattacg
     1981 gagcatccac cataccagca gccggctttg ccgcacttaa cgagtttgga atagcccgct
     2041 ccaccgtgga atctttggaa attctcaaaa ttaaataacc tcctcggtat tgtcatcccg
     2101 tgataccggc aacctaacta acctctcttt aaaggaaaat caaaaatgga aacccaagaa
     2161 aagaaatttg ttcctgcctc caagcaaata ccgaccgtaa atagcggctg gttagaccgt
     2221 gaattaggcg aagccatgag tgaagcggtg cgtgcctgcc ttgctcacgg taaacagtca
     2281 gaaattactg tcaagctcaa aatccaaccc caaaacatcc aaagcggaac ggtaaaaatc
     2341 agccacgatg tagcaaccaa actgcccaaa gaaaaacgcg aaggcggcat cgtctttgct
     2401 acacctgacg gcaacatcca agccgacgat ccggcacaag gaaaattgaa actcaaacaa
     2461 gtttccaata cgtcaagcac gttgaaaatg gtcagatcca actaactcaa acacaaaagg
     2521 aaatctaaaa tggaaaccca agaaaacatg attaaaaccg ccttacaagc agctcaaaaa
     2581 cctttttttg agtttgcacc gaacaatact ccgcttgtat tcacaccaga ccaagacggt
     2641 ggctggagat acaaatcaca ccccgaattg atgcaaaacc cgtaccgcaa gtgcggcaaa
     2701 ttcctcatgc acgataccgc cagtcttatt aagtttgtac aaaaacacaa acaggacggc
     2761 acacaaatct acattgatgc cgatttcaaa tcagggcgta ttgatgtcac cgccgtcatc
     2821 aatggccata cgcaacaagc tccgggctgg ctcgattttt atgccgtata caccccgcaa
     2881 cacactacat cggcatccaa ctggctgaac aacaatgcgc accgcatgaa tcagatggag
     2941 ttttcccatt tcctgaccaa caatgcccgc aacatcgtat ccaaaaatcc cggtaacgaa
     3001 aattccgttt acccgaccgc cgccgaagta ttggatttcg cgctcaatct cgagtacacc
     3061 gaaaaaacca ccttcaaaca aggctatcgt gaacaggacg gcagaatcaa ctttaccttc
     3121 caatccgaag actcagggca aacagagaaa aacctcaaaa tgtttgaacg tttcggcatc
     3181 gagttcaccc cgcatcaagg cggtgcatcc tactttgttg aagccctgtt gaaattccgc
     3241 atcgacaaaa acagcggcgc actcgtcctg tggtacgaat tgcagcaaat cgatgctgta
     3301 atcgaacaag cggcaaaaga catttccgaa gccgtacaaa aagccttccc tgaaatcgac
     3361 atctatttcg gcgtaatcgc ctaaaaaaaa gtcgtctgaa atcgtgaacc ttcagacgac
     3421 ctccacaaaa acccaaatcc gaaagacccc aaaatgacca tcacagaaga atactccatc
     3481 gacatccgag taacctcaga acaggggaaa aacgactacg gctaccccac cgaacgctac
     3541 ggatgcgaca tcatcaacac agacggggag ctacttgtcg gcatagagcc agagtacaaa
     3601 accccctttg ccgccgtcag aaaggcgctc atctgtctga caaaagacaa cctcaaaaac
     3661 gctgcctgat attaagccgt taccccaata atgaggaatc aaaatgaaat acttgatccg
     3721 caccgcctta ctcgcagtcg cagccgccgg catctacgcc tgccaaccgc aatccgaagc
     3781 cgcagtgcaa gtcaaggctg aaaacagcct gaccgctatg cgcttagccg tcgccgacaa
     3841 acaggcagag attgacgggt tgaacgccca aatcgacgcc gaaatcagac aacgcgaagc
     3901 cgaagaattg aaagactacc gatggataca cggcgacgcg gaagtgccgg agctggaaaa
     3961 atgaactttc aggaagaaaa cgagaacatc aagaatgaca tagagattaa tctcttgcat
     4021 tccgctgaag ccgacagcgt ggaggcaatg atggacttgg cggtttacgg gctggcggcg
     4081 atggttgcca aacacaacaa aacgcgcggc gagcagcttg tgttacaggc gattgccggt
     4141 catatgcgga agcttttggc cgcgcttccc gaaaccgagt cattggtgca gaccgggaag
     4201 gtttaccgcc gacttgaaga tttgattttt aaaacgtatt tgccggagca gtaaagatga
     4261 acacgaatca agatacgatt ccatttggcg gcaaccttgt gatttgctgt tcgacgggaa
     4321 acggcgggga cgggcggttt tcttgtctga ttaccgatca gataccggat ttaaatcttg
     4381 tcagaagcgg gcaggcgttc ccgatgttca tttatgagta cgaaactaga gaaaaaagga
     4441 aagcctgatg gacaccctat taaaaatcat catcgcgctg tcattttccg gcgctgaggc
     4501 attggtggta tggcttttca tcacggccgc cgatgccgta ttacgccgct tccagaaacc
     4561 gagtcattac aggagagtaa aaaaatgaac atcaacgaac tgggcgcaag gatagaccgc
     4621 ccgaccatcc gcgaactgat tgcctacgca acctgccgca accgccccat ttcaaactcg
     4681 acactgctgc gtatggaaaa agacgggcgg ataccgtgcc gtctgaaaac cccactcaca
     4741 tcccccgtat gggacacccg cgaagttttg gaagccctgg ggttacagca ataaaaaaac
     4801 gcccgattag ggcgttttca cattcagacg gcttttgtat tccctactgc atcaaaccga
     4861 cgacaggttg cggatttcgg gcagcatcgg gcggattttt gccgcgtgtt ccgcgtcggc
     4921 gtgtgcgttt aaggcttcga gggcgtttgc ggcggctttg aggcggctgc gtgtttccgc
     4981 ccagaccgtc cacatcgtta ccgcctgttt gcagccgagc tgcttcagcg gcagggaaac
     5041 gtctctgccc atttggatgg cccacgcgcc gtatctgacg gcaacggcga ggtcgtacag
     5101 ggcgttgccg ctgatgggca gggcaggttg cggtgcgggc aatggttcgc ggtcgaggac
     5161 ttcgccgtac agcacgccgt tttcaactgc cacgcggccc atcacgctta aaacttcggg
     5221 gaactgttcg gcaggtactt ctttgtagct gcatccgaac ttgcttttga cggcagacca
     5281 aagggtaatg gcgatacgcg cctgcgcttc tttgggtgcg gatttggtca gggcgttgtg
     5341 cagttttttg acggcttcga tttgttcgcc ggtcaagccg tccggaaggg cgcgggtggg
     5401 tttgggggta actgttttta tgccgtctga aattttgccg ttaagtaaaa tttcaatctg
     5461 ctcatcgcac caaaccgcaa atttcggatt aagccaacgg gcaaagtgaa tagcgagttt
     5521 gggatgcagc catgtgcctt gctcactgcc accacgcttc acgataacta tttgatttgc
     5581 ttccgttagg atttttctcc taacgcttaa attctcagca agtgcagaga tatattgttg
     5641 agtttgttca ctttttaggt agtctttagg taacttgcca aagtgagatg caatggcggt
     5701 tgcatttaaa aaaccgtctt gacggaaaga tacaggggtg ttaccaaaat tgagaacaga
     5761 tacgttcatg atagtttcct agtttagttt cgaaagaccc aaatgggtgg tcgggaggtt
     5821 cgaaaaccta ctagaaaagg ccggcgttat tccccttgcg ggtattgtat tccgcaccct
     5881 cccgaccata ggaaactagt tcggatatat cgaaacaaac aaccaaacaa gaaaacttat
     5941 ggacgtaaaa aattcacgct gacggggtaa atgccgttct agtagaggtt ttcgacgcct
     6001 cgtgtttcgt aatatatcca atctttctat tttctgcaag cataaaaaag ccgccatgtg
     6061 gcggataggg tcgcggtctt caacagcatg taggatttag gaatgcccaa attgggcgca
     6121 ggggtgttga aaacacgcaa acaggcgcgc cgccagcctt acgggtaggc gcacccccac
     6181 ataggagtaa atcgatttga tacgtataga cgtaaaaaat tccactctat cgtgttggat
     6241 atacgctgtt tgtcaggtgt tttcaagcac cgtgggaaat attatataga gttatggaaa
     6301 tgtgtcaaga gaattagccc aaatgggcgg acgcgcggtt aagaacccac ataggagggc
     6361 ggacttattc ccctttcggg tcttgtattc gtcgcccacg cggtcataga aacttcctgc
     6421 tatcgaaaca aacaacaagg aaagaaaact atagacatga aaaaatcaca ttgacggagt
     6481 gattgccgct gtagtgtggt ttctgacgcc acgaacagga atataaaaca aaaccccctg
     6541 cacatgcaag gggtttccca aaaaaccgta ggcggcaaac tgaaaggccg tctgaatttc
     6601 agacggccta tgttgcggcg gatgttaagc tattaagcta tttggttttc agaggctttt
     6661 tcttccattt tcgggctacg gcttcaaatt cttgaaaggc cgtcggtgcg ctcctgatac
     6721 ggcgtatttc aaaaataaag ctgcttaggt tgtcccagtc acgtaacagc atggagtatt
     6781 cgaaatcttt atacattttc tcgtgcagtg ctccgcctaa tacgcccgca cagacaaatt
     6841 cgcgctggtt taaaactgtc agtatggctt cacggtcttt cttcttgtcc ggggtatccg
     6901 atgtataggt ggcgagtatg catccgtctg tttttgccag cccgtttact atggttatgg
     6961 cttcttgaag ggcggcattg ttacgttcgg ccatgatcag cgcgcttttt ggcgttttct
     7021 gtattcctca tgatgccgta agcggcaaca aaaacgccga taacagtcaa tatcggcgtt
     7081 gctatctgta ttaggttgtc agtcattgcc gtcccagccg tctgaaaggc ggaaatcacg
     7141 gctttccgaa taaatatgtc ccattgtcat ccccttaagc tgatgttgaa aataaatcct
     7201 tgcgtcggaa tatacggcat tgcttgacta ttttcaagat tctgtacctc gtgaaccgga
     7261 ctatacaaaa aaaccgcctt cgtgtaaagc ggttcttaaa tcgcccgtta aggcggacac
     7321 aacgatagcc aaactggaac aggaggcggc aaaggcgggt ttgtccaaaa cagccttgat
     7381 tgaaaggctg attggggatt tgtaggcgtt tgcagatacg acaaggggcg cattccgcgc
     7441 cccatcacac gcacggacgg cttgtcatcg cctgtcgccc gtgcgcggct gcccatattt
     7501 cagaacagtc ttgcaagccc ctttaaggga acggttttat tctagtacag tttgaatgcc
     7561 ttggcaacgg ctgttttaat cgcctgaaaa tcctcttcac tgattattgg aatgcagcgg
     7621 tcgcgccctt tgggtttgta tcggtctaat cgtgccaatc cgactgttgc cgtcatgtcg
     7681 cattttgccc aacattggat gtgcggcttg tccggtaagg ggtttccact cattttgtgg
     7741 tggtagtccg ccaaagggac aggttctgtg ctgcttaagg gtacgaccgt taccagtttg
     7801 ccgttgtgcc tgtttcgcgc tatgacgacg acagggcgtt tcttgaccat ttccggttct
     7861 tcataaccgc gaaagtcgca catgataacc gaacgttccc ttggttggaa ttttaaaggc
     7921 attagccgct cctgacgatg acaggctgct attatagcag ttcttacaca aaaaaccgcc
     7981 ttcgtgtaaa gcggttgcaa aaaagccttc caataaaaat gcggaaacgg ttttttattt
     8041 gcgtttccgc ataatacaaa cacacacaca aaaaaaaatc attttccgtt tttggcaagc
     8101 ctcaaaacat cattccccca ccgttccagc gcatccttac gctcctccca aagctccgag
     8161 cggttgtaag ccgtttgcgt cctcgtcccc aacgaatggg caagcagctt ttccgccaca
     8221 tcattatcca ccttcagaac ctcgcgcaaa taagtacgcg caagcgagcg caaaccgtgc
     8281 gccgtcgtat ccagccccgc ccgctgcatc gccacgcgcg gactttcctt attgatatgc
     8341 ccttgaaaat tcacactttc aaacacaaac cgcccgttca cattcaacgc ccgcgcctga
     8401 tccaacaccc actgcatcgc ccgcgacagc ggcacatcat gcccccgccg ctcctttttc
     8461 cgttcggcgg ggatacgcca aacctccccg tccaactccg accactccat caacgccgcc
     8521 tcctgaatcc gcgtcattgt caacagcagc caatagatac aaagccgcgt tacaggatgg
     8581 atacgcaccc tgcccgcata aaccgcaaat tcattgcgct gctccaaaaa atcaatcagg
     8641 cgcggcaatt cagacggact caacgctgcc atattccccg ttttcgcccg ttcaaacaca
     8701 cccgaaccga tttgcgcgac agggttgatt tccatcattc ccgaaccgac cgcaaacgca
     8761 aacaccatct tcagactgtt tttcgttttg cgcaacgtat caacgatacc gcgcgcctcc
     8821 atcacacgga ggcagccgac cacatccgcc gtcctgacct ggcgaatatc aagattgccg
     8881 atagccggaa aaacccaccg ttcaaaattc cgcataacct gtccggcata cttttcagac
     8941 cgccccttcg accaacgcac aaaccaatca cgcgtcacct tctcaaaagc aaaatccgcc
     9001 cgcaccttct tattgacgac attttccccg tgcgcccgtt ttcggcgcac ctcctcccgc
     9061 cattcccgcg catcggccag cgaaaaatca ggataccgcc ccagcgaaat tgtctgctgc
     9121 cttccatcct gcacaaacga cagcttccaa ctccgcccgc ccgtcgggta gacccacaaa
     9181 gccagcccgc ccccgtctga caacttatac agcttatcgc gcggcttcgc atttttaacc
     9241 tgatttgccg acagcggcgt aatgattttc gccattatgg taatttccca cttatccaaa
     9301 aattaccata tacattacca taaaaaaatg caataagaag ataccgcaag attgcaaatg
     9361 atttactttg acgcgttttg atagggcaaa ccgatgattt ttatatggta attgacacaa
     9421 cttgaacaaa aaagaaccgc cctgaatcag ggcggttttg ttttctggcg gaaacggtgg
     9481 gattcgaacc cacggaggat ttgcaccctc agcggatttc gagtccgctg cattcagcct
     9541 ctctgccacg tttccgaaag atgtttcaac ctaaaaggaa acacgatgaa gtaaaaccaa
     9601 ataaaaatgc cgatgcttta tgctacttca tttttatttg gtttttgacc tcaaggattg
     9661 gcggaagcgg tgagattcga actcacggag ggctatcaac cctcgacggt tttcaagacc
     9721 gttgcattaa accgctctgc cacgcttccg tcttcttgaa gagtaggaat aataatgaag
     9781 tttatacatt ttgccaagca cttttttcgg aaaatactta atttattgtt ttcattttat
     9841 ttgttccgaa cgcataaaaa cccattcgct ttcatttgaa gccgcgtcat tgaatgcgta
     9901 gccttcgtaa ttaaaatttt tcagcatttc agggtcggta atatgatgtt ctaccgcata
     9961 gcgcaccatt aatccgcgcg cgcgcttggc atagaaactg atgattttat atttaccgtt
    10021 tttttcgtct ttaaatatcg gggtaatcag ccgcgcccga agttttttcg tgttgacaga
    10081 cttgaaatat tcctgtgagg caaggttgac aagcgtattg ctgcctgctt gggcaagcgt
    10141 gtcatttaaa aggttggtaa tgatgtcgcc ccaaaactca tacaaattct tgccgcgcaa
    10201 attggcaaat gccgtcccca tttccaaacg atagggctgt atcaggtcta acgggcgaag
    10261 aagaccgtac agaccggaca gcaggcggac atggttttgc agatagcgta tctgtccaat
    10321 atccaatgtg tttgcatcca taccttcgta aacatcgccg ttgaacataa agaccgcctg
    10381 tttggcgttt tccggcgtaa acggcgtgtg ccattctgca ttgcgctgcg cgtttaagag
    10441 ggcaattttg tcggaaacgt gcatcagttc ggcaatctgt tgcggcgcaa gctcgcgcaa
    10501 ctgctgcatt agaatgtcgg actctgccag caggtcgggt tgggtaaact cgctgacagg
    10561 ggcagggtct ttttcgttaa ggtttttcgc aggggaaagg acaaaaaaca taatctgctc
    10621 ggtatagtgt caaacacggg gattttaagt ttaaacgtgt ttgcgggcaa gccgattaga
    10681 ttaaaagtga agtaaatacc gttacagcaa attcaaccgt aagatattga aaaatgccgt
    10741 ctgaacaaaa gttcagacgg cattttttta ttctttaccg atatagggaa acaatggttt
    10801 ccgcagcttt gacacaagtt gcgacatcgg caaccaaagc gatacgcaca taaccttccc
    10861 cgggattgcc ctgttcggta tcccgtgcca aaaagcgtcc gggcaatact tggatagcgg
    10921 ctttttgcca taaattgcgt gcaaatgcca aatcgtcgcc atcggggact ttcaaccaaa
    10981 tgtaaaacga ggcatctggt aatttaacat caaatacctg ttgcaaaatg ggaataacgc
    11041 gctcaaattt ttcctgatac aggcggcggt tgtcgataac gtgctgttcg tcatcccaag
    11101 cggcaatgct tgcgcgctgc acgggaatgc tcattgcact gccgtgatag gttctgtaaa
    11161 gcagaaagtt tttaagcagt tcggcatcgc cggcgacaaa accggaacgc aggcccggaa
    11221 cgttggaacg cttggacaaa ctggtgaaca taagcagttt ttgcctgctt cgacccaact
    11281 gtgcagcggc ctgcaggcag cctaaaggtt tgttgccgtc gaaatagatt tcggaatagc
    11341 attcatccga ggcaataatg aaaccatatt tatcctgtaa atcaaaaact tctttccatc
    11401 catccaaatc cagcacgctg ccgctggggt tgttgggcga gcagacgaac accagtttgg
    11461 tgcgtttcca aacctcttcg gaaatactac gccaatcggg gttgaaagac ggcgcggggc
    11521 aattggcaaa atggatttca ccgccgccca aaagtgtcgc accttcgtaa atctgataaa
    11581 agggattcgg gctgacaatt gcgggtttga tgccgtctga aacagggtcc aacacggttt
    11641 gaacaaaaga aaacaacgcc tccctactgc ctaaaaccgg cagaatttca ttatccgcat
    11701 ccactgtcaa gccatcataa cggcgtttta accagttcgc acacgcctga cgcagttcag
    11761 gcaaaccggc cgtcagcgga tatttttcca actcgtgcaa tgaggcggtc agcgcatctg
    11821 taatgacttt cggtgttgga tgtttcggtt cgccgatatg cagtgggacg gcttccagac
    11881 cttcgggggc ggaaatgccc tgcatcgctt cacgcagtcg ggcaaaggga tagggtttga
    11941 gttggttgag taaggtattc ataatgttct ccggttgtcg gtatatcgga agatatgccg
    12001 tctgaacctg acggcaccca tcagacggca ttttatattc aatgcaaacc cgtactgccg
    12061 aaaccgcctt ctccacggct gcttccgaca aattcttcga cacgtttgaa acgcgcctgc
    12121 acgattggaa cgataaccat ctgcgcgata cgctcaaacg gcttgacggc aaacggttcg
    12181 ctgcttctgt tccataacga caccttcaat tccccttgat aatcggagtc aatcaaaccg
    12241 accaaattgc ccaaaacaat gccgtgttta tgccccaagc cggaacgggg cagcaaaacg
    12301 gcggcatatg aaggattggc caaataaatt gccaaacctg tcggcacaag aaacgtttcc
    12361 cccggctgca agacgacttc ctcatccaaa caggcgcgca agtccaaacc ggcagaaccc
    12421 gctgtcgcat aggtagggat aaaatccgcc atcctctcgt ccaatacttt catttctact
    12481 tcaatattca tcgtcttgct cctgcatacg gccggtttca aagatgggag attatacaca
    12541 agccatgttt acgctgaaac acagcaaatc ggttttgttt tgaaatccgt tgccaatgta
    12601 taatacccgc ccttccggtt atgaagacga acattatgca cacactaagg caacttatca
    12661 gaaatgactg aaactcaaaa aactggattc ccactttcgt gggaatgacg tggtgcaggt
    12721 ttccgtacga atggattcgt cattcccgcg caggcgggaa tctagacctg tcggtttcgg
    12781 tttttttggc tagtgccgca acattaaatt tct
//
LOCUS       DAXHMX010000050        12597 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHMX010000050 DAXHMX010000000
VERSION     DAXHMX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.050"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(75..1496)
                     /locus_tag="ACFM4S_001012"
     CDS             complement(75..1496)
                     /locus_tag="ACFM4S_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6050715.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1854..2213)
                     /locus_tag="ACFM4S_001013"
     CDS             complement(1854..2213)
                     /locus_tag="ACFM4S_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6050716.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2210..2815)
                     /locus_tag="ACFM4S_001014"
     CDS             complement(2210..2815)
                     /locus_tag="ACFM4S_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6050717.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDTSAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2862..3344)
                     /locus_tag="ACFM4S_001015"
     CDS             complement(2862..3344)
                     /locus_tag="ACFM4S_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6050718.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3548..3645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3855..4955
                     /gene="gcvT"
                     /locus_tag="ACFM4S_001016"
     CDS             3855..4955
                     /gene="gcvT"
                     /locus_tag="ACFM4S_001016"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6050719.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5117..5503
                     /gene="gcvH"
                     /locus_tag="ACFM4S_001017"
     CDS             5117..5503
                     /gene="gcvH"
                     /locus_tag="ACFM4S_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6050720.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            5655..6902
                     /gene="hemA"
                     /locus_tag="ACFM4S_001018"
     CDS             5655..6902
                     /gene="hemA"
                     /locus_tag="ACFM4S_001018"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6050721.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSKAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7229..7750
                     /locus_tag="ACFM4S_001019"
     CDS             7229..7750
                     /locus_tag="ACFM4S_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6050722.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMALA
                     KDGTIAGAKLVDHHESIMLIGIPHLPAPGRAIRSNWLPAYIKPNFISTNRLRLKGLPT
                     VLQPSKGTGAGVP"
     gene            7639..8838
                     /locus_tag="ACFM4S_001020"
     CDS             7639..8838
                     /locus_tag="ACFM4S_001020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6050723.1"
                     /translation="MASGVYQTKLYIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPHALIEHNNILDNSVGVYLHGSSEAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTYNTYKNNRFSDLRF
                     AVHYMYTNDSEISGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLFANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDELLKEAETRQSEWGR
                     AENGSLN"
     gene            8896..9510
                     /locus_tag="ACFM4S_001021"
                     /pseudo
     CDS             8896..9510
                     /locus_tag="ACFM4S_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            9527..9658
                     /locus_tag="ACFM4S_001022"
                     /pseudo
     CDS             9527..9658
                     /locus_tag="ACFM4S_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9655..10149
                     /locus_tag="ACFM4S_001023"
     CDS             9655..10149
                     /locus_tag="ACFM4S_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6050724.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGSPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(10237..11898)
                     /locus_tag="ACFM4S_001024"
     CDS             complement(10237..11898)
                     /locus_tag="ACFM4S_001024"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6050725.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQVIERFQLDQNSQPQTYGLGIKE
                     IWEVPSEKHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVENFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(12028..>12597)
                     /locus_tag="ACFM4S_001025"
     CDS             complement(12028..>12597)
                     /locus_tag="ACFM4S_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6050726.1"
                     /translation="NPVAYGKIKVQSWKERRDFNIVKQDLDFSCGAASVVTLLNNFYG
                     QKLTEEDVLKKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKY
                     RKDDHFSVLRGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRD
                     AGGDKAFFTRSPKRQTEFAVGQVKWWRAY"
BASE COUNT         3132 a         3177 c         3390 g         2898 t
ORIGIN      
        1 gtcggaaggg aaaaatattg atttgattat gccggaattt aaaatgccgt ctgaatgttc
       61 agacggcata gcgtttacag cagtttgaaa acgaaaaaga taagggtatg tacgatgaag
      121 acgggtgtca ggaaggcgac cgaccacatc atatagccga agaaagtcgg catcggtacg
      181 ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg cgttgccgat gtaggtcagt
      241 gcgcccatga ataccgaacc catagaaacc gccagcagcg aatgaaacag ggtacccgtc
      301 atcaaggctt gggcatcgcc gcccgccata ttgaaaaaaa cgagataagt gggcgcgtta
      361 tccaagaatg ccgacaatat gccgctcatc caaaaataca tcacattaat cggatgacct
      421 gccgtatcgt gaaccagcga taccaccccg cccagcgcgc ctgcctcgcc tgctttcaga
      481 atgctcagga cgggaaagat ggtgatgaag atgccgagga agagtttgcc cacttcggcg
      541 atgggttcaa agttgaattc gttgcctgcg cggacttgtt tgggcgtgat tgccatagat
      601 acggcggtca atgcaatcag gatgacatcg cggacgaggt tttgcagggc gtaacggctg
      661 ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc cggacattag aaccgcgccg
      721 accacgcccg aaagcaggag gaagttccat ttgccgaaga tggcgatttt ttcgggtttt
      781 tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt tgaagaaacg gttgtcgatg
      841 aaatagaagg cggtcaacag gacagcggtg ctgatcagga cgggggcgaa catatgtttg
      901 accgtccaca tgaaatctac gcctttgagg aagccgagga agagtggggg gtcgcccaaa
      961 ggggtcagac cgccgccgat gtttgcaacc aggaaaatga agaagatgac gatgtgcacg
     1021 cggcgggtac ggttttggtt ggctttcagc agcggacgaa tcatcagcat tgctgcgccg
     1081 gtcgttccca tgatagaggc aagtgccgta ccgacggcaa gcagggcggt gttgagcttg
     1141 ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg aaatggtgta cagggcaagc
     1201 agcagcagga tgaaggggat gtattcttca acgagtgcgt gtgcgacggt atggataccg
     1261 gcggacgcgc caaaaaccaa actgaacggg atgaggaaga gcaatgtcca aaaggcggtg
     1321 attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca agggacccaa tgcgatagac
     1381 agcaaaatca gggcaaaggg caggctccac agcaggttta ggtttgcgcc gtccaaatct
     1441 gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca ggggtaggtg gcgcatcgtg
     1501 tttcctcgat tcaagcactg ccttgcgcgg cgcgtgggag tgatacaggc accgtgccgc
     1561 ccggacatag gcggactgtg ctgcctgttt gtttttgaaa aaagttttcc gattgattgt
     1621 aaagtaaata atcaggataa accagtttcc caaaccggaa ggcggcggga aggcggattg
     1681 ctgtgcttgg gaatatatgt tctttttgat aaataatttt atttaaaaca aataaatata
     1741 tgtctttaat aaataatcta tcgaaaacga aaaatgaatt tattttaaca tatatttgca
     1801 atgaaacagg tttgcccccc gtttgtttgc ccttatccct ttcagtacgg cattcaagat
     1861 tcgggcctgc gccacatcca tatggcgaca agggaacaaa aaaccgatga aaccgccccg
     1921 acccaccagc gttggggaaa ctgccaaaac attatcaggc aggatgcggt catcatactg
     1981 atggcgaata ttttggcttt gcgcggcact gcgccgtttt gttcccagtt atgaaccatc
     2041 ggaccgaaat agcggtgccg gtgcagccag cggtgaaagc gcggggatgc ctttgcccag
     2101 caggcggcgg agagcagtac gaacggcgtg gtcggcaaca gcggcaaaaa aatgccgatg
     2161 atacccaaca gcagggaaat gcagccgcag gcaattaaaa gataacgtat cattttgaaa
     2221 tatttttctt attgtgcgga taagggcagg atgtgatacc gagttttgcc cagccttcat
     2281 gtcccatttt ttccagcagg gcgatattgc gttcgaatat tgccgaagtg tcgggaaagg
     2341 cttgtgcggc tttggcaatg ctgtcttcgc ggatgaggtg cagcgtcgga tagggagaac
     2401 ggttggtgta gttgccgatg tcgtcgggtt cggtattctg aaagcggaat tggggatgga
     2461 acggtgcgat ttggacgatg ccttccaagc cgttttcgat aacggcggca tcggcaatat
     2521 cgagcatatc gttgaatacg tcgaaatcgg ggaatagggt cgggtgaacc agcagggtgg
     2581 tttccagttc ggtggcgggt gtattgccca gtcgctgcag ttcttcgtcc aagtcttcca
     2641 aaaaactgtc aaggtgtttg gcttcgctga ttgcgatgcg gacaaggttt ttaacgtggg
     2701 gggctttggc aaagggacac aggttcagac cgatgacggc tttttccaac cattgtccgg
     2761 tgtgttcggc aacagcatct ttattttcgg aagtattgat attcattatt gtcatgtaaa
     2821 tgtgtttgca gattgcacgt gcgggaaaat cgggaagggc actattcctt cagcaggtgg
     2881 ttgagcggca gggaggtggt gtgtttgatt tcttttaaaa caaagctcga ttgcgcatct
     2941 tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat gggaaaacgc gttcatatcg
     3001 gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca gggcgaagca gctcaagact
     3061 tcaggccatt ttcgaaccga tgcggcaaag tcttcccgcg cgtcttttgc tttgcggatg
     3121 gaaacgcgga taaatgcctg aagccccagg ttgacggatt ccggagacag cagcgcggca
     3181 tattggcgga cgatgccggc atcttccaac tgcttcagac ggcgcaggca cggagaaggc
     3241 gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc tgccgttttc ctggagaacc
     3301 tgtaagattt taatatcggt tttgtctaaa gtgagttggg gcatatttgc gttccgtttt
     3361 aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc gcacagatgg agaccatatt
     3421 aacatataaa aagttatacc gtcatccggg acaaattttg ttttcggaaa atcatgtgaa
     3481 aacagaggcg gtcggtttgc atctctttta agacggcttg cccaaaccgc cgattcaaga
     3541 cataatcggg aaatgtgcag gagagtgtta cacccaacta caatgtaacc accgaaggcg
     3601 cagacaccct taaatcgctc aggtatcagg gactgcacat tgaaacaaac aatctggaga
     3661 gcggcgttgg aataacgtcc accgaagggg agaaggccgt ctgaaccacc attcagacaa
     3721 ccgcgcaaag cagtgagcag actggtttgc catcatgcgg atacggccga aaatctcagg
     3781 ttcaaggaca gatagggtca tccgcgcatt ttgtgcggac ggcatctgaa caaaaaatcc
     3841 ggagaaactt gagaatgatt actctgaaaa ccaccccatt tcatcaagcc catcaagatg
     3901 caggcgcgaa gccggtagat tttgccggct gggagctgcc catccattat ggttcgcaaa
     3961 tcgccgaaca cgaagccgtg cgcaccgacg ccggtatgtt tgacgtatcc catatgcttg
     4021 ttaccgacgt agcaggcgca aatgccaaag cctttttccg caaattgatt gccaacgatg
     4081 tcgccaagct cgcttttgtc ggcaaagccc tttattccgc tttgctcaac gacaacggcg
     4141 gcgtgattga cgacttaatc gtttaccgca ccaacgaagc cgaaacccaa taccgcatcg
     4201 tgtccaacgg agcgactcgc gaaaaagaca cggcgcaatt ccacaaagtc ggacaagagt
     4261 tcggcgttgc ctttaatccg cgctacgacc tcggcatgct cgccgtacaa ggccctaaag
     4321 ccatcgaaaa actcctgacc gtcaaacctg aatgggcgga tgtgattcac ggcctcaaac
     4381 cgttccaagg tgcagacttg ggcaacgatt ggtttgtcgc ccgcaccggc tataccggcg
     4441 aagacggcgt cgaagtgatt ctgccgggca ctgaagccgt cgcattcttc aaagccctgc
     4501 aagcagcagg cgtacaacct tgcggtctcg gcgcgcgcga caccctgcgc atggaagccg
     4561 gcatgaacct ctacggcaac gatatggacg acgataccag cccgctcgaa gcaggcatgg
     4621 gttggaccgt tgatttgaaa gacgaaagcc gcgattttgt cggcaaagct gccttgttgg
     4681 cattgaaaga aaaaggcgtt gccgtcaaac aggtcggttt gctgctcggc aaaggcggca
     4741 tcctgcgcgc gcatatggaa gtgttgaccg acaaaggcaa aggcgaaacc accagcggcg
     4801 tattctcgcc cagcctgaaa caatccatcg ccatcgcccg cgtaccgaaa gactttgacg
     4861 gcgataccgc taaagtgctg atgcgtggca aggaagtgga cgtgcgcgta ttgaagttgc
     4921 cgttcgtgcg taacggtcaa aaacagtttg attaatatcg gttcagacgg cattttcatt
     4981 tcatatgccg tctgaaagca ggttttaatt gttgtccgat acggacgttt gtagaaagca
     5041 ttgaacaagg catctgtgga tattgattca tgcagatgcc gtctgaaaat aacccctatc
     5101 aatggagtat caaaccatga gcaacaacat cccggccgaa ctgaaatacg ttgccagcca
     5161 tgaatggctg cgccttgaag aagacggtac gattaccgtc ggcattaccc accacgcgca
     5221 agagctgttg ggcgacatcg tgttcgtcga gctgcccgaa gtcggcgcga accttgccgc
     5281 tgaagagcaa gccggtgtgg ttgagtctgt aaaagccgcg tccgacgtgt acgcaccgat
     5341 tgcaggcgaa gtcgttgccg tcaacgaaga tttgccaagt gctccggaaa ctgccaacag
     5401 cgattcttac ggcgcaggct ggttcttcaa aatcaaacct gtcaatcctg ccgattacga
     5461 cggtctgctg actgccgaac aatacgcggg cgaagtggat taaaccgccc ggctgcccga
     5521 cggcaaccgc cggacaaacg gaaactgcac cttcagacgg catttttgcg gtcggaggtg
     5581 cagttttttg tccgtgtttt aaggaagcag ttaggctata ataacggcct atattcatct
     5641 ttaccgattt tttcatgcaa cttaccgctg tcggactcaa tcatcaaacc gcacctttaa
     5701 gcatacggga aaagctggcg tttgccgcgg cctgcctgcc cgaagccgtc cgcaatcttg
     5761 cccgaagcaa ggcggcaacg gaggcggtaa tcctttctac ctgcaaccgc accgagcttt
     5821 actgtgtagg cgattcggaa gaaatcatcc gttggctcgc agactatcac agccttccca
     5881 tagaagaaat cagcccctac ctttatactt tggggatgca ggagactgtg cgccatgcct
     5941 tccgcgttgc ctgcggcttg gattcgatgg tgttgggcga gccgcagatt ttaggacaga
     6001 ttaaggatgc ggtcagggtt gctcaagagc aggaaagtat gggtaagaaa ctcaatgccc
     6061 tgttccaaaa aaccttttct gttgctaaag aggtccgtac cgatactgcc gtcggcgaaa
     6121 actcggtttc catggcttcc gcttccgtca aattggcgga acagattttt cccgacatcg
     6181 gcgatttgaa tgtcttgttt atcggcgcag gcgaaatgat tgagctggtt gccacttatt
     6241 ttgccgccaa aagtccccgg ctgatgacgg ttgccaaccg gacgctggcg cgtgcacagg
     6301 agttgtgcga caagctcggt gtcaacgccg aaccgtgcct gctgtccgat ctgcctgcta
     6361 ttctgcacga ttacgacgtg gtggtttctt caacggcgag ccagcttccg atagtcggca
     6421 aaggcatggt cgaacgtgca ttgaaacagc gtcagagtat gccgttgttt atgcttgatt
     6481 tggccgtgcc gcgcgatatt gaagcggaag tcggcgattt gaacgatgcc tatctttata
     6541 cggtggacga tatggtcaac atcgtccaaa gcggcaagga ggcaaggcag aaggccgccg
     6601 ccgccgccga aacgctggta tccgagaagg ttgccgaatt tgtcaggcag cagcagggca
     6661 ggcagagcgt ccccttgatt aaagccttgc gggacgaggg cgagaaagcg cgcaagcagg
     6721 tgttggaaaa tgcgatgaaa cagcttgcca aaggcgcaac ggcagaagag gttttggaac
     6781 ggctgtccgt ccaactgacc aacaagctgc tgcattcgcc gacccaaact ttgaataagg
     6841 cgggggaaga agataaagat ttggttcatg ccgtcgcgca gatttatcat ttggacaaat
     6901 aacggtgcgc cgggaaaaat gccgtctgaa gaggtttcag acggcatttt tttgtgccgc
     6961 ctgacaacat cgtgaaatcc cacattatat cgatgtaatc acaaagtata gtggattaac
     7021 aaaaatcagg acaaggcgac gaagccgcag acagtacaga tagtacggca aggcgaggca
     7081 acgctgtact ggtttaaatt taatccacta tattatcccg tatgcggatt ggttttaaga
     7141 tttgtaaatt taatttgcat caaaaaatcg ccgatagatg attcatataa tatcaatatt
     7201 aaagagtatc ggtatatcgg ggatagtcat gtcctgtttt tcaatcaaac gtatgtccgc
     7261 gtttcgggcg cggataacgg cgttttttgc cgcctttgtc tttttgacgg cggcactgcc
     7321 cgcttatgcg gagcgtctgc ctgattttct ggcgaaaata cagccttcgg aaatttttcc
     7381 gggtgcggac cgttacggca agccggaagg taagcctatg gttgcccgcg tttacaaagg
     7441 cgatgagcag ttgggcttgg tctatatcac gaccgatgcg gtcaatacgc gcggttattc
     7501 gagcaaaccg attgatacgc tgatggcgtt ggctaaagac ggtacgatag ccggagcgaa
     7561 attggttgat caccatgagt cgattatgct gatcggtatc ccgcatttgc ccgcgcccgg
     7621 gcgggcgata cgctcaaact ggcttccggc gtatatcaaa ccaaacttta tatcgacaaa
     7681 ccgattacga ttgaagggcc tgccgaccgt tctgcaacca tcgaagggga caggagcggg
     7741 cgtaccatag ccgtacacgc gccggacgta acgctccgca acctgaccgt tacccgttcc
     7801 ggcatgagcc tgccagcaat ggatgccggt atttatctcg aagaaaccgc tccgcacgcc
     7861 ctgattgaac acaataatat tttggataat tcggtcggcg tctatctgca tggttcttcc
     7921 gaagcgatgg tgcgcgagaa caaaatcgtc ggcgacgcga ctttgcgcgt gaacgagcgc
     7981 ggcaacggcg ttaccgtttg gaacgcgccc ggtgcgcaag tcgtcggcaa cgacatttcc
     8041 aaagggcggg acggcatttt ttccaacacc agcacatata acacctacaa aaacaaccgc
     8101 ttcagcgatt tgcgtttcgc cgtccactat atgtacacca acgacagcga aatcagcggc
     8161 aatatttccg tgggcaacaa tatgggctat gtgctgatgt tttccgagcg gctcaaagta
     8221 ttcgacaata tcgccgtcgg cagccgcgat cagggcatta tgctcaacta tgtcaactat
     8281 tccgatattc acgacaacat tatcaacaaa gcgggcaagt gcgtatttgc ctataatgcc
     8341 aactacgata aacttttcgc caatcatttt gaaaactgcc aaatcggcat acactttacc
     8401 gccgccatcg aaggcacgtc cctgcacgac aattccttca tcaacaacga aagccaggtc
     8461 aaatacgtca gcacgcgctt tctcgactgg agcgagggcg gacacggcaa ctattggagc
     8521 gacaacagcg cgttcgattt gaacggcgac ggcttcggcg acagcgcgta ccggcccaac
     8581 ggcatcatcg accaaatcat ctggcgcgcg cccgtatcgc gtctcttgat gaacagtccc
     8641 gcaatcagca tcgtcaaatg ggcgcaggcg cagtttcccg ccgttctgcc tggcggcgtg
     8701 gtggacagca aaccgctgat gaagccttat gcccccaaaa ttcaaacccg ttatcaggcg
     8761 atgaaggacg agttgctcaa agaagccgaa acgcggcagt cggaatgggg cagggcggaa
     8821 aacgggtctt tgaactagtc tgtttcagac ggcatccgga ttcaaatgcc gtctgaaaac
     8881 acaaaaggaa caaccatgac cacacatcat gtcgaattga ggaaggtaac caaacggttc
     8941 ggggcgcaaa aagccgttaa ccaagtcgat ttggttttga aggcaggaga aagcgtcggg
     9001 cttgccggac acaacggcgc gggcaagtcc accattatga agctgatact cgggctgatt
     9061 accccgaccg aaggcgaagt gatgcttttg ggcgaacgta ccggcagcaa agcgggggcg
     9121 cggcttcgca gccaaatcgg ctacctgccc gaaactgttg cgttgcaccc ttcgctgacc
     9181 ggcatcgaaa cgctggattt ttatgccaaa cttaaaaaac agccgctcac gcagaatcgg
     9241 gggctgctcg agcgcgtcgg catttcacag gcggcacacc gccgcgtcgg cacttattct
     9301 aaagggatgc gccaacgcct tgccttggca caagccctgc tgggcgagcc caaagtcctg
     9361 ctgtttgacg aaccgacaac cggcctcgac cctgcatcaa ggcaaatgtt ttacgaagtc
     9421 gtgcgcgaac tcaacgggcg cggcgcgacc gtattgctca gtacccacgc ccttgccgag
     9481 ttggacgggc acgccgaccg cattatcgtg gattaaattt aatccactat atgcgggtat
     9541 ggcgggtttg agcggacaaa tcagcctgac cgtccccgtt ttgctgaccg ctcaggtttt
     9601 atgggttatc attccgcttg ttttggcagc cggaattttt agaaagcgac aaatatgaaa
     9661 aaaaccctgt tggcaattgt tgccgtttcc gccttaagtg cctgccggca ggcggaagag
     9721 ggatcgccgc ctttaccccg gcagattagc gaccgttcgg tcggacacta ttgcagtatg
     9781 aacctgaccg aacacaacgg ccccaaagcc cagattttct tgaacggcaa acccgatcag
     9841 cccgtttggt tctccaccat caagcagatg ttcggctata ccaagctgcc cgaagagcct
     9901 aaaggcatcc gcgtgattta cgttaccgat atgggcaatg ttaccgattg gacgaatccc
     9961 aatgccgaca cggagtggat ggatgcgaaa aaagcctttt acgtcatcga cagcggcttt
    10021 atcggcggta tgggtgcgga agacgcgctg ccgttcggca acaaagagca ggctgagaaa
    10081 tttgcaaagg ataaaggcgg taaggttgtc ggtttcgacg atatgcctga tacctatatt
    10141 ttcaaataat attgaaaaaa ccggccgaaa ctttaaaaac gagtttcggt cggtttttct
    10201 tttcttgttc ggtatagtgt cggcaggaaa gaaccttcac atcccgccgt aattcggccc
    10261 gctcgcgcct tcggggcaaa tccaagtgat gttttgcgtc gggtctttga tatcgcaggt
    10321 tttgcagtgc acgcagtttg ccgcgttgat ttgcaggcgc ggattgccgt tttcttcaac
    10381 aatttcgtac acgccggccg gacaatagcg cgtttcgggc gaggcgtatt ctttgtagtt
    10441 cacgtctatc atcgtttgcg gattgttcag caccaaatgg tcgggctggt tttcttcgtg
    10501 cgcgagattg gcaaggaaga cgctgctcaa gcggtcgaag gtcaacacgc cgtcgggttt
    10561 cggataatca atcggcttgc acgcggcggc ttttttgagc tgctcgttgt ctttgccgtg
    10621 atgtttcaag gtccacgggg ctttgcctct gaaaatcatc tgatcgatgc cggtgtagat
    10681 tgagccgagg taaacgcccc atttgaatga cggacggaca ttgcgcgcgg cgtaaagctc
    10741 ttgatacagc cagctttgtt caaaacgttg ctgataatcc gccgcctctt tgccgctgtc
    10801 gaagttttcc acttcttcaa ggttttccaa caaggggaac acggcttctg cggcgagcat
    10861 ggcggatttc atcgcggtat gaatgccttt gatgcgcggc atattgagga agcctgccgc
    10921 gtcgccgatt aaaacgccgc ctttgaacga gagcttcggc aaactttgca aaccgccttc
    10981 aatcagcgaa cgcgcgccgt aagcgatgcg gcggccgcct tcaaaggttt tgcggatttc
    11041 gggatgggtt ttgaaacgtt ggaactcttc aaacggcgac agataagggt tttgatagtc
    11101 caaaccgacc acgaaaccga cggcgacttt gttgtcatcg aaatggtaaa caaacgcgcc
    11161 gccgtaggtt ttgctgtcca gcggccagcc cgcgctgtgc accaccaagc cgggctgatg
    11221 tttttcagac ggcacttccc aaatttcttt gatgcccaag ccgtaagttt gcggctggct
    11281 gttttggtcg agttggaaac gttcgatgac ttgtttggaa agcgaaccgc gacaaccttc
    11341 ggcaaacaga gtttgctgcg cccaaagctc catgccgggc tggaaactgt cggtcggctc
    11401 gccgtctttg ccaatgccca tattgccggt tgcaatgcct ttgaccgaac cgtcttcgtg
    11461 atacagcact tcggcggcgg caaagcccgg ataaatttcc acgcccaaat tttccgcctg
    11521 ctccgccaac cagcgcacga cttcgcccaa gctgacgatg tagttgccgt gattgtcgaa
    11581 atttggggta ataggcagat tgaacgcttt tttctcagtc aggaacaaca ctttgtcctg
    11641 cgttaccgta cgcgtcagcg gcgcgccttt ttctttccag tcgggaatca actcattcag
    11701 cgcaatcgga tcgataaccg cgcccgccag cgaatgtgca cccgcctccg aacctttctc
    11761 caccacgcaa acgctgattt cgcgcccatt ttgttcggca agctgcttga gtttgatggc
    11821 ggcggacaaa cccgatgggc ctgcgccgac aatcacgaca tcgtattgca tactgtcgcg
    11881 ggtgatggat tctgtcatgg cggttcctgt gtatttatta ttgaattgca aatccgtaat
    11941 tatacaacga gaacatatag ttaccaaata caacaaaggt cgtctgaaaa ccatattttc
    12001 ggttttcaga cgacctttgt cgaaatttca ataagcacgc caccatttta cctgtccgac
    12061 cgcaaactcc gtctgacgtt tcggactgcg tgtgaaaaac gccttatccc cgccggcatc
    12121 cctccctttc ggcacaaccg ccaaaatctt acctgccaaa tttccctcac gggtttgcca
    12181 agcatccaaa aactgcgccc tgctcattga aacatgaccc agcgacgggt cggcaagcaa
    12241 aaccgtattg ccgtctatac cgcgcaatac cgagaaatga tcatccttgc ggtatttcag
    12301 atacacgatg acggggattt gcaactgtgc aagctgctcg aaagacaggg catagccttt
    12361 cgcttcaaaa cccaaatcag gcataatgcg ccgcatatcc tcaaacgacg cgggcatctg
    12421 ctccttatcc agttttttta acacgtcctc ttccgtcagc ttttgcccgt aaaaattgtt
    12481 caaaagcgtc accaccgaag ccgccccgca ggaaaaatcc aaatcctgct ttacaatatt
    12541 gaaatcgcgc ctttctttcc aactctgcac tttgattttt ccataagcaa caggatt
//
LOCUS       DAXHMX010000051        12317 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHMX010000051 DAXHMX010000000
VERSION     DAXHMX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.051"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            199..1122
                     /locus_tag="ACFM4S_001026"
     CDS             199..1122
                     /locus_tag="ACFM4S_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6050727.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1187..2575)
                     /gene="fumC"
                     /locus_tag="ACFM4S_001027"
     CDS             complement(1187..2575)
                     /gene="fumC"
                     /locus_tag="ACFM4S_001027"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6050728.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2716..4695)
                     /gene="tkt"
                     /locus_tag="ACFM4S_001028"
     CDS             complement(2716..4695)
                     /gene="tkt"
                     /locus_tag="ACFM4S_001028"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6050729.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DGYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQATVLPEGLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKEFGFTVDNVVDTVKSVL"
     gene            5222..6718
                     /gene="ccoG"
                     /locus_tag="ACFM4S_001029"
     CDS             5222..6718
                     /gene="ccoG"
                     /locus_tag="ACFM4S_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6050730.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6721..7269
                     /locus_tag="ACFM4S_001030"
     CDS             6721..7269
                     /locus_tag="ACFM4S_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6050731.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(7611..8786)
                     /locus_tag="ACFM4S_001031"
     CDS             complement(7611..8786)
                     /locus_tag="ACFM4S_001031"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6050732.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKYVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(9345..9419)
                     /locus_tag="ACFM4S_001032"
     tRNA            complement(9345..9419)
                     /locus_tag="ACFM4S_001032"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9384..9386),aa:Glu,seq:ttc)
     gene            complement(9437..9513)
                     /locus_tag="ACFM4S_001033"
     tRNA            complement(9437..9513)
                     /locus_tag="ACFM4S_001033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9477..9479),aa:Arg,seq:acg)
     gene            9631..11016
                     /locus_tag="ACFM4S_001034"
     CDS             9631..11016
                     /locus_tag="ACFM4S_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6050733.1"
                     /translation="MIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLREQLYGLPDS
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKASFDWCNTEAVRFFPALGRLHAYK
                     IKAELVRRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTTALKNGVTDNPPTGLFA
                     NKKAARRALSSWAETYGLCPAAAGILSDGYTEDEPCPVYVSGRCDKACGRSDEQVLAF
                     AHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKDLPDAFKAKF
                     KTDRKNIKEIG"
     gene            complement(11227..12006)
                     /locus_tag="ACFM4S_001035"
     CDS             complement(11227..12006)
                     /locus_tag="ACFM4S_001035"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6050734.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALAEHPLVGEYGHSFRFVPVTTRAANPSGLGGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPEIVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
BASE COUNT         2750 a         2849 c         3542 g         3176 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatttt aaaattcagg cggtgttttc aaatagcgcg
       61 aagcaatagc cgagcttcag ccggtttgtt tgaaatcaaa gggttggaat caaacgccaa
      121 acgcttgaac catcattgcc cgatgcggca aaccggcatc gtccggcaat acggttgttt
      181 aaaaatagga aaatcaggat ggaaaaccaa aggccgctcc taggcttcgc gttggcactt
      241 ttggcggcga tgacgtgggg aacgctgccg attgccgtgc ggcaggtatt gaagtttgtc
      301 gatgcgccga cgctggtgtg ggtgcgtttt accgtggcgg cggcggtatt gtttgttttg
      361 ctggcactgg gcgggcggct gccgaagcgg cgggattttt cttggtgctc attcaggctg
      421 ctgctgctcg gcgtggcggg catttcggca aactttgtgc tgattgccca agggctgcat
      481 tatatttcgc cgaccacgac gcaggttttg tggcagattt cgccgtttac gatgattgtt
      541 gtcggtgtgt tggtgtttaa agaccggatg actgccgctc agaaaatcgg cttggttttg
      601 ctgcttgccg gtttgcttat gttttttaac gataaattcg gcgagttgtc gggtttgggc
      661 gcgtatgcga agggcgtgtt gctgtgtgcg gcaggcagta tggcatgggt gtgttatgcc
      721 gtggcgcaaa agctgctgtc ggcgcaattc gggccgcaac agattctgct gttgatttat
      781 gcggcaagtg ccgccgtgtt cctgccgttt gccgaaccgg cacacatcgg aagtttggac
      841 ggtacgttgg cgtgggtttg ttttgcgtat tgctgcttga atacgttaat cggttacggc
      901 tcgttcggcg aggcgttgaa acattgggag gcttccaaag tcagcgcggt aacaaccttg
      961 ctccccgtgt ttaccgtaat attttctttg ctcgggcatt atgtgatgcc tgatactttt
     1021 gccgcgccgg atatgaacgg tttgggttat gttggcgcac tggtcgtggt cgggggtgcg
     1081 gttacggcgg cggtggggga caggctgttc aaacgccgct agttcgcagg caacggaaaa
     1141 tgccgtctga acgaggtttc agacggcatt ttatttgagg gaaagattag cgcggatgaa
     1201 ccatatcggc aggaacgacc agttcgtcaa attcttcgcc cgtcagcaag cccaactcaa
     1261 cggcggtttc gcgcagcgat ttgtcgtttt tgtaggcggt tttggcgact ttggcggcgt
     1321 tttcgtaacc gattttgcgg tttaacgcgg taacgagcat cagggaatgg tgcaggaaat
     1381 agtcgatttt ttccggtacg ggttcgatgc cgacggcgca gtgttcgttg aagctgttgc
     1441 acgcgtcgcc caagaggcgg atggattgca agaggttgta ggcgataacg ggcatataga
     1501 cgttcagctc gaaattgccc gacgcgcccg ccataccgat ggtaacgtcg ttgccgaaca
     1561 cttggcagca caccatcgtc attgcttcgc attgggtcgg gttgactttg cccggcatga
     1621 tggacgaacc cggctcgttt tcggggattt tgatttcgcc caaaccgcaa cgcgggccgc
     1681 ttgccagcca gcggatgtcg ttggcgattt tattcaggct tgccgccagc gttttcaatg
     1741 cgcccgaagc ggcaacggcg gcatcgcgtc cgcccaaggc ttcaaatttg ttcggcgcgc
     1801 tgacaaacgg caagccagac agttcggcga gtttagcagc agctttttcg gcgtattcgg
     1861 gatggctgtt caaacccgtg ccgaccgccg taccgcccaa agcaagttca tacaagtctt
     1921 taagcgcatc gttcagacgg cctaaaccgt gatcaagctg ggaaacgtag ccggaaaatt
     1981 cctgtcccaa agtcagcggc gtcgcgtctt gcaagtgggt gcggccgatt ttaacaatgg
     2041 gggcgaaagc ttgggctttt ttgtccaagg tatcgcgcag ggcttttacg gcggggatga
     2101 ggtggcggtt gatttcaatc gcggcggcaa cgtggatagc ggtcgggaat gcgtcgttgg
     2161 tcgattgcgc gtggttcaca tggtcgttgg gatggacggg ctgataagcc gccaaacccg
     2221 taccggcgat ttcgttggcg cggtttgcca gcacttcgtt catgttcata ttggactgcg
     2281 tgccggagcc ggtctgccaa accactaaag gaaactgtcc gtcaagcctg ccgttcaaca
     2341 catcatccgc cgcctgcgta atcaaatccg cctgttccgg cttaatcctg ccgagggaaa
     2401 cattggtcgc ggcagcggct tttttcacca acgccaaagc ataaatcagc ggctgcggca
     2461 gggtttcgcc gccgattttg aaattgttgc ggctgcgctg ggtctgcgcg ccccaatagg
     2521 cttcggacgg gacttcgaca ttgcccatgg tgtcgtgttc ggtgcgagtg ttcatacgtt
     2581 tctccttttg aaatgtgaat aagagtgatt cgcaaatatt ataatggaga ttggtggaat
     2641 gaggaagcat taaggaggga gggtgtgaaa atgccgtctg aaaagcgttc agacggcatt
     2701 tgtttaggtg tgggtttaca gcacggattt cactgtatcg accacattgt ccacggtaaa
     2761 gccgaattct ttgaagagta agtcggcagg ggcggattcg ccgaagcggt tgatgccgac
     2821 gactgcgccg ttcaaaccga cgtatttgta ccagccgtcg gcgtgtccgg cttctacggc
     2881 gatgcgcggc agaccttcgg gcaggacggt ggcttgatag gcggcgtctt ggcggtcgaa
     2941 tacgttggtg gacggcatgg aaacgacgcg tacggcgatg ttttgcgcgg cgagggcttt
     3001 ttgcgcttcc aaagccagct cgacttcgga accggtggca atgatgacgg cttgcgcgtt
     3061 gccttgggct tcgctgatga tgtagccgcc gcgtttgatg tcgttcagtt gttgttcgct
     3121 gcgcgcttgg aatttcaggt tttgacggct gaaaatcagg caggacggat gatcggcggc
     3181 tttgacggct tctgcccaag ccaccagaga ttcggcggtg tcgcacggac gccatacgtc
     3241 catgttcgga atcaggcgca gggtggcggt ttgctcaatc ggctggtggg tcgggccgtc
     3301 ttcgcccaaa ccgatggaat cgtgggtgaa cacaaataca gggttgattt tcatcagtgc
     3361 cgccatgcgc aaggcgttgc gttcgtattc gctaaacatc aggaaggtcg cgccaaaggg
     3421 ttttacgccg ccgtgcaggg caagaccgtt cataatcgca cccataccga actcgcgcac
     3481 gccgtagtgg atgtagttgc cgcctttgtc gcgggtaacg gagacgctgt ttgaccagtc
     3541 ggtcaggttg gacggggtca ggtcggccga accgcccacc aattcaggca actcttttgc
     3601 caagatttcg atgctgtttt ggctggcttt gcgggtagcg atggtttcgg ctttggcgca
     3661 cacttctttc aatgccgtct gaacgtaccc gtcaaagttt tccggcagct ttttatccat
     3721 acggcgcaca aattctgcgg cttcggcagg atatttggct tgatattgcg cgaacagttc
     3781 gttccagtcg gcttccagtt tcgcgccttg ttctttggca ttccacgcat cgtaaatttc
     3841 ttgcgggatt tcaaaggcgg ggtaagtcca gcccaaatgt ttgcgcgtgg cttcgatttc
     3901 gtccgcgccc aaaggtgcgc cgtgggtttt gtggctgcct tctttgttgg cactgccttt
     3961 gccgattaag gttttgcagc agatgatgga cggtttgccg gtttcggcac gtgcggcttc
     4021 gatggcggct tgaatggcgg cggtgtcatg accgtttacg ttaggaacga cgtgccagcc
     4081 gtagctttca aagcgttgcg ggatgttttc ggtaaaccag ccgtccactt taccatcaat
     4141 ggaaatattg ttatcgtcat acaaaacaat cagtttgccc aagcctaaag tgccggcgag
     4201 tgagcaggct tcgtgcgata cgccttccat caggcagccg tcgcccatga agacgtaggt
     4261 gtaatgatcg acgatgttca aaccgtcttt attaaattcg gcggcaagga ttttttctgc
     4321 caatgccata cccaccgcgt tggcaatccc ttgccccaac gggccggtcg tggtttccac
     4381 gccgtcggtg tagccgtatt cgggatggcc gggggttttg ctgtgcagtt ggcggaagtt
     4441 tttcaagtct tcaatgctta ggttgtagcc ggtcaggtgc agcaggctgt acaacagcat
     4501 agacgcgtgg ccgttggaga ggacgaagcg gtcgcggttg tagaatttgg ggttggcggg
     4561 attgtgattg aggaatttcg tccacaatgt ttccgccatt tccgccatac ccataggcgc
     4621 gccggggtgg ccggaattgg ctttttgaac ggcatcggcc gagaggaagc ggattgcgtt
     4681 tgccagttga gacattttgt attttccttg ctggtgtttc ggataagtgg ataatcggaa
     4741 ggcgttgatt atcgcccgat tcgcttatgc tttcaagaaa agggcggacg cgtgggaagg
     4801 cggcggcagg ggacaggcgg ggaaggattt tcgatttgcg ggcgaagcct gccattattc
     4861 cttttgaaat aaaatgttat agattgtgtg ccggattgtc gatagcgttt gtttatgagc
     4921 ttgcgccgtc ggttctgccg atatgggggt gtcggttttt ttagtctttt ttttaaccgt
     4981 attcggattt tgttcgggcg gtaaggtaaa atccaggcgt tttgatgcgg atgggaaatg
     5041 tatccgcccc atacatccgg acggcgcata aagttgtaca atagcggaaa tatattttgg
     5101 gaaaaacgga ttttctcaaa acttgaaaca caacgcctta aaaaacaaga aaaatgacgt
     5161 ctgaaaagca aacggcggcg gtaacgggca aaccatcaat caaaaatcta aggaatgcag
     5221 aatgaccacg gaaaaccaag ccggcagtcc ggcatccgga atcggcacgt ccgaacaaac
     5281 aaaagccgcg ccgaaagcaa aaaaaacatt cgatccgcga gccagcgtga ttcaaatcca
     5341 tcccgaaggc gaacgcatcc atccgaaaaa ggcggaagga cggttcgcca aactgcgtat
     5401 cgccgccgta ttggcgacgc agtttgtgtt ttacgtcatt ccgtggttca actggagcgg
     5461 caggcaggcc gtcgttttca atatccccga acggcatttc ttcattttcg gattgtcgtt
     5521 gggggtgggc gatttgattt accttgcctt gctgctgatg atttgcgcct tcgggctgtt
     5581 ttggtggacg acgattgccg ggcgactgtg gtgcggctat tcctgcccgc aaacggttta
     5641 caccgaaatt atgctgtgga tcgacaacct ggtcgaaggc gatagaaaca aacgcctgaa
     5701 gctggaaaaa tcgccgtgga atttcactaa aatccgcatc aaagccacca aatacctgtt
     5761 gattttcctt gtctgcgcgt ggacgggcat cacgtttgca ggctggtttg tccctatccg
     5821 ccagttcgtt cccgatttat tcactggagc agcaggcggc ggcgcgatgt ttgccacagc
     5881 gttttacggc tttatgacct tcttcttcgc acatattatg cgcgaaaaag tatgcctgca
     5941 tatgtgtccg tatgcacgtt tccaaagcgc gatgttcgac aaggacacgc tgattgtttc
     6001 ttatgacgcg gaacgcggcg aaccgcgcgg agcccgcaag aaaacggtca acaaggaaga
     6061 ggcgggtttg ggcgactgca tcaactgtgc gatgtgcgtc caagtctgcc ctgtcggcat
     6121 cgacatccgc aacggtctgc aataccaatg catcggctgc gccgcctgta tcgacgcgtg
     6181 cgatgagatt atggacaaaa tgggctatcc gcgcggatta atccgttata cgaccgaaag
     6241 cgcgctggaa cacgaatatt ctgaaaaaga cattaaaaaa cggctgctca gaccgcgcgt
     6301 ggcaggttac ggcgcggtgt tggcgttagt tatcattgcc ttcctggccg gtttgtccac
     6361 gcgcaaaatg gtcgaggtcg atattttgaa agaccgtggc gtactggtgc gcgaaaacgc
     6421 caaaggctgg ctggaaaacg catacagcct gcgtatcatc aacaaaagtg aaaaagaaca
     6481 gctcattacc gcaagtgtca aaggctttga cgaaatcgcc ctgaccgggc tgcccgaagg
     6541 cggtatcaag gttgccccgc gcgaaacggt aacccttccc gtccaagtgt ccaccattcc
     6601 ggaatacgcg gacaaaggca gccaccctat cgaatttacc ttccaatacc gcgaaagcgg
     6661 cgcgcccgac ggcaagccgg tcgtcttgga agaagatgca acctttatcg gagaataacc
     6721 gtgtctcaaa acactccaat caaaccttgg tacaaacacg tctggccgtg gatcttgatg
     6781 gcggggccga tttttgtcgt catcgccagc gtcgctatgt tttttgtcgc acagcagcac
     6841 gcgacagatt tggttacgga cgattattat aaagacggca aacatatcga catccagctt
     6901 catcgggatg aagaagccgt cagacggcat atcggggtgc aggttctcat ttcccccgat
     6961 atgaatgcgg caaaagtgtt tgtcggcggc gagtttgacg gcaaacagcc tttgaacctg
     7021 ctgctgatgc acccgacccg caaggcggac gatcaaaccg tcgccctcaa gcccgtcggc
     7081 agcgcgcaga acggcagggc ggaatatgag gcggtgttca aaaccctttc gccgaccaac
     7141 cactggtatg tgcgcgtgga ggacgcggca ggcgtgtggc gcgtcgagaa caaatggatt
     7201 accagccaag gcaatgcggt ggatctgacc ccgatggata aacttttcaa taatgccgga
     7261 agcaaataaa aaagccgtgt tttcattgca ttggtttgcc agatgcaatg tcgggcaggc
     7321 attgtttgat tattcggcgt gatggtctga tttatcggac gaaaataaat gtatctcatc
     7381 ccgtttattt tttaacacta ttccagaacg cagcctgaaa aatgccgtct gaagggcttc
     7441 agacggcatt tgtgttgtat gcccgacaca atgcggaagg cgttttgttt acataaaaac
     7501 gtttttattt ctctccgttt tttcgacact attccgaaac gcagcctgaa aaatgccgtc
     7561 tgaaagccct tcagacggca ttttgtttgc aaatcaaatc ctacctgatg tcagcgtccg
     7621 agcgttgcaa caggttcgct ccccgtcagc agatattgta tcgtctcgcg gacggtgcgg
     7681 atggcattac cgaagggcag cgggtcttta cctctgaaga acaggccttt atctacttct
     7741 ccacgaaatg cggcagcaag ctggatatca atacagaatt gccctgcttt ggaaagcccg
     7801 tcgcgcaggc cgcagctagt caggcagttt aaaccttggg tacagcggcg cgggtcggct
     7861 ttggcgtttg tctgaagttt gctttcacgc ttgatgtagc tgtctaggaa tttggtgcgg
     7921 acaccgcgcg ccggcaaacc ggcaacagac ataaattcga ctactttttc agtttccgca
     7981 ccggcgagcg tttttttgaa gttaaggtgt gcatctcctt cttcggtaac ggcaaaagcc
     8041 gtaccgattt gaacggcgga tgctccccag ttctttaggg cggttttgac tttttcaaaa
     8101 tttgccatgc ctcccgcaag aataagcggg attttttcgc tttccagccc taaacttttg
     8161 aaaacttcaa acgtttcctc aatcacgcgt ttgaagtcga acttggcatc gtttacgcct
     8221 tcaacggttg atgcacccaa atgtccggcc gcgtgggcag gatgttcgac tacaatcgca
     8281 tcgggcaata tgcctttttt catccaacgt ttcaagacga tattaatacc gcgcgattcg
     8341 gacagaatcg gcagcagcgc gacatattta tgatagccct cggtcatttc cggcaggtct
     8401 aaaggcagac cggcacccat tacaaccgca tccgcccctg attcgcaagc ctggcggaca
     8461 tatgcggcgt ggtctttgac cgccttcatc acgttgaccg caatcagccc ttttccctct
     8521 gaagcacttt tggctttttg gatttccctg tccaatgcgg tacagttcaa agacgtatat
     8581 ttctcttcac tcggattgat ttgggattcg gcgagtaggt cttcgtgaag gtggcgcaaa
     8641 tccacactgg caatcgttcc gataccgttt tcacgtgcca ccgcgctgga taaacccgat
     8701 gcggaaacac cgacccccat accgccttgc acgatggggg taagggattt tccacgaata
     8761 accaaagggt caaaaatatt ttgcatcagt ttctccgagt atacgaatca gtcaggctca
     8821 tgaaaaatgg ttctaactgt tttagaacta ttgctcaaat ttggtattat actctaaata
     8881 tcgcggcggt aaacaagaaa gcagccggag cttcgggcat attctgttac gcctgtttca
     8941 atcgggagag aaaatattat agtgaattaa caaaaatcag gacaaggcga cgaagccgca
     9001 gacagtacaa atagtacgga aaggcgaggc aacgccgtac tggtttaaag ttaatccact
     9061 ataaatttga tgatggcata aaaaagccct agtcagttgc tgtcaaaggg gattgttaag
     9121 aaaagtaatg ccacgttgat ggggtgcaat atatcaggcg tttcaatcgg gtaaaatgtt
     9181 ggacggacac cgcatccggt tacgtgatat cggtttgcac ggcaaaggtt tgaatatgat
     9241 tcgattacgt ctgagagtct gatgaagggg gtaggtaaag atatggtaat gaattgccga
     9301 ggcaataaaa atccccgagg gattatttcg ggggatttgg aatttggcac gcccacgggg
     9361 aatcgaaccc cggttaccgc cgtgaaaggg cgatgtccta accgctagac gatgggcgca
     9421 tattgtattt tcttactggc gcacccggag cgattcgaac gcccgaccct ctggttcgta
     9481 gccagatact ctatccaact gagctacggg tgcactgcaa gaaagatttg aattttaggg
     9541 taatcatcgg attctgtcaa ttgttttgtt ggatgaaatg ccttaaaagt gtaaaatatc
     9601 ggtttgttgt ttgaatttaa agagaataaa atgattgttg cttcccgctg gcccttgctg
     9661 gaaaaggtat ttttacggtt tggaatgcct gttgccgtgg ttgatttgga atcgacgggc
     9721 ggcaatctgt atgaagacag ggtaaccgaa gtggctttgg tcaagtttga gcagggaagg
     9781 gtggtgaggc atgagtggtt ggttaatcct caaaaaccga ttccgcagtt tgttgcgggg
     9841 ctgacgggga tttcagacgg catggttgcc gacgcacctg tttttgcaga gattgccggc
     9901 gagttgtttt cggtattgaa gggttgtgtg ctggttgcac ataacagccg tttcgactat
     9961 acgtttttaa agcatgagtt tcatcgtgcg ggtatcggat tttcatcgcc tgctttgtgc
    10021 agtgtgcaac tgtcccggtg tctgtatccg caattttaca agcacagctt ggacagtatc
    10081 atcgaaaggt tggggattgt tgtggaagac aggcatcgtg cgatggcgga tgtatcggca
    10141 ttgtgcgatt atttggaata cagtctgtcg gaacacgggg tcgaggcatg gatcaggcag
    10201 tgtttccgtt tgatgaatcc gaaaccgctg cctgccgcgc tgcccgaacg gttgagggaa
    10261 cagttgtacg gtttgcctga cagtatgggg gtgctggctt gtttcgacgg aggagggaaa
    10321 gtaaattaca tcggtacgtt tgaacgggta tatagcgagg tttcggcttt attggattcc
    10381 ggaaaagcct cgtttgattg gtgcaatacg gaggcagtcc gtttttttcc cgcattgggc
    10441 aggctgcatg catataagat taaagcggaa ttggtcaggc gttatcattc gggttgttat
    10501 gtgtctgcca aaaatctgct gaaaacattt acgaccgtca ggtttgaaaa aggttcagac
    10561 ggcatgttga atgcgaaaac aacggctttg aaaaacggtg tgacggataa tccgcctacc
    10621 ggattatttg ccaataaaaa ggcagcgaga cgggctttgt cgtcgtgggc ggaaacatac
    10681 ggattgtgtc ctgccgcagc cggtatcctg tcggacggtt atacagagga tgagccttgt
    10741 cccgtatatg tttcaggccg atgcgataag gcgtgcggcc gttctgacga acaggttttg
    10801 gcgtttgcac acaagttgcc tattttggat tggggaaaaa tgcatgaggt agaaattacc
    10861 gaaactgatc ctttaacggg ggaaaaagtt gttctgcatg gaatgggcgg cgcattggaa
    10921 atgggtgacg ggctttggta tttcgataaa gatttaccgg atgcgtttaa ggcgaagttt
    10981 aaaacggaca ggaaaaatat taaggaaatc ggttaaatcg ataccggttg atgaaagccg
    11041 gtaagtatgt aaacagccgt tcgtattgat tgcctaatcg gtttgggttg gaatgtgttg
    11101 ccgtttgcag gtaatgtgtc cgaagatgcc gtctgaattt tttcagacgg catttttgcc
    11161 gacgcggttt tcgtgccgtg tcggtttgct cttccaaccg aaggcatcgg acaagcaggg
    11221 tggtttttag aagccgtttt ccatcatgat ttgaccagga atgcggttgc ggtgcatggc
    11281 gtaacccatg tcaagcagcg tttggaaggt gtctttgact atttccggat tgccgcaaat
    11341 cataaaccgt gtggattcgg gggtaaactt ggtatgcagc gcctgttcga tgctgttgtt
    11401 ttttaagagt tccggaatgc gttttccgcc taagcccgag gggttggcgg cacgggtggt
    11461 aacagggacg aaacggaacg agtgtccgta ttcgcctacc aggggatgtt cggccaatgc
    11521 ggcgagccgg tcgttgaaaa tcaattcttc gggaaaagat acggaatgta tcaggttgac
    11581 ggtatcgaaa cgctgccgga tttcgggttg ttcgagaatg gaaaggaagg gggcgatgcc
    11641 ggagccggtg cagagcatca ccaaatcctt gccgtcgggg aagcgttcgg gcaggaggaa
    11701 gccggtggca tttttatcga gcaggatggt atcgccctgt tgcattttgg cgaaacgggc
    11761 cgacatgggg ccgtcttgga tgagtacggc aaaatattcg agcgtgtcgg catattctgc
    11821 ggaaacgatg gagtaggcac gccaaataaa gccttcccct tcgtagaaac cgagtcggga
    11881 gaactgtccg gctttaaaac ggtaggattc gggacggctg atggcgaaag tgatgagttt
    11941 cggcgtgtgg tgtttgaccc acaaaatctt ttcttcagtg aattttgctt cgggcgaggc
    12001 tgccatgttg ttgtccttga gtgtgtttgt cgggattata gccaatttgg gtgttttttt
    12061 ccgcattgac ggtctgagac ctttgcaaaa ttcctttccc tcccgacagc cgaaacccaa
    12121 acacaggttt tcggctgttt tagccccaaa tacctcctaa ttttacccaa atatcccctt
    12181 aatcctcccc ggatacccga taatcaggca tccggaccgc cttttaggcg gcagcgggcg
    12241 cacttagcct gttggcggct ttcaacaggt tcaaacacat cgccttcagg tggctttgcg
    12301 cactcacttt gagcagc
//
LOCUS       DAXHMX010000052        12304 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHMX010000052 DAXHMX010000000
VERSION     DAXHMX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.052"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(12..848)
                     /locus_tag="ACFM4S_001036"
     CDS             complement(12..848)
                     /locus_tag="ACFM4S_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6050735.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLDLRLINGVEISVTWRGRTIHVVGLDFDERDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIRAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCRPVWEHFS
                     RH"
     gene            complement(1156..1557)
                     /locus_tag="ACFM4S_001037"
     CDS             complement(1156..1557)
                     /locus_tag="ACFM4S_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050736.1"
                     /translation="MAIYDLNEIVTSAALGMYKGKWQNMDFEQVLQDIVQETDFTYSW
                     EYGENWAIFCLNEKSVGMLCKQIPFAIIENEKLSQKIYQEYPWILTQQVYHFDTDKIL
                     LNKYVCKLTIGIMSAISQEPISLQDLYVDTV"
     gene            complement(1662..1865)
                     /locus_tag="ACFM4S_001038"
     CDS             complement(1662..1865)
                     /locus_tag="ACFM4S_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC6050737.1"
                     /translation="MDTTLKNKAEAILGEPLLDEPIRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(1843..2076)
                     /locus_tag="ACFM4S_001039"
     CDS             complement(1843..2076)
                     /locus_tag="ACFM4S_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6050738.1"
                     /translation="MEAIVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEE"
     gene            2614..6048
                     /gene="dnaE"
                     /locus_tag="ACFM4S_001040"
     CDS             2614..6048
                     /gene="dnaE"
                     /locus_tag="ACFM4S_001040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6050739.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLVAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQMARERYARSLSLALAPHHDIGKLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            6091..6345
                     /locus_tag="ACFM4S_001041"
                     /pseudo
     CDS             6091..6345
                     /locus_tag="ACFM4S_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6482..7414)
                     /locus_tag="ACFM4S_001042"
     CDS             complement(6482..7414)
                     /locus_tag="ACFM4S_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6050740.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMPNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFSAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSGCKRREERDMAGLSERMAAELDRHG
                     AAVFFREYPNADHGNVSFYSLTDCVEYLRKAWQR"
     gene            complement(7484..9640)
                     /locus_tag="ACFM4S_001043"
     CDS             complement(7484..9640)
                     /locus_tag="ACFM4S_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC6050741.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVCKRPAKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRHETFYAAADWDINPDTVLGAGYLYQQRHLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRDADSNYAFAGSKLGMKT
                     PAGRPGCNTADDKACAVGLGTEIKQKALAFDASYSRPFRLGNTANEFVIGADYNRFRS
                     TNEQGRTTLYARGGLALNEFRSIPQVDLIANARKGVRGYSHTVATENLDEFGIYGKST
                     FHPADGLSLIGGGRLGHYKIESGEGKTLHKASKTKFTGYAGAVYDLNDNNSLYLSLSQ
                     LYTPQTNLDADGKLLKPRQGNQFEVGYKGSYMDDRLNARVSFYRMKDKNAAAPLNPNN
                     KKTRYAALGKRVMEGVETEISGAVTPKWQIHAGYSYLHSQIKTASNSRDDGIFLLMPK
                     HSANLWTTYQVTPELTIGGGVNAMSGITSSAGMHAGGYATFDAMAAYRFTPKLKLQIN
                     ADNIFNRHYYARVGGANTFNIPGSERSLTANLRYSF"
     gene            9800..10459
                     /locus_tag="ACFM4S_001044"
     CDS             9800..10459
                     /locus_tag="ACFM4S_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC6050742.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRDLLEMPSETAIADITRTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGFDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(10546..11514)
                     /gene="ispH"
                     /locus_tag="ACFM4S_001045"
                     /gene_synonym="lytB"
     CDS             complement(10546..11514)
                     /gene="ispH"
                     /locus_tag="ACFM4S_001045"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6050743.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(11544..12038)
                     /gene="lspA"
                     /locus_tag="ACFM4S_001046"
     CDS             complement(11544..12038)
                     /gene="lspA"
                     /locus_tag="ACFM4S_001046"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6050744.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     GKKT"
BASE COUNT         2791 a         3426 c         3256 g         2831 t
ORIGIN      
        1 tttatccgca ttcaatgccg ggaaaaatgt tcccacaccg gcctgcagtt ttccggcagc
       61 tcgggacacg cgccgaggat accgccgctg aaatcgttta agcggtggaa atcgctgccc
      121 gcgcttgcca acaatccgaa gcgttctgcc aaaagcgcgt agttgaggcg gtcgtttttg
      181 caacagttgc cgctgtggac ttcgatgcct gcgccgccga ggtttttaaa ttcttcaaac
      241 agattgcgct tggcggtggc ggacaaatcg tagcgcatcg gatgggcgat gaccgccagc
      301 ccgcccgcgc cgttgacggc ggagacgcag tcttccagcg tcgcccattc gtgtcggacg
      361 gcgcaggatt tgccgtcgcc caagtatttg gtgaacgcct gctgcttgtt tttgacatgt
      421 cccgcccgga tgaggaactc ggcgacgtgg gtgcggctga ccatttcttt gtttgccgcc
      481 agcgcgagcg cgccgtcgta tgcgccgccg atgcctttct tttcgagctt ggcggcgatg
      541 gcttcgaggc gtttcagacg gccttgccgc acttgcgcca acaggttttg caggttttcg
      601 tcccgttcgt cgaaatccaa accgacaacg tgtatggtgc gtccgcgcca agtgacggag
      661 atttccacgc cgttaatcag gcgcaaatcg agcctgtcgg cttcggcacg cgcttcggcg
      721 atgccgccgg tgtggtcgtg gtcggtcaac gccagcagcg tgcagccgtt ttgatgcgcg
      781 aggcggacga cttcggcggg ggaaagcata ccgtcggaaa cggtggaatg gcagtgcagg
      841 tctatcatgg ggtgttatat gtgtggtgaa tgaaggtcgg ggggcaatat tgtcgggcgg
      901 tgtggcggcg gcggtcattt taaaaaacag gtgtatggca aatgcaaagg aaaagccgct
      961 atgccgtcat tcccaacccc cgaatgtgcg gatagagcca gtggggatgg gtacattatt
     1021 tgtttgcatt agttagttat cctgctgtat ccattaaatc atatgcttca attaattgtt
     1081 gttctagctc ttgtaccaat tttggattgc gaattcctaa ctcaatttca aacagcgagt
     1141 tctcactatg tacatctaca cagtatctac ataaagatct tgtaaagata taggttcttg
     1201 gctgatggca ctcattattc caatagttaa tttgcataca tatttgttta ataaaatttt
     1261 atctgtatca aaatgataaa cttgttgagt tagtatccaa ggatattctt ggtatatttt
     1321 ctgtgataat ttttcattct ctataatcgc gaaaggtatc tgtttacata acataccaac
     1381 agacttttcg tttagacaga aaatagccca attttcccca tattcccaag agtatgtaaa
     1441 atctgtttcc tgaacaatat cttgtaaaac ttgctcaaaa tccatgtttt gccatttacc
     1501 tttatacata cctaatgcgg cactggttac tatttcgttt aagtcataaa tagccattta
     1561 tgttccttgc taatttccag gtgagacatg tataggactg agaccagtat gcccattagg
     1621 tttccctcta tacacattaa tagtagatgt tggttccgtt ttcagacggc atcgtccgac
     1681 caacccgctt cttttaattt ccgccgttgc gcgtcgtagc gggccttatc cgcccaataa
     1741 atcgtctgaa tgcacgcctc gccgcagtcg ctgccgcagc attcccacga ctcggggcgg
     1801 ataggttcgt ctaaaagcgg ttcgcccaag atggcttcgg ctttattctt cagggtcgta
     1861 tccatcggca atttccaagc gtgcgccgtc aaactcgatg acttcgccgc cgcgtatttt
     1921 ggcggtttta cgggtttcgg tttcgccgtt gcgcgacacc aacccttcgg caatcaacgc
     1981 cttcgcctgt ccgccgcttt cggcaagtcc ggccaatttc aacaggtcgc acaaggcgat
     2041 gtattcgttg tcttcgagat agacgatggc ttccatgatg tccccttgca gaaagagacc
     2101 gttattgtag cacctgccgc cgccgtaccc aaaattaccg caaaaccagc gatgtatccg
     2161 caccgcctgt tccgtaaaag taaaaatgcc gtctgaaacc ccgtatgccg caatccgttc
     2221 aaagaaatcc tgcccaacgg cagactgcaa atcctgttcc ccgacgaatc cgcattgacg
     2281 ctgatgcaca tcctcaaaca cgaactgccc gatacaccgg caatcggcat caaaacgaaa
     2341 tcaaaaacct gtttgaacgt aaaattaatt taactgcttg ccatccgtcc gaataaggta
     2401 tatagttctt tataactagt ttgatagttc tttatatcta tcaatactcc ttgggaagcc
     2461 tccaccacac ggtaggaggc atttttttgc cgtcgtaaaa gctcaaaaac atttgcaggt
     2521 catgccgtct gaatccgaaa cagcattacc tacaccgcca tctaaagaca accctgctac
     2581 aatacgcctt ttattgttca cgccgatttt gccatgaccg agccgaccta catccccttg
     2641 cgcctgcata ccgagttctc aattaccgac ggtatggtgc ggattaaaaa actggttgcc
     2701 aaagcgcagg aatacggttt gcctgctttg ggcatcagcg atttgatgaa cgaattcggc
     2761 ttggtgaaat tctataaagc ctgccgcggc gcagggatta aacccatcgg cgcggcggac
     2821 gtgtggatag gcaatccgaa tgcgcccgac aagccgtttc gcgccatgct gattatccgc
     2881 aacgatgcgg gctatctgcg cttgagcgag cttctgacgg cggcttatgt cggcaaagac
     2941 cgcaatatcc atcatgcgga actcaatccc gaatggctgg aaaacggcga caacagcggc
     3001 ttgatttgtt tgagcggcgc acattacggc gaagtgggcg tgaatctgtt gaacggcaat
     3061 gaagacgcgg cgcgtgcggc ggcgttgaaa tatgcggcat ggtttcccga tgcgttttat
     3121 ttagagctgc aacgcctgcc cgaacgcccc gaatgggagg cttgcgtttc cggcagcgtg
     3181 aagctggcgg aggaactggg tttgccggtg gtggcgaccc acccgacgca gtttatgagc
     3241 agcgatgatt tcaacgcgca cgaggcacgg gtgtgtattg cgggcggctg ggtgctgacg
     3301 gacaaaaaac gtccgcgcga tttcacaccg ggccagtttt tcattccgcc ggaaacgatg
     3361 ctggagcgtt tcgccgattt gcccgaggcc ttggaaaaca cggtagaaat tgccaaacgc
     3421 tgcaatctgc acatcacatt gggcaaaaac ttcctgcccc ttttccccac gcccgacggt
     3481 ttatcgcgcg atgactatct catcaaactc tcaaacaagg gcttgcagga acgcatggtt
     3541 cagctttatc ccgacgaggc ggagcgtgcg gcaaaaatgc cggaatatca ggaacgtttg
     3601 gattttgagc tgaacatcat catccagatg aaattccccg gctatttcct tatcgtacaa
     3661 gactttatca actgggcgaa aacgcacggc tgtccggtcg ggccgggacg cggttcgggc
     3721 gcgggttcgc tggtggcgta ttcgctgaaa attaccgacc tcgacccgct gaaatacgcg
     3781 ctgctgtttg aacgtttctt aaaccccgaa cgcgtctcca tgcccgactt cgacgttgac
     3841 ttctgtcagg caaaccgcgg ccgcgtgatc gaatatgtgc gcgataaata cggcgctcag
     3901 gcggtcagcc agattgttac cttcggcacg atgtcgtcca aagcggtcat ccgcgacgtg
     3961 gggcgcgtgt tggagctgcc gtttatgctg tgcgacaaac tgtccaagct gattccgttg
     4021 gaagccaaca aacctctggg tttggacgac gcgatgaagg cggagccgca gattcaggaa
     4081 ttaatcgaag cggaagaagc ggacgaactg attacgctgg cgaaaaagct ggaagacctg
     4141 acgcgcggtt tgggtatgca cgcaggcggc gtgttgattg cgccgggcaa gatttccgat
     4201 tacagccccg tgtatcaggc ggatgaatcc gcctctcccg tatccatgta cgacaagggc
     4261 gacgtggaag acgtgggttt ggtgaaattc gactttttag gtctgcgtaa cctgaccatt
     4321 atcgaaatgg cgcagaacaa catcaaaaac accaccggcg acatcatcga tgtcggcaaa
     4381 attccgctcg acgaccaagc cgcctaccaa atcttccgag atgcgaacac caccgccgtg
     4441 ttccagttcg agtcgaccgg catgaaaaaa atgctgaaaa cggcgcacac gaccaagttt
     4501 gaagaactga ttgccttcgt atcgctctac cgccccggcc cgatggacaa catccccgac
     4561 ttcgtcgcgc gcatgaaagg ccaggaattc caatacatcc atccgctgct cgaaggcatc
     4621 ctcgcgccga cctacgggat tatggtgtat caggaacaag tgatgcaggc ggcgcaaatt
     4681 atcggcggct actcgctcgg cggcgcggac ctgctgcgcc gcgcgatggg taagaaaaag
     4741 cccgaagaaa tggtgaaaca ccgcgaaatc tttgccgaag gcgcggcaaa acaaggcatt
     4801 tcgcgcgaaa aatccgacga aatcttcaac tacatggaaa aattcgccgg ctacggtttc
     4861 aacaaatccc acgccgccgc ctacgccctg atttcctacc agaccgcatg gcttaaagcc
     4921 cactatcccg ccgaatttat ggcggcgacc atgtcgtccg aattggacaa caccgaccag
     4981 ctcaagcatt tctacgacga ctgccgcgcc aacggcatcg agttcctgcc gcccgacatc
     5041 aacgaatccg actaccgctt cacgccgtat ccggacatga aaatccgcta cgcgctcggc
     5101 gcgattaaag gcacgggcga agccgccgtc gaatccatca tcgccgcgcg gcaaagcggc
     5161 ggcaagttta ccggcctctt ggacttctgc gagcgcgtcg gcaaagaaca catgaaccgc
     5221 cgcacccttg aagccctgat acgcggtggc gcgttcgaca gcatcgaacc caaccgcgcc
     5281 atgctcttgg cgaacatcga cctcgctatg gacaacgccg accaaaaagc cgccaacgcc
     5341 aatcagggcg ggcttttcga catgatggaa gacgccatcg aaccggtgcg gctcatcgac
     5401 gcgccgatgt ggagcgaatc ggaaaaactc gccgaagaaa aaaccgtcat cggcttttac
     5461 ctgtccggcc acccgttcgg cccgtatgcc caagaagtcc gccaaatcgc cccgcagaaa
     5521 ctgtcgaaac tcaaaccgca agacagcgtg cgcctcgccg gattcgttac cgccgtacgc
     5581 accatgatgg gcaagcgcgg caaaatcgcc ttcgtcagcc ttgaagattt gagcggacag
     5641 gttgaaatca tggtcggcgg tcagacgttg gaaaactgcg ccgactgcct caaagccgac
     5701 caagtgctga ttatcgaatc caaagtcagc cgcgacgact acggcggcgg cgacgggctg
     5761 cgtattctgg caaaccaagt catgaccctg caaatggcgc gcgaacgcta cgcccgaagc
     5821 ctcagcctcg ccctcgcccc gcatcacgac atcggcaaac tggtacaact gctcgccgcc
     5881 caccaactgc ccgacacgcc gcgcatcccg ctgcaactgt cgtatgccaa cgaaaaagcg
     5941 tcgggcaggc ttcaagtgcc gccgaaatgg acggttacgc cgagttccgc attgttcggc
     6001 gaactggaaa cattgctcgg cagccggtcg gtgcgcgtca actggtaacc caaaatataa
     6061 atgccgtctg aagcccaaaa accggtttca ttcgtacttt attcgaatga ttgaataaaa
     6121 gtaactgcca agaaaaacgt attttttggt tatttcgcca gtctaaatag agcaaccggg
     6181 acgattgata tccgtgtgca tgacacagac agcaccaaag ggaaaaacgg cattttccaa
     6241 agtatcggta tcaaaaccgc cctttcactc caaaaatacc aaatcgacaa accgggcaaa
     6301 gaaatcagac cgtgccgcct gaaaaaacgc ccacccgtcc gttaaattaa cctatccctg
     6361 tttcagacgg cctgaagcag ggatttttat atcaaaataa aatgagaaag ggagcaataa
     6421 cccttaggta gctcttgtta ttttccgatg caaaacaaag cagtcatata tttaattccc
     6481 cctacctctg ccaagccttc ctcaaatatt cgacgcaatc ggtcagcgag tagaacgaga
     6541 cattgccgtg gtcggcattc ggatattccc ggaaaaagac ggcggccccg tgcctgtcca
     6601 actctgccgc catgcgttcg gacagtcctg ccatatcgcg ttcttccctg cgtttgcaac
     6661 cgctaccccg ttcgagcgcg ccgatgttga ggcagacatc gatgccgttt agccggtttt
     6721 cagacggcat aaaatcgagt atccgcctgt tgtgccacca aatcgatggg gatacgagcc
     6781 aatgccgtct gaaacggcgg tgggaaagca gggaatacag tccgaacagt gcgccgaacg
     6841 agtgtccgaa tacggcggtt tcattgcggt tgagggtgta gcggctttct aaaaaggcgg
     6901 tcagctcgct gtcgataaag gcggagaagc ggtctgcctg tccgaactgc tgccgttcgt
     6961 ctgctgtggc gttgtctcca agcggcggcg tgtagtcggc ggcacgttgt gccaaatcgc
     7021 gcacactgcc tgtcgtgtag ccgataccga caatcaggca gggggcgttg cttcgggtaa
     7081 cggggttggg catcagcgac tgcatgatgt tgaaaagtgc ggggaaaaag gcttcgccgt
     7141 ccaaaacgaa gaggacggga tagccttcag acggcatttc gccgagtgtc gccgtctgaa
     7201 tccgatagat tcgccccgtg caggtggatt tgatttcggt ttcaaaggct tggggcagta
     7261 tggcaggttg gaatgtgtcg gtcggtatgg gtttcatgat gttcggcttg tgggtcagac
     7321 tggcggcaag cggtaaaacc gaaccggcaa agcggtctgc ctgccggttc ggtctgtttt
     7381 ccttcgcaat gccatactcc agttgtgaga gcatagggta tgcagcgcag cttgttgtgg
     7441 tttgatgatg ctgcggctgc cgtttcagac ggcatattgg tctttaaaaa ctgtaacgca
     7501 ggtttgccgt caggctgcgc tccgaaccgg gaatgttaaa ggtgttcgcg ccgccgacgc
     7561 gggcgtagta atggcggttg aagatgttgt cggcgttgat ttgcagcttc agcttgggcg
     7621 tgaagcggta tgccgccatc gcatcgaacg tggcataacc gcctgcatgc atccctgcag
     7681 atgaagtaat gccgctcatc gcgttcactc cgccgccgat ggtcagctcg ggcgtaactt
     7741 ggtaagtcgt ccacaggttt gcgctgtgtt tgggcatcaa taggaagatg ccgtcgtcgc
     7801 gtgaattgga ggcggttttg atttggctgt gcagatagct gtaacctgca tggatttgcc
     7861 atttcggtgt aaccgcgccg ctgatttcgg tctcaacgcc ttccatcacg cgtttgccca
     7921 atgcggcgta acgggttttt ttgttgttcg ggttcaacgg tgcggcggcg tttttgtctt
     7981 tcatgcggta gaacgaaact cgggcattga ggcggtcgtc catgtagctg cctttgtaac
     8041 cgacttcaaa ctggttgcct tggcgcggtt tgagcagctt gccgtcggca tcgaggttgg
     8101 tttgcggtgt gtagagctgg gacaggctca ggtagaggct gttgttgtcg ttcaagtcgt
     8161 aaaccgcgcc tgcgtagccg gtgaacttgg ttttgctggc tttgtgcagg gttttgcctt
     8221 cgcccgactc gattttatag tgtcccaaac gtccgccgcc gataagcgac agcccgtcgg
     8281 caggatggaa ggtggatttg ccgtaaatgc cgaattcgtc gaggttttcg gtagcgacgg
     8341 tatggctgta accgcgcacg cctttgcgcg cgttggcaat caaatcaacc tgcggtatgc
     8401 tgcggaactc gtttaaagcc aggccgccgc gtgcatataa agtagtacgg ccttgttcgt
     8461 tggtgctgcg gaagcggttg taatcggcgc cgatgacaaa ttcgttggcc gtattgccca
     8521 agcggaaagg cctgctgtag ctggcgtcaa acgcgagggc tttttgtttg atttctgtac
     8581 ccaaacccac cgcgcaggct ttgtcgtcag ccgtattgca gcccgggcgg cctgccgggg
     8641 ttttcatgcc cagcttgctg ccggcaaagg catagttgga gtcggcatcg cggtcggaat
     8701 agcgcatacc gactttgccg tagccgccgt tgccgaagta atgtttcaaa tcggcgaaca
     8761 cgtcgtggct gtgcatttta aatttgttcc aatccgcgcc gacaaatacg tgttgcggca
     8821 gggacggtaa tttgttattg gcatcggctg gcaagccgtt gtacggcgcg aggtggcgtt
     8881 gctggtaaag atagcccgcg cccaaaaccg tatcggggtt gatgtcccaa tccgccgccg
     8941 cgtagaaggt ttcgtgccgg ttgtttttct cggcgggacg cggagacgcg ccgacggtct
     9001 gcgccatcac gcggccgcgc acgctgccgt ctgaattgag gctgcccgat acgtccgcct
     9061 cggctttata ttgtttgtgc gtaccgaacc ctgccgcagc atgaccttgg aacgctttgg
     9121 ccgggcgttt gcacaccaga tttacgatgc cgcccatctc gccgctgctg tcgaacaagc
     9181 cgctcggccc gcgcatcact tccacacggt cgaaggcaaa cagattgggc agcgtgccgt
     9241 tgatactctg catctgcgcg ggcaggccgt cgatgttgta ttcgctgtat tcgtaaccgc
     9301 gcgcgtaaac cgaagagcgt ccgtcgtcgt tgctcaacac gcgcaggccg ggcgttttgc
     9361 gtgccaactg gtcaaacgta tcaacattgc ggtctttgac ctgttgattg gtgatgatgc
     9421 tgacagattg cggaatttcg cgcaaagaag cggggatttt tgtgccgacg gtggcggcaa
     9481 acgagctgta atcgccgttt ttctcggtgg caatcgcgtt gtacgaacgc tgacccttaa
     9541 tatggacggt ttccaaatcc gccgtttgtg cggcaaaaac cgaagacgaa agtgctgcca
     9601 aaaccgtggc ggcggtcata ttgatgcgga aaactgacat aaactgtccc attcacataa
     9661 atgataatgg ttctatttta ataaagcaca acgcggcttg ttcggaaaaa catatcgcgc
     9721 agccgacaaa ttttgtcgaa aatgcgacac gtctgcgttt tccgcataaa atctgacttt
     9781 tcaccgtaaa caacctgcca tgaccacgcc caaactcatc atcttcgact gggacggcac
     9841 gcttgccgat acgacccaac ccatcatcga caccatgcgc cgcagcttcg ccgaatgcgg
     9901 ttttccgccg cccgaagcgg aacgcgtccg cagcctgatt ggctacagcc tgcccgaaat
     9961 catccgcgac ctgctcgaaa tgccgtctga aaccgccatt gccgacatca cacgcactta
    10021 ttccgcacat tacctcaatc ccaacaaccg caatatgacg ctctttcccg atgccctgcc
    10081 ctgtctggac aagctcaaag cacaaggata ctggcttgcc gtcgccacgg gcaaagggcg
    10141 ggcgggtttg gacaacgcca tcagccaaac cgccaccggc ggctattggc tcgccaccgc
    10201 ctgcgcgggg gaatatccct ccaaaccctc gcccgaaatg gtattcggaa tctgcggcga
    10261 actggggttc gacccgaaag aggcattggt cgtcggcgat acggcgcatg acctgcatat
    10321 ggcggcaaac gcaggcgcgg cggcagtcgg cgttgccacc ggcgcacatt cgcgcgaaca
    10381 gctccttagc gcaccgcatc tcgccgtatt ggacggtttg tccgaactgc ccggttttct
    10441 tgcacagcat tacgcctgat tggtttccgc atccggcact cgaaacaaat gccgtctgaa
    10501 ggttgttcag acggcatttg ttttgcccaa acattcaacg cttgctcaac gtttgcacaa
    10561 atcgggtttg gtttcgccct cgcggcgcaa ctctttgggc aggacgaaca cgatgctttc
    10621 ttccgcgccc tccccttcgc gtacggtttc atgcccccac ccgcgtatgg ttgccaatac
    10681 ttcctgcacc aacacttcgg gcgcggacgc gcctgccgtt acgccgactt tgttcttgcc
    10741 ctcaaaccac gcgcgttgca ggtagcctgc attgtccacc atatacgcat cggttccgat
    10801 gcctgccgcc acttcgcgca ggcggttgct gttggacgaa ttgggcgaac cgaccacaat
    10861 cacgatgtcg cactgttctg ccaactcttt gacggcggtt tgccggttgg tcgtcgcata
    10921 gcagatatct tccttgtgcg gattgcggat attggggaaa cgcgcgttca gcgcggcgat
    10981 gatgtctttg gtttcatcga ccgagagcgt ggtttggctg acataggcga gtttgtcggg
    11041 gtttctgact tcgagttttg ccacatctcc gaccgtttcg accaaaagca ttttgcccgg
    11101 cgcaagctgc cccatcgttc cttcaacctc gacgtgcccc ttatgcccga tcatgatgat
    11161 ttcacagtct tgggcatcca gtcgggcgac ttccttatgc actttcgtca ccagcgggca
    11221 agtcgcatca aacacgcgga aaccgcgctc cgccgcttct tgccgcaccg ccttcgatac
    11281 gccgtgtgcc gaataaacca gtgtcgcgcc cggcggcact tccgccaagt cttcaataaa
    11341 caccgcacct ttttcacgca ggttgtccac gacgaatttg ttgtgaacga cttcgtggcg
    11401 cacataaatc ggcgcgccga attcttccaa agcacgttcg acaatactga ttgcccgatc
    11461 cacgccggcg cagaagccgc gcggattggc aaggatgatg gttttctcgt tcataagttt
    11521 tgcattccgt gttcagacgg cattcacgtt tttttgccat ctttgcgatg gacgatattg
    11581 tcaagcactg ccaataccgc gccgacgcag ataaaactgt cggcaatatt aaaggcggga
    11641 taaaaccaat tttgccaata aaacaataag aaatcgacga catgaccgtg tatcaggcga
    11701 tcgatgacat tgcctaacgc accgccgata atcatcgccg cacccgtttt gccgagggct
    11761 gcaaactcat cgcgcaagat ggcgcgtacc aaatacgcgc tcaccgccac cgccagcacc
    11821 aaaaaaaagt atttttgcca gccgccctga tcggcaagga agctgaacgc cgcacccggg
    11881 ttgtacacca gcgtcagatc gaaaaaggaa ggaatgacat tgacgcgttc ccgatactga
    11941 aacgacgaca gcaccgccca cttcgaccac tggtccagca cgatggcggc aagtgccaat
    12001 acccaatagc gcgttttact tgaaacagat gaagacatat ttttcaacag ccggtaaaag
    12061 agtaccattt tacccgaaaa ccccctttcc tgtacccgaa acggcaaatg ccgtaatctt
    12121 aaaacccgtc attcccgaca acaccgtaat ctcgaaaccc gtcattcccg cgtaggcggg
    12181 aatccagacc tgtccgcaca gaaacttatc ggataaaaac agttgctcaa acccctcgtt
    12241 ctagattccc actttcgtgg gaatgacggt tcagttgcgt tccaacaaca ccgcaatctc
    12301 gaaa
//
LOCUS       DAXHMX010000053        12200 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHMX010000053 DAXHMX010000000
VERSION     DAXHMX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.053"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(170..1099)
                     /locus_tag="ACFM4S_001047"
     CDS             complement(170..1099)
                     /locus_tag="ACFM4S_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6050745.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1230..1979
                     /locus_tag="ACFM4S_001048"
     CDS             1230..1979
                     /locus_tag="ACFM4S_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6050746.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            2102..2683
                     /gene="petA"
                     /locus_tag="ACFM4S_001049"
     CDS             2102..2683
                     /gene="petA"
                     /locus_tag="ACFM4S_001049"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6050747.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2702..4051
                     /locus_tag="ACFM4S_001050"
     CDS             2702..4051
                     /locus_tag="ACFM4S_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6050748.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            4066..4854
                     /locus_tag="ACFM4S_001051"
     CDS             4066..4854
                     /locus_tag="ACFM4S_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6050749.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5193..6977
                     /gene="rmuC"
                     /locus_tag="ACFM4S_001052"
     CDS             5193..6977
                     /gene="rmuC"
                     /locus_tag="ACFM4S_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6050750.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQEQKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHIDLQEKAQGLAVENERLTTQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAAAVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYAAEDEAV"
     gene            complement(7184..7816)
                     /locus_tag="ACFM4S_001053"
     CDS             complement(7184..7816)
                     /locus_tag="ACFM4S_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6050751.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNHWLEEGETLKVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7907..8731
                     /locus_tag="ACFM4S_001054"
     CDS             7907..8731
                     /locus_tag="ACFM4S_001054"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6050752.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKDQEIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQTKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8800..9363
                     /locus_tag="ACFM4S_001055"
     CDS             8800..9363
                     /locus_tag="ACFM4S_001055"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6050753.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9445..9882
                     /locus_tag="ACFM4S_001056"
     CDS             9445..9882
                     /locus_tag="ACFM4S_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6050754.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9951..10220
                     /locus_tag="ACFM4S_001057"
     CDS             9951..10220
                     /locus_tag="ACFM4S_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6050755.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            10220..11995
                     /gene="ptsP"
                     /locus_tag="ACFM4S_001058"
     CDS             10220..11995
                     /gene="ptsP"
                     /locus_tag="ACFM4S_001058"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6050756.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIAKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
BASE COUNT         2990 a         3052 c         3416 g         2742 t
ORIGIN      
        1 ttgggcaaaa atcaaaaaca gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
       61 cgcagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt ttgttaatcc
      121 actatacatg aaaaaataga aaaactgccg ggtatggttg aataaggggt cagaccggtt
      181 ccaattcgct cagtccgggc aaatctgcaa aaccgcgttc gcgtatgatt tggcaaaaat
      241 tgttcagata gctcttgtcc gtatcttcgg tacggatggc ggcatacagt ttgctttgca
      301 atccgtcggc agtaatttgg cggtggacga catagccttt ttcaaggtag ggcatgactg
      361 tccaataggg aagggcggca atgccacgtc tgctggcaac cagttggata atggcgatgg
      421 tcagttcgct gtgtcggcgc ggcgggttga tgtttttcgg aatcaggatt tttttgggca
      481 aatccagcat ctcgtcggga acgggataag taatcagggt ttccccgata aagtcttccg
      541 ccgtccaaac gtttttggcg gcaagcggat ggtctggtgc gcaaatgccg accatttcgt
      601 aggcaaacag cggttggaaa gaaatatcgt tttgtttttc cgcttcggaa acaatggcaa
      661 ggtcggcacg gtgttgcagc agcagtccga cgggatccgc ttggaatccc gatacgatat
      721 ccaattcgac ttggggccac atcgggcgga attcgcccat ggcgggcatc agccagtcga
      781 aacaggtatg gcattcgacg gcaatccgca attctcctgc ctctccttct ataatgcgtg
      841 ccaaatcacg ttctgcatcg gtaacttgag gcaacaggtc gtgagcgagg cgtaacagcc
      901 tttctccaac cggggtaaag cgcaagggcg tggattttcg ttcgaacaac ggtgcgccgt
      961 agtggttttc aagcatacgg atttggtggg aaagtgcaga ttgggtaagg aaaacccgtt
     1021 tggcggcaag ggagacgctg ccggtttctt caagtgccag cagggtcttg aggtggcgca
     1081 gttcgatgat ggaatccatg aggtttcaga cggcatattg aaccggcgca tattaaaata
     1141 attcatatgc gggtgcaaat cggaaaaatg tgaattccag acattttgcg attagaatgc
     1201 ccgcttgttt aaagcgatta ggaaaaaaga tggtattgcg cagggatttt ctgacttggt
     1261 gtaatgaaac attgcagaca gcgttgttta aagattacgc ccctaacggt ttgcaggttg
     1321 aagggaggga atatatcggg aaaatcgtta cgtcggtaac ggcaagcagg gcagcgattg
     1381 attttgctgt ggagcagaag gcagatttgc ttttggtaca tcacggtatg ttctggaaaa
     1441 acgagttgcc gaccgttact ggttggaaaa aagaacggat tgccgcactg ttacggcacg
     1501 acatcaatat ggcaggctac catctgcccc tggatgcaca tcccacactg ggcaacaatg
     1561 cccaactcgc cgacagattg ggttttgcga cagaaaaacg gttcggcgaa caaaacctgc
     1621 tcaactcggg cagcctgaaa caagccaaga cactcggcgc attggcggcg catattgaaa
     1681 cagttttgca acgtaagcct gtcgtcatcg gcaaacccga acgcgaaatc cgacgggttg
     1741 catggtgcag cggcggggcg caggggtttt ttcagacggc aatagacgaa ggtgtcgatc
     1801 tgtatttgac gggggaaatc tctgaagctc aataccacct tgccaatgaa acgggtacgg
     1861 ctttcatttc ggcagggcat cacgcgacgg aacgttacgg cgtacgcgcg ctggcagaat
     1921 cggcggcaga ggttttcggg ttggaagtgt gccattttga cgaaaacaat ccggcttgag
     1981 tcttgagaaa tatcataaaa ctttacctta ttttaaataa tgctttgagt atcactgcaa
     2041 actgggtaaa attgcaacgt ttaatggctc ggtattccca aaatattagg acgactgaat
     2101 aatggataat caagaaatca acaacggccg ccgccgtttc ctgacactcg cgacctgcgg
     2161 cgcgggcgga gtggcagcat tgggtgtggc aacgccgttt gtggccagtt ttttcccttc
     2221 ggaaaaagcc aaggccgccg gtgctgccgt cgaggtggat gtcagtaaaa tcgaagcggg
     2281 tcagctgctg accgccgagt ggcaaggcaa accgatttgg gtgctcaacc gtacagatca
     2341 gcagcttaaa gacctgaaag gcctgaacgg cgaacttacc gatcccaatt ccgatgcgga
     2401 acagcagccg gagtatgcta aaaacgagac ccgttcgatt aagccgaaca tccttgtcgc
     2461 catcggtatc tgcacccatt tgggctgctc gcccaccttc cgtcccgaca ttgcccccgc
     2521 cgatttgggt gcagactgga aaggcggctt cttctgcccc tgccacggtt cgaaattcga
     2581 cttggccggc cgcgtatata aaggtgttcc tgccccgacc aacctggttg tcccgccata
     2641 taaatacttg agcgacacaa ctatcttggt gggcgaagac taagaataag gaacgataat
     2701 tatggcaaac caaaccaata gcaaagcaaa agcattgtta ggctggatgg acgctcgttt
     2761 ccccctgtct aaaatgtgga atgagcattt ggctcaatac tacgcaccga aaaactttaa
     2821 tttttggtac tacttcggct ctttggccct gcttgtcctc gtgattcaaa tcgtcagcgg
     2881 cattttcctg accatgaact acaaacccga cggcaacctg aacgcctacc atctgcctgc
     2941 tgcctttact gcagtcgagt acatcatgcg cgacgtgtcc ggcggttgga ttatccgcta
     3001 catgcattcc accggcgcat ctttcttctt cattgttgtt tatctgcaca tgttccgtgg
     3061 tttgatttac ggttcgtaca aaaaaccccg tgagttggtg tggattttcg gttctctgat
     3121 tttcttggca ttgatggcgg aagcctttat gggctacctg ctgccttggg gtcagatgtc
     3181 cttttggggg gcgcaggtaa ttattaacct gttctccgcc atccctgtta tcggtccgga
     3241 tttgtccact tggattcgcg gcgacttcaa cgtttccgac gttaccctga accgtttctt
     3301 tgccctgcac gttatcgcgg tacctctggt attgctcggc ttggttgtgg cgcacatcat
     3361 cgccctgcac gaagtgggtt ctaacaaccc tgacggcgtt gaaatcaaga aaaacaaaga
     3421 cgaaaacggt atcccgcgcg acggtatccc attccatcct tactataccg ttaaagacat
     3481 cttgggtgtg gttgtattcc tgattgtctt ctgtgccgta ttgttcttcg caccggaagg
     3541 cggcggctac ttcctcgaag cgccgaactt cgatgcggca aatgcgctga aaacaccccc
     3601 gcacattgcg ccggtatggt atttcacacc gttctacgcc attttgcgtg cgattccctc
     3661 gttcttggga acgcaggttt ggggtgtaat cggtatgggc gcggcggtcg tgttgattgc
     3721 cctgctgcct tggctggata ggggcgaggt gaaatccgtc cgttaccgcg gcccgatttt
     3781 caaaaccgca ttggtcttgt tcatcatttc ctttatcggt ttgggtattt tgggcgcgat
     3841 ggtggcaacc gatacgcgta ctttggtcgc gcgcatcctg tccttcgtct atttcgcatt
     3901 cttcctgggt atgccgttct ataccaagct ggataaaaac aaaccggttc ccgaacgcgt
     3961 aaccatgagc accgccaaac aaaaaatcat gttctttgtt tacgtcggca ttaccgccgt
     4021 gggtgcttac ctgtttgcaa ccaacatctg atgagggcag tcaaaatgaa acaagctatg
     4081 aaaaattggt ttgctgcctt gctgttggca gtacccatgg gtgcggcatt tgcttcgggc
     4141 ggtcatgcac actatgagaa agtcgatatc gatttgcgcg accaagtcag cctgcagcgc
     4201 ggcgcacaaa tctttaccaa ctattgcctg tcctgccact cggcaagcgg tatgcgtttc
     4261 aaccgtttga aagacatcgg tttgactgac gaagaaatca agaaaaacct gatgtttacc
     4321 accgataatg tcggcgatgt catgcattcg gcgatgaacc cgaaagatgc ggcaaaatgg
     4381 tttggtgctg ctccgcccga tttgacattg attgcgcgtt ccaaaggtgc agactacctt
     4441 tacgcttata tgcgcggctt ctataaagat ccgacccgtc cgagcggctg gaacaatact
     4501 gtattcgata aagtcggtat gccccacccg ttgtgggaac agcaaggcgt tcaagccgtt
     4561 gagttggatg ccaaaggtca gccggttatg gtaaaagacg aacacggcga gatgaagcct
     4621 aagctgtatt gggaatctac cggtttgcac agccgccgtc tgcctaacgg caaagtgatc
     4681 caaaaagagt acgacgcata tgtacgcgat ttggtcaatt accttgtgta catgggcgaa
     4741 cctgcacaac tgcaacgcaa acgtataggc tatgtcgtga tgattttcct atttgcggtt
     4801 atgctgcctt tggcgtattt cctgaaaaaa gaatattgga aagacgtaca ctaagcgttt
     4861 ggaacaaaag ggcaaatcct ttagggtttg cccttttttc attttgcctg ccgtttgaaa
     4921 agcctgaatc cgtatgccgt ctgaacatag ctgcaacatt tcagacggca tcattctaaa
     4981 aatgtcagac atcgggaact taatcagggt ttgtggcaga ttgatattga attaggaaaa
     5041 ggtctgaaac cgcaagcagc atggtaaggc gatgtcgaac aggtttaaag ccaatccgct
     5101 atattttttg gagctatgtc gaacaaacag gtagaatcgg aataagcggc ggttttgtcc
     5161 ggtgtgaaaa tgtttgaata ttaggatgat tgatggaact gatgactgtt ttgctgcctt
     5221 tggcggcgtt ggtgtcgggc gtgttgttta catggttgct gatgaagggc aggtttcagg
     5281 gcgagtttgc cgatttgaac gcgcaactgg cggaaaaggc ggcaagatgt gattttgtcg
     5341 aacaggcaca cgcagaaatt gcatcggaat tggccgtttt ggacgggaaa taccgacatt
     5401 tgcaggacga aaattatgct ttgggcaacc gtttttccgc agccgaaaag cagattgccc
     5461 atttgcagga aaaagaggcg gaatcggttc ggctgaagca gtcgtatatc gagttgcagg
     5521 aaaaggcgca gggtttggcg gttgaaaacg aacgtttggc aacccagctc ggacaggagc
     5581 agaaggcgtt tgccgatcaa tatgccttgg aacgccaaat ccgccaaaga atcgaaaccg
     5641 atttggaaga aagccgccaa actgtccgcg acgtgcaaaa cgacctttcc gatgtcggca
     5701 accgttttgc cgcagccgaa aaacagattg cccatttgca ggaaaaagag gcggaagcgg
     5761 agcgtttgag gcagtcgcat atcgatttgc aggaaaaggc acagggtttg gcggttgaaa
     5821 acgaacgttt gacaacgcaa atcgaacagg aacgccttgc ttctgaagag aaactgtcct
     5881 tactgggcga ggcgcgcaag agtttgagcg atcagtttca aaatcttgcc aacacgattt
     5941 tggaagaaaa aagccgccgt tttaccgagc agaaccgcga gcagcttcat caggttttga
     6001 acccgttaaa cgaacgcatc cacggtttcg gcgagttggt caggcaaacc tatgataaag
     6061 aatcgcgcga gcggctgact ttggaaaacg aattgaaacg gcttcaaggg ttgaacgcgc
     6121 agcttcacag cgaggcaaag gctttaacca acgcgctgac cggtacgcag aataaggttc
     6181 agggaaattg gggcgagatg attctggaaa cggttttgga aaattccggc cttcagaaag
     6241 ggcgcgaata tgtggtgcag gcggcggctg tccgcaaaga agaagacggc ggcacgcgcc
     6301 gccttcagcc cgacgttttg gttaacctgc ccgacaacaa gcagattgtg attgattcca
     6361 aggtttcgct gacggcttat gtgcgctata cgcaagcggc ggatgcggat acggcggcgc
     6421 gcgaactggc ggcacacgtt gccagcatac gcgcgcatat gaaaggcttg tcgctgaagg
     6481 attacaccga tttggaaggt gtgaacacat tggatttcgt ctttatgttt atccctgtcg
     6541 aaccggccta cctgctggcg ttgcagaatg acgcgggatt gttccaagag tgtttcgaca
     6601 aacggattat gctggtcggc ccgagtacct tgttggcaac cttgcggacg gtggcgaata
     6661 tttggcgcaa cgaacagcaa aatcaaaacg cgctggcgat tgcggacgaa ggcggcaagc
     6721 tgtatgacaa gtttgtcggt ttcgtacaga cgctcgaaag cgtcggcaaa ggcatcgatc
     6781 aggcgcaaaa cagctttcag acggcattca agcaacttgc cgaagggcgc gggaatctgg
     6841 tcggccgcgc cgagaaactg cgtctgttgg gtgtgaaggc aaacaagcag cttgcacgcg
     6901 atttgaccga acgtgccaat gaaacaacgg cgttgtcgga atctttggaa tatgcggcag
     6961 aagatgaagc agtctgactt gtgcggaaaa atattgtttc agcaggggcg ggaatgccga
     7021 aagcgcggcg taactgtacc ggttctgatt tggggtttgt ttgaagtact taccaaagcc
     7081 ttgtccggcg aggtacaccg gcaaggcggc ggttgtctga gccttttgcg tttcagacgg
     7141 catcagtgca gatgacgccg tctgaagacc gtagtgaagt cggttagaaa aacggattgt
     7201 gccgtttttc gtgtccgatg gaagtcatac gcccgtgtcc ggcgacaact tgcacggttt
     7261 cggggagggc gaataatttg ttgcggatat tattgattaa gtcggcgtgg ttgccgcgcg
     7321 gaaaatcggt tctgcctatg gtttcgtaaa acagcacgtc gcccgcaatc agcagctccg
     7381 cttcggcaca atagaaaacc acgtgtcccg gcgtgtgccc cggaatatgc agcacttgga
     7441 aggcgtagcg tccgactttg agcgtttcgc cttcttcgag ccaatggttc ggcgcaaagg
     7501 cgggcgagac ggggaatccg tattgcgcgg tggtttgcgg cagcgattgg agcaggaatt
     7561 catcgtccgg atgcggcccg aggacaggga ctttatgcgt tttcaacatt tcgaccacgc
     7621 cgcccgcgtg atcgagatgg ccgtgcgtca gccagattgc cgtgagcgta agtttgcggt
     7681 ttgccaacgc ttgcagcagg aacggcacgt cgccgccgac atcggtcagg acggcttcgc
     7741 cgctttcgtc gtcccaaatc agggtgcagt tttggcggaa gggggtaacg gagaggattt
     7801 cgtaacgtag ggtcatggcg tgttcctaaa cggtttttca gacggcatcg ggtttgccgt
     7861 ttgttttatg gcggtttgcc gcctgtttgg atattggggg aatcagatga ttaagaagac
     7921 aatcggcggc atcataccga tttttacggc ggttttcatc cctgcatcag caggcgcggc
     7981 ggatttgatg ctggcgcagg aatacaaaga ccaagagatt gcaggctggg caatgagcga
     8041 gaaactcgac ggcgtgcgcg cctattggga cggaaagcac ctgataagcc gtcagggcta
     8101 cgcgtttacg ccccccaaag gctttaccgc gcagtttccg ccttatcctt tggacggcga
     8161 attgtatagc ggacgcggtc agttcgagca gatttccgct accgtgcgtt ctgtttcttc
     8221 agactggcgc ggcatccgcc tgcacgtttt cgatgtaccc aaggcgcagg gcaatctcta
     8281 ccaacgtttg gcagtcgcaa cgcagtggct gaaaacgcat ccgaacgcgc cgattaccat
     8341 catcccgcaa accaaagtgc gcgaccggca gcacgcgatg gactttttaa aacaaatcga
     8401 agcgcagggc ggcgaaggcg tgatgctgcg tcagcccgaa tcccgttata gcggcggcag
     8461 gagcagccaa ttattgaagc tgaaaagcca atacgacgac gaatgcacgg taacacggca
     8521 ctatgagggc aaagggcgaa acgccggacg gctgggcgcg gtcggctgca aaaaccgaca
     8581 cggcgaattc cgcatcggca gcggtttcaa agacaaagac cgcgacaacc cgcccaaaat
     8641 cggcacactg attacctacc gctaccgtgg ttttacgcgg aaaggcacgc cgaaatttgc
     8701 cacatttgtg cgcgtgcgta ccgaccgctg agcaggacgg ttcagacggc agccgatact
     8761 ttggtttata attttccttt taccgaccga ttccgacata tgaccgattt agaaaccaaa
     8821 cgccttgaaa cacaggcgat gcttgaaaac gccgatcttt tgttcgacca aggccaatgc
     8881 cgtgccgcac tgcaaaaagt ggcggacgag attacgcgcg atttgggcgg caaatatccg
     8941 ctgctgctgc ccgtgatggg cggcgcggtg gtgtttacgg ggcagttgct gccgctgttg
     9001 cgttttccct tagattttga ttatgttcac gtttcccgtt acggcgacaa gctggagggc
     9061 ggcgcgttca actggaagcg tatgcccgat gcggaacaaa tccggggcag gcacgtcgtc
     9121 gtgctggacg atattttgga cgaagggcat acgatgtccg ccattcaagc caaacttttg
     9181 gaaatgggtg cggcaagctg ccgtgcggcg gtgttcgcca acaagctgat cgacaaaccc
     9241 aagcctatcc gagccgatta tgtcggactg gatgtgccga accgttatgt tttcggttac
     9301 ggcatggatg cggcgggctg ctggcgcaat ctgggcgaga tttacgcatt ggacggaaaa
     9361 taaggaagcg atgccgtctg aaggcctttc agacggcatc gcgcccatac gccggtagga
     9421 taatgaagaa caggacgcat caatatgata gggcttttaa tcatcacaca cgaaaccata
     9481 ggcgaagcct accgcaagct ggcacatcat tttttcccgg gcgggctgcc tgaaaacgtc
     9541 cgcatactcg gcgtgcagcc gacggaagac caagacgaca tcatcaacaa cgccattgcc
     9601 gcgcttcagg agtttcccga caacgacggc gtgctgatta tgaccgatat tttcggcgcg
     9661 accccctgca atgccgcccg ccgcctcgtg cgcgaaaaca aatcggcgat tttgaccggg
     9721 ctgaacgcgc cgatgatggt taaggccgtc caatattcgc cggcggcgga agaccttgcc
     9781 gcctttaccg aatgcgtcag ggaggcggcg gtaaaaggca ttttcgccat cacgtccgcg
     9841 cccgaagatt tggtgtgccg gcgcagcggc gatgccgtct gaagaagcgg cagggcagga
     9901 aaacatttta aacggcgtct gctgccgata tacataacac gggaatcgaa atgctcaaac
     9961 aatccatcga aatcatcaac aaactcggac tccacgcccg cgcgtccaac aagttcaccc
    10021 aaaccgcgtc ccaattcaaa agcgaagtct gggttacgaa aaacgacagc cgcgtcaacg
    10081 gcaaaagcat tatggggctg atgatgctcg ccgccgccaa gggtacggtc atcgaactgg
    10141 agacggacgg cgcggacgag gcggaagcga tgcgcgccct gaccgactta atcaacggct
    10201 acttcggcga gggcgaataa tgagtatcgt gctgcacggc gtggcggcgg gcaaaggcat
    10261 tgccgtcggt tgcgcccacc tgattgcgcg cggtacggag gaagtgccgc agtatgatgt
    10321 tgcggaggcg gacaccgatg ccgaagccga acgtttcgat gccgccgtca aagccacgcg
    10381 caaagagttg gaacagctcc gcagcgcaat tcccgaaaac gccccgaccg agttgggcgc
    10441 gttcatctcg ctgcacctga tgctcttgac cgatgttacc ttgtcgcgcg aacccgtcga
    10501 tattttaagg gaacaaaaaa tcaacgccga gtgggcattg aagcagcaga gcgacaaact
    10561 cgccgcccaa ttcgacaata tggacgatgc ctatttgcgc gaacgcaagc aggatatgct
    10621 gcaagtcgtc cgccgcatcc acaacaacct gatcgggcag ggcaacgagt tggaagttgc
    10681 cgacaacctg tttgacgaaa ccgttctgat tgcaaacgac ctttcgcccg ccgacacggt
    10741 tttgtttaaa gagcagcgca ttgccgcctt cgttaccgat gccggcggcc ccaccgggca
    10801 tacggcgatt ttgggcagga gcttggacat cccgtccgtc gtcgggctgc acaacgcgcg
    10861 caaactgatt accgaaggcg aaacggtcat tgtggacggt atcaacggcg tgttgattat
    10921 cgcgccggat gagtcggtgt tgaacgaata ccgccgccgt gcccgcgaat accgcagcca
    10981 caaacgcgat ttgaacaagc tcaaaaaaac cgccgccgcc accgccgacg gggtctgcat
    11041 cgagcttgtg ggcaatatag aatccgccga agacgtgaaa ccgctgcaca acctcggcgc
    11101 agacggcatc gggctgttcc gcagcgagtt tctttacctg aaccgcgata cgatgccgtc
    11161 tgaagacgag cagtacgaag tgtacagcgc gattgtcaaa aaaatgaaag gcaaaagcgt
    11221 aacgatacgg acagtcgatt tgggtgtgga caaaaacccg cgctggttcg ggaaaaacag
    11281 cacgcccaac ggcagcctca accccgcgct gggcatgacc ggcatccgcc tgtgccttgc
    11341 cgaaccggtc atgttccgca cccagatgcg cgccatcctc cgtgcggcgg tacacggccc
    11401 cgtgcggatg atgtggccga tgattacctc cgtatccgaa gtgcgccagt gcctcatcca
    11461 cctcgacacc gcgcaacgcc agcttgccga acgcggcgat gccttcggta aagtcggcat
    11521 cggctgtatg attgaaattc cgtctgccgc gctgaccgtc ggcagtattt tgaaactggt
    11581 cgatttcatc tccgtcggta ccaacgacct gattcaatac atcttgtccg tcgatcgcgg
    11641 cgacgacagc gtcagccacc tctaccagcc cggccatccc gccgtgctga aaatgctgca
    11701 acacgtcatc cgtaccgcca accgcatgga caaagacgta tccgtatgcg gcgagatggc
    11761 gggcgatacc gcgtttaccc gcgttttatt gggtatgggg ctgcgccgtt tttccatgaa
    11821 ccccaacaac atcctgcccg tcaaaaacat cattctgcac agcaatgtcg gacagctcga
    11881 aagcgatatt gcgaaagtca tccgctgcga agacgaagag aagtcggaaa agctgatcaa
    11941 acagatgaac agcgtgtctg tcgaggaaga agccgacttc aaggggcgga aataaatacg
    12001 gcaggtaaaa aatagaaata cttaacaatg cccgcaatct gaaattttgc cattcttgca
    12061 aaatagaaaa ccgaaacaga aacccaaaat cggtcattcc ctcaaaatca gaaacctaaa
    12121 atccgtcatt cccgcgcagg cgggaatcta ggtttgtcgg cacagaaact tgtcgggaaa
    12181 aacggtttct ttagatttta
//
LOCUS       DAXHMX010000054        12176 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHMX010000054 DAXHMX010000000
VERSION     DAXHMX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.054"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            244..981
                     /locus_tag="ACFM4S_001059"
     CDS             244..981
                     /locus_tag="ACFM4S_001059"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6050757.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGCELIRED"
     gene            984..1670
                     /locus_tag="ACFM4S_001060"
     CDS             984..1670
                     /locus_tag="ACFM4S_001060"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6050758.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1829..2692
                     /locus_tag="ACFM4S_001061"
     CDS             1829..2692
                     /locus_tag="ACFM4S_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6050759.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2754..3323)
                     /locus_tag="ACFM4S_001062"
     CDS             complement(2754..3323)
                     /locus_tag="ACFM4S_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6050760.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3481..4341
                     /locus_tag="ACFM4S_001063"
     CDS             3481..4341
                     /locus_tag="ACFM4S_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6050761.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4477..4815
                     /locus_tag="ACFM4S_001064"
     CDS             4477..4815
                     /locus_tag="ACFM4S_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050762.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5183..5536
                     /locus_tag="ACFM4S_001065"
     CDS             5183..5536
                     /locus_tag="ACFM4S_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6050763.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5526..6392
                     /gene="atpB"
                     /locus_tag="ACFM4S_001066"
     CDS             5526..6392
                     /gene="atpB"
                     /locus_tag="ACFM4S_001066"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6050764.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6449..6685
                     /gene="atpE"
                     /locus_tag="ACFM4S_001067"
     CDS             6449..6685
                     /gene="atpE"
                     /locus_tag="ACFM4S_001067"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6050765.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6756..7226
                     /locus_tag="ACFM4S_001068"
     CDS             6756..7226
                     /locus_tag="ACFM4S_001068"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6050766.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7231..7764
                     /locus_tag="ACFM4S_001069"
     CDS             7231..7764
                     /locus_tag="ACFM4S_001069"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6050767.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAIIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7775..9322
                     /gene="atpA"
                     /locus_tag="ACFM4S_001070"
     CDS             7775..9322
                     /gene="atpA"
                     /locus_tag="ACFM4S_001070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6050768.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9347..10222
                     /gene="atpG"
                     /locus_tag="ACFM4S_001071"
     CDS             9347..10222
                     /gene="atpG"
                     /locus_tag="ACFM4S_001071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6050769.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10260..11657
                     /gene="atpD"
                     /locus_tag="ACFM4S_001072"
     CDS             10260..11657
                     /gene="atpD"
                     /locus_tag="ACFM4S_001072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6050770.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRAIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11668..12090
                     /locus_tag="ACFM4S_001073"
     CDS             11668..12090
                     /locus_tag="ACFM4S_001073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6050771.1"
                     /translation="MSIMEVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         2990 a         3119 c         3141 g         2926 t
ORIGIN      
        1 ttcaattatt tgccgttttg gtgcgaaccg ctgcctttgc ccgtttcaga cggcattgtc
       61 cgaaatggtt gcctgcttcc tgccttattg acaaaaaaat gctttcccga taatatccta
      121 cgaaaattaa cctgccgatt tgacacagct tgcgggcata acagctaaag cgttccgaca
      181 attccagctt tatcttccgc gcccgttgtg tccgacatcg ggctttgttg tatgggaaag
      241 acaatgatta ttttggacaa ggtttccaag cattaccaaa cgcgcgacaa gacccgtttt
      301 gccgccgtcg agccgaccag cctcgaaatc cgcgacggcg aaatcttcgg gctgatgggc
      361 tattccggcg cgggcaaatc caccctgttg cggctgatta acctgttgga acgccccgac
      421 acgggcaagg tcaacgtctg cggacaagag ctgaccgcgc tcgatgccgc cgcattgcgt
      481 caggctcggc agaatatcgg catggtgttt cagcagttca acctgttgaa caaccgcacc
      541 gtcgccggca acgtcgcctt cccattggaa atcgccggat ggccgtctga aaaaatcaaa
      601 gcgcgcgttg ccgaatgtct cgaaatcgtc ggactgaccg aacgcgccgg ccactatccc
      661 gcccagcttt caggcgggca gaaacagcgt gtcggcatcg cccgcgcact cgcgcccaaa
      721 ccccaagtca tcctcgccga cgaacccact tccgccctcg accccgccac cacgcgcagc
      781 gtcttggaat gtttggaaga catcaacaaa cgattcaacg taaccatcgt catcgtaacc
      841 cacgaaatga gcgttatcag acgactgtgc gaccgcgccg ccctgttgga taaaggcaaa
      901 gtcgtcgaaa tcgtcgaagt acgcggcaac caaatccacg cccgatccga catcgggtgc
      961 gaactgattc gggaggactg aatatggcag gcttaacatt tgaacaaacc gtttccaaca
     1021 tcatcggtat gaaaggcgaa atcgtccgcg cattgggcga aaccttcgta atggtcggtt
     1081 tgtccaccac attcgccgtc gttttcggca cgttgctggg cgtgttgctc ttcgtaactt
     1141 ccagccgcca actgcattac aacaagccgg tgaacttcct gctcgacaac ctcgtcaacc
     1201 tcatgcgcgc cttccccttc gtcattttga tgattgcgat gatacccgcc acacgcgcca
     1261 tcgtcggcag caccatcggc ccggttgccg cctcgctggt gttgagcgtg tcgggattgt
     1321 tttattttgc ccgactggtg gaacaaaacc tgcgcgaagt ccccaaaggc gtaattgaag
     1381 ccgccgccgc gatgggtgcg ccgccggttg ccatcgtccg caaagtcctc ttaaacgaag
     1441 cgcgcgcggg catggtttcc agcattaccg tgcttgccat cgggcttttg tcatacagcg
     1501 cggcggcagg gatgataggc ggcggcggct tgggcgacct cgccatccgc tacggctact
     1561 accgctacca aaccgaagtc atcgtcttca tcgtcgccct cctcgtgctg ctggtcatcc
     1621 tgattcaaag caccggcaac gcattggcgc ggaaattgga taaacgttaa acccgaatgc
     1681 cgtctgaacg ccaaaacccc caccgctatc cgaaaaatgc tataaaatcc ccctgttcgc
     1741 ggcaaatgcc gtctgaacgc cgaatctgga cggcaggact ccctgcccgt catttttgtt
     1801 tgaaactgcc acaacatcag gagaaaatat gaaaaccttc ttcaaaaccc tttccgccgc
     1861 cgcactcgcg ctcatcctcg ccgcctgcgg cggtcaaaaa gacagcgcgc ccgccgcatc
     1921 cgcttctgcc gccgccgaca acggcgcgga gaaaaaagaa atcgtcttcg gcacgaccgt
     1981 cggcgacttc ggcgatatgg tcaaagaaca catccaaccc gagctggaga aaaaaggcta
     2041 caccgtcaaa ctggtcgagt ttaccgacta tgtgcgcccg aatctggcat tggctgaggg
     2101 cgagttggac atcaacgtct tccaacacaa accctatctt gacgacttca aaaaagaaca
     2161 caatctggac atcaccgaag tcttccaagt gccgaccgcg cctttgggac tgtacccggg
     2221 caagctgaaa tcgctggaag aagtcaaaga cggcagcacc gtatccgcgc ccaacgaccc
     2281 gtccaacttc gcccgcgtct tggtgatgct cgacgaactg ggttggatca aactcaaaga
     2341 cggcatcaat ccgctgaccg catccaaagc ggacattgcc gaaaacctga aaaacatcaa
     2401 aatcgtcgag cttgaagccg cgcaactgcc gcgtagccgc gccgacgtgg attttgccgt
     2461 cgtcaacggc aactacgcca taagcagcgg catgaagctg accgaagccc tgttccaaga
     2521 accgagcttt gcctatgtca actggtctgc cgtcaaaacc gccgacaaag acagccaatg
     2581 gcttaaagac gtaaccgagg cctataactc cgacgcgttc aaagcctacg cgcacaaacg
     2641 cttcgagggc tacaaatccc ctgccgcatg gaatgaaggc gcagccaaat aaggcagtcg
     2701 tataaaatga tgccgtctga accgtatccg tgttcagacg gcatttttgt cctttaatcc
     2761 gccattccct gccattccgc cgaatccggc gtatcgattc cgaacagcga caaagcgtgt
     2821 gcaacactgt gcgccactat gtcgtccgcc gtctgcggtt tgcggtacat cgcaggaaca
     2881 gggggaaaca ccacgccgcc catttccgtt acccgcttca tattgtccaa atgggcaagg
     2941 ttcagcggcg tttcgcgcac catcagcacc agccgccgcc tttccttcaa aaccacatcc
     3001 gccgcacgcg tcaagaggtt gtcgccgaag ccgtgcgcga ccgaggcaag cgtccgcatc
     3061 gaacaggggg cgaccagcat cccgtccgtt ttaaacgtac cgctggcaat gcacgccccg
     3121 atattgccga tcggatgcac gaagtccgcc aaggcatata cctcatctct cgcataagcc
     3181 gtttccgaag cgcgcgccat ctccgcacct ttcgatacca caaggtgcgt ttcgatatct
     3241 tgcgcgcgta aaagttccaa agccttcacg ccgtattgga aaccgctcgc cccactgatg
     3301 ccgattatca aacgccgtac catcatccgc ctttcccata aaaccgcctg caacggcaaa
     3361 ccggctatta tagtgaaaaa acagaaatcc gataaatacg gatacaaatt gtcggcaaca
     3421 cccaatatcc gataaaatat ccgatttaac atcctatctg aataggcacg ggagggcggt
     3481 atggcaaaag taaaaggcgg attggggcgc ggtttggatt cgctgctcgc caacggcgca
     3541 gacaacagca gcggcgaccg attgaccacg gttgcggtta aagatatccg gcccggccgc
     3601 tatcaggcgc gtgttcaaat cgatgacgaa gccttgcagg agctggcgga ttcgattaag
     3661 gcgcaaggcg tgatacagcc cgtcatcgtg cgcgaacacg gactgtcccg atacgaactg
     3721 attgcgggcg aacgccgttg gcgcgccgca cagattgccg gcctgaccga aatccccgcc
     3781 gttatcaaaa ccatcagcga cgaaaccgca ttggcaatgg gtttgatcga aaacctccag
     3841 cgcgaaaacc tcaaccccat cgaagaagca caaggcttga aacgccttgc cgacgagttc
     3901 ggactgaccc acgaaaccat cgcccaggcc gtcggtaaaa gccgaagcgc gatttccaac
     3961 agcctgcgcc ttttaagcct gcccgaaccc gtgcaggaaa tgctttacca acgccgcctc
     4021 gaaatgggac acgcccgcgc attgctgacc ctgcccgtcg tcgaacagct cgagttggcg
     4081 caaaaggccg tcaaaaacgg ctggtcggtg cgcgaagtcg agcgccgcag ccaagccgcc
     4141 cttcaaaaca aacgtcccga gcccaaaaag actgccgccg ccgacatcgg ccgcctgaat
     4201 gatttgctga ctgaaaaact gggtgtcaac gccgaaatca aaaccgccaa ccataaaaaa
     4261 ggcagaatca tcctgcattt cgacacgccc gaaaccttcg accatatctt gaagcagttg
     4321 ggcatagatt accgacctta attttgcggg atataccgtc tgaaatatag agaatagctt
     4381 tccagatttt aagtgggaaa tataattcta ttgacatttt ttctgcttca cgtaagaatc
     4441 gttttcctgt tttcattttt aattttcgag gaaattatga acacacgcat catcgtttcg
     4501 gctgcgttcg ttgcgttggc attagcaggt tgcggctcaa tcaataatgt aaccgtttcc
     4561 gaccagaaac ttcaggaacg tgccgcgttt gccttgggcg tcagcccaaa tgccgtaaaa
     4621 atcagcaacc gcagcaatga aggcatacgc atcaacttta ccgcaactgt gggtaagcgc
     4681 gtgagccaat gctatgttac cagtgtaatc agcacaatcg gcgttaccac ttccgatgca
     4741 atttgtttgg gaggcggaac gcacaaaggc aaaagtcaat gcaatgcttt gcttaaagcg
     4801 gcaggccgtt gctaatcctt tattcggaaa aggtcgtctg aaaatatttt cagacgatct
     4861 tttatttatt gagcaaattc cccaaactgc cgacaatccg accgataaac tgctacaatt
     4921 tcgcgttgat tttttattat ttccacgtaa agcaaacaaa tcacgtaaga acattaaaag
     4981 tgtaaagaaa ttgcggacaa actccttaat atcctgtaat tatatgtaat gtgtgttaga
     5041 atgacacctg tcgccgttat tcaggcggcg agcgcggcgg attgggatac gttgtttcac
     5101 aaagattaac tgtcgcttga cgtaataagg cttctaaatt atattagccg cgccccaaat
     5161 ccgaggcgga aacattccct ttatgaagca gattatcatc ctacaatccg ccgtattgtc
     5221 catatgcgcc gcagttgcct ttgctgtatg gggttttgcc ggattccttt cggcggtcgg
     5281 tggcggtttg tcctacctgc ttcccacgtt tgttgcagtt ttacttttaa aacttttcag
     5341 gggaaaccct ttcctgcaaa gcaggatgtt cgtcttcgga gagattttaa aagtagtgct
     5401 gtcgctgttg tccatgctcg ccgtatttgc tgtatggcat caatcgttgg tgttcgcccc
     5461 gtttctgatg gggctgctcg gtgtcagcca tttggttttt ttagtattgt tgagagtgaa
     5521 agattatggc aggtgaaacc attaccgctg ccgactacat caagcaccac ttgcagagct
     5581 tgaccagttt gtcggatgtt actcagggtc agggactgaa aaacattgct gatttttcgt
     5641 ttattaacct tgatgccgtc ttttttgccg tcctgttggg cgtaatcggc agcttcctgt
     5701 tgtggcgcgg tgcgaaaaaa gcaacggcag gcgttcccgg acgtttccag gctgccgtgg
     5761 aaatcttgtt tgagtttgtg gacgatatgt gtaagagcat cattcacaac gaaaagtcta
     5821 gaaaagccgt cgcgccgttg ggtctgacgc tgtttgtctg gatttttctg atgaacgcga
     5881 tggatatgct gccggtcgat ttgctgccga tggtatggca gggcattacc ggcaaccatc
     5941 acgccctgct gcgcgtcgta ccgactgccg atttgaacac cactttggca ctcgctgtcg
     6001 gcgtgttgct gatttgtatc tattacaaca tcaaaatcaa aggattgggt ggctggtttc
     6061 acgagttgtt cagcgcgccg ttcggtgcaa aactcgcacc tgcgaacttt ctgttgaatt
     6121 tggtcgagtt tctttctaaa accgtatccc acggtatgcg gttgttcggc aatatgtatg
     6181 caggcgagtt ggtattcttg ttgatagcct tgcttggcgg tgcttgggcg gcttccggca
     6241 gcgtcgaagt catggatccg attctgtttg tattccacat tattgccggt ttggcatggg
     6301 cgattttcca tattttggtg attaccctgc aggcgtttat tttcatggcg ttggcgttcg
     6361 tctatatcgg acaggcacat gatgcgcact gattttccta agtagtttgt ttttgtagtt
     6421 taacctttgt ttctttaagg agtttaaaat gggtttgatt gctatcgcat gtggtttgat
     6481 cgttgcattg ggtgcattgg gtgcctctat cggtatcgca atggtcggtt ctaaatattt
     6541 ggagtcttct gcccgtcagc ccgaactgat cggtccgctg caaaccaaac tgttcctgat
     6601 tgccggtttg attgatgccg cattcttgat tggtgtcgcc attgcactgc tgttcgcctt
     6661 cgttaacccg tttgcaggtg cataatcagg acggattcgt ccgttccgta caggcgcagc
     6721 ctgtttgatt aaccccagta cgaaggttaa gtaacgtgaa tatcaatgca acattattcg
     6781 ctcaaatcat cgtctttttc ggtttggtat ggtttaccat gaaattcgtg tggccgccga
     6841 ttgcaaaagc tttggatgag cgtgccgcaa aagtcgccga aggcttggct gccgccgagc
     6901 gtggtaaaag cgattttgag caggctgaaa aaaaggttgc agaactcttg gcagaagggc
     6961 gtaatcaggt ttccgaaatg gttgccaacg ccgaaaaacg tgccgccaaa attgtcgaag
     7021 aagccaaaga acaggcttct tccgaggcgg cgcgcattgc agctcaggca aaggccgatg
     7081 tggagcagga attgttccgc gcacgcgaat ccctgcgcga gcaggttgcc gtgttggctg
     7141 tcaaaggtgc cgaatctatt ttgcgcagcg aagtcgatgc ttccaaacac gcaaaactgc
     7201 tcgataccct gaaacaggag ctgtaatctt atggcagagt tcgcaacgat tgccagacct
     7261 tatgcaaagg cattgttcgg tctggctcag gaaaaaaacc aaattgagtc ttggttgggc
     7321 ggactggaaa aacttgcggc ggttgttcag gaagggaagg tggcttcatt gattgaccgt
     7381 cctgaaacga atgcttcaga aaaagcagat atcctcatcg atttggtcgg tttgaaagac
     7441 aaggagttga aaaactttgt tatcgtcttg gccgggcaga aacgtttgtc gatattgccg
     7501 gaagtatatg ctcaatatca agacttgacc ttatcattca accatatcaa atctgccatc
     7561 atttacagtg cctatccgtt gaccgacaaa caggtcggcg agttggcgca aatgctgaat
     7621 aagcgtttcg acagcgagct gaaaatctct gtcgaaatcg aaccggagct gattggcggc
     7681 ataaaagttg aagtgggtga tcaggttttg gatttgtctg tacaaggcaa attgagtgcc
     7741 ttgtacacga ctatgacgaa ttaggagagt tttcatgcag cttaatcctg ctgaaattag
     7801 cgatttgatt aaagccaaga tcgaaaatct gtctgtaaat gccgaagtgc gtacccgtgg
     7861 tacggtgatt tcggtaactg acggtatcgt tcgcatccat ggtttgtcag atgcaatgca
     7921 aggtgagatg ctcgaattcc cgggtaacac tttcggcttg gcgatgaacc tggagcgcga
     7981 ctccgtcggc gccgtagtgt tgggcgagta cgaacatatt aaagaaggcg acacggttac
     8041 ctgtaccggt cgtatcttgg aagtgccggt cggacgcgaa ctggtcggac gcgtcgttga
     8101 tgcattgggt cggcctatcg acggcaaagg cccgattaat acaactttga ccgcccctat
     8161 cgaaaaaatc gcaccgggcg tgattgcgcg caaatcggtt gaccagccga tgcaaaccgg
     8221 tctgaaggcg attgactcta tggttcctgt cggtcgcggt cagcgtgagt tgatcattgg
     8281 tgaccgtcaa accggtaaaa ctgccgtagc actggatgcc atcgtcaacc aaaaaggtac
     8341 gggtgttatc tgtatctatg tcgctatcgg tcaaaaagca tcttctattg ccaacgtggt
     8401 ccgcaaattg gaagagcatg gcgcgatgga acacacgatt gtggttgctg caactgcttc
     8461 tgaagccgct gcactgcaat acatcgcacc ttactccggt tgtacgatgg gtgaattctt
     8521 ccgcgaccgc ggcgaagatg ccttgattgt ttatgacgat ttgtccaaac aggctgtggc
     8581 ttaccgtcaa atttccctgc ttttgcgccg tccgcccggc cgcgaagcct atcccggcga
     8641 tgtgttctac ctgcactccc gtctgttgga acgtgcggca cgtgtcaatg aacacgaagt
     8701 ggagaaattg accaacggcg aagtaaaagg caaaaccggt tctctgactg ctttgccgat
     8761 tatcgaaacc caagcgggcg acgtatctgc tttcgttccg actaacgtca tttcgattac
     8821 cgacggtcag attttcttgg aaaccgacct cttcaacgcc ggtatccgtc ctgcaatcaa
     8881 tgccggtatt tccgtatccc gcgtaggcgg tgctgcacaa accaaagtga ttaaaaagct
     8941 gggtggcggt atccgtttgg cgttggcgca atatcgtgaa ttggcggcgt tctcgcaatt
     9001 tgcatccgat ttggatgaag ctacgcgcaa acaattggag catggcgaag tcgtaaccga
     9061 actgatgaaa cagaaacagt tcagtacgtt gaatacggct gaaatggctt tgaccctttg
     9121 ggcaatcaac aacggttcgt attctgatgt tccggttgcc aaagctctgg cttttgaatc
     9181 tgaatttttg agctttgtcc gtacccaaca tccggaagtt ttggaagccg tcaatgcttc
     9241 aggtgcaatg tccgacgaga gcgagaaaac gcttgaagca gccatgaaat ccttcaaatc
     9301 ttcttacgcc tatcaggcat aaggcttgaa gtgaaaggag tctgaaatgg cagtaggaaa
     9361 agagattctc accaaaatcc gcagtgttca gaatacccaa aagatcacta aagcgatgca
     9421 gatggtgtca acctctaaaa tgcggaagac tcaggaacgg atgcgtttgg cgcgtccgta
     9481 tgccgaaaaa gtgcgtatgg tgatgagcca tcttgcgcaa accaataccg atcatggtat
     9541 tccgttactg gaatctcatc gggaaatcag acgtgtcggt tttattttga ttacgtctga
     9601 taagggtttg tgtggcggtt tgaacgccaa cgtgctgaaa aagtttttgg cacaagttca
     9661 agagtatcgg aatcaaggta ttgaagagga aatcgtatgc cttggcagta aaggtctgat
     9721 ggcgtgtcag agcattggtc tgaatgtggt tgccagtgcc gtaaatttgg gcgatacccc
     9781 aaaaatggaa atgctgctcg gacctttgac agaactcttc caacggtatg agaaacatga
     9841 aattgacaga atccatctgg tgtattcggg ttttgtcaat accatgcgtc aagaaccgag
     9901 aatggaagta ttgctgccta tcggtgagaa cgtgattggc gattcagctc ccaaatcacc
     9961 gttcagctgg gaataccgct acgaaccgac tgcacttgca gtgttggaat atctggttcg
    10021 ccgctattta gagtctgtgg tttatcaggc gttgagcgac aatatggcat ccgaacaggc
    10081 agcccgcatg gttgccatga aagctgcaac agacaatgca ggcaatgcca tcaaagagtt
    10141 gcgtttggta tataacaaat cgcgtcaagc tgcgattacc acggaattgt cagaaattgt
    10201 agcaggtgcg gcggctgtct gatgccgtct gaatcctgta cggaaattag gatacgataa
    10261 tgagccaagg caaaatcgta caaattatcg gtgcggttgt tgacgtggaa tttccacgcg
    10321 acatgattcc gcgcgtttac gacgctttga aattagacga aaacggtctg actttggaag
    10381 tccaacagct tttgggcgac ggcgtagtcc gtgccattgc gatgggcagc tcggacggtt
    10441 tgaaacgcgg catgactgtg agcaataccg gtgcgcccat tactgtgccg gtaggtaaag
    10501 gtacgttggg acgcattgtc gatgtattgg gaacgcctgt tgacgaagca ggtccgatcg
    10561 ataccgacaa gagccgtgcc atccaccaag ctgcccctaa gtttgacgaa ctgtcttcca
    10621 caaccgaatt gctcgaaacg ggcattaaag tgattgactt gctgtgtccg tttgccaaag
    10681 gcggtaaagt aggtctgttc ggcggtgccg gtgtgggtaa aaccgtgaac atgatggaat
    10741 tgatcaacaa catcgccaaa gcgcacagcg gcttgtccgt gttcgcaggc gtgggtgagc
    10801 gtacccgcga aggtaacgac ttctaccacg agatgaaaga ttccaacgta ttggataaag
    10861 tggcaatggt ttacggtcag atgaacgaac ctccgggcaa ccgtttgcgc gtcgcattga
    10921 ccggtttgac catggcggaa tacttccgtg acgaaaaaga cgaaaacggc aaaggccgcg
    10981 acgtattgtt cttcgtggac aacatctacc gttacaccct ggctggtacc gaagtatccg
    11041 cattgttggg tcgtatgccg tctgcagtgg gctaccaacc gacattggca gaagaaatgg
    11101 gtcgtttgca ggagcgtatt acctctaccc aaaccggttc tattacttcc atccaagccg
    11161 tatatgtacc tgcggacgac ttgaccgacc cgtctccggc aacaacgttc gcccacttgg
    11221 acgcaaccgt cgtattgagc cgcgatattg cctctttggg tatttacccg gcagtcgatc
    11281 cgcttgattc tacttcgcgc caactggatc cgatggtatt gggtcaagag cactacgacg
    11341 tagcgcgcgg cgtacagtcc accctgcaga aatacaaaga attgcgcgac atcatcgcca
    11401 tcttgggtat ggacgaattg tctgacgaag acaaactgac cgtaatgcgt gcgcgtaaaa
    11461 tccaacgctt cctgtcccaa ccgttccacg ttgccgaagt gtttacaggt tctccgggca
    11521 aatatgtcgc cctgcgcgat accattgccg gcttcaaagc catcttgaac ggcgaatacg
    11581 accatctgcc cgagcaggca ttctatatgg tcggcagcat cgaagaagcg gttgagaaag
    11641 cgaaaacctt aaactgagga ggtcggcatg agcatcatgg aagttgaggt ggtaagtagc
    11701 gagcagaaaa tctattcagg cgaggcaaca tttatcgttg ttccgactgt acagggcgaa
    11761 ctcggtattt atccgcgaca cgagccgatt atgagtttgg tgcggccggg ggctttgcgt
    11821 ttgaccgttc cgggcgagga taaagaggtt ttggttgctg tttccggcgg tgttttggaa
    11881 gtacagcctg ataaagtaac tgtcttggcg gatgttgccg tccgcagtgc ggagatggat
    11941 caggcacgtg cggaagaggc gaaaaaagcc gcagaagcgg gcatttccca agctaaagac
    12001 gataaggctt tggcggaggc gcataaagca ttggctgctg cgattgcaca gctcaaaact
    12061 ttggactata tccgttcgca caagaaataa acttgtttgg tttggaaaag cacgaccgtt
    12121 tggtcgtgct ttttattttt ccaagttttg aaaatatagt ggattaacaa aaatca
//
LOCUS       DAXHMX010000055        12076 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHMX010000055 DAXHMX010000000
VERSION     DAXHMX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.055"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="ACFM4S_001074"
     CDS             complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="ACFM4S_001074"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6050772.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVIGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="ACFM4S_001075"
     CDS             complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="ACFM4S_001075"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6050773.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="ACFM4S_001076"
     CDS             complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="ACFM4S_001076"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6050774.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGNDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(2757..3257)
                     /locus_tag="ACFM4S_001077"
     CDS             complement(2757..3257)
                     /locus_tag="ACFM4S_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6050775.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="ACFM4S_001078"
     CDS             complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="ACFM4S_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6050776.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMFGGEVGIAGGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(5773..7113)
                     /gene="rseP"
                     /locus_tag="ACFM4S_001079"
     CDS             complement(5773..7113)
                     /gene="rseP"
                     /locus_tag="ACFM4S_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6050777.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPITSWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(7148..8383)
                     /gene="ispC"
                     /locus_tag="ACFM4S_001080"
     CDS             complement(7148..8383)
                     /gene="ispC"
                     /locus_tag="ACFM4S_001080"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6050778.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(8388..9185)
                     /locus_tag="ACFM4S_001081"
     CDS             complement(8388..9185)
                     /locus_tag="ACFM4S_001081"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6050779.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVIGLVWVADVCAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(9188..9934)
                     /locus_tag="ACFM4S_001082"
     CDS             complement(9188..9934)
                     /locus_tag="ACFM4S_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6050780.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(9990..10547)
                     /gene="frr"
                     /locus_tag="ACFM4S_001083"
     CDS             complement(9990..10547)
                     /gene="frr"
                     /locus_tag="ACFM4S_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6050781.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDACTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(10728..11855)
                     /locus_tag="ACFM4S_001084"
     CDS             complement(10728..11855)
                     /locus_tag="ACFM4S_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6050782.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(11931..12006)
                     /locus_tag="ACFM4S_001085"
     tRNA            complement(11931..12006)
                     /locus_tag="ACFM4S_001085"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11971..11973),aa:Thr,seq:cgt)
BASE COUNT         2276 a         3083 c         3760 g         2957 t
ORIGIN      
        1 tttaaaacac cacttttgta gtgtccagta atttcagcac agaatattca ataagacaat
       61 atgttctttt gaaaaatact tttggttttt tcgccgaaag caggtcggtt caagctgcgg
      121 aaattgtttg caattcttta aaagcagcgg cggaggtcac aatgaaatgt ccgaatgggg
      181 atgtggtcgg cggcagaaat catcaatgct gccgactgcc atacttctga aatctacaaa
      241 atgatgcatc gatcaaacaa tataccgctt taaaaaaacc gatgccgtct gaaacgcttt
      301 cggggtttca gacggcatca aaagggtacg gtcagcggat gatgccgcgc gccgattgtg
      361 cgaaaaagtc tcggaatacg gcaagctcgg cttgggtttc ggcgcggcgg aggatgtctg
      421 ccttggcctc ttcaaacgga atgccgcgat ggtagagggt tttgtacacg tctttgacgg
      481 cggaaatctg ttctgcggta aaaccgttgc ggcgcatgcc ttcgctgttg agccccgccg
      541 gttcggcgcg gtagcccgat gccataaagt agggcggtac gtctttgtgt acgcctgcgg
      601 cgaaggcggt catggcgtag tcgccgatgt ggcagaattg gaaaaccagc gtgtagccgc
      661 ccaaaacgac gtagtcgccg atggtaacgt gtccggcaag cgaggcgttg ttggcgaaga
      721 tggtgtggtt gccgatgacg cagtcgtgcg cgaggtggca gtacgccata atccagttgt
      781 cgtcgccgat acgggtttcg ccgatgccgg ttaccgtgcc taaattaaag gtggtgaatt
      841 cgcggatggt gttgccgttg ccgataatca gcttggtcgg ctcgtcgcgg tattttttgt
      901 cctgcgggat ttcgccgagg ctggcaaatt ggaaaatgcg gttgttttcg ccgatgctgg
      961 tgtggccgtt gatgacggcg tgcggaccga tttcggtatt cgcgccgatt tggacgttgg
     1021 ggccgataac ggtgtacgcg ccgactttga cgccggagtc gagttcggct ttggggtcga
     1081 tgacggcggt cgggtggatg agggtcatgt ttttcctttc ctgtcgtgtt gccgcgaaga
     1141 tgcgcgacgg caacaggttg tctgaaaact ttcagacgac ctttttctga acactcaaac
     1201 tacgcgtttg gcacacatga tgatggcttc gacggcaact tgcccgtcca ctttggcgac
     1261 ggcgttgaat ttgccgatgc cgcgccgact ggtcagcagc tcgacttcaa agacgagttg
     1321 gtcgccgggg atgacttggc gtttgaaacg ggcttcgtct atgccggcga agaagaagaa
     1381 ttcgttttct ttgcgcccgc cttcgctcaa aatcgccaac gtgccgcacg cctgcgccat
     1441 cgcttcgatg atgagtacgc cgggcatcac gggcaggtcg ggaaaatggc cttggaactg
     1501 gggttcgttt atggtgacgt ttttaatcgc ggtcagggtt ttcatcggct cgaaggcggt
     1561 gatgcggtcg agctggagaa acggatagcg gtgggggatg agtttttgga tgtctttggc
     1621 ttcgatgggg agttgtacgt ccatgtctgt cgtattcctt gaataaagtc ggtttggtta
     1681 tttgctgtct tgaccggcat ctgaaagctg ctgctccagt gttttgagcc gtttgttcat
     1741 ttcgcttaag cggtggatgt aaacagcgtt gcgcgcccat tctttatggg tggacatcgg
     1801 gaagatgccg gcgaggtgtt tgccgctttc ggtaatgctg tgggtgacgg acgtgccgcc
     1861 gccgatggtg gttttgtcgg cgatttcgat gtgtccgacc gtaccgacgc cgccgccgat
     1921 gatgcagtag ctgcctatgg ttacgctgcc tgagatgccg gttttggcgg cgatgacggt
     1981 gtgcgaaccg attttgcagt tgtgtccgat ttggacttgg ttgtcgattt tggtgccgtt
     2041 gccgacggtg gtgtcgctca tcgcgccgcg gtcgatgttg gtgttcgagc cgatttctac
     2101 gtcgttgccc agcgttaccg cgccggtttg cgggatttta aaccatgaat cgccggcaaa
     2161 agcgagtccg aaaccgtccg cgccgatgac cgcgccgctg tggatttcga cgcgtctgcc
     2221 cagtgtgcag ccgtaataaa cgacggcgtt gggatgcagg acgacttcgt cgcccagttt
     2281 gcaatcgtgt tggacgacgg cgtttgccaa gatgcggcag ccttcgccga gcacggtgtt
     2341 tgcgccgatg tagacgttcg cgccgatttc gcagctggcg ggaacggtcg cgcccggttc
     2401 gacgacggcg gtcggatgga tgccgccgcg cgctttgacg acgggtgaaa acaggcgggc
     2461 gactttggcg aaatagagat aggggtcgtc ggcgacaatc aggttgcgcc cttcaaatcc
     2521 gtctgccgct ttggcggaaa cgatgaccgc gcccgcgctg ctgtcgtgga cttcggcttt
     2581 gtatttcgga ttggcaagga agctgatgtg ttccgcctgc gcgtctgcga gcgggcgcac
     2641 ggcggtaacg gaaatgtcct cgccgcgcca ttcgccgccg agccgcgcgg tgatttggga
     2701 cagggtgtag gtggccggaa tcatggtttt cctgttcggt atgccgtctg aaagggtcag
     2761 cgggcgttca tttctttaat gacgctgtcg gtaacgtcgt attgggtgtt gacgtaaatc
     2821 acgttctgca aaatgacatc gtaaccttcc tgtttggcga ttttgacgat gacgcggttg
     2881 gcgttttgct ggagggaggc aaactcttcg ttgcggcgga ggttgtagtc ttcttcaaac
     2941 tgcgcctgtt ttttgcggaa cgctgcgacc agcccgcgcc atttttcttc ggcttgcgcc
     3001 ttttttgcgt ttctgagttt gccttcggca agctgccttt ccaaatccag accttcgcgt
     3061 tgcagttttt gcaattcgtc ctgacgggcg gaaaattcgc tgtccagcgt tttttgaatc
     3121 ttgcgcgcct gcttggattc gaggtagatg cgctcggtgt tgataaagcc gattttttgg
     3181 aaggtgtcgg cgtgcgcgcc tgcggtgcag cacaaaccga tcagagccgc ggcaaacgcg
     3241 cgggtcaaac gggtcatggt aaaactcctt cgaatgttgc cgcgaaatgc cgtctgaagg
     3301 gcttcagacg gcatttgcgg gattagaacg tcgtgccgag ttggaattgg aagcgttgga
     3361 tttcgtcttc cggttttttc ttcagcgggt aggcgtagct gaatttcatc gggcctaaag
     3421 gcgagagcca ggtaaccgcg ccgccggcgg aatagcgcaa ttcgttggta aaggtggatt
     3481 tatgggtatt gccggcgccg taaatgtttt gaaccctgcc gccggtcgcg gaactgctgt
     3541 tgtcgtcgta ggttttgccg tcccacacgc tgcctgcgtc ggcaaacagg ctcaggcgga
     3601 cggtgcgcgc gtctttcgcg ccgggcatcg ggaagagcag ctcggcggag acgttggctt
     3661 ttttgttgcc gccgtagctg attttttcgc cgtattcgtc atacactttc ggaccgagcg
     3721 tgccgctttc gtatccgcgc accgaaccca ggccgccgcc gtagaagttt tcaaagaagg
     3781 ggatttcttt ggttttgccg tagccgcccg caatgccgac ttcgccgccg aacatcagcg
     3841 tgaaggtttt gcttaagggg aagaaccagg tttggttgtg ggtggcggag tagtattgca
     3901 gtttgctgcc gggcagggcg atttcagcgt tcacgcccgt caggtagccg cgcgtcggcc
     3961 ataacgcgct gtcggttttg ttgcgccccc agccgacggt acctttgtac agccagcctt
     4021 tgaagctgcc gtctgtgccg tcggttttgc cgtatttctt gataaagtcg gcatagtgtt
     4081 tgggcgcttt gttgtaggtg ttgacggtca ggtgttctgc caccaaaccg aaattcacgc
     4141 ggtcgtattc ggtaacaggc acgctcatgc ggatgcctgc gcctgccgtg gtggttttat
     4201 attgtttgat gctggtcgat gctttgcgcg ggtcgaaggc ttttccgtaa acatcgtagc
     4261 ctaggctgac cccgtctgcc gtgaagtacg ggtcggtaaa cgacagcgag ccgttgagcg
     4321 tggttttgct tcgtgaggcg cgcagggcgg ccgacttgcc cgtaccgaac aggttgtctt
     4381 gggaaacgcc tgcggacatg accaggccgg tatcctgtac ccagcccgcg ctcaagtcga
     4441 gcgagccggt ggaacgttcg gtcaggctca tgttcaaatc gactttgtcg ggtgtgccgg
     4501 caagcgggac ggcatcaaac tgtacgttgt cgaagtagcc caaaagctcg acgcgctctt
     4561 tggagcgttg cagcttggag gtgtcgtaag gcgcggattc catttggcgc aattcgcggc
     4621 gcacgacttc gtcgcgggtt ttgttgttgc cggtgatgtg gatttcgttg acgtagattt
     4681 tccggcccgg ttcgatgtgc aggacgaaat cgacggtttt ggtttcggcg tttggcagcg
     4741 gctgtacgct gatttcgctg tatgcgtagc ctgccgagcc catgcggttc tgaatctcac
     4801 ccaaaacggc ggtcatctgc tggcgttcgt accatttgcc gggcttcatg gtcagcagtt
     4861 tttccagttc ggctttgggg acttcgttgg tgtcgccttc gatggagact ttgccccaac
     4921 ggaaacgtcc gccttcgtgg acggtgattt tgatggtctg cttggttttg tcttcgttgg
     4981 tttggatgtc ggtatcgagg atgcggaaat cgaagtagcc gttgttctgg tagaagtcgg
     5041 ttactttttc catgtcttgg gcaaatttct gctcgttgaa ttggttgctt cgtgtcagcc
     5101 atgtccaaat gccgccttcg gtcagcgaca tctgccgcat cagtttgcgg tcggaataga
     5161 cttggttgcc ttcaaattcg atgtcggtga ttttggcgga tttgccctcg tcaatcgtga
     5221 tgtcgatgtc gacgcggttg cgggcgagtt tggttacttt gggcgtgatt tggatattga
     5281 gtttgccgcg cccgaggtat tcttctttca ggccggcgac tgcctgattg agtgtcgcct
     5341 gattaaagta ttgcgactgc gccagcccga acgattcgag gtttttctta atggcgtcgt
     5401 tttgcagcat ttttgcgccg gtgatgttga gcgagccgat ggtggggcgt tcgataacgg
     5461 tcagcaggag ctgcccgtcc gcagtttcga cgcgtacgtc gtcaaagaaa ccggtggcgt
     5521 acaggctttt gatgatggca ctgccgtgtg tgtcgttgta ggtgtcgccg actttgacgg
     5581 gcaggtagtt gaatacggta ctcggctcgg tacgctgcaa gccttcgacg cggatgtctt
     5641 ggatggtgaa gtcggcaagt gccaaaggcg atatgcccaa gaccatcagt gcggaagcaa
     5701 tctgtttcag tttcattgtc agttccttgt ggtgcggaat gcggtttcag acggcattcc
     5761 gaaacgtaaa atctaaccga gcagccgggt aacgtcgttg aagaaggcga ccgccatcat
     5821 cagcatcatg agggcaagcc cgaagcgcaa accgatgttt tggacgcgtt cgcccaaagg
     5881 tttgccgcgt atccattcgg cagtataaaa cacgaggtgg ccgccgtcca aaacggggac
     5941 gggcagtagg ttcagcacgc cgaggctgat gctgaccagt gccaaaaatt ccaaataact
     6001 ttgcaagccg agttcggcgg actgtccggc aatgtcggca atggtcagcg gcccggaaat
     6061 atggctgacg gaggcgttgc cgctgattag tttgccgaaa aatttgaggg ttgtccacga
     6121 gtgggaaacg gttttttccc agcccatgcc gaatgcgcgg acaacagacg gacggtagct
     6181 gcggcggatt tgcgcgtccc acgccctgtc cggctgcgga cggaggccga cgcgcccgat
     6241 cagggtgtgg tcgggctgtt cgacagtatc ggggcggatg tcggcggtat gggtttgtcc
     6301 ggcgcgttcg tagttcaggg tgattttttt gccggggctt tggcgggtca ggtttgccca
     6361 ttcttgccat gaggtaatgg gtttgccgtc ggcggcagtc agcctgtcgc ccggtttcag
     6421 gcctgctttt tcggcggggc tgcctttttc cacgccaccg gcaacggttg tgattttaaa
     6481 gggcatcagt ccgatgtagc cttggttttt tgcgatttta ccggcttccg gcgtgcctgc
     6541 ggcatcgatg gtgcggacgg tttgcgcgcc tgatgccgtc tgaacgccga cggcgacttt
     6601 gccggcttcg aggttgagga cgatttcggt ttgcgcgctg ccccaatctg caacgggtgt
     6661 gccgttgacg gattgtattt tgtcgccgct ttggaagccg gcgcgggcgg caatggtgtc
     6721 gggttcgact gtgccgacat aggggcgcag ttcggttacg ccgaaggaaa agctcagtcc
     6781 gtacagcaaa accgccagtg cgagattggt cagcgggccg gcggcgacga tggcgatgcg
     6841 tttggcgggg tgttgtttgt caaaagcgta gggtaaatcg gcttctgata cttcgccttc
     6901 gcgcgtatcg accattttga cgtaaccgcc caacggaatc ggggcgaggc accattcggt
     6961 gtcgccgcgc tttcgggtga aaaacggttt gccgaagccg acggaaaagc gtacgacttt
     7021 gacgccgcac aatctggcaa cgatgtagtg tccgaactcg tgcaggctga ccaaaatcag
     7081 gatggcgaag ataaaagcta gaagggtgtg caaatggttt tcctttgata acggtgttca
     7141 gacggcatca gcgcagtgtg ccgataaatg cccgcgcttg tgcgcgtgtc cgggcatctt
     7201 gcgccaagag cccccctatg tcgcctatgc cgtctgaaaa gtcttgtgaa agacaatggg
     7261 cgacggtttt ggcaatgtcg gtaaacttaa tctgtccgtc caaaaaggcg gcgacggcgg
     7321 cttcgttggc ggcgttcaat acgcagggcg cggctccgcc tgcgttcatg gcttcatagg
     7381 cgagcttcag gcaggggaag cggtcaaagt cgggcttttg gaaggtcagc gcggacaatg
     7441 cgtcgaaatc caggtcgccg acacccgaat cgatgcgctc gggcaaaccc aaacaataag
     7501 caatgggcgt acgcatatcg ggattgccca gttgcgccaa cacggagccg tcgcggtagc
     7561 gcaccatgct gtgtatcacg gattgcggat ggatgacgac ttcgagcttg tcaggcggac
     7621 agttgaacag ccaatgcgct tcaatcagct ccaaaccttt gttcatcatg gtggcggaat
     7681 cgacggagat tttgcgcccc atgctccaat tggggtgttt gaccgcttgg gcgggcgtaa
     7741 tgctgtcgaa cgtgtttaaa tcggcggtca gaaacgggcc gccggaagcg gtcaggataa
     7801 tcgaagcgat gccgtgttcg ttcagacggc cggcgtaatc gcgcggcaaa acttggaaaa
     7861 tggcgttgtg ttcgctgtcg acgggcagca ctgccgcgcc gtttgcacgg gcggtttcca
     7921 taaacaacgc gccggaaacc accagcgtct ctttgttcgc cagataaatg gttttgcctt
     7981 tttgcgccgc tgcgagcgcg gaaggcagcc ccaccgcccc gacgatggcg cacatgacac
     8041 cgctgacttc gtcggcagag gcaacgtcaa ccaatgcctg cgcgccgtgt aaaacctgcg
     8101 tcgccgtgcc gtcgcctttc aacaaagctt caagccgggc ggcgtgttcg gcatcggcaa
     8161 cgacggcata ttcggggcgg aacgtccggc attgcaccgc cagtttctcg acctgcttat
     8221 gccctgccag cgcgaatacg cggaattttt cggggtggcg ggagacaacg tccagcgtgc
     8281 tttcgcctat gctgccggta ctgcctaata tggtcaggac ttgtggtgtc ataatgggga
     8341 taactttata ccggatgccg tctgaagcgt tttcagacgg catagaatca atttaaaacc
     8401 gacatcatcg ccgcatagac gctgataacg gcaatcaggc tgtcggtacg gtcgaacacg
     8461 ccgccgtgtc cgggcagcag gttgctgctg tctttgatgc ctgccgcgcg cttgagccag
     8521 ctttccaaaa ggtcgccgca tacgctgaca acggtcaaca ccaaaccgat taacacggta
     8581 tcgaaccagc ctgtatcgaa tgccagccag ccggcacttc gtacggcggt catgtacacg
     8641 gccacgcaaa ccgcgccgcc gattgcgcct tcccagcttt tgccagggct gattgccggt
     8701 gcgattttgt gtttaccgaa cgccttgccg ctgaaatacg cgcaaacgtc ggcaacccag
     8761 accagaccta tcacggcgag cagcggcagg gcatcatcgg gatgcgggcg cagggatacg
     8821 agcgcgaacc aaaacggcat gaccagaagc caaccgacgg cataaacctg ccaaccgccg
     8881 ttgagcctcc atttgaatct caaccataaa ggcatgatgg cgagccaaaa tgccaaaaca
     8941 acataccaaa ccaaattagg cagcatccag ccgcccgcat aggcaaccac gccgaaaacc
     9001 aaggttgcgg cgaggtaatg gttggtttta attttgcaca aaccgctcat acgggcatat
     9061 tcccacaagg caatcagggc aatcagtccg caaaatgcag cccacaacca ttgcggcgcg
     9121 taaaacagca tgcccagcat cagcggcagc agccacatag cagttatcac ccgttgtttc
     9181 agcatattca gttcctttgc tgttcgatag gcagttgctc ggaggtgcgt ccgaaccgcc
     9241 gttcgcgttt ttggaacgaa gcgacggcat cgtccaaagc cttgccgtca aaatcgggcc
     9301 acaaaatatc ggtgaaatac agttctgcat atgccatctg ccagagcagg aaattgctga
     9361 tgcgcgtttc gccgccggtg cggatgaaca aatccggttc cggtgcatcg cccagcatca
     9421 agtgtttcgc cagcgtgtct tccgtaatct cggatacgcc ttcggcaatc agtttgtttg
     9481 ccgcctgcaa aatatcccaa cgtccgccgt aatcggcggc aatgctcagg gtcaggccgg
     9541 tattgtttgc cgtcaaggct tccgcctctt cgatgccttg cagaatctgc cgattaaagc
     9601 gttcgcggct gcccagtatt tttaggcgca tattgttttc gtgcagacgg cgtacctgtt
     9661 tttgcaaagc ctgtaaaaac agccccatca ggaacgaaac ttcgtcttcg gggcggcgcc
     9721 agttttcggt tgaaaaggca aacacggtca gatattgcac acccagtttg gcgcaatgct
     9781 tcaccatatt ttccaatgcg tccaaaccgc gtttgtgtcc cattatgcgc gggaggaaac
     9841 gttttttcgc ccaacggccg ttgccgtcca taatcacggc gatatgcttg ggaatggcgg
     9901 tgtgttccaa aacggcctgc gtgctgcttt tcatgtctgc ctttcgcggt tcggcattca
     9961 aatgccgtct gaacgccgaa ccgtgcaggt taaattgcca tcaaatcttc ttctttggca
    10021 gtcaggagtt tgtcggcttc ggtaatgtat ttgtcggtca gtttttgaac cgcttcttcg
    10081 ccgcgacgtg cctcgtcttc ggaaatttct ttgtctttga ggagtttttt gatgtggtcg
    10141 ttggcatcgc ggcgcacgtt gcggatagag acgcggcctt cttccgcttc gccgcgtacg
    10201 actttaatca ggtctttgcg gcgttcctcg gtcagcatgg gcatcggcac gcggatcagg
    10261 tcgccgacag ctgccgggtt cagccccaag tttgaatcgc ggatggcttt ctcgactttg
    10321 gccgccatat tgccctcaaa cggtttcacg ccgatggtgc acgcgtccag aagcgttacg
    10381 ttggcaactt ggctgacggg gaccatgctg ccccagtatt cgacttccac ttggtcgagc
    10441 aggccggtat gcgcgcggcc ggtacgcact ttcgccagat tttctttcag tacttcgacc
    10501 gaacgctgca ttttgccttc ggctgttttt tgaatatcgt tgatcatatt gttctttcgg
    10561 tgggataagg tgggcgggca gccgtctgaa acgcgcttca aacctttcag acggcatgaa
    10621 aactgttaac tgcgaatagt accgttattc gggcatgacg acaaggcagg aggattggag
    10681 atgccgtctg aagcggcagg cgtttcagac ggcatcgtgt ccgaccgtca gccgtgttcc
    10741 cgtgtttcaa gcaggctttg gcgcaggtgt tggcgttcgt gggcatccag ccatttgcgg
    10801 cgggtgcgtt gcagcaggat gacgagggcg gaaatttcct gacgcatatt ggtgctgagc
    10861 cagaggaagc cctgccattg gtagtggagg tgttcggcga gggcttccag ttcggggttg
    10921 atggcggtgt cgatgcggat gcggcgggcg tgtctgccgt tgataagggc gacggtttgt
    10981 tgcaggtcgg tttggagcag tgtgaagtgg cggtcgagca gccggatttc gctgccgttg
    11041 agtttgggag attgcagctt ggcggcagtg gtcaggagca gctcggtggt gttgacaatt
    11101 ttacggtggg cgtgctgcat ggcttccatc atggcggggc tgatgcggct ttcgcccgat
    11161 gtggcggcga ggtggctgcg gcttttgacc atgcgtgcgt tgatttggcg cattttaacc
    11221 atattctgct ccaaacgttc gcgcgtcata cgcctgccgt tgctgatttc ggcaatcatt
    11281 ttgctgcagt cggtcaggtt gtcggcaagc atgaaacgcc acatcagtgt ggatttcagc
    11341 ggcagcagtt tggcggcggc aatggcgatg gccgcgccga tgaggacgtt catggcgcgc
    11401 atcaggccgc tgtcgaacca ttcgctgccg ttgtcgccga tgagcatgca catcgtcagc
    11461 cccgccagca tagggacgta gccgtttttg ccgaccgccg cccagccggc cagtgcgctt
    11521 gccgtgccga cggtgaggta gaagaggagg ttgccgtgga aataatgctg gttcagccat
    11581 aaaacgccca aacccgcgcc cagcccgatg accgtgccga gcatacgttc caccgccttg
    11641 gagtaaatcg ccccttgaaa ctggagcatg ccgaggacga cgaagacggt catccctatc
    11701 cactcgccgt gttggaggtg gagcagccgg gcggaggcgg tggcgaacag gacggccccg
    11761 ccgagccgga cggcgtggat gaggcggcgg tagcggtagc gttcgtagga gttgagccag
    11821 cggctgacga ggcggttgcg ttgcgaggtg ttcatatcgg ttgtgccgtc tgaagcggaa
    11881 atgtgaaaaa gcacaggctt cccgaggaag ggagggtctg tgcttggtat tggtgccgga
    11941 gaagggaatc gaacccccga ccttcgcgtt acgaatgcgc tgctctaccg actgagctac
    12001 accggcgttt tttcgtcatg atatatatga acggttgttt gtgcaacttt tcgggcgggc
    12061 ggcaaggcag tgcgcg
//
LOCUS       DAXHMX010000056        12013 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHMX010000056 DAXHMX010000000
VERSION     DAXHMX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.056"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(50..2350)
                     /gene="recQ"
                     /locus_tag="ACFM4S_001086"
     CDS             complement(50..2350)
                     /gene="recQ"
                     /locus_tag="ACFM4S_001086"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6050783.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSSTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     TREDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLHERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSNKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNAADFSHDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKHKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
     gene            complement(2415..3197)
                     /gene="trpC"
                     /locus_tag="ACFM4S_001087"
     CDS             complement(2415..3197)
                     /gene="trpC"
                     /locus_tag="ACFM4S_001087"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6050784.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRAGDIEAEVGKLF"
     gene            complement(3247..4203)
                     /locus_tag="ACFM4S_001088"
     CDS             complement(3247..4203)
                     /locus_tag="ACFM4S_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC6050785.1"
                     /translation="MILTPPDTPLFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRDRGWHGVVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTFAARYREIYAVGVSLGGNALAKYLGEQGKKALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKAKSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPIPDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            complement(4336..5874)
                     /gene="murJ"
                     /locus_tag="ACFM4S_001089"
     CDS             complement(4336..5874)
                     /gene="murJ"
                     /locus_tag="ACFM4S_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6050786.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEAAEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAQDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRKHGIYRPGKGWAAFLAKMLL
                     ALAVMGGGLYAAQIWLPFDWTHTGGMQKAARLFILIVIGGCLYFASLAALGFRPRHFK
                     RVES"
     gene            complement(5874..6065)
                     /locus_tag="ACFM4S_001090"
     CDS             complement(5874..6065)
                     /locus_tag="ACFM4S_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050787.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGRFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            6092..6790
                     /locus_tag="ACFM4S_001091"
     CDS             6092..6790
                     /locus_tag="ACFM4S_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6050788.1"
                     /translation="MKSRQLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(6847..7851)
                     /gene="amgK"
                     /locus_tag="ACFM4S_001092"
     CDS             complement(6847..7851)
                     /gene="amgK"
                     /locus_tag="ACFM4S_001092"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6050789.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDFGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            7921..10329
                     /locus_tag="ACFM4S_001093"
     CDS             7921..10329
                     /locus_tag="ACFM4S_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6050790.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMEIEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            10431..11477
                     /locus_tag="ACFM4S_001094"
     CDS             10431..11477
                     /locus_tag="ACFM4S_001094"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6050791.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTASAKVAKVAKVAKVAK
                     VAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKE
                     AQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRD
                     FADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKA
                     DSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFE
                     EAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVN
                     LLRDLHSGAYVDIR"
BASE COUNT         2771 a         3445 c         3366 g         2431 t
ORIGIN      
        1 caggaatttt taaatagagg caatgccgtc tgaaacttgg taacgggctt cagacggcat
       61 ttcgttccaa taccgccaac accgccgcac cgtaacgtgc ggctttttct tcgcctacgc
      121 catatacggc ggcaagctcc gccaaacctt ccggctgttt ggcggcaatg gcgcgcaggg
      181 cggctttgct gagaatgcgg tagggttcgg actgttcgtg ttttgccgtt tcgccgcacc
      241 attgaatcag ggcgcgcatc aggcggcgtt tgtgtttggc ggcttcatcg atgccgtctg
      301 aaaacggacg gcagacggcg aggatgtccc gtccgtattt ggcggcgcgt acgctgccca
      361 agccgtgcac gccttcgagg tcggtttcgg tttcgggcgt ggcggcaagc atatcggcaa
      421 ggctttcgtc ggagaggacg gcatgcaggg cgcagttttc cgcccttgcc tgttcatacc
      481 gccaggcttc gagtttttga cgcagttgtt gttcgcgttc ggtttgcgga cggatgacgg
      541 catcgtggct aaagtcggcg gcgttgcggc agacttcgag gataccgtgt ccgaaacggt
      601 cgattttggc ttcgcccaag ccgtagatgt cgtgcagacc gttgaggtct tgcggcattt
      661 tttcgacaag gtcgcgcagg gttttgtcgc cgaaaatcat ataggcgggg atgccttcgg
      721 cttctgcctg tttcatacgc caaacgcgca atgcctgcca caggcgttct tcgcgttcgg
      781 tacgcagcca gttgtctttg agggtgcggg cggcgggctt gtcgcgcttg agcggacgca
      841 gcatcacttc ggtttcgcct ttgaggactt ttttggcggc ttcggtcagt tgcaatgcct
      901 gatatcgggt aatgttgacg gtgaggtagc cgaggctgat acactggcgg atgacgctgc
      961 gccattcttt gttggacaac tccgtaccga tgccgaatgt ggacagttgt tcgtgccggt
     1021 tgccgcgtat ccaatcgtcg cttttacctc gcaaaaggtt ggtgatgtaa ccggcggcaa
     1081 aacgttgtcc ggcgcggtac acgcagctga gtaatttttg caccaacacc gtgccgtcaa
     1141 accgtacggg cggatgcagg cagttgtcgc aatggccgca gggttcggat gcttcgccga
     1201 aatgtttgag cagcagtacg cggcggcagg cggcggtttc gcagacggca agcatggcat
     1261 cgagtttttg catttcgatt tgcttttgca cctcgtcgct gttgccttcg gcaatccgtt
     1321 cgtgcagcaa cacccaatcg ttcaaaccgt aacacagcca actcacggca ggcagcccgt
     1381 cccgtccggc gcgccccgat tcctgataga aatgttcgac actctggggc atatcgagat
     1441 gggcgacaaa gcgcacgtcg ggtttgtcta tgcccatacc gaacgccacg gtcgccacca
     1501 cgataatatt gtcttcacgc gtaaagcggc gttggttttc ctcgcgcacg tccatgctca
     1561 aaccggcatg atacggaatc gcgtttaatc cgttttcacg caaaaactgc gccacatctt
     1621 cgactttttt gcggcttagg caatacacaa tgccgctttg ccccgtcatt tctttgcgga
     1681 tgaattcaag cagttgtttt ttgccattat ttttttcgat aacctgataa taaatattcg
     1741 ggcggtcaaa gctggagata aattcgggcg catcgtccaa gtgcagataa tgcttgatgt
     1801 cggcgcgcgt ggcggcatcg gcggtggcgg tcagggcgat gcgcgggacg ttcggatagc
     1861 gttcggcaag catgccgagc tgttggtact cggggcggaa atcgtgtccc cattggctga
     1921 cgcaatgcgc ctcatcaatg gcgaacaggc tgacggtttg ttggtcgaga aaacgcaaaa
     1981 agcggtcggt aaccaagcgt tccggcgcga cataaagcag tttcagacgg ccttgggcaa
     2041 gcttgtcggc aatctcgcgc gccccatctg ccgatgtgct gctgttgact gccgccgctt
     2101 cgatgccggc ggcgtgcagg tttgccactt ggtcgttcat caacgcaatc agcggcgata
     2161 cgacaaccgc cacgccttcg cgcatcagcg cgggaatctg gtaacacaaa gacttgccgc
     2221 cgcccgtcgg catcagcacc gtcaaactcc cgccgcctgc caaagtattg atgacagcct
     2281 cctgcctgcc gcgaaattcg ggataaccaa atacttcgtg cagaatctgt ttggcggtcg
     2341 gtcggtgcat gatggttccg tgctcggtaa gggtgttggt cggttggcgg caatatgccg
     2401 tctgaaatcg ggatttagaa gagtttgccc acttccgcct caatatcgcc ggcacgcatg
     2461 aacgtttcgc cgatcaggaa ggtgtgcaca ccgcgcgatt gcataaattc cacatccgcc
     2521 ttgcctgtaa tgccgctttc ggttacgacg gttttgcctt ccagcgcggg cagcagcgac
     2581 agggtttggt cgagggagac ttcaaaagtc ctcaggttgc ggttgtttac gccccacaac
     2641 ggcgtggtca ggttgcggca tttttccaat tcggtttcat cgtgcagctc gagcaggacg
     2701 gtcatgccca attcgtgcgc caccgcttca aagcgttcca attgttcctg ttccagtgct
     2761 gcggcaatca gcaggacggc atccgccccc catgcgcgcg cctgataaac ctggtattcg
     2821 tcgatgatga agtctttgcg cagcacgggc agcgatacgg cttcgcgcgc ctgtttgagg
     2881 tattcgggcg aaccttggaa atagggttcg tcggtcagta cggacaaaca cgccgctccg
     2941 gcgttttcat aggcgcgtgc aatctcggca gggcggaagt ccggacggat taaccctttg
     3001 ctcgggcttg cctttttgat ttcggctatg acggcgggca ggtttaggcg gtgtttgccg
     3061 cgtatcgaat cgatgaagct gcggacgggc gcggcttctg cggcaagtgc gcggatgtgt
     3121 tcggcgttga cggcggcttt ttgagcggca acttcctgtg ctttggtggc aaggatttta
     3181 ttgaggatgt cggtcatgtc gggttccgta ttcgtctggg aaagggggaa tattagcatc
     3241 aaaccgttaa cgactgtttg tgcggaagct gtcgaaatag gacaggacgg tctgcggcaa
     3301 ccattgcagg tgcagcctgc cgccggtgct gccgacaaag ccgacatgac caccgtgtgc
     3361 aggttggaac agggtaacgg cttcggacac ttcgtccggt atcggcaggg cttcgggcgg
     3421 caggaagggg tcgttgacgg cattgagcag gagcagcggt ttggcaacgt gtttgagcag
     3481 cggtttgcag gaagtttggc ggtagtagtc gtgccggtcg gcaaagccgt gcagcggtgc
     3541 ggtgaagcgg tcgtcaaact cgcccagtgt tttgcaccct gcggcaaatg ccgtctgaaa
     3601 accttggagc gattttgctt tgggtatcag ggtgcggagg aagtagcgcg tgtagagcag
     3661 ccgcgtgatg ccgctgtcga agcgtctgcc tgccgcctct gcatcgacgg gggcggagat
     3721 gacggcagcg gcttgcggca atgccttttt gccctgttcg cccaaatatt ttgccagcgc
     3781 gttgccgccc agcgatacgc cgacggcgta tatttcacgg taacgcgcgg cgaacgtgtc
     3841 caaagtaaag gcgatttcgg cggtatcgcc caagtggtag aacaccggag cggtgttggc
     3901 aatgccgccg cagctgcgga aatggacgac tacgccgtgc caaccccgat cgcgtaccgc
     3961 aagcatcagt tcgaccgcgt aatggctgcg gctgcttcct tccaaaccgt gaaacagcac
     4021 gaccagcggc gcatcgggcg aaatgccgtc tgaaaagtcg taggcgactt tggttttacc
     4081 cgtgctgtcg ggaagcagct cgcggcggta tgcaggtgcg gggcgttgca ggaatttggc
     4141 ggcaatcgtg tcggcattgc cgttgcggag gaaaaggggc gtgtccggcg gtgtcaaaat
     4201 cataaggtat cggttttctt gttttcagac ggcattgatg atgcggcagc ccgtccggcc
     4261 ggtgcggacg cgggggatgc gcgcccgaat ataggcgtgg aaaaagcgtt tgccgaaaaa
     4321 ggatatcggc atcggtcagc tttccacccg tttgaaatgg cgcggacgga agcccagtgc
     4381 agccagtgat gcgaaataca ggcagcctcc gatgacaatc aggatgaaga gccgggcggc
     4441 cttttgcatt ccgccggtgt gtgtccagtc gaacggcagc cagatttggg cggcatacag
     4501 gccgcctccc atcacggcga gcgcgagcag catttttgcc aagaacgccg cccaaccctt
     4561 gccgggccgg taaataccat gtttgcgcaa caggtaaaac aacaatccgg cattgataca
     4621 cgcgcccaga ccgatggcaa gcgaaagtcc gacgtgtttc agtgggccga taaaggcaag
     4681 gttcatcaac tgcgtgcaaa tgagcgtgaa gatggcgatt ttgacgggcg ttttgatgtt
     4741 ttgccgcgca taaaagccgg gcgccaacac tttaatcatg attaaaccga ttaaaccgaa
     4801 agaataggca atcagcgcgt gttgcgtcat ttgcgcgtca aacagcgtga attcgcggta
     4861 cataaacagc gtcgccacca gcgggaacga caacaccgcc agtccgaccg ccgccggcag
     4921 cgtcagcagc atacacaggc gcaaacccca gtcgagcagg gcggaaaact gttccgtatc
     4981 ttggtttgcc gagtgtttgg acaaagtcgg cagcaaaatc gtaccgagtg ccgcccccag
     5041 cacgccgccg ggcagttcca tcatgcggtc ggcgtaatac atccatgaaa cgctgcccga
     5101 ttgcagataa gacgcgaaaa tcgtgttgat caccaaagaa acctgcgcca cgctcacgcc
     5161 caaaatcgcc ggcgccatct gtttcatcac gcggttgacc gccgcatctt tgaaattcag
     5221 tttgggcagt ttcaaaaagc ccagtttcgc cagccagggc agttggaagc cgagttgcaa
     5281 aataccgccg acaaaaaccg cccatgccag agcggtaacg ggcggatcga aatacggcac
     5341 gaaaaacagc gcgaatacga taaacgacac gttcaggaat gtgggcgtaa acgcgggaat
     5401 gccgaactta tgataggaat tgagtaccga gccgacaaaa gaggaaagtg aaatcaatag
     5461 gatataagga aacgtaatcc gcagcaaatc gatggaaagt tggaacttgt ccgcgtcttg
     5521 ggcaaagccg ggtgcggaaa cataaatcac ccaaggcgcg gcgagtatgc ccagcgcggt
     5581 aacgatgacc agcacaaacg acagcatccc cgccacatgg cggataaaag cctccgccgc
     5641 ctctttagaa cgcgtttcct tatattccgc caaaatcggc acaaacgctt gggcaaacgc
     5701 cccctccgca aacacgcggc gcagcaggtt gggcagtttg aacgcgacaa aaaacgcatc
     5761 cgtcgccata cccgcgccga atgcccgcgc aatgaccgta tcgcgcacaa atcccaaaac
     5821 gcgcgacacc atcgtcaggc tgccgacttt tgccaaagct cccagcatat tcatcattgt
     5881 tcctcaacag tcgtacccgt ttggggcaac ggcgcgtatt gtacgacaga aaccgcttca
     5941 gacggcatcg ggtttgatgc cgtctgaagc ggtttcccga aacgaaaacg tccttttccg
     6001 gcggcaaact gtatcaacac gcggaaatgc aataaaatag ccggattccg attgatttcc
     6061 aacatctgtt tccaacatca cggagagccg tatgaaatcc agacaactcg ccctcggcgt
     6121 tgccgccctg ttcgcccttg ccgcgtgcga cagcaaagtc caaaccagcg tccccgccga
     6181 cagcgcgcct gccgcttcgg cagccgccgc cccggcaggg ctggtcgaag ggcaaaacta
     6241 taccgtcctt gccaacccga ttccccaaca gcaggcaggc aaagtcgaag tccttgagtt
     6301 tttcggctat ttctgtccgc actgcgccca cctcgaacct gttttgagca aacacgccaa
     6361 gtcttttaaa gacgatatgt acctgcgtac cgagcatgtc gtttggcaaa aagaaatgct
     6421 gccgctggca cgcctcgccg ccgccgtcga tatggctgcc gccgacagca aagatgtggc
     6481 gaacagccat attttcgatg cgatggtcaa ccaaaaaatc aagctgcaag agccggaagt
     6541 cctcaaaaaa tggctgggcg aacaaaccgc ctttgacggc aaaaaagtcc ttgccgccta
     6601 cgagtccccc gaaagccagg cgcgcgccga caaaatgcag gagctgaccg aaaccttcca
     6661 aatcgacggt acgcccacgg ttatcgtcgg cggtaaatat aaagttgaat ttgccgactg
     6721 ggagtccggt atgaacacca tcgacctttt ggcggacaaa gtacgcgaag aacaaaaagc
     6781 cgcgcagtaa gcccgtttga aaaatgccgt ctgaaacttg gttttcagac ggcattttga
     6841 ttgggtttaa aacgtaaagc ccgtttccag ttcttcatcg ccgaccagtt cgaccaagag
     6901 cgcgtagagc ggggcgagtt cggcataacg gcgcgatacg cggcgcaggt aattgaggaa
     6961 acgcgggatt tcgggacggt atttgtcttt gccgtcgcgg tagtacaggc gtgcgaagat
     7021 gcctgcaacc ttcaagtgcc gctgcacgcc catccattcg aaccagcggt aaaactcgtc
     7081 aaacgcttcg gggacgggca ggccggcagc ccgcgccttt tcccagtagc ggataaccaa
     7141 gtccaagaca aattcttctt cccattcgat aaaggcatcg cgcaacagcg acaccaaatc
     7201 gtaggaaatc gggccgtaaa gcgcgtcttg gaagtctaaa acgcccggcc tgccgcgcgt
     7261 cagcatcagg ttgcggacga taaagtcgcg gtgcacatag actttgggct gcgccaacag
     7321 gggcggcagc agcgtatcga cggtttgctg ccaaagttgg cgttgtttga atgttaattc
     7381 gcgccccaat tcttttgcga caaaccattc cgggaacagg ttgatttcgc gcaacatcgt
     7441 ttcacggtca tattcgggca aaaccccttc acggctcgcc ttctgcaatt cgaccaactc
     7501 gccgattgcc tccaaaagca gggctttgtg cgccgtttcg ccctgttcct gaagcattgc
     7561 ggtcaaaaac gtcgtattgc caaagtcgtt caataccaca aaccccagat ccgtgtccgc
     7621 gtgcaatacc tgcggcacat tgaccatgtc aaacagtttc tgcactttca aataaggtgc
     7681 gacactcatc ttgtcgggcg gtgcatccat gcagacgaca ctgctgccgt ctgaaaacgt
     7741 tgcacggaaa tagcggcgga aatcagcatc cgccgccgca aaagtcagat cgaagtcccg
     7801 ttcgggataa acggtctgaa gccaattttt cagttcggtt tgccgttgca taacagtact
     7861 aaagcatttc aggttacaat aaacgctatt ctaactggca aaccgacttg aggggcgatt
     7921 ttggctcgtt tattttcact caaaccactg gtgctggcat tgggcctctg cttcggcacg
     7981 cattgcgccg ccgccgatgc cgttgcggcg gaggaaacgg acaatccgac cgccggagga
     8041 agcgttcgga gcgtgtccga acccatacag cctaccagcc tgagcctcgg ttcgacctgc
     8101 ctgttttgca gtaacgaaag cggcagcccc gagagaaccg aagccgccgt ccgaggcagc
     8161 ggcgaagcat ccatccccga agactatacg cgcattgttg ccgacaaggt ggaaggacag
     8221 tcgcaggtgc aggtgcgtgc cgaaggcaac gtcgtcgtcg aacgcaaccg gacgaccctc
     8281 aataccgatt gggcggatta cgaccagtcg ggcgacaccg ttaccgcagg cgaccggttc
     8341 gccctccaac aggacggtac gctgattcgg ggcgaaaccc tgacctacaa tctcgagcag
     8401 cagaccggcg aagcgcacaa cgtccgcatg gaaatcgaac aaggcggacg gcggctgcaa
     8461 agcgtcagcc gcaccgccga aatgttgggc gaagggcatt acaaactgac ggaaacccaa
     8521 ttcaacacct gttccgccgg cgatgccggc tggtatgtca aggcagcctc tgtcgaagcc
     8581 gatcgggaaa aaggcatagg cgttgccaaa cacgccgcct tcgtgttcgg cggcgttccc
     8641 cttttctata cgccttgggc ggacttcccg cttgacggca accgcaaaag cggcctgctt
     8701 gttccctcac tgtccgccgg ttcggacggc gtttcccttt ccgttcccta ttatttcaac
     8761 cttgccccca atctcgatgc cacgttcgcg cccggcgtga tcggcgaacg cggcgcggtc
     8821 tttgacgggc aggtacgcta cctgcggccg gattatgccg gccagtccga cctgacctgg
     8881 ctgccgcacg acaaaaaaag cggcaggaat aaccgctatc aggcgaaatg gcagcatcgg
     8941 cacgacattt ccgacacgct tcaggcgggt gtcgatttca accaagtctc cgacagcggc
     9001 tactaccgcg acttttacgg caacaaagaa atcgccggca acgtcaacct caaccgccgt
     9061 gtatggctgg attatggcgg cagggcggcg ggcggcagcc tgaatgccgg cctttcggtt
     9121 ctgaaatacc agacgctggc aaaccaaagc ggctacaaag acaaaccgta tgccctgatg
     9181 ccgcgccttt ccgccgattg gcgcaaaaac accggcaggg cgcaaatcgg cgtgtccgca
     9241 caatttaccc gcttcagcca cgacagccgc caagacggca gccgcctcgt cgtctatccc
     9301 gacatcaaat gggatttcag caacagctgg ggttacgtcc gtcccaaact cggactgcac
     9361 gccacctatt acagcctcaa ccgcttcggc agccaagaag cccgacgcgt cagccgcact
     9421 ctgcccattg tcaacatcga cagcggcgca acttttgagc ggaatacgcg gatgttcggc
     9481 ggcggagtcc tgcaaaccct cgagccgcgc ctgttctaca actatattcc tgccaaatcc
     9541 caaaacgacc tgcccaattt cgattcgtcg gaaagcagct tcggctacgg gcagctcttt
     9601 cgcgaaaacc tctattacgg caacgacagg attaacaccg caaacagcct ttccgccgcc
     9661 gtgcaaagcc gtattttgga cggcgcgacg ggggaagagc gtttccgcgc cggcatcggt
     9721 cagaaattct atttcaagga tgatgcggtg atgcttgacg gcagcgtcgg caaaaaaccg
     9781 cgcaaccgtt ccgactgggt ggcatttgcc tccggcagca tcggcagccg cttcatcctc
     9841 gacagcagca tccactacaa ccaaaacgac aaacgcgccg agaactacgc cgtcggtgca
     9901 agctaccgtc ccgcacaggg caaagtgctg aacgcccgct acaaatacgg gcgcaacgaa
     9961 aaaatctacc tgaagtccga cggttcctat ttttacgaca aactcagcca gctcgacctg
    10021 tccgcacaat ggccgctgac gcgcaacctg tcggccgtcg tccgttacaa ctacggtttt
    10081 gaagccaaaa aaccgataga ggtgctggcg ggtgcggaat acaaaagcag ttgcggctgc
    10141 tggggcgcgg gcgtgtacgc ccaacgctac gttaccggcg aaaacaccta caaaaacgct
    10201 gtctttttct cacttcagtt gaaagacctc agcagtgtcg gcagaaaccc cgcagacagg
    10261 atggatgtcg ccgttcccgg ctacatcccc gcccactctc tttccgccgg acgcaacaaa
    10321 cgaccctgac cgtcggaaac ccggcaggag caccgttccc gcacaagacg gcattccacc
    10381 gacaacccca aacccgccat caaaggtagg attcaaacga taaggaaaga atgatgaaaa
    10441 tcaaagccct gatgattgcc gccgcattgc tggcagcagc cgatgtccac gccgcaccgc
    10501 aaaaggcaaa aaccgcatcc gccaaagttg ccaaagttgc caaagttgcc aaagttgcca
    10561 aagttgccaa agttgccaaa gttgccgcca cggcgcaaaa agaagccgca cccgcacaac
    10621 agcagggcgg tatccgcttt tcagacggca ttgccgccgt tgccgacaac gaagtcatca
    10681 cgcgccgccg gcttgccgaa gccgttgccg aagccaaagc caacctgccc aaagaggcgc
    10741 agataagcga atccgagctg tcccgacagg tgctgatgca gcttgtcaac caatccctga
    10801 ttgtacaggc gggcaaacgc cgcaacattc aagcaagcga agcggaaatc gatgccgtcg
    10861 tcgcaaaaaa ttccgccctc aaaaacctca gccccgccca acgccgcgat tttgccgaca
    10921 acatcattgc cgaaaaagtc cgccagcagg cagtgatgca gaacagccgc gtgagcgaag
    10981 ctgaaatcga tgccttcctc gagcaggcgc aaaaacaagg catcaccctg cccgaaggcg
    11041 caccgttgcg ccaataccgc gcccaacaca tcctgattaa agccgacagc gaaaacgccg
    11101 ccgtcggcgc ggaaagcacc atccgcaaaa tctacggaga ggcccgcagc ggcacagact
    11161 tttccagcct agcgcgccaa tattcgcaag acgcgagcgc gggcaacggc ggagatttgg
    11221 gctggtttgc cgacggcgtg atggttcccg cctttgaaga agccgtccac gcgctcaaac
    11281 ccggacaggt cggcgcgccc gtccgcaccc aattcggctg gcatatcatc aaattgaacg
    11341 aagtgcgcga tgccggcaca cctcaggaac gtatccgcaa ttccgtgcgg caatacatct
    11401 tccaacaaaa agccgaacag gcaaccgtca acctgttgcg tgacctgcat tccggcgcgt
    11461 atgtcgacat ccgctaaggc ggtttgaagc aaaaagccat accgatcggc aaaaatccgg
    11521 gcggtatggc tttttggatt tcgagttact tttacaccgt cattcatcat tcccgcgaaa
    11581 gcgggaatct agaaacgaaa agtaacagga atttatcggg aatggctgga gtttaaagga
    11641 ctggattccc gccgtcgcgg gaatgacggg attttgggtt gtggtaattt atcggaaaaa
    11701 caaaaaaacc tatgccgtca ttcccgagca ggcgggaatc cggttattta aaactgcaga
    11761 aatttatccg aagcaacaac aatctttcca tcgtcattcc cgcgtaggcg ggaatctagg
    11821 acgtagaatc taaagaaacc gttttatccg ataagtttct gtaccgaaga atctggattc
    11881 ccgctttcgc gggaatgacg gcgcataagt tcccgtgcgg acagacctag attcccacct
    11941 gcgtgggaat gacgattcag aagttgcctg aaacctaaaa aactgaaacc gaacgagccg
    12001 gatttccgct ttc
//
LOCUS       DAXHMX010000057        11744 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHMX010000057 DAXHMX010000000
VERSION     DAXHMX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.057"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(354..776)
                     /locus_tag="ACFM4S_001095"
     CDS             complement(354..776)
                     /locus_tag="ACFM4S_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6050792.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(894..1565)
                     /locus_tag="ACFM4S_001096"
     CDS             complement(894..1565)
                     /locus_tag="ACFM4S_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6050793.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1581..2207)
                     /gene="purN"
                     /locus_tag="ACFM4S_001097"
     CDS             complement(1581..2207)
                     /gene="purN"
                     /locus_tag="ACFM4S_001097"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6050794.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2314..3132)
                     /locus_tag="ACFM4S_001098"
     CDS             complement(2314..3132)
                     /locus_tag="ACFM4S_001098"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6050795.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3391..3831)
                     /locus_tag="ACFM4S_001099"
     CDS             complement(3391..3831)
                     /locus_tag="ACFM4S_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6050796.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3894..5300)
                     /locus_tag="ACFM4S_001100"
     CDS             complement(3894..5300)
                     /locus_tag="ACFM4S_001100"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6050797.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5470..6585
                     /gene="lptF"
                     /locus_tag="ACFM4S_001101"
     CDS             5470..6585
                     /gene="lptF"
                     /locus_tag="ACFM4S_001101"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6050798.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6582..7652
                     /gene="lptG"
                     /locus_tag="ACFM4S_001102"
     CDS             6582..7652
                     /gene="lptG"
                     /locus_tag="ACFM4S_001102"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6050799.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7952..10537
                     /gene="acnB"
                     /locus_tag="ACFM4S_001103"
     CDS             7952..10537
                     /gene="acnB"
                     /locus_tag="ACFM4S_001103"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6050800.1"
                     /translation="MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10679..11674
                     /locus_tag="ACFM4S_001104"
     CDS             10679..11674
                     /locus_tag="ACFM4S_001104"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6050801.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2692 a         3264 c         3056 g         2732 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta taattcaata aattaatata
       61 tggcttaaaa taacgggatg cccgcctccc gtcctcctgc agaagcgggc gggtatcatt
      121 ttaaaacgcg gcatttaaaa tttgaccgaa aattgttgac aatccggaat caagtctgca
      181 caataccccg acaagtccaa gtattataaa ggctgaataa agaggaaaca gcaggcagat
      241 atattcggga ggtgcagtcc gaatatatct gcttttttat gcgcctccgg attgcctgcc
      301 gcacctttcc cttcagacgg catcagccgt ttccccataa tgccgcccga tgcctattta
      361 tctgccccgg caatttcaaa actgtgggta atctttgccg ctttgcccaa cataatcgaa
      421 gccgaacagt atttttcggc agacatctga acggcgcgct caatggccga ttctttcaaa
      481 tcatgcccga atactttgaa atggatgtgg atttcggtaa acacgcgcgg cgcatcgtcc
      541 gcccgtttcg ccgtaaccgt cgcacggcag tcagtcactt tctgacgctg tttttcggca
      601 atcatcacca catcgatgct cgaacagccc gccacgccca acagcagcat ttccaaaggg
      661 ctgggcccgc gcttagcctt accttctgcc gccgacccct ccataacgac gctgtgcccg
      721 ccttccgttg ttccgacaaa gcacattccg tctatccatt ttgatgtaac ctgcatggtg
      781 tcattcctga aaatagcgtt aaaaccgctt tgcatatggc gttattgtaa acaatttcaa
      841 gcggcttatg cagaaatatg gacaaaacgg caaaaaaacg cctgaaaatc gatttacggt
      901 ttggcggact ggccattgat ctgcaccgat ttgagtttca gcgtataggt tttgccgtcg
      961 tcggtatagc cgatttgtgc cggaatattg ttcagggacg gtgcgaagaa atacattacc
     1021 gtatcgtcgc cgcgccgcac ccgatatttg acgacttcgg tttccacgcc gcctatgctg
     1081 tattttcctg tacccgcctt attcaaaccg ccgacggaat aaagtttttt gccgttggtg
     1141 attttcagcc ccggggggag tttcgcgtca tttgccgcca actgccaggc aagcgtgaac
     1201 aaatccatag ccttggggct ttgctcggtt ttgctctcgc ccgctttgcc gtaggttacg
     1261 ctgccgtcgg cgaatttggc ttccgcatac agtttgcccc tgcgtatgtc tctatagtag
     1321 gtagggtgca gggtattgcc gacaaccgta ccgccggact cgaaacggat attgtatagc
     1381 ggcactttaa tcgtcgaaac gattttgtaa gcattgccgc tgcgttcaaa tgtcatcgtg
     1441 gcgggaatgc cgtagctgcc ggaatagtgc agcacggcgg attggggcag ccctgccgca
     1501 tacgcgcacg gcagggcggc ggacaaaatg gcggcggaaa atatattttt aaaagtcttc
     1561 atcatttgct cccgcccggt ttacgccgtc aggaaacggg cggcatcggc gttttccgaa
     1621 tttctgacgc ggtttccctc gataatcagg cggccggcgg caaaatcggc aacggctttc
     1681 ggatagagtt tgtgttcgac agccaaaacc cgggcggcaa catcgtctgc cgtatcgccg
     1741 tcgagtatcg gcacaatccc ttgcgataca atcgggccgc aatccagttc ggcagtaacg
     1801 aaatggatgg tgcagccggc aacgcggcag cccgcctcca aagcgcgttc gtgcgtatga
     1861 agtccggtaa acgagggcag gacggacggg tgaatgttca tcagcctgcc ttcgtaatgg
     1921 gcgcaaaact caggggtcag aatccgcata aaacctgcca aaaccaccaa gtcgggttga
     1981 tatgcgtcga ttttttccat catggcggta tcgaaggcaa gtcgtgattc aaagttttta
     2041 tgattcaggc tgtcggtcgg aataccctga ccggccgccc attgcaaacc ggcagccgtt
     2101 tcgctgttgc tcaacacggc ggcaatacgg acgttgggaa tggcggcatt gacgattgcc
     2161 tgcatattgc tgccgcgtcc ggaaatcagg atgacgatgt ttttcataat ggtgcgcttt
     2221 tgaaagggat gccatctgaa ccgctgtttg gtggtttcag acggcatttg ccgtaaaaat
     2281 gcccgaaaac ctgtttcggg catggattcg gacttaattt acttttttga tgtcgacttg
     2341 agccggctgc ttggcgggcg cgttttcggg tgcgccgatt ttgaccagtt tcacatcaaa
     2401 taccaaagtg gcgttcggac cgattttgtc gcccgcaccc tgttcgcggt aggcaaggtt
     2461 ggacgggatg tagaacgtgg cttcgccgcc ttctttcaga agctgtacgc cttcggtcca
     2521 acccggaatc acttggctca aagggaaggt gaccgggccg ccgttggctt tgctgctgtc
     2581 gaataccgta ccgtcaatca ggcggccttc gtattccacg gtaacgatgt cgtctttggt
     2641 cggctgtttg ccttcaccct gtttggtgat tttgtattgc aggccggaag cagtggtctt
     2701 cacgccgtct ttggcggcat tttctttcag aaaggcttcg cctttttctt tattggcctt
     2761 cgcgtccgcc ttgtgttttt ctacggcttt agcctgttgt tcctgaagga atttcatcat
     2821 gacttcctga gcctgctctt cggtcatttt gatttctttg ccgtcataca ctgcctgcat
     2881 ggcttcggta aagactttca aatcgatttc cgcgccctgt tccttcattt gcttcaggga
     2941 gcgtccgatg tccacgccca tcgcatagct tgcctgctgc atcgtgctgc cgatcgaaga
     3001 ggtgtcgccc tgcgcggaag aagcggcggc aggttcggat gcagatgcgg gggcggcttc
     3061 ttttttgccg caggcggaaa gtgccaaagc ggcggaaagg gtcagtgcgc tgattttgaa
     3121 aatggtgttc atgatggatc ttcgctgtcg ataaggtcgg aaaaacggga ttatagccga
     3181 gtttgaatgt ttcaacacac aggatgacac ataaagcgtc aatcgtgtgt tgccctgttt
     3241 tggaaaggat tgaaccttcc aaaataagtt ttgattctac cgccccgagg gacagatgtc
     3301 caagtggcgg ggttcaaccg ataaggaaat tttaatcaaa tagaatcaag cctgtttaaa
     3361 ttttgtaaat gcggcatttc agacggcatt ttatgccttg ccctccatgc cgtgatgttc
     3421 gatggcaaaa ccgcttcggc ggtaggcggt aaagcgttcg cgcgcgtcgg cgagatcttc
     3481 caagctgttg ccgacgattt ccaaaacgcg cgcgggcaag accggagcgg tgttccaaaa
     3541 accgtcggac aggttcaaaa cagtcatgcc ttcgggaatt cggggcggat tgccgccgca
     3601 agcaagcagg acggatgttt cagacggcat ggcttcttcc atttcccaaa tttcgtgcgg
     3661 aataaaactc tcggcctcgt attgccaaag cattttgtcc aattcctgaa gctgcccgaa
     3721 cgaatcggac cacaccagta tcctgccgcc gtcccgaatc gcacgggcaa tcaggcggca
     3781 ggtgaaaatc ggaacttggg caacgtgcgt gtaaaaggtg gctttcggca tattgtttga
     3841 gcatttggca ggacgatgcc gtctgaaagg cttcagacgg cattgtggca aaattaaaga
     3901 ttccgcagat agttcagcag caagggaacg ggacggccag tcgcaccttt ttccgctccg
     3961 gatttccacg ccgtacccgc gatgtcaagg tgcgcccacg ggtagccttc ggtgaagtaa
     4021 gacaggaatg ttgcggcggt aatcgtgccc gcgccgggcg tgccgatgtt tggaatgtcg
     4081 gcaaagttgg atttgagttg gtctttgtag gtctcaaaga gcggcagttg ccatgctttg
     4141 tcgtccacgt tgtaggaagc ggcaagcagg ctgtcgatca aatcctgatt gttgcccatc
     4201 acgccgctga catcgtgccc caaggcaaca atacacgcgc cggtcagggt ggcgacgtcg
     4261 atgacggctt tgggtttgaa ctgctcggcg taagtgagcg cgtcgcacaa aatcagacgg
     4321 ccttcggcat cggtgttcaa cacttcgatg gtcagacctt tcatactttt cacgacatcg
     4381 cccggtttgt ttgccgcgcc ggaaggcata ttttcacaag tggcgacgac ggcaatcagg
     4441 ttaatcggca gttgcagttt gacggcggcg cagaaggtgc tgatgacggt tgccgcgccg
     4501 cacatatcaa acttcatttc gtccatgttc aggccgggct tgagggagat gccgccggtg
     4561 tcgaaggtaa tgcctttgcc gaccaatacc acaggcgcgg cttctttgtc ggctgcaccg
     4621 aaatagctca gttcgaccaa atagggggct tccgcgctgc ctttggcgac cgaccaaaac
     4681 gaacccatgt tttctttgat gtagtctttt tcgatgattt tggcgtgcgc gcccagtttt
     4741 tcggcttcgg ctttggcggt gcgcgctaaa aattcgggcg tgcattcgtt gggcgcggcg
     4801 ttgcccaagt cgcggcagag gctttgtccg taaacttgcg cttcggcgac gcgcaaggct
     4861 tctttaacga cggcttcgtg tgcgctgtgg aacacggcgg tttcaaattt ggcgggcttg
     4921 gcttcttttt tgtagcggtc gaaacggtag gcggcgttgc cgaacgcaat cgcaaacgct
     4981 tcggctacgg cggcggcttg cgcttcatcg aaggcatgga tgtccacatt gaccgtttcc
     5041 tgattttgcg cccatttggc ggcttcggcg gcggccttgt tcaatgcggc gcggccggtg
     5101 cttttcagac agcatacggc aacagcctgc aaaccgttgc ctgtcgggat ttttgtgtcg
     5161 gcaaaatttt gaccttcttc aagcgaagac aaaagggcaa gggcggtcgg atggtttaat
     5221 tgcgcctctt cggtgcagac aaataactgt gcgcctgcct gctgttcctg caagatttcg
     5281 gtttttgtgc taaattccac gtttattctc ctgattgaga cggttgtcgg tagttttcgg
     5341 acggcctttc gctcaaaaga ccgtctgaag acggctggca cgattgtacc ccatttgaag
     5401 caccgtctga aaccttgcgc ggacaatccg cctgcgccga accgcttacc gccccctgac
     5461 cgcgattcta tgatttatca aagaaacctc atcaaagaac tctcttttac cgccgtcggc
     5521 attttcgtcg tcctcttggc ggtattggtc tccacgcagg caatcaacct gctcggccgt
     5581 gccgccgacg ggcgtgtcgc catcgatgcc gtgttggcat tggtcggctt ctgggtcatc
     5641 ggtatgacgc cgcttttgct ggtgttgacc gcatttatca gtacgttgac cgtgttgacc
     5701 cgctactggc gcgacagcga aatgtcggtc tggctatcct gcggattggc attgaaacaa
     5761 tggatacgcc cggtgatgca gtttgccgtg ccgtttgccg ttttggttgc cgtcatgcag
     5821 ctttgggtga taccgtgggc agagctacgc agccgcgaat acgctgaaat cctgaagcag
     5881 aagcaggaat tgtctttggt ggaggcaggc gggttcaaca gtttgggcaa gcgcaacggc
     5941 agggtttatt ttgtcgaaac cttcgatacc gaatccggca tcatgaaaaa cctgttcctg
     6001 cgcgaacagg acaaaaacgg cggcgacaac atcatcttcg ccaaagaagg taacttctcg
     6061 ctgaacgaca acaaacgcac gctcgaattg cgccacggct accgttacag cggcacgccc
     6121 ggacgcgccg actacaatca ggtttccttc caaaaactca acctgattat cagcaccacg
     6181 ccaaaactca tcgaccccgt ttcccaccgc cgtaccattc cgaccgccca actgattggc
     6241 agcagcaacc cgcaacatca ggcggaattg atgtggcgca tctcgctgac cgtcagcgtc
     6301 ctcctactct gcctgcttgc cgtgccgctt tcctatttca acccgcgcag cggacatacc
     6361 tacaatatct tgattgccat cggtttgttt ttaatttacc aaaacgggct gaccctgctt
     6421 tttgaagccg tggaagacgg caaaatccat ttttggctcg gactgctgcc tatgcacatc
     6481 atcatgttcg tcatcgcaat cgtacttctg cgcgtccgca gcatgcccag ccagcccttc
     6541 tggcaggcgg ttggcaaaag tctgacattg aaaggcggaa aatgaacctg atttcacgtt
     6601 acatcatccg tcaaatggcg gttatggcgg tttacgcgct ccttgccttc ctcgctttgt
     6661 acagcttttt tgaaatcctg tacgaaaccg gcaacctcgg caaaggcagt tacggcatat
     6721 gggaaatgct gggctacacc gccctcaaaa tgcccgcccg cgcctacgaa ctgattcccc
     6781 tcgccgtcct tatcggcgga ctggtctccc tcagccagct tgccgccggc agcgaactga
     6841 ccgtcatcaa agccagcggc atgagcacca aaaagctgct gttgattctg tcgcagttcg
     6901 gttttatttt tgctattgcc accgtcgcgc tcggcgaatg ggttgcgccc acactgagcc
     6961 aaaaagccga aaacatcaaa gccgccgcca tcaacggcaa aatcagcacc ggcaataccg
     7021 gcctttggct gaaagaaaaa aacagcatta tcaatgtgcg cgaaatgttg cccgaccata
     7081 cgcttttggg catcaaaatt tgggcgcgca acgataaaaa cgaattggca gaggcagtgg
     7141 aagccgattc cgccgttttg aacagcgacg gcagttggca gttgaaaaac atccgccgca
     7201 gcacgcttgg cgaagacaaa gtcgaggtct ctattgcggc tgaagaaaac tggccgattt
     7261 ccgtcaaacg caacctgatg gacgtattgc tcgtcaaacc cgaccaaatg tccgtcggcg
     7321 aactgaccac ctacatccgc cacctccaaa acaacagcca aaacacccga atctacgcca
     7381 tcgcatggtg gcgcaaattg gtttaccccg ccgcagcctg ggtgatggcg ctcgtcgcct
     7441 ttgcctttac cccgcaaacc acccgccacg gcaatatggg cttaaaactc ttcggcggca
     7501 tctgtctcgg attgctgttc caccttgccg gacggctctt cgggtttacc agccaactct
     7561 acggcatccc gcccttcctc gccggcgcac tacctaccat agccttcgcc ttgctcgccg
     7621 tttggctgat acgcaaacag gaaaaacgtt gaaccaatgc cgtctgcccc ccttcggacg
     7681 gcattggttt gtattatcat attcccgtag acagatagcc gttccctatt atagtggatt
     7741 aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta ttgtactgtc tgcggcttcg
     7801 tcgccttgtc ctgatttaaa atacattacc tgtcataaca gttccatttt tgttaaaact
     7861 agtctatgat agcggtacaa atattgttta caatatttaa cgcaaatcat ttgcaacccg
     7921 acaaaagaaa aacagaaaaa ggaacaaaga gatgttagaa gcctatcgta aagccgccgc
     7981 cgagcgcgcc gcccttggta ttcccgccct tcctctgact gctcagcaaa ccgccgattt
     8041 ggttgagctg ctgaaaagcc cgcccgcagg cgaaggcgag ttcttggtcg aactgcttgc
     8101 ccaccgtgtt ccgcccggtg tggacgatgc cgccaaagtc aaagcctcat tcctggctgc
     8161 cgttgccgaa ggcagcgcgt ccagcccgct gatctccccc gaatatgcga ccgaactctt
     8221 aggtacgatg ctcggcggtt acaatatcca cgccttaatc gaactcttgg acgacgacaa
     8281 actcgcgccc attgccgcca aaggcttgaa acatacgctt ctgatgttcg attccttcca
     8341 cgacgttcag gaaaaagccg aaaaaggcaa caaatacgcg caagaagttt tgcaatcttg
     8401 ggcagatgcc gaatggttcg cctcacgcgc caaagttccc gaaaaaatca ccgttaccgt
     8461 tttcaaagtt gacggcgaaa ccaatacaga cgacctctcc cccgcgcccg acgcgtggag
     8521 tcgtcccgat attccgctgc acgcgctggc catgctgaaa aacccgcgcg acggcatcac
     8581 gcccgacaaa ccgggcgaag tcggtccgat taaattgttg gaagaactca aagccaaagg
     8641 ccatccggtt gcttacgtcg gcgacgtggt cggtactggt tcttcacgca aatccgcgac
     8701 caactccgtc atttggcata ccggcgaaga cattccgttc gtgccgaaca aacgcttcgg
     8761 cggcgtatgt ttgggcggca aaatcgcgcc gattttcttc aatacccaag aagattccgg
     8821 cgcgctgccg attgaagtcg atgtatctgc tctaaaaatg ggcgatgtcg tcgatatcct
     8881 gccttatgaa ggcaaaatcg tgaaaaacgg cgagactgtt gccgagtttg aattgaaatc
     8941 acaagtattg ctggacgaag tgcaagccgg cggccgtatc aacctgatta tcggtcgagg
     9001 tctgaccgcc aaagcgcgcg aagccctgaa actgcctgcc tctactgcat tccgcctgcc
     9061 gcaagcgcct gccgaaagca aagccggttt caccttggcg caaaaaatgg tcggccgcgc
     9121 ctgcggtctg cccgaaggac aaggcgtgcg cccgggtact tactgcgaac cgcgtatgac
     9181 gacggtcggc tcgcaagaca cgaccggccc gatgacccgc gacgagttga aagacttggc
     9241 ttgtttgggc ttctccgccg atatggtgat gcagtctttc tgccacaccg ccgcctatcc
     9301 gaaacctgtc gatgtaaaaa cccataaaga actgcccgcc tttatttcca cccgtggcgg
     9361 cgtgtcactg cgtccgggcg acggcgtcat ccactcgtgg ctcaaccgcc tgctgctgcc
     9421 cgataccgtc ggcaccggcg gcgacagcca tacccgtttc cccatcggta tttccttccc
     9481 cgccggctcc ggcttggttg cctttgccgc cgcaacgggc gtaatgccgc tcgatatgcc
     9541 cgagtctgta ttggtacgct tcagcggcaa gctgcaaccg ggcgtaaccc tgcgcgattt
     9601 ggtgaacgcc atcccgctgt acgcaatcaa acaaggtttg ctgaccgttg ccaaagccgg
     9661 taagaaaaac atcttctccg gccgcatcct cgaaatcgaa ggcctgcctg atttgaaagt
     9721 ggaacaagcc tttgaattga ccgacgcatc cgccgaacgc tccgccgccg gctgtaccgt
     9781 gaagctcaac aaagagccga ttatcgagta catgaaatcc aacgtcgtgt tgatgaaaaa
     9841 catgattgcc aacggctatc aagacccgcg cactttggaa cgccgcatca aagctatgga
     9901 aaaatggctg gcaaatcccg agttgctcga agcggataaa gatgccgaat acgccgccgt
     9961 gattgaaatc aacatggacg acatcaaaga gccgattatc gcctgcccga acgacccgga
    10021 cgacgtgtgc ttcatgtccg aacgctccgg caccaaaatc gacgaagtat tcatcggttc
    10081 gtgtatgacc aacatcggcc acttccgcgc cgcctccaaa cttttggaag gcaaggcaga
    10141 cacccccgtc cgcctgtgga ttgcgccgcc gaccaaaatg gacgcgaaac aattgtccga
    10201 cgaaggacac tacggcgtac tcggacgtgc cggcgcgcgt atggaaatgc cgggttgctc
    10261 cttatgtatg ggtaatcagg cgcaagtacg cgaaggtgcg accgttatgt ccacctccac
    10321 ccgcaacttc ccgaaccgtt tgggtaaaaa cacctttgtt tacctcggtt cggcggaatt
    10381 ggcagcgatt tgctccaaac tgggtaaaat cccgaccgtt gaagaatatc aagccaatat
    10441 cggcatcatc aacgaacagg gcgataaaat ctaccgctat atgaacttca acgaaatcga
    10501 cagctacaac gaagtagccg agaccgtgaa cgtttaatcc ccgtcatccg tatgaagtaa
    10561 gggattgacc gcaatgccgt ctgaacaacc ttcagacggc attgcaacat tccgctaacc
    10621 cttctttccg caaacgctgc aaatacggcg ttcacgcccc cacataaagg aaacgacagt
    10681 gaacctgaaa aaccgccatt ttctgaaact tttagacttc acgccggaag aaatcaccgc
    10741 ctacctcgac cttgccgccg aattgaaagc cgccaaaaaa gcagggcgcg agattcagcg
    10801 gatgaaaggg aaaaacatcg ccctgatttt tgaaaaaacc tctactcgga cgcgctgcgc
    10861 gtttgaagtc gccgcgcgcg atcaaggcgc gggagtgact tatttagagc cgtccgccag
    10921 ccaaatcggg cataaggaaa gcatcaaaga caccgcccgc gtgttgggca ggatgtacga
    10981 tgccatcgaa tatcgcggtt tcggtcagga agttgttgaa gaattggcga aatacgcggg
    11041 cgtacccgtg ttcaacgggc tgaccaacga gttccatccc acacaaatgc ttgccgacgc
    11101 actgactatg cgcgaacaca gcggcaaacc tttgaaccaa accgcgtttg cctacgtcgg
    11161 cgacgcgcgt tacaacatgg gcaattccct gctgatttta ggggcaaaat tggggatgga
    11221 cgtgcgtatc ggcgcaccgc aaagcctgtg gccgtctgaa ggcattattg ccgccgcaca
    11281 cgccgccgcc aaagaaaccg gcgcaaaaat taccctgacc gaaaacgcgc atgaagccgt
    11341 gaagaatgtt gattttattc ataccgatgt gtgggtcagc atgggcgagc cgaaagaagt
    11401 ctggcaggaa cgcatcgatt tgctgaaaga ttaccgcgtt acgcccgaac tgatggcggc
    11461 atcgggcaat ccgcaagtca aattcatgca ctgcctgccc gccttccaca accgcgaaac
    11521 caaagtcggc gaatggattt acgaaacctt cgggctgaac ggtgtggaag ttacagaaga
    11581 agtattcgaa agccccgcca gcatcgtgtt cgatcaggcg gaaaaccgta tgcacacgat
    11641 taaagcggta atggtcgcgg ctttgggcga ctgacagaac tgtgcctgtt taaattcatc
    11701 cgcaacacag ataccgtctg aacacgatgt tcagacggta tcca
//
LOCUS       DAXHMX010000058        11677 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHMX010000058 DAXHMX010000000
VERSION     DAXHMX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.058"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(87..479)
                     /gene="rpsI"
                     /locus_tag="ACFM4S_001105"
     CDS             complement(87..479)
                     /gene="rpsI"
                     /locus_tag="ACFM4S_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6050802.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(489..920)
                     /gene="rplM"
                     /locus_tag="ACFM4S_001106"
     CDS             complement(489..920)
                     /gene="rplM"
                     /locus_tag="ACFM4S_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6050803.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1098..1277)
                     /gene="ssrS"
                     /locus_tag="ACFM4S_001107"
     ncRNA           complement(1098..1277)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFM4S_001107"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1283..1588)
                     /locus_tag="ACFM4S_001108"
     CDS             complement(1283..1588)
                     /locus_tag="ACFM4S_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6050804.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(1599..1928)
                     /locus_tag="ACFM4S_001109"
     CDS             complement(1599..1928)
                     /locus_tag="ACFM4S_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050805.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(1983..2972)
                     /locus_tag="ACFM4S_001110"
     CDS             complement(1983..2972)
                     /locus_tag="ACFM4S_001110"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6050806.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3095..5797)
                     /gene="ppc"
                     /locus_tag="ACFM4S_001111"
     CDS             complement(3095..5797)
                     /gene="ppc"
                     /locus_tag="ACFM4S_001111"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6050807.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPQSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            5840..6733
                     /locus_tag="ACFM4S_001112"
     CDS             5840..6733
                     /locus_tag="ACFM4S_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6050808.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            complement(7132..7356)
                     /locus_tag="ACFM4S_001113"
     CDS             complement(7132..7356)
                     /locus_tag="ACFM4S_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6050809.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(7357..8745)
                     /locus_tag="ACFM4S_001114"
     CDS             complement(7357..8745)
                     /locus_tag="ACFM4S_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6050810.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLVFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(8831..9652)
                     /gene="prmC"
                     /locus_tag="ACFM4S_001115"
     CDS             complement(8831..9652)
                     /gene="prmC"
                     /locus_tag="ACFM4S_001115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6050811.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(9642..10103)
                     /locus_tag="ACFM4S_001116"
     CDS             complement(9642..10103)
                     /locus_tag="ACFM4S_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050812.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(10168..11652)
                     /gene="tldD"
                     /locus_tag="ACFM4S_001117"
     CDS             complement(10168..11652)
                     /gene="tldD"
                     /locus_tag="ACFM4S_001117"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6050813.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRMNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDSAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLMGTQSTGNGRRESYASVPMPRMTNTFMENGGYEPDEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT         2448 a         3226 c         3266 g         2737 t
ORIGIN      
        1 atctgtaatc tttagattgc caatgggaaa ccgtctacta caaataaaaa accctgcgat
       61 aagcagggtt ttttgaattt ccaacattaa cgtttggaga attgttttgc acggcgtgct
      121 ttgcgcagac ccggtttttt acgttcgact tcgcgggcat cgcgggtaac aaaaccagct
      181 tgagacaagg cgggtttcaa cgcggcatcg aagtcgatca gggcacgggt aatgccgtgg
      241 cggattgcgc cggactggcc ggtttcgccg ccgccaacaa cattgacttt gatgtcgaaa
      301 gattcggcgt tttcagtcag aaccaagggt tggcgaacaa ccattcggct ggtttcgcgt
      361 gcgaagaatt cgtcaacggg acggccgttt acgatgattt gacctgtacc tttaatcagg
      421 aatacacgag ccactgaact tttgcggcgg cctgtgccgt agtagtattt accgttcatg
      481 tcgcgtcctt atttcagttc caaaactttg ggttgttgcg cagcatgggc gtgttccgca
      541 cccgcataca ctttcagttt tttaatcatg gcgtaaccca aaggaccttt gggcagcata
      601 ccttttacag cttgttccaa agcgcggccc gggaattgct cttgcatttc gcggaaagtg
      661 cgttcgtaga taccgcctgg aaaaccggaa tggcggaagt attttttatc ttcgaatttg
      721 gcaccggtta cacgcagttt gtccgcattg ataacaatga tgtaatcgcc ggtatcgacg
      781 tggggggtgt attcaggttt gtgtttgcca cgcagacggc tggcgacttc ggccgcaacg
      841 cgacccaaga ctttgtcttg ggcatcgatg acgaaccatt cgcgcttcac ctcgtggggt
      901 ttcgctgaaa aggttttcat agtggaaatc caaatagata tagaaagttg taaattttaa
      961 agacaggatt cgattttgtc aatcgcatta ccgcgttacg gaaggatttt ccggatttcg
     1021 gcagactgca tactgctttt tcgggcgggg cggcggccaa tgtgaaaaac cgcatcgttg
     1081 cgatgcggtt ttgaatggga atccccgcga gagccgtttc ggccgaatcc gcttgaacct
     1141 tgctgacaag gcggctgcct cgggtagttt cgggtgcgtc cgcaaaagga cgctcgcgcc
     1201 cactactgct cccggcaacc ttaagcgaac ttattggttc aaaggaatat atgccttcgc
     1261 ggacaccgca gggaaaaagg ggttattcct gcgccaagcg ggatagtgct ttttggcagg
     1321 cgttgtccat atcggctatt ttacgcgcaa aatcgccgat tgccaaatcg ccgccgttca
     1381 gggaggtttt caacaggtcg tggacgacgt tgagcgcggc cataatgacg attttttcgc
     1441 tgtccgcgac gcgtccgcct tcgcggatgg cttcggcttt gccgttgagc attccgactg
     1501 cctgcaacag tgtgtctttt tcttctgccg gcgtgttgac ggtcagccgg gcgtgcatga
     1561 cttcgatgtg gacttgttcg atgttcatcc tttaatcctt attgctgcgt ttcctgccat
     1621 tgggggaggc gcgctgccag tgcgctgatt ttttccctgc tctgttcgag caggctgcgg
     1681 tatcgtgtat tttcttctgt caggctgtca attttgtttt gcaggtcttc tttgagtttg
     1741 ccgacttgga cgagcagggc ttcgctgagt tcgtcgacgg cggtttcgtg ttcgagtttt
     1801 tgccgctcgt gcgcccgttt gagttcggcg acggtttctt tgaggcggcg gttttcgctg
     1861 acgagggttt cgaatttttg taccagcgta taaacgctgc tttcgagttt ttcgatattt
     1921 tgtttcataa ccttacctgt ccgtatgccg tctgaaggcc tcagacggca tctgtctgtt
     1981 gtttattcga aacgcgcgct gcgttccatc agtctttcga cgacctgctg aggggtcatt
     2041 tccttacgga tgagttgcag cagggtttgg gtaatcggca tatcgatttg gtacttacag
     2101 gcagtattga agacttcttc tatcgtgctg accccttcgg aaacgtgtcc gatttcgacc
     2161 agcacctgat gcagttcctt gccttctgcc aaacccaagc cgacgcggcg gttgcgtgag
     2221 agtgcgccag tgcaggtcaa gatgaggtcg ccgatgcctg ccagccccat catggttttg
     2281 ggctgtgcgc ccattgcgga ggcaaggcgg gtaatttcag ctaatccgcg cgtaaccagt
     2341 gcggcacggg cgttaagccc gtactctagg ccatcggaca atccggtggc aatcgccata
     2401 acatttttta ccgcgccgcc aaccgccacg ccgataacgt cggtactgcc gtaaagcctc
     2461 atgacagtcg tgttgagctg cggtacgagt tcttcaatcc actcttggtt ttcggaggcg
     2521 aggacgacgg cacagggcag ttgtttggcg agttcctgtg caaaactcgg gccggagagt
     2581 acgccgattt tcttattgtc gggcaatact tctttcaata cttgaaaggt cagcagccct
     2641 gtatcctgct cgaatccttt gcaggcggcg aggacgggga ggtgtcccgc gccgtactgt
     2701 ttgagcagct ctgcgctgct tctcaatccg gcaacggagg ttacgataag gacaagtccg
     2761 ctgtctttga gcgcgtctgc caaatccgca cacacttcca aggtttcggg aaaggaaaag
     2821 ccgggcagtc cgcgtttgtt ttcacgcgct tcctgcattt ggcggacttg gtctgcgttg
     2881 cgcgtccaca gggatacgcg gttgccgtgt tgggaaaagt gcagggcgag cgccgtaccc
     2941 cacgaacctg cgccgataac ggtaattttc attggtcgtc tttcaacata tcactgccgt
     3001 tcactttaaa acaatcggtg tttctctgca agtgcggtca gggaaatgcc gtctgaaagg
     3061 tgttcagacg gcattttgcc ccgatgcggc acaatcagcc tgtattgcgc aaaccttgcg
     3121 ccacgccgtt gatggtcagg tgaaccatca gcagcgcgtg cggattgtcg ggttctttac
     3181 gcaggcgttt gagcatggcg acctgcaggc cgttgagcgc gttcaggtag ggaatcctca
     3241 aggcaagcga acgggcgagg ctgcggttgt cgcgtaaaag ttcttcggtt tgcagcaggt
     3301 cgagcagggc tttgcggctg cgttggtatt cttccttaat catgccgaag atgacggcgg
     3361 ctttttccgg cgattcgctc aagccggcat agttttccgc gagggtgatg tcggttttcg
     3421 ccatcacttg ctccatattg gaaagcatgg cttggaagaa cgggttgctt tgggcgtgtt
     3481 cgcgcagggc ggcgagcgtt tcgggtttgt cttcgcacaa ggtttccacc gcgctgccga
     3541 aaccgtacca agcaggcagc atgaggcggt tttgcatcca ggaaaatacc cacggaatcg
     3601 cgcgcaagtc ttgaatccgc gctaaggtct tgcggctggc aggacggctg ccgaggttga
     3661 gggtggcgat ttcctgaatc gggctggttt gcaggaagta gtcgatgaag tcggggtggg
     3721 taatcagttc gcggtagtat ttgaacgata cgtccgacaa tgcctgcatc agtttggcat
     3781 cagggtcttt tttatccggc aggatgctgg cttccaaagt cgcggcaacc aaggtttcca
     3841 agttgcgttg ggcattgccg gggtcggcgt atttggcggt aatgacttcg ccttgttcgg
     3901 taatgcggat ttgtcccgcc acgctgccgg ccggttgcgc caaaatggct tggtaagaag
     3961 ggccgccgcc gcgtccgacg ctgccgccgc gtccgtggaa caggcgcatg cggacatcgt
     4021 attttttgaa gagttcgacc aagcccaatt ccgcctgata gaggcaccat gagctggtaa
     4081 cgtagccgcc gtccttgttg gagtcggagt agcccagcat gatttcttgg atattgccgc
     4141 ggctttcgag cagggcatcg taccagtcga ggcggaacat ggtttccatg accggacagg
     4201 cgttttccaa cgcttcgatg gtttcaaaca gcggcacgat attgatgcgg ctttgcggtt
     4261 tgccgttttc taccgccaac aggccgcttt ctttcagcag caatgccaag gcgagcaggt
     4321 cgctgggttg ttcgcagttg gaaataatgc tttgcgtaac ggcatcttcg ccgaattcgt
     4381 ctttgatttt gcgcgcttca ttgaaaatcg ccagttcgtg gcgagtatgg tcgctgtatg
     4441 tgatgaacgg actgtacagt ggacgttgat ggctcaattc gcgcaacagg gcggcttgtt
     4501 tttgctcttc attcaggctg ttgtagtctt ccaagcctgc gtgttggaaa agctcggcaa
     4561 ccacatcggc gtgtttgcct gcgtgttggc gcaggtcgag cggcatcatg tggaagccga
     4621 acacggacac ggaacggatg aggtctgcca gacggccttc ggcaagcagg cggctgccgt
     4681 tgtcgataag ggaacgttgc aattttttca aatcatcgag aaattcttgt gccgaagcat
     4741 aaggctcgag aaagccgaat ttgcagccca tgcccaaacc gagcgcgcgc gctttgccca
     4801 tagcgcgcgc catgatgtaa gcaatggcgc ggcggtaggg ttcttccgtg cgggcaagtt
     4861 cttcgtcagg cgagagggct gccagtgcca ttacgtcgtc gttgactttg acgcggcgga
     4921 tggagagcgg cagttcgcgg taaagtttgt cgagttcgcc acggtaaaaa cggaacacgg
     4981 catcggcgtg gcggcggaag gcaaaacgca aggtttcggc agaaacgaac ggattgccgt
     5041 cgcggtcgcc accgatccag ccgccgattt tgaggatgtt cggaacgcag acatcgggat
     5101 agaccatctg aaagtcgtgt tccatattgc ggtagagctt gggcagggct tcgaaaaagc
     5161 tcatcgggaa gatggacacg ccgttgttga tttcgtcgtt gacgctgagt ttgtggcggc
     5221 gcgtttcgct ggtctgccac aagcccagca ggatagtgtc gatttcgcgg cgcagccgtg
     5281 ccagcgcgtc ggcattggtg cagcgttcgc gttgcggcaa cagtgcgcgg atgcggcggt
     5341 tgaagcttaa gacggtttgg cgttgcactt cggtcgggtg cgcggtcaaa acggcggtaa
     5401 cggacgtatt gtccaactgc cgctgcaccg atttgccgtc ggctttcccc gctttgagcc
     5461 tgcggacggt ttccgtcagg ctgccttccg cgccgccgcg tccggcttct tcgtggattt
     5521 ggcggcggcg ttcgtggtgc acgtcttcgg cgatattcaa aatctgggcg aacaggccgc
     5581 aggccaaggt taaatcgtgg gtttgttgtt cgtccaattg cggcaatact ttttcaatca
     5641 atgccgcgct gtcgtcggaa gtggacaaga gtttgaccgt ttcgacaacc aatggcgagg
     5701 cttcttcgtg caggaggttg aacagggatt gtttcagaaa ttccgcgtcc gccgccaaag
     5761 ccgcgtcctt tggattgttc agaatatgca gttgcatgat ttttctctca ttgccgtaaa
     5821 tactgtaaat gtacctcaaa tgccgcatcc gtgccaaacc gttcacactt taaccgcccg
     5881 tgtcccgaaa tgccgtctga agttgaacgc cgcccgacgg cagcgttaca atcgcccaca
     5941 actgtttttt ccgaacatca tcatgaccac gaccgaacac gacaacgacg atgcattcct
     6001 gctgcggtac agccgccaca tcctcttgga cgaaatcggc atcgaagggc agcagaaact
     6061 ttccgccgcg catattttgg tcgtcggctg cggcggtttg ggtgccgccg ccctgcccta
     6121 tctcgccgct tccggcatcg gcacgctgac catagccgat tccgacacgg tcgagctgca
     6181 caacctgcaa cgccaagtcg catttgacga gggcgatgtc ggcaagccca aagccgaagc
     6241 cttggcagac cgcctgaaac gcatcaacca taacgtcgat gtccgcaccg tcaacgaaaa
     6301 actcgacggc tgccgcctga ccggtttggt tcaaaccgcc gacatcgttt tagactgctg
     6361 cgacaactac gccacgcgcc aagccgtcaa ccgtgtctgc gtacaaacga aaacaccgct
     6421 ggtttcagga gcagcggtgc gctttgaagg gcaacttgcc gtgtaccgtc ccgacttgcc
     6481 cgactcgccg tgttacgcct gcctgtttga cggcggatcg gcttcagacg gcatctgttc
     6541 cctcttcggc gtgttctcgc cgctggtcgg catcatcggc agtacccaag cggcggaggc
     6601 tctgaaaatc ctgctggatg cgggcgaacc gtcgcacggc aggctggcgg tttaccgtgc
     6661 cttggaaggg ggctggcaat atttcgacct gccgcgcaac cccgaatgcc cgatttgcgg
     6721 cgcggaacgg taaaaccctg ccgccgtttc agacggtatc caaacggatg cggcggaacg
     6781 gttttaaaaa tttaaaaatt tacatttctt tgcaaaaaaa ataaacttac cttataattg
     6841 cagttgtttt agcaatgtct gtttcgcaga ctcattgagt aagacgtttt ccccgtaatg
     6901 tgtttggccg tctgtcccct ttgggttcgg acggcttttt tttggctgtg tatgaatacc
     6961 cggttggttt tatctgttta caggggaaaa ccgcttattt ccgttcgggc ggaaaacggt
     7021 tccatcggat aaaaggcatt ttgtccgact gattaaagtt acatcttagg tttgcatcgg
     7081 caaaatattc aaacacagcc ttttttaagg aaatccggat acggcggcgc atcaataatg
     7141 cggcggaatc tcgtcgcgca gggaatacgg ctcttgcgcg tcgggattcc tgtcctgcat
     7201 tttttgatac agcagcctca actgagcctg ctgcaaatcc agcgtctgcc gcaattccgc
     7261 caccatcgcg ttcaggccgg cgattacgtc ctcctgaagc gcggattgga tttccagttc
     7321 gacaatacgg cgttccaact cttgaaccgc gtccatttac agcaccatcg cggcaatcca
     7381 gccggcaatc agcagcggga tgttgtagtg gatgaaggtc gggataacgg aatcgcggat
     7441 gtggtcgtgc tgcccgtcgg cgttcagccc catcgtcggg cccagcgtgg aatcggacgc
     7501 aggcgaaccg gcatcgccca acgcccccgc cgtgccgaca atggcgacgg tggcaagcgg
     7561 cgaaaaaccc aaaccgacac acaaaggcac ataaatcgcg gcaataatcg gcaaagtgga
     7621 aaaggacgaa ccgatgccca tcgttaccaa aagccccacc accagcatcg ccaacgccgc
     7681 catacctttg ctgttgccga atatcgccat actgctttcc accagcggct gaatatgccc
     7741 ggtcgcattc atcacggcgg caaaaccctg cgcggcaatc ataatgaagc cgaccatcgc
     7801 catcatcttg atgccttcgc cgaatacgtc gtttgccttg tcgcggttga tgacgcccaa
     7861 catcataaat acggcgaaac cgagcatcgc gcccaacacc agcgagtctt catacatcaa
     7921 ctggatggca aagcatacgg caatggcggc ggcggcggcc aggctgcggt aggcggacgg
     7981 ctgcggacgg tttgccgcat cggcgttgcc cgccgtatcg gcattgttgc tttggtacag
     8041 gcgcggtttg cggtaatgga caaacaccag caggagtccg gccagcattc ccaacgcggg
     8101 aatcgccatt gccgccatta cgttgatgtt tttcacatca agctgcggcg cggcggaatg
     8161 gatgttgccc aacaggattt cattcaaaaa aatcgcgccg aagccgtaag gcaggaacat
     8221 ataagtcgta accagcccga aagtgatgac gcacgcaatc aggcggcggt cgattttcag
     8281 gcggttgaac accaaaagca gcggcggaac aatcatcggg ataaaggcga tgtggatggg
     8341 gatgatgttc tgactcataa tgcccatcac aaggatgatg gaaagcagca gccatttgac
     8401 cgcgccctcg cccgaacgca cgctgtcggg cataccgccc cggttcagct tgcggacgac
     8461 cgcgccggca agctgttgcg gcaggccgga atgggtaatc gccattgcaa acgcgccgag
     8521 catcgcataa gaaagcgcaa tcttcgcacc gccttccaaa cctttgttga acacggggat
     8581 aatccccgcc tgactgacct gtcccgccgc atcggcaatg ttttgcagcg gcatacccgc
     8641 caccgcgccg ccgacaaacg cgccgaccgt caggctcaat accacgtgca cgcgcgacag
     8701 cgacagcacc agcataacga ttacggcaac tacgactgca ttcattgttt atcgctccaa
     8761 acctataaat gtttacatat cgaaacacat cataacccaa taatggaaaa cccgccaatt
     8821 ttgcaaacaa ttatttcaaa tgcttcatat acttccccag cgtaaccctg tccaaacccg
     8881 ccaagtccgg cagggtttcc actcctgaaa aaccattctc cgccaacacg ccgcgcaccg
     8941 ccgcgccctg atcgaaaccg tgttccaaca acaaaaaacc gccttccgcc aaacggtcgg
     9001 gcgcgccttg cgccaaggtg cggatgcagc ttaggccgtc tgaaaagtcg gtcagcgcga
     9061 tttgcggctc aaaccgcaaa tcgccttgca acaaatgttt atcgccgttt tcgatatagg
     9121 gcgggttgga cacgatgatg tcccatttcc cttcagacgg catatcggtg tcgaaccacg
     9181 aaccgtgtgc aaattcgacc cgcgcgccca aatctgccgc atttttccgc gccgtttcaa
     9241 gggcgggcgt gctgatgtcg gatgcgcgca caaacgcatc ggggcgttcg agtgcgacgg
     9301 ttacggcaac cgcgccgctg cccgtcccca aatcccacac gcgcccgttt tcgggcaggc
     9361 gcgccaatac ggcttcgacc aagtgttcgg tttcggggcg cggaatcagc acgctcggat
     9421 tgactgtaaa gcgtctgcca taaaattcgc gcacacctaa aatataggca accggctcgc
     9481 cgttcagacg gcgttgcgcc agcctgtccg cccgctgtcg gacttcgtcc ggcatttctt
     9541 ccccgccccg cgtcaacaac tgcacgcgcg tatattccga aacatattgc agcagcattc
     9601 ttgcttcaat tttaggcagt tttgacaagc ccaaccattc gtcaaacgtc attttttatc
     9661 ccgtctgccg ccgatgcggc ttttctttcc ttattctttc cggcaaacgt accgatggtg
     9721 gcaaccgcaa atgcggcata ccacaaataa aatcctgcac cgtagcgcac aatatccgat
     9781 gtattccctg cttcatcgac gtatacggct ttcacactga aagccaccaa cgccaagccc
     9841 caaagtgccg catggacagg cacgaccttc ttccgcaacg ccagcaaaac aatggccgcc
     9901 aaccaaacat aattcgcata gaccgcacaa tacctgatat ccaaagaagc aaatatcgac
     9961 cccaaaatca aaacggtcaa accctccatg cttccatgat tgcccaaata aaatgcaaca
    10021 ttggataaag acgctatcca cagggcaacc gacaccagca acatcactat gggaaaactt
    10081 gatttccgat tctgttcctg catggtttta tcctaatgta aaaggccgcc tgaaaacctt
    10141 tcagacggca tcgtgccgga ttccgcgtca gattgcgctg ccgccgacgg tcagtccggc
    10201 atcaatccgc aaagtcggtt gccccacgcc gacggggacg ctctgccctt ctttgccgca
    10261 cacgccgaca ccgctgtcga gcgcagtatc gttgcctatc atggaaacgt gtttcagcac
    10321 ttcggggccg ttgccgataa tggtcgcgcc tttgacgggg tattgcagcc tgccgccctc
    10381 cacccaccac gcttcggacg cgctgaacac gaatttgccg ctggtaatgt ccacctgtcc
    10441 gccgccgaag ttgacggcgt aaatgccttt gtcgatggac gcgatgattt cgtcgggttc
    10501 gtagccgccg ttttccataa aggtattggt catgcgcggc atagggacgg aggcgtagct
    10561 ttcgcggcgg ccgttgccgg tcgattgcgt gcccatcagg cgggcattgg tttcgtcctg
    10621 catataaccg accaagatgc cgtcttcaat caatacggtg cggcgggttt cgttgccttc
    10681 gtcgtcgatg ttgagcgaac cgcgccggcc ggcaatatcg ccttgatcca caacggtaac
    10741 gcctttggcg gcgacgcgct cgccgattcg gccggaaaag acgctggttc ccttgcggtt
    10801 gaaatcgcct tccaaaccgt gtccgaccgc ttcgtgcagc aacacgcccg gccagccgtt
    10861 gcccaaaacg acggtcattt cgccggcagg cgcaggacgg gattcaagat tggtcagggc
    10921 ttgtttgacg gcggaatcga caaaccgatg caccaagttt tcatcgaaat aagccaagtc
    10981 gtagcgtccg ccgccgcccg cgctgccctg ttcgcggcgt tcgccctgtt tggcgataac
    11041 ggtaacgttc atgcgcacca tcgggcggat atcggcagcg tgtttaccgt ccagacgggc
    11101 gaggtaaacc atgtcgtatt cgcaggtcaa accggccatc acttgcacga tgcgcggatc
    11161 ggcggctttg gcgattgctt ccactttgtt caacagcgcg actttggcgg cggaatcgag
    11221 gccggcaatg gggtcggacg cggaacaaac cggcttgccg cgcgtttcag acggcatttt
    11281 ggcggacacc ttgccgcctg ccgccccaat cgcgcggacg gcgcgggcgg aacggtttat
    11341 cgaatcgatg cacaggctgt cggcgtaggc aaaggcggtt ttatcgcccg aaacggcgcg
    11401 cacgcccacg ccctgattga tttggaagct gcccgatttg acgatgccct cttccaaatg
    11461 ccagctttca taagcggtgc gctggcagta gatgtcggcg taatcgacgt ggtgcgcgcc
    11521 gatgatgcac aggcttttgg cgagcagttc gggggaaagg cggttggctt cgagcagccg
    11581 cgcctgtacg gcggaatagg tcggatgcat agtgtcggcg cataaaaaat caggggcttg
    11641 attatacggc atttgttata tagtggatta acaaaaa
//
LOCUS       DAXHMX010000059        11416 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHMX010000059 DAXHMX010000000
VERSION     DAXHMX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.059"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..79)
                     /locus_tag="ACFM4S_001118"
     CDS             complement(<1..79)
                     /locus_tag="ACFM4S_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6050814.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="ACFM4S_001119"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="ACFM4S_001119"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6050815.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKTVSI"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="ACFM4S_001120"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="ACFM4S_001120"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6050816.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="ACFM4S_001121"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="ACFM4S_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6050817.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSGHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLVGKSVEALKNYLSLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /gene="fba"
                     /locus_tag="ACFM4S_001122"
     CDS             4804..5868
                     /gene="fba"
                     /locus_tag="ACFM4S_001122"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6050818.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5888..6850
                     /locus_tag="ACFM4S_001123"
     CDS             5888..6850
                     /locus_tag="ACFM4S_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6050819.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYISSILRMTRS
                     KPVNRTAFCCFSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQVYKQSHSALTALQTEQEQDPEHSGKMVAKRRFKIGDIAGEHTSFDKLPKDVMATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVELATAYIKPDEKHHAVISGS
                     VLYNQDEKGSYSLGIFGGQAQEVAGSAEVETANGIHHIGLAAKQ"
     gene            6989..7666
                     /gene="tsaB"
                     /locus_tag="ACFM4S_001124"
     CDS             6989..7666
                     /gene="tsaB"
                     /locus_tag="ACFM4S_001124"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6050820.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETCLFHQEVGSRQSALI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGVPSLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHSELLYVRNKIALTAKEQ
                     AERKART"
     gene            7663..8103
                     /gene="rimI"
                     /locus_tag="ACFM4S_001125"
     CDS             7663..8103
                     /gene="rimI"
                     /locus_tag="ACFM4S_001125"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6050821.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8097..8834
                     /locus_tag="ACFM4S_001126"
     CDS             8097..8834
                     /locus_tag="ACFM4S_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6050822.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8896..9537
                     /gene="pyrE"
                     /locus_tag="ACFM4S_001127"
     CDS             8896..9537
                     /gene="pyrE"
                     /locus_tag="ACFM4S_001127"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6050823.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9613..10047
                     /locus_tag="ACFM4S_001128"
     CDS             9613..10047
                     /locus_tag="ACFM4S_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6050824.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10044..11354
                     /gene="argA"
                     /locus_tag="ACFM4S_001129"
     CDS             10044..11354
                     /gene="argA"
                     /locus_tag="ACFM4S_001129"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6050825.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKAYRSNGRNSHILVRRLHR"
BASE COUNT         2560 a         3612 c         2977 g         2267 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatac agccgtcctg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgttga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctcattc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtt aaagaaatca acctcttagg ccaaaacgtc aatgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgacttta
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaacgat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgcccg atatgatcaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaaaccgt cagcatttga tttcccccgc
     1621 cgcaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatgctg
     2041 ccaaaggcaa tgaaggcagt tttcccacct tcgcggcgga taatgccctt gccgatttca
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg cagcgcaaga agctcaaatc gtacaaaccg
     2281 gcgtgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg cgatgccgac atcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgctt tctttaggca ggttggcgac ggcgtggtat
     2701 ttgacgggat cgttttcggc gagtttgtag ccgttgcctt ttttggtaat gacgtgcagc
     2761 aactgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatacccaa agccgcgccg
     3241 atggaagtgg acgaatgccc cacgccgaac gcgtcgtatt ccgactcaca acgtttcgga
     3301 aaacccgcca agccgccgta ttggcgcatc gtgtgcattt ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgtcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca gattgctggc gaaatgaccg
     3481 ccggtctgcc cgacagattc cagcagaaag gcgcgcagct cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggtcaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gtttggtgta aatctgcgtg
     3781 gtcgaaaggc tgctgtgtcc gagcagctcc tgcaccgccc ggatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcga cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagc tcgaacaggg cgtgatcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt tgaccagcca ggcgcaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgttgc
     4441 gacaagccct gcggcacgcc gcctgcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgcc ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggct gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc
     5221 gaaggcgaaa tcggcgtgtt gggcaacctc gaaactggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat tcacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gttccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgataccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatatttgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgt
     5761 tatcttgcgt tcggctgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaaacc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgcttgt
     5881 aaacaaaatg ccgtctgaac cgttgttcgg acggcatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata tatcatctat
     6001 tctgcgtatg actaggagca aacctgtgaa ccgaactgcc ttctgctgct tttctctgac
     6061 cgccgccctg attctgaccg cctgcagcag cggaggcggc ggtgtcgccg ccgacatcgg
     6121 cgcggggctt gccgatgcac taaccgcacc gctcgaccat aaagacaaaa gtttgcagtc
     6181 tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc
     6241 ggaaaaaact tatggaaacg gcgacagcct taatacgggc aaattgaaga acgacaaggt
     6301 cagccgtttc gactttatcc gtcaaatcga agtggacggg cagctcatta ccttggagag
     6361 cggagagttc caagtgtaca aacaaagcca ttccgcctta accgcccttc agaccgagca
     6421 agaacaagat ccagagcatt ccgggaagat ggttgcgaaa cgccggttca aaatcggcga
     6481 catagcgggc gaacatacat cttttgacaa gcttcccaaa gacgtcatgg cgacatatcg
     6541 cgggacggcg ttcggttcag acgatgccgg cggaaaactg acctatacta tagattttgc
     6601 tgccaaacag ggacacggca aaatcgaaca tttgaaatcg cccgaactca atgtcgagct
     6661 tgccaccgcc tatatcaagc cggatgaaaa acaccatgcc gtcatcagcg gttccgtcct
     6721 ttacaatcaa gacgagaaag gcagttactc cctcggtatc tttggcgggc aagcccagga
     6781 agttgccggc agcgcggaag tggaaaccgc aaacggcata caccatatcg gtcttgccgc
     6841 caagcaataa ccattgtgaa aatgccgtcc gaacacgata atttaccgtt cggacggcat
     6901 ttttgcgcca taccgcccta ggacacgacg gcacggcagg caagccggtt tcactatata
     6961 atgccgtctg aaccaacgag agaatgccat gcaagctgat tttaaccgcc ccgtcctcgc
     7021 cgtcgatacc ggaacttcct atttgtcgct cgcgctgcgc gccgacggcg aaacctgcct
     7081 gttccatcag gaagtcggca gccgccagtc cgcactgatt ctgccggaaa tccgcacgtt
     7141 gtttcacgat gcaggcatta ccgccgccga tttgggggcg attgtttatg cgaaaggccc
     7201 cggcgcgttt acgggactgc gtatcggcat cggcgtggcg caaggtttgg caacgccgtt
     7261 tgatactcct ttaatcggcg taccctcgct cgatgccgcc gcctcgctgc cgccgccgca
     7321 aagctgcata ctcgccgctg cggacgcgcg tatgggcgaa gtgttttatg cgtggttcga
     7381 cacgctgaac cgccgccgtt tgagcgatta tcaggtcggg cgggcggcag acatccggct
     7441 gccggaggga tgtgcctttt cagacggcat aggcagcgcg ttcgcgttgg aagaaacgcc
     7501 gccgttttca ggcaaacccg atatgccgac cgccgccgac ttcctcaatc tggcggcgga
     7561 aggcggtttt cccgccgtcg ctgctgcaca ctccgaactg ctctacgtcc gcaacaaaat
     7621 cgccctaacc gccaaagaac aggcggaacg gaaagcccga acatgaacat ccgccgcgcc
     7681 tcaccgtccg actgcgccgc actcgccgcg ctcgatgccg tctgcaaccc gtccgcgtgg
     7741 acgcaacgcc aatttgagtc cgcattggtt tcgccgtccg aacaggtttt cctcgcggaa
     7801 aaagacggcg ggattgccgc ctttatcgtt tggcagaacc tgcccgacga atccgaactg
     7861 cacctgattg ccaccgcgcc cgaatgccgc cgccgtggcg ttgcgtccgc cctgctcgaa
     7921 tattggttcg cacatctgcc cgaagacacg caacgcctgc tgctcgaagt ccgtgcaggc
     7981 aacaccgccg cacaggcact gtacgcggcg cacggcttta ccgatgcggg caggcggaaa
     8041 aactattacc gtacagccga cggtaaaacc gaagatgctg tcttaatgga gaaaatatgt
     8101 taagcgcgcg ctacctccac ctgcacgaag ctttgggttt aggcccgatg tggctgaaac
     8161 aggccgccgc cgtcctgccg cccaaaaaca cacccgcaac cccggcacag gcacgtcctc
     8221 aaaccgtccg cgccgccccg atccgccctt cccaacccca taacggtcag gcgcggctcg
     8281 aaacgatgaa agcgttggaa accgccgccg tacatacgcg caaacccgcg cctgaaaccg
     8341 aaacgccccc gcccggcctt tcagacggca tcgcccccgt tcccgccgct tcgggcataa
     8401 ccaaacttgc cgtcgtcagc ctttgcccac cgattgagga tgcggtttac gggcaactgt
     8461 tctacggcaa agcaggtgtt ctgcttgaca acatactcaa agccgtagga ctggatgccg
     8521 cctatgtcca caaaacctgt tgggtgaaaa ccgccgccgt cggcaacccg atgccgtctg
     8581 aagcggcaat cgaaaccgcg acggtttccg tcatgcagga actcgacggc tgccgcgccc
     8641 ccgccgtcct tttcctcggg caggcttttg tccagcctga acggcaaacg atgattgaaa
     8701 ctttgtgcgg cagccgtccc ttcttcatca tcgaccatcc cgcccggctt ttacgccaac
     8761 ccgaactcaa agcccgcgcc tggcaggtgt tgaaacagtt gaaacgcgcc ttgcggcaag
     8821 gcggcggcag ttgaagcgcg ccgcacgggg cggtagaatc gcaactgcgt cccaatatct
     8881 gacagaaagc acaaaatgac cgatttccgc caagatttcc ttaaattctc cctcgcccaa
     8941 aatgttttga aattcggcga attcaccacc aaagccggac ggcggtcgcc ctatttcttc
     9001 aatgccggtc tctttaacga cggcttgtcc acgctgcaac tggcaaaatt ctatgcacaa
     9061 tccatcattg aaagcggcat ccgattcgat atgctgttcg gtcccgccta caaaggtatt
     9121 attttggcgg cggcaaccgc gatgatgctg gcggaaaaag gcgtaaacgt cccgtttgcc
     9181 tacaaccgca aagaagccaa agaccacggc gaaggcggcg tgttggtcgg tgcgccgctt
     9241 aaagggcgcg tgctgattat cgacgacgtg atttctgccg gcacatccgt acgcgaatcg
     9301 atcaagctga ttgaagcgga gggcgcaacc cccgccggcg tcgccatcgc gctcgaccgc
     9361 atggaaaaag gcaccggcga attgagcgcg gttcaggaag tggaaaaaca atacggtctg
     9421 cccgtcgccc ccatcgccag cctgaacgat ttgttcatcc tgttgcaaaa caaccccgaa
     9481 ttcggacagt tcctcgaacc cgtccgagcc taccgtcggc agtacggcgt agaataaaaa
     9541 caaagcatat gccgtctgaa ccgccttacg cctcagacgg catcaaaccc ctgacacaca
     9601 cgaggaaata ccatgcccgc ctgtttctgc ccccactgca aaacccgtct ctgggtcaaa
     9661 gaaacccaac tcaatgtcgc ccaaggcttc gtcgtctgcc aaaaatgcga aggactgttt
     9721 aaagccaaag accatctggc aagcacgaaa gaacccatat tcaacgattt gcccgaggct
     9781 gtttcggatg tcaaactcgt tcaccgtatc ggcacgcgcg ccatcggcaa gaaacagatt
     9841 tcccgtgacg aaatcgccgg catcctcaac ggcggcacaa cccagcccga tattccgccc
     9901 gcaaccgccg ccacccctgc tgccgcaccg caggttaccg taccgcccgc cgcgcccgcc
     9961 cgtcaggatg ggttcaactg gacgattgca accctgtttg cccttatcgt cctcattatg
    10021 cagctttcct acctcgtcat cctatgatcg tgcccgacct ctttgtcgcc cacttccgcg
    10081 aagccgtccc ctacatccgc caaatgcgcg gcaaaacgct ggtcgccggc atagacgacc
    10141 gcctgctcga aggtgatacc ttaaacaagc tcgccgccga catcgggctg ttgtcgcaac
    10201 tgggcatcag gctcgtcctc atccacggcg cgcgccactt cctcgaccgc cacgccgccg
    10261 cgcaaggccg cacgccgcat tattgccggg gcttgcgcgt taccgacgaa acctcgctcg
    10321 aacaggcgca gcagtttgcc ggcaccgtcc gcagccgttt tgaagccgca ttgtgcggca
    10381 gcgtttccgg gttcgcgcgc gcgccttccg tcccgctcgt atcgggcaac ttcctgaccg
    10441 cccgtccgat aggtgtgatt gacggaaccg atatggaata cgcgggcgtt atccgcaaaa
    10501 ccgacaccgc cgccctccgt ttccaactcg acgcgggcaa tatcgtctgg ctgccgccgc
    10561 tcggacattc ctacagcggc aagaccttct atctcgatat gcttcaaacc gccgcctccg
    10621 ccgccgtctc gcttcaggcc gaaaaactcg tttacctgac cctttcagac ggcatttccc
    10681 gccccgacgg cacgctcgcc gaaaccctct cggcacagga agcgcaatcg ctggcggaac
    10741 acgccggcgg cgaaacgcga cggctgattt cgtccgccgt tgccgcgctc gaaggcggcg
    10801 tgcatcgcgt ccaaatcctc aacggagccg ccgacggcag cctgctgcaa gaactcttca
    10861 cccgcaacgg catcggcacg tccattgcca aagaagcctt cgtctccatc cggcaggcgc
    10921 acagcggcga catcccgcac attgccgccc tcatccgccc gctggaagaa cagggcatcc
    10981 tgctgcaccg cagccgcgaa tacctcgaaa accacatttc cgaattttcc atcctcgaac
    11041 acgacggcaa cctgtacggt tgcgccgccc tgaaaacctt tgccgaagcc gattgcggcg
    11101 aaatcgcctg ccttgccgtc tcgccgcagg cacaggacgg cggctacggc gaacgcctgc
    11161 ttgcccacat tatcgataag gcgcgcagta tgggcataag caggctgttc gccctctcca
    11221 caaataccgg cgaatggttt gccgaacgcg gctttcagac ggcatcggaa gacgagttgc
    11281 ccgaaacgcg gcgcaaagcc taccgcagca acggacggaa ctcgcatatt ctggtacgtc
    11341 gcctgcaccg ctgaccgcaa cggaaagccg ccgcagaaat gccgtctgaa ccccgtttca
    11401 gacggcattt ccccga
//
LOCUS       DAXHMX010000060        11179 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHMX010000060 DAXHMX010000000
VERSION     DAXHMX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.060"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(192..404)
                     /locus_tag="ACFM4S_001130"
     CDS             complement(192..404)
                     /locus_tag="ACFM4S_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6050826.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(416..697)
                     /locus_tag="ACFM4S_001131"
     CDS             complement(416..697)
                     /locus_tag="ACFM4S_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050827.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(1026..1598)
                     /locus_tag="ACFM4S_001132"
     CDS             complement(1026..1598)
                     /locus_tag="ACFM4S_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC6050828.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            complement(1573..1917)
                     /locus_tag="ACFM4S_001133"
                     /pseudo
     CDS             complement(1573..1917)
                     /locus_tag="ACFM4S_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1910..2785)
                     /locus_tag="ACFM4S_001134"
     CDS             complement(1910..2785)
                     /locus_tag="ACFM4S_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFC6050829.1"
                     /translation="MSNEVEVMDAVSVQNYQVAHSVMVMEQWGNGEVYNEATWIERGR
                     LAVRQTMEGMFELGRALIVLKEHTEHGRFMEIVKGQFGIGHNETARLMSATQRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2831..3121)
                     /locus_tag="ACFM4S_001135"
     CDS             complement(2831..3121)
                     /locus_tag="ACFM4S_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6050830.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRRQHERLQSKIAEIGKRVNAD"
     gene            complement(3133..3378)
                     /locus_tag="ACFM4S_001136"
     CDS             complement(3133..3378)
                     /locus_tag="ACFM4S_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6050831.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAQNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGYEIAKKLGLK"
     gene            complement(3371..3595)
                     /locus_tag="ACFM4S_001137"
     CDS             complement(3371..3595)
                     /locus_tag="ACFM4S_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6050832.1"
                     /translation="MEKPLNFKPIPYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            3756..4238
                     /locus_tag="ACFM4S_001138"
     CDS             3756..4238
                     /locus_tag="ACFM4S_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6050833.1"
                     /translation="MSFQSVHFFVLSRDDLGCLCFDVEIIGRILPILQGIFMNNSSFF
                     GNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGISQPKAELFFQFEKVGIDVQYVM
                     HGRRGETAVMPSETLNAEEQELLALFREAAAADREMILMVARRAEKKAQTALGKVSNG
                     "
     gene            4231..4863
                     /locus_tag="ACFM4S_001139"
     CDS             4231..4863
                     /locus_tag="ACFM4S_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050834.1"
                     /translation="MDKMNQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLW
                     ARSAKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSI
                     YDLLGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKK
                     FVMPCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            4856..5050
                     /locus_tag="ACFM4S_001140"
     CDS             4856..5050
                     /locus_tag="ACFM4S_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6050835.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5074..5634
                     /locus_tag="ACFM4S_001141"
     CDS             5074..5634
                     /locus_tag="ACFM4S_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050836.1"
                     /translation="MKILALLIAATCALSACGGQAEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSVANKGLADQKTGLTLPEHVVPADNAEGKNLLHDFSDGLTLTVDTDKADKITA
                     VRVVWNTDAMPQKAEKLSKAAAALIAATAPGDRTLLRDTGDQIKMAIDSHNAQKEPTR
                     EWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            5775..6833
                     /locus_tag="ACFM4S_001142"
     CDS             5775..6833
                     /locus_tag="ACFM4S_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050837.1"
                     /translation="MKNKLTLRYFKITRCGYWSGSSKEPNHYEDLLKTLTHLETYVKG
                     KTIEETGISRDDKEPSTFLLDICKKNGLWLLAFWNPAAESSSDEIVSLNMKSKLGQVD
                     SSPVTAGNGKTFGFVSYFLIWPEHNFYSTILSPNATLAGRTEFEEYMLNFLWIDPNFI
                     EKTTYEETSTDGIIHTMERTNFKLPLKINGTPVPRFAGKLAKSPSEKEYVISNYDQVT
                     QVHNQCTVKVTSGQSSSGILKKMLPSFFGSTELFAPLKTIRVNHSVSAAFENKSNLKD
                     WIEDWEKNSFDLDKDDCGFKLKNDDKIYWIRGNVIKCELDVNLHKENGIYPAAKILDI
                     IDQRKDYVLNEFKLSDDS"
     gene            6823..7329
                     /locus_tag="ACFM4S_001143"
     CDS             6823..7329
                     /locus_tag="ACFM4S_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050838.1"
                     /translation="MILRNIFLICSAFMIGVAWYLGANIRFEEQWVLYEALRTTASII
                     FAVVGVWLAIVYPERLKSPFSQTFSSPVYRRGFKQLFSPVISSIFILSIVLLVGLLAP
                     LLKHIEAIIQYIPLCRKISFSLLASLTMWQIYTVLSVLVPLLNILSKNENDCHASNII
                     NKLKGEQN"
     gene            7433..7936
                     /locus_tag="ACFM4S_001144"
     CDS             7433..7936
                     /locus_tag="ACFM4S_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC6050839.1"
                     /translation="MSTKFNQFINRVLSHEGGYVSHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            7950..8123
                     /locus_tag="ACFM4S_001145"
     CDS             7950..8123
                     /locus_tag="ACFM4S_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050840.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            8048..8380
                     /locus_tag="ACFM4S_001146"
                     /pseudo
     CDS             8048..8380
                     /locus_tag="ACFM4S_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8377..8787
                     /locus_tag="ACFM4S_001147"
     CDS             8377..8787
                     /locus_tag="ACFM4S_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6050841.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMAALKKLDALIYGKEVRQKERNSSQG"
     gene            8792..9340
                     /locus_tag="ACFM4S_001148"
                     /pseudo
     CDS             8792..9340
                     /locus_tag="ACFM4S_001148"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9356..9541
                     /locus_tag="ACFM4S_001149"
                     /pseudo
     CDS             9356..9541
                     /locus_tag="ACFM4S_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(9860..10222)
                     /gene="ssrA"
                     /locus_tag="ACFM4S_001150"
     tmRNA           complement(9860..10222)
                     /gene="ssrA"
                     /locus_tag="ACFM4S_001150"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10285..10890)
                     /locus_tag="ACFM4S_001151"
     CDS             complement(10285..10890)
                     /locus_tag="ACFM4S_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6050842.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKPPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2771 a         2532 c         2785 g         3091 t
ORIGIN      
        1 aattaggcgg tatttggggc gaacctgtgt ttgggtttcg gctgtcggga gggaaaggaa
       61 ttttgcaaag gtctcagagt gagtttattt tggggcggcg gcgggtcggg ggcaagcggc
      121 gtggggcttt taggtttggg ggtgggtaaa aaatgccgta cccggcgggc aggcttcgca
      181 ctctgaaggt ttcagacggc gtttgttttt tctatccaat cgaggaactg ccgccatttt
      241 tccagcggca tatcggcccg acgggtttcg gtatcgggtt cggcttccca gcggcctacc
      301 tgtatgtagt gcttcacccc gacgatttgc gccaactcgg cctgtgtcag tttgcagcgg
      361 ttgcgaaggg tgcgaaggtt gtaaggcgtg tagccgagtt ccatgtcgtt gcggtttatt
      421 ttgtttcgca tatttttttg actgccctgc ggcaggtttc ggtaaggacg gcggcaaatc
      481 gggcttcgtc tgccggtttg ccgtcgaaaa aaataaaatc gtaaagggtg atgccgtttt
      541 cggaatggcg gtaatgcccg cccgggcatt cgccgtctgt ctgcctgtgg attttggcaa
      601 tcaggcgggg atagcggcaa tgggtaatga aacggcttgc gccgtcttgc ccgataatcc
      661 attcggggta gcggttgaat agtgcggctc tgttcatttt gttcgggttg ttcgggggat
      721 aaagcccctc gcggggcttg tggtcaggca aatttgaata tcagtgccaa cacggcggcg
      781 atggcggtaa ccagcccggt ggccgccatc atgggatacc atcgggattc ttgggctatt
      841 ttacggattc ggcgttgatt ttgtgcgcgt ctgcaatgat tttggcgatt tcggcatcta
      901 cttttctcag actggagtgt ttcatttctt gttcgattat gttcatcgta cttcctttcg
      961 tttttggcgg ttgccgccgc ttgtcggatg gtaggatgtc tgccatgtgt atatattgat
     1021 accttttagg ttttattgca agtgttttgg gcggcggctt cgtatgcttg gcggtggcgg
     1081 cggctgtacc attcggcgag ttcgcgccgc tcttggaggc ggtagctgtc ggcggtcagg
     1141 tagtggtgca ggcttgagaa gccggcgcgt tggagggcgg cgcggctgat gtggccgagg
     1201 gcgaggtctg ttgtgagggt gtcttttccg gcggtcaggc tgatgttggt gaagaggtgg
     1261 cggaatccgt gcattgtgtg tttggatttg ccgggggtgc tgccgtcata gcccagtcgt
     1321 cggatggcgt tgtgggcgaa tttgatgctg atgtggtcgg gatggggggc gggtttgcgc
     1381 cgtgggcgga tgccggggaa aaggtggatg ttgtcgccgg tctgtgtgtg cagctctcgg
     1441 agtatttcta ccgcccagtc cgacagtggg acggtaaagg ggtgtttggt cttcatgtcg
     1501 gcggcgggga tgtgccataa ccgggcggtg aggtcgatgt cttgccagcg ggcggaaagc
     1561 agtgcggagg gacgggatga cttccccggt ttccccatca atccgcgccg cctcgatttt
     1621 tccgttggcg cgcagcattt ccaccgcctg ccgcaccgac atcaaccgct tgccgttgcg
     1681 cctcatcgcc tccaccaaaa ccgccgaaat caatttggct tcttccggtt taagctccgt
     1741 cttgcccgca tcgctgcgcc gtttgcgcgt cggcttgacg ctgaccgcct ccagcttgcg
     1801 gtatagcgtg gcaaggctga tgcccaattc ctgcgcctgc tgcttaagat atgcagagcg
     1861 tgcgccgcgt tccattgctt ccgcctgatt ctcgactgcc ttaagacgct caatcattgc
     1921 cggattcatc gccttctccc gtttcaccgc ccaaccattc cggcacattg tctgtcggtg
     1981 cttcagtcgg cagggcatag ctttcgcgca gttgctcgca gtccaaaata atttgattga
     2041 gcgtgccgac catctttgcc tgatggctga tctcgtgtgc ctcactgtgc gcattaagtt
     2101 ggtcgaacaa atctttcaaa cggctcactt gactgcggat accgacctca aggcttgtta
     2161 actgcatcgc caactcactg cccacgtctt ccgccttcgg ctctctgaca acggtttgct
     2221 tcttagccag cttctcggtc agctcatcaa ttttggctgt tttggttttc atcacttcgt
     2281 ctttggcggc gaggttttca cggctttcgc gcagggcgac gcgcagctcg cgcaccgtca
     2341 ttcggtccac atcgtcaaag gtcatgccgt tgacttcttc tccttcggca agtcccacca
     2401 acgtaacgtc ttcttcgacc aagagttcca gcagctttga cttgcccaaa tccatcagct
     2461 tcggcgcggc tttctgcatt tgcggcgtgg cgaaacgttg ggtagcagac atcaatcggg
     2521 cggtttcgtt atggccgata ccaaattgac cttttacaat ttccataaat cgcccgtgtt
     2581 ctgtatgctc tttcagtaca ataagtgcgc gccccaattc aaacatgcct tccatcgtct
     2641 gccgtaccgc caagcggccg cgttcgatcc aagtggcttc gttgtaaacc tcgccgttac
     2701 cccattgctc catcaccatc acgctgtgcg cgacttggta gttttgcact gaaaccgcat
     2761 ccatcacttc aacttcattg ctcatttttc actctccaaa tttccttacg tcaggaaatt
     2821 tcaaaattcg ttaatctgca ttcacccgct tgccgatttc ggcaatcttg ctttgcagcc
     2881 gttcatgctg ccgcctgaac cgctcggcga tttgcagggt tttgataccg taggcgtagt
     2941 tgccgttttc aagcttgatg accaatcccg atgcaaccaa atcatcaata tccctgctga
     3001 cttgtgatgg cgtcagcccc agtccgaccg ataaatcctt attgctcaga ccgataatcg
     3061 gatgttcgtc aagcgcgata aagaccctca atagccgttg taccctttta ctttctgcca
     3121 tccgcatcct ccttatttca gtcccagctt cttggcaatt tcgtacccct tgccgtaatt
     3181 gcctttgcgc tgtccgccga tcactagata cacatcgcgc ggcttaaagc cgttctctct
     3241 tgcccactgc gccagcgtct ggccgttttg ggcaaaattt tctttcaatt cgtctgccgt
     3301 aagcatttct ttggcctgta cgcgggaaaa tttcagtatc cgtccgtacg gttggttaat
     3361 acactcatga ttaatcatcg cttttctcct tcatccccag caaaaccgcc gcttcgtggc
     3421 ttttgccaaa attgcctttc agcttgccgc gcaagaggtg ctccaccgtg gtgcgctcta
     3481 gattgaaata tttcgcccaa tgcgccttgc acaccccgtt gcgcttaaac cacccggccg
     3541 cgctctcgcg cgtttgcgga tagggaatag gcttgaaatt caaaggtttt tccatattat
     3601 ttatgccttt cgtgtgatat aatgtgtttg tttttaggtc tctttgcacg ataaacatcc
     3661 ggtctgttgt gcagcagcag ggtcttggct tgttttaaag taaacaagct ttttctcgta
     3721 gctgggtgta tgtgatccat catgagctgt cgcgcatgag ctttcaaagt gttcattttt
     3781 tcgtcctttc tcgtgatgat ttagggtgtt tgtgtttcga tgtggaaatt ataggaagaa
     3841 ttcttcctat tttgcaagga atatttatga ataactcttc cttttttggc aaccgattga
     3901 aagaagaaag aaaaaaatta aaaatgactc aagctgaaat cgctgaaaaa tgtggggttt
     3961 caggaagaat gtggggggat tatgaacgtg gcatcagcca gccaaaagcg gaacttttct
     4021 tccaatttga aaaggtgggt atagacgttc aatacgtcat gcacggcaga cgcggcgaaa
     4081 cagcggtcat gccgtctgaa accctgaacg ccgaagaaca agaactgctt gccctgttcc
     4141 gcgaggcggc agctgccgac cgtgaaatga ttctgatggt tgcgcgcagg gcagagaaaa
     4201 aagcccaaac tgcgcttggt aaagtgagta atggataaaa tgaatcaatt cgaattgtgc
     4261 caaaaagaac atgtcaatcc atttgccttg tcaaagcaat atctattggt tgtaacattc
     4321 gtcaaatcca gcagtaaaaa ttttcaggca gcattacttt gggcaagaag tgccaaatta
     4381 tttgagaatc ttgagattgg aaaagaaacc atctattgct gcgctttcga taaaacagca
     4441 gaacaggctg ggatggccgg ggtatttttg aattatattg aaaattggaa tggcaaacag
     4501 atttacatca atggccgaat ccatagtggc agtatttatg atttgttagg ggttttagac
     4561 tgctatcaaa aatcacagtc ctgtcccaac cctaaaagcc actgttgctt tgtttcagac
     4621 gacatttttc tatggcatgg atcaagacca acgtttgaaa tcagtcttga tctaactgga
     4681 aagaaaaaag aaacatcctc tgcaaagaaa tttgtgatgc cttgtattaa tttccgtcac
     4741 cataggattg aaaaagaaac ctacttagga aattggaatg aacaaattgc cgcattggca
     4801 gtaaaacaaa atatagattg gtgtccaagt tttgatattg agaattttag acagtatgaa
     4861 taattactat ctatatagga attgcagcag cgatgtgtta tgggtcaaac gtatccaacg
     4921 ccaaatcgac ggcagcctac tcttgatttc tgacaattca acctatccac ccatgccctt
     4981 ggcactggcg gaacaccccg atattcaaat catcgggcag gtagtgcagg tatcaaaaga
     5041 cttgaactag acacaatcaa aaagggaaat agaatgaaaa tactcgcttt attaattgcc
     5101 gctacctgtg ctttatctgc gtgtggcggc caagctgaag aacaaccggc atctgcccaa
     5161 cctcaagagc aggcacaatc cgaattaaaa accatgccgg taagctatac cgactatcaa
     5221 tcagtagcca ataaagggct tgccgaccaa aaaactggtc tgacccttcc agaacatgtt
     5281 gttcctgccg acaatgcgga agggaagaat ctgctgcatg acttttcaga cggcctcaca
     5341 ttaaccgttg ataccgataa agccgacaaa attactgctg tccgagtagt ctggaataca
     5401 gatgcaatgc ctcaaaaagc ggaaaaactg tccaaagctg ccgcagcctt gattgcggca
     5461 accgccccgg gagaccgcac actgctgcgt gataccggcg accaaatcaa aatggcaatt
     5521 gacagccata atgcgcaaaa agagccaacc cgagaatggg cgcgtggtgg gattgcttat
     5581 aaagtcactg ttactaattt gccgagcgtg gttttgacag caaaagctga ataacctgca
     5641 ttacagccac aattaggagc agacggagat gatgtttata gagcctgctg agaataaata
     5701 actgataaaa aagctgtctg aaaaggtttc ggatagttag gatagctatc tatttttaat
     5761 atattagaga gaatatgaaa aataaactga ctttacgtta tttcaaaata actcgttgcg
     5821 gttattggtc tggttcttca aaagaaccta atcattatga ggacttatta aaaactttaa
     5881 ctcatttaga gacatatgtt aaaggtaaaa ctattgaaga gactggaatt agtcgtgatg
     5941 ataaagagcc aagcactttc ttgctcgata tctgcaaaaa aaatggtctg tggttgcttg
     6001 ctttttggaa tcctgccgca gaaagctctt cagatgaaat tgtttcttta aacatgaaaa
     6061 gtaaactagg tcaagttgat tctagcccag taacagcagg aaatgggaaa acttttggct
     6121 tcgttagtta ttttcttatt tggccagaac ataattttta ctcaacgatt ctttctccta
     6181 atgcaacttt agctggacga acagagtttg aagagtacat gcttaatttc ttgtggattg
     6241 atcctaattt tatagaaaag actacctacg aagaaacttc aacagacgga ataattcata
     6301 ccatggagcg aactaatttt aaattgcctc ttaaaattaa tggaactcct gtgccacgtt
     6361 ttgcggggaa actagctaaa tctccttcag aaaaagagta tgtcattagt aattatgacc
     6421 aagtaaccca agtgcataat caatgcacgg taaaggttac ctctggacaa agttcaagtg
     6481 gaattttgaa gaaaatgtta ccaagctttt ttggttcaac agaattgttt gctccattaa
     6541 aaaccattcg tgtaaatcat tctgtttctg ctgcatttga aaacaaatca aatcttaagg
     6601 attggattga agactgggaa aaaaattctt ttgatttaga taaagatgat tgtggcttta
     6661 agttaaaaaa cgatgacaag atttattgga ttcgaggaaa tgtcatcaaa tgtgaattag
     6721 acgtcaacct tcataaggaa aatgggattt atccagctgc taaaattcta gatataatag
     6781 accaaaggaa agattacgtt ttaaacgaat tcaaattaag tgatgattct tagaaatatt
     6841 ttcctaatat gctctgcatt catgattggt gtagcctggt atttaggtgc taatatccga
     6901 tttgaagagc aatgggtatt gtatgaagcc ttgagaacaa ctgcatcaat tatttttgca
     6961 gttgttgggg tttggttggc tattgtctat cccgaacgat taaaaagccc ttttagtcaa
     7021 actttctcct ctccggtata tcggcgtgga ttcaaacagc ttttttcccc tgttataagt
     7081 tccattttta ttttaagtat tgtgttactt gttggtttgt tggctccttt gctcaaacat
     7141 attgaggcaa taattcaata tatcccactt tgtcggaaaa tctcatttag cctgttagca
     7201 agcttgacga tgtggcaaat ttatacggtt ttatctgtat tagtgccttt gttaaatatt
     7261 ttaagtaaaa atgaaaatga ctgtcatgca tccaacatta ttaacaagct aaagggtgaa
     7321 cagaattagc tatgtgattt ttagtgtaaa ctgcttttaa ctcgtattaa aaggccactc
     7381 ttttaatacc tcctaaaatc cctgtaccta tcaatagata cagggatttt ttatgtccac
     7441 caaattcaac caattcatca accgcgttct ctcccacgag ggcggttacg tcagccatcc
     7501 caaagacccc ggcggcgaaa ccaattgggg catcactaag cgcaccgccc aggcaaacgg
     7561 ctacaacggc tccatgcgtg ccatgacgcg tgaacaggct atcggcattt accgaaaggc
     7621 gttttgggag cgttaccgcg ccgaccaaat gccggaagcg gtcgcgttcc aattttttga
     7681 tgcctgcgtc aaccacggtt acggcaatgc cgtccgtatg ctgcaacgcg ccgcaggcgt
     7741 ggcggacgat ggaatcatcg gcgaaatcag cctcaaagcc atcaattccc tccccgaaaa
     7801 cgacctttta ttgcggttca acgccgagcg tctggtcttt tataccaagc tcagtacgtt
     7861 cacctctttc ggcaagggct gggtacgccg tgtggcgcaa aacctgattc acgcgtctgc
     7921 agataacact gattaaaggg agataaacca tgtcaaaaaa gtcactcatc gccctaatga
     7981 ccgcagccat gcagcccgat ttcagccaca gcgacctagg cattcgctac gccatgccga
     8041 ctcagggatg ttggacgcaa gcccaccgca agagcggggt agccgccgcg aaacgcgcag
     8101 ccaaaaaaaa gcgtcacaaa taaccgcctt tttccgatgg ctgggcggct tggtctctaa
     8161 tccggccaca ggaaaaatca gccataccaa actatgggca aacgtcgccg cagccgccat
     8221 gacttggaag ttcgtgcagg cggcggacgc gcccgaatgg ctctagtggg cttatggcgc
     8281 attggtcggc gggtatgcat taatcaaacg cggcatcgcg gcgattccgc agttggcaga
     8341 aatcaaaaaa tccgcaaatc aggaaggggg gcggcaatga ttgaatttgt ccgagccaaa
     8401 aaacggctgc tttgggcatt tgtgcttttg cttgtgtgga cgtgcggtta ccgatacgcc
     8461 gccgacaagg ccgaagcgaa acaaaccgcc ctgattgcca cctatcggca ttcttctatg
     8521 gttgcggcgg aacaatacgc cttgcagctt aaaaaagcgc aggacgaaag gcagcggtgg
     8581 tacgactttt cccaaaaaca aggaagaaag cccgtgaaaa aacagtatcc gccgcaaacg
     8641 aaaaaagccg gctatctgaa aaccaaggaa gaactgcttg cggaattggc ttgccttaaa
     8701 gcggaaatgg ctgccctaaa aaagctcgat gccttaatct atgggaaaga agtgcggcag
     8761 aaagaacgca actcgtcgca gggttaaggc aatgccatcc gttgaactgc tgttggtgat
     8821 tgtcggactg ccacgcggca ccttctatta ccaattggcc gtccaatcgg cagaagacaa
     8881 atatgccgat ttgaaacggc atatccatga tatttatcga cgacataagg gaagatacgg
     8941 ctaccggagg attgcggcag ccatccgtca cgcaggaaca ccggtcaatc acaagaaagt
     9001 cagccgtctg atggcgaaga cggggctgaa ggcagtgata cggcggcgca aataccgctc
     9061 gttcaaagga gaagtcggca aaattgcgcc gaatatcctg cgacgctgtt tccatgcaga
     9121 aaagccgaat gagaaatggg taacggacgt taccgagttc aatgtaggcg gagaaaagat
     9181 atacctttct ccgattatgg atttgtttaa cggggaaatc gtcagttacc gtattcaaac
     9241 ccgcccgact ttcgatttgg ccgacgagat actgaaaggt gcgccggaga aaccgggatc
     9301 gtctgaaaag ccgatactgc attcggatca aggttggcaa tatcagatgt ttttttatag
     9361 tggattaaat ttaaatcagg acaaggcgat gaagccgcag acagtacaaa tagtacggca
     9421 aggcgaggca acgccgtact ggtttaaatt taatccacta tacaacaacg atagaatcaa
     9481 gttgaaatta aaagggctga gccttgttca gtacagaatt cagtccctga aagccgcttg
     9541 attaaactgt ccgacttttt ggggtcagtt cggcttcggc atttttttat ccgtttgggg
     9601 gtaacttgtt tggaaagctg caagcttata aataaaggat tacatttaag ttttgggtaa
     9661 cctttttaaa aaaatgcgtg atgacttttg catttttaag gcgttttttg gggtaattcg
     9721 tgaaaagtta ccccaaaagt taccccataa atggcgaaaa ctcaagcata cgccagcata
     9781 ttcaaggcaa caaaaaagcc ttgaaactgt tgaagttcaa ggcttttttg tgttgcagga
     9841 tgctgctgaa aatagggtat ggtggaggcg gggggaatcg aacccccgtc cgaaagtcct
     9901 ctacaaagcg ttctacatac ttagttgtgt ctatttgaaa atcttatttc catcatgccg
     9961 accaacaggc cttttggaaa ccagttacct taaatctcat ttcctgccaa gtaacccggt
    10021 aggaaaccag tcaatgtaag atgacgttgc ggtggctttc gccacacagc ccattgaccg
    10081 actgctgcaa cggctagcct taagcggcta aagcgtaagt ttcgtcgttt gcgactattt
    10141 gaattcagtg ttttacggga atctgagacc ccggtatgcc cgcatctgct tcgcaacccc
    10201 cgtcgaaacc aaggtcgccc ccagaaatgg tttgcaaatt atacggatat tgtgcggtgc
    10261 tgccaagtct gtcggagaaa tttgtcagtc ttgctgcctt aatttgcgtt tgagcaggat
    10321 gcggacgcag ccgtcgttgc cttcccgggg ttcggcgtag gcgagtacgt cggggtgttg
    10381 catcagccag tttcgggtca tatttttcag aacgggtttg tagcctttgg aacctaatcc
    10441 gctgccgtgg atgatttcgc cgcatacgcc gcgtttttgg gtgaatgcga tgaattcgtt
    10501 gaggactttt tgggcttctt cctgtgtgta gccgtgcagg tcgacatcgg taacgacggg
    10561 atagtatccg tttttcaggc gttggatgtc gttttttccc tgtccgtttt tgctgaagct
    10621 ggcgggcggg tcgttgtatg tgctgccgat gtagaagtag ttttcttcgt cggcgcggtt
    10681 gtctttggga cggactttga tggggggttt gtcgggcggc gcataatatt gctgccggtt
    10741 ttttaatggg gagacttgtc cgactgcttg tgaaaaatcg aaatcctgtt cttgtttttg
    10801 cttgtttttt tcggctgcct gtttttctgc cgcttctttt tgggcttgtt tgcccagttg
    10861 tttgaggatg ttttggaagt cggtattcat attttttcct gttatttgtc cgatggctgt
    10921 ttcgggcggg gttttaattt gccggaatgt ttgccaatcg ggggaggatg attttgttgc
    10981 ctgcgtatgt tttttgaaag tgtgattgta tatcaaaaag aaatgcggca accgtcggca
    11041 gtgttgattg ccggaaatgc ggaccggtcg aaccgatatg cccgaacgcc tgataaagtt
    11101 ttaaaaacct gccttgcgaa gcaggctgac gtgttttgcc aatcttgaat tgccggaaac
    11161 gcgaaacacg gaaatctga
//
LOCUS       DAXHMX010000061        11155 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHMX010000061 DAXHMX010000000
VERSION     DAXHMX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.061"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            40..463
                     /locus_tag="ACFM4S_001152"
                     /pseudo
     CDS             40..463
                     /locus_tag="ACFM4S_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            569..1132
                     /gene="pgsA"
                     /locus_tag="ACFM4S_001153"
     CDS             569..1132
                     /gene="pgsA"
                     /locus_tag="ACFM4S_001153"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6050843.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1212..1287
                     /locus_tag="ACFM4S_001154"
     tRNA            1212..1287
                     /locus_tag="ACFM4S_001154"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1245..1247,aa:Gly,seq:gcc)
     gene            1313..1388
                     /locus_tag="ACFM4S_001155"
     tRNA            1313..1388
                     /locus_tag="ACFM4S_001155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1346..1348,aa:Gly,seq:gcc)
     gene            1412..1487
                     /locus_tag="ACFM4S_001156"
     tRNA            1412..1487
                     /locus_tag="ACFM4S_001156"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1445..1447,aa:Gly,seq:gcc)
     gene            1509..1584
                     /locus_tag="ACFM4S_001157"
     tRNA            1509..1584
                     /locus_tag="ACFM4S_001157"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1542..1544,aa:Gly,seq:gcc)
     gene            1611..1684
                     /locus_tag="ACFM4S_001158"
     tRNA            1611..1684
                     /locus_tag="ACFM4S_001158"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1643..1645,aa:Cys,seq:gca)
     gene            1787..1876
                     /locus_tag="ACFM4S_001159"
     tRNA            1787..1876
                     /locus_tag="ACFM4S_001159"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1821..1823,aa:Leu,seq:taa)
     gene            1980..3032
                     /gene="sohB"
                     /locus_tag="ACFM4S_001160"
     CDS             1980..3032
                     /gene="sohB"
                     /locus_tag="ACFM4S_001160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6050844.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3083..3950)
                     /locus_tag="ACFM4S_001161"
                     /pseudo
     CDS             complement(3083..3950)
                     /locus_tag="ACFM4S_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(4037..4906)
                     /locus_tag="ACFM4S_001162"
     CDS             complement(4037..4906)
                     /locus_tag="ACFM4S_001162"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6050845.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(5001..5918)
                     /gene="hisB"
                     /locus_tag="ACFM4S_001163"
     CDS             complement(5001..5918)
                     /gene="hisB"
                     /locus_tag="ACFM4S_001163"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6050846.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(5915..7012)
                     /gene="hisC"
                     /locus_tag="ACFM4S_001164"
     CDS             complement(5915..7012)
                     /gene="hisC"
                     /locus_tag="ACFM4S_001164"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6050847.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(7058..8347)
                     /gene="hisD"
                     /locus_tag="ACFM4S_001165"
     CDS             complement(7058..8347)
                     /gene="hisD"
                     /locus_tag="ACFM4S_001165"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6050848.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(8344..9243)
                     /locus_tag="ACFM4S_001166"
     CDS             complement(8344..9243)
                     /locus_tag="ACFM4S_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6050849.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(9347..10000)
                     /gene="hisG"
                     /locus_tag="ACFM4S_001167"
     CDS             complement(9347..10000)
                     /gene="hisG"
                     /locus_tag="ACFM4S_001167"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6050850.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(10079..10732)
                     /locus_tag="ACFM4S_001168"
     CDS             complement(10079..10732)
                     /locus_tag="ACFM4S_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6050851.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            11009..11093
                     /locus_tag="ACFM4S_001169"
     tRNA            11009..11093
                     /locus_tag="ACFM4S_001169"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11043..11045,aa:Leu,seq:caa)
BASE COUNT         2473 a         2659 c         3163 g         2860 t
ORIGIN      
        1 tatcgctggg tatggtttgc cgccataagc tgtctgctgt tgtccgccgt ttttattgca
       61 ccttacctga cggcatttca cgaacaagaa aaaacattcg aatatgcgga attgaccgtt
      121 accgccccca accgcagcgg acgggcaatc aaactggatg cagacgggca gcattaccgg
      181 ctttcctgct atgggttcga cggtttgtgc gcggaaggca atatcggcag aaccatcagg
      241 gcggagcagg ttagagccgt tttaagcgaa accgtcggca aaggtttttt aaacggcgtt
      301 ctgttggaat accgcaacag tggcagtgtc tataagcaat aaagattttg cccgcacgga
      361 agaccgcctt gtcgaagtgt tggcacaacc tgccgttttc agcctgaaac tgggtatttt
      421 gcttttattg cccgccattt tcctgcgttt gaaaagaatg tgaaaacaga ttggcggggc
      481 gggggaatgc ccatgcagca attttacgtt ttgggttatg aggataaccg ttataatcac
      541 aactttacag tctgcacaga gaaaaccgat gccttggaat ctccccatct tcctcacatg
      601 gttgagggtc ttgctcattc ctgtcatgac agcccttttt tacctgcctt ttccgtggtt
      661 cgcggaggaa acgattaatc tcaccgccgc cgtcattttt gccgttgccg ccttaaccga
      721 ctggtttgac ggatttttgg caagactgtg gaaacagact tccgatttcg gcgccttcct
      781 cgatcccgtc gccgacaagc tgatggttgc cgtatcgcta cttttactgg tcaaactcga
      841 ccggacctat gttttgtttg ccatgatcat tatcggcagg gaaattacca tttccgcatt
      901 gcgcgaatgg atggcgcaaa tgggcaaaag gaacagcgtt gccgtcgcca ccgtcggtaa
      961 gtttaaaacc gccgcgcaaa tgctggcaat tttcctgctg ctgctgaata ttcccgattt
     1021 ttacggattt aatcttgttg ttatcggcaa tgtattgatg tttatcgcat ctttgctgac
     1081 agtctggtcg atgctgtatt atctgaaaat ggcgtggaaa gaaatcgcct gaaaaaaaca
     1141 taaaaatagc ttgacggcaa aaacagaatc cataataatt gcgtctcttc gatgttggag
     1201 aatgaaatcg ggcgggaata gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg
     1261 cgagttcgag actcgtttcc cgctccaaaa atttcatttt cccacaatca ttgcgggaat
     1321 agctcagttg gtagagcgca accttgccaa ggttgaggtc gcgagttcga gactcgtttt
     1381 ccgctccaag ttttatttca aacatatcaa cgcgggaata gctcagttgg tagagcgcaa
     1441 ccttgccaag gttgaggtcg cgagttcgag actcgttttc cgctccaaaa accgatatgt
     1501 ttgaacgcgc gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga
     1561 gttcgagact cgtttcccgc tccaagtttt tttagataag ccagttttag ggcgagatag
     1621 caaagtggtt atgcagcgga ttgcaaatcc gtctacgccg gttcgattcc gactctcgcc
     1681 tccattatcg tactatggcg gggtggcaga gtgtttatgc tatgaggagt gcaatcttca
     1741 tataggccgg ttaaaatccg cgcccccgcc tccacctttc acaaatgccc gggtggtgaa
     1801 ataggtagac acaacggact taaaatccgt cgggactaaa catcccgtgc cggttcgatt
     1861 ccggctccgg gcaccaagct gaaaatgaca atgccgctcc aagcggttat ttttttatct
     1921 gtcggacggt gatggacaat attgacatgt tcatgcctga acaagaggaa atccaatcaa
     1981 tgtggaaaga aattttactg aattacggta ttttcctgct cgaactgctt accgtgttcg
     2041 gcgcaattgc gctgattgtg ttggctatcg tacagagtaa gaaacagtcg gaaagcggca
     2101 gtgtcgtact gacggatttt tcggaaaatt ataaaaaaca gcggcaatcg tttgaagcat
     2161 tctttttaag cggggaagag gcaaaacatc aggaaaaaga ggaaaagaaa aaggaaaagg
     2221 cggaagccaa agcagagaaa aagcgtttga aggagggtgg ggagaaatct tccgaaacgc
     2281 aaaaatcccg cctttttgtg ttggattttg acggcgattt gtatgcacac gccgtagaat
     2341 ccttgcgtca tgagattacg gcggtgcttt tgattgccaa gcctgaagat gaggttctgc
     2401 ttagattgga aagtccgggc ggcgtggttc acggttacgg tttggcggct tcacagctta
     2461 ggcgtttgcg cgaacgcaat attccgctga ccgtcgccgt cgataaggtg gcagcgagcg
     2521 gtggttatat gatggcgtgt gtggcggata aaattgtttc cgctccgttt gcgattgtcg
     2581 gttcggtggg tgtggtggcg gaagtaccga atatccaccg cctgttgaaa aaacatgata
     2641 ttgatgtgga tgtgatgacg gcgggcgaat ttaagcgcac ggttactttt atgggtgaaa
     2701 atacggaaaa gggcaaacag aaattccgac aggaactgga ggaaacgcat cagttgttca
     2761 agcagtttgt cagcgagaac cgccctcaat tggatattga ggaagtggca acgggcgagc
     2821 attggttcgg tcggcaggcg ttggcgttga acttgattga cgagatttcg accagtgatg
     2881 atttgttgtt gaaagcgttt gaaaacaaac aggttatcga agtgaaatat caggagaagc
     2941 aaagcctgat ccagcgcatt ggtttgcagg cggaagcttc tgttgaaaag ttgtttgcca
     3001 aacttgtcaa ccggcgggcg gatgtgatgt agtttgccga acggatggaa atgccgtctg
     3061 aagcgtgttc agacggcatt tttcaagttc cggctttgat gttccgttcc gaccactcta
     3121 tccgttgtcc gatgtctttc aaattggaat attgttcgac caatcgcggc acgtcgtcca
     3181 aactcatggc aaacatccac agataagtgc cgaccgaata gacatgcccc gcgctgctgt
     3241 agcctttgag cgtcatcatc acaaaagcaa agccgaacaa aatacccatc gccgcgccga
     3301 cgcagagata gccgaaggct tcgcggttgg aaatcagcac acgcaggcgc gcaaccagtc
     3361 cgtaatggcg gtccagctgc cgccggtctc cttttcggat aaagtggttg tcgcattcca
     3421 agctgttctt caggcggaaa tacaggtttt cgctgatggc ggcaaaacgt ggcaaaagcc
     3481 ataaaaacaa cgcaagtatg cccaccgccg acacgccgac ccaaaattcc agcaccagca
     3541 gcatgatgca cgcgccgaat atggatacga cggatgtcgc ggcaatcggc aggtgttctt
     3601 caaaaaagct gacaaactca cgcgacaggg caacccgcgc agttaccgcc gaatgcggga
     3661 cttgtcgctg ccgctgttcc aacacgaccg gcacggcgat ttcggtataa atccgcgtaa
     3721 acgtgcgcgt atcggcaatc cgccgcgccg caccgaccag ccacatcaaa agcacaacca
     3781 aagcgtacag caacgcctgc cacacccccc cccgcaatca cggcattgat cgcccggccg
     3841 ccaaacaccg gatacaccag catcaaaagg ttttccagtc cgaccaggga aaatgtgcca
     3901 atcaatcgct tgcggtgggt ttgggctatg tgtttcaaca ttttccacat atttgttccc
     3961 tattttttat ttagcgtggg gtgataaaaa tgccgtctga aatatgttca gacggcattg
     4021 tgtttggaaa aggcaatcag tgttctgcca atttcaggta aacgccggaa acgtcctgtt
     4081 cgccgtagcc ggcttcgact gctttgcggt agctggcagc aacggtttcg acggcgggca
     4141 gggtgttgcc tgcctgttca agctctttga cggcgaggtt gaggtctttg gaggcgtgtt
     4201 tgagggcgaa ggcgggtggg aattcgcggt ttgcccacag ggattttttg gtttggaaca
     4261 tgggcgagtc cattgccgag ccgccgatgg cttcgacgat ggtgtcggta tcgatgccga
     4321 actgccgcgc catcagcatc gcttcgctgt acgcttcgcc gaaaatgccc aagagcgagt
     4381 tcaagacgag tttcgcgccc gaacctttgc cgacatcgcc gaaatggaag gtttttttgc
     4441 cgacgaggga aaatattttt tgcagcgggt ttaaaacggc ttcgctgccg ccgaacagaa
     4501 tcagcagcgt gccgttggtg gcgggcccga ccgatccgga aacgggtgct tcggcaaact
     4561 gtccgcctgc ggcttcgaca agtgctttga cggcgaggtt ttcggtcggg gagatggtgc
     4621 tcatgttgac gatgattttg ccggccaatc cgtcgcggac tccgttcagg atgtcgcaca
     4681 cggcggcata gtcggaaacc atcaggaaaa tgacgggata gtcgcggacg agttcggcgg
     4741 tgttgccgta aacttttgcg cctttggcgg agatgggggc agttttgtcg ggcgagcggt
     4801 tgtatacgcc gacttcgatg ccgccgtcca agagccgcgt taccataggc agacccattt
     4861 gccctaagcc tatccagccg atttgtgtgt attcgtttgc ggacatgatg tttactcctt
     4921 ttctgtttgt agtgtagtgt aatcttatct gttccgatac cgtctgaaaa gcctgcgggt
     4981 gtttcagacg gtatggtttt ttatgcggtc agcgtgcctt tggtcgaggg ggtctgtcct
     5041 gccatgcgcg ggtcgtgttc gactgccata cgcagggcgc gcccgaaggc tttgaatacg
     5101 gtttccgcct gatggtgggc gtttttgccg ctgaggttgt cgatgtgcag ggtcatcata
     5161 ctgtggttga cgatgccgtg gaaaaattct tcaaacaaat cgacatcgaa acgtccgatt
     5221 agtgcgcggg taaattcgat gttgtacacg agtccggggc ggccggaaag gtcgatgacg
     5281 acgcggctga gggcttcgtc gagcgggacg taggaatgtc cgtaacggcg gatgcctttt
     5341 ttgtcgccga gtgcctgccg gattgcttgt ccgagtgtga tgccgatgtc ttcggcggtg
     5401 tggtggtcgt cgatgtgcag gtcgcctttg cagctgatgt cgatgtcaat catgccgtgg
     5461 cgggcgattt gatcgatcat gtgttcgagg aagggtacgc cggtatccag cctgcttttg
     5521 ccgctgccgt cgaggttgat ggagacggtg atttgggttt cgcaggtatt gcggttgacg
     5581 gtaacgctgc ggcagccggc agcagatgcg gtttcggcaa tttgggtttc ggcagttccg
     5641 gtgctttctg cggcggtttc ggggacagtg cgttcgcggt gttttctgtc gagccagcct
     5701 ttgggtttgc cggtgtgttt ttcgagtttt gccatcaggc tgggacggat gccgcgtgca
     5761 tctgggtctt ctgcctgctt ttcaaggcgt tgtttgagtt tgtagagtgc gacgggggta
     5821 cggtagccgc agagtttggc gagcttggac agcgaacctg cttcttgggc gagggtcaga
     5881 aagttgtgca gttggcgttg tgttttagtc aaattcatag gaaatccgtt ggttggcagt
     5941 aaagttggcg aatgatgttg agaacggcat cgttttgtgc ggggctgcct acggtaatgc
     6001 gcaggcagtg ttccaaaagc gggtgcgcgc catgcagttt tttaaccaag atgcggtttt
     6061 gtttgagcgt gtcaaacaac aaatcggcat cgggtacgcg tatggtaatg aagtttgcct
     6121 gacttggaaa ggtgttcaga cggcatattt tgcccaattc ggcgaacatc cgttcgcgtt
     6181 cgtttttcag gctgtcgatg ttggcagaga tgatgccgta gtgttgcagg gcgagtttgg
     6241 cggtggtcag gctcagttgg ttcatattgt agggcggcag gattttttgc agttcgccga
     6301 tgacttcggg acagcctgcc gcatagccga tacgcagtcc ggcaaaaccg attttgctga
     6361 cggtacgcat gacgatgagg ttgggaatgc tgcccgcctg cggcaggaag ctgtcgccgt
     6421 tgaatgcgcc gtaggcttcg tcgacgacga cgatgccgtc tgaagcttcg atgacggctt
     6481 cgatttcggc acgcgtgaaa catacgccgg tggggttgtt ggggtaggcg ataaaggtca
     6541 gggcagggcg gtgcttcctg acggcttcga ggacggcggg caggttgagg gtgaaatctc
     6601 cgttcagtgg aacgccgaca taatccatgc cgtacagcgc ggcgttgtgg cggtacatga
     6661 cgaaactggg ttcggctgcc aacattgccg cgcccggttt ggcggtcagc atcgtgatga
     6721 actgtatcag ctcgtccgag ccgttgccca gcgcgatgtc ggcgcagtcg ggaatgtcga
     6781 acgccgaacg taatgcttcc tgtaaaccgc tgccggaggg attggggtaa agatggatgg
     6841 gcgcggcggc aagccgtgcc tgccattcct gcaacagcgt ttcatgtccg gcaaaagggt
     6901 ggacgggact ttccatcgaa tcgagtttgg caaagccggg cggaacgtcg gcaatctgat
     6961 atgccgacat agcttgtatg tcgtcgcgga tgaaggagcg gacggatttc atcgtgtttc
     7021 cttaatggtt ggaatatgcc tgtacgccgt ttcggcatta tttcatacgg aactctgccg
     7081 cgcgggcgtg ggcggtcagg ctttcgccgt gtgccagcac gctggcggtt tcgcctaatt
     7141 tttgcgcgcc ctgttccgaa acctgaatca ggctggagcg tttttggaaa tcatatgtcc
     7201 ccaaaggcga ggaaaagcgg gcggttcggc tggtgggcaa tacatggttt ggaccggcgc
     7261 agtagtcgcc gaggctttcg ccggtgtagc gtcccatgaa aatcgcaccg gcgtggcgga
     7321 tttttttcgc ccattcctgc ggattttcga ctgacagttc caagtgttcg ggggaaatgt
     7381 agttggcgat ttcgcaggct tcgtccaagt ctttggcgag tatcatcgcg cccctgttgc
     7441 cgagcgaggc ttcgatgatg tcgcggcgcg gcatagtttc gatcaggcgg tccatagcgg
     7501 cttctacttc gtcgagatac gcttgcgacg tgccgatgag gatggcttgg gcaatttcgt
     7561 cgtgttcggc ctggctgaac aaatccatcg ccacccaatc ggcaggtgtc gtgccgtcgg
     7621 cgatgaccag gatttcagac ggccccgcca ccatgtcgat gccgaccacg ccgaacacgc
     7681 ggcgtttggc ggcggcgacg aaggcgttgc ccggaccggt gattttatcg acttggggga
     7741 tggtttccgt gccgtaggcg agggcggcaa ccgcctgcgc gccgccgacg gtgaagactt
     7801 tggttacgcc ggcgacgtat gcggcggcaa gcacgatgtc gttgcgttcg cctttcggtg
     7861 tcggcacgac catgatgatt tctttcacgc ctgcgacgtg ggcgggcatg gcgttcatga
     7921 tgacggaact cggatatgcc gccttgccgc cggggacgta aatgccgacg cggtcaagcg
     7981 gggtaatctg ttgtcccaac agcgtgccgt cttcatcggt gtagctccac gattccattt
     8041 tttggcgttg gtggtagctt tcgacacggc gggcggcggt ctgcaatgcc gtctgaacgt
     8101 cgttcggaat gcgctcgaac gccgcgttca aatcggcttg cgtgagtatt aaatcatcga
     8161 tgcttttagc gtttgtctga tcgaatttgt tggtgtattc aatcaaagcc gcatcgccgc
     8221 gcttttgcac gtcggcgcaa atgtcggcga cgatgcgttc ggtttcgggg ttttgcgcgg
     8281 tttcaaaagc cagcagggct ttgagtccgg cttggaaatc gggcgattgg gtgttgagtt
     8341 ttttcatgat ttggtttttt ccttttttat ttttggattg ggggtcgtct gaatattctc
     8401 ttgttcagtt tctgtctctg ttttgggtct gatatacgct ttttctcccc cgctaccaaa
     8461 cttcaattcg ggcaggcatt tttgttgcag accgagtttg gctaatacct cttcgctacg
     8521 agaggtagtg cggaaacggt aacgggattg ttttctaccg tttttggttt cctcaaccat
     8581 tgtttcaaaa ccgtccactt cgtatgcact gacaacagcg ttttgcagac cggtgtgaac
     8641 gccgataacg tatttcactt ttgaagcaac atctttaccg ataacccagt tgcccaacgt
     8701 acgcgatttg aggttggcat cgtcttggtt gtcgaaaagg tagtctaatt cttcgtcagt
     8761 atctaaatcg aaagcattgt ggattttgat ggcgagaatc agcccgtcgg ggttaatctc
     8821 gttaagtggg agagaagaga atccaaagcg gcgatctagt tcttctacgc taataccacc
     8881 cggaccatgc ccggcaattt tatttttgag ttttttaccc aagacagatt taacaaaatg
     8941 aattaaggca gattcggcgg cgagtgcttc gacttcagtc agatgatagc tgatgatata
     9001 gcgaccgagt ttcttgcatt tggagatggc tttgagtttc cgatcgataa tctcgccgga
     9061 gactgggtct tgtgaacgac tagcaaccca ttcatgctcg aagatacgat taccgcagcc
     9121 tttgccgatg tagaggattt tgtctttttt caggtcggtc agacaataga cataaaaccg
     9181 acgctcgccg ttattcaaaa cggaaagggt tgaatctgag aattttttta tttttgcaac
     9241 cattttaact tctcctatac agtttactgc cggtgatatt ttcagacgac ctgtcggcgg
     9301 gaacggatag ggtcgtctga aaacgcatct ttattcaaat ggatgcttac ttcgccttca
     9361 ctgcgccgcc gaacgcctga ataatcggct ccagcagcgc gtatttcgtt ttcaaagcag
     9421 ccttgttgac caccaggcgg ctggaaatgt cgacgatgtg ttcgactgct tccaagccgt
     9481 ttgccttcaa ggtgttgccc gtcgaaacca agtccacaat cgcatcgctc aagccgacca
     9541 gcggcgcaag ttccatcgag ccgtacagtt tgataatgtc cacatggaca cccttgccgg
     9601 caaaatgaga tgccgcgatt tcaggatact tcgtggcaat cttcagacgg catccgggtt
     9661 gcgaagctgc ttcgtaatca aaccctttac gcacagcaac catcatgcgg cacttggcaa
     9721 tctccaaatc caaaggccgg taaagccccg tgccgccgtg ttcgatcagc acgtctttgc
     9781 ccgcaatgcc gaagtccgcc gcgccgtagc ggacataagt cggcacatcg gttgcgcgga
     9841 caatgacaag gcggatgttt tcatggttcg tcccgataat cagcttgcgc gatttttcag
     9901 gctcttcagt cggaacaatg ccggcagcgg caagcagcgg cagcgtctcc tcaaaaatgc
     9961 gccccttgga taaggcgatg gtcaaagcat tatcctgcat ggtgtggatg tcctttgtcg
    10021 gttgaaacaa taatgccgtc tgaataggcg ccgacggtgc agacggcata cggttttatc
    10081 agagcagagt ccttacatcg gcagcaatcg tttccggctc gcttccgtaa ggcgagaaaa
    10141 tggcaacctc accgtttttg tcgatgagat acgcaccgga agagtggtcg accaaatagt
    10201 tttcgctgtc gtctttttga ttgactttgg cagaaaccac gcggtattgc tgcttgatga
    10261 ccggcaggtt ttggtcgccc gttgccgtca gaccgataaa gtccggattg aactgtttgg
    10321 catacttgcc gatgatttca ggcgtgtcgc gttccggatc gatgctgacg aacaccactt
    10381 tcacgtcctt agcctgcccg cccaactgct tcaacgtgtc gctgtacgtc aaaagctctg
    10441 tcgggcagac atcgggacag tgcgtaaagc cgaaagacag aatcacgacc ttgcctttca
    10501 aatcgctcag gctgaaaggc ttgccttcgc cgtcggtcag cgtgaaatcg ccgccgatgt
    10561 cttccttacg catatccgta ccgcgcgttt gcggctttgc cgcattttcc gcagctgacg
    10621 cggatgcact tgaagaagcg gcttgcgccg cactgttgtc ttgaggtttg caggcggcaa
    10681 gcgcggcaag tacgaaaacg cccggcaaaa aggaacgcgg tacggaaaac atgacaatct
    10741 cccaagaata aatagcaaaa cctgattcgt acggaatttg aatatttcca tcttaacgcg
    10801 tttggcagat ttaatgtagt atttttttac gttgtttgca cggcaggaat tgccgaatca
    10861 tacagaatct ttggagcgga aagaacagta agattaaaat aataaaaatt ctatttaaaa
    10921 tcaaaaataa aataaaaaca gataaaataa tttcaaaaaa aaggtttaca gaatttcaaa
    10981 agccattata atgacgactt cttcaccagc ccaggtggcg aaattggtag acgcagggga
    11041 ctcaaaatcc cccgccgcaa ggtgtgtcgg ttcgagtccg accctgggca ccacaaccgc
    11101 ttttagagcg gttatttttt tgtctattgc tgtttaatga aatagataaa actaa
//
LOCUS       DAXHMX010000062        11065 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHMX010000062 DAXHMX010000000
VERSION     DAXHMX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.062"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(60..1616)
                     /gene="dnaA"
                     /locus_tag="ACFM4S_001170"
     CDS             complement(60..1616)
                     /gene="dnaA"
                     /locus_tag="ACFM4S_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6050852.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1909..2043
                     /gene="rpmH"
                     /locus_tag="ACFM4S_001171"
     CDS             1909..2043
                     /gene="rpmH"
                     /locus_tag="ACFM4S_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6050853.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2046..2411
                     /gene="rnpA"
                     /locus_tag="ACFM4S_001172"
     CDS             2046..2411
                     /gene="rnpA"
                     /locus_tag="ACFM4S_001172"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6050854.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2477..2698
                     /gene="yidD"
                     /locus_tag="ACFM4S_001173"
     CDS             2477..2698
                     /gene="yidD"
                     /locus_tag="ACFM4S_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6050855.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            2870..4507
                     /gene="yidC"
                     /locus_tag="ACFM4S_001174"
     CDS             2870..4507
                     /gene="yidC"
                     /locus_tag="ACFM4S_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6050856.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4588..5313)
                     /locus_tag="ACFM4S_001175"
     CDS             complement(4588..5313)
                     /locus_tag="ACFM4S_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6050857.1"
                     /translation="MSEGMMSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAE
                     KTARAHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVA
                     DPGANLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLK
                     ALEQRSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVV
                     QWRKRKEMPNLKKRPTIFVMYAG"
     gene            complement(5360..5950)
                     /locus_tag="ACFM4S_001176"
     CDS             complement(5360..5950)
                     /locus_tag="ACFM4S_001176"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC6050858.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPKALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            6098..6601
                     /locus_tag="ACFM4S_001177"
     CDS             6098..6601
                     /locus_tag="ACFM4S_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6050859.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            6635..6814
                     /gene="rpmF"
                     /locus_tag="ACFM4S_001178"
     CDS             6635..6814
                     /gene="rpmF"
                     /locus_tag="ACFM4S_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6050860.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            6989..7516
                     /locus_tag="ACFM4S_001179"
     CDS             6989..7516
                     /locus_tag="ACFM4S_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6050861.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7604..8659
                     /gene="plsX"
                     /locus_tag="ACFM4S_001180"
     CDS             7604..8659
                     /gene="plsX"
                     /locus_tag="ACFM4S_001180"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6050862.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            8795..9046
                     /locus_tag="ACFM4S_001181"
     CDS             8795..9046
                     /locus_tag="ACFM4S_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6050863.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            9073..9489
                     /locus_tag="ACFM4S_001182"
     CDS             9073..9489
                     /locus_tag="ACFM4S_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050864.1"
                     /translation="MKTSTVVFGGFFMADNRERIQIPVLENPGIREINHFFSVSNFEK
                     KAGVLVFRIIPEPKFGNTELTIYFEEGCYLPMIQTILEDGDIEVKNLKTENYSGNTME
                     IWGDFYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            9509..9712
                     /locus_tag="ACFM4S_001183"
     CDS             9509..9712
                     /locus_tag="ACFM4S_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050865.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPIHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            9739..10701
                     /locus_tag="ACFM4S_001184"
     CDS             9739..10701
                     /locus_tag="ACFM4S_001184"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6050866.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            10748..>11065
                     /locus_tag="ACFM4S_001185"
     CDS             10748..>11065
                     /locus_tag="ACFM4S_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6050867.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         2855 a         3286 c         2560 g         2364 t
ORIGIN      
        1 ctatatcttt acccttcgga cggcatgggc aatatcatgt cgtctgaaaa cgttttccat
       61 cagttttgaa tcagaatcag cagcttttca taatcctgag ccaattccgg atcttcttcg
      121 cgcagtttcg ccactgccct gatgccgtgc ataacggtcg tatggtcgcg tccgccgaac
      181 gaatcgccga tagacggcag gctcaaagtg gtcagttctt tggtcaggct catcgccacc
      241 tggcgcggac gggcaatgtt tcgtgtccgt ttcttaccga gcacatcgct gattttgatg
      301 cggtaatatt tcgcaacggc atcaataata atatcggcgg tgatgacttt gtgcttctcg
      361 gcaataatgt cctgcaaagc agtacgcgcc aaatcgatgt cgatgacggg acggttcata
      421 aagcggctgc tcgctccgac gcggttgaac gcgccttcaa gctcacgcac gttggaacgg
      481 atcagattgg caatgaacag cgcggcttcg tcttcaatac tgatgcccgc cgcttccgcc
      541 tttttctgca aaatggcgat acgcatttcc aattcgggcg gctcgagttc caaagtcagt
      601 ccccatgaaa aacgggattt gaggcggtcg tccatacctt cgattttggc gggtaaaaca
      661 tcgcaagtga ggatgagctg ttttttctcg ttatggaaat gattgtacag atagaagaat
      721 tcctccatcg tacggtcttt gcctttgatg aactggatgt cgtcgataat cagcaggtcg
      781 tattgcttgt attgctgctt gaacacgtcg taggtattgt tgcgaaccgc cttcataaag
      841 ctgcggatat agtcgtccga atgcatatag cgcactttgg catcgggacg gtttttcaaa
      901 agctcgttgc cgaccgcctg taccaggtgg gttttgccca aacccgtgct gccgtacagg
      961 aaaaacgggt tgtagctctg ccccgggctt tccgcaatcg cctgcgccgc agccgccgca
     1021 aggcggttgc ccttgccttc gaccaatgtg tcgaacgtat agtccggcga cagattggtc
     1081 tgctcgtaac gtgcctcttc cgcatcgcgc tgcgcatccg tccgcgcttt cgcaagcacc
     1141 tccgattccg gacgggaagc aggttcggca gtatggcgcg gctcatgcgg gaggtttttc
     1201 atacgttccg ccagaatatc cgccgccgtt ttcgacgcag cgggtttgac aggctcttca
     1261 gacggcagct cgtccaacag aacctcctgc acaggcatct cctccgaccc cgcgtgcaag
     1321 gacggctcgg caggttcgac agcaccttca accgccgcca tctcataacg cacgccttct
     1381 cctggtttga atacgaaggc ggaacggcca gcagccaact cttccctcac cgcttctatt
     1441 ttcccggcaa actggctctt aagcatattg caggcaaact ggttcttgcc gtacaccacc
     1501 catacgccac cctcctcacc gacggtaagg ggcgcaatcc attgcgcaaa ctgcccttga
     1561 ggcaacatat cgtgaagacg gcggaggcac agcggccaaa actctgctaa tgtcatggat
     1621 aagctcgaat cggtaaaaat gaaatcgaaa acaaagaaaa tataatattt tcaaaaagaa
     1681 aacaaatctg ttgaacacac atcggttcaa aatgcagcga accgaattat accgactcgc
     1741 cgatatttta tccacaaccc gtgcaaaatt tatccacaga aaggcggcag aaatccgcag
     1801 gcaatcgggc aatcctcctg caaagtttct atattgattg acaaaagcgg caaattggag
     1861 tgtaattcac ggtttaatta tctacccatt ctatttttag gaaacatcat gaaacgcact
     1921 tatcaacctt ccgttaccaa acgcaaacgc acccacggct tcctggtgcg ctccaaaaca
     1981 cgcggcggcc gcgcagtatt ggctgcacgc cgcgccaaag gccgcaaacg cctggcggta
     2041 taattttgga ctaccgcttc ggaaggcagt accgcttgtt gaaaacggat gatttttcat
     2101 ccgtttttgc attcagaaac cgccgcagcc gcgacctgct gcaagtttcg cgttcaaacg
     2161 gcaacgggct ggaccatccc cgcatcggtc tggtggtcgg caaaaaaacc gccaaacgcg
     2221 ccaacgaacg aaattatatg aagcgcgtca tccgcgactg gttcagattg aacaaaaacc
     2281 ggctgccgcc gcaggatttc gtcgtgcgcg tccgccgtaa attcgacagg gctaccgcaa
     2341 aacaggcaag ggcggaactg gcacaactca tgttcggcaa ccccgcaacc ggatgcagga
     2401 aacaggcatg atcagaacgg tactctgcag gcaaggttca gacggcaacg ggtttcccat
     2461 actaaggaac atcccgatga acttcctatt gtccaaactc ctgctgggac tgatacggtt
     2521 ctaccaatat tgcatcagcc cgctgattcc gccgcgctgc cgttatacgc cgacctgttc
     2581 gcaatacgcg gtcgaagcgg tcaaaaaata cggcgcattc aaaggcggcc ggctcgccat
     2641 caagcgcatt gcacgctgcc accctttcgg cggacacgga cacgaccccg ttccctgacc
     2701 cgacgcaata ttcaaattgc acgctttcct tttatttccc atcggtttct atataatgcc
     2761 gtctgaactt tcgggctggc ggcacgaccg tcgggtatga agcccgccct tattccccgt
     2821 ctatcggaac acgcaacctg cggcatttcc gaccattcag gaaactctta tggattttaa
     2881 aagactcacg gcgtttttcg ccatcgcact ggtgattatg atcggatggg aaaagatgtt
     2941 ccccactccg aagcccgtcc ccgcgcccca acagacggca caacaacagg ccgtaaccgc
     3001 ttccgccgaa gccgcgctcg cgcccgcaac gccgattacc gtaacgaccg acacggttca
     3061 agccgtcatt gatgaaaaaa gcggcgacct gcgccggctg accctgctca aatacaaagc
     3121 aaccggcgac gaaaataaac cgttcatcct gtttggcgac ggcaaagaat acacctacgt
     3181 cgcccaatcc gaacttttgg acgcgcaggg caacaacatt ctaaaaggca tcggctttag
     3241 cgcaccgaaa aaacagtaca gcttggaagg cgacaaagtt gaagtccgcc tgagcgcacc
     3301 tgaaacacgc ggtctgaaaa tcgacaaagt ttatactttc accaaaggca gctatctggt
     3361 caacgtccgc ttcgacatcg ccaacggcag cggtcaaacc gccaacctga gcgcggacta
     3421 ccgcatcgtc cgcgaccaca gcgaacccga gggtcaaggc tactttaccc actcttacgt
     3481 cggccctgtt gtttataccc ctgaaggcaa cttccaaaaa gtcagcttct ccgacttgga
     3541 cgacgatgcc aaatccggca aatccgaggc cgaatacatc cgcaaaaccc cgaccggctg
     3601 gctcggcatg attgaacacc acttcatgtc cacctggatc ctccaaccca aaggcggaca
     3661 aagcgtttgc gccgctggcg actgccgtat cgacatcaaa cgccgcaacg acaagctgta
     3721 cagcaccagc gtcagcgtgc ctttagccgc tatccaaaac ggtgcgaaat ccgaagcctc
     3781 catcaacctc tacgccggcc cacagaccac atccgttatc gcaaacatcg ccgacaacct
     3841 gcaactggcc aaagactacg gcaaagtaca ctggttcgcc tcccccctct tttggctttt
     3901 gaaccaactg cacaacatca tcggcaactg gggctgggcg attatcgttt taaccatcat
     3961 cgtcaaagcc gtactgtatc cattgaccaa cgcctcttac cgttcgatgg cgaaaatgcg
     4021 tgccgccgcg cccaaactgc aagccatcaa agagaaatac ggcgacgacc gtatggcgca
     4081 gcaacaagcc atgatgcagc tttacacaga cgagaaaatc aacccgctgg gcggctgcct
     4141 gcctatgctg ttgcaaatcc ccgtcttcat cggattgtat tgggcattgt tcgcctccgt
     4201 agaattgcgc caggcacctt ggctgggttg gattaccgac ctcagccgcg ccgaccccta
     4261 ctacatcctg cccatcatta tggcggcaac gatgttcgcc caaacctatc tgaacccgcc
     4321 gccgaccgac ccgatgcagg cgaaaatgat gaaaatcatg cctttggttt tctccgtcat
     4381 gttcttcttc ttccctgccg gtctggtatt gtactgggtg gtcaacaacc tcctgaccat
     4441 cgcccagcaa tggcacatca accgcagcat cgaaaaacaa cgcgcccaag gcgaagtcgt
     4501 ttcctaaatg ccgcagcatg aaaaatgccg tctgaaacct gttcagacgg catttttatt
     4561 gttcaccccc tatcggggcg gaaatcttca acctgcatac atcacaaaaa tcgtcgggcg
     4621 ttttttcaga ttgggcattt cttttctttt tcgccactgc acaaccgtct ggctgatgat
     4681 ttcctgagtc ggcaacgtca aatccgtagc cgtacacaaa cgcgtttcag aatgcaggtt
     4741 ttccaccgca tccgccaaca acacatcgtt gcgatacggc gtttcgataa acagctgcgt
     4801 ttcattctgc tggcgcgaac gttgctccaa agccttcaaa ctctgaatcc gctcgttttt
     4861 ttcagacggc agataaccct taaacgcaaa actctgcccg ttcgcacccg aagccatcaa
     4921 agccagcagc aggctggaag gcccgaccag cggacgcact tcaaaaccgt gtttatgcgc
     4981 caaagccacc agattcgcac ccggatcggc aacagccggg caacccgcct cgctgacaat
     5041 gcccatactg cgcccttctt gcaaaggttt cagcaattcc agcaaagtct tcaaatccgt
     5101 gtgttcgttc agcgtttgca aattcagttc gcggataggc attgtcacgc ccaaatgttt
     5161 caaatgcgcg cgcgccgttt tttccgcttc cacgacaaaa tccgtcagcc cgacaatcgc
     5221 ctgttgttca tgcggcaaca ggcacggcgt atcgggcgta cccaaaggcg taggaatcaa
     5281 atacaaaaca ggagacatca ttccctcact catcggttaa aaatgccgtc tgaacccttc
     5341 agacggcata aacgggcagt tacaaaacat ccacgccctc atttttcaaa aaatcgacca
     5401 gacggaaaac cggcaaaccg attaaagcat tcggatcggt actctcaatc ctttcaatca
     5461 acaatgcacc caaatcctca ctcttcagcg cacacgaaca ataaaccgca tcaggctcgc
     5521 gctccaaata gcggaggatg tgcaactcgt ccaactgcct catcacgacc accgtcttat
     5581 cgatatgccg ctgcatcctg cccgtaaccg tatttagcag aacgaccgcg ctgtaaaact
     5641 caatctccct gccgctcaaa tgcatcaaca tcttttgcgc attagcaagg ttcatcggct
     5701 tgccccactg cctgccgtcg caccacgcca cctggtccgc accgacaatc aacgcctttg
     5761 ggaaacgccc ggtcaacgac cgcgccttac cctcggcaag gcgcaatgcc gtctgagggg
     5821 cggattcccc caacatcggc gtttcgtcaa aatcggggga cgccgcctga aaggcaatgc
     5881 cgagcctttc catctgttcg cggcggaaaa ccgaactcgt acccaaaatc aaaggcagtt
     5941 ccaaacccat cccatcctcc ttaccgttga aaacacgccc gaaggggcgg taaaatccag
     6001 ccatgcgccg aaacacggat acccgccttc ggcgtaccgc aacatttttc ttaaaaatat
     6061 tgacgttaga acatctaaat tatatcatac cccgtttatg tcagacccta atttgattga
     6121 cccggaaatt ttcgccgccg aagggcagaa tctgcaaggc agttttctgc tggaagaatt
     6181 ggatgaacgc gtcagttcgc acgattatcc cgccgacagg cagaccaaaa tatcgtttac
     6241 actgaccggc ggtcgcgacc ggctgcaacg cctgttcctc gacctgaacg tcaaagccga
     6301 tatgcccctg atttgccaga gatgtatcaa acccatgccg ttcatgcttg atgaaagcag
     6361 ccgtatcgtc ctgttttcca acgaagagtc cttggacgaa tccatgcttg ccgacgaaga
     6421 actcgaaggc atactgattg aaaaagaact cgacgtgcgc acattggtag aagaccaaat
     6481 cctgatgtcc ctgccctttt cgccgcgaca cgaagactgc ggcgacaatg ggacactgga
     6541 agaagtcaat cgggacaaac ccaacccctt tgctgttttg gcaggtttga aaagcaattg
     6601 attaggacac agtttattta tctaggagct tgaaatggcc gttcaacaaa acaaaaaatc
     6661 cccttccaaa cgcggtatgc accgttcgca cgacgcgctg accgcgcctg cactgtctgt
     6721 cgacagcaca accggcgaag tacaccgccc gcaccacatc tcccccaacg gtatgtaccg
     6781 cggccgcaaa gtggtcaaag ccaaaggcga ataatcccta ttcgactgac tgaaaaagcc
     6841 agaacattgc catgcaatta ctggcttttt ttgcattgga cgcaccatcc gtccaaactt
     6901 tcgccatacg tcaacacaca ggggcaaagc gttccgtata ataccccgtg aaaatattcc
     6961 aaaagcccca accaccaagg aaattccgat gaaacagaaa atctggtaca cctacgatga
     7021 catccaccgc gtcatcaaag cattggcaga aaaaatccgg aacgccgaca tcaaatacga
     7081 tgccatgatt gccatcggcg gcggcggctt tattccggca cgtatgctgc gctgttttct
     7141 ggaaattccg atttatgccg taaccaccgc ctattacgac agcgacaacg aaggacaggt
     7201 tacagaagaa gtcaaaaaag tccaatggct cgaccccgtt cccgaagccc tgcggggcaa
     7261 aaacgtcctt gttgtcgatg aagtggacga cagccgcgta accatggaat tttgcctgac
     7321 agagctgctc aaagaagact tcggtacgat cggcgtcgcc gtactgcacg aaaaaatcaa
     7381 agccaaagca ggcaaaatcc ccgaaggcat tccctatttc agcggcatca ccgtagaaga
     7441 ctggtggatc aactatccgt gggacgcact cgacatcgac gaacacaacc gccttgccga
     7501 ggccggccga ggctgaccct ttcagacggc atattttccg aaccgatgcc gtctgaagcc
     7561 cgcacgaccc ctgccgcaga ccgaaaacct accggagaac cctatgatta cacttgccgt
     7621 agatgccatg ggcggcgacc aaggacttgc cgttaccgta cccggcgcaa ccgcattcct
     7681 ccaagcacac cccgatgtcc gcctgattat gaccggcgac gaaacgcaac tgcgccaagc
     7741 cctgaccgcg gcaggcgcac cgatggaacg catcgacatc tgccatacca cccaagttgt
     7801 cggcatggat gaatcccctc agtccgccct aaagaacaaa aaagactcct ccatgcgcgt
     7861 tgccatcaac caagttaaag aaggcaaagc ccaagccgcc gtatccgcag gcaacacggg
     7921 cgcactcatg gcaaccgcac gtttcgtcct caagaccata cccggtatcg aacgccccgc
     7981 catcgccaaa ttcctcccgt ccgacaccga ccatgttacc ctcgccctcg accttggcgc
     8041 gaacgtcgac tgcacgcccg aacagctcgc ccaatttgcc gttatcggca gcgaactcgt
     8101 ccacgcactc catcctcaaa aaggacagcc gcgcgtcggg ctggtcaacg tcggcacgga
     8161 agacatcaaa ggtacggaca ccgtcaaaca aacctacaaa ctgctgcaaa acagcaaact
     8221 caactttatc ggcaacatcg aaagcaacgg catcctgtac ggcgaagcag atgtcgtcgt
     8281 cgccgacggc tttgtcggca acgtcatgct caaaaccatc gaaggcgcgg tcaaattcat
     8341 gagcggagcc atccgccgcg aattccaaag caacctgttc aacaaacttg ccgccgttgc
     8401 cgccctaccc gccctcaaag ggctgaaaaa caaactcgat ccgcgcaaat tcaacggggc
     8461 catcctgctc gggctgcgcg gcatcgtcat caaaagccac ggcggcacag acgaaaccgg
     8521 tttccgctac gcactcgaag aagcctacca cgaagccaag tccgccggcc tttccaaaat
     8581 cgaacaaggc gttgccgaac aactcgccgc actcgaaact gccaaagccg tccaaaacga
     8641 aaatgtcggc ggtctgtaac acacacgatg ccgtctgaac gcccccgccc ctttcagacg
     8701 gcatccgccc gccccgaacc tgcgggcgcg gacggcgatg cacctgtccg gcacttccca
     8761 aatacttccc tgtaaaataa ggaatatttg aaaaatgaag acattagaaa aacggatgaa
     8821 agctctagac aaacggatga tgaagttcgg aaaatccctt gaaggcaggc ttgatgcccg
     8881 tctgattgaa tccgcattgg attatattca ttattcggaa cgttttttgg cttttgaaat
     8941 cctgtgtact tatatcgaag atttcgatgt ccggctgacg gaacaagaat cccgggaaat
     9001 ttcttttatc aacaaggaat ttgagataga aagcacgtcc gattaaccaa taaagccaat
     9061 gatttgataa gaatgaaaac atcgacggtc gtttttggcg gattttttat ggcagacaac
     9121 agggagcgaa tccaaatccc cgttttggaa aatcctggca ttagggaaat caatcacttt
     9181 ttttccgtat caaattttga gaaaaaagcc ggcgtccttg ttttcagaat tattcctgaa
     9241 cccaaatttg gcaataccga attaactatc tattttgaag aaggatgtta tttgccaatg
     9301 attcaaacaa ttttagaaga tggggatatt gaagtcaaaa accttaaaac agaaaattat
     9361 agtggaaata caatggaaat ttggggagac ttctacccta ttgagcatat ctctaaaaat
     9421 atttctacaa ttcaaaatat aatttctgaa tttatgatac aaaaacaaac accggcacca
     9481 ataatttgat ggaaaaccaa caatcaaaat gccgtctgaa gcatgtttgg ttttcagacg
     9541 gcatttcctt cgcttaaaac agcgtatcgg caaccccgcc ctgcctatcc acggcaatct
     9601 gcatctgaaa accatctgta tcccaaacca cacccccatc cctgtttccg tcatttgcac
     9661 cctgtccgta ttgggcaatc atcggttttt cgcttacaat agccgaatct gaaccaactc
     9721 tctaaaaagg ccgttcccat gcagtatgca aaaatttccg gcacgggcag ctatcttcct
     9781 gccaaccgcg tcagcaatga cgaccttgcc caaaaggtag atacctctga cgagtggatt
     9841 accgcgcgca cgggcatcaa gttccgccat attgcagccg aaaatgagaa aaccagcgat
     9901 cttgccgccg aagcagcccg ccgcgcgctg gatgcagccg gattagacag cggcgaaatc
     9961 gatttgatta tcgtggcgac ggcaacgccg gatatgcagt ttccttctac cgccaccatc
    10021 gtgcagcaaa aattgggcat caccaacggc tgtcccgcgt ttgacgtaca ggcggtgtgc
    10081 gccggcttta tgtacgcgct gaccacggca aacgcctaca ttaaaagtgg catggcgaaa
    10141 aacgcgctgg tcatcggcgc ggaaaccttc agccgcatcg tcgattggaa cgaccgcaca
    10201 acctgcgtat tgttcggcga cggcgcgggc gcggtggttt taagcgcgtc ggacacgccg
    10261 ggcatcatcc acagcaaact caaggccgac ggcaattatc tgaaactctt aaacgtcccc
    10321 gggcaaatcg cctgcggcaa agtttccggt tcgccgtaca tttcgatgga cggtcccggc
    10381 gtgttcaagt ttgccgtcaa aatgctgtcc aaaatcgccg atgacgttat cgaagaagcc
    10441 ggttataccg ccgctcaaat cgactggatc gtgccgcatc aggcaaaccg ccgcattatc
    10501 gaatcgaccg cgaaacattt aggtttgagt atggacaaag tcgtcctgac cgtccaagac
    10561 cacggcaaca catccgccgc atcgattccg ctggctttgg atacgggcat ccgcagcgga
    10621 caaatcaaac gcggtcaaaa cctgctgctc gaaggcatcg gcggcggttt cgcgtggggc
    10681 gcggtgctgt tgcaatattg aacccgatgc cgtctgaaac aggctttcag acggcatttc
    10741 ccatatcatg aagcggcagg ctttcttcaa actgatggcg tgtgcggcat ttctgtctgc
    10801 cgtttcgctg cgcctccccg tattgggcgc gtgttacgca atattgtccc tctatgcgtt
    10861 tgcactttac ggcatcgaca aacggcgtgc cgtgcgggga aaacgccgca ttcccgaaca
    10921 ccgcctgctg ctgcctgcct tgttcggcgg ttgggcgggc gcatacttgg gcagcaggat
    10981 attcaggcat aaaacggcga aaaagcgttt tgttgtgctg ttccgtctga ctgtttcggg
    11041 caatgtcctg gcgaccctca tcctg
//
LOCUS       DAXHMX010000063        11045 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHMX010000063 DAXHMX010000000
VERSION     DAXHMX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11045
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.063"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            142..1539
                     /gene="aspA"
                     /locus_tag="ACFM4S_001186"
     CDS             142..1539
                     /gene="aspA"
                     /locus_tag="ACFM4S_001186"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC6050868.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1611..2486)
                     /locus_tag="ACFM4S_001187"
     CDS             complement(1611..2486)
                     /locus_tag="ACFM4S_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6050869.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDIGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVEAAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2488..3222)
                     /locus_tag="ACFM4S_001188"
     CDS             complement(2488..3222)
                     /locus_tag="ACFM4S_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC6050870.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYM
                     PIVGVILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATN
                     VWYFYRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3245..3724)
                     /gene="ybaK"
                     /locus_tag="ACFM4S_001189"
     CDS             complement(3245..3724)
                     /gene="ybaK"
                     /locus_tag="ACFM4S_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6050871.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3799..4161
                     /locus_tag="ACFM4S_001190"
     CDS             3799..4161
                     /locus_tag="ACFM4S_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6050872.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATA"
     gene            4196..5059
                     /locus_tag="ACFM4S_001191"
     CDS             4196..5059
                     /locus_tag="ACFM4S_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6050873.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPKNTLPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELAAPNGGIFKA
                     VHLFFKTENAE"
     gene            5153..6112
                     /gene="ttcA"
                     /locus_tag="ACFM4S_001192"
     CDS             5153..6112
                     /gene="ttcA"
                     /locus_tag="ACFM4S_001192"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6050874.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6206..9145)
                     /locus_tag="ACFM4S_001193"
     CDS             complement(6206..9145)
                     /locus_tag="ACFM4S_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC6050875.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYADNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTRQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFAKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAKIHPAVSDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLGLLNKLFSDGIIDDNENFTE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYRELLEKLAQTAKIRMQTLHQAFYRVRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYQLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTQSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9158..>11045)
                     /locus_tag="ACFM4S_001194"
     CDS             complement(9158..>11045)
                     /locus_tag="ACFM4S_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6050876.1"
                     /translation="PDLIDTAGVNTEAIYTSDGITANSTQLEQLKKLFPACFDADGNF
                     LIDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQ
                     NLLIRGDNLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANI
                     DEDEAARILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLL
                     CDEVFGEGNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKK
                     IVINDELYWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSY
                     GKTIIGKLRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPL
                     DLLKRLVLSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTA
                     EKSEARKAGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDF
                     SPEQPDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMN
                     AGFTSADLLAFIRKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNV
                     IIRV"
BASE COUNT         2658 a         2950 c         2603 g         2834 t
ORIGIN      
        1 cccgaaaaca acgtttcccg ttttcagacg gcatacgata tggacaattt catccggccg
       61 gtatagtgtt catcctgcgg cggcatcttt ccgatggcgc attttttcaa tattaattat
      121 ccgacttaca aaggtaaaaa catgactgtc cgtatcgaac acgatttatt gggcgaccgc
      181 gagattcccg ccgaagtgta ttggggcatc cacaccctgc gcgccattga aaactttaaa
      241 atctccacac aaaaaatttc tgacgtgccg cagtttgtcc gcagtatggt gatggtgaaa
      301 aaggcgacgg cacaggcaaa cggtgagttg ggcgcggtca agcccgaaat cgccgccgcc
      361 attgaaaaag cctgcgacga agttctgctg aacaaccgct gcctcgacca attcccgtcc
      421 gacgtgtatc agggcggtgc gggtacttcg gtcaatatga acaccaacga agtcatcgcc
      481 aacctcgcat tggaagtctt gggatacgaa aaaggccgct acgacatcgt caatccgatg
      541 gatcacgtca atgccagcca atcgaccaac gatgcctatc ccacgggctt ccgccttgcc
      601 gtgtattaca gcatcggcga attgctcgac aaactgactg tcctgaaaaa cgcctttgcc
      661 gccaaagccg aagaatttaa agacgttttg aaaatgggcc gcacccagct tcaagatgcc
      721 gtgccgatga ctgccggcca agaattccaa tctttccaag tgttgttgga agaagaaatc
      781 ctcaacctcg accgcacccg ccaactgctg ttggaagtca atttgggcgc aacggcaatc
      841 ggcacgggcg tgaacacgcc caaaggctac gccgaactgg tggtcaaaaa actctccgaa
      901 gtcagcggct tgccttgcaa actgaccgaa aacctgattg aggcgacctc cgactgcggc
      961 gcatatgtga tggtacacgg cgcattgaag cgcacggcgg tcaaactctc caaaatctgc
     1021 aacgacttgc gcctgctctc ttccggcccg cgtgccggct tgaaagaaat caacctgccc
     1081 gaattgcagg ccggttcttc cataatgccc gccaaagtca atcccgtgat tcccgaagtg
     1141 gtcaaccaag tctgcttcaa agtcatcggc aacgacacga cgattacctt cgccgccgaa
     1201 gccgggcaac tgcaattaaa cgtgatggag ccggtcatcg cccaatgtat gtttgaaacc
     1261 atttccctct tgggcaatgc cgcagtcaac ctgtccgaca aatgcgtcaa aggcattacg
     1321 gtcaaccgcg aaatctgcga acgttacgtt ttcaactcca tcggtttggt gacttatctg
     1381 aatccgtata tcggacacca caacggcgat ttggtcggca aaatctgcgc ccaaaccggc
     1441 aaaggcgtgc gcgaggttgt cttggaacgc gggctgttga gcgaagaaga actcaaccgc
     1501 atcctctctc ccgagaacct gatgaacccc catctgtaac gttgcagaca aatgccgtct
     1561 gaagcaggga tgctgcttca gacggcattt tgttatatca atcagccgga ttatatgccg
     1621 aacagtacct tgacttcccc ccaataaaac aggcaggcga tagaacacag tgccaacacg
     1681 attcccgccg tattgattgt gttgattttt tccttaaaga acaatgcgcc cgaaagcgta
     1741 cccaaaacaa tcacgccgat attcataccg gcaaaaacca aggtcggatt atccttcatc
     1801 atttggtgcg cggtgatgta ggttacgata ttcataaaat tcaaaccgcc caagaatatg
     1861 ccgccgacca cactctcaac acgccatctg accgatttgg caaacaggca ggcaaacatc
     1921 agcacacccg ccagcacaaa tgcaaccagc aggttgcccg caaatgccgt tccgcttttg
     1981 gcaagctgtt tgaacaggat atcgataatg ccgtaacctg cccacacgcc cagcagcaat
     2041 gccgcctgcc gccacgcgct tcctgatttt ttgccaccgc tgtgtttcca aagcaggcag
     2101 aacagtgcgg caaatgcgag gcacagcccg attagtttgc cttcgctgag tttttcgcca
     2161 aacagcgtca aggcggcaac aatcggcaaa aacagcgaca aacgctgcgc cgcgtcggat
     2221 ttgacgatac cggcggcttc cacagatttg cccattatca cgaatacgga cggcagcagc
     2281 acgcccaaag cggcaaacag cggccacgtc ggcaaaaatg cgccgatatt gccgatatcc
     2341 ggcttcaata ccagcagggt cagtatgacc gcgaccacat aattgacggc gaccgcctgc
     2401 gcgatgtcga tttttttctt ccttgccatt ttcagcaaaa tggaaaccga cacgctgaac
     2461 acgatgctga ttaacaggta agccatacta tatccatttc tcgggtgcaa ccgccctgcc
     2521 gccgttgaag cggataaagc cggcgatgat gcggtagaaa taccagacgt tcgttgcaat
     2581 aataatcagc acgccgatac ccaaaagggc agtcagcgca cccaaaatat aaagccaaaa
     2641 tgtgcgccaa aaggttttaa tcaggtattc ggtatgtgcg gcatagacaa tgctgtccaa
     2701 actgttccgt ttcatatagg cgagaatcac gccgactatg ggcatatagg cgaaaatcac
     2761 gccgactatg ggcatgaaca gcatgattgc acccaaaccg aacaggatgt aggcggcgag
     2821 gacatagttg cgcccgcttc cgccgccgga cggttcgtaa gtatggcacg aacgtgatgc
     2881 ctgccgttca aacgcctgtc tgaacgcctg tctgaacgcc tgttcgcggc gcatctgttc
     2941 gcggtaaaac tgctccctgc gcgcctgttc gcggcggaat gcctcttcct gccgcccgcg
     3001 ttcctcatgc cgtctgaagg acgcgtcgta ttgcatccgt ttcgacaggt cggaaagcgt
     3061 atcgtaagcc cgttggattt ctttgaactt ttcttccgcc ttcggattgc ccggattgcg
     3121 gtcgggatga tatttcatcg ccagcttgcg gtaggcgcgt ttgatttcgt ccgctcccgc
     3181 ctgcggcgac acgcccaata cggcatacaa atctttgtcc atattgttcc tttatacttt
     3241 cccgtcaaac cgccgcctgt attgttttcg ggttcaaaat atttaaatct ccgggacgga
     3301 tgccgataat gaacccgcgt tttccgccgt tgatatagat ggtttccaaa tccatcaccg
     3361 actgttcgac gtaaatatcc aactttgtcc ggatgccgaa cggcgttgtg ccgccgacca
     3421 gatagcccgt ccacttgttt gcctgtacgg gcgtggcggg ttcgatgtgt ttcgcaccca
     3481 aatgccgcgc cagattgcgg gttgaaatct gcttgtcgcc gtgcatcaag acaatcagcc
     3541 ccttaccgtt ttcatcttgc aaaacaatgg ttttaatgac caagtgttcg tctttgccga
     3601 atagtcgggc aaactgcgcc gtgccgccgt gttcctcata ggcataggta aaaggttcaa
     3661 attcgatgcc gttttcacgc aaaacgcgca cggcaggtgt tatcggataa gtgtgtttac
     3721 tcatttttcg tcatgcaaaa catggaaatc ggtcaagcgc aatgttacca tatatcccct
     3781 ttatcgaagg aaaccgatat ggatatccga tatttcggca caacgccccg ctattccgaa
     3841 gccgtcggcg caaacggctt gattttcctc tccggcatgg ttcccgaaaa cggtgaaacg
     3901 gctgccgaac agactgccga cgtgcttgcc caaatcgacc gctggctggc ggaatgcggc
     3961 tcggacaaag cgcacgtttt ggatgcggtc atctatctgc gcgatatggg cgactatgcg
     4021 gaaatgaacg gcgtgtggga cgcttgggtt gccgccggca gaacgcccgc ccgcgcctgc
     4081 gtggaagcgc ggctcgcccg tccggagtgg cgcgtcgaaa tcaaaataac cgccgtgaag
     4141 aaagatgcgg caaccgcata aacagatttc ctttatattt cagagtacgc aaatgatgaa
     4201 aaccttactg ccctttttcg gcgtggtacg cgtcagcggc gaagaccgcc aaaccttcct
     4261 gcacggacag ctttccaacg acatcaataa tcttcagacc gggcaggcgt gttatgcgac
     4321 ttacaacacg cccaaaggcc gtgtcatcgc caatatgatt gtcgtcaacc gaggcggcga
     4381 tttgctgctg attatggcgc aagacttgct tgaagcaacg gtcaaacgtt tgcggatgtt
     4441 tgtgttgcgt gccaaagccg tttttgaaat ccttgaagat tacgccgtcg gcgcggaatt
     4501 ggaagcatcc gccgaaccgc ttgccgcgca agaacccaac cttgcctttg ccgcacaaca
     4561 agattcagac ggcatctgct ctatcgctct gcctcatggc ggcatccttc gcatcgcccc
     4621 caaaaacacc ttgccgcctt atgatgccgc cgccgaaaac gcgtggcggc tgcacgaaat
     4681 ccgtagcggt tatccgtgga tatgcgcggc taccaaagaa actgccgttg cccaaatgct
     4741 caaccagcac atcatcggcg gcgtacactt caaaaaaggc tgctatcccg gtcaggaaat
     4801 cattgcccgt gcccagtatc gggggcaggt caaacgcggc ttggcggtat tgtcgggaaa
     4861 ttcggccgtc gaagcaggaa ccctgctgac ggcggacggc gaagaggccg gcatcgtact
     4921 cgacagcgtt caagattcgg aaaactttac cgcccttgcc gtgattaaat tctccgccgc
     4981 acaaaaagaa ctcgccgccc caaacggcgg tatcttcaaa gcggttcatc tcttctttaa
     5041 aaccgaaaat gcagaataat ccctctgatg ccgtctgaag cccttcagac ggcattgccg
     5101 ccccgtcaat cggttacaat gccgcctttc taattttcaa acccaaaccg ccatgtccaa
     5161 aaaaaccaaa caagaactcg aaaacaacaa actcagcaaa cgcctgcgcc acgccgtcgg
     5221 cgacgcgatt aacgatttca atatgatcga gccggacgac aaaatcatgg tctgcctctc
     5281 cggcggcaaa gacagctatg ccctgttaga catcctgcgc cagctccaag ccagcgcgcc
     5341 gattgatttc caactggttg ccgtcaatct cgaccaaaag cagccgggct tccccgaaga
     5401 agtattgccg acctatctcg aaagcatagg cgtgccgtac aaaatcgttg aagaagacac
     5461 ctactccacc gtcaaacgcg tattggacga aggcaaaacg acttgttcgc tgtgcagccg
     5521 cctgcgccgc ggcatcctct accgcactgc aaaagaattg ggctgcacca aaatcgcctt
     5581 gggacaccac cgcgacgaca tcctcgccac cttgttttta aatatgtttt acggcggcaa
     5641 actcaaagcc atgccgccca agctggtgag cgacaacggc gaacacatcg tcatccgtcc
     5701 gctggcgtat gtaaaagaaa aagacttaat caaatacgcc gaattaaaac aattccccat
     5761 catcccgtgc aacctctgcg gctcgcaacc caacctgcaa cgccaagtca tcggcgatat
     5821 gctgcgcgat tgggacaaac gcttccccgg ccgcatcgaa tcgatgttct ccgccctgca
     5881 aaacgtcgtc ccttcccacc ttgccgatac cgaactcttc gactttgtcg gattggaacg
     5941 cggtcaaacc ctgaaacacg gcggcgattt ggcgtttgac agcgaaaaaa tgcccgaacg
     6001 tttttcagac ggcagcgaag aagacgaaag cgaaatcaaa atcgagccgc aaaaagccga
     6061 acgcaaagtc atcaacattc tggcgaacaa gccgaaaacc tgcggcgcgt aaacctccac
     6121 cgtcaaaaat gccgtctgaa gcccgtttgg cgtttcagac ggcattttaa aatcccgaag
     6181 cagacggctt ccaaacattt gtttctcagt gtccgttttc agaatgcccg cctgcgggca
     6241 tattttgccc gatcagttcg gctatcctct cgccgtcaaa ctgcgttttg aacaccacct
     6301 tgatttcttt ggaaatctcg gcaaacagct tttcggcatg tttgattttc cgctcttcgc
     6361 tttttcgcaa atcttccgcc ccgtcagtcc ctttggcttc aatcacaaag ttcagaatct
     6421 cgccgctttg ggttttcacg atataggcaa aatcgggcga atacgtgccg ccgccggcaa
     6481 cggggatttt gatggagttt ctcggtattt tagtgaatac ggttacgcct tcaatttggt
     6541 tgttggcgac attttcatgt tctatatccg aatcgtagaa aatctcgtcg aagagatagc
     6601 cggcggcagg ccggtgctcc gtatcttcaa atcttcccaa atctgctttt ttcaccgcgc
     6661 gcggtttgcc gtctttatcg gtaaatttgg tcggatggat tttgctgccg acaagctggt
     6721 aatccagttc gaatttatgg aaggaatgat gaagcaaaaa ccggttgaag ccgtttttga
     6781 tttgggcgat ggtctgcata ttcaaaaaat cgccaatgtt cagttcgttg cggacgcggt
     6841 aaaacgcctg atgcaaagtc tgcatgcgga tttttgccgt ttgtgccagt ttttccagaa
     6901 gctcacggta agtcattgtg ttgaaacgga taaaatcttc atcttcaaag ctgtctatgc
     6961 ggcgggaaag cataagcccg ttgttgatat aagcttcgtt taccgccgtg cgtatgcctg
     7021 cctgcgggaa tttggcggcg ttttcacgca aataggcagt aaataaatcg acaaattcgg
     7081 cttcatcttt gattttgtac tgcaaaacgg ctttatggtg aatcagctcc cacaaggctt
     7141 tgagttcttc atatttgcct tcgcgcatga tgatggtgtc tttgccttcg tctttggcgt
     7201 tgctgacttt gcctttgtcc aaacctttgg ggaaggcttc gggataggcg gcttttaatt
     7261 tgtcatagcc gtcttcggta aagttttcat tgtcgtcaat gatgccgtct gaaaacagtt
     7321 tgtttaacaa tcccagaggt ttgatatcgg ggtatttttg caatattttt tgtttcagct
     7381 cttcggtaaa cttttttgag atttcttcct gaaaagaatt atcgttgatt tcgccgacaa
     7441 gctgcttcac aaagtctttt tcgctgctat cgacaaaata attcagtttg tacggcacat
     7501 cgcgcacccg cgccatcagc tcgtttaccg gcaggcgcag gccgcgtccg acttcttgca
     7561 gcttggaagt cgtgctgccg ctggaacgca gtttgcaaat ctggaaaacg ttgggattgt
     7621 cccagccttc gcgcagcgtc catttggaaa aaataaagcg gcgcgggttg tccaaagaca
     7681 acagtttttc cttatcgtgc aggatttcat tgatttcctg ctcgattcta tcgtcgctgt
     7741 ctgtattgtc tttggaaaaa taaccgccgt ggcaggcgga tacatcgtcc aacgtctttt
     7801 gcaggtaatc gcggtaaaac gggtcgcttt ccgttttcag gcggcgtgcc gcttccgcgt
     7861 gaatccagct ttcaaattta tctttcaggc tgcctgaaag ctcgttgccg ctgcggtagc
     7921 ccgcaatatc atcaataaaa aacagcgtca gcggcttgat tttgggctgt ggcgcgcgtt
     7981 ctgccaaaag tgcgcgttcc agcttgaaat gttcggcaac cgccctctgc atcatcgcat
     8041 cctgcaccgt ttgcgaatag gaataagggt tgatgacggc gcccgttttc aactccaagc
     8101 cgttgcttaa caccaccacg gttttattca ttttgtcgat tttcaaatct gaaacagcag
     8161 gatggatttt cgccaaatct tcgccttttg ccagtttgaa cgtctgcttt ttgtcctttt
     8221 cgtttaattc aaatttcgct tctttgccgc ccgacgacac cagttttacc gccgcatcca
     8281 tgccgccctg catttcttcc tgaaacacgc gcacgccttt gaccagcccg tcgttaaacg
     8341 cgtctactgc cgtcaaacgg taaagcaagt tgtaatattc atcgttaaat gtcgcaccgt
     8401 agcgcaaaat atattgcggt tttaagcgtt tgatattgcc ccatgttttc gcgctctccc
     8461 gggtcgggaa tttatgcggt tcgtccacaa tcacaaacgg gcgcacggcg gccaatgcat
     8521 caacgggatt gtcaaacaaa tctttcaatg ccttgtcgcc cgtatcgttc atggacgacg
     8581 aattaaccat gcccgcgtta atcagcagca catggatttc ctttttgttt tccgctttgg
     8641 caaattgctc aatcgttatg ggcgcattgg actttttgcc cttattcttt ttcgcgcttt
     8701 ccaccacata ggttttcagg cgcacgcctt cataatcgcc gccgaaatcc tgttcaaaat
     8761 gctctgccaa agccttgctt tgcaaaaact gccgcgttcc cgccttaatg gacaaagtcg
     8821 gcacgaccac gataaatttg aacacgccca gccaacggtg cagctcgaac atggtttgtg
     8881 tgtaggtata ggttttgccc gtgcccgttt ccatggaaat atcaaggata ttttggtcgt
     8941 ccgaacggtc ggggaatcgg ccgtctatgc cgttttggct ttgcactttc aggatattgt
     9001 ctgcgtattg ttttgcagca aacaaaagtt cgggattttc gtctgccgcc cgatatttgg
     9061 gcgttgcccc gtcaaacacg cccaaaaccg ccgaaaccgc ccgcatttgg tgcggctggt
     9121 ttttctcgta attaaaacca ctcataaact ttctccgtca aaccctgata atcacgttta
     9181 actcaatttc ttttttattg gcataaccgc gaaccgcctg gtcaagttcg tgctgcatgg
     9241 cgcttgccat attgctgccg aatacaatca cgcggttggg attgaaatcc gcatcgtcgt
     9301 ccagcttgcg gataaacgcc aacaaatcgg cggaagtaaa accggcattc atcagataca
     9361 gccgtttttc gcacagatac gccgtgtaag cccctaaccg cacaggctca accggcgtgg
     9421 tcagtgccgc cccgtcatac agcgtccagg tggtcagaag cgtttgcagc tgttcttcgc
     9481 ttaattcatc gttaagcggc aaatccggtt gttcgggcga aaaatccttg tccggatgct
     9541 gcctgaaatt gtctgccgtt tgaaagattt tgaagcccga atcgcccgtg taatcgggat
     9601 gttcgacgcg gattttggcg gcggcttttt ctatgcgggc tttggtgatg tcgaagatgg
     9661 tcgggtagcc tgctttacgg gcttcggatt tttcagcggt tttttcggga agctgtacac
     9721 agatatagcg gcggttaccg ttttgtcctt cggcgttaag ctgcatcacg gcgtgggcgg
     9781 ttgtgccgct gcctgcgaag aagtctagga ttaggtcagt ttgatttccc ttgaaagttg
     9841 cagaaagaac taatcttttc aataaatcta atggttttgg tgtttcaaaa ggagaataac
     9901 cttttccagt ttcaaacaac tcttctaatt cacgaatacc atctttagta agatttttac
     9961 ttatatttcc aatatttaag atgctatgaa atttggaaaa atcatcggat acttttctaa
    10021 gttttccaat aattgttttc ccataagact tcggaattaa gatatattca ttattttcaa
    10081 tatctttatc aattttgttt ttaaactcaa ttccttttat ttcaataata tcttcgtaat
    10141 gcaagttccc atatttgcca aactgttcgc gaacggcatc ttcctgtatc caatataatt
    10201 catcattgat tacaattttt ttgccgcgta tatcttttgg tctttttaga ttgtcaaaat
    10261 gagtaatatc tttcgcataa actaaaacag tttcatgccc atttacaaca tattttgcat
    10321 taccaccacc ttctgatgta ttaacaatta aatctttaat aaaattccct tccccaaaca
    10381 cttcatcaca caacaacttc aactgcgccg cttcgttatc gtcaatcgag ataaaaatca
    10441 cgccgtcgtc ctttaacagt tcgcgggcga tatacaggcg cggatacata aaggtcaacc
    10501 atgcactgtg cgagtttgag cctttgtcgg tgaaatctaa aatccgcgcg gcttcgtctt
    10561 cgtcaatatt ggctaagcag gcaagttcgg ctggtgtgaa tttgcggtcg tcctgataga
    10621 caaagccgtc tgatccggtg ttgtagggcg ggtcgatgta aatcatcttc acgctgtttg
    10681 tgtaggcgtt ttttaagtgt ttcaacactt ctagattgtc gccacgaatc agtaggtttt
    10741 ggctgcctgc attttcgggt ttggcgttgt gcgtcttgtc ttcacttatc agggtttcgg
    10801 gcggcaggtt gcgaagcagg cgagcatatg atttgcccag ccagttcatt tcgtaaaatt
    10861 cgcgtccgat gtcggtctgt ggcgcaattt cggcttgtaa tctgtcgata aggaaatttc
    10921 cgtctgcgtc aaaacaggcg ggaaacagtt ttttgagctg ttcgagttgg gtagagttgg
    10981 cggtaatgcc gtctgaagtg tagattgcct cggtgttcac cccggctgtg tcgattagat
    11041 caggg
//
LOCUS       DAXHMX010000064        10895 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHMX010000064 DAXHMX010000000
VERSION     DAXHMX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.064"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(140..1285)
                     /gene="dapE"
                     /locus_tag="ACFM4S_001195"
     CDS             complement(140..1285)
                     /gene="dapE"
                     /locus_tag="ACFM4S_001195"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6050877.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(1398..2018)
                     /locus_tag="ACFM4S_001196"
     CDS             complement(1398..2018)
                     /locus_tag="ACFM4S_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFC6050878.1"
                     /translation="MLTSKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQVWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     KLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(2043..2552)
                     /locus_tag="ACFM4S_001197"
     CDS             complement(2043..2552)
                     /locus_tag="ACFM4S_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6050879.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQITRYFNVAAPLHHEDEEEDFFPLLLQYAPQAREGVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDAEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(2647..3198)
                     /gene="azu"
                     /locus_tag="ACFM4S_001198"
     CDS             complement(2647..3198)
                     /gene="azu"
                     /locus_tag="ACFM4S_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6050880.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(3479..3901)
                     /locus_tag="ACFM4S_001199"
     CDS             complement(3479..3901)
                     /locus_tag="ACFM4S_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6050881.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDVSIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            4033..6783
                     /gene="secA"
                     /locus_tag="ACFM4S_001200"
     CDS             4033..6783
                     /gene="secA"
                     /locus_tag="ACFM4S_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6050882.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            6927..8699
                     /gene="dnaG"
                     /locus_tag="ACFM4S_001201"
     CDS             6927..8699
                     /gene="dnaG"
                     /locus_tag="ACFM4S_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6050883.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAEAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            8885..10813
                     /gene="rpoD"
                     /locus_tag="ACFM4S_001202"
     CDS             8885..10813
                     /gene="rpoD"
                     /locus_tag="ACFM4S_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6050884.1"
                     /translation="MSRNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         2849 a         3037 c         2691 g         2318 t
ORIGIN      
        1 ccctaaccct ctcccacggg agagggaatg gattgccgtt gaaataaatc gctctacata
       61 aaaaatcaat gtgttatctc aaacccacgt taggtctaag caaatggtcg gacatccgta
      121 tccggcaagc aagctgcttt cagacggcat ttccagccaa caaccgcgcc aatatcccct
      181 catacaccgc agacagcttc ggaatgtcgt ttagccgcac gttttcgttg atttggtgga
      241 tggtcgcatt ggacgggcct aattcgatga gttcttttgc aatggctttg atgaagcgtc
      301 cgtccgaagt gccgccggtg gtggacaatt cggtctctac gccgcagatt tcggcaatgg
      361 cggcgcgtgc cacgtcggtc agtttgcccg cgtgggtcag gaagggctgc cccgaacacg
      421 accactgcaa atcgtattgc acaccgtgtt tgtccaaaat ggcgtggacg cgttgtttca
      481 gccctgcttc ggtggactcg gtggagaagc ggaaattgaa tttgacgctc agctcgcccg
      541 gaatgacatt ggtcgcgcct gtaccgccgt tgatattgga aatttgaaag ctggtcggcg
      601 ggaagtattt gttgccttcg tcccagactt cctgcgtcag ctctaacaag gccggggcaa
      661 aagtatgcac gggattgatt gccaaatgcg gataggcaat atggccttgc ttgcctttga
      721 cggtcaggtt gcccgacagc gagccgcgcc ggccgttttt aatcatatcg cccaatttgt
      781 ccacggctgt cggttcgccg acgatgcagt agtcgataag ctcgtcgcgc gctttcaata
      841 catcgacgac tttggtcgtg ccgtccaacg cgtcgccctc ttcgtcggaa gtaatcagaa
      901 gcgcaatgct gccttggtgg tcgggatgtt cggcaacgaa gcgttcacag gcggtaacga
      961 agcaggcgat gctggttttc atgtctgccg cgccgcgccc gtataatctt ccgtcgcgct
     1021 cggccggttc gaacgggggc gaatcccatt tttcgacagg acctgtcggt acaacgtcgg
     1081 tatgccctgc aaaacagacg acgggagctt tcgtgccgcg tcgcaaccag atgtttttgg
     1141 tgtcgccgaa atggagttct tcagccgcaa aaccgatttt gtgcaggcgt tcggcaagga
     1201 gtttttggca atccctgtcg tcaggggtaa cggatggtcg ggaaatcagc tctttggcaa
     1261 gctctaggga ttgagtttcg gtcatatttg ttcacttttg aaattagacc gtctgaaacg
     1321 ttctgaatgt gattttcaga cggcatttag gttaggttgg catacggggt gggtatttta
     1381 cccatcagtc ttctgaatca tttgccgtgg caggcttcgt aaagcggcag caaatcttcc
     1441 accgtttccg ctatccattt cgcgacatcc tgcttgccca aatcgtcgcg ctcgatgtgt
     1501 ttgccgatgc agaaaaagtc ttcgtcgttt tgcaactttc ggtcggattc gctttgttgt
     1561 gcgacggtgc ggtagtcgtc atattcgctt tccgcgccgt gccacatatc gaatgcggcg
     1621 tatttgtcgg tatcgaaatc atccaaccag cggttgtatt cgggcagcgc aatcggcgac
     1681 acgtcggctt tgtagcagtg ccaatccaag ctgacgctca ggcggcggcg gttcaataaa
     1741 atcgataaaa tcgcggcgga atttttatat tgttcgtatt tgaagtaggc aaagaaatgg
     1801 gcgcgcacct gccagccgtt acaccagcgt tcgatatgcg gcggcgcgaa cggcgcaccc
     1861 aattcggcgg caacctgctg gatcagctgc tgccatacct gccagttttc tttatagtct
     1921 gccttgattt gcggaatgct ttcaggctgg tattttttaa gctgggaaaa ttggaaaaac
     1981 gggatattga acaaatcgca acttttcgag gtaagcatag tgtattcctt gagacgatta
     2041 ccttatttgc gccgacgcgc cgccatgatt tcaccgattt cggtcagttt ttctttaggg
     2101 ataaaggtgt tgcccatatc aaacagcggc tcttcaatcg ccaaatgaac atcatatccc
     2161 gccacaaaac gtttgaacgc ttccgcatcg ggaacataag cattgtctgc ttcgagcttg
     2221 gcaaattcgg cggaaacagc cgcccagttg tcgtgcagcc cgatatgttg gcgtaaaagc
     2281 tcgtccacac cttcacgtgc ttgcggcgcg tattgcagca gcagcgggaa aaagtcttct
     2341 tcttcgtcct catgatgcag cggcgcggca acgttgaaat accgggtgat ttggcggatg
     2401 gtttgcaaaa caatttgatt gcagccgttt tcggcgatgt agtccgacag catggcgact
     2461 tgtccgcaaa aacggcgcac tttgccgtgg caggcataca gcatttcaat cggttcggca
     2521 aaggtaacgc ttttggtttc aaacggattc atgttttcgt tctcaacggg cacttttcaa
     2581 gcagtcattt tataataaaa cagcctgcac aaagcaggct gtccgtcttt tgagacttta
     2641 agcggattaa tcgaccaaag tcactttgcc gttcatcaaa gcaccgtgac ctgggaaggt
     2701 acaagcgaat ttatattcgc cgtcggccaa tttagcagga tccagagtca gggaagcttc
     2761 ttcgccgccg ccgatcagtt tggtatgggc aacaacgcgt gcatcatcag gtttgacata
     2821 gtcggtatcg gcagcaccta cgccgtcttt aaatacgccg tccatgtctt cagctttggc
     2881 aatcacgaga ttgtgaccca tgctggcttt gggttgcgta ccggtatgtt tcagagtgat
     2941 ggtaaactct ttacatgctt tgctgacttg gatgtctttg gtgttgaact gcatattgtc
     3001 gttggattcg acagttgccg cacagttgcc ggcagcaggg gcttcggcag catctgcagg
     3061 agcagcttcg gcggcaggcg cttcggaagc gggtgcttca gcagcaggag ttgcctcggc
     3121 agcaggcgcg gcaggttctt gagagcaggc agccaaaccg ataacggcgg cagaaatcag
     3181 agccagatac gctttcataa caaatctcca atcgataaaa taatattcgg ttttacggaa
     3241 atcaaagtgc aaccgccatt aacaaaacct tgaaaaagat tccgccgcgt tgcacaaaca
     3301 gatgtttcgg agcggcattt tgctacaaat ttcatttgaa atcaaagcct gtctgcaagt
     3361 ttacaatcgt ttactttaaa aggggcaatt ttaccccgaa cctatttctt tagtattaga
     3421 cccattatcc tttacttctt aatattaacg gatgtttaca caaattcccg tatacatttt
     3481 atgcgccatg ccttctaacc aagtttgcca atgcctccgc caattcggga tgccgttttt
     3541 ccaactttgc cgccgccgaa ccgaaactct ccagcgcagc cttactcaaa tgcagggtat
     3601 tggttttcgg cggtttttcc ggtttcggga ccagcctgac cgaaacagag cgtatcgaaa
     3661 catcaagccc tgccaactgc ggcaataccg acggtgcaat cattttcaaa cgcgatgccg
     3721 ccatattgtt tgccgccaaa aggacaagcc tgccgtcttc gatacatgcc gtctgaaaat
     3781 gcgggtgcag gttggcaggc agcagttttt tcacggcggc atccaaccgc cgccactgtc
     3841 ccgcctgttt caaaagtccg gaaagcagcg cgtcccgcct gcccaactgt tccaaattca
     3901 taaaacatac acccaaaaag attgaaatac cgcaaacgcg cctttatttc agacggcatt
     3961 agcactttgc acaaacgctt gtgttaaaat cgcgttttcg ccccactatt atatcaggcg
     4021 caggaattat tcatgctgac aaacattgcc aagaaaatct tcggcagccg caacgaccgc
     4081 ttgctgaaac aataccgtaa atccgttgcc agaatcaacg cgctcgaaga acagatgcaa
     4141 gccctaagcg atgctgatct gcaagccaaa actgccgaat tcaaacaacg cctcgccgac
     4201 ggtcagactt tggacggcat tttgcccgaa gccttcgccg tctgccgcga agcgtcccgc
     4261 cgcaccctcg gtatgcgcca cttcgacgtg cagcttatcg gcggtatggt gctgcacgac
     4321 ggcaaaatcg ccgaaatgcg taccggcgaa ggcaaaaccc tggtcgccac cctcgccgtc
     4381 tatctcaacg cgctggccgg caaaggcgta cacgtcgtta ccgtcaacga ctacctcgcc
     4441 tcacgcgatg cgggcattat ggagccgctc tacaatttcc tcggccttac cgtgggcgtg
     4501 attatttcag atatgcagcc gttcgaccgt caaaacgcct atgccgccga tatcacctac
     4561 ggcaccaata atgaattcgg cttcgactac ctgcgcgaca atatggttac cgaccaatac
     4621 gacaaagtgc agcgcgaatt gaattttgcc gttgtcgatg aagtggattc catcttgatt
     4681 gacgaagcgc gcactccgct gattatctcc ggtcaggcgg atgacaacat ccagttgtac
     4741 caaatcatga acaccgttcc gccccacctc gtccgtcaag agacagaaga aggcgaaggc
     4801 gactattggg tcgacgaaaa ggcacatcag gtcatcctga gcgaagcagg tcacgaacac
     4861 gccgagcaaa tcctgaccca aatgggattg ctggcagaaa acgactccct ctattccgcc
     4921 gccaatatcg ccctgatgca ccaccttatg gcggcattgc gcgcgcattc cctcttccac
     4981 aaagaccaac attacgtcat ccaagacggc gaaatcgtca tcgtggacga attcaccggc
     5041 cggctgatgt ccggccgccg ctggtcggag ggtctgcatc aagccgtcga agccaaagaa
     5101 ggcgtggaaa tcaaacgcga aaaccaaacc cttgcctcca tcaccttcca aaactatttc
     5161 cgcctgtaca ccaagctctc cggcatgacc ggcacagccg ataccgaagc tttcgagttc
     5221 caaagcatct acaacctcga aaccgtcatc atcccgacca accgccctgt acagcgcaaa
     5281 gacttcaacg accagatttt ccgttccgcc gaagaaaaat tcgaagccgt cgtcaaagac
     5341 atcgaagaat gccacaaacg cgggcagccc gtcctcgtcg gcaccaccag catcgaaaac
     5401 tccgaactgg tctcccacct tctgcaaaaa gccggactgc cgcacaacgt cctcaacgcc
     5461 aaagaacacg aacgcgaagc cctgattgtc gcccaagccg gcaaagtcgg cgcgattacc
     5521 gttgccacca atatggcggg acgcggtacg gacatcgttt taggcggcaa cctgaagcac
     5581 caaaccgatg ccatccgtgc cgacgaaacc ttgagcgacg aagagaaaca ggcacaaatc
     5641 gccgcactcg aaaacggctg gcaggcagaa cacgataaag taatggaagc aggcggcctg
     5701 cacatcatcg gtacggaacg ccacgaaagc cgccgtatcg acaaccaatt gcgcggacgt
     5761 tccggccgtc agggcgaccc cggatccagc cgtttctacc tttcgtttga agacccgctg
     5821 ctgcgcttat tcgcactcga ccgcgccgcc gccatcctca accgtctcgc ccccgaacgc
     5881 ggcgtcgcca tcgaacacaa cctgctgacg cgccaaatcg aaggggcgca acgcaaagtc
     5941 gaaggcagaa acttcgatat gcgcaaacag gttttggaat acgacgacgt tgccaacgaa
     6001 cagcgcaaag tcatttacag ccagcgcaac gaaattctga ccagcaaaga catcagcgac
     6061 ctgatgcagg aaatccgttc tgatgtcgtc agcgacctcg tggataccta tatgccgccc
     6121 gacagcatgg aagaacaatg ggacatcccg actttggaga accgtctggc tgccgaattc
     6181 agactgcacg aagacatcca atcctggctg aaggcggaca atgcgattga cggtcaagac
     6241 atcaaagaac gcctgatcga acgcatcgaa aacgaatatg ccgccaaaac cgaactggtc
     6301 ggcaagcagg caatggccga tttcgagcgc aacgtgatgt tgcaggtcat cgacaaccaa
     6361 tggcgcgaac acctcgccgc tatggactac ctgcgacaag gcatacacct gcgcagctat
     6421 gcccaaaaaa atccgaagca ggaatataaa cgtgaagcct ttaccatgtt ccaagacctg
     6481 tggaacggca tcaaattcca tattgcctcc ctgcttacct cggttcaaat cgaacaaaac
     6541 cctgtcgcgg tggttgaaga gcaacccatc ggcaacatcc agtccatcca ttccgaatcg
     6601 cccgatatgg aagaactttt gggtcagtcg caaaccgatt tggttaccga ggcctttaat
     6661 cccgatggaa cggatttcag ccccgaagcc ttggaagcgc ggggacaaat cgtccaccgc
     6721 aacgacccct gcccctgcgg cagcggtttg aaatacaaac aatgccacgg caaactggct
     6781 tgagcgtttg aacgcaaatg ccgtctgaac atccggtttc cggcttcaga cggcattttg
     6841 cctgaaccgc cacatccgac cgccattccg aaaaatcccg atttcgtacc gtccaaacta
     6901 aaaacaggca tccgccccgc cgaactatga ttccatccga cttcattgac gaacttctgg
     6961 ccaaaaccga tattgtcgac atcatcgacg aacaggttcc gctgaaaaaa ggcggggcga
     7021 actatatggc gtgttgcccg ttccacaagg aaaaaacgcc gtcgttttcg gtcagtccga
     7081 ccaagcagtt ttaccactgt ttcagttgcg gggcgcacgg ttcggcgatt ggttttgtga
     7141 tggaacatca ggggctgtcg tttccggagg cagtgcagtt ccttgccgac cgcgtgggta
     7201 tgattgtgcc taaagtgcgc gggcagaacg ataatcccga agtccgtgcc gaacgtaaga
     7261 aaaagcagca gacgctggaa gaaacgacag cggaggcggc tgatttttat gcgcaacagt
     7321 tgaaattcaa tccgacggcg aaggcttatt tggacaagcg cggcttgagc gcggaagtca
     7381 tcgcgcatta tggtttgggc tacgcgcccg acggctggca gcctttggcg caagtattcc
     7441 aaccgtaccc gaataccgcg ttggtggata cgggtatggt gattgacaat gaggggcggt
     7501 attacgaccg attccgtcat cggattatgt tccccatccg caatccgcgc gggcaggtca
     7561 tcggtttcgg cggcagggtg ctggacgact ccaaacccaa atatctaaac tctcccgata
     7621 cgcctttgtt tgataagggg aaaaaccttt acggcttgta tgaagggcgt gctgctgtca
     7681 aggaagtggg gcggattctg gtggtcgaag gctatatgga cgtggtcgcg ctggcacagt
     7741 tcggcgtggg ctacggcgtg gcggccttgg gcacggcaac aacagcggaa cacgtcaaaa
     7801 tcctgatgcg tcaggcggac agtatttatt tctgtttcga cggcgacagc gcggggcgca
     7861 aagcggcttg gcgcgcgctg gaaaacgcgc tgccgcagtt gaaggacgac aaatcgctgc
     7921 attttttgtt cctgccggaa gaacacgacc ccgacagtta catccgtgcc tacggcaaag
     7981 cgcaatttga agacgcgctt ctgaatcaaa gcaagccttt gtcggagtat ttctgggaac
     8041 acctttcaga cggcattcat ctcaatacgc aggaaggcaa ggcggaattg gtgaaaacca
     8101 gttcgccgct tttggcgcag attaccgcgc cggcattggc ttatttgtta aaacaacggc
     8161 ttagcgaact ggtcggcatc gaccccgaca acctcgcgca actgctggga caggaagcgc
     8221 cgaagcggca cgtcaaacaa aaaaactaca aactgccccc gatttccgtc aaacagcccg
     8281 ttatgccgac attggtgcaa cggcaaatcc gcagcctctt gataaatccg gattgggctg
     8341 catatataga cctgcccgat tatctggcgt tggacggcga tttcgcctgc cttgccaacc
     8401 tcgccgaaac cattaaaaac catccttccg tgccggcaac cgcacaggtt ttggaacata
     8461 tgcgcggctc gccttacgaa gaaacgataa cccgaatctt ccattcaacg caccaatcgg
     8521 aagaaatgaa cagcagcagt gaagaagatt gcgagaattt ccaaatcggc atgaaaaaac
     8581 tgctcaatga gttaaaatac agccaaatcg aaacattaaa acaaaaaagc ctgcaatccg
     8641 gcttaaatga aagcgagaaa aaacttttgc tgtcgctgct gaccgcaaaa caaaattgac
     8701 cggcggattc cgccatccgt aaaccgttat gccgtctgaa aagcacttac cccggctgca
     8761 acaacgacac ctgcagaaca cccatcccaa aaagccttca gacggcatca gagtacccgc
     8821 tctgccacgc cttcaggtgc gttcaaacgc aaaccgtcgg caccttacca acagaaagca
     8881 gacaatgtcc agaaaccaaa atcacgaaga ataccaagac gatgcccgcc ccctgacgat
     8941 tgaggaacaa cgcgctcgcc tgcgccaact catcattatg ggcaaagaac gcggctacat
     9001 cacctactcc gaaatcaacg acgcactgcc cgacgatatg tccgatgccg atcaaatcga
     9061 caacatcgtc agtatgattt ccggtttggg tatccaagtt accgaacacg cccccgatgc
     9121 ggaagacata ttgttaagcg acaatgccgc cgttaccgac gatgatgccg tcgaagaagc
     9181 cgaggccgcc ctttccagcg cagattccga gttcggcagg acgaccgacc ccgtccgtat
     9241 gtatatgcgc gaaatgggac aggtcgacct gctgacccgc gaagacgaaa tcatcatcgc
     9301 caaaaaaatc gaaaacgccc tgaaaaatat ggttcaggcc atctccgcct gcccgggttc
     9361 cattgcggaa atcttagaac tcatcgaaaa aatccgcaaa gacgaaatcc gcgtcgacga
     9421 agtcgtagaa gccattatcg acccgaatga agtattgctc aacgaattgg gcttggggca
     9481 cttggaaacc acagcacccg agaaaccttc caacgacaat tcggacgaaa acgaagacga
     9541 cgaagaatcg gaagaagatg cggatgaaat ctcggcagcc aatctcgccg aattgaaaca
     9601 aaaagtcatc ggccactttg cccaaatcga aaaagactac aaaaaaatga ttggccgttt
     9661 ggaaaaacac cacagccggc acaaagacta tctcgcctac cgcgacgcga ttgccaacaa
     9721 acttttggaa gtccgcttcg ccacccggca aatcgacagc ctcagcagca gcctgcgcgg
     9781 aaaagtagaa aacatccgca aactcgaacg cgaaatccgc gacatctgcc tcgaccgcgt
     9841 ccatatggag cgcgactact tcatccaaaa cttcctgccc gaaatcacca atctggaatg
     9901 gattgaagaa gaaatcgcca aaggcagggt ttggagcgac gcgctcgacc gcttccgcca
     9961 cgccatcctc gaaaaacaaa ccgagttggc ggatatggaa aaagaaaccc gcatttccat
    10021 cgaagagttg aaagaaatca acaaaaatat ggtgtcaagc gaaaaagaaa ccgcagctgc
    10081 caaacaggaa atgattcagg caaacttgcg cctcgtcatt tccattgcca aaaaatacac
    10141 caaccgtggc ttgcagttcc ttgatttgat tcaggaaggc aacatcggcc tgatgaaagc
    10201 ggtcgacaag ttcgaatacc gacgcggcta taagttctcc acctacgcaa cctggtggat
    10261 ccgtcaggca atcacccgct cgattgccga tcaggcgcgt accatccgca ttccggtaca
    10321 tatgattgaa accatcaaca agatgaaccg catctcgcgc caacaccttc aagaaaccgg
    10381 cgaagaaccc gattccgcca aacttgccga actgatgcag atgcccgaag acaaaatccg
    10441 caaaatcatg aaaatcgcca aagagccgat ttcgatggaa acccccatcg gcgacgacga
    10501 cgattcgcac ttgggcgact tcatcgaaga tgccaacaat gttgcgccgg ccgatgcggc
    10561 aatgtacacc agcctgcacg aagtaaccaa agaaatcctc gaaagcctga caccgcgtga
    10621 ggcaaaagtc ctgcgtatgc gtttcggcat cgatatgaac accgaccaca cgctggaaga
    10681 agtcggcaga cagtttgacg taacgcgcga acgcatccga caaatcgagg caaaagcact
    10741 ccgcaaatta cgccatccga caagaagcga ccgtttgaga agtttcttgg acagcgaaga
    10801 cagcaagctg taaaccaaaa aaccgcaggt ttcaaatacc tgcggttttt tcttacacaa
    10861 taaacaacgc ttccacatat cccacactcc tatcc
//
LOCUS       DAXHMX010000065        10817 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHMX010000065 DAXHMX010000000
VERSION     DAXHMX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.065"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..716
                     /locus_tag="ACFM4S_001203"
     CDS             <1..716
                     /locus_tag="ACFM4S_001203"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFC6050885.1"
                     /translation="QQMPSEQHEAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLEELVPN
                     KPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLNN
                     QTWWSRVLDGSYNRERLGS"
     gene            929..1804
                     /gene="rfbA"
                     /locus_tag="ACFM4S_001204"
     CDS             929..1804
                     /gene="rfbA"
                     /locus_tag="ACFM4S_001204"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6050886.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            1859..2398
                     /gene="rfbC"
                     /locus_tag="ACFM4S_001205"
     CDS             1859..2398
                     /gene="rfbC"
                     /locus_tag="ACFM4S_001205"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6050887.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            2560..4608
                     /locus_tag="ACFM4S_001206"
     CDS             2560..4608
                     /locus_tag="ACFM4S_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC6050888.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPKIDSL
                     VQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLAGVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAETPSRLEYILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYR
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQTDILTCLQY
                     ADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGYFAKKLGKIKQLYRSFK
                     "
     gene            4745..6004
                     /locus_tag="ACFM4S_001207"
     CDS             4745..6004
                     /locus_tag="ACFM4S_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC6050889.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            6371..7777
                     /locus_tag="ACFM4S_001208"
     CDS             6371..7777
                     /locus_tag="ACFM4S_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6050890.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAEFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAISNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            7995..9209
                     /gene="gltS"
                     /locus_tag="ACFM4S_001209"
     CDS             7995..9209
                     /gene="gltS"
                     /locus_tag="ACFM4S_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6050891.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            9308..10324
                     /locus_tag="ACFM4S_001210"
     CDS             9308..10324
                     /locus_tag="ACFM4S_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6050892.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            10335..10532
                     /locus_tag="ACFM4S_001211"
     CDS             10335..10532
                     /locus_tag="ACFM4S_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050893.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            10550..10699
                     /locus_tag="ACFM4S_001212"
     CDS             10550..10699
                     /locus_tag="ACFM4S_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050894.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHRTIQKAS
                     PYVPS"
BASE COUNT         2794 a         2953 c         2532 g         2538 t
ORIGIN      
        1 ggcaacaaat gccgtctgaa cagcacgaag ccttccgttt tcatcatatt tcgactgatg
       61 aagtgtacgg cgatttgcac ggcacggatg atttgtttac cgaaaccacg ccttacgccc
      121 cttccagtcc ttattcggct tccaaagcct ccagcgacca tttggtgcgc gcatggctgc
      181 gtacttacgg cctgccgacc attgtgacca attgttctaa taactacggc ccttatcatt
      241 ttcctgaaaa actgattcca ctgatgattc tgaatgcgct tgatggcaaa ccactgccgg
      301 tgtatggcga cggtatgcaa atccgcgact ggctgtttgt tgaagatcat gcgcgtgcgc
      361 tgtatcaagt ggtcacagaa ggcgaaatcg gcgaaactta caacattggt ggccacaatg
      421 aaaaagccaa tattgaagtg gtgcgcacca tttgcgccct gctggaagaa ttggtgccga
      481 acaaaccgca aggcgtggcg cgttatgaag atttgattac ctatgtcaaa gaccgccccg
      541 gccatgatgt gcgctatgcg attgatgccg caaaaatcgg ccgtgagttg ggttggcagc
      601 cgcaagaaac ttttgaaagc ggcatccgca aaacagtgga atggtattta aacaatcaaa
      661 cttggtggtc gcgcgtgtta gacggcagct acaatcgtga acgcttaggc agttaaatag
      721 agacctttgc aaactcccca tctttggcac atttcttcgt tatgcgctgc tcgaaagctt
      781 gcctatcttt gtgatatgtc tgcgctttct gcgctgctat aacttcgaac tgtactcaaa
      841 tctgtggctt tgcaaaggcc tcaaataata aaaatacgtt cagacggcat gaaggccgcc
      901 tgaaaaacta cataaggaaa gtaaactgat gaaaggcatt attctcgcag gcggcagcgg
      961 tatccgcctt tacccgatta cccgcggcgt atcaaagcaa ttactaccgg tgtacaacaa
     1021 accgatgatt tattacccct tgtcggtgtt gatgttggcc ggaatccgcg atgtgctggt
     1081 gattaccacc cccgaagaca acgatgcttt ccgccgctta ctgggtgatg gcagcgattt
     1141 cggtattcaa atttcctacg ccatccaacc cagccccgat ggtttggcgc aagccttttt
     1201 aattggtgaa gaatttatcg gcgatgattc ggtgtgtttg gtgttgggcg acaatatttt
     1261 ctatggccag catttcagcc aatccctgaa agaagccgcc gcttccgtgg aaaccaaagg
     1321 tgctaccgta ttcggctatc aagtgaaaga tcccgagcgt ttcggtgtga ttgagtttga
     1381 tgagaatttc aaagccctgt caatcgaaga aaaaccgaaa caacctaaat ccgattgggc
     1441 ggtaaccggc ctctatttct acgataatcg cgtagtcgag tttgccaaac aagtgaagcc
     1501 gtctgaacgc ggcgaattgg aaattaccac cttgaatcaa atgtatttgg acgatggttc
     1561 gctcaatgtc caattgctcg gtcgcggttt cgcatggctt gataccggca cacacgccag
     1621 cctgttggaa gcagcctcgt ttgtcgaaac cattgaaaac attcaaaacc tacaagtcgc
     1681 ctgtttggaa gaaatcgcat ggcgtaatgg ctggttgagc agcgaagaaa tcagaaactt
     1741 ggcaaagcca atgtgtaaaa atgaatatgg ccgctattta ttgcgcttgg ttggcaaaga
     1801 ttaagaggtt gcttttacca gttttcaggc cgtctgaaac gctttttagg aaaacatgat
     1861 gaatatcatc gatacccgca ttcccgatgt caaattgctc gaacccaaag tcttcggtga
     1921 cgagcgtggt ttttttatgg aaaccttccg tgatgaatgg ttccgccaaa atgtagccga
     1981 gcgcactttc gtgcaggaaa accacagcaa atccacccaa ggcgtattac gcggtttaca
     2041 ttatcaaacc gaaaatacac aaggcaaact ggtgcgcgtg gtatcaggcg aagtctttga
     2101 tgttgccgtg gatatgcgtc gtgatagtcc tactttcggc cactgggtcg gtgaggtttt
     2161 atcggcagac aacaaacgcc aattatgggt gccggaaggg tttgcccatg gattttatgt
     2221 gttgagcgat gaagcggaat tcgtctacaa atgcaccgac tattacaacc cgaatgcgga
     2281 acattcccta atttggaatg atgccacagt gggaatcgac tggcctttat tgggagaacc
     2341 aagcctatcg gccaaggatt tggcaggcaa gatgcttgct gaagcggcca cattttaatt
     2401 ttcactttta ggtcatcgtg ggtaatttgt agcaagatga cctaattttt accagctagc
     2461 ctactaacta aatttctact atatttaaat agtcagaaaa tttacacaaa atctgttaat
     2521 aagatattat attgtcagta atattttttc ctatttattg tgattcctgc ctacattcct
     2581 cttcgctctt tcggtatacg taaaattccg aatattacgc aatttctacc ggaatattca
     2641 cttaacaaaa ccgtattcaa cgcccaaacc atcattggct ggggcttacg cccgaccacc
     2701 caccaagcac gtgcttttgc acaatcacat ggcttgccgt ttgtagcctt ggaagacggt
     2761 tttttacgct cactgggctt aggcgtggcc ggttatccgc ccttcgcact ggtttacgac
     2821 gatatcggca tctattacga cacaacccgc ccatcgcgtt tggagcagtt gattttagcc
     2881 gcaaacatgc cgtctgaaat gcttaaccaa gcgcagcagg ccattgattt gattttgacg
     2941 caccgtttgt ctaaatataa ccatgctctg gatttttcag acggcctcgt acaagacagc
     3001 aaaaacattg ttttggtaat tgatcaaact ttcggcgata tggcgattca atatggcggc
     3061 gcagatgaag ccacattccg ccaaatgttt cagacggcct tagccgaaaa tcctgatgcg
     3121 gaaatctggg tcaaaaccca tcccgatgta ttgagcggca agaaacaggg ttaccttact
     3181 gatttggcac agcagccgcg tgtgcgttta ttggctgaag acattaatcc gatttcgctg
     3241 ctgcaagcgg tggacaaagt gtattgcgtg acctcgcaaa tgggttttga agccttgttg
     3301 tgcggcaagc ctttggtaac gtttggtttg ccgtggtatg ccggatgggg cgtgagtgac
     3361 gaccgccatc ctaaaatcga cagccttgtt caaacccaac gccgcgcccc ccgcaacttg
     3421 ctgcagctct tcgctgcagc ctatctgcaa tacagccgct acctcaaccc caataccggc
     3481 gaagcaggca gcctctttga tgtcatcgac tatctggcga cggtcaaacg caaaaacgac
     3541 aaattgcgtg gcgagttgta ttgcgttggt atgtctttgt ggaaacgcgc ggttgccaaa
     3601 ccgttcttta acgtaccctc ttgccgtctg aaatttatct cttccaccca aaaactggca
     3661 ggggtcaaac tgtccgacga tgcacgcatc cttgcctggg gcaacggcaa agaggccatc
     3721 gtccgctttg ccgaacaaca ccacatcccc ctgctgcgca tggaagacgg ctttatccgc
     3781 tcggtcggac tcggctccaa cttagtgccg ccgctgtcgc tcgttaccga cgatatgagc
     3841 atttatttca atgccgaaac cccgtcccgt cttgaataca tcctacaaaa ccaaaacttc
     3901 gacgatcaag actttcagac ggccttgaag ctgcaaaaaa tgctgaccga aaaccacatc
     3961 agtaaataca acgtcggcag ctcagacttc accgccccgt caaccgacaa aaccgtgatc
     4021 ctcgttcccg gccaggttga agatgatgcg tctatccgct acggttcgcc ccaaatctac
     4081 cgcaatctgg atttgctccg taccgtacgc gaacgaaacc ccaatgccta tatcatctac
     4141 aaaccgcatc ccgatgtagt cagcggtaac cgcatcggcc atatttcccc tgaagatgct
     4201 gcacgatatg ccgaccaaac cgccgagcaa accgacatcc tgacctgtct ccaatacgca
     4261 gacgaagtac ataccatgac ttcgctgacc ggttttgaag ccttgttgcg cggcaaaaaa
     4321 gtcagctgct acggcctgcc tttttacgca ggctggggac ttacccaaga tctgctcccc
     4381 attccgcgcc gtagccgcag acttgagctt tggcagctga ttgccggcac gctcatccac
     4441 tatcccgact acatccaccc cgaaacccat caggccataa atgcagaaac cgcagcccaa
     4501 atcctgatac gacaaaaaaa tatgcaaaaa aacaacaacg gattacatcg cgggtacttt
     4561 gccaaaaaat taggtaaaat caaacaacta tatcgatctt tcaaataaat accatcaaag
     4621 ttaacgatgc gtcataaact tgcctctatt gcggcatcat tgcctttgca tcgttaattc
     4681 tcttggcgta tgcttgaaag ttcaacctaa aactattaca taaaaaacaa aaccacattg
     4741 caacatgaaa cagaccgtcc tcaaaaataa cctgcaaaac ctgcttgaaa gcgcagaaaa
     4801 tatcctgctg cttcaaggcc ctgtcggcga tttttttctg cgccttgccg actggctgac
     4861 tgcaaacggc aaaaccgtac ataaattcaa ctttaatgca ggcgacgact atttttatcc
     4921 gcccactcaa gcgcataccg ttgtttttaa cgacaactac gatgcctttc ctgagttttt
     4981 gcaagaatac atcactcaac atcacatcca ggccgttgtc tgctttggcg acacacgccc
     5041 ttatcacgtc attgcaaaac gcattgcaaa cgaaaaccaa gccagtttct gggcgtttga
     5101 agaaggctat ttccgcccct actacatcac cttagaaaaa gacggcgtca acgcattttc
     5161 cccgttgccg cgccgtgccg acttttttct tgaacaattc cctaagcttg cccagcaaga
     5221 atataaagcg ccaacgccgg tacacggcgg ttttacgccc atggcaaaaa acgctatccg
     5281 ttactatatc gagttgttcc gcaatccacg caaatacccc gactacatcc accaccgcgc
     5341 acccaatgcc ggccattacc tcaaaccgtg gtcgctctcc atcctcaagc gtttgaacta
     5401 ctatattgaa gacatccaaa tcgccaaacg tgtggaagca ggcaaatacg gcaagttttt
     5461 tattgttccc ttacaggtat tcaacgacag ccaagtccgt atccattgcg actttcccag
     5521 cgtccgcagc ttcctgctcc atgttttgag ttcatttgcc gagcacgcgc ctgccgatac
     5581 caacatcatc atcaagcatc atccgatgga ccgcggtttt atcgactact ggcgcgacat
     5641 taaacgcttt atcaaagaac accccgaact caaaggccgc gtgatttatg tccatgatgt
     5701 ccccctgccc gttttcctgc gccacggtct cggcatggtc accatcaaca gcaccagcgg
     5761 cctgtccgga ctgattcaca atatgccagt taaggttctc ggccgtgcct attatgatat
     5821 tcccggcatt actgaccaaa ataccttggc agaattttgg aatcatccga caccgcctga
     5881 caaagagctg ttccatgcct accgaatgta ccacctcaac gtgacccaaa ttaacggcaa
     5941 cttctacagt caggtgtttt tccccaacaa aaaaacctcc aactcttcca caccagtaat
     6001 ctgacttagc gaaggaagtt caggccgtct gaaaacattt cagacggcct gaaacaatca
     6061 ataccttagc tactgccatg taaataaaac acaaaaatct gcatttatca ttaacaataa
     6121 attacaaaaa cagtataatg accgagctgc catgagcgca taccgactca agctgaggcc
     6181 ttaataacac acaaaatatg gatgtactcc caggcaaaac aatataacaa gccaaacatc
     6241 ctaaagataa gccagcaagg caatacactt tacaaaactg tggcgagcaa tatttttaca
     6301 aagccctcaa ccggtatcgc cgcccatatg ccgcagcatc cgtcttccac tttatatccg
     6361 cccgcaaacc atgaccgccg ctcctgatat cctctaccgg caagccgccg cccttttgga
     6421 acaatccaat accgcccaag ccctgcccct gttgcaacag gcggcagagc aaggttatgc
     6481 ggaagctgct ttcgtattgg gcaaccatct gctgcaaaac ggccaaccgg agcaggcact
     6541 ttcatggttg gaagccgccg cggcccaacg ccatcccaaa gcactcttct ccctgctgca
     6601 acaacgcgaa cacaacggca ccccgaccgg acagcttctc aacgactatg cctggctggg
     6661 tgagcagggg cactcagaag cccaattaat cctcatgcgt taccacgcgc aacgcaacga
     6721 tccacaatcg ctctactggg cggaacttgc tgccgcccga tatgccgcac ctgcgtatta
     6781 ccatctggca cgccatcatc aacgccaagg cgacgttgaa acagccatcg aacaatacga
     6841 aaaagcggca gcactcggcg taactgccgc ctgctggcaa cttggtcaaa tctacttcta
     6901 cggtacaggt gtcagcccca accacgcaca agccgaacac tatctcgaac cagccgcaca
     6961 agccggccac atcgccgcac aaaccctgct ggccgacctt cttgccgccc aacgcaaacc
     7021 cgaagcctta gaatggtatc gtcgtgccgc caataaggag caagcggaag cacaagccaa
     7081 gctggcccaa tacgccctga ccggcgaact ttccgaacgc gatccgttcc aagcggcgcg
     7141 ctatgccaaa gccgctgccg agaaaaacca tcctgaagcc ctgaaaatca tgggcgacct
     7201 ctaccgctac ggcctcggca tcaaagctga caaccatatc gcgcacgatt actaccaccg
     7261 tgctgccgcg ctgggttctg ccgccgcagc acaaaaactc atcagcgacg ccgcgctgta
     7321 ccatccgcaa caatacgaac aaatcaaaac tgccgccctg caacagcaac aaaccgaaac
     7381 catctaccgt ttggcggaaa cacaagccca tgccatcggt cgtcccgccg actataatgc
     7441 cgcacgcaaa aattacatgg aagctgctga gttccaccat aaaaacgcgg cggctgcctt
     7501 aggccgcatc taccattacg gcctcggtac ggcgcaagat cctcgggcgg ctgcacactg
     7561 gtacgccatc gctgccgaac aaaaccaccc ttccgcccaa taccacctcg cctgttttta
     7621 ctatcacgga caaggtgtcg gctgtcatgt tccgaccgcc tgctactggc tgcaggctgc
     7681 catcagcaac ggccacactt cggccgaatc attaatatcc ctattagaac aatggcgacg
     7741 cgaagcacac catgccatcg gacaaaaggc cgtctgaaaa gatttacact cgcatttttt
     7801 gacaatcttt aactattccc ctaatatttg ccagttattt ttcacggaca cgccattgtt
     7861 ttcatttctt tctgaaaaca ccttgtccgc gcatcaatac catgacactc ggcggataac
     7921 gccaagcgtt gaaacacact acatccggaa caaaaacgga tgctcggaaa aatatttcta
     7981 ggaggtgaaa caacatggaa tgggaattca acagttatta cacactgatt gccgccacgc
     8041 tcgtgttgct ggttggtaaa tttctggttc aaaaaatcaa attcttacga gacttcaata
     8101 ttcccgagcc ggtcgctggc ggtttggttg ccgcgattat cctgtttgcg ctgcacgagg
     8161 cgtacggcgt gagcttcaaa tttgagaaac cgctgcaaaa tgcgtttatg ctgattttct
     8221 tcacgtccat cggcttgagc gcggattttt cccgtttgaa ggcgggcggt ttgccgctag
     8281 tggtttttac cgcgattgtg ggcggattta tcttggtgca aaactttgtc ggggtcggac
     8341 tggctacggc tttgggtttg gacccgctca tcggtctgat taccggttcg gtgtcgctga
     8401 cgggcggaca cggtacgtca ggtgcgtggg gacctaattt tgaaacgcaa tacggcttgg
     8461 tcggcgcaac cggtttgggt attgcatcgg ctactttcgg gctggtgttc ggcggcctga
     8521 tcggtgggcc ggttgcgcgc cgcctgatca acaaaatggg ccgcaaaccg gttgaaaaca
     8581 aaaaacagga tcaggacgac aacgcggacg acgtgttcga gcaggcaaaa cgcacccgcc
     8641 tgattacggc ggaatctgcc gttgaaacgc ttgccatgtt tgccgcgtgt ttggcgtttg
     8701 ccgagattat ggacggcttc gacaaagaat atctgttcga cctgcccaaa ttcgtgtggt
     8761 gtctgtttgg cggcgtggtc atccgcaaca tcctcactgc cgcattcaag gtcaatatgt
     8821 tcgaccgcgc catcgatgtg ttcggcaatg cttcgctttc gcttttcttg gcaatggcgt
     8881 tgctgaattt gaaactgtgg gagctgaccg gtttggcggg gcctgtaacc gtgattcttg
     8941 ccgtacaaac cgtggtgatg gttttgtacg cgacttttgt tacctatgtc tttatggggc
     9001 gcgactatga tgcggcagta ttggctgccg gccattgcgg tttcggcttg ggtgcaacgc
     9061 cgacggcggt ggcaaatatg cagtccgtca cgcatacttt cggcgcgtcg cataaggcgt
     9121 ttttgattgt gcctatggtc ggcgcgttct tcgtcgattt gattaatgcc gcgattctca
     9181 ccggttttgt gaatttcttt aaaggctgat tttccgcctt tccgacaaag cacctgcaag
     9241 gtttaccgcc tgcaggtgct tttgctatga tagccgctat cggtctgcac cgtttggaag
     9301 gaacatcatg tatcggaaac tcattgcgct gccgtttgcc ctgctgcttg ccgcttgcgg
     9361 cagggaagaa ccgcccaagg cattggaatg cgccaacccc gccgtgttgc aaggcatacg
     9421 cggcaatatt caggaaacgc tcacgcagga agcgcgttct ttcgcgcgcg aagacggcag
     9481 gcagtttgtc gatgccgaca aaattatcgc cgccgcctac ggtttggcgt tttctttgga
     9541 acacgcttcg gaaacgcagg aaggcgggcg cacgttctgt atcgccgatt tgaacattac
     9601 cgtgccgtct gaaacgcttg ccgatgccaa ggcaaacagc cccctgctgt acggggaaac
     9661 cgctttgtcg gatattgtgc ggcagaagac gggcggcaat gtcgagttta aagacggcgt
     9721 attgacggca gccgtccgct tcctgcccgt caaagacggt cagacggcat ttgtcgacaa
     9781 cacggtcggt atggcggcgc aaacgctgtc tgtcgcgttg ctgccttacg gcgtgaagag
     9841 catcgtgatg atagacggca aggcggtgaa aaaagaagac gcggtcagga ttttgagcgg
     9901 aaaagcccgt gaagaagaac cgtccaaacc cacgcccgaa gacattttgg aacacaatgc
     9961 cgccggcggc gatgcgggcg taccccaagc cgcagaaggc gcgcccgaac cggaaatcct
    10021 gcatcctgac gacggcgagc gtgccgatac cgttaccgta tcacggggcg aagtggaaga
    10081 ggcgcgcgta caaaaccagc gtgcggaatc cgaaattacc aaactttggg gaggactcga
    10141 taccgacgtg caaaaagagt tggtcggcga acaacgcaag tgggcgcagg aaaaaatcag
    10201 caactgccga caagccgccg cgcaggcaga ccggcaggaa tacgccgaat acctcaagct
    10261 gcaatgcgac acgcggatga cgcgcgaacg gatacagtat cttcgcggct attccatcga
    10321 ttaggggcaa accgatgact accgtcccca aaagcaggat tcccgtcaaa ccgctgcccg
    10381 aaaaaaccac agacgaagcc aaagtcgaaa aatggcggca gctcggtgcg gaacacggtt
    10441 tgtcgggcga atgggcagtt gccgccagat tgggcgagaa cggttttacc gaagaacaga
    10501 tggaaaatat cgccaacctg ttcggcagat aaagggaaaa ttgacggaaa tgccgtctga
    10561 aaccctgtta tcggtttcag acggcatttt gactaatacg gtacgcaggc gcaaaacagc
    10621 cgtcttttcc tgcgtttcct atgctgatgt ttcaacacac aggacgatac aaaaagcgtc
    10681 gccctatgtt ccgtcctaat ttttgttaat ccgctctaaa gtctgatcct gccgccctaa
    10741 agggtgggat ttcaaccgaa aaggaaatac gatagaatcg gcaatttgcc gtaaagttaa
    10801 aaagttccat catatcg
//
LOCUS       DAXHMX010000066        10785 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHMX010000066 DAXHMX010000000
VERSION     DAXHMX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.066"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(163..1209)
                     /gene="adhP"
                     /locus_tag="ACFM4S_001213"
     CDS             complement(163..1209)
                     /gene="adhP"
                     /locus_tag="ACFM4S_001213"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6050895.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1492..1881)
                     /locus_tag="ACFM4S_001214"
     CDS             complement(1492..1881)
                     /locus_tag="ACFM4S_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6050896.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFDITLSEVSPDHFTLQADPNPTTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2107..3285
                     /locus_tag="ACFM4S_001215"
     CDS             2107..3285
                     /locus_tag="ACFM4S_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6050897.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITEAVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNSDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3351..5285
                     /locus_tag="ACFM4S_001216"
     CDS             3351..5285
                     /locus_tag="ACFM4S_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6050898.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5603..6385)
                     /locus_tag="ACFM4S_001217"
     CDS             complement(5603..6385)
                     /locus_tag="ACFM4S_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6050899.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6524..8713
                     /locus_tag="ACFM4S_001218"
     CDS             6524..8713
                     /locus_tag="ACFM4S_001218"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6050900.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPQGMRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     VKLIENWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKVQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRVRQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDSKIRWSVDVDPQEA"
     gene            complement(8759..9833)
                     /locus_tag="ACFM4S_001219"
                     /pseudo
     CDS             complement(8759..9833)
                     /locus_tag="ACFM4S_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(9881..10771)
                     /locus_tag="ACFM4S_001220"
     CDS             complement(9881..10771)
                     /locus_tag="ACFM4S_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6050901.1"
                     /translation="MFGFRLSGGKGILQRSQVALVVTESTGGLEIPAAKAIRRAGIAV
                     IIANPRQTHQFAQSQPLTKTDAKDAKMPAFFTQMMTQKEDWQTMPYQPPTEAEEVLEA
                     LVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDDHTHTHFDG
                     KAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLVGIAPHPRESGETKFKSRCFG
                     GRSAVLKALYMATVAATRFEPLIRDFYQRLLSEGKPYKVAVTVCMRKLLTISNARMRD
                     YFAENDTAENDTAENGIRTA"
BASE COUNT         2491 a         2798 c         3106 g         2390 t
ORIGIN      
        1 ccgccttgtc ctgatttttg ttaatccact ataatgacgt gttgcgataa gggttttcag
       61 acggtatttc aaacagaaca gattaaaatc aaatccaaat ccataaaaaa tgccgtctga
      121 acagcgttca gacggcattt cgatgtgtat cgccacgtca aatcagtggt ggtggccgca
      181 gccgcattct tttttcatat cgattaccat acgaccggtg attttgcctt cgcgcatttc
      241 ttggaaaatg gcgggtgctt catccaaagc acgcagttgg actttcggca caaccaaacc
      301 ttccgcgccg aattggaagg cttcttccaa atctttgcgc gtgccgacca aagagccgac
      361 cacttcgatg ccgtccaaaa ccaaacgcgg gatggacaaa tccatcgatt ccggcggcag
      421 tccgatggca accacacgtc cgcccgcgcg gacgcaattc acggcagagt tgaatgcggc
      481 agcagatacg gcggttacga ccgcagcgtg tgcgccgcct gttttttcct gaatcacttt
      541 ggcagcgtcc tctttggcgg cgttgacaac caaatccgcg ccggtttctt tggcaaacgc
      601 cagtttgtcg tcgttgatgt cgatggcgac aacgtgcgcg ccgaatactt ttttcgcgta
      661 ttggaccccc aagttgccca aaccgcccgc gccgtagatg gcaatccact gtcccggacg
      721 aacactggaa actttaatgg ctttataagt ggtcacgccg gcacaagtaa tgctcgaagc
      781 ttgcgcagga tccaaacctt cagggacttt gaccgcgtaa tcggcactca cgatgcagta
      841 ggtcgccata ccgccgtcgg cagtataacc tgcgttcaat acagagcggc acaaggtttc
      901 gcggccggta ttgcagtatt cgcaagaacc gcagctttgg aacagccagg cgatgctgac
      961 gcggtcgccg actttcagat ttttcacacc gtcggcaact tctttaacca aaccgatgcc
     1021 ctcgtgtccc agcacgcggc ccggtttttc gccgtagtcg cctgccgcaa cgtgcaggtc
     1081 ggtgtggcac acgccgcaat attcgacttc gaccaacgcc tcgccgtatt ccaacgggcg
     1141 aacctcgcgt tcgactattt ccacatcgcc cgctacattt ttattcacaa caactgcctg
     1201 cattttcatg attgcgctcc ttagttacgg caaaaaaacc tgtaaacgga atgttgtccg
     1261 atataagggt aagcatacgc ttccgcatct cacaggtcaa gtggtatgtt gttgaaaaat
     1321 atagattata tgttatatta taacatctcg gaaaggcacg gcatcggggc ggttgccgga
     1381 tgaggggcgg caggtttcaa gtttgaaaaa ccggacggca aacccgtaaa gataccgtct
     1441 gaagctgtgt ccggacggca tctttacggg tttgcgggct tcggcggagg attagtcgaa
     1501 gccggggcag gattggtttg taccggaagc ggcaatggta ccgccgtcgt tgagcgtaac
     1561 gacgcaggtt tcgccgtcgt tggtggtggg attggggtcg gcctgaaggg taaagtggtc
     1621 ggggctgact tcgcttaaag tgatatcgaa atattcgttt tgtttcagtt tgtttttgtc
     1681 gtaggtttta aacgtccctt tttggcggta gtaacgttcc atggtctgcg cgttgtgcag
     1741 cagggtcgtc ctgacttccg acaggcggac gcgccggatg taggttttat aggaagggta
     1801 ggtgatgagc gtcaggatgc cgaggatggc gacggcaatc atcagctcga gcagcgtaaa
     1861 gcctttttga acgtttttca tagcaatgtg tttccatttg tttgtcggtc gacggcatta
     1921 taacgccgta tcggaaatgg cggaatatgt aaacggattg aaattttcgg gaaagcagat
     1981 tgtataagcc atttaaaaca aatggttatt tttattgtcg gcagtttgcc gccttggatg
     2041 gggcagggac ttgcggtaga atccgcttcc gatttatggg attgacgcat acagagaatt
     2101 gaaaacatgg caaaaatgat gaaatgggcg gctgttgcgg cggtcgcggc ggcagcggtt
     2161 tggggcggat ggtcttatct gaagcccgag ccgcaggctg cttatattac ggaagcggtc
     2221 aggcgcggcg acatcagccg gacggtttct gcaacagggg agatttcgcc gtccaacctg
     2281 gtatcggtcg gcgcgcaggc atcggggcag attaagaaac tttatgtcaa actcgggcaa
     2341 caggttaaaa agggcgattt gattgcggaa atcaattcga cctcgcagac caatacgctc
     2401 aatacggaaa aatccaagtt ggaaacgtat caggcgaagc tggtgtcggc acagattgca
     2461 ttgggcagcg cggagaagaa atataagcgt caggcggcgt tatggaagga aaacgcgact
     2521 tccaaagagg atttggaaag cgcgcaggat gcgtttgccg ccgccaaagc caatgttgcc
     2581 gagctgaagg ctctaatcag acagagcaaa atttccatca ataccgccga gtcggaattg
     2641 ggctacacgc gcattaccgc aacgatggac ggcacggtgg tagcgattct cgtggaagag
     2701 gggcagactg tgaacgcggc gcagtctacg ccgacgattg tccaattggc gaatctggat
     2761 atgatgttga acaaaatgca gattgccgag ggcgatatta ccaaggtgaa ggcggggcag
     2821 gatatttcgt ttacgatttt gtccgaaccg gatacgccga ttaaggcgaa gctcgacagc
     2881 gtcgaccccg ggctgaccac gatgtcgtcg ggcggctaca acagcagtac ggatacggct
     2941 tccaatgcgg tctactatta tgcccgttcg tttgtgccga attcggacgg caaactcgcc
     3001 acggggatga cgacgcagaa tacggttgaa atcgacggcg tgaaaaatgt gctgattatt
     3061 ccgtcgctga ccgtgaaaaa tcgcggcggc aaggcgtttg tgcgcgtgtt gggtgcggat
     3121 ggcaaggcgg cggaacgcga aatccggacc ggtatgagag acagtatgaa taccgaagta
     3181 aaaagcgggt tgaaagaggg ggataaagtg gtcatctccg aaataaccgc cgccgagcag
     3241 caggaaagcg gcgaacgcgc cctaggcggc ccgccgcgcc gataaacgaa tatgccgtct
     3301 gaacacggaa acggtttcag acggcatttg ttattgattt acggaatatt atgagcttga
     3361 tcgaatgtaa aaacatcaac cgctatttcg gcagcggcga gaaccgcgtc catattttga
     3421 aagacatcag cctgtcgata gagaagggcg attttgtcgc catcatcggg cagtccggtt
     3481 cgggcaagtc cacgctgatg aacatactcg gctgtttgga taccgccggt tccggttcgt
     3541 accgaatcga cggcatcgaa actgccaaaa tgcagcctga cgagctggcg gcattgcgcc
     3601 gcgaacgctt cggtttcatc ttccaacgct acaacctctt aagctcgctg accgcaaggg
     3661 acaacgtcgc gctgccagcc gtgtatatgg gcgcgggcgg caaagagcgt tccgcgcgtg
     3721 cggagaagtt gttgcaggat ttgggtttgg caagcaaaga gggcaacaag cccggcgaac
     3781 tctcgggcgg acagcagcag cgcgtctcca tcgcccgcgc cctgatgaac ggcggagaaa
     3841 tcatcttcgc cgacgagccg accggcgcgc tcgataccgc cagcggcaaa aacgtgatgg
     3901 aaatcatcca taagctgcac gaagccgggc ataccgtcat tatggtcacg cacgaccccg
     3961 gcatcgccgc caatgccaac cgcgtcatcg aaatccggga cggcgaaatc atttccgaca
     4021 cctcgaaaaa tccagaaatc cccgcaagca atgtcgggag gattcaggaa aaagcctcgt
     4081 ggtcgtttta ttacgaccag tttgtcgaag ccttcagaat gtcggtgcaa gcagtattgg
     4141 cgcacaaaat gcgttcgctt ctgacgatgc tcggcatcat catcggtatc gcttcggttg
     4201 tctccgtcgt cgcattgggc aatggttcgc agaaaaaaat ccttgaagac atcagttcga
     4261 tagggacgaa caccatcagc atcttcccgg ggcgcggctt cggcgacagg cgcagcggca
     4321 ggattaaaac cctgaccata gacgacgcaa aaatcatcgc caaacaaagc tacgttgctt
     4381 ccgccacgcc catgacttcg agcggcggca cgctgaccta ccgcaatacc gacctgaccg
     4441 cttctttgta cggcgtgggc gaacaatatt tcgacgtgcg cggactgaag ctggaaacgg
     4501 ggcggctgtt tgacgaaaac gatgtgaaag aagacgcgca ggtcgtcgtc atcgaccaaa
     4561 atgtcaaaga caaactcttt gcggactcgg atccgttggg taaaaccatt ttgttcagga
     4621 aacgcccctt gaccgtcatc ggcgtgatga aaaaagacga aaacgctttc ggcaattccg
     4681 acgtgctgat gctttggtcg ccctatacga cggtgatgca ccaaatcaca ggcgagagcc
     4741 acaccaactc catcaccgtc aaaatcaaag acaatgccaa tacccaggtt gccgaaaaag
     4801 ggctgaccga tctgctcaaa gcgcggcacg gcacggaaga tttcttcatg aacaacagcg
     4861 acagcatcag gcagatagtc gaaagcacca ccggtacgat gaagctgctg atttcctcca
     4921 tcgccctgat ttcattggta gtcggcggca tcggcgtgat gaacatcatg ctggtgtccg
     4981 ttaccgagcg caccaaagaa atcggcatac ggatggcaat cggcgcgcgg cgcggcaata
     5041 ttttgcagca gtttttgatt gaggcggtgt taatctgcgt catcggcggt ttggtcggcg
     5101 tgggtttgtc cgccgccgtc agcctcgtgt tcaatcattt tgtaaccgac ttcccgatgg
     5161 acatttccgc catgtccgtc atcggcgcgg tcgcctgttc gaccggaatc ggcatcgcgt
     5221 tcggctttat gcctgccaac aaggcggcca aactcaatcc gatagacgca ttggcgcagg
     5281 attgaggttg gacaaagatg ccgcctgaag ctgcagaacc ggtcattttg gagcagaaac
     5341 ttattggaca aaaacggttt cttagattct acgttccaaa ttcccacttg cgtgggaatg
     5401 acggcggtgt gtttcgtatt gttcaattta ttattttcaa tcattcaatg ggttaggatg
     5461 tgtttgttgg cttgctgtac tggtttaaat ttaatccact ataaatttaa tccactataa
     5521 ctttcagtgc ggattggttt tcaggcggca tttcccgcaa aaaaggcttg gaattttcca
     5581 agccttttgt tttgcggatg gattattgat ttttgcggat gatttcctcc agttggggca
     5641 tcgggctgta gccgctttgg ctgcgcccgt tggggaagac gagggtcggc gtgccgttga
     5701 agccgaattg ttcgcccaag gaagtggttt ccgcgacggg attgtcgcag atgctgccgc
     5761 cgaccgggaa tttgccttta cgcatccaat ccgtccacgc tttggcgcgg tcgggctgac
     5821 accataagat ttgcgccttg cgcgcggcat cggggtgcag gccggcaatg ggcatcataa
     5881 agctgtaaac cgtcacgtcg gtcatttttt caaactcgtg ttccaagcgt ttgcagaacg
     5941 gacaatcggg gtcggagaag acggcgactt tcagcttgcc gttgccgcgc acttctttga
     6001 tggctttgtc caaaggcagg gaggcgaagt cgattttgtt caaatcggcg gcgcgttctt
     6061 cggtcaggtt tttgcgcgtg tcgatgttga tgagttcgcc gacgaacata tagccgcctt
     6121 cggcatcggt gtagataatc tgtctgccgc tgacgacgac ttcgtaaatg cctttgaccg
     6181 gtgtttcgct aacgctcaac actttcaaat cttgggcgga ataggttttt tccaaacgcg
     6241 ctttcaaaga ggcggcaacg gatttgccgg cggactcggc tttggcggcg ggttcggcgt
     6301 tggcattgga aacgggcgtt tgcccgcaag ccagcagcgg gaggacggta aagggggtca
     6361 agattttgat taacttggtt ttcatataaa gatgattgcg cgtgttggaa aagcggaatt
     6421 gtatcaaatc tctgttgcgc ctgcattgcg cctaggctca atttatcgtc tgaaaatagc
     6481 ttccgcctgt taaaatacgc aaaaaaatga tttgcctgcc tgtatgatct accaccgtat
     6541 cgccgtaaac gtgccgcttt cagacggtct tttaacttat tcccattccg agccgctgcc
     6601 gcagggaatg cgggtgcttg tgccttttcg caataaaacc gtggtcggga tggtgtggga
     6661 aacggatatt gcgccggata tggatacggc gcggattttg agtattcaga cgaccttttt
     6721 agacgagccg ccgttgcctg aaagctggcg tgatttgctc tcgtttacgt cgcgttatta
     6781 ccactatccg acggggcagg cggtgtttgc cgcgctgccg cagggtttga aggaaacgcg
     6841 cgcggtggaa atgccgcagc cgccgttgtt ttacgcgctg aacgaagcgg gcagggcgca
     6901 aacgccgccg ccggcgcggt tcaacaaaaa agcggctttg tgggacgcgc tgctgtcggg
     6961 cgaaatgacg atggcagcgt tgaagcaggt aaacgcgcag gcggtgaaat taatcgaaaa
     7021 ttgggcggaa cagggttgga ttgaaacgac ggaagcggcg aagcccgttt taaggtcgtg
     7081 taatgggcag gcttcgcact ctgaatttgt attaaatgct gaccagcaga aggcttccga
     7141 tgccattcag acggcattgg gacgcttcca gccgtttctg ctgtacggca tcaccggcag
     7201 cggcaagacc gaggtgtatt ttgatgcgat ggcgaaagtg ttggctcagg ggcggcaggt
     7261 gttgtttctg ttgcccgaaa tcaacctcac gccgcagctt ttgaagcggg tagaaaaccg
     7321 ttttgccgac gtgccgaccg ccgtgttgca cagccagatg gcggcaggca ggcgcacgca
     7381 agattatttg cgcgcgatgt tggggcaggc gaaattagtc atcggcacgc ggctggcggt
     7441 gttcacgccg atggatgatg tcgggctgat tgtggtcgat gaggaacacg acggctcgtt
     7501 caaacaagac aacgaattac gctaccacgc ccgcgatttg gcggtgtggc gggcgaagca
     7561 gagcggctgc cccgtcgtgc tgggtagcgc cacgcccagc ttggagagct ggcacaaggt
     7621 gcaaagcggc gcgtaccgcc tgctgcaact gaccgagcgc gcccataccg ctgcgcaact
     7681 gccgcaagtg gacatcctca acgtaggccg tctgaaacta gacaatggct tctcgccgca
     7741 agccttgcag cttttgaaac agaattttga agcgggcggt atgtcgctgg tgtacctcaa
     7801 ccgtcgcggc ttcgcgcccg cgctgttttg cggcgactgc ggccatacct tcggctgccc
     7861 gaactgctcc gccaaaatgg tgctgcacca acgcgtccgc caactgcgct gccaccactg
     7921 cgaccaccgc gaacccatac cgttcaaatg ccccgactgc ggaaatcaag acctgaccgc
     7981 cgtcggacac ggcacgcagc gcgtcgaaga aaccctgcgc gccttcctgc ccaaggcagc
     8041 cgtcgtccgc gtggacaggg acagcaccgc gcacaaaaac gactgggcgg atttgtaccg
     8101 ccgcatcgcc gacaacgaaa tcgacattct ggtcggcacg cagatgctcg ccaaaggcca
     8161 tgactttgcg cggctcaacc tcgttatcgt attgaacgcc gacggcagcc tttacagcgc
     8221 ggatttccgc gcgccggaaa gactgttcgc cgagctgatg caggtgtccg gcagggcggg
     8281 gcgcgccgac aaacccggca aggtgttgat acagacccaa ctgcccgaac atcccgtctt
     8341 cgccgccgtc aaagcgcagg attatgccgt gtttgccgaa aacgaattga acgaacggca
     8401 aatgttcgcc atgccgccct tcggttttca gaccgccgtc cgcgcggacg cgccgcgtgt
     8461 tgccgatgcg ctggaatttc tcaatgccgc caaagaaacc ctcgccccgc tgttgcccgc
     8521 aagcgtttcc cagtttggcg ccgcccccat gctgatggtg cgcctcgccg aacgcgaacg
     8581 cgcgcaaatc ttcctcgaat ccccgtcccg acaggatttg caccgtgccg tgagtttgtg
     8641 ggtgcaggtg ttgcagcaaa accgcgacag caaaatcaga tggtcggtgg atgtcgatcc
     8701 gcaggaggct tgattatcgg caatacgatg ccgtctgaaa ccgtttcaga cggcattttt
     8761 attccgggtc gcccgtaaac gcattcgccc gaaaaatcgg aacaaacgtg aaaagataaa
     8821 gcgtgaacac gacggcggtc agaatcgcgg ggacggtgat gaagaatatc gggttcacgt
     8881 tcattaaaac agcgcgcgaa acggcggcgg cgaaaagaat ggggatggcg atgcggcaga
     8941 gtttggggta gtcaagtttg gtaaagccgc tgtgccacag tccggcagtc agccacacca
     9001 tcatcacgcc gcccatcatg ccgccgagag taatcaggtg caggggcgcg gaggagggca
     9061 gattttgcag tttcgccgcg cctgtccaca gatagcctgc ggcggcaaag agttggagta
     9121 ggtaataggt gcggacgtag tgtttgcgca ggagttcgtg atggtgcagt tcgcgcagct
     9181 tggcgagcag gatgaaaccg acggcgaatg cggtaaaacc ggcggtttgc gcgggcagcc
     9241 aaagttcggc ggcggcgtgc agcagcagga aggtgatggc gatgttttta tagacgacgt
     9301 tggggatgaa tacggggtct ttcagacggc attctttcaa cgcttccgca cccagcaaaa
     9361 cactcacgcg tatggaaaca aacatcaccg ccgccatatt caaatgcact tgcgcgcgca
     9421 gtaagttcaa atcgccgctg acggcatagg ccgtctgaaa aacggtaaat gcggcaagca
     9481 acgtcaaaag agcgaagttg tcggtgttgc ggtcgagcca aatcagccgg gcgcagaaca
     9541 gcagcaatac cagccaatag gcggcaacga aaaacgaggc agtttgcggc gaaaagggca
     9601 gtatagcgga tgcggcgagc aataatgccg ccatcaaagt cgcgacaggt ttcaggttac
     9661 ccgaaaaaaa ccgtccagtc caacaaagcc gcagtcagga aaccaccata tgccgcaggt
     9721 agcatgagtt ctaagaagat ttggcggtgc aggacgacgg caccggggct gatgaaaaac
     9781 accaacgcgc cgagtatggc aagcactgtc gcgccgacga aaaacgggcg catagcaact
     9841 gtatttttca ccccgtcggg caaaaatacc aaaactcaaa tcaagccgtc cggataccgt
     9901 tttcggcggt atcgttttcg gcggtatcgt tttcggcaaa ataatcacgc atccgggcat
     9961 tcgatatcgt cagcagttta cgcatacata ccgtaacggc aaccttatac ggcttaccct
    10021 cggacagcag gcgttggtag aaatcccgaa taagcggttc aaaacgtgtc gctgccacgg
    10081 tagccatata cagtgcctta agcaccgcag accttccgcc aaagcagcgg cttttgaatt
    10141 tggtttcccc gctctccctc gggtgcgggg caatgccgac caaactcgct atccgtttgt
    10201 gcgacagccg ccccaactca ggcagcatcg ccatcagcgt agccgtcgtt atcgaaccga
    10261 tgcctttgat ttgttctgcc acttgggctt tgccgtcaaa atgcgtgtgg gtgtggtcgt
    10321 cgatttgttt gtccaattcg tcaatcagcc ggtcaaaatg ggcaatcagt tgtttgacgc
    10381 ttccgacttg cgtttcgtga acctgatgca gacggttttt ctcggcagtc cgcatatccg
    10441 ccgattggtt gcggcggtta accaaggctt ccaacacttc ttccgcttct gtgggcggtt
    10501 ggtagggcat ggtttgccaa tcttctttct gcgtcatcat ctgtgtgaag aaggcgggca
    10561 ttttggcatc tttggcgtcg gttttggtca gcggctgcga ttgggcaaac tgatgcgtct
    10621 gacgcgggtt ggcgataatc acggctatgc ctgctcggcg gatggctttg gcggcgggga
    10681 tttcgagacc gccagtactt tccgtcacga cgagggcgac ctgagacctt tgcaaaattc
    10741 ctttccctcc cgacagccga aacccaaaca caggttttcg gctgt
//
LOCUS       DAXHMX010000067        10568 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHMX010000067 DAXHMX010000000
VERSION     DAXHMX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.067"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(214..1278)
                     /locus_tag="ACFM4S_001221"
     CDS             complement(214..1278)
                     /locus_tag="ACFM4S_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6050902.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEDIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVANRFTINHIADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="ACFM4S_001222"
     CDS             complement(1279..2037)
                     /locus_tag="ACFM4S_001222"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6050903.1"
                     /translation="MKKVSVLIVARNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="ACFM4S_001223"
     tRNA            complement(2112..2187)
                     /locus_tag="ACFM4S_001223"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3350)
                     /locus_tag="ACFM4S_001224"
     CDS             complement(2241..3350)
                     /locus_tag="ACFM4S_001224"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC6050904.1"
                     /translation="MEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVDAAEQALTDA
                     GLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVRMMPHTAAAN
                     IGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFSSEVYVFDS
                     LYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGA
                     NSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHNDSMESRAVAA
                     VFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPQLWDGQNDPNLP
                     AINLTGSGSRWETEKRIAASSSFTFGGSNCVLIIG"
     gene            complement(3354..3873)
                     /locus_tag="ACFM4S_001225"
                     /pseudo
     CDS             complement(3354..3873)
                     /locus_tag="ACFM4S_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            3974..4474
                     /locus_tag="ACFM4S_001226"
                     /pseudo
     CDS             3974..4474
                     /locus_tag="ACFM4S_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            4477..4560
                     /locus_tag="ACFM4S_001227"
                     /pseudo
     CDS             4477..4560
                     /locus_tag="ACFM4S_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            4841..5275
                     /locus_tag="ACFM4S_001228"
     CDS             4841..5275
                     /locus_tag="ACFM4S_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050905.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRRVTQVWCVFFIINGTL
                     AALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            5282..5797
                     /locus_tag="ACFM4S_001229"
     CDS             5282..5797
                     /locus_tag="ACFM4S_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6050906.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYESDTFGMHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            5817..7295
                     /gene="ubiD"
                     /locus_tag="ACFM4S_001230"
     CDS             5817..7295
                     /gene="ubiD"
                     /locus_tag="ACFM4S_001230"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6050907.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(7359..9473)
                     /locus_tag="ACFM4S_001231"
     CDS             complement(7359..9473)
                     /locus_tag="ACFM4S_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6050908.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            9564..10457
                     /locus_tag="ACFM4S_001232"
     CDS             9564..10457
                     /locus_tag="ACFM4S_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC6050909.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
BASE COUNT         2323 a         2590 c         3040 g         2615 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc ggcttatatt gcatccaata atttgtcggc
      241 gatatggttg atggtaaaac ggttggcgac gcattgatgc cctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcatcc gccaaagatt caggtgtcag
      361 cctgtccagc agaatccccg attgatgatg ttcgataatt tccttttgcg cgcccaaagt
      421 attggaaatc actgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgtcttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatccggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcagt atcacacagg cttcaatcag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaacg
      781 gccggtatcg ataccgttat gaacaatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtgataa aaatcggttt tgttggcgac gacattgtgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggtttttcc gaataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat cgtgcagccc cgcagtccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccgcc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccggatttg tacagataat atagtttgac
     1321 atatttaatc attgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 gagaaaccct ttgttcagga tataaatttt gaaaaacccc caaattgggc ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg aaatggaagt
     1501 atatttgttg aatttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttag ggtagggggt ctgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgcct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ttcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc gcccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcatcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt catttctagc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggta
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gaaggttcgg atcgttctgc ccgtcccaaa gctggggcgg aagttttcct
     2401 tcgggattgc tttgccggtc ggcaatgccc cacgcgaacg cggcttcgat tgcgcccgcc
     2461 gcacccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcacggct ttccatactg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttgcgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattatc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgagaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagtccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacgtgcc ggtcagcaac
     3121 aattcgccca catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atccgtccgt cggtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatc ctcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccactat attttcgcaa gacgcgtttt gcctgattcc aaaagttctc
     3421 aatgccgtta atgtggttct gacggtctgc aaattccttg gaatggttga tgcggtaatg
     3481 gataaaaccg ctcacgtcca acttgtcgca gctgctcaga ctatcggtat aaacaatact
     3541 gtccggcatg attttctttt tgatgacagg gagtaacgtt tcagacttgg cattatccac
     3601 cacaacggta tagacccgtc cgttgcgttt cagaatgccg aagacaacca cttttcctgc
     3661 cgcaccgcga ccacgtctgc ctttacgccg tccgccgaaa tcgctttcgt ccggctcgac
     3721 agagccctca aaaacctcat cggcagccaa ggccaaatga tggttgataa ccatgcggat
     3781 tttacggtag aacagtgctg ccgaattggg atggataccc aaaatatcgg cggcagaacg
     3841 ggcggtaact tcgagtacaa aaaaacggag cagttctttc tgtacttttt tctttcattt
     3901 gcagtgcgtt atcttcatat ttcgagggta acatatctgc taatctagta cagcccctaa
     3961 aatttaatcc actatagttt atgcctcgca cgacggggaa atggcgcgca gtttcgattt
     4021 gtggttggaa ctgttgaaat cgcaaatcgt gtcgccgact tcgttcggtt tgtcggtgca
     4081 caatgcaacg gcggggtaat ggtcgatatt gaggcgggat atgagcgagc agacggcatt
     4141 ggccgtgtgt gccgacggtg tggaaacggc attggcggaa gcggcatcac tgttggaaga
     4201 gggatgcggg tcggttttgg ttttggcggc ggacgatccg ctgccggaag gatatgccgt
     4261 atcggcaacg cgcgcgccgt ttgcttatgc tttggcgatg gttctgacca aagggacgcg
     4321 ttacagtctg actttatctg cttcggacga tatgccgtct gaagccggta tgctgcccga
     4381 ggcatattgg agcggtttgg aatgggtgcg ctttctgctg aacggcagtc gggaatgccg
     4441 ccgggtatat cgcaatcgtg aatggctgtg gcagcccgca agctgcaggc tgaagatttc
     4501 cgcaatgtcc gcaccgtcaa cgacgtggta caggcggtat tgaagattca ggcgggctaa
     4561 tcgggtattt ctaattattt ttcagacggc atgtttttat cggatgccgt ctgaaaacat
     4621 ttgtgcggat tcaaaatgaa acccggtttt ttcggacata tttttctgac cgtattaagc
     4681 attgcctatc ccgctttgtg gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     4741 cgcagacagt acggcaaggc gaggcaacgc tgtactggtt tttgttaatc cactatatta
     4801 cggcagggag gcgggctggt ttgtctggct ggcggcggcg atgtgcgggt tgtggctgct
     4861 gcgcgccctg actgccaaac tgccccaaca gcgttatgcg gctttgttta ttacactgtt
     4921 ttttgccgca gtattggtgt tcgggcggca tgattcgatg tattggtatc ccgtcctcgt
     4981 caatctgatg atgttggcgg tgttcggcgg cagcctgttt gcagggcaga cggtgattga
     5041 aaaacttgcg cggctgcaac agcccgacct gcctgaaaaa gccgtgcgct atacccgccg
     5101 ggtaacgcag gtttggtgcg tattttttat aatcaacggg acgcttgccg ctttgttggc
     5161 acggttgggg cgatatgatt ggtgggcggt ttatacaggc gtgattgcct atgtattgat
     5221 ggggatgctg tttgccggcg aatggcttta ccgcaaactc gttttgaaag tctgaaacct
     5281 tatgtacact tgcacttcct gcggcgaaaa acatgaagat atgcccgcaa tcggctttac
     5341 cgcccccgat ccctataatc aactcagtga agaagaacgc attgcttatc aagcagaatg
     5401 cgattctgat ttctgcatca tccgctatcc cgaccaaacc gaccgcttta tccgcgcggt
     5461 gctgccgatt ccgattatcg ggcatgagga aacgctggaa tatggcgtgt gggtgtccgt
     5521 cagcgaaaag agttttaacg actatcaaag ccactttcac gacaatccgg aaaatgtcgt
     5581 ttatttcggg atgatctgca accggctgcc cccttacgaa tccgacacct tcggtatgca
     5641 ttgcaatgtc gttacccaac ccgatggtca acgccctttg ctgcaactcc atcaaggcag
     5701 cgaacatccg ctggtacgcg acttttatca cggcatagaa tatgccgagg cacaggcaag
     5761 gataggggcg gtatccgaat cggattgctc aggataaaag gacaaaaagg atgaatatga
     5821 aatacaaaga cctgcgcgac ttcatcgcca tgctcgagca gcagggcaaa ctcaaacgca
     5881 tcgcgcaccc cgtttccccg catttggaaa tgaccgaaat cgccgaccgc gtgctgcgcg
     5941 ccgaagggcc ggcgttgctg tttgaaaacc cggttaagcc cgacggtacg cgctacggtt
     6001 atcctgtgtt ggcaaacctg ttcggcacgc ccgaacgtgt ggcgatgggc atgggcgcgg
     6061 acagcgtgtc caagctgcgt gaaatcgggc agacgctggc gtatttgaaa gaacccgaac
     6121 cgcccaaagg catcaaagat gcgttttcca aactgccgct actgaaagac atttggagca
     6181 tggcgccgaa cgtggtgaaa aacgcgccgt gtcaggaaat cgtgtgggaa ggcgaaaacg
     6241 ttgatttgta taaacttccg attcagcatt gctggccgga agacgttgcg ccgctggtaa
     6301 catggggctt gaccgtaacg cgcgggccgc acaaaaaacg ccaaaatctc ggcatttacc
     6361 gccaacaact catcggcaaa aacaagctga ttatgcgctg gctgtcgcat cgcggcggcg
     6421 cgttggatta tcaggaattc cgcaaactca atcccgatac gccgtatccc gtcgccgtcg
     6481 tgctcggctg cgaccctgcc accattttgg gcgcggtaac gcccgttccc gataccttga
     6541 gcgaatacca gtttgccgga ctgctgcgcg gttcgcggac ggagctggtg aaatgtatcg
     6601 gcaacgattt gcaagtgcct gcacgcgcag aaatcgtgct ggaaggtgtg attcatccga
     6661 acgaaaccgc gttggaaggc ccgtacggcg accacacggg ctattacaac gagcaggatt
     6721 atttccctgt gtttacggtc gaacgcatca ccatgcgcga aaacccgatt taccattcga
     6781 cctacacagg caaaccgccc gatgagcctg ccgttttggg cgtggcgttg aacgaagtgt
     6841 tcgtaccgct cttgcaaaag cagttccccg aaatcaccga tttttacctg ccgcccgaag
     6901 gctgctccta ccgcatggcg gtggtgagca tgaaaaaaca gtacgccgga cacgccaagc
     6961 gcgtgatgat gggctgctgg tcgttcctgc gccagtttat gtacaccaaa ttcatcatcg
     7021 tggtggatga cgatgtgaac gtgcgcgact ggaaagaagt catctgggcg gtaaccacgc
     7081 gcatggaccc cgtgcgcgac accgttttgg tggaaaacac gcccatcgac tatctcgact
     7141 tcgccagccc ggtcagcgga ctcggcggca aaatgggctt ggacgcaacc aacaaatggc
     7201 cgggagaaac cgaccgcgaa tggggacgcg tcatcaaaaa agaccctgcg gttacggcta
     7261 agattgatgg gatttgggag gaattggggt tgtaggtttt acggtctgcc gtctgaagcg
     7321 cgggggcgtt tcagacggca tttttgtacg caggatgttt acggttcttt aggtttgagc
     7381 cactgccatt gttccagcag ggtgtcttcg aggattttct gtttctcttt gcgcgaattg
     7441 atgccgccgg tcagcctgcc gtaatcgacg gtaatggacg gcttgtccac cgtaccggtg
     7501 attttcaggg gaatcggttt gtttttcgga tggacggcgt tgcggataag gacatcttca
     7561 gacaattcct gcgtatccag attggtatag ccgttgctgg taacatagag gctgtcggag
     7621 aagagttcgg tatcgatgtg gcggctgatg ccgtctgaaa tttcgctgtt gagcgtgaat
     7681 cggtggaagg gtgtgctggg tgcggcattg tcggcagttt tgcccgaaat gccgtttttc
     7741 aggatattgt ccatgtcgat accgtgccat gcaccgttgg aaatattcag cgacaggctg
     7801 ccctgaagcg agcggataag ctcttttcgg gtttcgccgc tcgcggtcag gtcgatgacc
     7861 gcatcgccgt tgccgctgaa gctgtggaag ccgaacaggt cttgcagcag cggttggatt
     7921 tggatgttgc ttgcattctg ttgcaggcgg taagtggcgg gacgggtgtt ggcgatgctg
     7981 atgccgcctt cggtatggcc gccgtaaagc cctgacttga aacggctgag cgcgatatgg
     8041 cctttgtcgg cgtggaggta ggtttccata tcgtccagtt gcaggccggg aagttggact
     8101 tttccgattt tcaggtgcgc ctcgatgtcg ccggacagct tggcgagggt gtcgggaaat
     8161 attttgccgt tttgttgccg cacgtcgtca agataggggg tcaggttcaa tttttgcagt
     8221 gcgacggcgg cttccagatg cggtgcgtct tcatgtgtgt atctgaattt cgcggcaacg
     8281 gtttggcggt cgaatgtgcc gtttaattcg gcattccaat tttgcagatt cggtacggac
     8341 agcgaaccgt cgagccggct gatgaaacgg ggttgcggca ggcggttgac ggtatcctga
     8401 agggtcgata catacaggcg cggcgcgtcc agccctttgt tttccgtcca tacgagcggc
     8461 gaattgaggg agaagttggt ctggtggcgc ggtgttttga agctgccgga gatttcggcg
     8521 ttgccgatgt tggcgatgcc ggagtgaagg ttggctttgt cgagtttgaa cgaaccgtcc
     8581 cattgggcat attcgccgcc ggcggtaaat gcgccgttga cggtttcaat tttaatgctg
     8641 ttatttctga gtgccagcgt agggatttgg gcggtcaggt ggaggttgcg gaaggaagtg
     8701 tctgcacgca ggccgaggcc ggcggcatcc gctccgccgg cgttgaagcg gacagaaggg
     8761 ctgccggtgg tggaaatggt aatgccgtgt ccgtccagcg aagtggaagc ttcaaaatgg
     8821 aacggtgaga ttttgggcgt gccgatgccg tctgaaagga acagccccct gcttttccac
     8881 gggacggaca gctttcccca aaccagtatg cccgaacttt caaacggctg ccccgacgaa
     8941 tcgggggatt gcaggttgag gttgatttcc ttcaggataa gctgttcctg caggaaattg
     9001 aggcggacgg tgctgttttc gacgataatg cggttgactg aggcttggcg tttttggctg
     9061 tcgatcaggt cttggatgtt ccaaacacct ttcccgtcgc gcgtcagggc aagttccgca
     9121 ctcgaaacca cccatttttc aatctgtatc tgatccgacc acaggttttt ccagctcaat
     9181 ccgattttgg tttcttggac ggaaacggca gtctggtcgc cgccgggttc ggtaatggtc
     9241 aggtttttca ggatgacggt cggccggggc aggagcctgc gctgaatgtc cgcatcaaac
     9301 gagattttcc ggtgtgtgtg tgcaatgctt tgttggaggc ggctgcggat gttttcaggc
     9361 gtgaaggtgc gatatacgga agcgtgcagc ccgactgccg ccaaaagcag tatcgtcagc
     9421 acggccaccg catatttcag acggtatttg tggaaaaccg ataataaatc catagcccga
     9481 acaatctgta aaacacgcca cagtataaca cggcttgcaa aaatcctatt ttgggcaggc
     9541 cgtttcttgt gtcaggagga aatatgatta ttgtcatgag caggcgtgcc gccgaagcgg
     9601 acattgccgg cgttgtcgcc tttatccgca gcagaggtct gcgagaacac atttctcacg
     9661 gcgacgagcg taccgtcatc ggcgcaatcg gcgacgatag ggttttgagc gtcagggagg
     9721 tgcagacgct gccggaggtc gagaaagccg tccgcatttt ggatacatgg aaaaccgtca
     9781 gtcgggaaaa tcgggcggaa gacagccgcg ttgccgccaa aggcgtggct ttcggcggcg
     9841 gggaaaccgt ctgtatcgcc gcagaaccgt cagtatggcg caatgcggat gcagtgtttc
     9901 tggatccgtt tttcacttcg gcaaacttat acgacacttc aagtgcggat gaagggcgag
     9961 gacggtgcag acggttggcg gaacagaccg catcggcgca tggcgcaggg aaacccgtat
    10021 tggtgcgcgt ccgcaacgtc cgccatgtcg aagcggcttt gaatgcgggg gcggacatcc
    10081 tgtatttggg cggcgggctg atgtccgatc ttgccgtatt gaatgaagcc ggaagcctga
    10141 acattccctt ggtgctgtgt aaagacaagc accattctgc cgaagattgg ttgaatgccg
    10201 cagaatatgt cgtctcgcgc ggcaaccgcc acctgattct gggcgagtcg ggcgtgttga
    10261 gccataccaa agaccatccc taccgtttgg atgtcgaatc catcgtgaaa gtccgccaaa
    10321 tcagccatct gcccgtgatt gcgaatatta cgggattatg gaaccgcgat atgccgcagg
    10381 aaatcctgta cggcttggca aaggcggcag gcgcgtgcgg aatcgtcgga acctgttttg
    10441 aaaagtcggg cggatagcag gcataccggc agattcaaag gcggagcaga cattcggaca
    10501 acatgccgtc tgccgcccga atagcggtgc cgtctgaagc gcacaagacg gtttcagacg
    10561 gcatataa
//
LOCUS       DAXHMX010000068        10491 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHMX010000068 DAXHMX010000000
VERSION     DAXHMX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.068"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..103
                     /locus_tag="ACFM4S_001233"
     CDS             <1..103
                     /locus_tag="ACFM4S_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6050910.1"
                     /translation="CKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            complement(136..1227)
                     /gene="mltB"
                     /locus_tag="ACFM4S_001234"
     CDS             complement(136..1227)
                     /gene="mltB"
                     /locus_tag="ACFM4S_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6050911.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDTAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRAVVDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1376..2803)
                     /locus_tag="ACFM4S_001235"
     CDS             complement(1376..2803)
                     /locus_tag="ACFM4S_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6050912.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            2988..6671
                     /gene="mfd"
                     /locus_tag="ACFM4S_001236"
     CDS             2988..6671
                     /gene="mfd"
                     /locus_tag="ACFM4S_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6050913.1"
                     /translation="MTYPIPKPREKSRWLNLSQGSLPLALARYLPHRRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDNEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQYLYLSADVFAGRLKNYGQVLP
                     DVSGKEYTLPDLAVNRQADEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPSQQQAAPASEGEGDAITDQTE
                     FSASATNPLPSPLPQEREQSTAAISDSLKTKSSLYPIENSLHGQIQQQPAPSPVGEGW
                     GEGKAVAAQSAIAVITESDLYQYVARSRIHNRRKKHAAVSDDLLRDLAEINIGDPVVH
                     EEHGIGRYMGLITMNLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHENIALH
                     KLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQAFADGFGYEE
                     TEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTT
                     LLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDD
                     IKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSL
                     ITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERLETLL
                     PEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKF
                     GLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLE
                     IRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSP
                     ALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLR
                     LMAKELGINAIDAAGEAVTVTFGKNNNIDPTEIILLIQNDKKYRLAGADKLRFTAEME
                     NIEVRINTVKNVLKTLQNRCLPK"
     gene            6820..7203
                     /gene="panD"
                     /locus_tag="ACFM4S_001237"
     CDS             6820..7203
                     /gene="panD"
                     /locus_tag="ACFM4S_001237"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6050914.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7225..8067
                     /gene="kdsA"
                     /locus_tag="ACFM4S_001238"
     CDS             7225..8067
                     /gene="kdsA"
                     /locus_tag="ACFM4S_001238"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6050915.1"
                     /translation="MDIKINDFTLGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8082..8522
                     /locus_tag="ACFM4S_001239"
     CDS             8082..8522
                     /locus_tag="ACFM4S_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6050916.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSALGGSFGMWHGISSIVFMAAALLSIYLSWRLYGKNAV"
     gene            8567..9853
                     /gene="eno"
                     /locus_tag="ACFM4S_001240"
     CDS             8567..9853
                     /gene="eno"
                     /locus_tag="ACFM4S_001240"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6050917.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYLGKGVLKAVDHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDAGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            9867..10145
                     /gene="ftsB"
                     /locus_tag="ACFM4S_001241"
     CDS             9867..10145
                     /gene="ftsB"
                     /locus_tag="ACFM4S_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6050918.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10186..10476)
                     /gene="yfaE"
                     /locus_tag="ACFM4S_001242"
     CDS             complement(10186..10476)
                     /gene="yfaE"
                     /locus_tag="ACFM4S_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6050919.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         2507 a         3048 c         2683 g         2253 t
ORIGIN      
        1 ttgcaaatct ttcccactgt tcttgaaaga atgtgaattt cgatttaact tcggcacacc
       61 gtctcaacag cttaaaatcc tgcgggattg gtgtgggatt tagggctaat ctagtacagc
      121 cccttttaaa aaacattaca atcccgggcc gccaagcgaa ttggcaatgt ccctgaccgc
      181 cgttacatac atccggctgt ggttgtactg ccataccgta taaaaattgt tcaagcccaa
      241 ataatattca aacacgcccg gtgcggtttc cagtttgaac aaaaccgcct tttcatcatc
      301 ggcaagctct tcgccgggga tgatgccgta cgccttcaaa tccgccaccg tccgcgtcag
      361 ggcggttttt tcgccaatga ttgcctgaac atccgcaccc ggcgccaatg ttgcagacac
      421 cagcatttta ccgcccgtgc gccaaccgtg ctgcttcata taattggcaa ccgatgccgc
      481 gacatcgccg acgttgcccc atatgtcccg atgtccgtcc ccgtcataat ccaccgccca
      541 tttccggtag ctcgaaggca taaattgcgg catacccatt gcacccgcat agctgccctt
      601 aaaggcgaaa acatcgccgc cctcttcttt tgccagcttt aaaagctcga ccaattcttt
      661 ttggaaaaac ccggcgcggc gggggtaatc gaaacctaaa gtcgccaatg cgtccgccac
      721 gcggaaactg cccgtatttt tgccgtaatt cgtttcaatc ccgataatcg ccacgataag
      781 ctcggcgggc acgccgtatt tttgcgccac atcatcgaca actgcgcggt tttccgcata
      841 aaaccggcgc gcgccgcgaa atttcgcctt gcccgaattt cccgtgcgga acacatacca
      901 cggacgcgat gtggagggtc ggtgcataat cttgacgatg tccgccttgt aagccgcttt
      961 gtcaaaaaaa tcctgccatt ccgcccggga aaaatcccct ttcccgactt catcgtccac
     1021 aaaacggcgg acatttgcat tggcggcaaa cccgctgtcg gataccggta cggctgccgt
     1081 gtcaaacgcg gggcggcttt cttttttcat ttcatccgcg cggggggctt gggcttcatt
     1141 tacccggggc gggcgtgcct ccatcgccgt acaggcagac aaagccgcca aacaaattgc
     1201 cagcggcagt atttttctct ttttcataaa tatgttccga acaaataggg taagtgggaa
     1261 agcggcgcaa ggggcgcggt gcgaaatgcg gcatccgccg caatcggcgg ctttgtggaa
     1321 taccgcgcgt tgccttttgc accgcctgaa atccgtatgc cgccgtattt gcccgctatc
     1381 cgcaatatcg gcagtccaat atatctttgc ggatgtcgtt cagcaggaag gctgcggtgt
     1441 cttcgtgttc cgcctgtacg aagacaaaga gtcgggcgat gattttgccg atgaagactt
     1501 tttgcagggc gcaggcgtcg gtcagggtgt gttcctgcag gaagctgcgg atgtcttcgg
     1561 gcaaagtgta gtcgcgggcg acggcggcaa agcgggcatc ggtttgcagg cggtcgagca
     1621 gggtttggtt tttgtccaac ttcatgcctg cttctttttc ttcggcggtg gcgcggacga
     1681 actggtcgta aatttcggca taaagcatat cgttcgggcc ttcaaaaatc gtgaaggggc
     1741 ggatgtcgat agcgatattg ccggcggtgt gtccgcgttc aaaacccttc gcgcccaaaa
     1801 gtttttgcaa catttgcgcg gcggcgtaag tgtattccgt ggcgagggtt ttgacgatgt
     1861 tcgcctccat cagctgatgg gcgacggggg caacaggtga aacggaatgg cagacgtagc
     1921 ggtaaagaat ctcggaaacc tgatggcggc gccggatttc gcggcgttcg taatcgacga
     1981 atctgatgtc gttgcggacg tatcgttcca ggttttcaag gatgtattcc ataatgccgt
     2041 gcgtcatgcc gatcagttgc aggcggctgc ggataaagat gttttggaac gcgcgcaaac
     2101 cggcagcgtc gctctgggag agtttcatca cggcggttgc aggcatttcg gcatcgatgc
     2161 ggttgacggc gtaacggacg gcgcgcaagc cttcggatgc gagggtttcg cagcggatgt
     2221 atgttttggg gacgagcagc aggtcgatga ctttggcgag tttgccgttt ttgcgctctt
     2281 tggcggcaac gaggaggaag tcgctttgcg agttgccctg ccagtatttc gcggcgttga
     2341 cgtaaatggt ttgtccgtcg gtatattcgt agtaagactg catttctcgg gcaatcgccg
     2401 cgccggaggt ttcgggttcg gtaacgccca aaccaccgcc ctcgcctttg aaaaccatgt
     2461 ccaaaccctg tgcgatttgc gcttcatcgc cgaactcttg cagtggctgc aacaccagcg
     2521 cgccttcgat gccggtacgc agcgtaacgg gcacgccgta atgccccgca atccgcaaga
     2581 cttcctgaat ttcaaactgg ctgcccttgc gcccgccgtg ttttttgtcg aggaagggca
     2641 acagcaaacc cgcctgcttc aaggcaagcc atttgtcttc gggcaggtat cgcatcaggt
     2701 cgataccgtc tgaaaaaatg cggcggaatg cggattcgat gtgctttaaa aaagcagccg
     2761 tgtccatagt tgacggctgc gcactcggtt cggtgtgtat catcggcttc ctctgtcggt
     2821 tcccattaat cggcggccgg tcaaaccgcc tgccacggtt tagagttaat tttctaaact
     2881 ttaccacaaa gtgcgccggg caacaatccg ccgacctttc agacggcatc gcgccccctc
     2941 ccgtgctaaa atgaccgttt gcatcactgt ccgccgattg ccgcactatg acctacccca
     3001 tccccaaacc ccgtgaaaaa tcccgttggc tcaatctgtc tcaaggctcg ctacccttgg
     3061 ctttggcgcg ttatctgccg cataggcggc tcaaagtcgt gctgacccaa gatgcggaac
     3121 aggcgctacg ccttcagacg gcgtggcggt ttttccgtcc gcacgatacg gcggtgttcc
     3181 tgccggactg ggaaacgctg ccttacgagc gtttttcgcc gcatcaggat ttggtgtcgg
     3241 agcgtttgtc ggcgttgtgg cagattaaaa gcggcgcggc ggacgtgttg ttcgtgccgg
     3301 ttgccacggc gatgcagaag ctgccgcccg tgccgtttct ggcggggcgc acgttttggc
     3361 tgaaaacggg acagactttg gatataggcc gtctgaaaag cgatttggtg gatgcaggtt
     3421 ataaccatgt ttcccacgtt gtcgcggcag gcgagtttgc cgtgcgcggc ggcattgtgg
     3481 atttgttccc gatgggcagc gaaatgccgt accgcatcga tttgttcgac gatgaaatcg
     3541 acagcatcaa aacctttgac accgagacgc agcgcaccat ctcccccgtt tccgaaatcc
     3601 gtctgctgcc ggcgcacgag ttccccaccg acaacgaggc gcaaaaaatc ttccgcagcc
     3661 gttttcgcga ggaagtcgat ggcaatccga acgatgccgc tgtgtacaaa gccgtcagca
     3721 acggtcattt cggcgcgggc gtggaatact acctgccgct gttttttgaa aacgagttgg
     3781 aaacgctgtt tgactatatc ggcgaagatg cgctgtttgt ctctttaggc gatgttcatg
     3841 ccgaggcaaa ccgtttttgg agcgatgtca aatcgcgtta cgcgatggcg cagggcgacg
     3901 aaacctatcc gcctttgctt ccacagtatt tgtatctctc tgccgatgtg ttcgcaggcc
     3961 gtctgaaaaa ctacggacag gtgctgcccg atgtttccgg caaggaatac accctgcccg
     4021 accttgccgt caaccgccaa gcagatgagc cgttgcaggc attgaaggat tttcagacgg
     4081 cgtttgacgg acggattttg ctgtgcgccg aaagtttggg acggcgcgaa actatgctcg
     4141 gtttcttgca gcaaaacggt ttgaaagcca aacccgtgtc cgactggcag ggctttttat
     4201 cggcacacga gccgctgatg attactgttg cgccgttggc atacgggttc aaactgggcg
     4261 ggctgcaatc tccgagccaa cagcaagctg ctcccgcctc cgaaggagaa ggcgatgcga
     4321 ttaccgacca aaccgaattt tccgcatccg caacaaaccc tctccctagc cctctcccac
     4381 aggagaggga acaaagtacc gccgccattt cagacagtct gaaaaccaaa agcagcctgt
     4441 accccatcga aaatagtctg cacgggcaaa tccaacagca acctgccccc tcccccgtgg
     4501 gggagggttg gggagagggc aaagcagttg ccgctcaaag tgccatcgcc gtcatcaccg
     4561 aatccgatct ctaccaatac gtcgcccgtt cgcgcatcca caaccgccgc aagaaacacg
     4621 ccgccgtttc agacgacctg ttgcgcgacc ttgccgaaat caacatcggc gaccccgtcg
     4681 tgcacgagga acacggcatc ggccgatata tgggcttgat aacaatgaac ttgggcggcg
     4741 aaaccaacga aatgatgttg ctcgaatatg cgggcgaagc gcagctttat gtgcctgttt
     4801 cgcaactgca tttaatcagc cgctactccg gtcaggcgca tgaaaacatt gccctgcaca
     4861 agctcggcag tggcgcgtgg aacaaggcga agcgcaaagc cgccgaaaag gcgcgcgata
     4921 ccgccgccga gttgcttaac ctttacgccc gacgtgccgc ccaatcggga cacaagtttg
     4981 aaatcaacga gttggattat caggcgtttg ccgacggctt cggctacgaa gaaaccgaag
     5041 accaggccgc cgccatcgcc gccgtgatta aagatttgac gcaggcgaag ccgatggacc
     5101 gcctcgtgtg cggcgatgtc ggtttcggca aaaccgaagt cgccctgcgc gccgcatttg
     5161 tggcggtgat gggtggcaaa caggtcgccg tactcgcccc gaccacgctt ctggtcgagc
     5221 agcacgcgca aaactttgcc gaccgtttcg ccgatttccc tgtgaaagtc gccagccttt
     5281 cgcgtttcaa caacagcaaa gccactaaag ccgcgctgga aggcatggcg gacggcacgg
     5341 tcgacatcgt tatcggcacg cacaaactgg tgcaggacga catcaaattc aaaaacttag
     5401 gtttagtgat tatcgacgaa gaacaccgct tcggtgtccg ccaaaaagag cagctcaaac
     5461 gcctgcgcgc caatgtggac atcctcacca tgaccgccac gccgattccg cgtactttaa
     5521 gtatggcgtt ggaaggactg cgcgacttct cgctgattac caccgcgccc agccgccgcc
     5581 tcgccgtcaa aacctttgtc aaacccttta gcgaaggcag cgtgcgcgaa gccgtgttgc
     5641 gcgaactcaa acgcggagga caggtatttt tcctgcacaa tgaagtagat acgattgaaa
     5701 atatgcgcga gcggctggaa accctgctgc ccgaagcccg catcggcgtg gcgcacggac
     5761 aactgcgcga gcgcgagctg gaacaagtca tgcgcgactt tttgcagcaa cgatttaacg
     5821 tgttgctctg ctccaccatc atcgaaaccg gcatcgacat ccccaacgcc aacaccatca
     5881 tcatcaaccg cgccgacaaa ttcgggctgg cgcaactgca ccagcttcgc gggcgcgtcg
     5941 gccgcagcca ccatcaagcc tacgcctacc tgctcacgcc cgaatacatc accaaagacg
     6001 cagaaaaacg cctcgacgcc atcgcggcgg cagacgaact cggcgcaggt ttcaccctcg
     6061 ccatgcagga tttggaaatc cgcggcgcag gcgaaatcct cggcgaaggg cagtccggtg
     6121 aaatgatgca ggtcggcttc acgctctaca ccgaaatgct caaacaggcc gtgcgcgacc
     6181 tcaaaaaagg ccgccaaccc gacctcgatg caccgttggg cattaccacc gaaatcaaac
     6241 tgcacagccc cgccctgctg cccgaaagct actgccccga catccacgaa cggctcgtcc
     6301 tctacaaacg cctcgccgtc tgcgaaaccg tacagcaaat caatgccata cacgaagaac
     6361 tcgtcgaccg cttcggactg cccgagcaac ccgtcaaaac ccttatcgaa agccaccact
     6421 tacggcttat ggcaaaagaa ttgggtatca atgccattga tgcggccggc gaagcggtaa
     6481 cggtaacctt tggcaaaaac aacaatattg atccaaccga aatcatcctg ctgattcaga
     6541 acgacaaaaa ataccgcctt gccggcgccg ataagctgcg gtttaccgca gagatggaaa
     6601 atatcgaggt cagaatcaac accgtaaaaa acgttttaaa aaccttgcaa aacagatgcc
     6661 tgcccaaata aagccgacac cgcaatgctg tctgaaacac cgttttcctt gtccgaaagc
     6721 cgccattatg aatttgaagg aaactccact ataatacggc attcagattt tcagacggca
     6781 tcgcgcccgt caaaccgcac acaaaccaaa aggaaataca tgttccgtac tatgcttggc
     6841 ggaaaaatcc accgcgccac cgttaccgaa gccgatttaa actatgtcgg cagcattacc
     6901 gtcgatcaag acctgttaga cgcggcaggc atctacccca acgaaaaagt cgccatcgtc
     6961 aacaacaaca acggcgaacg ttttgaaacc tataccattg cagggaaacg cggcagcggc
     7021 gtgatttgtc tgaacggtgc tgcggcaagg ctggtgcaga aaggcgatat cgtcatcatc
     7081 atgtcttacg tccaactctc cgaacccgaa atcgccgcgc atgaacccaa agtcgtcttg
     7141 gtagacggaa acaacaaaat ccgcgacatc atctcctatg agccgccgca caccgtgctg
     7201 taattccgca aacggacatc gattatggat attaaaatca acgacttcac cctcggcaac
     7261 aactcgccct tcgtcctatt cggcggcatc aacgttttgg aaagcttgga ttccaccctc
     7321 caaacctgtg cgcattacgt cgaagttacc cgaaaactcg gtattcccta tatctttaaa
     7381 gcctctttcg acaaggcaaa ccgttcctcc atccattctt atcgcggcgt aggcttggaa
     7441 gaaggcttaa agatttttga aaaagtcaaa gcagagttcg gcatccccgt cattaccgac
     7501 gtacacgaac cccatcagtg ccaacccgtc gccgaagtgt gcgatgtcat ccagcttccc
     7561 gcctttcttg cgcggcagac cgatttggtg gccgcaatgg cggaaacggg caatgtcatc
     7621 aacatcaaaa aacctcagtt cctcagcccc tctcaaatga aaaacattgt ggaaaaattc
     7681 cacgaagccg gcaacgggaa actgatttta tgcgaacgcg gcagcagctt cggctacgac
     7741 aacctcgttg tcgatatgct cggtttcggc gtgatgaaac agacttgcgg caacctgccg
     7801 gttattttcg acgttaccca ttccctgcaa acccgcgatg ctggttctgc cgcatccggc
     7861 ggtcgtcgcg cacaagcttt ggatttggca cttgcaggca tggcaacccg ccttgccggc
     7921 ctgttcctcg aatcgcaccc cgatccgaaa ctggcaaaat gcgacggccc cagcgcgctg
     7981 ccgctgcacc ttttagaaga ttttttaatc cgcatcaaag cattggacga tttaatcaaa
     8041 tcacaaccga ttttaacaat cgagtaacac ggtttcgcct tatgatgcag actttccgaa
     8101 aaatcagcct gtatgccgca accttatggc tcggtatgca gattatggcg ggttatatcg
     8161 tcgcgccggt gctgttcaaa atgctgccca aaatgcaggc gggcgaaatt gccggcgtat
     8221 tgttcgacat cctctcttgg agcgggcttg ccgtttgggg cgcggtactg gctgccgcct
     8281 ttgccgccct aacccggcgg caaaccgccc tgctgctttt tttattgtcc gcccttgccg
     8341 ccaaccgatt cttgattaca cccgttatcg aggcgctgaa atacgggcat gagaattggc
     8401 tgttgtcggc tttgggcgga tctttcggaa tgtggcacgg catttccagt attgttttta
     8461 tggcggccgc cctactttcg atttacttaa gttggcggct ctacggcaaa aatgccgtct
     8521 gaagcctacc gttttacctc acgtcacttt aatttggaga atatttatga gcgcaatcgt
     8581 tgatattttt gcccgcgaaa ttttggactc acgcggcaac ccaaccgtag aatgcgacgt
     8641 actgttggaa tccggcgtca tgggacgcgc agccgtacca agcggcgcat caaccggtca
     8701 gaaagaagct ttagaacttc gcgacggcga caaatcccgt tacttgggca aaggcgtatt
     8761 gaaagccgtt gaccacgtca acaaccaaat cgcgcaagcc ctcatcggta tcgatgccag
     8821 cgaacaatct tatatcgacc aaatcatgat cgaattggac ggtactgaaa acaaaggtaa
     8881 cttgggtgcc aacgcgactt tggccgtttc tatggccgta gcacgcgcag ccgctgaaga
     8941 tgcagggctg ccgctttacc gctacttggg cggcgcaggt ccgatgtccc tgcccgtacc
     9001 gatgatgaac gtcatcaacg gcggcgaaca cgccaacaac agcctgaaca tccaagagtt
     9061 tatgattatg cccgtcggcg caaaatcttt ccgcgaagcg ttgcgctgcg gtgcggaaat
     9121 tttccacgcc ttgaaaaaac tgtgcgacag caaaggcttc ccgaccacag tcggcgacga
     9181 aggcggtttc gcccccaacc tgaacagcca caaagaagcc ctgcaactga tggttgaagc
     9241 caccgaagcc gccggctaca aagcgggcga agacgtatta ttcgcattgg actgcgcgtc
     9301 cagcgagttc tacaaagacg gcaaatacca cttggaagcc gaaggccgct cctacaccaa
     9361 cgcggaattt gccgaatatc tggaaggcct ggtcaacgag ttccccatca tctccatcga
     9421 agacgggatg gatgaaaacg actgggaagg ctggaaactg ctgaccgaaa aactgggcgg
     9481 caaagtccaa ctcgttggcg acgacctctt cgttaccaac ccgaaaatcc ttgccgaagg
     9541 cattgaaaaa ggcgtggcaa acgcactatt ggtcaaagtc aaccaaatcg gtactttgag
     9601 tgaaaccctg aaagccgtcg acttagccaa acgcaaccgc tacgccagcg taatgagcca
     9661 ccgctccggc gaaaccgaag acagcaccat tgccgacttg gcagtcgcca ccaactgtat
     9721 gcagatcaaa accggttctt tgagccgttc cgaccgcatg gcgaaataca accaactgct
     9781 gcgtatcgag gaagaattgg cggaagccgc cgactacccc agcaaagccg cattctacca
     9841 actgggcaaa taaaaaaggt taaggtatga agtgggtaac tgtcgtttta tccttcgcac
     9901 ttgtctgttg ccaatacagc ctctggttcg gcaaaggcag catcggacgc aacagcagtc
     9961 tgagagaaca gattgccgtt caagaagaaa aaaaccagac actcgcccta cgcaatcatt
    10021 cccttgccgc cgaagtctat gatttggaaa acggtcaaga agccatttcg gaaatcgccc
    10081 gggtagaact gggttatatc caagacggtg aaacctttta ccgactcatc aggcataacc
    10141 ggtaataccg tcaaaaagcc gtccgaacca atgttcggac ggcttttatt tcaacaaact
    10201 gtcagacagc ccctcatcct cccccgacaa accgcaatcc agcctgacat ccccctcgac
    10261 gcaacagcag cacggcagta tctcgtcccg ccccaaaaaa gccaaaggcg gctcccgata
    10321 agtaacgctt ccctccaaaa tcttcactcg gcacgatccg caatatccgc ttcggcactg
    10381 atattccacc atatgccccg tccgttccaa gccttccaac agggtctcgc cctccaagag
    10441 ctcaaaaaaa cccttattcg taccaatgcg cgccatttcc gaccaatcaa a
//
LOCUS       DAXHMX010000069        10427 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHMX010000069 DAXHMX010000000
VERSION     DAXHMX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.069"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            83..322
                     /locus_tag="ACFM4S_001243"
     CDS             83..322
                     /locus_tag="ACFM4S_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050920.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEELCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
     gene            535..1836
                     /gene="bioA"
                     /locus_tag="ACFM4S_001244"
     CDS             535..1836
                     /gene="bioA"
                     /locus_tag="ACFM4S_001244"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6050921.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVRRAEGVHIE
                     LADGTRLVDGMSSWWCAIHGYNHPVLNQSVEAQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDEADLQPVRVLFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASIKLL
                     LSQDWQANIRRIESILKDRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     KGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1833..2480
                     /gene="bioD"
                     /locus_tag="ACFM4S_001245"
     CDS             1833..2480
                     /gene="bioD"
                     /locus_tag="ACFM4S_001245"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6050922.1"
                     /translation="MKGVYFVSGIDTDIGKTVTTGVLAKQLLQQGKSVITQKPVQTGC
                     QDIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQAYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2495..2971
                     /locus_tag="ACFM4S_001246"
     CDS             2495..2971
                     /locus_tag="ACFM4S_001246"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6050923.1"
                     /translation="MEHLFGKWLPDLPAAISDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSVFEFAVAGEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            3001..3891
                     /gene="ubiA"
                     /locus_tag="ACFM4S_001247"
     CDS             3001..3891
                     /gene="ubiA"
                     /locus_tag="ACFM4S_001247"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6050924.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            4076..4525
                     /gene="ptsN"
                     /locus_tag="ACFM4S_001248"
     CDS             4076..4525
                     /gene="ptsN"
                     /locus_tag="ACFM4S_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6050925.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            4529..5491
                     /gene="hprK"
                     /locus_tag="ACFM4S_001249"
     CDS             4529..5491
                     /gene="hprK"
                     /locus_tag="ACFM4S_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6050926.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNENRPD"
     gene            5472..6326
                     /gene="rapZ"
                     /locus_tag="ACFM4S_001250"
     CDS             5472..6326
                     /gene="rapZ"
                     /locus_tag="ACFM4S_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6050927.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            6407..7348
                     /gene="ylqF"
                     /locus_tag="ACFM4S_001251"
     CDS             6407..7348
                     /gene="ylqF"
                     /locus_tag="ACFM4S_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6050928.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADSERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPADE"
     gene            complement(7439..7559)
                     /locus_tag="ACFM4S_001252"
                     /pseudo
     CDS             complement(7439..7559)
                     /locus_tag="ACFM4S_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7623..8225
                     /locus_tag="ACFM4S_001253"
     CDS             7623..8225
                     /locus_tag="ACFM4S_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6050929.1"
                     /translation="MTEKLPPDALIEAALLTQAEPLNEKTMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            8409..10082
                     /gene="recN"
                     /locus_tag="ACFM4S_001254"
     CDS             8409..10082
                     /gene="recN"
                     /locus_tag="ACFM4S_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6050930.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            10192..10268
                     /locus_tag="ACFM4S_001255"
     tRNA            10192..10268
                     /locus_tag="ACFM4S_001255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10226..10228,aa:Arg,seq:acg)
     gene            10286..10360
                     /locus_tag="ACFM4S_001256"
     tRNA            10286..10360
                     /locus_tag="ACFM4S_001256"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10319..10321,aa:Glu,seq:ttc)
BASE COUNT         2531 a         2618 c         2833 g         2445 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aaaatagggc tgtaaccttc aatcggaatt
       61 tgttgcctgc gggatatgcg gtatgaatgt ttgggatata tggggcagga tggtggaaat
      121 ctatcataag tataagaagc cgtgcctggt tttggcggtg gattttgtga tgggtatggt
      181 attcatagag ccgaatgagg agctgtgcat cggcagatgc tatgcgccta tgtcggagtc
      241 ccctgatttt gctaacgctg ttgcgatggc tgttgctatg atctgtatcg tatggattgc
      301 cgttttgtct atgactgatt gaagcgttgg gcggaggctg tgtgaaacaa tattgggcgt
      361 tgggtcgtct gattccaatc gggcttgggg aatgcgaaac ggtgtgcgct tatactgcgg
      421 acgatttgtt gcgtggtttt acgcccgaaa cggatggaaa ggtgtgggaa gcggtctgtc
      481 ggagtagaat acgcgtttgc gtttgaatac agtaagaaga aaagagagaa acttatgccg
      541 tctgaacatc aacacacatc atcattgctt gatttcgacc gcacccacct gctccatccc
      601 tatacatcta tgaccgaccc gttgcctgtt tatccggtgc gccgcgcgga gggcgtgcat
      661 atcgaattgg cggacggtac gcggctggtt gacgggatgt cgtcttggtg gtgtgcgata
      721 cacggctaca atcatcctgt tttgaatcag tcggttgaag cgcagatgaa acaaatggcg
      781 cacgtgatgt tcggcggttt gacgcacgag ccggcggtgg agctgggcaa attgttggtc
      841 gggattttgc cacaggggct ggatcgtatt ttttatgcgg attcgggttc ggtttcggtg
      901 gaagtcgcgc tgaagatggc ggtgcaatat cagcaggcgc ggggtttgcg tgcaaaacaa
      961 aatatggcga cagtgcggcg cgggtatcac ggcgatactt ggaacgcgat gtccgtctgc
     1021 gacccggaaa cggggatgca ccatattttc ggcagcgcgt tgccgcagcg ttattttgtc
     1081 gataatccga aaagccgttt cgacgatgaa tgggacgagg cggatttgca gcctgtccgc
     1141 gtcttgtttg aagcgcatca tacggacatt gccgccttta ttttggaacc ggtcgtgcag
     1201 ggcgcgggcg gtatgtattt ttatcatccg cagtatcttc gcggattgcg cgatttgtgc
     1261 gacgagtttg atatcgtttt gatttttgat gaaatcgcca ccggattcgg gcgcacgggc
     1321 aagatgtttg cctgcgaaca cgcggaggtc gtgccggata ttatgtgcat cggcaagggt
     1381 ttgagcggcg gctatatgac gctggcggca gcaatcactt cgcaaaaagt taccgaaacg
     1441 atttcgcgcg gcgaagcggg cgtgtttatg cacggcccga catttatggc aaacccgctg
     1501 gcgtgtgccg ttgcctgcgc ttcgatcaaa ctgcttttgt ctcaagattg gcaggcaaat
     1561 atccgccgca ttgaaagcat cttaaaagac cgtctgaaag ccgcatggga cattcgcggc
     1621 gtgaaagacg tacgcgtttt gggtgccatc ggcgtgatcg agctggataa aggcgtggat
     1681 atggcgcgtt ttcaagcgga ctgcgtggcg aagggcattt gggtgcgccc gttcggcagg
     1741 ttggtgtatc tgatgccgcc ttacatcatt tcagacggcg ttttgaccaa acttgccgac
     1801 aaaaccgtgc aaatcttgaa ggaacacagc aaatgaaagg cgtttacttc gtcagcggca
     1861 tagacacgga catcggcaaa accgtcacca ccggcgtatt ggcaaaacaa ttgttgcagc
     1921 agggcaaaag cgtgattacg caaaagcccg tgcaaaccgg ttgccaagac atcgccgacg
     1981 acatcgccgt ccatcgtaaa atcatgggta aacccatgca ggaagccgac aaacaagggc
     2041 tgaccatgcc tgaaatcttc agttatccag cttcgcctca tctcgctgcc cgtcttgacg
     2101 gcagggcttt ggacttggac aaaatccgca ccgccacaca agaattggcg gcgcagtacg
     2161 aagtcgtttt ggtcgaaggc gcgggcggat tgatggttcc gctgacggaa aacctgttaa
     2221 ccattgatta tattcggcaa caggcttatc ccgtcatcct cgttaccagc gggcggctcg
     2281 gcagcatcaa ccacaccctg ctcagtttcg ccgcgctcaa acaatacggc atccgtctgc
     2341 acagcctcgt gttcaaccac atccacgaca gccgcgacgc acacatcgcc caagacagcc
     2401 tgagctactt gaaatgccgt ctgaaagccg atttttccga agcggaatgg atggagttgg
     2461 caaaaacaga cgcggtataa agattgggaa aaatatggaa cacctatttg ggaaatggct
     2521 gcccgacttg cccgccgcca tttcagacgg catcagcctg ccgatggtgc ggctgctgca
     2581 cacccggtcg ctgaccgccg cattgcgcgc cttgccgcat acattttcgg tggaactgct
     2641 gaaactgggc gaattggaga cggaatgcgg agggaggctg gtgcgcgaag ttttgttgaa
     2701 gctggaccgt atccctgttg ttgaggcaag gagcgaatgc cgtatcggtt cggcgttttg
     2761 gcaaaacatt ttggactgcg gcacgcgtcc tttgggcgag cgtctgtttc aagccgattt
     2821 ggaaggggcg cgttcggtgt ttgagtttgc cgttgccggc gaaggatgcg gacggtactt
     2881 tgccgcgcgg cgttctcggt tttcccatca aggcgaggaa atgctgctga ccgagtattt
     2941 tctgcccgaa ctgaaacgct ttatcggata aaataccgtt ttttcaagct gtgcggcaat
     3001 atgaatccta aatccccttt atttttacga ctgtccgacc gtttggatgt gtacctgcgc
     3061 ctgatgcggg cggacaagcc cattgggacg ctgcttttgc tgtggccgac ctactgggcg
     3121 ttgtggctgg cttcagacgg tattcccgat ttggcggtat tggcggcgtt tacaatcggc
     3181 acgtttttaa tgcgcagtgc cggctgcgtc atcaacgatt ttgccgaccg cgattttgac
     3241 ggggctgtcg agcgcactaa aaaccgtccg ttcgcacagg gcagggtcaa gaaaaaagaa
     3301 gcgctgctgc tgacggcatt tttgtgcctg cttgccgcat tgtgcctgat tccgctgaat
     3361 catctgacat ggctgatgag tctgcccgcg ctgtttcttg cgctgactta cccgtttacc
     3421 aaacgctttt ttccgattcc ccaactctat ctcgggcttg ccttttcctt cggtatcccg
     3481 atggcgtttg ccgccgttgc cggaaacgtg ccgcctcaag cgtggatact ctttgccgcc
     3541 aatgtgttat ggactctggc gtatgacacg gtttatgcaa tggcggacaa agaagacgat
     3601 ttgaaaatcg gcatcaaaac ctccgccgtc acgttcgggc gttacgacat cgccgctgtt
     3661 atgctgtgtc atggcggctt taccctgctg atggcagtat tgggggcggt tatcggtgcg
     3721 gcatgggcat attggacggc aatccccatc gtcctgctgc tgcaataccg ccaatatgcc
     3781 gccatcaaaa gccgcgtccg gcaaatctgt tttgaaacgt ttttggcaaa caacagaatt
     3841 ggttgggtgt ggtttaccgc catttttgcc catacgtttt tcgcgaaata aggcagggca
     3901 atgccgtctg aagagccgta aactgctttg gacggcattt ctatctgtgc cgaaaagcgt
     3961 taaaatatgt ttttaaaacg ctgtgttatg tcagcccgta ccgtatgcgg gattgagatt
     4021 tgccccggca gtcggtacaa tctttctgtt ttgcgatgtc tgaaaagaga agcttatgag
     4081 ccttatcggc gaaattttgc ctttgtccca tattgttttg gatatggagg tgggcagtaa
     4141 aaaaaggctg tttgaggaag caggcctgct tttggaacgc gaatcctcat tgtcccatgc
     4201 tgatgttttc gaatgtcttt ttgcccgtga aaaactcggt tcgaccggtt tggggcaggg
     4261 cgttgccatc ccgcacggcc gtcatgccgg cgtgaagcag gcgacgggcg cgttcatccg
     4321 cacgcgcgaa cccgtcggat ttgacgcacc ggacggcaaa ccggtttccc tggtttttat
     4381 cttgctggtt cctgagaatg caacgggcga gcatttggag gtattgtcca aactggccgg
     4441 caagttttcc caaaaaagca tccgcgaatc gctgatgacg gtttcttctg cggaagaagc
     4501 ccgcgccatc ctgaccgaag aataaaatat gcccagtatc tctgtccgcc gactgtttga
     4561 cgacaaccaa tacaagttgc aacttgcctg ggccgccggc aattcgggtg cggacaaccg
     4621 tatcggcgta gaggcggaca agcccgtcct cgccttggtc ggacacctga atttcatcca
     4681 ccccaaccaa atccaagtgg tcggtttggc ggagtcggaa tatctgaacc gtctcgaatc
     4741 gggggaaacg ggttatcagt tcggagacct gttcgacatt tctatgtctt tggtcattgt
     4801 ggcaaacggc ttgccggttt ccccgggtct gcgcgactat tgccataaaa acgatattcc
     4861 actgctgact tccaaactcg aaagccccta tctgatggat gtgttgcgga tttacctgca
     4921 acgcaccctg gcggcatcgt ccgtcaaaca cggcgtattt ctcgatgtgt ttgaaatcgg
     4981 cgtgctgatt accggacatt cgggtttggg taagagcgaa ttggcattgg aactgatttc
     5041 gcgcggccac agcctgattg ccgacgatgc ggtcgagctg ttccgcatcg gcccggaaac
     5101 attggaagga cgttgttcgc ctatgctgcg cgattttttg gaagtacgcg gcttggggat
     5161 actcaatatc cgccatattt tcggcgaaac ttccatccgc cccaaaaaaa tcctgcaact
     5221 cattatcaat ttggtcgagg cggacgacga gtatatgaag cagcttgacc ggttgagcat
     5281 ccgcaccgaa accgaatcca tcctcaacgt caacgtccgt tcggttacgc tgcccgtcgc
     5341 cgtcggacgc aacctcgccg ttttggttga ggcggcggta cgcaattata ttcttcagtt
     5401 gcgcggtaag gatagtacgc gcgaattttt ggaacgtcat cagacgcagc ttaaagaaaa
     5461 cgagcaacac aatgaaaatc gtcctgatta gcgggttgtc cggttcgggc aaatccgtcg
     5521 cactgcgcca aatggaagat tcgggttatt tctgcgtgga caatttgcct ttggaaatgt
     5581 tgcccgcgct ggtgtcgtat catatcgagc gtgcggacga aaccgaattg gcggtcagcg
     5641 tcgatgtgcg ttccggcata gacatcggac aggcgcggga gcagattgcc tctctgcgcg
     5701 gactggggca cagggttgaa gttttgtttg tcgaggcgga agaaagcgtg ttggtccgcc
     5761 ggttttccga aaccaggcga ggccaccctc tgagtaatca ggatatgacc ttgttggaaa
     5821 gcttaaagaa agaacgggaa tggctgttcc cgcttaaaga aatcgcctat tgtatcgaca
     5881 cttccaagat gaatgcccaa cagctccgcc atgcagtccg gcagtggctg aaggtcgaac
     5941 gtaccgggct gctggtgatt ttggagtcct tcgggttcaa atacggtgtg ccgaacaacg
     6001 cggattttat gttcgatatg cgcagtctgc ccaacccgta ttacgatccc gagttgaggc
     6061 cttacaccgg tatggacaag cccgtttggg attatttgga cggacagccg cttgtgcagg
     6121 aaatggttga cgacatcgaa aggtttgtta cgcattggtt accgcgtttg gaggatgaaa
     6181 gcaggagcta cgttaccgtc gccatcggtt gcacgggagg acagcaccgt tcggtctata
     6241 ttgtcgaaaa actcgcccga aggttgaaag ggcgttatga attgctgata cggcacagac
     6301 aggcgcaaaa cctgtcagac cgctaattcc gtcaaaccat tatgccgtct gaaaccccgt
     6361 cctccgtttc agacggcatt tcaaatattg aaaagaaaga acagacatgg caatccaatg
     6421 gtttcccggc catatgaaca aggcgaaaaa agccatcgcc gagcgtgcaa aaagcgttga
     6481 tatggtgatt gaaatgctgg acgcgcgtat gcccgcctcc agcgaaaacc ccctgcttgc
     6541 ccagctttcc aaaggcaaac ccaaacttaa aatcctaaac aaacaagatc ttgccgactc
     6601 cgaacgcaca aaggtctggc ttgaccacta caacagccgc cccgacacct gcgccattgc
     6661 cctcgattcc tccgaaaccg gcgcacacgg caaaatcacc caagcctgcc gcgcgatgat
     6721 tccccaccgg caaggcatag acaaaccctt gcgcgtcttg atttgcggca ttcccaacgt
     6781 cggcaaatcc accctcatca acggcatgat aggcaaaaaa tccgccaaaa ccggcaacga
     6841 acccggcatc accaaagccg aacaacgcct cttcctcgcc gatgacttct ggctctacga
     6901 cacccccgga atgctgtggc cgaaaatcat cgtcgaagaa agcggctaca accttgccgc
     6961 cggcggcgca gtcggacgca acgcgttgga cgaagaagaa gtcgccctcg aacttttaga
     7021 ctacctccgc cgccactacc tccctatgtt gcaagaacgc taccaagccg acaaagaccc
     7081 cagcagccac tgggacgaaa acgtttggct cgaatggata gccaaaaaac gcggcgcagt
     7141 cctcagcggc ggacggatca actaccaaaa agccgccgaa aacatcctta ccgacttccg
     7201 tgaaggcaaa atcggcagaa tcaccctcga aacgccgaac caatgggaaa cttggctcaa
     7261 aaaagcccgt cagaaagaag ccgaactcaa agccatacgc gaagccagaa aagcagagcg
     7321 taaagggcag aagcctgcgg acgagtaggg cggtatagtg gaatgccaaa agaggtttca
     7381 ctatcttcaa acaggtcgtc tgaaacctca gaataaattt ttcagacgac ctgtttgtaa
     7441 aatttaaaac caacatatat agtggattaa ctttaaacca gtacggcgtt gccttgccgt
     7501 actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaagttaa tccactataa
     7561 aactgaaaga acccttatcc aaggctgtct gaaaaatatt taaactatta aatccggatg
     7621 ttatgactga aaaactccct cctgatgcgc tgattgaagc cgcgcttttg actcaagccg
     7681 aacctttaaa cgaaaagacc atgcgcgaat tgtgtgtgcc gccgttgtcg caagacaaac
     7741 tgattgatgt gttggcgcag ttgaaaacgc gttggcagga tagggcgttg caactggtgc
     7801 atacgcaaga gggctggcgt tttcagattg ttcagacggc attcgagcgg ctgggcagcc
     7861 tgcaagaaca gcgtgcgccg cgctactccc gcgccgtgat ggaaacactg gcgattatcg
     7921 cctaccagca gcccgtaacg cgcggcgaca tcgagggcat acgcggcgtg gcggtgtcgc
     7981 agaacgtgat gcagaccttg caggatcggg ggtggattga agtcatcgga catcgggaca
     8041 cattgggaaa acccgcattg tgggcaacaa cggcaacgtt tctcagcgat ttgcgcttgg
     8101 acggtctgga agaacttccg ccgctgaccg aactgggcga actggtttta cccgatttaa
     8161 tggaaatgcc gcctacggat gaagaagagc cggaagccgt accgtccgat accctgccca
     8221 actgaaattc caaatgccgt ctgaaacgca cattgcttca gacggcattg caacaaataa
     8281 gcagataaaa acaagtacta agcaaaatta aggaaaactt atttttaatt taaaaaaatc
     8341 ttagttataa ttcgtatatc taaagttgat attgcttttg tcggtagaat tgctaaggaa
     8401 tcctcacaat gcttctaaca ctttctttgc gtgattttgt cattgttgaa aatctgaatc
     8461 tggattttca aagcggcttt accgtattga ccggagaaac cggcgcgggc aagtccatta
     8521 ctttggatgc gattggtctg ctgttgggcg acaaagccga ttacagccaa gtccgcagcg
     8581 gtgcaaaaga agcgcagttg tcggcattgt tcgatatttc ccatttgcct gttttaaaag
     8641 cagaattgta tgaacagggg cttttaaacg acggagaaga agaactcagt atccgccgca
     8701 ttatcgatgc caaaggcaaa agccgcagct ttatcaacaa tcaggccgct accttggcgc
     8761 aactcaaatc cgtcggtagc cagcttatcg acatccacgg gcaaaacgcc catcattcgc
     8821 ttaatcagga agccgcccag cgcgaattgt tggacgcatt tgcgggtagc agggagcagg
     8881 cggaaaccgt caggcagctt tatcaaaatt gggccaatgc gaaaaaagcc ctccaagagg
     8941 cgcaggaaca cgccgatgcc gtcattatcg agcgggagcg tctggaatgg cagtttaacg
     9001 aactgaatca gttggacatt aaacaaggcg aatgggaagc cctcagccaa agccacgaca
     9061 gccttgccca ttctgccgag ctgttgcagg ctgccgaaga agtcggaagc aagattgacg
     9121 gcgacaacgg catccaacgc catatctatc aatgtcaaaa actattggcc aatctgcaaa
     9181 acatcgagcc gcgctttgcc gagagcctga atatgttggc aagcatcgaa gccgaattgg
     9241 gcgaaatcag tgccaatatg cgcgatgtgg caggtcgcag cgacatcaat cccaatgaat
     9301 tggcggcaca ggaacagcgc atgggcgaac tgatggggat ggcgcggaaa taccggatcg
     9361 agcctgaaga gttgcctgcc aagttggcag aaatcgaaga acgcctgcaa agcctgcaag
     9421 ctgccgccga tttggacgcg ctcgagcata atgttgccca caattttgcc gaatatcagg
     9481 aagctgccca catcctttct gccatgcgcc atcaggcggc agagcgtttg agcggcgaaa
     9541 cgaccgagca tatgcaacac cttgccatga aaggtgcacg tttcgacatc gtcctgttgc
     9601 cctcgtcgcc gacggcacat ggtttggagc aggttcaatt tcaagttgcc gccaacaaag
     9661 gcaatccgcc ccgtccgctg aataaagttg cctccggcgg cgaattggcg cgtatcagcc
     9721 ttgccttaca ggttgttgcc agccaatata cccaagttcc caccctgatt tttgatgagg
     9781 tcgataccgg tattggaggg ggagtggctg aaatggtcgg caaggcatta cgttcgttgg
     9841 gcagaaaaca tcaggtgctt gccgttaccc atcttccgca agtcgcatcc tgcggcgaaa
     9901 accactggcg ggtgcgcaag cacagcgagg gagagcaaac cgtcagcgaa atcagtatat
     9961 tggataaaat ccaacgggtc gaagaggttg cccgtatgtt gggcggagaa gtcattaccg
    10021 atacgacgcg gcaacatgcg gcagaattgc tgcaacttgc gtcgaaaaat agtttatttt
    10081 aaaatcaatc agttaaaaaa taactaaaaa taaaagtcta aaacaataga cagaactcag
    10141 ataaatccgt attatcacgc tttcttaatc acttgaacaa gtgattgtgc tgcacccgta
    10201 gctcagttgg atagagtatc tggctacgaa ccagagggtc gggcgttcga atcgctccgg
    10261 gtgcgccagt aagaaaatac aatatgcgcc catcgtctag cggttaggac atcgcccttt
    10321 cacggcggta accggggttc gattccccgt gggcgtgcca attcaaaatg cctccgatta
    10381 tatcggaggc atttctcatt tctcatttct catttctcat ttctcat
//
LOCUS       DAXHMX010000070        10318 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHMX010000070 DAXHMX010000000
VERSION     DAXHMX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.070"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..141
                     /locus_tag="ACFM4S_001257"
     CDS             <1..141
                     /locus_tag="ACFM4S_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFC6050931.1"
                     /translation="VGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRHI
                     RT"
     gene            270..1706
                     /locus_tag="ACFM4S_001258"
     CDS             270..1706
                     /locus_tag="ACFM4S_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6050932.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1717..2700
                     /gene="patB"
                     /locus_tag="ACFM4S_001259"
     CDS             1717..2700
                     /gene="patB"
                     /locus_tag="ACFM4S_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6050933.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTFSDGIKAFLSGETPSTAQGGGSADMPSEAAASEAVPQTGKT
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2697..3890
                     /locus_tag="ACFM4S_001260"
     CDS             2697..3890
                     /locus_tag="ACFM4S_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6050934.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKIRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(3950..4126)
                     /locus_tag="ACFM4S_001261"
     CDS             complement(3950..4126)
                     /locus_tag="ACFM4S_001261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050935.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAVCRRFACVLPMPEANAGCI
                     WQTVRGVAMPSEAI"
     gene            4177..5730
                     /locus_tag="ACFM4S_001262"
     CDS             4177..5730
                     /locus_tag="ACFM4S_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6050936.1"
                     /translation="MNRTYANFYEMLTTACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASVGLSKELAGLKAQTPIEKIIWTGKSRPVGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5821..7848)
                     /locus_tag="ACFM4S_001263"
     CDS             complement(5821..7848)
                     /locus_tag="ACFM4S_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6050937.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFGWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8137..10143
                     /locus_tag="ACFM4S_001264"
     CDS             8137..10143
                     /locus_tag="ACFM4S_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6050938.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGADSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLTIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         2474 a         3219 c         2494 g         2131 t
ORIGIN      
        1 gtcggtatcg gcagcggcgc actgttggga cattgggtaa cgcaatatgt cggtatttcc
       61 cgtatcggca tcgccggtat gcttgcggcg gcggccgggc tgtggctgtg cctgaacttg
      121 aaccgccata tccgaacctg acccgctatg ccgtctaaag cctctcccgc ccttcagacg
      181 gcattttgat tgaagaaata tccgcccctg cttaaaataa cgggctttgc cgtgttttaa
      241 cgcctatttt cttattccaa ggatggttta tgccgctgct gtctgtcgag ttcgcactgt
      301 tctttctcgc cttcctgccg atttactggg gcttggcgaa atacccgtcc gtccaaaacc
      361 tgctgctttt ggctgccggt atgggctggc tctaccatat cggccccgta tttgcggcaa
      421 tcgtcgtcct ttattcctcc tgcgtgtacc ttttgggcga actgctccgt tccgatcgcg
      481 aaaatacgcg ccgcttttgg ctggggtgcg gcattgccgt ctcgctgacc gtcttgggct
      541 ttttcaaata tttcgacttt ttccgcccgc tgattgcaca atatgccgga caaggcggcg
      601 caatcgacat cctgatgccg ctggggcttt cgtattacac cttccagtcg ctcgcctatc
      661 tggtttactg cttccgcgcc ccgcacgccg cgcgtttcga gtggcacgag ctgctgctgc
      721 acctgagttt tttccccacc gttacctccg gcccgattat ccgcgccgcc gcattcaaaa
      781 gcgcagacgg cgagcaggcg ggcgcattgg cgcaaatccg tacccgccaa ccgcgctcac
      841 ccgtccgccc cgcactcgcc gtttccctga ttctgctggg tattgccaaa aaatggtggc
      901 tggcggggat gctggcggaa aactgggtgt cgcccgtatt tgaaaatccc gcccaattcg
      961 acggctgggg cgtattgggc ggcgtgtacg gctatacctt ccaactcttt ttagactttt
     1021 ccggatattc cgatttggtt atcggcatgg cgatgctgct gggtttccgg ctgcccaaaa
     1081 acttctccgc accgcttcgt gctttaaaca tccgcgcatt ttgggacaaa tggcacatca
     1141 gcctttccac ctggatacgc gactacatct acatcccctt gggcggcagc aaaaaaggct
     1201 ttttacggac acagctcaac ctgatggcgg caatggtact ctcaggcatc tggcacggct
     1261 acggctggaa cttcctcatt tggggcgcgc tgcacggcac ggcactggtg ctgctcaaca
     1321 cgggcgatcg ctatttcgga cgcaacgcgc tatgccgtct gaaatacctt gcgccgctct
     1381 cgtggtttgt taccttccat ttcgtctgcc ttagctttgt tgtcttcaat accgcaagcc
     1441 ccgacgatgc aggcgcagtt ttcaatgccc tctttgccaa tgccaacggc tggaatgcgc
     1501 cgcaacaggc aaacatgctg ctgcttgcct cgtttgcatc cgtgatgctg ctctaccctt
     1561 acctgcaacg cgctttcgac ggcgcggtca aaggtttgga aaaaatcccg atgtggctgt
     1621 ggtttatccc cgtttccgcc gtcctgctgc tgattatcgt attggccccc tcggggattc
     1681 ccggctttat ttatgccaat ttttaagggt ttggacatga aaaactttct ttcccttttc
     1741 gcctccatac tgatgtctgc cctgattgcc gtgtggttca gccaaaaccc catcaacgcc
     1801 tactggcagc agacctacca ccgcaacagt ccgctcgaac cgcttgccgc ctacggatgg
     1861 tggcggagcg gtgcggcgtt gcaagaaaac gcctacacct tttcagacgg catcaaagcc
     1921 ttcctgtccg gcgaaacgcc gtcgacggct caaggcggcg gttcggcaga tatgccgtct
     1981 gaagccgccg catcagaagc cgtccctcaa accggtaaaa cagaatggaa acaagacacc
     2041 gaagccgccg ccgtccgcag cggcgacaaa gtcttttttg ccggcgactc gctgatgcag
     2101 ggcgttgcac ccttcgtgca aaaaagcctg aaacagcaat acggcatcga atccgtcaac
     2161 ctcagcaaac aaagcacggg gctgtcctac ccctcattct tcgactggcc gaaaacgatt
     2221 gaagaaaccc tgaaaaaaca tcccgaaatc agcgtgctcg ccgtcttcct cggcccgaac
     2281 gacccgtggg atttccccgt cggcaaacga tacctcaaat tcgcttccga cgaatgggcg
     2341 caagaatacc tgaaacgcgt cgaccgcatc cttgaagccg cacacacgca ccgcgtccaa
     2401 gtcgtctggc tcggcatccc ctatatgaaa aaagccaagc tcgacgggca gatgcgctac
     2461 ctcgacaaac tgctttcgga acacctggaa ggcaaaatca tcctgattcc caccgcgcac
     2521 accctgagcg gcggggaaga ccgctacacc gactccgtca acgtcaacgg caaacccgtc
     2581 cgctaccgca gcaaggacgg catacacttt accgccgaag gacaaaaact gctggcggca
     2641 aaaataatgg aaaaaatcgt ttttgaacca agtacgcaac catcaagtac acagccatga
     2701 accccaaaca cctcatcgca ttttccgccc tgttcgccgc cacgcaggca gaagccctgc
     2761 ccgtcgcctc cgtcagcctc gacaccgtta ccgtttcccc gtccgccccc tacaccgata
     2821 caaacgggct cttgaccgac tacggcaacg ccgccgcctc gccttggatg aaaaaaatcc
     2881 gatccgtcgc acaaggcagc ggcgagacct tccgcatcct gcaaatcggc gactcgcata
     2941 ccgccggcga cttctttacc gacagcctgc gcaaacgcct gcaaaaaact tggggcgacg
     3001 gcggcatagg ctgggtttac cccgccaacg tcaaagggca gcgcatggcg gcagtccggc
     3061 acaacggcaa ctggcaaagc ctcaccagca ggaacaatag cggagacttc ccgctcggcg
     3121 gcatcctcgc ccacaccggc agcggcggca gcatgaccct gaccgcatcg gacggcaaga
     3181 ccggcaaaca gcgcatttcc ctgtttgcca aacccctgct cgccgaacaa accctgaccg
     3241 tcaacggcaa caccgtctcc gccaacggcg gcggctggca ggtactggat acccgcgcgg
     3301 cactgccctt gaccatacag accgaaatgc cgtgggacat cggcttcatc aacatcgaaa
     3361 atcccgccgg cggcattacc gtttccgcca tgggcatcaa cggcgcacaa ttgacccatt
     3421 ggtcgaaatg gcgtgccgac cgtatgaacg accttgccca aaccggcgcc gatttggtta
     3481 tcctttccta cggcaccaac gaagccttcg gcgacaacat cgacattgcc gacaccgaac
     3541 aaaaatggct ggataccgtc cgccaaatcc gcgacagcct gcctgccgcc ggcatcctca
     3601 tcatcggcgc acccgaatcc ctgaaaaaca cgctcggcgt atgcggcaca cgccccgtcc
     3661 gcctgaccga agtccaacag atgcagcggc gcgtcgcccg acaggggcag acgatgttct
     3721 ggtcttggca aaacgccatg ggcggcatat gcagcatgaa aaactggctc aaccaaggat
     3781 gggccgccaa agacggcgta cacttctccg ccaaaggcta ccggcgcgcg gcggaaatgc
     3841 tcgccgacag cctcgaagaa ctcgtccgcg ccgccgcaat caggcaataa tcggacaggg
     3901 ggcggacggt atttccgcaa cagggggatg ccgtctgaaa cgcatacctt catattgctt
     3961 cagacggcat agccaccccg cgcacggttt gccagatgca accggcattc gcctcaggca
     4021 tcggaaggac gcaggcgaac ctccggcata cggcgcaaag gcggcgtttg atatgccgtc
     4081 tgaaggcaaa gatgataaac tgccgccttc cgttttcaga cggcatattg ttttcaaatg
     4141 agggcgttcg ccgtccgcaa ccataaagga agtttcatga accggactta tgccaatttc
     4201 tacgaaatgc tgaccaccgc ctgccgcaaa aacggcaacg gcacggcagt gttcgacggc
     4261 aaggaaaaaa ccgcctaccg cgcgctcaag caggaggccg aagccgtcgc ggcgtatctg
     4321 caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg cggtttccaa ttccacagaa
     4381 tttattaccg cctatttcgc catctccgcc atcggcgcgg tcgccgtacc gatgaacaca
     4441 tttttgaaaa acagcgaata cgcgtatatc ctgaacgact gcaaggcgcg cttcctgttc
     4501 gcctcggtcg gcctgtcaaa agaattggcg ggcttgaagg cgcaaacgcc catcgaaaaa
     4561 atcatttgga cgggcaaaag ccggccggtc ggcgaaacgg cggaaggcga tgcctttttt
     4621 gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc gacaaccccg gataaatgat
     4681 ttggcacaca tcatctacac ctccggcacg acggggcatc ccaaaggcgc gctaatcagt
     4741 tacgccaacc tgttcgccaa cctgaacggc atcgaacgca tctttaaaat ttccaaacgc
     4801 gaccgcttta tcgttttcct gccgatgttc cacagcttca cgctgacggc tatggtgctg
     4861 ctgccgattt atatggcgtg ttcgattatt ttggtcaaat ccgttttccc gttttccaat
     4921 gttttgaaac agaccctgct caaacgcgcg accgtgtttt tgggcatacc cgcgatttac
     4981 accgcgatga gcaaggcgaa aatcccttgg tatttcagat ggttcaaccg aattcgcctg
     5041 tttatcagcg gcggcgcgcc tttggcggaa caaaccatcc tcgatttcaa agccaagttc
     5101 ccccgcgcca aattgctgga aggctacgga ctgagcgaag cctctcccgt cgtcgccgtc
     5161 aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc ccctgcccgg tttggaagcc
     5221 aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg aagtgggcga actgattgtc
     5281 aggggcggtt cggtgatgcg gggctacctc aatatgcctg ccgccaccga tgaaaccatc
     5341 gtcaacggct ggttgaaaac gggcgatttc gttaccatag acgaagacgg cttcatcttt
     5401 atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc aaaatgtcta tccgcgcgag
     5461 atcgaagaag aaatctacaa actcgatgcc gtcgaagccg ccgccgtcat cggcgtgaaa
     5521 gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat tgaaggaagg tatggatttg
     5581 ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg caaatttcaa aatccccaaa
     5641 caaatccact ttaaagacgg gctgccgcgc aacgctacgg gcaaggtatt gaaacgggtg
     5701 ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc gtccgaagcc ccgtccggca
     5761 aaaaaaatgc ggtgaatctg cttcaccgca tttttttggc aaacgccgga gcgaggatat
     5821 ttactctgcc aattccacct gctcgtgcgc catcagttcc tgaccgacat catccaacaa
     5881 catcaaataa cgttcgtagt ttttcaaaat gtcggcaatc agctcgtcct tgttcatata
     5941 ctgcacatcg tacccgacgc gcccgtcgaa aaaataagcg tagggtttgt aagttgtctg
     6001 atgccggata tgcggcagct tgccgtcgtt aatcaactgg tcggatacat cctgcccgac
     6061 agacttaatc ccgtacataa aatcgcgcat cgtctctttc cgaatgacga actcgattgc
     6121 gggctcgtcc tgatgaaaca tcttatcgac ccggacgctc aagccgtatt cttccgaaag
     6181 ctcccgttgc aactcgtgca tagcgggcga tgcggtatgt ttgaggaatt ttaaaatatc
     6241 ctgctcctgc gtctggctca ttatctgcac cagccgttct ttccacttgc cgcccgtcca
     6301 aaatacactg gtagggttaa cccgggtctc aaaatatttc ttatccgcac tcaagccttt
     6361 ccacaggctg aaacacatta tcagcatcag cagggcaaac ggcagggaaa caatcagggt
     6421 catagactgc aggttgccga gtccgcccga gcgcatcagc aaaacggcaa cggcagacat
     6481 cagcacgccc cacataaccg cctgccaccg tggcgcgctc aagcctttgt cccgagaggt
     6541 aatattgttc aggacataaa tcccggaatc ggcagaagtt acaaaaaaca gagaaatgac
     6601 cagcaggctg acgatgctcg tcaattcggg cagggggagg taattaaaga atttaaaaag
     6661 cagcgtttcc ggagaggagg tcatcttttc gagcattccc cccgcaaccc cgtcattcag
     6721 ccaaatcgcc gtattgccga agacggtaaa ccacaaaacg ccgaacaggc cggggatgag
     6781 caaaaccccg aagacaaact cgcggatggt gcgccccttt gaaatgcgcg cgataaacaa
     6841 acccacaaac ggcgcccaag aacaccacca cgcccaataa agcaccgtcc aagattcaaa
     6901 ccacggcttg tgttcccgtt cgtacgcata agttttaaaa ctgaggcgca ccagatttcc
     6961 gaggtagttc cctatgttgt cgccgaatgc cgacaacagg taaacagtgg gacccgccgc
     7021 caaaacaaaa aacagcagca aaaacgcaag gctcaggttc aactcgctca acaccttcac
     7081 gcccttcccc acgccggata ttgccgaaac gacggcgagg gacatgacgg cggcgataat
     7141 caaaacctgc acgctgaagc tgttttcggc aatccagccc atttcctgca atccggcgcc
     7201 cagttgcgaa gccccgaacc ccaatgtggt gatgatgccg aaaaaagtag caagcaacgc
     7261 cataatatca atggcatcgc cgaaccttcc ggaaattttt tctttcaaca ggggataaaa
     7321 acaagaacgc agggcaagcg gcagcttgta gcggaaaccg aaataagcca aagccaatgc
     7381 aatcgtaccg tacaccgacc aagcgtgaac gccccaatgg aacaccgtgt gcagcaatgc
     7441 ctgctgctgc ctgtgttccg gcgtgccggc cgtaatgtcc gaaaaataat gcatcaacgg
     7501 ctctgccacg ccgaaaaaca tcagacccac gcccatcccg gccgcaaaca gcatcgccag
     7561 ccacgacagg aagccgaatt ccggcacatc ttcatcccgt ccgagcctga tgtttcccaa
     7621 actgctgacc gagagtatca gcaggaaacc cagaaaaatg gaaaacgtta aaacataaaa
     7681 ccagccgaac tcggtaaaaa tgacttcttt tgcccgatcg agccacatct gcacctgatc
     7741 cggcacggtt aaaaccaata ccaccaaaac acacacaaaa aacaaagtcg tcaaaataac
     7801 catcggatta aatgacgttc ggcgttctat aaattcagac agggacaaac cttctcactc
     7861 ctttgttaaa aacagacaaa cccggtcatc gggcaaagcg gtcaaaacct gccgacaaaa
     7921 taccggcttc cggatgacga aatgcacaaa ccggccgaaa ttgttataat cgaaaaaatt
     7981 aaaaatcaat acggattatt ctatcagatt gatttatcga tatttattat atcattaata
     8041 tagtttgatt tcaaaccggc ggaaaatgcg ccgccttccg caggcgcgcc ctccccgcca
     8101 tcggggcgtt taacgccgga ttgccgatgc ggtacaatgg ctgatatgaa gaaaattacc
     8161 cctcaaaacc tgcgccccct gctttcggaa agcttgggac ataccgattt tgtcaacgtc
     8221 ctcaacgcac tgattaaatt tttgcgccgt ggcggcaaaa aatgtgcggg ggagcgtttc
     8281 gacctgatta tcgacacatt caaacaagac agggaattgc tgtcccgctt cagccggtgt
     8341 ttttacatct ggctcgcgca aatacacatt tatccggcac tcatcaaact cggcatcttc
     8401 tcgcgccaca gctttgcccg agaaatgggc atacgcatct acgaacgctt cagcccgtca
     8461 tataaagatt ttgccaactt gggcgaagtc ttcctttatc ttttccattc cgaaaacgac
     8521 gacaaatggc tgcaaacgct caatatccgc caatggctgg ttttatacga actcatccgg
     8581 agccacgccg agccgtccaa attgcagacg gcgggcatcc gccttgccga tgcgcgtttg
     8641 cgcgccatcg aaatgctgtc tgtctggacg gcatccgaag ccatcgaacc cgacctcatc
     8701 cgcatcgccc cgcgcctgct ggaagccgat tcttccttcg tcgccctcca acgcgaaacc
     8761 gccaaactgg tcgaacacta ccgcaacggc accacgcctt acgacaccgc ccacctcgaa
     8821 gtgatgttcg accaatgttt cagccagatt gactatttgc gccgcaaagg gacgggcgcc
     8881 gactccggtt cgtcggtcaa agtcgcccac ctgctcgaac ggctccggca gaccgtagac
     8941 cgtctaaagc tgctcaccga catccaaacc ggtgccggca acagcaaccg cctgaccatc
     9001 gcgctgatga actccctcat ctacgcggcg gtcgaacaat acagcacccg ccacctgcgc
     9061 cgcagcagca tccgtatgct cgcccgcagc attaccgaaa acaaaagcca ccacggcgaa
     9121 cactacatca cccgcaaccg caaagaatat ttcaaaatgt tctactcggc ggcaggcggc
     9181 ggcatcatca tcgccctgat ggcgctgctc aaaatccgca tcggcacact cggcctcagc
     9241 cccttcctga cttccttgtc ggctgggttc aactacggca tcggctttat gatcatccat
     9301 atgctgcact gcaccgtcgc caccaagcag cccgcgatga ctgccgccag ctttgccgaa
     9361 caggtcgatc tcaacgaagg cggcaaagcg gtggacaaca aactcgccaa gctcctcatc
     9421 gacgtatgcc gctcccaaag tgtcgccgtc ttcggcaacg tttccatcgc catccttttg
     9481 gcgtgcgcca tatcgttcgg ctatgcccat ctgtaccggc tgcccatact cgatgcccac
     9541 accgccgcct accagttcaa atccatagac atcatcgctt acccgacgct gtggtatgcc
     9601 gccattgcag gtctgtggct gttctgctcc ggcatcatcg caggtttttt cgacaaccgc
     9661 gccgactacc tcaacctgcg ccaacgcctg cccttcaacc ccttgctgcg taaaatcatg
     9721 cgccccgggc cccgccgcgt cctcgccgcc tacatccaca aacactacgg ctcgttggta
     9781 ggcaacttca tcttcgggat gctcttgggt atgaccggct atttcggaca cctcctcggg
     9841 ctgccgctgg acatccgcca cgtcgccttt tcctccgcca acctcggcta tgccgccgtc
     9901 agcggcaacg tcggtttggg cacgttcgta ctcggcattt tcagcgtcct caccatcggc
     9961 ctggtcaacc tctgcgtcag tttcagcctc gccctcttcg tcgccctgcg ctcgcgcggc
    10021 acgaaaatcg gcagcatccg caatctgatt aagagttttt ggaatcagat taaaagcaat
    10081 ccctgcatac ttttcctccc gcccgccaaa gaacagggac atcctccttc ggacaagcct
    10141 tgaccggcaa tgccgtctga agcgggcttc tccccgaata ccgccctgat gcggaaaatc
    10201 cccataaaag gatgcaaaaa tgccgtctga accgaaacgt ggttcagacg gcattttaaa
    10261 aggggctgta ctagattagc agatatgtta ccctcgaaat atgaagataa cgcactgc
//
LOCUS       DAXHMX010000071        10214 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHMX010000071 DAXHMX010000000
VERSION     DAXHMX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.071"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(681..1760)
                     /gene="apbC"
                     /locus_tag="ACFM4S_001265"
     CDS             complement(681..1760)
                     /gene="apbC"
                     /locus_tag="ACFM4S_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6050939.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAVADSVQEILMPETGGAHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1971..2046
                     /locus_tag="ACFM4S_001266"
     tRNA            1971..2046
                     /locus_tag="ACFM4S_001266"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Lys,seq:ttt)
     gene            2125..2634
                     /locus_tag="ACFM4S_001267"
     CDS             2125..2634
                     /locus_tag="ACFM4S_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6050940.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2977..3417
                     /gene="nadR"
                     /locus_tag="ACFM4S_001268"
     CDS             2977..3417
                     /gene="nadR"
                     /locus_tag="ACFM4S_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6050941.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3532..4032
                     /gene="hpaC"
                     /locus_tag="ACFM4S_001269"
     CDS             3532..4032
                     /gene="hpaC"
                     /locus_tag="ACFM4S_001269"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6050942.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTNGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            4048..4743
                     /gene="murU"
                     /locus_tag="ACFM4S_001270"
     CDS             4048..4743
                     /gene="murU"
                     /locus_tag="ACFM4S_001270"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6050943.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEEALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHHPDGDFLLLPDGSVQSEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4988..5419)
                     /locus_tag="ACFM4S_001271"
     CDS             complement(4988..5419)
                     /locus_tag="ACFM4S_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6050944.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            5594..7270
                     /locus_tag="ACFM4S_001272"
     CDS             5594..7270
                     /locus_tag="ACFM4S_001272"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6050945.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            7384..8475
                     /gene="ychF"
                     /locus_tag="ACFM4S_001273"
     CDS             7384..8475
                     /gene="ychF"
                     /locus_tag="ACFM4S_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6050946.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYEDFIALGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            8513..8842
                     /locus_tag="ACFM4S_001274"
     CDS             8513..8842
                     /locus_tag="ACFM4S_001274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050947.1"
                     /translation="MPSEAGFGGFRRFHTAGMRLHQNKIPSAFPICPPRFLQNKPPAP
                     LLQKAVRHSEYPEPRYKMTFQTAFAPPRFEYSGLTLNQDKATKPQTVQIVRQGEATPY
                     WFKFNPL"
     gene            8850..10197
                     /locus_tag="ACFM4S_001275"
                     /pseudo
     CDS             8850..10197
                     /locus_tag="ACFM4S_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
BASE COUNT         2257 a         2667 c         2881 g         2409 t
ORIGIN      
        1 caagcctttc ggcttgttgc cctctctcta gctctctccc acagggagag aggacgggtc
       61 ggctgttggg gttgaggttt gctgcaagga aaacaggttg tgcaggctgc tttttatttt
      121 tcagacgacc tgaaacaaca gctgcaaatc taagttgcct ttcaaatcgc ctgcaaccga
      181 aaaatctgcc gaaacccact ccctcccccg tgggggaggg ccggggaggc ggcattttcc
      241 aagttgcggc aacctttccc aacaactcaa ccgccccaat acaagccttt cggcttgttg
      301 ccctctctcc agccctctcc cacggggaga gaggacgggg aggctgttgg ggttaagggt
      361 tttgtaaact aatcaggttg tgcaggctgc tttttgcttt tcagacgacc tgcatcaacg
      421 gttgtaaatc caatctgttt ttcaaaccgc ctgcaaccgc aaaacctgcc gcaatccgct
      481 ccctcccctg tgggggaggg ccggggagag ggcattctcc aagttgcggc aatctttccc
      541 aacagcttaa ccgccccaat acaagccttg cggcttgttg ccctctctcc agccctctcc
      601 cacggggaga gaggactatg aagcccgccg gcaggcgcgg ggcttgggac ggcattgctg
      661 ttgcggtttc ggacgcggct ttattcgacg acgattttag ggaaccggct gctgaagtct
      721 ttgcctttgt cggcaatgcc cagggcgatt tggaatgcgg catcggtgta gattcgggcg
      781 atggcggggt gttcgtcgaa cagttgcgcc ggtgtgccgc cgtccatggc ttcgcgcacg
      841 ggcaggctta agggaagctg tccgagcagg gggacgttga ggcgtgccgc caaatctttg
      901 ccgccgtccg tgccgaacag tgcttcgctg tgtccgcagt tggagcagat gtggacggac
      961 atattttcca atacacctaa aatgggaatg ttgacttttc ggaacatatc cacggctttg
     1021 cgcgcgtcta tcagggcgat gtcctgcggc gtggttacga tgaccgatcc ggttacgggg
     1081 atgcgttggg acagcgtgag ttggatgtcg cccgtgccgg ggggcaggtc gataaacagg
     1141 tagtccactt cgtcccactc gctttgaaac atcagttgct gcaaggcttg gctgaccatc
     1201 ggcccgcgcc agacgacggc ttggtcggta tcgacgagaa agccgataga catgacctgt
     1261 atgccgtctg aagattcgac gggaatgagt ttttggtttt tctgatcggg tttgcggtcg
     1321 tccacaccca acatggtcgg ttggctcggg ccgtaaaggt cggcatcgag cacgccgacg
     1381 cgcgcgccca tccgcgccat tgcggcggca aggttggcgg tggttgttga tttgcccacg
     1441 ccgcctttgc cggaggcgac ggcaatgatg tttttcacac ctttgatggt ggtaacgcca
     1501 ggttggactt tatgtgtgcc gatttcggtg tctatggaca gatggatgtg cgcgccgcct
     1561 gtttcgggca tcaggatttc ttgtacggaa tcggcgacgg cggcggcgat gtgcgcgaca
     1621 gggaagccga aatgcagggc gatatggatg ccgtctgaac gctgttcgac cgaacggacg
     1681 gccttttcgc cgccgagcgt gcgtgccgta ttcggaacgg cgacggtgtc gaggagggtg
     1741 cggatgtttt gtatgttcat aatgtgtgtt tctgtgtgca gtttgggaaa cgggcggaaa
     1801 gtgtacattg atttcgggcg gtttgccttt ccttgtgcga acttttacac ggtatgcggc
     1861 ggattgtgaa aatccgttgt caaaatatcc cgtgcaaaca gtggtctgca attaagcctg
     1921 taaaatttgc ttgacgaggc aggatggtgc tgtataatgc acggcttatc gggtcgttag
     1981 ctcagtcggt agagcagcgg acttttaatc cgttggtcga gcgttcgaat cgctcacgac
     2041 ccaccagata accggaagcc aagttttgag acttggcttt ttgtttgcct gccggttgcg
     2101 cgggcgtacc gtttgagaga gcgtatgaaa aagaaactgc tttcgggcat caaatttgcc
     2161 gttcagacgg cattggtgtt tttattggtg tcgctgtttt tggattgggt acgcaagccc
     2221 gaagagccgg ccggtgcggc agggcggcct ttgaccctgc tgtcggggca gcggctgact
     2281 ttgggacagt ttagccggga taaggcggtt ttggtgtatt tttgggggag ctggtgcggc
     2341 gtgtgccgtt atcagtcgcc gataatcgat gatttggcgg cggacggcgt gccggtcgtc
     2401 ggcgtggcgg tacgttccgg cagcgcgtcc gaagtggcgg cgtatatggc aaagcggggc
     2461 ttgggctttc cgactgtcaa cgatgaggac gggggtttgg caaggtcttg gcggattgtc
     2521 gcaacgccag ccgtcgtttt ggtcaaaaat gggaaaatgg ttcactatac gacgggaatc
     2581 agcagttatt ggggcttgcg cgcacgaatt tttcaggcgg atgttttcgg ttaacttttg
     2641 tttttgttaa actttacgct atgtagagaa tcaaacggca tataccttgc tcgtctgatg
     2701 cggcggcttt tgttttttct ggaagaaagt tctttcgaca gtaaatgtaa tactttgttg
     2761 agattgtagg gaaaatagac taagatataa ctattaaaat atttttagat aggattatcg
     2821 gaattaaagt cttttatacc cagtcgtccg atgcggttta tagcgtattg ttgctatatg
     2881 ttcgttttgt tatataacgg ttgcatcaaa atttacgccc acaggttttc ccgacggttt
     2941 gaaagtttga ttttcgataa cttggagact tgaacaatgc ctacccaatc aaaacatgcg
     3001 tctatcaata tcagtctgat acaggcaagg gaagccctga tgacccaatt caggcctatt
     3061 ctgaatcagg cgaatattac cgatcagcaa tggcggatta tccgtctttt ggcggaaaac
     3121 ggcacgctgg actttcaaga tttggcgaat caggcgtgta ttctgcgccc cagcctgacc
     3181 ggtatcctga cccgccttga aaaagcgggt ttggttgtcc gcctgaaacc ttccaacgac
     3241 caacgccgcg tttttctgaa gctgactgcc gagggcgaga agctgtatga ggaaatcggc
     3301 gaagaagtgg acgagcgtta cgacgctatc gaggaagtgc tgggccgcga gaaaatgctg
     3361 ctgcttaaag acctgttggc agaacttgcc aaaatcgagg atgcgttgaa ctcgtaatac
     3421 gccgtaacgc gcggaaatgt ccgaccgacg gctttttgaa tcagaactgc tgcacatggg
     3481 gggtgccttg tgtgcagcat tcttatatag gggacagttt aaaggggaaa aatggcggat
     3541 ttgcagaaaa cttttcaaac ttcgttccgt gatgcgatgg catcctgtgc ggcaggcgtt
     3601 catgtcatca cgacaaacgg tgcggcaggg cgttacggca ttacaatgac ggcggtcgcg
     3661 ccggttaccg acgagccgcc gaccgtgatg ctgtgcatca accggagtgc gcgaatcatt
     3721 ccgatcctgt cggaaaacgg cagcctctgc atcaatatgc tggcggacga acatcaggat
     3781 gttgccgaac attttgccgg gctgaccggc ctgtcgcccg aagagcggtt tgcctaccac
     3841 atctggcatc gcggcaaaac gggacaactt gaaatagaag gcgcgttggc gcacctgcac
     3901 gggcatattg tcggcaaaca tgaaatcggc acgcattttg tgttttacgt caggctcgac
     3961 gaaatcaaaa actgcgggtg caaacgcccc gcgctgctgt atttcagacg gcagtttaga
     4021 tttttagact gatattcgga cagatatatg aaagcgatga tactggcggc aggacgaggc
     4081 gagcgtatgc gccctttgac cgataccact ccgaagccgc tgctcgatgt ggcgggtaag
     4141 cctctaatcg gttggcacct atgccgtctg aagcaggcag ggtttaccga aatcgttatc
     4201 aaccacgctt ggctgggtcg gcagatagaa gaggctttgg gcgacggctc ggcttatggc
     4261 gtgaacatcg cctattcgcc cgaacccgca ggcggtttgg aaacggcagg cggcatcgcg
     4321 caggcattgc cgctgttggg tgggcagccg tttttggtgg tcaacggcga cgtgctgacc
     4381 gacatcgatt ttaccgccgc gtttcaggcg gcatcgtccc tgccggaaca tatttccgcc
     4441 catctgtggc tggtggaaaa tccgccgcac catcctgacg gcgatttttt gctgttgccg
     4501 gacggaagcg tacagtcgga agtatccggc ggcaacggat tgacattcag cggcgtgggt
     4561 atttaccgtc ctgaaatgtt tgacggaatc gaagcgggca gtgtggcgaa actcgcgccc
     4621 gtattgcggg gcgaaatgcg gcaaaaccgc gtgagcggac agaagcatac gggcttgtgg
     4681 ctggatgtcg gcacggtatg ccgtctgaaa gaggctcaag cccttgcagg ggcttggaag
     4741 tgaaataaaa cggtggcttg ccgagtcggc tgccgaacgg ttccgaccgg atgtccggca
     4801 tattttcgtg gaggcgtgaa aatagggcgt tcggggcttt ccgtccaaca aatgccatct
     4861 gaagccgacc ctgtcggatg cggcggtttg ccgtcggcgc ggtgtcggat tttattgggt
     4921 aactttgttc agaccgccgc ccgtcagatt gtcaaaaccc ggtttcaggc ggtatggcgg
     4981 attcggttta acgtttcaac gccagcacca acacgcccgc cgttaccagc cccaagccta
     5041 tccattcctg cgtgttcggg cgttcgtcca agaaaaccac cgccatcagc gcgaccaaga
     5101 ccaggctgaa tttgtcgacg ggggcgactt gcgaggcgtt acccagttgc agggctttaa
     5161 aataggcgag ccaagatgcg ccggtagcaa gaccggatag gataaggaat gtccagttgc
     5221 gccccgtaaa gccgttcaca ccctgccatt tgccggtgta ggtcaaaaac aataacaaag
     5281 cggcgaggat gaccaaggtg cggatgaagg tagcgaaatc tgaatctata ccctgtaaac
     5341 ccattttggc gaagatggcg gtcaatgagg cgaagcctgc cgatgccaat gcccaaaaca
     5401 gccatgcgtt gctgctcatg ttttctcctt ggattgtgaa caatatgaac ggtatttttg
     5461 ttgcagcgtc aaaaatttca ctgcgggttt ggtgcggata acgttataat atgcctgata
     5521 ttattttcaa tccacctgtt tgtcgcctga tgctttcaga cggcatgtcc ctcctcattt
     5581 ctaaaggaaa atcatgagct tcaaaaccga tgccgaaatc gcccaatcct ccaccatgcg
     5641 cccgattggc gaaattgccg ccaagctggg tctgaatgcc gacaacattg agccttacgg
     5701 tcattacaag gcgaaaatca atcctgccga agcgttcaaa ctgccgcaaa aacagggcag
     5761 gctgattttg gttaccgcca ttaatccgac tccggcgggc gaaggcaaaa ccaccgtaac
     5821 catcggtttg gcggacgcgt tgcgccacat cggcaaagat gccgtgattg ccctgcgcga
     5881 accttctctg gggccggtgt tcggcgtgaa aggcggcgcg gcaggcggcg gctatgccca
     5941 agttttgccg atggaagaca tcaacctgca cttcaccgga gattttcacg ccatcggtgc
     6001 ggcaaataat ctgcttgccg cgatgctcga caaccatatc tatcagggca acgagttgga
     6061 catcgacccc aaacgcgtgc tgtggcggcg cgtggtcgat atgaacgacc gccagttgcg
     6121 caacatcatc gacggcatgg gtaaacccgt tgacggtgtg atgcgccccg acggttttga
     6181 tatcaccgtt gcctccgaag tgatggcggt attctgcctt gccaaagaca tcagcgattt
     6241 gaaagagcgt ttgggcaaca tcctcgtcgc ctacgccaaa gacggcagcc ccgtttacgc
     6301 caaagatttg aaggcacacg gcgcgatggc ggtcctgctc aaagatgcga ttaagcccaa
     6361 tttggtgcaa accatcgaag gcacgcccgc cttcgtacac ggcggcccgt tcgccaacat
     6421 cgcccacggc tgcaactccg taaccgcaac ccgtctggcg aaacaccttg ccgattacgc
     6481 cgtaaccgaa gcaggcttcg gcgcggattt gggcgcggaa aaattctgcg acatcaaatg
     6541 ccgccttgcc ggtttgaaac ctgatgcggc ggtcgtcgtg gcgactgtcc gcgctttgaa
     6601 atacaacggc ggcgtggaac gtgccaacct cggtgaagaa aacctcgaag ccttggcaaa
     6661 aggtttgccc aacctgctga aacacatttc caacctgaaa aacgtattcg gactgcccgt
     6721 cgtcgttgcg ctcaaccgct tcgtgtccga ctccgatgcc gagttggcga tgattgaaaa
     6781 agcctgtgcc gaacacggcg ttgaagtttc cttgaccgaa gtgtggggca aaggcggcgt
     6841 gggcggtgca gacttggcgc gcaaagtcgt caacgccatc gaaaaccaac cgaacaactt
     6901 caatttttcc tacgatgttg agctgagtat taaagacaaa atccgtgcga ttgcccaaaa
     6961 agtgtacggc gcggaagatg ttgatttcag cgcggaagcg tctgccgaaa tcgcttcact
     7021 ggaaaaactg ggcttggaca aaatgccgat ctgcatggcg aaaacccaat actctttgag
     7081 tgacaacgcc aaactgttgg gctgccccga aggcttccgc attaccgtgc gcggtatcac
     7141 tgtttccgca ggcgcaggtt tcatcgtcgc cctgtgcggc aacatgatga aaatgcccgg
     7201 cctgcccaaa gttccggctg ccgagaaaat cgatgtggac gaacacggcg tgattcacgg
     7261 cttgttctga gcggtttttg aaaccggatg ccgtctgaag ccgtttcaga cggcattttt
     7321 tcggaacgcg ggcggcggta tgctataatc cgccgttaaa tttctctatt ttcaggaaaa
     7381 aacatgagtt tgaaatgcgg catcgtcggt ttgcccaacg tcggcaaatc caccctcttt
     7441 aacgcgctga cccaatcggg tatcgaagcg gcaaactatc ctttctgtac catcgaaccc
     7501 aacgtcggca tcgtcgaagt gcccgacccg cgtatggctg aattggcaaa aatcgtcaat
     7561 ccgcaaaaaa tgcagcctgc catcgtcgaa tttgtcgata tcgccggttt ggttgcaggc
     7621 gcgagcaaag gcgaaggtct gggcaaccag ttccttgcca acatccgcga gactgacgcg
     7681 attgttaacg tcgtacgctg ctttgacgac gacaacatcg tccacgttgc aggccgcgtc
     7741 gatccgattg ccgacatcga aaccatcggt acagagttgg cgcttgccga cttggcaagt
     7801 gtggaaaaag ccatcgtccg cgaagaaaaa cgcgcccgtt caggcgacaa agacgcgcaa
     7861 aagctggtcg atttgtgcaa aaaactgctg ccgcatctag acgaaggcaa acccgtgcgt
     7921 tccttcggtt tggacgcgga agaacgcgcg atgctcaaac cgctgttcct gctgaccgcc
     7981 aaaccggcga tgtatgtggg caacgtcgcc gaagacggtt ttgaaaacaa tccgcacctc
     8041 gaacgcctga aagaattggc ggcaaaagaa aacgcccccg tcgtcgccgt ttgcgccgca
     8101 atggaaagcg aaatcgccga attggaagac gacgaaaaag ccgagttcct cgccgaaatg
     8161 ggcttggaag aaccgggcct gaaccgcctg atccgcgcgg gctacgacct cttaggcctg
     8221 caaacctact tcaccgccgg cgtgaaagaa gtccgcgctt ggaccatcca caaaggcgac
     8281 accgccccgc aagccgccgg cgtgattcac accgactttg aacgtggctt catccgcgcc
     8341 caagttatct cctacgaaga cttcatcgct ttaggcggcg aagccaaagc caaagaagcc
     8401 ggcaaaatgc gtgtggaagg caaagaatac gtcgtgcagg atggggatgt gatgcacttt
     8461 ttgtttaacg tgtaacccaa atgcggcagg ttttcagacg gctttgccag aaatgccgtc
     8521 tgaagccggt tttggtggtt ttcggcgttt ccataccgcc ggaatgcggc tgcatcaaaa
     8581 taaaatcccg tccgcatttc cgatttgccc tccccgattc ctgcaaaaca aaccgcctgc
     8641 cccgctgttg caaaaagccg tccggcattc cgaatatccc gagccccgat acaaaatgac
     8701 ctttcagacg gcatttgcgc cgccgcgttt cgagtatagt ggattaactt taaatcagga
     8761 caaggcgacg aaaccgcaga cagtacagat agtacggcaa ggcgaggcaa cgccgtactg
     8821 gtttaaattt aatccactat aaaaacatta tgagccaagc cttaccctac cgcccggaca
     8881 tcgacacatt gcgcgccgcc gccgttttgt ccgtcatcgt gttccatatc gaaaaaaatt
     8941 ggctgccggg cggatttctc ggtgtcgata tattctttgt gatttcaggc tttttgatga
     9001 cggagatcct ccttcgcgaa atgtcggggg gggggggggt tttccctgaa ggcattttat
     9061 atccgccgca tcaagcggat tctgcccgca tttttcgccg tattggcggc aacgctggca
     9121 ggcggcttct ttttattcac caaagatgat ttctttcttt tgtggaaatc cgcgctgacc
     9181 gccttgggtt tcgcctctaa cctgtatttt gcaaggggga aggattattt cgatcccgcg
     9241 caggaagaaa agcccctgct gcacatctgg tctttgtcgg tcgaagaaca attttacttt
     9301 gtctttccga tactgctgct gcttgtcgcc cgcaaaagcc tgcgcgtaca gttcggcttc
     9361 cttgccgcac tgtgtgcctt aagccttgcc gcctccttta taccttccgc gctcgataaa
     9421 tattacctgc cccacctgcg cgcctgcgaa ttactgatag gatcgctgac cgccgtgtgg
     9481 atgcggtacc ggcaacagcg gaatctcgcc gtcgggaaac ggtatgccgc cgtcggcgca
     9541 ttgttttccg cgtgcatact gtccgcctgc ctgtttgcct attcggaaca aaccgcctat
     9601 ttcccaggcc ccgccgcttt gattccctgt ctggctgttg ccgcgctgat ttatttcaac
     9661 cattacgaac acccgcttaa aaaatttttc caatggaaaa tcaccgttgc cgccggtttg
     9721 atttcctatt cgctttatct gtggcattgg ccgatattgg cctttatgcg ctatatcggc
     9781 ccggacaacc tgccgcctta ttcgccggcg gcagcgatcg tcctgactct agcgttttcc
     9841 ctgatttctt atcactgcat cgaaaagccg tttaaaaaat ggaaaggttc gttcgcacaa
     9901 tccgttttat ggatttatgc cttgcctatg ctcattttgg gggcgggctc gtttttcgcg
     9961 atgaggctgc cgtttatggc gcaatacgac cgcttggggc tgacgcgttc caacacctcc
    10021 tgccacaaca ataccggcaa acaatgccta tggggggata cggaaaaaca gccggaactg
    10081 ctggttttgg gcgattccca cgccgaccat tacaaaacat tcttcgatgc cgtgggcaaa
    10141 aaagaaaaat ggtccgccac aatggtttcc gccgacgctt gcgcctatgt ggaaggctga
    10201 gacctttgca aaat
//
LOCUS       DAXHMX010000072        10180 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHMX010000072 DAXHMX010000000
VERSION     DAXHMX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.072"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(99..1190)
                     /locus_tag="ACFM4S_001276"
                     /pseudo
     CDS             complement(99..1190)
                     /locus_tag="ACFM4S_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(1320..1868)
                     /locus_tag="ACFM4S_001277"
     CDS             complement(1320..1868)
                     /locus_tag="ACFM4S_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6050948.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            complement(2023..2394)
                     /locus_tag="ACFM4S_001278"
     CDS             complement(2023..2394)
                     /locus_tag="ACFM4S_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6050949.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVQAANPQAALAQYFGRGGR"
     gene            complement(2450..2557)
                     /locus_tag="ACFM4S_001279"
                     /pseudo
     CDS             complement(2450..2557)
                     /locus_tag="ACFM4S_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2689..4380)
                     /gene="dld"
                     /locus_tag="ACFM4S_001280"
     CDS             complement(2689..4380)
                     /gene="dld"
                     /locus_tag="ACFM4S_001280"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6050950.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            complement(4547..4643)
                     /gene="ffs"
                     /locus_tag="ACFM4S_001281"
     ncRNA           complement(4547..4643)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFM4S_001281"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4904..8737
                     /locus_tag="ACFM4S_001282"
     CDS             4904..8737
                     /locus_tag="ACFM4S_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6050951.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAIFD
                     VHTLDSDGLNVVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAISPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIEGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            8813..9814
                     /locus_tag="ACFM4S_001283"
     CDS             8813..9814
                     /locus_tag="ACFM4S_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6050952.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(9876..10058)
                     /locus_tag="ACFM4S_001284"
     CDS             complement(9876..10058)
                     /locus_tag="ACFM4S_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050953.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
BASE COUNT         2303 a         2761 c         2785 g         2331 t
ORIGIN      
        1 gggtttctgt tttttccgat ggattcctgt tgcgttgggg gctggattcc cgctttcgcg
       61 ggaatgacgc ggtgggggtt tcggtttttt ccgcctgttt attttgcggc ttcgattgcc
      121 gctatttctt tgcgtaggtg tttgattgcg ggggttacga tggcgacaaa catcgcttcg
      181 cggacgcgcc tttgggcggg actgcgcatc aggtagcctt ttgcgccgga atgcagtgcg
      241 gcggattggg cggcggcaag tgccagtacg gcggcggctt cgcgcagctt gagggtagca
      301 aggttgtcgg gcgtgccgct ccaagccaag ccggcgagcc gttcggtttc tgcccacgcg
      361 ccgtccaacc ttgctttgag gctgtcgtag ccgtcgtcga gatagctgtt gacttctgca
      421 ttgacgatgt tggcgaggcg gatgatgccg aggctgccgt ctatcacgcc cgcgccgatg
      481 ccgatttgca ggaggataaa gcctgctttg atgctttgga tgtagtcggc aaactgttcg
      541 ggcgcggcga tgatgtcttc gtcggggata aatacgtctt tgaagttcag gctgaaggtg
      601 cgcgtgcctt cgagggcgca aaattcgggg cagttttgca ggcttacgcc ttcccattgt
      661 ccgccggtga taaacataac gtaaccgtcg ccgatttggg cggtattcgc ctagatgtgg
      721 tcttcgccga tgttggacac ccacggcagc gcgccgttga ctgtgtagcc gccctccact
      781 cgctcagctt ggaggttgtg tttttcgatg ccggcaaggt gtttgacggt attggacatg
      841 cccgtacccg ccaatacttt gccttgcagg atgtcggcga ggtatttgtc tttgacggct
      901 tggttgggcg tttggtgcag ataccacgcg caagccgcct gacaccacgc gctgaaggag
      961 gttgcgccgc attctttgcc gatttcgcgc aagacggcga tttgcgttgc caaacccaag
     1021 ccgttgccgc cttcggcttc tgtaccgact gcgccgaatc cgccgattgc gccgagttcg
     1081 cgcataaatg cttcggggta gtatcctttg cggtcgatgt cgtccactat gggtttgagc
     1141 ttggttttga cgagttcggc aacgttggca atcagggttt gggcgttcat ctttgttcct
     1201 taaggtttgc gggggaatcg ggcgcacgcc tgatgcgcgg ctgcttccct gccgtttggc
     1261 ggcttgtttt tgtttgaggg gggttgaaaa accgcccgat attcgggcgg tttgccgtat
     1321 caggcgtaag cctgcaattc ggggttgatt tcggtttgtc cgaggttgtt gacgtagttg
     1381 cacaaagttg ccaaggctac gcccatcacg acttcgactg cctgctgctg gttgtagccc
     1441 gcatcaaaaa atgctttgag ttcctcgtcg gataccgcgc ctttttttgc cattacggct
     1501 tgggtaaagg cggcgagcgc gccgagttta gcatcgtcaa attcgcctgc cgccaaagcg
     1561 cgcgcggctt tgacggattg ttcggaaagg agttttttca gggttgcgag tttggtgtgc
     1621 cctgccacgc agaagccgca ttggttggtg cgggcggcaa taatctggat tacttcgact
     1681 tcgccggcgg tcaggctgtt ggcggcgttg agcttgccga cttcttggta aaacgccaag
     1741 gcttcggggg cgtttgataa tacgccgata aggttgggga taaagccgtt gttttgaagt
     1801 accgcctcga cgcgcgcttt ggcggcttcg ggggcggttt cgagggtgtg tacggttaaa
     1861 cgtgccattt tctttccttg ttcgcaaata ttgggtacgg gcgcatggta tgcatttcgg
     1921 aacggaatag gaaagactga ttggttatgt gctgcaaacg aaaggttata agaaatgccg
     1981 tctgaacatt tttcagacgg catgatggaa aagaaaacgc gcttatcggc ccccgcgccc
     2041 gaaatattgc gccaatgcgg cttgggggtt ggcagcttgg actgtttcgg gcaattcggt
     2101 atagccgcgc gataaaaggt ggcgtgcata aaggctgttg ccctttgcgc cgaaccagac
     2161 ggaaaccgcc aagcccaata cgttaaacgc cgagtctgtg gcgggcgtgt cgccgtaaac
     2221 cagttgggca aacacagcca gcacgaatat cgcgcccgtc agccccagcc ccaaacccca
     2281 cattcttttg aaacacgccc acagcgtgcc gaacaagagc cccggccatg accagccttg
     2341 tttgacggct tggggcggca gggcgggatg ggtgtagatt ttgtatggtt tcatcgtgtt
     2401 tccttttcgg ttgaaaccct gccctttggg aaggtaggat cagactttat agtggattaa
     2461 atttaaacca gtacggcgtt acctcgcctt gccgtactat ctgtactgtc tgcggcttcg
     2521 ttgccttgtc ctgatttaaa tttaatccac tatatttggg aggggcgcgc ccgtgtgtcg
     2581 gcatacggtt tgaaagcgat tacccgacgg ggaacttcaa acccgacaat gccgtctgaa
     2641 cggcgttttg ccttcagacg gcattgcctg ccttcaaagc ggacacgctt attccgccca
     2701 gtttttcttt ttgctggttt tgcctacgcc cgggttgaag ctgttggtcg ggtcaagttt
     2761 gcggtaaaac tgtttgagcg cgggtttggc ttcatacaaa tgaccgacgt tgtgttcggc
     2821 gggatattgc gcgccgcgtt ggtctaaaag atgcagcatt tcgtgttcca acgccatgca
     2881 gtcgttgcct tttttgatga tgtaatcctg atggaaaacg tggcacatga aatgtccgta
     2941 gtagagtttg tggatgattt tattgtcgat ttcgggcggc agtttttcaa accagtcgcg
     3001 gtcgtcgcgg cgcagggcga tgtcaagcgc gaccaagtct tccacttcgt catcatgcac
     3061 ggcgcggtag cggatggcgg cggaggcgac ggcgaaacgg tgcagcatcg cggcttgggt
     3121 ttcttcggcg ttgcactcga aaaacgcgcc gctgtggtgt gcaaaatatt cttttaagaa
     3181 cgcgcgcgcc tcatccacgc ctttcccgcc cattttcaga atcaaatggt gttcgtattt
     3241 gtcgcggtaa tcgcgcatgg atttgggcag gtggtcgggc aggtatttgc tgacgaactg
     3301 catggccttg tcggaaaaat gtttgggcag gaagctggct tttttgccga acctgtccac
     3361 gcgcgccttc aagtcaaata atttcggcag ttggtgcgta ccgaattttt tgatgacgta
     3421 aaacgtgtct ttgccgtaca cgtcggcaat atcgaaagcg tggcggtgga tgtattcgcc
     3481 ggaaacaggc aggctttcaa attcgcccaa ggcggcgcgg cggatgtcgg tcagctcgtt
     3541 gatgtcgttc gtgccgatgt agaacacggc ggtttgtttt tcttgcggga aggtgtccaa
     3601 acggacggca aaaaccatca gtttgcccgc gcagcccgag gcttcgtaat ggcgggcggg
     3661 gtcggcattg aaacgcgcgg cggtcggttc gtccacttgg cggacgtgtt cgcaataggc
     3721 gtggtcgtgt cctttgcccg cgtcttgttt gatgtctttg ttttgataat gatgaccttg
     3781 caggttggtc aggatttctt cgggcgtgtc gcccaagtct atgcccaagt ggttgaccag
     3841 ttccaacctg ccttcttcgt tgatttgggc gaacagtgcc atttcggtgt aggccgggcc
     3901 gcgctgtacc aatgcgccgc cggagttgtt gcacacgccg cccaagacgg acgcaccgat
     3961 gcaggaagag ccgataaccg aatgcggttc gcgtcccaag ggtttcagca gcaattcaag
     4021 ctggttcagg gtcgagccgg gcaggcagac gacttgttcg ttgttgttga tggtttggat
     4081 gatgttcatc cgcatggtgt tcacaatcac gatgtcgcgg tcgtaatcgt tgccgtcggg
     4141 ggtcgaaccg ccggtcagtc cggtattcgc cgcctgcgta atcacaatca cgtctgcttc
     4201 gacgcacgcc tgcagaattt tccacatttc cagaatgctt ccggggcgaa ccaccgccaa
     4261 cgccttaccc tcgccgaagc ggtaaccttg gcggtattgt tcggttttcg cggggtcggt
     4321 aatgatgtat ttttcgccta cggtttgggt cagtcttgac agtaattgtg atgcgctcat
     4381 ggcagtttcc ttaaaattgt cggcaggtgc attgcacatt ggaattgttt tcacattgta
     4441 gttatacgtt atggcaaagt aaagaaaatg ccgtctgaac ggctttcaga cggcaacggt
     4501 gcgatacggg aacgccggaa catcaaagct ccggcgtttc aaatagggcg gcgggccaaa
     4561 cccccggcac tggcgcaccg gagtgggctg ctggcttccg cccctgaccc ggtgttccga
     4621 tttgccatgc ggggagaccc gcctcagaga aacggcatta taacgggttt tctgaaaaac
     4681 tcaaccgttt tgatacggtc atacgccgga aacaccacct aaaatttata tttgataata
     4741 ttgtcaacaa tttctcaaag cgttattttg tttctataag ggtatttcct gtttcggcat
     4801 tgaaaaatat caaaaattga actacattat cgccttttca aactcgcctg aaaccgaatt
     4861 ttcagacggc attcaaataa aaactgccaa gcacggacac accatgacca cgactactgc
     4921 ccctcagcgt attcgagaaa ttccctataa ctatacttct tataccgacc gcgaaatcgt
     4981 catccgcctg ctgggcgacg aagcgtggca gattctgcaa gacttgcgcg gacagcgtaa
     5041 aaccggacgt tcggcacgga tgctgtttga agtgttgggc gatatttggg tagttgtgcg
     5101 caatccatat ctggtcgatg acttgttgga gcacccgaaa cgccgcgccg cgctggtgcg
     5161 ggaaatgcgc caccgtttga acgaaatccg caaacgccgc gacgataatc ggcaagtgga
     5221 tgtattggtt gcggcagcgg aaaaggcagt cgagcgtttt gatagcagtt ttgatgaaac
     5281 cagccaaaaa cgccatcaaa ttttggaacg cttaagcaaa attaccaagc cgcacaatat
     5341 tatgttcgac gggctggcgc gggtaacgca tgttaccgat gcaaccgact ggcgcgtcga
     5401 atatccgttt gtcgtcgtca atcccgacac ggaggcggaa gtcgcgcctt tggtgcgcgc
     5461 cttaattgag ttggacttgg ttattatccc gcgcggcggc ggtacgggtt ataccggcgg
     5521 cgcgattcct ttggacgcaa tgagcgcggt catcaatacg gaaaagctgg acaaacatcg
     5581 cggcgtggaa tttgtcgaac tggcgggctt ggatggcaga catccgatta tccggtgcgg
     5641 cgcgggcgtg gttacgcggc gagtggaaga aaccgcgcat caggcaggtt tggtgttcgc
     5701 cgtcgatccg acttctgccg acgcgtcctg cgtgggcggt aatgtagcga tgaacgcggg
     5761 cggtaaaaaa gccgtgctgt gggggacggc gttggacaac ctcgcctact ggcaaatggt
     5821 caaccctcaa ggcgaatggc tgcgtatcga acgcgtgcgc cacaatttcg gcaaaatcca
     5881 cgacgaagaa accgcaatat ttgacgttca cacactggat tcagacggcc tcaatgtcgt
     5941 caaaaccgaa cgcttggaaa ttcccggtca caaattccgc aaagtcggtt tgggcaaaga
     6001 cgttaccgac aaattcttga gcggcctgcc cggcgtgcag aaagaaggca cggacggcat
     6061 catcaccagc gttgccttcg tgttgcataa aatgccgaaa tacacgcgca ccgtgtgtat
     6121 ggagtttttc ggcacggttg ccaccgccac gccatctatt gtcgaaatcc gcgacttttt
     6181 gcttgcccat gaaagcgtac ggctggcagg tttggaacat ttggactggc gttatgtccg
     6241 cgccgtcggc tacgccacca aagcggcggg caagggacgg ccgaaaatgg ttttgctggc
     6301 ggacgtggtt tcagacgacg aagccgccgt agaggcagcc gccgaacaca tctgcgaact
     6361 tgcccgcgcc cgcgacggcg aaggctttat cgccatatct cccgaagccc gcaaaacctt
     6421 ctggctcgac cgcagccgca ccgccgccat cgccaaacac accaacgcct ttaaaatcaa
     6481 cgaagacgtg gtcatcccac tcgaaaggct cggcgagtat tcagacggca tcgaacgcat
     6541 caacattgag ctttccattc aaaacaaact caaactctgt gccgccttgg agcaatatct
     6601 gtccggcaaa ctccccatcg acaaaatggg aaccgacctg ccgaccgccg aactgttggg
     6661 cgaacgcggc aaacacgccc tggcccacgt ttccgccgtc aaagcgcgtt gggactggtt
     6721 gctcgccaat ctcgacacgc cgcttgccga ctacaaggcc cgctacggcg cagccgtcca
     6781 cgccgcgccc gaagccaaag acaatgaaag ctgctttacc gcattccgcg atttccgcct
     6841 gcgcgtgtct gtcaaagcag acgtaatgaa gccgctttct gaaatcttca gcggcaaaac
     6901 cgacaccaaa atcatcgaag gcttgggcaa aatccacgcc aaaaccgtgc gcggcagagt
     6961 ctttgtcgcc ctgcatatgc acgccggcga cggcaatgtc cacaccaata tccccgtcaa
     7021 ttcagacgat gccgaaatgc ttcagacggc ataccgttct gtggaacgca ttatgaaaat
     7081 cgcccgttcg cttaacggcg tgatttcggg cgaacacggc atcggcatca ccaagctcga
     7141 atttttaagc gatgaagaaa tgcagccgtt ttggaactac aaaaaccaag tcgatcccaa
     7201 acacaccttc aaccgccaca aactgatgaa aggatcggac ttacgcaacg cctacacgcc
     7261 gtcgttcgag cttttgggtg cggaatcgct gattatggaa aaatcagatc tcggcacgat
     7321 tgccgattcc gtcaaagact gcctgcgctg cggcaaatgc aaacccgtct gctctactca
     7381 cgttccgcgt gccaacctgc tgtacagtcc gcgcaacaaa atcctcggcg tgggcttgct
     7441 gaccgaagcc ttcttatacg aagaacaaac ccgccgcggc gtttccgtca aacactttga
     7501 agaactcatg gacatcggcg accactgcac cgtgtgccac cgctgcgtca aaccctgccc
     7561 cgtcaacatc gacttcggcg acgtaaccgt agccgtccgc aactaccttg ccgattccgg
     7621 ccacaaacga tttgcgcctg ccgcagctat gggtatggcg tttttgaacg ccaccggccc
     7681 gaaaaccatc aaagcccttc gcgccgccat gatacagacc ggcttcccag cgcaaaactt
     7741 tgcctacaaa atcggcaagc ttcttccgat cggcacgaaa aagcaaaaag ccgaacccaa
     7801 ggcaaccgtc ggcaaagccc caattaaaga acaggttatc catttcatca accgtccttt
     7861 gccgaaaagc gtacccgcca aaacaccgcg ctccttattg ggcatcgaag acggcaaaag
     7921 catccccatc atccgcaacc ccgccgcgcc cgaagatgcc gaagccgtgt tctacttccc
     7981 gggttgcggt tcggaacgcc tgttcagcca aatcggactt gccgttcaag ccatgctttg
     8041 gcacgtcggc gtacaaaccg tcctgccgcc cggctatatg tgttgcggct atccgcaaga
     8101 cgcgggcggc aacaaggcta aagccgaaga aatgagcacc aacaaccgcg tggctttcca
     8161 ccgtatggca aacaccctca actacctcga cattaagacc gtcgttgtca gttgcggcac
     8221 ttgttacgac cagctcgaaa aataccgctt tgaagaaatc ttccccggct gccgcatcat
     8281 cgacatccac gaatacctgc tcgaaaaagg cgtgaaactc gacggcgtgc aaggtcagca
     8341 atacctctac cacgacccct gccacacccc catcaaaacc atgaacgcca cccaaatggc
     8401 cagcagcctg atggggcaga aagtcgtctt aagcgaccgc tgctgcggcg agtccggtat
     8461 gttcgccgtc aaacgacccg atatcgccac ccaagtcaaa ttccgcaaac aagaggaaat
     8521 cgagaaaaac ctcaaagagc tgccgcaggg tgaacccgtc aaaatgctta cttcctgccc
     8581 agcctgcctg caaggcttga gccgctacac cgacgacaac aatatgcctg ccgactacat
     8641 cgtcatcgaa atggcgaaac acatcctcgg cgaaaactgg ctagacgagt ttgtgaaaaa
     8701 agccaacaac ggcggcgtgg aaaaagtatt gctgtaacaa cggacacgga aatgccgtct
     8761 gaacgccgaa gtcttcagac ggcattgttt aaacccaata cctacccaaa tcatgattga
     8821 cggacaaaca accgaaccca aacaaaaaac ccgaatcatc cttgccccta tgcagggtct
     8881 ggtcgatgac gtgatgcgcg acctgctgac gcgtattggc ggctacgacg aatgcgtcag
     8941 cgaatttgta cgcattaccc ataccgtgca ttcccgatcc atatggttaa aatatgtccc
     9001 cgaaatcgcc aacggaaaca aaacgttttc cggcacgccc tgcaccgtcc aacttttggg
     9061 cagcgatgcg gacaatatgg cggcgaatgc gctggaagcc gtccgcttcg gtgcgaacaa
     9121 aatcgatttg aacttcggct gccccgcgcc caccgtcaac aaacacaaag gcggcgcaat
     9181 ccttttaaaa gagccggaac tgatattcca catcgtcaaa acgctgcgcg gacgtttgcc
     9241 cgcacatatt ccgctcaccg caaaaatgcg gctcggttac gaagacaaaa gccgggcttt
     9301 ggaatgcgcc tgtgcgattg ccgaaggggg cgcatgcgga ctgaccgtac acgcgcgtac
     9361 caaagccgag ggttacgaac cgccggcgca ttgggaatgg ataaggaaaa tccgagacag
     9421 cgtcaatatt cccgttaccg ccaacggcga cgttttcagc ctgcaagact atatcggcat
     9481 caaaacaatc agcggctgca acagcgtgat gctcggtcgc ggcgcggtca tccgccccga
     9541 tttggcgcgg caaatcaagc aatacgagaa cggcgggccg gtcaaagaca cggattttgc
     9601 cgaagtttcc aaatggatac ggcagttttt cgagctgtgc ctgacaaaag aggcaaacaa
     9661 taaatacccg atcgcccgtc ttaagcaatg gttgggcatg atgaaaaaaa cgttcgaccc
     9721 ggcgcaaacg ctgtttgacc gcgtccgaac ggttaaggat gcggatgaag tccgccgaat
     9781 cctgaacgca tttgagcatg agataaatgt ttgactgtct cccatgcttc aaagcaaatg
     9841 ccgtctgaaa atctttcaga cggcatttca tatctttatt gctgcttttc ttccgtatcc
     9901 ggcgtatttt tgtttttcag cttcgcaccc aagcccaaac gtttttcata atccgattgc
     9961 ggggtatcgt cttcctgcat accgaatgcg ccgacattga cccacagcga cagcagcgcg
    10021 acgacaaagg agcaaaagcc gattacatac caaaacattg cccctcccga tttgttaaaa
    10081 tcatatcaaa tacagtgccg aatttatcac aaacgcacgg gcaaatatag tgaattaaat
    10141 ttaaataagg acaaggcggc gagccgaaga cagtacaaat
//
LOCUS       DAXHMX010000073        10022 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHMX010000073 DAXHMX010000000
VERSION     DAXHMX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.073"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(105..1322)
                     /locus_tag="ACFM4S_001285"
     CDS             complement(105..1322)
                     /locus_tag="ACFM4S_001285"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6050954.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1524..2252)
                     /gene="rph"
                     /locus_tag="ACFM4S_001286"
     CDS             complement(1524..2252)
                     /gene="rph"
                     /locus_tag="ACFM4S_001286"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6050955.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2334..2798)
                     /locus_tag="ACFM4S_001287"
     CDS             complement(2334..2798)
                     /locus_tag="ACFM4S_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6050956.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3015..3791
                     /locus_tag="ACFM4S_001288"
     CDS             3015..3791
                     /locus_tag="ACFM4S_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6050957.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4036..4890
                     /locus_tag="ACFM4S_001289"
     CDS             4036..4890
                     /locus_tag="ACFM4S_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6050958.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4931..6139
                     /gene="pncB"
                     /locus_tag="ACFM4S_001290"
     CDS             4931..6139
                     /gene="pncB"
                     /locus_tag="ACFM4S_001290"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6050959.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6231..7949
                     /gene="argS"
                     /locus_tag="ACFM4S_001291"
     CDS             6231..7949
                     /gene="argS"
                     /locus_tag="ACFM4S_001291"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6050960.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERAAALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSNYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLSEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            complement(7837..8310)
                     /locus_tag="ACFM4S_001292"
     CDS             complement(7837..8310)
                     /locus_tag="ACFM4S_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050961.1"
                     /translation="MAERCRPGAKVQHSAAGGAHACRGKENREKRRAETLPEEKADLR
                     GGKRGMRRAESACVGRKGVIIFCLLACLLACLLACLLAWRLFVSHFGRGGGYGVSRRF
                     FQIRIRMARLLSAIGRCGFTLRPTHRCPANPSLVSACRRSASPVAGGCCGLRFRL"
     gene            8503..9921
                     /locus_tag="ACFM4S_001293"
     CDS             8503..9921
                     /locus_tag="ACFM4S_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6050962.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAACAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2294 a         2965 c         2544 g         2219 t
ORIGIN      
        1 tttttgttcc aaagtcaaaa tatgccgtcc gaacattcgg gcggcagaca aaacggcact
       61 gcccgataaa ggcagtgccg ttgtccgttt caaaccgtga aacatcagcc caaattaaag
      121 gctttatgca ataccctggt tgccagttcc atgtattttt catcaatcaa tacggaaact
      181 ttgatttcgg aggtggaaat catttggatg ttgataccct cttcggcgag cgtgcggaag
      241 attttggcgg ctacaccgac gtgcgaacgc atacccaaac cgactgcgga gactttgcat
      301 acggtgtcgt cgccatcaat agaagccgcg ccgatactgt cttggcgttc cgacaggatt
      361 tccaaagtct gcttgtaatc gccgcgcggt acggtaaagg aaaaatcggt tgtgccttcg
      421 ctgccgacat tttggataat catatcgact tcaatgttgg catcggcaac cgcgcccaaa
      481 atctgatagg caacgccggg tttgtcgggt acgccgcgca cgttgatgcg ggcttggttt
      541 ttatcgaatg cgataccggt tacggcagct ctttccatgt tgtcgtcctc ttcaaaggta
      601 attaaggtgc cattgccgcc gtcttgcagg ctgctcagta cgcgcaggcg cactttgtac
      661 tttccggcga attctactga acggatttgc aagactttcg agccgaggct tgccaattcg
      721 atcatttctt caaatgtaac cgtatccatg cggcgcgctt cgggtacgac gcgcggatcg
      781 gtggtgtaca cgccgtccac gtcggtatag atttggcatt cgtccgcttt gagggctgcg
      841 gcaagcgcaa cggcggaagt gtcggaaccg ccgcgtccga gcgtggaaat atcgccttca
      901 ctgctgatgc cttggaagcc ggcaacgatg acgactttgc cggcggtaag gtcggcacgc
      961 attttttcgt catcaatgct ttcgatgcgg gctttggtgt gggcggtatc ggttttgagg
     1021 gcgacctgcc agcctgtgta gcttttggca tccacgccga tgtctttcaa tgccatcgcc
     1081 aaaaggccga tggttacttg ttcgccggtg gacaaaacca cgtccaattc gcgcggatcg
     1141 ggatgctcct gcatttcgtg cgccaacgcg accaggcggt tggtttcacc gctcatggcg
     1201 gatacgacga cgacgatgtc gtgtccttcg gcgcgggctt tggcgacacg tttggccacg
     1261 tttttaatgc gttcgggcga gcctacggat gtgccgccgt atttgtgtac gattaacgcc
     1321 atgtttcgtg ctttcttgtg tgggattggt cgggcagcat ggtttgctgg aaaagcggct
     1381 attattacta ttttttacat gaagttcaag aacggactgc gctttcccgc ctgccgtttg
     1441 acagcggtca gcgaaaaacc tgttctttca gattgttgac aaaatgccgt ctgaacggtt
     1501 ttcagacggc atccggacga caatcaggcg gcggacaacg cattttgctg gtgttgcagc
     1561 agttcgccta tgcctttttg cgccagtgca accagtttgc ccaattcgtc caaactgaac
     1621 ggcgcgtctt ccgccgtacc ttggatttcg atgatttttc ccgatgcggt catgacgata
     1681 ttcacatcac tgtcgcaacc ggagtcttcg ggataatcca aatccaaaag cggcacgccg
     1741 ttcactacgc ctactgacac agcggcaacg gcttcgcgga tggggttttc actcaaaatg
     1801 ccgtctgaaa ccagtttgcc gacggcgatt tgcagcgcga caaacgcacc ggtaatcgaa
     1861 gccgtacgcg taccgccgtc tgcctgaatc acgtcgcagt caatcaagat ttggcgttcg
     1921 ccgagttttt ccatatccac gaccgcgcgc agcgaacgcc cgataaggcg ttggatttct
     1981 tgtgtgcgcc cggactgttt gcccgccgaa gcttcgcggc gcatccggga agcagttgag
     2041 gcaggcagca tcccgtattc cgccgttacc cagccttggt ttttaccgcg cagaaacggc
     2101 gggacgtttt catctatgga agcggtacaa atcactttgg tattgccgca ttcaataagg
     2161 cacgaaccgt ccgtatgcgg caggaaatga ggggtgattt tgatatcgcg caggctgtcg
     2221 gcggcgcgcg agatgcggat gtaatcaggc atactgccct cccgttaaaa acagataaat
     2281 taaaaagcct taaatatgaa aaatcacatt taaggccttc aaactgaaaa tttctacgcc
     2341 tcttcggctt tgctgcggat aatcaaaagc ggcaggtggc tttggcgcat taccgtttcg
     2401 gcaaaactac ccattaaaag gtgcatcagt ccggtacgtc cgtgcgtacc caacaccagc
     2461 aggtcggcac cgttttcatc ggcataatca accaaatcct gcgccatttc acgcgcaccc
     2521 ttattggcaa ccagcaggtg tttgacggta ttttccacac ccagttcccg ggcggtgcgc
     2581 tcggcggcat ccaaaacttc gttgccttgc gcgacggcgg cggcttcgta gctttcgtgt
     2641 tgcaaaaatt cgggggcgag cgccatatat tcggcaggat tggcaacgtg taccaaagtc
     2701 agacgcgcac cgttgacccc ggcaagctcg gcggcatgtt tcagggcatt gatggacgtt
     2761 tcactgccgt caacggcaac aaccaaatgt ttgtacatat cgtattctcc ttttgcaccg
     2821 cctcgcggtg ccctcttgtc ggatgggcgc agggacagtt tgcgctgttt cattatagac
     2881 ccgccgtcgg gctttataca acagccgaac agcccgaccg ctttccagta taatatgccg
     2941 cttccgtgca gtcaggcatt ttttgccggc tttcgttcac tttttgattt gacgcaatct
     3001 tgcaggattc gaccatgtcc gacaacgctt tgacctcttc gcgacgcttc ggcggcatcg
     3061 ccagactcta cggagactct gccttggcgc acttttcaca ggcacacgtc tgcgtagtcg
     3121 gcgtgggcgg tgtcggctcg tgggcggtcg aggctttggc gcggacgggc atcggacgtt
     3181 tgactttgat tgatttggac aacgttgccg aatcgaatgt caaccgccag ctgcacgccc
     3241 tgaccggaga cttcggcaaa gcaaaagtta ccgccttgcg cgaacgcgtc gcccaaatca
     3301 acccagaatg ccaagtgttt gaaattgaag atttcgttac cgaagacaat ttgccggaat
     3361 acttcggaaa aggttttgat ttcgtcatcg acgcgatcga ccaagtgcgc gtcaaagcag
     3421 caatggcggc ttattttgtg gaacgcaaac aaccgtttgt cctcagcggc ggcgcgggcg
     3481 gacaaaaaaa tccggcgtta atccaaaccg ccgatttgag ccgcgtaacc cacgacccgc
     3541 tgcttgccaa cctgcgctac accttgcgga aacgctacgg attcagccgc gatacgaaag
     3601 caaatatgcg cgtgccttgc gtgtattcga ccgaaaatat cgtgccgccg cagtctaggg
     3661 aggcttgttc ggcagatgcc gctccgcaag gcttgtcgtg cgccggctac ggtgcaagca
     3721 tgctcgttac cgcttcgttc gggctatatt gcgcacaggc ggcggtggaa cacatcgcag
     3781 acaaaaaata agcaatgccg tctgaaacag gattcagacg gcatttgaac aaacgcggca
     3841 atcaagctgt cggttcagaa aaaccaacat cggcacgcct aaaaccgtat tcacaacctg
     3901 ctccttttca aaacatttgc atttaaaagc cgttataatg ccgtctgaac atttgcccga
     3961 ccacattata cgtgaatgtc ggcagattgt tttcttttgt aaacttatat taaaatccac
     4021 ttaccgattc acgccatgcc gcccatccct gccccatccg caccatccga gcacactgtc
     4081 gcatgggtat tcggccaacc cgttaccgat ttgccgcagg atttgtttat cccacccgac
     4141 gcgctgaaag tcgtattggg cagttttcaa ggccctttgg atctactgct gtatctgatc
     4201 cgcaagcaga acatcgatgt tctcgatatt ccgatggtga agattaccga gcagtatctg
     4261 cactacatcg cccaaataga agcctatcag tttgatttgg cggcggaata cctgcttatg
     4321 gcggccatgc tgattgaaat caaatcgcgc ctgctgctgc cgcgtaccga aaccgtcgaa
     4381 gacgaagaag ccgacccgcg tgccgagttg gtgcgccgcc tgctggctta cgaacagatg
     4441 aagctggcgg cgcagggttt ggacgcgctg ccccgagccg gacgggattt cgcgtgggct
     4501 tacctgccgc tggaaattgc cgtcgaagcc aagctgcccg aagtctatat taccgacttg
     4561 acgcaagcgt ggctgggtat tttgtctcgg gcaaaacaca cgcgcagcca cgaagtaatc
     4621 aaagaaacca tctccgtgcg cgcgcaaatg acggcaatcc tgcgccgttt gaacggacac
     4681 gggatatgca ggtttcacga cctgttcaat cccgaacagg gcgcggctta cgtggtcgtc
     4741 aacttcatcg cactgttgga gcttgccaaa gaaggtttgg tcggaatcgt acaggaagtc
     4801 ggtttcggag aaatccgaat cagcctcaat catgaggggg cacattcaga cggcatttcc
     4861 ggcacacggg gcgggcgcga tgtgttctaa tacgccccaa gccgccacca aaaatcggga
     4921 gacacgccat atgaccggca tcatacattc gctgcttgac accgacctct acaaattcac
     4981 tatgctgcaa gtggttctgc accagtttcc gcagacgcac agcctttacg aattccgctg
     5041 ccgcaacgcc tcgaccgtct atccgcttgc cgacatcagg gaagacttgg aagccgaact
     5101 cgacgcgctc tgccaactac gcttcaccca cgacgaactc gactacctcc gtagcctgcg
     5161 tttcattaaa agcgactttg tcgattatct cgaactcttc cagctccaac gccgctttgt
     5221 cgaaatcggc acagacgata aagaccgtct gaacatccgc atcgaaggtc cgatgataca
     5281 ggcgatgttt tttgaaatct tcatcctcgc cattgtcaac gaactttact tccgccgcct
     5341 ggaaacccct gcagtcatag aagaaggcga acgccggctt caagccaaag ccgcgcgcct
     5401 caaagaaatc gccgccgcac aaaaccccga cgaaccgccc ttcctgattt ccgacttcgg
     5461 cacgcgccgc cgctacaagc tcgcgtggca ggaacacgtc atccgcaccc tgctcgaagc
     5521 cgccccaagc atcgtgcgcg gcaccagcaa tgtctttctc gccaaaaaac tcggcatcac
     5581 ccccatcggc accatggcgc acgagttcct ccaagccttc caggccctcg acgtacgcct
     5641 gcggaatttc caaaaggccg cgctcgaaag ctgggtgcac gaataccggg gcgatttggg
     5701 cgttgccctg accgacgtgg tcggtatgga tgccttcctg cgcgatttcg acctctattt
     5761 cgccaaactt ttcgacgggc tgcgccacga cagcggcgac ccttacgttt ggggcgacaa
     5821 agcctacgcc cactatcaaa agctcaaaat cgacagccgc accaaaatgc tgaccttctc
     5881 cgacgggctg gacatcgaac gctcttgggc attacaccaa tatttcaaag accgcttcaa
     5941 aaccggcttc ggcatcggca ccaacctgac caacgatatg gggcatacgc ctttgaatat
     6001 cgtcttgaaa ctggtcgaat gcaacgggca gtccgtcgcc aagctgtccg actctccggg
     6061 caaaaccatg accaacaaca gcaccttcct cgcctacctg cgccaagtgt tcgacgtacc
     6121 cgaacccgaa acgccgtaaa ccggcagaaa aagcgcacaa ttcctgtttc tgccgcataa
     6181 aatcttttaa aataccgcct gatttgaatt taaccgaaag accgaacttc atgaacctac
     6241 atcaaaccgt cgaacacgaa gccgccgccg cctttgccgc cgcaggcatc gccggcagcc
     6301 ccgttgtttt gcagccgacc aaaaatgccg aacacggcga tttccaaatc aacggcgtga
     6361 tgggtgcggc gaaaaaagcc aaacaaaacc cgcgcgaact ggcgcaaaag gtcgccgacg
     6421 cattggcagg caacgccgtc atcgaaagcg cggaagtcgc cggtcccggt tttatcaacc
     6481 tgcgcctgcg ccccgaattt ctcgcgcaaa acattcagac ggccttgaac gacgcgcgtt
     6541 tcggcatagc gaaaaccgac aaaccgcaaa ccgtcgtcat cgactattcc tcgcccaatc
     6601 tggcgaagga aatgcatgtc ggtcacctgc gttccagcat catcggcgac agcatttcgc
     6661 gcgtgttggc atttatgggc aacaccgtcg tccgtcaaaa ccacgtcggc gactggggta
     6721 cgcagttcgg catgttggtc gcttatttgg tcgagcagca aaaagacaat gccgcgtttg
     6781 agctggcgga tttggagcag ttttaccgcg ccgccaaagt gcgctttgac gaagatcctg
     6841 ccttcgccga caccgcgcgc gaatacgttg tgaagctgca aggcggcgat gaaaccgttt
     6901 tggcattgtg gaaacagttt gtcgatattt cgctctcgca cgcccaagcc gtttacgaca
     6961 cgctgggctt gaagctgcgc cccgaagacg tggcgggcga atcgaagtac aacgacgatt
     7021 tgcagccagt ggtcgatgat ttggttcaaa aaggtctggc ggtcgaagac gacggcgcga
     7081 aagtcgtatt cttggacgag ttcaaaaaca aagaaggcga acccgccgcg tttatcgtgc
     7141 aaaaacaagg cggcggcttc ctttatgctt ccaccgactt ggcgtgtttg cgctaccgtg
     7201 tcggcacgct tcatgccgac cgcctgctgt acgtcgtcga ccaccgccaa gccctgcact
     7261 ttgagcaact ctttaccact tcccgcaaag caggctatct gccggaaaac gtcggcgcgg
     7321 catttgtcgg cttcggcacg atgatgggca aagacggcaa accgttcaaa acacgcagcg
     7381 gcgacactgt aaaactggtc gatctgctga ccgaagccgt cgaacgcgcc gccgctttgg
     7441 tgaaagagaa aaatcccgaa ttgggcgcgg acgaagccgc caaaatcggc aaaaccgtcg
     7501 gcatcggcgc ggtcaaatac gccgacttga gcaaaaaccg caccagcaat tatgtgttcg
     7561 actgggacgc tatgctttcg tttgaaggca acaccgcccc ctacctgcaa tatgcctaca
     7621 cccgcgtgca aagcgtgttc cgcaaagccg gtgaatggga cgcaaccgca ccgactgttt
     7681 tgagcgaacc gctggaaaaa cagcttgccg ccgagctgct gaaattcgaa gacgtgctgc
     7741 aaagcgtggc ggacacggcg tatccgcact acctcgccgc ctacctctat caaattgcga
     7801 ccctgttcag ccgcttctac gaagcctgtc cgatactcaa agccgaaagc gcaagccgca
     7861 acagccgcct gcaactggcg aagctgaccg gcgacacgct gaaacaaggc ttggatttgc
     7921 tgggcatcga tgtgttggac gtaatgtaaa accgcaccgc ccgattgcgg acaacagcct
     7981 cgccatcctt atccgaatct gaaaaaagcg gcgcgataca ccgtatccgc cgcccctccc
     8041 aaaatgcgaa acaaacaaac gccaagcaag caagcaagca agcaagcaag caagcaagca
     8101 agcaagcaag caaaaaatta taaccccctt cctgccgacg cacgcacttt ccgcgcggcg
     8161 cattcccctt ttcccgcccc tcaaatccgc cttttcttca ggcagggttt cagcccgcct
     8221 cttttccctg ttttcctttc cccgacacgc gtgcgcgccc cctgccgcac tgtgctgcac
     8281 tttcgcgccc ggacggcatc gttccgccat ccggttctct gttttaaata cccctgtttc
     8341 agaaagaaat gcagatgttt caacacacag gacgacacat aaagcaccgc cctatgtgtt
     8401 gccctgattt ggaaggggtt acgcctccca aataaagtct gatcctgccg ccccgaagga
     8461 cagatgtccg agtggcgaag tttcaaccga aaaggaaata cgatgaatat tcacaccctg
     8521 ctctccaaac aatggacgct gccgccattc ctgccgaaac ggctgctgct gtccctgctg
     8581 atactgctgg cccccaatgc aatgttttgg gttttggcac tgctgaccgc caccgcccgc
     8641 ccgattgtca atttggacta tcttcccgcc gcgctgctga tcgccctgcc ttggcgtttc
     8701 gtcaaaattg ccggcgtatt ggcgttttgg ctggcggttt tgtttgacgg gctgatgatg
     8761 gtgatccagc tcttcccttt tatggacctc atcggcgcca tcaacctcgt ccccttcatc
     8821 ctgaccgccc ccgcccctta tcagataatg accgggctgt tgctgctgta tatgctggcg
     8881 atgccgtttg tgttgcagaa agccgccgcc aaaaccgact tccgacacat tgccgcctgt
     8941 gccgccgttg tggcggcagc cggctatttc actggccatt tgagttacta cgaccggggg
     9001 cggatggcca atatcttcgg cgcaaacaac ttctactacg ccaaaagtca ggcgatgctc
     9061 tacaccgtca gccagaatgc cgactttatt accgccggcc tggtcgatcc cgtcttcctc
     9121 cccttgggca atcaacagcg tgccgccacg catctgaacg agccgaaatc tcaaaaaatc
     9181 ctctttatcg tcgccgaatc ttgggggctg ccggccaatc ccgaacttca aaacgccact
     9241 tttgccaaac tgctggcgca aaaagaccgt ttttcggttt gggaaagcgg cagttttccc
     9301 tttatcggcg cgacggtcga aggcgaaatg cgcgaactgt gtgcctacgg cggtttgcgc
     9361 gggttcgcac tgcgccgcgc gcccgacgaa aaatttgccc gctgcctccc caaccgtttg
     9421 aaacaagaag gttacgccac ctttgcgatg cacggcgcgg gcagttcgct ttacgaccgc
     9481 ttcagctggt atccgagggc gggctttcaa gaaatcaaaa ccgccgaaaa cctgatcggt
     9541 aaaaaaacct gcgccatttt cggcggcgtg tgcgacagcg agctgttcgg cgaagtgtcg
     9601 gcatttttca aaaaacacga caagggactg ttttactgga tgacgctgac cagccacgcc
     9661 gactatcccg aatccgacat tttcaaccac aggctcaaat gcaccgaata cggcctgccc
     9721 gccgaaaccg acctctgccg caatttcagc ctgcacaccc aattcttcga ccaactggcg
     9781 gatttgatcc aacgccccga aatgaaaggc acggaagtca tcatcgtcgg cgaccatccg
     9841 ccgcccgtcg gcaacctcaa tgaaaccttc cgctacctca aacaggggca cgtcgcctgg
     9901 ctgcacttca aaatcaaata acaacaatgc cgtctgaacg caccaacagc cttcagacgg
     9961 cattttgcag acagaccgac ccttcaagcc cacttttttc atcatctccg ataaattgct
    10021 tt
//
LOCUS       DAXHMX010000074         9982 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHMX010000074 DAXHMX010000000
VERSION     DAXHMX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.074"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..872)
                     /locus_tag="ACFM4S_001294"
                     /pseudo
     CDS             complement(1..872)
                     /locus_tag="ACFM4S_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1470..3050)
                     /gene="purH"
                     /locus_tag="ACFM4S_001295"
     CDS             complement(1470..3050)
                     /gene="purH"
                     /locus_tag="ACFM4S_001295"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6050963.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDILSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
     gene            complement(3211..4434)
                     /locus_tag="ACFM4S_001296"
     CDS             complement(3211..4434)
                     /locus_tag="ACFM4S_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6050964.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            complement(4498..5331)
                     /gene="serB"
                     /locus_tag="ACFM4S_001297"
     CDS             complement(4498..5331)
                     /gene="serB"
                     /locus_tag="ACFM4S_001297"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6050965.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKVQAAADACINFGGLERVRGLF
                     G"
     gene            5621..7162
                     /locus_tag="ACFM4S_001298"
     CDS             5621..7162
                     /locus_tag="ACFM4S_001298"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6050966.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFKKG"
     gene            7166..7549
                     /locus_tag="ACFM4S_001299"
     CDS             7166..7549
                     /locus_tag="ACFM4S_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6050967.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKTGGFRFSDNHHPRDFLARTQ
                     GAAARAHAAQQNSFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            7615..9000
                     /gene="pntB"
                     /locus_tag="ACFM4S_001300"
     CDS             7615..9000
                     /gene="pntB"
                     /locus_tag="ACFM4S_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6050968.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(9208..9651)
                     /locus_tag="ACFM4S_001301"
                     /pseudo
     CDS             complement(9208..9651)
                     /locus_tag="ACFM4S_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
BASE COUNT         2187 a         2777 c         2704 g         2314 t
ORIGIN      
        1 cgcatacatg ccgtaacggc aaccttatac ggcttaccct cggacggcag gcgttggtag
       61 aaatcccgaa taagcggttc aaaacgtgtc gctgccacgg tagccatata cagcgcctta
      121 cgtaccgcag actttccgcc aaagcagcgg cttttgaatt tggtttcccc gctctccctc
      181 gggtgcgggg caatgccggc caaactcgct atccgtttgt gcgacagccg ccccaattcg
      241 ggcagcatcg ccatcagcgt agccgtcgtt atcgaaccga tgcctttgat ttgctccgcc
      301 acttgggctt tgccgtcaaa atgcgtgtgg gtgtggtcgt cgatttgttt gtccaattcg
      361 tcaatcagcc ggtcaaaatg ggcaatcagt tgtttgacgc ttccgacttg cgtttcatga
      421 acctaatgca gacggttttt ctcggcagtc cgcatatccg ccgattggtt gcggcggtta
      481 accaaggctt ccaacacttc ttccgcttcg gtgggcggtt ggtagggcat ggtttgcgaa
      541 tcttctttct gtgccgtcat ctgtgcgaag aaggcgggca ttttggcatc tttggcgtcg
      601 gttttggtca gcggctgcga ttgggcaaac tgatgcgtct gacgcgggtt ggcgataatc
      661 acggctatgc ctgctcggcg gatggctttg gcggcgggga tttcgagacc gccggtactt
      721 tccgtcacga cgagggcgac cttgtgtttt ttaaggtatt cgatagtatg ggcgatacct
      781 ttggggttgt tggtttcggt tttggtttta gacaaagacg aaacggcgat gacgaagttt
      841 cgtttggcga tgtcgatata gtgaattaac aaaaatcagg acaaggcggc gagccgcaga
      901 cagtacggat agtacggaac cgactcactt ggtgcttcag caccttagag aatcgttctc
      961 tttgagctaa ggcgaaccaa cgctgtactg gtttagattt aattcactat acctgcgtaa
     1021 tggtattggg tactcatcat aaacctgcct tgcattcggt tgtgttgtcc ggcaactgtc
     1081 cggttgtgtc gatgggttgc cctgccgctc cctgagctac gcaacggttg tgtgccttgg
     1141 gtgggcgcgg gtggcgctat gatacggtga ttccaatata caagcggtgg gctgaagccc
     1201 accgcttccg tcgattccgt caatgttacc catttccacc gtccccgccg aaaccaaaac
     1261 cgccgactcc cgccgattct ccaaaaattt ttgatgcagc gggctgaagc acaccctgca
     1321 tcccaccttt tacgaatcct cctacaccct atacaacacc ttgaaaatcc accctgtcag
     1381 gaatacccga accgtcatcc ctaccttcac aaaatagcgc aaaatgccgt ctgaaagccc
     1441 ttcagacggc attgccttgt tcatctgcgt taatggcgga agtggcgcac gtcggttacg
     1501 accatggcga tgccgtgttc gtccgctgcg tcgaagactt cctgatcgcg catcgaacct
     1561 gccggatgga tgatggcttt gatgccctgc tcggcaatca cgtccacgcc gtcgcggaag
     1621 gggaagaaag catcagaggc ggcacatgcg ccgtttaagt ccaagcctgc gtcctgcgct
     1681 ttgcgggcgg cgatgcgggt actgtccacg cggctcattt ggcctgcgcc gatgccgtag
     1741 gtttgaccgc ctttgccgaa gacgatggcg ttggatttga cgtattttgc gacgttccaa
     1801 acaaacatca ggtcgttcca ttcttgctcg gtcggttggc gtttggagac gactttcaaa
     1861 tcggcgcggc tgatgcggca gatgtcgggc gtttgtacca acagtccgcc gccgacgcgt
     1921 ttgagttcga agcggtttgc gcctgcctca agcggcactt ccaatacgcg cacgtttttc
     1981 ttggcggcgg cgatttcgag ggcttcggcg gtaaacttcg gcgccatcaa gacttccata
     2041 aactggttgt cggtaatctg tttgacggtt tcgccgtcca cttcgcggtt gaaggcgatg
     2101 atgccgccga acgcgctggt ggtgtcggtg acgtaggcga gtttgtaggc ggtcaatgta
     2161 tcggctgcaa cggcgacgcc gcacggattg gcgtgtttga cgatcacgca ggctggcgcg
     2221 tcaaaggatt tgacggcttc ccatgcggca tcggcatcgg cgatgttgtt gtaagacaat
     2281 tctttgcctt gcagttgttt gtaggcggaa aggctgcctg cggcggggtc aatatcgcgg
     2341 tagaacgcgg cgcgctgatg cgggttttcg ccgtagcgca tgtcttgcac tttaatccag
     2401 ctttggttga actggctcgg gaattcaccg atttcgggcg tgccgctcaa gatgtcgtct
     2461 gaaagcgagg tcaggtagtt ggaaatcatg ccgtcgtatt gggcggtgtg gctgaacgct
     2521 ttgcgcgaca ggttgaagcg ggttttgtcg ctcaacgcgc cgttgttggc ttcgagttcg
     2581 gcggcgatgg cggggaagtc ggcggtgtcg gtaacgatgg cgacgtgttt ccagtttttt
     2641 gcggcagaac gcaccatggt cgggccgccg atgtcgatgt tttcaatcgc gtcttccagt
     2701 gtgcagtttg gtttggcgat ggtggcggcg aagggataaa ggttgacgca cacaaggtcg
     2761 atattgccga tgccgtgttc ttccatcttg gcgacgtgtt cgcccaaatc gcgccgaccc
     2821 aaaataccgc cgtggatttt cggatgcagc gttttcacgc ggccgtcgag catttcgggg
     2881 aagccggtat agtcggcgac ttcaatcacg ggaacgcccg catcggcaag cagtttcgcc
     2941 gtgccgccgg tggagaggat ttcgacaccg agcttggtca gggtttgggc aaattcgacc
     3001 gcgcctgcct tgtcggacag gctgatcagg gcgcgtttga tgacggacat tggggcttcc
     3061 ttttctgaca gttggatggg gaaaggggaa ctttttcggg gcggtattat ccgctatttc
     3121 tagttttttg gcagtaggtt tttgcagata ttgctgacaa tttacaaaga aatgccgtct
     3181 gaaaaccttt cagacggcat ttgtcggcat ttatttgaag cccatttttc ccgatgttcc
     3241 caatgccccc gccatgccga tgcacaaagg cagcagcagg gacagcggcg cgtaggtcag
     3301 ttccaatacg aacacaatgg cggtcagcgg cattttgagc gatacgccga gaaacaccgc
     3361 cgctccgacg actgccgcgc ttccagacgg catttcgggg aaaacgctgt tccacgcggc
     3421 ggcggctgca aaagaaatgg tgctgccgag catcatagac ggcgtaatca gtccgccgta
     3481 tgcgcctgcc gccaacgcca tcaagacaac cagccatttg acggcggtca gctcaaggct
     3541 gtgttgccaa ccggtcaaac cgccaaaggt cagttggttg cctgctttgc cgttgcccaa
     3601 aatttctgga aaccaaacgg caatcacgcc gatgagcgca aacatgcaga ctgccaaggg
     3661 aatgattttg atgttgtcgc gcttgatgaa ggggaatttt tgggcggtgc gctggaaaaa
     3721 gacggcggtt gcgcccagta tcgggccgat gacggcggaa aaccaaagta atgaagtgtt
     3781 tacggcaagg ttggcgggat gatattgctg cacgtcgcct aagccgatgc gcgcgacggc
     3841 ggtggcgatg acggaagtca gcaatgcggc ggcgacggct tgctgcgtcc acacgcccag
     3901 catggcttcg aggataaaaa gtgtggaggc gagcggcacg ttatacacgg ccgccaaacc
     3961 cgcacccgaa gcgcaagcaa tcagcagccg catttcgtct tcatccaaac ccaaccgcct
     4021 gccgccggca aaagcgaacg cggcagtcat ttcacgcggg gcgacttccc gtccgagcgg
     4081 tgaaccgagt ccgaccgtta tgatttgcag cagaacatgg aaaaccgtcg tcagaaacgg
     4141 cagcccctgc aacggctgtt tcaaggcggc tttgatttcg atttgcggct tgccgaaacg
     4201 tttcagcaac caccagccgc tgcctgcgac cgcgccgcac agcgtcagca cggcaacgcg
     4261 ccgcataccg gaagcctgtg ccacaccttc gcggaacgag atggacacgc cgtccgtgcc
     4321 ataaccgtat gccgtatgtt gtatgaagtg catcagttcc gtcagcacaa tgccgaccaa
     4381 accgccgata acgcccgctg ctgccagggc aaaccaaagt tttctgcgtc ccactgtcgt
     4441 tcctgccgtt caaatgccgt ctgaaaacct ttcagacggc atccgtttcc tatccgccta
     4501 tccgaacagg ccgcgtacac gctccaaacc gccgaagttg atacaggcat cggcggctgc
     4561 ctgcactttc ggtttggcac ggtaagccac gcctatgccc gcttctttga gcatcggaat
     4621 atcgttcgca ccgtcgccca tcgccaacac ctgatgcggc tgcaatccga ggcggctgcg
     4681 gtattcgcgc aataaatccg cctttgcctg cgcgtcgatg attctgcctt tcagacgacc
     4741 ggtcaacctg ccgttttcaa tttccaaaac attggcgtgt tggtattcga agccgagtcg
     4801 ttgttgcagc ctttcggtaa aaaatgtgaa cccgccggac accagcagga atttcacatc
     4861 gtgccttttg cattcgtcca acaagaattc cgcaccgggc gagagcttca aaacgttttc
     4921 ataaacgtcc gccaaaatct gttcgtccaa tcccgccaac agcgcaacgc ggctgcgtaa
     4981 agactgttcg aaatcgagtt cgccgcgcat cgaacgctcg gtaatttccg ctactttgtc
     5041 ttttaaaccc acgcctgccg caatttcatc gacgcattca atggtaatca gcgtcgaatc
     5101 catatcgctg acaattaaac cgagttcgtc gaaatccata tccggcaaca cggcgtggtc
     5161 gatttgacgg ctgccaagca acgccgcgtc cgcctcgctt aaagaaaacc cttcttcaac
     5221 gataaaacgc atacgctttt catcggcgta atcaggttcg ggcaggcggg aggggaagtc
     5281 ggaaggcagg gcttcggcgg agggaaattg gaggacgagg gcgtgcggca tggcggtaac
     5341 tcaaaaaatg cggattatat agtgaaacgg caaagaatgt tacggcgggc gggactgcaa
     5401 tcggaaaatg acttcaaacg caaacggcag tatgtgtcgg acaacacggg aaaatgccgc
     5461 aactattgcc agcctgatga aaattcgtta taaggggatt atctaaaata tattaacatt
     5521 tgaagtgaat cggttttaaa ccggtacggc gttgctccgc cctgccccga tttaaattta
     5581 acccacgata catataaaca acccgaaaaa ggattcagag atgaaaatcg gcatcccacg
     5641 cgagtcatta tccggcgaaa cccgcgtcgc ctgtacgccc gccaccgtcg ccctgctggg
     5701 caaactgggc tttgaaaccg ttgtcgaaag cggtgcaggt ttggcggcaa gtttggacga
     5761 tgccgcttac caaacagcag gcgcaaccgt tgccgacaaa gcggcggttt gggcctgccc
     5821 tttgatttat aaggttaacg cgccgtccga aggcgagctg ccgcttttga acgaaggtca
     5881 aaccatcgtc agcttcctgt ggccgcgcca aaacgaggct ttggtcgaag ccttgcgcgc
     5941 caagaaagtc aatgcgctgg cgatggacat ggtgccccgt atttcccgcg cgcaggcttt
     6001 ggacgctttg tcttcgatgg caaacatcag cggctaccgc gccgtaattg aagccgccaa
     6061 cgccttcggc cgtttcttca ccggtcaaat caccgccgcc ggcaaagtgc cgcccgcgca
     6121 ggttttggtg attggtgcag gtgtggcagg tttggcggcg atcggtacgg caaactcgct
     6181 cggcgcagtg gtacgcgcgt tcgatacccg cttggaagtg gcggaacaaa tcgaatcgat
     6241 gggcggcaag ttcctgaaac tcgacttccc acaagaatcg ggcggcagcg gagacggcta
     6301 cgccaaagtg atgagcgacg aatttatcgc agccgagatg aagctctttg ccgagcaggc
     6361 gaaagaagtg gacatcatca tcaccaccgc cgccattccg ggcaaacccg cgcccaagct
     6421 gattaccaaa gaaatggtgg aaagcatgaa atccggctcc gtcatcgtcg atttggcggc
     6481 ggcgacgggc ggcaactgcg aactcacccg cccgggcgaa ttgtccgtaa ccggcaacgg
     6541 cgtgaaaatc atcggctaca ccgacatggc aaaccgcctt gccggacagt cttcccagct
     6601 ttacgccacc aacttggtca acctgaccaa gctgttaagc ccgaacaaag acggcgaaat
     6661 cacgttggac ttcgaagacg tgattatccg caacatgacc gttacccacg acggcgaaat
     6721 caccttcccg cctccgccga ttcaagtttc cgcccagccg cagcaaacgc cgtctgaaaa
     6781 agccgcgcct gccgccaagc ccgagccgaa acctgtttcc ctgtggaaaa aactcgcgcc
     6841 cgccgtcatc gccgccgtat tggtgctgtg ggtcggcgcg gtcgcacccg cagcattcct
     6901 gaaccacttt atcgtgttcg tcctcgcctg cgtcatcggg tactacgtcg tttggaacgt
     6961 cagccactcg ctgcacacac cgctgatgtc ggtaaccaac gccatctccg gcatcatcgt
     7021 cgtcggcgcg ctgctgcaaa tcagccaagg caacggtttc gttaccctgc ttgccttcat
     7081 cgccatcctg attgccagca tcaatatctt cggcggcttc ttcgtaacgc gccgcatgtt
     7141 gaatatgttt aagaaagggt aagccatgac tttcgcctat tggtgcatcc tgattgcctg
     7201 cttattgccg cttttttgtg cagcgtatgc caaaaaaacc ggcggattcc gcttcagcga
     7261 caatcatcat ccccgtgatt ttctggcgcg tacccaaggt gccgctgccc gtgcccatgc
     7321 cgcgcagcaa aacagttttg aagcatttgc accatttgca gccgccgttt tgacggcaca
     7381 cgcaaccggc aatgccggac aagcaaccgt caacacgctt gccggcctgt tcatcctgtt
     7441 ccgcctcgcc tttatctggt gctacatcgc agacaaagca gcattgcgct cgctgatgtg
     7501 ggcgggcgga tttgcctgca ccgtcgggct gtttgtcgcg gctgcttgaa acagatgccg
     7561 tctgaacagt tcagacggca ttaaaaacaa atcatcgaaa atcggagaat ttccatgtct
     7621 tcaggactcg taacagcggc gtatatcgtt gccgcaattt tattcatctt ctcactggcg
     7681 gggctgtcca aacaggaaac cgccaaacag ggctgctatt ccggtatcgc cggtatggcg
     7741 gtcgcccttt ttgtaactgt tttttccgac aatacccacg gactgggctg gatcatcatc
     7801 gccatgctca tcggcgcggc aatcggcatc tacaaagcca aaaaggtgga aatgaccgaa
     7861 atgcccgaac tgattgccct gctgcacagc ttcgtcggcc tagcggcggt tttggtcggc
     7921 ttcaacagct atatcgagcc gggcaacgtt tcgcacgata tgcacaccat ccatctggtc
     7981 gaagtctatt tgggcatttt catcggcgcg gtaaccttta ccggctcgct ggtcgcattc
     8041 ggcaaactca acggcaaaat cagcagcagc ccgctgcaac tgcccgccaa acacaagctc
     8101 aacgcactgg cgctcgccgt atcgtttgtg ttgctgctcg tattcgtcgg cattaacggt
     8161 agcggcttca tcctgctgat tatgaccctg attgccctcg cattcggctg gcacttggtt
     8221 gcctccatcg gcggcgcaga tatgcccgtg gtcgtgtcca tgctcaactc ctactccggc
     8281 tgggcggccg cagcggcagg cttcatgctc tccaacgacc tgctcatcgt taccggcgcg
     8341 ctggtcggct caagcggcgc gattctgtcc tacattatgt gcaaagccat gaaccgctcg
     8401 tttgtctcgg taattgccgg cggtttcggc agcgacagcg gcacattatc ttccggcagc
     8461 caagagatag gggaataccg agaagtcaaa gctgccgata ttgccgaaat gctgaaaggc
     8521 gcaaacaatg tcatcattac cccgggctac ggtatggcag tcgcacaagc gcaatacccc
     8581 gttgccgaaa tcaccgagct tttacgtaaa aacggcaccg aagtacgctt cggcatccac
     8641 cccgtcgccg gccgcctgcc cggtcatatg aacgtactgc tcgccgaagc caaagtcccc
     8701 tacgacatcg ttttggaaat ggacgaaatc aacgacgact tccccgaaac cgatgtggtc
     8761 ttggtcatcg gtgcgaacga caccgtcaac cccgccgccc aaaccgaccc gaacagcccg
     8821 attgcgggta tgcccgtgtt ggaagtgtgg aaggcaaaag aagtcgtcgt cttcaaacgc
     8881 tcgatgaata ccggctacgc aggtgtacaa aacccactgt tcttcaacga aaacagcgtg
     8941 atgtgtttcg gagatgcgaa gaaaaccgta gatgacattc tgtccgaact gaaaaaataa
     9001 tgccgtctga acaattcggc gcaggttttc aatctgtttg atttgaaaaa atcactgcaa
     9061 accccgttta tgaaggtttg cagtgatttt tttgcattgg ggcaaacatt ttcagacggc
     9121 ccgtccgaat aagcacctac atctgaaact gacacaaaat caacgaaaca aacaaaatcc
     9181 atctccgtgt tgaagatgga tttttaatta gccgaacact ttttccaagt gtgcctgata
     9241 atctgccaag tatttttcca cttgcggatt tttaaccaca tcgttacata agaatgtcgg
     9301 caggcgggtc atacccaaaa actcgttggc tttgtggaag tgcatataca aaacatcaac
     9361 gcctttgcct tcaaagaaat cgccttcgcg ggtaaacgcc tcaatcggcg cattccaagt
     9421 cagtgaaagc atatgttttt tgccttgcaa caagccgcct gtgccgtagc cctcagtcgg
     9481 attgacgctg tgtctgccgt cgctttggta gagtttgccg tgtccagcgg ttaatactcc
     9541 gtctatgtat tttttcactg tccaaggctc gtgcatccac cagcccggca tctgccaaat
     9601 cacagcatcc atccaaacga atttttcgat ttctgcctca acatcatagc cgggaatcgt
     9661 cagaaatacc tgcatcgtca ttcccgcgca ggcgggaatc cagacctgtc ggtgcggaaa
     9721 cttatcgggt aaaacggttt ctggagattt ttcgtcctgg attcccactt tcgtgggaat
     9781 gacggaatgt aggttcgtgg gaatgacgtg gtgcaggttt ccgtacggat ggattcgtca
     9841 ttcccgcgca ggcgggaatc caattcattc ggtttcagcc gtttccgata aatcactgta
     9901 acgttaagtt cccggattcc cactttcgtg ggaatgacgg cgtaaaggtt tctgcttttt
     9961 aagttttagg taactaatga gt
//
LOCUS       DAXHMX010000075         9957 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHMX010000075 DAXHMX010000000
VERSION     DAXHMX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.075"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(305..4474)
                     /locus_tag="ACFM4S_001302"
     CDS             complement(305..4474)
                     /locus_tag="ACFM4S_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6050969.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLASTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVANAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVIVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
     gene            complement(4529..6376)
                     /locus_tag="ACFM4S_001303"
     CDS             complement(4529..6376)
                     /locus_tag="ACFM4S_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6050970.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6752..7981
                     /gene="sstT"
                     /locus_tag="ACFM4S_001304"
     CDS             6752..7981
                     /gene="sstT"
                     /locus_tag="ACFM4S_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6050971.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8093..9586
                     /locus_tag="ACFM4S_001305"
     CDS             8093..9586
                     /locus_tag="ACFM4S_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6050972.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGTNKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLPGGSYALRVQGEQAKGEMLAGTAVYNGE
                     VLHFHTENGRPSPFRGRFAAKVDFGSKSVDGIIDSGDDLHMGKQKFKAAIDGNGFKGN
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9661..9876)
                     /locus_tag="ACFM4S_001306"
     CDS             complement(9661..9876)
                     /locus_tag="ACFM4S_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050973.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9869..>9957)
                     /locus_tag="ACFM4S_001307"
     CDS             complement(9869..>9957)
                     /locus_tag="ACFM4S_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6050974.1"
                     /translation="VTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT         2024 a         2536 c         3091 g         2306 t
ORIGIN      
        1 tcgggcattc cttaaattac ctgtgtatcg ctgtaaatct tagagatggc ggaatatagc
       61 ggattaacaa aaaccggtac ggcgttgcct cgccttggct caaagagaac gattctctaa
      121 ggtgctcaag caccgagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc
      181 cttatcctga tttttgttaa ttcactatat cgattctgtt cccttgcagg cggacggcgg
      241 cggcggacgg tggaaaagca ggttttcaaa tgtccggttt ggcggcgcgc cgtctgaaaa
      301 ccgcttattt tcctttgccg tttccggcgg agtctttttt gtccgaaccg gagaagcggt
      361 cgaaacgtat ggtgtatgtc agctcgccgc ccgacgaacg gctgccgata cgggcaaccg
      421 cctgtatggc gcgggtcagc cggtaaatca gtttgacgga ctgttccgcg ctggagatgc
      481 tgtattcgta gccgatgtag agtttgccgg tcagttgttt gccgacggtc agcacctgtt
      541 cggcggggtt gagttcgccg gtttgcgcgt tgcggctgcg cttgctggta aagcccaaat
      601 catccaccag cccgatgcgg tcgttgattt gcccggcaag cagcgcgcct gcggctgcag
      661 acagggcggc attgtcgccg ctgctgccgc tgccggcgcg gttgaggatg agccaagaga
      721 gcttgtcttt ttcactcatc ggttcgtttg ccgtcagcgt aatgcgcggg ctgttgaggc
      781 tgcccaatat ttccacgccc gcaccgacgg gggaaaggcg gcgttcggcg cggatgttga
      841 ggttggggtc gttgagcggg ccgacaaagg agaccgtgcc tttggtaatg tccaaatcct
      901 gcccgtatgc cttataacgc cctttgatga cgcggaccgt gcccacgccc cgcacgcttc
      961 cgcccgattg ggcggtcagg gtcagtttgc cgcctatggt aacgtccgcg ccgtagccgg
     1021 cgaagcggat gccgtcattg aggtctaaag tcaggttcat attgacgggg agcggtgccg
     1081 ccgcctcttt tttgacttcg cctaatacga tgacatcgtc gccgacggac ggcatcgagg
     1141 atttttgcga accgaacagc ccctgatccg ttttaatcat cccggtaacg gatatgcctt
     1201 tttgcggcga atagcgcagg cgggtgttgc cggaaaccgt caggcggcgg ttggggcggg
     1261 acaggatgcg gtatttgtcg aacaccgcgc cgatatcgac atcgggtccg ctgttttcca
     1321 taccgaccgt accggagagt tccgccgtcc cttcgtgacg gaatttcagg ctgtcgatta
     1381 cccatttcct gcctgcgata tgcgaacgca gcgaaccgtt gtccaagatg atgccttggg
     1441 tttggttgcg gtaatagagc ttgtcgccgt tgatgctgcc gcccaagtgc gggtcggcga
     1501 tgctgccgcc gagggttacg gcggcattca ggctgccttt gacggtttgt ccgacgggca
     1561 ggaagttgcg gaatacttcg gcattggcaa cggtcaggtt gagtttcccg cccaggggcg
     1621 aggtactgaa agaattactt tgcccgacgg tgatgttgcc gttgattttc ccgtagttgc
     1681 tgctgccgtt gacggcggca ttgacggacg gagagccgac ccgtccgccg atttgcgcgg
     1741 cggcattcag gctgccggta atgttttgcg cggcggcggg cagaaagggc ttcaatgcgc
     1801 ccaagtcggg aagggaggcg gtaatcctgc cgccgagcgg tgcatttgcc atattgccgc
     1861 cgaaggcgtt gccgatgtcc aaatcggcgt taatccgccc gaaacgcgcg ccgccgtcaa
     1921 gcaggattcc gatacggtcg ttttgaaagc gcgttttcag ggaaaatgcg ttcaaaccca
     1981 aagcctgccc gccgggcaat acggcatcgc cgctttgccg gctgatattg aggtagccgc
     2041 gcgcgttgcg cccgtaggcg acatcccagt cgccgtttaa aaccagattg tgttcgaagg
     2101 gcggtttgaa gaaattgtgc aactcggcga tatgcagacc gtgtgcgccg ccttttgccg
     2161 atatgccggt ttttttatcc caagaaaagt gttgcaggtt gaggctgccg cccattgcct
     2221 gccaatttgc cgcacttgcc gccacgcgtt ccgcaccggc ttcgagcgtc atacggtttt
     2281 gcagcttgag gttgaatgcg ccgccgatgt cgaggatgcc gatgctgcct ttccatcggg
     2341 taagttccct gttgatgccg cctgaagcgt ccaaatcgaa tttgaacggt ttgccatcca
     2401 gcgtcatggc ggcgtgtgtg cggatgcggt gctgcacgcc cgtgccgtcc agcatcaggt
     2461 cggcggtatc gacaaccgcc gctccgcccg acagcgaaag gcggctgcct ttgatgtcgg
     2521 cgcgtatcgg gcggcttgtg tcgggcgaac ctttgagcgt gaaatcgagc gaacggatgt
     2581 ctgccgcctt gccgatgtgc aggttgcgcg ccgcgccgga aaggtcggtt tcaaaggttc
     2641 ggatgccgcc gtccaaatcg ccggaaaggt gtccgcgtac atttaaagac cccgcgagtc
     2701 cgaaaccgaa acgggataaa tcgggtgcgg tgatgttgag gttgagccgg tcgccttttt
     2761 tgccgaagcc gccgtctgtt ttaataatgt tccgccccag ccgcaaatcg acggcggcac
     2821 gcggaaggtg gcgggactcg taaacaatgt cggcactgcc ggcaatcggt acgccgttga
     2881 acgtgccggg taaaaaccgc attttgcctg tgaatttctc ttttgccagt tcgccggcaa
     2941 ggtttattga gccgttgata ttgcctgccg gaagttgcgg attgatgcgc gaagggtcga
     3001 atgcgcggga acggatgtcc agcttgagca ggcggtcttt aaacagctcg agatagcctt
     3061 gcgcggtcag gctgccttgc ccggcggcga tgttgacggt gtcgagcacc agtttccgct
     3121 gtccgtttgc ggggtcgctt gcaatggcga ggctgccgtc cgtgcgcgcc gtgccgatgc
     3181 cgagttgcca agagattttg ggcgaggcgg tcgtgccacc gatgccgatg ctgccgtcca
     3241 acctgccttt gaacgcggtt tgcagtacgt cttccgcgcc gacggagttg atgcctatat
     3301 ttaaatcgag gatgtctttt tcggtgtcga ttttgcccga cagcctgatg ccgccccgtc
     3361 cgagcagggc ggcggacgta ttgccgatat gcaccgtgcc gtcctgccgg atgacaaagc
     3421 cgcctaaaac ctgacggacg gggatgccgt tgcggtcggc aaagccggct ttggtgtttt
     3481 ccaaatcgag cgaaccttcc agcgcgatgc cgtctgaaaa cgacgggatg gcggtcaggt
     3541 cgaaattcag cccggcatcg ggcagggagg gcacgaaggc ggccgggttg atgttgaacc
     3601 ctttgaccag cacttcttcc aatgttttat ccaatgattc ggcaaacggg tggatgacgg
     3661 attttcccga gaggcggata ttgccgccgt cgatcgccag ttcggcgcgc acatccttca
     3721 ggctgccgct cagccgcgcc gtactgtgta tggttttgcc ttcgagtccg cctttggtgt
     3781 aaatggcggt atcgagggca aacggttttt tcaagccgac cgaggctgac cccgacgaac
     3841 tgctccacgg cgtgtcggcg gccttcaggt cgaggcggtg tcctttgcgg tcgtaacggt
     3901 atgaagcatc cagccgttcg agatagacgg tttgtttgtc aaaggctttg cccatgctga
     3961 ttttgcccgt ctcgaagcgg tcgagataga cggcggcagg caggtctatg ctgtcgggaa
     4021 ggctgagcgg agggcgttct tctttaggcg gagtcggttt ggtaacgatg gcgatgtcgc
     4081 cggcggaaat ttcggtaatg tgcaggctgc ggcgcatcag ttcggacggt ttccacgcga
     4141 agcggaagcg gctgatttta aggtctgccc cctcggtttc tatcgaccag ttgtcgccgt
     4201 cgaagccgtc gagcagcgtg cctttgaggt tttgggagga aatgtttacg ccgaaccaag
     4261 acgggatttg gtacagcccg aagcgcaaac ccgcttccgt actggcgagc cagccgagga
     4321 aacatactgc caaaatcagg acagacagca gtgccgccga cagcttcagc agcgggcggc
     4381 gtttttttgc cggcggggca gggcggtgtt cagacggcat tttgcgcgtg ccgttttcgg
     4441 tcggatcggt atctgtcggt gcggtatcgg tcataatgtc tgtttcaaaa tggtttgctg
     4501 caatgccgtc tgaagcggcc gtatcggttt agaagcgtgt tcccaagctg atgtgccagc
     4561 ggattttctt atcgctgtgc ccgtaggcga tgtcgaagga aaacggcgca agcgggctga
     4621 accagcgcac gcccagtccc gaaccgtgtt tcagcttcat acgtttgaaa ttggcggcgg
     4681 catcgcccat atcgtggaat accgcgccgg aaagggtgcg cgtaaacggc agttggtatt
     4741 ccaggctgcc caccaagagg gcgcgttcgg gcaggaccga tccgttcggg ccggcaagtc
     4801 cgatgctgtc gagttcgtaa ccgcgcacgg aagacgcgcc accgctgcgg aacatcagcc
     4861 ccgaggggac atcggcattg tcgcgggcaa gggtgtaacc cgcttgtccg cgtatgatga
     4921 acgtgccgag tttcttgttt tcgggcgtga agaaataacc tgcgcgggca gaggtgcgga
     4981 tcagcgcggt ggaggacagg aatgtgccca aagtcgtacc gattttgccg tcgaggtaat
     5041 ggccgttttc gggatgcagc acgttgttga gcagctggcg tttccaagag gcggtcagca
     5101 tcgtggcgtg gctgttgccc aaatcgatat ccgagccggg gattttccgg ccttctgcga
     5161 gaaactctgc ccccagcctg gcatcgatgc ccgcgcggtc gcgcacatac cagatgccgc
     5221 cggagaaggc gcgtttttcg aggttttggg tggtcgaacg gttgtaggaa acgttgcttg
     5281 tccagtagtt gccccgatag ttgcgcggct ggctgatgcc ggcggcaagc gtggtttcgt
     5341 atttgtccat atcccagacg accgagccga tatagccttt gttgaagagg ttgtaatagt
     5401 cgtaggcgat tttgccgccc aaaccgtatt ccgaatcgag gcggatgccg gtttcgagct
     5461 tgtggcgttt gacctcggtt acgctgactt tgacggggac gcggtcgcct tggagacggt
     5521 cgaagtcggc ttgtacggac gcgccggaat aatgcccgtt ttgttcgagt gcctgttgga
     5581 agtcgagcag caggtcgagg tcgtagggcg tgcccggttg gaagcgcgcc aggccggaga
     5641 cgatttgttc ggggtaacgc tgtgtgccgg tgatttcaaa gtcgccgaag gcgatggggc
     5701 ggccgctgtc cacgacgacg ttcaaatcga cggtggcggt atcggggttg acggccgccc
     5761 gggtgttgcc gagcttggca agcgggtagc ctttgcgcgt taccgcgccg aggacggaag
     5821 ttttgctgtt ttcccaactg tcctgatcga aatcgctgcc taccggctgc tgccagtttt
     5881 ccagcgcgtt gcggtagtat tcggcgaggt tgccgtctga aaggatgtcg ccgaggatgg
     5941 cgacgccgac gttggcgatt ttggtgcgcg ggcccggtgt gatgtgcacc gtataagctc
     6001 cgtctttttc cgtcagactg actttgctgc tgaaatagcc tttgctgcgg agcatcgttt
     6061 taacgttgtc cggtgcttct tcggcgagga agcctgtctg ttccttatcc aaaacctctt
     6121 cctgctgctg cgtgataagc ggcaggtgtt cttcgaccat atctttgatt tcactgtcct
     6181 gcgtgtcgat gcggacgggg aatttgggtt ttaatttaac tgattcggtg tcagggcttt
     6241 tgtttttgaa caatgcgaaa cccgccgcct tgttttcgga aaggtcggcg gcaggcgcgt
     6301 atgcgtgcgg aaagaaaaat aaagccggca ggagcagggc ggtcggtttg atcatcatgg
     6361 tacgggtgtc gtgcatcggt gtggcggatt tcgattctaa cactgttcgg acggggcggg
     6421 acgcttcaga cggcatactg ccgccggtat ttcggaggtt gcggcccgcc ggaaatgtat
     6481 ctgcttgttt tagggtattt tgtttctaat ccgtatatgg aaaacaggct gcgtttcgga
     6541 atagtgccgg aaaataggca ggattaaata aatttatttt cgttcaataa attaaaccgt
     6601 tgcgccgaat gataaagcaa agcctgtccg gcactgcata gggcaaatca atgcaacctt
     6661 aaaacacagc cgaaaaataa aaaaaacaca gagaatgagt tttcttttaa aatacaacat
     6721 tttttaacat atcacaacat aaggaagaat tatggcgttc ggcaaatcct tatttcatgc
     6781 gatcggccgc gtcagcctgg tcaggcagat tgccgccggt ttggcgttgg gtatcgtaat
     6841 cggttcggtt tccccgcaac tgggcttggc ggcaggcttg ttcggcagcc tgtttgtcgg
     6901 cgcgctcaaa gcggtcgcgc cggttttggt atttattttg gtggcggcca caatcgcgca
     6961 gcaccaaaaa ggcaacaagg cgcatatcag gccgattatc gtcctttacc tcatcggtac
     7021 gttttccgca gccctgaccg ccgtcatcgc tggtatggtt ttcccgacgc acattgtttt
     7081 ggcgggcgcg ggcgatgtgt ccgccgcgcc gccttccggc attgtggaag tattgaaatc
     7141 gctgctgatg aacctggtcg ccaacccgat taacgcgatt gccaatgcca actatatcgg
     7201 cattttggct tgggctttgg ttttgggcgc ggcgttgcgg aatcacggtt cggacgttac
     7261 gcggcaggtc gttgccgatt tggcggaagc ggtttccacc gtcgtgaaat ggattatccg
     7321 ttttgcccct ttgggcattt tcgggctggt gtcgtccaca atcgcggaga cgggtttcgg
     7381 cgcgctggcg ggttatgcga agctgcttgc ggtactgttg ggctgtatgg cgtttatcgc
     7441 gctggtggtc aatcccgcca tcgtgtggtg gaaaatccgc cgcaaccctt atccgctggt
     7501 gtttacctgc ctgcgcgaaa gcggcgtgta tgccttcttt acccgttctt ccgccgccaa
     7561 tatccccgtg aatatggctt tggcgaaaaa actggggctg cacgaagaca cttattcgat
     7621 ttccatcccg ttgggggcga ccgtcaatat gggcggcgcg gcgattacga ttaccgtttt
     7681 ggctatggcg gcggcgtaca cccaaggcat acaggttgat tttgccaccg cgctgctgtt
     7741 gagcctggtg gcgacggtca gcgcgtgcgg cgcgtccggt gtggcgggcg gttcgctgct
     7801 gctgattccg ctggcgtgca gcctgttcgg catcagcaac gatgtcgcta tgcaggtggt
     7861 cgccgtcggc tttatcatcg gcgtgattca ggattcggcg gaaacggcgt tgaattcttc
     7921 aaccgatgtt ttgtttaccg ccgccgccga tttgggccga cagagaaacc gggcggaata
     7981 aaccaagcta tgccgtctga agcccttttg gcgttcagac ggcatatttt tacaatttta
     8041 tgggcatatg gcggaaacaa taccggctgc cttgaatatg aaggagatga tgatgtttaa
     8101 acgcagtgtg attgcaatgg cttgtattgt tgccctttca gcctgtgggg gcggcggtgg
     8161 cggatcgccc gatgttaagt cggcggacac gctgtcaaaa cctgccgccc ctgttgttac
     8221 tgaagatgtc ggggaagagg tgctgccgaa agaaaagaaa gatgaggagg cggtgagtgg
     8281 tgcgccgcaa gccgatacgc aggacgcaac cgccggaaaa ggcggtcaag atatggcggc
     8341 agtttcggca gaaaatacag gcaatggcgg tgcggcaaca acggataatc ccgaaaataa
     8401 agacgaggga ccgcaaaatg atatgccgca aaatgccgcc gatacagata gttcgacacc
     8461 gaatcacacc cctgcaccga atatgccaac cagagatatg ggaaaccaag caccggatgc
     8521 cggggaatcg gcacaaccgg caaaccaacc ggatatggca aatgcggcgg acggaatgca
     8581 gggggacgat ccgtcggcag gggaaaatgc cggcaatacg gcagatcaag ctgcaaatca
     8641 agctgaaaac aatcaagtcg gcggctctca aaatcctgcc tcttcaacca atcctaacgc
     8701 cacgaatggc ggcagcgatt ttggaaggat aaatgtagct aatggcatca agcttgacag
     8761 cggttcggaa aatgtaacgt tgacacattg taaagacaaa gtatgcgata gagatttctt
     8821 agatgaagaa gcaccaccaa aatcagaatt tgaaaaatta agtgatgaag aaaaaattaa
     8881 taaatataaa aaagacgagc aacgagagaa ttttgtcggt ttggttgctg acagggtaga
     8941 aaagaatgga actaacaaat atgtcatcat ttataaagac aagtccgctt catcttcatc
     9001 tgcgcgattc aggcgttctg cacggtcgag gcggtcgctt ccggccgaga tgccgctgat
     9061 tcccgtcaat caggcggata cgctgattgt cgatggggaa gcggtcagcc tgacggggca
     9121 ttccggcaat atcttcgcgc cagaagggaa ttaccggtat ctgacttacg gggcggaaaa
     9181 attgcccggc ggatcgtatg ccctccgtgt gcaaggcgaa caggcaaaag gcgaaatgct
     9241 tgctggcacg gccgtgtaca acggcgaagt gctgcatttc catacggaaa acggccgtcc
     9301 gtccccgttc agaggcaggt ttgccgcaaa agtcgatttc ggcagcaaat ctgtggacgg
     9361 cattatcgac agcggcgatg atttgcatat gggtaagcaa aaattcaaag ccgccatcga
     9421 tggaaacggc tttaagggga attggacgga aaatggcggc ggggatgttt ccggaaggtt
     9481 ttacggcccg gccggcgagg aagtggcggg aaaatacagc tatcgcccga cagatgcgga
     9541 aaagggcgga ttcggcgtgt ttgccggcaa aaaagagcag gattgatgct gatgccgcct
     9601 gaagcggggg cgttcagacg gcattgtgat gtttggggtg gggtccggtt caagtccgca
     9661 tcagttgata aacccgtcgt ctttcatttt cttttcggaa aaatactggc cgaataccag
     9721 cagggcagcg aggatgatga caaacagtcc cgccatagtc agagtgattt tgaaaaacaa
     9781 ttccgcttcg agaaaagaaa accagagttg cacaatcagc agcagcaccc agccggcaaa
     9841 cagtccgaga caggcataaa cgccgagctt aaacacggtc ccgctccaaa atgacgacgt
     9901 aggcctcgcg tttgaaaaac agcagcatac ggcgcaaatc tttttccatc gttacga
//
LOCUS       DAXHMX010000076         9793 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHMX010000076 DAXHMX010000000
VERSION     DAXHMX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.076"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(9..1304)
                     /locus_tag="ACFM4S_001308"
     CDS             complement(9..1304)
                     /locus_tag="ACFM4S_001308"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6050975.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLERAQWFW
                     QQSLQAVELVDERVRKHAVDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLSPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDHEFIDDPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1430..2317)
                     /locus_tag="ACFM4S_001309"
     CDS             complement(1430..2317)
                     /locus_tag="ACFM4S_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6050976.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGACPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAH"
     gene            complement(2317..3282)
                     /locus_tag="ACFM4S_001310"
     CDS             complement(2317..3282)
                     /locus_tag="ACFM4S_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6050977.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3298..4422)
                     /gene="potA"
                     /locus_tag="ACFM4S_001311"
     CDS             complement(3298..4422)
                     /gene="potA"
                     /locus_tag="ACFM4S_001311"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6050978.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4617..4724)
                     /locus_tag="ACFM4S_001312"
                     /pseudo
     CDS             complement(4617..4724)
                     /locus_tag="ACFM4S_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="ACFM4S_001313"
     CDS             complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="ACFM4S_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6050979.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5295..6230)
                     /gene="secF"
                     /locus_tag="ACFM4S_001314"
     CDS             complement(5295..6230)
                     /gene="secF"
                     /locus_tag="ACFM4S_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6050980.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6234..8087)
                     /gene="secD"
                     /locus_tag="ACFM4S_001315"
     CDS             complement(6234..8087)
                     /gene="secD"
                     /locus_tag="ACFM4S_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6050981.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="ACFM4S_001316"
     CDS             complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="ACFM4S_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6050982.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8693..>9793
                     /locus_tag="ACFM4S_001317"
     CDS             8693..>9793
                     /locus_tag="ACFM4S_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6050983.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCQPQKGKIFRL
                     DDDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2508 a         2602 c         2434 g         2249 t
ORIGIN      
        1 tgcgacgctt aacggctgtc tttccactga taatatttcg agccgaggaa agaaccgagt
       61 ttgcgcaaaa acggttgcag gataatattc ggctggcgca accgctcaaa cggcttcaga
      121 cggcattcgt cccctaaaat tgcttcggca accgccagac ctgcaatgcc tgttatcgcc
      181 atcccgtgtc cggaataacc ttgcgcataa aaaacattcg cggccaaacg tccgaaatgc
      241 gggacaaggt tggcggtaat gtcgcactcc ccgccccacg aatattcgat tctaacatcg
      301 gcaagctgcg gaaaaacttt aagcatatct tggcggacaa gttcggtcat acgcgcggga
      361 tcgtcgataa attcatggtc tttaccgccg aaaagcaatc tgccgtccgc actgaggcgg
      421 taatagtcca aaatatggcg gttgtcgcat actgccatat tgttgcggat aagccctttt
      481 gcgcgcgccc ccaagggttc ggtggcaata ataaaggtgc tgacggcaat cgccttgcgt
      541 tccaaaggct taaatatcgg actcaaaccg gcataggtat tgacggcgta caccagattt
      601 ttgcattcga cgctgccttc gggcgtgtaa accagccaac cgttttgatg cggttcgatg
      661 cgcgtcatcg gggattgctc gaaaatctgc gcaccggctt cggcagcggc acgagcgatg
      721 cccaaagtgt aattgagcgg atgcaggtgt ccggataagg ggtcgaattg tgccccttgg
      781 tacatatcgc tgtcaagctg ctgtttcaac tcggctttat cccaaagttg ataatgactc
      841 gcaccgtaat gccgttgggc gtgttcatgc cactgctgca actcttccca atgctgcgga
      901 cggacggcaa ccgtggcata accgcgctgc caatcacaat cgacggcatg tttgcggacg
      961 cgttcgtcca ccagttcgac cgcctgcaaa gactgttgcc aaaaccattg cgcccgctcc
     1021 aaaccaacct gtttttcaat ttcctccata ccgcaggcgt agtcgctgat aacctgaccg
     1081 ccgctcctgc cggacgcgcc gaagccgata cgcgcggctt ccaacacaac cgtttcatgt
     1141 ccctgctccg ccaagggcaa tgcagtgcac aaaccactca atccgccgcc gatgatacag
     1201 gtatcggttt tcagacggca ttgaagtttc ggataaacag tatgaggatt aaccgaacta
     1261 aaataataag aaggcagata ttcttgaaaa tcaggacgaa tcattatgtt tactttatca
     1321 ggtgtatttt cagacggcat gatatggttt gccgtaccat gccgtctgaa cgggaaatta
     1381 ctttaagaaa acaggctgac ctgataatgc ggtcagcctg ccctgtcggt caatgcgctt
     1441 tctcagcagc caatttttct tggcggtagg cttctgccgc ttctcggtcg cgcttggttg
     1501 cctgcctcat catccaataa ttgacgatga tgaccaatgt tccgatgatg ccgattagga
     1561 tggtcgccaa gacattcatc tgaggatcaa gacccagctt gattttggag aaaatcacct
     1621 gcggcaatgt ggatgaaccg gggccggaga ggaatgaggt aattaccaaa tcatccaaag
     1681 acagggtaat gccgagcaga aagcctgaag cgatggcagg ggcaatcaaa ggcaaagtga
     1741 tgacaaaaaa gattttcagc ggacacgcgc ccaaatccat tgcggcttct tcgagcgatt
     1801 ggtcaagctc aaccagacgc gaacggataa caacggtaat gtacgccata cacagcgtcg
     1861 tatgtccgag gaagatggtg aaaaagccac gatcgaagta gagatgttgt aaccattcgc
     1921 tgccctgcaa aaatatctgt acctgaataa tcagcagcag catagacaga ccggtaatca
     1981 cgtcgggcat caccataggt gcggaaatca tgccagcgaa caaggtactg ccgcgaaaac
     2041 gtttaatccg cgccatcgca tagcctgcca gcgtgcccaa aacgacggcg gcaagcgaag
     2101 acacaacggc aatccgcagc gacagccaag cggcttccaa gatggtgtcg ttttccagca
     2161 atgcgccgta ccacttggtc gaaaagccgc cccaaacggt taccagcttg gattcgttaa
     2221 acgaatagat gaccaaaaca accagcggga tatacagaaa cgccagcgac agtgccaaca
     2281 tcagtttcaa gaaccaagat aatttggatt tctgcattat ttggctcctt cttccaattc
     2341 gcggttttca taatgctgaa acagggcaat cggcacgacc agcagcgcga ccatcacgac
     2401 ggcgacggcg gaagccagcg gccagttgtt ttgatcgaag aacgcctgcc acaagacttt
     2461 accaatcatc aggttttccg aaccgccgac cagctcggga atgacgaact cgccgacagc
     2521 agggacgaaa accagcatgg agcctgcaat aatgccggtt ttcgacaaag gcagggtaat
     2581 cgtcaagaac gatttgaccg gccccgcgcc caaatcggaa gccgcttcaa gcaggcggtt
     2641 gtcgagtttc accagttgcg tgtatagcgg cagaatcata aacggcagat aggcgtaaac
     2701 catcaccaaa ttgagcgaaa aggcattgta gaacaaatcc aaaggctcgc tgataatacc
     2761 cattttaatc aacaggttgt ttacaatgcc gttatgcccg agcagaccca tccacgcata
     2821 gacgcgcaac aggaacgatg tccaaaaggg cagcataatg gcaagcagca aaccattgcg
     2881 gacagaagga ttggcacgag aaatcgcata ggcggtcgga taaccgacca acagacaaat
     2941 taccgtcgta gtcagcgcag tcttaattga agaccaataa gtcatcagat agatattgct
     3001 gttttcaccg tcgccgaacg gattgagcgt actccaaaaa ttttggaaga tgtctgcata
     3061 attttggtag ctgacagcaa tattcagacg acccaaatcc tcatctatcg tcgttaaagg
     3121 agtaaacggc gggatggcga tttcttgttc ggcaaagctg attttcagca cgatggcgaa
     3181 cggaatcaga aacagcacca aaagccaaat atacggtacg gcaatcaccg cacgctgccc
     3241 cggacggcgg aacagtttgt ttttcagttt attaaggttc attgcattcc ccttaaatca
     3301 acggaacaac ggagtcggtt ggttttccgg ccagctgata tagacggttt cgtcccaagt
     3361 cggcggtgta atgttgcgca cataccagta aggggcgggg acttggcttt tgacgacgcg
     3421 cccgttgccg agcttgatat ggtaaatggc gaagctgccc aaataggcga tttcttttac
     3481 cgtgcctttc gcccagttgt agtcgcccaa atattcgggt ttttctttat ataaatcaat
     3541 atcctctggt cgaatactaa cccaaaggtc ctgctcgctc ggaccaccca aaccgtgatc
     3601 gatgcggacg tggttttcca aaccttcgca ttcgataacg gcatagtcgg catgatcttc
     3661 aatcaccaca ccgtcaaaga tgttggtttc gccgataaac tcggcagtga agcggctgtt
     3721 gggatagtcg tacacgtcgc tgggtgtgcc gacttgctgc aactgaccgt cagacataat
     3781 ggcgatgcgg gtcgccatcg tcatcgcctc ttcttggtcg tgcgtaacca taatacaggt
     3841 tacgccgact tgttccagcg tattgaccaa ctcaagctgg gtttgttggc gcagtttttt
     3901 gtccaatgca ccgaggggct catccagcag tagaattttc ggacgttttg ccagactgcg
     3961 tgccaaagca atgcgctgct gctgaccgcc ggacaattgg tgcggtttgc gtttagcaaa
     4021 tttggtcatc tgaaccaggc ggagcatttc ttcgacgcgc gcggcgattt cgcctttagg
     4081 cattttgtcc tgtttcagac cgaaggcaat gttttgttct acggtcatat gcggaaaaag
     4141 cgcgtaactt tggaacatca tattgatggg gcgatcatag ggtgcaagtt tggtaatatc
     4201 ctgaccatca aggataattt ttccctgatt gggactttcc atacccgcca gcatacgcag
     4261 cagtgtagat tttccgctgc cggaactgcc caaaagggcg aagatttcgt gttgataaat
     4321 gtccaagtcg atgttatcga cagcgtaatt gtcaccaaac tttttcacca aaccttggat
     4381 tttgagataa ggtttggctg aagacgcagt ggttgcggtc ataatggcaa tactccaata
     4441 aaaagacgag taccggcaaa acggattttc gaatgggtga taaaaagctg tttgattgct
     4501 ggcgggagtt aaacgtttga tgccgtctga aactcttgta aagcgcacgg gcagcatgaa
     4561 atggaacaag attccaaaga actttatatt atattagttt atgcggtttt cgggcaatat
     4621 agtggattaa atttaaacca gtacagcgtt gccttgcctt gccgtactat ttgtactgtc
     4681 tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac tataaatatg gatttgagct
     4741 tgtcggaaag caacagaaaa gaaaacaccg cccatttttc tgggcggtgt cggaaagcgt
     4801 aattatttac gcagacccaa gcgggtaatc aacgcgcgat acgtatcggg ctgggtacgg
     4861 cgcaagtagg ccagcaggcg gcggcgttgg ctgaccattt tcaacaggcc gcgacggctg
     4921 tggtggtctt tggggttggc tttgaagtgg ggggtcaggt cgttgatgcg gaaagtcaac
     4981 agagcgactt gtacttcgga agagccggtg tcgccttctt tgcgttggaa atctttaacg
     5041 atttgtgctt tttgttctac ggtcagtgcc ataatgaaaa ctccaaaaat ataagaatcc
     5101 ccatagggat tcagacaagt ttgccaagcc tgaagacaac ggcaaactcc ctatgcccaa
     5161 gataggacaa cgtggcatta tgacacaatt cccgcatttc tgcacaatat tttaagaccg
     5221 tggaagtaac cgttaaaaat gccgtctgaa gcattgtctg cttgagacgg caatgttcag
     5281 acggcatatg cgcttcaaac cacgatttct tctttcttct tcggttcttt accgatattg
     5341 tcgcggctca aaccgaacat tagcagaagc gggctggcaa ccaatacgga agaataaatg
     5401 ccgaacacga tgccaatggt caacgccata gaaaagccgt gcaaggccgc accgccgaac
     5461 accagcatgg atacgaccat cgcctcggtc gaaccgtggg taatgatggt gcggctcatc
     5521 gttgcggtaa tcgcgttgtc gatgacttcc ggcacggcat gtccgcgcat cgccggcttg
     5581 cggaagtttt cacggatacg gtcgaagacg acgacggatt cgttcacaga atagcccaat
     5641 acggcaagga tacccgccaa gacggtcagc gaaaattccc attggaagaa ggcaaagcag
     5701 ccgagaataa tcacgatgtc gtgcatattg gcgataatgg cagatacggc aaaacgccat
     5761 tcaaaacgca tcgacaggta aataatgatg ccgataacga caaaacctaa agccatcaat
     5821 ccattactta ccaattcctc accgacttgc gggccgataa attcgacttg gcgcaaggta
     5881 acgtcgggac tgtctttttt cagcaaatcc ataacctgat tggacaactg tgcggaagta
     5941 acaccttctt tgttcggcag gcggatcatg atgtgtttgt tcgtacccaa tgcctgaacc
     6001 tgtacatcac ctattttcag cgtatcgagg cgttcgcgca tcttattgac atccgcaccc
     6061 tgctgatatt ggacttccat taccgtaccg ccggtaaatt cgacagagaa attcagacct
     6121 ctggtaacca aaaagaacac ggcagcgata aacgtaacca acgaaatgaa ggtcgtcagt
     6181 ttgccgtagc tcataaacgg aatatcgcgt ttgattttaa agagttccat agcttactcc
     6241 ttacctgccg cagtttcggc ttcaggtttc cataccgcac cgatggaaat attctgcaat
     6301 ttgcgtctgc gtccgtacca cagattgacc aacgcacgga atacgacgac ggaggaatac
     6361 atcgaagtca gaatacccaa acagtgtacg accgcaaaac cgcgtaccgg gccggaaccg
     6421 aataccaaaa gcgcgatacc ggcaatcagc gaagtcaggt tggaatcgac aatggtcgcc
     6481 catgcgtgtt ggaaaccgag attgattgcc tgctgcggcg gcacgccggc acgcaattct
     6541 tcgcggatac gttcgttaat caagacgttg gagtcgattg ccatacccaa agtcaacgcc
     6601 agcgcggcca tacccggtaa cgtcaacgtt gcctgcatgg cagacaaaat accgattagg
     6661 aacagtatgt tggcactcaa tgcaatggta gaaaagaaac ccatcagacg atagtaaacc
     6721 accatgaatg cagcaacgat ggcaaaaccc cataaagtcg aatggaagcc tttttcgatg
     6781 ttctccttac ccaaagacgg accgatggta cgttcttcga caatctgcat cggcgcggca
     6841 agagaaccgg cacgcaacag caaagacgta tcattggctt cggctgtcgt catgcttccg
     6901 gaaatttcca cgcgtccgcc ggtaatggca gtacggataa ccggcgcggt tacaacctcg
     6961 gattttcctt ggtcgatcaa aaccatcgcc atgcgtttgc cgacatttgc ggcagtcagt
     7021 tcgccaaaaa tgctgccacc cgcgctgtcc aagctcagac tgacggcagg tgcgcccatt
     7081 tggtcgaaac tcggttgcgc atcgttgatg ttgtcgcccg tcagctcgac ctgtttgctg
     7141 attagcagag tttcgggacg atctccgccg cttgaaagca gctcataacc gctcggcacg
     7201 ttgccttcca atgcctcgcg caacttggca ggatcgtcct ccaccatacg caattccaaa
     7261 gtcgcggtac ggccgatgat gtcttttgcc ttggcagtat cctgaacgcc cggaagctgc
     7321 acgacgatac ggtcggcacc ggactgctgg atgacgggtt cggccacgcc caactcgttc
     7381 acacggttgt gcagggtagt gatgttctgt ttgaccgcat cggaacgcac tttattgacc
     7441 gcctcttccg aaagcgtcaa gacgatattg ctgccgtctg aattcagcgt tgcttcagga
     7501 aacagcttgc gcaactgcgg cagagccttt tgcacatcac ctgcatcctg caaagggacg
     7561 gtcaggctgt ttccagcctg acgcaccgtg ccgctgcgga ttttttcgcg gcgcagttcg
     7621 cggcggatgt cgcccgaata acgttcaaac gttttctgca tcgccgcttt catatcgacc
     7681 tgcatggtga aatgcacgcc gccgcgcaag tccaaaccca aaaacatcgg attggctttg
     7741 attttcgcca tccattcggg gctgtccgcc aacaggttga gcgcggtaat atacccttcg
     7801 cccaaagtgt tttcgatgac gtcgcgcgct ttaagctgcg tttcggtgtc tttgaaacgc
     7861 actttcagtg aattgtccac aacaaacatc ccgtcggtct gaatacccgc gttttgcagc
     7921 gcggcatcca ctttgaattg agtctgttcg ttgatgatga tggcttgtcg gttggtcgat
     7981 acctgcacgg cgggtgtttc gccgaatagg ttgggcagcg aatacactgc ggcaaccgca
     8041 atcgtgaaca caatcagcag atatttccat aaaggataac ggttcatcat tgttccttaa
     8101 tggttggaac cccacccttt cggtggtgtc ggaatcgggc tatttcagaa gaggcaaaaa
     8161 cccttcccag ccaggcaaga ccggaaggcg gcatcttgaa tatgccgccc tgcgtgtcgg
     8221 aacatggtca agccttcggt tggaattcaa aacaaagtgc cgcattcggg cttttccgat
     8281 gcggcttgtc ggcacaaatc aatcgacttt tgcagaaatc gcattgcgtt ccacttcgac
     8341 ctcgattttt gtaccctgtc cgatatccac ggtaaaaaac tgttcgccga ctctggttac
     8401 cttacccttg aaacctgccg ccaagaccac tttgtcgccg actttcaagg cggcaagcat
     8461 tgcctgatgc gctttgaatt tcttttgctg cggacgcatg atcaggaagt agaacaccac
     8521 cataatcaac actaaaggag caaattgtgc aacagcttga ttcataattt atccgttctt
     8581 tctaatatgg ttgaaaatcg agaggggtat ataataacat aagaccgtaa acaatatatc
     8641 gggtttgccg tccgtaccga ccgtataccg cagcctgccc gtccacaaac ccatgtcctt
     8701 gacccgccta atcttgaaat tctatgcact gttgcgcctt tttttgggca aaaacgcccg
     8761 caccgcatgg atttcgcatc ccgcctgtgc cgggcacgaa cccggcgcaa accatcccga
     8821 ttcgcccgac cgcatcctct gcatcgagca ggcattgcgc cgcgccggta tttggcagca
     8881 cctccaaacc atagaggcgg aagaaatcag cgatacgcgc ctcgcacttg tccactcgag
     8941 caaatatctg aaccgtttgg aatcttgcca gccccaaaaa ggcaagattt tccgcctgga
     9001 tgacgacact gcaatcagca caggctcgct gtctgccgca cgctttgccg ccggttcggc
     9061 agttcaggca gtcgacatgg tcatgaaccg caaagcatgg catgcctttt gcgccgcccg
     9121 cccgcccgga caccatgcgg gcagcggcaa agccggcgga ttctgcctgc tgaacaacgt
     9181 tgccgccggc gtcatgcatg ccattgccga ataccgcctg aaacgcattg ccgtcatcga
     9241 tttcgatgtc cactacggcg acggtacggc agaaatattc aaagacgatc cgcgcatcct
     9301 gtttttcaac ctgtttgaaa ccgacctttt ccccttcccc gaaaacaacg atatgcccga
     9361 cggcggcaat atggtgcacc tgcccttgcc gccgggaacg ggcagccgca cattccgcga
     9421 agccgtccgc aggcagtggc tgccccgact tgccgcattc aaacccgaac tggtgctgct
     9481 atcggcagga ttcgacgcac accgtctaga cgaatcgggc aggctcaacc tgcacgaggc
     9541 ggattttgcc tggctgacac acaaaatcat tcagacggca tcgggctgcc ccggcaaaat
     9601 cgtatccgta ctcgaaggcg gctacaccct tgaacctctg gcacaatctg ctgccgagca
     9661 tatccgcgtg ctggcagggc tgggcaaatc cgatgccgca accgcctatc agaaaacact
     9721 ggacccaaca aaaaaacggt ttgccaaacc gaaaaccgga caggtgcgcc aacctaccca
     9781 atccgaccgt tac
//
LOCUS       DAXHMX010000077         9505 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHMX010000077 DAXHMX010000000
VERSION     DAXHMX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.077"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1067..1735)
                     /locus_tag="ACFM4S_001318"
     CDS             complement(1067..1735)
                     /locus_tag="ACFM4S_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6050984.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1732..2400)
                     /gene="hda"
                     /locus_tag="ACFM4S_001319"
     CDS             complement(1732..2400)
                     /gene="hda"
                     /locus_tag="ACFM4S_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6050985.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2505..3095)
                     /locus_tag="ACFM4S_001320"
                     /pseudo
     CDS             complement(2505..3095)
                     /locus_tag="ACFM4S_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3305..4022)
                     /locus_tag="ACFM4S_001321"
                     /pseudo
     CDS             complement(3305..4022)
                     /locus_tag="ACFM4S_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
     gene            4244..4447
                     /locus_tag="ACFM4S_001322"
     CDS             4244..4447
                     /locus_tag="ACFM4S_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC6050986.1"
                     /translation="MKKKQESMTDWHRADIVARLKKAGWSVRALSIEANLAPNTLGKA
                     LDAPYLKGERIIAAAIGVPAEEI"
     gene            4569..6557
                     /locus_tag="ACFM4S_001323"
     CDS             4569..6557
                     /locus_tag="ACFM4S_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6050987.1"
                     /translation="MKISASDIAKLGIPSLPTDRQGIEYHAKKNNWQHCFEQKGRGRP
                     KKLYEIASLPAEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQ
                     RSVADARCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRS
                     AKVGERTLDGWISAYLKAENAIERLVALAPKTTKAVKPIESYGWLPMFMRFHNIPSAP
                     KLAHSYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYV
                     KRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAES
                     CVAVSDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQ
                     GRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQ
                     KYLKKLPSWSRFIADVVKCIDDYNNRPHGELPRHPDGGHYSPKAYREIRLEQDGIAPD
                     MLSAEELATMFMPQEVRKVQRGWLDLFSNSYFSTELAEYHKDEVRVSYDLSDASAVNV
                     FDMDGKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQ
                     AAAWDELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADM"
     gene            complement(6624..6881)
                     /locus_tag="ACFM4S_001324"
     CDS             complement(6624..6881)
                     /locus_tag="ACFM4S_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6050988.1"
                     /translation="MKEFPDLEKRLIMLEVAVQDLEERSLTDSFVLAWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLEIVESAQGLA"
     gene            6927..7685
                     /locus_tag="ACFM4S_001325"
                     /pseudo
     CDS             6927..7685
                     /locus_tag="ACFM4S_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            7659..8099
                     /locus_tag="ACFM4S_001326"
     CDS             7659..8099
                     /locus_tag="ACFM4S_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6050989.1"
                     /translation="MAKAALPDAVKHLLPDSVQALITVIGFNETLELVRLMGGTTYPL
                     RQGYTKNSQSRVAYLEEIIGSEAAGRLVEAMAPCNLFIPRCETALYELRNRKIRSQFD
                     RQTAGGTPAYEAVNDLALAHRLSDRHVWRILKQADKEAEQENLF"
     gene            8101..8622
                     /locus_tag="ACFM4S_001327"
     CDS             8101..8622
                     /locus_tag="ACFM4S_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050990.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            8765..9310
                     /locus_tag="ACFM4S_001328"
     CDS             8765..9310
                     /locus_tag="ACFM4S_001328"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6050991.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAANKTATGIEALSTPKNKRWCQ
                     VLGKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGATKV"
     gene            9307..>9505
                     /locus_tag="ACFM4S_001329"
     CDS             9307..>9505
                     /locus_tag="ACFM4S_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050992.1"
                     /translation="MKKSLIALCVAHCAKLKNDFGVPPLPEIKITPSPVRVGSLKQHP
                     SLRLGKSGVAAAKRAARKRKNR"
BASE COUNT         2481 a         1986 c         2639 g         2399 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatacgtcct agattcccac tttcgtggga
       61 atgacgggat gtgggttttt gtgcggattt gaaccggtaa gggtggtgtg ggattggtgg
      121 tttgcttagg atcttttgga ttgtattttg tatatacatt tacttgttga taaaagataa
      181 aataaaatta gaaactaaaa gtgagaaaaa attaataata atagggatgt ataaatgtaa
      241 aggctccgtt tcatagctaa ggttatctga atatatggaa aaaaagtaaa agtccataaa
      301 actaaaatat atagataatg ctaataataa tagaattatc actttcctag agtaaggata
      361 atattttttt atcgtaaaaa aatataaaat aggataaata attgccatat agatagattt
      421 tgtaacaaaa ttaaaaataa ataataaaaa taatgtaata aagtatattt tttggctatg
      481 aaataaaatt gtacataatt gaacgagcag atcaaaaaat gaactacata taacaataaa
      541 taataacgta tttaccataa taaatttaat aggtctcatt atcatattta ataaccactt
      601 catagtatag tggattaaat ttaaaccagt acagcgttgc ctcgccttgc cgtactatct
      661 gtactgtctg cggctttgtc gccttgtcct gatttttgtt aatccactat aaatgcagag
      721 tgggtggaaa cactcacttt atggtttgct acgctctgct caattagcaa cccgataacc
      781 caatatggat aatagggtaa ttaatccaat ctaatttgtc agtatccgtt aatttattgc
      841 aaaataaagt attgaattat gtcgggtgca aatgacgaaa tataagtttc cgtgcggacg
      901 gatcaagatt cccactttcg tgggaatgac ggtggaaaga ttgttgtttt tcccgatgaa
      961 ttcctgtgtt ttttgttttt ccggataaat tcctgtggct ttgagttttt tggatttcag
     1021 cctcaatgcc gtctgaacgc cgaatcgggc ttcagacggc attgcgtcat ttgaaattca
     1081 aaaccggcca gcctttttct ttggcttctt tttccagctc ggcatcgggg ttgacggcga
     1141 cgggttcgtc gacgaggcgc agcagcggca ggtcgttttt ggagtcgctg taaaaatagg
     1201 ttttgccgta gctttcgagc gtttcgccgc gttcggcgag ccattggttc aggcgggtga
     1261 ttttaccttc tttgaggctg ggcgtgccga tgtaattgcc ggtgtagcgg ccgtcggaac
     1321 cggattcgag ttgtgtgccg atgatgttgg taatgccgaa aaggcggcag acgggggtga
     1381 tgatgaactc gttggtcgat gagatgacga gggtttcgtc gcctgccatt tggtggctct
     1441 gcaccagcat acgctgcata ggcgagatgt gggggatgat gtattccgcc ataaattcgc
     1501 ggtgaaactc tgccagctct tctttgctgt aacgagcgag cggggcaagg tggaatttga
     1561 ggaatgcgtc gatgtcgagg caaccgtttt ggtagtcgcg gtagaatttt tcgttttgcg
     1621 cttcggtttc ggcagcgtca accaagcctt ttttgatgag gtattgcggc caggcgtggt
     1681 cggaatcggt gttgatgagg gtgttgtcga ggtcgaagat ggcgaggttt ttcattgggt
     1741 ttcctgttgt ttcaaaagct ggcgcaaaag cggcagggtg atgcgtttgc ccattgtgac
     1801 ggcgtagttg tccagcgtgt cgagcatcat catcaggctg tccatatcgc gccgccagtg
     1861 tttgagcagg tattcgaaaa tttcggaatc gacggttact tggcgtgccg ccgccatact
     1921 ggcgagcgcg tcgatttttt cttggtcggt taagggtttg acttcgtaaa cgaggcagta
     1981 cgccatacgc gtccgcaaat cttcgcggat gacaagctgc tggggcgtgt attccgaacc
     2041 gagcagcaaa aagcctttgc cgctgttgcg gaagcggttg aagatggaaa aaagcagggc
     2101 ttgttcttcg ttgcccagtt tttcgacttg atcgacggcg aggtattccg cctcgaacgc
     2161 ggcatcggtc agcggcatgg aggcggcatc gatataggcg gcgtttttgc cggcttcgag
     2221 cgcctgtgcg acccacgcct gcaaaagatg gcttttgccc gcgccttctt cgccccaaac
     2281 atagataaac tgtccgtgtt tgtgtcggag gacatagacc agttccgcgt tttccgtgcc
     2341 gaggaatttg tcgaaactcg gatagtcgtg tgcggcaaag tcgaaaataa gctggttcac
     2401 ggttcggcat tccgaggggt ggtaaacggg tttattgtac gttgttttcg cgcgcctttc
     2461 caatttgaac gatgccgtct gaaaacggct tcagacggca tcgttcagcc gcaggcagcg
     2521 ttgccgacat cgaggtgcat attgtggaac gcgttgagcg tgctgcggtg gccgatgctg
     2581 atgatgatgc tgtcgggcag tttttgtttc agtgcgcggt agagcagggc ctcggtcggt
     2641 tcgtccaaag cggcggtggc ttcgtcgagc aggacgattt tgggcttgga aagcagggcg
     2701 cggacgaagg cgacgcgttg cagttcgccc ggggagagtt tgtgttgcca gtcgtcggtt
     2761 ttatctaatt tatcaaccag ataacccaag cggcaggtgt tcatggcttc ggctaactcg
     2821 ggatgctgct tgtcaatgtc ggggtaacaa accgcgtcgc gcaggctgcc ctgtgccgtg
     2881 tacgggcgtt gcggcaggga gaggatgtct tgatgcggcg gacggctgac tttgccgctg
     2941 ctgccgaacg gccaaagccc cgccagcgcg cgcaacagcg aggttttgcc gcaaccgctc
     3001 gggccgcgta tcagcaggga atcgccgttt ttgaggttta tgttgatgcc gctcaacagg
     3061 atttcgccgt tgtggcagaa cagagcgacg ttttcctgtg ggggactgtt agtttttgca
     3121 caaggaacaa atagagtaaa aaaacgctga aatcttcgga agacgtggat ttcggcgttt
     3181 ttttgtatcc ggaaaagtta cgccagcttt ttcacaaaac cgcgccggaa tgcgcggttt
     3241 tctgtttaaa gctgacgaga ttagggaatt tttaaaactg ttttaagagg tttttaaaat
     3301 ggatttaatc aatactccgg ccataccact caacacggcc tatgatggcg atgtcgtctt
     3361 gggcattgct caaatctatt tcaaacggtg cgtaacgtgg attttcagac gttacaagca
     3421 gtttgcccgg tatacgttgc acacgtttga caaagaggtc attgcctata cgcaagacat
     3481 ataggccgtc acgcgggtca gtttcggcgt ggttgatgag aatggaatcc ccatgattga
     3541 gcacgccctc cattgaatcg cctttaacgg taattacaga cagtttttcc ggctgtttgg
     3601 tcacatagtt gtcaatccaa tatttccgga aagccaagca gaataaaggt tcttcgccga
     3661 agactggtgc gccataccct gctgctgcgg ctacgttgta gcgcggcacg aatacaaact
     3721 cggacaggtc gacgattgcc catagtgtcg gtgattccat cagaatttct acttacagag
     3781 aatgctccgg cgttttccgg cctggcttta tcgagatacg gcaagccttt tccggtcagc
     3841 agccagttta aatcacaacc ggtaacttct tgaattttta ctaggtaatc ggctgttggg
     3901 atagctccct ctttccaaac tctattaaat ccagaagccg acatttctat tttgttatag
     3961 atgtcagatg gcttagcccc atgaggccaa agaaatttga gcctatctaa aaaagtatcc
     4021 atagtaatcc taatttaact catttaagca aaacattaag caaaaaaaga aactcttttg
     4081 cttaaataag attactcaaa taatcaatat tttgtaaaaa taattacgtt tttgagaaaa
     4141 tattttagca aaagagtttc atgaagctgt tttgctaaat gtaattcact catttgctaa
     4201 atgacggcgg ttaataaacc tacttaatta aggaattgcg agaatgaaaa aaaagcaaga
     4261 aagcatgact gattggcatc gtgctgacat tgtggctcgt cttaaaaagg caggctggtc
     4321 tgtgagagcg ttatctatcg aggctaattt agcaccaaat acattaggga aagctttaga
     4381 tgctccttat ctgaaaggcg aaagaatcat tgcagcagcg attggagtac ccgcagaaga
     4441 aatctagcca tctcgttttg agaaacgaaa ccataagcca accttcccaa gatctataaa
     4501 tagataactg ttttgctaaa tagttccaaa agagtaccgc atttaagcaa aaatagaaag
     4561 cggaaaaaat gaaaatatct gcatctgata ttgcgaaatt aggaattccg agcctaccaa
     4621 ctgatagaca agggattgaa taccatgcca agaaaaataa ttggcaacac tgttttgagc
     4681 aaaaaggaag aggccgtcct aaaaaactgt atgaaatcgc ttccctccct gccgaaatcc
     4741 gagcagccat catgaaacgg cagtcggacg agctggcgga gaagatgccg aaaatgctgc
     4801 cccaagtcag accggggacg gcgatgtcgg ctcaggcact ggctgaagcg gccaagctgt
     4861 tgaacgagaa acaacggtcg gtggcggatg cgcgatgtgc ggtggtggcg gcggtattgg
     4921 ggattaaata cgaatacgat tgctctgcca aggctgcggt ggctcagttt ttgggcttgc
     4981 tggcagaagg taaattggac gcggtcacgc ttgggaactt ggaaaaggcc aatgaccgca
     5041 gccggtcggc gaaggttggc gaacgtactt tagacggctg gatttctgct tatttgaaag
     5101 cggaaaacgc gatagagcgg ttggttgctt tggctccgaa gacgacgaag gcggtcaagc
     5161 cgattgagag ctacggttgg ttgccgatgt ttatgcggtt tcacaacatc ccttcggctc
     5221 cgaaactggc gcacagctac cgccggtttg tgcagtgggc ggaagcggaa aatatgccgg
     5281 tcaatgatgt gcctaatttg agtatggtgc ggcgcgtttg ggacaagctc ccgctaatta
     5341 tgcaggagcg cggcaggaaa acgggggcgg cttataaatc gctgctgcct tatgtgaaac
     5401 gtgattgggg ggctttgaag ccgaacgatg tttggatcgg cgacggccac agctttaagg
     5461 cgaaggtggc acaccctgta cacggcaggc cgtttaagcc ggaagtgacg gtgattattg
     5521 atggttgtac gcggtttgtg gtcgggtttt cggtttcgtt ggctgaaagt tgtgtggcgg
     5581 tatcggatgc tctgcgtatc ggggtcaagc attttggttt gccgattatc tattactcgg
     5641 ataacggcgg cggccaaacc ggcaagacga tagaccatga aatcacgggt attacgtccc
     5701 gactgggtat ccgccatgaa acgggtatcg cgggcaaccc gcaaggtcga ggcatcatcg
     5761 agcgatggtg gaaagacaat ctgattgaga tggcgcgcca gtatgagacg tttgcgggcg
     5821 caggaatgga cagtagcacg aagaatctga tgtaccgcaa gatggaaagt gcgtttaacg
     5881 ctttggaaaa aggcaaggat ttaacggagg aacagcaaaa atatttgaaa aaactgccga
     5941 gctggtcgcg ttttatcgcg gatgtggtca agtgtatcga tgattacaac aaccgtccgc
     6001 acggcgagct gccccgacat ccggacggcg ggcattattc gccgaaggct tatcgggaaa
     6061 taaggctgga acaggacggt atcgcgccgg atatgttgtc ggcggaagag ctggcgacga
     6121 tgtttatgcc gcaggaagtg cgaaaggtac agcgcggttg gctggatttg ttcagcaact
     6181 cttatttctc aaccgagctg gcggagtatc acaaagacga ggtacgggtc agctacgatt
     6241 tgagcgatgc gtcggcggtc aatgtgtttg atatggacgg caagtttatt actaaggcgc
     6301 aggccaacgg caatacccgc gaggctttcc cgacagcgcg tatcgaccaa ctggcggaaa
     6361 aacgccgaaa aggcaaaatc aagcgggcgg aaaatgcaat caagctcgcg aatgcggaag
     6421 tcaatccggc actggaacag gcggcagctt gggacgagct gggacatttg ggcggaaacg
     6481 tcatcgaggc ggagtatgcg gtattgccga aaacgggcac ggacgacggg attttcttgt
     6541 ttgaggcgga tatgtaaagg aaaacatgat ggaaaaacag aaaaatgcgg cgttttcggc
     6601 cgagcttgtt gaaaaattga aactcaagcg agcccttggg cggattcaac gatttcaagc
     6661 aaagattcaa ggtgttcccg ctgaacggaa tcaggctcaa acgtttttgc ctgcgcttga
     6721 aggaaactgc gaacctgctc aatcgaagtc ggctcttgac gggtaatccg ctggagcagc
     6781 caggcaagta cgaaggaatc ggtaagcgac ctttcttcca agtcttgaac ggcgacttcc
     6841 agcatgatca ggcgtttttc caaatcggga aactctttca tttcagacgg cctttaaagg
     6901 ttgtttaaaa ctcaaggata ttaaaaatga aacaaattaa tcaagcatta caacaaaaac
     6961 tggttgaatt taaagaaaaa tcgggtatga accaaaccca actggcacgc ggtatcgata
     7021 cttcgccggc atccatcagt atgtatctga acggcactta tgcggaaaaa ggcggcaatt
     7081 atgaaaccat cgagacgaaa atcgaggcgt ttttggagat gcagaaaagt aaggcgcgac
     7141 gcgaagaact ggtgttgggt tttgtagcga ctaagacgac ccgccgaatc gcggaagtga
     7201 tgcgcaatgc gcacgaaggc ggcgaaacag tggtgatcta cggtcaggcg ggattgggaa
     7261 agactcaggc ggtcaaaaac tactgcgaga aaaacccagc ggccatcttg attgaggcta
     7321 atccgagctt tacggctttg gtcttgatgc gcaagttggc gacggcggcg aaggtatcgg
     7381 cgatgggcag cctgaatgat ttgtttgagt ctgtatctga ccgcctgcgc gattcgggcc
     7441 gtctgattgt ggtcgatgaa gcggaaaacc tgcccttgcg cgcccttgag attatccgcc
     7501 gactgcatga tgaaacgggc tgcggcttgg tgttgagcgg tatgccccga ctggtggcca
     7561 acctgcgcgg taagcatggc gagctggtgc aactttatag ccgcgtgtct gttgcgctga
     7621 atttgggcga atctttgccg gatgacgagc tctttgagat tgcgaaagcg gctttgcctg
     7681 atgcggtcaa gcatctgctc cctgatagtg tacaagcgtt gattacggtc atcgggttta
     7741 atgaaacgct ggagctggtg cgcctgatgg gcggtacgac ttatcctttg cggcagggtt
     7801 atacgaaaaa cagtcaatcc cgtgttgcat acttggaaga gattatcggc agtgaggcgg
     7861 ccggtcggct ggtggaggca atggctccgt gcaatctgtt tataccccgt tgcgagacgg
     7921 ccttgtatga gttgcgaaac cgtaaaatcc gcagtcagtt tgaccggcag acggcaggcg
     7981 gtacccctgc ttatgaggcc gttaacgatt tggccttggc acaccgccta agcgaccgcc
     8041 atgtgtggcg aattttaaag caggcggata aggaagcgga gcaggagaat ttgttttaga
     8101 atggaatgcc atgcagatgt atggcatttt attttggaga aaaatatgaa aaagttttat
     8161 tttgtgctgc tggcgttggg tttggcagcg tgtgggcaag aacaatcgca gaaagctgat
     8221 gcggagcagt atttttttgc caataaatat caatttgcag atgagaaaca ggctttttat
     8281 tttgaacgcg ccgcccgttt ccgtgtattg caacaaggcc ttggcgggga ttttgagagg
     8341 tttttaaaag gagaaatacc taatcaagaa aatcttgcaa agtatcgtga aaatattact
     8401 caagcagtcg cttattatgc ggacacgaat ggagatgatg acccataccg cgtctgcaaa
     8461 caggctgcgc aagatgcaga aatcctgatg aagagtatgg taacaagcgg tggaggcggt
     8521 acaactgatt tagataagga aagttatcaa aattaccgaa aatcaatgca agaatgccgt
     8581 aaaacaataa cggaagctga agccaatttg ccgaaaaaat aaaataaacg attctaaggc
     8641 cgtctgaaca acaggcggct tttttgttgc ctactgacac tgtttcgccc gctgcaaaag
     8701 ccatgccgtt tgaaaatgta agcctctgaa agtgcatttt aatctgattt tgagggaggc
     8761 tttaatgagc aaaattattt gtctgactgc cggacacagt aacaccgacc cgggcgcagt
     8821 caacggcagc gaccgtgagg cggacttggc gcaggatatg cgcaacattg tggcttcaat
     8881 cctgcgtaac gattacggcc tgaccgtacg caccgacggc gaaggcaaag gcaatatgcc
     8941 cttgcgcgat gcggtcaagc tgattcgcgg ctcggatgtg gcgattgagt tccacaccaa
     9001 tgcggcggcg aacaaaacgg cgacaggcat cgaagccttg tccacgccga aaaataaacg
     9061 ctggtgtcag gtgctgggca aagccgttgc caagaaaacc ggctggaaac tgcgcggcga
     9121 agacggcttt aagccggata acgcagggca acattcgcgc ctggcttatg cgcaggcagg
     9181 cggcattgtg tttgagccgt tttttatcag taacgacact gatttggcct tgtttaagac
     9241 gaccaaatgg ggcatctgcc gcgcgattgc ggacgcgatc gcgatggaat tgggagcgac
     9301 gaaggtatga aaaagtcttt gattgcttta tgtgttgccc attgtgcaaa gttgaaaaac
     9361 gattttggcg taccaccgtt acctgaaatc aaaatcacgc caagccctgt tcgggtaggc
     9421 tctttgaaac aacatccgag cctgcgcttg ggtaaatcag gcgtggcagc cgcgaaacgt
     9481 gcggcgcgca aacgcaagaa tcgtc
//
LOCUS       DAXHMX010000078         9432 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHMX010000078 DAXHMX010000000
VERSION     DAXHMX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.078"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(134..1576)
                     /gene="aldA"
                     /locus_tag="ACFM4S_001330"
     CDS             complement(134..1576)
                     /gene="aldA"
                     /locus_tag="ACFM4S_001330"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6050993.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGRADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1611..1811)
                     /locus_tag="ACFM4S_001331"
     CDS             complement(1611..1811)
                     /locus_tag="ACFM4S_001331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050994.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1820..2209)
                     /locus_tag="ACFM4S_001332"
     CDS             complement(1820..2209)
                     /locus_tag="ACFM4S_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050995.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTVDRISVSFIREIQAA"
     gene            complement(2210..2386)
                     /locus_tag="ACFM4S_001333"
     CDS             complement(2210..2386)
                     /locus_tag="ACFM4S_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6050996.1"
                     /translation="MSGYVQFLGTDSKGQSKFIFVGTNENGSITTIHTKSGKDFWRTL
                     NNNPKNKTIYPKAR"
     gene            complement(2417..3334)
                     /locus_tag="ACFM4S_001334"
     CDS             complement(2417..3334)
                     /locus_tag="ACFM4S_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6050997.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRRRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWNLSPNLRGSAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFNRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3506..4306
                     /locus_tag="ACFM4S_001335"
     CDS             3506..4306
                     /locus_tag="ACFM4S_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6050998.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4355..5131
                     /gene="mlaE"
                     /locus_tag="ACFM4S_001336"
     CDS             4355..5131
                     /gene="mlaE"
                     /locus_tag="ACFM4S_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6050999.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5182..5676
                     /gene="mlaD"
                     /locus_tag="ACFM4S_001337"
     CDS             5182..5676
                     /gene="mlaD"
                     /locus_tag="ACFM4S_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6051000.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5713..6303
                     /locus_tag="ACFM4S_001338"
     CDS             5713..6303
                     /locus_tag="ACFM4S_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6051001.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6360..6638
                     /locus_tag="ACFM4S_001339"
     CDS             6360..6638
                     /locus_tag="ACFM4S_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6051002.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6635..7462
                     /locus_tag="ACFM4S_001340"
     CDS             6635..7462
                     /locus_tag="ACFM4S_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6051003.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7587..7949
                     /locus_tag="ACFM4S_001341"
     CDS             7587..7949
                     /locus_tag="ACFM4S_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6051004.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7949..8332
                     /locus_tag="ACFM4S_001342"
     CDS             7949..8332
                     /locus_tag="ACFM4S_001342"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6051005.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8329..8817
                     /locus_tag="ACFM4S_001343"
     CDS             8329..8817
                     /locus_tag="ACFM4S_001343"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6051006.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8898..9026)
                     /locus_tag="ACFM4S_001344"
     CDS             complement(8898..9026)
                     /locus_tag="ACFM4S_001344"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6051007.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(9032..9412)
                     /locus_tag="ACFM4S_001345"
     CDS             complement(9032..9412)
                     /locus_tag="ACFM4S_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6051008.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGAG"
BASE COUNT         2171 a         2640 c         2378 g         2243 t
ORIGIN      
        1 taaatttaat ccgctataca aagacaaaaa catcgacctc ggtcatgact gattgccggt
       61 gaaactgtaa aaatgccgtc tgaacctgaa aacgggttca gacggcattt tctatcgggg
      121 ttttaaagcg gcattaaatg tcggtttcca gataaacgac ttgggtttgc agatattctt
      181 ccaaaccgtg tttgccgtcc gcgccgccga tgccggattt tttccagccc gcgtggaagc
      241 cttgcatggc ttcaaagttt tcgcggttga tgtaggtttc gccgaattgc aggcggcggg
      301 taacgtagaa ggcttcgttt aaattagtcg tataaacaga actggtcaga ccaaactcgc
      361 aatcgttagc cagtgcgatg acttggtcaa gcgtgtcgaa agcggaaacg ggcagcacgg
      421 ggccgaaggt ttcttctttc ataatgtcca tactgttgtc ggtgtcggtc agcagggtcg
      481 gctcgaagaa ataaccgcgt ccttcggcgc gtttgccgcc gcagaccagt ttcgcacctt
      541 gtttgactgc ccgttccact ttttcggcaa cggctttgac ggcgcgctct tcaatcagcg
      601 gacccatttc cagcgcgcct gcttcggctt cggcagggtt gccgtagcgc acgcctttca
      661 tggcggcagt cattttttca atgaatgcgt ctttcagact gctgtggaca taaacgcgct
      721 cggcgcagtt gcagatttga ccggtgttgc cgacacgcga agccaagatg gatttcaccg
      781 ccaaatccaa atccgcgtct ttcaaaacga tggcgggggc tttgccgccg agttccaacg
      841 aaactttggt gatgttggcg gaagcggctt ccatcacttg gcggcccgct tcgacggagc
      901 cggtcaggct gaccatatcg acttgcggat gggcggacaa ggcattgccg atttccgcac
      961 ccggaccgtt caccacgttg aacacgcctg cgggcaatcc gaccgcatcg acgatttcgg
     1021 cgaagatgtg gcagttgatc ggggttacgc tgctgggttt gacaacgatg gtgttgcccg
     1081 ttactaaggc gggacccatt ttgcgggcaa tcaggaagaa agggaagttc cacggcaaaa
     1141 tgccggcaat tacgcccagc ggacgtttga acaataaaat gttttcgcgc gggcggtcgc
     1201 tttggatgat ttcgccttcg taacggcgcg cccattcggc ttggtaatcg agatagtcgg
     1261 cggtgaacat cacttccacg cgcgccaagt ctttggtttt gccgccttcg gcaacgatgg
     1321 tgtcggtcag ctcgtcggcg cgttcgcgta tgccttgggc gattttgcgc aaatacgcgc
     1381 cgcgttcgac cgcaggcaga cgctcccaag ccggttgcgc cgcacgcgcc gccgctacgg
     1441 cgcggtcaac gtccgccctg ccgcctttgg gttcgcgggc gatggcctct tcggtggacg
     1501 ggttcaatac gtcgcgccat tcgccgttga aatcgttttc aaagcgtccg ttgatgtaca
     1561 tggccaattg tttcatttcg ggttctccag ttttgtagtc agatgtagtt ttagtttatt
     1621 cccaaataaa ttaccgtaca agtttctttt gaaatgttta gacgagaagc tactatgtct
     1681 gcatattgtt ctgctaattt atattcactc gaatgcaact gccgattatt ccaatctgca
     1741 ttcgcaccag taaaagcccc agcattgccg cctgtggcat aaccaatcgc taaactgcca
     1801 acggtatcca caacaggttt caggctgcct gaatttctct aataaatgaa acacttattc
     1861 tatcaaccgt taatgtaacc aaacaattat caagataaga gtagtttatt aaatactcat
     1921 cttcttcaaa ttgtatattt tgtagggtta gatgattatt ttttaattct gccgttattt
     1981 catacttgaa actatcatct atttttctaa atccaatatt tgaagttgat ttatccagta
     2041 tgtaatcatt aaagatttca taggataatt ccacttcata aaattgattt tcaaatattt
     2101 tgaatgggca gtacgatata aaacaatgta gttcataacc atttatttca atcaatactg
     2161 cctcttcaat ggtgttatct aactttttga ttttacattt atataacatt tatcttgcct
     2221 ttggataaat tgttttgttt tttggattgt tattcaaagt tctccaaaag tcttttcctg
     2281 attttgtatg tatcgtggta atactaccat tttcattggt tccaacaaaa ataaatttag
     2341 attgaccttt ggaatcagta cccaaaaact gaacatatcc agacatgcgg atttcgggtt
     2401 ttaaatcaaa tgcggcttat cggttttctt ttttggtttc cgcaggcgtg ctgccgaaat
     2461 attgcctgaa tgcctgagta aagctggaaa cgtggcggta gccgcacaaa tacgcggttt
     2521 cgcctatgct tttgcccccg ttttgcaaca ggtagagcgc gtgctgcatt tgtttgtggt
     2581 gcagccactc gcttgcggta ataccgaaat ggtcgcgcat acggcgttgc agcgtccttt
     2641 cactgatgtt cagcgcgtct gtcagccggt tgacttggtg tgcgccgtcg gcaaacgcgg
     2701 catttagggt gcggttgaag tcttcagacg gcatagcgtc tgcttccgcc gtttgccccg
     2761 ccgccggccc gatgccgtct gaaaccgtgt cccacaagtc cgacagcagc cgcaaaacgt
     2821 ccgcctcgcg gcgcaatgtt tcgcccaaat gcccctttgg gacagctttc aggcaggatg
     2881 ccgccgagcc gcgcaggttt ggggacaaat tccatattct gaccggttcg cggtaaagca
     2941 ggggtgcgaa acgcgcatac tccggacgca gcagccattg ttccatacct ttaatggtca
     3001 ttttgaccgt tttgccgcct cggtaaagat agcggctgaa caggacctct tccccgacag
     3061 caattaggac gatcttgccg ccatccgcat cgatttggaa gcggcggcgg ttgatgccga
     3121 agtccaaact gccttccagc aagagcacga acgacacata aggttcggcg aggcggctgc
     3181 tgcaaaaatc gcaccgtgcc gttaccgtgc cgccgtggat ggaaatgccg ttggacaaag
     3241 tatcgaaacg gtagctgccc tccacatagc tgccgccgcc gcttttgacc agattgacga
     3301 accggctgct gttcaaatgt tctgcttttt ccatagtttt ctgataaagg ttatcatttg
     3361 aaagataaca tttttcgcca cagcaaacaa tccgccgcca caacggcatt ttcacaacat
     3421 ttaccgtccg tatgccgtct gaaaattgcc cgtcttcccg ataagcctta aaatacacgc
     3481 cgtcaaacct accgtccgtc cgtacatgag cccatccccc tttatcgaaa tgaaagacgt
     3541 cgccttcgcg tatggcgacc gcccgattct gaagaacatc aatttcagca ttccgcaagg
     3601 caattttgct gccgtaatgg gcggttcggg cagcggcaaa accacgctga tgcgactgat
     3661 tacaggacag attcgtccgc agtccgggca ggttttgatt gaaggacggg atttggcggg
     3721 cttttcggct gacgagttgt atgaacaccg ccgccgtatg ggcgtattgt tccaacacgg
     3781 cgcgctgttt accgatttgt ctgtatttga caatattgct tttccgatgc gcgaactgac
     3841 gcgcctgccg gaagcggtga ttcgagattt ggttttgttg aaactgaacg cggtcgggct
     3901 gcgcggcgtg gaaaacctga tgccgtccga gttgtccggc gggatgtcgc gccgcgtcgc
     3961 gcttgcccgc acaatcgcgc tcgatcccga aattatgttg tacgacgagc cgtttaccgg
     4021 tctcgatccg atttccttgg gcgtgattgc ccacttgatc agccgcgtca acaaggcttt
     4081 gcgttcgacc agtattatgg tcacgcacga cattgaaaaa tctttggaaa tcgtcgatca
     4141 ggtgattttc ttggcgcacg gcgaaattat gttctccggc tcgccgcagg aaatgcgcga
     4201 actggattcg ccttgggtgc gccagtttgt cggcgggctg gcagacggcc ccgtggcata
     4261 ccgttatccg gcgcaaacgt cgttgcagca ggatttgctc gggtaaattg agccggtgcc
     4321 gtctgaaacc gttgaacttt acatatagaa atttatgaat tttatccgtt ccgtcggggc
     4381 gaaaaccctc ggccttattc aatccctcgg cagtattacg ctgtttctgc tgaacattct
     4441 ggcgaagtcc ggtacggctt tcgtccgtcc gcgcctgagc gtgcgccaag tgtattttgc
     4501 cggcgtgctg tcggtgctga ttgttgccgt ttcggggctg ttcgtcggca tggtcttggg
     4561 tttgcagggc tatacgcagt tgtcgaaatt caaatccgcc gatattttgg gctatatggt
     4621 tgcggcttcg ctgttgcgcg aactgggtcc ggtgttggcg gcgattctgt ttgccagcag
     4681 cgcgggcggt gcgatgacca gcgaaatcgg tttgatgaaa acgaccgaac agctcgaagc
     4741 gatgaacgtg atggcggtaa accccgtcgc ccgagtggtt gcgccgcgct tttgggcggg
     4801 cgtgttttcc atgccgcttt tggcttcgat tttcaacgtg gcgggtattt tcggcgcgta
     4861 tttggtcggt gtaacctggc tgggcttgga cagcggtatt ttctggtcgc aaatgcagaa
     4921 caatatcacg atacattacg atgtaatcaa cggtctgatt aaatccgccg cgttcggcgt
     4981 ggcggtaacg ctgattgccg tgcatcaggg cttccattgc gtcccgactt cggaaggcat
     5041 tttgcgcgcc agcacgcgca cggtggtttc gtccgccctg acgattttgg cggtcgattt
     5101 tatattgacc gcgtggatgt ttacagattg acaggccgtc tgaagacgaa acgcggaaca
     5161 tcggatattc aaggaacttt aatgaaaaag aacatattgg aattttgggt cggactgttc
     5221 gtcctgattg gcgcggcggc ggttgccttt ctcgctttcc gcgtggccgg cggtgcggcg
     5281 ttcggcggtt cggacaaaac ctacgccgtt tatgccgatt tcggcgacat cggcggtttg
     5341 aaggtcaatg cccccgtcaa atccgcaggc gtattggtcg ggcgcgtcgg cgctatcgga
     5401 cttgacccga aatcctatca ggcgagggtg cgcctcgatt tggacggcaa gtatcagttc
     5461 agcagcgacg tttccgcgca aatcctgact tcgggacttt tgggcgagca gtacatcggg
     5521 ctgcagcagg gcggcgacac ggaaaacctt gctgccggcg acaccatctc cgtaaccagt
     5581 tctgcaatgg ttctggaaaa ccttatcggc aaattcatga cgagttttgc cgagaaaaat
     5641 gccgacggcg gcaatgcgga aaaagccgcc gaataaacgt atgccatcat attcaaacta
     5701 ggacacttaa tcatgaaaaa atcctccctc atcagcgcat tgggcatcgg tattttgagc
     5761 atcggcatgg catttgccgc tcctgccgac gcggtaaacc aaatccgtca aaacgccact
     5821 caagtattga gcatcttaaa aagcggtgat gccaacaccg cccgccaaaa agccgaagcc
     5881 tatgcgattc cctatttcga tttccaacgt atgaccgcat tggcggtcgg caacccttgg
     5941 cgcaccgcgt ccgacgcgca aaaacaagcg ttggccaaag aatttcaaac cctgctgatc
     6001 cgcacctatt ccggcacgat gctgaaatta aaaaacgcca acgtcaacgt caaagacaat
     6061 cccatcgtca ataaaggcgg caaagaaatc atcgtccgcg ccgaagtcgg cgtacccggg
     6121 caaaaacccg tcaacatgga cttcaccacc taccaaagcg gtggtaaata ccgtacctac
     6181 aacgtcgcca tcgaaggcgc gagcctggtt accgtgtacc gcaaccaatt cggcgaaatc
     6241 atcaaagcga aaggcgtgga cggactgatt gccgagttga aggctaaaaa cggcagcaag
     6301 taatccataa gccatgccgc cagactgaag atggcatgga ttttttccta ccgaacacta
     6361 tgcatacaga actcaacaac ggaacactgc acatcagcgg cgacgtaacc gtcaaaaccc
     6421 tgaccgccgc cgcctttacc cgcttcagac agcaatgtcg tctgaaagac acccgcgcgg
     6481 ttgatttgag cggcgtcgga cgcgccgatt ccgcctgcgt gtcgctgctg ctcgaagtgc
     6541 tgcgcggttg caaaggcagc gtcaggctga ccggcattcc cgaatccgtg cgcgcgctgt
     6601 ccgaactgta cgaaatcaaa gactggctga aatcatgaaa aaaaccgcct atgccttcct
     6661 cctgctgatc gggttcgctt ccgcccctgc atttgccgaa acccgccccg ccgaccctta
     6721 tgaaggctac aaccgcgccg ttttcaaatt caacgaccaa gccgaccgct acattttcgc
     6781 ccctgccgcg cgcggctacc gcaaagttgc gccgaaaccc gtccgcgccg gcgtgtccaa
     6841 tttttttaac aacctgtgcg acgtggtcag cttcggcagc aatatcttgc gcttggacat
     6901 caaacgcgca agcgaagacc ttgtccgcgt cggcatcaac accactttcg gtttgggcgg
     6961 gcttatcgac atcgccggcg cgggcggcat tcccgacaat aaaaacacct tgggcgacac
     7021 gtttgcttcg tggggatgga aaaacagcaa ttatttcgtg ttgcccgtct tagggccgtc
     7081 caccgtccgc gacgcgctcg gcacgggtat tacctccgtt tattcgccca agaatatcgt
     7141 cttccgcacc cctgtcggac gctggggcac gactgccgta tccgccgtca gtacgcgcga
     7201 aggtctgctc gatttgaccg acagtctgga cgaagccgcc atcgacaaat acagctacac
     7261 gcgcgacctc tatatgaaag tccgtgcgcg gcagaccggt gcaacacctg ccgaaggtac
     7321 ggaagataac atcgacatcg acgaattggt cgaaagtgcc gaaaccggcg cggcggaaac
     7381 tgccgttcaa gaagattccg tatccgaaac acaggcagaa gcagcagggg aagccgaaac
     7441 gcaacctgga acacaacctt aaagccatgc cgtctgaaaa tccgtgttcg gacggcattc
     7501 ccttctgtcg gaacggacag tccgaaacca cagattgggc tataatctct tttttgcgca
     7561 taccgcgcga cagcaaagga aaaaccatgt acgaagtcaa ccgcagcgta ttcgtcctca
     7621 tccctttaga acctttttgg aactggctgc aaaccctgcc gggcaaccac ctcgacggac
     7681 tgactcttga agacatccaa gccgacgcca attcctacct tgtccgcccg tgcgaaaccg
     7741 ccgacgaagt atgggacgaa atcgaagccc gttttgaaga cattttcgcc gccgaacttg
     7801 ccgactggtg cgaagacgaa cgcgagtggc ctgcgctcga tgccgacatt ttcaacgaat
     7861 ggttcgacat ccagctttct accgtcatca ccgacctcga acacgaaccg cttgcccgcg
     7921 aagccttcca acccatcaac ctgaactgat gaagctgacc gtccgcaact accatctcga
     7981 cggctacggc catgtcaaca atgcgcgcta cctcgaattt ttcgaagaag cgcgctgggc
     8041 gttttttgaa gaacgcggac tgctgcacga gttggcaggc ctcatactga ttgtcgcccg
     8101 catcgacatc cgatacagcc gccccgccgt cgaaggcgac gtattgcagt tttcatgccg
     8161 cctgaaaacc cccggcacgc gccgtattgt gctgacccag acgattacac tgccaaacgg
     8221 caaaaccgcc gcagaagccg acatcaccct gatgcccgtc cacgccgcca cacaacgcac
     8281 cgtcagcctg cccgccaccc ttgcccgcgc attggaagcc ttgtccgaat gaaaaaaata
     8341 ctcaccgccg ccgtcgtcgc actgatcggc atcctccttg ccatcgtcct catccccgac
     8401 agcaaaaccg cgcccgcctt ctccctgccc gacctgcacg gaaaaaccgt ttccaacgcc
     8461 gacctgcaag gcaaagtaac cctgattaat ttttggtttc cctcctgtcc gggttgtgtg
     8521 agcgaaatgc ccaaaatcat taaaacggca aatgactata aaaacaaaaa cttccaagtc
     8581 cttgccgtcg cccagcccat cgatccgata gaaagcgtcc gccaatatgt caaagactac
     8641 ggtttgccgt ttaccgtcat gtatgatgcg gacaaagctg tcggacaggc gttcggcaca
     8701 caggtttatc cgacttccgt ccttatcggc aaaaaaggcg aaatcctcaa aacttatgtc
     8761 ggcgaacccg atttcggcaa actctaccaa gaaatcgata ccgcgctggc gcaatagcga
     8821 tgccgtctga agcgcaaaaa acagaaacga tgttcctgtt ttttcagatc atccgccccg
     8881 acacgttcag acggcattca accttccggc agattctttt tcaccactgc cggattgcct
     8941 gccgccaaag agtagggcgg aatgtctttt gtaaccaccg cgcctgcgcc cacgaccgaa
     9001 ccgcgtccga cggttacgcc cgccattaca atcaccctgc gccccggcca gacatcgtcc
     9061 tccaacgtaa tcggacggat ttccgtgtag ccctcaaagc gttttttgaa cggtcaaact
     9121 tgtggttatt tgaataaaac agacattccg gctccatcat cacatttttg ccgaccaccg
     9181 gcccacggca gatttcacag tttgccccga tgcccgagcc gtcgcccaaa accgtatccg
     9241 gaaacacata cgccccgcgt tcgatattga ccccgcgtcc gatatgcgga gaaacccgcc
     9301 gcgccaaaaa accgcgtacc cgtctgccga cgatgccgat gccgcgcata tgtgacggcg
     9361 gcaacaccga accggcaaaa cgcagcagca ggaaaaagaa ttttgatccc atcaaacctc
     9421 cctcaaaaga ac
//
LOCUS       DAXHMX010000079         9321 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHMX010000079 DAXHMX010000000
VERSION     DAXHMX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.079"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            164..1507
                     /locus_tag="ACFM4S_001346"
     CDS             164..1507
                     /locus_tag="ACFM4S_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6051009.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1510..2742
                     /locus_tag="ACFM4S_001347"
     CDS             1510..2742
                     /locus_tag="ACFM4S_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6051010.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2735..3511
                     /locus_tag="ACFM4S_001348"
     CDS             2735..3511
                     /locus_tag="ACFM4S_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6051011.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSIIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKNTDISKTFAERIEQRVVDLATGEYVADAPKDFSARVAGKDPA
                     QSIQIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3511..4137
                     /locus_tag="ACFM4S_001349"
     CDS             3511..4137
                     /locus_tag="ACFM4S_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6051012.1"
                     /translation="MADMKRLKHLMFSPFINNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4141..4734
                     /gene="nqrE"
                     /locus_tag="ACFM4S_001350"
     CDS             4141..4734
                     /gene="nqrE"
                     /locus_tag="ACFM4S_001350"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6051013.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4748..5965
                     /gene="nqrF"
                     /locus_tag="ACFM4S_001351"
     CDS             4748..5965
                     /gene="nqrF"
                     /locus_tag="ACFM4S_001351"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6051014.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPSHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6117..7172
                     /locus_tag="ACFM4S_001352"
     CDS             6117..7172
                     /locus_tag="ACFM4S_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6051015.1"
                     /translation="MPSETRLPNFIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRGKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAYVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7198..7413
                     /gene="nqrM"
                     /locus_tag="ACFM4S_001353"
     CDS             7198..7413
                     /gene="nqrM"
                     /locus_tag="ACFM4S_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6051016.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7537..8115)
                     /gene="grpE"
                     /locus_tag="ACFM4S_001354"
     CDS             complement(7537..8115)
                     /gene="grpE"
                     /locus_tag="ACFM4S_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6051017.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8299..9117
                     /gene="cysE"
                     /locus_tag="ACFM4S_001355"
     CDS             8299..9117
                     /gene="cysE"
                     /locus_tag="ACFM4S_001355"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6051018.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2263 a         2586 c         2301 g         2171 t
ORIGIN      
        1 aacagcttcc gcaaggaaaa aagcagcaaa tctgacaaac aggtacaatt cctgatagaa
       61 tctaacactt tgctacatta atttattcaa tgcttttgat gccacgcgac acggtatcaa
      121 aacacgccgt ttttacctta accatcacaa gtaatgcaaa tatatgatta aaatcaaaaa
      181 aggtctaaac ctgcccatcg cgggcagacc ggagcaagtc atttatgacg ggcccgtcat
      241 taccgaagtc gcgttgcttg gcgaagaata tgccggtatg cgcccctcga tgaaagtcaa
      301 ggaaggcgat gccgtcaaaa aaggccaagt gctgtttgaa gacaaaaaga atccgggcgt
      361 ggtgtttacc gcgccggttt caggcaaaat cgccgccatc catcgcggcg aaaagcgcgt
      421 gcttcagtcg gttgtgattg ccgttgaagg caacgacgaa atcgagttcg aacgctacgc
      481 gcccgaagcg ttggcaaact taagcggcga agaagtgcgc cgcaatctga ttcaatccgg
      541 tttgtggact gcgctgcgta cccgtccgtt cagcaaaatc cctgccgtcg atgccgagcc
      601 gttcgccatc ttcgtcaatg cgatggacac caatccgctg gcggcagacc ctgtggttgt
      661 gatcaaagaa gccgccgagg atttcagacg aggtttgctg gtattgagcc gtttgaccga
      721 gcgtaaaatc catgtgtgta aggcagctgg cgcagacgtg ccgtctgaaa atgctgccaa
      781 catcgaaaca catgaattcg gcggcccgca tccggccggt ttgagtggca cgcacattca
      841 tttcattgag ccggtcggtg caaacaaaac cgtttggacc atcaattatc aagatgtaat
      901 tgccatcgga cgtttgtttg caacaggccg tctgaacacc gagcgcgtga ttgctttggg
      961 tggttctcaa gtcaacaaac cacgcctctt gcgtaccgtt ttgggtgcga aagtatcgca
     1021 aattactgcg ggcgaattgg ttgacgcaga caaccgcgtg atttccggtt cggtattgaa
     1081 cggcgcgatt acacaaggcg cgcacgatta tttgggacgc taccacaatc agatttccgt
     1141 tatcgaagaa ggccgcagca aagagctgtt cggctgggtt gcgccgcagc cggacaaata
     1201 ctccatcacg cgtacgaccc tcggccattt cctgaaaaac aaactcttca agttcacgac
     1261 agccgtcaac ggtggcgacc gcgccatggt gccgattggt acttacgagc gcgtaatgcc
     1321 gctggacatc ctgcctaccc tgcttttgcg cgatttaatc gtcggcgata ccgacagcgc
     1381 gcaggcattg ggttgcttgg aattggacga agaagacctc gctttgtgca gcttcgtctg
     1441 cccgggcaaa tacgaatacg gcccgctgtt gcgtaaggtg ctggaaacca ttgagaagga
     1501 aggctgatta tgggcttgaa acattttctg gaaaaaatcg aaccgcactt cctgccgggc
     1561 ggcaaacatg aaaaatggta tgccctctac gaagctgcgg cgacgatttt ctatacatcc
     1621 ggtgcggtaa cgcgcaaagc ggcacacgtc cgcgatgcgc tcgactccaa acgcatgatg
     1681 attttggtgt ggctggcttt gttccctgcc atgttctacg gtatgtacaa cgtcggcgca
     1741 caggcattcg gtgcgttaac gcccgatttg ctgcaacaaa gcatcgccaa cgactggcat
     1801 tacgcccttg ccaacgcttt gggcatcaat atgtcgtctg aagcgggcgt gttgggcaaa
     1861 atgctgttcg gcgcgattta cttcctgccg atttacgcga ccgtatttat tgtcggcggt
     1921 ttctgggaag ttttgttcgc caccgtgcgc aaacatgaaa tcaacgaagg tttctttgtt
     1981 acctcgattc tgtttgcctt aatcgttccg cccacgctgc cgctgtggca ggcagctttg
     2041 ggtatttctt tcggcgtggt ggttgcgaaa gaagtattcg gcggtacagg taaaaacttc
     2101 atgaaccctg cgctggcagg ccgtgccttc ctgttcttcg cctaccctgc caacttgagc
     2161 ggcgatgcgg tttggacggc ggttgacggc tattccggcg caaccgcgct ggcgcaatgg
     2221 gcggcacacg gtgcagacgg cctgaaaaac gccgtaaccg gtcaaaccat cacttggatg
     2281 gatgcgttta tcggcaaact gcccggctcc atcggcgaag tatccacttt ggcactctta
     2341 atcggcggcg cgtttatcgt gtttgcccgc atcgcttctt ggcgcattat tgccggcgtg
     2401 atgatcggta tgattgccat gtcttcgctg ttcaacttca tcggttcgga caccaacgct
     2461 atgtttgcta tgccttggta ctggcatttg gtcgtcggcg gcttcgccat cggtatgctg
     2521 tttatggcaa ccgaccccgt ttctgcttcc tttaccaatg tcggcaaatg gtggtacggc
     2581 gcgctaatcg gtgtgatgtg cgtattgatc cgtgtggtca atccggctta ccccgaaggc
     2641 atgatgttgg cgattctgtt tgccaacctg tttgccccga ttttcgacta tttcgtcgca
     2701 caagcgaaca tcaaacgcag aaaggcgcgc agcaatggct aagaaattcg ataaagacag
     2761 cttcagcggc acgctgattg tcgtgttggc ggtcagcctg atttgttcca tcatcgttgc
     2821 tggtgcggtc gtcggtttga aacccatcca agagaaacaa aaactccaag acaaacaagg
     2881 ctatatcttg agcgtagccg gtttgatgga taaaaacacc gacattagca aaacctttgc
     2941 cgagcgtatc gagcaacgtg ttgtcgattt ggcgaccggc gaatatgtcg ctgatgcgcc
     3001 taaagacttc agcgcacgcg ttgccggcaa agaccctgcc caaagcatcc aaatcaaacc
     3061 tgaagacgat ttggcaggca tcaaaagccg tgccaaatac accgaggttt atttggtaaa
     3121 aggcgaagac ggcaaaatcg gccaaatcat cctgccgatg cacggcaacg gtttgtggtc
     3181 ggtcatgtac ggctttgtcg ccatccaacc cgacggcaac accatcaacg gcattaccta
     3241 ctacgagcaa ggcgaaaccc cgggcttggg cggcgaaatc ggcaatcctt tgtggcagca
     3301 aaaattcgtc ggcaaaaaac tgtttgacgg acaaggcaaa ctcgccctgc acgtcggcaa
     3361 aggcgcgggt tcggacaaag aacacggcgt agatgccctc tccggtgcat cgctgacatc
     3421 caaaggcgtg caaggttcgt tcgcctactg gttcggcgaa aacggctata tcccctacct
     3481 gaacaaattg aaatcagcag gagcgcaata atggctgata tgaaacgctt gaaacatttg
     3541 atgttttcac cctttatcaa caacaacccg attgccttgc aggttttggg tatttgttcg
     3601 gctttggcgg ttaccaccaa acttcagacg gccatcgtga tgggtatttc cgtagctttg
     3661 gtaaccggtt tttccagctt cttcatttcg ctggtacgca actacatccc caacagcatc
     3721 cgtattatcg tgcaaatggc gattatcgcg tcgctggtta cgctggtcga ccaactcttg
     3781 caggcatttg cctacgaatt gtccaaacag ctttccgtat tcgtcggtct gattattacc
     3841 aactgtatcg tgatgggccg cgccgaagca tttgcgatga aagagccgcc gctggaaagc
     3901 ctgatcgacg gcatcggcaa cggcgcgggc tacgggataa tgctgcttgt cgtcgccacc
     3961 gtccgcgaac tgattggctc gggcaaactc ttgggctaca ccgttttcca aaccgtgcag
     4021 gacggcggct ggtatcagac caacggcttg ttcctgctcg cccccagcgc gttcttcatc
     4081 atcggctttt tgatttgggg actgcgtacc tggaaacccg aacaggcgga ggaataagct
     4141 atggaacact atttgagcct cttcatcaaa tccgtcttca ttgaaaatat ggcactatcc
     4201 ttctttttgg gtatgtgcac gtttttggcg gtatccaaaa aagtatccac cgcattcggt
     4261 ttgggtgtgg cggtaatttt cgtactcggg ctgtccgtcc ctgtcaacca actcgtttac
     4321 tcgctgctca aagacggcgc gattgtcgaa ggcgtggatt tgaccttttt gaaattcatc
     4381 accttcatcg gcgtgattgc cgctttggtg cagattttgg aaatgttctt ggataaattc
     4441 gtccccgccc tctataacgc attgggcatc tacctgccgc tgattaccgt gaactgcgcg
     4501 attttcggcg ccgtatcgtt tatggcgcaa cgcgaataca acttcggcga atccgttgta
     4561 tacggcttcg gcgcgggctt gggctggatg ttggcgattg tcgctttggc gggcattacc
     4621 gaaaaaatga aatattcgga cgctcccaaa ggcctcaaag gcttgggcat tacctttatc
     4681 gccgccggcc tgatggcgat ggcgtttatg tcgttctccg gcatccagtt ataagaaagg
     4741 gatcggtatg gagattattt taggtatcgt gatgtttacc gtcatcgttt tggttttggc
     4801 actgatgatt ctgtttgcca aatccaaact ggtcagcgaa ggcgacatca ccatcaaagt
     4861 caacggcgaa aaagagctga cgatgcccgc cggcggcaaa ctcttgggcg cgcttgccaa
     4921 cgaaggcatc tttatcccct ccgcctgcgg cgggggcggt tcgtgcggac aatgccgcgt
     4981 cgtcgtaaaa agcggcggcg gcgacatcct gccgaccgag ctgtcccaca tcagcaaacg
     5041 cgaagcacgc gaaggctgcc gtctgtcttg tcaggtcaac gtcaaaaccg acatggacat
     5101 cgaagtcccc gaagaagtgt tcggcgtgaa aaaatgggaa tgcaccgtca tctccaacga
     5161 caacaaagcc acgttcatta aagaactcaa gcttgccatt cctgaaggcg aagaagttcc
     5221 tttccgcgcc ggcggctaca ttcaaattga agccccttct cacactgttg cctacaaaga
     5281 cttcgacatt cctaaggaat accacgaaga ctgggacaaa tacaatctgt ggcaatacgt
     5341 ttccaaagtg gatgagccga ttttgcgcgc ctactctatg gcttcgtatc ctgaagaaaa
     5401 aggcatcatt atgctgaacg tgcgtatcgc cacgccgccc ccgcgcgtac ccgatgcgcc
     5461 tccgggacaa atgtcttcct acatctggtc gctcaaaccc ggcgacaaag ttacgatttc
     5521 cggcccgttc ggcgaatttt tcgccaaaga caccgatgcc gaaatggtat ttatcggcgg
     5581 tggtgcgggt atggctccga tgcgttccca cattttcgac cagttgaaac gtttgaactc
     5641 caaacgcaaa attaccttct ggtatggcgc acgttccaaa cgcgagatgt tctatgtcga
     5701 agactttgac caactcgcag cagaattccc gaacttcacg tggcacgtcg ccctgtccga
     5761 tccactgccg gaagacaact gggacggcta cacaggcttc attcacaacg tggtttacga
     5821 aaaccatctg aaaaaccacg aagcgccgga agactgcgaa ttttatatgt gcggcccgcc
     5881 gattatgaac cagtccgtca tcaaaatgct caaagacttg ggtgtggaag acgaaaacat
     5941 cctcttggac gatttcggcg gttaaatatt cggcttcaaa cacaaaagga agaaacctcg
     6001 gtttcttcct ttttttatcc gataccgtcc gaacaccttt cagacggcat caacataaaa
     6061 acataccggc cgttttgcga tataattcaa gccatttgcc aagactatca aacactatgc
     6121 cgtctgaaac acgcctgccg aactttatcc gcgccttgat atttgccctg ggttttatct
     6181 tcctaaacgc ctgttcggaa caaaccgcgc aaaccgttac cctgcaaggc gaaacgatgg
     6241 gcacgaccta taccgtcaaa tacctttcaa ataatcgggg caaactcccc tcccccgccg
     6301 aaatacaaaa gcgcatcgat gacgcgctta aagaagtcaa ccggcagatg tccacctatc
     6361 agcccgactc tgaaatcagc cggttcaacc aacacacagc cggcaaaccc ctccgcattt
     6421 caagtgactt cgcatacgtt accgccgaag ccgtccgcct gaaccgcctg acgcacggcg
     6481 cactggacgt aaccgtcggc cccttggtca acctttgggg attcggcccc gacaaatccg
     6541 ttacccgtga accgtcgccg gaacaaatca aacaggcggc atcttatacg ggcatagaca
     6601 aaatcatttt gaaacaaggc aaagattacg cttccttgag caaaacccac cccaaggcct
     6661 atttggattt atcttcgatt gccaaaggct tcggcgttga taaagttgcg ggcgaactgg
     6721 aaaaatacgg cattcaaaat tatctggtcg aaatcggcgg cgagttgcac ggcaaaggca
     6781 aaaacgcgcg cggcgaaccg tggcgcatcg gtatcgagca gcccaatatc gtccaaggcg
     6841 gcaatacgca gattatcgtc ccgctgaaca accgttcgct tgccacttcc ggcgattacc
     6901 gtattttcca cgtcgataaa aacggcaaac gcctctccca tatcatcaac ccgaacaaca
     6961 aacgacccat cagccacaac ctcgcctcca tcagcgtggt cgcagacagt gcgatgacgg
     7021 cggacggctt gtccacagga ttattcgtat tgggcgaaac cgaagcctta aagctggcag
     7081 agcgcgaaaa actcgctgtt ttcctgattg tcagggataa aggcggctac cgcaccgcca
     7141 tgtcttccga atttgaaaaa ctgctccgct aaacatacaa accgattagg aaacaccatg
     7201 aaaaccctgc tccttacctt cggcatcttc ctgaccgtca tcatcggtat ggcggtcggc
     7261 tatattttct ccaaacgcag catcaaaggc agttgcggcg gcattactgc cttgggtatg
     7321 aaaaaaatgt gcgactgcga cacaccttgc gacaccctgc aaaagaaact ggatgaagaa
     7381 aaacaggcag gcggcatacg ggttgaccgt taacgccgaa acaaaatgcc gtctgaagcc
     7441 cgacaggcgg gttcagacgg cattgccgct ttacttcgat cggtttatac gcaaaacgcg
     7501 cttcaggaaa taaatcagat tattccccag acgccttcag gcttccttct gtgccaccgt
     7561 aaccatagcc gggcgcaaca cgcggtcgga cagcgtataa cccttcttca tcacacccac
     7621 cacggtattc ggctcctgtt cgcttgccac cgcctgcatc gcctgatgga tattcggatc
     7681 gagcttatcg cccgctttag ggttgatttc cttgatttgc gtagcatcaa atgctttctg
     7741 caactcgttc aaagtcatct gcacgcccat tttcagcgca tcgaaattgc cgctctgatc
     7801 caaaagcgcc atttccagat aatccttgac cggcagcatt tccacggcaa acttctgtcc
     7861 ggcgaacttg tgcgtatccg caatttcctg ctggtggcgg cggcgcaggt tttgctcgtt
     7921 tgccaaagcg cgcagctgct cgtctttcaa ctgcgcttcc agctcggcaa tccgcgcctg
     7981 caaatcctca taagccggct ctgcggcagc ctgttcctgc acaccgtccg catttcctac
     8041 tgtctcgacg gtttccaccg cctccacatt ttcaaccgct tcttcactgt tttgctgctg
     8101 tgtctgttcg ctcatatcgt atcccttaaa acaaattgga aatcaaaatc cagttattac
     8161 ccgcgcaaga taaggacatt ttcaagaaac ttcaagcaaa agcagagaat ttcaaattca
     8221 ttttcaaatc ccctaccgaa aaaataatat agacggtaag atactgccga ccatatcgat
     8281 tttcaaacgt catcactcat gaaaaaagac catttaaaca caaccggttt cgacctctgg
     8341 cacaccatcc gcgaagaaac cgcggccgcg gtctccgccg aaccgatgct ggcaagtttt
     8401 ttgcaccaaa ccgtgttgcg ccacgagtcc ctcggctccg tccttgccta ccacctttcc
     8461 agcaaactcg gcagcccgat tatggacgtg cgcgcgctgt ttgaaatcta ccagcaggcg
     8521 ttgggcagcg acacccaaat cggcaaatgc gtcgaagcag acttaaaagc catctacgaa
     8581 cgcgatcccg cctgcgacga atattcgctg ccgcttttat atttcaaagg cttccacgcg
     8641 attcaggcac accgcatcaa ccatcggctg tatctcgacg ggcgcaaaac gctggcgtat
     8701 ttcctgcaaa accgtatgtc cgaagtattc ggcgtggaca tccatcccgc cgcccgtttc
     8761 ggatacgggc tgatgctcga ccacgccacc ggctttgttg ccggcgaaac cgccgtgttg
     8821 ggcaacaata tttcgatttt gcacggcgta acgctcggcg gttcgggcaa agaaggcggc
     8881 gaccgccacc ctaaaatcgg cgacggcgtg atgatcggcg caaacgcctc gatattgggc
     8941 aatatccgca tcggcagcaa tgccaaaatc ggggcgggca gcgtcgtggt ttcagacgtg
     9001 ccgccgtcca tcacggttgt cggcgtaccc gccaaacccg tggcgcgatc gctcaaaaca
     9061 ccgtcggcgg atatggatca aaatatccag tttaccgaaa tcgactttat gatttgaata
     9121 tccgcaccaa tgccgtctga aacgccaaac gggcttcaga cgacattgcc cgcttcaagc
     9181 ctgcgccttg atatgccgcc gatgtcggca cttgccagtc ggacaaaatg ccttttattc
     9241 aatacacccg attcccacgc aaccggaaat atagtggatt aacaaaaatc aggacaaggc
     9301 gacgaagccg cagacagtac g
//
LOCUS       DAXHMX010000080         9266 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHMX010000080 DAXHMX010000000
VERSION     DAXHMX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.080"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            81..899
                     /gene="opc"
                     /locus_tag="ACFM4S_001356"
                     /gene_synonym="opcA"
     CDS             81..899
                     /gene="opc"
                     /locus_tag="ACFM4S_001356"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC6051019.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLKSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVPTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1062..1706
                     /locus_tag="ACFM4S_001357"
     CDS             1062..1706
                     /locus_tag="ACFM4S_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6051020.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            1725..3635
                     /locus_tag="ACFM4S_001358"
     CDS             1725..3635
                     /locus_tag="ACFM4S_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6051021.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGKIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENETVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            3656..4021
                     /locus_tag="ACFM4S_001359"
     CDS             3656..4021
                     /locus_tag="ACFM4S_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051022.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4132..5607
                     /gene="trpE"
                     /locus_tag="ACFM4S_001360"
     CDS             4132..5607
                     /gene="trpE"
                     /locus_tag="ACFM4S_001360"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6051023.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERNDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            5604..6150
                     /locus_tag="ACFM4S_001361"
                     /pseudo
     CDS             5604..6150
                     /locus_tag="ACFM4S_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
     gene            6147..7283
                     /locus_tag="ACFM4S_001362"
     CDS             6147..7283
                     /locus_tag="ACFM4S_001362"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6051024.1"
                     /translation="MKNISLSPPATLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFNDQTALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7330..7812
                     /locus_tag="ACFM4S_001363"
     CDS             7330..7812
                     /locus_tag="ACFM4S_001363"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6051025.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            7918..8973
                     /locus_tag="ACFM4S_001364"
     CDS             7918..8973
                     /locus_tag="ACFM4S_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6051026.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            8970..9167
                     /locus_tag="ACFM4S_001365"
     CDS             8970..9167
                     /locus_tag="ACFM4S_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051027.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPF"
BASE COUNT         2567 a         2636 c         2057 g         2006 t
ORIGIN      
        1 agaatataat tgtaagcgta acgattattt acgttatgtt accatatccg actacaatcc
       61 aaattttgga gattttaact atgaaaaaaa cagtttttac atgtgccatg attgccctga
      121 ccggtactgc cgccgctgca caagagcttc aaaccgctaa tgagtttacc gtccacaccg
      181 acctctcttc catttcttca actcgtgctt tcctgaaaga aaaacacaaa gctgccaaac
      241 acatcggcgt acgtgctgat attccttttg atgccaacca aggcatccgc ttggaagccg
      301 gtttcgggcg cagcaaaaaa aatattatta atttggaaac agatgagaac aagctgggta
      361 agactaaaaa tgtaaaactg cccaccggcg ttcctgaaaa ccgtatcgat ctttacacag
      421 gctacaccta cacccaaacg ttaagtgatt ctttaaattt ccgtgtgggt gccggcttgg
      481 gttttgaatc ttcaaaagac agcattaaaa ccaccaagca tacgcttcac agcagccgtc
      541 agtcgtggtc agccaaagtt cacgcggatt tgctttccca actgggtaac ggctggtata
      601 tcaacccttg gtctgaagtg aaatttgacc tcaagtcccg ctataaatta aacaccggcg
      661 ttaccagtct caaaaaagac atcaatcaaa aaaccaacgg ctggggcttt ggattgggtg
      721 caaatattgg taaaaaactg ggcgaatccg ccagcatcga ggcggggccg ttctacaaac
      781 aacgtactta caaagaatcc ggcgagttta gtgtaccaac caagagtggc gacgtatcgc
      841 tcaccatccc gaaaaccagt attcgtgaat acggcttgcg cgtcggcata aaattctgat
      901 gatttgaaat catcattgtc gctttaaatg ccaaaccgca aactattttg gtttgcggtt
      961 tttacgtgaa atgaatttga atagccgatg ccgtctgaaa atttcagacg gcatttttcc
     1021 aatcggttaa aatacgcggt ttgttttttt aggaacgcgc cgtgcttgcc tccgccatcg
     1081 acttcatcct ccatatcgac caacacctgc tcgcgctgtc ggcgcaatac ggtgtgtgga
     1141 tttatgcgat tctgtttttg attgtttttt gcgaaaccgg cctgattgtt acgcccttac
     1201 tgcccggcga ttcgctgctg tttgcggccg gcggtattgc cgcgctgggc ggtatggata
     1261 ttcatttgat ggttgcatta ttaagccttg ccgccatact cggcgacgcg ctcaatttta
     1321 ccgtcggcaa atatttcggc ggcaggctgt tcgccaatcc cgattctaaa atcttccgac
     1381 gcgaatatct cgataaaacc caccgttttt acgaaaaaca cggcggcaaa acgattatca
     1441 tcgcgcgctt cgtgcccatc gtccgcacct tcgccccctt tgtcgccggt atggggaaaa
     1501 tgcactatgc caaatttatc cgctacaaca tcatcggtgg cttgttgtgg gttatcctgt
     1561 tttcttacgc gggctatttc tttgccaact tccccgtcgt aaaaaacaat ctgggcttgg
     1621 tgatgggcgg catcattatt gtttccgtcc tgccgggtgc ggttgaaatt gcccgcgcca
     1681 aacttgcggt aaaatccaaa cggtaactgt tgagaatttg tatcatgaat atattatctg
     1741 tagaaaacgc ttcttttgcc atcggccacg ttgccttgct cgacaaaact tcttttcaac
     1801 tcgacagcgg cgaaaaagtc ggcttgatcg gacgcaacgg cgcaggcaag tcttcgtttt
     1861 taaaaatcct cgccggcgtg caaaagctcg acgacgggca gattattgtt caaaacaacc
     1921 tcaaaatcgt ttatgtaccg caggaatcct tttttgataa ggacgcaacc gtatttgata
     1981 ccgttgccga aggtttgggt aaaattcgcg atttattgcg ccgttaccac cgcgtcggtc
     2041 atgagttgga aaacggttcg ggtgaggctt tgttgaaaga actcaacgaa ttacaacttg
     2101 aaatcgaagc gaaggacggc tggaagctgg atgcggcagt caagcagact ttgggcgaac
     2161 tcggtttgcc ggaaaacgaa aaaatcggca acctttccgg cggtcagaaa aagcgtgtcg
     2221 ccttggcgca ggcttgggtg cagaagcccg acgtattgct gctggacgaa ccgaccaacc
     2281 atttggatat tgacgcgatt atctggttgg aaaacctgct caaggcgttt gaaggcagct
     2341 tggtcgtgat tacccacgac cgccgttttt tggataatat cgctacgcgg attgtcgaac
     2401 ttgaccgcgg cattctacgt tcctatcccg gctcgttctc taaatacagt gagaaaaaag
     2461 cgcaagagtt ggcagtcgag gcggaacata accgcttgtt tgacaaattt catgctcagg
     2521 aagaagcgtg gatacgcaaa ggcatcgaag cgcgacgtac ccgtaacgaa ggacgcgtgc
     2581 gccgtttgga agagctgcgc cgccaacgtg cagaacgccg caacgtgcag ggacaggtca
     2641 actttaagct cgatagcggt gagaaaagcg gcaaaatcat cgccgaattg gaacacgctt
     2701 cgtttgccta tggcggcaaa gtcattatgg acaaattctc cgccatcttg cagcgtggcg
     2761 acaaaatcgg cttaatcggc ccaaacggta tcggcaaaac caccttcctc aagctgattt
     2821 tgggcgaatt gcagccgacc tacggcagaa tccgtatcgg cagcaagcag gaagtcgcct
     2881 atttcgacca gttccgcagc gcgttgaatg aaaacgaaac cgtgttttac acgctcggac
     2941 agggcaatga ttacgttgaa gtcggcggta agaaaaagca tgtgatgagc tatttagaag
     3001 atttcctgtt cccccctgcc cgcgcgcaaa gtcctgtttc ttcactctct ggcggcgaac
     3061 gcaaccgcct tttgctggca aaactcttta cccgtcctgc taatattttg attttggacg
     3121 aaccgaccaa cgacttggat atcgataccc aagagctgct tgaagatttg ctgcgcgatt
     3181 atcaaggcac ggtattcctt gtctcacacg accgtatgtt cctcgataac gtgattaccc
     3241 aaagcattat ttttgaagga aatggaaggt taaaagaata tatcggcggc tatcaggact
     3301 acatcgacgc aaaatcacgc gaagcgaaga ttcagacgga atccgcaccc aaaacagccg
     3361 atgccgaacc ggtgaaagaa aaacccaaag ccaaccgcac ggtcaaactg tcctacaaag
     3421 aacagcgcga actcgacgcc ctgcccgacg aaatcgccgc cttggaagcc gaacaggcag
     3481 aaatcaacgc gcagctttcc gatcccgaaa tcttcaaaga ttacgaaaaa gccggcgcac
     3541 tgcaaagccg cgccgaagaa atcgaaatgc tgcttttgga aaagctggaa cgctgggagc
     3601 tgctggaaac caaacagaac ggcaatgccg tctgaaatca aacaggaaga aaaaaatgtt
     3661 caaacgacct gaagaaatca ttgtcctgat acttgccgtc ctctggattg ccggtacgta
     3721 ttttctcgcc gccctgtttg gagcggatgc ttataccgtg ttaaaaatca ctgcccttac
     3781 cctgctttgg tctgccgcat cattcctcct ttggcaaaaa aacctcagcc ggcatatctg
     3841 gccgctgctg ctcggctgcc tgactgcctg ttggtggccg tatctgaatc aatcggtcgc
     3901 acccggttct ttaccttggt atgcatcatg gacgttcaaa accatcttag ccctgattcc
     3961 cgtaaccgca ggttatctgt ttaaatggaa acaataccgg cagcgcaaaa caaagccgta
     4021 agcccgtcct tttcagacgg catctccggt cagactgtca acaattccga taaaatgcta
     4081 tactcgcgcc cgttgccgcg ccgtccgaaa ccgccctttg ggaaagcaga aatgatcagc
     4141 aaacaagaat accaagccca agccgcccaa ggctacaacc gcatcccgct cgtgcaagaa
     4201 ctccttgccg atttggatac gccgctttcc ctctatctca aactcgccaa ccgcccctat
     4261 acctacctgc tcgaatccgt tgtcggtggc gaacgtttcg gccgctattc ttttatcggc
     4321 ctgccttgca gccactatct caaagccagc ggcaaacacg tcgatgttta tcaaaacggc
     4381 gaaattgtcg agcaacacga cggcaacccc ctgcccttta tcgaagcctt ccacaaccgc
     4441 ttcaaaacac ccgaaatccc aagcctgccg cgctttaccg gcggactggt cggctacttc
     4501 ggttacgaaa ccatctacaa ttttgaacac ttcgcccacc gcctgaaaaa caccaccaaa
     4561 gccgacccgc tcggcacgcc cgacatcttg ctgatgctgt cgcaagaatt ggcggtgatc
     4621 gacaatttga gcggcaaaat ccatctcgtc gtttacgccg atccgtcgca gcccgacggc
     4681 tacgaacgcg cccgcgaacg cctcgaagac atccgcaccc agttgcgcca aagctgcgcc
     4741 atcccgctct cgctcggcag caaacacacc gaagccgtca gcgaattcgg cgaagagccg
     4801 ttcaaagcct gcgttaacaa aatcaaagac tacatcttcg caggcgactg catgcaggtt
     4861 gtccccagcc agcgcatgag catggaattc accgacagcc cgctcgccct ctaccgcgcc
     4921 ctgcgtacgc tcaacccttc gccttatctc ttttactacg atttcggcga tttccacatc
     4981 gtcggctcct cgcccgaaat cctcgtccga cgcgaacgca acgacgttat cgtccgcccc
     5041 atcgccggca cgcgcctgcg cggcaaaacc cccgccgaag accttgccaa cgaacaagat
     5101 ttgttaagcg atgccaaaga aatcgccgaa cacgtcatgt tgattgactt aggacgcaac
     5161 gacgtcggcc gcatcagtaa aaccggcgaa gtcaaagtta ccgacaaaat ggtgattgaa
     5221 aaatactccc atgtaatgca catcgtttcc aacgtcgaag gccgtctgaa agacggcatg
     5281 accaacatgg acatcctcgc cgctaccttc cctgccggca cactctccgg cgcacccaaa
     5341 gtccgcgcca tggaaatcat cgaagaagtc gaaccgagca aacgcggcat ctacggcggt
     5401 gccgtcggcg tatggggttt caacaacgac atggatttgg caatcgccat ccgcaccgcc
     5461 gtagtaaaaa acaacacgct atacgtccaa agcggcgcgg gcgtggttgc cgactccgat
     5521 ccggcctccg aatggcagga aacacaaaac aaagcccgag ccgtcataca cgccgcgcaa
     5581 atggtacagg aagggttgga taaatgactt atgaaaaact ttatgagctt cgccaaacac
     5641 tcagaccgac taccaaagac ggtctctata cagataatga aaaaaatcga gaaatcctaa
     5701 cggttagatg gagcggcaac acagaaaatc ctaaaaattt ctcagctgtc gctataggaa
     5761 ttaatccaag taaagcaaat gatgaaagaa gcgacaaaac ccttacccaa cttgcacgtt
     5821 tcttggatat gtacggtttt accaacttca aaatgttaaa tattttttcc agttactcta
     5881 cacagcaaac aggaattagg acaaacaccc aaaccgattt ttccaaattt aaaggatgtt
     5941 tggaagatgc tgatatgatt attcttgcat ggggtacaga ccgcggtgct tataaggatg
     6001 aaaaaaaaca gaatactgga atttctaaaa gcagaaaaat ttatggaaaa agtattctgt
     6061 atttctgaaa ctggtaattc cagcgataca agacatccgt caagaattag ttatagctat
     6121 caacttgtac aatttgaaga gtcagcatga aaaacatatc tctttctccg cccgccacgc
     6181 tcggaatcct cggcggcgga caattaggca gaatgtttac cgttgccgcc aaaaccatgg
     6241 gctacaaagt aaccgtactc gaccccgatc cgaacgctcc ggcggcggaa tttgccgacc
     6301 gccatttgtg cgcgccgttt aacgaccaga ccgcgttgga cgagttggca aaatgcgcgg
     6361 cggttacgac cgaatttgaa aacgtcaatg ccgacgcgat gcgttttctg gcaaaacata
     6421 ccaacgtttc ccccagcggc gactgcgttg ccatcgcgca aaaccgcatt caggaaaagg
     6481 catggatacg caaagcaggt ctgcaaaccg cgccgtatca agcagtttgc aaagccgaag
     6541 acatcactga agcaagcgcg caattcctgc ccggtattct gaaaacggcc actttgggct
     6601 acgacggcaa aggtcaaatc cgcgtcaaaa cggtggacga actcaaagcc gcgtttgccg
     6661 aacacggcgg cgtggattgc gttttggaaa aaatggtgga tttgcgcggc gaaatttccg
     6721 ttatcgtatg ccgtctgaac aatgacaacg tgcaaacttt cgatcctgcc gaaaacattc
     6781 acgaaaacgg tatcctcgcc tactccatcg tcccagcccg actgagtgcc gacattcagc
     6841 aacaggcgcg acaaatggcg cagcgtttgg ccgatgaatt gaactacgtc ggcgtattgg
     6901 cggtagaaat gtttgttgtc ggcgacacgc atgaattggt cgtcaacgaa atcgcgccgc
     6961 gtccgcacaa ttccggccac cataccgtcg acgcctgcgc ggcagaccaa ttccagcaac
     7021 aggtccgcct gatgtgcaac ctgccacctg ctgacaccaa attgctgagt tcttgctgta
     7081 tggcgaatat tttgggcgac gtttggcagg aagacggcgg cgaaccggat tggtttcccc
     7141 tgcaaagccg gccggacgcg cacctgcacc tttacggcaa aaaaaccgcg cacaaagggc
     7201 ggaaaatggg acactttacc gttttaacca ccgattcgga caccgcattt caagaagcaa
     7261 aaaaactgca tcagtcccta taatccgacc aacggcacag atgccgtctg aagccgccga
     7321 catactgcca tgaacctgct gacactgtta cgccccgcca ccgtgcaaga ctgcaaagac
     7381 atcttcaaag tccacctgca ctccgtccaa tacacctgca tcctcagcta caacgaacac
     7441 gccctaaaag tatgggaagg cttgttaaac acagaaagct acctgccgac catttccgat
     7501 cccgacaaag cactttgggt tgccgaatac aaaggcaaca tacagggatt tttccaaatc
     7561 gactgccaag aagcacaact cgatgcactt tacgtccacc ccctgttcca caacctcgga
     7621 ctgggcactg ccctgcttca tcaggcggaa accatcgccc acaaatctgg tttgagcttt
     7681 ctgaaactct acgcatcgct caactccgtc ccgttttacc ttctcaaccg ctacgaatcg
     7741 ctcggttcgg cagtattgca gcttgacccc agcatcaaaa tcaaatgcga actgatgcgg
     7801 aaacacctct gatccgtcct tataaccaaa actgataatc ttcaaatcaa acattatttc
     7861 cacaagcgca gacagccgta taaagttgcc ccatctacca accgacagga caagcatatg
     7921 aaaacctacg caccggcact ctataccgca gccctgctca ccgcctgcag ccccgcagcc
     7981 gattcaaacc atccgtccgg acaaaatgcc ccggccaata ccgaatccga cggaaaaaac
     8041 attaccctgc tcaacgcctc atacgatgtg gcacgggatt tttacaaaga atacaacccc
     8101 ttatttatca aaacatacca atccgaacac cccggcacat ccgtcagcat ccaacagtcc
     8161 cacggcggct ccagcaaaca ggcattatcc gtagccaacg gccttcaagc cgatgtcgta
     8221 accatgaacc aatcctccga catcgacctg ctcgaaaaaa aaggactggt agaaaaaggc
     8281 tggcaacaag ccctccccga ccacgccgcg ccctacacca gcactatggt tttccttgtc
     8341 cgaaaaaaca accccaaaca gatccgcgat tggaacgacc ttgccaaaga cggcgttaac
     8401 atcgtcatcg ccaatcccaa aacctcgggc aacggacgct acgccttcct cggcgcatac
     8461 ggttacggtc tgaaagccac caacggcaac gaacaggaag cccaaaaact cgtcgcatcc
     8521 atcctcaaaa acacccccgt ttttgaaaac ggcggacgcg ccgccaccac caccttcaca
     8581 caacgcaaca tcggcgacgt actcattact tttgaaaacg aagccaacta cgtcagcaaa
     8641 aaactgaccc aaggacagtt tgaaatcgtc tatccgagct acaccatttc cgccgaaagc
     8701 cccgtcgccg tcgtcaacag cgtcgtcgcg aaaaaaggca cgcaaaaaac cgcccgcgcc
     8761 tatctcgaat acctttggag cgaaccggcg caagaactcg ccgcctcact ctacctgcgc
     8821 ccccgcaacc ccgaagtatt ggcaagacac aaagccgact tcccagactt agacaccttc
     8881 tctcccgaag aaaaattcgg cggatgggac aacatcatga aaacctactt cgctgacggc
     8941 ggcatattcg accgcctgac cgcacaaaaa tgaccgctat gccgtctgaa ccgcctttca
     9001 gacggcattc cacacatcaa aacagaaaaa ccgcgcccaa aatcatcatc acaagacaca
     9061 acggcaaacc ggatacaaaa cccaacaccg ccgccaaaat gccgtctgaa gccgtcggcg
     9121 caaccatcct tatctggggc ataactttcc ttaaccatcc cttttgacag ccaaataaac
     9181 agtgcttttg cctgtcagaa acaaaaccaa tattttgcag ttcgtatcgg aacatttata
     9241 gtggattaac aaaaaccagt acagcg
//
LOCUS       DAXHMX010000081         9214 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHMX010000081 DAXHMX010000000
VERSION     DAXHMX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.081"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(105..299)
                     /locus_tag="ACFM4S_001366"
     CDS             complement(105..299)
                     /locus_tag="ACFM4S_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6051028.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(289..2370)
                     /locus_tag="ACFM4S_001367"
     CDS             complement(289..2370)
                     /locus_tag="ACFM4S_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6051029.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNHLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2847..3227
                     /locus_tag="ACFM4S_001368"
     CDS             2847..3227
                     /locus_tag="ACFM4S_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6051030.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3360..3854)
                     /locus_tag="ACFM4S_001369"
     CDS             complement(3360..3854)
                     /locus_tag="ACFM4S_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HFC6051031.1"
                     /translation="MIFKQNQNYWTVFYTNEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTRR
                     ALFKNMMSVDIWLHNGCLKISPSRHVKLEAWDAIDADDVILSLDNSPEEIGAGLKLAL
                     SRCR"
     gene            3991..4155
                     /locus_tag="ACFM4S_001370"
     CDS             3991..4155
                     /locus_tag="ACFM4S_001370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051032.1"
                     /translation="MYKKVFFLNDRDCARAIFRHRPDVALPDRMLQTASEGFAVLEYE
                     DDNVREIDGI"
     gene            4188..5621
                     /locus_tag="ACFM4S_001371"
     CDS             4188..5621
                     /locus_tag="ACFM4S_001371"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6051033.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGIPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            5730..6518
                     /locus_tag="ACFM4S_001372"
     CDS             5730..6518
                     /locus_tag="ACFM4S_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6051034.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            6886..8442
                     /locus_tag="ACFM4S_001373"
     CDS             6886..8442
                     /locus_tag="ACFM4S_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6051035.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8459..9205
                     /gene="surE"
                     /locus_tag="ACFM4S_001374"
     CDS             8459..9205
                     /gene="surE"
                     /locus_tag="ACFM4S_001374"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6051036.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKTPPQFPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
BASE COUNT         2157 a         2316 c         2613 g         2128 t
ORIGIN      
        1 gtactgtctg cggcttcgcc gccttgtcct gatttttgtt aatccactat attataaaaa
       61 ttccgtctga agtggcgttc agacggaatt tgtttcaagc aggcttaaca gcagcgtccg
      121 ccgtttgcgc cgcaacggcg cttgcggcag tagtcttgaa attgcagctt ggtcatcacg
      181 ggggcgttgg gattatgttt gcgctgctgt gcaacgtagt tttcataatc aggcacgcct
      241 gccatcaagt ttgccgtcag cttgatggtt ttccaccaag acgcgagctt atgcttcact
      301 ttgtgcctcc ggctgcttgc cgtcgcggta caccgccggg atttctttgg cggtcggcca
      361 gccgactttg cgtgctttga gggcggtacg caaaccgtac gcggcgacaa tcacgacaac
      421 cgacaagaag aggatggtca gaccggcatt aatcttgtcg ttgaagatga tttgcgccat
      481 ttcgccgatg tctttggcag gcgcaaggat ttcgttttta gccaatgcgt cgctgtattt
      541 gccggcgtgg gcaaggaagc tgatgcgcgg gtcgctgtgg aacagttttt gcaggccggc
      601 gtagcaggtt acgaacagta cgccgacggc gggaacgagt accacccaga cataacggtc
      661 gcgtttcatc ttaatcagca ccacggcgca cataatcaag gctacgcctg ccagcatttg
      721 gttggcgatg ccgaacaaag gccagagcga gttgatgccg cccaacgggt cggtcacgcc
      781 cgtgtagagg aagtagcccc acaatgccac ggcgaagaag gtcgcaatca ggttggcggg
      841 gatggagtcg gtgttgccga aaggtttgta gaagatgctg cccaagtctt gaatcataaa
      901 acgtgcgacg cgcgtaccgg catcgacggc ggtcaggatg aacaaggctt caaacaatag
      961 ggcgaagtga taccagaacg ccatcatcgc ctcgcccgga atcaggcggc tcataatgtg
     1021 cgccataccg actgcgaggg tgggcgcacc gcctgcacgg gaaaggatgg tgttttcgcc
     1081 cacttcctta gcggtgtgca acagggttgc cgcatcgaca gggaattgca gcttggtggt
     1141 aatcacttcg gcggcggtat tggcatccgt accgatcagg gcggcagggc tgttcatggc
     1201 gaagtacacg ccgggatcaa gcgatgcggc ggcggcaagc gccataatgg ctacgaaact
     1261 ttccatcaac ataccgccgt aaccaatcat acggacgtgg gtttcgtttt ccagcatttt
     1321 cggcgtggtg ccggaggaaa tcagcgcgtg gaagcccgaa accgcaccgc aggcgatggt
     1381 gatgaagagg aacgggaaca atgcgcctga gaataccgga cccgaaccgt cgataaagtg
     1441 ggttacggca ggcatttgca aagcgggatt gacgatgacg atgcccaaag ccaaggccgc
     1501 aatcgtaccg attttcagga aggtggagag atagtcgcgc ggagtgagca gcaaccatac
     1561 gggcaatacg gaggcgacaa agccgtaaat cataatcgcc caagtgagct ggatgccgtc
     1621 aaggtcgaac caatgtccga tggaactttt cgccacatct tcgccgtaaa ttaccgccag
     1681 catcagcaaa ataaagccga cgatggaaat ctcgccgatt ttgcccggac ggatatagcg
     1741 cgtgtaaata cccataaaca gggcaatcgg catagtggcg gcgatggtga acgtacccca
     1801 agggctgtga accagtgctt ttacgacaat caacgccaac accgccataa tgatgaccat
     1861 aatcatcaaa ataccgatgg aggcaatcac gccggggaca gtgccgagtt cctgtttcac
     1921 aatatcgccc aaagacttac catcgcggcg catagagacg aacaagacca tcatatcctg
     1981 taccgcgccg gcaaatacca cgccgaagat aatccacaaa gtaccgggca ggtaacccat
     2041 ttgcgccgcc aaaaccgggc cgaccaaggg gcccgcgccc gcaatcgcgg caaagtggtg
     2101 tccgaacaat acgcccttgt gcgtcggaac gtagtccaag ccgtcgttgt ggcgttctgc
     2161 cggagtcagg tggttaggat cgagccgcat cacgcggttg gcgatgtaga ggctgtaaaa
     2221 acggtaggcg atgcagtaaa cggatatggc ggcggtgacc atccataccg cgctgacctg
     2281 ttcgcctcgg ctgagggcca gagtggtaaa ggatgctaag ccgaccagta ccactatgcc
     2341 ccaaatgagg aaggttttga gtgatttcat cgaataaatc cttatctcac actgtcggaa
     2401 tatgcctgaa cggcgggaaa cggccgaacc atctgccggc acgggcacaa accggagtct
     2461 gaatgattga cgaagtatga aacagtgcgc ttgtcgggat ttgtgcctta tgcccgcatc
     2521 aaacagtgca gggatgcggg cgcacaaaac gttaaacgcc gaatatgatt ttaacgcaaa
     2581 ttagcacact gatagcggtt tttacttgga atttggaaaa aatttacatt cctccgtgcg
     2641 gacagggagg ttcagacggc atcgtcaggc aaaaggcggc gccggaagac gggtaaagaa
     2701 ggggcgcgca atccggatta ttgattcatc gcgggtaaat tctggttatc gggcttgtgt
     2761 gtttgcgcgt ccgtttatag tatggcgttg ccgcagcttg gaatcagggc ggttgtttca
     2821 tatcttattt ttattgggga gcttttatga atatcaggtt tttcgcgctg accgtaccgg
     2881 ttttgtcttt ggcggcctgt gccgtgccgg aggcgtatga tgacggcgga cgagggcata
     2941 tgccgcctgt tcaaaaccaa gccggcacgg acgattttcg ggcgttttcc tgcgagaacg
     3001 gtttgtctgt gcgggtccgc catttggaca gcggcaaagt cgcgttgcgg ctagacggca
     3061 ggcgtgccgt cctctcttcc gacgttgccg catccggaga acgctatacc gccgaacacg
     3121 gtttgttcgg aaacgcaacc gagtggcacc agaaaggcgg cgaagccttt ttcggcttta
     3181 ccgatgccta cggcaattcg gtcgaaactt cctgccgcgc ccgttaaacg gggcagcctg
     3241 tttgaaccgc cgtccggatt tttcgggcgg ttttttgtac cggttttgtt ttggccgtcg
     3301 gctggttccg gcggttttcg ggcgtggcgg attatttttc gttttgttcc gggtttattc
     3361 tatcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3421 aatgataaaa tgacatcgtc tgcatcaatg gcatcccacg cttccagctt gacatggcgg
     3481 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccacgctcat catgttttta
     3541 aatagggcgc gtctggtttt atagcccaag ttcccgcata gcttggcaac ccaatcctca
     3601 tagcgttgcc gaattttttc ggtatcaaaa aaatcttggt cttctggact gtcataaacg
     3661 aaagtcctgc tgtttgccaa tgcttgcaag accgttgtgc ctaaagtttc attgtcggta
     3721 tccaatggca ggatatgagg gggatatagg tggtctattg ccgttaatcc caaacctgaa
     3781 catgtttgaa caatcagagc ttcttcattc gtataaaaaa ctgtccaata attttgattc
     3841 tgtttaaaaa tcattatttt atctccgtaa ttttgactgt aatgttttga cttttgccat
     3901 actctaccac acgttgcaac tgcaatcttt gctccttata tattgcaccc catcaaaggg
     3961 ctgcattact tttctttcta aagattacca ttgtataaaa aagtattttt cttaaacgac
     4021 agggattgcg cccgcgccat attcagacac cgcccagatg ttgcgctgcc cgatcggatg
     4081 cttcagacgg catcggaagg gtttgcagtt ttggaatatg aagatgataa tgtccgcgag
     4141 attgacggca tttgaaagcg gttattccgc ataccggagg acacgaaatg aacgaatatt
     4201 accaattaat caagcatccc gatatttccc tttccccgat ttcagacggc atcggggtcg
     4261 gcaatccggc aacgggcgaa attttggcgt atgtccgcaa tacgggttcg gacaaactga
     4321 aaaacctgat tcaaaaggcg gcggcagcgc aaaaattatg ggcggcgaaa actgctttgg
     4381 aacgcgccga catattgtgg cgttggtatt ttttagttaa agaaaacaaa gaagcactgg
     4441 cgcgcctgat gacgatggag cagggcaaaa gcctgaccga gtcgcgcggc gaaattgatt
     4501 atgcggcttc gtttgtgcgc tggtttgccg aagaggcgcg gcggattgac ggcgatgtgt
     4561 taacgagtgt aaaagcgtcg caaaaactgg ttgtgttgaa acagcctgtc ggcgttaccg
     4621 ccgcgattac gccgtggaac ttcccatctg caatgattgc gcgcaaggct gcgcctgctt
     4681 tggcggtggg ttgcgcgatg atcatcaaac ccgcatcgct cacgcctttg agtgcgtatg
     4741 ccttggcttc gttggcttac gaagcgggca taccgcagga tttgttgccc gtcgtcagcg
     4801 gccgcgcatc ggaaatcggc cgtgaatttg ccacgaaccc gattatccgc aaaatcagct
     4861 tcaccggttc gaccgaagtc ggcgcgaaaa tttttgccga cagcgcggcg gacattaaaa
     4921 aactcagttt ggaactgggc ggcaacgcgc cgtttatcgt gtttgacgat gccgatttgg
     4981 acaaagccgt cgaaggtgcg ctcgccagca agttccgcaa cagcggtcag acctgcgtct
     5041 gcaccaaccg cgtttacgcc caatccggca tttacgacga attttgtcgc aaattgagtg
     5101 aaaaagtagc cgagctcaaa ttgggcaacg gcttggagga tggcgtcaac caagggccgc
     5161 tgattgagga aaaagcggtg gagaaagtcg agcagcacat cgccgacgcg cttgctaaag
     5221 gtgcaagctg cctgaccggc ggcaaacgca gcgcgttggg cggaacgttt tttgagccga
     5281 ccgtcttaag cggcgtaacg gcgcaaatgg cggtggctcg cgaagaaacc ttcgggcctt
     5341 tgtgtccggt attccgtttt gaaaccgaag ccgaagtcat tgaggctgcg aacaatacgg
     5401 aatacggttt ggctgcttac ctttttaccg ctgacaccgc acgccagtgg cgcgtcggcg
     5461 aagcattgga atacggtatg gtcggcatca atacgggctt aatcagtaat gaagcggcac
     5521 cgttcggcgg cgtgaaacgc agcggtttgg gacgtgaagg cagcaaatac ggtgcggacg
     5581 aatatctgga attgaaatat ctgtgtatgg atgtcgggtg acggatgccg tctgaacggc
     5641 agggtttcag acggcgccgc attttaagca gtctctatct gttgtacaat gcgccctgtt
     5701 tttacggtta tttttgattt gaacaagata tgatggaaaa cggaaaaaca gtcccgagat
     5761 ggcgttttgc cttgaaaagt gcgggctggc atctcttaat cagcctgtcg gttgcagggc
     5821 tggcggcatt gctggttttt aaggtttggt atccttatcc ttatgccgag ctgacgggag
     5881 ggctgtcgct ttatcagctg gtggtggctg tcgatattgt atgtggtccg ctgctgactt
     5941 taattttggc aagcccgaag aaaaagacaa aggcacgcat ggtcgatttt tccatggtcg
     6001 gcatcatcca gctggcggct ttggtgtacg gtctgcacag cgtttctttg gcgcgccctg
     6061 tgatagaggc gtttgaaaaa gacaggatta atgttgtgac cgccgccgaa gtggcggttg
     6121 aagatttgca caaagcgccg gaagggctgc aaagcctgtc gtggttcggc atccgccgca
     6181 ttgcattgaa agaacctgag gatgcggatg agaagaacaa gacgctggat ttgtccctga
     6241 aaggtatcga gccgagtatg cgtcccgacc ggtggctgcc gtattccgac aaggaagcag
     6301 aaaaaatccg caaacatctg aaaccgctga aagtcttggc ggatgcgaga aaaacgacgg
     6361 ttgcggacat tctgaaacag gcaggtctcg ccgaagggga ggagctgtat tacctgccgt
     6421 ttaccagcag caggcagaaa gagtggatag tcattaccga taaagagggc aacaccaaag
     6481 gctacgcgcc gatagacggc ttcatcatca ccccttaagc gttgggactc cgtccgcact
     6541 cgaacatccg ttcttcgcgg cggtagaatc agactgtatt tgagaggggc gcaacccctc
     6601 aatcagggca acacataggg gaaggcttta tgtgccgtcc gtgtgttgaa acatttcaaa
     6661 ataagctcat gccgtccgaa catcctttca gacggtatgg cattttgcca ttgccccgat
     6721 aagctgttaa actatttaaa ttatttcgac cgaaggtaca cgcacatgaa agaccccgag
     6781 cagagcagca agcccgcccg ccgttttttg tgcgtaccgt ctgttcaggc agcttgagcg
     6841 cttcaggctg ccttttcgcg tttgaacttg aggaatacga aaatcatgga tttcagttgg
     6901 ttggcagaac cgcatacctg gataggtttt gccacgcttt tggtgttgga agtcgtattg
     6961 gggatagaca accttgtctt tgtggcgatt ttggcaaaca aggtccagcc cgcacggcgc
     7021 gaccgcgcac ggattatcgg gctggggctg gcagtcgtca tccgcatcat tatgcttgct
     7081 tttatggcgc acatcatcac gctgaccgag ccgctgttcc aaatcggcgg cctcgccgtt
     7141 tccggcaagg acatgattat gctcgcgggc ggtattttcc tgctttacaa agccaccacc
     7201 gaactgcatg aacgcctcga agggcacaac cgttttactg ttgccgacaa ccaaaaaaaa
     7261 cacgcgccgt tttggggcgt ggtcgcgcaa atcctgatac tggatgccgt gttttccatc
     7321 gattcggtca ttactgcggt ggcgatggtc gatcatatcg tcgtggcgat gggtgcggtc
     7381 gtcgtcgcaa tggctgtaat gatttctgcc agcaaactct tgaccgaatt tgtcgacaga
     7441 caccctaccg tcgtgatgct ctgccttggt tttttgttga tgatcggttt cagcctgatt
     7501 gccgaagcct tccatttcca cattcccaaa ggctacctct acgccgccat cggcttctcg
     7561 attttaatcg aactgtttaa ccagatttcc cagcgcaaca gccgcaaaaa cgactacatc
     7621 ggcagctcgt ggcgcaaacg gactgctgaa aacgttttgg gtatgatggg catacgcgaa
     7681 agcgtgcttg ccgacgcggg cggcgaatcc ggggacgacg cgcattttga agaaaacgaa
     7741 aaatcgatga tacgcagcgt gctgacgctt gccgaacgcc cgattatggg ggtgatgatc
     7801 ccacgccgcg acatcgaacg gctggacatt tcccaaagcc gcgaagaaca gtgtgcccaa
     7861 ctgcaaaaca cgccttacag ccgcctgctc gttgtcggaa aggcgggcgt ggacgaacct
     7921 ttgggctaca tcaacaaaaa agacctgctg tcccaactgc tggaaacagg cggtctcgac
     7981 attcagacgg cattgcgcca gccgctcgtc ctacccgacg gcacgaccgc cttgggcgcg
     8041 ctcgaactct tccgccaaag cagcgcggat tatgctttgg tggtggacga gttcggcgcg
     8101 gtattgggca tggtaaccat gaaagacctg ctcgaaacca tcgcaggcga gttccccgaa
     8161 gaatttgagc gcgaagaaga accagccgtt caggggaatc ccgatgaaag cctgacggtg
     8221 gaaggcgcgt tggaatatgt ggagctggca tcccaactca atctgccgca gcaggaggaa
     8281 gatgccgatt tccatacggt tgccgggctg attatggaag aattgcaaac catccccgat
     8341 gtcggcgatt ttgccgattt ccacggctgg cggtttgaag tggtcgaaaa agaagggcag
     8401 cgcatcgagc gggtcaaaat caccaaattg cccgaagaat aagcattcag gatagaaaat
     8461 gaacgttttg atttccaacg acgacggcta cctctccgaa ggtattgccg ttttggcgcg
     8521 cgttacggcg gaatttgcca acgtcagggt ggttgcgccc gaacgcgaca ggagcggggt
     8581 cagcaattcg ctgacgctgg aacgcccttt gcagttgaaa caggcgcaaa acgggttcta
     8641 ctatgtcaac ggcacgccga ccgactgcat ccacatcggg cagtctgtat tttcggattt
     8701 tcaggccgat tttgtctttt cgggcatcaa ccggggcgcg aatatggggg acgacacgct
     8761 ttattcgggg acggttgcgg cggcaaccga agcctacctt atgggcatac ccgctgtggc
     8821 gttttcctta aacgacgctt ccggacgcta ttgggcaacc gcagaaaagg cactgtggac
     8881 attgttggcg cattttttca aaaccccccc gcagttccct attttgtgga acatcaatat
     8941 ccccgccgtc gcgccggaag atgtgcgggg cattaaaatc gcccgtttgg gcaggaggca
     9001 tcacggtcag aacgtcatcc ccgcgcgcaa tccgcgcggc gaacagattt attggatagg
     9061 cccggtaggc agggtttccg acaaagaaga gggaacggat ttcggtgaat gcggcgcagg
     9121 tttcattacc gtaacgccgc tgcaaatcga cctgaccgcc tatccggaca tggcggaaac
     9181 agcggtgttc tggcatgcgg actgaccgtt tcat
//
LOCUS       DAXHMX010000082         9206 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHMX010000082 DAXHMX010000000
VERSION     DAXHMX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.082"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            90..299
                     /locus_tag="ACFM4S_001375"
     CDS             90..299
                     /locus_tag="ACFM4S_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6051037.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            388..1602
                     /locus_tag="ACFM4S_001376"
     CDS             388..1602
                     /locus_tag="ACFM4S_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFC6051038.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1689..4268)
                     /gene="clpB"
                     /locus_tag="ACFM4S_001377"
     CDS             complement(1689..4268)
                     /gene="clpB"
                     /locus_tag="ACFM4S_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6051039.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDIMPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4529..5539
                     /gene="trpS"
                     /locus_tag="ACFM4S_001378"
     CDS             4529..5539
                     /gene="trpS"
                     /locus_tag="ACFM4S_001378"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6051040.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5545..5886
                     /locus_tag="ACFM4S_001379"
     CDS             5545..5886
                     /locus_tag="ACFM4S_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051041.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVVGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6208..6531
                     /locus_tag="ACFM4S_001380"
     CDS             6208..6531
                     /locus_tag="ACFM4S_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051042.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6740..8248)
                     /gene="ppx"
                     /locus_tag="ACFM4S_001381"
     CDS             complement(6740..8248)
                     /gene="ppx"
                     /locus_tag="ACFM4S_001381"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6051043.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8364..9206)
                     /locus_tag="ACFM4S_001382"
                     /pseudo
     CDS             complement(8364..9206)
                     /locus_tag="ACFM4S_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714481.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
BASE COUNT         2111 a         2397 c         2437 g         2261 t
ORIGIN      
        1 cgatttgaat ttttcagaaa atgaagcgga cgtttgggcg gaggcaactt gtttgataag
       61 atagcaatat tttaaaacgg agaaagatca tgccttatgt caatattaaa gtaaccggcg
      121 gcaaggaagc accgactgcc gcgcaaaaag cggaactgat cggcggcgta accgaattgc
      181 tggcacgcgt gctgggcaaa aatcccgaaa caacggttgt cgtgattgac gaagtggata
      241 ccgataactg gggaataggc ggcaaaagcg tcagcgaacg gcgcaaagag ggcaggtaaa
      301 aagcctgaaa atctcggttt gatgctttaa attccgcgtg aaaaagagta cattcccacc
      361 cattgcccaa aatttacgga acacatcatg gataaatttc ccaagtctgc aaagctcgat
      421 cacgtctgtt acgacatacg cgggccggtt cacaaaaaag cccttcagtt ggaagaggag
      481 ggcaataaaa tccttaaact caatatcggc aaccctgcgc cgttcggctt tgaagcccct
      541 gatgaaatct tggtcgatgt catccgcaac ctgccgactt cgcaaggcta ttgcgattct
      601 aaagggctgt attccgcccg caaagccatt gttcactact atcagaccaa aggtttgcgc
      661 gatattacgg taaacgatgt ctatatcggc aacggcgtgt ccgagctgat tacgatgtct
      721 atgcaggcat tgctcaacga cggcgacgaa atcctgattc ccgcgcccga ctaccctttg
      781 tggacggcgg cggcaacgct tgcgggcggt acggtacgcc attatctgtg cgacgaagaa
      841 aacggctggt tccccaacct tgccgatatg gaagccaaaa tcacgcccaa aaccaaagcc
      901 atcgtcgtca tcaatcccaa taatccgaca ggcgcggtgt acagcaggga aatcctgttg
      961 gaaatcgccg aactggcgcg caagcacggt ttgattattt tcgccgacga gatttacgat
     1021 aaaatccttt atgacggcgc ggttcaccac cacatcgccg cgcttgcccc cgaccttttg
     1081 acggtaacgt tcaacggttt gtccaaagcc taccgcgtcg ccggcttccg ccaaggctgg
     1141 atggtgctca acgggccgaa acatcatgca aaaggttaca tcgagggttt ggatatgctc
     1201 tcgtctatgc gcctgtgtgc caatacgccg atgcagcacg cgattcagac ggcattgggc
     1261 ggctatcaga gcatcaacga attcattttg cccggcggac ggcttttgga acagcgtaac
     1321 agggcgtggg aattggtcaa tcagataccc ggcgtatcct gcgtcaaacc gatgggcgcg
     1381 atgtatatgt tcccaaaaat cgataccgaa atgtaccgta tccacgatga catgaaattc
     1441 gtttacgatt tgctggtgcg cgaaaaagtc ttgctggtac agggaacggg gtttaattgg
     1501 attaaacctg accatttccg cattgtcacg ctgccttacg tccatcagat tgaagaggcg
     1561 atgggcaggt tggcaagatt cctgcaaacc taccgccaat aaggggacgg tttgtctgcc
     1621 gaggataaaa aatgccgtct gaaacggaga ttcccgtttc agacggcatt ttcaacagca
     1681 ggaacgaatc aggcaaattt cagtctgtcg ccgtcggctt ctaccctgat ttcgctttcg
     1741 ggcgcatagt ttccggcaag cagggctttt gccagcgggt tttcgatttc cgactggatg
     1801 gcgcgtttga gcggacgtgc gccgtaaatc gggtcgaaac cggctttggc gatgatgtcc
     1861 agagcggcat cggaaacagt caggcggagg ttttgttttt ccaaacgttt ttccaagcct
     1921 ttgagctgga ttttcgcaat gttgcggata ttatcctgat ccagtccgtg gaacacgacc
     1981 acttcgtcga tgcggttgat catttcagga cggaaatgct ctttcacatc ctccatcaca
     2041 acttctttca cggcttcgta gtcctgcgtg cccatttgtt ggatgtgctg gctgccaatg
     2101 ttggaagtca tcacaatcac ggtgttttta aaatctaccg tcctgccttg tccgtcggtc
     2161 aaacgaccgt catccaaaac ttgcagcagg atattgaaca cgtcgggatg ggctttttcc
     2221 acttcgtcca aaagaatcac gctgtacggt ttgcggcgga cttgctcagt caggtagccg
     2281 ccttcttcgt agccgacata gcccggaggc gcgccgatta agcgggcaac ggcgtgtttt
     2341 tccatatatt cggacatatc gatacgaatc agatgatctt cactgtcaaa caggaagcct
     2401 gccaatgcct tacacaattc ggtcttaccc acgccggtcg ggcctaagaa caggaagctg
     2461 ccgtaaggtt tgttcggatc ggcaagaccg gaacggctgc ggcggatagc gtcggacacg
     2521 gcacgcacgg cttcatcttg accgaccacg cggcggtgca atacttcttc catcttcagc
     2581 agtttgtcgc gttcgccttc catcattttg gacacgggaa taccggtcat acgggaaaca
     2641 acttctgcga tttcctccgc gccgacatta ttacgcaaga gtttgtttgc cggttttgtg
     2701 ctgtccgtat ctgcccgttc ggcggctgca cgctgttttt ccaaatgctc caaatcttca
     2761 tacatcaatt ttgaagccag tgccaaatcg ccttgccgtt ttgcctgttc gattttaatt
     2821 ttgacttcgt caatttgttt cttaatatta gcagcaccgt ctgaaattgc tttttcggct
     2881 ttccagattt cgtctaaatc ggcgtattct ttttgcagac cgttgatttc ctcgtctatc
     2941 agttccaaac gttttttgct ggcatcgtct ttttcttttt caacgtgtgc cttttccatc
     3001 cgaagctgaa tcagacggcg gtcgattttg tccattgctt ccggcttggt ttctttttcc
     3061 atcttgacac ggctggcggc ttcatcaatc aaatcaatcg ctttgtcggg caggaagcgg
     3121 tcggtgatgt agcggtcgct caactccgct gcggcaacga tagcagggtc ggtaatatcg
     3181 ataccgtggt ggatttcata acgctcctgc aaaccgcgca agatggcgat ggtgtcttcc
     3241 acgctgggct cgccgaccaa tactttttgg aagcggcgtt cgagtgccgc gtctttttcg
     3301 atgtattggc ggtattcgtc caaggtggtc gcgccgatac agtgcagttc gccccgcgcc
     3361 aaggccggtt tcagcatatt gcccgcgtcc atcgcgccgt cggttttgcc cgcgccgacc
     3421 aaagtatgga tttcatcaat gaaaatcaga gtgttgccgt cgtctttcgc caaatcgttc
     3481 aacacgcctt tcaagcgttc ttcaaattcg ccacggtatt tcgcgccggc aatcaatgcc
     3541 gccaagtcca gaaccaaaag gcgtttgttg cgcagggatt cgggtacttc gccgttgacg
     3601 atgcgttgcg ccaagccttc gacaatggca gttttaccca cgcccggttc gccaatcagc
     3661 acggggttgt ttttggtgcg gcgttgcaat acctgaattg cacggcggat ttcgtcgtca
     3721 cgaccgataa cggggtcgag cttaccgtcg cgggcgcgtt gggtcaaatc gagcgtatat
     3781 tttttcagcg catcgcgttg gtcttcggca ttggcatcgt tcacgttttg tcctcctcgt
     3841 accgcatcga ttgcggcatt aatgttttgt tcggtcgcgc cggcttcttt caaaattttg
     3901 ccggttgcat cgttctgctg caccaaggcg agcaggaaaa gttcgctggc gatataggca
     3961 tcgccgcgtt tggttgctgc tttgtccatc aggttcaaca ccgcctgcaa ttcgcggctg
     4021 ggcataatgt cgccgccctg tccggatact ttcggcaggc tgttcaaatg ctgctgcaaa
     4081 cgctgtttca cctgcggcac gttcacgccc gcatgagcca agagcgcggc agcaccgctg
     4141 ttttggtcgt caagcagggc tttcagcaca aagcccgctt ccagatagct gccgtccgca
     4201 gccaacgcca aactctgagc ttctgcaagg gcctgttgga atttggcggt taatttgtcg
     4261 tatcgcattt ttgtttcctt ttcaaaatgt ccgctgtcga agcctatatg tgcataattg
     4321 tggataactc aagttctgtt ttctgttttt ctatatttaa ttcgatatat cattgaattt
     4381 aaagtatata aaaatgtata ataatgtgta taactatatc ttcttaatat ggaaaagtct
     4441 gttgtcggct ggatgtaggt ggcaaatcgg gtataatcgg cgcatctttt tccctttcag
     4501 acggcattga tgccgcaagg acatttttat gagcaaaaaa cgagttctga ccggcgtaac
     4561 caccaccggc atcccgcatc tgggcaacta cgtcggtgct atccgccccg ccgtccgcgc
     4621 ggcgcaaaac cctgataccg aatccttcct cttcctcgcc gattaccacg gtatcatcaa
     4681 atgccacgag ccggagatga ttcaccaatc cacccaagcc gttgccgcca cttggcttgc
     4741 ctgcggactc gaccccgagc gcaccacctt ctaccgccaa agcgacattc ccgaagtgat
     4801 ggaattgaac tggattctga cctgcatcac cgccaagggt ttgatgaacc gcgcccacgc
     4861 ctacaaagcc gccgtgcagg caaatgcgga aaacgggcag gaagaccccg atttcggcgt
     4921 ggaaatgggt ttgttcagtt atccgattct gatgactgcc gatattctga tgttcaacgc
     4981 caacgaagtc cccgtcgggc gcgaccaaat ccaacacgtc gaaatggcgc gcgacatcgc
     5041 cggccgcttc aatcaccgct tccgagaact cttcaccctg cccgaagtga aaatcgacga
     5101 aaacgtcgaa ctcttggtcg gtttggacgg acgcaaaatg tccaaatcct acggcaacac
     5161 cattccgctt tgggaaaacg acaaaaaaac ccaaaaatcg gtcaacaaaa tcatcaccaa
     5221 tatgaaagag ccgggcgagc cgaaacagcc cgacgaaagc ccattgtttg aaatctacaa
     5281 agccttctcc acgccgtctg aaacggtgga atttacgaaa atgcttgccg acggcctggc
     5341 ttggggcgag gcaaaaaaac ttttggcggc gaaaatcaac gccgaactcg ccgaaccgcg
     5401 cgaacgctac aacgagctga ccgccgaccc ttcgcaaatc gaagagattt tacaggcagg
     5461 tgcgcaaaaa gcgcgtaaag aagcacgcga attgttggac aaagtacgcg atgcggtcgg
     5521 catccgcccg ttgaaataaa cccgatgccg tccgaacctc ttaaaggctt gccggaacaa
     5581 aacgtcgtcc ggctgaccgg caagcatccc aacgacttgg aagccgtcgt cggcaaatgt
     5641 atggaaaccg acggaaaggg cgcgccttcg ggctgggcgg caaacggcgt gtgccatacc
     5701 ttgtttgcca aactggtggg caatatcgcc gaagacggcg gcaaactgac ggattacctg
     5761 atttcgcatt ccgccctgca accctatcag gcaggcaaaa gcggctatgc cgccgtgcag
     5821 aacggacgct atgtgctgga aatcgacagc gaaggggcgt tttatttccg ccgccgccat
     5881 tattgaggta ttcggacatc ccggaatata tttgggtttt tcaaaccctg cgggaaaaaa
     5941 gtccgcaccg tcgggaaact caaaaggagg gggatatgtg ttacaatttt ccgaactgtt
     6001 ttcataaaat agttttcgga cgtgtttcaa tatggcattg atgccgcctt attgttcgag
     6061 aaaaaaaacc tttatattta aatataatgg gttttaacta aacgggaaac cgttttctct
     6121 ccggccgatg ggcaaaatca gccgattgat ggaacacggt ccgattttta agcaaaacct
     6181 taatcaacaa ctcaagaagg ataagacatg aaaaaattat tgattgccgc aatgatggcg
     6241 gctgccttgg cagcttgttc gcaagaagcc aaacaggagg ttaaggaagc ggttcaagcc
     6301 gttgagtccg atgttaaaga cactgcggct tctgccgccg agtctgccgc ttctgccgtc
     6361 gaagaagcga aagaccaagt caaagatgct gcggctgatg caaaggcaag tgccgaggaa
     6421 gctgtaactg aagccaaaga agctgtaact gaagcagcta aagatacttt gaacaaagct
     6481 gccgacgcga ctcaggaagc ggcagacaaa atgaaagatg ccgccaaata atttgttgcc
     6541 ttggcaaaaa tgatgggatg ccgcctgccg gcaacccaaa cgaaaccgcc tgaagatttt
     6601 caggcggttt ttgggttatg tgccccttgt tttttacgcc tgcatgaccg ttccgagcag
     6661 atgtgccgcg catatcggga tgttgcggac aggcaaatgc cgtctggaca gggtttggac
     6721 ggcatttgtt ccgtaaggtt cagacggcct cgactttaaa cggcatattg attttttgcc
     6781 attggacgct ttcatattcc aatgcgtcgg caatcagggg gtggcgttcc agccattccc
     6841 tgtcaatacg caggatgaag ccgcagcttt ccgtatccgt gcgcaactgc atgtttttcg
     6901 ggaaagacag gtcttggcgc gaacggcaga acagtgcggc aaggcgcagc gacaaaacgg
     6961 cataccacaa catttcgttg gtgccgatga tgccgctcat ttttttcata tcgccgcgat
     7021 gaccgatgac cagttgggca agtatggtct gttctttgcg tgagaaaccc ggcatatcgg
     7081 cgttttcgag gatgtaggcg gaatgcttgt gatagccggt gtgggcgatg tccaaaccga
     7141 tttcgtgcag cgcggcggcg cgtccgagat actgttgcca caaggcaagc tcttgaaccg
     7201 taacattttt agcgtggcag aggctgtcca taaaggtttg cgcggtctcg gcggtgcgtt
     7261 tcgcctgatt gaggctgacg tggtagcggt gttggaactc ggcaaccgtt tgtccgcgca
     7321 tatcttcgtt taaaccgcgc ccgatcaaat cgtaaaacac gccgtcgcgc agggcggctt
     7381 cggttacggt catcctgtcg agtttcattt cttcaaacgc cgccatcatc acggcaagtc
     7441 cgccggcaaa aacttcgatg cgttccggtt tcaggttttc aaatttggcc tttttgaccg
     7501 aaccggcttc gatgatgcgt tcggcgaggg cgcgcatgcc tttgtaggta atgtccgcct
     7561 cttggggcat ttcggcggca agcacgtcgc ggatggattt tgccgaaccc gatgtgccga
     7621 cggcgaaatc ccaaccttcg cgcctcatat ttttgctgat gcgctggatt tcgttgcggg
     7681 cggcggaaat ggcagattgg aagtctttgg cggtgatttt gttttggaag aagcgcaggc
     7741 tgtaggttac gcagcccaag ggcaggcttt cagtaatgtc gggattcagc gtcgagccga
     7801 tgacaaattc tgtcgaaccg ccgccgatgt cgataaccag cattttgccg ccgcccgggg
     7861 ggagggtgtg gatcacgccg gtataaatca gccgcgcctc ttcgcgcccg gcgatgattt
     7921 cgatggggaa acccagtgcc gcttcggcct tgggaaggaa atctgcgatg tttttggcga
     7981 cgcggaatgt attggttgcc acggcgcgca cctgttcagg gcggaagccg cgcaggcgtt
     8041 cgccgaattt tgccaggcag tccaaagcct gttcttggga agcggcactc aggtttttct
     8101 gttcgtccag accggcggcg aagcgcacca tctgtttgaa cgaatcgata acttttaatt
     8161 gtccgttgtt gttttcgcaa atctggaggc ggaaactgtt ggaacccaaa tcgacggagg
     8221 cgaggacgtt tgcgggatgg gtggtcatgg tggataccgg tgggggaaaa acgcaatgtt
     8281 actctgacgg cgcaggcgtt gacaataaat gatgcggcgg tttttgattc tgcccacgga
     8341 tgttgccgac ggcatttttg cgcttatttg aaatcctttt ccacgctcat gaaaatctgc
     8401 gtgtttttgc gtgtgtaaaa acttttcata ttgctgtcga ttttcagata gcggaaattg
     8461 agttgcggcg taaagccctt ccaagagatt ttgtcgtgcc acaacgacag gtttgcctga
     8521 tattcgtggt ctttgcgcgg gaagcggtac acaatggtcc cgggtgcgtc aaacatcctg
     8581 cgggtatagc gcaggtttgc ccgcagaccc aagcccgccg tcgaacgttt tgactgcgcc
     8641 gacacgcaaa cccttgcgga cggaagcctg ttccgcctct ttcgtcatgt tgtgcgacca
     8701 gtccgcaccg ccgtaaagca gccagtcttt cggcgcggaa tacatcagcg tcgcgcccgc
     8761 cagcggcata tggctgtcgt atcgggcggc ggtgcggtct tcctgataac gcttccacat
     8821 attgcccacg ttcagcgtca gccgccagcg ttcgctcaag cgtcgggaga aatcggcatt
     8881 gaagccgccg acgaaattgt atcggctgcc gcctaagagg ttttgcccga taaacggcac
     8941 gatgccgaac gagcgcgtta ccgaacgatt tttatagccg agcgacaggc gcaggcactg
     9001 ttcgctgaaa tctttgttat cccaataatg cacgccgccg ccgctgatgc cgccgtagag
     9061 gaaatgatgc ccgcccgcat tgatttcgcg cgacacgcct aagccctagc gcaaaccgtg
     9121 tgccttttgc ggcaggctgt cggcggtttt cgtccagctt ctgcccgcaa attcaatcgt
     9181 ttttttggac gaagcgttgt ttatag
//
LOCUS       DAXHMX010000083         8929 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHMX010000083 DAXHMX010000000
VERSION     DAXHMX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.083"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            165..944
                     /gene="pgeF"
                     /locus_tag="ACFM4S_001383"
     CDS             165..944
                     /gene="pgeF"
                     /locus_tag="ACFM4S_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6051044.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAESLGGTPNADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            996..1475
                     /gene="lptE"
                     /locus_tag="ACFM4S_001384"
     CDS             996..1475
                     /gene="lptE"
                     /locus_tag="ACFM4S_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6051045.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1477..2475
                     /gene="holA"
                     /locus_tag="ACFM4S_001385"
     CDS             1477..2475
                     /gene="holA"
                     /locus_tag="ACFM4S_001385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6051046.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEINKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2596..3015
                     /locus_tag="ACFM4S_001386"
     CDS             2596..3015
                     /locus_tag="ACFM4S_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051047.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3090..3605
                     /locus_tag="ACFM4S_001387"
     CDS             3090..3605
                     /locus_tag="ACFM4S_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6051048.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3689..4573)
                     /locus_tag="ACFM4S_001388"
     CDS             complement(3689..4573)
                     /locus_tag="ACFM4S_001388"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6051049.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGNQSLELNRDLVGQLAEINKDLR
                     KHYDLAKLGVADILRFPGVLASQRENADALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5057..5920)
                     /gene="rpoH"
                     /locus_tag="ACFM4S_001389"
     CDS             complement(5057..5920)
                     /gene="rpoH"
                     /locus_tag="ACFM4S_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6051050.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYKPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6184..7722)
                     /gene="lnt"
                     /locus_tag="ACFM4S_001390"
     CDS             complement(6184..7722)
                     /gene="lnt"
                     /locus_tag="ACFM4S_001390"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6051051.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7791..8517)
                     /locus_tag="ACFM4S_001391"
                     /pseudo
     CDS             complement(7791..8517)
                     /locus_tag="ACFM4S_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8711..>8929
                     /locus_tag="ACFM4S_001392"
     CDS             8711..>8929
                     /locus_tag="ACFM4S_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC6051052.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSACLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYS"
BASE COUNT         1976 a         2229 c         2613 g         2111 t
ORIGIN      
        1 taagggcatc attcccgcac cggcaagaat ccgaacccga acgtttgaaa acaatcccga
       61 atctccgaat tcccgcctgt gcgggaatga cgaaaaaaca aacatccatt tgccccgaag
      121 gcagttaatc aaccctttcc gccacacacc tattccaata tccaatgaaa accatcacag
      181 aaaccctaaa tctcgccccg aaaggcaaaa acttcctgac cgccgattgg cccgcgcccg
      241 ccaatgtgaa aaccctgatt accacgcgca acggcggcgt gagccaaggt gcgtatcaga
      301 gtttgaacct cggtacgcac gtcggcgaca atcccgaagc cgtgcgccgc aaccgcgaaa
      361 tcgtgcaaca gcaggtcgga ttgcccgttg cctacctcaa tcaaatccac agcaccgtcg
      421 tcgtcaacgc tgcagaatcg ttaggcggta cacccaatgc ggacgcttcc gtggacgaca
      481 cgggtaaagc cgtctgtgct gtgatgaccg cagactgtct gcctgttttg ttttgcgaca
      541 gggcaggtac ggcggttgcc gccgcacacg cgggctggcg cggtttggcg ggcggcgtat
      601 tgcaaaacac cattgctgcg atgaaggttc cgcctgtcga aatgatggcg tatctcggcc
      661 ctgccatcag tgcggatgcg tttgaagtcg gacaggacgt gtttgatgcg ttctgcacgc
      721 ccatgcccga agccgccgcc gcatttgaag gcataggcgg cggcaaattc cttgccgacc
      781 tttacgcgct cgcccgcctg attctgaagc gtgaaggcgt gggcggcgta tatggcggca
      841 cgcattgtac ggttttggaa cgggacactt tcttttccta ccgccgcgac ggagcgacag
      901 ggcgtatggc gagcctgatt tggctggacg gcaatgccgt ctgaacacgc cgctgatata
      961 atctaccgac tttgtgcttt tgagaaaggc aagccatgaa caaactgttt cttactgccg
     1021 cagtgctgat gctgggcgcg tgcggtttcc acctgaaagg tgcagacggc atttctccgc
     1081 cgctgaccta ccggagctgg cacatcgaag gcggacaggc attgcagttt cctttggaaa
     1141 ccgcgctgta tcaggcttcg ggcagggtgg acgatgctgc cggcgcgcag atgaccctgc
     1201 gtatagacag cgtttcccaa aacaaggaaa cctacaccgt tacccgtgcg gcagtcatca
     1261 acgaatatct tttgatattg acggttgaag cgcaggtatt gaaacgcggc gagccggtcg
     1321 gcaaaccgat gaccgtgtcc gtccgccgcg tccttgctta tgccgacaac gagatcttgg
     1381 gcaaacagga agaggaagcg gcattgtggg cggaaatgcg gcaggatgcc gccgaacaga
     1441 ttgtccgccg cctgaccttt ctgaaggcgg aatgacgtgg cggcacatat cggacgcatt
     1501 gatacggacg cgcctttgaa acccctgtac gtcatccacg gcgaggaaga actgttgcgt
     1561 atcgaggcat tggacgcatt gagggcggcg gcgaagaaac aaggttacct taatcgggaa
     1621 gtttatacgg cagacaatgc tttcgattgg aacgagctgc tgcaaaccgc aggcagtgcg
     1681 ggtctgtttg ccgatttgaa gctgttggaa ctgcatatcc ctaacggcaa gcccggcaaa
     1741 accggcggcg aggcgttgca ggattttgcc gcccgcctgc cggaagatac ggtaacgctg
     1801 gttttactgc ccaaactgga gaaaacccag ctccagtcca aatggtttgc cgcattggcg
     1861 gcaaaggggg aagtgtggga agccaaaccg gtcggcgcgg cggctttgcc ccaatggata
     1921 cgcggacggc tggacaaaat cggtttgggt atcggggcag acgcattggc actgtttgct
     1981 gagcgcgtgg aaggcaatct gttggcggca cgtcaggaaa tcaacaagct cgggctgctg
     2041 tatccgaaag ggcataccgt caatatcgat gaggcgcaaa ccgccgttgc caacgtcgcc
     2101 cgcttcgacg cgttccaact ggcaggcgcg tggatgaagg gcgatgtccc gcgcgtatgc
     2161 aggctcttgg acggattgcg ggaagaaggc gaagaaccgg tactgcttct gtgggcggtt
     2221 gccgaagacg tgcggacgct gatccggctt gccgccgccc tgaagcaggg gcagagcatc
     2281 caatccgtcc gcaacagtct cagactttgg ggtgaaaagc agacgctcgc gccgcttgcg
     2341 gtcaagcgga tttccgtcgt ccgcctgctt gacgcgctca aaacctgcgc ccaaatcgac
     2401 cgaatcatca aaggtgcgga agacggcgac gcatggacgg tattcaaacg gcttgtcgtg
     2461 tcgctggcgg aataaagcgg taatccctaa aatccgaaaa tactgtaaaa taccgttaat
     2521 cctgaaaagt attcaccaat ccgtccgaaa acatttcaga cggcacgacc acctcaataa
     2581 aggaacatca accctatgga caataagacc aaactgcgct tgggcggcct gattttactg
     2641 accaccgccg ttttaagcct cattatcgta ttgattgtcg attcctggcc gcttgccatc
     2701 ctgcttgccg ccgtcatcgt cgccgccgct gcgggcggct ttgtttggac atcccgccga
     2761 cagcaacgcc agtttatcga acgcctgaaa aaattcgaca tcgatcccga aaaaggcaga
     2821 atcaacgagg caaacctgcg ccgtatgtac cacagcggcg gacaacacca gaaagatgcg
     2881 attaccctga tctgcctgtc gcaaaaatgt tcggtggacg aggcgcacgc tatgttcaaa
     2941 aaacgcccga cacgtcagga aatcaatcaa atggcggcaa aacagtcgcg cggtcagaaa
     3001 cgtccgcacc gttaaccaca tgagacatat ctgcatgaat gccgtctgaa gcctgttggc
     3061 gtttcagacg gcatattccg attgaaaaga tgatgatact gaaaaccgcc ccgctcaaac
     3121 gccgctttgc cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag
     3181 cattgtttgc cggtattgcc gccatttttc tgaatcccgt ttctattgcg gtttctgcat
     3241 tggtaacgag tatcctgata atgggatcat ggtggcttta tttccgtgcc aactggcacg
     3301 ggcagggaca gaccttggcg atgaggacgt ggaaaatcgg tttgtgcgac cttaacggca
     3361 tacagccgtc tctacactta ctccgcctgc gctttatttg ggcgtgcata ttcatcgtct
     3421 tcattcctat gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg
     3481 cggccggcgc ggcattgatt tggctgattt taccgtgggg gttcgccctg ctgaatcccg
     3541 atcggcagtt tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc
     3601 cttaagcctt tataccgcaa aggtttcaaa ctgaaaaaat gccgtctgaa agggctttca
     3661 gacggcattt gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct
     3721 gttcgatcag gactttcagc tcgaccgagg cttgggtgca ttcggtagcg atggatttgc
     3781 tgcccaaagt gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc
     3841 tgctgccttt gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc
     3901 tgaactcttc gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt
     3961 cgttgtcgat gctgccgacc gcttcggcaa ggcgggcgcg gattttttct ttatgtgttt
     4021 ccaacagggt aggaaagagt tcgcttaatg catctatgat ttcttccatg ctttcgaggc
     4081 gttgtaacag gtgctcgccc aattttttcc cttcgcgccg gcgtgcggcg gtaaagtcct
     4141 tcagtgccga atcggtcaaa tcggcaataa cctttgccaa tgcgtccgca ttttcccttt
     4201 gacttgccaa tacgcccgga aagcgcaaaa tatcggcaac gcccagtttt gccaaatcat
     4261 aatgcttgcg gaggtctttg ttgatttcgg caagctgtcc gaccagatcg cgattcagtt
     4321 ccaaagactg attgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc
     4381 gtgcgatatg ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg
     4441 gcatcctgat ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc
     4501 gtttgctgcc gcactctgcc gctgcgttgg caaatccggt catgctgtgg atgtggatat
     4561 ttccgctgct catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt
     4621 atggcggcaa gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc
     4681 acgcgctttt gttgctgcaa ggaagggaaa tgtatagtgg attaaccaag accagtacgg
     4741 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat
     4801 cggttccgta cgatttgtac tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc
     4861 cactatatca attccgccaa tctgtcggaa aagcagctga tgcggcagtg tctggtgcat
     4921 gtctgctttt tgatttcggc aattgcaacg gcgtggacgg ataaaatcgt gtacagcacg
     4981 acgcacaaac cgcattgatg tttaccaaat aaaatacccg acaaaacaat ttgtcgggta
     5041 ttttattgcg tatatttcaa accgcttcgg cttcttcggt caggaaacca cgcagtttct
     5101 gcatggcttt tgcttcaatc tggcggatgc gctcggcaga gacgccgtat tctgcagcaa
     5161 gctggtgcag cgtcagcccg ccgtcgtctt gaagccagcg gctttccaca atacggcggc
     5221 tcctgtcatc cagttgcgcc aaagcgtttt gtaaaccttc tgtttgcagg gcgtaatgcg
     5281 cctgtttcga tagttgccgg ctcggttcgg aatcgtggtc ggcaagccag tcgatggggg
     5341 cgaaactgtc ctcgtcgtcg ctgttgtctg ccatgatggc gatgtcgtgt cccgtcatgc
     5401 gctgttccat ttccagaact tcggaaagtt tgacacccaa atcgtcggcg atgtcttgtg
     5461 cctctttggg agacagggcg ttgaggtttt tacgcatact gcgtaaattg aaaaacagct
     5521 tgcgttgcgg tttggtggtg gcaacgcgaa ccaaacgcca gtttctcaaa ataaactcgt
     5581 ggatttcggc tttaatccag tgtacggcaa atgaaaacag acgcgcgcct ctaccgggct
     5641 tgtagcgttt gaccgccttc atcagtccga tattgccttc ctgaatcagg tctgcctgat
     5701 tcagcccgta gccgtcatag ccgcgcgcga tggaaacgac gacgcgcagg tgggacagga
     5761 tgagttgttt ggcggcgttg aggtcgccct ttatccggcg ttcggcgagg cgggtttctt
     5821 cttcctggga cagcatcggg atgctgttga cggtgtggat gtattgttcg aggctgccgt
     5881 tgccgctttg gatggcgggt aatgcgaaag cgttattcat ttggggcatt tcctttcggc
     5941 tgaaactgcg tatcggcggt ttgctgtgtt gggatgcagt atatcactgc ttggcttgta
     6001 ttttgtatgt ttggcaggag atatgcgcta aggtttgaaa gacaggaaaa ttttgtaagg
     6061 caggtttgat tgattttgta aacctgatga ctcaattcga tttcagaatt atattacata
     6121 cgtagttgta tgtaaacggc cggtttgaaa aaagacagcc cgtccggacg ggctgtgcag
     6181 gtatcagtgt tctttgtttc ggaagatgaa aagaatcagt gcggctaggg tcaatatgcc
     6241 catcaaccac catgaactgc cggttttcat atagggcgtt tcgccgacat agcctttgat
     6301 gtgtccttcc aatacggttt ccgtatcggg ttgggcttgg gcgatgatgt tgcctttggg
     6361 ggagatgatg gcggttgcgc cggtgttggt ggcacggacc atatagcgtc cgagttccat
     6421 agcccgtgcc tgcgattgtt ggaggtgctg gtacatggcg ttggattttc cgtaccacgc
     6481 catattgctg gcattggcaa gcagggtggc atctttggcg gcggcaatca gttcgtcgcc
     6541 gaatccgtct tcgtaacaga tgttgaaggc gattttttgg tttttcatca gcagggcgga
     6601 ttgcttaccg ccgcctttgc ggaagtcgga aaggggcata ttcatcattt tgtaaagcgg
     6661 cgtggtcagg aagggcagcg gtttgtattc gccgaagggg acgaggtggt ttttggcgta
     6721 gtaggggatg ccgtcctgat tgttttcctg ataaccggtc aggttgatga cggcgttttc
     6781 gtaaccgttg ccgtccgaag tgtattggct gatgccgacg gcgagcgcgc tgccgttgtt
     6841 ttgcgcctgt tcggcaaatt ttgccagtat gttttccggc aggttttggc gcataacggg
     6901 gatggcggtt tcgggcagga tgacgatgtc ggcagtggtt ttgccgacct gttcgtaata
     6961 tttctgtatg gtcgggatga cttggtcttc acgccatttg agggtttggt cgatgttgcc
     7021 ttgaagcagg gcgacggtgc tgcggctgcc gtcggggcgg gtgaagtcgg tttgtcgggc
     7081 ggtgtagcct gcggcaagca gggcggcaat caggataatc ggaagcaggc gtttgcccga
     7141 acgtgcggtg ttatcactcg ccaaaaccag ccagacaccg agaaaggcgg ttgccagtgt
     7201 aaccatgtgg atgccgccca atggggcaaa gccggcgagc gggctgtccg gggtgatttg
     7261 ggagtagccg attgcgcccc agccgaatcc ggtcaggaaa cgctcgcggg caaactcggt
     7321 cagcgtccac aggatgggca gtaccaaacc gatttttatg ccccgaggca gggtaaattt
     7381 tttccacagc cagaaacaca gtgccggata aagggcaagg taggcgggga gtaggaaggt
     7441 cagcggtacg gcatagaggt cgggcaggcc ggaaacgtcg tgcagggcgg tgtgtatcca
     7501 gtagaactgt gtcgtgtatg cggtcaggcc gaacaggtag gcggaagaga cagcaaaacg
     7561 cggacgcagt tcgatgaggc ggacgaaggc accgaaaatc aagggcatca gccaaaagtg
     7621 gtagtagggt gcgaaggtaa agggggtggc ggcggcaaaa aggatgagca aaggccagta
     7681 gagggcgggg tgctgccagt atttgtccag tttggaaacc atattcatct gtctgttcgg
     7741 aagataccgt ctgaacatct ttcaaacggc atcggtattt gaaaaagaaa tcaatgcctg
     7801 ccgaaacgat tcatcagcgg caaggcgggg gcgcaggcaa tcgaaccgga aagcagcccg
     7861 acaacgaggt tggcgaagta ctccgcccag ccagcgtccc agtgttgcgc gtgcaggaaa
     7921 tcgtgcagaa ggccgaggtt gtgggcgacc agtacgccgc cgataagaaa catggcaagc
     7981 gtgccgacca cgctcaaacc gcgcataaag caaggcataa aggcagtcag catttgcccc
     8041 aaactgcgcg aaaaggtttg tgggcggcgc atcagcagca tgcctaagtc gtcgagtttg
     8101 acgatgacgg caacgattcc gtacaccaaa acagtcatgc cgatgccgat tgccgccatt
     8161 acgagcatgc gcgtcatgtc ggtgcagaaa cttgtgcagc agcttttcta cgccttcaaa
     8221 gcacagataa atgccgcctg ccgtcaaaag cggcgtaatg agttgcggca ggaaggcgga
     8281 aagcagcagg gccgcaggca ccaaaaccgg cttgttggaa aaagaacctt tcgccatcga
     8341 ccaaacaatc ggcaactcgc gttctgccga tacgcccgta acccggttgg cattgggtgc
     8401 caaatcgtcg ccgaccatgc cggcggtttt ctttgcggcg gctttggtca tcagggcaac
     8461 atcgtccaaa acggcggtga tgtcgtccgg cagggtaaat agtgaggcaa atgccattaa
     8521 agaatcctga aatgcggcgc aaagtccgac attatatagg agaacgcgga tttgggcggt
     8581 ttcaggcggc atgaaacagg aaaatgccgt ctgaaggcgg tggcggacgt gaagtaaagt
     8641 ttcgtgaaaa aaatgccgtg ttacagtctt tcgattttaa tttaatgaat tttaagggag
     8701 aatcgttagc gtggattgga tgggcagtct gttcctgccg ggtggcgcac tgttgttcct
     8761 gagcgtggtt tcgaccactt tgtccgcatg tttgggaatg cctttgctgc tggtttctcc
     8821 cgccggcgtg ttggacgggg cggcggaagc cttggcgatt gcggcgttcc tgatgctggt
     8881 cgcgcgtccg ccggcagtgt tcggcggttt gtggaaattc aattacagc
//
LOCUS       DAXHMX010000084         8787 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHMX010000084 DAXHMX010000000
VERSION     DAXHMX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.084"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(175..867)
                     /locus_tag="ACFM4S_001393"
     CDS             complement(175..867)
                     /locus_tag="ACFM4S_001393"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6051053.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="ACFM4S_001394"
     CDS             complement(975..2168)
                     /locus_tag="ACFM4S_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6051054.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFM4S_001395"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFM4S_001395"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6051055.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="ACFM4S_001396"
     CDS             complement(3021..3950)
                     /locus_tag="ACFM4S_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6051056.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFM4S_001397"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFM4S_001397"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6051057.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFM4S_001398"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFM4S_001398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6051058.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNATLESGENQAGVGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7342..7881
                     /locus_tag="ACFM4S_001399"
     CDS             7342..7881
                     /locus_tag="ACFM4S_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6051059.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8071..8286)
                     /locus_tag="ACFM4S_001400"
     CDS             complement(8071..8286)
                     /locus_tag="ACFM4S_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051060.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8410..8769)
                     /locus_tag="ACFM4S_001401"
     CDS             complement(8410..8769)
                     /locus_tag="ACFM4S_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6051061.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2186 a         2433 c         2184 g         1984 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaaaccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatggg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt gatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tgcctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg cgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 ttagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg tccactgctt tgacctgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttggt
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagataatcg cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtacttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccatttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt gcccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa catgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggta aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggtgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catctctatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga atcccgaaca actttccgcc gtaacctggc cgccgcaatc
     4861 cgcccttgtg ctggcaggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgtatcgc
     4921 atggcttttg caaaccggtc aggcaagcgt gcacagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc agacccgttt gggcgcgatg attcccgtcg acgtccgcgc
     5041 tatgtggctc ggcacgttcc acggcctctg ccaccgcttt ctacggctgc accaccgcga
     5101 tgccggtttg ccgtcttcct ttcaaatcct cgacggcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtcttgagtg cgcccgatcc
     5281 gcacacaagc cgcatgattg aatgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat tttgccgaac tcatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccag cattaccaaa accgcttcaa ccacattctc gtcgacgagt tccaagacac
     5461 caacaaactg caatacgcct ggctcaaact catggcgggc ggcaacgcgg cagtgtttgc
     5521 cgtcggcgac gacgaccaaa gcatctaccg cttccgtggc gcaaacgtcg gcaatatgac
     5581 cgcgctgatg gaagagttcc atatcaacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 tgtcggcaac atccttgccg ccgccaatgc cgttatcgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatt cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctatcg cagcaacgcc caatcccgcg ttatcgaaca
     5881 aagcctgttc cgcagcggca tcccttacaa aatctacggc ggcctgcgct tttatgaacg
     5941 ccaagaaatc aaacacgcgc tcgcctacct tcgcctcgcc gttaatcccg acgacgacaa
     6001 cgccctcttg cgcgtcatca acttcccgcc gcgcggcatc ggcgcacgca ccatcgaaaa
     6061 ccttcagacg gcctcaaacg aacaaggcat caccctttgg caggcagcct gcaacgccgg
     6121 cgcaaaagcc accaaagtcg ccgccttcgt ccgcctcatc gaagccctgc gcaaccaagt
     6181 cggacaaatg cccctgcccg aaatcatcgt cggcatcctc aaagacagcg gcctgaccga
     6241 acactacaaa acccaaaaag gtgacaacca agaccgcctc gacaacctcg acgaactcgt
     6301 caacgccgcc atcgagttca aacccgaaga cagcaacttt gaaatcctgc ctgaaaacat
     6361 ttcagacgat cccgccttcc ccatcctcgc tttcttgagc aacgccaccc tcgaatccgg
     6421 cgaaaaccaa gcaggcgtgg gcgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggtatggaa gaaggccgtt tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcgcaa caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagagatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagaccg cctacgacag ctacggcagc ccgcgccaaa ccgccgcatc
     6781 caaagacaaa atcatcgacg actacaaaca gccccaaacc tacgcaggtt tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaacttcgg caaacagggc gtgaaagagt tggataccaa
     6961 gtttgcgaaa ttggaagcga tgtaaatttg aaatgcaggc tggatactcg tatccgacaa
     7021 aaatatttga cgcgtctatc gtttccgaaa aaccgctgtt ggaaaatgtc ggattcgaga
     7081 atccgaccta tggcaaaaaa ctgtagcaag gacaaggcaa aaggccgtct gaatccggta
     7141 acaacaaatt ttgccgaaac ctccgccgtc attcccgcgc aggcgggaat ccagaccttt
     7201 cggcacggga acttatcgga taaaaggttt ctttagattc cacgttctag attcccgcct
     7261 gcgcgggaat gacggacggt aaaagccggg tatagatacc taacccttgt ttatagctga
     7321 aaatcaataa ggaagaacat tatgtcccaa gtttttaaag attttgattt gtcctccgta
     7381 tgggaatccg acagttgggc agatgaaaac tacaaagaag ccccgtttac ccctgaaatt
     7441 ttggctgccg tagaaagtga actgggctat aaattgccgc aaagttttat tgaattgatg
     7501 gcggtacaaa acggcggaat atttgtcaaa aactgttttc cgaccacaca gagaaattcg
     7561 tgggcgaaag atcatgtgca aatttgcgag gtatcgggca tcggctttga aaaagaaggg
     7621 agtttgtgcg gcgaaacagg gcaaaaactt tggatggaag aatgggaata tccgcccatc
     7681 ggcgtgtatt ttgccaacga cccgtcaggc ggtcatgcca tgtttgcctt ggactatcgg
     7741 gagtgtggaa aagacggcga gccgaaagtg gtgtttgtcg agcaagaatc ggattttgaa
     7801 atcgtcgaac ttgcccccga ttttgaaacc tttatccgca gcttgcggca tgaagatgag
     7861 tttattgacg aggaaatata aaacggcggt tgaaaaaccg gaatcatcaa gggaaaacgg
     7921 gcgaaataac ggataatcgc ttgaatccgt aaggaaaacg gtttggtgga acgcgccatc
     7981 caagaccttt gcaaagaaaa actgtctccg acagcattga cattattaac agaacttatc
     8041 aagtttggag ctatctcaaa tataattcgg ttatcctttt gtatccatta aatcatatgc
     8101 ttcaattaat tgttgttcta gctcttgtac caattttgaa ttgcgaattc ctgacacaat
     8161 ctcaaattct tctgcatcta tgcaaacacc tgcataaatt tcaataacaa gggaacgcaa
     8221 taattgaagc tcttctcttg ttaaagaaat aataatgtca tcacctgtgt aattgattat
     8281 attcataata attttatttt tgtttgtcaa agtaagtttt gcctaaggtt ggtctaaatg
     8341 cagttccacc atcttttgaa tttgggtctc tgattacaat tgctccagac ttatcatccc
     8401 aaattgctct tatgtgtttg gattgtaatc ttcgaattcc caagaaaaaa atcgtaataa
     8461 gtttgaaagt gtcaaatccc aagtttcttt tgagcaatat tctaatattt tatcaatttc
     8521 acttttaata atcttatgat caaactgttc taatattaat gcattagacc aaaaaaaacc
     8581 ttctttatta caatgatggg aaatccattt aggagaacaa atgcaaagtg aaaaaataga
     8641 tgagccttgt tctccttcga ttccgatatc caaatctatc catctatgga aattatctgg
     8701 aatttcgggg gtaaattttt caaaatcaat atcatataaa tttatgcttt ttaaatccaa
     8761 tttaatcatt agggctgtcc tagataa
//
LOCUS       DAXHMX010000085         8692 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHMX010000085 DAXHMX010000000
VERSION     DAXHMX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.085"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            191..1726
                     /locus_tag="ACFM4S_001402"
     CDS             191..1726
                     /locus_tag="ACFM4S_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6051062.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="ACFM4S_001403"
     CDS             1723..1815
                     /locus_tag="ACFM4S_001403"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6051063.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="ACFM4S_001404"
     CDS             complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="ACFM4S_001404"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6051064.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDATKTIFSGVGKNEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3106..3276)
                     /locus_tag="ACFM4S_001405"
     CDS             complement(3106..3276)
                     /locus_tag="ACFM4S_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6051065.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDTARFGAIQTGLQL
                     QNKPQSAPQTRK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFM4S_001406"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFM4S_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6051066.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3697..4713
                     /locus_tag="ACFM4S_001407"
     CDS             3697..4713
                     /locus_tag="ACFM4S_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6051067.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKVSVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENINPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="ACFM4S_001408"
     CDS             4739..5158
                     /locus_tag="ACFM4S_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6051068.1"
                     /translation="MAQLPLYRTAEIENFTVGTPEVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAQGEWPKVTDRVLDDELQDKILSRKPT"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="ACFM4S_001409"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="ACFM4S_001409"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6051069.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVGNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLAHKVAVNKNEELT
                     LDEGLLNA"
     gene            5831..8620
                     /gene="polA"
                     /locus_tag="ACFM4S_001410"
     CDS             5831..8620
                     /gene="polA"
                     /locus_tag="ACFM4S_001410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6051070.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMAQLTAPDGAPTGAMYGVLNML
                     RRLRADYVHDYCAVVFDAKGKNFRHEMFPDYKATRPPMPDDLRPQAEALPDLVRLIGW
                     PVLVIPQVEADDVIGTLAAMAGEAGWNVVVSTGDKDMAQLVNERVTLVNTMSGETLDI
                     EGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAAE
                     IKGKVGENLRAALQRLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLVVDFKR
                     WGFRTWLKEAESRMHEAADGDLFGSDSIGEQAALNAEVPSEKQPELAPTPEKLDYQAV
                     TTEAQLAALLDKLSQADTIGIDTETTSLDAMNASLVGISIAFQAGEAIYIPVGHSLTA
                     APEQIDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYII
                     ESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALRL
                     EAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELSRQSAELGAELMKLEQ
                     EAYAVAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPLP
                     KIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPIR
                     TAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLITAFQNGEDVHRRTAAE
                     VFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKTFIDRYFARYPGV
                     AEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDLI
                     KRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDVP
                     LVAEVGVGENWEEAH"
BASE COUNT         2032 a         2277 c         2293 g         2090 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta ttcgttgccg tccaccaaca cctctgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggctccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     2101 ggaccgacga tgttggcggt cagaggctca atgtctttgg tttcgaccgc ctcgatgtga
     2161 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacgaat tcgacgcgtg tcagcagcga acctgcgttg
     2281 ccgaccaagc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaacggctt gggcatacgc gcccaaatca ggcacatttt cgtcttggta aacaatgccg
     2401 acgccgccgc ccaagtccaa gtgttccaac acaatgcctt cggcggcaag cgcgtcaacc
     2461 aaaatcaaaa tgcgctcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc aatgccgaat ttgttggctt tcagacctgt ggagatgtag
     2641 ggatgggttt tcgcatcgac atcgggattg acgcgtaggg agacggacgc ggttttgccc
     2701 aaacgcgcgg caaccttttg aatacggtcg atttcgggaa tgctttccat attgaagcat
     2761 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgt ttttgcccac gccggaaaaa
     2821 atcgttttcg tcgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctccccaa agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaacagg gtcatatctt cgttttcatt ttcgggtttg
     3121 tggggcggat tgcggtttgt tttgaagctg caaaccggtt tggattgcgc cgaaacgcgc
     3181 cgtatcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggcgg aaagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggtat cttaaacaaa aaccacacaa aaagccatga tgaccgaaag
     3361 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
     3421 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccgaaaacgg
     3481 cacgcaaatc atcgtcaacc gccacacgcc caaccaagaa ttatggattg ccgccaaaag
     3541 cggtggctac catttcgccg aacaaaacgg caaatggctg gcaacgcgcg acggacggga
     3601 tttttacgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg tagagattga
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggacttatat ttatcgcgat tattgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcaacacaa gtggaaaaag gtgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgttgtat ggttttcgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgcggt tgtcactgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagcg gttatggcgg cagagtctgt taccaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc atagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caagatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagtgt atgcgattgg ggaaaatatt
     4321 aatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagtg atggggtatc agaaaatacc
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
     4741 ggcacaatta cccctgtatc ggactgccga aatcgaaaat tttactgtcg gcacgcccga
     4801 agttttagaa tcattttcca aacatatccc ttatggtgtt gtctttgaag acgacggcga
     4861 cacaggctac ttctatgccg cttcgcaaga cgggatttta gatgccttgc acatttacaa
     4921 tgtcgaagat gtatccgata aacatatccc caatcatgtc ttgattttat gggatgatgc
     4981 ctgcaccata gccgcattgt gtatcaacga ctacattcat gccgtctatg attttgtcga
     5041 acaggcagga tattgccgca acggcttccc tgaagcacaa ggcgaatggc cgaaagtcac
     5101 agaccgcgta ttggacgatg aattgcagga caaaatccta tcccgaaaac ctacataacc
     5161 tactcaaaag gataacccaa tgcccctact agacagtttc aaagtcgatc acacccgtat
     5221 gcacgccccc gccgtacgcg tggcgaaaac catgaccacg cccaaaggcg acaccattac
     5281 cgtattcgac ctgcgtttct gcgtgcccaa caaagaaatc ctgcccgaaa aaggcatcca
     5341 cacgctggag catttgttcg ccggttttat gcgcgaccac ttgaacggca acggcgtgga
     5401 aattatcgac atttccccga tgggctgccg caccggtttc tacatgagcc ttatcggcac
     5461 gccttccgaa cagcaggtcg ccgatgcatg gctcgcctcg atgcaggatg tgggcaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgctcgcc gaagcgcagc aaatcgcgca aaacgtgttg gcgcacaaag tggcggtgaa
     5641 caaaaacgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatccgg ggttgtgcgg
     5761 ggtcgggttt taagccggca tcgtccttcc ctattttttc tgtcccttat cggttttaag
     5821 cgggtttttt atgtccaaca gacctacact cctcctcgtt gacggctcgt cctacctcta
     5881 ccgcgcgtat cacgcgatgg cgcagttgac cgcgcccgat ggtgcgccga cgggcgcgat
     5941 gtatggcgtg ttgaacatgc tgcgccggtt gcgggcggat tatgtgcatg attattgcgc
     6001 ggtggtgttt gatgcgaagg gcaagaattt ccgccacgag atgttccccg actacaaggc
     6061 gacgcgcccg ccaatgccgg acgatttgcg tcctcaagcg gaagccttgc cggatttggt
     6121 gcggttgata ggctggccgg tgctggtgat tccgcaagtc gaagcggatg acgtgattgg
     6181 cacgctggcg gcaatggcag gcgaagcagg ttggaatgtg gtggtgtcca ccggcgataa
     6241 ggacatggcg cagttggtga acgaacgcgt aacgctggtg aacacgatga gcggcgaaac
     6301 gctggacatt gaaggtgtga aggcgaaatt cggcgtgcgc cccgaccaaa tccgcgatta
     6361 tctcgcgctg atgggcgaca aggtggacaa cgtgccgggc gtggaaaagt gcggcccgaa
     6421 aacggcagtg aaatggctgg aagcctacgg ctcgctggcg ggcgtgatgg aacacgcggc
     6481 ggaaatcaag ggcaaagtgg gcgagaatct gcgtgccgcc ctgcagcgcc tgccgctgtc
     6541 gtatgatttg gtgacgatta aaaccgatgt ggacttgcac accgaacttt cagacggcat
     6601 cgaaagcctg cgccgtaccg cgccgaaatg ggcgcaactg gttgtcgatt tcaaacgctg
     6661 gggcttccgc acctggctga aagaagcgga aagtcgtatg cacgaagcgg cggacggtga
     6721 tttgttcggc agcgacagca tcggcgagca ggcggctttg aatgcggagg tgccgtctga
     6781 aaaacaaccc gaacttgccc cgacccccga aaaactggat tatcaagccg ttaccaccga
     6841 agcgcagctt gccgccttgt tggacaaact gtcgcaggcg gacacaatcg gcatcgatac
     6901 ggaaaccacg tcattagacg cgatgaacgc ctcgttagtc ggcatcagca tcgcgttcca
     6961 agcaggcgaa gcgatttaca tccccgtagg gcacagcctg accgctgcac ccgaacagat
     7021 tgatttgcaa gacgtattag gccgtctgaa accgcatttg gaaaaccccg ccctgaaaaa
     7081 aatcgggcaa aacctcaaat acgaccaaca cgttttcgcc aactacggca ttgccttaaa
     7141 cggcattgcc ggcgacgcga tgcttgcttc ctacatcatc gaaagccacc tcggacacgg
     7201 cttggacgaa ttgtccgaac gctggctggg cttggaaacc attacctacg aatcgctgtg
     7261 cggcaaaggc gcgaagcaaa tcagttttgc cgatgtcgcc atcggacagg cgaccgaata
     7321 cgccgcccaa gacgccgatt tcgccctgcg cctcgaagcg catctgcgtg cgcaaatgga
     7381 cgccaaacag cttgaaatgt atgaaaaaat ggagctgccc gtcgcgcagg tattgtttga
     7441 aatggaacgc aacggcgtgc aaattgatcg cgccgaactc tcccgccaaa gcgcggaact
     7501 cggcgcagag ctgatgaagc tcgaacaaga agcctatgcc gtcgcaggcc agccgtttaa
     7561 cctcaactcg cccaaacagc tccaagaaat cctgttcgac aaaatgggca ttcccaccaa
     7621 aggcctgaaa aaaaccgcca aaggcggcat ttccaccaac gaagccgtgc tcgaacagct
     7681 cgcacccgac taccccctgc ccaaaatcat cctgcaaaac cgcagcctgg cgaagctcaa
     7741 atccacctac accgacaaac tgcccgaaat gatttccccc aaagacggcc gcgtgcatac
     7801 cacctacgcc caagccgtcg ccattaccgg ccgtctcgcc agcaacaacc ccaacctgca
     7861 aaacatcccc atccgcaccg ccgaaggccg ccgcgtgcgc cgcgccttta ccgcaccgca
     7921 aggcagcgtc atcgtttccg ccgactattc ccaaatcgag ctgcgcatca tggcgcacct
     7981 ctccggcgac aaaaccctga ttaccgcgtt ccaaaacggc gaagacgtac accgccgcac
     8041 cgccgccgaa gtgttcggca ttgcccccga aaacgtctcg tccgaacaac gccgctacgc
     8101 caaaaccatc aacttcggtc tcatttacgg catgggccaa tacggcctcg ccaaatcatt
     8161 gggtatcgac aacatctccg ccaaaacctt catcgaccgc tacttcgccc gctatcccgg
     8221 cgtcgccgaa tacatgcagc gcaccaaaga acaagcagca gcgcaaggct tcgtcgaaac
     8281 tctgttcggc cgccgcctct acctgcccga catccgcaac aaaaacgcca acgcccgcgc
     8341 cggagccgaa cgcgccgcca tcaacgcccc catgcaaggc accgcctccg acctcatcaa
     8401 acgcgccatg atagacgtgc gaaactgtct ttcagacggc atcggcagca aactggtcat
     8461 gcaggtgcat gacgaactgg tgctggaagt cgttgaaacc gaactggatt ttgtcaaaga
     8521 aaaactgccg cagattatgg cgaaagtgga cggcggatta ttggatgtac cgctggtggc
     8581 tgaggttggc gtaggggaga attgggaaga ggcacattga ttgaaaggtg ttatatgcta
     8641 tctttattta aataaaattt aatttttggt atattttttc taaatgttcc ta
//
LOCUS       DAXHMX010000086         8618 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHMX010000086 DAXHMX010000000
VERSION     DAXHMX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.086"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..126
                     /locus_tag="ACFM4S_001411"
     CDS             <1..126
                     /locus_tag="ACFM4S_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6051071.1"
                     /translation="KFRYARAAYFGLLKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            complement(465..1025)
                     /gene="sodC"
                     /locus_tag="ACFM4S_001412"
     CDS             complement(465..1025)
                     /gene="sodC"
                     /locus_tag="ACFM4S_001412"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6051072.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1442..2482)
                     /gene="mutY"
                     /locus_tag="ACFM4S_001413"
     CDS             complement(1442..2482)
                     /gene="mutY"
                     /locus_tag="ACFM4S_001413"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6051073.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2645..3686
                     /locus_tag="ACFM4S_001414"
                     /pseudo
     CDS             2645..3686
                     /locus_tag="ACFM4S_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3709..3927)
                     /locus_tag="ACFM4S_001415"
     CDS             complement(3709..3927)
                     /locus_tag="ACFM4S_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051074.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3942..4580)
                     /locus_tag="ACFM4S_001416"
     CDS             complement(3942..4580)
                     /locus_tag="ACFM4S_001416"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6051075.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4761..6596)
                     /gene="edd"
                     /locus_tag="ACFM4S_001417"
     CDS             complement(4761..6596)
                     /gene="edd"
                     /locus_tag="ACFM4S_001417"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6051076.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            7112..8557
                     /gene="zwf"
                     /locus_tag="ACFM4S_001418"
     CDS             7112..8557
                     /gene="zwf"
                     /locus_tag="ACFM4S_001418"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6051077.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT         2023 a         2046 c         2382 g         2167 t
ORIGIN      
        1 aaattccgct atgcgcgggc agcctatttc gggctgctca aagtgagtgc gcaaagccac
       61 ctgaaagcga tgtgtttgaa cctgttgaaa gcggccaaca ggctaagtgc gcccgctgcc
      121 gcctaaaagg cagcccggat gcctgattat cgggtatccg gggaggatta agggggtatt
      181 tgggtagaat taggagtaat taggagcgaa aatagacgaa aacctgtgtt tgggtttcgg
      241 ctgtcgggag ggaagggaat tttgcaaagg tctcaaatag tacggaatcg attcacttgg
      301 tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ctgtactggt
      361 ttaaatttaa ttcactatac tgctgaagtc gtgaattaaa tgctaaatat ctagcaaaaa
      421 agtgcggtca aaatttaccg cacttttcgt ttcgaacaat cgaattattt aatcacgcca
      481 catgccatac gtgggccgcc accgccaagt ggagctggat gatcggagtg attatcacca
      541 cccgtgtgga tcataataga gtgaccgcga acatcatcta aatgtttaag acgtggtgct
      601 aaaacaggat ttgttgctgt gccatcatgc aatacagtta atgcaggtaa atcacctaag
      661 tgtgcatcat cttgccatgg gtaaccatgt tgttttgcac ctttaggatc ccagtgaccg
      721 cctgcgccta aaccagctgt caatttacct tctttttctt ttggctcaca gcttgggttt
      781 tcatggatgt ggaaaccatg taagccttcg cttaatcctt gtaaatcagg ggtaaacaca
      841 agaccatagt tagattcagt aatagtcact gtacccacat ctttgttacc gtttactgga
      901 tcaagttgtt gcactttcac ttcaatagaa gcacctttag gtatcgtatt atgctcgtgt
      961 gcttgcgcaa caccaactga acatactgca ctaaccgcta gtgctaataa ggttttcata
     1021 ttcattgcaa tctcctttat tgcagtaaaa actcgcgact attttagtca attttgtttt
     1081 aaaagccgat aaatttttac gaaaatttta cattaattgt tatttgcaga aaattagaac
     1141 ccgttatcac gtcttggata aatgccaaaa ccattgtgat tcagagaatt aaaaagaaaa
     1201 atttcggaat cctcatcctt ttctgaaacc tgattgcggt atccatggct ttgtctttgt
     1261 catttaagtt tgtgttcatg tttttgacct tctttgcttt ttaaatcctt tttaaattgc
     1321 cgacttctac cgttccaaat gtcccatttg aacattatcc cgccgcctaa aatacgaccc
     1381 aaattcatcg gttgttcttg ctgcgatacc tgcaaacaac agttcaatgg gtttattttg
     1441 tttattctaa cctattaccg tttaaataaa tttccaaagg cttgggcaaa ccgtaatctt
     1501 tcaaatgcgc cggctttatc caaatgccgt ctgaagggct ttcagacggc atttgtcctt
     1561 caaacggcgt aatcatcagc aaccggtgcg tcagccggtg ggtcagggcg gtttgttcgt
     1621 ccatatctgc catggtcagg gagaatttgg cggcaaagtc ggaaagcccg ttcaaacttt
     1681 caaaacacgg cacgcaatac agcccgcccc aaatgccttt ggcggggcgt ttttccagca
     1741 aaatcgcgcc gttccggttg cggataatca gccagtaaag cggcagggtc ggtacttcgg
     1801 cggcggtttt tttgcgcggc agctcggcgg tgcggttttg ctttttcgct tcgcagatgt
     1861 ccgccatcgg gcattggtgg cacaagggtt tcgtccgttt gcacacggtc gcgcccaaat
     1921 ccatcaaacc ttgtgtatag gcaggcatat cggcgttttc agacggcagc aggctttcgg
     1981 caagtgtcca gagcgagttt tcaaattttt tgtcctgcgg attgccgtcg cgggcgaaca
     2041 cgcggcagag tacgcgtttg acgttgccgt ccaaaatggt ttcgcggcgg ttgaaggaga
     2101 aggcgcaaat ggcggcggcg gtgcttctgc ctacgccgca gagggtttcc aagtctttgc
     2161 gctccgacgg aaacgtgccg ccgaattgcc tgacgacttg ttgcgcggct ttgtgcaggt
     2221 tgcgcgcgcg gctgtaatag cccaagcccg cccacaacga caacacttcg tcttgcggcg
     2281 cggcggcaag cgtctgaacg gtcgggaatt tttctaagaa gcgcggatag tagtccaaca
     2341 cggtggcgac ttgcgtttgc tggagcatga tttcggaaag ccagacgcaa taagggtttt
     2401 tgacctgcca agggaggtgg tggcgaccgt gttgtttttg ccagcggatg agccgttcgg
     2461 agaaggggat gggtgtgttc attaatatca atcggtggtt ttatttatat ttaaaacggt
     2521 atgttattgt ataacattgt gaaaataatt cttattgact tattttttgt aggggcatat
     2581 aactcatata aagaaacttt attgcggtat tgaaattatt tatcaacaag caaggagtat
     2641 cagcatgaaa gcaatggttt atcacggcgc aaacgacatc cgttttgaag aaaaaccccg
     2701 tccgcagatt atcgatccga ccgatgcggt ggtgaaaatc gtcaaaacca cgatttgcgg
     2761 taccgatttg ggtatttgga aaggcaaaaa aacccgaagt cgccgacggt cgtattctcg
     2821 gtcatgaagg catcggtatt gtagaagaag tcggcgatgc tgtaaaaaac atcaaagtcg
     2881 gcgataaagt cattatttca tgcgtcagca aatgctgtac ttgcgacaac tgcaaaatcc
     2941 aactttattc gcattgccgc aacggcggct ggattttggg ctacatgatt gacggcacgc
     3001 aggcagaata cgtccgcacg ccttatgccg acaacagcct cgtgccgctg cccgacaacg
     3061 tcaacgaaga agtcgccctg ttgttgagcg acgctttgcc gaccgcacac gaaatcggcg
     3121 tgcaatacgg cgatgtcaaa cccggcgaca ccgtattcat cgcaggtgcc gctcctgtcg
     3181 gtatgtccgc cttgttgacc gcccaactgt atagccccgc cgccattatc gtgtgcgata
     3241 tggacgaaaa ccgtttgaaa ctggcgaaag agttgggcgc gacccatacc atcagccccg
     3301 cttccggcga cgtctccaaa caagttttcg ccatcgtcgg cgaagacggc gtagattgtg
     3361 ccatcgaagc ggtcggtatc cctgcaactt ggaatatgtg ccaagacatc gtgaaacccg
     3421 gcggccatat cgccgttgtc ggcgtacacg gtcaatccgt tgatttcaaa ctggaaaaac
     3481 tgtggattaa aaagcttgcc atcactaccg gcctggtaaa cgccaatacc accgaaatgc
     3541 tgatgaaggc aatttccagc agctccgtcg attacaccaa aatgttgacc caccgtttca
     3601 aattcagcga attggaaaaa gcctacgacg tgttcaaaca cgccgccgaa aatcaagcga
     3661 tgaaagtggt tttggaagcg gattaatcgg cgttgaatca gttgtttttt aagcggatgc
     3721 cgtctgaatg cgtttcagac ggcatttttt cagccgcgta gtatgggctg tccggctgct
     3781 gtcgaatgaa ctgaacgtta tttttattat gcggaaggcg gatgggcatt ttaggggcag
     3841 tatgtcggag tggtgctgcc gtgtatgccg actgcgccgc caagcggcaa aatgccgtct
     3901 gagactggaa agggcttcag acggcatttt tacgatgcgg ttcaggcttt gggtttcaat
     3961 gccgccgctt ctttggcgag acgggtgatg gtgtcccagt ctttgttttt cacggcttct
     4021 ttcggcgtca gccatgagcc gccgacgcac aggacgttgg gcagtgccaa gtaatcgggc
     4081 gcggtggcga ggctgatgcc gcctgtcggg cagaagcgaa catcggcgta agggccgtac
     4141 agggctttga gcatggcttt gccgccaacg acttcggcag ggaagagttt gagggtgtcg
     4201 atgccgtgtt ccaaagccag ttggatttcg cccggggtgg caacaccggg aatcaggggg
     4261 atgccgctgt tgcggccggc tttggcgagg gattcatgca gccccgggct gatggcgaaa
     4321 accgcgcctg cgtcttcgac ggctttaagc tgttcgggat tggttaccgt acctgcgccg
     4381 acgatggcgt tgggcacttc tttggcaatc aggcggatgg catcgaggcc gacaggggtg
     4441 cgcagggtga tttcgagggt agggatgccg ccttcgacaa gggcgtggga caaatcgatg
     4501 gcggtgctta agtcgtcaat cgccattacc ggcacaactg cgccggcggt caaaatttcg
     4561 cggggggtca gtttggacat ttcggttctc cgggtggaat ggggtatttt attaagatgg
     4621 gacaggtcgt aggtcggatt caagaaaccg acatcttgga cggctgtgtc ggattcgaga
     4681 atccaaccta cgtctatctt aaaacacttg aataattttt cagacggcct tcggtctgaa
     4741 aaggccgtct gaaacgcgca tcaggcaaat tcgccgccga agctcatcgc gccggtttcg
     4801 gcgctgctgg tcatgctgcg gaagttggcg aaaagttcgc ggccgcagcc ttgttggttc
     4861 gcgcccaagt cgatgctttc gacttcgcgg gcattccatt cggtttcgtt aatcaggacg
     4921 ttgagttcgc cgctaacgga gtcgaagcgg atcaggtcgc cggtacggat tttggcgatg
     4981 ttgccgccca tcagggcttc gggcgtcatg tggatggagg ccggaacttt gccggatgcg
     5041 ccggacatac gaccgtcggt cagcagtgcc actttgaagc cgcggtcttg caggatgccc
     5101 aaaggcgggg tcagtttgtg caattcgggc ataccgttgg cacgcgggcc ttggtagcgg
     5161 acgacgcaca caaaatcgcg ttccaactcg ccgcgttcaa acgcagccaa cacttcgcgt
     5221 tggtcgttga acacgatggc aggcgcttca ataatgcggc agccttcgcg cacggcggac
     5281 actttaatca cgccacgtcc gatgttgcct ttcatcaggc gcagaccgcc gtcgggggag
     5341 aacgggttgt cagctttacg caggatgtcg tcgttgccgc tggtttcggg ggcttcgcgc
     5401 cattcgagtt tgccgtcgat gaggaaaggc tctttggtgt agtggcgcat accgtgtccg
     5461 acgacggtat cgacatcgtc gtgcaacagg cctgcattca gcaattcgcg gataacgaaa
     5521 ggcagtccgc ccgctgcggt aaagtgattc acgtcggcct tgccgttcgg ataaacgcgg
     5581 atgagtagtg ggatgatgga ggaaatttcg tcgaagtcgt cccagttcaa aatcacgccg
     5641 gccgcacgcg ccatagcgac gaggtgcatg gtgtggttgg tcgaaccgcc ggttgccatc
     5701 aggccaatca aggcgttgat aaaggatttt tcggtcaaca tttcgcccaa aggtttaatc
     5761 gtgccgtttt tgatgccgcg cgcgaggtgt ccggcggcgt agcgagtcag cgcttcgcgc
     5821 aggtcggtgt aagggtggac gaaggcggcg gcaggcaggt gcacgcccat catttccatc
     5881 atcatttggt tggagtttgc cgtgccgtag aaagtgcagg tgcccgggct gtggtaagaa
     5941 cccatttcgc ttttcaaaag ttcgttgcgt ccgaccttgc cttcggcgaa aagctggcgg
     6001 gtgcgggctt tttctttgtt gccgataccg ctggacatcg ggcctgcggg gacgaagata
     6061 cccggaatat gaccgaacga aagcgcgcct atcatcaaac ccggaacgat tttgtcgcat
     6121 acgcccataa acaggctgcc gtcaaacatt tgatgcgaca gcccgacggc ggtactcatg
     6181 gcaatcacgt cgcgggagaa cagcgacagt tccatgccgg cgtagccttg cgtgatgccg
     6241 tcgcacatgg cgggcgtgcc gccggcgact tgggcggtcg cgccgttttt ctgcgcttcg
     6301 tctttgattt ggtcagggaa gtctttaaac ggctggtgtg cggaaaccat gtcgttgtag
     6361 gcggtgatga tgcctaagtt ggggacggtt tcctgaagca tttcgatttt gatactttta
     6421 ggcatggcag catagccgtg tgccaagttg ctgcagccga gctggttgcg ctctaagcgt
     6481 cccatttgtt tggcactgcg gatcttcgcc agatattttt cacgcgtcgg acggctgcgt
     6541 tcaataatgc gcccggtgat ttcggcgagt ttggagtgga taggagtagt gttcacggtc
     6601 ggtctcctgt cggaacgggg ggtattgggg atttgtttgt gcgtttctga tttttgtatc
     6661 gggtattcag acggtaagta tagtggatta acaaaaacca gtacggcgtt gcctcgcctt
     6721 gccgtattat ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
     6781 atattttaaa aggtatgccg tctgaaagtg ggaagcgggt gttattatag tacaaaattt
     6841 gtaattttgt tacaaaaaaa tcagacatcg aaaagaaatt tgatttaaat cgcagaatct
     6901 ttcgattgtt gtaacggaat aggggaaacc tgcccgattt ttggactagg tcaaataaat
     6961 tgtaaaaatg ggaatagaca aaaagtgtag cggtgtgtag tattactact cattatgccg
     7021 cctgcctttg tttccgatat tgcgatgcag cgtgatgggg aaaacaggtt tcaaacggca
     7081 tcgaaaaatt ctttcctgac gagataatac gatgagtaca caaacaaatt ttgatttggt
     7141 gttgttcggt gcgaccggcg atttggcgat gcgcaagctg ttgccctgtc tgtaccaggc
     7201 gcatgttgcc ggtctgcttc atcccgaagg gcgtattttg ggggtcagcc gcagcgagtt
     7261 ggataccgaa ggttttctgg cgaaggtgga aaccagttcc aaaatccatg tgaaagaaaa
     7321 tttttccgat gaggcttggg catcgtttgt cgaacgtttc gcatatctca aagtcgatgt
     7381 tacgcagccg gacgattttg ccgctttggg cgatttggtc aaagcgcgca aagaaaccga
     7441 caatgtcgtc atctatcttt ccaccgcgcc gaaattcttc gcgcaaacat gtgaaaacct
     7501 tgccaaaatc ggtttgaacg ccgacaacgt gcgtgtggtt ttggaaaaac cgttgggtac
     7561 ggatttggct tcttcccagc aaatcaatac cgatgtcgcg cgttatttca aagaagggca
     7621 aatttaccgc atcgaccact accttggcaa agaaagcctg caaaacctgc ttgcgctgcg
     7681 ttttgccaat gtaatgttcg agccgctgtg gaacaataaa tacatcgaaa gcgtgcagtt
     7741 gaccatcgcc gagcagttgg gcgtggaaga acgcggcgag ttttacgaca ttaccggcgc
     7801 gttgcgcgat atggtgcaaa atcacttgat gcaaatgctg tgcatgactg cgatggaagc
     7861 ccccgccagc ttggatgccg acgcggtgcg cgatgaaaaa gtcaaagtca tcaagtcatt
     7921 gaagccgctg accgtcgaat ctgtcaatga aaatgtcgtg cgcggacaat ataccgccgc
     7981 caaaggcatg aacggctatc ttgaagaaat caacgttccg caagacagct ttaccgaaac
     8041 ctacgtcgcc attaaagccg aaatcgaaaa cgaacgctgg aagggcgttc ccttctacct
     8101 gcgtaccggc aagcgcatgg cgggcaaagt ggcggaaatc gttttgaact tcaaagattt
     8161 gaacagccat atttttgaag gcagccgcac cgcgcccaac cggctcgtta tcgagttgca
     8221 accatatgaa tccgtgcgcc tctatacgca gatgaaaacc ccgggggcag gaaataaggt
     8281 cgaaaccgtg ccgctggcaa ccgatttggg caaagcattg gaaagccgcc gcgcggaagc
     8341 ttacgagcgc ctgctgctgg atgtgattaa cggcaaactc gctttattca accgccgcga
     8401 cgaactcgaa gccgcgtggg aatatgtgat gccgattttg gaaaactgga caaataacac
     8461 cacgccgccg cacggctacg gcgcacactc gtgggggcct gaagccgcgc gcgaactatt
     8521 ggcgcgcgac ggacacaagt ggcacgaaga gcagtaatac aataatgcgt tcagacggca
     8581 tggggtttga aatgccgtct gaacgtaagt aaagcagt
//
LOCUS       DAXHMX010000087         8582 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHMX010000087 DAXHMX010000000
VERSION     DAXHMX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.087"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(105..479)
                     /locus_tag="ACFM4S_001419"
     CDS             complement(105..479)
                     /locus_tag="ACFM4S_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6051078.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNSRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(476..604)
                     /locus_tag="ACFM4S_001420"
     CDS             complement(476..604)
                     /locus_tag="ACFM4S_001420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051079.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            complement(652..1008)
                     /locus_tag="ACFM4S_001421"
     CDS             complement(652..1008)
                     /locus_tag="ACFM4S_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6051080.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(1013..1117)
                     /locus_tag="ACFM4S_001422"
     CDS             complement(1013..1117)
                     /locus_tag="ACFM4S_001422"
                     /inference="COORDINATES: protein motif:HMM:NF018014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051081.1"
                     /translation="MDDIRGIIQGAVNPLIYKVSKQKIIYNNKLISGT"
     gene            complement(1151..1495)
                     /locus_tag="ACFM4S_001423"
     CDS             complement(1151..1495)
                     /locus_tag="ACFM4S_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051082.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1654..2055)
                     /locus_tag="ACFM4S_001424"
     CDS             complement(1654..2055)
                     /locus_tag="ACFM4S_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051083.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2059..2511)
                     /locus_tag="ACFM4S_001425"
     CDS             complement(2059..2511)
                     /locus_tag="ACFM4S_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051084.1"
                     /translation="MQTKALGTVDEIGDTVQQVGKQASGQKTSGGNPAIDSDPYSPSS
                     VAARIEAGKARSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDGRAVNARNDSSGGEPTLEITISNNRKIKIRYGNTR"
     gene            complement(2506..2799)
                     /locus_tag="ACFM4S_001426"
                     /pseudo
     CDS             complement(2506..2799)
                     /locus_tag="ACFM4S_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2869..3186)
                     /locus_tag="ACFM4S_001427"
     CDS             complement(2869..3186)
                     /locus_tag="ACFM4S_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051085.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3183..3443)
                     /locus_tag="ACFM4S_001428"
     CDS             complement(3183..3443)
                     /locus_tag="ACFM4S_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6051086.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
     gene            complement(3441..3785)
                     /locus_tag="ACFM4S_001429"
                     /pseudo
     CDS             complement(3441..3785)
                     /locus_tag="ACFM4S_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(4211..4555)
                     /locus_tag="ACFM4S_001430"
     CDS             complement(4211..4555)
                     /locus_tag="ACFM4S_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051087.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(4593..4937)
                     /locus_tag="ACFM4S_001431"
     CDS             complement(4593..4937)
                     /locus_tag="ACFM4S_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051088.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(4940..5224)
                     /locus_tag="ACFM4S_001432"
     CDS             complement(4940..5224)
                     /locus_tag="ACFM4S_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051089.1"
                     /translation="MAGGNKSLKIGTQSVEKSTGRTIPNNLKEQLAMEEVKANPQGKT
                     PARIPPMSDTKNGWLAKDGWVKRVQNVNKIEIHYIENSRTGEKTDFKFKD"
     gene            complement(5198..5542)
                     /locus_tag="ACFM4S_001433"
                     /pseudo
     CDS             complement(5198..5542)
                     /locus_tag="ACFM4S_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(5626..5928)
                     /gene="imm40"
                     /locus_tag="ACFM4S_001434"
     CDS             complement(5626..5928)
                     /gene="imm40"
                     /locus_tag="ACFM4S_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC6051090.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(5954..6220)
                     /locus_tag="ACFM4S_001435"
                     /pseudo
     CDS             complement(5954..6220)
                     /locus_tag="ACFM4S_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6241..6447)
                     /locus_tag="ACFM4S_001436"
     CDS             complement(6241..6447)
                     /locus_tag="ACFM4S_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC6051091.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(6413..6853)
                     /locus_tag="ACFM4S_001437"
     CDS             complement(6413..6853)
                     /locus_tag="ACFM4S_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB"
                     /protein_id="HFC6051092.1"
                     /translation="MAGGNKPPKPSTQQHPTHSDNNIGLPASYVKPDTSISPTGTIQD
                     RIRWTKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHR
                     RVRYDINNNIYVLANPKTFKIKTMFKPNLGKKYYDGEFKKDMGN"
     gene            complement(6833..7162)
                     /locus_tag="ACFM4S_001438"
                     /pseudo
     CDS             complement(6833..7162)
                     /locus_tag="ACFM4S_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(7268..7639)
                     /locus_tag="ACFM4S_001439"
     CDS             complement(7268..7639)
                     /locus_tag="ACFM4S_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6051093.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(7636..7991)
                     /locus_tag="ACFM4S_001440"
                     /pseudo
     CDS             complement(7636..7991)
                     /locus_tag="ACFM4S_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8019..8225)
                     /locus_tag="ACFM4S_001441"
     CDS             complement(8019..8225)
                     /locus_tag="ACFM4S_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051094.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(8235..>8582)
                     /locus_tag="ACFM4S_001442"
     CDS             complement(8235..>8582)
                     /locus_tag="ACFM4S_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051095.1"
                     /translation="NRPPKSITSNSKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQ
                     EFTDLNINSPADFARHIENIVSHPTNMKELPRGRTAYWDDKTGTIVIRDKNSDDGGTA
                     FRPTSGKKYYDDL"
BASE COUNT         2458 a         1644 c         1351 g         3129 t
ORIGIN      
        1 ttaacaacag tgaaataaaa gttcttgttc cattttaaaa caattagtaa aatcgagttt
       61 atcctagttg tccagggcag cctcatctct ttatagatag ctgcttacca agtatcctta
      121 attgctaatt caagccaatc ttcaaaacta ttaaaaaatt catggttttc tatattatct
      181 aaattaatga caataaccct agaatttcct gttacatcat taccatcaaa ataagcttcc
      241 cattctccat tactagcgaa tgggataaga tttaaatcag gcaggataga atttctaaat
      301 atataagcta cttctgcccc ttctgttcca taattctcaa accaccagtg aaaattatgt
      361 ggctgaatat aaggtattaa agatgtatcg ttagagatac gaacatactt ttcaggataa
      421 caaaaccttt caggaagcat attactagga tataaattaa attcattttg aataatcatt
      481 tcgttacctt aggatcataa atactaccat taggaaactg ggtagaccaa tttggtggtg
      541 ctagttcatt ttcaatttgt tttttaattc taaattgaat agcttctccc catgtttgcg
      601 gcatctaatg ctgcttgctc tgcatcaata ttactaaaaa ttggattagt ctcaaaaatg
      661 aaaaattact gttttttcat tttttatagc atattcaagt acatgaataa tatttttcac
      721 taagggctct gtagaaaaat ctgaattttg taatttttct tttacagcca aaatactttc
      781 cttatctatt agccaatccc cctcacttgg tccaaataac caattatcgg taatttcaga
      841 aacaagtctc cataatccac gattttgata ttcaataatt tgattttccg taatttctac
      901 aactgtttca gattgaatgt ttttataagc ccaatcaatc atttcagtat attcactaat
      961 tgaaacattg tcttttaaac agaaaattaa caggttgtgt tcattcattt gtctaagttc
     1021 ctgatatgag tttgttgttg taaataatct tttgcttgct taccttgtaa attaaaggat
     1081 taaccgcacc ttggatgatg ccgcgaatat catccatacg gttaccccac caatttttat
     1141 tggtgtatcg ttaatcattt atttcaaaaa agttatttat tatcatatta tcagggagca
     1201 ggtcgttaaa aaaaaccccc tcaaattcca ttaaattagt atttatccag cacataaacg
     1261 aaccaaagcg ttctttccct gagtgtaaat atttaaaatt aaactcaaaa taagtttttt
     1321 tgcttagaaa atttaatggt ttttctatag tgaaaaaaaa gcccttactc gttttcgttc
     1381 gagatactat tttaaataaa tttttatttt caataaaata agaatattca tttactaagt
     1441 tgttatgata aaaaagtaat tttattaaaa tgttttcata attttctagg ttcatttcta
     1501 gctcaattta aaatgatttc attgttgccg taccaatgcg aaatttacca tcaacaaaat
     1561 ttcccttcac tgatacaggt acaccatcaa cttgggtagt tttattttca ggctgcctga
     1621 aagaaagata ggcaacttat tactcatctt acgctagccc tcaataattg aatacgttgc
     1681 actagaacca attatttcta tgaatgtttg ctcaaacata aaaatatagt atttaaagaa
     1741 ttctctattc aaaggaaaat aactttcttt cagtatccat tccgcataac actcatcaag
     1801 aagttcaaaa aaagattgac caccattttc atgaagcaaa gctctatctg catcttccga
     1861 aattaaatcc ataaaagtat ctataaaata cgattcatca acaaccctaa aatgaataac
     1921 atcttcaata aaaattttta tggttttatc cgtatttcta taaccaactg ctataacttc
     1981 tactttatca gagtttattg ctgcattatc aaaatagaaa tagccagttg ggaaatctaa
     2041 gctttttaat ttcataattt atcgtgtatt tccatatctg atttttattt ttcggttatt
     2101 actaattgtt atttcaagtg ttggttctcc accactacta tcattacgag cattaaccgc
     2161 acgcccatca ggtaaattgc ccgttatcgt tccattagga cgtgtttgta catttcgaac
     2221 attcagacta ttaaaatcat ttagtgcgtc attgtaatcc cccactttat catactgaac
     2281 agcgggacct tttgttttac tcctttgagt agtattgctc aaaatgtctt tgatttgtaa
     2341 atcactgcgc gccttaccgg cttctatgcg agctgccaca ctactcgggc tataggggtc
     2401 gctatcaatc gcaggattac caccgctggt tttttgtccg ctagcctgtt tcccaacctg
     2461 ctgtactgta tcgccaattt catctaccgt acctaacgcc ttagtctgca tatggcgaac
     2521 agcaggtttt ttatagccgg tatttttaac ccaatcccat ttggtcgggt taagttctac
     2581 ttttttacct ctccaaaccg tacctgcggc ctctgctacg gcaagggcag tttgggctgt
     2641 tgctgttata ttcggatggg catcagccca ttgtctggcg gaattgatgc cgtcttttac
     2701 cgcaaaagca ctgtcctgta atatgctcgc ggcagcaagt tgtgcttccg ggcttaattt
     2761 tcctgaatta ttaatacctt gtagagtctg ctgcgcagag atgtagcaac tgtattttta
     2821 ccccgtcggg taaaaataca gttgctacgg ctgctgggaa agcagttact attttcccag
     2881 tggctcaaaa ttaattgttt cagcagatat ttttataata atccctacat caataataat
     2941 ttcactataa aattggtatt tatatagctc tatttcgggt ataaacaagg gaaacaagct
     3001 atcttcctta tcctcataat ctacgaaatt atttgtatct aatttaaaat tcaaaatatt
     3061 actgcatttg atatatccga aataattacc ttcaattgta tctgtaaaag ataatgtaag
     3121 ggtatttcca ttatcggaaa agattatttc ataaaggctt acattgtgaa aaatattttt
     3181 tttcatttga caaaatatcc agaatgatga tgattatttg gcaatggatt gccttcataa
     3241 tcttgtcgtt gcggatattt tccctgtaca ccatcggttc gcggcactct ttgggttctt
     3301 ataacctctc catttggacg aatatctatt ttcacgccat caggaccttt ccatgtttca
     3361 tattgagccg catctgaacc agtacgtcta aaaccttgtt cttgcaatgc ttgacgtaat
     3421 tcatctcgcg tagttggtct gatagattta acaactggct tgtttcctcc tgccatctca
     3481 ccgtccaacg tctgcatagg gcgggcagca ggtgttttat agccgttatt tttaacccaa
     3541 tcctatttgg tcgggttaag ttctactttt ttacctctcc aaaccgtacc tgcggcctct
     3601 gctacggcaa gggcagtttg ggctgttgca gttatattcg gatgggcatc agcccattgt
     3661 ctggcggaat tgataccgtc ttttaccgca aaagtactgt cctataatgc gcttgcggca
     3721 gcaagttgtg ctttcggact taaatttcct aaatcattaa taccttgtaa agtttgctgc
     3781 gcagagatgt aggtcggata tttgtatccg acaaaaatat ttgacgcatc tatcgtttcc
     3841 gaaatgccgc tgttggaaaa tgtcggattc gagaatccga cctacggcta tttcgtttaa
     3901 gtcataaata gccattcatg ttccttctga atttgctttt gctatcctta gaaccaatca
     3961 tataaacatt atcatcatta attaatgttc taagtttatg aagcggtcaa atctgtcaaa
     4021 ctaccagaga tgaagcatat cctcctggaa gtaattctgt tcgtgctgtt tccatatctt
     4081 ctttgccatc atcagttact tctctgtcta aataaaatct tacgataagt ttttgtctaa
     4141 tttcatcaaa ttctgccgta attaatctta tattgatatg aatacacggt tcatacctgc
     4201 tattagccag tcaggtgtag ttggatgggg attcattttt aataatatct tcaagcaagt
     4261 gaaatgtaag aaaagcgtag atacttgaag gccggctatc tgattggcaa aaagaaattt
     4321 cggagataaa gaatttccgt ttatattttt cataaattaa atcaacacct tgttttacat
     4381 aaaaactaac ttcctcagga aggatattta taacattctc tccttttact tttatagtta
     4441 atggtttgat ttttatgttt tcatgaatat ctgataggat tatttctaaa aaattatttt
     4501 tatttccagt cattctggct attttatgta tatttccatt gtgttcgatt atcattttat
     4561 tttttccaag gatttccttc tatattgata gtttatctaa agatttcatt taaaatatcc
     4621 tgactaattt cttgtttata taattctaca attttctgtt tctgtgtatt tgtataaaat
     4681 ttttcgttgg tatctgcttg atattttaat tgtctcaata tttccatgat ttcatattct
     4741 gatttttcat catattcttc aatcatgtaa tcaacaaatt gttttagaat atcaaaggct
     4801 tcgtaacttt ccaaaatttt aatatgttta tcggcaaaat tggacatata ccattcgtca
     4861 gtgattggat tagtatttaa atcatctaaa aaaacattta aatcatctaa aaaaacattt
     4921 acatcatcta aaaacatgac taatccttaa acttaaaatc tgttttctca ccggttcttg
     4981 agttttcaat gtaatgtatt tcaattttgt ttacgttttg aacacgctta acccaaccgt
     5041 cttttgctaa ccaaccattt ttagtatcgg acataggagg tattctcgca ggagttttgc
     5101 cctgtgggtt tgccttaact tcttccattg ctaattgttc ctttaaatta ttaggtattg
     5161 tacgaccggt tgatttttca acagattgtg tccctatttt taatgatttg tttcccccag
     5221 ccatttcccc gtctacagtc tgcatagggc gggcagcagg ttttttatag ccggtatttt
     5281 taacccaatc ccatttagtc gggttaagtt ctactttttt accgcgccaa accgtacctg
     5341 cggcctctgc tacggaaagg gcagtttggg ctgttgcagt tatattcgga tgggcatcag
     5401 cccattgtct ggcggaatta atgccgtttt ttaccgcaaa agcactgtcc tgtaataggc
     5461 tcgcggcagc aagttgtgct tccggactta aatttcctaa atcattaata ccttgtagag
     5521 tctgctgagc ggctgtatct gagccggtca gtgtaggttg gattaaaacc cagcattttc
     5581 aacatcttga tgttttacta taaatctatt cttgtgcttc ctttgttaga taaatttcaa
     5641 cacaaaagat acgtatgcat tctctaattt tatttgagat aaataatgca ttgctttgtc
     5701 gatactgtta aataaattac ttccctcgta ataccaatta tcatatgttt gataaaaata
     5761 attatctttc ttgatataaa tatctccccc caatacaagt attctctcat tgataaataa
     5821 atttaatata tctattgcaa tagggacggg taatgcggct tctctaattc caattcctat
     5881 caaagattcc ccttgtctcg aataaatttc aaaaatttca tttaacatgt taatctctat
     5941 ctactatttt tcaatcggta taaaccctat tcacagtttc atcttgtatt tctttatgaa
     6001 tacttaatat attaagatag cttaggaatt ttattttctc agattgaggc aactcatcaa
     6061 aaccaagcca acaataaaaa ttgttatatg attttaaaaa atcaacaatt tttatttttc
     6121 ctaaaaccaa atcttcgact aactgggttg cataaataat atcatttatg attttaacag
     6181 gtaaattatt tgataggtaa ccatgggatt ccgtcatcat tgatttctct aggtgcttat
     6241 ttatctgatt ggtaagcctt ttgcgtaagc ttctttcgcc tccgcaagtg tcatttcatt
     6301 agggccacca tctatattag tttctcctgt attttgccac tgacaaacgt cacagatatc
     6361 atagtccata acttcagttc cgcaaacagg acaatgtagc catatttctc cgtcaatttc
     6421 ccatgtcttt tttgaattct ccatcataat acttctttcc taagtttggt ttaaacattg
     6481 ttttgatttt gaatgttttt ggattggcca aaacatagat attgttattg atatcatagc
     6541 gcactcgtct atgttccgtc tgataaccta atatcttgtc cgatgtctgt tttgataaca
     6601 aatcagacgc cattttttgg tagtcttcaa tggttatatc gccaaattct tttccatgag
     6661 ctttgaaatg tccatttaaa gatttctcag taggaaactt ggactttgtc catctgatgc
     6721 ggtcttgaat tgttcctgtc ggagaaatag atgtatcagg tttaacatat gaggcaggta
     6781 agccgatatt gttatcagag tgtgtagggt gttgctgcgt acttggtttt ggtggcttat
     6841 tcccacctgc catctcccca tctaaagtct gcatagggcg ggcagcaggt gttttatagc
     6901 cggtattttt aacccaatcc catttggtcg ggttaaggtt tactttttta ccgccccaaa
     6961 ccgtagttgc ggcttctgct acggaaaggg cagtttgggc tgttgcagtt atattcggat
     7021 gggcatcagc ccattgtctg gcggaattaa tgccgtcttt taccgcaaaa gcactgtcct
     7081 gtaataggct cgcggcagca agttgtgctt ccggacttaa atttcctaat tcattaatat
     7141 cttgtaaagt ttgctgcgca gagatgtagg tcggatattt gtatccgaca aaaatatttg
     7201 acgcgtctat cgtttccgaa atgccgctgt tgaaaaacgt cggattcgag aatccgacct
     7261 acggctgcta ccaaccatca ttttcagctt cttttagcca agtatcaaaa ttttcaaaag
     7321 tctcgtaata ttttgtatta tctaaattaa ctactataat tttaggatta cctgatttat
     7381 ctgaaatatc aaaacaggca gcccactctt ggtttctagc aaaaggtaat aggtttggaa
     7441 tgccagttat ttcaaaataa atgtcaatta cttctgatat attgctttct gcattttcaa
     7501 accaccaagg aaaagaatat tgttcatcgt agttaatggc atgagtggat tgagctaatt
     7561 ttaaataact ttgcggatat ttaaatcctt taggaagcat tgcttcagga tacggaacaa
     7621 aactattttg ttttatcatt tcctaagtac ctttacatca taaatacttc cgttaggaaa
     7681 tcttttgctc cacccttctg gaactccttt ttgtgtttct tgctttctaa ttcgtagctg
     7741 gatagcttct ccccaagttt gaggctcaat accatcatgc ttaaacctga ttctctcttt
     7801 tgatgataat cggtcattaa aattatgaac attcgcttcc acttttaccc ctgcttcacg
     7861 tgctgccata atacgcgtgt tatccatact agtaggtgct ccatcaggca tattaacaac
     7921 atcaaccctg tcgccaaccc atgatttttt tctttcattt cggctacaat agaatcatag
     7981 ttgtaatttg cactaggctc tgacaaaaat ttgggttgtt aagttgtcat ttttttataa
     8041 attccagcaa actgttgttg taaattatct acttcatgtt ttctaacacc agatacattt
     8101 tcaaattctc ttgaatctac acatacgccc gcatatatct cattcaagat agctcgtaaa
     8161 acaaaaactt catcttgagt tagtgaaata gttgagtttt gattatttat ggatagtata
     8221 ttcatggctt tttcctataa atcatcataa tattttttac ctgatgttgg tctaaatgct
     8281 gtacctccat cgtcagaatt tttatctcgg ataactattg tccctgtttt atcatcccaa
     8341 tacgcagttc taccgcgagg taactctttc atatttgttg gatggctaac aatattttca
     8401 atatgccgag caaaatctgc tggtgaattg atatttaaat ccgtaaattc ttgttgtctt
     8461 atgacatgct tgttataagc atgcccacta ctaatttgtt ctccaattag ttgcgcttgt
     8521 aaagacggtt gtgtggaagc atctgctttg ctgttggacg ttatagattt aggcggtcta
     8581 tt
//
LOCUS       DAXHMX010000088         8578 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHMX010000088 DAXHMX010000000
VERSION     DAXHMX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.088"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..311
                     /locus_tag="ACFM4S_001443"
     CDS             <1..311
                     /locus_tag="ACFM4S_001443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051096.1"
                     /translation="DTTLVLVSVISSFFSSREYIDSRRQVLDTAFSYKSDGRMVLFKS
                     IKQESISFLSEDLKKDWMFHKVKTGEKDGYGSDEMLSVPRVYLEMMSRKTGVPYSSIL
                     "
     gene            complement(758..1120)
                     /locus_tag="ACFM4S_001444"
     CDS             complement(758..1120)
                     /locus_tag="ACFM4S_001444"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFC6051097.1"
                     /translation="MKAARRQLYYTFANRIAEQSRIICIKKLNLLNLQCIQIEVNKLH
                     QKIVAPSPLLHTLKEGVAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNMVCE
                     GFGRHYDTDENASSNIRK"
     gene            1493..2920
                     /locus_tag="ACFM4S_001445"
     CDS             1493..2920
                     /locus_tag="ACFM4S_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6051098.1"
                     /translation="MKISRPLEFTLLQQEYMQHLTERMTQIAKLLNSSANNPDIDIPD
                     FLTEIKDYSEFSVTDENGTYLHWDKFRQIHTEDTRMKWRAVKESRKKIQKPIDFPFEH
                     QFWFCIPDSLQARLHLIDKSCGSSIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLE
                     GAATTRKVAKDMLKSQRKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIAT
                     SNAIENKADPGQFRRDDEIFIADNDGNSLYQPPPHGQVHTLMEAVCTFANNTYDGVEN
                     PFIHPVVQAIILHFLIGYIHPFGDGNGRIARALFYWFMLKNSYWLFEYISISRLLKNA
                     PAQYAKSYLYAETDDSDLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYT
                     EKIGKLNQRQIGILQKAVEESGKIFTALEIANQYGISLNTARSDLSKLGEYRFLVPFK
                     SGNALEYVAPQDLLERLEKKNSLLA"
     gene            3309..3773
                     /locus_tag="ACFM4S_001446"
     CDS             3309..3773
                     /locus_tag="ACFM4S_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051099.1"
                     /translation="MLHIPPTEPDRYVSSIVALNVHSSNGTGDWHSAAALNDDAYPQD
                     FYIYGKDQTRSTLHLLGNVGVIDGTNRFNEMGYYPENIPVWLADHPRACVDYLYTAVL
                     QTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWENLQLWKRKNPIM"
     gene            3900..5189
                     /locus_tag="ACFM4S_001447"
     CDS             3900..5189
                     /locus_tag="ACFM4S_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFC6051100.1"
                     /translation="MMKSILTVSGNRMRKPRITHLDVWANDERIGTLEKGAMYRFTYD
                     NPGSPLLGLHYQDRGKVYISNNMPHIFAQYFPEGFLDAHITSKYAFHDAPFEDNEMLR
                     LAILGRETLGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAISRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IPVAQTSLSEDSSVLLVRRFDVSEQEYFLGMEDFTSLRQYSAEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPTYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(5526..7313)
                     /locus_tag="ACFM4S_001448"
     CDS             complement(5526..7313)
                     /locus_tag="ACFM4S_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6051101.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHDRF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPSYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     FGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWTRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLLGQTLAGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(7368..7826)
                     /locus_tag="ACFM4S_001449"
     CDS             complement(7368..7826)
                     /locus_tag="ACFM4S_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFC6051102.1"
                     /translation="MPIISTLNILSPKLYPSEPYNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPLGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            complement(7992..8327)
                     /locus_tag="ACFM4S_001450"
     CDS             complement(7992..8327)
                     /locus_tag="ACFM4S_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051103.1"
                     /translation="MKQILFKYFMLITCVFGLSSCGYLLSFGQLMFDGSCADWNCSKP
                     TPRWRKAVDDCENRNLEIIKQRFGYKDYAEVIFSEVGQQIQIPYQCRQSNGGYDIRKD
                     KQYGHLFVQ"
     gene            complement(8308..>8578)
                     /locus_tag="ACFM4S_001451"
     CDS             complement(8308..>8578)
                     /locus_tag="ACFM4S_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6051104.1"
                     /translation="NNTTQGACKGLCYSHSSYFAEVPEQYQRDENGNIKMENGKPVET
                     ENWEDYTKKWGIPKTGADGKYINHAIPKLVNPNNPNGEKYETNPF"
BASE COUNT         2493 a         1801 c         1787 g         2497 t
ORIGIN      
        1 cggatacgac cttagttttg gtatccgtaa tatcatcatt tttttcgtca agggagtata
       61 tcgactctag aagacaggta ttagatactg ccttttctta caagagtgat ggtaggatgg
      121 ttctcttcaa gtcaatcaaa caggaaagta tttcttttct gtctgaagat ttgaaaaagg
      181 actggatgtt tcacaaggtt aaaactgggg aaaaagatgg atatggttca gatgaaatgc
      241 tgagcgtacc ccgtgtctat ttggaaatga tgtcgcggaa aacgggagtc ccctactcca
      301 gtattcttta aagtctaagt agaagacttc ttcgtcggtc tttttttgtt gtttggtttg
      361 catggagtaa aactgtacaa ctgttgaagc ataaaaccag caagaaggta aaaaagaaag
      421 aagcagttct ttggatttta gatgttaccg caattagttt cctttcctaa aatttgttta
      481 aattatttgc aatattaata taaacgagat attaatgatg agaaatcaaa aaggcatact
      541 aaatacaatt tgtacaaaat aattgcagta tttaaaaatg ttgattcgta tatgaaaagt
      601 taaaaatgcc aaaatgtaca gttgctaaac tgtaaaactg ctaaagcaac aaaacataaa
      661 aaggaatgca gggatgcaat cactacatct ttttattccg taagcattta tgactttacg
      721 gtcaactgct actctatgtg cctagctttt cagctcccta ttttcgaata ttggacgagg
      781 cattttcatc agtgtcgtaa tgccggccga aaccctcaca aaccatattg gttcttgtgg
      841 cagcaacacc tatccgtttg ttcaagcgac cacaagagta acatgattgg ctggtggcat
      901 tggctttaat ctcttcgata tgaactccat ttttagctac accttctttc agcgtatgca
      961 acagtgggga tggggcaact atcttctggt ggagtttgtt gacttcaatc tgtatgcact
     1021 gtaggttgag cagattcagt tttttgatac agattatccg gctttgttcg gcaattctgt
     1081 tggcgaacgt atagtagagc tgtcgtcttg ccgctttcaa tttgcggtag gtattttacc
     1141 attccatacg tagccgctcg gtacgtttga gccaaatggt atcttcgcca tttgtaccaa
     1201 ttggttttta cattaggctg tgttttagta atttattaat ttcaattatt tgcaagggaa
     1261 aagacaatta ttttccggtt aggaataaac ctatcctgtt gaatacctta aagccaaata
     1321 cgcctatcaa caccatatta aaacacagcc ttttttaata tagtagacac aatctttccc
     1381 tatttatgaa ggtgatcgct tctttctgat tcgtatttta atgcttccta tttttataaa
     1441 aattgactag aatagctcaa ttattaaaaa ttgcgcgatt ttggtattta tcatgaaaat
     1501 ttccagacct ctggaattta ccctgttgca acaggaatat atgcagcatc tgactgaaag
     1561 aatgactcaa attgccaagc tgctgaattc ttccgcaaac aatcctgata tagacattcc
     1621 cgattttctt actgaaatca aagattattc agaattttcc gtgacagatg aaaatggaac
     1681 ctacctgcat tgggacaaat tccgccagat tcacacggaa gatacgcgga tgaaatggcg
     1741 cgccgttaag gaaagccgca aaaaaatcca aaaaccaatt gatttcccgt ttgaacatca
     1801 gttttggttc tgcattcccg actctttgca ggcacggctt catttgattg acaaaagctg
     1861 cggcagttct atcggcacgt ctagcttggg tggcttcggc agaagcgagc aaaacagatt
     1921 cttgctcaag tctctgatta tggaagaagc gattacatcc gcccaactgg aaggtgcggc
     1981 taccacgcgt aaagtggcca aggatatgct caaatcgcag cgtaaaccca aaacaaaaga
     2041 cgaaatcatg atagtgaaca actatcactt gatgaaaaaa gcggtagaat tgaaaaatac
     2101 gccgttaagt gttgaaatga ttttggattt gcaccgcatt gctaccagca acgctattga
     2161 aaacaaggcc gaccccgggc aattcaggcg ggacgacgaa atctttatcg ccgataacga
     2221 cggcaacagc ctgtaccaac caccgccaca cgggcaggtt catacgctga tggaagcggt
     2281 atgcacgttt gccaacaaca cttatgacgg cgtggaaaat ccgtttatcc atccggttgt
     2341 ccaagctatc atcctgcatt tcctgattgg ctacatccat ccgttcggag atggcaacgg
     2401 gcggatagcg cgggctttgt tctattggtt tatgctcaaa aacagctact ggctgtttga
     2461 atacatatca atcagccgtc ttctgaaaaa cgctccggcc caatacgcca aatcctattt
     2521 gtatgcggaa actgacgatt cagatttaac ctatttcatc tattaccaat gcgatattat
     2581 caagcgggcg gttgccgatt tggagcacta catttccgac aaacaaaaac accaacagga
     2641 attcaaagca gcgattgccc aatatactga aaagatagga aagttgaacc aacggcaaat
     2701 tggtatcctg caaaaagcag tggaagaaag cggaaaaatc tttactgcac tagaaattgc
     2761 caaccaatac ggcatctccc tgaatactgc ccgtagcgat ttgagcaaac tgggagaata
     2821 tagattccta gtaccgttca aatcaggaaa tgctttagag tatgttgctc ctcaggattt
     2881 attggaaagg ttagaaaaaa aaaatagttt gctagcctaa aatgcagctt taaccgagtc
     2941 aaaatcaatg cagtccgcac ctttaaaaag aagctgcgga ctgcttgctt tttgctctac
     3001 aaatgatctt tgtagctgat ttaaccaaga ttgtagcaat tttgctttcc aagcaagcag
     3061 ggttagaaaa ttcgatactt ttaattattg gctgtgtttt aatatgatgt tgataggcgt
     3121 gtttggcttt aaggtattca acaggatagg tttattccta accggaaaat aattgtcttt
     3181 tcccttgcaa gtaattgaaa tcaacagatt actaaaacac agccttacat tattggggtg
     3241 actatcctgt aaaatatgtc ctaaaacgtg gaaaccactt ttgctctgct aaattttaag
     3301 gaatctttat gttacatata cccccaacgg aacctgatcg atatgttagc agtattgtag
     3361 ccttaaacgt gcatagttct aacggtacag gcgattggca cagtgcagca gcccttaatg
     3421 atgatgctta cccccaagat ttctatattt atggcaaaga tcaaacacgt agcacgttac
     3481 atttgcttgg taacgttggt gtgattgatg gaacaaaccg cttcaatgag atgggctatt
     3541 atcctgaaaa catcccagtt tggctcgcag atcacccccg tgcttgcgtg gattatcttt
     3601 acacagcagt gttacaaact ggctcaatcg gtcgggtgat tttagatgat tggtttccaa
     3661 gcgatgaaga caagcaatca gtttatgact tacttaacca aatcgaaccg cacttgaata
     3721 ctcaagaatg ggagaattta cagctatgga aacgcaaaaa cccaataatg taatgctaac
     3781 cgaacgagat aaaaggcacg agaagcggta ttactgaatt tcatccgcaa tacgccatgt
     3841 acaattaaat cagcagatgt gtctaaacgt accgctgttc ccgtttgaaa gaaattttca
     3901 tgatgaagtc tatcctcacc gtatccggaa atcgtatgcg taaacccaga atcacccatt
     3961 tggatgtttg ggcaaacgat gaaagaatcg gtactttgga aaagggggca atgtaccgtt
     4021 tcacatatga caaccccggt tctccattgt tgggtttgca ctatcaggat aggggtaaag
     4081 tatatatcag caacaacatg ccgcatatct ttgcacagta ttttccggaa ggctttttgg
     4141 atgcacacat cacaagcaaa tatgcttttc atgatgcgcc ttttgaagac aatgagatgc
     4201 tgcgcttggc aattctgggc agagagactt tgggtcggat acatgtgcgc tgtaatgacc
     4261 cgctttttaa tgaatggatt gacgggttgg agatgaaaaa tccaagaata ttgactgaac
     4321 gggatttgct gggcataaat gcccgacagg tttttcagca atatatggca gaaatcttcc
     4381 atcacggtcg tttcgtcagt gtatccggga tacagcagaa gatgtcctta gatgccatca
     4441 gcagaaatac caagcaaact gcctcatata ttgccaaagg ttttgatgca tccgaatatc
     4501 cttgcttggc tgccaatgaa tttttatgca tgcagaccat caaacaagca ggtattccgg
     4561 ttgcacaaac cagcctgtcg gaagattcat cagtcttatt ggtacgtcgg tttgatgtca
     4621 gtgaacagga gtatttctta ggcatggaag actttaccag tctgcgtcag tattcagcag
     4681 aagataaata taaaggcagt tatgcggcta ttgcacagat tatccgacag atatccggca
     4741 ggcccgatga agatttaatc cattttttta accagcttgc cgccagttgc atattgaaaa
     4801 acggggatgc acaccttaaa aatttttcag tactctatca tgacgaatac gatgtacgtc
     4861 ttgcacctac ctatgatgta ttggatacat caatatacag ggttggaaca caaggaattt
     4921 ttgatgctta tgacgatacg ctggcattaa acctgactaa ccacggtaag aaaacatatc
     4981 cttccaagaa tacattgttg gattttgctg agaaatattg cgatttggga agagaagatg
     5041 catcctttat gatagataca atcgttcaag ctaaagaaca ggttcttgtt aaatactcgg
     5101 atgtattgcg tgagaatgaa tggttggcgc agaagtggca ttttatcccg gatgaaaatg
     5161 aagaaggtct accgtttaca ttccggtagc tgccgggttt gaaccggctg tcagagatgg
     5221 ccggtcgact ttcaccctga aaatcacttc atcttatggt gtttgaaacc gagaaattag
     5281 aagaatcgta ttcggtagga gatatactag gaagattgga aaactggtaa atcattctat
     5341 tgattgagtt ggcggcctat atgtttaatg taggcaaacg agaaggaatc atgtatttca
     5401 tgattccttc ttaaattcct gtgtcaatct aagatcaaaa cacagccatc tcttaccata
     5461 atcatgatag gtgttttatt atgaaaagct atatctataa ttaccgttga tttgactatg
     5521 ccgttttaaa acgtatagcc tacctgaaaa ccgcttgccc atttccttga ttggaaaaat
     5581 tcgggctttt tcaatgcgcg gccggtaaat atatcgtaat gcaggttgcc gccaagcttt
     5641 atctgcccgc gtatcccaat tgctgtgccg gctagagttt ggcccaataa ccatttggcg
     5701 gattgtcctg aaacatgtcc tacatcagcc ccaagataaa gctgatggcc tggtttaaat
     5761 tgccagctca aatcgttgcg ccaataccat ccccgctcgg cagacaaact catttcaccg
     5821 tcgaagccac gtacggtgtg gtgtccgccg atagccagtt tgtcttgcga tgttagcggg
     5881 gttttgttcc attgtgcatg aacggatgtg tcataggcaa atagctgttt accgatttga
     5941 aaaggagtat ttacatcagc cgatgccgtc caaattttca tacgtgacgt gccttctcca
     6001 aaggcttctt caggcgcgcg cagagcatct ttcatgccgg tgccgcgttt atatttcaac
     6061 ttaaaatctg ccgtactgcg gccgatatat tctttgtggg aaagttctgc caaccaaccc
     6121 gcagttttac gccgttgtac agtcagttcg gcatcatcaa tgtaactttt tgtttccctc
     6181 gtccacagtt ttacaccaag ataggttttg cgtttggcat cacgatacaa caggcggttg
     6241 aagccgaaat cagtattgta actttttcca ttatagtcat agacttccga taatccggaa
     6301 accgcctgat ggtaacggta gccattgtga ttgaatgccc atgtccattt accgaaaggg
     6361 gctgaataat gtacggcgta attgtttgat ccgccttctt tgcgatggcc gtcaaaactt
     6421 tcctcgtcgg gcgtaccgcc aattgaacgt ccataattta catagaacat atcactcagt
     6481 ccaaaaggat tgtcggcaga gaaagtgata tttccttggt attttcctgt cgcctcacta
     6541 cccgaattat ccatccccac actcacacgg tagggcagca gacgttgccg ccattgcacc
     6601 acgacatcac tttggtttgg ttctccctct acgggaacga tttggagatc ggcttccgca
     6661 gtcgggagac gtttgagatt ttccagtcct tgttccaaat cacgcagatt caacagatcg
     6721 ttcgagcggg tgggaaattt gttctggaat gctgcaatac gtcctgcatg ggtttgatca
     6781 tcgttagacc gatcgattcg tatggagcgc agatagctcg gtatcagggt taattgaagc
     6841 ttgccactat tcaaatcctg tggcgcagcc aagatacggg tcgtggtata tcccctgccg
     6901 atcaaagcat tttgtgctaa ggacatgatt tgattaatgt tgcccgcatg cagacacttg
     6961 ccagcctgaa aacccgtttc gcgcaaggca cgttttaggg caaactgaaa ccgatcatgg
     7021 tgttcacctt ccaacaccac ttcgttaatg gcaaaacacg gttggctgct gtcatcgccc
     7081 atcaactgat taaccgtttc ccccgtgttt ttttgatgca aacgcacatc gctttcaggc
     7141 tgcatggttt ggcgcaactg ctcttcgcgt tggcgttgct gaatatcttg ctgcatacgg
     7201 atttcggcag ggttggggga agccaacaaa gtagcaggag cgatgatacc tgccaataag
     7261 cagcaccaag acaaaaagcg aatattaggc aaataggata aaggaagttt catggcatgg
     7321 tcgcaaaatc aataaaatca tcaatgggca ttcaatacac gattgcatca agttttaaaa
     7381 gttaaaattc tcaaaccctt atcgctccct ttatgataca aggcgcgtac tgtcgtacta
     7441 ggaaataact ggcgcacagc tgagcgcatt tgatgactgt gtcccaatgg cgcaaaccat
     7501 tgaatcagcc aaatattgtc gccacagttc caatcgctgt tgtcgcgcaa atggcggtca
     7561 gattctaaat aatgcgcctg cgccacttca tcaaaataag cccatgagat ataaccgatt
     7621 ggttgggtac ccttgcaaaa caaagcgaac tgcccgtttt ttaacacagg caatatatac
     7681 gtcatcatct ccacaatagg tacttggcga tgcgtaggcg actgatacca tagccaagtg
     7741 atggcaccga gtgcttcgct ttcgttatat ggctcactgg gatagagctt gggtgagagg
     7801 atgtttaggg ttgagatgat gggcatattt taagttaggg ttggtgttgg ttaaaacaaa
     7861 aaatggtttc agcctgaaat gaaacatttc acgttaaaac atagctttag catacgagac
     7921 tgaattgtag ggggcaataa gcaacgaccc taccattcaa caattctcgt taattgcaca
     7981 ggttacgctg gttattgcac aaacaaatgc ccatactgtt tatctttacg aatatcataa
     8041 ccaccattac tttgcctaca ttgataaggt atttgaattt gttgtccaac ttcactaaaa
     8101 atcacctctg cgtaatcttt atacccaaat ctctgtttaa taatctccaa gtttctattc
     8161 tcacagtcat caactgcctt tctccatctt ggcgtaggtt tagaacaatt ccaatctgca
     8221 caactgccat caaacattaa ctgcccaaat gataataaat aaccacaact gcttaatcca
     8281 aaaacacaag taattagcat aaaatattta aaaaggattt gtttcatatt tttctccatt
     8341 cggattattg ggattgacta attttggaat agcatgattt atatatttac catcagcacc
     8401 tgttttaggg attccccatt tttttgtgta atcttcccag ttttctgttt caacaggttt
     8461 cccattttcc attttaatat tcccattttc atctctttga tactgctcag gcacttccgc
     8521 aaaataactg ctatgcgaat agcacaaccc cttacatgca ccttgtgtgg tgttattg
//
LOCUS       DAXHMX010000089         8499 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHMX010000089 DAXHMX010000000
VERSION     DAXHMX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.089"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            82..1266
                     /gene="coaBC"
                     /locus_tag="ACFM4S_001452"
     CDS             82..1266
                     /gene="coaBC"
                     /locus_tag="ACFM4S_001452"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6051105.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDEELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1333..3489)
                     /locus_tag="ACFM4S_001453"
     CDS             complement(1333..3489)
                     /locus_tag="ACFM4S_001453"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6051106.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3582..3788)
                     /gene="rpoZ"
                     /locus_tag="ACFM4S_001454"
     CDS             complement(3582..3788)
                     /gene="rpoZ"
                     /locus_tag="ACFM4S_001454"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6051107.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3847..4464)
                     /gene="gmk"
                     /locus_tag="ACFM4S_001455"
     CDS             complement(3847..4464)
                     /gene="gmk"
                     /locus_tag="ACFM4S_001455"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6051108.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4592..5191
                     /locus_tag="ACFM4S_001456"
     CDS             4592..5191
                     /locus_tag="ACFM4S_001456"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6051109.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5237..6025)
                     /locus_tag="ACFM4S_001457"
     CDS             complement(5237..6025)
                     /locus_tag="ACFM4S_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6051110.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAVKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6350..7705
                     /gene="yegQ"
                     /locus_tag="ACFM4S_001458"
     CDS             6350..7705
                     /gene="yegQ"
                     /locus_tag="ACFM4S_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6051111.1"
                     /translation="MKAPELLLPAGGLERMRTAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7839..8315
                     /locus_tag="ACFM4S_001459"
     CDS             7839..8315
                     /locus_tag="ACFM4S_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC6051112.1"
                     /translation="MLAVCQSGSAPKNAHTASAGTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2160 a         2308 c         2013 g         2018 t
ORIGIN      
        1 ttgtatccaa acggtaacta atgtatccgt actttgttat agaatggctg ctgtttttct
       61 tcgtaattag aaattgtcaa aatgggtaaa catattcttt taggtgtaac gggcagtatt
      121 gcggcgtata agtcttgcga gttggttcgt ctgctgaaaa aacaggggca ttcggttacg
      181 gtggttatga gccgctcggc aactgaattt gtttctccgc tgacttttca ggctttaagc
      241 ggcaatcctg tcctgaccga cacgcacggc ggcaacggtt caaacggtat ggaacatatc
      301 aacctgaccc ggaatgcgga tgtttttctg attgcgccgg caagtatgaa taccgtggca
      361 aaaatctgta acggcgtggc agataaccta ctgaccagtc tggcagccgc acggaaatgt
      421 ccactcgcca tcgctcccgc gatgaatgtg gaaatgtggc tcaaccctgc caaccaacgg
      481 aatatcgcac aactggtttc agacggcatt actgtctata tgccgggctt aggcgaacag
      541 gcttgcggag aaaatggtat gggaaggatg ccggaacctg ccgaattgct ggatttgctt
      601 ccggacttgt ggacaccgaa aattttaagg ggcaagaaaa tcttgattac cgcaggtgcg
      661 acatttgaag ccattgaccc tgtcaggggc atcacaaata cctccagcgg aaaaatgggg
      721 gtagctttgg cgcgggcgtg ccgtgccgcc ggtgcggaag tcagcctgat tcacggacag
      781 cttcaaaccg cgctgccttt cggcatatcc gatacggttc aagccgtcag cgctgaagat
      841 atgcatcgcg cagtgcatcg tttgattgaa aagcaagatg cttttatttc tgttgccgcc
      901 gtctcagact ataaggttaa aaacaagagt acgcaaaaat tcaaaaaaga taaaaatgcc
      961 aaaccgttat ccatcgaatt ggatgagaac cccgatattt tggcttctat tgcctcattg
     1021 ccaaacccgc cgttctgcat cggttttgcc gctgaaacgg agaatgtaat ggcatatgca
     1081 cgagaaaaac gtattaagaa aaaaataccg gtgatagttg ccaatgatgt ttcaatcgcg
     1141 atgggcaaac cgaccaaccg gattaccatt atcggggacg acgaggaact gtcttttccc
     1201 gaaacaagta aagatgaagc ggcaatgcag attgttgaaa ggcttgccgt atatttgaac
     1261 aaataagcaa ttgaacggat aaaccataaa gcgggttgcc tgttaatcaa aaggcaaccc
     1321 gttttacctg cttcaacttc tgatgacttt gcggatatat ggaatactat gcagattttg
     1381 aataatctga ttcaattgat tcagattctt gactttcaat aagaatttga attcgacaaa
     1441 accttccgtt cccgactggg atttagacgg tgtttcgacc gactcaatgt ccgcaccgga
     1501 atcggaaatc gcttgcgcca ttaatgccag caagccgtgg ctgtcttccg attggacttg
     1561 aagcccgaca cggtagttct gcccgttcat attttcccag tctgcatcca gctgctgttc
     1621 gggatcggac tttaacaacg tcgggcaggt atccctatgg ataatcatgc cttttccctt
     1681 aaccaacagc aaacggatgg aatcgccggg aaccggatgg cagcactctg caaaatgaat
     1741 atgcccgctt tcctgaccat caactttaat ggaactgagc ctgacctcgc tgccgaaatg
     1801 ctcccctgcc aactcggcaa tgtgcatggc gacataaaca ggcagggtat gccccatacc
     1861 gacgttgtac agcacttcct caaatgaagt ctgtttgtcg ttgagatcgg caaggtattt
     1921 ttctttaatg ccgtctgaaa gcaggacatc tttgggcagc aaactggaca gggctttttg
     1981 taagaggctc tctcccaaaa cgaccgcatc gtgccggtta aggtttttaa tatattggcg
     2041 tatggcactg cgcgccctgc ctgacacggc gaaattcaac catgcaggat tgggtttggc
     2101 atgttcggat gtaataattt cgacggaatc gccggttttg agcttcgtac gcaacggcat
     2161 catgatattg ttgatacgtg cggcaacggt tttgtgcccg atatcggtat gcaccgcata
     2221 agcaaaatcg acaggcgttg cccctttggg caaagttagg atttttcctt ttggcgtaag
     2281 gatgtagatt tcgttcggaa acaaatcgac tttgacgtgt tcgagaaact caatggcatt
     2341 ggcactgctt gcctgcaaat ctaagatatt tttcagccac cggtttgtgt gaagcaccgc
     2401 ctgatcgacc gtcttagaat atgatttata gctccaatgt ccggcgatac cgccttcggc
     2461 aacagcatcc atttccttgg tacgtatctg aacttcaatc ggcaagccgt aaggaccgac
     2521 caaagtcgta tgcagacttt gatacccgtt gcttttcgga atggcgatat agtctttgaa
     2581 ccgcccgggc ttgggctgat agagggtgtg caatgcgccg agtgcggcat aacaggctgg
     2641 aatgctgttg acaatgacgc ggaaaccgta aatatccata acctcggcaa agcgcagctt
     2701 tttcgccatc attttctgat ggatgccgta caggtttttt tccctgcctt tgattttggc
     2761 ctctatattc gcgcctacca gccgctggcc gaatgcgcgc aagactttgc cgacaacgtc
     2821 ctgccggttc ttccggctct tgtccatcgc tttttttaaa gtctcgtagc ggttgggatg
     2881 caggttttgg aacgataaat cctgaagctc ttgatatgcg ttattcaaac ctatacggtt
     2941 ggcaatctgt gcatagattt caagggtttc ccttgcaatc cggcggcgtt tgtccgggcg
     3001 catcgaaccg agtgtccgca tattatgcag acggtcggca agtttaacga caatcacgcg
     3061 cacatctttg gtcattgcca aaatcagttt gcggaaactc tccgcctgat gttccgcatg
     3121 atcttcaaat ttgagttttt cgagtttgga cagaccgtcc accatctcgg caatcgtatt
     3181 gccgaacacc gccgccattt ccccttttgt cacgcccgta tcttccaata cgtcgtgcat
     3241 cacgcctgca cacagaccct gtatgtccat atgccaaagg gcgagctgcg tcgcaacggc
     3301 aattggatgc gtaatgtatg gttcgccact tttccgggtt tgtccgtcat gagcgtgaaa
     3361 tgcataggca acggccttct cgagctcaac ctgttcttca ggcttgagat aggaagcggt
     3421 acggaaaagc aaggtgcgtg cttcggcagt taaatggtcg taaggtgcgg tgggttgagg
     3481 gacaggcatt tcagacggct ttcggaacat attttatttt tcgtttcaaa ccgtcggact
     3541 gcacggcggc agaatgtgcc gacgtgcgtt tccggcagaa tttatttatt gcgcgtcaac
     3601 agttccgtac cgatatgccc ggcggcgatt tcccttaagg cggtaacggt cggtttgtta
     3661 ttgcggacat cgtccacaag cggcgtattg ccattttcaa gctggcgggc ccgacgggcc
     3721 gctaccaatg tcaggtcaaa atggttggaa atttttccgg tacagtcttc ggtggtaata
     3781 cgtgccatat tatttgcttt ctttcaaaaa tatttaaaat aggaaactgg gtattttcgc
     3841 ggttttttag gaattttcca acaaatctgc aataaatccc agttgccgcg accttttcag
     3901 acggcaggca ttcacaatat ggcgcaaatc ctcctccgcc cgcgccaagt catcattgac
     3961 cacgacaaaa tcgaacaata cggactgttc gatttcatgc cttgccttcg acagcctcct
     4021 ttggataact tcccgactgt ccgtcccgcg tccgttcaga cgtgcagcaa gcacatcgaa
     4081 agacggcggc aggataaaga tgccgacggc ttccggcagc gcgttgcgaa cctgcgccgc
     4141 gccctgaacg tcgatttcca aaattacgtc atagcctgcc gccgccaacg cattcacgcc
     4201 ctccgcgctt gtgccgtaat agttgccgaa cacgtctgcg tattccaaaa aagcttcctg
     4261 cgcgataagc gactcaaact cttctttaga aacaaaatga taatgtacgc cgtttacttc
     4321 gccttcacgc ggcggacgcg tcgtgtgcga tacggaaacg cgcaaaccgt tatggtttgc
     4381 caacagccgc gacaccagcg tggttttgcc cgtgccggaa gcggccgaaa tgataaagat
     4441 gttgcctttt cgataagcgg acatattttt tacctgtata ttttccagcc gattgtatca
     4501 caacggacac tcggtttcct atttgccgat gcccatattt tgccgctatt gttttgattg
     4561 atttggcaag cggcaggctg acggctacaa tatggcgtta aaaacatcaa acttggaaca
     4621 cgcaatgctg gttcatcccg aagctatgag tgtcggcgcg cttgccgaca aaatccgcaa
     4681 aatcgaaaac tggccgcaaa aaggcatctt attccacgac atcacgcccg tcctgcaaag
     4741 cgcggaatac ttccgacttt tggttgattt attggtttac cgctatatgg atcagaaaat
     4801 cgacatcgtt gccggtttgg acgcgcgcgg cttcattatc ggcgcggcac tcgcctacca
     4861 gctcaacgtc ggtttcgtcc ccatccgcaa aaaaggcaag ctgccttttg aaaccgtatc
     4921 gcaaagctac gcgctcgaat acggggaagc tgcggtggaa atccacaccg atgccgtcaa
     4981 acccggttcg cgcgtgctgc tggtcgatga tttggttgcc acgggcggca caatgcttgc
     5041 cgggctggaa ctgatccgca aactcggcgg agaaattgtc gaagccgccg ccattttgga
     5101 atttaccgac cttcaaggcg gcaagaatat ccgtgcaagc ggcgcgccct tatttaccct
     5161 gcttcaaaac gaaggctgta tgaagggctg aaaaccgacc ctgccgtctg aaaccggcag
     5221 ggttgttatg atgcgttcaa atcacgccca aatcttgcaa gcccctcaac acgccgtctt
     5281 catcaacgcc ggggcaaaca tatttcgccg cctctttcac tgcctgttcc ccgttaccca
     5341 ttgccacacc gaacccgact tcagacagca tttccacatc gttcaaaccg tcgccgaacg
     5401 ccatcacgtc cgccatttcc aaacccaatg cttcaaccac gcttctgatg ccgtctgttt
     5461 tcgacattcc cgcaggcagc agatcgaccg cttcctcgtg ccagcgcacc gttttcaagc
     5521 cttcccgttc cacaatatcc gaccagggcg gcatttcgtt ttcctccgca aacaccagca
     5581 tctgatacac cggtttgctt gaaaaataat ccttatcggc aaaaaaatcg ctggcgatat
     5641 gctgcaaggc gcggcacacg cattccgaca gcgcggacac ggcgatcccc tctccgccga
     5701 caaacgcata atccataccc aagccatcca aatgcgcgca aaccctgccc atcaaaccgg
     5761 catccatcgg tacttcgcgc acagtttttc cgtgcagcag cgcaaactgt ccgtttatcg
     5821 ttaccacggc atccatgccc gtttccgcca tcatgtccct gaccttttcg ggaatcgtcg
     5881 ccaaagaccg ccccgttgcc aacgccgtca atatgccttt gccgcgcaaa gccgccaccg
     5941 ccgttttcac ggaaggacgc aaggtatccg tatatttgcg gtacagcgta tcgtcaatat
     6001 cgaaaaacac gattttagga ttcatcacat tctctctcgc attcaaactg ccgcattgta
     6061 tcccaatcag gcaaatactt gataaatcct tataaatttc ccggcaaaat tgaccgcgaa
     6121 cacgaaaagg cggataatcc gcccatcctc aaaccctttt cagacggcat ttgcagcaat
     6181 gccgtctgaa acatttttac aaagcacaca aatcatgttt caacacacag gacgacacat
     6241 aaagcgtcgc cctatgtgtt gccctgattc ggaaggggtt acgcccctcc caaataaagt
     6301 ctgattctac cgccctaaag ggcggggttt caaccgaaaa ggaaacacga tgaaagcacc
     6361 cgaactctta ttgcccgccg gcggattgga aagaatgcgc accgcctacg actacggcgc
     6421 agatgccgtt tacgccggca gcccgcgtta ttccctgcgc gcccgcaaca acgaatttgc
     6481 caaacttgat gttttagaac aaggcattaa agaagcgcac gagcgcaaca aaaaattctt
     6541 tttgaccgtc aacaccctgc cgcacaattc caaactcaaa acctttgttt ccgatatgga
     6601 gccgctgatt gcgatgaagc ccgacgcgct gattatggcg gatccgggct tgattatgac
     6661 cgtgcgcgaa aaatggccgg aaatgccgat ccacctgtcc gtacaggcga acaccaccaa
     6721 ctactggggc gtgaaattct ggcagaacat cggcgtcgaa cgcattattc tgtcacgcga
     6781 attgagtatg gaagaaatcg ccgaaatccg ccaacaatgc cccgacatcg aactcgaagt
     6841 cttcatccac ggcgcattgt gcatcgccta ctccggccgt tgcctattgt cgggctattt
     6901 caaccaccgc gaccccaacc aaggcacttg caccaactct tgccgttggg attacaaagt
     6961 ccacaacgct acggaaagcg atgcgggcga tgcccagctt ctgcaaggtt ttaactttga
     7021 aaaagcccaa gaagaagcca accaaaactt cgaaggcatc aacggtcaga aacgccatcc
     7081 ctacgccgac aaggttttcc tgattgaaga atccaaccgt ccgggcgaga tgatgccgat
     7141 tatggaagac gaacacggca cctacatcat gaactccaaa gaccttcgtg gcattgaagt
     7201 ggttgagaaa ctcgccaaaa tcggcgtgga cagcctcaaa gtcgaaggcc gcaccaagtc
     7261 gctctattat gtcgcacgcg tcgcccaatc ctaccgcaaa gcgattgacg atgccgtcgc
     7321 aggccgtccg tttgattaca gcctgttgag cgaactcgaa ggccttgcca accgcggcta
     7381 caccagcggc ttcctcgaac gccaccacac tcaggattat caaaactacc tgaccggcca
     7441 ttccaccgcc aaacaaagcc aatacgtcgg acacgttacc gaaatcgaca aaaacggctg
     7501 ggcgaccatc gaagttaaaa accgcttcgc cgtcggcgat tcactcgaaa tcatccaccc
     7561 gaacggcaac caaaccatca aattggaaca aatggcccga aaaggccagc ctgtcgatgt
     7621 cgcacccggc aacggcattc aggtcaaaat ccccgatatg cagggcaaag aaaaagccct
     7681 catcgcacgc gtgttgaacc cctgagccac aatgccgtct gaaccccgtt ttcagacggc
     7741 attttgttga atcttacggt acaatggcaa tgatttccca ccgcaagcaa ggatatccga
     7801 catgaaaaaa aatcctcata ctctccgccg ccgccctgat gctggcagtc tgccaatccg
     7861 gcagcgcacc taaaaacgcg cacacggcat ccgccggcac accggccgga gaatcatgca
     7921 aagtcatccg ggacgacacc gcaggaagcg gcaaaaacat catttaccgt tgctgcaacg
     7981 gtcaggcggc aataaatgca gccattgcag acggcgtatt ggaatcaggg attcccgtca
     8041 gcttcggcgg cgggggcgta ccggttgcaa acggcaggaa tctggttaca cggcaagcag
     8101 caaacggcgt tggcaaatcc gactccgagg cctgcgaaca cgccctaatc aatgccgccc
     8161 gcaaattcca gcagaccgcc ggcaaactcg gcgggcgcgg cgttaccggc ttccacagct
     8221 acttcgacaa gcagccgctg caaggcggac aatacgactg tcaggcaggc tcgttccacg
     8281 tccgtgtggt catgcgcgcc aatgtcgtcc gttaacatat cggcaatcaa aaaattaggc
     8341 tgtgttttag taatctgttg atttcaatta tttgcaaagg aaaagacaat tattttttcg
     8401 gttaggaaga gacctatcct attgaataca tttaagttaa atacgtctat caacactata
     8461 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaag
//
LOCUS       DAXHMX010000090         8408 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHMX010000090 DAXHMX010000000
VERSION     DAXHMX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.090"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..146)
                     /locus_tag="ACFM4S_001460"
                     /pseudo
     CDS             complement(1..146)
                     /locus_tag="ACFM4S_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(308..1240)
                     /gene="cysK"
                     /locus_tag="ACFM4S_001461"
     CDS             complement(308..1240)
                     /gene="cysK"
                     /locus_tag="ACFM4S_001461"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6051113.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1434..2039)
                     /locus_tag="ACFM4S_001462"
     CDS             complement(1434..2039)
                     /locus_tag="ACFM4S_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051114.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="ACFM4S_001463"
     CDS             complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="ACFM4S_001463"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6051115.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            3024..3320
                     /locus_tag="ACFM4S_001464"
     CDS             3024..3320
                     /locus_tag="ACFM4S_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051116.1"
                     /translation="MFRILLLLFPCAILAACARNIPASPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3334..4002
                     /locus_tag="ACFM4S_001465"
     CDS             3334..4002
                     /locus_tag="ACFM4S_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6051117.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="ACFM4S_001466"
     CDS             complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="ACFM4S_001466"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6051118.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6432..7295)
                     /locus_tag="ACFM4S_001467"
     CDS             complement(6432..7295)
                     /locus_tag="ACFM4S_001467"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6051119.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7495..8358
                     /gene="rfbD"
                     /locus_tag="ACFM4S_001468"
     CDS             7495..8358
                     /gene="rfbD"
                     /locus_tag="ACFM4S_001468"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6051120.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2005 a         2421 c         1948 g         2034 t
ORIGIN      
        1 gtggattaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
       61 cggcttcatt gccttgtcct gatttttgtt aatccactat atttgtactg tctgcggctt
      121 cgttgccttg tcctgatttt tgttaatcca ctataaaaaa tatttccctc aagcgatgca
      181 gcgacaccaa cacctgatta aaacacaacc ggcgttttcg gcatatacgc aaaaacgcat
      241 tttccggcac ggcgttacac cgataccgaa aaatgcgtct gaatgtttcg acaatccgat
      301 taaagcatta tgccaaatct gcaaaaagtg gcgtagagag atagcgttcg ccataagaag
      361 gcagcagcac gactatcagc ttgccttcgt tttcaggctg tttggcaagc tgcaacgcac
      421 tccaaaccgc cgcaccggaa gaaataccca ccaaaatgcc ttctttttcc gctattgcgc
      481 gggcggtttc aaaagccgct tcgttcggca ctttggtaat gctgtcgtaa attttggtat
      541 tcaaaacggt cggaataaag cctgcgccga tgccttgaat cgggtgcggg cctttttcgc
      601 cgccgctcaa tacgggtgaa gcttcaggct cgacggcaac cactttaact tcgggtttgt
      661 attttttcaa cacttcgccc acgccggtaa tcgtaccgcc cgtgccgacg ccggcaacga
      721 agacatcgac tttgccgtcc gtatcccgcc aaatttcctc ggcggttgtt ttgcggtgga
      781 cttcggggtt tgcctcattg tcgaactggc gcggcataaa ataagtgtcg ggatgcgcgt
      841 ccaccaagga tttcgctttg gcaatcgcgc ccgccatacc ttcggcggca ggggtcagaa
      901 tcagctccgc accaaacgcg cgcaacagca ttttgcgctc cttactcatg ctttccggca
      961 tggtaatcgc cagcttgtag ccgcgtgcgg cacataccat tgccaaaccg acacccgtat
     1021 tgccgctggt tgcttcgaca atgacggtgt ttttgttgat tttgcccgct ttttcggcac
     1081 cctcgatcat tgcttcggca atgcggtctt tgacgctgct gcctggattg aaaaattcca
     1141 gtttcacggc aacctccgcc ttcaaacctt cggtcagacg gttcagtttg accaaaggcg
     1201 tgttgccgat tagttcggtg atgctgtttg caattttcat cttctgctcc tgaataataa
     1261 ggtttcgaca gtaaggcgca atccccatag gcatcctgtc cgattgcagg ggatttgcgc
     1321 cgaaacataa acaaatttcg gatatggctt tatcctaaac ctatgccgcc taaaaaacca
     1381 atatccaaaa tttctttctt tataactttt tgttccgata cggccgcacc gtattatctt
     1441 ccaatttctg aaagcagcag caccagtacc gagacctgca tcaacgctgc cgtcaacccg
     1501 ccgagtcgca gctgcgagcc gccgaacgtt gacggcggct gccggtctat accgcagcgg
     1561 tggacatagg caaccgccat cagcacaaac tgcaacacga accaataaac cggcttcagc
     1621 gcataaagtt tgagcagtgc cgcaaacaca aacacttgaa ccgttcccga aaagcaataa
     1681 tgcaccaacg cacatattcc gacaaaagca agcgtcatcg atacattact gacggcaaac
     1741 agcccgagca gaggatgttc ggggtctgcc tgccatccgt ttccatccgt tttccggttc
     1801 caatgcccga tgaaaaaaaa tatgctgccc aaagtcaggt aaaaatgggg gatgaacaag
     1861 ggcgcggcgc gggtcattcc ccatatgccg ggcatcagct ttgcccccaa aaccgatatg
     1921 cccagccaca gcataatact cgcccaaaac cattgcaaca ttcctgcgcg gaatatcaga
     1981 aacagaaaaa ccgccgcata cagtaaggca gacaaaaaat ccaaatgcag caaaatcatg
     2041 aatacgccaa ctcgccttca aacaccgctt ccgcagggcc ggtcatcatc acatcgccgc
     2101 cgcaggccca ttcgatatat aaagtcccgc ccggcaaaat cacctctacc gttttccctt
     2161 catccaacaa ccccagacgg atacccgcca ccacagccgc acacgcgccc gtaccgcacg
     2221 cttgggtttc gcccacgccg cgctcgaaca cgcgcaaacg aatcgcggtt cggctgacaa
     2281 tctgcataaa gccgacgttg acgcgttcgg gaaactgcct gtgcggttcg ataagcgaac
     2341 cggtttcgcg caccggcgcg cattctacgt catcgaccac aatcaccgca tgggggttgc
     2401 ccatattgac gcagctgaca ggctgaatgc cggattcgag atgcaccccg taaatacagg
     2461 catcatcccc ctcgcccgat tcggggacaa acggtatttc agacggcata aacctcggtt
     2521 tgcccatatt gaccgtaacc atgccgttat cggacaattt cggaaaaata acgccatttg
     2581 ccgtttcaac acaaatttct ttcttatcgg tcaaaccctt gtctgcaaca aaacgggcaa
     2641 aacaacgcgc tccgttgccg cattgctcga cctcgctacc gtcggcattg aaaatacggt
     2701 aacggaaatc cacgccttcg gtttccgaac gcccgaccac caaaagctgg tcgaagccca
     2761 cgccccggaa gcggtccgcc catgccgcaa tcggcgcgtc ctcgggggta aaatcctgac
     2821 tgaccgcgtc aatcaccata aaatcgttgc ccaaaccgtg cattttggta aatttcaaag
     2881 tgttcatatt gttccgaaat cccgtatcgt ctgttgcttc cgaatccgaa atcccccgca
     2941 ccgaagggaa atgcttccat ctcccgccgc ctgaggatta aaatcagcca ttatactgtt
     3001 tgttacccct tacaagaaac accatgttcc gcatattact gttgcttttc ccttgtgcaa
     3061 tccttgccgc ctgcgcccga aacataccgg caagccccgc cgtttcaaat tgcgcgcatc
     3121 ttcaaaaccg caccatcctg tattttgacg cacacggcaa acccgccgac ggcgcagtaa
     3181 agggcggatt ttacaaaatt atcgaaatac agggtgcaaa ctgcatcctt attcaagact
     3241 ttaacagcac gggcaatgcc ctcggcggat cctatgtgcc ggactgccgc aaatcatccc
     3301 ttttcccgaa aaatatttga cttcaaactg cctatgaacc acgacatcac tttcctcacc
     3361 ctgttcctac tcggtttctt cggcggaacg cactgcatcg gtatgtgcgg cggattaagc
     3421 agcgcgtttg cgctccaact ccccccgcat atcaaccgat tttggctgat cctgctgctt
     3481 aacacaggac gggtaagcag ctatacggca atcggcctga tactcggatt aatcggacag
     3541 gtcggcgttt cactcgacca aacccgcgtc ctgcagaata ttttatacac ggccgccaac
     3601 ctcctgctgc tctttttggg cttatacttg agcggtattt cttccttggc ggcaaaaatc
     3661 gagaaaatcg gcaaaccgat atggcggaac ctgaacccga tactcaaccg gctgttaccc
     3721 ataaaatcca tacccgcctg ccttgcggtc ggaatattat ggggctggct gccgtgcgga
     3781 ctggtttaca gcgcgtcgct ttacgcgctg ggaagcggta gtgcggcaac gggcgggtta
     3841 tatatgcttg cctttgcact gggtacgctg cccaatcttt tggcaatcgg cattttttcc
     3901 ctgcaactga aaaaaatcat gcaaaaccga tatatccgcc tgtgtacggg attatccgta
     3961 tcattatggg cattatggaa acttgccgtc ctgtggctgt aagccccatt cattcccgat
     4021 acccgtaaaa aataccgtct gaaacctgtt cagacggcac tttcacccta agcgttactc
     4081 ggcggcattt tcctcacggg caggcgcgtc cagcagggct ttgatggaca gacggacacg
     4141 gcctctgtcg tccacttcca atgctttcac gttcaccacc tgaccgactt gcaggtagtc
     4201 gccgacattg cgtacgcgct cgtgggcgat ttggctgatg tgtaccaaac cgtctttgcc
     4261 cggcatcacg ctgacaatcg cgccgacgtt gttgtcgagg attttcacca cagtgccttc
     4321 atacactttg cccacttcca cttcggcagt aatctgctcg atgcgttttt tcgccgcatc
     4381 gccggcttct tgagtggtcg cggcaatggt aatcgtaccg tcttcggcaa tattgatttc
     4441 cgtaccggtt tcagcggtaa tcgcacggat ggtttcaccg cccttaccga taacttcgcg
     4501 gattttgtct tggttgattt tcatcgtaaa caaacgcggc gcgtgtgcgg acaactcttg
     4561 cgggcccgca acggcggctt tcatctgatc caagatgtgc agacgcgctt ctttggcctg
     4621 tgccaaagcg atttgcataa tttctttggt aatgccttgg attttgatgt ccatttgcag
     4681 cgcggtaacg ccttcggtcg taccggccac tttaaagtcc atatcgccca agtggtcttc
     4741 gtcgcccaaa atgtcggtca ggacggcaaa tttgttgcct tccagaatca gacccatcgc
     4801 gataccggca acgtgtgctt tcaaaggcac gccggcagac agcaggctca aacagccgcc
     4861 gcagacggaa gccatagagg aagagccgtt agattcggta atttcggaga ccacgcgcat
     4921 ggtgtagctg aaatcttcag gtttcggcaa tacggccaac aatgcacgtt tagccaaacg
     4981 gccgtgaccg atttcacggc gtttcggcgc gcccatgcgg cccacttcgc cggtagagta
     5041 cggcggaaag ttgtagtgca gcataaagcg gtcggtgtat tcgccggaca gcgcgtcgat
     5101 gatttgctcg tcgcgcgaag tacccaaagt cgctacggca agggcttggg tttcgccacg
     5161 ggtaaacaat gcggaaccgt gcgtgcgcgg caatacgccg gtttggatgt tcagcggacg
     5221 gacggtgcgg gtgtcgcggc cgtcgatgcg cggttggccg tccaaaattt ggctgcggac
     5281 gacatcggct tccaagtgtt tgaaaatgcc tttgatttcg ttggctgcca gcgtgtcggt
     5341 ttcttcggta atcaaggctt cttttaccgc actccaagct tcgtccaatt tggcagaacg
     5401 cgcttgtttt tgacggattt tgaacgcttc tttaatggtt tcgccggcaa tcccgcggac
     5461 tttggcaacc agttcctcat tggtttcagg tgctttccaa tcccaaagtt ccggattgac
     5521 ttcgtcggca aattcattga ttgcattgat ggcaacctgc atttgatcgt ggccgtaaac
     5581 caccgcgccc agcatcacgt cttcgggcag gattttggct tcggattcca ccatcaacac
     5641 ggctttggaa gtaccggcga ccaccaagtc caattgcgat ttcgccaatt cggctttagt
     5701 cgggttcaaa acgtacacgc cgtttacata accgacgcgt gccgcgccga tcgggccggc
     5761 aaacggtacg ccgctcaaca ccagcgcggc agacgcgccc aacattgcag gaatatcaga
     5821 atcgatttca ggatcgacgg acacgaccat cgctacgatt tggatgtcgt ggtagaaacc
     5881 ttcagggaac agcggacgaa tcggacggtc gatcagacgg ctggtcagga tttctttttc
     5941 gctttgtttg ccttcgcgtt tgaagaaacc gccgggaatt ttgcctgcgg cgtaagtgcg
     6001 ctcgaggtaa tcgacagtca gagggaagaa gtcttggcct tctttcactt ctttgttggt
     6061 ggtaacggca accaaaacca cggtgtcgcc catagagact ttaacggcag cggcggcttg
     6121 gcgggcaatt tcgccggttt ccaaagtaac ggtgtggttg ccgtattgga aagttttaac
     6181 gtgtttgtcg aacattattg ttcctttcaa aataccgcac tgctaaaaca ctaataatgc
     6241 acactaaaat ccgaatgtgc atagttaggg tttcagaccg tgcggcaggt tgtaaacaag
     6301 ctttcagacg acatttcgga cgctgaaagc aattccaaat tataaaggca accatcatta
     6361 aaatccagta ttgatacaat aaaaaggccg tctgaaccat atttctttca gacggccttc
     6421 aaacttaaaa atcaatcctg agtcagcaaa gtcaatgctt cccgatactt ctcgacagtt
     6481 ttctgaatca catcggcagg cactttcggc gcaggggctt ttttgttcca accgctttgt
     6541 tccagccagt cgcggacgaa ttgtttgtca aaagacggcg gattggtgcc gactttgtat
     6601 tggtcggcag gccaaaaacg gctcgaatcg ggagtcaata cctcatccat cagcgtcagc
     6661 gtaccgtttg tatccaaacc gaattcaaat ttggtatcgc aaatgatgat gccgcgcgat
     6721 ttggcatatt ccgccgcttc tgtataaagc tgaatcgctt tggcgcgcac ttccgccgcc
     6781 aattcttttc cgataatgcg ttcgcattct tcaaagctga tgttttcatc gtgatcgccg
     6841 actgcggctt tggttgaggg cgtaaaaatc acttcaggca gttgttgcgc ttcctgcata
     6901 ccttcaggca gttgaatacc gcaaaccgag ccggtttttt gataatcttt ccaaccgctg
     6961 cctgccagat aaccacgcac aatcgcctct actttcaccg gagtgagctt tttagccacg
     7021 acggcgcgtt tctctaaagc tttggcttcg ttttcaggca aaacatcgta aaccgtttga
     7081 ccggtaaagt ggttgggcat aatatgcacc agttttttaa accaaaaatt ggaaatctgc
     7141 gtcagaatct cccctttgct cggaatcggg tcgtccaaaa tcacatcgaa tgcagacagg
     7201 cggtcggaag cgaccatcag catacgttta tcgtcgattt catataaatc gcgcactttt
     7261 ccaaaataga tctttaccaa accaatctca ctcatttcgc ccccccctga aaatatcttg
     7321 aaaataccga cccgacaccc gacaggtttg aatcacaaac caatattcta gccgaagtcg
     7381 gcgcaaaaca atacccatgg cacaaaaaac caacccgtca accgtcggca aagttttggc
     7441 actataatac cgacagcaag tcctacaata cacttttacc aaaggaaata cctcatgaga
     7501 atcctattga caggctcgaa aagccaactg gcacgctgcc tgcgcgaccg tcttccggaa
     7561 gactgggaaa ccattgcgac ggattccgca tccctagaca ttaccgatgc cgatgccgtc
     7621 tgcaacatgg tcaaaagttt ccaacccgac gccattgtca acacggctgc ctatactgcc
     7681 gtcgacaaag cggaaggcaa tgcggcagcg gcatttgccg tcaatgcttc cgccgtttac
     7741 aaccttgcct tggcagcaca tcgcgcccat gcccgattca tccacatctc aaccgactat
     7801 atctttgacg gtaaagggaa aaggccctat caggaaagcg actttaccaa tccttccaat
     7861 gtatacggac aatccaaaac cgcaggcgag ctgctcgcac tgtctgccaa tcccgacagc
     7921 cttatcctgc ggacttcttg gctgtttagc gaatacgggg acaactttgt ccgcacgatg
     7981 ctgaaccttg cgcgggaacg ttccccgctg tccgccgtcc acaaccaaat cggctgcccg
     8041 acctatgccg gcgatttagc tgccgccatc atccgccttt tgcagcaaac gtcgcccgtg
     8101 cgcggcattt accactacgc cggcggcaaa tccgtatcct ggtatgaatt tgcccaacat
     8161 attttccaag cggcatcgca acagcagaca tccttccccg ttcccgaatt gactgccgtt
     8221 tcagacaagg aatatccgac cgccgccccc aggcccgcat acagcatttt ggactgccgc
     8281 aaaatcgaaa acgacttcgg catcaaaccg tcagactggc aaaaagccct tgcgcaggtc
     8341 gtttccaagc tgctctgatg ccgcccgcct ctccgtttcc gccgtcaagc accgccttgg
     8401 cggttttc
//
LOCUS       DAXHMX010000091         8270 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHMX010000091 DAXHMX010000000
VERSION     DAXHMX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.091"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..338)
                     /locus_tag="ACFM4S_001469"
     CDS             complement(<1..338)
                     /locus_tag="ACFM4S_001469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051121.1"
                     /translation="MQHKPLPYQWGKFWNNTIKPEKQAMFFHSICQCIRFQYVSRIKM
                     YSGLTKIKTRRRSRPPPEKRKKCRPKTFRTAFSRKPSFPQGHKTKTENQKQQPKIRHS
                     HESGNPVRSVS"
     gene            330..1253
                     /gene="lpxC"
                     /locus_tag="ACFM4S_001470"
     CDS             330..1253
                     /gene="lpxC"
                     /locus_tag="ACFM4S_001470"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6051122.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
     gene            2189..3637
                     /gene="gnd"
                     /locus_tag="ACFM4S_001471"
     CDS             2189..3637
                     /gene="gnd"
                     /locus_tag="ACFM4S_001471"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6051123.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPTWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            3700..4971
                     /gene="waaA"
                     /locus_tag="ACFM4S_001472"
     CDS             3700..4971
                     /gene="waaA"
                     /locus_tag="ACFM4S_001472"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6051124.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMTECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACWHALASGAAVQVESADAWREAVEKTLSSKGGGMQMQ
                     VRVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            5012..5461
                     /locus_tag="ACFM4S_001473"
     CDS             5012..5461
                     /locus_tag="ACFM4S_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6051125.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGLVSCEARRE
                     VENAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            5494..6468
                     /locus_tag="ACFM4S_001474"
     CDS             5494..6468
                     /locus_tag="ACFM4S_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6051126.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPKGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            6537..6612
                     /locus_tag="ACFM4S_001475"
     tRNA            6537..6612
                     /locus_tag="ACFM4S_001475"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6570..6572,aa:Lys,seq:ttt)
     gene            6984..8237
                     /gene="murA"
                     /locus_tag="ACFM4S_001476"
     CDS             6984..8237
                     /gene="murA"
                     /locus_tag="ACFM4S_001476"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6051127.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
BASE COUNT         1934 a         2016 c         2292 g         2028 t
ORIGIN      
        1 gaaaccgaac gaactggatt cccactttcg tgggaatgac gaattttagg ttgctgtttt
       61 tggttttctg ttttggtttt atgtccttgt gggaatgacg gtttacgcga aaatgccgtc
      121 cgaaaggttt tcggacggca ttttttgcgt ttttcaggag gggggcggct tcgtcgcctt
      181 gtcttgattt ttgttaatcc actatacatt tttatgcggc ttacgtattg aaaacggata
      241 cattggcaaa tactatggaa aaacatagct tgtttttccg gttttatcgt attattccaa
      301 aacttacccc attgataagg aagcggttta tgctgcaaag aactttggcg aaatccatca
      361 gcgttaccgg agtcgggctg cattcgggcg aacgcgtcgc gctgaccctg caccccgcgc
      421 ctgaaaacag cgggatttcc ttccgccgta ccgatttgga cggcgagatg ggcgaacaaa
      481 tcaagctgac cccttatttg atcaacgata cccgcctttc ctccaccatc gttaccgaca
      541 aaggcgtgcg cgtcggcacg atcgaacaca ttatgtccgc gctgtccgcc tacggtatcg
      601 acaacgcgct gattgagctg aacgcgcccg aaatcccgat tatggacggc tccagcctgc
      661 cgtttattta ccttttgcaa gatgcgggcg tggtcgatca aaaggcgcaa aagcgttttt
      721 tgaaaatcct caagcctgtc gaaatcaaag aagcgggcaa atgggtgcgc tttacgccgt
      781 atgacggctt taaggtaacg ctgaccatcg aattcgacca tccggttttc aaccgcagct
      841 cgcccacttt tgaaattgat ttcgccggca aatcctacat cgacgaaatc gcgcgcgcgc
      901 gcactttcgg ctttatgcac gaagtggaaa tgatgcgcgc ccacaatctg ggtttgggcg
      961 gcaatttgaa caacgctatc gtgattgacg acacggatgt cctgaatcct gaaggcttgc
     1021 gctatcccga tgagtttgtg cgccacaaaa tccttgatgc catcggtgat ttgtatatcg
     1081 tcggacatcc gattgtcggc gcgtttgaag gctacaaatc gggacacgcc atcaacaacg
     1141 cactcttgcg cgcggttttg gcagacgaaa cggcttacga ccgggtggaa tttgccgaca
     1201 gcgatgattt gcccgacgca tttcacgagc tgaacatcag aacttgtgga taacgctttc
     1261 tattttttcc ggtaaattcc taaaacttaa aatttcatca ttcctacaag gacaaaaaac
     1321 caaaacagaa acctaaaatt cgtcattccc gcgaacctac atcccgtcat tcccacgaaa
     1381 gtgggaatct agaatctcgg actttcagat aatctttgaa tattgctgtt attctaaggt
     1441 ctagattccc gcctgcgcgg gaatgacggc ggagcggttt ctgttttttc cgataaattc
     1501 ctaaaactca aaatttcatc attcccactt tcgtgggaat gacgggatgt aagttcgtgg
     1561 gaatgacgcg gtacaggttt ccgttacgta tccccatccg aaagtttact tatacacaag
     1621 caatccaaag ttatctgact gaaatattgt gagatttacc cgcaaatctc attttctcca
     1681 ccacttatac acaaactcgg atgcacactc tgccaatatg cagagaaaaa gacattcgtg
     1741 ccgttaactt actattccca cacgttccaa acacatttcc ctctcaaacc tccacagtat
     1801 tcccgttctt aaaggcgcca gcagccattc ggtaaagaaa agtaggatgc catctgcaca
     1861 acaagcaaac tgtccacaaa aactattctt catgaaaaac aatccacagg tatgcacaaa
     1921 agggaatccg ccttaccgac acccttttaa aacggcaatc tcgatgattt ttaaaataaa
     1981 aaaaagttac ccacagaaaa tgtctcctat caatataatc atcgttttat tatttaaatt
     2041 ttatatttta tttataagcc ttcagggaaa cgcacggtaa agcgtaaagg catcttttca
     2101 aaaaattaaa aagttaagga ttgttaaaca agtgtaaatc aggtagagtt gcggtcgata
     2161 aattaaatct ataacggagc ttcctgcaat gaaaggcgat attggtgtaa tcggtttggc
     2221 ggttatgggg caaaacctga ttttgaatat gaacgattgc ggatttaagg ttgtcgccta
     2281 caaccgaaca atcggtaagg tggacgaatt tttaaacggc gcggctaagg gaacggatat
     2341 tgtcggcgcg tattccctgc aagacttggt cgataagctg gcaaaaccga gaaaaatcat
     2401 gatgatggtt cgcgcaggtt cggtggttga cgactttatc gaacaactgc ttccgttgtt
     2461 ggaagaaggc gacatcatta tcgacggcgg caatgccaat taccccgaca cgacacggcg
     2521 gacacattac cttgccgaaa aagggatttt gtttgtcggc gcgggcgtat ccggcggcga
     2581 agaaggcgcg cggcacggcc cgtctatcat gccgggcgga gatgagcgcg cttgggaagc
     2641 cgtcaaaccg atttttcagg cgattgccgc caaaacgccg cagggcgaac cgtgttgcga
     2701 ctgggtcggc agggacggcg cggggcattt tgtcaaaatg gtgcataacg gcatcgaata
     2761 cggcgatatg cagttgattt gcgaagcgta ccagttcatg aaagacggct tggggctgtc
     2821 ttacgacgaa atgcaccgcg tgtttgccga gtggaacaaa accgagctgg attcttatct
     2881 gattgaaatc acagcggcaa ttttgggcta taaggacgaa ggcggcgaac cgctggtcga
     2941 gaaaatcctc gatacggcag ggcaaaaagg cacgggcaaa tggacgggca tcaatgcttt
     3001 ggatttgggt attccgttga cgctgatttc cgaagcggta ttcgcccgct gcgtttcgtc
     3061 gttcaaagaa cagcgcgtgc agaccggcaa gctattcgcc cgaaccgtta cgcccgtcga
     3121 aggcggcaaa caagaatggg ttgaggcgtt gagacaggcc ctgcttgcat ctaaaatcat
     3181 ttcctacgca caaggcttca tgctgatccg cgaagcgggc gaaagctacg gctgggattt
     3241 ggactacggc aacactgcgc tgctgtggcg cgaggggtgc atcatccgca gcgcattctt
     3301 aagcaatatc cgcgacgcgt atgaaaacaa tcccgatttg gtgttcttgg gtgcggatcc
     3361 gtatttcaaa aatattttgg aaaactgcct gccgacgtgg cgcaaggtgg ttgccaaggc
     3421 ggtcgaatgc ggcatcccta tgccttgcat ggcttcggcg attacgttct tggacggcta
     3481 cacgaccgaa cgtctgcctg ccaacctctt gcaggcacag cgcgactact tcggcgcgca
     3541 cacttacgaa cgcaccgaca aaccgcgcgg cgagtttttc cataccaact ggacgggcaa
     3601 gggcggagat accgcttcga caacctacga tatttgattc cgttgccgaa atgccgtccg
     3661 aaccgttcgg acggcatttg ttttgcagga aatagaaaga tgttccaatg gctttatgat
     3721 gtattgtggc tgcttgcgcc gatatggata cggcgttatt tggacaaacg ctccggaagt
     3781 gccccggcat atcgggcgca tcgggacgag cgtttcggca agccgtatcc gaatcccgtt
     3841 accggcgcgg tttggataca cgccgtttcg gtcggagaaa cgcgtgccgc ccagcccttg
     3901 atacgcgagt tgcggcagcg ttttcccgat gcgccgctgc tgatgacgca gatgaccccg
     3961 acgggacggg aaaccgcgca agttctgttt cctgatgcac aatgccgtta tcttccgtat
     4021 gacaaaaaaa cgtgggtacg gcagtttttg cgcgaacacc gcccgatgtt cggcattttg
     4081 atggaaaccg aaatctggcc caacctgatg acggagtgcc ggaaatcggg cgttccgctg
     4141 tttttggcga atgcgcggct gtcggaaaaa tcgctaaacg gttatctgaa agtccgccgc
     4201 ctgatccgtc ctgccgccgc ttcgctgacg ggttgtctgg cgcagacgga ggcggatgcg
     4261 gcgcggttgg cgaaattggg cgcggcatcc gtgcaggtgt gcggcaatac caaatacgac
     4321 ctgatgccgt cggaacagat gaaaacgctg gcggggcagt ttgaaaaacg catcggagac
     4381 cggcaggttg ccgtgtgcgg cagcacgcgc gtttatcggg gtgaagacga ggcggaaaaa
     4441 ctgctggcgg cgtggcaaca atatcgcggc gatgcgctgc tggtcgtcgt gccgcgccat
     4501 cccgagcatt ttcagacggt atttgaaacg gcaaaacgct tcgggtttaa ggttcagcgg
     4561 cgcagcgacg gtttgccggt cgaacctgat acgcaggtgt ggataggcga cagtatgggc
     4621 gagctgtatg cgtattactt gtgcgccgat gtcgcttttg tcggcggcag tctggtcgat
     4681 tcgggctgtc agaacattat cgaaccgctt tcctgcggcg ttccgacgat attcggcttt
     4741 tcaacctaca atttttccga agcctgctgg cacgccttgg catcgggggc ggcggttcaa
     4801 gtcgaatcgg cggatgcgtg gcgtgaagcc gttgaaaaaa ccttatcgtc caaggggggc
     4861 ggaatgcaga tgcaggtgcg cgtggacggc tttatcgcac aacatcgcgg agcgggcgcg
     4921 agaatcgccg aggcggtgcg ggaagcggta tgcggacatc gggggcgata gtgatacaat
     4981 ccgtatccga gttttccgtt tggagcatcg tatgagtatt tatgccgtcg cgcacatcgt
     5041 tcatctgtat tgcgctattg cctttgtcgg cggcgtgttt tttgaagtgc tggttttgtc
     5101 cgtcctgcat acgggactgg tgtcgtgcga ggcgcggcgc gaagtggaaa atgcaatgtc
     5161 ttaccgcgcc gtcagggtga tgccgtttgt ggtcggactg ctgttcgcca gcggcatcgt
     5221 gatggcggca aaccgctatc tttctatatt gggcgaaccg tttgccactt ccttcggtac
     5281 gatgctgacg ctgaaaatcc tgttggcgtt cagcgtgttg gcgcacttcg ccatcgccgt
     5341 cgtcaaaatg gcgcgttcca cactgaccgt cggctggtcg aaatacatac acaccgtcgt
     5401 ctttacccat atgctgctga ttgtcttttt ggcaaaagcg atgttttata tcagctggta
     5461 acacaattac atttcaaatc aggagattga accatgacag aatatcccgg catcgggtcg
     5521 ccgttgtttt acggcgtttt ttttgcggca gtgctggtca tgattgcctt ggatatgttt
     5581 tcgctgaaga aaaacggcag ccataaagtc ggcatcaaag aagccttggc atggagcggc
     5641 ttgtgggttg ccgtatcctg cctgttcgcg ggctggctgt atttcgaact cgccggcaat
     5701 cccggctacg gcgcggcagt cgcaaaagaa aaagtattgg aattctttac cggctacatt
     5761 ttggaaaaat cgttggcagt cgataatatt ttcgtgttcc tgatgatatt cggctacttc
     5821 aaagtcgcac cgcagtttca gcaccgcgtg ctgctgtacg gcgtattggg cgcattggta
     5881 ttgcgcaccg tcatgatttt cgtcggcgcg gcactggttc ggcagttcga gtggattctg
     5941 tatctgttcg gcgcgttcct gctctatacc ggcatacaca tgatgaagcc caaaggcgat
     6001 gagaaagagg atttggcaaa cagcaggctg ctgaatgccg tcaagaaagt cgttccggtc
     6061 ggcacggaat ttcacggcga gaaatttttt accgtcgaaa acggcaaaaa aatcgccacg
     6121 ccgctgtttt tggtgctggt catgattgaa ttgagcgatg tcgtgtttgc cgtggacagt
     6181 attcccgccg tctttgctgt taccaccgat ccgtttatcg tgctgacctc caatattttc
     6241 gccattttgg gtttgcgggc gatgtatttc ctgctggcgg atgtggcgga acgctttatc
     6301 ttcctgaaat acggcttggc attcgtgttg agttttatcg gtgtgaaaat gctggtaatg
     6361 cattgggtgc atatcccgat ttccgtttcg ctgtcggtcg tgttcggcgc gttgggtgca
     6421 tcgatactga cctcgttaat ttatacgaaa aaacagcctg ataaataaaa tgccgtctca
     6481 acgccgacaa aatggttgac agcatcaggc ttgatgtata gaatttcgag cttgtcgggt
     6541 cgttagctca gccggtagag cagcggactt ttaatccgtt ggtcgagcgt tcgaatcgct
     6601 cacgacccac cagataaaac aaaaaaagct gagttttcaa aactcggctt ttttgtcttc
     6661 cgacaatcct tgttcggaaa gcgcggcatt tcgtcaaatt ctttggtgcg gcttccgtat
     6721 ttttcccgaa aatcccttat aattccgcct ctgcaacctt catcgggtga tgctttatgc
     6781 gttgtcccaa gaattgaaat attatagtgg attaacaaaa accagtacgg tattgcctcg
     6841 ccttagctca aagagaacga ttctctaagg tgctgaagca ccaagtgaat cggttctgta
     6901 ctatctgtac tgtctgcggc ttcgccgcct tgtcccgatt tttgttaatt cactatacaa
     6961 gtacccacca ggaaagaaca aacgtggaca aactgaaaat ctccgcaaac ggcccgctca
     7021 acggggaaat aacggtctcg ggcgcgaaaa acgcggcatt gccgctgatg tgcgcgggtt
     7081 tgctgacatc gggtacgttg cgcctgaaaa atgtgcctat gctggcagat gtgaaaacca
     7141 cgcaaaagct gcttcagggg atgggcgcgc gcgtcctgac cgacaatatc agcgaatttg
     7201 aaatcaacgg cggtacggta aacaatacgt gcgcccctta cgagttggtc cggacgatgc
     7261 gcgcttcgat tttggtgctg ggcccgacgc tggcgcgttt cggcgaggcg caagtcagcc
     7321 tgccgggcgg ctgcgccatc ggttcgcgcc ccgtcgatca gcatttgaaa ggcttggaag
     7381 cgatgggtgc tgagattgtt atcgaacacg gttacgtcaa ggccaaaggc aaactcaaag
     7441 gtacgcgcgt ggcgatggat gtcgttaccg tcggcggcac ggaaaacctg ctgatggcgg
     7501 cgacgctggc ggaaggtacg acggttttgg aaaactgcgc catcgagcct gaagtggtcg
     7561 atttggcgga atgcctggtc aaaatgggcg cgaaaatcag cggcatcggt acgtccacga
     7621 tgattgtgga aggtgtggac gagctgcacg gctgcgaaca cagcgtcgtc cccgaccgga
     7681 ttgaagcggg gacgttcctg tgcgcggtgg cgataaccgg cggcagggtg gttttgcgga
     7741 atgccgcccc gaaaacgatg gaagtggtgt tggacaaact ggttgaggca ggtgcggtga
     7801 ttgaagcggg cgacgattgg atcgccatcg atatgcggca gcgtccgaag gcggtggaca
     7861 tccgcacggt cgtccacccc ggcttcccca cggatatgca ggcgcagttt atggcattga
     7921 atgccgtggc agagggaagc tgccgcgtgg tggaaacgat ttttgaaaac cgctttatgc
     7981 acgtccccga gttgaaccgg atgggggcga acatcacaac cgagggcaat acggcatttg
     8041 tgcagggtgt ggaacagctt tccggcgcgg tcgttaaggc gacggatttg cgtgcgtccg
     8101 caagcctcgt tatcgccggt ttggcggcgc gcggcgaaac cgtggtcgaa cagatttacc
     8161 acttggatcg cggttatgaa aatattgaaa aaaaactcgg cagcgtcggc gcgaaaatcg
     8221 agcgcgtatc cggctgatcg ggttgcgatg ccgtctgaag ccttcagacg
//
LOCUS       DAXHMX010000092         8172 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHMX010000092 DAXHMX010000000
VERSION     DAXHMX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.092"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM4S_001477"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM4S_001477"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6051128.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLTGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM4S_001478"
     CDS             complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM4S_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6051129.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1936..2943
                     /locus_tag="ACFM4S_001479"
     CDS             1936..2943
                     /locus_tag="ACFM4S_001479"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6051130.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            2959..3138
                     /locus_tag="ACFM4S_001480"
     CDS             2959..3138
                     /locus_tag="ACFM4S_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051131.1"
                     /translation="MPSERTFCRSDGIFISFFRLPPLENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            3276..3599
                     /locus_tag="ACFM4S_001481"
     CDS             3276..3599
                     /locus_tag="ACFM4S_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051132.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3686..3967
                     /locus_tag="ACFM4S_001482"
     CDS             3686..3967
                     /locus_tag="ACFM4S_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051133.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4020..4493
                     /locus_tag="ACFM4S_001483"
     CDS             4020..4493
                     /locus_tag="ACFM4S_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6051134.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4747..5082
                     /locus_tag="ACFM4S_001484"
     CDS             4747..5082
                     /locus_tag="ACFM4S_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051135.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            5262..7952
                     /gene="glnE"
                     /locus_tag="ACFM4S_001485"
     CDS             5262..7952
                     /gene="glnE"
                     /locus_tag="ACFM4S_001485"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6051136.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFSYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFIYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRKLHRQIHS
                     EVGKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTRETLEKLAESGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQLLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPEEQTQSNSEWQWVWQDKPDEEERQGRLKEHGFDAETIATR
                     LDQIRHSHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNWLSTATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALADEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIATDCGLIDKTLAEQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSGELLAHYGNVRKLWREVFGEEAATA"
BASE COUNT         2045 a         2075 c         2153 g         1899 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcgtg ttcgccgaac gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttaatca
      781 tacggaggga atcgccgatg gcgtgcgcgc cggtggtgca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga aatcagatta atcagagaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgcc gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aaacccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 cgaaaccgcg cacctcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggtgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tctgtaattt
     1381 gcggcagggg ttcaaacagt ttgccatata agggaaaagc ctctattgcg cggtgcagca
     1441 gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg gcattgatgt agtcgatagc
     1501 cagttggacg gtggtgattt tttcggcatc ttcgtcgggg atttcgcagc cgaatgcttc
     1561 ttccaaagcc ataaccagct ccacggtgtc caaagaatcc gcgcccaagt cgtcttggaa
     1621 agaagattcg tttttcacgt cggcttcgtt tacgcccagt tgttcagcaa caattttttt
     1681 aacttgttgt tcgatgtttg acatatcagt cgttccttta tgccttgcgg caggttgttt
     1741 aagggaaata aatcggtggt attgtaccga cttttaatag agttttctat ctaatgacta
     1801 ttatatcaat atttgccgat ttgtacattt ttgggtgcgg cgggttttgt cgttcaagtt
     1861 tgacctgtgt gccgtatgtt tgacgggatt tcggttaaaa tgacggcatt tccatctgaa
     1921 gcagaaagcc ctgtcatgta tccacttgcc cgccgcatcc tgtttgcact cgatgccgag
     1981 aaagcccacc acttcacgct cgacgcgctc tacacggttt ataaattggg tctgattccc
     2041 gtaaccggca accgtaccaa acctgtaaaa ttgatgggta tggatttgcc caaccctgtc
     2101 ggacttgccg ctggactcga caaaaacggc gaatacatcg acgcattggg cgcgctcggc
     2161 tttggtttca tcgaaatcgg cacggtaacg cccaaaccgc agcccggcaa cccgcagccg
     2221 cgcctcttcc gcgttcccga acaccaaggc atcatcaacc gcatgggttt caacaaccac
     2281 ggtatcgaca ccatgatacg caacatcgaa aaaagtaaat tcagtggcgt attgggcatc
     2341 aacatcggta aaaacgcggt tacacccatc gaaaacgctg ccgatgatta tttaatctgc
     2401 cttgaaaaag cctacgcaca cgcaagttac attaccgtca acatttcctc gcccaacacc
     2461 aaaaacctcc gcgcgctgca aggtggcgac gagttgagcg cattgcttga ggctttgaaa
     2521 aacaaacagg cacagcttgc ctctgtatac gggaaatacg ttccgctcgc cgtcaaaatc
     2581 gcccccgatt tggatgaagc acaaatcgaa gacatcgccc acgttgtcaa atccgtcgaa
     2641 atggacggca tcatcgctac caataccacc atcgacaaat caagtctcgg cagccatccg
     2701 ctcgcaggcg agcagggcgg tttgagcggg cggcccgttc atgaaaaaag taatcgggtg
     2761 ttgaagctgt tggcagacca catagacggc aagctgccga ttatcggcgt aggcggcatt
     2821 atggaaggca gggacgcggc agataaaatc cgcttgggcg caactgccgt ccaggtgtac
     2881 agcggattga tatacaaagg tccggcattg gtcaaagaat gtttgaaggc tttggcgcga
     2941 tgacgcgatc cgcccaaaat gccgtctgaa cgcacgtttt gccgttcaga cggcattttc
     3001 atttcctttt tccgcctgcc gccccttgaa aatcccttac gcgccgccct gtttgaaaca
     3061 aagcaaacgc ccgccgcgaa ccggacaatc ttgatacgca gccaaagcaa gtccggcggt
     3121 aagttgttaa atatataagc aattgataaa aaaaataatt ttatgccgtg gtggacggtt
     3181 tgagaaaacg caaaatatga acggcgttga aattcatgat gtggaaaata aggatacggc
     3241 ggataatcaa aagattttat atttaaggat ttgatatgtt taaggacgaa ctaaatgaat
     3301 ttatccgatt gatatccgat ccggaaagtg aattggatga atggtatttg agtgatttta
     3361 aagatgaaca tatatgggaa atgcaaagtt acgaagcatt ttcatgtttg cgggaagcag
     3421 tcccctattt gtttgcttat ccccgatacg ggtatgaact gttggagata atttccgcgt
     3481 taaaagaaac atctgatacg acagaattat tttatgagcc gggtatcgtg cctttgctga
     3541 ttgatttata taaagaagat agttatctgg taaatatggt caaacggata ttcaaatgat
     3601 gaaaggggcg cgggcgtttg cttggcggaa ggcaggtaac tcaatttgaa ggacgcggaa
     3661 gccgatacgt taaaaaggat gaaaaatgga aaacgggcaa cggaataacc ggtttccttt
     3721 ggaaaaacga attttttatc tggagcattc ggggcggtat ctgatgattt gcgccttatc
     3781 ggattattcc caaaacaaac atactgtcgt tatggcaaat ttcatctatc cggatgaaaa
     3841 aacggattgg cggaatttgg atgatttatt caatgagttg gttttagagg aattgcaggc
     3901 ttcatttatg gattggcatc cgactgttga agaggcaatc agccgtcatt tggaagactt
     3961 ttcgtaacag gcgtcaatcg gaaggggcgg aatgacgggg caggcaatcg gagtaaaaaa
     4021 tgaaccttga tttaaccgcg caaaaagtcc gtctttcttg gaaggatatt ctgtgggggt
     4081 atgggaataa atacttgggt tgggctgatg tggcagctta tgcccgaaaa atgacgcttt
     4141 cagatcatga tgaacgtgtg ttcaaactat ctttaatcaa caaatccaat attcttgaat
     4201 taaagcctgt tctggaagat ttggcttcgg aaatgaggga ttattcccct aaaaattggc
     4261 tgtacgtcct cttaagcgat gtattccata gaaaagaaga atttgaggat cctttggggg
     4321 aagttgaaaa aatttatgca gattttgatt atccggaaga aatagaatca tttgtcaggt
     4381 atatgccgcc caaagacggt tatattcctt ctgcccacac ctatgaagaa aatattgccc
     4441 ggttatattc tcactgggaa cactatttga acaacggcgg agggcagggt taaaaccggc
     4501 aatccgatgc cgtctgaagc attatccggc cttcagacgg caaacggatt agagcggaag
     4561 atttgaaagc gggggcgggc tgcattccgg aagcggcaaa acccgaaccg cccgcatcgt
     4621 tgccgataaa ccgataagga aatcgttttg ccggaccggg tgcaaatgtt tgataacaat
     4681 cctgccgccc ggcaaaaatg ctttaaagaa agcaggattg gttaatttaa aaggaaaaat
     4741 taaaaaatga cggttgataa ggaacaagta gaaaagatga tttattctga aaataaacag
     4801 agtgttattg acggtatgct cggtatgacc ttcagctcgg aggaggatga aatcccctgg
     4861 atttccgaga agttgacgga gttgtcgaaa cataaagact tggatattgc cagattgtca
     4921 ttaacctgtt tcggacatct cgccaggatg catgaaaata tcggcgattg cgataaagtg
     4981 attgctttat tgctctcaaa acaaggtgat ccggattttc aaggttttgc cgaagatgcg
     5041 ttagacgaga tttctttatt tatatttaaa aagcgtccgt aagcgcggat taaacctgtc
     5101 aatccgatgc cgtctgaagc gttatccggc tttcagacgg catcgaaggt tgctatgata
     5161 tagtggcttg actttaaacc ggtacggcat cccctcgccc tgtcctgatt tgaagtgaat
     5221 ccgccatcat cttcctgcca ttttttcaaa cggaatccga aatgtccgac aaccgcctcg
     5281 acaccgcccg ccgccattcc ctcttcctcg cccgccagct cgacaacggc aaactcaagc
     5341 ccgaaatttt cctgccgatg ttggacaagg tgttgaccga agcggatttc caagcctttg
     5401 ccgactggga caaaatccgt gcggaagaaa acgaggaaga attggcgcgg cagttgcgcg
     5461 ggttgcgccg ttatgtggtg tcgcagatta tcgtgcgcga tatcaaccgc atcagcgatt
     5521 tgaacgaagt aacccgcacg attacgctgt ttgccgattt tgctgtgaat accgcattgg
     5581 atttttcata cgcctattat caggatatgt acggcacgcc gatcgggcgt tataccaaat
     5641 cgccgcagca tttgagcgtg gtggcgatgg gcaaggcggg cggctatgag ttgaacgtgt
     5701 cttccgacat cgatttaatt tttatctacc ccgaatcggg cgataccgac ggtaggcgcg
     5761 aacgcggcaa tcaggaattt ttcaccaaag tcgggcagaa actgattgcg ttgctgaatg
     5821 acattaccgc cgacgggcag gtgttccgcg tcgatatgcg gctgcggccg gacggcgatt
     5881 cgggcgcgct ggtattgagc gaaaccgcgc tggagcaata tttgattacg caggggcgcg
     5941 aatgggaacg ctacgcgtgg tgcaaaggcc gcgtggtcac gccgtatccg aacgacatca
     6001 aatcgctggt gcgccccttc gtgttccgca aatatctgga ttacggcgcg tatgaggcga
     6061 tgcgtaagct gcaccgccaa atccacagcg aagtcggcaa aaaagggatg gcggacaaca
     6121 tcaaactcgg cgcgggcggc atccgcgaag tcgaatttat cgcccagatt ttccagatga
     6181 tacgcggcgg gcaaatgcgc gcgctgcaac tgaaaggcac gcgggaaacg ctggaaaaac
     6241 tcgccgaatc gggcattatg ccgtctgaaa acgtcgaaac cctgcttgcc gcctaccgct
     6301 ttttgcgcga tgtcgaacat cgtttgcaat attgggacga ccggcaaacc caaaccctgc
     6361 cggcttcgcc cgaacagcgg caactgcttg ccgaaagtat gggatttgat gattacgccg
     6421 attttttgaa tgctttaaat caacatcgga accaagtcaa tcggttgttc aacgaaattc
     6481 tgagcgaacc cgaagaacaa acgcaaagca acagcgaatg gcaatgggtt tggcaggaca
     6541 agcccgacga agaagaacgg caaggccgtc tgaaagaaca cgggtttgat gccgaaacca
     6601 ttgccacgcg gctcgaccaa atccgccaca gccataaata ccgccacctc tccgcacacg
     6661 cccagccgcg tttcgatgcg gttgtgccgc tgttcgtaca ggcggcggca gcgcaaagca
     6721 acccgaccga tacattgatg cggctgttgg attttctcga aaacatcagc cgccgatccg
     6781 cctatctcgc cttcctcaac gaacatccgc aaaccttggc gcaactggcg cagattatgg
     6841 gccaaagttc ttgggtggcg gcgtatctga acaaatatcc gattttgttg gacgaactca
     6901 tcagcgcgca gcttttggat accgcgtttg attggcaggc gctcgccgcc gccctttcag
     6961 acgacctcaa agcctgcggc ggcgatactg aagcgcaaat ggacaccctg cgccatttcc
     7021 aacacgccca agtcttccgc ctcgccgttc aagacctcgc cggactgtgg acggtagaat
     7081 ccctctccga ccagctctcc gcccttgccg acaccatcct cgccgccgcc ctgctgtgcg
     7141 cgtgggcgga catgcccaaa aaacaccgcg acaccccgca atttgccgtc gtcggctacg
     7201 gcaagctcgg cggcaaagaa ctcggctacg cctccgacct cgacttggtc tatctttacg
     7261 acgatcccca tcccgaagca ggcgacgtgt acagccgcct cgcccgccgc ctgaccaact
     7321 ggctttccac cgccaccggc gcaggcagcc tctacgaaac cgacctgcgc ctgcgcccca
     7381 acggcgacgc aggtttcctc gcgcacagca tcgccgcctt tgaaaaatac cagcgcgaaa
     7441 acgcgtggac gtgggaacac cagtccctta cccgcgcccg cttcatctgc ggcacacccg
     7501 aaattcagac ggcattcgac cgcatccgca ccgaaatcct taccgccgaa cgcgaccaaa
     7561 ccgccttggc agacgaaatc atcgaaatgc gcgaaaaaat gttccccact catcccccag
     7621 ccgacagcaa cgtcaaatac gcacgcggcg gcgtggttga tgtcgaattt atcgtccaat
     7681 atctgatact tgcccacgcc cgaaaatatc cgcaactctt ggacaactac ggcaacatcg
     7741 ccctcttgaa catcgccacc gactgtggcc tcatcgacaa aacccttgca gaacaaagcc
     7801 gcaccgccta ccgcttctac cgccggcagc aacacaatac caaactgcgc gacgcagaaa
     7861 aaaccgaagt aagcggcgaa ttgctggcac attacggcaa tgtcaggaaa ttgtggcggg
     7921 aagtgttcgg cgaagaagcg gcaaccgcct gaataaaaaa tgccgtctga agcctgacaa
     7981 tctgggtttc agacggtatt ttcgtaccgt gccgttttaa ggttgcggca gagctaaagc
     8041 gatttatcgg gaatggctga aacccaaaaa ccggattcct ctttcgcggg aatgacggga
     8101 tttcaggctt ctgtttttgt gggaatgatg ggatttttta tccaagcaaa aatcaaaaca
     8161 aacaaataag aa
//
LOCUS       DAXHMX010000093         8120 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHMX010000093 DAXHMX010000000
VERSION     DAXHMX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.093"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(214..2010)
                     /locus_tag="ACFM4S_001486"
     CDS             complement(214..2010)
                     /locus_tag="ACFM4S_001486"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6051137.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGILSDMVSLTGKRTLAQSLAAKNIRIEHPDGLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPSAEQKSDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2066..2407
                     /locus_tag="ACFM4S_001487"
     CDS             2066..2407
                     /locus_tag="ACFM4S_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051138.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFFGSNTENIVKFEHEVLIVCVGRYLDYVTIRAFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2468..3721
                     /locus_tag="ACFM4S_001488"
     CDS             2468..3721
                     /locus_tag="ACFM4S_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6051139.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3727..3879
                     /locus_tag="ACFM4S_001489"
     CDS             3727..3879
                     /locus_tag="ACFM4S_001489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051140.1"
                     /translation="MPSETANRFQTALSRLWKKMPTSRKICANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            3876..5387
                     /gene="lysS"
                     /locus_tag="ACFM4S_001490"
     CDS             3876..5387
                     /gene="lysS"
                     /locus_tag="ACFM4S_001490"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6051141.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5571..5801)
                     /locus_tag="ACFM4S_001491"
                     /pseudo
     CDS             complement(5571..5801)
                     /locus_tag="ACFM4S_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5805..6683)
                     /locus_tag="ACFM4S_001492"
     CDS             complement(5805..6683)
                     /locus_tag="ACFM4S_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6051142.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEALDLPEGSVILGVVLEVKKRF
                     MSAAQARRRVDSDYRP"
     gene            complement(6713..7840)
                     /gene="fic"
                     /locus_tag="ACFM4S_001493"
     CDS             complement(6713..7840)
                     /gene="fic"
                     /locus_tag="ACFM4S_001493"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6051143.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1786 a         2043 c         2257 g         2034 t
ORIGIN      
        1 cgcgccttag ctcaaagaga acgattctct caggtgctga agcaccgagt gaatcggttc
       61 cgtactattc gtactgtctg cggcttcgcc gccttgtcct gatttttgtt aatccactat
      121 atttgcttgc ggcaagatga aaacggtgcg ggatgtttta ggaaaccaat gccgtctgaa
      181 ggctttcaga cggcattttt tgcgccgtgc tgtttaacgc gccagcggtt cggtgcgttt
      241 gatcagccac gcttttgccg cgccttcggt cagcggctcg aggcggcggc ggacttcggc
      301 gtggtagcgg ttgacccagt cgatttcgcc gtcggtcatg agggcggtgt ccatcaggcg
      361 tgtatcgatg gggcagaggg tcagggtttc aaaacagagg aagctgccga attcggtttc
      421 ttgaggggcg gcgacggctt ggttggcggc aaggttttca atgcggatgc cccattttcc
      481 cgggcggtag agtccgggtt cgatggaggt aaccatgcct tttttcatgg cggtttcggg
      541 cgtggcgggg gcggcgaagg cgatgcgctg cgggccttcg tggacgttga ggaaatagcc
      601 tacgccgtgt ccggtgccgt ggccgtagtc gcattgcgcc tgccacaggg gtttgcggca
      661 aatcgcatca atcagcggcg aggggatgtt ttcggggaat acggcttcgg caagcgcgat
      721 atgggctttg agaacgaggg tgttgtcgct tttttgttcg gcactcggcg tgccgacagg
      781 gacgacgcgg gtgatgtcgg tcgtgccgcc tttgtattgc gcgccggagt cgatgagcaa
      841 aagcccgttg ccgctgatgg tgctgtggct ttcgggcgtt gcgctgtaat gcggcagtgc
      901 gccgttggcg ttgaagcctg cgatggtgtc gaaactcaat gaaatgaagc ctgggcgcac
      961 gctgcggtgg cgataaagca tggtgtccac gtcgatttcg gtcaggctgc cgccgttgcc
     1021 gatgatgtct tcaaactcgg cgaagaaacc gcacaacgcc gcgccgtcgt gttccatcgc
     1081 ttcgcggatg cgggcgatgt cggcttcgga tttgcaggat ttgaacagcg tggatgggtt
     1141 gattccctcg ataaggcgca cgctttcggg caggcgcaca agcgtgctga cggcggtttt
     1201 gttcggttcg ataagcagcg cgccgccgat ttgcgcgagt ttgtcggcaa cttgggcata
     1261 aggttcgacc gtgatgcctg cggtttgcag cgcggcggcg gcttcggcgt tcagacggca
     1321 tcggtcggta aacaggacgg cgttgtcttt gccaatcagc aggaaggaca cgaaaacagg
     1381 gttgaaaggt acgtcgctgc cacgcaggtt ggtcagccag gcgatgtcgt cgagcgagga
     1441 aaccaagtgg taatccgcgc ctttttccgc catcacggcg cgcacgcggg cgagtttttc
     1501 ggcggcggtt tcggagacgt aggcggggtc gtggacgaac acggtttcgg caggaatggc
     1561 cgggcggctt gtccacactt ggtcaagcag gccgtcgggg tgttcgatgc ggatgttttt
     1621 ggcggcgagc gattgcgcta aagtgcgttt gccggtgagc gagaccatat cggaaaggat
     1681 gccgacggcg gcgttttcgg gcaggtttgc cgcgagccat tcgttatacg gcggcacttg
     1741 cccgcttttt tgcagttcga tgccgctgcc cgcaagctgc ttggcggctt gttcccaata
     1801 gcggctgtcc acccatacgc ccgcttcgtc ggcggtaacg acgaatgtgc cgaccgagcc
     1861 ggtaaagccc gacaattcgc gccgcgcctg ccaatgttcg ggcaggtatt cggacaggtg
     1921 ggggtcggcg gaagggatga cgagtgcatc caagccttgc gccttcatgg cttcgcgcaa
     1981 tgcggacagg taattcgata cggtattcat aataattcct ccaatcgggt tttgcggctt
     2041 cagacggcat ggacggaaat ctgaaatgcc gtctgaaaaa cataaccatc ttatcaaatc
     2101 gccagcctgc tgcaaaacgg acgggctgat cggatggtgc gtgtttgcgc gaaaaaagcg
     2161 atgtaaggtt gtttgttgta aaattttagg cataaaatgt cgtttcatgc ctttttttgg
     2221 aagtaatacg gaaaatatag tcaagtttga gcatgaagtt ctaatagtgt gtgttggcag
     2281 gtatttggat tacgttacaa tacgcgcttt catcaaaatt tccctaccgg gcggtatcct
     2341 gtttttgaag acggtaggat ttgggtttgt ttgggggcaa tcccgcccaa attggagcaa
     2401 tagatagggc tgtgtccgaa tatgcggctc tgtgtgttga aacacggtta aaaatacgga
     2461 agactttatg tccgaacaac atatttcgac ttggaaaagt aaaatcaacg cattgggacc
     2521 agggattatg atggcttcgg cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc
     2581 gggcgcgctt tacggctggc agatcgcgct catcatcatc ctgaccaacc tcttcaaata
     2641 cccgtttttc cgcttcagcg cgcattacac gctggacacg ggcaagagcc tgattgaagg
     2701 ttatgccgag aaaagccgcg tttatttgtg ggtattcctg attttgtgca tcctctccgc
     2761 cacgattaac gcgggcgcgg tcgccattgt caccgccgcc atcgtcaaaa tggcgattcc
     2821 ctcgctgatg tttgatgccg gcacggttgc cgccttgatt atggcatcct gcctgattat
     2881 tttggtgagc ggacgttacc gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt
     2941 gagtatcgcc acgcttgccg ccgccggcat cgctatgtcg cgcggtatgc agatgcagcc
     3001 ggactttatc gagccgacac cgtggacgct tgccggtttg ggcttcctga tcgcgctgat
     3061 gggctggatg cctgcgccga ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa
     3121 acaacgcatc aatccttccg aataccgcga cgggattttt gatttcaacg tcggttatat
     3181 cgccagtgcg gttttggctt tggttttcct tgcactgggc gcgtttgtgc aatacggcaa
     3241 cggcgaagca gtgcagatgg cgggcggcaa atatatcggg caattgatca atatgtacgc
     3301 cgttaccatc ggcggctggt cgcgcccgct ggtggcgttt atcgcgtttg cctgcatgta
     3361 cggcacgacg attaccgttg tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct
     3421 gctgcgcgga aaagacaaaa cgggcaacgc cgaattcttt gcctggaata tttgggtggc
     3481 gggcagcggt ttggcggtga ttttttggtt tgacggcgta atggcgaatc tgctcaaatt
     3541 tgcgatgatt gccgcttttg tgtccgcccc tgtgtttgcc tggctgaatt accgtttggt
     3601 caaaggtgat gaaaaacaca aactcacatc aggtatgaat gcccttgcat tggcaggctt
     3661 gatttatctg accggtttta ccgttttgtt cttattgaat ttggcgggaa tgttcaaatg
     3721 ataacgatgc cgtctgaaac cgcaaaccgc tttcagacgg cattgtcgcg tttatggaaa
     3781 aaaatgccga catcgcgtaa aatatgcgca aattttgtga ttcggtcagg tcgtctgaaa
     3841 cagattgcgc ctgatttatt ttttcggaaa accctatgag cgaacaaaac catccgcaaa
     3901 ccgagccgca gttggacgaa aaccaaatca tcgccctgcg ccgcgaaaaa ctgcacaaca
     3961 tccgccaaca gcgcaacgcc tatcccaacg acttcaaacg cgacagcttc gccgccgatt
     4021 tgcacgccca atacggcgaa atcggcaaag aagaactcga tccgcaaggc attcccgtca
     4081 aagtggccgg ccgcatgatg ctgaagcgtc aaatgggcaa ggcgagcttt gccaccattc
     4141 aagacgtgtc cgggcaaatc cagctttatc tgaacaacaa aggcgtgagc caagaagttt
     4201 tggacgactt caaccattgg gatttgggcg acatcgtcgg cgcggaaggc actttgttca
     4261 aaaccaacca cggcgaactg accgtacgcg tgtccgacat ccgcctgttg tccaaatcct
     4321 tgcgtccgct gccggacaaa cacaaaggct tgagcgatca ggaaaccaaa taccgccaac
     4381 gctatgtcga tttgattgcc aacgaagaat cgcgcaatac ctttatcaaa cgcagccaaa
     4441 tcatccaatc cgtgcgtaat tttatggtgg gcgagcatta tctcgaagtc gaaaccccga
     4501 tgatgcaccc gattcccggc ggcgcgacgg caaaaccctt cgttactcat cacaatgcct
     4561 tagatattcc gctttacctg cgtatcgcgc ctgagctgta tttgaaacgc ctggttgtcg
     4621 gcggcttgga acgcgtgttt gaaatcaacc gcagcttccg caacgaaggc atgtccgtgc
     4681 gccacaaccc cgaatttacg atgattgaat tttacgaagc cttctccgac tacgaacgta
     4741 tgatgcagat ggcggaagac atcatccgca acgcatcgcg cacagtaaac ggtacggcaa
     4801 acatcactta caatggcaaa gaagtcgatt tggaaagccc gttcgagcgt ctgaccatcc
     4861 tcgaagccat caaaaaatac aatccgcact acaccgacga gcagttgaac gatgcggaat
     4921 ggctgaaaaa agaaatcgtc aaacacggtg aaagcctgcc gccgtccccg ggcatcggca
     4981 gcctgcaact cgcgctgttt gaaggttgcg ccgagggcaa gctgtggaac ccgactttca
     5041 tcatcgatta cccggtcgaa gtttccccgt tggcgcgcgc ttcggatacc aaacaaggtc
     5101 tgaccgaacg tttcgaattg ttcgttgtcg gccgcgaact ggcaaacggc tattcagaat
     5161 tgaatgaccc tgaagaccaa gccgaacgct tcaaatcgca agtcgcacaa aaagacgcgg
     5221 gcgacgacga agccatgcat tacgatgccg actacatccg cgcgatggag ttcggtctgc
     5281 cgccgacagg cggttgcggc atcggtatcg accgcttagt gatgctgctg accgattcgc
     5341 aaaccatccg cgatgtgatt ctgtttccgc agatgcgccc cgagtaatca taaaaacagt
     5401 tgaaatggca atgccgtctg aacctgattg gattcagacg gcattttgta tggcaggatt
     5461 gatagtgaat taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtacg
     5521 gaaccaattc acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag
     5581 ccaacgctgt accggtttaa atttaattca ctatattcgg tttccaactg accgacccat
     5641 tctgacaagg ccgacaggcg ttgttcggat ttcgccacga acgggttttc ggtatcccac
     5701 gcgtagcctg ccaaaatcga agaaagcata cggctgattt ccgggctgcg gtcgggcgcg
     5761 gacacgacgt tttggaagcg gaaatcgtac caaccgtcca cacatcaggg gcggtaatcc
     5821 gaatcaacac gccttcttgc ctgtgcggcg gacataaacc gttttttgac ctccagcacc
     5881 acacctaaaa tcacgctgcc ttcgggcagg tcgagggctt cagacggcct gcccgtctga
     5941 taaatcagca tcctgcccgc aatatcgata acaaggtgcg cgacgaccag ccgcttgtcg
     6001 gacagttcaa tcagtacgat gtcttcgtcc gcagggcgca tacgcggctc gaccaccagc
     6061 aaatccccct tttcgataac cggcgacatg gacgtgtcgg gcatggcaac ggcaaacgtg
     6121 tactgcgagg gggcgaccac cgaagcaaac aagtcgaaac tgcctgtgag tttgcctgta
     6181 cgggcgaaag ccgccgcctg acggtctgac aacagggggc gtggtgtgag cctcatcgac
     6241 agtgctttgc cgtcggcatc gctgtggttg tgcgtatgcg tatgatggtt gttgatggtc
     6301 gtatcgcctt ggacatcgtt aaggactatg ctgtttgaac ctgccacgga tttggtcatc
     6361 tcattgtagg cttgcgtcgt gatgcgctct tgcttggtca tacctgttac agcctgtttc
     6421 agcatttcta acgctatgtg attcttccta agggtcgcta taccggcttt tgcagcttgc
     6481 tcattaccac aagaggggca accccagcta cttcctatga acatattgga gtacctcgaa
     6541 caggtttggt ttccatgttt agggcagttg attacacacg gcttggctgt accctcaaac
     6601 tcgaccaact taatgccacg atgaccgaaa cgttcctcaa cctcagcagc agcttcttca
     6661 aatgtttttg accttcttgc catattgacc tccggattat tgattatttt ggctatgctt
     6721 taacgaggga ttggaatgag gtaaagctgt tgccctctcc ccgcaatagt tccattaggc
     6781 gcggatgaat gaatagtttg tccctgccga tggcgatttc ttgcagcaca cctatgtctg
     6841 aaagctcttt caggtactta gaggccgtct gccgtttggc tatccctgcc gcttctaggt
     6901 tggcaatgcg tgtatatggc tgctcaaaca gaagatttac cagttcgtgc gtgtagattc
     6961 cttgtgcgtg tgtccgtatg tgttgacgtg tctgctcaaa caggcggcgt atcgcatcta
     7021 ttttcgctac cgtccaatcg gcggtgtcgg ctacgccgtc taagatgtag attatccagc
     7081 tttcccagtc ctgccgttcg gttacgccta aaagcaggcg gtaatagtcc gccctgtttt
     7141 cgataatata gcggctcaaa tacaaaatag gcaaatccaa aagccctttt tcaatcaata
     7201 gcaggctgtt caatatgcgc cccgtccgcc cgttgccgtc cgtaaacgga tggatggctt
     7261 caaattggta atgtgccgcc gccatgatga taagcgggtc taaatcgccg ctttcgtgaa
     7321 taaaccgctc ccaatttgcc agcttgccgc gtatggtttc ttctccttcg ggcggggtat
     7381 agacaacatt tccgctgttg cctcctttta gggctgtgcc gcctgttttg cggatggcca
     7441 tttcgtaggg gtgcttgatg gcgttgcaga ccatgatggc ggtttgtgtg cataaagggc
     7501 ggctcgtcag tgattcatag cctgcaaaca gggcggtgcg gtattgcagg gcttctttcg
     7561 tggcagggtc ttgccgttcc gtatccattt gcagggattg aaacagctta tccgttgtgg
     7621 ttacgatgtt ttcaatttcc gaacttgcac gggcttccat aacaggaagg gtgttaatca
     7681 gcatggcttg attcggtatc agttctgccg cctgctttaa acgggcaagg gatgcacggg
     7741 cggttataca acgtttcagg atggttttgc tttcaatatc ctgttttggc ggcaggggtg
     7801 gtaaatcgtt atagggaata tcgggtttcc agttgctcat atttaaaatt tcggaaaatt
     7861 taaagatgtt tccagtatat gtttacgccg tgtatatatc aaggatatat gtttaaaaat
     7921 ttggcttttg taaatatatg ggcggtaaaa ccgcattatt ttatgttgtt acctatttta
     7981 caccttgtct gcacacgatt tccaaacaac ctaaaaagaa acctgccgaa aggcaggttt
     8041 aaagaggtca ttttaaatgt gaactaaagc ttacaccctc gtagggtatc gtgtatatca
     8101 gacgtactta atatacacta
//
LOCUS       DAXHMX010000094         8020 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHMX010000094 DAXHMX010000000
VERSION     DAXHMX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.094"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(68..814)
                     /locus_tag="ACFM4S_001494"
     CDS             complement(68..814)
                     /locus_tag="ACFM4S_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6051144.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(798..977)
                     /locus_tag="ACFM4S_001495"
     CDS             complement(798..977)
                     /locus_tag="ACFM4S_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051145.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(993..1664)
                     /locus_tag="ACFM4S_001496"
     CDS             complement(993..1664)
                     /locus_tag="ACFM4S_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6051146.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM4S_001497"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM4S_001497"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6051147.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM4S_001498"
     CDS             complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM4S_001498"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6051148.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM4S_001499"
     CDS             complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM4S_001499"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6051149.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM4S_001500"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM4S_001500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6051150.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="ACFM4S_001501"
     CDS             complement(4571..5881)
                     /locus_tag="ACFM4S_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6051151.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM4S_001502"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM4S_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6051152.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="ACFM4S_001503"
     CDS             complement(6056..6355)
                     /locus_tag="ACFM4S_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051153.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="ACFM4S_001504"
     CDS             6706..7902
                     /locus_tag="ACFM4S_001504"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6051154.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1893 a         2400 c         1963 g         1764 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcgctgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcac gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag acgaattttt cggatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacagcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcattgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagccccg cgcggaaaag
     3181 ctgcggcgtt tgtgcctgcc aaaggctcgt ccgctcgaca gtggcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaatgga aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcagc gcgcggcatc
     3361 gtgtaccaga atattgtcat ccgccgatgc caagccgatt tccaacagtt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagttta ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc ggcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atagcggtaa agacgggttt gtaccgcacc gtttcacgaa
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg ggtatcggca gccttggcgg
     5281 ggacggacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac aacaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aggcacgctt tggcaacgcc
     7801 ggcgtgatta ccactgccgg cagcaaagcc gttgccgtgg tcattccgac caacgaggaa
     7861 ttgatgattg cacacgatac cgcccgcttg agcggtctgt aagattcaat ccccacgcga
     7921 actgcctccg aaaacggagg cagttttttt atccggcttt ccatgcttaa acagcaccgc
     7981 ctcttttcag acgttgacgg ttgcagccgc ttacctgaac
//
LOCUS       DAXHMX010000095         8012 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHMX010000095 DAXHMX010000000
VERSION     DAXHMX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.095"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(170..2212)
                     /gene="recG"
                     /locus_tag="ACFM4S_001505"
     CDS             complement(170..2212)
                     /gene="recG"
                     /locus_tag="ACFM4S_001505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6051155.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAIGTHALFSDGVTFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDKLPPGRTPIKTRL
                     VNNVRRAEVEGFVLNTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGELNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2298..2741)
                     /gene="secB"
                     /locus_tag="ACFM4S_001506"
     CDS             complement(2298..2741)
                     /gene="secB"
                     /locus_tag="ACFM4S_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6051156.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2762..3019)
                     /gene="grxC"
                     /locus_tag="ACFM4S_001507"
     CDS             complement(2762..3019)
                     /gene="grxC"
                     /locus_tag="ACFM4S_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6051157.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHTAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3233..4726
                     /gene="rng"
                     /locus_tag="ACFM4S_001508"
     CDS             3233..4726
                     /gene="rng"
                     /locus_tag="ACFM4S_001508"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6051158.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4761..6011)
                     /locus_tag="ACFM4S_001509"
     CDS             complement(4761..6011)
                     /locus_tag="ACFM4S_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6051159.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(6024..6181)
                     /locus_tag="ACFM4S_001510"
                     /pseudo
     CDS             complement(6024..6181)
                     /locus_tag="ACFM4S_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6404..7717)
                     /locus_tag="ACFM4S_001511"
     CDS             complement(6404..7717)
                     /locus_tag="ACFM4S_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6051160.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            complement(7822..>8012)
                     /locus_tag="ACFM4S_001512"
     CDS             complement(7822..>8012)
                     /locus_tag="ACFM4S_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051161.1"
                     /translation="PRVRHAPRSAMIGAVSFESAHRDECPRAVSIPIYRLFRIGCRRE
                     MTDGCRNDGRREAEEMID"
BASE COUNT         1890 a         2361 c         2078 g         1683 t
ORIGIN      
        1 gaatgacggc ggagggcttg ccgttttccc gataagtctc tgcggcttcc cattgccgga
       61 tttccgccca cttgggaata acgagattta aaatcatgaa aatgtgtcaa aaataatgca
      121 aaatcagatt ccgttgcact tttttccaat atttccggcg acatttcgct cacacgccca
      181 aataaccttc cctgccggaa agccatcgtg ccaaatgcgc ttcgacgatt tcggggtttt
      241 gttcaatcag tatcggggcg atttcgcgcg cttgttccaa aaggtgtaag tcttcttcga
      301 gcttggcgaa acgcagcata ggcacgccgc tttgacgcgc gccgagaaat tcgccggggc
      361 cgcggatgtt gaggtcttgg cgggcgattt caaagccgtc ggtgtgttcg tagatgactt
      421 tcagccgcgc tttggcgagt tcgcccaagg gttcggcaaa caggaggacg cacacgcttt
      481 ctgccgcgcc gcgcccgacc cgaccgcgta attggtgcag ctgcgccaag cccatgcgct
      541 cggcgtgttc gatgaccatc agggcggcat tgggcacatc tacgccgact tcgataacgg
      601 tggtggcgac caagacgttc agctcccccg aagaaaaccg tgccatcact tcggcctttt
      661 cggcggcctt catgcgcccg tgtaccagtc cgatattcag ctcgggcaat gccgtctgaa
      721 gccgggcgag ggtttcggcg gcggtttgca gttgcagggt ttcgctttct tcaatcaatg
      781 ggcagaccca atacgcctgc cgcccttttc ggcaagtatt gaggacgaag ccttcgactt
      841 cggcgcggcg gacgttgttg acgaggcgcg ttttaatcgg ggtgcgcccg ggcggcagtt
      901 tgtcgatgac ggacacgtcc aaatcggcga aaaaactcat cgcaagcgtg cgcgggatgg
      961 gcgtggcgga catcatcaac tgatggactt cgcgcccttt gtttttaagg gcgaggcgtt
     1021 gggcaacgcc gaaacggtgc tgttcgtcca cgatggtcag gcccaaattg tgaaacgtca
     1081 cgccgtctga aaacagggcg tgcgtgccga tggcgatttt gacgctgccg tcggcgagtt
     1141 tggctttggc ttcgtctttg gcttttttac gcaaactgcc aaaaaggcgg acaacttcaa
     1201 tgcccaaagg ttcgagccat tgtttaaatt taataaaatg ttgttcggca aggatttcag
     1261 tgggcgccat tacagccacc tgcgcaccgg attcgatggc ggtcagcgcg gacaaggcgg
     1321 cgacaatggt tttgccgctg ccgacatcgc cctgcagcag gcggtgcatc gggtaggttt
     1381 gcgccatatc gcggcagatt tcggaaacaa ctttttcttg cgcatcggtc agggcaaacg
     1441 gcagggcttg gcgcagggct tgggtcaatg tgccgtcgcc gcccaatgcc gccgccgtgc
     1501 cgccgatacg cttctgtcgc gccaagcgca tcgaaagctg ttgcgccaaa agttcatcga
     1561 atttgagccg ttgccatgca ggcagcgtgc cgtctgaaag ctgatgaatc gtgaaactcg
     1621 gcggcggcga atgcaaaaga cgcaggcttt cggcgaggtg tggcagcttc agacggcaca
     1681 gcagggcatc gggcagcgtg tcgtgcagcg gcgtaacgtc caacgccgtc tgaataatac
     1741 ggcgcaaagt gggctggttc aaaccgttta cggtcgggta aaccggcgtg aggctttccg
     1801 ccaaaccgcc gccctcggca tcgcggattt tgggatgaat catctcgtcg ccgtaaaagc
     1861 cgtgtttgat ttcgcccacg gcgcggatgc gtttgccgac cgccgtctgt ttctgatggc
     1921 tggcgtaaaa gtggatgaag cgcagaaaaa ggacgctgcc ggaaccgtcg gcgatttgga
     1981 caatcaactg cttgcgcggt ttgaacgtta cttcctgatg gataacctcg ccctcgacct
     2041 ggcacggcac gccaatcggc gcgtccttaa tcggcataat gtgcgtctcg tcctcgtaac
     2101 gcagcggcag gtgcaacacc aaatcccacg cggtatggag gttgagtttg tcgagcttct
     2161 tggcggaaac atcggtgatt ttgagctgtt tctgggtttc gggcgacatc ataggcagat
     2221 tcctttgaac gcacctattt tatccgaaaa caaaaatacc gtctgaaacg gattcagacg
     2281 gcatcggcag gcaggaatca agccccggcg gcttcggctt cctgctgttg ttggtaaatc
     2341 gcctcaaaat taatcggcgc gagcaggacg ggcgggaagc cggcacgtgt taccgtaccg
     2401 gaaacggctt cgcgcgcgta agggaagagg atgttcggac acgccacgcc cagcagcagc
     2461 tgcacatctt cttcggggat gttttccaaa cggaagatgc cactttgggt tacttcgttc
     2521 aaaaacatcg tgcgctcgtt atccagcttg gcggtaacgg ttacggtaac gtccacgttg
     2581 tagtagccgt cttccagctt ttggctgccg gtggaaacgc gcatatccac ttcaggctcg
     2641 ccctgttcca aaaagatttg cggcgcgtgc ggcacttcca aagacaagtc tttgacatac
     2701 agtcgctcga tgctgaatac gggttgcagt tcttcgctca ttttgttttc ctagttgggg
     2761 gttaagggtt cagcagtccg tccagcccgc cttcctgctg gaggcggtag aggtcggtaa
     2821 atccgccgac gtgcgtttcg ccgatgaaaa tctgcggcac gctgcgctgt cccgaaagct
     2881 gctgcatttc ggcaaaggct tcggggcttg catcgacacg gatttcgtcg atatgtccga
     2941 cacctgccgt gtgcagcagc cttttcgcca tcgcgcagta gggacaaaac ggacctgtgt
     3001 acatggtaac ggtctgcata ttgggtttcc gaaagttttg caatgataat caatataggg
     3061 gcatttcccc tgtttggcaa gtgcggaaca gatgcacgtt caaacggcat gtgcggaatg
     3121 tgtcaaagtt tcttttttaa agtatgatag gcattgtgaa aaatattttt gcacccgcgc
     3181 tgcgcggcgg aacgggtgca aaaatatttt tattacattt tcaggaaaaa ccatgttgtc
     3241 aggactcccc atccccaaag acatcgcgcg cccgcccgaa acgatattgg tcaacatcac
     3301 gccgcaggaa acgcgcgtag cggtgttgga ggaaaacaat atctgcgagc tgcacatcga
     3361 gcgcaacagc gaacacagcc tggtcggcaa tatctatctg ggcgtggtgc gccgcgtgct
     3421 gcccgggatg cagagcgcgt ttatcgacat cggcttggaa cgcgcggcgt ttttacacat
     3481 cgtcgatgtc ctcgaacaac gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct
     3541 gtttgaaggg cagtctgttt tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc
     3601 gcggctttcc acccaaatct cgctggcggg gcgtttcctc gtccatcttc cgcaagaaga
     3661 ccacatcggc gtgtcccaac gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg
     3721 cctcgacaag ctcctgccgg aaaatgcctg ccggggctac atcatccgca ccaacgccga
     3781 aaacgccacc gacgaacagc tccagtccga catcgactac ctgaccaaag tgtgggaaca
     3841 catccaagaa caggcgaaaa tccggccgcc ggaaaccctg ctttatcagg atttgccctt
     3901 gagtttgcgc gtattgcgcg atatggtcgg ctgcgacacg caaaaaatcc tcgtcgattc
     3961 caccgtaaac cacgggcgca tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg
     4021 caggatagaa ttgttcaaag gcgaacgccc gctgtttgaa acccacaacg tcgaacagga
     4081 aatcagccgc gccctgcaac cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga
     4141 atccaccgaa gccatgacca cgatagacgt gaacaccggc ggcttcgtcg gcgcacgcaa
     4201 cttcgacgaa accatcttcc gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt
     4261 gaggctacgc aacctcggcg gcatcatcat catcgacttc atcgatatgg cgcaggaaag
     4321 ccaccgcgaa gccgtgttgc aggagcttgc caaagccctc gccttcgacc gtacccgcgt
     4381 taccctgcac ggttttacca gcctggggct ggtcgagctg acgcgcaaac gctcgcgcga
     4441 aaacttaaac caagtcctct gcgaaccctg cccttcctgc caaggcagag gccgtctgaa
     4501 aacgccgcaa accgtgtgct acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta
     4561 cgatgccgaa agtttccgca tcctcgccgc ccccaacgtc atcgatttgt ttttggacga
     4621 agaatcgcaa tccttggcaa tgctgataga tttcatcggc aaaccgattt ctctggcggt
     4681 cgaaaccgct tacacgcagg aacaatacga tattgtgctg atgtaaaaaa tgccgtctga
     4741 agccttcaga cggcatctgt ctatttcagg gtttccttgt ccaacaaggc gcgtatcagc
     4801 agaccgtgtc cgaacctgcg gctgtcggtc agttccaaat atccgccgta ttttttggct
     4861 agcgtgtcgg caatcgacag ccccagcccc gtcccctgct gctccgttcc caaaatacgg
     4921 taaaacggat cgaggacgcg gacgcgttcg gattccggaa tgccgttccc gttatcttcc
     4981 acccacacgg caagatattt cccttcgtct gtgaaaccca aatctatcct gccttcggac
     5041 ggggtataac gtattgcgtt atcaacaaac gttttaacca aggtatagat ttcagtttca
     5101 tcggcaaata tttcgacatc acctccgacc gccacaccaa tgtcctgacg tttttccaag
     5161 gcaagcggca tcagttcctg caacacttgg cggaaacggc tttgcagacc gaatgtcgtt
     5221 ttcgccaaag gggtttcgtc cgactgcgaa cgcgccagtg ccaaaagctg ctcgagcagg
     5281 tgtttgttgc gccgtatgct ttgctgcaaa acggcagact gccgcgccgc atcgggtggc
     5341 agagacatat tgttgagccg ttccgcctga agggaaaggg ctgtcatcgg cgtacgcaat
     5401 tcgtgtgccg cgtcggcgac aaaccgctga cggtggcgga tgtcttcatc cgcacgtttc
     5461 aaaagcaagt tgatggcggt tacgaaccct ctgatttcac tgggaatatt gtccacactc
     5521 aaggcagaca ggtcattgat tcggcgttgt tcgagacttt gcgacaattt gcggacgggg
     5581 cgcatggctt tgtgcgtaat ccacacggtc agcagtatca tcagcggcag tgccgccagc
     5641 aggggcaaca cgctttgccg tgccgcatcc gccgccaaat cttcccggta ttcgctttcc
     5701 tgcatcacgg caatccgccc ccgctccgtc gtgcggatat agacgcggta ataatcgtcg
     5761 tccccgtccg cctgaagcgt gtgcagaccg tccgccagat gcgcaggcag gctgacaaca
     5821 gggtcttcct gctgcggcat ctgtaccaaa atacgcgtat cgccgtcgcc ctcgggcaaa
     5881 gtttcgggtt tggaatcggg ggcgacgtac aatgccgcct gacggagcag gtcgtcctgc
     5941 aacgcttccg tttcgtggaa ggtttcgtag taggaaaaca tacctgcaag cattgccagc
     6001 ggaacaaaca tccaaaccgt ccgccccggc aaacccaaat ccagcagcat caagtcataa
     6061 ggctgggcag cggcagccgc gcgccgtttt tgacccaatc caccgcatag ccgccgtctt
     6121 tcaaacttgc cgacaccgcc tccgcaatca tcgcatcgtc ttccaccagc aaaacacgca
     6181 tcaactttcc ctttcagaca aaccgtgcct attctaacac cccaaaatta gccgcaattt
     6241 agcggccttt acgcttgccg gtatttttca aaactgcagc acaaaaaaac cgcgccggca
     6301 actgccttca gacggcattg gggcgcgatt gcaacacacg ggcaggcagg cagagcctgc
     6361 gacagaccac aggaacgatt cgggcttcag acggcttcgc cgtttacggc agaggcacga
     6421 ttcctgccgc tatcgaactg gccaatatcg ccagcgacaa accccacgcc caaaaaaacg
     6481 aataacggat gtgtttgccc atcgacaatt tcgccaaacc caagcccatc cacaaagccg
     6541 gcgaaagcgg cgtaacaaaa gtgccgacga tactgccgat caacatcgca taacctgctg
     6601 cttcgggcgc cacgcccgcc tgcgaggtaa tctgctccac aatcggaaac agtccgaaat
     6661 aataagcgtc cgtactcaaa accaactcaa gcggaatgcc caacacaccg atggcaatat
     6721 gcagataagg cagcagcgcg tctggcagga tatgcacaat gtctttggaa atcgcgtcca
     6781 acatccccgc acccttcaaa atccccaaaa acgtacctgc cgccaaaata atggacgcca
     6841 tcatcaccgc gccgccggcg tgggcataaa tccgctccat ctgttcctgc gggctgcggt
     6901 aattcaaaag caacgccgcc gttgcagcca gcataaatac ataacccggc gggaagatgc
     6961 ccgaaaaaag caggctcatc gccgccaaaa acagcaggac attccaccaa aacagtttcg
     7021 gacgcgccaa tttttgttct tcttccgaca aaggcaccgg ctttatcaaa tccgccacgg
     7081 cgggcaacgc gcccaactcc cggacaatcc gccttttttc acgcacaccc aaaagcaggg
     7141 acagcgcaag gataaacact acaccgataa tttgcaccgt caacaaaggt ttatacaatt
     7201 cgcccacatc tgcgcccaac acgcttgcaa cccgcccagt cggcccgccc cacggcagaa
     7261 ggttaatcaa tcccgcactg gaagtcagca gcaaaaacag caggtaagga ttcatatgca
     7321 gacgcttgta aagcggcaaa agggcgggga cgaccaataa aaacgtcgtc gcacccgccc
     7381 cgtccaactg cgccaccacc gacaccaaga ccgtccccac actcactgcc acgatattac
     7441 cccgagtcag cttaatcaaa ccgcctatca tcggacggaa cagccccaca tcgttcatga
     7501 ttccaaaaaa caaaatggaa aacataaaca taatcacaat ctgcatcacc gatttggtgc
     7561 cgcccgaata aaattctttt aattgggata catcaaaccc cgccagcaac gccccaaaca
     7621 gcggcaccaa gattaatgcg atgatgggcg acactttttc cgtcagcagc aaccatacga
     7681 tgaccccgat aatcagcagt ccgataaacg tcagcatcat ttctccttta ttttatttta
     7741 aacagaaaac cgaccgtgca ggcaaaaccg tccacagacg cggaataagc cctgcattct
     7801 acttttttat tttaaaataa gtcaatcaat catttcctcc gcctcccgtc tgccgtcatt
     7861 cctgcatccg tccgtcattt cacggcggca accgatacgg aacaaccggt aaatcggtat
     7921 cgagacggcg cgggggcatt catcccggtg cgccgattca aacgaaaccg cccctatcat
     7981 tgcggagcgc ggggcgtgcc gtacacgcgg ga
//
LOCUS       DAXHMX010000096         7633 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHMX010000096 DAXHMX010000000
VERSION     DAXHMX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.096"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(210..1595)
                     /locus_tag="ACFM4S_001513"
     CDS             complement(210..1595)
                     /locus_tag="ACFM4S_001513"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC6051162.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGIVLKEQVYYSTGGGFVVTGEDFERQSETEK
                     TVPYPYNSCAELLDRCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(1694..4084)
                     /gene="gyrB"
                     /locus_tag="ACFM4S_001514"
     CDS             complement(1694..4084)
                     /gene="gyrB"
                     /locus_tag="ACFM4S_001514"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6051163.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSSEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRIIDELVLHAMLHASPVDLSTAESA
                     DRSVAELNGLLDEKEVALERIEGHEGHQFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGKGAKLYKGDNEYDIDSFEGALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            4354..5226
                     /locus_tag="ACFM4S_001515"
     CDS             4354..5226
                     /locus_tag="ACFM4S_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6051164.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAVVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            5314..7350
                     /locus_tag="ACFM4S_001516"
     CDS             5314..7350
                     /locus_tag="ACFM4S_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6051165.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
BASE COUNT         1615 a         2147 c         2122 g         1749 t
ORIGIN      
        1 tcttgccgga tgctgcaaac aacgccggtt tgcattcctg atggcggtgg ttttcttaga
       61 cgaacgcccg aacacgcagg aatggatagg cttggggctg gttacggcgg gcgtgttgac
      121 gctggcactg aaacggtaaa gccgcaagaa ataaatgaaa tgccgtctaa aaaactgttt
      181 tcagacggca ttttcgtttc tgtccatcct cagcactcga ccacgcgcac ggatacgggg
      241 acggcttttt tccggagcgt ggcggcgagt ccggcaagcg aggtttcttt gtaggtcgcc
      301 ttcatatccc ggccggtttg cagcatggtt cggatgactt cgtcgagcga gacttttttg
      361 tccgtgccgt cttctaaaag cgcgagcgtg ccgagtttga gggctttttc ggcggcgatg
      421 ccgttgcgct cgatgcaggg gatttgcacc agtccgccga cggggtcgca agtcagcccc
      481 aaatggtgtt ccatcgccat ttcggcggcg ttttccactt gtttgggcgt gccgccgatg
      541 acttcggcgt atgcgcccgc cgccatcgaa cacgctacgc cgacttcgcc ctgacagccg
      601 acatccgcac cggaaatgga ggcgttggtc ttgtagagga tgccgattgc gcctgcggtg
      661 agcaggaagt tttcgacgcg ttcctgtgtg gcgtgcggat tgaacttgcg gaaatagtgc
      721 aatacggcgg gaatgatgcc tgccgcgccg ttggtcggtg cggtaacgac gcgtccgccg
      781 gcggcgtttt cttcgttgac cgccatggcg tacaccatcg gccagagctg ggtgttgacg
      841 atttcggttt cgcgcaggac tttgagcttg gcggcaagct gcggggcgcg gcggcggacg
      901 ttcaatccgc cgggcagttc gccgtccgca cccaagccgc gtttgatgca gccttccata
      961 acctcggcaa cggcagcggc gcggcggcgg atttcggctt cgccgcatcc ggcaagcgcg
     1021 gcttcgtttg ccaacacgac ttcggagatg tcgagccggt tcagacggca tcggtcaagc
     1081 agctcggcac aactgttgta aggataagga acagtttttt ccgtttccga ctgccgctcg
     1141 aaatcttctc cggtaacgac aaacccgccg ccggtcgagt aataaacctg ttctttcaag
     1201 acgatgccgt ctgaagcata ggcggtaaat cgcaggccgt tggggtgttt gggcagcact
     1261 tgattgccgc gtatgttcag gtcgcggtcg gggatgaagc ggatttcttg cccgttgagc
     1321 cggaggatgt gctgcgtgcg gatgcgttcg aggcgttcgg gaatgccggc aagcgggatg
     1381 tcgtgcggca ggctgccttc caaaccgagc atcagcgcgt caaatgtacc gtgtccgtat
     1441 ccggtcagtg cgagcgagcc gtaaatgtcg atgacgatgc gaacagcctg tgcatccaaa
     1501 cctgccgcaa aggcggcggc tgccttcatc gggccgaccg tatgcgaact ggaaggcccg
     1561 ataccgattt tgaaaatatc aaaaatgctg accatggttt tgctccgaaa ggcggtttca
     1621 gacggcatgg cttccgtttg acaaaccaaa aggagacgcg gcacgatgcc cgtctccttt
     1681 tttaaaacgg cacttatgcg tcgatatttt gggcaatcag cgcgttgttt tcgataaagg
     1741 cgcggcgcgg ttcgacctcg tcgcccatca gggtaacgaa tacttcgtcg gcggcaatgg
     1801 catcttcgat gcgcactttc aacaggcggc gcacggcggg atccatcgtg gtttcccaca
     1861 gctgttcggg gttcatctcg cccaagcctt tgtatcgttg gatggacata cctttttggg
     1921 caacgctcat caagatgtcc aaagcgcctt caaagctgtc gatgtcgtat tcgttgtcgc
     1981 ctttgtagag cttcgcccct ttgccgacca tgcctttaag tgcggcggcg gtttgggtaa
     2041 gggtttgata ggctttgctg ttgaggaact taggctcgag gtagctcacc accacattgc
     2101 cgtgcagctt gcgtgtgatt ttgatgaact ggtgtccttc gtggccttcg atacgttcga
     2161 gcgcgacttc tttttcgtcc aagaggccgt tcaattcggc aacggagcgg tctgcgcttt
     2221 ctgctgtgct gagatcgacc ggcgaagcat gtagcatggc atgcaggacg agttcgtcaa
     2281 tgatgcggct ttcttgctcg atgacggttt tggccaacag gaattgtttg gcggtattgg
     2341 caagctcttc gccttcgatg gtgcggctgt ctgaaacgat tttggctttt tccaaggcta
     2401 aaccgagcag ccattggtct ttttccaatt cgtctttgag atatcgttct tgtttgccgt
     2461 atttggcttt gtataaaggc ggctgggcaa tgtagatgta gccgcgttcg accagctcgg
     2521 gcatttggcg gtagaagaag gtcaggagca gggtgcggat gtgcgcgccg tccacgtcgg
     2581 catcggtcat gatgatgatg cggtggtaac gcagtttttc ggcattgaat tcttctttgc
     2641 cgatgcccgc gcccaacgcg gtaatcaaag cggctacttc ctgactggcg agcatttttt
     2701 caaagcgcgc tttttcgacg ttcaaaatct tgcccttaag cggcaaaatc gcttggaatt
     2761 tgcggtcgcg cccctgcatg gcggaaccgc ctgcagaatc gccctcgacc agataaagtt
     2821 ctgacaatgc ggggtctttt tcttggcagt cggcgagttt accgggtagc cccaagccgt
     2881 ccattacgcc tttgcggcgg gtgatttcgc gggctttgcg ggcggcttcg cgcgcgcggg
     2941 cggcatcgac gattttgccg gtgatgattt tggcttcgtt cgggttttct tcaaggaagt
     3001 cggtcagcgc ttggttgatg acttcgttga caacggggcc gatttcgctg gaaaccagtt
     3061 tgtctttggt ttgggatgag aatttggggt cgggcagttt gacggacaac acgcaggtca
     3121 aaccttcgcg catatcgtcg cctgctgttt ccactttggc ttttttggcg acttcgttgg
     3181 cttcgatgta gctgttgatg gtgcgtgtca tcacttggcg caacgcggtc aagtgcgtac
     3241 cgccgtcgcg ctgagggatg ttgttggtga agcactgcac gctttcttgg tagctgtcgt
     3301 tccactgcat cgcgcattcg acgctcatgc cgtctttctc gccgaacgca tagaagattt
     3361 tttcgtgcaa cggcgttttt ttgcggttca tgtattgcac gaaaccggcc acgccgccgg
     3421 aaagggcgaa gctttcgtgt ttgccgtcgc gttcgtcggt caattcgatg tccacgccgt
     3481 tgttcaggaa ggaaagttcg cgaatgcgtt tggcgaggat gtcgaagctg tattcgacgt
     3541 tgccgaaagt ttccgtgctg gcgaggaagc gtacggtcgt gcctttcttg tcggaatcgc
     3601 cgacaacttt cagcggctct tcggtttcgc cgcgcacgaa gcggacgaag tgttctttgc
     3661 cgtcgcggta gatggtcagc gtaacccagt cggacagcgc gttgacgacg gacacgccca
     3721 cgccgtgcag gccgccggag attttgtagc tgttgttgtc gaatttaccg cccgcgtgca
     3781 agacggtcat gatgacttcg gcggcggagc gtccttcttt cgggtggatg ccggtgggca
     3841 taccgcgccc attgtcggcg acgctgacgg aatggtcggc gtggatggta acggtgattt
     3901 tgtcgcaatg tccggcgagt gcttcgtcaa tggcgttgtc caatacttcg aacaccatgt
     3961 ggtgcaggcc gctgccgtcc tgcgtgtcgc cgatgtacat accggggcgt ttgcgtaccg
     4021 cttccaagcc ttcgagcacc tgaatgctgt cggcgccgta ttcttcgtgt ttttgttcag
     4081 tcatattttt tgccggattt tgaaaagatt ttgcgatgcc gccaaaacaa gtccgcacct
     4141 tgtggaaaaa gcgggcggga cgacatatat aattgtgtat tatagccgat tttgccgcct
     4201 aattcagcgt tatccgcatc agtgtgccgc cgggaaaaga tgaaacggta cgtttgcctc
     4261 cggcatcagg tcggggattg tcccgtaaag tggcaaaagc gtttttttgc cactaaaatc
     4321 tacaccctat acttttcgga caggggcgcg gaaatggaaa tatggaatat gttggacact
     4381 tggctcggtg ccgtcccgat acgtgcggag gcggtcgaat ccgtggcggt ggtcgcggct
     4441 ttgctgctgg cgcgcgccct tctgttgaat atccacttca aacggcatcc ggatttcggc
     4501 atcgaaagca agcggcggtt tttggttgcc agccgcaata taacgctgct tttggtgctg
     4561 ttttcgctgg catttatctg gtcggcgcaa atccaaacgc tggctttgtc gatgtttgcg
     4621 gtggcggcgg cggtcgtcgt ggcgacgaag gaactgatta tgtgtctgtc gggcagtatt
     4681 ttaaggtctg ccacccagca atactcggtc ggcgactata tcgaaatcaa cggcctgcgc
     4741 gggcgcgtgg tcgacatcaa cctgttgaac acgctgatga tgcaggtcgg tccgaacccc
     4801 ttggtcggac agcttgcggg aaccaccgtt tctttcccca acagcctgtt gttgagccac
     4861 cccgtgcgcc gcgacaatat tttgggcgac tatgtcatcc atacggtcga aatccccgtt
     4921 cccatccatt tggattcgga tgaagccgta tgccgtctga aagccgtact cgagcccttg
     4981 tgcgcgccct acatccccgc catccaacgg catttggaaa acgtgcaggc ggaaaaactg
     5041 tttatcacgc ccgccgccag accgcgcgtt acccgcgtgc cgtacgatga caaggcatac
     5101 cgcatcatcg tccgcttcgc ctcccccgtt tcaaagcggc tggaaatcca acaggcggtt
     5161 atggacgaat ttttgcgcgt acaataccgc ctgttaaatt accccgccgg ctccgaaaca
     5221 ctttaacttt ccccgaccga ccccatttcc ggcttcagac ggcatattgc cgatatgctg
     5281 tctgaagcac aacacgcaaa ggaaacccat cttatgactg acaacgcact gctccatttg
     5341 ggcgaagaac cccgttttga tcaaatcaaa accgaagaca tcaaacccgc cctgcaaacc
     5401 gccatcgccg aagcgcgcga acaaatcgcc gccatcaaag cccaaacgca caccggctgg
     5461 gcaaacactg tcgaacccct gaccggcatc accgaacgcg tcggcaggat ttggggcgtg
     5521 gtgtcgcacc tcaactccgt cgccgacacg cccgaactgc gcgccgtcta taacgaactg
     5581 atgcccgaaa tcaccgtctt cttcaccgaa atcggacaag acatcgagct gtacaaccgc
     5641 ttcaaaacca tcaaaaactc ccccgagttc gacaccctct cccccgcgca aaaaaccaaa
     5701 ctcaaccacg atctgcgcga tttcgtcctc agcggcgcgg aactgccgcc cgaacagcag
     5761 gcagaactgg caaaactgca aaccgaaggc gcgcaacttt ccgccaaatt ctcccaaaac
     5821 gtcctagacg cgaccgacgc gttcggcatt tactttgacg atgctgcacc gcttgccggc
     5881 attcccgaag acgcgctcgc catgtttgcc gctgccgcgc aaagcgaagg caaaacaggc
     5941 tacaaaatcg gtttgcagat tccgcactac ctcgccgtca tccaatacgc cgacaaccgc
     6001 gaactgcgcg aacaaatcta ccgcgcctac gttacccgcg ccagcgaact ttcagacggc
     6061 ggcaaattcg acaacaccgc caacatcgac cgcacgctcg caaacgccct gcaaaccgcc
     6121 aaactgctcg gcttcaaaaa ctacgccgaa ttgtcgctgg caaccaaaat ggcggacacg
     6181 cccgaacaag ttttaaactt cctgcacgac ctcgcccgcc gcgccaaacc ctacgccgaa
     6241 aaagacctcg ccgaagtcaa agccttcgcc cgcgaaagcc tgaacctcgc cgatttgcaa
     6301 ccgtgggact tgggctacgc cagcgaaaaa ctgcgcgaag ccaaatacgc gttcagcgaa
     6361 accgaagtca aaaaatactt ccccgtcggc aaagtattaa acggactgtt cgcccaaatc
     6421 aaaaaactct acggcatcgg atttaccgaa aaaaccgtcc ccgtctggca caaagacgtg
     6481 cgctattttg aattgcaaca aaacggcgaa accataggcg gcgtttatat ggatttgtac
     6541 gcacgcgaag gcaaacgcgg cggcgcgtgg atgaacgact acaaaggtcg ccgccgtttt
     6601 tcagacggca cgctgcaact gcccaccgcc tacctcgtct gcaacttcgc cccgcccgtc
     6661 ggcggcagag aagcgcgttt gagccacgac gaaatcctca tcctcttcca cgaaaccgga
     6721 cacgggctgc accacctgct tacccaagtg gacgaactgg gcgtgtccgg tatcaacggc
     6781 gtagaatggg acgcggtcga actgcccagc cagtttatgg aaaatttcgt ttgggaatac
     6841 aatgtattgg cgcaaatgtc cacccacgaa gaaaccggcg agcccctgcc gaaagaactc
     6901 ttcgacaaaa tgctcgccgc caaaaacttc caacgcggaa tgttcctcgt ccgccagatg
     6961 gagttcgccc tcttcgatat gatgatttac agcgaagacg acgaaggccg tctgaaaaac
     7021 tggcaacagg ttttagacag cgtgcgcaaa gaagtcgccg tcatccaacc gcccgaatac
     7081 aaccgcttcg ccttgagctt cggccacatc ttcgcaggcg gctattccgc aggctattac
     7141 agctacgcgt gggcggaagt attgagcgcg gacgcatacg ccgcctttga agaaagcgac
     7201 gatgtcgccg ccacaggcaa acgcttttgg caggaaatcc tcgccgtcgg cggatcgcgc
     7261 agcgcggcag aatccttcaa agccttccgc ggccgcgaac cgagcataga cgcactcttg
     7321 cgccacagcg gtttcgacaa cgcggcctga cggcaagggt gaagtaaaaa ataggaggat
     7381 tcgatataaa aacatccgca ccgtcattcc cgcgtaggcg ggaatccaga ccggtcggca
     7441 cggaaactta tcggataaaa cggtttcttt agattttacg ttctagattc ccgctttcgc
     7501 gggaatgacg cggaaaagtt gctgtgattt cggataaatt ttcgcaacgt ttaatttccg
     7561 ttttacctga taaatgcccg caatctcaaa tcccgtcatt ccccaaaaac aaaaaaaatc
     7621 aaaaacagaa atc
//
LOCUS       DAXHMX010000097         7585 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHMX010000097 DAXHMX010000000
VERSION     DAXHMX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.097"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(53..361)
                     /locus_tag="ACFM4S_001517"
     CDS             complement(53..361)
                     /locus_tag="ACFM4S_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051166.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="ACFM4S_001518"
     CDS             complement(345..647)
                     /locus_tag="ACFM4S_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6051167.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /gene="pheT"
                     /locus_tag="ACFM4S_001519"
     CDS             complement(721..3084)
                     /gene="pheT"
                     /locus_tag="ACFM4S_001519"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6051168.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAMERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAAHGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3109..3759)
                     /locus_tag="ACFM4S_001520"
     CDS             complement(3109..3759)
                     /locus_tag="ACFM4S_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFC6051169.1"
                     /translation="MITISNEDNMILMSRYPDKYFDLAIVDPPYGILNKTKRGGDYKF
                     NMNEYSQWDIKPDQTYFNELFRVSKNQIIWGGNYFGELWLRSEYNKGFIIWDKNQPET
                     LNNFSMAEMAWSSFDRPSKIFRFSVRKNRNKTHPTQKPVELYQWLLKMYAKQGDKILD
                     THLGSGTLAIACCIAQFDLTACEINSDYYQQSIEKIKNNLPEARISFGHPGYCIIE"
     gene            complement(3756..5225)
                     /locus_tag="ACFM4S_001521"
     CDS             complement(3756..5225)
                     /locus_tag="ACFM4S_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6051170.1"
                     /translation="MTTARWVIDKHLQNFHILCKFAGILKTNSFISVEDKAKLSEKLE
                     KLDLYHRRNTGKSLDATTHKIKELSFYMFGYRDVCGQVTQKFLFSPLGNLFLKHLDNN
                     EYIQKIFLTMLWAIPFPHPYIKTDESIQLYPMRLIFKLLSDERLDCKLFSYEYIYLIS
                     FVKSADQNSYEKLVQDILVLRTCAEVKIKHQLTAENSRSHAYVNAAHEWESYFSKTLT
                     DAGVLQKTDGKIICRLKHGKTETYRKVTSSEFSIPKQLQEFVKKLQSAYSFSEMPLNL
                     NDSDRLKIDVIKEIYSFYPKELLEEIGELKDEAAYELLHLPRLIEQYADNNNGTEAYL
                     FEDVLEMGFNMFYNVEAKKIGGPGNTDLECLYITQKRKFAVEAKSTKNKLSGINSGRL
                     EDHKNKIKAIYTIVVTPRYVPAVLSDIRNCPIVIIRANTFAEFLYNCLINRSSIPEID
                     YRYFDEIIIKNLGKDISSEISNLTMQQFASNTTMEAYST"
     gene            complement(5228..6286)
                     /locus_tag="ACFM4S_001522"
     CDS             complement(5228..6286)
                     /locus_tag="ACFM4S_001522"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6051171.1"
                     /translation="MMIGASLFSSAGIAETYLHNAGIKIIAANELVPERANLYKALYP
                     ESKMIIGDILHEEVFQNLIQSVPNRLDFLIASPPCQGMSVAGKNRNIQEMANDKRNHL
                     IFRVIEFILLKRPTFVLIENVPFFLKIKLPYKGTLQTVEVILQDLFGCEYYIQTHIFD
                     SADYGVAQHRKRAIIRMNKHSTIWGMPEKVTKTISVRDAISFLPSIESGQKSNVKWHF
                     ARTHAPEHIIWLKNTPTGRSAFDNIEHYPKKKNGEKIKSYNTTYRRMEWDAPAPTITI
                     RNDAISSQLNVHPGRSMPDGTYSDARVLTPLELMLLTSLPIDWNIPDDTSELLIRQCI
                     GESIPPLLIKKIVERIGK"
     gene            complement(6469..7461)
                     /gene="pheS"
                     /locus_tag="ACFM4S_001523"
     CDS             complement(6469..7461)
                     /gene="pheS"
                     /locus_tag="ACFM4S_001523"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6051172.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
BASE COUNT         2073 a         1800 c         1492 g         2220 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg attatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cacgtccggg atgcagggcg ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacatctgcc ttaatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccacccc attgttcggg cattgccgcg ccgtaccaca gaccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgtgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc ggcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcagtccg acctgcttgg cttcgggcaa tttaccttgt gcttcaacca tctcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgt tccatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgtcta caccatcatc
     2701 aggcaaacct agttcattgg tcgaacacag cataccgttt gatggcacac cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttca gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatggttaa tttctctata tttttaagtt attcaataat
     3121 acaataacct ggatgcccaa aactgattct agcttcaggt aaattatttt ttattttctc
     3181 aatcgattgt tggtaataat cggaattgat ttcacaagct gtcaaatcaa actgtgcaat
     3241 gcagcatgca atagcaagag ttccacttcc taaatgtgta tctaaaatct tatcaccctg
     3301 ctttgcatac atttttaaca accactgata taattcgact ggtttttgtg ttgggtgagt
     3361 tttattacga tttttccgca cactaaaccg gaaaatttta gatggcctat cgaatgacga
     3421 ccaagccatt tccgccatag aaaaattatt taatgtctct ggttgattct tatcccaaat
     3481 aataaatcct ttattatatt cacttctcaa ccataactcg ccaaaataat tcccacccca
     3541 aataatttga ttttttgaca cgcgaaataa ttcattaaag taagtttggt ctggcttaat
     3601 atcccattgt gagtattcat tcatattgaa tttataatca ccaccacgtt tagttttatt
     3661 caaaatccca taaggaggat ctacaattgc caaatcaaaa tacttgtcag gataccgaga
     3721 cattaagatc atgttatctt catttgaaat agttatcatg tactatacgc ttccattgtg
     3781 gtgttacttg caaactgttg catagtcaaa ttggaaattt ctgaactaat atcttttcca
     3841 agatttttaa taataatttc atcaaaatac cgataatcaa tctctggaat actggagcga
     3901 ttaatcaaac aattatataa aaattcagca aatgtattgg cacgaataat tacaattggg
     3961 caattacgaa tatcggataa tacggcaggg acataacgtg gtgtgacaac aattgtgtaa
     4021 atggccttaa ttttattttt atgatcttcc aatcttcctg aattaatacc tgataactta
     4081 tttttagttg attttgcctc cactgcaaat tttctctttt gcgtaatata taagcactct
     4141 aaatccgtat tacctggtcc accaattttt ttagcttcta cgttataaaa catattgaac
     4201 cccatttcta gaacatcttc aaatagatat gcctctgttc cattattatt atctgcatat
     4261 tgttcaatca acctaggtaa gtgcaataat tcatatgctg cttcatcctt aagctcacca
     4321 atttcctcca ataactcttt tggatagaag ctataaattt ccttaatgac atcaattttc
     4381 aaacgatcac tatcgttcag atttaatggc atttctgaaa acgaataagc actttgcaat
     4441 tttttcacaa attcctgaag ttgcttagga atcgaaaact cacttgatgt tactttacga
     4501 tatgtttcgg tcttaccatg ctttagacgg caaataattt taccatctgt tttttgcaaa
     4561 acacctgcat cagtcaatgt ttttgaaaaa taagattccc actcatgtgc tgcatttaca
     4621 taagcatgac tacgactatt ttccgcagtt aattgatgtt taatttttac ttcagcacat
     4681 gttcgtaaca ccaaaatgtc ttgtactaat ttttcatagc tattctgatc agcagatttc
     4741 acaaatgaaa ttaaatagat atattcataa gaaaatagtt tacaatccaa tctttcatca
     4801 gataacaact taaatattag tctcatggga tataattgaa tactttcatc agtcttaatg
     4861 tacggatgag gaaatggtat cgcccacaac atagtaagaa aaattttttg aatatattca
     4921 ttattatcca agtgtttcaa aaataaatta cccaatggac tgaacaggaa tttctgtgta
     4981 acttgcccac acacatcacg ataaccaaac atatagaatg ataattcttt tattttatga
     5041 gtagtagcat ccaatgattt acctgtattt cgtctatggt ataaatctag tttttccaat
     5101 ttttcagata acttagcttt atcctctaca gatataaaag aatttgtttt caaaatacct
     5161 gcaaatttac ataaaatatg aaaattctgt aaatgtttat ctattaccca gcgcgcagtt
     5221 gtcatatcta ttttcctatt ctctctacaa tttttttaat taacaatgga ggaatagatt
     5281 caccaataca ttgccgaatt aataattctg atgtatcgtc gggaatattc caatcaatag
     5341 gtaatgatgt taaaagcatt aactctaatg gtgttaaaac tcttgcatct gaatatgttc
     5401 catcaggcat agaccgtcca ggatgaacat ttaattgtga actgatagcg tcattacgaa
     5461 tagtaatagt tggggcagga gcatcccact ccatacggcg ataagttgta ttataacttt
     5521 taattttttc accatttttt ttctttggat aatgttctat attatcaaaa gcagatcgtc
     5581 ctgttggcgt attttttagc catataatgt gctccggagc atgtgtacgt gcaaaatgcc
     5641 atttcacatt agacttttgt ccagactcaa tactaggcaa aaaactaata gcatcacgaa
     5701 cagaaatggt ttttgtaact ttttccggca ttccccaaat agttgaatgt ttattcatac
     5761 gaataatagc tcgtttacga tgttgtgcaa caccataatc ggcagaatca aaaatatgag
     5821 tttgaatata atattcgcaa ccaaataaat cttgcaaaat tacttctact gtttgtaatg
     5881 tccccttata aggtaactta atttttaaaa aaaatggaac attttcaatc aaaacaaaag
     5941 ttgggcgttt taataaaata aattcgatta ctctgaatat taaatgatta cgtttatcat
     6001 tagccatctc ttgaatgtta cgatttttcc ctgcaacact catgccttga caaggaggag
     6061 aagcaattaa aaaatctaat cgattcggca cgctctgtat taaattttga aacacttcct
     6121 catgaagtat atcacctata atcattttac tttcgggata tagagcttta tataaattag
     6181 cacgttctgg caccaattca tttgcagcta taatcttaat acccgcatta tgcaagtagg
     6241 tttctgcaat ccctgcactc gaaaacaaag aagcccctat catcatagaa ccaaacccaa
     6301 attaactata taatctctac aaagcaataa attatgcctc tttaaacaaa attaggaatg
     6361 taatttcaca acaaaatatc aaatgtttta tatcttacat ttgaattttc ctaagcagcc
     6421 tgaaaaccaa gagtaggctg cttttccata ttcaggcagc ctacacaatc attttacaaa
     6481 ctgcttcaaa aaattcaaat cgttatcaaa aaacaagcgc aaatcgttca cgttgtaacg
     6541 cagcatcgcg aaacggtcga gaccgatacc gaaagcgaaa ccggtgtatt tttcagaatc
     6601 gatattcaca tttttcaaca cgttgggatg caccatgccg cagccgccga cttccagcca
     6661 tttgccgttt tcgcccataa tgtcgatttc ggcagacggt tcggtgaacg ggaagaaaga
     6721 cgggcggaaa cgtacttgca aatcatcgcg ttcaaagaag cgacggataa aatccgtgaa
     6781 cactgctttc aaatcggcaa aagttacgcc ctcttctacc cacaaacctt ccgcctgatg
     6841 gaacataggc gagtgcgtgg catcgctgtc cacacggtaa acgcggccgg gggcgataat
     6901 gcggatgggc ggctcttttt tatcgagcat atagcggatt tgaatcgggg aagtgtgcgt
     6961 acgcaaaaca tcaccgtttt caacgtaaaa cgtatcctgc atcgcacggg caggatggtt
     7021 tgcagggatg ttcagggctt ggaaattgtg aaaatcgtct tcgatttcag gcccgtccgc
     7081 cacttcgaaa cccattccgt gaaagagttc gaccacacgt tgcaaggtca gggttacggg
     7141 atgcaggctg ccgccttcct gagcgcgtcc gggcagggta atgtcgaggg cttcggcggc
     7201 aagtcgggct tgcagcttgg cttcgttgag ggcatcgcgt ttggcattaa aagccgtctg
     7261 aaaccggttt ttgcattcat tgatatgcgc acctatggtt ttgcgctctt caggcgacat
     7321 ttgccccaaa gttttcagaa gtccggtcaa ctcgccggtt ttgccaagat aacgggcttt
     7381 gatttgttct agagcgttga agtcttgcgc agcttctact gcggcaatgc cttctgcaac
     7441 gatgcggttt acattttcca taatatcaag tctgtcaatt aatgatatgc cacctgaaac
     7501 aacgaggccg cctgtatagc ctgacatatc agactcttca gacgacctta ttttcttcac
     7561 ggtcaatcca ttggataaat tttct
//
LOCUS       DAXHMX010000098         7549 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHMX010000098 DAXHMX010000000
VERSION     DAXHMX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.098"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..419
                     /locus_tag="ACFM4S_001524"
     CDS             <1..419
                     /locus_tag="ACFM4S_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6051173.1"
                     /translation="EAEWAGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKA
                     AAAGHGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHG
                     NLGWLLFAAVAGHVAMVVTHRVQGRDVLCRMTGRVR"
     gene            complement(444..1319)
                     /gene="rsmI"
                     /locus_tag="ACFM4S_001525"
     CDS             complement(444..1319)
                     /gene="rsmI"
                     /locus_tag="ACFM4S_001525"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6051174.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLILYPAQDEKHEGLSESAQNVMKILAAELPTKQAAELAAKITGE
                     GKKALYDLAVSWKNK"
     gene            1373..1720
                     /locus_tag="ACFM4S_001526"
     CDS             1373..1720
                     /locus_tag="ACFM4S_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6051175.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1725..2318
                     /locus_tag="ACFM4S_001527"
     CDS             1725..2318
                     /locus_tag="ACFM4S_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6051176.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDILVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2382..2990
                     /locus_tag="ACFM4S_001528"
     CDS             2382..2990
                     /locus_tag="ACFM4S_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6051177.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            2998..3648
                     /locus_tag="ACFM4S_001529"
     CDS             2998..3648
                     /locus_tag="ACFM4S_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051178.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3688..4467
                     /gene="map"
                     /locus_tag="ACFM4S_001530"
     CDS             3688..4467
                     /gene="map"
                     /locus_tag="ACFM4S_001530"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6051179.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4622..4921
                     /locus_tag="ACFM4S_001531"
     CDS             4622..4921
                     /locus_tag="ACFM4S_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6051180.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5159..5533
                     /locus_tag="ACFM4S_001532"
     CDS             5159..5533
                     /locus_tag="ACFM4S_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6051181.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5720..7186)
                     /locus_tag="ACFM4S_001533"
     CDS             complement(5720..7186)
                     /locus_tag="ACFM4S_001533"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6051182.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1954 a         2287 c         1840 g         1468 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgctgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaagtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgc cggacacgtc gccatggtcg
      361 tcacccaccg tgttcagggt agagatgttc tgtgccgcat gacggggcgt gtccgctgat
      421 tccgttcgca ctatggtgcc gcactatttg tttttccacg acactgccaa atcgtacaaa
      481 gcctttttgc cctcacctgt aatcttggcg gcaagctcgg cagcctgttt ggtcggcagt
      541 tcggcagcaa ggattttcat cacattttgc gcggactcgg acaagccttc gtgtttttca
      601 tcctgcgccg gatacagtat caacaccatc tcgccgcgcg attggttgcc gtcggcagcc
      661 aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt tttcgtgatt
      721 tcgcgcgcca gcatcagacg gcgttcgggg aacagttccg ccatatcggc aagcgtcgcc
      781 ccgatgcggt gcggcgtttc aaacataacg ataggaaacg ccgcccgcac ccatttggca
      841 aacaatttcc tacgttcgcc cgatttcggc ggtacaaaac cgttgaaata aaaatcggat
      901 cccgccacac cagccacact caaagccgcc atcaccgcgc ttgcgccgac aacagggaca
      961 actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc gcacacggcc
     1021 ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata gccgacaatc
     1081 ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga cgagtttgcc ctgaatgccg
     1141 tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat gtccgccttt
     1201 tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg cgtggcaacc
     1261 acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt ctgaaacata
     1321 aaaaatgccg tctgaaaaac aaacattata aattataaag gttaaaccga ttatgcgcct
     1381 aaaccacaaa cagggcgagg caggggaaga tgccgcgctt gccttcctcc aatcccaagg
     1441 ctgcacactg cttgcccgca actggcactg cgcctacggc gaaatcgacc tgattgtcaa
     1501 aaacggcggc atgattctgt ttgttgaagt aaaataccgc aaaaatcggc aattcggcgg
     1561 tgccgcatac agcatttccc catccaaatt attgaaactg caacgaagtg tagagtatta
     1621 tctgcaacag aacaggttga caaacgtacc gtgccgcctc gacgcggtac tcatcgaagg
     1681 cagccgcccg cccgagtgga tacagaatat tacaggttga cgatatgacg acattacaag
     1741 aacgcgttgc cgcccatttt gccgaaagca tccgtgccaa gcaggaagcc ggaaaagtat
     1801 tggtcgagcc gaccgtacag gctgccgagc tgatgctgca atgcctgatg aacgacggca
     1861 agattctggc gtgcggcaac ggcggttcgg ctgccgacgc gcagcacttc gccgccgaaa
     1921 tgaccggacg ctttgaaaaa gagcgcatgg aactcgccgc cgtcgccctg accaccgaca
     1981 catccgcgct gaccgccatc ggtaacgact acggcttcaa ccacgtcttc agcaaacagg
     2041 tacgcgccct cggacgcgcc ggcgacatcc tcgtcggcat ttccacttcc ggcaattccg
     2101 ccaacgtcat cgaagccatt aaagcggcac acgaacgcga tatgcacgtc atcgccctga
     2161 ccggccgcga cggcggcaaa atcgccgcca tactcaaaga caccgacgtt ctgctcaacg
     2221 tcccccatcc gcgcaccgcc cgcattcagg aaaaccacat cctgctgata cacgccatat
     2281 gcgactgtat cgactccgta ctgctggaag gaatgtaacc cttttcagac ggcatggcgc
     2341 aaagcaatgc cgtctgaaac gcccaagaaa ggaagccccc gatgaaaccc aaaccgcaca
     2401 ccgtccgcac cctgattgcc gccattttca gccttgccct tagcggctgc gtcagcgcag
     2461 taatcggaag cgccgccgtc ggcgcgaaat ccgccgtcga ccgccgaacc accggcgcgc
     2521 aaaccgacga caacgttatg gcgttgcgta tcgaaaccac cgcccgctcc tatctgcgcc
     2581 aaaacaacca aaccaaaggc tacacgcccc aaatctccgt tgtcggctac aaccgccacc
     2641 tgctgctgct cggacaagtc gccaccgaag gcgagaaaca gttcgtcggt cagattgcac
     2701 gttccgaaca ggccgccgaa ggcgtataca actacattac cgtcgcctcc ctgccgcgca
     2761 ctgccggcga catcgccggc gacacttgga acacatccaa agtccgcgcc acgctgttgg
     2821 gcatcagccc cgccacacag gcgcgcgtca aaatcgttac ctacggcaac gtaacctacg
     2881 ttatgggcat cctcaccccc gaagaacagg cgcagattac ccaaaaagtc agcaccaccg
     2941 tcggcgtaca aaaagtcatc accctctacc aaaactacgt ccaacgctga ctcggcaatg
     3001 ccgtctgaac cgccttcaga cggcattgcc cgacacccca aaagcacaat caaaatggca
     3061 aaaaaaccga acaaaccctt caggctgacc cccaaactcc tgatacgcgt cgtattgctc
     3121 atctgtatca ccgccatcgg cgcattggca ataggcatcg tcagcacatt caacccgaac
     3181 ggcggcaaaa ccctccaaac cgaaccgcaa cacaccgaca gcccccgcga aaccgaattc
     3241 tggctgccaa acggcgtagt cggacaagat gccgcccaac ccgaacacca ccacgccgcc
     3301 tcatccgaac ccgcacagcc ggacggcaca gacgaaagcg gcagcggact gccgtcccct
     3361 gccgcaccca agaaaaaccg ggtcaaaccg caacctgccg acacagctca aaccgacagg
     3421 cagccggacg acgccggaac acaagctgaa aacacactca aagaaacccc cgtactgccc
     3481 acaaacgtcc cccgtcccga accccgaaaa gaaacacccg aaaaacaggc gcagcccaaa
     3541 gaaacaccca aagaaaaaga aacgcccaaa gaaaaccata ccaaaccgga caccccgaaa
     3601 aacacgccgc ccaaacccca taaagaaatt ctcgacaacc tcttctgacc cggcacggca
     3661 ggcacacccg caatccaagg aagcattatg aacggcatca tcatcaaaac ccccgaagaa
     3721 atcgaaaaaa tgcgcgagct gggcaaactc gtcgccgaag ccctcgacta catcggacaa
     3781 ttcgtcaaac ccggcgtaac caccgacgaa atcgacaaac tcgtttacga ctaccacgtc
     3841 aacgtccaag gcggctatcc cgcccccctg cactacggca acccgcccta ccccaaatcc
     3901 tgctgcacct ccgtcaacca cgtcatctgc cacggcattc ccgacgacaa gccgctcaaa
     3961 gaaggcgaca ttatcaacat cgacctcacc atcaaaaaag acggcttcca cggcgactcc
     4021 agccgtatgt ttaccgtcgg caaagtctcc cccatcgccc aacggctgat cgacgtaacc
     4081 cacgcctcca tgatggcggg catagaagcc gtcaaacccg gcgcgacact gggcgacgta
     4141 ggctacgcct gccaacaggt tgccgaaaac gccggctatt ccgtcgtaca ggaattctgc
     4201 ggacacggca tcgggcgcgg tttccacgaa gccccgcaag tgttgcacta cggaaaaaaa
     4261 gggcagggcc ccgttctaaa accgggtatg atttttaccg tcgaaccgat gatcaaccaa
     4321 ggcaaacgcc acctgcgtat cctcaacgac ggctggacgg tggttaccaa agaccgctcc
     4381 ctctccgccc aatgggaaca cgaagtcttg gtgaccgaaa ccggctacga aatcctcacc
     4441 gtcagccccg cctccggcaa accctgaagc cggacgtatc cgccccataa aaacaaacaa
     4501 tgccgtctga aagaaacggc agatatgata tataatataa aaacaggctt gacccggcac
     4561 attacgaaaa caaagcaaat cggaatttgc cccgcaacca gacaaactta aaggaagttt
     4621 tatgaaaata tttgaaaata tagaagatgt taaagccatc cgtaaaaaga ccgggctgaa
     4681 ccagatagac ttctggggca aggtcggcgt tacccagtct ggaggctcgc gctacgaaac
     4741 cggccgcaaa atgcccaaac ccgtacgcga actgctccgc ctcgtccata tcgaatgcat
     4801 cgatttggcg aaagtcaaca aaaaagatat ggaaatcgcc gccctgttga aaaaacacca
     4861 tcccgacctg tatgccgaat tgtccaaaca gaccaagtcc gaaagaaaaa aacaaagtta
     4921 aaccgcaacc tccggatgcc cgaaagtttt tcatttccga aaaacgcaaa caatgccgtc
     4981 tgaaacaccg gacaggtcgc cgtatcccgc ctgccgcccc tgcctcaaac cgccgaaccg
     5041 cccgaacccg cctttttaca aactttatcc aatttcctgt ttatttcagg atacgccgac
     5101 attagaatgt caaacagctc gaaacgggca aactccacat ccatccaaag gaataaaaat
     5161 gaaacttctg accaccgcaa tcctgtcttc cgcaatcgcg ctcagcagta tggctgctgc
     5221 tgccggcacg aacaacccca ccgttgccaa aaaaaccgtc agctacgtct gccagcaagg
     5281 taaaaaagtc aaagtaacct acggcttcaa caaacagggc ctgaccacat acgcttccgc
     5341 cgtcatcaac ggcaaacgcg tgcaaatgcc tgtcaatttg gacaaatccg acaatgtgga
     5401 aacattctac ggcaaagaag gcggttatgt tttgggtacc ggcgtgatgg atggtaaatc
     5461 ctaccgcaaa cagcccatta tgattaccgc acctgacaac caaatcgtct tcaaagactg
     5521 ttccccacgt taatcaggca acaaaaaaca gcgttttcag aaatgaaaac gctgtttttt
     5581 gactgttcca ttattcacaa aagggaaaaa acgattacct gccccgtgta ccaaaacctg
     5641 ccctgccgga tgaagggcat aactggcagg gacggcgtca acaccatatg ggggtacggc
     5701 ttttcttgaa agattcggct taaatatcca atactttcgc ggtataggcg ataatttcat
     5761 ccgccctttc agggttttcg ttcaacttga tgccgtaacc cggtaccagc tctttcagac
     5821 ggtcttccca agacggggcg cgctcgggga agcattggtg catcagccgg atcatcagcg
     5881 gcacagcggt cgatgcgccc ggcgacgcgc ccagcaatgc ggcgagtgag ccgtcggcgt
     5941 gggcgacaat ctccgtacca aactggagca cgccgccttt ttcggagtct tttttaatga
     6001 tttggacgcg ttgccctgcg gtgatgagtt cccagtcgtc ggggtttgcc tcggggtagt
     6061 attccagcag ggaggcgaag cgttcttctt tggttttacg caattcgccc agcaggtatt
     6121 tggtcagcgg catattcgcc cagccggcgc acagcatagg atagaggttg tccatatgga
     6181 tggacagcgg caaatccata agcgagcctt gcttgaggaa gttggaacgg aagcctgcgt
     6241 aagggccgaa cataaggtgg cgtttgccgt ccacgttgcg tgtgtcgagg tgcgggacgg
     6301 acatcggcgg cgcgccgacg gaagcctgcc cgtacacttt ggcgttgtgt tgttcggcgg
     6361 tttcggggtt gctgttgcgg aagaacaggc cggacacggg gaagccgccg tagcctttgc
     6421 cttcggggat gccggatttt tgcagcaggg tcagcgcgcc gccgcccgcg ccgaggaaga
     6481 ggaagcgggt acggagggtg agctgcccgt cggggttgcg ggtatcggcg gttttgagca
     6541 cccacgcgcc gtcggattcg cgtttgatgt cttcgacgtg gcggttgaac tcggttttta
     6601 cgcccttgcc ctgcaaatat ttcaccattt ggcgcgtcag ccgtccgaaa tcgacatccg
     6661 taccttcggc ggagtagttg gcggcgacgg gttggttttc gtcccggccg cgcatcatca
     6721 gcggagccca atcggaaatt ttgttccgat cggtggaaaa ttccatattt tcaaaaagtt
     6781 tttgggtttt aaacgcgtca taacgttttt gaagataaga acaatggtct tcattcatca
     6841 ccaaagacat atgcggcacg gcattgatga aggaattgtc ttccaacttg ccttccgcga
     6901 ccagcgtcgc ccaaaactgg cggctgacat gaaactgttc ggcaatattg agggcgcgcg
     6961 ccggatcgat aatcccattt gcacccaacg gcgcatagtt caattcgcac agcgcggaat
     7021 gccccgtgcc ggcgttgttc cacgcgtttg acgattccaa cgccacatct tccaagcgtt
     7081 caatcagggt gatttcccaa gacggttcga gttctttgag caaaacgccc aaagtcgcgc
     7141 tcataatgcc gccgcccacc aagacaacgt ctgtcgcttc agccatggtt tactcctaaa
     7201 aaacaggcat cttctgccct tatggttatt tgccgtacta caaacgcctg aatcgcaaaa
     7261 gcagggaaaa ccgacaatgg tgtgtgtccg agtatgctgt ttcggggttg gaatgcgttg
     7321 caagtatggc ttccgacacc gcttcagggg cttgtaatat gttatcgtga atgtagtgga
     7381 ttttactggg aaatgcaaag tttttctgtc gcccgccaag tcgggaaact gcgaaatgaa
     7441 aaataaaaat agttatttat ctatatatat caaattttta atagataaaa aatcaaaatt
     7501 gtttatatat taatttttaa aagattgtca gcatattgcg ttaagtttt
//
LOCUS       DAXHMX010000099         7518 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHMX010000099 DAXHMX010000000
VERSION     DAXHMX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.099"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..246)
                     /locus_tag="ACFM4S_001534"
     CDS             complement(<1..246)
                     /locus_tag="ACFM4S_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6051183.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGKVEADESYFAERQNHINGIGN"
     gene            complement(551..1810)
                     /gene="rho"
                     /locus_tag="ACFM4S_001535"
     CDS             complement(551..1810)
                     /gene="rho"
                     /locus_tag="ACFM4S_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6051184.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(2030..4414)
                     /gene="ppsA"
                     /locus_tag="ACFM4S_001536"
     CDS             complement(2030..4414)
                     /gene="ppsA"
                     /locus_tag="ACFM4S_001536"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6051185.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4825..5646
                     /locus_tag="ACFM4S_001537"
     CDS             4825..5646
                     /locus_tag="ACFM4S_001537"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6051186.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5886..6548)
                     /locus_tag="ACFM4S_001538"
     CDS             complement(5886..6548)
                     /locus_tag="ACFM4S_001538"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6051187.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6561..7388)
                     /locus_tag="ACFM4S_001539"
     CDS             complement(6561..7388)
                     /locus_tag="ACFM4S_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6051188.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLITRYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT         1577 a         1802 c         2185 g         1954 t
ORIGIN      
        1 gttcccaatt ccattgatat ggttttgtcg ttcagcaaaa taactttcat ctgcttctac
       61 tttgccgtca aacatttcca aatgcggact gttttgataa ataagtaatc gtaaacgatg
      121 aaaataatag gctgcggtat ttttattaac gcctactaac tctgctgctg ttcttgcagt
      181 tacacctgcg acaaacagtt caatgagttt attttgttta tactggctta gacgactttt
      241 tctcataggg gcaactctaa cttaatttga atttccctag ttatctagga cagccccaaa
      301 tttatacaaa aatgagcgcg gttcggcgca accttgaatc aagttcccgc atcggttttc
      361 attgccggta cggatgcgtt caagccggtt ttgcaaaggc cgcgctttcg gcaagcggac
      421 acggacactg ccggcggttg cgccgttaac ggggggaggg aggagctgcg ccggccgtgt
      481 gaatgaaagt gccgtctgaa acccgatttt caggtttcag acggcatttc gcctaatgtc
      541 gatgatgtgt ttattttccg cgcatcagtt caaagaaatc atcattgttt ttagaggctt
      601 tgatttttcc gattaaaaat tcggctgcct cgatttcgtc catcgggtgc aggaacttgc
      661 gcagcagcca catacgttgc agctggtcgt tcggcacaag cagctcctcg cggcgcgtgc
      721 cggatttgtt gatgttgatg gcggggaaga gacgtttttc cgccatacgg cggtcaaggt
      781 gcaattccat attgccggtg cctttgaatt cttcgtaaat cacatcgtcc atacggctgc
      841 cggtttcaac caatgctgtg gcgatgatgg tcagcgaacc gccttcttcc acgttgcgcg
      901 ccgcgccgaa gaaacgtttg gggcggtgca gcgcgttggc atcgacaccg ccggtcagga
      961 ttttgcccga ggtaggcacg acggtattgt aggcgcgggc aaggcgggta atcgaatcaa
     1021 gcaggatgac cacatctttt ttgtgttcta ccatacgctt ggctttttca agcaccattt
     1081 cggcaacttg gacgtggcgt tgagccggct cgtcaaaggt ggaggagact acttcgcctc
     1141 ggacggaacg gctcatttcg gttacttctt cgggacgctc gtcaatcaag aggacgatga
     1201 gttcgacttc gggatagttt gcggtaacgg cgtgggcaat gttttgcagc atcacggttt
     1261 taccgctttt gggcggggca accaagaggg cgcgctgacc tttgccgatg ggggaaatca
     1321 ggtcgatagc acgtccggtc aggttttctt cggactttaa gtcgcgttcc agcttcaact
     1381 gttcggtcgg aaacagcggg gtcaggtttt caaacaggat tttatggcgg catacttccg
     1441 ggtggtcgcc gttgatggta tcaagcctga ccagggcaaa atagcgttcg ttgtcttttg
     1501 ggacgcgcac gcttccttcg atggtgtcgc ccgtatgcag gttgaagcgg cggatttggg
     1561 tgggtgagac atagatgtcg tcgggaccgg caagatagga cgtgtccgcg ctgcggagga
     1621 agccgaagcc gtcgggcagg atttcaagcg tgccggagca ggtgaaaccc tcgccttttt
     1681 tcatcatctg gcggacgatg gcaaatacga ggtcttgttt gcggaatcgg ttggcgtttt
     1741 cgatgccgtg ttcttccgcc aattctaaga gtttggaaat gtgcagggtt tgtaattcgg
     1801 agacgtgcat aataatgatg tattttgaag aggaaaaaga caggcagatg ccgtctgaaa
     1861 gaagaagctg accgttgccg gttgctcggg gaagggggaa ttgtaggcag tcggcgcgtg
     1921 ggtgtcaaat attatcgcgg acggggcatc ggcagaaaat gccgtctgag cggagctgct
     1981 tggaaaaaaa tacccccgcg cttttcaggc tcgggggtat gggcattgat tatttgttca
     2041 attcattcgc caaatacagc caagtttcga tgacggtatc cgggttcagg gaaacgcttt
     2101 cgataccttc ctcaaccagc catttggcga agtccggatg gtcggacggg ccttgaccgc
     2161 agatgccgac atatttgttc tgcttgcggc aggcggagat ggcaaggtgc agcatcactt
     2221 tgacggcagg gttgcgttcg tcaaacgatt cggataccaa gccgctgtcg cggtcgagac
     2281 cgagggtcag ttgggtcatg tcgttcgagc cgatggagaa gccgtcgaag tattgcagga
     2341 attgttccgc caataccgcg ttgctcggca gctcgcacat cataatcagg cgcaggccgt
     2401 ttttgccgcg ttccaagccg ttttctttca gggctttgac aacggcttcg gcttcgccca
     2461 aagtgcggac gaacggaatc atgatttcaa cgttggtcag acccatttca tcgcggacgc
     2521 gtttcaaggc tttgcattcc aaggcgaaac agtctttgaa gttgtcggcg acataacgcg
     2581 ccgcgccccg gaagcccaac atcgggtttt cttcatgcgg ttcgtatacg ttgccgccga
     2641 ccaggttggc gtattcgttg gatttgaagt cggacatacg gacgatggtt ttacgcggat
     2701 aaaccgatgc ggccaatgtc gccacgcctt cggcgatttt atcgacgtag aagtcgacag
     2761 gggacgcgta accggcgata cggcgggtaa tttccgcttt taattcgtcg tcttgtttgt
     2821 caaattccaa caaggctttc gggtggatac cgatttggcg gttgatgata aattccatac
     2881 gcgccaagcc gatgccttcg ctgggcaggt tggcgaagct gaatgcgagt tcggggttgc
     2941 cgacgttcat catgactttg acgggtgctt tgggcatatt gtccaaggcg acatcggtga
     3001 tctgtacgtc caacaggccg gcatagatga agccggtatc gccttcggcg caggatacgg
     3061 tcacttcctg accgttttcc aagagttcgg tcgcattgcc gcagccgacg acggcaggaa
     3121 tacccagttc gcgcgcgatg atggcggcgt ggcaggtacg gccgccgcgg ttggtcacga
     3181 tggcggaagc acgtttcatc accggttccc aatccgggtc ggtcatatcg gtaacgagta
     3241 cgtcgccggc ttcgacggaa tccatctcgg aagcatcttt aatcaggcgt accttgccct
     3301 gaccgacttt ctgaccgatg gcacggcctt cgcataatac ggttttatcg ccgttgatgg
     3361 cgaagcggcg caggttgcgg ctgccttctt cctgggattt gacggtttcg gggcgggctt
     3421 gcaggatgta gagtttgccg tccaagccgt cgcgtcccca ctcgatgtcc atcgggcggc
     3481 cgtagtgttt ttcgatggtc agtgcgtaat gcgccaactc ggtgatttct tcgtcggtaa
     3541 tggagaagcg gttgcggtct tcttcgggga catcgacgtt ggttaccgat ttgccggctt
     3601 cggctttgtc ggtaaaaatc attttgatgt gtttcgaacc catggtttta cgcaggatgg
     3661 cgggcttgcc cgctttgagc gtgggtttga acacataaaa ttcgtccggg ttgaccgcac
     3721 cttgtacgac gttttcgccc agaccgtaag aggaggtaac aaagacgact tgatcgtagc
     3781 cggattcggt gtcgagggtg aacatcacac ctgatgcgcc gctgtcggaa cgcaccatgc
     3841 gttgaacgcc ggcggaaagg gcgacgatat cgtgttcgaa gcctttgtgg acacggtagg
     3901 aaatggcgcg gtcgttgtac agggaggcga atacatggtg catcgcttct ttaacgtttt
     3961 ccaatccgtt gatgttcaag aaggtttcct gttggccggc aaatgatgcg tcgggcaggt
     4021 cttcggcggt tgcggaagag cgtacggcaa cggaaatgtc cgcgccgccg gcatcggcaa
     4081 ccattttgtt ccatgccgct tcgatttcgg catcgagctg ttcggggaag ggtgtgtcca
     4141 aaatccattg gcggatttct ttgccgacgc gtgccagttc ggcaacgtct tcgacatcca
     4201 attttgccag tgcggcggaa atgcgttcgc tcagaccgtt gtgtgcgagg aatgcgcggt
     4261 aggcttcggc tgtggtggca aagccgccgg ggacgcgaac gcctttttcg gtcagctgac
     4321 tgatcatttc gcccagcgag gcgtttttac cgcccacgcg ttcaacatct gtcatacgca
     4381 ggttttcaaa ccaaattacg tagttgtcgg ccatttgtgt gtccaatcca aaatatgtta
     4441 aaaaagaaac aaatccgcgt gcttatttta agcgattcgt tcctctgctg tcatgtgttt
     4501 tatccgtttt aaaatcatga tgccgtctga aaaattgcgg tttcggcgtg tgtagcggtt
     4561 tgaaacttgc agccggtata cttctttttt tgggtatttt ctttgtaaaa caggtggttt
     4621 gaataggtta atgttttttc tgtttgattt ttttgtttat tttttaaaat tttctgccaa
     4681 aaaatacttt atataaataa ttttatttca aaattatatt gtgtctgttt gggtgtaatc
     4741 cgaggtaggc gtgctgcggg tgctttcctt gtgtctgccg ctgctgttat gatgatattt
     4801 ttattctgta tttaaggatg gaagatgagc agtccgcgcc atgtgtttta catttccgac
     4861 cgtaccggtc tgactgccga gaatatcggc gaggctttgc tgaaccagtt tggcaatctg
     4921 tcgttcaaac gccatacgca tccgtttgtc gatacgccgg aaaaggcgcg cgcggtggtg
     4981 gagaaggtca atcggagccg gcaggaaaac ggtcagcgtc cgattgcgtt tgtcagtgtg
     5041 gttgatgacg aaatccgtcg gattatcaaa ggggcggatg cttttcagat taatttcttt
     5101 gagacttttt tgggactgtt ggagaaagaa ctcaataccg aagccacggc atccgagcag
     5161 gggcatcaca gtatcggtaa tacgaagcgt tacgatgcgc gtatggaggc ggtaaatttc
     5221 tcattgaacc acgatgacgg ggtcagcgat aagaaccttc aggaagcgga tgtaatcttg
     5281 atgggtgtat cgcgttcggg caaaacgccg acctgccttt acctcgccct gcaatacggc
     5341 atccgtgcgg caaactatcc gctgattcct gacgatttgg aatcggccga tctgccgcgt
     5401 atggtcaagc cttataggga taagctgttc gggttgacca tccagccgga acgtttgcag
     5461 gccatccgcc aagagcgccg cccgaattca acttatgcca aaatcgatac atgccgcagc
     5521 gaggtggcag acgcgcagag tatgttcaga cggcatggga ttccgtttgc gaatacgacg
     5581 gataaatcag tggaagagct tgccgtacac atccttcagg cgtgcaagct caaacgcagg
     5641 ttttgacggg ttttgattcg gtttgaaggt ggaaatgccg tctgaaatca ggtttcagac
     5701 ggcagtttta tagtggatta acaaaaatca tgacaaggcg acgaagccgc agacagtaca
     5761 aatagtacgg agccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc
     5821 taaggcgagg caacgctgta ctggtttaga tttaatccac tatatgtttg tggggcggat
     5881 atttttcagg gctgtatttt gtccagacat tcgagcagat cgagcggcgt tcggatgtgg
     5941 aaatccgcct gccatgagtc ggtatcgtct tcatctgaga tgtagcccca ttcggcgagg
     6001 acggttttca tgccggcgtt gcggcctgct tggatgtcgc gttcggcatc accgacgtag
     6061 agtgtgtgtt gcgggtcggc gtggatttgt ccgcacgcat acagcatggg tttgatgctg
     6121 ggcttgggtt cgccgcaggt gtcgccgctg acgacgacgg cgggtgggat gatgaagccg
     6181 agtttgggga cgagtttgtc ggtgaagcgc atgggtttgt tggtgatgat gccccatttg
     6241 atgccgcgtt ggttgagttc ggcaatgagt tcgttcacgc cgtcaaagag ctcggtgtct
     6301 tgggcgtagc ggcggtcgta ttcctccaga tattcggtgc gccatcgggc atagtcgggg
     6361 tggtcggggg tgatgcctgc gccgagtttg atcagtcctg ccgcgccgtg gctggcttgg
     6421 gtgcggatct cgtccatgct ttttgcaggt agtccgtggc gagcgagcag ggtgttgagt
     6481 gcgcctccga ggtcgagggc ggtgtcggca agtgtgccgt cgaggtcgaa gagtacggcg
     6541 tgtatcatgt gtgttccttt tcagacggta tttttgttct gtttcaaacc ctgctcgaaa
     6601 tcttccagca tatccaattc aaagcggctg aagcccgctt tttcgcgcgc ttcgatgttc
     6661 acatagccgc ggaagataaa catatcgtaa cgggtaatca gactgcggaa cagggcgaca
     6721 ggttccaaac cgcgttcgcg gcagaggtgt tgataccaat ggttgccgat ggcgacatgg
     6781 cccacttcgt cgcggtaaat gatgtccaat acgccgcagg tttccgaatc gccgcgctgc
     6841 gccaccttcg cgcgtatccc cggcgtaacg tccagcccgc gcgcttccaa cacgcgcggt
     6901 accaacgcca tacgcaacag cgggtcgtag gcggttttgt acgccatatc ccacaaatgg
     6961 ttgtgcgcct cgaaactgcc gtaatcgaag ccgaaagcgc gcaatctgtc gcgcatcagg
     7021 cggaaatggt acacctcttc cttcgccact ttcacccagt cgcggacaaa ctgaaacggc
     7081 agcgtgcgga aacggtatgc cgcgtccaaa gctagattga cggcgttgaa ttcgatatgc
     7141 gcaatcgcgt gcagcatcgc cgcatagcct tctgccgtgt tcattttgcg cggggtcagt
     7201 tgcgacggcg cgaccaaaac aggcttgtcc ggccgtcccg ctcgggggaa gtccgccggc
     7261 ggcgtgtttg tttcagcacc gtccatgttc tgaacggcgg caaacgcctc atccgtcagc
     7321 cgtccttttt catctgggtc gcccgaaagc agggcgcgtt ccagcaaagc ataaatatcg
     7381 agtttcatct caagtccgcc gtgttcggaa aacaaatatt atagcgtttt aaaaaaaaca
     7441 agatgaggca tataatctcc gcgattcggc attccgcgcc caaaccgtca aatatagtgg
     7501 attaacaaaa accggtac
//
LOCUS       DAXHMX010000100         7446 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHMX010000100 DAXHMX010000000
VERSION     DAXHMX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.100"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            28..2043
                     /locus_tag="ACFM4S_001540"
     CDS             28..2043
                     /locus_tag="ACFM4S_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6051189.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDTEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSESEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPRHIGLQGGSNGGLITAAAFVCEPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRWLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLHETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(2157..3113)
                     /locus_tag="ACFM4S_001541"
     CDS             complement(2157..3113)
                     /locus_tag="ACFM4S_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6051190.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            3349..4314
                     /locus_tag="ACFM4S_001542"
     CDS             3349..4314
                     /locus_tag="ACFM4S_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6051191.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
     gene            complement(4621..4773)
                     /locus_tag="ACFM4S_001543"
     CDS             complement(4621..4773)
                     /locus_tag="ACFM4S_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051192.1"
                     /translation="MRFVVLASFSGRSCNESVIDFDVSLRLTALSLTLSHRERGRVAV
                     ASLFAA"
     gene            4804..5127
                     /locus_tag="ACFM4S_001544"
     CDS             4804..5127
                     /locus_tag="ACFM4S_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6051193.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            5241..7418
                     /gene="tdfF"
                     /locus_tag="ACFM4S_001545"
     CDS             5241..7418
                     /gene="tdfF"
                     /locus_tag="ACFM4S_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC6051194.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         1674 a         2246 c         2137 g         1389 t
ORIGIN      
        1 agacctgccc aaccctcaag gaccccgatg aaatcctacc ccgaccccta ccgccatttt
       61 gaaaacctcg attccgccga aacgcaaaac ttcgctgctg aagcgaatgc cgaaacgcgc
      121 gcgcgttttt taaacaacga caaggcacgc gcattgtctg acggcatttt ggcgcagttg
      181 caggacacgc ggcaaattcc gttttgtcag gaacaccgcg cgcggatgta ccatttccat
      241 caggacacgg aatatccgaa gggcgtgtac cgcgtgtgta cggcggcgac ttaccgttcc
      301 ggctatcctg agtggaaaat cctgttttcg gtggcggatt tcgacgaatt gctcggcgac
      361 gatgtgtatt tgggcggcgt gtcgcacttg gtggaacagc ccaaccgcgc gttgttaaca
      421 ctgagcaaat cgggcggcga tacggcgtac acgctggaag tggatttgga agcaggggaa
      481 ttggtagaag gcggttttca ctttccggca ggtaaaaacc atgtgtcgtg gcgcgatgaa
      541 aacagcgtat gggtgtgtcc ggcttgggac gaacgccagt tgaccgaatc gggctatccg
      601 cgcgaggtgt ggctggtgga acgcggcaaa agtttcgagg aaagcctgcc tgtgtatcaa
      661 atcgataaag atgcaatgat ggtgaacgcg tggcgttatc tcgatccgca gggttcgccg
      721 attgatttga ttgaagcgtc ggacggtttt tacaccaaaa cctatttgca ggtgtcgtcg
      781 gaaagcgagg cgaaaccgtt aaacctgccc aacgattgcg acgtggccgg ctatctggcc
      841 ggacatcttt tgctgacgtt acgtaaagac tggaaccgcg cgaaccaaag ctatccgagc
      901 ggcgcattgg tggcggtgaa gctgaatcgg ggcgaactcg gggcggcaca gcttttgttt
      961 gcgcccgatg aaacgcaggc attggaaagc gtggaaacga ccaagcgttt tgtggtggcg
     1021 agcctgctgg aaaacgtaca aggccgtctg aaagcgtggc ggtttgccga tggcaaatgg
     1081 caggaagctg agttgccgcg cctgccttcg ggcgcgttgg aaatgaccga tcagccttgg
     1141 ggcggcgacg tggtttacct tgccgccagc gatttcacca cgccgctgac gctgtttgcg
     1201 ctggatttga acgtgatgga actgaccgtc atgcgccgcc agccgcagca gtttgattca
     1261 gacggcatta acgtgcagca gttttggacg acttcggctg acggcgagcg cattccttat
     1321 ttccacgtcg gcaaaaacgc cgcgcccgac atgccgacgc tggtctatgc ctacggcggt
     1381 ttcggcattc ccgaattgcc gcattatctg ggcagcattg gcaaatattg gctggaagag
     1441 ggcaatgcct ttgtattggc gaacatccgc ggcggcggcg agttcggccc gcgctggcat
     1501 caggcggcgc agggcatcag caaacataaa agcgttgatg atttattggc agtcgtgcgc
     1561 gatttgtccg aacgcggcat gagttcgccc agacacatcg gtttgcaggg cggcagcaac
     1621 ggcggcctga ttaccgccgc tgccttcgtg tgcgaaccgc aaagcatcgg cgcgctggtg
     1681 tgcgaagtac cgctgaccga catgatccgt tatccgctat tgtccgccgg ttcaagttgg
     1741 acggacgaat acggcaatcc gcaaaaatac gaagtctgca aacgctggct gggcgaattg
     1801 tcgccgtatc acaatctttc agacggcatc gattatccgc ctgcgctcat taccaccagc
     1861 ctcagcgacg accgcgtcca tcctgcccac gcgctcaagt tttacgccaa actgcatgaa
     1921 acctcggcgc aatcttggct ctactcgcct gacggcggcg gccataccgg caacggcacc
     1981 caacgcgaat ccgccgacga actcgcctgc gtcttgctgt ttttgaaaga gtttttgggc
     2041 taagggcggg gagcggcact gccgccgcga atgaaaaaag gtcgtctgaa actgcttttt
     2101 cagacgacct tttttaatgg ttgtttcaaa tcaaaatatc tatgccgccg gccccatcag
     2161 cacttcttca catccgaagg caaaaatccg taatgccgtc tgaacgcttc gttgaaccgt
     2221 cccgcgtggc ggtagccgca aaagtgcatg gcggcttgga cggtgctgcc ggattcgatg
     2281 agggcgagcg cgtgttccag ccgcaggcgg cgcaggcatc cggcgacggt ttcaccggtt
     2341 tgcgctttga aatagcgttt caggtagcat tcgttcagtc cgacgcggcg ggcgatttcg
     2401 gcgatggtca gcggacgggc gaattcgtgt tgcaggatgt cggcggcttc gtctatgcgc
     2461 cggcggcggt aaccgttgtc gtggcggcgg aaggtgaagc gcaataatcg ggcggagagt
     2521 tccagcgcgg cggcttcgtc ggcaagcagg ccgaagccgt ccgattcgaa cgggcgttgc
     2581 agcagtgggc aggccgccgc cgtcagtgcc gctgcgtttt gcgccagccg ttgcagggcg
     2641 aatcggccta ttgtttgcgg cgaaaacagg cgttcgtcca gcaagccttc gtcgtgccag
     2701 cggcgcagtt tttccagcga aaaatccaaa tgcagcgcgc acatgccgct gttgtcgggc
     2761 agcagggttt cggatacgtc cgccaactcg ccgcgtacca gccagatttc gccggcagac
     2821 gggcggtatt ccctgccgcc catttgtaac cggttctgcc ccgacaccat gacgaacaag
     2881 gcgcagttgt ggctgaaatt gtggatttcg gtgggaaacg cgcccgttcc gccgccgcgc
     2941 atccgcgaca aggtgatgcc cgagtcgaag cggttgatgc acatttccag atgcaaaccg
     3001 ggctgttttg cctgcgcaat gagcgcgctg tcggaacagc cgtccaacgc ccagccggat
     3061 ttatcggagc ggacataggt ttggtactgg cggtagatgg cggcggtgtt catgattgga
     3121 taggaacgag ttgtctaaca aatgaattaa ataggaatta ttaccaataa tcaagcgcag
     3181 ggattggttg aaacggaaaa ggtcgtctga aagggtgttt cagacgacct tttccgtatc
     3241 gggaatttgt tttgccgtat cgggaatttt gcgttttgcg gcgtggtttc tgcaggttgt
     3301 ttgcttaata ataaacattc ttattcgtat gcaaaggaac cgcacaccgt gaaaccgcgt
     3361 ttttattggg cagcctgcgc cgtcctgctg accgcctgtt cgcccgaacc tgccgccgaa
     3421 aaaactgtat ccgccgcatc cgcatctgcc gccacactga ccgtgccgac cgcgcggggc
     3481 gatgccgttg tgccgaagaa tcccgaacgc gtcgccgtgt acgactgggc ggcgttggat
     3541 acgctgaccg aattgggcgt gaatgtgggc gcaaccaccg cgccggtgcg cgtggattat
     3601 ttgcagcctg catttgacaa ggcggcaacg gtggggacgc tgttcgagcc cgattacgaa
     3661 gccctgcacc gctacaatcc tcagcttgtc attaccggcg ggccgggcgc ggaagcgtat
     3721 gaacagttgg cgaaaaacgc gaccaccata gatctgacgg tggacaacgg caatatccgc
     3781 accagcggcg aaaagcagat ggagaccttg gcgcggattt tcggcaagga agcgcgcgcg
     3841 gcggaattga aggcgcagat tgacgcgctg ttcgcccaaa cgcgcgaagc cgccaaaggc
     3901 aaaggacgcg ggctggtgct gtcggttacg ggcaacaagg tgtccgcctt cggcacgcag
     3961 tcgcggttgg caagttggat acacggcgac atcggcctac cgcctgtaga cgaatcttta
     4021 cgcaacgagg ggcacgggca gcctgtttcc ttcgaataca tcaaagagaa aaaccccgat
     4081 tggattttca tcatcgaccg taccgccgcc atcgggcagg aagggccggc ggctgtcgaa
     4141 gtattggata acgcgctggt acgcggcacg aacgcttgga agcgcaagca aatcatcgtc
     4201 atgcctgccg cgaactacat tgtcgcgggc ggctcgcggc agttgattca ggcggcggag
     4261 cagttgaagg cggcgtttga aaaggcagaa cccgttgcgg cggggaaaga gtagggagtc
     4321 gtctgaaaac ggagcttccg aaggaagcgg ggggtttctg cgaagctaaa gtgcggtttc
     4381 aacgaattga aaagcagcct gtattttgaa aataccgctc aagcaaacct acggtttgcc
     4441 gccctctccc tagccctctc ccacagggag aggggattgg gttgcaggct gcctttaagg
     4501 tttaggcaaa tttttaactt cgttgaagct ccgttttcag acgacctttt ggaacaccac
     4561 aggcacacgc atcgaacggc tgaatcaaag attcagaccg atggcagtcc gcacccgagt
     4621 ttatgcggca aacagcgagg ctacggcaac ccgccccctc tccctgtggg agagggttag
     4681 ggagagggcg gtaagccgca ggcttacatc aaagtcgata acgctttcgt tacaactccg
     4741 cccactgaac gaagccaaaa cgacaaaccg catcgtaaac cacccaaccc ataggagaac
     4801 cccatgcaaa acgaaaccat caacctgaaa cagcaccttg ccgccatcaa agaatactgg
     4861 cagcccgaaa tcatcaaccg ccacgggttc caattccgtt tggtcaaact tttgggcgat
     4921 tacggctggc atacgcacgg atacagcgac aaagtgctgt ttgccgtgga gggcgacatg
     4981 gcggtggact tcgccgacgg cggcagcatg acgatacgcg agggcgagat ggcggtcgtg
     5041 ccgaagtcgg tgtcgcaccg cccgcgttcg gaaaacggct gctcggtggt gctgattgag
     5101 ttgtccgacc cgtccgaggc cgtctgaaaa cgaagtttcc gaaggaagct gagtttctgc
     5161 gaagctaaaa gcagcctgca ccttcaatca atatgccgaa aatacaaccc caccgcacac
     5221 caacacacaa aggaaatccc atgacacgct tcaaatattc cctgctgttt gccgccctgt
     5281 tgcccgtgta cgcgcaggcc gatgtttctg tttcagacga ccccaaaccg caggaaagca
     5341 ctgaattgcc gaccatcacc gttaccgccg accgcaccgc gagttccaac gacggctaca
     5401 ctgtttccgg cacgcacacc ccgctcgggc tgcccatgac cctgcgcgaa atcccgcaga
     5461 gcgtcagcgt catcacatcg caacaaatgc gcgaccaaaa catcaaagcg ctcgaccgcg
     5521 ccctgttgca ggcgaccggc accagccgcc agatttacgg ctccgaccgc gcgggctaca
     5581 actacctgtt cgcgcgcggc agccgcatcg ccaactacca aatcaacggc atccccgttg
     5641 ccgacgcgct ggccgatacg ggcaatgcca acaccgccgc ctatgagcgc gtagaagtcg
     5701 tgcgcggcgt ggcggggctg ctggacggca cgggcgagcc ttccgccacc gtcaatctgg
     5761 tgcgcaaacg cccgacccgc aagccattgt ttgaagtccg cgccgaagcg ggcaaccgca
     5821 aacatttcgg gctgggcgcg gacgtatcgg gcagcctgaa tgccgaaggc acgctgcgcg
     5881 gccgcctggt ttccaccttc ggacgcggcg actcgtggcg gcagcgcgaa cgcagccgcg
     5941 atgccgaact ctacggcatt ttggaatacg acatcgcacc gcaaacccgc gtccacgcag
     6001 gcatggacta ccagcaggcg aaagaaaccg ccgacgcgcc gctcagctac gccgtgtacg
     6061 acagccaagg ttatgccacc gccttcggcc cgaaagacaa ccccgccaca aattgggcga
     6121 acagccgcca ccgtgcgctc aacctgttcg ccggcatcga acaccgcttc aaccaagact
     6181 ggaaactcaa agccgaatac gactacaccc gcagccgctt ccgccagccc tacggcgtag
     6241 caggcgtgct ttccatcgac cacaacaccg ccgccaccga cctgattccc ggttattggc
     6301 acgccgaccc gcgcacccac agcgccagcg tgtcattgat cggcaaatac cgcctgttcg
     6361 gccgcgaaca cgatttaatc gcgggtatca acggttacaa atacgccagc aacaaatacg
     6421 gcgaacgcag catcatcccc aacgccattc ccaacgccta cgaattttcc cgcacgggcg
     6481 cctatccgca gccatcatcg tttgcccaaa ccatcccgca atacggcacc aggcggcaaa
     6541 tcggcggcta tctcgccacc cgtttccgcg ccgccgacaa cctttcgttg atactcggcg
     6601 gcagatacag ccgttaccgc accggcagct acgacagccg cacacaaggc atgacctatg
     6661 tgtccgccaa ccgtttcacc ccctacacag gcatcgtgtt cgacctgacc ggcaacctgt
     6721 cgctttacgg ctcgtacagc agcctgttcg tcccgcaatc gcaaaaagac gaacacggca
     6781 gctacctgaa acccgtaacc ggcaacaatc tggaagccgg catcaaaggc gaatggcttg
     6841 aaggccgtct gaacgcatcc gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg
     6901 cagcaggacg cgacccgagc ggcaacacct actaccgcgc cgccaaccaa gccaaaaccc
     6961 acggctggga aatcgaagtc ggcggccgca tcacgcccga atggcagata caggcaggtt
     7021 acagccaaag caaaacccgc gaccaagacg gcagccgcct gaaccccgac agcgtacccg
     7081 aacgcagctt caaactcttc actgcctacc actttgcccc cgaagccccc agcggctgga
     7141 ccatcggcgc aggcgtgcgc tggcagagcg aaacccacac cgaccctgcc acgctccgca
     7201 tccccaaccc cgccgccaaa gcccgcgccg ccgacaacag ccgccaaaaa gcctacgccg
     7261 tcgccgacat catggcgcgt taccgcttca atccgcgcgc cgaactgtcg ctgaacgtgg
     7321 acaacctgtt caacaaacac taccgcaccc agcctgaccg ccacagttac ggcgcactgc
     7381 ggacagtgaa cgcggcgttt acctatcggt ttaaataagg tcgtctgaaa acggagtttc
     7441 tgcgaa
//
LOCUS       DAXHMX010000101         7443 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHMX010000101 DAXHMX010000000
VERSION     DAXHMX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.101"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="ACFM4S_001546"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFM4S_001546"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="ACFM4S_001547"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="ACFM4S_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6051195.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="ACFM4S_001548"
     CDS             complement(1737..2630)
                     /locus_tag="ACFM4S_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6051196.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYFDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="ACFM4S_001549"
     CDS             2972..3421
                     /locus_tag="ACFM4S_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6051197.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="ACFM4S_001550"
     CDS             3495..3803
                     /locus_tag="ACFM4S_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051198.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3919..4761
                     /locus_tag="ACFM4S_001551"
     CDS             3919..4761
                     /locus_tag="ACFM4S_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6051199.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            4751..5497
                     /locus_tag="ACFM4S_001552"
     CDS             4751..5497
                     /locus_tag="ACFM4S_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6051200.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNHSKYTPSPAAFIRQYRYDVTDDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5519..6106
                     /locus_tag="ACFM4S_001553"
     CDS             5519..6106
                     /locus_tag="ACFM4S_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6051201.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTQEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6112..6291
                     /locus_tag="ACFM4S_001554"
     CDS             6112..6291
                     /locus_tag="ACFM4S_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6051202.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6532..6972
                     /locus_tag="ACFM4S_001555"
     CDS             6532..6972
                     /locus_tag="ACFM4S_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6051203.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1910 a         1976 c         1680 g         1877 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcgtgtcgga tgcacaccgg acgggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttaccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acggttcaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aaatcgccat ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtacg caagtctttg atacgtgagg aatccaaaac atacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcgttg
      721 cgtttgcgca tttccaattc atacagtttg gatttcaaca tttccatcgc ggccgctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atacgcacgg cggagtcggt tttgttgatg tgctgaccgc ccgcgcccga tgcgcgatag
      901 gtgtcgatac gcaaatcggc ggggttgatt tcgatttcaa tggaatcatc gatttcaggg
      961 taaacgaaca cggaggcaaa cgaagtatgg cgtttgttgt tcgagtcaaa cggtgaatag
     1021 cgcaccaagc ggtgaacgcc ggtttcggta cgcagcaaac cataggcgta ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatac ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc cgcgcctgcg gtgatgtcga taaagcagtt gttcgggtcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgtcgcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgacaac ttcttccaat cggtctttct tgccctgata atccatataa
     1561 acgcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tggaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaaccgga tggcgcgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgaa ataagagaag aaaccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atccgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cgggcgacat acccgatgca aaaaggctgc cgactatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaatacca ttttctttca aaaccttaat aatgcctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcagcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atgggggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggcggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcccttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttctga aatcaacgat caattcccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tgctgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccggcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccacc ccttcaaacc aagccgcgtt tttcagtaaa
     3841 atgcggcttt tttaacggca aacaaagatt ttttaacaag cacatcattc ttttgtgcca
     3901 tccgaaccgg gtaaaaatat gattcaacac gcaggcttgg gctaccgccg cgacttggcg
     3961 gaagactttc tctcgctttc cgaaaacagc ccgatatgct ttatcgaagc cgcaccggaa
     4021 aactggctga aaatgggcgg ctgggcgcgc aaacagtttg accgtgtggc ggaacggctg
     4081 ccgctggcgt tgcacggatt gtctatgtcg ctgggcgggc aagcaccgct ggatactgat
     4141 ttgatagacg gcatcaaaga aatgatgcgc cgttacgatt gcacgttttt ctccgaccat
     4201 ttgagctact gccacgacgg cggtcatctt tacgatttgt tgccgctgcc cttcaccgag
     4261 gaaatggtgc atcatacggc gcggcgtatc cgtgaagtgc aagaccgttt gggctgccgc
     4321 atcgccgtgg aaaacacgtc ctactatctg cattccccgc ttgccgagat gaacgaggtc
     4381 gagttcctca acgccgtcgc acgtgaggcc gattgcggca ttcatctgga tgtgaacaat
     4441 atctacgtca acgccgtcaa tcacggcctg ttgtcgccgg aggctttttt gaaaaacgtt
     4501 gacgcagatc gcgtgtgcta tatccatatt gccggacatg acgtggaaac gccggaattg
     4561 ttgattgata cacatggcgc ggcagttttg ccgactgttt gggacttgct cgaacttgcc
     4621 tatgccaagc tgccgacgat tccgcccacc ctgttggaac gcgattttaa tttcccgcct
     4681 tttgccgaac tcgaagccga agtcgccaaa atcgccgatt atcaaacgcg tgccggaaag
     4741 gaatgccgcc gtgcagcctg aaacctccgc ccaataccag caccgtttcg cccaagccat
     4801 acgcgggggc gaagccgcag acggtctgcc gcaagaccga ctgaacgtct atatccgcct
     4861 gatacgcaac aatatccaca gctttatcga ccgctgctat accgaaacgc ggcaatactt
     4921 tgacagcgaa gaatggagcc gtctgaaaga aggtttcgtc cgcgacgcgc acgcccaaac
     4981 gccctatttt caagaaatcc ccggcgagtt cctacaatat tgccaaagcc tgccgctttc
     5041 agacggcatt ttggcactga tggattttga atatacccaa ttactggcag aagttgcaca
     5101 aattccggat attcccgaca ttcattattc aaatcacagc aaatacacgc cctcccctgc
     5161 ggcctttatc cggcaatatc gatatgatgt taccgatgat ttgcaggaag cggaaacagc
     5221 cttgttaata tggcgaaacg ccgaagatga tgtgatgtac caaacattgg acggcttcga
     5281 tatgatgctg ctggagataa tgggttcctc cgcgctttcg tttgacaccc tcgcccaaac
     5341 ccttgtcgaa tttatgcctg aggacgataa ttggaaaaat attttgcttg ggaaatggtc
     5401 aggctggact gaacaaagga ttatcatccc ctccttgtcc gccatatccg aaaatatgga
     5461 aggcaattcc ccgggccaaa accatctatc cgcataaaat taccttgttc ccgatactat
     5521 gccgctaccc gacctgaccg atgccgaatt aatagagtcg cgtaaactgc ttctgcattt
     5581 tgcgcggctt cagttgcccg accaccctga tttggctgaa gatttagtgc aggaaacatt
     5641 gctgtccgca tacagcgcag gcgacagttt tcaaggcagg gcacttgtca acagctggct
     5701 ttttgccata ttgaaaaaca aaattattga cgcattacgt caaatcggaa ggcagaggaa
     5761 agtctttacc acactggatg acgagctact ggatgaagca tttgaaagcc atttttccca
     5821 aaacgggcat tggacgcagg aagggcagcc gcaacattgg aacactccgg aaaaatcatt
     5881 aaacaacaac gaattccaaa aaattctgca aagctgccta tacaacctgc ctgaaaacac
     5941 cgcacgggta tttaccctga aggaaatact cggtttttca tccgacgaaa tacaacaaat
     6001 gtgcggtatc agcacgtcca actaccacac cattatgcac cgcgcccgag aatcattgcg
     6061 ccaatgcctg caaatcaaat ggttcaacca agaaaacccg aagtaaacgt tatgaaaaaa
     6121 tgccgcgata tcgccctgct tctttccaaa catcaggacc gggaaaccac cccgggcgag
     6181 aagatttcca tatacacaca cctgctgttc tgtccgtatt gccgtgaata taaaagacaa
     6241 cttcaaacca tcaaaagatc actggcaaaa acaaccagaa cttcaaaata aatgccgtct
     6301 gaaaaggctt cagacggcat aagctgacgg aaacaaatca aaccgattta ctgttatctg
     6361 cagttcatcc ataatacaca cttcaaaagc agcatatttc cccatacgga atgtataaat
     6421 acgcaaaata cgaaggctgc atcaatttgc catatttgct ttatttgcct tatttcacag
     6481 acgacactcc ccctcccgcc caacccgttc tttctgaatg agcagatttc aatgattaag
     6541 gaaaccctaa tgcgcccaat cttcctatct ttcgttttac tccctatttt gataaccgcc
     6601 tgcagcacac cggacaagtc tgcccgatgg gaaaatatcg gcacaatctc aaacggcaat
     6661 attcatacat atatcaacaa agacagcgtg agaaaaaacg gaaatctgat gattttccaa
     6721 gataaaaaag ttgtgaccaa tctaaaacaa gaacgttttg ccaacacccc cgcatacaag
     6781 actgccattg ccgagtggga aatccactgc aacaacaaaa cataccgctt aagttcgcta
     6841 cagttgtttg atacaaaaaa cacggaaatt tccacacaaa gctacacagc ctcttccctc
     6901 cgcccgatga gcatcctgtc cggaacatta actgaaaaac aatatgaaac cgtatgcggg
     6961 aaaaaactct gattacaact tatgcacaaa cttatccata aaccttatta taaaaatgcc
     7021 gtctgaaata ctgaaatatc agcatttcgg acggcatttt gccatttccc cgaaaagtta
     7081 cccccaaagt tatccacatt attttttaaa ccagcttcca tccgaaatat agtggattaa
     7141 caaaaatcaa gacaaggcga cgaagccgca gacagtacaa atagtacgaa accgattcac
     7201 ttggtgcttg agcaccttag agaatcgttc tctttgagct aaggcgaggt aacgccgtac
     7261 tggtttttgt taatccacta taaactcgct atacaatttc actatccaaa cgtaaattgt
     7321 tccattgata cacaaaactg cttgccccca taattttgat aaagcatttc ttacattccc
     7381 ggctccgtcc cgtaaccaac acagcctcta aaaaataatc gcttgttttg acaccgatac
     7441 act
//
LOCUS       DAXHMX010000102         7351 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHMX010000102 DAXHMX010000000
VERSION     DAXHMX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.102"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            217..429
                     /locus_tag="ACFM4S_001556"
     CDS             217..429
                     /locus_tag="ACFM4S_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6051204.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            577..2160
                     /locus_tag="ACFM4S_001557"
     CDS             577..2160
                     /locus_tag="ACFM4S_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6051205.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDADLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLTF
                     DRMKINSQLLLQTILGRFSVSSDNAGKQFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2242..3339
                     /locus_tag="ACFM4S_001558"
     CDS             2242..3339
                     /locus_tag="ACFM4S_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6051206.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3453..4568
                     /locus_tag="ACFM4S_001559"
     CDS             3453..4568
                     /locus_tag="ACFM4S_001559"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6051207.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4693..5148
                     /locus_tag="ACFM4S_001560"
     CDS             4693..5148
                     /locus_tag="ACFM4S_001560"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6051208.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKICQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5219..5695
                     /locus_tag="ACFM4S_001561"
     CDS             5219..5695
                     /locus_tag="ACFM4S_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6051209.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5683..6609
                     /locus_tag="ACFM4S_001562"
     CDS             5683..6609
                     /locus_tag="ACFM4S_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6051210.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6674..7231
                     /locus_tag="ACFM4S_001563"
     CDS             6674..7231
                     /locus_tag="ACFM4S_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6051211.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         1926 a         1811 c         1913 g         1701 t
ORIGIN      
        1 tgttaatcca ctataaatgc gccgattgcc aaggcgatga tgctcaacca gggtttttgc
       61 gaagcgttca ttgttgtcgt cctgatggga attgccgcca ttatgcgcaa gcgtccgtct
      121 ttttccaaac cgccgcaccg tttccgcgtt tgcccaagtt cacgccccga cttacaataa
      181 cgcccgtcag attgcagttc caacgaaagg aaaatgatgg aaacccttat cctcgacatc
      241 ggcggcatga gctgcggcgg ctgcgtcaaa agcgttaccc ggatattgga aggcgtaaaa
      301 ggcgtggcaa gcgtcgaagt cagtcttgaa aacaaaagtg cgaccgtcgg atacgacccc
      361 gcgcaaaccg atgcaggggc gttgattgaa gccgttgaag acggcggcta tgatgccgcg
      421 ttgaaataaa gcggcaaaaa tgccgtctga agccttcgcg cctcccagat gccgtctgaa
      481 gtacgctccg cgccttcaga tggcatttga tttagttgaa cgggtaggat gaattggtta
      541 aacttgcggg caatagtgcc ttcagacaaa caaaatatgc cgaacaaaaa gaaaaaagcc
      601 gcctttgcgc gtgctgcaac ggagaacggt gtgccggacg tgtccaacat caaaaattca
      661 tatgatgatt tgatgtatga atccaaagca ttttcccaaa ccgccatcaa cgccttggag
      721 gctcgcgccc gcatgatggg tttgcagccc gcgcccgccg ccaatgccaa ggtcttggag
      781 ctgggctgtt cgatgggcgg caacatcatc acgcaggctc tttattaccc ggatgcggaa
      841 tttgtcggta tcgacttgtc cggcaggcag gttgcgcagg gcaacgccat cattgaaaaa
      901 atgggcttga aaaatgtgcg tctggaagaa aaagatattt tgaccgtcga tgagtcattc
      961 gggaagtttg actatatcat cgtccacggc atttggtcgt gggtgcctga cgcagttaaa
     1021 gacaaaattt tttcgatttg ccggaacaac ttgaccgaac acggcattgc ctatatttca
     1081 tacaatgttt accccggctg gaaacggcag gagcagttgc gcgaaattat gtactttgcc
     1141 ggcagggatg tgcttgaaga acccttggaa gcacggacgc ggaaaggctt ggacgcgctc
     1201 aaggcgctgg cggaaatttt agaaaacgac aagggcttgg acggcggcgg caaacttccg
     1261 gcgattcaaa aaatattgaa tcataatttt tattacatcg cacacgaata tatggaggcg
     1321 tttaacgacc cgatttacgt caacggcttc atcgaatggg caaaccgcca ccggctggcg
     1381 tatatcgggg atgccgattt gcacgcgtct tttgtttctt ggatggcgga gcatacgcgg
     1441 gagcggattc tggcattggc agggggcgat tatattgcca aagaatttta cagcgatatt
     1501 ttatccgacc gccaattccg ccgttcgctt ttatgccgtg aggaagtcgg ggatactgtc
     1561 aggcgtgacg agtcggttgc cgtcgaagtg atagaaagtt tgaatttccg cccggcaaga
     1621 ggggaaacaa tcaactttga tgaaaacgat accctgcttt cgggcatacg cgatgtgatg
     1681 aaaaccggag agccgtttaa aacggaagat gttgcggaaa atctcgcccg ccgatttccc
     1741 ggtttgacat tcgaccgtat gaaaatcaat tcccagcttt tattgcaaac cattctcggg
     1801 cgtttttctg tttcatcaga caatgcgggc aaacaatttt ttgaagacca taaaacctat
     1861 gtgccggcgc gttttacaga ttatgccgcc gcctttgtgg aacacggcgc ggaagcgttt
     1921 gtccggcctg ccaaccgtta caacgaaagc accccctcat tcggatatgg gcatttgtac
     1981 attatgcggc aattgtcgcg gccgacgagc aaacaggcat tgattgaaac ggttgccgaa
     2041 aacttgaaca tcgtcagcac cacgcccgac ggtttgacat tccatccgcc tgccgaagtg
     2101 tatgtggaag aaatattggc agacttggca gacaggcatt ttctcgtttc ggcggattga
     2161 cgggcaagtc ccgacggcaa tccgtccgat atattttcaa acaggaaaaa cacacactac
     2221 gaaaagcggt acacgacaca tatgaaccag acatttacct tgcccgatac gcgcccgtat
     2281 ccccaaaatc cgattaagaa ccacctgctg ctcaatgcct accagttggc gcataattct
     2341 tcccaggctt cgcgcaaact ctcgtccggc caacttcaaa ccgaaatcag ggggatgctt
     2401 gagcaaaacc actatatcaa cctttccctc gcgttgacga tgtcgcccga tgccggaact
     2461 tatgccgcgc tgctttccag tgtgaacgcg gtactcgatt gcgagaaaga aggcgaagtg
     2521 cagtggttcg ccctgccggt cgtgctggtg tccggctgca agaaagaacg ggcaatcgag
     2581 atgaagctgc cgacggaggc attgtttgcc tgcctgcaaa actatccgca cctgcgcgcg
     2641 ttgacgcaag agacacaatg gctgccttat cttgtgcatt cgtccgattt gagtgcggtc
     2701 gcgccggatg agtggtggcg tgccaaacaa aataccgaag cggcggcgca acacttacgc
     2761 cgtttcgccc cgcgcccttt gctgttgccc gaagggcagt ccgtccacgt cgtttacgcg
     2821 ctgggtttcg gcagcggcaa ggttcagacg gcattgggtc agaaccttct tcaggcaggc
     2881 ctgcccttaa tgcaggtatg gcaggaaaat cttgcatcgg aaggtgttac gctgtttgcc
     2941 aatccacttt ccccggattc tccggtacgc gcgctttcag acggcagcca cacgcgccag
     3001 cgtatggcga tggatgtttt tgcggcaaac gcgatacgcg ccgtccgtat gcagagtccg
     3061 cgcgtcggcg tggtcgctgc ggcaaaggcg ggcggacagg ttttattcgg gtttaatgcg
     3121 accgacggcg cgtttgaagt cgtgccgcag gtgttttcgt ggcagctttc ctttaccgac
     3181 aacatcgccg tcatccagca aaatttcctc gacctgatgg cggagtgccg cgtggaacac
     3241 gtttacctgt tgcacaatcc cttgggcgaa caggaaagca tcccgagcta tgcggaagca
     3301 ttgaaacggg aagggcacaa tccgtttttc agcgcatagt gatttgtagt agtgataggc
     3361 tttctcattt atataataaa tcaaaataaa gagcacaaca ctttttcatt ctgtgttgtg
     3421 ccttgagtga aacgaaagga tgaattataa atatgaagat tgtaattgca ccggattcgt
     3481 ttaaggaaag cttgacagct caacaggtag ctgaagcaat aaaaagaggc ttccaacaat
     3541 cgatagcaga tgtggaatgt ctcctctgtc ctgtcggtga tgggggagaa ggcactgtag
     3601 atgctatccg acattctctt gacctagaag aaaaatgtct tcaagtgaca ggaccttttg
     3661 gacaaaaaga agtcatgcgc tattttcaaa aagaacaact agccctattc gaagttgctg
     3721 atttggttgg tcttggaaaa atcccgctag agaaacgaaa tccattacaa atccaaactc
     3781 gtggtattgg agaattgatt cgccacctca ttagtcaaga aattaaagaa atctatattg
     3841 gcgttggcgg tacggccagt aatgatggag gtattgggat tgctgctggt ttaggttatc
     3901 aattttatga tgaggatgga aataccttac ccgtttgcgg tcaatcctta ctaaacctag
     3961 cttctgtttc aacagaaaat cgctatgaaa ttcctgaaga tgttcacatt cgtattttag
     4021 cagatgttgt gagtccctta tgtggacacc aaggtgcgac ctatacgttt ggcaagcaaa
     4081 aagggttgga ttctactatg tttgaggccg tagatcaggc aatacaagat ttttatgaaa
     4141 aagtctcccc tgcaacatta aaacttaaag gagcaggagc tggtggaggc atcgctggtg
     4201 gtttgtgcgc ctttgctcag gcaagtatcg tatctggaat tgacacctgc ctggacttga
     4261 ttgactttga taagaaagtg tcagatgttg acttggttat cattggtgaa ggaagactag
     4321 atcgtcaaag tttagcaggg aaagcgccta ttggtgtagc aaaaagaacc cctgtcggag
     4381 ttcctgttgt cgctatttgt ggcagtctcg ttgaagattt gccttccctg ccatttgaaa
     4441 atatccaagc tgccttttct attttggaga aaagtgaacc tttagaagat agtttgaaaa
     4501 atgccagtct ctatttggag catacggctt ctaatattgg acacttatta aatatgccta
     4561 agatttagcc aaaccatttc ttccagatgg atgttttggc tggttctgcc ttatgcggtt
     4621 acgattaacc gaccgatgcc gtctgaaagg gtttccgctt cagacggcat cgcgccggaa
     4681 cagggagggg atatgaaaaa accgaaaatc ctttttgtct gcctcggcaa catctgccgt
     4741 tcgccgatgg cggaatacat tttgcgccgc cgcgccgccg aagcgggcat tccccttgaa
     4801 gcggacagcg cggggacatc gggctggcac gacggcgaag atatgcaccg cgagacggca
     4861 aagatattga aaaaacacgg tatcgatgct tcaggcttta ccagccgcaa aatctgccaa
     4921 agcgatgcgg cggcgtttga ctgcatcatc gcgatggacg gcaagaattt gtccgaattg
     4981 gaaagaacct tcggcaggcg gccggaaaaa atattcaagc tgaccgacct gatacccgaa
     5041 agcggttacg accatgtccc cgatccgtgg tatacgggcg attttgaaga aaccttcagg
     5101 cttgcggatg cgggctgtcg ggcattgttg gaaaagattt ccaaataaag caattgaata
     5161 caaatataaa accgcctcct gccgtatcgg ttgttcagac ggcataaaca gagaatttat
     5221 gaaaacaaat ttcaaacaga aaattatcga acaggcacgc agcgaaggct tgcaggtaac
     5281 cgctttgcgc gagcaggtat tagatattgt cttgcagcaa agcggcgtga ttaaagccta
     5341 caacgtcttg tcgcagatgc agcagcaaag cgagggcgtg cttgcgccgc cgaccgccta
     5401 ccgcgccctt gatttttggg cggagcaggg cgttttgcac aaagtggcgg cggtcaacgg
     5461 ctatattttg tgcagccacg cgcagcacga gtgcgacgac cattgccacg accacgaaga
     5521 agccgaagcg caccacagcg cgtttatttt ggtctgcacc gaatgcggca cggcggacga
     5581 gcaaaccctc agccacgagt gggcggcact gcgcgcaggc gttgccgaaa gcggctttgc
     5641 gctgaaagaa gaacacgttg ttttaactgg gatttgtaaa aaatgtcaga agtgaaaaaa
     5701 accaaagtcc acctgatttc aggttttctg ggaacaggca aaaccactgc gctcaaaagc
     5761 ctgatggcgc aaaaagaccc gaacgaaaaa tgggtcatca tcgtcaacga gttcggcgaa
     5821 atcggcattg acggcgcggt attgagcgac aacggtatcc ctgtggcaga aatcgccggc
     5881 ggctgtttgt gttgcaccgc cggcccgcaa atgggcgtaa ccgtgcagaa aatgctgcgc
     5941 gacgccaagc ccgaccgcct gatgattgaa gcaagcggac tggctcacgc agccagcgtc
     6001 atcgacgaac tcaaagccaa accgctggac agccttttgg aaatcggcgc cgtctttacc
     6061 gtcgtcaatc cgcgccagtt catcaacccc gattacgcgc aacaggcgtt gtataaagac
     6121 caaatcggca tctgcgacgt attggtcgcc agcaaaaccg atttatgcac caccgaacag
     6181 cttgccgaat ttcacgacaa agcggcaaaa ctgttcccgc ccaaagccaa agtggtcgaa
     6241 gtccaaaacg cacaactcga catccggtgg ctcgatattc ccgtcatcga aaaatcacgc
     6301 taccgcctca aagccctgcc ggacaacacc atgggcttcc agtcgcaagg tttcacattc
     6361 cccgccggac gcgatttcga cggcgaaaag ctgaccaact ttttcaacga cctgcccaaa
     6421 atgaccgaag gactcgtccg cgccaaaggc gtgtttcaag tattgggaac gtgggtgtgg
     6481 ctcaactggg tagacggaca gtggggcgcg aaccaagtct catggcggcg cgattcgcgt
     6541 tttgaattga ttgccaaatc gtttgatgcg gatttaatcg aacaaaaact taaagatgca
     6601 ttggaatagc gttaacgcgc tttcagacgg catatgaaat gaaaatgccg tctgaaggtc
     6661 tgaggaggat gggatgaaac ccaaatttaa aaccgtttta accgcgctgc ttttggcggt
     6721 ttccctgccg tctatggcgg caacccatgt tttgatggaa accgatatgg gcaatatccg
     6781 tttggttttg gacgagtcca aagcccccaa aaccgttgct aatttcgtgc gctatgcccg
     6841 aaaaggcttt tacgacgaca ccgtttttca ccgcgttatc gacggtttcg ttatccaagg
     6901 cggcggattt accgaagact tggtacaaaa ggcaaccgat aaagccgttg ccaacgaatc
     6961 cggcaacggc ttgaaaaaca ccgccggcac catcgccatg gcgcggacgg cagaccccga
     7021 ttccgccacc agccaattct ttatcaatct cgcggacaac gcttcgctcg actacaaaaa
     7081 cggacaatac ggctataccg ttttcggcag ggtcgaaagc ggaatggaca cagtttccaa
     7141 aatcgcccgc gtcaaaaccg ccacgcgcgg cttttatcaa aatgtacccg tacagcccgt
     7201 caaaatccgt cgcgttgttg tcggacagta acacgcagac agacgttcag acggcgtcgc
     7261 ccgtttcaca aaaaacgccg tctgaagtga aaaaatattt taaaacagac agttgatatt
     7321 gacaaattca aaccgaggat tttaaaatgc t
//
LOCUS       DAXHMX010000103         7350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHMX010000103 DAXHMX010000000
VERSION     DAXHMX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.103"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFM4S_001564"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFM4S_001564"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6051212.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLFVKINGAAADYVLEGEMLTIAGVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRATMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAVAFDYQGKRATEAVLLLA
                     EAEQTFPLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGVELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLCADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2923..3717)
                     /locus_tag="ACFM4S_001565"
     CDS             complement(2923..3717)
                     /locus_tag="ACFM4S_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6051213.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFAQAWK"
     gene            complement(3834..3968)
                     /locus_tag="ACFM4S_001566"
     CDS             complement(3834..3968)
                     /locus_tag="ACFM4S_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051214.1"
                     /translation="MPSERIFRSILRLVGVSSETVKQKFAGVELSYAGKAAGESLYCL
                     "
     gene            complement(4468..5337)
                     /locus_tag="ACFM4S_001567"
     CDS             complement(4468..5337)
                     /locus_tag="ACFM4S_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC6051215.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIRTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPGEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5388..5924)
                     /gene="ruvC"
                     /locus_tag="ACFM4S_001568"
     CDS             complement(5388..5924)
                     /gene="ruvC"
                     /locus_tag="ACFM4S_001568"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6051216.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAS
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5927..6166)
                     /locus_tag="ACFM4S_001569"
     CDS             complement(5927..6166)
                     /locus_tag="ACFM4S_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6051217.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6197..7207)
                     /gene="dusB"
                     /locus_tag="ACFM4S_001570"
     CDS             complement(6197..7207)
                     /gene="dusB"
                     /locus_tag="ACFM4S_001570"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6051218.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNAQAGSALMQNEPLVAAILEAVVRAVDVPVTLKTRLGWHDDHKNLPAVARIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
BASE COUNT         1650 a         1942 c         1942 g         1816 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggc tcgagcttgt tgcagaggtt gaacgcctgc
      241 accaggcggg cggcgacctg cgggttaaag cggtcgattt cgatgacttt gtcggcgatg
      301 aagcggtagc cgctgccgtc ttctgcgtgg aaatgcggga cgttgcggct gaagctgccg
      361 atgagcgagc gggctttgtt gggattttcg aggctgaact tcggatgctg caaggcggtt
      421 tgaacctgtt gcagggtgtc gctgcggcgg cttgagccga cgagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgag ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttaac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcgccgt atttttcggc aacggtttcg atgtgcgcgg ggtcggcgca caggacgaag
      661 gcgcggcaga cgttgcgcag cgtgcgccag ccggcagctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag gaagcggacg
      781 gcaagcgtat ccaacaaggc ttcgcgcgcc tgatggtagc gcagcgggtc gatgttttct
      841 gcgccgtccc acagttcggc ttcagacgga acgcccaaaa gcagggcttt gaaggcgttg
      901 tccaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc atgtttcggc
      961 aactcgacgc cgtctgaaag cgcggcaagg ttggcggcga cggcgcggcg gtagagcgtt
     1021 tgcgcggctt cccagcgcgt gaaggcgtcg ctgtcgtggg caagcaggag cagcaggtca
     1081 tcgtcgctgt acggatagtt cagatgcact ggcgcactga acccgcgcag cagcgaggga
     1141 acgacggctt cggttacgcc ttcgagcggg aaggtctgtt cggcttcggc cagcagcaac
     1201 acggcttcgg tcgcgcgttt gccctgataa tcaaatgcca ccgcttcgcc gttgcggttc
     1261 agcagcccga ttttgacggg aatcatcatc ggctgcttgt ccgccatatc gggcgtgggc
     1321 ggcacggttt gtttaatggt caactcgaaa acattgtttt tcagacgacc ttccgcttcc
     1381 aaaacgggcg tgcccgcctg gctgtaccac aaggcgaact ggtcgagatt aatgccgttc
     1441 gcgtccgcca ttgtcgcgcg gaaatcgtcg caggtcacgg cttgcccgtc gtggcgttgg
     1501 aaatagagct tcatgccttt ttggaagccc tcttcgccga gcagggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatag
     1621 ctggcggggc gcaccggatg ggcggtcggg cctgcgtctt cggggaactg gtgctggcgc
     1681 agcaggcgga tgttctcgat gcggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcag cccttccttc agtgaaagct ggaaccagtc gcggcaggta
     1801 acgcggttgc ccgtccagtt gtggaaatat tcgtgtccga tcacggattc gatgccttca
     1861 aaatcggtat cggtggcggt tcggctgtcg gcaaggacga acttggtgtt aaagatgttc
     1921 aaacccttgt tttccatcgc acccatattg aaatcgccta cggcgacgac catgaaaata
     1981 tccaagtcgt attccaagcc gaagcgcgtt tcgtcccact tcatcgcgtt tttcaaggat
     2041 tccacggcaa agccgacctt gggcttgtcc gcttcggtgg tgtaaaactc gattttgacg
     2101 tttctgccgc tcatggtggt gaaacggtct tccgttaccg ccaaatcgcc cgcgaccaaa
     2161 gcaaacaaat agctcggttt ggcaaacggg tcttcccatt tcacccaatg gcggccgtct
     2221 gaacactcgc cgccgtcgat tttgttgccg ttggagagca aaacgggata gcgttttttg
     2281 tccgcaacga tggtggtcgt gaatttggac atcacatccg gacggtcgat gtagaacgtg
     2341 attttgcgga agccctccgg ctcgcattgg gtaaacagat tgccaccgga agcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atctcggttt ccacttcgac ggtgaagcgt
     2461 tcggacggca cgcctgcaat cgtcagcatc tcgccttcca acacataatc cgctgccgcc
     2521 ccattgattt tgacgaacaa gagtttcgcc gaaccgtcca acaccagcgg ctctcctacc
     2581 ctctgcggct cgaccgtcaa acgtgatttc acgacggttt gcggttcggc aatgtcaaaa
     2641 tgcaggtcgg tttcaaggat gcggtaggca ggcgtttggt aatctttcag ataacggacg
     2701 gttttgctca tttttttctt tcaatattgt ttggtttgac tggaaaaggc ttcagacagc
     2761 acggatacag cttttaaagc ggattaacaa aaatcaggac aaggcgacga agccacagac
     2821 agtacagata gtacgggctg taccggtttg aatttaatcc accatatgcc gtctgaaacc
     2881 gaaacggcgg cacaagcgta ccgccttcgg acagcaggtt ttctatttcc acgcttgggc
     2941 aaattctgac caatgcggtt tatcttctgt ttgggcggaa agatagtttc tgatccgctt
     3001 gatttccatt tcgtattcgt ccgcgtcgaa tctgccgctg accagacaaa aacgcagata
     3061 catcaaatac gtattcacgg catcggtttc gcaataattg cggatttctt tcagcctgcc
     3121 cgcgtggaac gcatcccaca ccttgctgcc gtccataccc agcttgcccg gaaaaccgca
     3181 cagtttcgcc atatcgtcca gcggcacgtt tgccctcggc tggtaaagcg cgagcaaatc
     3241 catcaaatcg cagtggcgtt ggtgataacg gctgatgtag ttgttccact tgaaatcgcg
     3301 gctgtcgccg aaatcgccgt cgcccatatc ccaatagcgc gcggcgttaa tgccgtatat
     3361 cagggagcgg taatgcagaa ccggcaggtc gaaaccgccg ccgttccaac tgaccagttg
     3421 cggcgtatgt ttttcaatca actcgaaaaa tttggcaatc acgacttctt cgccatcgtc
     3481 catctcgccg atggtgccga catggacttt atcctgcccc caacgcatgc agcacgaaat
     3541 cgccacaatc tgatgaagat gatgctgcat aaaatcccca cccgtctgag cacggcgttt
     3601 ttgctgggca aacagcacca cttcatcgtc gggcagcgag gacggcagct cgtacaatgt
     3661 tcggataccc tgcacatcgg gtacggtttc aatatcgaaa gccaaaatcg tattcatggc
     3721 agtaccttgc attcaaaaac agacttgcgc ctattgtgtc attaaaaggc ggataaaaaa
     3781 agaggcaacc ccccacagga ttgccccaat acctcaaatc agagatttac gcttcacaaa
     3841 caatacaggc tttcgcctgc ggctttgccc gcgtagctca actctacgcc ggcaaacttt
     3901 tgtttcaccg tttccgatga aaccccgacc aatcgcaaga ttgaccggaa aatcctttca
     3961 gacggcattt cctgcctatc gtgtaattcc atgtagcgaa atgtacgcca ttttctacgc
     4021 tttgccaagc attttttaca atataaatgt caaaacatta attttataaa attgctgaaa
     4081 atattaaata tatgaatttt tattttaaat attttaataa atataaattt aattttgatt
     4141 tatatttaaa tttaagcata aaatgttaaa tattaaaggc aatatgaaag gcatatatta
     4201 aatatttatt tataacgcta tgtttttaaa gaaaattaat tttaatatat taactagatt
     4261 gtctgcagat attcatgggt ttgcggtatt tcttccaaaa cctgcttcga attttccgac
     4321 caagtcttaa aaatattgtt ttttgagata cttaaatagc agcgattatc aaatgaaatc
     4381 tgttcatata atctgccatt tcgcatttaa aaaaacaaac aggagtttcg actcgaaacg
     4441 cctgatatgt tttgtaattt tacgtagtca gtaaaaatcg gggctgcctt ccggacgggt
     4501 tttaaaacgc ttgtgcagcc aaaaatattg ttctggatgt tcgcgcaccc gctcttcgat
     4561 aaagcggttc atacgctgcg catcggcttg cgcgtcttca cccggaaagg atttccaagc
     4621 cgggtagaat tgcaatgtaa ccgtattgtc cgcctcgcgg acagggatgg cgggtatcac
     4681 ttttgcattt gcaagcgcgg caatgcggct caatccggta atcgttgccg tccgaatacc
     4741 gaagaaatcc acaaaaaccg aatcgttgcg tccgaaatcc tgatcgggca gatacagaaa
     4801 cggcgcgctg cttttgcgga actgtttgac gagggcgcgc agcccttcgg tgcgcccgat
     4861 aaggaagacg ttgtcgtagc ggttgcggcc tttcaaaatc tgtgcgtcca atatcttgtt
     4921 tttttggtgg gaatacatac taattagcgg gacatcctga ttaagcgcgt acaccgccat
     4981 ctcgaacgcg gtaaagtgcg gatacaggat gatgactttt tcccccgccg ccagcgcgtc
     5041 gtccaaataa tgcttattgc ggtagcgcac cagcgatttc aaacgcccgg cgggcgcgta
     5101 ccaatataag ccgtattcga gcatcagttt cgccatatgt ttgaaatgct gtttcaacac
     5161 ggttttacgc ttttcctcac tccattcggg aaaacatttc gccaaattga tttcgccgat
     5221 acggcggcgg ggtttgacca aaaggtaggc aagcaaaccc gtcagatcgg caagtttgtg
     5281 cagcagcgca aacggcagaa actgcaaaac atacagtaca aaaaatataa atttcatctc
     5341 gatacacatt ttcttttcag acggcaaaat acaaatgccg tctgaaacta ttgaaacctg
     5401 ccgcgcttga cctgcatccc cgaaggattg agtttggcgg caagcccgtg gttgcgtaag
     5461 gcgtgggtca gcgcgacggc aagaccgtcc gccgcatccg cctgcggcgt tcccgaaagc
     5521 cccagcatct gcaccaccat atgctgcacc tgttctttcg ccgccttgcc tttgccgact
     5581 accgcctgtt tgacctgcaa ggccgtgtat tccgaaacgg gcagcttatg gctgaccaat
     5641 gccgccaatg ccgcgcccct agcctgaccc agcatcagcg tcgatgcagg attgacgttg
     5701 acgaacacct gttccactgc cgcctgttga ggcttgtaaa cggcaacgac ttcgccgata
     5761 tgccgcacaa tcacggcaat cctgtctgcc agagacgcat cggcaggcgt tttgatgcag
     5821 ccggaggcaa cataaaaatg atcccgcccc ctgacatcga tgatgccgaa acccgttacg
     5881 cggctgcccg ggtcaatgcc caagatacgg acggttgccg acatattcac aacaaaccgt
     5941 gttgaatcag ttttttacgc aaggtattgc ggttcagccc cagcatcacg gaggctttgg
     6001 actggttgcc gccgcattgc tccatcacgc acaccagcag cggtttttcc acctgatgca
     6061 agaccatatc gtacacgccg caaggttcgg taccgttcag gtctttgaaa tattgttcca
     6121 aattttgtct gatacattgg gaaatatcgg gaagggtatg gggcataatc gcgctttcaa
     6181 aaggataatc gggtgttcag acggcatttt ggcggtaggc gcacgcccaa cggtcggttt
     6241 ttccggcaag tctttcaaga taacctgcaa gcatgtcgta ttgcgccgcc gcactgtcca
     6301 agcggttgat ttcacgacgt gtctgttcgc cgtcgggcat ttcgtcgatg taccagccta
     6361 tgtgtttgcg tgcaatgcgc acaccggcgg tctcaccata aaacgcgtgc atggcgcgga
     6421 cgtggttcaa aatggcggca ctgcattctg ccaaactcaa ggcaggcggt aaaacaccgt
     6481 gttcggcgta atgtttcaaa tcgcggaaga accacggtct gccttgcgcg ccgcgcccta
     6541 tcatgatgcc gtctgcggcg gtttgtttaa ggacggcttg ggctttttgc ggcgaagtaa
     6601 tgtcgccgtt gacccaaacc gggatgttca gacggcattt ggtttcggca atcaggtcgt
     6661 aagccgcttc acctttgtac atttgcgtgc gagtgcgtcc gtggacggca agggcggcaa
     6721 tgccgcaatc ttcggcgatg cgggcgacgg caggcaggtt tttgtggtcg tcgtgccagc
     6781 ccaaacgggt tttgagggta acgggtacgt ccaccgcacg gacgacggct tcgagaatgg
     6841 cggcaaccag cggctcgttc tgcatcagcg cgctgccggc ttgggcattg cacactttct
     6901 tggcgggaca gcccatgttg atgtcgataa cctgcgcccc aaggctgacg ttgtaacgcg
     6961 cggcatccgc catctgctgc ggatcgctgc cggcaatctg cacggcaacg atgccgcctt
     7021 catcggcaaa atcgctgcgg tgcagggtct ttttggtatt tctaagtgtc gggtcgctgg
     7081 tcagcatttc gcacaccgcc caacccgcgc cgaaatcgcg gcagaggcgg cggaagggtt
     7141 tgtcggtaat gcccgccatc ggtgcaagtg cgatggggtt gtcgataaaa tagccgccga
     7201 tgtgcataat ggatccgcgt ttcaaaaaag tgcgccattg tacatttttt aagcaggatt
     7261 tccaatctcc ggacgcgccc gcgattgggg cggacaccgt tttatggcat aatccgcaca
     7321 cagattccct gccccgccac tcacaggcgg
//
LOCUS       DAXHMX010000104         7336 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHMX010000104 DAXHMX010000000
VERSION     DAXHMX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.104"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(174..986)
                     /gene="murI"
                     /locus_tag="ACFM4S_001571"
     CDS             complement(174..986)
                     /gene="murI"
                     /locus_tag="ACFM4S_001571"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6051219.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1188..1649
                     /gene="tsaE"
                     /locus_tag="ACFM4S_001572"
     CDS             1188..1649
                     /gene="tsaE"
                     /locus_tag="ACFM4S_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6051220.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYLLERFTLHHFDLYRFTTPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            1646..2896
                     /locus_tag="ACFM4S_001573"
     CDS             1646..2896
                     /locus_tag="ACFM4S_001573"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6051221.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRVPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYLNTSVLKRG"
     gene            complement(2954..4096)
                     /locus_tag="ACFM4S_001574"
     CDS             complement(2954..4096)
                     /locus_tag="ACFM4S_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6051222.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERNIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKYYAG
                     WLAAMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVEGSNR"
     gene            complement(4145..4567)
                     /locus_tag="ACFM4S_001575"
     CDS             complement(4145..4567)
                     /locus_tag="ACFM4S_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051223.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(4560..5369)
                     /locus_tag="ACFM4S_001576"
     CDS             complement(4560..5369)
                     /locus_tag="ACFM4S_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6051224.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEYAGSVWMMVQTREQWRQAQEKGADVVVWRVGNAAQ
                     AAAVDEVLKHGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(5451..5828)
                     /gene="acpS"
                     /locus_tag="ACFM4S_001577"
     CDS             complement(5451..5828)
                     /gene="acpS"
                     /locus_tag="ACFM4S_001577"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6051225.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(6028..6795)
                     /locus_tag="ACFM4S_001578"
     CDS             complement(6028..6795)
                     /locus_tag="ACFM4S_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6051226.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(6805..>7336)
                     /locus_tag="ACFM4S_001579"
     CDS             complement(6805..>7336)
                     /locus_tag="ACFM4S_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC6051227.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1770 a         2007 c         1814 g         1745 t
ORIGIN      
        1 gtttcgagat tacagtgtat cgagaaggat gggaaacggt gggagttgtg taaaaaatgc
       61 cgtctgaagc ctttcagacg gcatttgcgg agacggggcg tttaatacgg cagggcattg
      121 caggtgcgta tgcggatggc gcggacgggg acggtttccg tccgtacctg ccgctacccc
      181 aaagacacca tttcaatctg ctccatcgtc ctgcccagaa aacgctcgcc gatggttctg
      241 aatttcaaag gaatatcgct gacgtaaaaa cggtagtcgg gattgttgtt gtcggtattg
      301 agcaatcctt cctgagcaag gacgcgtgcg gtttcttcgg cagtcgtaat tgcagaatca
      361 accaacgcga cattgtgcgc ctccctgccg attaagggct tgagcaaggg aaagtgcgtg
      421 cagcccaaca ccagcgtatc gatgccgtct gcaagcaatg gtttgaggta ttcgcatacg
      481 gtcaggcggg taacttcatg ttccaaccaa ccttcctcca ccaaagggac gagcagcggt
      541 gcagcctgcg tgcggacgag cgtatcaggg ttgttcctat ggatggcgcg cgcataagca
      601 ttgctgttaa ctgtggtatt ggtggcgata atgccgattt tattgttgcg cgtcgttgcc
      661 agcgcggctt tcgcgccggc ggaaatcacg tccaacacag gcatattgcc cgtcttttga
      721 cggattttct gccccgccac cgccgcaatc gtgttgcacg cgataaccat cgccttgaca
      781 tcgtgttcca gcagaaaatc gacaatctgc atcgagaaat tttcgatggt cgccttagat
      841 ttcgtcccgt aaggcacgcg cgccgtgtcg ccgaaataaa tgatgttctc catcggcagc
      901 cgctccatca gcgcgcgcac attggtcaaa ccgccgattc ccgagtcaaa aacgccgatg
      961 ggtcgctgcc tgccgatatt ttccatcctt tttccaatct gtcctaaaca tacaatccgc
     1021 gtattgtaca cgcgccgctt tttcttgaca gccgttgccg tctgaaagca gaacgcggat
     1081 ttggctgtta taatgctcgg acaagaagca tccgcccctc gggtgcaaag tttgcaaaac
     1141 ctgcaaaact gcccataata ccctagacct tacaacaaac cgtttccatg tctgatttac
     1201 cctctatctc ccgattcctt gccgacgaag ccgccacact cgatttgggt gcggcgtggt
     1261 cttcccgttt aaacgcgccg ctggtcattt atctcgaggg ggatttgggt gcgggcaaaa
     1321 ccacgctgac acgcggcatc ctgcgcggat tgggtcatca gggcgcagtc aaaagcccga
     1381 cttacgccat cgtcgaatct tatctgctgg aacgcttcac cttgcaccat ttcgacctct
     1441 accgtttcac cacacccgaa gaatgggaag atgccggatt ggacgaactg tttgccgcaa
     1501 acagcgtctg cctaatcgaa tggccgcaac agggcgggga atttacgccg cctgccgaca
     1561 tcaccgcaac attgacacac gacggcgacg gcagaaaatg cctgctgacc gcccataccg
     1621 aacgaggacg cgaaagcctg ccgctatgac caaactgaca cgaagacaaa tcatccgccg
     1681 caccgccggc acactgttcg ccctaagccc catcgcatcc gccgttgcca aaacggtacg
     1741 cgtaccacaa ttcaccgccg cacggatatg gccgtcccac acctacaccc gcctgacgct
     1801 ggaaagcacc gccgcgctca aataccagca cttcgcgctc gacaatccgg gcaggctggt
     1861 cgtcgatata caaaacgcga acatcaatac cgtattgcac ggactttccc aaaaagttat
     1921 ggcggacgac ccctttatcc gcagcatacg cgccggtcag aacacgcccg acaccgtccg
     1981 cctcgtcatc gatttgaaac agccttccta cgcgcaagtt ttttccctcc cacccgtcgg
     2041 cggctttaaa aaccgcctcg tcgtcgatct ctatccgcaa gggatggatc ccgacgatcc
     2101 gatgatggcg ctgcttaacg gcagcctgaa caaaacccgt cgcggctccc ctgaagccga
     2161 cctcgctcaa aacactcccg cacaaaccga acgcggcaaa aacgggcgca gacccgtcat
     2221 tatgatcgac cccggacacg gcggcgaaga ccccggcgcc gtcagcccgg gcggtttgca
     2281 ggaaaaacac gtcgtcctct ccatcgcccg cgaaaccaaa aaacaattgg aagccttggg
     2341 ttacaacgtc tttatgacgc gcaacgaaga cgtgttcatc ccattgggcg tgcgtgtcgc
     2401 caaagggcgc gcacggcggg cggacgtatt cgtctccatc cacgccgacg ctttcaccag
     2461 cccctccgcg cgcggaacgg gggtttatat gctgaacacc aaaggcgcga ccagctctgc
     2521 cgccaaattc ttggaacaaa cccaaaataa tgccgacgcg gtcggcggcg taccgaccag
     2581 cggcaaccgc aatgtcgata ccgccctgct cgacatgacc caaaccgcca cgctgcgcga
     2641 cagccgcaaa ctcggcaaac tggtgcttga agaattgggc aggctcaacc atctgcacaa
     2701 aggcagggtg gacgaagcca atttcgccgt tttgcgcgca cccgatatgc cgtctatcct
     2761 ggtcgaaacc gccttcctgt ccaatcctgc cgaagagaag ctgctgggca gcgaatcctt
     2821 ccgccggcag tgcgcccaat ccatcgcctc gggtgtccaa cgctacctca atacatccgt
     2881 attgaagcgg ggttgattcc gaaaaataaa tgccgtctga acatttttca gacggcattt
     2941 ttgtacactt tcctcaacgg ttcgatcctt ccaccatatc aatcaggaac aggcggcaat
     3001 cgaggttgcc gttataaagc ggaattttgc gttttggcga caggcgcatg aatttgggca
     3061 tttcccgatc tccggtaaac attgccgcca accagcctgc gtaatatttc ttcaaccacg
     3121 tccccaactg cggataaagc gcctgcaagg cgcggacttc ctcaaggcgc acgccgtagg
     3181 gcggattgga caccataatg ccgcccacgc cgttcggacg gacggattgc gcgtcggcaa
     3241 cgctgaagga aacgatgtcg tccaccccgg cactgcgtgc gttggccaat gccgtctgaa
     3301 tgatgcggcg gtcgctgtcg ctgcccgaaa tcggtgcaaa agccggttta acctgcgctt
     3361 cggcacggcg gcgcaaatcc gaccacagcg ttttatcgaa attttgcagt ttttcaaaac
     3421 cgaaacggcg catcataccc ggcgcgcggc gggcggcaat ccaagcggct tcgatagcaa
     3481 tcgtgccgct gccgcaaaac gggtcttgaa acggctgcgt gccgtcgtag cctgccgaga
     3541 gcagcaatcc ggcggcaagg ttttcgcgca gcggggcttc gccggtatcg agacggtagc
     3601 cgcgtttgaa cagggcttcg cccgaagtgt cgatgaagat ttcgacattg cgttcgtcca
     3661 aaaaggcgtg gatgcgaaca tcgggcgcgg ctttgtccac gctcggacgt gcgtcgtaaa
     3721 tgtcgcggaa agcgtcgcag acggcatctt tgacggtcag tccgacaaat tggatgctct
     3781 taacgttggc acgctttgcc tcgactttga ctttgaacgt ctgctgtaaa gtaaaccaat
     3841 taaaccagtt gatatttttg gcgagtttgt agatgttgcg ctcattgcgg tatgtccctt
     3901 tggtcaggcg cagcaggata cggctggcag tacgcgaatg caggttggcg gcgtaaacct
     3961 gttccaatcc gccccggcag gaaacgccgc cgtcaaacac ttgtacatcg gtacagccga
     4021 ggctttcgag ttcttgagat aaaacggtct ccaagccgcg cgggcaggtg gcgaaaagtg
     4081 tatataaagt attcataacg tgtcctttcc ggacggatgt tccggcaatg gcggattgaa
     4141 aggtttattc ggcggtaaat gccgtctgaa atgtccgact gccgattgta atgtctgccg
     4201 taaaatcgcg cctgccttcg acacagatgg gcaggcggat gcgtactgcc tgccaagtcc
     4261 ccgacggttg ccgctcgaac atatagcggt tgaaacccat atccatattt ttcatactga
     4321 agctgatgct gacctgttcc gtgccggcgg gcgcgtgttc gatataaata tcaaacggtt
     4381 ttttggttga aacggcggcg gcgcggacgc ggcttccgtc cggcagcgtg caaccctcgg
     4441 tcaaatcgca ttgcgccgcc acagcttgcg gctgctgcgc ctgccaccat tgcaacaaaa
     4501 cgagcttgac ggcggcaaag gcaatcagca gcagtgcggc aagcagtaat ttacggtttt
     4561 tattcatgat tcttccctat tgtttcatcc cttaccacca cgtgcgcccc caatccgagc
     4621 catagttttc cataacgtgc aaccgtctgt ccgtttgctg caagtacgag cggcacggat
     4681 acgccgtgct tcaacacttc gtctaccgct gcggcttgtg cggcattacc gactcgccag
     4741 acgaccacat ccgcgccctt ttcctgcgcc tgccgccatt gttcgcgggt ctgcaccatc
     4801 atccacacgc tgccggcgta ttcgggtctg tcctgccatt gcgccgcctc catcaaaacg
     4861 ttcgcaccgc ttccctctgc cgaacccaaa ggcaggacgc ggtacgcgcc catactgttt
     4921 tctccgtgca aacccgtttt caggctgccg tacaaacggc gcgggacgga cagcgaacgc
     4981 aaaagcgcgc cgttggcggg cagcatgggg gcaacggtaa aatcacccgc cttctgccaa
     5041 gaccattcct gcccttcgcg ggattgcggt tcgcccgtcc attggtcggg gttgacccat
     5101 aggaatttca ggcagacgcg ggcgtgttcg taggaatgga ttttggtcaa ccaaggcgtg
     5161 gcggcgagga tgcggatgcc gagttcttct tcaaactcgc gttgcagggc ttggaagtcg
     5221 gtttcgcccg cttcgacctt gccgccggca aattcccaat atccggcata gggtttgcct
     5281 tcggggcgcg agctgagcag gtagttgccg tctgaatcga gcaggatgcc ggcaacgacg
     5341 cggataaggg ggcgggtgtc ttgaatcatg gcgggtacgc ggttggtcgg tcggagtgca
     5401 aatgagggat gggcaatgcc gtcaacgggt tttccgcatt tcagacggca ttatttttcg
     5461 gcaacgacaa acgccaatac ggtgtcttct tcgtcgctca tgctgaggct gacgcggctg
     5521 atgccttgtt cctccagcca tttggacagg gcggggccgt agaaaaattc gggcttgccc
     5581 aatgcgtcat gcccgatgcc gatgttgcgg aaggaaaccg cgccgcgtat gcccgtgccg
     5641 acggctttgg cgaaggcttc tttggcggca aagcgtttgg cgaggtagtt gacgggtttg
     5701 cccgcttgcg gaaattcaag cagctcttcc ggagtgagga tgcgcccggc aaacgcctgt
     5761 ccgaattttt tgtttaagcg gatgatgcgc ttgagggaaa caatgtctgt gccgatgccg
     5821 taaatcatat ttgcgctcct tcgcccttgg ttgcgggtag tgatgaatgt gatgatgaat
     5881 gccgtcaatt tcttggggcg ttaaaatggc gtgaatatcc acttttgggg ttttgtcggt
     5941 aacaattttc actgtaacct tttgcatttg aaattctaaa acatcgtgcc aattatttaa
     6001 taaggagtta aggcatttaa taatgaatta aagtttgatt aaacccgaat gcccgatttt
     6061 cagagccaat accaataaaa caataaaaat taacatttta aaaaaatttg ggctaatctt
     6121 agtccttaac cgaattccaa catacaatcc aataacagac aatacggaca gtaaaaatat
     6181 taaaccgtat tcactcttat ttaataacca atactggtct cttagcatat atatttgaac
     6241 aattttcgcc aaaagatagc atagattgct tgattttacg atacgatttt tattttctgt
     6301 ttcgctaagc aaaaatatta acaatatggg agacatggca ttggttgaac cgccgatgat
     6361 gcctgccaaa aacccaaaaa gaaccatatt cttattattg gcaactactt gaatattttt
     6421 tgcttttgca catacattta aaataccatt gacagaataa tacaatgtaa tgattgccat
     6481 cagtaaaagc agccaagaca ctggaagtat caaaagcaac ttcaccccca aaatgctgcc
     6541 aacgacgctg ccgatagcaa gcaatttata ggtttttaaa taataaacaa tctcttgcca
     6601 aaaacccttt ttgttattgc tgcatagaac caacaagctc attaacaggc ttggtaatgc
     6661 caccaaggca acaaccttag acaatggcat gataaaagcc aatgcggttg taccgagcat
     6721 cggaaatccc atgcctgtaa ttccgtgcag tattgcggca gcaacaaaaa cgatagattg
     6781 cattatttct tgcataaccg tcctttatac agtaattctt gctctaatat aatgcctgtt
     6841 tttatccgta taatcagttc tggcatgcaa gccattgaca ttgtcaataa taatcaaacc
     6901 atcatcttgt gcagaaaatt cttgaatatc actcgcattt tttataacat ttacaagata
     6961 atcaagtgca agtaccattt cctttgatac tttatttcta ttttcttcaa tacctttata
     7021 gatacaatca cttctaaatc taatcatttg agtattgacc gataagatat taccccatct
     7081 cacaccttgt tttttatcaa atgatgctgg tgttttaaat ggatataaat tgcccgttaa
     7141 tgtttttaag tgttttttac cggtttctgt tttagataac tgattgacaa tatctgatga
     7201 acttaaaaat agggaattac ctccatcatt ggctgatttt actacataca taaccaaata
     7261 actttccggg ttttcactaa aagatgaatc actatgaagt ggacattctc caacatcttc
     7321 cgagaaagtt atatca
//
LOCUS       DAXHMX010000105         7304 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHMX010000105 DAXHMX010000000
VERSION     DAXHMX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.105"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            1..99
                     /locus_tag="ACFM4S_001580"
                     /pseudo
     CDS             1..99
                     /locus_tag="ACFM4S_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(120..1034)
                     /locus_tag="ACFM4S_001581"
     CDS             complement(120..1034)
                     /locus_tag="ACFM4S_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6051228.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1302..2771
                     /locus_tag="ACFM4S_001582"
     CDS             1302..2771
                     /locus_tag="ACFM4S_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6051229.1"
                     /translation="MDKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3023..3394
                     /locus_tag="ACFM4S_001583"
     CDS             3023..3394
                     /locus_tag="ACFM4S_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051230.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLASSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3467..4876
                     /gene="thrC"
                     /locus_tag="ACFM4S_001584"
     CDS             3467..4876
                     /gene="thrC"
                     /locus_tag="ACFM4S_001584"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6051231.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGSAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPA
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGEMVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4922..5716
                     /locus_tag="ACFM4S_001585"
     CDS             4922..5716
                     /locus_tag="ACFM4S_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051232.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDAQIRQEAEAGKSDAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5904..6680
                     /locus_tag="ACFM4S_001586"
     CDS             5904..6680
                     /locus_tag="ACFM4S_001586"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6051233.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6756..6862
                     /locus_tag="ACFM4S_001587"
                     /pseudo
     CDS             6756..6862
                     /locus_tag="ACFM4S_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6933..7076)
                     /locus_tag="ACFM4S_001588"
     CDS             complement(6933..7076)
                     /locus_tag="ACFM4S_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051234.1"
                     /translation="MENNKVKSDYAAKQESLGKVDENKNETMKIPPQVGTHMLGFSFI
                     FKC"
BASE COUNT         1789 a         1804 c         1910 g         1801 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agtttttcgg atgatgatta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtga cggcttcctt tcggtggccc gaacgcagcc catatcaaaa agaaaagtca
     1261 tgcgcccctt ttgcgaggcg cgatatataa ggaggaagat tatggacaaa cataatggga
     1321 cttatcggga tttgcaccgt ccggcttcgg aatttgcgac gcgggacgaa tatttggaac
     1381 atgaattgca gattatgcaa ccaaaacgct ggcggcccaa cctgcccttt cgcgattacc
     1441 gcttcgagtg ggaggatttg attcctgcga tggcgggaac gattggaaaa gtggtgatgg
     1501 tgggggcggt ggcggcggcg tttgccgcac ctttggggct gcctgacagc tttgtactgg
     1561 aaaatgtgcg ctatgagctt ttaatcgccg ccgcgtttat cttattggta tcgggctttt
     1621 ttctgcccgg cgccaacctg cccggtacgc acgggccgct gattccgatg attcccatcg
     1681 ttgtgtcggc aggcgggcat cctttggcgt tcggcatttc gattgcggtt ttaggtctgc
     1741 tgatggcttt atttcgcggc ggcagtatta tggcgaagct gacaagcaac ggcgtatgcg
     1801 gcggattatt actctatttg ggctttatcg gcacgacggg gcaggtaaaa aaattgtttt
     1861 cgtgggcagg cggttttaat atgccctaca tcgcttttac cgtcattatt gtaacgattg
     1921 tgatgtacgc tttgttggag cattggaaaa aacgctggtt agccgtgcct ttgggatgct
     1981 tgattgccgg tgtggtggca tttgcattgg gtgcgccgtt tgagtttcac accgcccccg
     2041 gcctgcctcc aatgagtcct gcttattggt ggggtgaaaa cagcggctgg catctggggt
     2101 tgccgacggc agaaagtttt ttggttgtct ttccatttgc ggtattggct gttgcaatgt
     2161 ggtcgcccga ttttttagga catcaagtgt tccaaaaaat cagctatccg gaaaaaaccg
     2221 ataaggtatt gatgaatata gacgacacca tgacaagttg ttctgtccgt caagcagtgg
     2281 gttctatttt agggggtgca aattttacct cttcttgggg aacttatatc gtaccggcat
     2341 cgattgccaa acgccccatt ccgggcggtg cggttttaac ggcggtttta tgtattatcg
     2401 ccgggttatg gggctatccg atggacttgg cgatttggca gccggtattg agcgtagcct
     2461 tggtcgtagg cgtatactta ccgcttttgg aagcgggcat ggaaatgacg cgcaaaggca
     2521 aaaccaccca atccgccgcc atcgtggtgt tctcttccgc cttggtcaat ccggttttcg
     2581 gctgggcgtt gacgatgctg ttggataatt tgggcttaat cggctgcaaa gagcgcagtg
     2641 cgcaattagg tttcgccgga cgcgtgttga tacccgcagt aggtttcttg atcttgtgtg
     2701 tggcgatggg tgcggtcggg atgctgcccg gtatcccgcc gtttttggaa cacttcaaat
     2761 ctttgggcta ggctgaaatc ggaaatgccg tctgaaccgc tttcagacgg catttttgca
     2821 aacaggcaaa atgacggcgg cgggattttt tattttcccg attgaagtat aatgttgccg
     2881 ggcttcaacc ggatattcaa aacggtttgt tccaacactc ggaacggcgc ataaaacgcc
     2941 gcccttcgcg ttatcccgaa cggggcggct aatcagattc tatcgccata aaaggcgggg
     3001 tttcaaccga aaaggaattg agatgaataa aaccttgtct attttgccgg tggcaatctt
     3061 actcggcggc tgcgccgccg gaggcggtaa cacattcggc agcttagacg gtggcacagg
     3121 catgggcggc agcatcgtca aaatggcggt tgggagccaa tgccgtgcgg aattggacaa
     3181 acgcagcgaa tggcgtttga ccgcgctggc gatgagtgcc gaaaaacagg cggagtggga
     3241 aaacaagatt tgcgcttgcg tcgcccaaga agcacccgaa cggatgaccg gcaacgatgt
     3301 gatgcagatg ctggcttcgt ccacgcgcaa tcaggcactt gccgccctga ccgccaaaac
     3361 ggtttccgcc tgcttcaaac acctgtaccg ctaaccttct tcagacggca tgaggcagat
     3421 gccgtctgaa gccgttttcc tgcaatttcg acagacgaga aaaatcatga aatacatcag
     3481 cacgcgcggc gaaaccgcac acaaaccgtt cagcgaagtt ttattgatgg ggcttgcccc
     3541 cgacggcggt ctgatgctgc cggaacatta tccgcaaatc gggcgcgaaa ccttggacaa
     3601 atggcgcggt tcggcttatc ccgaattggc gtttgaaatt atgcgcctgt tcgttacgga
     3661 tattccggag gacgatttgc gcgacattct gaaccgtact tacacggaag cggcgttcgg
     3721 tactaaggaa atcacccccg tccgcacgct ttcagacggc atcaaaatcc aagccttgtc
     3781 caacggcccg acgctggcgt tcaaagatat ggcaatgcag tttttgggca atgcgtttga
     3841 atatgtttta aacaaagaag gcaaaaaact caatatcttg ggcgcgacca gcggcgatac
     3901 gggttcggct gcggaatatg ccttgcgcgg caaaaagggc gtgaacgtat ttatgttgtc
     3961 gcccgacggt aaaatgagcg cgttccaacg cgcgcaaatg tacagcctgc aagacgagaa
     4021 tatccacaat atcgccgtga agggaatgtt tgacgactgt caggatattg tgaaggcggt
     4081 gcagaacgat gccgcgttca aggaaaaata ccatatcggt acggtcaact cgatcaactg
     4141 gggacgcatc gtcgcgcaag tggtttatta ctttgcaggc tatttcaacg ccacttcaag
     4201 caatgacgaa accgtcagct tctgcgtacc gagcggcaac ttcggcaatg tttgcgcggg
     4261 acacattgcc aaacaaatgg gcctgcctat ccgccgcctg attgtcgcga ccaatgaaaa
     4321 cgatgtgttg gacgagtttt tcaaaaccgg tgcataccgc ccgcgcaaca gcgcgcatac
     4381 ttatgtaacc tccagcccgt ctatggacat ttccaaagcg tccaacttcg agcgtttcgt
     4441 gttcgacctg atggatcgcg atcctgcgga aatcaatacg ctgtgggcgg aagtggctgc
     4501 aggcaaaggc tttgacctgc gatttgcctt ggacaaagtc ggtggcaaat acggttttac
     4561 ctctggcaaa tccacccacg ccgaccgcct cgccaccatc aagcaggttt acgagcaaga
     4621 ccaagaactc atcgacccgc acactgccga cggcgtaaaa gtcgcccgcg aagtgcgcga
     4681 agaaggtgaa atggtggttt gtttggaaac cgcgttggcg gcgaaattcg atgcgaccat
     4741 acgcgaagcc gtcggcgatg ccgccattcc gcgccctgcc gcgctggaag gtttggaaaa
     4801 cctgccgcag cgcgtgcaaa ccgtgccgaa cagtgcggat gcggtaaaag gcatcatcga
     4861 acaaaccctt gcctgatatt ccgtacaggg gcattccgtc cgaacttaag attgaaagaa
     4921 aatgacctta cctatgcagg aaacccgttt ttccatactg ctagacgaat tggcggcaaa
     4981 acaggaggca gccatcgccc cccacctgct tgccgacgga accaaggtat ggatacgcaa
     5041 agcaggcagg cacaatgcac gatggcgtta cgcgctgctc ggtatggttg cccgatattt
     5101 gaaactgggg gtgttgaagc cggttcccag cctcggcggc gagcctgcca ttgcaaccga
     5161 atcaaaacgc ctgtacgaat tgcgctcggc ggggatagcc gttcccgaat tgctcgccgt
     5221 caggaaaaat gcccttatgt tcggcaattt ggaaggcatc ccgctcgatg cgcaaatccg
     5281 ccaagaagcc gaggctggaa agtcggacgc ttggcttgcc ggtttggaag ccattgcacg
     5341 cgtgcataaa aaacagcagt tcctcagtca ggcgtttgca cgaaatatga tgtgggacgg
     5401 aaaaaacatc agctttttgg attttgaaga cgatccttcc gaagtcctga ccatagcgca
     5461 atgccaagcc cgcgattggc tctgctacat tcattctacc gcgctaatat tgaaaaacgg
     5521 cggactgctg gaagctgctg cggaaaaatg gggcggcgta ttgtcggatc agcctgccga
     5581 aatacaaaag ctgattgccg ccaccgtcaa accgatcctc ccgatacgca ggctggaaca
     5641 cccccgctgg ggacgggacg cgctcaggct ggcagcctcg atttccctga tttcccttgc
     5701 cgatatgccg ccataagccg ccgcccgccc cacgatgccg tctgaagccc ttcagacggc
     5761 atataaccaa cgcgcatcaa gcattgtcgc aaccatccgt ttcgtttaga attttgactt
     5821 tatcccgaat tgggcagata ttctcattct gacacacgtc ccaaataaac actccccatt
     5881 tcttttaccg acaggaaacc caaatggcag cattcaatac ccaaaaagta ttgtccgtac
     5941 accactggac agacgcatat tttaccttta cctgcacccg cgacgaatcg ttgcgctttg
     6001 aaaacggcca attcgttatg gtcgggctga tggtggacgg caagccactg atgcgcgcat
     6061 acagcgtcgc ctccgccaac tgggaagaac acctcgaatt tttcagcatt aaagtccaag
     6121 acggcccgct gaccagccgc ctgcaacacc tcaaagtcgg cgacgacgtg ttaatcagca
     6181 aaaaaccgac cggaaccttg gttgccggcg acctgaatcc gggcaaacac ctttacctgt
     6241 tgagcaccgg taccggcatc gcccctttct tgagcatcac caaagatccc gaaatttacg
     6301 aacagtttga aaaaatcatc ctcgtacacg gcgtgcgcta caaaaaagat ttggcgtact
     6361 acgaccgctt taccaaagaa ttgcccgaac acgaatacct cggtgacttg gttaaagaaa
     6421 aactgattta ctacccgatt gtttcgcgcg aagaattcga acaccacggc cgcctgaccg
     6481 acctgatggt gagcggcaaa ctgtttgaag acatcggcct gcccaaaatt aatcctcaag
     6541 acgaccgcgc catgctgtgc ggcagccccg cgatgctgaa agacacctgc aaagttttgg
     6601 acgatttcgg actgaccgtc tctccgaaaa caggcgtacg cggcgattac ctgattgagc
     6661 gcgcatttgt ggaccaataa tctgcttgaa aatgccgtct gaacggcatc tgcgataaaa
     6721 aaccccgaat cctgccaatc aggattcggg gtttttatag tgaattaaat ttaaattagg
     6781 acaaggcggc gagccgcaga cagtacaaat agtacggcaa ggcgagccaa cactgtaccg
     6841 gtttaaattt aattcactat atagtgggta aaaattagaa atgcacattt ccgtcattca
     6901 cgcgcaagtg ggaatccaga cctatccgtt aattagcatt tgaaaataaa agaaaaacca
     6961 agcatatggg ttcccacttg cgggggaatc ttcatcgtct catttttgtt ttcatcaact
     7021 tttcctaaac tttcctgttt ggctgcataa tcagacttga ctttgttatt ttccatattg
     7081 agcttctctt aatttgaaaa aattaacata aacatttaaa aataaaacaa aattatataa
     7141 taaaaaaaat aatgagaata taaaggataa aaagagagtt aagttattta atcggacttg
     7201 ttctctataa atcataagac accaaataag ccataataaa tttagtaaat aaaaactgct
     7261 tatacccaac tttcttttct tggaacctat tattatttca actc
//
LOCUS       DAXHMX010000106         7224 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHMX010000106 DAXHMX010000000
VERSION     DAXHMX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.106"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(16..771)
                     /locus_tag="ACFM4S_001589"
     CDS             complement(16..771)
                     /locus_tag="ACFM4S_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC6051235.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPKWQKGKVSSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNADAQMFCSGASVGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(951..2126)
                     /gene="nirK"
                     /locus_tag="ACFM4S_001590"
     CDS             complement(951..2126)
                     /gene="nirK"
                     /locus_tag="ACFM4S_001590"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC6051236.1"
                     /translation="MKRQTLAALIASVFALAACGEQAAKPAETPAATASAEAPAASNS
                     QAAAETPSSELPVIDAIVTHAPEVPPPTDRDHPAKVRVKMETVEKTMKMDDGVEYHYW
                     TFDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTS
                     TFSFKALQAGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTK
                     GKKGAQGLQPFDMDKAIAEQPEYVVFNGHVGSIAGDNALKAKAGETIRMYVGNGGPNL
                     VSSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFR
                     AFNKGALGQLKVEGEENPEIMTQKLSDTAYAGNGAASAASAPAASAPAASASEKSVY"
     gene            2524..4779
                     /locus_tag="ACFM4S_001591"
     CDS             2524..4779
                     /locus_tag="ACFM4S_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC6051237.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELVAWLDLTAQE
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVVSETRVKAIESILPYYHGVY
                     SDDPKFQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNVQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4958..5491
                     /locus_tag="ACFM4S_001592"
     CDS             4958..5491
                     /locus_tag="ACFM4S_001592"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6051238.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5554..6738)
                     /locus_tag="ACFM4S_001593"
     CDS             complement(5554..6738)
                     /locus_tag="ACFM4S_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6051239.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6778..7182)
                     /locus_tag="ACFM4S_001594"
     CDS             complement(6778..7182)
                     /locus_tag="ACFM4S_001594"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6051240.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1691 a         1719 c         1957 g         1857 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacgtattt
       61 ggattgtagg ctggtgcgga tgccgtagcg gaggaatgcg gcatagttgg acgagtcgct
      121 cgacccgaca gatgcgccgc tgcaaaacat ttgcgcatcg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagaccg tgcatatcgt aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggccttta cgaccgccat ccgcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cagcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcaggct ctgcctgttt
      541 ggaactgacc ttgccctttt gccatttggg gtgactgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      721 ttgggtgccg gcgagagccg cacagagaaa gagtaaccgg gcaaacttca tattatgcct
      781 cctgacaggc ggttaacatt gatttttcag acggcatttt aaacgagaac cgcgaatgcc
      841 gtctgaagaa tcaagcttcc aaagtaaaaa ggttgatgat taaaaaacca ctgccgtgcg
      901 taatgaagta cggcggcagt ggtttgttcc gctaatgacg gatatccgat ttagtaaacg
      961 cttttttcgg atgcagaggc tgccggagcg gaagctgcgg gagcggaagc tgcggaagcc
     1021 gcaccgttgc cggcgtaagc ggtatcactc agtttttgag tcataatttc agggttttcc
     1081 tcaccctcta ctttcaattg acccaacgcg cctttgttga atgcgcggaa gatagagtgg
     1141 tcgaccaagg tgtagctgcc tggaatgtcg actttgaatt cgacaatggc ggaaccgccg
     1201 gcaggaacaa ttgtactttg tacgttttcg ttaatcagtt taccaccttc aacatatact
     1261 ttgtcgaaga tttcgccgat aacgtggaaa gaagatacca agttaggacc accgttacca
     1321 acgtacatac gaatagtttc accagcttta gctttcagag cgttatcgcc ggcgatagag
     1381 cctacgtgac cgttgaatac aacgtattca ggttgttcag caatcgcttt atccatatcg
     1441 aaaggttgca ggccttgagc acctttttta cctttagtat agaagtcacc ttgtacgatg
     1501 tagaactctt tgtccacttt aggcagacct tctttaggtt caaccaagat caaaccgtac
     1561 ataccgttgg cgatgtgcat acctacagga gcaacggcac agtggtagat gtacaaacca
     1621 gcttgcagcg ctttgaagct gaatgtggaa gtacggcccg gagcggtaaa ggttgcagcc
     1681 gcgccgccgc cctgaccggt agccgcgtgg aagtcgacgt tatggggaac ggtagaagaa
     1741 ggattgttgg agaattccac ttcaaccgta tcgccttcgc gtacgcggat catgcggccc
     1801 ggaacatcgc cgtcaaatgt ccagtaatgg tattccacgc cgtcgtccat tttcatggtt
     1861 ttttcaacgg tttccatttt aacgcgtact ttagccggat ggtcgcgatc agttggaggt
     1921 ggaacttccg gagcgtgagt tacgatagca tcgattacag gcagttctga agaaggagtt
     1981 tctgcagcag cttgtgagtt ggaagcggca ggagcttcag cagaagcggt agcagctgga
     2041 gtttcagcag gtttagcagc ttgctcgccg catgcggcca gtgcaaatac agaagcgata
     2101 agtgcggcta aggtttggcg tttcatgttt aaagtttcct tatagtaaga aaagttaaga
     2161 atcctgatag gatggtttga aattttgtat gattatgtag gcgcttgttt ggtcatattt
     2221 tgatgtaaat caaattattc agggaatatg aatcttttac tacctaaata ctcctttaga
     2281 agtataaaaa aatacatcga aaagtttaat tttgtgtgaa aatgttagcg aaaatttaac
     2341 acaaatacga taaaggaggg tttgtataag aaaatgggaa tcaatatcat ataagttaat
     2401 ggttttaaag gaatattttt gacaaaatat ttctttactt gttaaaccgt gatacaatct
     2461 ttaacattca tatttgatga attaataccc aaccactttt accaaaatta aggagtactt
     2521 aaaatgggac agtacaagaa gctttggtac ttgctgtttg ccgttctggc ggtatgcttt
     2581 accattcttg gctatatggg cagcgaggtt tacaaaaaag ccccgcctta ccccgaacag
     2641 gtcgtttccg catccggcaa ggtcttgatg acgaaagacg atattttggc aggtcagtct
     2701 gcatggcaga ctaccggcgg tatggaggtc ggttcgattc tgggccacgg cgcatatcag
     2761 gctccggact ggacggcgga ctggctgcat cgtgagttgg ttgcctggtt ggatctgact
     2821 gcgcaagaaa cttacggtaa aaaatttgac gaagtttctc ctgaagaaca agctgttctg
     2881 aaaacacgtt tggctgacga ataccgcaac caaagccgtg ttaaggaaga tggttctgtt
     2941 gttgtcagcg aaacccgcgt gaaagccatc gaaagcatcc tgccttacta ccacggtgtg
     3001 tacagcgacg atcctaagtt ccaaaccact cgcgaacact ttgcaatgaa aaacaataca
     3061 ttgccaagcc aagaagcgcg tgaaaaactg ttcaacttct tcttctggac ttcttggtct
     3121 gcttcgacca accgtcctga cgagaatttt acttacacca acaactggcc gcacgaacct
     3181 ttgatcaaca acgtaccgac cactgaaaac tatatgtggt cattcaccag cgttgttctt
     3241 ctgctgatgg gcatcggttt gctgatgtgg ggttattcct tcctgaccaa acacgaagaa
     3301 gtggaagtgc cgtctgaaga cccgatttcc aaaatccaac tgaccccttc gcaaaaagca
     3361 ttgggcaaat acgtcttcct gacggtcgcc ctgtttgtgg tacaagtatt gctgggcggc
     3421 ctgaccgcac actacaccgt cgaaggccag ggtttctacg gtatcgacga ggcgttgggc
     3481 tttgaaatgt ccgactggtt cccctacgcc ctgacccgta cttggcacat ccaatccgct
     3541 atcttctgga ttgcaaccgg ctttttgacg gcaggtttgt tcctcgcgcc gattgtcaac
     3601 ggcggcaaag atcccaagtt ccaacgtgcc ggtgtgaact tcctctatat cgccctcttc
     3661 atcgtggtcg gcggttctta cgcgggcaac ttcttcgcgt tgacgcacat ccttcccccc
     3721 gaatttaatt tctggttcgg acaccaaggt tacgaatacc tcgatttggg tcgtttctgg
     3781 caactcctgc tgatggtcgg actgctgttg tggctgttcc tgatgttgcg ctgcacggtt
     3841 tccgccttca aagaaaaagg cgtggacaaa aacctgctgg caatctttgt cgcttctatg
     3901 gtcggtgtgg gcgtgttcta cgcaccggga ctgttctatg gcgaaaaatc cccgattgcc
     3961 gtaatggaat actggcgttg gtgggtggtt cacctgtggg tggaaggctt ctttgaagta
     4021 tttgccactg ccgccttcgc atttgtcttc tacaatatgg gctttgtccg ccgcagtacc
     4081 gccactgcct ccactttggc cgctgccgcc atctttatgt tgggcggcgt tccgggtacg
     4141 ctgcaccacc tgtacttctc cggctctacc tccgcctcta tggcaatcgg cgcctgcttc
     4201 tccgctttag aagtcgtgcc gctggtgttg ctgggtcgtg aagcatacga gcattggtct
     4261 taccaacacc tgtccgactg ggcgaaacgc ctgcgttggc cgctgatgtg cttcgttgca
     4321 gtcgccttct ggaatatgat cggtgccggc gtattcggct tcctgatcaa tccgccgatt
     4381 tccctgttct atatccaagg tttgaacact tctgccgttc acgcgcacgc cgccttgttc
     4441 ggtgtgtacg gtttcttggc attgggcttc gtattgctgg ttgcccgtta tttgaaaccg
     4501 aacgtgcagt ttgacgacaa actgatgact tggggcttct ggctgctgaa cggcggtttg
     4561 gtgggtatga ttgccatcag cctgttgccg gttggcgtga ttcaggcata cgcctccatc
     4621 acgcacggtc tgtggtatgc gcgtagcgaa gagttcctgc aaatggaaat cttggatact
     4681 ctgcgttggg ttcgtactgc tgctgacttg atcttcatcg gcggtgcaat ctgtgtcgcc
     4741 atccaagcca ctaaaatcgt attcggccgc gacaaataag ttttgcggta tgaaatgagc
     4801 aactgcaccc gttttcgggt gcagtttttt attgcggatt gggacgggaa tggaaaagga
     4861 tgtggatttt ttattttaat cggctataca attgctgctt tcatgccgtc tgaagcacgt
     4921 ttcagatggc atcggtttac atcagataag gataaaaatg ggtatttacg attttcaaat
     4981 gaaagatgca gaaggcaatg cggttgattt gtcaggctat cgcggcaagg ttctgctgat
     5041 tgtcaacacg gcaacgcgtt gcggtctgac cccgcaatac gaggctttgc agaagctgta
     5101 tgcacaatat accgcagaag gcttggagat tttggatttt ccgtgcaacc agttccgcga
     5161 acaggctccc gaaagcagcg gagaaattgc ccaagtgtgt atgatgaagt ttggcacgaa
     5221 gtttaagatt ttcgacaaaa tcgaagtcaa tggagcaaat accgcgccct tgtatgccta
     5281 cctgaaatcc gtcaaaccgc aagataaagg caatcatctg tttaaagatt tcgtgttgaa
     5341 gctcgcagct ttgggagaga agcgcgacga aggcgacatc aaatggaact ttaccaaatt
     5401 cctcgtcaac cgcgacggcg aagtggtcga gcgttttgcg cctagtgtta cgcccgaaga
     5461 aatcgaagcc gatattcggg cattgctgta atctgccgcc gtcttgggaa atgccgtctg
     5521 aaggcttcag acggcatttg tccgtttgcg ggtttattcc gaaatatcgg caacaccgct
     5581 ttcggggaag atgggcaaat ccggcggtgg gagcagggta tccaaagtca gggcggacgc
     5641 atcgagtgtg gggtagcctt caaacgcgac cgaataaccg ctgtttccgt tcatacggat
     5701 tcgggcgtgc gcgcccagag ggaaagtgat tttgtcggca atgtgcccga agggaaagcc
     5761 cgtcagcacg gggattttcg ccgtgcgcga aacatggttg gcaacggcgg aaaaatcata
     5821 agacggatca tagacatctc gaattttttc catacggaaa ttgccgaaca cgatggcgcg
     5881 ctgtttcttc aaaatacccg aaagatacag cgtattgagc atacgttcga tgcggtaggg
     5941 ctgttcgccg acatcttcga ggaacaaaat gccgccgtcg atgtcgggca tataaggcgt
     6001 gccggcgagc gaggcgagga cgcttaagtt gccgccccac aatatgcctt cggtttcgac
     6061 atcggcgcgt tggatataag gaacatcaac ggtcaggcgg ttttgggttg cacccttgat
     6121 aaaggcatcc atcgtaaacg caccgggggc gggtttgcca aaatcgctat aagccatcgg
     6181 gccggcaaaa ctcatcatat tgccttttgc caacaatgcc agctggacgg cgcatacgtc
     6241 gctgaatccg aaaaacagcg tgccgtgttc gcgcatcctt gcgccgagcg aagcaaaatc
     6301 gatatgcggc agaatccgcg ccgcaccgta accgccgcgc aagcccatca gcactttagg
     6361 cgtggcgacg cggccggaag cgacctcttg gaaatcggcg acacgttgtg cgtccgtacc
     6421 agcaaaccgt tggaagcggc ggctgcccgc ctgttggttg gttacggtaa aaccggcatt
     6481 gtaaaggcgg gttaaggctg tgttgacgcg gttggtgtct tcggcaaagc ctgacgaagc
     6541 gacaacgcgc agaaggttgc cgtccgaact ttgacggcgt ggcgtttgga gaggcacggt
     6601 tcgggctttc acaacagaat gggaagaggg aaggggcgga acggatgtgg cggatgtgcc
     6661 gcaagcctga agcagtcccg cgccggcggc ggcggtgcag gttttcagaa aacgacggcg
     6721 ggaggtcggt tcggtcataa tttccctttc atacggtttc agacggcatc ggcgggctta
     6781 aagcagcttt ttaagctgtt ccgtccaatc ttgcggcagg gtcataccgt gttttcggac
     6841 ggggtttgcc caaatcggcg cagggaagga cgcatcgttt tcaaagcggg cgataatatg
     6901 ccaatgcagg tgcggcacga cattgcccaa gctggcgagg ttaatcttgg caggttgaaa
     6961 cacttggcgc atagcggctt cgactttgta caccatttcc atcaattcgc cgcgttccgc
     7021 tgccgaaagg tcggtcattt cggcaatatg cttacgccaa atgacgcggc agaatgcagg
     7081 cgaaccgctg tcgttatgga cggcgatgac gcgcagcttg ggggtttgca gcaaaatatc
     7141 ttcatcttgt gccgtgcaga tggggcaggg ttgggcggtc atttttcctt attcctgaaa
     7201 gtgtttaaaa aacggtagga taat
//
LOCUS       DAXHMX010000107         7198 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHMX010000107 DAXHMX010000000
VERSION     DAXHMX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.107"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="ACFM4S_001595"
     CDS             complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="ACFM4S_001595"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6051241.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1556..1711
                     /locus_tag="ACFM4S_001596"
     CDS             1556..1711
                     /locus_tag="ACFM4S_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051242.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1708..2139
                     /locus_tag="ACFM4S_001597"
     CDS             1708..2139
                     /locus_tag="ACFM4S_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6051243.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2273..6664
                     /gene="app"
                     /locus_tag="ACFM4S_001598"
     CDS             2273..6664
                     /gene="app"
                     /locus_tag="ACFM4S_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6051244.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTKGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYTDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKNWFYDEIFTGDTHSVFYEPHQNGKYTFHD
                     NNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNRHSLSFHRIQNTDEGAMIVNHNATTTST
                     VTITGNESITQPSGKNINRLNYSKEIAYNGWFGEKDATKTNGRLNLVYQPAAEDRTLL
                     LSGGTNLNGNITQTNGKLFFSGRPTPHAYNHLNNHWSQKEGIPHGEIVWDNDWINRTF
                     KAENFHIQGGQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCV
                     ENTITDDKVIASLTNTDISGNVDLADHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQ
                     RGNLSLVGNAQATFNQATLNGNTSASGNASFNLSNNAVQNGSLTLSGNAKANVSHSAL
                     NGNVSLADKAVFHFENSRLTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITL
                     NSAYRHDAAGAQTGSVSDTPRRRSRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGT
                     FRFMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLN
                     FTLQNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDA
                     LIAAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPA
                     TTAFPRARRARRDLPQPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDE
                     LDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSH
                     NRTENTFDDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRV
                     LHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLSFNRYRAGIKAD
                     YSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFM
                     LSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            complement(6980..>7198)
                     /locus_tag="ACFM4S_001599"
     CDS             complement(6980..>7198)
                     /locus_tag="ACFM4S_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6051245.1"
                     /translation="VQIVRQGEATPYWFKFNPLYVVEQSFGTLHRKFRYARAAYFGLS
                     KVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT         1912 a         2077 c         1746 g         1463 t
ORIGIN      
        1 aaccaaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc ggacaccgcc
       61 acgatgcttt cgatccgttt attttccgat acaaaacctc gaaaaaatca cgccgagcag
      121 gtcgtccgcc gtaaactcgc ccgtgatttc gccgcatgcg acctgcgcca agcgcaagtg
      181 ttcggcaaac agctcgattt gatggttgcc gcacaatgcc gccagcgaca attcttcctg
      241 cgctgctttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt cgccctcgcc
      301 ctgccatccg gcctcgcgca acaacgtccg tttcagcgcg tccaagccgt cgccggtttt
      361 cgccgacagc gcgatgacgg tttccgcgcc cgtaccgaac ccgcctgccg cgtgtgcgtg
      421 caaatcggat ttgctgtgga tttcgatgcg tttcaactcc ggcggcaacg cgtccaaaat
      481 cgcccgtgtc ttgtcgttca cgccttcgcg cggatcgacc aacaccagcg cgacatcggc
      541 ttcggatacg gctttgcggc tgcgttcgat gccgatgcgt tcgaccacgt cgtccgtctc
      601 gcgcaaacct gccgtatcga caatatgcac cggaacgccg tcaatcagga tacgttccct
      661 gaccgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt cgcccgccag
      721 cgcgttcaac aggctggatt tgccgacatt cggcgcgccg accaatacga cattcagacc
      781 ttcgcgcaaa atcgcgccct gctgcgcgtt ggcaagcaca tcatccacgg cgcggcgcaa
      841 gccgtccagt ttgccgcgtg cgtctgccgc ttcgagaaaa tcaatgtctt cctcgggaaa
      901 atctaacgtc gcttcgacca gcatccgcaa ggtaatcaag tcttcgacca gaccgtgtat
      961 ccgccgcgaa aaatcgcctt tgagcgagcg caacgccaga cgcgccgccg aacggctgga
     1021 tgcgtcaatc aaatccgcca cgccttccgc ctgtgccaag tccagtttgt cgtttaaaaa
     1081 cgcacgcttc gtaaactcgc ccggttcggc aaggcgcgcg cccaattcca aacagcggtt
     1141 cagcagcata tccatcacta ccggcccgcc gtgtccctga agctcgataa catcttcgcc
     1201 cgtaaaactt gccggtgcgg caaaaaaaag caaaagcccg ctgtcgattg cctgaccgtc
     1261 cgcgtccgta aaatcagcat aggttgccac gcgcggcttg ggcgttttcc cacacaaagc
     1321 ctctgccatc ggcagcaggt ttttccccga tatgcgtatc acgcccacgc cgccgcgccc
     1381 gggtgcggta gcgactgccg caatcgttgg aacgttatcc gacataaaac ccccgaaaat
     1441 tcaaaacagc cgcgattata gcaaatgccg tctgaagtcc gacggtttgg ctttcagacg
     1501 gcataaaacc gcaaaaatgc ttgataaatc cgtccgcctg acctaatata accatatgga
     1561 aaaacgaaac acatacgcct tcctgctcgg tataggctcg ctgctgggtc tgttccatcc
     1621 cgcaaaaacc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     1681 tcaacgcgcc atagagaaag cgcgcaaatg accgaaaacg cacaggacaa ggcgcggcag
     1741 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     1801 gtgcaaataa tgatagcccg gcgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     1861 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     1921 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     1981 ctgtacggct tcatcagcgt catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2041 ggctaccccg caaccgccgc ctcccttgcc ggcggcacag tggttgcctt ggcgggtgct
     2101 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2161 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgcgcgcc gcccggaagc
     2221 ggaagccgcg ccctcccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     2281 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggcc gcatccgctt
     2341 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cttgggcggg
     2401 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     2461 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     2521 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     2581 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggttata acaatgttga
     2641 ttttggtgcg gagggaagca atcccgatca gcaccgtttt tcttaccaaa ttgtgaaaag
     2701 aaataattat aaagcaggga ctaaaggcca tccttatggc ggcgattatc atatgccgcg
     2761 tttgcataaa tttgtcacag atgcagaacc tgttgagatg accagttata tggatgggtg
     2821 gaaatatacc gatcaaaata attatcctga ccgtgttcga atcggagcag gcagacaata
     2881 ttggcggtct gatgaagatg aacccaataa ccgcgaaagt tcatatcata ttgcaagtgc
     2941 gtattcttgg ctcgttggtg gcaatacctt tgcacaaaat ggatcaggtg gtggcacagt
     3001 caacttaggt agtgaaaaaa ttaaacatag cccatatggt tttttaccaa caggaggctc
     3061 atttggcgac agtggctcac caatgtttat ctatgatgcc caaaagcaaa agtggttaat
     3121 taatggggta ttgcaaacag gcaaccccta tataggaaaa agcaatggct tccagctagt
     3181 gcgtaaaaat tggttttatg acgaaatttt taccggagat acccattcag tattctacga
     3241 accacatcaa aatgggaaat acacttttca cgacaataat aatggcacag gaaaaatcaa
     3301 tgccaaacat gaacacaatt ctctgcctaa tagattaaaa acacgaaccg ttcaattgtt
     3361 taatgtttct ttatccgaga cagcaagaga acctgtttat catgctgcag gtggtgtcaa
     3421 cagttatcga cccagactga ataatggaga aaatatttcc tttattgacg aagggaaagg
     3481 cgaattgata cttaccagta acatcaacca aggtgctgga ggattatatt tccaaggaga
     3541 ttttacggtc tcgcctgaaa acaacgaaac gtggcaaggt gcgggcgttc atatcagtga
     3601 agacagtacc gttacttgga aagtaaacgg cgtggcaaac gaccgcctgt ccaaaatcgg
     3661 caaaggcacg ctgcacgttc aagccaaagg ggaaaaccaa ggctcgatca gcgtgggcga
     3721 cggtacagtc attttggatc agcaggcaga cgataaaggc aaaaaacaag cctttagtga
     3781 aatcggcttg gtcagcggca ggggtacggt gcaactgaat gccgataatc agttcaaccc
     3841 cgacaaactc tatttcggct ttcgcggcgg acgtttggat ttaaacaggc attcgctttc
     3901 gttccaccgt attcaaaata ccgatgaagg ggcgatgatt gtcaatcata atgccacaac
     3961 aacatccacc gttaccatta cagggaatga aagtattaca caaccgagtg gtaagaatat
     4021 caatagactt aattacagca aagaaattgc ctacaacggt tggtttggcg agaaagatgc
     4081 gaccaaaacg aatgggcggc tcaaccttgt ttaccagccc gccgcagaag accgcaccct
     4141 gctgctttcc ggcggaacaa atttaaacgg taacatcacg caaacaaacg gcaaactgtt
     4201 tttcagcggc agaccaacac cgcacgccta caatcattta aacaaccatt ggtcgcaaaa
     4261 agagggcatt ccgcacggag aaatcgtgtg ggacaacgac tggattaacc gcacgtttaa
     4321 agcggaaaat ttccatattc agggcgggca ggcggtgatt tcccgcaatg ttgccaaagt
     4381 ggaaggcgat tggcatttga gcaatcacgc ccaagcagtt ttcggtgtcg caccgcatca
     4441 aagccacaca atctgtacac gttcggactg gacgggtctg acaaattgtg tcgaaaacac
     4501 cattaccgac gataaagtga ttgcttcatt gaccaatacc gacatcagcg gcaatgtcga
     4561 tcttgccgat cacgctcatt taaatctcac agggcttgcc acgctcaacg gcaatctgac
     4621 cgcaggcgga gacacgcact atgaggttac gcacaacgcc acccaaagag gcaaccttag
     4681 cctcgtgggc aatgcccaag caacatttaa tcaagccaca ttaaacggaa acacatcggc
     4741 ttcgggcaat gcttcattta atctaagcaa caacgccgta caaaacggca gtctgacgct
     4801 ttccggcaac gctaaggcaa acgtaagcca ttccgcactc aacggcaatg tctccctagc
     4861 cgataaggca gtattccatt ttgaaaacag ccgccttacc ggacaaatca gcggcggcaa
     4921 ggatacggca ttacacttaa aagacagcga atggacgctg ccgtcaggca cggaattagg
     4981 caatttaaac cttgacaacg ccaccattac actcaattcc gcctatcgcc acgatgctgc
     5041 aggcgcgcaa accggcagtg tgtcagacac gccgcgccgc cgttcgcgcc gttcgcgccg
     5101 ttccctatta tccgttacac cgccaacttc ggcagaatcc cgtttcaaca cgctgacggt
     5161 aaacggcaaa ttgaacggtc agggaacatt ccgctttatg tcggaactct tcggctaccg
     5221 cagcgacaaa ttgaagctgg cggaaagttc cgaaggcact tacaccttgg cggtcaacaa
     5281 taccggcaac gaacctgcaa gcctcgaaca attgacggta gtggaaggaa aagacaacaa
     5341 accgctgtcc gaaaacctta atttcaccct gcaaaacgaa cacgtcgatg ccggcgcgtg
     5401 gcgttaccaa ctcatccgca aagacggcga gttccgcctg cataatccgg tcaaagaaca
     5461 agagctttcc gacaaactcg gcaaggcaga agccaaaaaa caggcgggaa aagacaacgc
     5521 gcaaagcctt gacgcgctga ttgcggccgg gcgcgatgcc gtcgaaaaga cagaaagcgt
     5581 tgccgaaccg gcccggcagg caggcgggga aaatgtcggc attatgcagg cggaggaaga
     5641 gaaaaaacgg gtgcaggcgg ataaagacag cgccttggcg aaacagcgcg aagcggaaac
     5701 ccggccggct accaccgcct tcccccgcgc ccgccgcgcc cgccgggatt tgccgcaacc
     5761 gcaaccccaa ccgcaacccc aaccgcagcc ccaaccgcag cgcgacctga tcagccgtta
     5821 tgccaatagc ggtttgagtg aattttccgc cacgctcaac agcgttttcg ccgtacaaga
     5881 cgaattagac cgcgtatttg ccgaagaccg ccgcaacgcc gtttggacaa gcggcatccg
     5941 ggacaccaaa cactaccgtt cgcaagattt ccgcgcctac cgccaacaaa ccgacctgcg
     6001 ccaaatcggt atgcagaaaa acctcggcag cgggcgcgtc ggcatcctgt tttcgcacaa
     6061 ccggaccgaa aacaccttcg acgacggcat cggcaactcg gcacggcttg cccacggcgc
     6121 cgttttcggg caatacggca tcgacaggtt ctacatcggc atcagcgcgg gcgcgggttt
     6181 tagcagcggc agcctttcag acggcatcgg aggcaaaatc cgccgccgcg tgctgcatta
     6241 cggcattcag gcacgatacc gcgccggttt cggcggattc ggcatcgaac cgcacatcgg
     6301 cgcaacgcgc tatttcgtcc aaaaagcgga ttaccgctac gaaaacgtca atatcgccac
     6361 ccccggtctt tcgttcaacc gctaccgcgc gggcattaag gcagattatt cattcaaacc
     6421 ggcacaacac atttccatca cgccttattt gagcctgtcc tataccgatg ccgcttcggg
     6481 caaagtccga acacgcgtca ataccgccgt attggctcag gatttcggca aaacccgcag
     6541 tgcggaatgg ggcgtaaacg ccgaaatcaa aggtttcatg ctgtccctcc acgctgccgc
     6601 cgccaaaggc ccgcaactgg aagcgcaaca cagcgcgggc atcaaattag gctaccgctg
     6661 gtaaccgccg gatatgccga aaggggtctg acgatgccac cgtgcgctgt caaacccttt
     6721 ttctgccgcc gccttgtgcc tgctccattg tctgataacc gtcaaattga tttttagccc
     6781 atgtttggtg ggtcggggaa atctatatct tcgtccgtgc cgaaatagtc tgagaccttt
     6841 gcaaaattcc tttccctccc gacagccgaa acccaaacac aggttttcgt ctattttcgc
     6901 cccaaatacc tcctaattct acccaaatac ccccttaatc ctccacggac acccgataat
     6961 caggcatccg ggctgctttt taggcggcag cgggcgcact tagcctgttg gccgctttca
     7021 aaaggttcaa acacatcgcc ttcagatggc tttgtgcact cactttgctc agcccgaaat
     7081 aggctgcccg cgcatagcgg aatttacggt gcagcgtacc gaagctctgt tcgaccacat
     7141 atagtggatt aaatttaaac cagtacggcg ttgcctcgcc ttgccgtact atttgtac
//
LOCUS       DAXHMX010000108         7150 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHMX010000108 DAXHMX010000000
VERSION     DAXHMX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.108"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            152..1090
                     /locus_tag="ACFM4S_001600"
     CDS             152..1090
                     /locus_tag="ACFM4S_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6051246.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            1219..1512
                     /gene="hfq"
                     /locus_tag="ACFM4S_001601"
     CDS             1219..1512
                     /gene="hfq"
                     /locus_tag="ACFM4S_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6051247.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            1596..2162
                     /locus_tag="ACFM4S_001602"
     CDS             1596..2162
                     /locus_tag="ACFM4S_001602"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6051248.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRFILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAVGQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            2216..2590
                     /locus_tag="ACFM4S_001603"
     CDS             2216..2590
                     /locus_tag="ACFM4S_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6051249.1"
                     /translation="MQYLFVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            2600..3094
                     /gene="folK"
                     /locus_tag="ACFM4S_001604"
     CDS             2600..3094
                     /gene="folK"
                     /locus_tag="ACFM4S_001604"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6051250.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(3222..3701)
                     /locus_tag="ACFM4S_001605"
     CDS             complement(3222..3701)
                     /locus_tag="ACFM4S_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6051251.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(3921..4658)
                     /gene="ubiE"
                     /locus_tag="ACFM4S_001606"
     CDS             complement(3921..4658)
                     /gene="ubiE"
                     /locus_tag="ACFM4S_001606"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6051252.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(4701..5069)
                     /locus_tag="ACFM4S_001607"
     CDS             complement(4701..5069)
                     /locus_tag="ACFM4S_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC6051253.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            5189..6808
                     /gene="mnmC"
                     /locus_tag="ACFM4S_001608"
     CDS             5189..6808
                     /gene="mnmC"
                     /locus_tag="ACFM4S_001608"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6051254.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQHGVW
                     LNLPAFVRALLRHPLIGLHEDTPLTGISHDGEKWIASTPNGIFSATHIVYCTGAHSPC
                     LPEINLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(6922..7039)
                     /locus_tag="ACFM4S_001609"
                     /pseudo
     CDS             complement(6922..7039)
                     /locus_tag="ACFM4S_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         1902 a         2234 c         1450 g         1564 t
ORIGIN      
        1 ttccgattta aaaacagaat gttataaaac ccaatcccca aaacacaaca agaccgcccg
       61 cctacgggca gtctcctgtc agacgacata ctttacagat ggctgttttt tcaacaaaat
      121 aacgccaata ctcaaaaata tggaatcaaa aatgtccatc catactctga aacgcctgcc
      181 ctcatcgctg ctgctcggtc tctgcctttc cctgccgtca gcccaccttt ttgccgacaa
      241 cgacatttta gggcaatttt tagaacagaa catgcttacc tcctccgatc cgatagaaat
      301 attcgccgaa agcacgatac accccaccaa cacccaagcc attacaggcg gtctgattct
      361 ctcctcacag tctgccctgg tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa
      421 cgccgacagg attatgccca tcgcctccat ttccaaactg atgagcgcga tggtcgtttt
      481 ggatgcaaac ttggacatga acgaaaccgt taccattacg cccgacgaaa tcgaccgcat
      541 caaagggacc ggcagccgtc ttgccatagg tacggcactt acacgcaaag aactgctgca
      601 cctgagcctg atgagcagcg aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg
      661 cggcatgggc gcatttgtcg ccgccatgaa ccgcaaagcc caaagcctcg gtatgtacgg
      721 cagccgcttt tacgaaccga ccggactcaa cttccaaaac gtttctaccg ccaaagacct
      781 gagccttatg gtcaacgccg ccgcccaata tccgcaaatc cgcaccaact cgacttccaa
      841 ctacgcctcg gtacagacca aaaacgggca gcagaactac aaaaactcca atgccctggt
      901 cagagaaggc atgtggaaca tcgaattgca gaaaaccggc tacatacgcg aagcaggcag
      961 gtctatggtt gtcaaagcca acattcaaaa tcaacccgtt accatcgtat tgctgaactc
     1021 gcccacatcc gccacacgcg tcaacgacgc ccgcaaaatc gaatcgtgga tgctgcagca
     1081 acgctcctga catacaaatg cccggcggaa aaccgcccaa acacaaaaaa aattggtaat
     1141 ttttcctatt caaggctaca attcgacacg cacagggcgt ctgcccgatt tcacaatcat
     1201 aataacggag ttaaaaatat gacagctaaa ggacaaatgt tgcaagatcc cttcctgaac
     1261 gccctgcgta aagagcacgt tccggtttcg atttacctgg tcaacggcat caaactgcaa
     1321 ggtcaggttg aatctttcga ccaatacgtc gttcttctga gaaacacttc cgtaacccaa
     1381 atggtttaca aacacgccat ttccaccatc gtaccggctc gctccgtcaa cctccaacac
     1441 gaaaacagac cccaagccgc accggcttcg acccttgtcc aagtggaaac cgtccagcag
     1501 cctgccgaat aatccgcgcg aagcatgacg tgtcatatct ttcaatacct taccgacatc
     1561 ggtaaggtat ttttattttc agacagcatt taaaaatgtt attgaaaaac atccttccat
     1621 tcgcccattg ccttttgcgg caggcacttc ccgaaggcgg caatgcgctg gacggcaccg
     1681 ccggcaacgg acacgacacc cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt
     1741 gggcattcga catccagccg caagccctga acaacacccg atgccgtctg caggaagcag
     1801 gttacagcaa tgtacggttc atcttggacg gacatgaaaa cctgaagcaa tatattccaa
     1861 agccgctgga tgcagccatt ttcaatttcg gctggctgcc cggcggggac aaaagcctta
     1921 ccacccgcac ggaaaccagc attgccgccc tttctgccac cttatcccta ctgaaagaaa
     1981 acggtatgct tattgccgtc ctctatccgg gacacgaaaa cggcaaacag gaggcagaag
     2041 ccgtcggaca atgggcaaaa aacctgcctc aagaacagtt tgccgtttta cgttacggct
     2101 ttaccaaccg gaaaaacagc ccaccctatc tgttggcatt tgaaaaactg cgtcaaaaat
     2161 aactgtttgc ggtaaaataa gccgattcca acccttattt tgataagagc cgaccatgca
     2221 gtatctgttt gtcaaataca gccaccaaat cttcgttacc atcaccattt tggtattcaa
     2281 catccgtttt ttcctacttt ggaaaaatcc agaaaagccc ttggcgggct tttggaaggc
     2341 actgccacac cttaacgaca ccatgctgct gtttacggga ttgtggctga tgaaaattac
     2401 ccatttctcc ccgttcaacg cgccttggct cggtacaaaa atcctgcttc tgctcgccta
     2461 tatcgcattg ggtatgatga tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt
     2521 ttacctgctc gccatgtgtt gcgtcgcctg catcgtttac cttgccaaaa ccaaagtcct
     2581 gcctttctga aacaccgtta tgaacaacag acattttgcc gtcatcgccc tgggcagtaa
     2641 tcttgaaaac cctgcccaac aggtacgcgc cgcattggac acgctgtcgt cccatcctga
     2701 catccgtctt aaacaggctt cctcactgta tatgaccgcg cccgtcggtt acgacaatca
     2761 gcccgatttt gtcaatgccg tctgcaccgt ttccaccact ctggacggca ttgccctgct
     2821 tgccgaactc aaccgtatcg aggctgattt cggacgcgaa cgcagcttcc gcaacgcgcc
     2881 gcgcacattg gatttggaca ttatcgactt tgacggcatc tccagcgacg acacccgact
     2941 caccctgccg catccgcgcg cgcacgaacg cagtttcgtc atccgccctt tggcagaaat
     3001 cctccctgat tttgttttag gaaaacacgg aaaggttgcc gaattgtcaa aacggctggg
     3061 caatcaaggt atccgtcttt tacccgacag gtaattccgc acgcggatgc cgtctgaatg
     3121 ccggtttcag acggcatttt taccctgccg ccaatctgta cgcaaaaaaa atcgcccctt
     3181 gaaaaagggg cgcaaaagga acacaaacca ctaccaaaac tttaaatctg caacactgcc
     3241 tgccgcatac tgtatccgac aggatataaa gccctcacta aatcgtttcg agaaatccaa
     3301 acttcttcat cgccgacaga aaatctgcct ttctccggca ccagctccaa caaaaacggt
     3361 tgaaccgccg tatgcagcct gtccttacac cgccccagct ttctggacaa cgcggacaag
     3421 gcacgcttat agacactatc cttgcggtca agttcccgcg cactgccgac ccagctcaaa
     3481 cgaaggttgc ggctttcatc ctcaatcagc aaaatgcctg ttttgacttc ccccacctcg
     3541 ggctcacatg aaagcgcaat ataatatttg ccgtcgatgt tgacatacgc cgcttcatgc
     3601 acctgacagt tgcagccgga agtggcaagc agcgaataaa cagagtcccg atgcagtttc
     3661 aaaatatcaa gggaaatctg tttgaaaata ctttccggca tttttccctc ctttcgtctg
     3721 ctgcgttgtt tactggattg actgcattat acagacatct cccaatcttg caaatgataa
     3781 ttgttcctat tttttaaaaa atttataaat tattgatgga tatgtgaatt aattttaaga
     3841 tttaaaatct ctttctcaaa aaaacacaaa aatgccgtct gaaacgtgtt cagacggcat
     3901 tggctgcaca gccagtccgt ttaaaatttc acgcccttat gcagcgcgac gatgcccgca
     3961 ctcatattgt gataatccac gctgtcaaag cccgcatcca gcatcatctg tttcaaagtt
     4021 tcctgatcgg ggtgcatacg gatggattcg gcgagatatt gataactctc cgcatctttg
     4081 gcaatcagtt tgcccatgac cggcagcagc ttgaacgaat agaaatcata cgcgccttcc
     4141 aaaggtttgt agattttgga aaactccaat accagcaatg tcccgccggg tttcaaaaca
     4201 cggtacatct ctttcagcgc ggcatcttta tgcgtcatat tccgcaaacc gaacgccacg
     4261 gaaaccaagt tgaaataatt gtcagggaaa ggcagttttt ccgcatcggc aagcgacacg
     4321 ggcaaaatca tgccttcgtt caaaagacgg tcgcgcccga cggtcagcat agaggaatta
     4381 atatcggtca gccaaacctc gccttccttg ccgacccgtt tcgcccaacc gcgcgacaaa
     4441 tcgcccgtac cgccggcaat gtccaacact ttatcgcctt ttttcaggtg cgccgtgttg
     4501 atggtgaaat gcttccacac cctgtgcagc cctgccgaca tcacatcgtt cataatgtcg
     4561 tagtttttgg cgacggagtg gaacacttcc gccacttttc cggctttctc atcttcgttg
     4621 accgtactga atccgaaatg cgttttctgt ccgcccatat tctaaccacc tccctgatgc
     4681 cgccctacgg catcccaaac ttaacaatct gattgctttt caaaacggga agcacccgcc
     4741 gccgccaatt tgtccaaata ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc
     4801 agatacgccc aatcgtaaag accgctgtcg tgcccgtctg aaaaactgat tttcagcgcg
     4861 tactgtccga caggctgcaa atccgcgatt tggacatccg ccttgccggt ctgcaaaaca
     4921 tcctgtccca cgccgtgtcc gcgcacttcc gcactcggcg aatagacgcg taaaaattcg
     4981 gcaggcagat ttttaggttc gtccccataa gtcaaaacca acacggtacg gtttttcaac
     5041 aaacggattt caacaggaat attattcaaa aaacacctcg gttcggcata caatatccgg
     5101 caggcgcagg cgtgccttca aacggcgcgt attttaacag ctttatcccg ataccgatat
     5161 tcaaacccac actttaaacg gcattgatat ggacaatctc gtatgggacg gcgttcccga
     5221 catccgcaca ctcgaccaaa ccatccgcaa acacgcacac ccgctcaacc tgattgtctg
     5281 cctccccgat aatcagattc ccgattttca aaccgcacaa gatgcttcgg acgcggaatg
     5341 ccgtctgaag caccgtttgg atcaggcaac gcagtgcctc cagttcgaca gcatcaacct
     5401 catcgaacac atcctgcccg atgtccgctt ctggctggtt cccccttcac gcacccgccg
     5461 cctgcacgaa cacttccacc acatttcctg gcagaccgaa gccatcccgc aaaccgaaag
     5521 caaacccgac aaaccctggt ttgcacttcc acaaacatcc gaacggcaaa aaccggaaca
     5581 catcctcgtt atcggcgcgg gcatatccgg cgcggcaacc gcccacgcct tagcatcaca
     5641 cggcatttcc gttaccgtat tggaagcccg aaaagccgcc caagccgcca gcggcaaccg
     5701 ccaagggctg ctctacgcca aaatctcgcc gcacgacacc gaacaaaccg aactgctgct
     5761 tgccggctac ggctacacca aacgcctgct cggacacatc ctgcccgaat ccgaaacctg
     5821 gggcggcaac ggcatcatcc acctcaatta cagccgcacc gaacaacaac gcaatcacga
     5881 attgggtttg caaaaacacc ataaccacct ctaccgcagc atcacgcagg cagaagccga
     5941 aaaaatcgcc ggtatcccgc tgaacacgcc ctacgccgaa ccattatgcg gactgttttg
     6001 gcagcacggc gtatggctca atctacccgc attcgtccgc gccctcctcc gccatccgct
     6061 gattggacta cacgaagaca caccgttaac cggtatttcc cacgacggag aaaagtggat
     6121 tgcaagcacg ccaaacggca tcttttctgc cacacacatc gtctactgca ccggcgcgca
     6181 cagcccctgc ctgcccgaaa tcaacctcgc cgccctgccc ctcaggcaaa tacgcggaca
     6241 aaccggcctc acaccgtcca ccccgttttc cgaacaactg cgttgcgccg tttcaggcga
     6301 aagctacatc agcccgtcgt ggcacggact gcactgctac ggcgcgagtt ttattcccaa
     6361 cagcagccat accggatgga acgaagccga agaagcctca aaccgccaag cattggcaca
     6421 ccttaacccc gcccttgccg aatcattgtt tgccgccaac ccaaaccccc aaaaatacca
     6481 agggcacgcc gccatacgct gcgacagccc cgaccacctt cccctagtcg gcgcactcgg
     6541 cgacattgcc gccatgcggc agacctacac caaactcgcg ctggacaaaa actaccgcat
     6601 cgacacccca tgcccatacc tgcctaatgc ctacgtcaac accgcccacg gcacacgcgg
     6661 gcttgccacc gcccccatct gcgccgccgc cgttgcagcc gaaatcctag gcttgcccca
     6721 tcccctctca aaacgcctgc gccacgccct acaccccaac cgcaccatca tccgcgccat
     6781 cgtcagaagg aaggatctaa ccccttaagc ctttgccgca tgccgtcccg accacagaaa
     6841 atcatattca aataaacata ccaatcctga cggtaaaata tcgaaaacac ccataacgtt
     6901 ttcttcccaa aataaggagt aaggaatgcc taacaaattg cctttgctgt ctaagatgaa
     6961 tgttccgtaa cattcgaaga cttccttaac cctgccagcc gtttcggttt ctgtaccccc
     7021 tgttagataa ggaaggaatg aaaaagccct gttgattgta tgaagtaaag ggcgttaatt
     7081 tttgttaatc ttcccttctc aggaactcaa tatataagga tgttaatttt tatttccctt
     7141 ctcgaggact
//
LOCUS       DAXHMX010000109         7096 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHMX010000109 DAXHMX010000000
VERSION     DAXHMX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.109"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            143..1993
                     /gene="ltgA"
                     /locus_tag="ACFM4S_001610"
     CDS             143..1993
                     /gene="ltgA"
                     /locus_tag="ACFM4S_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6051255.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2282..2494
                     /gene="rpsU"
                     /locus_tag="ACFM4S_001611"
     CDS             2282..2494
                     /gene="rpsU"
                     /locus_tag="ACFM4S_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6051256.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2626..3072
                     /locus_tag="ACFM4S_001612"
     CDS             2626..3072
                     /locus_tag="ACFM4S_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6051257.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3150..3392)
                     /locus_tag="ACFM4S_001613"
     CDS             complement(3150..3392)
                     /locus_tag="ACFM4S_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051258.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDV
                     IASALECNYGAANQAIERQRQRAAAVAVHERVAKFL"
     gene            complement(3397..3789)
                     /locus_tag="ACFM4S_001614"
     CDS             complement(3397..3789)
                     /locus_tag="ACFM4S_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6051259.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3861..4466)
                     /locus_tag="ACFM4S_001615"
     CDS             complement(3861..4466)
                     /locus_tag="ACFM4S_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6051260.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(4568..6244)
                     /locus_tag="ACFM4S_001616"
     CDS             complement(4568..6244)
                     /locus_tag="ACFM4S_001616"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6051261.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLACRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAANPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            complement(6405..7019)
                     /locus_tag="ACFM4S_001617"
     CDS             complement(6405..7019)
                     /locus_tag="ACFM4S_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6051262.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
BASE COUNT         1846 a         1952 c         1883 g         1415 t
ORIGIN      
        1 aatttaatcc actataaatt atgtcggaaa cattccaaag gcggtgcagt ttcggcatat
       61 aattcgggca aacgcctgtt cagacggcat tttgtctttt ccaaccctga ccgttcaggg
      121 ttccgattct taaggaaatc cgatgtacct accctctatg aagcattccc tgccgctgct
      181 ggcgacccta gtgcttgccg cgtgttcttc gacaaacaca ctgccagccg gcaagacccc
      241 ggcagacaat atagaaactg ccgacctttc ggcaagcgtt cccacccgcc ctgccgaacc
      301 ggaaggaaaa acgctggcag attacggcgg ctacccgtcc gcactggatg cagtgaaaca
      361 gaaaaacgat gccgccgtcg ccgcctattt ggaaaacgcc ggcgacagtg cgatggcgga
      421 aaatgtccgc aacgagtggc tgaagtcttt gggcgcacgc aaacagtgga cgctgtttgc
      481 acaggaatat gccaaactcg aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga
      541 ttcgagccgc aacgactata cgcgtgccgc cgaactggtc aaaaatacgg gcaaactgcc
      601 ttcgggctgc accaaactgt tggaacaggc agccgcatcc ggcttgttgg acggcaacga
      661 cgcctggagg cgcgtgcgcg gactgctggc cggccgccaa accacagacg cacgcaacct
      721 tgccgccgca ttgggcagcc cgtttgacgg cggtacacaa ggttcgcgcg aatatgccct
      781 gttgaacgtc atcggcaaag aagcacgcaa atcgccgaat gccgccgccc tgctgtccga
      841 aatggaaagc ggtttaagcc tcgaacaacg cagtttcgcg tggggcgtat tggggcatta
      901 tcagtcgcaa aacctcaatg tgcctgccgc cttggactat tacggcaagg ttgccgaccg
      961 ccgccaactg accgacgacc aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg
     1021 ttgggacgag ctggcctccg ttatctcgca tatgcccgaa aaactgcaaa aaagcccgac
     1081 ctggctctac tggctggcac gcagccgcgc cgcaacgggc aacacgcaag aggcggaaaa
     1141 actttacaaa caggcggcag cgacgggcag gaatttttat gcggtgctgg caggggaaga
     1201 gttggggcgc aaaatcgata cgcgcaacaa tgttcccgat gccgacgcaa acagcgtcct
     1261 ccgtatggcg gaagacggcg cgattaagcg tgcgctggta ctgttccaaa acagccggac
     1321 cgccggcgat gcgaaaatgc gccgtcaggc tcaggcggaa tggcgttttg ccacacgcgg
     1381 gttcgacgaa gacaagctgc tgaccgccgc acaaaccgcg ttcgaccacg gtttttacga
     1441 tatggcggtc aacagcgcgg aacgcaccga ccgcaaactc aactacacct tgcgctatat
     1501 ttcgccgttt aaagacacgg taatccgcca cgcgcaaaat gttaatgtcg atccggcttg
     1561 ggtttacggc ctgattcgtc aggaaagccg cttcgttata ggcgcgcaat cccgcgtagg
     1621 cgcgcagggg ctgatgcagg ttatgcctgc caccgcgcgc gaaatcgccg gcaaaatcgg
     1681 tatggatgcc gcacaacttt acaccgccga cggcaatatc cgtatgggga cgtggtatat
     1741 ggcggacacc aaacgccgcc tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc
     1801 cggtcccggc agggcgcgcc gatggcaggc ggacacgccc ctcgaaggcg cggtatatgc
     1861 cgaaaccatc ccgttttccg aaacgcgcga ctatgtcaaa aaagtgatgg ccaatgccgc
     1921 ctactacgcc tccctcttcg gcgcgccgca catcccgctc aaacagcgta tgggcattgt
     1981 ccccgcccgc tgacgtaccg atgccgtctg aaacccgccc ggtctttcag acggcatttt
     2041 tatcccgaac ggcattgacg gcgaaccata aatataagac aatccgaaaa ttgtttttcc
     2101 tgctttttca agcagcttga cacggcacaa gccgacccgt taggaggtga tgtttccgtc
     2161 acggcgcgta tcccgccgcc gcaaggcaca gcgatacggt aaaccttcaa caccgtctgc
     2221 cctacccttt ccaccgatat gatgggcaga tgaaacaacc gaatttatta aaggaaataa
     2281 aatgcctgca atccgcgtaa aagagaatga accatttgaa gtcgctatgc gccgtttcaa
     2341 acgcgccgta gaaaaaaccg gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa
     2401 accgactacc gaacgcaaac gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct
     2461 gcgcagccaa caactgccgc ccaaaatgta ctaaacgttc aagtacagat tacaggtcag
     2521 ccctgtgata tgaggacaca ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg
     2581 gcatcgaaac ccgccgtttc catccgacat cccagcgagg acatcatgag cctgaaaatc
     2641 cgccttaccg aagacatgaa aaccgcgatg cgcgccaaag accaagtttc cctcggcacc
     2701 atccgcctca tcaacgccgc cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac
     2761 gatgccaaaa tcaccgccat cctgaccaaa atggtcaaac agcgaaaaga cagcgcgaaa
     2821 atctacactg aagccggccg tcaggatttg gcagacaaag aaaacgccga aatcgaggta
     2881 ctgcaccgct accttcccca aatgctctcc gccggggaaa tccgtactgc ggtcgatgcc
     2941 gccgttgccg aaaccggcgc ggcaggtatg gcggatatgg gcaaagtgat gggcgtattg
     3001 aaaacccgcc tcgccggcaa agccgatatg ggcgaagtca acaaaatcct gaaagccgtg
     3061 ctgaccgcct gattgcccga atatcggaca aaatgccgtc tgaagcccgt atcgcaggtt
     3121 cagacggcat tttcatggtt tgatgatatt tataaaaatt ttgcaacacg ctcatgcaca
     3181 gcaactgccg ctgctcgttg gcgttgccgc tctatcgctt ggttagctgc accataattg
     3241 cactctaatg ctgatgcaat aacgtcccaa tatcgaatcg gcaggggcaa cacggtttcg
     3301 atacaccgaa acgggttttg ccgataaacg gattccgttt gcgccccatc ggacaaaatg
     3361 ccgtctgaaa cacgattccg ttcagacggc atagatttat ttgaccaatt tcaagccttt
     3421 tttggcgggc cggggcgcgg tttcggcaga ggtgttttca ggcggcgtgt cggggcgata
     3481 cgcttccaat tcaaacccca taccttcgcc ggtctcccgt gcgaaaaggc tgaggacgtg
     3541 tccgacaggt atccatatgt cgtgcgcctg tccgccgaag cgggcggaaa agctgatcca
     3601 atcgttgtcg atttgaaggt tttgcgtggc ggtcgcgccg atgttgagca taatttcgtt
     3661 gtcgcggacg tactgcatgg ggacacgcgt gtgttcgttg acccagacaa gaatgtgcgg
     3721 cgtgaggctg ttatcgctgc accattcgca aagtgcgcgg aggatgtagg gtttggtgga
     3781 agtgggcata atgggttccg tgtggtgtgc ctgaatgtga aaatgcctac aaaacggcgg
     3841 gttttgcaag cattccgggc ttatttgcgc atggcttttt cggcgggtgt cagtgcttcg
     3901 ataaaggctt cgcgctggaa gatgcgctcg gcgtatttga gcagcggcgc ggcacttttg
     3961 cccagtttga catcgtagtg gtcgagccgc cacagcagcg gagcaagggc gacatcaatc
     4021 atagaaaaat cttcgccgag gatgtatttg cttttgctga acgaaggggc aagcatggtc
     4081 agaccgttgc cgatggcttc gcgcgctttt gcctgttcct tgttggtggc ggcggggttt
     4141 tctaacactt ggacgtggtt gaacaattct ttttccatac ggtacagcac cagccggccc
     4201 cggccgcgca taacgggatc gccgggcata agctgcggat gagggaagcg ttcgtcgatg
     4261 tattcgttga tgatgttgga ctcgtgcagc accaaatcgc gctcgaccag cacgggaact
     4321 tggttatacg gattcatgac ggcgaggtct tcgggtttgt tgtaaatatc gacgtctttg
     4381 atttcaaaat ccataccttt ttcgtacaaa acgaagcggc agcggtggct gaaggggcag
     4441 gtaatgccgg aatagagggt catcataata attgtcgctc ctgtgtgatg cctgcaaaac
     4501 ggctgattta taaaggttta atcgcgcaat tatacgcgat ttccggcact taatccagaa
     4561 attcggctca atctgttgtt tttaatatat tttccccgat tttccgtatc agtgcgaact
     4621 tactgtcttt gttgcgcggg cgcgcaccct gccagaccgt ctgccagcct tgcgacgcat
     4681 ccgcattttg gggcaagcgg acgatacggt agcggcattg tacatcgccg acgcggtgcg
     4741 gcaatgtgcc gtactgcgtc caaacaatcc gcgtgtgcag gtcgccgccg cctatgtcga
     4801 tacactcgat gccgtctgaa agctcccgtt ttaattccgg ggaaagcgat gcctccatac
     4861 tccggacgac cggcgcgtgg cttttcgccg cgtccagcca cggcaggaac agcgtcatca
     4921 gcaaagccca ggtcagggta acgcctgccg cccagttggt aaccgcctgc ctgccgcgta
     4981 tgtttttccg ggtaatcgcc cacagccaca agggtgtgaa cagtacggca accgccatcg
     5041 gaatgggatc gatatcagga acataatacg ggctgaaata ggcggcgcgt tcggcaagct
     5101 tggcgggcca gccgtaattc atggcgaaaa agcccgtcca caggaacacg gcaaacagtc
     5161 cgaacgccat aatgccgaac cagttgacaa acgccgccgc gccgcgcctc aggctgtcca
     5221 gttgcgccgc gccgaacagg gcaagcggcg gcagcagcca gacgaggttg tcctgaaaac
     5281 gctgcggatt ggcggcgagc agcaccaaaa cggcaagcat ccacacaacg cccaaaatcc
     5341 cccagtcggt cgaaaacagg cgcgtgcggc aaaccgtcca aaccgccagc ggcagcgcgg
     5401 gcagtgcaaa ccaaagcagg tttttcagat agtaaaacaa actgaatgcc gtctgaacgt
     5461 gccgcacgcc gccgaacgta ccgaaaacgt gatcgtcgag ccattgcgcg aacagcgcgg
     5521 gctgcgtttt tgccaagagc agcgggtaaa cggtcatcag cggcagggca aaggcaagcg
     5581 aggcgactgc cgtcaacatc aaacgcctgc tttgccacgg acggaaaaac atcagcactg
     5641 gcaggggcag catcagggca aatgctgccg gataagctgc tgccaacgac atcagcgtcc
     5701 aacccgtacc gagcagaaaa gaggcggcaa tcacacggcg gcacgccagc gagtagccgt
     5761 gcagcaccag tccggcggcg gcaaaggcgg cggcagcggg attgagaaag tgtacggtcg
     5821 gaatcagccc gatacagccg ataaggatta aaacaacgct gcgcccgtgg tgtctgccca
     5881 aaaagttgaa accggcaaag ccgcaggaag tcagtccgac aacggcgaaa aacacgcctg
     5941 caaagcgtgc ggcatcgtat gagtcggcag cccacggcga cagcaaatgt ttgaacgcgg
     6001 cggcaaccca aagatacacg ggcggtatgc cgaaatcggt ttgaccgaac agatgggcaa
     6061 ccaagggggt ggggctgcct gccagtgctt cgacggcggt atagacggca ggttcggcag
     6121 gattccataa atcatgtgaa aatacacccg gccacaacca ggcaaacgcc atcaacagca
     6181 gcagccacgg cttttcgtgg gttttggcgg gcgggcgggc atcgggcggg gtataggtca
     6241 gcataagcgt gagaaaaaat ggacggattg ccaagtgtag caaatattcg cacaaaggtc
     6301 gtgcagagac tgcttcagac ggcatcagac acaaaaagac cggcaacaaa aaagactgca
     6361 catggcagtc tttgcagata ctatcttttt cataatattt tttcctagcc caacacagtc
     6421 catagcatat caaaactgtt gttttcaacc aggatatata tccccaatcc taaataaaca
     6481 acagcaacaa accatctgct atatttttcc aaagtttctc caacagaagg gacttgtgct
     6541 aatttttggg cagaaaaaac caagagataa atcatgacta gaaaggtaag taaagccact
     6601 atcaaattcg ctaaatttaa ggtagtaaaa tatgggacaa agacaccaat attgtcagca
     6661 ccacaacttg caaaagtaat catagcgact agaaaaatca ggtttttatt atctttgcgc
     6721 aaaccctctt tggcaatagc ctctccatca gaatctccta acagcaaaac tttgatgcct
     6781 aggaaaattg gaatcaagcc gagcaaacct aaaatctctt tactaggaat ataatctaag
     6841 acaaatgcaa aaagcaaact tagcaatatc aggctaacag agcctagaaa ttgtcctaaa
     6901 tagatgttaa tgatgtcttt tctgcttttt cttttggcaa aaaataacat taggataata
     6961 agtaagtcta cggctgtccc agaatacagg attattgaag taacaacatt ttgaatcata
     7021 aaacatctca ttcaaatata tttttaaatg tattcaaaca ttaaaccttg tagatgtcaa
     7081 cttcaacccc gtcaaa
//
LOCUS       DAXHMX010000110         7084 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHMX010000110 DAXHMX010000000
VERSION     DAXHMX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.110"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            506..952
                     /gene="yciA"
                     /locus_tag="ACFM4S_001618"
     CDS             506..952
                     /gene="yciA"
                     /locus_tag="ACFM4S_001618"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6051263.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
     gene            complement(1003..1824)
                     /locus_tag="ACFM4S_001619"
     CDS             complement(1003..1824)
                     /locus_tag="ACFM4S_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6051264.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            2094..2552
                     /locus_tag="ACFM4S_001620"
     CDS             2094..2552
                     /locus_tag="ACFM4S_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6051265.1"
                     /translation="MNIQTRLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2716..3831)
                     /gene="lst"
                     /locus_tag="ACFM4S_001621"
     CDS             complement(2716..3831)
                     /gene="lst"
                     /locus_tag="ACFM4S_001621"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6051266.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            4266..6491
                     /locus_tag="ACFM4S_001622"
     CDS             4266..6491
                     /locus_tag="ACFM4S_001622"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6051267.1"
                     /translation="MTQKSTIIYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVVDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKTGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAKSLAENEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQALST
                     L"
     gene            6634..6942
                     /locus_tag="ACFM4S_001623"
     CDS             6634..6942
                     /locus_tag="ACFM4S_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051268.1"
                     /translation="MKTVPTILISILLATALPASAHGIHKSKPLTMDELPPICQQYFK
                     RAETCYNKAGNKANFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRISNSNAV
                     "
     gene            6932..7084
                     /locus_tag="ACFM4S_001624"
     CDS             6932..7084
                     /locus_tag="ACFM4S_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051269.1"
                     /translation="MPSETMISDGILTSKRTSLIKPAFPASLTRTGLNEVTALTIMAS
                     SHSQKL"
BASE COUNT         1926 a         2087 c         1545 g         1526 t
ORIGIN      
        1 tccgacaaca ccgtaatctc gaaacccgtc attcccgata acagcgcaat cttgaaaccc
       61 gtcattcccg acaacaccgt aatctcgaaa ttcgtcattc ccgcgcaggc gggaatccag
      121 acctgtccgc acagaaactt atcggataaa aacagttgcc caaaccccgc gttctagatt
      181 cccacttccg tgggaatgac ggttcagttg cgttccgaca acaccgcaat ctcgaaaccc
      241 gtcattcccg cgcaggcggg aatccagacc tgtccgcaca gaaacttatc ggataaaaac
      301 agttgctcaa acccctcgtt ctagattccc acttccgtgg gaatgacggt tcagttgcta
      361 cggccccacc ctacaatacg ccttgcgaat ctgttccgcg cccgcccttg tatgtctcaa
      421 atgggttaaa ctacacactt cccgacttcc cgtttcgggc aggggtgcag gttcgattta
      481 tatttcaata aaacaaggaa actttatgca gcacgaagaa ggcaaccgcc aacgccctca
      541 aggcgaactg ctcctgcgta ccgtcgctat gccgcgcgat accaatccca accaagacat
      601 tttcggcggc tggattatgt cgcagatgga tttgggcggc ggcatattgg cggcggaaat
      661 cgcgcgggga cgcatcgtta ccgtcgccgt tcaggaaatg aacttcatcc gcccggtcaa
      721 ggtcggcaac gtcgtctgct gctacgggca ttgcgtccgc gtgggcaaca cttccctcca
      781 gcttaaagtc gacgtctggg tgaaaacttt gatgaacgat tgcgttaccg aagaccgcta
      841 cctcgtaacc gaagccgtgt tcacttatgt tgccatcgat gcggaaggca atccgcgccc
      901 gattccgaaa gaaggcaacc ccaaattggc gggcttattg cctactccgt aaaaataccc
      961 gtaaaaatgc cgtctgaaac tgtcttcaga cggcattttg catcatttga accaggacct
     1021 gatgcgggaa aacagcgaca tactctgttt ttcaaagtct tcccgcactt cgggcacggc
     1081 tttttttatc actttcattc ctgcaaaagt ctgcgccacg ggcataattt caatcgtgtt
     1141 cacattcata tgcgccggcc gctggtacag ccacaatgcg gtttccgcaa tatcttcggg
     1201 gcggataaat tccacaccct catacacgcc cgccgccctc tcgtcatcgc ctttgaagcg
     1261 cacattggaa aactccgtat tgccgcacaa acccggctcg atattggtaa cgcggatgtt
     1321 cttatccgcc aactccgcgc gcaaattcag gctgaactgc cgcacaaacg ccttggtcgc
     1381 cccgtaaacg ttgctgccgg cataagcata attgcctgca atcgaaccca aattcatcac
     1441 atagccgccg ccgcgttcca ccatttgcgg caaaattttg cgcgtcagga acgtcaaacc
     1501 caaaacattg gtttgaatca tcgtttccca atcttcaaaa tccgccttgt ccgccgtgtc
     1561 cagacccaaa gccagcccgg cattgttgat gaggcagtcg atgtcggaaa attcatcggg
     1621 gatgccgttt aaggcgtttt ccaccgactc gcgtcgcgac acgtccattt ccaaagggta
     1681 aaacaaagca cccaattcat ccgccaaggc ctgaagcctg tccgcacggc gcgccgcacc
     1741 gataacgcgg tatcccgccc cgacaaacgc gcggcacatc gcttcgccga atcctgccga
     1801 agcaccggta attaaaacag ccattgtttt tcctttcttt ctcgtttccc aaacctgttt
     1861 caggtatcat aacgctcttc agacggcatc gcgccggctg aaaatgacaa caaccatacc
     1921 accacgacag gacacattca atatgtttca acacacagga cggcacataa agcgtcgccc
     1981 tatgtgccgt cctgattcgg aagggattgc acccttccca aataaaatct gattctaccg
     2041 ccccgaagga cggatgtcca agtgggcggg gtttcaaccg aaaaggaaac acgatgaata
     2101 ttcaaacccg ccttgccgca accgccctcg ccctattcct ttccgcctgc ggaaacggcg
     2161 gcgcgcccgc acagcccaaa ggcgaaattt ccgaaaaccg caccgccgcg ttcaaatcca
     2221 tgatgcccga attcacacgc atgggcaaaa tggtcaaaga cgaagaacct tacgatgtcg
     2281 aaaaattcaa acaggctgcc gcggcgtttg ccgaaagcag caagaaaccg ttcacacttt
     2341 ttgagtccga tccgcaaggc aacggccgcg ccctgcccgc cgtttggtcg gatggtgcaa
     2401 aatttgaagc cgaaaaaaca aaattcgccg ccgccgtcga aaaactcaac gccgcagcac
     2461 aaaccggcaa actggatgaa atcaaagccg cctacggcga aaccggcgca agctgcaaat
     2521 cctgccacga cagtttccgc gcgcccgaat aacatccaaa acaaaacaat gccgtctgaa
     2581 gccttcagac ggcattttct ttgccatttc accaagcgca aagcaatcaa aaattatcgt
     2641 taattcaaaa aatacgccat atcctgattt ttgccttact tactccgcca ttttctattt
     2701 ttatttgcta tgcgattaat ctttatcgtc aaatgtcaaa atcgggatgc cggattgggt
     2761 aaacagggca tacaccggct tgagccaata atcttccgga agggaagccg gtttcaatgc
     2821 gtaaacgtgc acattgggaa aatccttcat cgtcaacgcc gcgccgctga aaaaggtata
     2881 aatttcatac ctcgtatgcg ggtttttctt aatctcgcgc aaaatatagt cttcgatgac
     2941 atagggcgaa tttaatgtgg ttacgccgga aagcccgtag gtttggcggg ggtgcggtgc
     3001 gacatattgt atgttgaagt tttttgccgc cttttccgaa atttccttca tctccttgtc
     3061 gggcgaaccc aaaagtatcc gcaccgtgcc gcccgtttcg tcccccgcct tcagttcgga
     3121 cgcatcgaac agcggcaggt aagtcatctt gcggcggccg tcgtccataa tgtttttcaa
     3181 acccttgaat atcgtgtaat gctcgtcgga agcattgcgg gttttggcga tgctccaatc
     3241 tccgatcatc atccgggcaa aattccgctt gatcgtcccg tttacagaaa actcatcgcc
     3301 caaatagctg ctgctttgaa ttaaattgat tgtcccgtcg tcaaaggttt tgatttccgc
     3361 atccgggtaa gtgctcaaaa aggcggcaat gctgactttt tccaaacttg ccaaataaat
     3421 ccgcttgact ttcggcagca gcatcgactt taccttcagc tccgccatcg tcggaatgaa
     3481 attaaacgat ttgttcaaac cgtagggcag gtggaaaaaa tacgcccgct ccgccttatc
     3541 ctttatctga ttgaaataat aatcgtattt ttcattcctg ttttcagaca tcagcaccac
     3601 ataaaaccgc tcccccggat gctgcgccat aatcctttct gccaccttca tctgcaatat
     3661 ggtatagcag aaaatcagat tgaccggttc cccctcttca ttgaagagtt tgtccttcag
     3721 cagggaaacc gcattccttt ccccctgatt tacccggtca aatgtataaa atatcccgaa
     3781 acaaaaaaca atcaggcaca acacggtcaa acaagccttt ttcaagccca tatccctaaa
     3841 actccattcc gacaaattga acatacgccc cgagtgtccc gggacggaaa aatgcggaat
     3901 tttagcagat attccgacag ttaccgaata ttatagtgat ttaaatttaa atcaggacaa
     3961 ggcgacgaag ccgcagacag tacaagcagt acgacaaggc gaggcaacgc tgtactggtt
     4021 taaatttaaa ccactataaa atcaatttca ccgaaaatat gccgccgcac atcaggcaca
     4081 gcacctccga caccgccgcc aaaagcggga aaaccatttc ccccttcctt tcacgtcgcc
     4141 gcgttcaaac acgccaagat cgaacaaagc gcgaacaaag ccgccaaaat atgccataat
     4201 agccacgcac attccccgcg caatgtgttc caaaagaaaa aaccaaccta caagagaata
     4261 atcctatgac tcaaaaatcc accattattt atacccatac cgacgaagcc cccgcgctgg
     4321 cgacccaatc gctgctgccg attgtgcagg cttttgcccg ccacgccgat attgatgtca
     4381 aaactgccga catttccctg tccggccgta ttttggcggc atttcccgaa tatctgaccg
     4441 aagcgcagcg cgtacctgat gcgcttgccg aattgggcga actggtgaaa caacccgatg
     4501 caaacgtaat caaactgccg aacatcagcg catccgtacc gcaactgact gccgcgatta
     4561 aagaattgca gtctaaaggc tttgccgttc ccgactatcc tgccgaccct caaaccgatg
     4621 aagaaaaagc cgtacgcgaa cgctacgacc gcatcaaagg cagcgcggta aaccctgtcc
     4681 tgcgtgaagg caactccgac cgccgcgccc ccaaagcagt gaaaaacttt gccaaaaaaa
     4741 atccgcacag tatgggcgcg tgggcgaaag actccaaaac ccacgttgcc accatgcaaa
     4801 gcggcgactt tttccataac gaacaatccg ttaccgtacc cgaagcgact tccgtatcca
     4861 tcgtgttcac cgacaaacaa ggcaacaaaa aagagctgcg cgcgcctgtt gccctgaaag
     4921 ccggcgaaat catcgacgca accgtgatga gcaaaaaagc cctgctcgcc ttccttgccg
     4981 aacaagtgaa agacgcgaag gcaaaaggcg tattgttctc actgcatatg aaagccacga
     5041 tgatgaaagt gtccgacccg attatcttcg gacacgccgt caaagtgttc ttcgcgcctg
     5101 tatttgaaaa attcggcggc aaactggctg ccgcaggtgt caacgtcaac aacggtttcg
     5161 gcaacctgct tgccaatctg gacaaactgg atgcggacac ccgcgccgcc gtcgaagctg
     5221 aaatcgccgc cgtttacgcc gccaaccctg atttggcgat ggttgattcc gacaaaggca
     5281 tcaccaacct gcacgttccc agcgatgtca tcgtcgacgc ttctatgcct gcgatgattc
     5341 gtaattccgg ccgtatgtgg gacaaaaacg gcaaagcaca agacaccaaa gccgtgattc
     5401 ccgacagcag ctatgccggc gtttaccaag caaccatcga cttctgccgc gaacacggcg
     5461 catttgaccc gacaaccatg ggtactgtgc ccaacgtcgg actgatggcg caagcggcgg
     5521 aagaatacgg ctcgcacaac aaaacctttg aaatcgaagc cgacggtcag gttcaagtag
     5581 ttgatgcggc aggaaaagtc ctgatgcagc acgacgttga agcaggcgac atctggcgta
     5641 tgtgccaaac caaagacgct ccggttaaag actgggtaca actcgccgtc aaccgcgccc
     5701 gcctgagcaa tacgcccgcc gtgttctggc tcgacgaaaa ccgtccgcat gacaagagcc
     5761 tgctcgccaa ggttaaagcc taccttgccg aactggatac caacggcctc gacatccgcg
     5821 tcctcgctcc cgaagaagcc gccaagttca gcctgggccg tctgaaaaac ggcgaagaca
     5881 ccatctccgt aaccggtaat gtcttgcgcg actacctgac cgacttgttc ccgattttgg
     5941 aactcggcac aagcgcgaaa atgctgtcta tcgttccatt gatgaacggc ggcggtatgt
     6001 ttgaaaccgg cgcgggcggt tctgcaccga aacacgtcca acaattcctt gaagaaaacc
     6061 acttgcgttg ggactctttg ggcgaattcc tcgcgctcgc cgtatcgttt gaacatttgg
     6121 cacaaaaaac cggcaacacc aaagcccaag tcctcgccga cactttggat gccgccaccg
     6181 aaaaactgct gctaaacgac aaatcgccca aacgcaaaac cggcgaactc gacaaccgcg
     6241 gcagccattt ctacctcacc ctctactggg cgcaagaatt ggcggcgcaa gacaaagatg
     6301 ccgaattgaa agccgcattt gcgccgttag ccaaatcgtt ggcggaaaac gaagccaaaa
     6361 tcgttgaaga actctctgcc gtacaaggca aagcggtcga catcggcggc tactacgccg
     6421 ccaatcctga aaaagcggca caagcgatgc gcccaagcgc aacctttaat caggcactaa
     6481 gcaccttata agcacaaagg taaaaatgcc gtctgaacat tcgtcgttca gacggcatta
     6541 gttattccta tctgcctgat tatgattaaa cgccgcaccc attggatata aatcatataa
     6601 tctttagttt ttaccctagg aaaagacaat aatatgaaaa ctgttccaac catcctgatt
     6661 agcatccttt tggcaaccgc ccttcctgca tccgcacacg gtatacataa gagcaagccc
     6721 ctgactatgg acgagctgcc gccgatttgc caacaatatt tcaaacgcgc cgaaacctgt
     6781 tacaacaaag ccggcaacaa ggcaaatttt gcacgcaaca acactaaatt cctattccaa
     6841 gccctgcctg ctgccgattt gggacagcgt aagcaaatgt gccaaatcgc tatggattcg
     6901 tttgcagaaa aaacccgcat cagcaatagc aatgccgtct gaaacaatga tttcagacgg
     6961 cattctcaca tcaaaacgca ctagcttgat aaaacccgcc ttccccgcat ccctgacccg
     7021 aacaggatta aacgaagtaa cggctttaac cattatggca agttcccatt ctcaaaaatt
     7081 atag
//
LOCUS       DAXHMX010000111         6752 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHMX010000111 DAXHMX010000000
VERSION     DAXHMX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.111"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="ACFM4S_001625"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="ACFM4S_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6051270.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="ACFM4S_001626"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="ACFM4S_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6051271.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="ACFM4S_001627"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="ACFM4S_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6051272.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="ACFM4S_001628"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="ACFM4S_001628"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6051273.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3332..3408)
                     /locus_tag="ACFM4S_001629"
     tRNA            complement(3332..3408)
                     /locus_tag="ACFM4S_001629"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Val,seq:gac)
     gene            3587..4702
                     /gene="tgt"
                     /locus_tag="ACFM4S_001630"
     CDS             3587..4702
                     /gene="tgt"
                     /locus_tag="ACFM4S_001630"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6051274.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            4712..5794
                     /gene="hemH"
                     /locus_tag="ACFM4S_001631"
     CDS             4712..5794
                     /gene="hemH"
                     /locus_tag="ACFM4S_001631"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6051275.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            5986..6408
                     /locus_tag="ACFM4S_001632"
     CDS             5986..6408
                     /locus_tag="ACFM4S_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6051276.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKKLNRQNAV"
     gene            complement(6451..6741)
                     /locus_tag="ACFM4S_001633"
     CDS             complement(6451..6741)
                     /locus_tag="ACFM4S_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051277.1"
                     /translation="MAKYFTETGIGVREHFDFFGEFVVSPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT         1721 a         1823 c         1576 g         1632 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcgatag aggcgcgttt cagaccattc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcgcggt atgcgtattg gccggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcgata gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacga gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcattt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca ttagggggtt
      721 ttaaaacatc ggtttcaaac acttgaaccc acaatgccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cgaaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     1081 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgcttcttgg cttgttggta
     1141 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcacggcag
     1621 taatccgctt gattttcggt gatattcata atcaccattt gaaccggcgc caaccataac
     1681 gggaatgagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa agaacccaaa
     1741 atggcgcgat gcaacataac aggacgcgca cggtcgttgt tttcagttac atattcggca
     1801 ttcaaacgct ccggcaatac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ctgaacctgc gcgtttttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccga actgtttgta gatgcgaacc aacaattcat tgaacgcacg ggcctcgctg
     2101 acgatttggt cttcggtaca gaaaatatgc gcatcatcct gcacaaatcc acggacccgc
     2161 atcagaccat gcaatgcacc gcttggttca ttgcggtggc aagaaccgaa ctcggccaaa
     2221 cgcatcggca aatcgcgata tgagcgcaaa ccgttattaa aaatttgcac atgacccgga
     2281 cagttcatcg gtttcaccgc gtattcgcgt ttttctgaac tggtcacgaa catattgtct
     2341 ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccataat ttgaggcgtt
     2401 ttgacctctt tataaccggc ggcgttcagc tctttacgca tatgctgttc aatcacttgc
     2461 cacaaagccc agcctttagg atgccaaaac accatgcccg gcgcttcgtc ttgcagatgg
     2521 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg cttttaattc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     2641 atttcattat tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggg ccgcggcaca tatcgacata ttcctgatga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc aatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg ggcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgccgcc
     3181 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tgtcggactg agccgtccgg caaggtaatg ttcaacatca aaaagctcca aaacattaaa
     3301 aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt cgaaccaacg
     3361 acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc aaagaatttt
     3421 tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcgtttctt tatgcaacat
     3481 tgcggcaaga tttggggatg tcgtctgaaa aaccgtggga aatcatttat aatgccgtct
     3541 tttaaaacat atttcgcccc ctgtcgccca actgacattc aatcttatgc ttaaatttac
     3601 cttacacaaa aaagacggat acgcccgacg cggcacgctg gagttgaacc acggcaaaat
     3661 cgaaacgccg gtatttatgc ctgtcggtac ttacggttcg gttaaggcga tgaacccgca
     3721 aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt tgtggctgcg
     3781 tccgggcttg gaggtcgtcg aacagtttgg agggcttcac ggctttatcg gctgggacaa
     3841 accgattttg accgactcgg gcggttttca ggttttttct ttgtcggata tgcgcaagct
     3901 gacggaagaa ggctgtacgt ttaaaagccc gattaacggc gacaagctgt ttttatcgcc
     3961 tgaaatttct atgaaaatcc aaacggtttt gaactcggac atcgcgatgc agttggacga
     4021 atgcacgccg ggcgaggcga cgcgcgaaca ggcgcgaaaa tcgttgcaaa tgagcctgcg
     4081 ctgggcggaa cggagcaaaa aagcctttga agatttgaaa aacccgaacg cactgttcgg
     4141 catcgtgcaa ggcgcgatgt atgaagattt gcgcgaagag tcgctgaaag gcttggaaga
     4201 gctcgatttc cccggcttgg ccatcggcgg cttgtccgtt ggcgaaccta agcccgaaat
     4261 gtaccgcatg ttgcgcgccg tcggccctat cctgccggaa cacaaacctc attatttaat
     4321 gggcgtcggt acgcctgaag acttagtgta cggcgtggca cacggcgtgg atatgttcga
     4381 ctgcgtcatg cctactcgca atgcgcgtaa cggctggctg tttacccgtt ttggcgattt
     4441 gaagatcaaa aacgccaaac acaagctcga caagcgtccg atagacgaaa gctgcacctg
     4501 ctacgcctgc caaaacttca gccgcgccta cctgcaccat ctgcaccgcg ccggcgaaat
     4561 cttgggcgca cagctcaaca ccatccacaa cctgcatttc taccaagtca ttatggcgga
     4621 aatgcgcgat gccatagaac aaggcaaatt tgccgactgg caggcgcgat tccacgaaaa
     4681 ccgtgctcgc ggcacagact gactcccggc aatgccgtct gaagccgctt cagacggcat
     4741 tttgccgctt taccctaccc gcactatttt tcagaaaccg atcatgcttc cattccttcc
     4801 agaaccctcc ctttcctata cgcaacaaaa ccgtaccgcc gttttgctgt tgaacctcgg
     4861 cacgcccgac gcgccgaccg cgcaggcggt acgaccttat ctgaagtcct ttctgaccga
     4921 ccggcgcgtc gtcgaactgc ccaaatggct gtggtatccc attctgcatg gtctggtact
     4981 gacgttgcgc cccaaaaaaa gcgcgcacgc ctatgaaaaa atctggttta aggaaggttc
     5041 gcccctagaa gtttacacag cacgccaagc tgccgcgctt gccgaacgta tgcccgacct
     5101 catcgtccgc cacgccatga cctacggcaa tccttcggtg gcagacgtat tgtcggaact
     5161 gaaggcgcaa ggcgcgggca gactgctggt catcccgatg tacccgcaat atgccgcttc
     5221 cagcagcgga gcggcagtag ataaggtgtg cgaacaatta ctgctgcaac ggaaccaaat
     5281 gagcgtccgc accgtttcgc gcttttatga cgataccggc tacattgatg cgatgaaaaa
     5341 ccacatcctc cgatattggg cggaacacgg acgtgggaaa aaactgatgt tgagttttca
     5401 cggcgtaccg cagaagcact acgacctcgg cgacccctat cccgacgagt gccgccacac
     5461 cgccaaactg cttgccgaag cactggaact gaccgaagac caatatgtcg tatcgttcca
     5521 aagccaattc ggcagggcaa aatgggttac accgagcacg caggatttgt tcggcaaact
     5581 gcccaaacag ggggtaaccg agctggacgt attctgcccg ggctttttgg cagactgttt
     5641 ggaaaccatg gaagaaatcg ccctgatggg gcgggaacag ttttacgaag caggcggcaa
     5701 aagctaccgc tacatcccct gcctcaacga caaccccgac tggatagacg cactcgtcgc
     5761 acttgccgaa gaaaaccttg gcggctggcg ttgaaactgt ttgacccatg tctaaaatgc
     5821 cgtctgaacg ccgtgttcag acggcatttt cccgctactt tagcgtatga tggcatctat
     5881 gccggacaac aacatcaagt gcaaccttac taccgattcg gcaaaaaaat ctttcacccg
     5941 ctacgcaaaa tcaacaacaa ggtttcaaac attaaggaaa caccaatgaa cacaacccga
     6001 ctgccgaccg ccctcgtctt gggctgcctc tgcgccgccg cttctgccgc cgacaacagc
     6061 atcatgacaa aagggcaaaa agtgtacgaa tccaactgcg tcgcctgcca cggcaaaaag
     6121 ggcgaaggcc gcggaaccat gtttccgccg ctctaccgct ccgacttcat catgaaaaaa
     6181 ccgcaggtgc tgctgcacag catggtcaaa ggcatcaacg gtacaatcaa agtcaacggc
     6241 aaaacctaca acggattcat gcccgcaacc gccatcagcg atgcggacat tgccgccgtc
     6301 gccacttata tcatgaacgc ctttgacaac ggcggcggaa gcgttaccga aaaagacgta
     6361 aaacaggcaa aaggcaaaaa aaaactaaac agacaaaatg ccgtctgaaa ccggcaatcc
     6421 ggcttcagac ggcattcaaa tcaaaccttt ctaacgcggt actttcagcc ccatagaccc
     6481 gatattgccg ccaccgtcaa cctccttcgc ggtcagcata aagccgccga tgtcaatacg
     6541 gccgcactcg accggctcct gactgtcgga acgcttatcg aaaagctcgg caaggctcaa
     6601 accctcttcg cccgcttcca gcctcaaacc gtaagcaagt gccaaatcac ccgaacgtgc
     6661 tgccggcgaa acgacaaact cgccgaaaaa atcaaaatgc tcacggacac cgatacccgt
     6721 ctcggtaaag tatttcgcca ttttatagtg aa
//
LOCUS       DAXHMX010000112         6720 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHMX010000112 DAXHMX010000000
VERSION     DAXHMX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.112"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..260
                     /locus_tag="ACFM4S_001634"
     CDS             <1..260
                     /locus_tag="ACFM4S_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6051278.1"
                     /translation="RQLLDGIMRKACRNRPLTETQTKHNRYLSKIRYVVEQSFGTLHR
                     KFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            410..943
                     /locus_tag="ACFM4S_001635"
                     /pseudo
     CDS             410..943
                     /locus_tag="ACFM4S_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
     gene            1005..2300
                     /gene="tyrS"
                     /locus_tag="ACFM4S_001636"
     CDS             1005..2300
                     /gene="tyrS"
                     /locus_tag="ACFM4S_001636"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6051279.1"
                     /translation="MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            2599..3519
                     /gene="ribF"
                     /locus_tag="ACFM4S_001637"
     CDS             2599..3519
                     /gene="ribF"
                     /locus_tag="ACFM4S_001637"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6051280.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFASRTGRVPPCRISPLRTKLELLEGTGCIDAVWVLRFDRHF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIAADMEAAKCW"
     gene            3661..6450
                     /gene="ileS"
                     /locus_tag="ACFM4S_001638"
     CDS             3661..6450
                     /gene="ileS"
                     /locus_tag="ACFM4S_001638"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6051281.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPTGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVAGMRVWEANPVILQWLEETSNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1648 a         1896 c         1764 g         1412 t
ORIGIN      
        1 agcgtcagtt gttagacggc attatgcgca aagcctgccg caaccgtccg ctgacggaaa
       61 cgcaaaccaa acataaccga tatttgtcga agatccgtta tgtggtcgaa caaagcttcg
      121 gtacgctgca ccgcaaattc cgctatgcgc gggcagccta tttcgggctg attaaagtga
      181 gtgcgcaaag ccacctgaag gcgatgtgtt tgaacctgtt gaaagccgcc aacaggctaa
      241 gtgcgcccgc tgccgcctaa aaggcagccc ggatgcctga ttatcgggta tccggggagg
      301 attaaggggg tattggggta aaattaggag gtatttggta cgaaaacagc cgaaatcctg
      361 tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aaggtctcag gctacgcgtc
      421 ccgcgtgttc caaaactggg ccgcctgccg cgccgtttac cgctatgccg aagaacacct
      481 gccccggtat tcaaaagtgg ttttggcgat gcgctggggc agccagatgc ccgaaaacag
      541 ccgctccctt gcctacgatg ccggtttttt ccaaaaattc gaccgtatgc tgcacaaact
      601 ctcatccgaa aaacaagccg tttacctgat ggcggacaac cttgcctcgt cttacaacgt
      661 ccagcgcgcc tatatcttgt cttcacgcat accgggttac cgccaaaccc tgcgcccgga
      721 cgacgaaagc accctgaaag ccaatgcacg catcagggaa ttggcagcca aataccccaa
      781 cgtctatatt attgatgccg ccgcctatat ccccgcagat tttcaaatcg gcggattgcc
      841 ggtttactcg gacacagacc acatcaaccc ttacggcggc acggaattgg caaagcgttt
      901 ttccgaaaaa caacggtttc tcaatacgcg ccataaccat tgattcgctt aaatttgtta
      961 caatcggcgg tttgcaaaaa cgctaatttt tttgaaagag accgatgagc gtcatccaag
     1021 acctgcaatc gcgcgacctt atcgcgcaaa cgaccgacat cgaagcctta gacgctttgt
     1081 tgaacgaaca aaaaatcgcc ctttactgcg gtttcgatcc gaccgccgac agcctgcaca
     1141 ttggacacct gctgcccgta ttggcattgc gccgcttcca acaggcgggg catacgccga
     1201 ttgctttggt gggcggcgcg accggtatga tcggcgaccc cagcttcaaa gccgccgaac
     1261 gcagcttgaa ttccgccgaa actgttgccg gctgggtgga aagtatccgc aaccaattaa
     1321 cccctttctt gagctttgaa ggcggaaatg ccgccattat ggcgaacaat gccgactggt
     1381 tcggcagcat gaactgcctt gacttcctgc gcgacatcgg caagcatttc tccgtcaacg
     1441 ccatgctgaa caaagaatcc gtcaaacagc gcattgaccg cgacggcgca ggcatttcct
     1501 tcaccgagtt cgcctactcc ctgctgcaag gctacgactt cgccgagttg aacaaacgcc
     1561 acggcgcggt tttggaaatc ggcggctccg accaatgggg caacatcacc gccggtatcg
     1621 acctgacccg ccgcctgaat caaaaacaag tgttcggcct gaccctgcct ttggttacca
     1681 aatcagacgg caccaaattc ggcaaaaccg aaggcggcgc agtatggctg aacgcgaaaa
     1741 aaacctcgcc ctatcagttc taccaattct ggctgaaagt cgccgatgcc gatgtgtata
     1801 aattcctgaa atactttacc ttcctgtcca tcgaagaaat cgatgccgtc gaagcgaaag
     1861 acaaggcaag cggcagcaag cccgaagcgc aacgcatcct cgccgaagaa atgacccgcc
     1921 tgattcacgg cgaagaagcc cttgccgccg cgcagcgcat ttccgaaagc ctgtttgccg
     1981 aagaccaaag cagcctgacc gaaagcgact tcgagcagct cgccctcgac ggcctgcctg
     2041 catttgaagt ttcagacggc atcaatgtcg tcgaagcctt ggtcaaaacc ggtttggcat
     2101 cctccaataa agaagcgcgc ggctttgtga acagcaaagc ggttttgctc aacggcaaac
     2161 ctgccgaagc caacaacccc aaccacgccg ccgaacgccc cgacgatgcc tgcctgctga
     2221 ccgacgaaca caaacgtttc ggcaaataca ccatccttcg gcgcggcaaa cgcaatcacg
     2281 cgcttttggt ttggaaataa tccgattgcc gcagaaatgc cgtcattccc gcctgcgcgg
     2341 gaatgacggc ggagggttgt ttgtctcggt ttacctggtt aaaaaagaac gattttcact
     2401 gatgttgcat caggtttggg gcgatgtttc aacacatagc accgcgcctg ctgcgcgctt
     2461 tatgcgtttg gcgcgttcgg ctgcgggaaa tttgcctact tttcccgcgt cgggcgggcg
     2521 taacgggcgg cacactgtct ataaaccgca ataccgttta caatgaccgc ctgtttcacc
     2581 acatacccga acataacaat gagaatcagg ctggggcggc acaacgcgcc cgactttccg
     2641 cacggggccg ccgtaaccat aggcaatttt gacggcgtac acctcggaca caaacacatc
     2701 ctccaaaaac tccgcctcga agccgatgcg cgcggcctgc ccgtcgtagc cgttatcttc
     2761 gagccgcaac ccaaagaatt ttttgcaagc cgcacgggta gagtgccgcc gtgccgcatc
     2821 agccccctgc gtaccaaact ggaattgctg gaagggacgg gctgcatcga tgccgtctgg
     2881 gttttgcgtt tcgaccggca tttttccgaa atatccgcgc aagggtttat cgaccgcctg
     2941 ctgcgtcaaa ctttaaatac gcgctatttg ctcgtcggcg acgatttccg tttcggtgcg
     3001 gggcgggaag gctgttttga actgttggca caacagcccg atatgcagac cgaacgcacg
     3061 ccttccgtca tcgtcgaaga catccgcacc agcagtaccg ccgtccgcca ggccctttca
     3121 gacggcaacc ttgcctatgc gaaaaaactt ttggggcacg actacgtttt ggggggcagg
     3181 gtggtgcacg gcagaaaact cggacgcacc ttaaacgccc cgactgccaa catccgcctg
     3241 cccggccacc gttatgcact cggcggcgtg ttcgtcgtcg aagcggacgg cgcattcggc
     3301 acgcggcgcg gcgtggcgag cttcggcttc aatcccaccg ttgatagcgg ctgttctcaa
     3361 aagcttgaag tccacctgtt cgactttcaa ggcgacctgt acggacaacg gctgaacgtc
     3421 cgcttcctgc acaaactgcg cgatgaggaa aagtttgacg gtatggaaga actgaaaagg
     3481 cagatagcag ccgatatgga agccgcaaag tgttggtagg caaaccttat acaaaccatc
     3541 cgattgggct acaatcaacc ttttaacttt tcagacggca taggggtttc ccgttgtgaa
     3601 atactgtttg aggcgcaatg ccgtctgaaa ccgaaatatt gtaacaatag agatcaaaaa
     3661 atgaccgatt acagcaaaac cgtcaacctg ctcgaaagcc cgtttccgat gcgcggcaat
     3721 cttgccaagc gcgagcctgc atggctgaaa agctggtacg agcaaaaacg ctaccaaaaa
     3781 ctgcgcgaaa tcgccaaagg ccgtccgaaa ttcatcctgc acgacggccc gccgtatgcc
     3841 aacggcgaca tccacatcgg tcacgccgtc aacaaaattc tcaaagacat cattatccgc
     3901 agcaaaaccc aagccggttt tgacgcgcct tatgtgccgg gttgggactg tcacggcctg
     3961 cctatcgaag tgatggtgga aaaactgcac ggcaaagaca tgcctaaagc ccgtttccgc
     4021 gaattgtgcc gcgaatacgc cgccgaacag attgcccgtc agaaaaaaga ctttatccgc
     4081 ttgggcgtgt tgggtgactg ggaccatcct tacctgacca tggatttcaa aacagaagcc
     4141 gataccgtgc gtatgctcgg cgaaatctac aaatcgggct atctctaccg gggcgcgaaa
     4201 ccggttcaat tctgcttgga ctgcggttct tcattggccg aagcggaagt ggaatacaaa
     4261 gacaaagtat cgcctgcgat tgacgttgcc tatccgttta aagacaccgc cgcgcttgcc
     4321 gccgcattcg gtttggcagg tatcgaaggc aaagcgtttg ccgttatttg gacaaccacg
     4381 ccttggacgc tgccggcgag ccaagccgtg tctgccggtg cggacgtggt gtatcaatta
     4441 atcgatacgc ctacaggcaa attggtgctg gcgaaagatt tggcggaaga tgcgctcaaa
     4501 cgttacggct tttcagacgg catcgccatc cttgccgaaa ccaccggcga caagctggaa
     4561 aacctgcaca tgaaccatcc gttcctcgaa cgcgatattc ccatgcttaa cggcgaacac
     4621 gttaccaccg atgccggtac cggcttggtg catactgcgc ccgcgcacgg tttggaagac
     4681 tacgccgtct gcaacaaata cggcatcgag ctttacaatc ctgtcaacgc cgaaggcaaa
     4741 tacatcggcg aaacgccgcg tgtcgccggt atgcgcgttt gggaggcgaa ccccgtcatc
     4801 ctgcaatggt tggaagaaac cagcaacctt ttggcaagca gtaaaatcga acacagctac
     4861 gcccactgct ggcggcacaa aacgccgctg atttaccgcg cgacaggtca atggtttgtc
     4921 ggtatggaca aagccggtgc cgacggcaaa accctgcgcg acaaagccat caaggccgtg
     4981 gacgacaccg aattcttccc gtcttggggt cgcgcgcgtt tggaagccat gattgaaggt
     5041 cgtcctgact gggtggtttc acgccaacgc tattggggca cgccgatgac tttctttgtt
     5101 cacaaagaaa cgggcgagct gcatccgaac tctgccgaac ttttggaaaa agttgccctg
     5161 aaaatcgaag aaaaaggcat cgaagcgtgg ttctccctcg ataagagcga actcttgagc
     5221 gcggaagatt gcgaaaatta cgataaactt tctgacacaa tggacgtatg gttcgattcc
     5281 ggttcgaccc attattccgt tgtgaaacaa cgcgaagaat tggaatggcc ggcggatttg
     5341 tatctcgaag gcagcgacca gcaccgcggc tggtttcaat cgtccatgct gaccggctgc
     5401 gcctcatcaa tgggtcgcgc gccgtataaa cagctgctga cccatggttt cgttgtcgac
     5461 ggcgaaggca aaaaaatgtc gaaatccatc ggcaacgtcg ttgcaccgca agaggtttat
     5521 aacgaattcg gcgcagacat cctgcgcctg tgggcggcat ctaccgatta cagcggcgaa
     5581 ttggcgattt ccaaagaaat cctcaaacgc gtaaccgaaa gctaccgccg tatccgcaat
     5641 accttgagct ttttgtttgc caacttgagc gactttaatc cgattgaaga tgccgtgcaa
     5701 caggcggata tggtggaaat cgaccgctac gccgtggtat tggcacgtca gctgcaagag
     5761 cgtctggcag gcgattacta tccgcgttat gccttccact ttgccgtaaa agacattgtt
     5821 tctttctgct cggaagactt gggtgcgttc tacctcgaca tcctgaaaga ccgcctctac
     5881 accaccaaag ccgacagcca tgcacgccgc agcgcacaaa ctgccctgta tcacatcaca
     5941 cgcagtttgg ttctcttgat tgcaccgatt ttgtgcttca ccggcgaaga agcgtgggac
     6001 atcatcggcg gcggcgaaga agacagcgtc ctcttccata cttggcacga gttcccgacc
     6061 atcaacgaaa aaaccgaagc cgaactggtg aaaaaatgga cggcaatccg cgaagcccgc
     6121 gaagcggtaa ccgccgccat cgagcctttg cgcgccgaca aaaccgtcgg ttcgtccttg
     6181 caagccgaag ccgaaattac cgcgccggaa gaaatggccg gctatctgaa tgctttgggc
     6241 gaagaattgc gctttgcttt gctggtgtct aaagcagaag tgaaagtagg cagcgaactt
     6301 gccgttgccg ctaaagccag tgatggtgaa aaatgcgaac gctgctggca ctacacccgc
     6361 gatgtgggcg cggttgcagg ctatgaaacc gtctgcaaac gctgtgcaga gaatgtcggc
     6421 ggagaaggcg aaacgcgcca ttacgcctga taaagtttga gcaaatgccg tctgaaaccg
     6481 ccgaacagca tttcagacgg cattttttgt gccgcgattt gtctttataa cggcggaggg
     6541 gtttcaagat tgcggtgttg tcggaatgca actgaaccgt cattcccacg aaagtgggaa
     6601 tctagaatct cgaggtttca gtcatttccg atagattccc gccgcgtcgt gggtctggat
     6661 tcccgcctgt gtgggaatga cggatttcga gattgcggtg ttgttggaac gcaactgaac
//
LOCUS       DAXHMX010000113         6717 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHMX010000113 DAXHMX010000000
VERSION     DAXHMX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.113"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            18..1409
                     /locus_tag="ACFM4S_001639"
     CDS             18..1409
                     /locus_tag="ACFM4S_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6051282.1"
                     /translation="MQVFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            1488..2744
                     /locus_tag="ACFM4S_001640"
     CDS             1488..2744
                     /locus_tag="ACFM4S_001640"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6051283.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            2925..3734
                     /gene="zupT"
                     /locus_tag="ACFM4S_001641"
     CDS             2925..3734
                     /gene="zupT"
                     /locus_tag="ACFM4S_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6051284.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            3831..6668
                     /locus_tag="ACFM4S_001642"
     CDS             3831..6668
                     /locus_tag="ACFM4S_001642"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6051285.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     NLDREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFAMPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVATL
                     PESEDAPVAVCNGARLMLKVEIDKATETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
BASE COUNT         1585 a         1819 c         1801 g         1512 t
ORIGIN      
        1 agacaaagga gaaagccatg caagtgtttt tagacaatct caaagccttt ttcgaaaccg
       61 tcagcggctg ggtctgggga tctgttatgt tgatgctgct ggtcggcacc ggcattgtgc
      121 tgaccgttat gctgaaaggc ttgcagttca ctatgttggg ctatgcgctg aaacaggcat
      181 ttgtgccgtc aaagaagtat gaaggcggcg aaggccacga aggcgatatt tcccatttcg
      241 cggcgttgat gaccgcgctg tccgccacca tcggtacggg taacatcgcc ggcgtggcga
      301 ctgcggtggt aaccggcggc ccgggcgcgg tattttggat gtggatgacc gccattttcg
      361 gcatggccac caaatacggc gaaggcgtgt tggcggtgaa ataccgcgtc aacaattcca
      421 aaggcgaaat gtccggcggc ccgatgtatt acatcgaaaa aggcttgggc aaaaactgga
      481 aatggatggc cgtcgcgttt gcgctgttcg gcacattcgc ttccttcggt atcggcagct
      541 cggtgcagtc caactcggtt gcacaggcgg tgcaaaccag cttcggtatc gaacctgcct
      601 ataccggcat tacgttgacc gttctgactg ccgttgtcgt tttaggtggt attaaaggca
      661 tcgccaaagc cgcttctttc atcgtgcctg ctatggcggt gttttatgtg ttgggcggtc
      721 tttccattat cgcgattaat tccgatgcac tgatgcctgc cgtcaagctg attttctccg
      781 atgcgttcag cgcgcaggca gtggcgggcg gcgctatcgg tacggtcatc cgctacggcg
      841 tggcgcgcgg cgtgttctcc aatgaggcgg gtatgggttc tgcgcctatt gccgccgcag
      901 ccgcgaaaac cgaccatccc gtccgtcagg ccttggtttc tatgaccggt acgtttttgg
      961 ataccatcgt cgtttgctcg attaccggca tcgtgttggt aatgggactg ctcggcgcgg
     1021 gcggcgagtt tgtcaaacct gaagtgagcg gcgcggcact gacaaccgtc acattccaaa
     1081 aaatgctgcc cggcatcggc ggctggattg ttaccatcgg cctgattttc tttgcctact
     1141 caaccatttt gggctggtgc tattacggcg aaaaatgcgc ggtgtatgtt ttcggcgaga
     1201 aatttgccgg tttgtaccgc gtgggttatg tttcttccgt tatgctgggt accgtgttga
     1261 gccttgattt ggtgtggctg gcttcggaca cattcaacgg cttgatggca ttgcccaacc
     1321 tgattgcact tttattgatg gcgaaagtca tcgtcaatga aacgcgcgac ttcaaacaaa
     1381 aaatcacaaa cggcgaattg ccgcattaat atccgaggcc gtctgaatcc gaatattcag
     1441 acggcacggc atattaagtc tgttttgaaa aagtcggagg gagaaacatg aaagtgcttg
     1501 ttttaggtgc gggtgttgcc ggcgtgtctt ccgcgtggta tctggcagag gccggacatg
     1561 aagtaacggt catcgaccgc gccaagggtg tggcgatgga aaccagtttt gccaatgcag
     1621 gccagctttc ttacggctat accacgcctt gggctgcacc cggtattccg accaaagcac
     1681 tgaaatggct gtttaaaagc catccgcctt tgctgtttcg ccccgacggc agcctgtatc
     1741 aaatcgaatg gctgtggcgg atgctgcaaa actgcacggc agcgcactat caaaccaata
     1801 aagagcgcat ggtcaggatt tccgaataca gccgtgaaat gttccgccgt tttgaagcgc
     1861 aaaccggcat gaattttgaa gggcgcaaaa aagggacgtt gcagattttc cgtcaggcta
     1921 aagaagttga agctgcagaa caagacattg ccgttttgga acgctacggc gtgccgtacc
     1981 gccgtctgaa gcccgaagaa tgcgcagaat tcgagcctgc gctggcacgc gttaccgcca
     2041 aaattgccgg cggcctgcac ctgcctgcgg atgcaaccgg cgactgccgc ctcttcactg
     2101 aaaacctata caaattgtgt caggaaaagg gcgtacggtt tcatttcaac caaaccatca
     2161 gccgcatcga ccacaacggg ctgcgcatca aaaccgttga aaccgaaaca gggcggtttg
     2221 aagcagatgc cgttgtctgc gcgctcggtt gtttcagcag gacggttttg gcgcagttgg
     2281 atctcaatct gcccatttat cccgtcaaag gctattcctt gaccctgccg gtcacaaatt
     2341 ccgacggcgc accggtgtcc accgttttag atgaaagcta caaagtcgcc atcacgcgct
     2401 ttgacaacag aatccgcgtc ggcggtatgg cggaattgtc gggctacgaa atcaaactgc
     2461 ccgaaaaacg ccgcgaaacc ttggctttgg tcgtcaacga cttgttcccg gaaggcggcg
     2521 atttgagcca agcatcgttc tggagcggcc tcaggccgat gacgcccgac agcacgccgt
     2581 taatcggtcg cacccgcttt gacaacctgt ttctgaatac cggccacggt actttaggct
     2641 ggaccatgtc gctgggttcg gcaaaattaa ccgccgatat cgtgagcggc aaagacaccg
     2701 aaatccgcag cgacgatttg agcctgtcgc gttatcaggc ttaagctgga aagcgataag
     2761 gtttagcggc atttcaaatt taaaggccgt ctgaaaatca tttttcagac ggccttttgt
     2821 ttcaaaatcc gtgtatccaa tcaataaaat aaataataac tattatcata tacattaaaa
     2881 agtgttagtc tgaacctgtc ccagctttta gcgcaggttt tctcatgccc gatttttcga
     2941 tgtccaattt ggccgttgcc ttttccatca cattggctgc cggtttgttt accgtattag
     3001 gcagtggctt ggtgatgttt tccaaaacgc ccaatccgcg tgtgttgtcg tttggtttgg
     3061 cgtttgccgg cggtgcgatg gtatatgttt ccctgacgga gattttcagt aagtccagcg
     3121 aggcgttcgc tgaaatttat gataaagacc acgcgtttgc ggcggcgacc atggcatttt
     3181 tggccgggat gggcggcatt gcgctgattg accgtctggt gccgaacccg catgaaactt
     3241 tagacgcgca agacccgtcg tttcaagaaa gcaaacgccg ccatatcgcg cgagtcggca
     3301 tgatggcggc gtttgcgatt actgcgcaca atttccccga aggcttggcg acgttttttg
     3361 ccacattgga aaatccagca gtcgggatgc ctttggcctt ggcgattgcc atccataata
     3421 ttccggaggg catttccatc gccgcgccgg tttattttgc cacccgcagc cgtaagaaaa
     3481 cggtgtgggc gtgtctgcta tccggcttgg ccgagccgtt gggggcggct ttgggctatt
     3541 tggttttgca gccgtttttg tcgcctgccg tgtttggttc ggtattcggc gtgatagccg
     3601 gtgtgatggt gtttttggcg ttggacgagc tgctgccggc tgccaaacgc tattcagacg
     3661 gccatgaaac cgtttacggc ctgacaatgg gcatggcggt gattgccgtc agcctggtat
     3721 tgttccattt ttaaaacctg tatgccaaag gccgtctgaa atgattcaga cggccttttt
     3781 gcgttataat ccgcacatca aaaataacct taatttcaca aagttccatc atgttagaca
     3841 aatactcccc cgccgaaatc gaatccaagc actatcaaaa ctgggaaagc caaggctatt
     3901 tccaacctga tatggatttg accaaaccgt ctttttccat ccaactgccg ccgcccaatg
     3961 taaccggcac gctgcacatg ggtcatgcct tcaaccaaac cattatggac ggcctgaccc
     4021 gctactaccg tatgaaaggc tgcaacaccg cctggattcc cggcaccgac cacgcgggca
     4081 tcgccacgca aatcgtggtc gagcgtcagc ttgccgcgca aaacgtgtcc cgtcataact
     4141 tggatcgtga aaaattcttg gaaaaagtgt gggaatggaa agaagtttcc ggcggcacga
     4201 ttacgcagca gatgcgccgt gtgggctgtt ctgccgactg gacgcgcgag tatttcacaa
     4261 tggacgatgt acgcgccgaa accgtaaccg aagtgttcgt acgcctgtat gaacagggct
     4321 tgatttatcg cggcaaacgc ttggtgaact gggatccggt attgggtacc gcagtgtctg
     4381 atttggaagt ggaaagcgtg gaagaacaag gctctatgtg gcacatccgc tatccgctgg
     4441 cggacaatcc tgccgaagcc gtcatcgtcg ccaccacccg tcccgaaacg ctgttgggcg
     4501 acgttgccgt tgccgtcaat cccgaagacg aacgttatac ccacttaatc ggcaaagaat
     4561 taatcctgcc gctgaccggc cgcaccattc ccgtgattgc cgacgaatac gttgaaaaag
     4621 acttcggcac cggctgcgtg aaaatcacgc ctgcgcacga cttcaacgac tacgaagtcg
     4681 gcaaacgcca cgatacgcgc ttggttaatg tgttcgactt ggaagccaaa gtattggcaa
     4741 acgccgaagt gttcaacttc aaaggcgaag cgcaaccaag ctttgccatg cctgaaaaat
     4801 acgcaggctt ggaccgcttt gccgcgcgca aacaaatggt tgccgatttg caggaacaag
     4861 gtttcttggt cgaaatcaaa gcgcacacgc tgatgacgcc gaaaggcgac cgaacaggct
     4921 cagtgattga gccgatgctg accagccaat ggtttgtcgc catgtccgct acgccaaacg
     4981 gcggcgagcc tgacagcgaa ttcaaaggct tgagcctcgc cgacaaagcc aaaaaagccg
     5041 tcgacagcgg cgcggtacgc tttatccccg aaaactgggt caacacctac aaccaatgga
     5101 tgaacaacat ccaagactgg tgcatctcgc gccaactgtg gtgggggcat caaatccccg
     5161 cgtggtacga cgaagcaggc aatgtttacg tcgcccgcaa tcaggtggaa gccgaaaaac
     5221 aagccggcaa aacaggcttg acccgcgaag aagacgtatt ggacacttgg ttctcctccg
     5281 cgctcgtgcc gttctccacg ctcggctggc cgtctgaaac cgacgaactc aaagccttcc
     5341 tgccgtccaa cgtcttggtt accggctacg aaatcatctt cttctgggtg gcgcgtatga
     5401 ttatgatgac cacccacttc accggcaaag taccgtttaa agacgtgtat atccacggca
     5461 tcgtgcgcga ccacgaaggc aaaaaaatgt ccaaatccga aggcaacgtc attgaccctg
     5521 tggatttgat cgacggcatc ggcttggaaa aactgctggt aaaacgcacc accggcctgc
     5581 gcaaacccga aaccgcgccg aaagtggaag aagccacgaa aaaactcttc cccgaaggca
     5641 ttccgagtat gggcgcggac gctttgcgct tcactatggc gagctacgcc agcttggggc
     5701 gttcggtcaa cttcgacttc aaacgcgccg aaggctaccg caacttctgc aacaaaatct
     5761 ggaacgccac caacttcgtc ttgatgaaca ccgaaaacca agactgcggt tacggcgcaa
     5821 ccgctgccga accgcgcggc tactccttcc ccgatatgtg gattgtaggc cgtctgaatc
     5881 agaccatcga gcaagtaacg caagcctacg aaacctaccg ctttgatttg gcggcggaaa
     5941 ccctgtacag cttcgtgtgg aacgactatt gcgactggta tctggaactt gccaaagtgc
     6001 agctccaaac cggctgcgcc agccgccaac gcgccacacg ccatacctta ttgcgcgtac
     6061 tcgaagccgc cctgcgcctg ctgcacccga ttatcccgtt catcaccgaa gaactgtggc
     6121 aaaccgtcgc ccctatgtgc gacgcgaaaa ccgccgacag catcatgctc gcccgcttcc
     6181 ccgaagccga cggcggcgat attgttcaga cggcattcgg gcaaatgacc gtgttacagg
     6241 atttgattgg cgcagtccgc aacctgcgcg gcgaaatggg catccagccc aacgtgaaag
     6301 ccccgctgtt tgtcgaaagc gcagacgact tggcggacta cctcaaatac ctgccgatga
     6361 tgacccgact gaccgaagca cagcaagtcg ccaccctgcc cgaaagcgaa gacgcgcccg
     6421 tcgccgtctg caacggcgcg cgcctgatgc tgaaagtcga aatcgacaaa gccaccgaaa
     6481 ccgcccgttt gagcaaagaa gccgaaaagc tgcaaaaagc cttggacaaa ctcaacgcca
     6541 aactctccaa acccggctac accgaaaaag ccccggcgca tttggtggaa aaagacaaag
     6601 ccgatttggc ggaattggaa gacaaaatgg caaaagtgca aactcagttg tcgaagttga
     6661 aagactgatt tgattgaatc aacaaggccg tctgaaaaat gtttttcaga cggcctc
//
LOCUS       DAXHMX010000114         6713 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHMX010000114 DAXHMX010000000
VERSION     DAXHMX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.114"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            173..1231
                     /gene="alr"
                     /locus_tag="ACFM4S_001643"
     CDS             173..1231
                     /gene="alr"
                     /locus_tag="ACFM4S_001643"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6051286.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGITEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1773..3128)
                     /locus_tag="ACFM4S_001644"
     CDS             complement(1773..3128)
                     /locus_tag="ACFM4S_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6051287.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVTTAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETVKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(3143..3415)
                     /locus_tag="ACFM4S_001645"
     CDS             complement(3143..3415)
                     /locus_tag="ACFM4S_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6051288.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3541..4278)
                     /locus_tag="ACFM4S_001646"
     CDS             complement(3541..4278)
                     /locus_tag="ACFM4S_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6051289.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4559..5458)
                     /gene="prmB"
                     /locus_tag="ACFM4S_001647"
     CDS             complement(4559..5458)
                     /gene="prmB"
                     /locus_tag="ACFM4S_001647"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6051290.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5603..6196
                     /locus_tag="ACFM4S_001648"
     CDS             5603..6196
                     /locus_tag="ACFM4S_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051291.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            6308..>6713
                     /locus_tag="ACFM4S_001649"
     CDS             6308..>6713
                     /locus_tag="ACFM4S_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6051292.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANP"
BASE COUNT         1839 a         1666 c         1533 g         1675 t
ORIGIN      
        1 tgggaaagcg attatatcag gaaaagcaaa ccgccttcct acctgaaaac tgctgcttcg
       61 gcttgaagac acaagggtct ttaatatttt aaaagccttg ccgttggatt ataattccct
      121 aattgatttc ttaattttgc taataaacac ttgcttggta aggaatgaat ttatgcgccc
      181 tttgaacgtg cagatcaggt tgggcaacct taggcacaat tatcggattt taaaggaaat
      241 gcacggaggc aagctgttgg cggtagtgaa ggccgacgca tacggacacg gtgcggtcag
      301 atgtgctttc gcgctggcag acttggcaga cggctttgcc gtggcgacaa tcgatgaagg
      361 aatcaggctg cgggagagcg gcattaccca tccgattgtc cttttggaag gcgtatttga
      421 agcatcagag tacgaagcgg tcgaacaata ctcgctctgg ccggcagtcg gaaaccaatg
      481 gcaacttgag gctttgcttt cccgccattg gaaaaaacct gtcaaggtct ggttgaaaat
      541 ggattcggga atgcaccgta ccggtttttt ccctcatgat tacgcttcgg catatgcggc
      601 attgaagcag tcggaatatg tggacagtat tgtcaaattc tcgcatttct cctgtgcgga
      661 cgaacccgaa agcggtataa cggaaataca gatggaagca ttcgatttgg gtactgaagg
      721 gctggaaggc gaagaaagcc ttgccaactc cgccgctatt ttgaatgttc ccgaagcacg
      781 cagggactgg gggcgcgcgg gcttggcgtt gtacggcatt tccccgttcg gaggcagcga
      841 tgacaggctg aaacccgtga tgaggctttc cacccgtatt ttcggcgaac gcgttttaca
      901 gccgcactcc cctatcggtt atggcgcaac attttatacc agcaaatcta cgcgggtcgg
      961 cctgattgcc tgcggttatg cggacggtta tccgcgccgt gccccaagca attcccccgt
     1021 cgccgtcgac ggtaaattga cccgggtcat cggcagggtc tctatggata tgatgaccat
     1081 cgagctggac gcttcacaag aaggtttggg acacgaggtc gaactgtggg gcgatacggt
     1141 caacatcaat accgttgccg aagcggccgg aaccatccct tacgaattga tgtgcaatat
     1201 caaacgtgca aaattcactt atatcgagta accgggttca aacgaaaatg ccgtctgaag
     1261 cctttcagac ggcataaatg cgttttgaca aaatcagtaa aagacattgc caaatagaaa
     1321 atactgataa tgcccacaat ctgaaacccc atccttcccg caaaaacgga aaatcaaaat
     1381 cagaaaccta aaatttcgtc attcccgcgc aggcgggaat ccggtttttt gagtttccgt
     1441 catttctgat aaattctcgt cgtttttcat ttctagattc ccgcctgcgc gggaatgaca
     1501 aaattaaagt ttcagaattt atttgaacaa caaactagat tcccactttc gcggggatga
     1561 cggcggatag gttttgttgt ttcggataaa ttcctgttgt tttttggttt ccagattccc
     1621 acctttaaaa gaatgacgta agaaagcaga aataaggaca cagggatttt ttaaatttgc
     1681 aatattcttt tctaaagaca ttttacccga tgcctataaa caaaaatgcc gtctgaagcc
     1741 tttcagacgg catttcccca tcaaaaccgc aatcagtttt tcatcgattg aaccggtgcg
     1801 ggaatcctgc cgccccggtt gacgaacact tcgcatgagc cttcttttac cggcattaca
     1861 ggcgcgtagc ccaacaggcc gccgaactcg acgctgtcgc cgacggtttt accggttacc
     1921 ggaataatgc gcacggcagt ggttttgctg ttgatcatgc cgatggcggc ttcgtcggca
     1981 atgatgccgg aaatggtgtg cgcgggtgtg tcgccgggaa cggcaatcat atccaagccg
     2041 accgaacaaa cggcggtcat ggcttcgagt ttgtccagcg tcagcacgcc tgcttcggcg
     2101 gcggcaatca taccttcgtc ttcggaaacg gggataaacg cgccactcaa acccccgacc
     2161 gcgctggaag ccatcatgcc gccttttttc acggcatcgt tcagcaatgc caaagctgct
     2221 gttgtgccgt gcgtaccgca gacgctcaag cccatttctt caagaatgcg tgccactgag
     2281 tcgccgacgg cgggggtcgg cgccagcgac aagtcgagaa taccaaacgg gatattcagc
     2341 atttttgagg cttcgcggcc gatgagttcg cccacgcggg taattttgaa agcagttttc
     2401 ttcactactt ccgcaacttc ggtcaatgtc gttgcatctg aattttccaa cgcggctttt
     2461 acgacacctg ggccggatac gccgacattg ataacggcat ccgcttcgcc cgaaccatga
     2521 aacgcgcccg ccataaacgg gttgtcttcc accgcgttgc agaacacgac aattttagcg
     2581 cagccgaaac cttcgggcgt gatttccgcc gtgcgtttga cggtttcgcc cgccagcttg
     2641 accgcatcca tattgatacc ggcacgcgta ctgccgatat tgatggagct gcacacaata
     2701 tcggtagtct tcatcgcttc gggaatggag cggattaaca cctcatccga aggcgacatc
     2761 cctttttgca ccaacgcgga aaaaccgccg ataaaagaca caccgatggc tttggcagct
     2821 ttatccaaag tttgcgccac gctgacgtaa gaatcagcat gggtggccgc cgcgatttgg
     2881 gcaatcggcg taacggaaat gcgctgattc acaatcggta cgccgtattt ggcagacaga
     2941 tattttgccg tagtgaccaa gtctttgccg actgtggtaa ttttattgta aatattttgg
     3001 ttcaacacat tgatatcgct gctgatgcag tcgtgcaaat caatgccgat ggtaatggtg
     3061 cggacatcaa aattctggtc ggcaaccatt ttgacggttt ctaaaatttc gccggattgg
     3121 atactcatca cattcctcca actcaaatgc ggtgcatcgc ttggaagatt tcttcgtttt
     3181 gcatacggat atcaagcgcg agttttttgc tctcttccgc aaacaaatcc aaaacctctt
     3241 gacgcgattt gctgcatttt gaagtgtcca ccaagataat catagtaaaa aaatcgtcca
     3301 tcagctgttg gctgatgttg agaatattga tttggttttc cgccaaaatt ttggaaacat
     3361 cgtacacgat gccgacgcgg tctttaccga tgacggtgat gactgaattg ttcacaggct
     3421 tactccttgc agatatccgt taaagtccga aattatacca ccgttggatt ttgaagaaat
     3481 attgtcaaca atatatacat acaaaatgcc gtctgaaact atttcagaca gcatcaagat
     3541 tcagggttcg attaaataac catccttatc ccactgggtt ttcctgacca acttgtcatc
     3601 ctgataaaca gcttcgctct ttttagaacc atcttcatac cactccaaaa ccaccccgtt
     3661 gcgttgatgg tggcggatag acagttccga gagtaatcgg ccgctttcat cccaagtcag
     3721 aattttggca ggctcatcgt tgaccataac catttccgtc ttgatactgc cgtcggcata
     3781 ccattgcttc catacgccgt ttgccttatt ttgcttaaac tggatttcgc tttccttgcc
     3841 gccgttacgg taatagcggt atcccgtacc ctcactcaag ccatttttat aaggcataac
     3901 ggcagatttt ttaccgttcg gataccagtt gacccactcc ccgtccggct tacccttgct
     3961 gaagcccccc gccatttttt tctgaccatt aaaatgccac aaaatcaaca taccgttttg
     4021 cagggtaggc acaaaagatt tgatttgcgt tgaagcaacg atataaggtt cagaatattt
     4081 cttcatcgac ggataataaa aatcctgcgc gtgcgcaata cccgccacca cactatattg
     4141 cctgatataa gcggcagaag acatcgtcgc cgtcagcttt ccgttctgat taaaataaac
     4201 agaataggtc tgcgccggca aagcggccga aaaacccaac aggacagttg aaaatacaat
     4261 ccgagataat tttttcattg caatagcgat ataaaaacaa ggctgtgttt tagtaatctg
     4321 ttgatttcaa ttatttgcaa gggaaaagac aattattttc cggttaggaa taaacctatt
     4381 ctattgaata tattgaagcc aagtacgcct atcaacacta tattaaaaca ctgccaaaaa
     4441 caattaactt ataaacaata tggtaaggat ttctctgcca agcatcaaac ccgagacaac
     4501 gtatcgtaaa aatgccgtct gaaaacaaat cgtcttcaga cggcatttcc ccttcaactc
     4561 actcttcacc caataactgc tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg
     4621 tttccaacca agtaaaaggc aactccggat acgctgcttc caatacatcc ctgttatgcc
     4681 cgatttccac cagcaataca cctttgggat tcagaaactt tgccgcattc agaagaatct
     4741 gcctggtggc atccaacccg tccgccccgc tgcccaatgc caattccggt tcgtgcaaat
     4801 actcttcagg caataactca accgattccg catccacata aggaggattg gaaacaatca
     4861 aatcataagt gccttccaat ccttcaaaca aatccgtatg aataagccgg atgcgttctt
     4921 ccaaaccata atcttcgaca ttaatccctg ccacttccaa agcatccaag ctcacatcaa
     4981 ccgcatcaat ttgggcatca ggataatgat gcgccatctg aatggcaagg caaccgcttc
     5041 cggtgcaaag atccaaagca ttatgcacca actcatcgta ttctatccaa ggacgaagtc
     5101 cgtcacccaa caattcataa ataaaagaac gaggtatgat tacgcgctca tccacataga
     5161 aatcaaactc tccctgccat gcctggtgtg tcaaataagc ggctggaatg tgttcgacag
     5221 cacgacgctc aataaccgcc agcacttcct ctttttcagc ttccaagagt tttgcatcaa
     5281 gatatggggc aagcatatcc aaaggcaaat tcaaagtatg cagaatcaaa taagctgctt
     5341 catcatgcgc attatctgtt ccatgaccaa aaaagagccc tgcctcatta aaacggctga
     5401 ctgcaaaacg taaaatatcg cggatagtcg tcaattcttg tgctgcctga ttaaacataa
     5461 tatgaaccat tctgcgtata gatactttta attataacag aaacaacaag caaacctttt
     5521 catatcgcca aataaccacc caatctaccc atacaactac ataaatgccc gcgcgaaaac
     5581 catcgcccga acggaaacga caatggccga cggtatgggc aatctgattg gctgggaaaa
     5641 aacggggctt gttgtcggta agcagtggat aaccgcaaaa gacgacaagg tgtccgatgt
     5701 ctgcaatgcc aacggcgaga tgggcgtaat cgggctttac gagcctttct cacacggcgc
     5761 attgacgata cccggtcatc cgaactgccg atgcgaggtt gtttccgtat cgggtggcga
     5821 attgggggaa tttgccgaaa aaaaggagct tcgtaaagcg gctatgcagt atgcgcggga
     5881 taactttatc ggcaaaagct atgtcaataa aaacagcggg catgaactga aggtaacttg
     5941 gcaaggtgtg aaacacgctg cgtcaaaggc aaatcaggcg gaattatcca tcatgacaaa
     6001 acttgatgac ttattgcgct acgcaaaata tgagggttct tattcggata ggaaaggtca
     6061 tcctaatatt attgcagcac ataagtatcg tgccgttgcc aaggttggga atgagtcttt
     6121 aaatatcggt gtgattgtaa gggaatttcc agacgaccat aaacattacg accatttcat
     6181 cttgaaggat gaataaagcc cttttgcagt gtcgttctgg agcggatagc gttaaggcaa
     6241 gtacacttcc agccttgaaa aagggcttta aattcagcat gccatttata caggcaggag
     6301 taaacccatg acaaagttat acgcagaaat cgccaagatg gagacgcagg acgacgacac
     6361 ggtcaaggtt tggggttacg cttcaagcga ggaaatcgat tcggacggcg aagtcatcgc
     6421 ggcggcagct atgaaggcgg cgattcccga ttatatgaag tttggcgcgg ggcgcgagat
     6481 gcacggctca aacgctgcgg gaacggcaat tgaaatcaac gttgaagacg acggcataac
     6541 ctttttcggt gctcatatca tcgaccccgt tgtcgtgtcg aaagtcaaaa caggcgttta
     6601 caaaggcttt tccatcggcg gcagcgttac cgcccgcgat gatttgaaca agtcgcaaat
     6661 cacgggcttg aagctgacgg aaatcagcct tgtcgagcgc cctgccaatc ccg
//
LOCUS       DAXHMX010000115         6709 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHMX010000115 DAXHMX010000000
VERSION     DAXHMX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.115"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(133..1266)
                     /gene="nrdB"
                     /locus_tag="ACFM4S_001650"
     CDS             complement(133..1266)
                     /gene="nrdB"
                     /locus_tag="ACFM4S_001650"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6051293.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1327..2514)
                     /locus_tag="ACFM4S_001651"
     CDS             complement(1327..2514)
                     /locus_tag="ACFM4S_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFC6051294.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(2507..3520)
                     /gene="dcm"
                     /locus_tag="ACFM4S_001652"
     CDS             complement(2507..3520)
                     /gene="dcm"
                     /locus_tag="ACFM4S_001652"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC6051295.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(3578..5857)
                     /gene="nrdA"
                     /locus_tag="ACFM4S_001653"
     CDS             complement(3578..5857)
                     /gene="nrdA"
                     /locus_tag="ACFM4S_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6051296.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTSQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            6386..6697
                     /locus_tag="ACFM4S_001654"
     CDS             6386..6697
                     /locus_tag="ACFM4S_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC6051297.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1795 a         1498 c         1419 g         1997 t
ORIGIN      
        1 gatttttgtt aatccactat aatccactat aaaaggaaca ataaccgatc ctacccgctg
       61 tttttcccat catacaacat acaaatgccg tctgaaacat ccggcttcag acggcatttt
      121 ttcaaaaaac atttacaact caaaatcgcc caaatcatcc gtattcactt cagaatcgat
      181 ctgaccgatc aaataagagg atatttccac ttcctgcggc gcgacctgta cgttgtcgga
      241 cgacagccac gcattaatcc acggaatcgg gttttgattt gcgccttcaa atccggccgg
      301 caaccccacc gcctgcatac gcagattggt aatatattcg acgtattggg ataagatttc
      361 tttgttcaaa ccaatcatcg aaccgtcttt aaacaaatat gccgcccatt ctttttcctg
      421 ttccgccgct tttttgaaga gttggaaaca ttcgtcctgc aactcggcag caatttctgc
      481 catttcagaa tcatcaacac ccgaacgcat cagattaagc atatgctgcg tgccggtcag
      541 gtgcagggct tcgtcgcggg caatcagttt gatgattttg gcgttgcctt ccatcaactc
      601 gcgctcggca aaagcaaacg agcaggcgaa tgaaacgtag aaacggatgg cttccaacac
      661 gttgacgcac atcaggcaga gatagagttt tttcttcaac ccgcgcaaag acacagtaat
      721 gggttttccg ccgacattat gcaccccttc gcccaacagg ttgtaatact gggtgtattc
      781 gattaagtca tcgtaatagc aggcaatgtc ttcggcgcgg gcggtaatgt attcgttttc
      841 gacaatatca tcaaacacga ccgacggatc attcacaata ttgcggatga tgtgggtata
      901 gctgcgcgag tgtatggttt cgctgaagct ccacgtttca atccacgttt ccaactcggg
      961 aatcgaaacc aaaggcagca aggcaacatt cggactgcgc ccttggatgg aatcgagcag
     1021 tgtttgatat ttcagattgc tgatgaaaat atgtttttcg tgttcaggca ggttggcgta
     1081 gtcgatacgg tcgcgcgaca cgtcgatttc ttccggccgc caaaaaaacg acaattgttt
     1141 ttcaatcagt ttttcaaata cctcgtattt ctgctggtca taacgggcga cattaaccgg
     1201 ctgaccgaaa aacatcggct cattcagcgc gtcgtttttg gttttgggaa aggtgctgta
     1261 tgacatagtt aagtgttcgc aggacataat ctattctcta tattaattaa aaatgatctg
     1321 taagttttaa ctaacctgct gagactttat atatttaata aaattttcta aagttaaaac
     1381 atgggttttc ccaaaagttc caaatgtttt tacggattct ttagggacaa ctaaggttaa
     1441 attctcatca ctcatttcct gaatttgagt atcagatata ccttcttgta gagtaaataa
     1501 atgcttatga ggaattcgat ctgcttcatt taaaacttgg cgccagcgat ccttacaagt
     1561 agtctttgaa cctagcattg tcagcttttc agtttgaaat atgaaattcc ctgattcatc
     1621 catcgcatgg tactcttcac ttccaggaaa taaaaaatct ggtttctttt tcccttctgt
     1681 cttcgcttgc gtctcaaatt ttaaggaaaa ttcgtcaaat attcttgata aatgtaactc
     1741 taaagatttt cctgctcttg cttttctgcg atttgtaaaa gaatgtgcaa aattaatgaa
     1801 gctatccaaa tcagtaattt ttgatttaat aatttcaaat tcacgttttt caaaaatgga
     1861 aaataattca tattcctttg aaacccattg ttttaacaca ttactaacgt tagttttatt
     1921 taatgcaata ttttcccttg ccaaaactgc catttcttca gttttgggga agttaggaaa
     1981 tagtgaaaat aattttgtta ggttatcctc tacttcttgt tttttaggta aatataaact
     2041 tcctggtaaa atgttagttt ctgcgataaa tatttcaata tcttcatctg aagataaaac
     2101 aaaagcatgg aataataaat ctttataaca agctctgcac aataccaata aggaaccact
     2161 atatttatca cttaaaaatt caaaattctt gccaaaaccc gtaattctcg cctcatttct
     2221 cgtgccttgg ccgtaatatt taaaattgct attagtgacg cttccatctt gccaattaat
     2281 tttgattgaa atagttttat ttgttccctt ttggcaaact acatcaaaga ggtcgtctga
     2341 aaaatgtttt tgaatataaa atcctgattg gtgactacca gtagtaccaa catcattggg
     2401 acgaatataa cgacagtata ctgcaattga tttatttgct gtctgtattg ctgaagctac
     2461 taagtcatcg gatttattta ctacttgtat tgtctgagct gccaagtcat tcatttttta
     2521 actcatttat aatttgttta gcaatagctt gaaccaacgg tacagcaatt gaattgccaa
     2581 actgcttgta tgcagctgtc ttggatactg catcaataac aaaatcttta ggaaatccca
     2641 ttaaacgcga gcactcccta ggtgtcagct tcctaggatt ttttcctttc tgagggatga
     2701 gtatttcgga accatctttg taatatcgtg cagatagagt tcgtgatatt ccatctaaat
     2761 caactaatcc aaaaccaaat ccattaccct ttgccttatg ttttttagcg taattttgaa
     2821 ggtaaagcca taagttatca gaaagagtaa aagaattatc tacatcatct tccaaaattt
     2881 gctttaattt tggttgtgat tctggtgggg aaggaaaatt gaaatttatt tccttattaa
     2941 aatattgtct atcaaaacct acaataaaaa tacgctccct attttgagga acataatatt
     3001 ttgcattcat aacttgataa aatatctgat agtcaagctc ttctaaagtc cctttaatta
     3061 ctttaaatgt atttcctttg tcatgcgaaa caaggttttt cacattctct aaaagaaaaa
     3121 ttttaggtcg atgttttcca ataatttcag caacatcaaa aaatagagtt ccctgcgcct
     3181 tatctaagaa gcctgtttct cgtcctaggc tttttttctt tgaaacacca gctatagaga
     3241 atggctgaca cgggaatcct gctgttaata catcaaactt acttggaata gctgctttgg
     3301 tttcctttaa tgtaatatct ccataaggaa tatcattaaa atttacttgg taggtttgac
     3361 gggctttatc atcccattca ctagaaaata cacatcgccc accaacattc tccattgcaa
     3421 tgcgaaaacc acctattccc gcaaataaat caataaaagt aaatttctca ttatttaagg
     3481 taactatatt atctaattga ttttgtattt tatttttcat attttatatt tcagatgatt
     3541 tattaatcta aaggccatct gaaaactccc cctttcatca aatcttacaa gccccgcccg
     3601 cgcagccgtc atcctgaata tcggtctgcg tatcgtccgc gccgtcgcgg gtgttatggt
     3661 agtacagggt tttgacgccg tatttgtagg cggtcagcag gtctttgagc atttgtttca
     3721 tagaaacttt gccgccttcg aatttgcccg ggtcgtaggc ggtattggcg gaaatcgatt
     3781 gatcgacgaa tttttgcatc acgccgacaa gtttcaggta gccttcgttg ccgggaagct
     3841 gccacagggt ttcataggca tttttcaggg tttcaaactc cggcacgact tgtttcaaaa
     3901 tgccgtcttt cgatgctttg accgttacca atccgcgcgg cggctcgatg ccgttggtgg
     3961 cgttggcgat ttgcgaactg gtttcagacg gcatgagcgc ggtcagggtg gagttacgca
     4021 ggccgtattt gacgatgtcg gcacgcaggc tttcccagtc gtaatgcaaa ggctcgccgc
     4081 agacggcatc caaatctttt ttgtaagtgt cgatgggcag tttgccttgc gaataaacgg
     4141 tttggttaaa gagcgtgcac gcaccgtatt ctttggcaag gtttgccgat gctttgagca
     4201 ggtaatactg tatggcttca aaggtacggt gggtcagacc gagcgcggag ccgtcgctgt
     4261 agcggacacc gtttttcgcc agataataag catagttaat cacgccgatg ccgagcgaac
     4321 ggcgacccat agtagaggta cgcgcggctt ctaccggata tccctgataa tctaaaagtg
     4381 catcgagcgc acgcacggtc aagtcggcaa gcccttccaa ttcgtccaag ctgtttaatg
     4441 cgcccaagtt aaaggcagac agtgtacaca gggcgatttc gccgttcgga tcgttgatat
     4501 tgtccagcgg tttggtcggc agggcgattt ccatacacaa gttggactga tgaacaggcg
     4561 cgacgcacgg atcgaacggg ctgtgcgtat tgcagtgatc gacgttttga atgtagatgc
     4621 gcccggtccc ggcacgctcc tgcatcagcg tggaaaacag gtcggcagcc ggaatgatac
     4681 gcttgcgtat gttcgggtct tgctcgtatt tcgtatagag ccgctcaaat tcgtcttggt
     4741 cggcaaaaaa cgcttcgtac aaccccggaa cctcgttggg cgaaaacagc gtaatgttgc
     4801 cgcccttaat caggcgggtg tacagcaggc ggttgatttg cacgccgtaa tcaagctgac
     4861 ggatacggtt gtcttccaca ccgcggttgt ttttcaacac cagcaggctt tcggcttcga
     4921 tatgccacaa ggggtagaac aaagttgccg ctccgccgcg cacgccgcct tgcgaacagg
     4981 atttgaccgc cgcctgaaac attttgaaaa acggaatgca gccggtatgc cgcgcttcgc
     5041 cgccccggat ttcgctgtcc aaaccgcgga tacgtccggc attgatgccg atgcccgcac
     5101 gctgggaaac gtatttcaca atcgcgctgg tggtggcgtt gatggaatcc aggctgtcgt
     5161 cgcattcgat cagtacgcag cttgagaact ggcgcgtagg cgtacgcacg ccgctcataa
     5221 tcggggtcgg cagcgatact ttaaatgtgg aaacggcatc gtaaaaccgt ttgacgtaat
     5281 cgaggcgtgt ttctttggga tatttgctga aaagacacat cgccaccaga acatataaga
     5341 actgcggtgt ttcataaatc tggctggtaa cgcggttctg taccagatat ttgccttcga
     5401 gctgtttgac agcggcatag gaaaaggaca tatcgcgttc gtggtcgata taggcgttca
     5461 gttcatcaaa ttcttcgcgg ctgtaatcct caaggatatg cctgtcgtat tttccggcat
     5521 cggtgagctt tttaacgtgg tcgtaaaggt gcggcggctc gtattcgccg taggctattt
     5581 tacgcagatg gaaaatcgcc aaacgcgcgg caaggtattg gtagtccgga gtatcttccg
     5641 aaattaaatc ggcagcggcc ttgatgatgg tttcgtggat gtcgtcggtg cggatgccgt
     5701 tgtagaactg gatgtgcgat ttcaactcga cctgcgacac ggaaacattt tccaatcctt
     5761 ccgccgccca agtaacgaca cggtgaatct tatccaaatc aatggcttct aatcttccgt
     5821 ctcgtttggt tactttcaaa tcagtcggtg tattcatcgc ttcctcttcc actcttgata
     5881 ttcaagacac ggtcttttca aacaaattaa gacagacaat atagtggatt tttggcattt
     5941 tctccagtct tgacaacggt tgcatttttc agtttcaggc aatgcgcgat aaaacccgcc
     6001 tgtcgtattt ttcctataat atttgtttta tctgataatt ctttaccgat aaaaaacggg
     6061 taaatttttt gccttttgac cggctccggc tacaaggcgg tgaaataagg attttccgac
     6121 gaaaaaggaa agcttccggt tttctgcccc tgcgaattgt taaattttgc aaagtatgat
     6181 tttgccgcgc cgccgccgac aaattccatt ttcttaccga ttgaaattta ttattgagat
     6241 taatgtgtta tttgaatccg catatcaaac ggcaagtttt gtcggctgaa tgagtctgaa
     6301 actgccgaca atttgcccgt tccatccttc catcctatac tggaaggaat gacaaactga
     6361 aaggatcgtc atatctgtca aaaagatgct tgccatactg ttgtctgcaa tattgggact
     6421 ggtatcgaca actgccgctg ccggtacgtc agaacccgcc caccgccata ccaaacatat
     6481 ccgcaaggca aacaagcaga tgctgcaccc cgaatgcagg aaatatttgg aacgccgtgc
     6541 cgcgtggtac cgatcgcaag gcaacgtgca ggaattgcgc gaaaacaaaa aggcgcgcaa
     6601 agcattccgc tccctgcctt atgcggaaca gaaaatccaa tgccgggcgg cttatgaggc
     6661 tttcgatgat ttcgacggcg gcagtttccg ccgttaatcc catataaaa
//
LOCUS       DAXHMX010000116         6634 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHMX010000116 DAXHMX010000000
VERSION     DAXHMX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.116"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            102..1007
                     /gene="glyQ"
                     /locus_tag="ACFM4S_001655"
     CDS             102..1007
                     /gene="glyQ"
                     /locus_tag="ACFM4S_001655"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6051298.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1084..1494
                     /locus_tag="ACFM4S_001656"
     CDS             1084..1494
                     /locus_tag="ACFM4S_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC6051299.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFGRFMDELIAYLVVNGFEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     DREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            1515..3578
                     /gene="glyS"
                     /locus_tag="ACFM4S_001657"
     CDS             1515..3578
                     /gene="glyS"
                     /locus_tag="ACFM4S_001657"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6051300.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLIAGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINAAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAAQMREQGKVVA
                     SFAERKAAIQTALEGQARRLNATVAADEALLDEVTALVEYPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMADDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3595..4683
                     /locus_tag="ACFM4S_001658"
     CDS             3595..4683
                     /locus_tag="ACFM4S_001658"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6051301.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGKMRRLFTM
                     RQYFSILHRLIKNRRQARSDSAGKEQEI"
     gene            4683..5510
                     /locus_tag="ACFM4S_001659"
     CDS             4683..5510
                     /locus_tag="ACFM4S_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6051302.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5522..5617
                     /locus_tag="ACFM4S_001660"
                     /pseudo
     CDS             5522..5617
                     /locus_tag="ACFM4S_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            5659..6501
                     /locus_tag="ACFM4S_001661"
     CDS             5659..6501
                     /locus_tag="ACFM4S_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6051303.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLA
                     EDGWLKERFDPDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1701 a         1769 c         1750 g         1414 t
ORIGIN      
        1 atgtatgccg tgttttcaaa tcagtctgtc ttcgagataa acctatccaa acagcaatgg
       61 aaacagtaaa ataaaccctt tattttatcg aatcataagc catgcttacc ttccaacaaa
      121 tcatcttcaa actgcaaaca ttctgggcag acaaaggctg caccgtcatc caacccttcg
      181 acatggaagt cggtgcaggt acttcccatc ccgccacctg cctgcgcgcg ctcgggcccg
      241 agccttggtt tgccgcctac gtccaaccca gccgccgccc caaagacggc cgctacggcg
      301 acaaccccaa ccgcctgcaa cactattacc aattccaagt cgccctcaaa cccgcccccg
      361 ccaatatcca agacctttat ctcgactccc tgcgcgaatt gggcatcgac cccaaagtgc
      421 acgacatccg ctttgtcgaa gacgactggg aaaaccccac cctcggtgcg tggggtttgg
      481 gctgggaagt ctggctcaac ggtatggaag taacccagtt cacctatttc caacaagtcg
      541 gcggcatcga ctgcaccccc gtactcggcg aaatcaccta cggcatcgaa cgcttggcga
      601 tgtacctgca aggcgtggaa aacgtctatg acctcgtttg ggcaaaaacg ctggacggta
      661 atacagtaac ttacggcgac gtgtaccacc aaaacgaagt cgaacaatcc acctacaact
      721 tcgaatacag cgatgccgat tggctgctgc gccagttcaa cgactacgaa gcgcaagcca
      781 aacgcctgct tgccgaagaa aacgccgccc tcgccttgcc tgcctacgag ctggttctca
      841 aagcggggca tacgttcaat cttttagacg cacgcggcgc gatttccgta accgagcggg
      901 caacttatat tggacgtatt cgtgcattga gccgcgccgt ggcgcaaaaa tatgttgaaa
      961 gccgcgaaaa actgggcttc cctttaatga aggcaaatgc tgcctaaggg ggcgacgatt
     1021 ttttacgttc gggcggcacg ataaggcagc cgtccgtaaa ccattatata ttttaggaaa
     1081 atcatgcctg tctatttccg caccccaagc aggaaacgcc tgcacaaaat gaaccgccgc
     1141 cagcataaaa agctgcattt gggcgagttt caaacattga ttttcggcgt tcaaggcagt
     1201 ttgcaccaag ctgaggacga tgccgaagcg ttcggccgct ttatggacga gctgattgcg
     1261 tatcttgtgg taaacggctt tgaaatggcg ggcgggggcg attgcggcga tttttcgtgc
     1321 ctgtttcaaa aatccggtgc ccgaagcttg ggagaaaccg agaaaaaagc cattatctct
     1381 tggctggcag accgcgaaga tgttgccact ttgtacgcca ccccgctggt tgacggtttt
     1441 tatggggatg acggctggga aaattacagc gaaacctata aacaggcaga ttaaccaaat
     1501 tcaaagaaag ccgaatgatg acccaaaccc ttttaatcga actccttacc gaagaacttc
     1561 ccccaaaagc cctgaataat ttaggcaacc atttcgccgc ttccgttgcc gaaggcttgg
     1621 aaaaagcgca actgatagcc ggcgcggcag aatttaccgc ctacgcttct ccgcgccgtt
     1681 tggcggttca agtcaaaaac gtcaaagccg tgcaagccga tcagaaaatc gtgaaaaaag
     1741 gccctgccgt ggcgaatgcc gtaaaagacg gtgcgccgac caaggctttg gaaggtttcg
     1801 cacgcggtgc gggcgcgaaa atcgaagact tgaccatcgt ccacgacggc aagcaggacg
     1861 tgtacgccta cgaatacgtc caaaccggca aaccgttggg cggacttttg gaagacatta
     1921 tcaatgccgc agtgaaaaaa ctgccgattc cgaaagtgat gcgttggggc agcagcacat
     1981 ttaccttcgt gcgccccgtc cacgggctga ttgtgctgca cggcggcgac atcgtaaacg
     2041 tcagcgtttt gggtctgcaa agcagcaatc aaaccttggg acaccgcttc ttgtccaacg
     2101 gcgaaatcac cattgaaaac gccgacagct acgccgcaca aatgcgcgag caaggcaaag
     2161 tcgtcgcttc gtttgccgag cgcaaagccg cgattcagac ggcattggaa gggcaggcgc
     2221 gccgtctgaa cgcgaccgtt gccgccgatg aagcattgct ggacgaagta accgcgctgg
     2281 tcgaataccc cgttgttttg gaagccggtt ttgaggaaca cttcctcgcc gtgccgcagg
     2341 aatgcctgat tctgacgatg cagcaaaacc aaaaatactt cccgctgctc gaccaaaacg
     2401 gcaagctgat gaaccgcttc ctgctggttt ccaacctgca aaccgaagat ccgtcgcaca
     2461 tcatccgagg caacgaacgc gtcttgcgcg cgcgcctgtc tgatgccgag ttcttctaca
     2521 aacaagacca aaaagcgact ctggaaagcc gcctgcccaa gctggcaaac gtggtgtacc
     2581 acaacaaaat cggttcgcaa gccgaacgca tcgaacgttt gcaaagcatt gccgcccaca
     2641 tcgctaaagc gttgggcgca gatgctgccg cagccgagcg cgccgcgcgt cttgccaaag
     2701 ccgatttggt taccgaaatg gtcggcgagt tccccgaatt gcaaggcacg atgggcaaat
     2761 actatgcccg cttggacggc gaaactgaag aaatcgccga agccgtcgag caacattacc
     2821 aaccgcgttt tgccggcgac aagctgcccg aaagcaaaat tgccactgcc gttgcacttg
     2881 ccgacaagct ggaaaccctg gtcggcattt ggggcatcgg tctgattccg accggcgaca
     2941 aagaccccta cgccctgcgc cgcgctgcct tgggtatttt gcgtatgctg atgcagtatg
     3001 gtttggacgt gaacgaactg attcagacgg cattcgacag cttccccaaa ggtttgctca
     3061 acgaaaaaac gccgtctgaa accgccgact ttatgcaggc gcgccttgcc gtgttgctgc
     3121 aaaacgatta tccgcaagac atcgttgccg ccgtattggc gaaacagccg cgccgtttgg
     3181 acgacgtagt ggcgaaactg caagccgttg ccgcgttcaa acaactgccc gaagccgccg
     3241 cgctcgccgc cgccaacaaa cgcgtgcaaa atctgctgaa aaaagccgat gccgagttgg
     3301 gcgcggttaa cgaaagcctg ctgcaacagg acgaagaaaa agccctcttt gccgccgcgc
     3361 aaggtttgca gccgaaaatc gccgccgccg ttgccgaagg caatttccaa accgccttgt
     3421 ccgaactggc ttccgtcaag ccgcaagtcg atgccttctt tgacggcgtg atggtgatgg
     3481 cggacgatgc cgccgtgaaa caaaaccgtt tgaatctgtt gaaccgcttg gcagaacaaa
     3541 tgaacgcagt tgccgacatc gcgcttttgg gcgagtaacc gttgtacagt ccaaatgccg
     3601 tctgaagcct tcagacggca tcgtgcctat cgggagaata aattgcagcc tttagtcagc
     3661 gtattgattt gcgcctacaa cgtagaaaaa tatttcgccc aatcattagc cgccgtcgtg
     3721 aatcagactt ggcgcaactt ggagattttg attgtcgatg acggctcgac agacggtacg
     3781 cttgccattg ccaaggattt tcaaaagcgg gacagccgta tcaaaatcct tgcacaagct
     3841 caaaattccg gcctgattcc ctctttaaac atcgggctgg acgaattggc aaagtcagga
     3901 atgggggaat atattgcacg caccgatgcc gacgatattg ccgcccccga ctggattgag
     3961 aaaatcgtgg gcgagatgga aaaagaccgc agcatcatcg cgatgggcgc gtggctggaa
     4021 gttttgtcgg aagaaaagga cggcaaccgg ctggcgcggc atcacaggca cggcaaaatt
     4081 tggaaaaagc cgacccgaca cgaagatatt gccgactttt tccctttcgg caaccccata
     4141 cacaacaaca cgatgattat gaggcgcagc gtcattgacg gcggtttgcg ttacaacacc
     4201 gagcgggatt gggcggaaga ttaccaattt tggtacgatg tcagcaaatt gggcaggctg
     4261 gcttattatc ccgaagcctt ggtcaaatac cgccttcacg ccaatcaggt ttcatccaaa
     4321 tacagcatcc gccaacacga aatcgcgcaa ggcatccaaa aaaccgccag aaacgatttt
     4381 ttgcagtcta tgggttttaa aacccggttc gacagccttg aataccgcca aataaaagca
     4441 gtagcgtatg aattgctgga gaaacatttg ccggaagaag attttgaacg cgcccgccgg
     4501 tttttgtacc aatgcttcaa acggacggac acgctgcccg ccggcgcgtg gctggatttc
     4561 gcggcagacg gcaagatgag gcggctgttt accatgaggc aatacttcag cattttgcac
     4621 cggctgatta aaaaccgccg gcaggcgcgg tcggattcgg cagggaaaga acaggagatt
     4681 taatgcaaaa ccacgttatc agcttagctt ccgccgcaga acgcagggcg cacattgccg
     4741 caaccttcgg cgcgcgcggc atcccgtttc agtttttcga cgcactgatg ccgtctgaaa
     4801 ggctggaaca ggcaatggcg gaactcgtcc ccggcttgtc ggcgcacccc tatttgagcg
     4861 gagtggaaaa agcctgcttt atgagccacg ccgtattgtg gaaacaggca ttggacgaag
     4921 gtctgccgta tatcgccgta tttgaggacg acgttttact cggcgaaggc gcggagaaat
     4981 tccttgccga agacgcttgg ctaaaagaac gctttgaccc cgattccgcc tttatcgtcc
     5041 gcttggaaac gatgtttatg cacgtcctga cctcgccctc cggtgtggcg gactactgcg
     5101 ggcgtgcctt tccgctgttg gaaagcgaac actgggggac ggcgggctat atcatttccc
     5161 gaaaagcgat gcggtttttc ctggacaggt ttgccgccct gccgcccgaa gggctgcacc
     5221 ccgtcgatct gatgatgttc agcgattttt tcgacaggga aggagtgccg gtttgccagc
     5281 tcaatcccgc cttgtgcgcc caagagctgc attatgccaa gtttcacgac caaaacagcg
     5341 cattgggcag cctgatcgaa cacgaccgcc tcctgaaccg caaacagcaa tggcgcgatt
     5401 cccccgccaa cacattcaaa cgccgcctga tccgcgcctt gaccaaaatc agcagggaaa
     5461 gggaaaaacg ccggcaaagg cgcgaacagt tcattgtgcc tttccaataa aaggaggaga
     5521 gatggacatc gtatttgcag cagacggcag gatgcggcgg ctgtttacct tgaggcaata
     5581 cttcggcatt ttgcgccgat tgctgaaaaa ccgttgaaaa acgccgcttt atccaacaga
     5641 caaaaaacag gataaattat gcaaaaccat gttatcagct tagcttccgc cgcagaacgc
     5701 agggcgcaca ttgccgatac cttcggcagg cacgacattc cgtttcagtt tttcgacgca
     5761 ctgatgccgt ctgaagaact gaatcggatg atggcggaat tggtgccggg attggcaaaa
     5821 cagcacctgt tgagcgaagt ggaaaaagcc tgctttatga gccacgtcgt attgtggaaa
     5881 caggcattgg acgaaggcgt accgtatgtt gccgtatttg aggatgatgt cttactcggc
     5941 gaaggggcgg agaaattcct tgccgaagac ggctggctaa aagagcggtt tgacccggat
     6001 tccgccttta tcgtccgttt ggaaacgatg tttgcgaaag ttattgtcag accggataaa
     6061 gtcctgaatt atgagaaccg gtcatttcct ttgctggaga gcgaacattg ggggacggct
     6121 ggctatatca tttcgcgtga ggcgatgcgg tttttcttgg aaaggtttgc cgttttgccg
     6181 gcagagtgga ttaaagcggt agattggatg atgtttactt atttctttga taaggagggg
     6241 atgcctgttt atcaggttaa tcccgcctta tgtacccaag aattgcatta tgccaagttc
     6301 ctcagtaaaa acagtatgtt gggtagcgat ttggaaaaag atagggaaca agaaagaaga
     6361 caccgccgtt cgttgaaggt aatgtttgac ttgaagcgtg ctttgggtaa attcggtaga
     6421 gaaaagaaga aaagaatgga gcgtcaaagg caggcggagc ttgagaaagc ttacggcagg
     6481 cgggtcatat cgttcaaata gtttgtgtaa aatatagcgg attaaaatta gaaatggaca
     6541 cactgtcatt cccgcgcagg cgggaatcca gtccgttgag tttcagttat ttccgataaa
     6601 tcaccgcaac gttaaatttc tagattcccg cttt
//
LOCUS       DAXHMX010000117         6479 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHMX010000117 DAXHMX010000000
VERSION     DAXHMX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.117"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..334
                     /locus_tag="ACFM4S_001662"
     CDS             <1..334
                     /locus_tag="ACFM4S_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6051304.1"
                     /translation="DASGLGEKIKDGKKTVLSCEEEQKICNTFTNKQAVEDFSVVIGY
                     DEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDR
                     LAMLKFNS"
     gene            366..482
                     /locus_tag="ACFM4S_001663"
     CDS             366..482
                     /locus_tag="ACFM4S_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6051305.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(642..1979)
                     /gene="ribBA"
                     /locus_tag="ACFM4S_001664"
     CDS             complement(642..1979)
                     /gene="ribBA"
                     /locus_tag="ACFM4S_001664"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6051306.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGAALLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            2088..2222
                     /locus_tag="ACFM4S_001665"
     CDS             2088..2222
                     /locus_tag="ACFM4S_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051307.1"
                     /translation="MPSEAGFVASDGIAAILAGRGLFVPTSRALLIWGLPEAYSEKNF
                     "
     gene            complement(2201..2857)
                     /locus_tag="ACFM4S_001666"
     CDS             complement(2201..2857)
                     /locus_tag="ACFM4S_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFC6051308.1"
                     /translation="MILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANAWTK
                     IVRREIIIKNNLFFPKGYIHEDFPYSLKLARFVKTFAFYDNPFYQYRVLGNSISHNVK
                     YKNFSDMLAHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNIILIY
                     RKYFSFKEKCRKIFGAKTIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFFSE
                     "
     gene            complement(2854..3216)
                     /locus_tag="ACFM4S_001667"
     CDS             complement(2854..3216)
                     /locus_tag="ACFM4S_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6051309.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGGGFSLIYNNLQTKRLI"
     gene            complement(3372..3965)
                     /gene="ribA"
                     /locus_tag="ACFM4S_001668"
     CDS             complement(3372..3965)
                     /gene="ribA"
                     /locus_tag="ACFM4S_001668"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6051310.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3958..4632)
                     /locus_tag="ACFM4S_001669"
     CDS             complement(3958..4632)
                     /locus_tag="ACFM4S_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051311.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRTAYLTAQTVMDMGTTSIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            4786..4870
                     /locus_tag="ACFM4S_001670"
     tRNA            4786..4870
                     /locus_tag="ACFM4S_001670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4820..4822,aa:Leu,seq:tag)
     gene            5390..6424
                     /gene="purM"
                     /locus_tag="ACFM4S_001671"
     CDS             5390..6424
                     /gene="purM"
                     /locus_tag="ACFM4S_001671"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6051312.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDMVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1612 a         1573 c         1687 g         1607 t
ORIGIN      
        1 cgacgcatcg ggcttgggcg aaaaaattaa agacggcaaa aaaaccgtac tttcctgcga
       61 agaagaacaa aaaatctgca atactttcac gaacaaacag gcagtggaag atttcagtgt
      121 ggtaatcggc tacgatgaaa tcaaagcgaa gaatcacagc ttgtcggcgg ggcagtattt
      181 cgaggtaaag attgattatg tggatatttc cgccgacgaa tttgcgcaaa aaatggcggg
      241 attttcagcg gatttggata aacttttcgc cgaatctgcc gaattggaaa aagagattaa
      301 ggatagattg gcgatgttga aattcaattc ataaataccg atgaatgaaa ccggtcaaac
      361 ttaaaatgcc gtctgaaagc ctttcagacg gcattttgtt tggtttggaa aatacttatg
      421 ccgttctttc cctgaaagag agaatccaaa aaaccaaagc cacaggaatt tatcggaaat
      481 gacaaaaacc cgacgaaccg gattcccgcc tgcgcgggaa tgacgaatta gaagttaccc
      541 gaaatttgca aaaacaaaat ccaacccaac agaccgggtt ttcgtttgca cggaaataat
      601 gcaataaata aagcaaatat aaagtatttg aatttactat attatttttc cgcttcttca
      661 aagccgacga cttccaaacc gaagccggtc aggccggtga aagatgaggg ctgcccgagg
      721 acgcgcagtt ttttgacgtt gaggccggcg aggatttgcg caccgatgcc gtagcttttg
      781 ctgtcccatt tgtaggcttg gtttgcacct ttgggcaggg tgcggtcgag cagggcggcg
      841 ccgtcttcgg tgcggtgcag aaggatgact acgccgcttt cggcctgttg gatgcgctcg
      901 agtgctttgg gcagcgacca tgaatgacgc gggttagctt ggatgaagtc catgacgctg
      961 aagggttcgt ggacgcggac gagggtttcg gtgtcggcgg agggcgtgcc tttgacgagg
     1021 gcgaggtggg tttcgccgga gagtttgtcg acgtaaacgt gttgttggaa ctcgccccac
     1081 ggggtttgta caggcgcatt gcccatgtct tcaagcaggc tttcggtacg gctgcggtat
     1141 tcgatgaggt cggcaatcgt gccgattttg agcttgtgtt cttcggcgaa tttcatcagt
     1201 tcgggcatac gcgccatcgt gccgtcgtcg ttgatgattt cgcaaataac ggaggcagga
     1261 atcagcccgt tcatttgcgc caggtcgacg ccggcttcgg tgtgtccggc gcggacgagt
     1321 acgccgcctt tttgggcgcg aagcggaaag atatgaccgg gttggacgat gtcttcgggt
     1381 ttagcggtcg gggaaacggc ggtttgaata gtcagggcgc ggtcggcggc ggaaatgccg
     1441 gtggtaatgc cgtgtgcggc ttcgatggag acagtaaagt tggtgccgta ttgcgcgccg
     1501 tttttttggg tcatcatcgg cagcccgagt ttttcgacca tttcgccgtc catcggcagg
     1561 cagaccaagc cgcgcgcgtg tttgatcatg aagttgatgg cttcgggcgt gacgaattgc
     1621 gccgccatca gcaggtcgcc ttcgttttct cggtcttcgg catcggtgat gatgaccatt
     1681 ttgccggctt tgatgtcggc taggatttcg gggatggggg aaatatggga catggtgtgt
     1741 tttcctgtgt gttcgggcgg gcgggcggtg tgttcggtat cgagccagaa ggcgggcatt
     1801 tttgccgcct gttcgatttt acgggctttt ttctcgccga aggatttgtt tttcaaaagg
     1861 gcggagagtt cgcctgtgtt gagggcaacg gcttcggcaa aacgggtttg caaaccgccg
     1921 tagtgttctg ctatccattg ccgcaggttg cggcggcgca gaccggctgt gtcggtcatc
     1981 gtttcatcct ttgtagaaat cggatgtagc ggattgtaat cgcgtattta ccaaaaagca
     2041 aatctgtttt tttcgccaaa ccgaattatt tgctttttgg taaacagatg ccgtctgaag
     2101 ccggattcgt ggcttcagac ggcattgccg caatccttgc cggccggggg ctgtttgtcc
     2161 caacttcccg cgcacttctg atttggggtt tgccggaagc ttattccgaa aaaaatttct
     2221 gataaacggc cttgattgcc gggtacagca taggcggtac gagcaggcgc aatatcggca
     2281 atcctatgat gaatgcggtc ttcccgataa aaaccggacg gattgtcttc gcgccgaata
     2341 tctttctgca tttttcttta aatgaaaaat atttccggta gatgaggata atgtttttgg
     2401 aaaaataaag ccttaccaat atagacctca gatagccgat attgtcgaag acaaattttt
     2461 gcaaaccgcc gtagatgggg gaatttttgt tttcgactaa aaaatccaca ccccggtcga
     2521 gatgcgccag catatcgctg aaatttttgt atttgacgtt gtggctgata gagttgccga
     2581 gaacgcggta ttgataaaaa gggttatcgt aaaaggcaaa agtcttgaca aaacgcgcca
     2641 atttcaaact gtacgggaaa tcctcgtgaa tgtatccttt tgggaaaaac agattgtttt
     2701 tgatgattat ttcccgcctg acgatttttg tccacgcgtt ggcgatatag taccgcccct
     2761 ccaccaacgt ttcaaaatgg cggacaaaat cattatccga aaagtccgcc cctttgggga
     2821 tgacgtggta attgagggac gagggatgca ggatcaaatc aacctttttg tctgcaagtt
     2881 gttgtaaatc aaagagaatc cccccccccc cgcgtttttt gaacggttgg tatcggccca
     2941 ataatcgtcg ctgtccaaaa agattaggta atcgcctttt gccgcccgga taccggcgtt
     3001 gcgggcatcc gacagcccgc cgttttcttg atgaatcact tttatatgcg gatatttgcc
     3061 tgcatattcg tcgcaaatct tcccgcagcc gtccggcgaa ccgtcatcga ccaaaatcat
     3121 ttcataatcg gcaaaatttt cggcaagcac ggaatccacg cagcagcgga ggtatttttc
     3181 cacattgtaa atagggacga tgatggagaa aatcataaat atcaatccat tatattaaga
     3241 tgtttgcgcg tatgcctcaa acccgcgctc gcaatgcgtt tgcatccact ataaaactta
     3301 ttgctttatc aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg cccgttccct
     3361 gcaactttgc cttattccga catcagatgt cccagcttgt ctgctttggt ttggaggtag
     3421 cgtttgtttt caaggttttc cccgacgtgc agggggatgc gttcgaccac gttaatcccc
     3481 gcatctttca gggtttggat tttttcgggg ttgttggtca gcagtttgac cgagcggatg
     3541 cccagatatt cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac gggcagcccg
     3601 agtgccaaat tggcttcaac cgtatccata ccttggtctt gcagatgata ggcgcggatt
     3661 ttgttaatca gcccgatgcc gcgtccttcc tgacgcagat agacgatgat gccgcgcccc
     3721 tctgtctgta cggccctcat ggccgcttca agttgaggtc cgcagtcgca ttttctcgag
     3781 aacagcgcat cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg attgccgtct
     3841 gaaaaatcac cgacggtcag cgcgacgtgt tcctgcccat ttgcctcttc aaagccgtgc
     3901 atcgtaaata cgccccattc ggtcggcagg cggcaggaag caacatagtt taataattca
     3961 gacattttca tctccttctg cggcagtgat gcccaaaagc tgcttcaaag gttcggacaa
     4021 agccaaggca agagccaccc attccacctg tgctgccgta cccgcacact ctgcgcattc
     4081 aaattccaca tgaaccacgc ccaacacacc gccactttcc gtgcagaccg gaatggaaat
     4141 ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctct cccaaatcca accaacggcg
     4201 tacatccgag gcaacattca tccagccgct ttgcgccgaa cggcaagcca gcgaaacctt
     4261 gcctgccgct tcatccaaat cccacaggtt ttccaaaacc tcgccctgcc gggacaggca
     4321 aaccagccga aaggcggcat tttcagacgg catcagggca taaacggctg cggcgcgcac
     4381 gcctttacag cgtgaaaaca ccgaatccaa agccatgaaa agccgtttca gaccttcctc
     4441 attcccgctg ttttgctcca aataatcggc aagcctccaa tcctcatgct caagccacaa
     4501 aaccgaacgc tcaatcgaag tcgtccccat atccattacc gtctgcgccg tcagatacgc
     4561 cgtccgaacc tcgtcaagcg gcagcttcaa accctgagtc agcagaaaat ccttaatcaa
     4621 agcagcaggc atacccaaat tccgtcaata aaaacaaaaa accgcccgat tccgatgtca
     4681 ggcggacgaa aaacagattt taccgcccca aaccccaagc catcaacagc cccgccgaaa
     4741 accttgcaca aaaaaacaaa atctgtataa ttgtccaatt cccatgcgga cgtggcgaaa
     4801 ttggtagacg caccagattt aggttctggc gccgagaggt gtgagagttc gagtctctcc
     4861 gtccgcacca ataatgataa ataaatcata tagttaatgc ttatcgggac aaattagtgc
     4921 cacaaaacat aagtcgtctg cttttcagcg ggcggctttt ttatctgcct atatgtttca
     4981 acacgcagaa cggtacataa agtgccgcca tttgttctac cccgaatcag aagcgggtgc
     5041 ttccgcccgc aatcagattc accgccccta atgccggggt ttcaactgga aagaagaaaa
     5101 tattgatttt gatgatggca tcaaaaaacc ccttccgccc gtctgccaaa aggaatggtt
     5161 aagaaaagca atgcgtcttt tgatggaacg cgatatataa ggaattgagg ggaagggcag
     5221 gcgggtttct gcccgttaat cctgcggacg atcccgccaa acccgacacg cgcgggcata
     5281 tggcggattg tttacaatat tgtctaaaaa tctgaacctg caaacgtgaa ataaggtaga
     5341 atacgccccg ttattttacc gtccaatccc catttaccaa ggaaaaacga tgagtacttc
     5401 attgagttac cgcgatgcag gtgtcgatat cgacgcaggc gaccaactgg tcgaaaacat
     5461 caaaccgttt gccaaacgca ccatgcgtcc agaagtattg ggggatttgg gcggttttgg
     5521 cgcattggtc gaaatcggca agaaatataa aaatcccgta ttggtcagcg gtacggacgg
     5581 tgtgggtacc aagctcaagc ttgcctttga ttgggataaa cacgatatgg tgggcatcga
     5641 ccttgttgca atgagtgtca acgatatttt ggttcaagga gcagaacctc tgtttttctt
     5701 ggattatttc gcctgcggca aattggatgt tccgcgcgca accgatgtca tcaaaggcat
     5761 tgcacaaggt tgcgaagaat ccggctgcgc cctgattggc ggggaaactg ccgaaatgcc
     5821 gggcatgtat cccgtcggag aatacgattt ggcgggtttt gccgtcggcg tagtggaaaa
     5881 agagaatgtc attaccggcc gcagtatcgg cgcgggcgat gtggtattgg gtttggcttc
     5941 caacggcgca cattcaaacg gctattccct tatccgtaaa atcatcgaac gcgacaatcc
     6001 cgatttggat gccgagtttg ataatggcaa aaccttgcgg gaggctgtta ttgcgccgac
     6061 ccgtctgtat gtgaaaccga ttcttgccgc tttggaaaaa tttaccatta aaggtatggc
     6121 acacattacc ggcggcggca ttaccgaaaa cgtgccgcgc gtgttgcctg aaaacacggt
     6181 tgcacaaatc gatgctaaat cgtgggaatt gcccaagctc ttccaatggc ttcaaaaggc
     6241 gggcaatgtg gaaacccaag aaatgtaccg aacctttaac tgcggcatcg gcatggtcgt
     6301 tattgttgct gccgaagatg ccgatgcggt tcagggtctc ttgggtgaac aaggtgaaac
     6361 agtttaccgt ttaggtttga tccgtgagcg tcagggagac gagcatcaaa cccaggttgc
     6421 ctgattgctt ctatagcgaa atgccgtctc aaacatgggt ttggacggca tttttatag
//
LOCUS       DAXHMX010000118         6389 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHMX010000118 DAXHMX010000000
VERSION     DAXHMX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.118"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            33..335
                     /locus_tag="ACFM4S_001672"
     CDS             33..335
                     /locus_tag="ACFM4S_001672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051313.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(417..2504)
                     /locus_tag="ACFM4S_001673"
     CDS             complement(417..2504)
                     /locus_tag="ACFM4S_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC6051314.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2507..3772)
                     /locus_tag="ACFM4S_001674"
     CDS             complement(2507..3772)
                     /locus_tag="ACFM4S_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6051315.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3816..3953)
                     /locus_tag="ACFM4S_001675"
     CDS             complement(3816..3953)
                     /locus_tag="ACFM4S_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051316.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     I"
     gene            complement(4993..5229)
                     /locus_tag="ACFM4S_001676"
     CDS             complement(4993..5229)
                     /locus_tag="ACFM4S_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6051317.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            complement(6283..6389)
                     /locus_tag="ACFM4S_001677"
                     /pseudo
     CDS             complement(6283..6389)
                     /locus_tag="ACFM4S_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117427.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2045 a         1123 c         1105 g         2116 t
ORIGIN      
        1 ttatctagga cagcccctta aaaaatatcc ctttgaaaga ttttgatgaa gtttgtgaaa
       61 agtgggagaa atcctttaaa gaaaatggaa aagaaattga atataatcaa aaatattcaa
      121 gaatttatgg tgaagttcgt ggagctggct ttaatggcga tatcggagaa aagctacatc
      181 gttgtgccgt gatggaatgg gctgaaaatg agaccgtgaa attagcccaa aaatgggaac
      241 aagagcagaa aaaacaacaa attcaacaga aaaaggaaac tgaaaaatcg ccaaaacaca
      301 aagccagtcg tgatgattgg gaaatggaac gttaaaccgc tattttcatg tattctatta
      361 aattatttga aaaaagactt cactttttgt taaagaaaaa tgaagtcttg ttctgttcaa
      421 cctaaattca gaattttatc tgccgattga attgtttctt ggcggtgtgc aaccattatt
      481 ttcataatac caagactttt taggttatgg ttaatttttt gttcattttc tacatctaaa
      541 tggctacttg cttcgtctaa aaatagaatt ttgggtcgtt tatacaatgc acgagccaag
      601 ataactctct gcttttgtcc acctgataag atatttccca tatcgccaat caaggtctca
      661 tagcccattg gcattttaag tatatcgtca tgtatttgtg ccatttttgc acattgttca
      721 atgagctcca tatttgggct ttcatcaaaa aatgaaatat tttccccaat agaacctgca
      781 aaaaggacat catcttggcg aacaatcccg ctcaatcccc taataaagga tggagaaact
      841 tgatatatat catgcccatt aatactaact gtaccagttt caggttttag gctacctgtt
      901 aaaatgttta acaaagtgga cttcccccga ccagattgtc ctgttaaaac aactgcttca
      961 ttatctttaa attccaaatt aatgttttca aaaagatatg gctcattatc agcatatctg
     1021 aatgagacgt tttcaacttt aagaaccagt tgttcattat ctaatttagg tatatgatta
     1081 tacttaataa tttcagtttc tgtttcattt aaagtaatgt cagccaaacg ttcagcatga
     1141 agccctaaca ttttgatttg gatgtattgg tcaacgagag aagctgttct gctttcaaat
     1201 tgccctttat aagccaaaaa agccatcaga acaccgactg taaatgaacc atctaaaatt
     1261 gcgcttgcac caagataaat tatgataata ttttccatgc taaacaacag tttatttgaa
     1321 aattcaaata aagcagagag tttatctgtt gtcagcttgg tattgactgt attcacaaat
     1381 aggctcatcc aagtgccatg tctttgataa tgtttatcaa ataatttaac tgattggata
     1441 ccacgaatgg tttccatgaa atatgagttt tgtttggctt catgaacaat attttcttct
     1501 gttgcatttc ttaatgggta atatgcaagc caacgaatta gtatgtacaa aacaagtgtt
     1561 aaaagaacaa tcagcgataa ttgagtgctg taaattgtca ttaacacgaa agtaaaaaca
     1621 gccattaagc tatttaaaac taaaacaaaa aaagtagaag ttagtgtttc ttggatatga
     1681 tctattgaac caaatcttga aatcacatct cctaaatgtc gtttactgaa atagtcatta
     1741 ggtaagtcaa gtaacctttt aaatatattg gctttccatt gtaaattaag agttgtagat
     1801 aggtgcatac ctacccatgc ttgtaacagg ctaattaact gttgcaggat agccagtaaa
     1861 ccaaatccca aagtaagggt cagtaataaa tttttatcag cagttacaat gacatggtct
     1921 attacccatt gcataaagaa tggactaacc aatgcaaaga cttccaaaga aatagctaat
     1981 ataagcattt gaattaaaga gcgttttaag cctgaaatcc cccttaatag agataatatt
     2041 ttgattttct ttgtttcttt tttctcttca aaatgggtat tggggaataa ttctagggca
     2101 atccctgtga atttttgtga aacttcgtcc attttgattt ttcgcatacc gacagcaggg
     2161 tccataatga cgatactgtc tttggaaatg gaacaaagta caacaaaatg gtttaagttc
     2221 caatggagaa tgcagggtag ttgtaaattt gacagctcat ctaactctaa acgcaaagct
     2281 cgtggcgtta aattcatttc attgccaaat ctcatgatgt ctgcaagatt tgcgcccttt
     2341 aatgacaggg tgtatttttg gcgtaacgtt cttaaatcag tgtgaaagcc ataataggac
     2401 aagatggatg tcaggcatgc taaaccacat tcagcaactt ctgtttgcag aatgacaggt
     2461 agctttttgt taaatccaaa ggacagtctt gataaataat ccatttttaa ttgatttttc
     2521 ctgaaatgct gtaaagtggg tcaagtaccc attcgtacaa tttttttcgt tcatggagaa
     2581 tatctgcttc taaaatcatg ccaatttgaa gcggcttgtt ttctccgtat gctttaatcg
     2641 tttgtttttc caatttaact ttcacaagat aggcaggttc atttaataag gttgggttag
     2701 tgaaaatgat acctaaacct gatagctctt gtttaccgag agcagttctg gcaactgaaa
     2761 taatttctcc tgtggcatgt ccaaattttt ggtaagggta cgcttggtaa cgtaaaacaa
     2821 ctttatcttt cggtttaata aaaccaacag ctttactggg tatgtaaaga ttggcgacca
     2881 attcagtttg ttcagggaca atgcttaaca gcaatttaga cggttcaact tgttgcccta
     2941 tatcaacatt aattgttgat atataacctg atttactagc tcgtatggtt tgttcggatt
     3001 tcaaatcaaa atccaaaatt tcttggttca tttccgtaat cgcacggttg agttggctca
     3061 attcggtttt atggcgttca ggcaggctgc ttaatgtaat tttctgttca tcaagttccc
     3121 tgattgcatt attttgttca agttttaggc tctccaaacg tgagcgttgt tccaataaat
     3181 ggctttcggc ggtcatctta tcttgttggg atactgcgcc ttgactggct aaaaacttgt
     3241 tcttgttaag ggttttttcc gctaaacgaa tttgacgatt ttgccctgta atttgctgtt
     3301 taatattctc taattgattg tttaaacgat gaatgttgtt atgaacattt ttttgctcat
     3361 tttgatgaat gcgctttaaa cgttccaatt cttgtaatgc caaagttttt ttaaggttgg
     3421 cttctgctgc caatttggct tgtacgtttc ctttttcgcc aaaacgcgat gtggaaagtt
     3481 tgaacaatgg ttcgccagct ttgacaaagt taccatcttc aacaaattta tgcgtaatcg
     3541 tgccgatatc ggaagagtaa acacgaacca cccccatagt tggaagtaat tgaccttcaa
     3601 cggttgtttt attggtatag ctaccaaaaa tcaaaaagat aatgatacac agagcaatga
     3661 gaaaagcgca aaaagtcaga aataaaaaag agaatggacg ggtcaagata acctgacctg
     3721 tccacttatt ttgttgggct acaaagactt cttttcggaa taagtgggac atttattatt
     3781 atttcttaaa aaaatggata gaaaaacacc aataattaaa tacttatgcc taataacata
     3841 aatttgctat tgattgatgt ttctatttta attacattaa atttaaccaa taatccagaa
     3901 atggttgcag ctaaaatgca aaagacagtg gcgtaaatgg tttcagtatt cataattttt
     3961 ctctttcaaa attgttcaat ctaaatagaa aaaggtaact tcaatacata ttgctgtaac
     4021 tgaaattacc ttttccttaa tgatgtaatt atcttatggc ttcatcatgc cacgaccgat
     4081 attgtatccg ccagcagcag agaaaatacc agctccaatt gcgccaaccc agcctactgg
     4141 tcctgtccac ttgttccgtt gagctacaaa gacttctggt ctgaaaaatg agtgattgtt
     4201 agacatgtgt acttatttat gtttctttga ttgggataaa tttgtaaaaa caaaaaaaag
     4261 gcaagttgtt attatagaag aacttaaaat gcttaacata ctcattgagg tttgtaccac
     4321 ataatgagat acgagataat ttgttgggag taaaatcaaa aaaagattaa atatttaaaa
     4381 taatcactat attttttcat tatttgttcc tttttgtatt taagttctta tgaaaaacta
     4441 gggtattttg aatagaaaat atcaaaatac cctagtttta ttaaggaata ttaccaccaa
     4501 caagtgcttc catattgtac agcaccagtg attgcaccac caatagctcc gaatttcgca
     4561 cttggaatag caccaatacc acctgcaaat gaaccaacaa ttgccccacc agctgctcca
     4621 cctaatgcac taccaatggt attttttgag aaatcacgcc agttacaagc agcaccagaa
     4681 acttgttaaa tttcattcaa agtcaaaact tgcataatat tcacctttgt taagtgtggt
     4741 tgagaaaaaa gtaaattttc atcgttactc attatttgca atcatgcata tactgtaacc
     4801 aagcactata ttcagttgga tcatagtttt tcctactggg ttgtctgata tgactattgg
     4861 cactattggc acaattggga ttagcaactt tattgtgagt acctaatgga ttgatatttc
     4921 ctgtaaagtg gctattgcat gcgcttaatg agcatagatt tattaatgtc agaaaggata
     4981 aaacaaaaaa tcttaccatt ttttactaat accaaaagta ggtttcgaaa actgtacacc
     5041 aagaccttgt acatatccaa attgcttgct aacagaaaac tctactccac tattgttagg
     5101 ggaataggaa gcaccaattt taccaacagc aaccatatct tttttaccta ggttgccgcc
     5161 accctgtgca aagatatgga atttgccacc tgacacttca actaattcag aggtgtgtaa
     5221 ctctttcatg attttctcct gtaaagaagt atggttggaa atgtaaatca gaattaacct
     5281 ttcttaagtt taatttaata ctttttttgc aagtatttta ttttaaatta aatcaatatt
     5341 ttaaaaatat ttacttgtat actgaatatt tacttgtata ctgaaaaaga aactcctaat
     5401 ggtggagtgg ggcttttgtt taagtaagca ttgaaaaaat ttaaatttcc cattatgatt
     5461 aacttaatgc aagagctttt tcaggacgta caaaccagcg aacgggcgtt ctgatgtggg
     5521 caggttggtt gtcgctaacc aatttcagta gctcaatcgt cagattggta tccggcccta
     5581 tcaagctaag gagaggctgc ggctactaca caaagcacct gaaattattg ggtgcttttg
     5641 tctttttatg gcattgtacc aatttgtgta ccacgagggc ttgttcgacc aaacgcggta
     5701 acaactgaat acacactttc cccctcaact tctttgcgtt ctaaaacgac tgtcccaatc
     5761 cgagaataga caatcatagg acgatgattg ccccttacat cactaaattc actaaaaatt
     5821 tttgcgcctg attgaaaaat catttccaca taaaagggtt acacctgttt catcgtggct
     5881 taatccaaac cgcgttaaat ctgctttgtg ttctgccaaa atgtgaatca caccaaagcc
     5941 gtgattaacc tttttgtgtt cgccgtgttt taaaacaatt tgacctgccg ggaagtctgc
     6001 tctattgttc ggacaatagg gggaatatac caattacttt aactacatca ttttttgaca
     6061 tttttcttgc ctttcaattt aaaatccaaa atcatactgc cataatttag caatccaaaa
     6121 aaatttaggc agcagtcatg gtttcgtatt ttagaaaacg aaacttggtt tggtgttttg
     6181 aagcatttca gtacacaaac atggaatttt aaattgtctt gtgttgcctt gatttaggat
     6241 gttccagttt tttaataaat agtttggggc tgtcctagat aactaggaca aacttgattt
     6301 tactaattgt tttaaaatgg aacaagaact tttatctcac tgttgttaaa acgtcgttcg
     6361 cactccttta aatacagctc aaaatgcgc
//
LOCUS       DAXHMX010000119         6048 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHMX010000119 DAXHMX010000000
VERSION     DAXHMX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.119"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..207
                     /locus_tag="ACFM4S_001678"
     CDS             <1..207
                     /locus_tag="ACFM4S_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051318.1"
                     /translation="FSELYMFQQRTAHQAPHCVLPERDYPTIRFYRYKQTGFNRKGMG
                     IKSISDISRAMPSENQSPLSDGIV"
     gene            284..1231
                     /locus_tag="ACFM4S_001679"
     CDS             284..1231
                     /locus_tag="ACFM4S_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6051319.1"
                     /translation="MNILSKISSFIGKTFSLWVALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1384..2508)
                     /gene="rluD"
                     /locus_tag="ACFM4S_001680"
     CDS             complement(1384..2508)
                     /gene="rluD"
                     /locus_tag="ACFM4S_001680"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6051320.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2507..3310
                     /locus_tag="ACFM4S_001681"
     CDS             2507..3310
                     /locus_tag="ACFM4S_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6051321.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3368..5587)
                     /locus_tag="ACFM4S_001682"
     CDS             complement(3368..5587)
                     /locus_tag="ACFM4S_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6051322.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1497 a         1891 c         1446 g         1214 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagagg acggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actaccctac gattagattc tatcgctata aacagacggg tttcaaccga
      121 aaaggaatgg ggataaagtc catttccgac atctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggtcatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt attatgaata tcctcagtaa
      301 aatcagcagc tttatcggaa aaacattttc cctctgggtc gcgctctttg ccgccgccgc
      361 ttttttcgcg cccgacacct tcaaatgggc ggggccttat attccttggc tgttgggcat
      421 tattatgttc ggtatgggtt tgacgctcaa accttccgac ttcgatattt tgttcaaaca
      481 tcccaaagcc gtcatcatcg gcgtaatcgc acaattcgcc attatgccgg caaccgcctg
      541 gctgctgtcc aaactgttga acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg
      601 ctgctgcccg ggcggtacgg cttccaatgt gatgacctat ctggcgcgcg gcaatgtggc
      661 tttgtcggtt gccgttacgt ctgtttccac cctgatttcc ccattgctga ctcccgccat
      721 cttcctgatg cttgccggtg aaatgctgga aatccaagcg gccggtatgt tgatgtccat
      781 cgtcaaaatg gttttgctcc ccattgtttt gggtttgatt gtccataagg ttttgggcag
      841 taaaaccgaa aagctgaccg atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat
      901 tatcggcgcg gttgttgggg caagcaaagg caagattatg gaaagcggcc tgctgatttt
      961 tgcggttgtc gtactccaca acggcatcgg ctacctgctc ggcttctttg ccgccaaatg
     1021 gaccggcctg ccttatgatg cacaaaaaac gctggccatc gaagtcggta tgcaaaactc
     1081 gggtctggcc gccgcgctgg ccgccgcaca ctttgccgct gcgccggtcg ttgccgttcc
     1141 gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg ctgctggcaa cttattgggc
     1201 ggccaaagcc ggtaaacata aaaaacccta aacgcctttc atcataatgc cgtctgaacg
     1261 gggcaaacaa aactggcggt ttctgcccgg ccccttcaga cggcctttgt ttgaaatcgg
     1321 cggggacgat tgcccggctg ctccggttcg gctgcctgcg ttcgccccgc ctttcaagcc
     1381 acctcactcc cttacataaa ccacttcgac atcgtagtcg tcttcgttcc aatcgtcatc
     1441 gtcgtccgcg ccgaatttgt cctgccattc ctcttcattg ctcaaagacg aatccaaacc
     1501 ggcttcaaga cggaggacag acaataaatg atatatgtcg tccggaatcg gtgcctcaaa
     1561 cgaaacggtt tcgccgcttt ccggatgggt aaaactcaag cggtaggcgt gcaatgcctg
     1621 acgcgcaccc aaacttttaa cggcttcttt caccgtgtcg ccgcacggat ggcgcgggtt
     1681 gccgtaaacc gggtcgccgg caagcggatg gttggcctca cgcatatgga cgcggatttg
     1741 gtgcgtcctg cctgtttcga gcgagcattc gatatagctg tgggcaagat agcgttccaa
     1801 cactttgacg tgggtaacgg ctggtttgcc gccgaatttg acgactgcca ttttcaggcg
     1861 gttgtgcgga tcgcgtccga tttgggtttc gattttaccg tcaaagggga cgatgccgtt
     1921 ggcgacggcg cggtagatgc gtttgaccgt gcgttcttga agctgcctca cgagggaatt
     1981 ttgcgccgac agggttttgg caaccaccat cagcccgctg gtttctttgt ccaaacggtg
     2041 tacgatgccc gcgcgcggaa tttgggacag ctcggggcaa tgcgccaaca ggccgttgag
     2101 cagcgtcccc gtccagttgc ccgccgccgg atgcaccacc agtccggccg gtttgttgac
     2161 gacgatgacg gtatcgtctt cgtaaacaat atccaaagcc ataggctctg gaacaaacgc
     2221 cagattttcc tcactcggac ggacggttac acaaatttgc tcgccgccta tcattttgtc
     2281 tttgggttgc gaaggtttat cgtttacaat aaccgcgcct tctttaatcc atgatgtcag
     2341 gcggctgcgc gagtagtcgg gcagaagttt cgccaacacc gcatccaacc gcccgccggc
     2401 aagctcgagc ggaacggtca gaccgatacg gttttcagtt tcggaagctg acgcaaagtc
     2461 tgaatcgtcg ctataatcgg attcattatc aaaggaagta ttctgcatga aaaaaattct
     2521 tttaacggtt tcattaggtt tggcactgag tgcctgtgcc actcaaggta cggtcgataa
     2581 agatgctcag attacccaag attggagtgt ggagaagctc tatgccgaag cccaggacga
     2641 attgaacagc agcaattata cgcgggctgt caagttatac gaaatcttgg aatcgcgctt
     2701 ccccaccagc cgccatgccc ggcaatccca actggatacc gcatacgcct attataaaga
     2761 cgatgaaaaa gacaaggctc tggcggcaat cgaacgcttc cgccgcctcc atccgcagca
     2821 tccgaatatg gattacgcgc tgtatctgcg cggcttggtg ctgttcaacg aagaccagtc
     2881 cttcttgaac aaactggcct cgcaagactg gtccgaccgc gacccgaaag ccaaccgcga
     2941 agcgtatcag gcctttgcgg aactcgtcca acgcttcccg aacagcaaat acgccgccga
     3001 tgcaaccgca cgcatggtca aactggtcga tgcactgggc ggcaatgaaa tgtcggtggc
     3061 gcgttactac atgaaacgcg gcgcatatat cgccgccgcc aaccgcgccc aaaaaattat
     3121 cggcagctat caaaatacac gctatgtcga agaatcgctc gccatattgg aacttgccta
     3181 ccaaaaactc ggcaaaccgc agcttgccgc cgatacgcgc cgcgtgttgg aaaccaactt
     3241 cccgaaaagc ccgtttttga cgcacgcctg gcagcccgac gatatgccct ggtggcgtta
     3301 ctggcattaa gcccatcttc gtaatgccgt ctgaagccgt ttcagacggc attttatgtt
     3361 tcaaacctca ttcaaacggt tttttctgcc aatagaattt ataccctctc aaacgttgag
     3421 ccttcacgcc gccgcgtccc aagccgaatt gcagtgcgcc cgacaaatcg gtacgcagca
     3481 gtttgatgcc gtgtgcgcgg acacggttct gcaccgcttc ggtcggatgt ttgtaggcgt
     3541 tggcataacc gcttgaagca acggcatatt cgggcgagac ggcattgagg aacacgcccg
     3601 acgaggacgt attgctgccg tgatgtccca aaaccaaaac ctggctgtac aggttgcctc
     3661 catacttgcc gaccaggctt tcctcgccct tcgtatccaa atcgcccgtt accagcagtg
     3721 ccgcaccgcc cgccacaaca cgcaaaacac aacttttccc attatcatcg atgtttttgc
     3781 gttcagacgg catcaaaaac tcaaaatcca cgccgtccca ttgccaacgc tgttccgcac
     3841 aatgccgcgc cccctcataa aattccggct gcccggcata aatcccgccg ttcggtatct
     3901 tgccgactgc ctgaaaaccg ccgtcgtggt cgctgtcgtg atgcgacaaa accaaataat
     3961 ccaaatacct gattcccgac gcattcaagc tcggcacaat ccccatctgc gccgccgctg
     4021 ccgttccggt gtcaaacaaa agatgatgat ttgccgtctg aaccgacacc gacaaaccct
     4081 gccccgcatc ccaaaccgta accgcaacct cattttccgg cacgccgggt gaacggtaaa
     4141 acacaaaccc tgccaacagc aacaccgccc acggacgcaa acccaagccg cgcggcagca
     4201 gcaacagcaa agcggcacac accgccaaca ccaacagcgg caaaggtgcg gaggcaacgg
     4261 caaactcggg cgacacatcg gcaagccaca ccaaaaaccg caaagtatat tccgccaaaa
     4321 atgcccccaa ctgttgcaaa ggcgcaaacg gcacgaccga acccagcaac gccagcggcg
     4381 tcaataccca agaaaaccac ggaattgcca ccgcattgac caaagggctg attaaaggca
     4441 gcgaagcaaa cagataaccg agcaaaacca gcgacaacac cgaagccgcc cactgcccgc
     4501 gcaaagcagt ttgccgtttt ccctcgtgca aacgccccga gcacgcccat atcagggccg
     4561 ccaccaaacc gaaagacagc caagtcccca cacccaagac cgccgacggg tcgaacagca
     4621 gcaccgccgc caacgcctgc caccacgtcg cccacgccga caatcttccc ctgcgccaag
     4681 cccacgcaaa cgccgccaac atcaaaacgc tgcgctgcgt cggcacggaa aaaccggcaa
     4741 gcagcgcgta aaacagcgcg cctgcacacc ccgccgccaa aacccacgcg cgcggccggg
     4801 caggaaggcg cggggaacag gcaagcagcc gcttcgccag ccacgcaaac attaccgcca
     4861 ccatcgtaac gtgcaaaccc gaaatactga ccaaatgcgt cagccccaac ggccggaacg
     4921 cctgccacaa ttcggggcgc aatgccgact gttcgcccac gctcaacgcg cgcatcagcc
     4981 cgatgccgtc tgaaagcccg ccgtccgcat ccgcctgctg ccaattacgg ctgatgcggc
     5041 tgcgccaaac cgcaatcccc caaccgcttc cgccatgcag caaaaccctg tccgcaccga
     5101 ccgtccccgc gccgcctatt ccgttggata atgcccacgc ctcacggttc aaaccgcgca
     5161 ggttcaactc cccgacgaca gggtgcacac gtgccgttat ccgccatctg ctcccgaccg
     5221 cccattcgcg ccgtttgtag tccgacagca gcaaatcaaa cgtccgacca ccgctgtcca
     5281 cagcctttgc cgcaaactgc acacgccgcc cgtccgacct cggcatatcc gccacttcca
     5341 ccgtcaaagg cacgcctgaa acggcttctg cccaccattg ggaagacaat gccgcctccg
     5401 tcctgaatac gccgtaagcc gcgcccgcca acacgcacag catcagaccg gcaaacgcaa
     5461 aacgccttgc aaacacagca aaaaccgcaa aagccgccag ccaaaacagc caatgcggca
     5521 cgaccggcag cgcgaacgaa gccgccgcac cgacaaccca acacggcaaa aaccaacgac
     5581 ggaacacgtc tccccacccc agatatttca aaaataaacg gataataaag caaatccgaa
     5641 aaaccgtcat aacaagattt gaaaatgata tagtggttta aatttaaatc aggacaaggc
     5701 gacgaagccg cagacagtac aagcagtacg acaaggcgag gcaacgctgt actggttttt
     5761 gttaaaccac tataagaatg caaacgaaaa gcctcagtat tatccgagac aacggcaaac
     5821 tttccatcgt cattcccgca caagcgggaa tccaaaaaca aaaagccgca gaaatttaac
     5881 ggaagaacca aaccccgaaa accggctccc cgccttcgct tgaataacag aacaaaggaa
     5941 tcagtattat ccgagacaac cgcaaacttt ccatcgtcat tcccgcacaa gcgggaatcc
     6001 aaacttgtcc gcacggaaac ttatcggata aaacggtttc ttagattc
//
LOCUS       DAXHMX010000120         6023 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHMX010000120 DAXHMX010000000
VERSION     DAXHMX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.120"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFM4S_001683"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFM4S_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6051323.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFM4S_001684"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFM4S_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6051324.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAYEHEMAIRHASEAGITGSNDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFM4S_001685"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFM4S_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6051325.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1356 a         1706 c         1613 g         1348 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcaccct ccgccgcata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacaacc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcggcgcgta tgtcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagtttt
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaagg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacctaaatc gaccgcacgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg taggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg ttcgaaccgg ttatgcccgc ttctgatgca
     1561 tgtctgatcg ccatttcatg ctcgtatgca gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa cacggacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcgaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaactcgata
     1861 atcaaaatcg cgtttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccactccagg
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cacttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccggcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattgttg cggtcttgca ggttgatgct cagacccgaa
     2701 ccgttgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg cagacgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactgttgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtat gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgcg ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcagt tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacatata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ccggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcaacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacaaca aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gccggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc agtttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt aatgccggca gatttgattg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagatttg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg ataatgccgc caacttgcgc acggacatcg gcggtacgca
     5761 gcgattccaa acgtcccggc aactcgacgg tcaacgcaac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggagcc ggtgcttccc gacccgcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc att
//
LOCUS       DAXHMX010000121         5994 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHMX010000121 DAXHMX010000000
VERSION     DAXHMX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.121"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            165..1277
                     /gene="nadA"
                     /locus_tag="ACFM4S_001686"
     CDS             165..1277
                     /gene="nadA"
                     /locus_tag="ACFM4S_001686"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6051326.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1329..2837
                     /gene="nadB"
                     /locus_tag="ACFM4S_001687"
     CDS             1329..2837
                     /gene="nadB"
                     /locus_tag="ACFM4S_001687"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6051327.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEASAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLLAQIRRRPHIRVCERQMALDIQTES
                     GVACGLIVLDRRTQETYLIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSESLSSEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(3015..3680)
                     /gene="pgmB"
                     /locus_tag="ACFM4S_001688"
     CDS             complement(3015..3680)
                     /gene="pgmB"
                     /locus_tag="ACFM4S_001688"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6051328.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(3693..5951)
                     /locus_tag="ACFM4S_001689"
     CDS             complement(3693..5951)
                     /locus_tag="ACFM4S_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6051329.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFESEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYTFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
BASE COUNT         1241 a         1672 c         1747 g         1334 t
ORIGIN      
        1 ccaaagcggt ttgtcggaac gaagcgggtc ggttttacgg tatcgcaaac ctgttttcag
       61 acgaccttgc ttattatact caatttgagc ataatataaa aatatttcaa aatataaacg
      121 gcaaatcttt atgccgtctg aaaaaccagc cataaggaag tctcatgcaa accgccgccc
      181 gccgctcgtt cgactacgat atgccactca tccaaacgcc gacttccgcc tgccaaatcc
      241 gtcaggcgtg ggcgaaggtt gccgacacgc ccgaccgcga gacggcagat cgtctgaaag
      301 acgaaatcaa ggctttgctg aaggagaaaa acgcggtctt ggtggcgcat tattatgttg
      361 atccgctgat tcaggatttg gctttggaaa cgggcggatg cgtgggcgat tctctggaaa
      421 tggcacgctt cggcgcggaa cacgaagccg gtacgctggt ggtggcgggc gtgcgtttca
      481 tgggagagag cgcgaaaatc ctctgccctg aaaaaacggt gctgatgcct gatttggaag
      541 cggaatgttc tttggatttg ggttgcccgg aagaggcgtt ttcggcgttt tgcgaccaac
      601 accccgaccg cacggtggtg gtgtacgcca acacttccgc cgccgtgaaa gcgcgtgccg
      661 attgggtggt aacgtcttcg gtggcgttgg aaatcgtgtc gtatctgaaa tcgcgcggcg
      721 agaagctgat ttgggggccc gaccgccacc tcggcgacta catccgccgc gaaacgggcg
      781 cggatatgct gttgtggcag ggttcgtgca tcgtccacaa cgaatttaaa gggcaggagc
      841 tggcggcgtt gaaggcggaa caccccgatg cggtggtgct ggttcatccc gaatcgccac
      901 aaagcgtcat cgaactgggc gacgtggtcg gctcgaccag caaactgctg aaagctgccg
      961 tatcacgccc cgaaaaaaaa ttcatcgtgg cgaccgattt gggcatcctg cacgaaatgc
     1021 aaaagcaggc gcccgacaaa gaatttatcg ccgcgccgac ggcgggcaac ggcggaagtt
     1081 gcaaaagctg cgcgttctgc ccgtggatgg cgatgaattc gctgggcggc atcaaatacg
     1141 ccctgacaag cggacgtaac gaaatcctgt tggacaggaa gctgggcgaa gccgccaaac
     1201 tgcctttgca gcgtatgctc gacttcgcgg caggactcaa acgcggggat gtgttcaacg
     1261 gcatggggcc cgcctgattt gccgtccata ttgccgtttc agacgacctc tccaacaagg
     1321 acaacaccat gcaaaccgat tgcgacgtat tgattgccgg aaacgggctg gcggcactga
     1381 cgctcgccct gtcgctgcct gaatcgttcc gcatcgtcat tttgtgcaaa aaccggctgg
     1441 acgacaccgc cagccgtcat gcgcaaggcg ggattgcggc ggcgtggtcg ggagaggacg
     1501 acatcgaaaa acacgtcgcc gatactttgg aagcgagcgc aggtttgtgc gacgaagccg
     1561 ccgtccgcac catcctgtcg cagggcaaac cggcaatcga atggctgttg gcgcagggtg
     1621 tggcgttcga ccggaatcat aacgacctgc acctgacgcg cgaaggcggg catacctgcc
     1681 gccgaatcgc ccacgtcgcc gactacacgg gcgaagccgt catgcagagc ctgcttgccc
     1741 aaatacgccg ccgcccgcac atccgcgttt gcgagcggca gatggcgttg gacattcaaa
     1801 ccgaatcagg tgtggcgtgc ggactgatcg tccttgaccg ccgaacgcaa gaaacctacc
     1861 tcatccgcgc ccgccatacc gtactcgcag gcggcggctt gggacagatt tacgccgcca
     1921 ccaccacgcc gcccgaatgc acgggcgacg ccatcgccat ggcgatacgc gcaggctgcg
     1981 cagtcgaaaa cctcgaattt atccaattcc accccacagg cttggcaaga tcgtctgaaa
     2041 acggccgcac cttcctgatt tccgaagccg tgcgcggcga aggcggcatc ctgaccaacc
     2101 aagcgggcga acggtttatg ccgtattacg accgccgcgc cgaactcgcg ccgcgcgaca
     2161 tcgttgcccg cgccatcgcc gccgaaatcg ccaaacaaac gcaagacttc gtctcactcg
     2221 atatcagcca tcaacccgca gcgttcgtcc gccggcattt cccgtccatc catcggcact
     2281 gtttatccca atgcggcctg gatatcacgc gccaagccat ccccgtccgc cccgtgcaac
     2341 actacacctg cggcggcatc caaaccgacc cctgcggcag aacttccctg ccgcagctct
     2401 actccttagg cgaaaccgcc tgcaccggct tgcacggagc caaccgcctt gccagcaact
     2461 ccctgctcga atgtgtcgtt accgccagac ttgccgccca aaccatcgca gacggacaag
     2521 cattccaagc caaaacgtct ccaaggccgt ctgaaagcct ctcctccgaa gcaggcatct
     2581 tttcagacgg catccaaagc gcattcagcc gccccgtcct gcaagcgttc aaccaacgcc
     2641 atctgggcat cctccgcaac gataccggtc tgcgctgcgc catcgcccag ctgcggcttt
     2701 ggaaacaaaa ccaaaccgaa ccgcacaccg cgtccgaata cgaaaaccgc aacctgctcg
     2761 aatgcagcct cgccgtcgcc caagccgcat acaggcggag acagaacatc ggcgcgcatt
     2821 ttaatagtga ttgttaaggt tggctggtta gaaaaaaaat cgttcaaata aatttgaata
     2881 gcatggttaa gaaattggca tttagttttt agggttgcgt aagttgggtt atcaatctgt
     2941 tctaaaagca gaaaggccgt ctgaaacact gttcagacgg ccttttactt ccttgacact
     3001 ttatctgacg cgttttacct gcccgactgt gcccacacgt tttgcaggta ggcgtaggtc
     3061 agctcggcgg tatcggacac cagcgcgatg tcgctgccca agtcttcggc tttgcccacg
     3121 ccgataggca aggcgccggc ggctttgatg gcggcgacac ctgcggcagc gtcttcaatg
     3181 ccgatgcagc ggcggatgtc cgcgcccacg ccctcggcgg cggcgaggaa gatgtcgggg
     3241 gcgggtttgg aatgcgcgac ggcggcaggg tcggcgacgg tgtcgaagaa gtgggtcagc
     3301 cccatgcgtt cgagcaggaa ggggccgttt ttactggcgg acgcaagggc gatttttttg
     3361 ccgtttgctt ttaatgcttc cagcagcggc aaaatgccga gatacacgtc ttcgggtttg
     3421 actgcctgaa tcatctcgac gtagttgtcg tttttgcggc gggtcagttc ggcgaactcg
     3481 gcttcgccga cggttttgcc gccgtgtgcg aggatgcgtt tgagcgaatc gtcgcgcgac
     3541 acgcctttga gctgctcgtt aaacttgcgg tctatgccga tgcccagttc ttcggcgagc
     3601 tttttccatg cgcggtagtg gtattcggcg gtgtcggtga tgacgccgtc gagatcgaag
     3661 agcactgcgg taaacgtcat tttgcgccct ccttattttt ccaacgcggc ggtatggctg
     3721 ccgttgagtg tgatgtcttt gccgtacacc tgcaaatcga gcggctcgcc tttgagcagg
     3781 gtgaagacga cgttttcttt gccgacggcg actttaatca gacggcctcg gtagttgata
     3841 tggaaggtgt agcctgtcca cgcgctcggc aggaacggtg cgaagctgag tttaccgccc
     3901 caagttttca tttgggcgaa accttggacg atggcgagcc acgagccggt catagaagta
     3961 atgtgcaggc cgtcttcggt gtcgttgttg tagttgtcca agtccaggcg tgcggtgcgc
     4021 cggtacattt ccacggcttt ttcttctttg cccagttcgg cggcgagaat agagtggata
     4081 cacggcgaca acgagctttc atgcacggtc atcggttcgt agaagtcgaa gttgcggcgt
     4141 ttttcgtcga tattgaaacg gtcgccgaag aaatagatgc cttgcaatac gtccgcctgt
     4201 ttgataaagg gcgaacgcag gattttgtcc cacgaccatt tctggttgag cggcaaatcg
     4261 tcgggcgaaa gcgcggacac ggggcggatg tctttgtcga ggaagccgtc gtgttgcacg
     4321 aatacgccga gttcttcgtc atgaggacgg tacatattcg cgctgatgtc cgcccatttt
     4381 tccaactcgg cggcgctcac gttcaaatcc ggacgcgggt atttcgccag ggcttcgcgg
     4441 gtgtagtcca atacccatgc ggcgagggtg ttggtgtacc agttgttgtt gatgttgttt
     4501 tcgtattcgt tcgggccggt tacgccgtga atcatgtatt tgccgctgcg tttggagaag
     4561 tggacgcggt ccgcccagaa gcgggacact tcgaccaaga cttccaagcc ttctttggca
     4621 agatagcttt cgtcgccggt gtagttggtg tagttgtaga tggcgtaagg aatcgcgcca
     4681 ttgcggtgga tttcctcgaa ggtgatttcc cattcgttgt ggcactcgat gccggtaaac
     4741 gttaccatcg gatagagtgc gcccgccaag ccctgttcgc gcgcgttgtg ctgcgcctgc
     4801 ggcagttggt tgcggcggta ttgcagcagg ttgcgggtaa cttcgggttc cgccagtgcg
     4861 aggtagagcg gtacggcata ggcttcggta tcccaatagg tcgcgccgcc gtatttttcg
     4921 ccggtaaagc ctttggggcc gatgttcagg cgcgcgtctt cgccgtagta ggtggagaac
     4981 agttggaaca gattgaaacg gatgccttgc tgcgcttcgt cgctgccttc gatgaccacg
     5041 tcggcgattt cccaacggtg cagccagcct gctttgtgcg cgtccagcaa ggtttcaaac
     5101 gcaacgcctg cgactttttc cgacaaggcg cggcctgcgg ctttcactgc ttccaagccc
     5161 tgataatcgc ggctggtggt aacaatcacg cgtttttcaa aggttttggg cgtgccgccg
     5221 acttcggact cgaaagaatt ggagacctgc cagtcggttt ggctgccgcc gagggctttg
     5281 aagctgccgg cgaaagtttg ctcggcgttg acgataaatt gttccacgcc gaagggattg
     5341 gcgacggttt gggcggcaat gtaggagaga ctgtctgaaa cgcctttgtc caatacctgc
     5401 cagaattttt cttcgtagtt ggagtcttcg tttttcacgt cggcatcgat gatggaatcg
     5461 atgcggactt ggtgggtttt gccgtcaacg gatacggctt cccagcggat gaccgccaac
     5521 tctttttgcg cgacggacag gaatttacac acatcgaaac gcacgccgaa tacggtgaac
     5581 gagcggcgca acacgccgtg ctgcatatcg agttcgacgg agaagccggc aacatcgttt
     5641 ttcgccaagt ccacctcttg tccgtcgaca aagattttga ctttgctgaa attgagcgcg
     5701 ttgatggctt taccgaaata tttggggtag ccgtttttcc accagccgac gcgggttttg
     5761 tcgggaaacc acacaccggc gatgtaggta cccaagtggc tgtcggcgga ataggtttcc
     5821 tcaaagctgc cgcgcatacc catatagccg ttgcccaaac tggtcaggct ctcttgcagc
     5881 cgtttgtgtt ctttttccag ctttgccgaa cgcagcgtcc aagggctgat ttccataatt
     5941 cttgtgtaca tttatgaagc tcctgtttgg attgatttga gggaatggtg aaat
//
LOCUS       DAXHMX010000122         5962 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHMX010000122 DAXHMX010000000
VERSION     DAXHMX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.122"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(41..964)
                     /gene="truB"
                     /locus_tag="ACFM4S_001690"
     CDS             complement(41..964)
                     /gene="truB"
                     /locus_tag="ACFM4S_001690"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6051330.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(997..1368)
                     /gene="rbfA"
                     /locus_tag="ACFM4S_001691"
     CDS             complement(997..1368)
                     /gene="rbfA"
                     /locus_tag="ACFM4S_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6051331.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            1559..2803
                     /gene="clpX"
                     /locus_tag="ACFM4S_001692"
     CDS             1559..2803
                     /gene="clpX"
                     /locus_tag="ACFM4S_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6051332.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(2906..4102)
                     /locus_tag="ACFM4S_001693"
     CDS             complement(2906..4102)
                     /locus_tag="ACFM4S_001693"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC6051333.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLCRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            4506..5006
                     /locus_tag="ACFM4S_001694"
     CDS             4506..5006
                     /locus_tag="ACFM4S_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051334.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5091..5645
                     /locus_tag="ACFM4S_001695"
     CDS             5091..5645
                     /locus_tag="ACFM4S_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051335.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         1418 a         1587 c         1609 g         1348 t
ORIGIN      
        1 aacacattat tcaaacacag cctgtatttt taaccgccgt tcaggcggcg gatgccgccg
       61 tgttcatcag gcgcaaggct ttcagacggc atatttcttt ttgatattcc gccagaccga
      121 caaagcggcc gttttccgtg taaacgcgta ccggcgtgtc ggaaggcagg tcttcttcga
      181 aacgcggacg ttgtccgcag tggagcatat ggacggcata atcgtttaaa acggtttggg
      241 gaaagtgtga aaccaatacg tcgcagggta gcagcaagct gtcgcgttct gtttcgttca
      301 aatttgccaa ggcctcaagc gtgtggcttt gggcgatggt aaagccggcg gtttcagtgc
      361 ggcgcagggc ggtcaggtgg gcgaacgtgc cgatgtgttt ggcgatgtct tcgctgaggg
      421 tgcggatgta ggtgcctttg ctgcaacgta cgtcgatgac ggctttgggc gcgtcaaatt
      481 cggcaatatc gatggcgtaa acggtaatgt cgcgcgcttt gcgttcgatg acgatgcctt
      541 tgcgggcgta ttcgtacagc ggtttgcctt cgtgcttgag cgcggaaaac attggcggca
      601 cttggcggat gttgcctgtc agtgcttggc aggccgtctg aaattcggct aaggaaatat
      661 cggcgcgggc ggtggcaatg atttcgcctt cggcatcacc cgtgctgctg gcttcgccga
      721 gtttcagcgt ggcggtgtag gctttgtcgg catccagcag gtattgggcg aacttggtcg
      781 cttcaccgaa gcagacgggc aaaagtccgg ttgccaaagg gtcgagcacg ccggtatgtc
      841 cggctttttc ggcatgaaac aaacgccgcg ctttttgcag cgcggtgttg ctggaaaggc
      901 cttcgggttt gtccaagagc aaaacaccgt tgacggcacg tttttggggt ttgcctgtat
      961 tcatggttcg atgttcggac ggcatgggca tatccgtcag tcttcaaccg gtttttccgc
     1021 cgccacttga tcgataaggg cggacaggtt caaaccgcgt tccaaagatt cgtcgtattt
     1081 gaaatgcagt tcgggcgttt tgaacagctt gatgcgtttg gccaattcgc tgcggaggtg
     1141 tccgcgcgcg tgttccagca cttcttccgt aatttcgcgc gcgtcttggt ttaaaatggt
     1201 gtagaacacg gtggcgtggc tgtaatcgcg ggtaacttcg acttcgttga cggtaatgaa
     1261 gccggcgcgc gggtctttca gtccggtacg gacgagttcg gcaagctcgc gcataatttg
     1321 ttctttgaca cggtcttggc gggcgtagcc gcgttggggt tttctcatga atatcctcag
     1381 atttcagatt tggtttcaga cggcattttt gccgccgggg cggttgcgaa agggcgtatt
     1441 atagcctaat cggcgggatt ttaaggtaat acattgataa gaacggatat agttattata
     1501 cttggcaact cgaacggcac gtgccgtttt ctgtaacgca tacattgtaa agaaagccat
     1561 gtccaacgaa aaccgtactt gttccttttg cggaaaatcc aaatcacacg tcaaacattt
     1621 aattgagggc gaaaacgcct ttatctgtga cgaatgcgtc tcaaactgta ttgaaatatt
     1681 gcatgaagac ggaaatgacg gcacgccttc ggaaagtgcc ggaggggagc cggaagaatc
     1741 cggcaagctg cccacgcccg ccgaaatcgt cgccaacctc aacgaccacg ttatcggtca
     1801 ggaacaagcc aagaaagcct tggcggtgtc ggtgtacaac cattacaaac gcctgcgcca
     1861 tcctaaagca ggagctaatg tcgaattgtc gaaatccaac atcctgctta tcggcccgac
     1921 cggatcgggt aaaaccctgc tggcgcaatc tttggcgcgc aaactggatg tgccgttcgt
     1981 gatggcggat gccacaactt tgactgaagc aggttatgtc ggcgaagacg tcgagcaaat
     2041 cattaccaag cttttgggca aatgtgattt cgatgtcgaa aaagcccagc gcggcattgt
     2101 ctatatcgac gaaatcgata aaatttcacg caaaagcgac aacccgtcga ttacccgcga
     2161 cgtatccggc gaaggtgtgc aacaagcctt gttgaaactg attgaaggta cagtggccag
     2221 cgttccgccc caaggcggcc gcaagcatcc gaatcaggaa tttatcaacg tcgataccac
     2281 caacatcctg tttatctgcg gcggcgcgtt tgcaggcttg gaaaaagtca tccgccagcg
     2341 tactgaaaaa ggcggtatcg gtttcggtgc gtccgttcac agcaaggacg aaaatgccga
     2401 cattaccaag ctgttcggca tcgtcgaacc ggaagacttg attaaattcg gtctgattcc
     2461 agaattaatc ggacgtttgc ctgtgattgc aactttagaa gaattggacg aagacgcgct
     2521 gattaatatt ttgaccgagc cgaaaaatgc tttggtcaag cagtatcagg cgttgttcgg
     2581 tatggaaaat gtcgaactgg agtttgaaga aggcgcattg cgttccatcg cgcgtcaggc
     2641 aatggaacgc aaaaccggcg cgcgcggcct gaggtccatc gtcgaacgct gcctgctcga
     2701 tacgatgtac cgcctgcctg atttgaaagg cttgaaaaaa gtggtggtcg gtaaggcagt
     2761 catcgaagag ggcagggaac cggaattggt gttcgagtcc tgatggcaaa caaaaccgct
     2821 tcggaacgtt gaaccacgtt tctgaagcgg ttttttgttt ctgatgccgt ctgaagcctt
     2881 cccgccttca gacggcattt gagttttacg gattgtctga tgtcgcggca aattccgtca
     2941 gcgcgtgttc caaacgtcgc aaaccttccg ccatatcctc atcgttcaac agtagcgaag
     3001 gcgcgaaacg caatacgtcc gcacccgcaa ccaaaatcat cacgccgtgt ttcaaggcgg
     3061 cggcggtgat ttcggatgcg cgtccgcgat aggcttcgtc caacacgcag ccgagtagca
     3121 gccccatccc gcgaacttgt gagaacaagc ccgttttcct gcacaaatcc agcaatgccg
     3181 tctgaagttt ctgcccctgt tcacggacat ggtttaaagt ttcgggcgta ttgatgatgt
     3241 cgaatgcgcg gctgccgacc gcacacgcca tcgggttgcc gccgaaagtc gagccgtgcg
     3301 tgcccggttg gaaggcggcg gcaatctttt ctgtcgccag catcgcgccg atcggaaagc
     3361 cgcagcccaa ggcttttgcc gaactcaaaa tatcgggaac aacgccgtaa tgttcatagg
     3421 caaacagcct gcccgtatgc cccatcccgg tttgaacttc gtccaaaatc aacaacgcat
     3481 tgtgccggtc gcacagacgg cgcgcggttt gcaaatattc cgcagtggcg ggcaggatgc
     3541 cgctttcgcc ctgtatcggc tcgatgatga ccgcgcaggt ctgttcgccg acggcagctt
     3601 ccagcgcggc aatatcgttg aacggaacgt gcgtaatgcc ttgcggcagc ggtgcataat
     3661 ccttgctgta tttcggctga ccgccgacgg acacggtaaa cagcgtgcgt ccgtggaaac
     3721 tgttgataca ggcgacgatt tcgctttttc ctccgccgaa acggtcgcgc gcatatttcc
     3781 ttgccagctt caacgccgcc tcattcgctt ccgcgcccga gttgcagaaa aaaaccttgt
     3841 cggcaaaact gtttgcaacc aatttttgcg ccaattcctg cgccggacgc gtcgtataga
     3901 tattggaaat gtgccacagc ttgtgcatct gcgcgtttaa agcatcggca agggcagggt
     3961 ggcagtgtcc cagcgcattg acggcgatac cgcctgaaaa atcaatatat tcacgccctt
     4021 ccgtatccca aacgcggctg cctgaagcgc gttcgggaat catcggggca aaggcgaaat
     4081 tgggggtcag atagttttgc ataattgctc ctgattgcgg ttttgctcgg gattatgcac
     4141 cgtttttcaa agcagataaa gggaaattgc cgacaatatc gggcagcggt aaaaataaac
     4201 cgaaatattt tcaatcggat agatgaaaaa tcaaaaaaat ttcgataaaa tgcttgaggc
     4261 gcggcagcgt tttgatttag aatctcccca atatttttat cttcagacgg catttcatca
     4321 tgcaaggcaa aaaatttaca accgcttatt gctattggta ccacacagac gtgcggtact
     4381 tttttgcgct tgcttgaaat ccatcccata cgcaaaacag gccgctcgaa aatggggcgg
     4441 cttttttgtt accttcacac aggtcgcccg attcggcaac acacgacaga atcaggaaaa
     4501 tgaccatgaa tgcaatgccc caaaccgccg cacaaaacga aaccctgcac acttgtagca
     4561 ttattgtcga tgatgcgcag gatttttccc ccatgcttct cgatgcaggg cgggacgaca
     4621 cacttatcag cgaatcgatg attccgcaaa tccgcaccgt tgccgccctg atagccgccg
     4681 aacggcacga cttcagccgc agcagccccg ccgagtttac cgatgccgcc gatttttttg
     4741 ccgcacggat tctcgtattg ggcgtgcgac gcttccatct ggacgtatcg ctgcttcaga
     4801 ttttgaaaac cgccaacagc cgcgcccgcc gctttgccga aaaacaccgc ctgtttttca
     4861 cacccgcaca agccgaattg agcctgaaca aacacaaaaa ccgccgcctg ctgaccgtag
     4921 aaaccgaaca cgaagtcgaa aacaaaggca acccagtcgc caacagcctc gcatttgtcc
     4981 gcaaactgca caccctgccc ctgtaaacat cacgatttgt cggcagaatg cttgccaaaa
     5041 ttcccgacgg cgatacaata aaaccgattt tccacccaaa caggacacac atgaaaaaaa
     5101 tcatcgcctc cgcgcttatc gcaacattcg cactcgccgc ctgccaagac gacacgcagg
     5161 cgcggctcga acagcagcag aaacagattg aagccctgca acagcagctc gcacagcagg
     5221 cagacgatac ggtttaccaa ctgactcccg aagcagtcaa agacaccatt cctgccgaag
     5281 cacaggcaaa cggcaacaac gggcaacccg ttaccggtaa agacgggcag cagtatattt
     5341 acgaccaatc gacaggaagc tggctgctgc aaagcctggt cggcgcggcg gcaggcgcgt
     5401 ttatcggcaa cgcgctggca aacaaattca cacgggcagg caaccaagac agtcccgtcg
     5461 cccggcgcgc gcgtgcagcc taccatcagt ccgcacgccc caatgcgcgc accagcaggg
     5521 atttgaacac gcgcagcctc cgcgcaaaac aacaggcggc gcaggcgcag cgttaccgcc
     5581 cgacaacgcg cccgcccgcc aattaccgcc gaccggctat gcgcggtttc ggcaggaggc
     5641 ggtaaacccg gcgcgccaat gccgtctgaa gggctttcag acggcatttt gtttccaaga
     5701 gtttgatgtc gggatggcaa ttctgattta cgtgcgttgg cagggcggca ttgtcatgcc
     5761 gccgttgggt tttcagacgg catcatatcg aaccgtcagg tcgtggggtg agcattcttg
     5821 cccaccgttg taagggcggg attttttggt gaacaggaat gcccaccctg catctgaaac
     5881 ccttctgcgt cattcccacg caggaaggaa tttagatatt tgaatttaca gaaattttca
     5941 atcgttgcca aagcggaaaa gg
//
LOCUS       DAXHMX010000123         5880 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHMX010000123 DAXHMX010000000
VERSION     DAXHMX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.123"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(147..1190)
                     /gene="argC"
                     /locus_tag="ACFM4S_001696"
     CDS             complement(147..1190)
                     /gene="argC"
                     /locus_tag="ACFM4S_001696"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6051336.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(1187..1468)
                     /locus_tag="ACFM4S_001697"
     CDS             complement(1187..1468)
                     /locus_tag="ACFM4S_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051337.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSTANQNPVGQRSDGIPANHTKEKP"
     gene            1836..2990
                     /locus_tag="ACFM4S_001698"
     CDS             1836..2990
                     /locus_tag="ACFM4S_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6051338.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAARNLEAASWKDINEI
                     YQRADPTAPLFYLRKKGAIVPKEEYVESFDALGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHISRQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            2999..3205
                     /locus_tag="ACFM4S_001699"
     CDS             2999..3205
                     /locus_tag="ACFM4S_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051339.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQ"
     gene            3410..3670
                     /locus_tag="ACFM4S_001700"
     CDS             3410..3670
                     /locus_tag="ACFM4S_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6051340.1"
                     /translation="MERKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYY"
     gene            3830..4456
                     /locus_tag="ACFM4S_001701"
     CDS             3830..4456
                     /locus_tag="ACFM4S_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HFC6051341.1"
                     /translation="MNRPKQPFFRPEVTVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITTKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRAANLFIL"
     gene            4360..4608
                     /locus_tag="ACFM4S_001702"
                     /pseudo
     CDS             4360..4608
                     /locus_tag="ACFM4S_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFP transporter"
     gene            4921..5223
                     /locus_tag="ACFM4S_001703"
     CDS             4921..5223
                     /locus_tag="ACFM4S_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051342.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5507..5659
                     /locus_tag="ACFM4S_001704"
                     /pseudo
     CDS             5507..5659
                     /locus_tag="ACFM4S_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
BASE COUNT         1584 a         1259 c         1430 g         1607 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacgggattt tagatttttg ttttcgcgga ataatgagcc
       61 aaaagccgtc tgaacctaaa aaaccgccac tttcagatgg catcaaaaaa ccgcaggcac
      121 gatacctgcg gtttgccgtt tgcgtttcag gggagcaggg ggattgcgtc caagccgtgt
      181 gtttccttca gtccgaacat gatgttcata ttttgcaccg cctgacccgc cgcgcctttg
      241 acgaggttgt cgatgacgga aaggacgacc cacacatcgg attgcgccgc ctgttggatg
      301 ctgatgcggc agaggtttgc gccgcgcacg ctgcgggttt cgggtgtcga accggcgggc
      361 aggatgtcca cgaacaggct gtcgcggtaa tagtcgcgca ggatggtttc ggggcagatg
      421 ccgtctgaaa ggtggaggta aacggtggcg tgcataccgc gtatcatcgg cgcgaggtgc
      481 ggcgtgaaca cgaatccttc ggcgatgccg tcctgaagcc cggcgatggt ctgcctgatt
      541 tcgggcaggt ggcggtgtcc agctatgccg taggctttga agttgtcgcc gacttcgcac
      601 aacagcgaac cgacattgcc tttcctgccc gcgccggaca cgccggattt gcagtcggca
      661 atcagcggca taccgggctt cagacggcat tgccgcaaca gcggcacgag cggcagggat
      721 acgcaggtcg ggtagcagcc ggggttggcg acgaggcgtg cctgtgcgac ggcttcgcgg
      781 ttgagttcgc tcaatccgta cacggcttgg gaaacgaggt cgggggcggc gtgggtcatg
      841 ccgtaccagt gttcccaggt cggaatgtcc cgtatgcgga agtcggcgga aaggtcgatg
      901 acgcgcacgc cctgttcaat caggcgcggc gcgtctttca tggcgatgcc gttgggcgtg
      961 gcgaagaaga cgatgtcgca ttgttccaaa cctgcctcgt cgggcgtttg gaaggcgagg
     1021 ccgtacacgc cgcgcaaact cggaaagtaa tcggcaactg cagttcccgc ttcgctgcgg
     1081 ctggttacgg cggcgacttc gacatcggga tgggcggcaa gcaggcgcag cagttccacg
     1141 cccgtgtagc ccgtcgcgcc gacaatgccg actttgattt ttttactcat ggtttttcct
     1201 ttgtgtggtt ggcgggtatg ccgtccgaac gctgtccgac ggggttttga ttggcggtgg
     1261 aaggacggta cacaaagcgt ttgaaaagcg tgtctaattt aatggcatcc gcctctgttt
     1321 ttaaaaccca gccctgccgt ccggaataaa cataaccgta ggcgcgaaag tcggcaggat
     1381 atttcggtgc ctgccccaaa cccaaaccgt atgcggcgaa agcggtggtc gtggagaagg
     1441 aaagcaggag gaggacgagg gttttcataa ggttccaaac cggacggggc gatgaagccc
     1501 gattatagca aaaggtcgtc tgaaaaccgt tttcagacga ccttttattt aattacaata
     1561 aaaatgttaa caacaaaaaa caaaccgctt tttttcgttt ggaaacaaaa caaattttta
     1621 tagtggatta aatttaaacc agtacagcgt tgcctcgcct tgccttgccg tactatctgt
     1681 actgtctgcg gtttcgtcgc cttttcctga tttttgttaa tccactataa ctaaaattgt
     1741 caacaattcc ttgacgcaca cacaaaaaaa actttaatat ttgtaaatat atgtaacatt
     1801 ataaatttta aatattttct gagtaagggg aagtaatgag catccatctc gacttcggca
     1861 ttagtcctaa aacgttccga cagacttatc tgtatcaaaa gcccaagctc tttaaaggag
     1921 cggctcggaa tctcgaagcc gcatcttgga aagatatcaa cgagatatac caacgagcag
     1981 acccaaccgc accgctgttt tatctgcgta aaaaaggtgc aattgttcct aaagaagaat
     2041 acgtcgaaag tttcgacgct ttgggcaaaa ctcgctaccg ttttattaaa tccgttatct
     2101 acgaacatat gaagaatggt gcgtcgttag tctataacca tattaacaac gagccgtttt
     2161 cagatcatat ttcccgtcaa gtcgcccgct ttgctggcgc acatactatt gtcagtggat
     2221 atcttgcttt tggcagcgac gaatcttata aaaaccattg ggacacccgc gatgtgtatg
     2281 ccatccagct tttcggcaag aaacgttggc aacttactgc ccctgatttc cctatgccat
     2341 tgtatatgca acagactaaa gatactgata tttccattcc tgaacatatc gatatggata
     2401 ttatccttga agcaggtgat gtcctctaca tcccgcgcgg ttggtggcac agacctatcc
     2461 cgctcggctg tgaaaccttc cacctcgctg tcggtacctt tccgcccaac ggctataatt
     2521 acctcgagtg gctaatgaag aaattcccca cgatagaaag tctgcgccac agtttctcag
     2581 actgggagca agataggacg cgtatcaacg atactgccgc acaaattgct gccatgattg
     2641 ccgaccccgt caattacgaa gccttcagtg aagacttcct cggcaaagaa cgcaccgata
     2701 ccgcttttca tctcgaacag ttcgcgaatc ccaacgctac tccgctttca gacgacgtca
     2761 ggttgagact aaatgccaat aatttggata cgttggaaaa ggggtatttg attgggaatg
     2821 ggatgaagat aagcgtagat gaattgggga aaaaagtttt agaacacatc ggtaagaatg
     2881 aaccgttatt gttgaaaaat ctactggtta acttcaatca gggaaaacat gaagaagtta
     2941 ggaagttgat ttatcagttg atagagttag attttctgga acttttgtga gggattctat
     3001 gaaaaactgg aagcaattta catttttcgt aatattagta atagcttgtt atcaagtgct
     3061 atatttttta tcagatatgt ttcttctcga ctacattaac aaatatagtt ggaatttgaa
     3121 ttttattcag ggtactttga attttttttc aatatatctg ccatatgttt ttgttagccg
     3181 aatttttagg aatactaacc aatgaaagac taggctatga aatcaaaatt tatgtataag
     3241 ttactggtac ttgcaaagta cctgtatatt ttaatttgta tatcaatacc aatcgttttt
     3301 atttatgaaa gctaccaagg agatgatgtt gattttatga tttatattca tgcttttatt
     3361 attttttcca tattgggtgt attgactcaa tattctacgt ctaatttttt tggaaaggaa
     3421 aaataacatc attgaactat ccattgagaa tctggagcta atttatggcg gaggcttagg
     3481 ggagagaatc cttgaaggag gagcaaatgg tgcacaaata ggaggatcaa ttggtgcagg
     3541 tattggtctg ggtgtcggta tagtttctgg tggtattcct gccggaccgg ctattattct
     3601 cggtggcaca gttggtgctg ctgttggtgc tgtaactggt atgggtgttg cagcatatga
     3661 gtattactaa gaaactcagc ccaaagggac agtaacggtc ggtccgattt ctgcttttgc
     3721 tgtggacaaa tcaggaaacg gctacactga cggtacagat tactgctgat atttctcatc
     3781 tttcagacga tctctagggg tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc
     3841 caagcaacct ttcttccgcc ctgaagtcac agttgcccgg caaaccagcc tgacgggtaa
     3901 agtgattctg acacgaccgt tgtcattttc cctatggacg acatttgcat cgatatctgc
     3961 gttactgatt atcctgtttt tgatatttgg taactatacg cgaaagacaa cagtggaggg
     4021 acaaatttta cctgcatcgg gcgtaatcag ggtgtatgca ccggatacgg ggacaattac
     4081 aacgaaattc gtggaagatg gagaaaaggt caaggctggc gacaagctat ttgcgctttc
     4141 gacctcacgt ttcggcgcag gaggtagcgt gcagcagcag ttgaaaacgg aggcagtttt
     4201 gaagaaaacg ttggcagaac aggaactggg tcgtctgaag ctgatacacg agaatgaaac
     4261 gcgcagcctt aaagcaactg tcgaacgttt ggaaaaccag aaactccata ttttgcaaca
     4321 gatagacggt cagaaaaggc gcattagact tgcggaagat cagtggcaaa aacggcactg
     4381 ggcagacagg aattgtcggg attgggcatg gtatcctccg atttggcgaa gagcagcgaa
     4441 cctgtttatc ttgtgaaaat aaaacccgac aaatcaacca tcactgcata cggtgaggaa
     4501 aaaccgctgc aaatcggcat gacgttggaa gcagacatcc tgcacgagaa acggcgattg
     4561 tacgaatggg tattggagct gatttatagt atatcgggca aactgtaaga cttgaggctg
     4621 aaagataaag aagttgtctg aaaccttttc agacgaccag aagacaatat ataaaaacac
     4681 aaacttcaaa actgcctgaa accatcctct tttacaggca ctccgtctgt aaaagcaagg
     4741 atagggcatc tgcccttttg attgtttgat taacgataca aggagtttca aaatgagagt
     4801 tttatagtgg attaacaaaa accagtacag cgttgcctcg ccttgccgta ccatttgtac
     4861 tgtcttcggc ttcgtcgcct tgtcctgatt taaatttaat ccactatatg tgttcatgaa
     4921 atgacttggg tcggaggctc aggtaatgcg caacaaagtt catattattg cgaaatttgc
     4981 gaatctgcag ggcttaacga tacgggaaat cctgataaat ctttaggatt gccaaacaat
     5041 acgttcagta atccgcctgg ttggggagct acaatcggag ctttagcagg tagccgcata
     5101 gggatgcctg aatttggtac gtttgcgagc catgccattg aaaatttcga ctggtcatgg
     5161 tatcgacgtt atagggaaat tgccgaaacg attgaacgag aatattcagg cggtttgcct
     5221 taatagttga ggaggtcatg atgtttgcca aacattatca attcatcgca ctcggcatca
     5281 tgctgcttct ttatatgttg attctctata cgaccgattt ttccaatctg acgtattgga
     5341 tgctgttttt tatctgtttt attacaggaa aaatattagc tcgtttgtta gagaaaagct
     5401 ttaaataaaa tagcagctag tcgcaaaagg tcgtctgaaa ccttttcagg cggcctttct
     5461 aaaatacatc caacttccta atccctattt ttcaaaaagg aaatctatgc cccatctgca
     5521 aaacctgtct ttgggcttaa agaaaaagct gcctgttatc ctgcaaacag aaatatcaga
     5581 atgcggcttg gcatgtctgg cggctgtggc gggatttcat ggtttccata cgaatttacg
     5641 cgcactgcgt tcaaaatact gtccgagacc tttgcaaaat tccccaaaat cccctaaatg
     5701 tcttggtggg aattttgggg aattttgcaa aggtctcatt ctataactgt aaatactttt
     5761 aaatttatga caaaatagta aatattgcta aaataatatt gatgtcatga aattttttcc
     5821 tgctccatgt ctgttggtta tcctggctgt catacccctt aaaaccttag ctgccgatga
//
LOCUS       DAXHMX010000124         5867 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHMX010000124 DAXHMX010000000
VERSION     DAXHMX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.124"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            20..403
                     /locus_tag="ACFM4S_001705"
     CDS             20..403
                     /locus_tag="ACFM4S_001705"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC6051343.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            517..990
                     /locus_tag="ACFM4S_001706"
     CDS             517..990
                     /locus_tag="ACFM4S_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6051344.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            1116..2219
                     /gene="mnmA"
                     /locus_tag="ACFM4S_001707"
     CDS             1116..2219
                     /gene="mnmA"
                     /locus_tag="ACFM4S_001707"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6051345.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYEGDVCLGGGIIQSTDKPVIITR"
     gene            2290..3960
                     /locus_tag="ACFM4S_001708"
     CDS             2290..3960
                     /locus_tag="ACFM4S_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6051346.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4072..5706
                     /locus_tag="ACFM4S_001709"
     CDS             4072..5706
                     /locus_tag="ACFM4S_001709"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6051347.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1535 a         1598 c         1484 g         1250 t
ORIGIN      
        1 acattttgaa aggataccga tggaaccttc ctcctacgcg gcagaaaaaa aaggcaaagg
       61 cggcatcagg cgcgtcatta acgcattcgg ctattcgata gacggcatcg ccgccgccta
      121 ccgttacgaa gcggcattcc gtcaggtttt gtggctgaac gtactgctgg tgtgcgcggc
      181 atttttttgg gtttccgaaa ccgccgtccg cctgccgttg attatcgcgt cttttgtgtc
      241 ggtcattgtc gaactgttca acaccgccgt cgaagctgcc gtcgatcata cttcgaccga
      301 aaaacacgag ctggccaaac gcgccaaaga cgcaggttct gccgcacaat tgttcgcgat
      361 gctgatgttg gcggcggttt ggctgtctgc cctgttcggg taaaacgctt gcagcaggat
      421 ttgtaatcct ttaggattag tatgtggtat ttattattca cttaatccac tataagatta
      481 agcacattcc ccgtctgata ttcaaggagc agaaagatga aaaaattatt ggcagccgtg
      541 atgatggcag gtttggcagg cgcggtttcc gcggccggag tccacgtcga ggacggctgg
      601 gcgcgcacca ccgtcgaagg tatgaaaata ggcggcgcgt tcatgaaaat ccacaacgac
      661 gaagccaaac aagacttttt gctcggcgga agcagccccg ttgccgaccg cgtcgaagtg
      721 catacccaca tcaacgacaa cggcgtgatg cggatgcgcg aagtcaaagg cggcgtgcct
      781 ttggaggcga aatccgttac cgaactcaag cccggcagct atcacgtgat gtttatgggt
      841 ttgaaaaaac aattaaaaga gggcgacaag attcccgtta ccctgaaatt taaaaacgcc
      901 aaagcgcaaa ccgtccaact ggaagtcaaa accgcgccga tgccggcaat gaaccacggt
      961 catcaccacg gcgaagcgca tcagcactaa tctgctgaaa atatttgaaa tgccgtctga
     1021 aaaagcccgg gctttcagac ggcattttta tgactgcctt taaaatgtgt taaaatccgc
     1081 ctttaaaaac cgccgtttcc aaccatattc tacgcatgaa tacgacagca aacccttcca
     1141 atatcatcgt cgggctctcc ggcggtgtcg attcttccgt aaccgccgcc ctgctcaagc
     1201 agcagggtta tcaagtgcgc ggtgtgttca tgcagaactg ggaagacgac gacaacgacg
     1261 aatattgcag catcaaacag gattcgtttg atgccatcgc cgttgccgat attgtcggca
     1321 tcgacatcga catcgttaat ttcgccgcgc aatataaaga caaagtcttt gcttattttc
     1381 ttcaggaata cagtgcgggg cgcacgccga atcccgatgt gttgtgcaac gccgaaatca
     1441 aattcaaatg ctttttggat tacgccgtag ggcagggcgc ggataccatc gccaccggac
     1501 actatgcgcg caaagaagtc cgcaacggcg tgcattacct gctcaaaggt ttggatcgaa
     1561 acaaagacca aagctatttc ctctaccgcc tcaagccttt ccaactcgag cgcgcgattt
     1621 ttccgttggg cggtttggaa aaacccgaag tccgccgcct tgccgccgag ttcaaactgc
     1681 cgactgccgc caaaaaagac agcacgggca tctgcttcat cggcgaacgc ccgtttcgcg
     1741 agtttctgca aaaatacctg ccgaccgaca acggtaaaat ggttacgccc gaagggaaaa
     1801 ccgtcggcga acacgtcggg ctgatgttct acacgctcgg acagcgcaaa ggtttgggca
     1861 ttggcggcgc gggcgaaccg tggtttgttg cggctaaaga tttgacgaaa aacgaactga
     1921 ttgtcgtgca aggacacgac catccgctgc tctatacccg cagccttgtg atgaacgatt
     1981 tgagtttcac gcttcccgaa cgcccgaagg caggacgcta tacctgcaaa acgcgttacc
     2041 gtatggcgga cgcgccttgt gagttgcgct atttggatga agaaaccgcc gagctggtgt
     2101 ttgacgaacc gcaatgggcg gttacgccgg gccagtctgc cgtgctgtat gagggcgatg
     2161 tctgcttggg cggcggcatt atccaatcaa ccgacaaacc cgtcatcatc acgcgataaa
     2221 ggtaatgccg tctgaaacgg ttttcagacg gcattgttcc gctcaattcc actttaaaga
     2281 ccgataccta tgaaaaaaat ctggttagac agctacgaga agggcgtcag tgccgaaatc
     2341 gacatcacgc aatacaattc cgtcagcgac gtattccgcc aaagcgtgga aaaatttgcc
     2401 gaccaacctg cttttcaaaa catgggtaag acgctgactt atgccgaaac cggcaaactg
     2461 gcaacagatt ttgcttctta tctgcaaaac gtccttaaat tgcctcgagg agagcgcgtg
     2521 gcaataatgt tgcccaatgt actgcaatat ccggttgccc ttttcggcat tttgcaggca
     2581 ggtttggtag cggtgaacac caatccgctc tataccccgc gcgagctgga gcatcagttg
     2641 aaagacagcg gcgcgaccgc catcatcgtt ttggaaaatt tcgccaacac gctggagctg
     2701 gtgctgccgc gcacgcagat caaacacgtc atcgtcgcct ctgtcggcga aatgttcggg
     2761 ctgcttaaag gttcgctgat caatttcatc atccggaaaa tcaagaaaat ggtgcccgaa
     2821 taccgtattc gggaaaccat ttcctttcag acggcattga aagaaggggc gaagcacgtt
     2881 ttccaacctg tcgcattaaa ccgcgaagat accgcgctgt tgcaatacac gggcggcacg
     2941 acaggtgttg ccaaaggggc ggtgctgagt cacggcaaca tctgcgccaa tatgcttcag
     3001 gcaaaagaat ggattaaaaa ccagttgcgc gagggcaaag aaaccgttat cgccgccctg
     3061 ccgctgtatc atatttttgc cttaaccgtg aatctgatga tttttgccaa tgccggctcg
     3121 aaaatcatcc tgattaccaa cccgcgcgat atgaaaggct ttatcggaga actgaaaaaa
     3181 cagcgggtta atgtatttat cggcgtgaac acgctgttta acgcgatggt taaccgtcct
     3241 gattttgccg aagtcgattt ttcaggattg cggctgactt tgggcggcgg tatggcgacc
     3301 caaaaagccg ttgccgaaaa atggaaaaaa atcaccggca cgcccatcgt cgaagcctac
     3361 ggtctgaccg aagccagccc cggcgtgtgc tgcaacccct taaacatcga atcatacagc
     3421 ggcagcatcg gtttgcccgt ctcgtccacc gaagtcgaac tgcgcgacgc aaacggcaaa
     3481 gaagtccccg tcgggcagcc gggcgaattg tgggtaaaag gccctcaagt gatgcaaggc
     3541 tactggaacc gccccgaaga aaccgccaaa gccatagacg cgcgcggctt cttggaaacc
     3601 ggcgatattg ccgtcatgga cgaaaaaggc tggttgaagc tggtcgatcg taagaaagac
     3661 ctcgtcgttg tttccggatt caatgtttat ccgaacgaaa tcgaggaagt catcgcgcat
     3721 cacggcaaag tcatggaagt cgcctgtatc ggcgttcccg acgaaaaaac cggcgaggca
     3781 ctcaaagtat tcgtcgtcaa aaaagacccg tcattgacca aagaagaact tatcgccttt
     3841 tgccgcaccg aattaaccgc atataaagta ccgaaaaata tcgaattccg cgacgagttg
     3901 cccaagtcca acgtcggcaa aatcctgcgc cgcgagttgc ggcaaagtac cgggaaataa
     3961 agaaaagatg ccgtctgaaa acagccgtcc accgttcaga cggcatccgt ccgtttgcca
     4021 gaaccgcgcg cttcacgtta aaatcacgca ttccaacatg ggtattccat catgaccaaa
     4081 ttcattttcg taaccggcgg cgttgtctcc tcactgggta aaggtatcgc cgccgcttct
     4141 attgccgcca tcctcgaatc gcgcggcttg aacgttacca tgctcaagct cgatccttat
     4201 atcaacgtcg atcccggcac gatgagcccg tttcaacacg gcgaagtgtt cgtaaccgac
     4261 gacggcgcgg aaaccgacct cgacttggga cactacgaac gtttcatcga ttccacgatg
     4321 acccgccgca acagcttcag cacggggcag gtgtacgaaa acgtcatcgc caaagaacga
     4381 cggggcgact acctcggcgg tacggttcaa gtcatcccgc acattaccga cgaaatcaaa
     4441 cgccgcatcc acgaaggcgc ggcaggttac gatgtggcga ttgtcgaaat cggcggtaca
     4501 gtcggcgata tcgaatcttt gccattcttg gaagccatcc gccaaatgcg cagccagctc
     4561 ggccgcaaca atacattgtt tgcccacctg agctatgttc cttacatcgc cgctgcaggc
     4621 gaaatcaaaa ccaagccgac ccagcacacc gttaaagaaa tgttgagcat cggtttgcaa
     4681 cccgacatcc tgatttgccg tatggacagg acaatgcctg cggacgaacg ccgcaaaatc
     4741 gccttgttct gcaacgtgga agaacgcgcg attgtcggca gctacgatgt ggacagcatc
     4801 tacgaatgcc ccgaaatgct gcacgaccaa ggcatcgata acatcattac cgagcagttg
     4861 cagcttaatg tgcagcaggc ggatttgacc gcgtggaaaa aaatcgtcca cgccatccaa
     4921 aacccgaaac acaccgtcaa aatcgccatg gtcggcaaat acgtcgattt gaccgagtcc
     4981 tacaaatcgc tgatcgaagc cttgaaacac gcaggcattc acaccgaaac cgacgtacaa
     5041 attacctttg ttgacagcga aagcatcgag aaaaacaacg gcgacgtttc tatgctcaaa
     5101 gatatggacg ccatcctcgt tcccggcggt ttcggttcgc gcggcgtgga aggcaaaatc
     5161 gccgctgtgc gctatgcccg cgaaaacaac gtgccatact tgggcatctg cctcggtatg
     5221 cagattgcgc tgatcgaata cgcccgcgac gtggcaggtt tgaaaggtgc gaattccacc
     5281 gagttcgacc tcaaatgcgc cgcccccgtc gttgccctga ttgacgaatg gcaaactgcc
     5341 gacggcagcg tcgaaacccg cgacgaatcc gccgatttgg gcggcaccat gcgtttgggc
     5401 gcgcaagaag tcgaattgaa agcaggcagc ctcgccgcca aaatctacgg cagcgaacac
     5461 atccgcgaac gccaccgcca ccgctacgaa gtcaacaaca actacgttcc tacgctggaa
     5521 caggcaggtt tggtcatcgg cggcgtatcc gccggacgcg aacgcttggt cgaaaccatc
     5581 gaactgccga accatccttg gttcttcgcc tgccagttcc accccgagtt tacctccaat
     5641 ccgcgcaaag ggcatccttt gttcaccgcg tttgtcaaag ctgcgttgaa caataaaaaa
     5701 gcctgataaa gcgttacttg atgatcaaaa tgccgtctga aagcctgaat gaagactttc
     5761 agacggcatt tttgcaaatc ggggatcata aaagccatca atttaatttt tatggtttta
     5821 tctaatattg accaactgaa tattgttaag cggggcgtga aactcaa
//
LOCUS       DAXHMX010000125         5866 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHMX010000125 DAXHMX010000000
VERSION     DAXHMX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.125"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            41..454
                     /locus_tag="ACFM4S_001710"
     CDS             41..454
                     /locus_tag="ACFM4S_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6051348.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            500..1129
                     /gene="nth"
                     /locus_tag="ACFM4S_001711"
     CDS             500..1129
                     /gene="nth"
                     /locus_tag="ACFM4S_001711"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6051349.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1267..2766
                     /locus_tag="ACFM4S_001712"
     CDS             1267..2766
                     /locus_tag="ACFM4S_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6051350.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGKEITVAVKLGN
                     ASEQTGSSSEPDKAPYAEHQSGTFSVESAGITLQTHTDSSGGRLVVVRVSGAAERAGL
                     RRGDEILAVGQVPVNDEDGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            2969..4489
                     /locus_tag="ACFM4S_001713"
     CDS             2969..4489
                     /locus_tag="ACFM4S_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6051351.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4546..5631
                     /gene="nagZ"
                     /locus_tag="ACFM4S_001714"
     CDS             4546..5631
                     /gene="nagZ"
                     /locus_tag="ACFM4S_001714"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6051352.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEERQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQEAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5712..5788
                     /locus_tag="ACFM4S_001715"
     tRNA            5712..5788
                     /locus_tag="ACFM4S_001715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5746..5748,aa:Pro,seq:cgg)
BASE COUNT         1325 a         1805 c         1489 g         1247 t
ORIGIN      
        1 caatccgtcg ccgatttttg cctgtttgag actgtttgca atgatgcccg cctacgccta
       61 cgccgcgctc gccttttccg ccttcacttc cgccaccctc ctgcccggca catccgaagc
      121 cgcctttgcc ctgttcgtcc gccgctttcc cgaacacgca tacggcgcgt tgctgtgcgc
      181 cggccttgcc aacggattgg gcagtatggt ttcctattgg atgggacgtt tgctgccttc
      241 ccgaaaaatg ccgtctgaaa aaacactgaa tctgatgcgg cgtttcggta tttggctgct
      301 tccgtttgcc tggctgcccg tcgtcggcga cgcactgccg cttgccgccg gctggctgcg
      361 gttgaatccg tggacaagcg ggctgatgct ggttatcggc aaaacggcgc gttacgcctt
      421 cattctgtgg ggaatgcaat attacgccgc ctgaaccgtg cgttataatg ccgaccgcac
      481 aaaccgactg cacaaaaaaa tgaacagaca catccgccaa gaaattttcg aacgcttccg
      541 cgccgccaac ccccatccga ccaccgagct gaatttcaac tcccctttcg agcttttaat
      601 tgccgttctg ctttcggcgc aggcgaccga tgtcggcgta aacaaggcga cggcgaagct
      661 gtttccggtt gccgatacgc cgcaggcgat gctggatttg ggtttggacg gcgtgatgga
      721 atacacgaaa accatcgggc tgtataaaac caagtccaag cacatcatgc aaacctgccg
      781 catcctgctg gaaaaataca acggcgaagt gccggaagac cgcgaggctt tggaatcgtt
      841 gcctggcgtg gggcgcaaaa cggcaaacgt ggtattgaac acagcgttcg gacaccccgt
      901 tatggcggtc gatacgcata ttttccgcgt gtccaaccga accaaaatcg cccccgggaa
      961 agatgtgcgc gaagtcgaag acaaactgat gcgcttcatt cctaaagaat ttctgatgga
     1021 cgcgcaccac tggctgattt tgcacggacg ctacacctgc aaggcactca aaccgcaatg
     1081 ccaaacctgc atcatcaacg atttgtgcga atatcccgcc aaagcctgac cgccgccgcc
     1141 cttcaaaatg aaacgcttga aaattcggac ggacgttgta gaatttcaac ctatgccgtc
     1201 tgaagccgac aaaatgcgct tcagacggca ttaaagccgg taccggcatc cgacaaagga
     1261 caaaacgtgt tcaaaaaata ccaatacctc gctttggcag cactgtgtgc cgcctcgctg
     1321 gcaggctgcg acaaagccgg cagctttttc ggtgcggaca aaaaagaagc atctttcgta
     1381 gaacgcatcg aacacaccaa agacgacggc agcgtcagta tgctgctgcc cgactttgtc
     1441 caactggttc aaagcgaagg cccggcagtc gtcaatattc aggcagcccc cgccccgcgc
     1501 acccaaaacg gcagcggcaa tgccgaaacc gattccgacc cgcttgccga cagcgacccg
     1561 ttctacgaat ttttcaaacg cctcgtcccg aatatgcccg aaatccccca agaagaagca
     1621 gatgacggcg gattgaactt cggttcgggc ttcatcatca gcaaagacgg ctatattctg
     1681 accaatacgc acgtcgttac cggcatgggc agtatcaaag tcctgctcaa cgacaagcgc
     1741 gaatataccg ccaaactcat cggttcggat gtccaatccg atgtcgccct tctgaaaatc
     1801 gacgcaacgg aagagctgcc cgtcgtcaaa atcggcaatc ccaaagattt gaaaccgggc
     1861 gaatgggtcg ccgccatcgg cgcgcccttc ggcttcgaca acagcgtgac cgccggcatc
     1921 gtgtccgcca aaggcagaag cctgcccaac gaaagctaca cacccttcat ccaaaccgac
     1981 gttgccatca atccgggcaa ctccggcggc ccgctgttca acctgaaagg acaggtcgtc
     2041 ggcatcaact cgcaaatata cagccgcagc ggcggattca tgggcatttc cttcgccatc
     2101 ccgattgacg ttgccatgaa tgtcgccgaa cagctgaaaa acaccggcaa agtccaacgc
     2161 ggacaactgg gcgtgattat tcaagaagta tcctacggtt tggcacaatc gttcggtttg
     2221 gacaaagccg gcggcgcact gattgccaaa atcctgcccg gcagccccgc agaacgtgcc
     2281 ggcctgcagg cgggcgacat cgtcctcagc ctcgacggcg gagaaatacg ttcttccggc
     2341 gaccttcccg ttatggtcgg cgccattacg ccgggaaaag aagtcagcct cggcgtatgg
     2401 cgcaaaggta aggaaatcac cgttgccgtc aaactgggca atgcttccga acaaaccggt
     2461 tcctcgtccg agccggacaa agccccttat gccgaacacc aatccggtac gttctcggtc
     2521 gaatccgcag gcattaccct tcagacacat accgacagca gcggcggacg gcttgtcgtc
     2581 gtgcgggttt cgggggcggc agaacgcgca ggcttgaggc gcggcgacga aatccttgcc
     2641 gtcgggcaag tccccgtcaa tgacgaagac ggtttccgca aagctatgga caaggcaggc
     2701 aaaaacgtcc ccctgctggt catgcgccgt ggcaacacgc tgttcatcgc attaaacctg
     2761 caataacaca cataatttaa ccaaaatatt ttttaaatga taaaatgccc gtcaataccg
     2821 attaacgggc attttacaga aatcagttgg ttgattttaa acaaatattt tttacgacaa
     2881 aaaatatttc cgcccatttt gccaacaaaa cccaaaccgg aggcggacag atacgctccc
     2941 atccggacgc ttaacacccc cgtaaaccat gcagctgatc gactattcac attcattttt
     3001 ctcggttgtg ccaccctttt tggcactggc acttgccgtc attacccgcc gcgtactgct
     3061 gtctttaggc atcggtattc tggtcggcgt tgcctttttg gtcggcggca accccgtcga
     3121 cggtctgaca cacctgaaag acatggtcgt cggcttggct tggtcagacg gcgattggtc
     3181 gctgggcaaa ccaaaaatct tggttttcct gatacttttg ggtattttta cttccctgct
     3241 gacctactcc ggcagcaatc aggcgtttgc cgactgggca aaacggcaca ttaaaaaccg
     3301 gcgcggcgcg aaaatgctga ccgcctgcct cgtgttcgta acctttatcg acgactattt
     3361 ccacagtctc gccgtcggtg cgattgcccg ccccgttacc gacaagttta aagtttcccg
     3421 cgccaaactc gcctacatcc tcgactccac tgccgcgcct atgtgcgtgc tgatgcccgt
     3481 ttcaagctgg ggcgcgtcga ttatcgccac gcttgccgga ctgctcgtta cctacaaaat
     3541 caccgaatac acgccgatgg ggacgtttgt cgccatgagc ctgatgaact attacgcact
     3601 gtttgccctg attatggtgt tcgtcgtcgc atggttctcc ttcgacatcg gctcgatggc
     3661 gcgtttcgaa caagccgcgt tgaacgaagc ccacgatgaa actgccgctt cagacgctac
     3721 caaaggtcgt gtttacgcac tgattattcc cgttttggcc ttaatcgcct caacggtttc
     3781 cgccatgatc tacaccggtg cacaggcaag cgaaaccttc agcattttgg gtgcatttga
     3841 aaatacggac gtgaacactt cgctggtatt cggcggcact tgcggcgtgc ttgccgtcgt
     3901 cctctgcacg ctcggcacga ttaaaaccgc cgattatccc aaagccgttt ggcagggtgc
     3961 gaaatccatg ttcggcgcaa tcgccatttt aatccttgcc tggctcatca gtacggttgt
     4021 cggcgaaatg cacacgggcg actacctctc cacgctggtt gcgggcaaca tccatcccgg
     4081 cttcctgccc gtcatccttt tcctgctcgc cagcgtgatg gcgtttgcca caggcacaag
     4141 ctgggggacg ttcggcatca tgctgccgat tgccgccgcc atggcggtca aagtcgaacc
     4201 cgcgctgatt atcccgtgta tgtccgcagt aatggcgggg gcggtatgcg gcgaccactg
     4261 ctcgcccatt tccgacacga ccatcctgtc gtccaccggc gcgcgctgca accacatcga
     4321 ccacgttacc tcgcaactgc cttacgcctt aaccgttgcc gccgccgccg catcgggcta
     4381 cctcgcattg ggtctgacaa aatccgcgct gttgggcttt ggcacgacag gcattgtatt
     4441 ggcggtgctg atttttctgt tgaaagataa aaaacgcgcc aacgcctgac ccctttccct
     4501 gttcagacgg catatgccgt ctgaagcttt ttggagcaaa ccgcaatgac cgtcccccat
     4561 attccgcgcg gccccgtcat ggcagacatt gccgccttcc gcctgaccgg agaagaaagg
     4621 caacgcctgc tcgaccccgc aatcggcggc atcatcctct tccgccgcaa cttccaaaac
     4681 atcgaacaac tcaaaacact caccgccgaa atcaaagccc tgcgcacacc cgaactcatt
     4741 atcgccgtcg atcacgaagg cggcagggtg caacgtttca tcgaaggctt cacccgcctg
     4801 cccgcaatga gtacgctcgg cgagatttgg gacaaagacg gcgcgtccgc cgccgaaacc
     4861 gccgccggac aagtcggccg ggttttggca accgagcttt ccgcctgcgg catcgatttg
     4921 tccttcacgc ccgtcttaga tttggactgg ggaaactgcc ccgtcatcgg caaccgcagt
     4981 ttccaccaca atcccgaagc ggtcgcccac ctcgccctcg cccttcaaaa aggtttggca
     5041 aaaggcggta tgaaatcctg cggcaaacat tttcccggac acggatttgt cgaaggagac
     5101 agccatctgg tcttgccgga agacaggcgc agcctgtccg aactcgaagc cgccgacctc
     5161 gcccccttcc acattatgag ccgcgaaggt atggcggcgg ttatgcccgc ccacgtcgtt
     5221 tatccacaag tggacacaaa gcccgcaggg ttttccgaaa tctggctcaa acaaattttg
     5281 cgccgcgaca tcgggtttaa aggcgtaatt ttctcggatg atttgactat ggagggcgcg
     5341 tgcggggcag gcggcatcaa agaacgcgcc cgcatttcct ttgaggcagg ttgcgacatc
     5401 gtcctcgtct gcaaccgtcc cgaccttgtg gacgaactgc gcgaagattt ccgcattcct
     5461 gacaatcccg ctttggcgca acgttggcaa tacatggcca acacgctcgg cagtgccgcc
     5521 gcgcaagccg ttatgcagac ggcggatttc caagaggcgc aagcctttgt tgccggcctt
     5581 gcctcgccgc aagacacggc gggcggcgta aaagtcggcg aagcctttta aatccgcccg
     5641 acacgctgca aaaaggcaaa acggtatagg cacgccgtat tttttgcgct ataatcacgc
     5701 cttacttaat tcggaatgta gcgcagcccg gtagcgcact tcgttcggga cgaaggggtc
     5761 ggaggttcga atcctctcat tccgaccaaa tccaaaccgc ctcggcatcc attgccggag
     5821 cggtttttcc ttttggcggc aacagaaaaa tgccgtctga aacaga
//
LOCUS       DAXHMX010000126         5801 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHMX010000126 DAXHMX010000000
VERSION     DAXHMX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.126"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..722
                     /locus_tag="ACFM4S_001716"
     CDS             <1..722
                     /locus_tag="ACFM4S_001716"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFC6051353.1"
                     /translation="QQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPE
                     KPAGVARYEDLITFVQDRPGHDVRYAIDTAKISRELGWKPQETFESGLHKTVQWYLDN
                     KTWWQNVLNGSYRLERLGTGK"
     gene            785..1651
                     /gene="rfbA"
                     /locus_tag="ACFM4S_001717"
     CDS             785..1651
                     /gene="rfbA"
                     /locus_tag="ACFM4S_001717"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6051354.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPTPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQMHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            1705..2262
                     /gene="rfbC"
                     /locus_tag="ACFM4S_001718"
     CDS             1705..2262
                     /gene="rfbC"
                     /locus_tag="ACFM4S_001718"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6051355.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDGWFKENIADCSF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFYVLSDTTELTYKCTDYYKPEAEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(2306..4324)
                     /locus_tag="ACFM4S_001719"
     CDS             complement(2306..4324)
                     /locus_tag="ACFM4S_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6051356.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(4520..5641)
                     /gene="dnaJ"
                     /locus_tag="ACFM4S_001720"
     CDS             complement(4520..5641)
                     /gene="dnaJ"
                     /locus_tag="ACFM4S_001720"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6051357.1"
                     /translation="MSNQDFYATLGVARTATDEEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYESCDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKAVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLNLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIIVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         1507 a         1671 c         1364 g         1259 t
ORIGIN      
        1 ggcaacaaat gccgtctgaa cagcacgaag ccttccgttt ccaccatatt tccaccgatg
       61 aagtctatgg cgatttaggc ggcacggacg atttgtttac cgaaaccgcg ccctacgcgc
      121 cgtccagccc ctactctgcc tctaaagcgt ccagcgacca cctcgtccgc gcgtggttgc
      181 gtacttacgg cttgccgacc attgtaacca actgctccaa caactacggt ccttaccatt
      241 ttccggaaaa actcattcct ttgatgattc tgaacgcgct tgacggcaaa ccgctgcctg
      301 tgtacggcga cggtatgcaa atccgcgact ggctgtttgt cgaagaccac gcgcgcgcac
      361 tgtatcaggt tgttaccgaa ggtgttgtcg gcgaaaccta caatatcggc ggccacaatg
      421 aaaaagccaa tattgaagtc gtcaaaacca tctgcgccct gctggaagaa ctcgctcccg
      481 aaaaaccggc cggtgtggcg cgttatgaag atttgattac tttcgtacaa gaccgccccg
      541 gccatgacgt acgctacgcc attgatacag ccaaaatcag tagggaacta ggctggaaac
      601 cgcaggaaac cttcgagtcc ggcctccaca aaaccgtgca atggtatctg gacaacaaaa
      661 cctggtggca aaacgtattg aacggcagct atcgtttgga acgcttaggt acaggcaaat
      721 agttttcaga cggcatcccg acgcaatgcc gtctgaaaac ccatcgcaaa ggaagaaaga
      781 aaagatgaaa ggcatcatac tggcaggcgg cagcggcacg cgcctctacc ccatcacgcg
      841 cggcgtatcc aaacagctcc tgcccgtgta cgacaaaccg atgatttatt accccttgtc
      901 ggttttgatg ctggcaggaa tccgcgatat tttggtgatt accgcgcctg aagacaacgc
      961 ctcttttaaa cgcctgcttg gcgacggcag cgatttcggc atttccatca gttatgccgt
     1021 gcaacccact ccggacggct tggcacaggc atttatcatc ggcgaagaat ttatcggcaa
     1081 cgacaatgtt tgcttggttt tgggcgacaa tattttttac ggtcagtcgt ttacgcaaac
     1141 attgaaacag gcggcagcgc aaatgcacgg cgcaaccgtg tttgcttatc aggtcaaaaa
     1201 ccccgaacgt ttcggcgtgg ttgaatttaa cgaaaacttc cgcgccgttt ccatcgaaga
     1261 aaaaccgcaa cggcccaaat ccgattgggc ggtaaccggc ttgtatttct acgacaaccg
     1321 cgccgtcgag ttcgccaaac agctcaaacc gtccgcacgc ggcgaattgg aaatttccga
     1381 cctcaaccaa atgtatttgg aagacggttc attgtccgtc caaatattag gacgcggttt
     1441 cgcatggttg gacaccggca cgcacgaaag cctgcacgaa gccgcttcat tcgtccaaac
     1501 cgtgcaaaat atccaaaacc tgcacatcgc ctgcctggaa gaaatcgctt ggcgcaacgg
     1561 ttggctttcc gatgaaaaac tggaagaatt ggcgcgcccg atggcgaaaa accaatacgg
     1621 ccaatatttg ctgcgcctgt tgaaaaaata atgtttgagg ccgtctgaaa cttttcagac
     1681 ggcctttaga tgaaagataa aaagatgaac atcattgata ccgccattcc tgacgtaaaa
     1741 ctgcttgagc cccaagtctt cggcgacgcg cgcggctttt ttatggaaac cttccgcgac
     1801 gggtggttca aagaaaatat tgccgactgt tcctttgttc aggaaaacca ctccaaatcc
     1861 ggcaaaggcg tattgcgcgg cctgcactac caaaccgaaa acacacaagg caaactcgta
     1921 cgcgtagttg tcggcgaagt gttcgacgtg gccgtcgata tgcgcgaagg ttcgccgact
     1981 ttcggcaaat gggcgggcgc aaccctgtcc gcgcaaaacc aatgccagct ttggataccc
     2041 gaaggcttcg cgcacgggtt ttacgttttg agcgacacga ccgaacttac ctacaaatgc
     2101 acggactatt acaagcctga agccgaacag gttttaatat ggaacgaccc ggcaatcggc
     2161 ataggctggc cgcttcaaac cgcgccgctg ctgtcgccca aagaccttgc cggcaaaacg
     2221 tgggcgcaag ccgaaaagct ccgccttaca ctttcccgat aaaaaatgcc gtctgaacgt
     2281 ttcagacggc attttctccg acaggctacc tacccgcctt cagtacgcgc tgtgcaaaga
     2341 aaaacatccc ggtaacgaag aacgccaaac ccaaccaaga agcggcggca tcccagtttt
     2401 gcagattcaa cgcgagcggc gcatccgagc cgccgttggt cacggagaag gcaataataa
     2461 acgccataat cacaccgatc aggctttcac cgacaatcag gccggcggag aacaaggttc
     2521 cgatgcgctc ggcgtttttc agacggcctt cacggttttc cgctttttta ccgatgatgt
     2581 gtttcaacac cgccgccaac accgcgcctg ccacgatggg catattgacg gacggcggca
     2641 gataaatacc cataccgacc gcaaggacgg gcagggcaag tttgccgcct gatgattttt
     2701 tcaacaccaa atcggcggca atcaacaccg cgccaatcgc gatgccggta aagatataga
     2761 cccattcgag gttgtgggcg aaaatgcccg acgcgatggt cgtcatcaaa gtcgcttgag
     2821 gggctgccaa agcctgcgcc gcgtccatac cttcgcgcgg cattgcgccg gtaaagccgt
     2881 aggcttcgta aagcagttcc aacacgggtg aaataaccag cgcaccgacg atacagccga
     2941 taatcagggc gacttgctgc cgccaaggcg tggctttgag caggtagccg gttttcaggt
     3001 cttgcaggtt gtcattggag atcgaagcca cgcagattac tgccgagccg caaaataaag
     3061 tcagtgccag caaaaatttg cggtttgcct catccgccat caaaccgccg gattcgccca
     3121 ccagcagcaa aaccagcgaa atcacgacga tggaaacgat gcccacgccg gaaatcgggc
     3181 tggaagacga gccgaccaaa cccgccatat aaccgcaggc ggcggcgacc aaaaagccga
     3241 tgacggaagc caaaagcgtg caaacgacca ccaaaagcca agccatgccg cccgtaatgt
     3301 gcgaatcgcc gataaagtgg taaaacgaca cgcctagaat aaacatcata gacaataccc
     3361 agaaaatcat ggctttaggc gacaaatcct gttcagcgcg ttctgtagcg ggcgcaccgc
     3421 cgccaaaact cttgaacgac atcttcatgc cttccaccat tggcttgagc agcatcaaaa
     3481 gcgtccaaac cgccgcgata cctatggttc ccgcgccgat gaagcgtact ttttccttcc
     3541 acagcttcat cgcaaacgcc gccatttcta tatcggaagg ctgcgggatg tgcgatgaaa
     3601 aatacggcac ggcaatgccc caagcaatcg aaatgcccaa cagaatggcg ataccgcccg
     3661 tcagtccgac caaatagccc gcgcccaaca atgccagtga aaagcccatc ggcagttgga
     3721 aaattgccgt accgctttta aaccaataac tcgcgctatc ggcaatcaca cgcagacctc
     3781 cggcgcaaaa gctcatcaat cccgccaacg caccgccggc cgccagctct ttgatgccgc
     3841 tgccgccctg acggttatcc ccttcttcat gaccgcccac tttcaaaatt tcagcagccg
     3901 ccacaccttc cggataaggc aaatcgcttt tcaccaccat cgcgtaacgc agcggaatgg
     3961 tgaaaatcac ccccaaaatc ccgccggcaa tacataaaag cgtcgtctgc cagaacggga
     4021 aaccgctcca gtagcccgcc atcagcaaac cgggcaggac gaagatgatg gttgaaagcg
     4081 tacccgcagc cgaggcttgg gtctgcacca tgttgttttc caaaatattg ctgcctttga
     4141 aaaactttaa aaccgccatc gaaatcaccg ccgccggaat cgacgaggca aaggtcagcc
     4201 cgactttcaa accgaggtaa acgttggacg cagtaaaaat tacagtgatc aatgcaccga
     4261 gtatcatgcc tcggagcgtc agttcgcggt attcttctac cgaaccggat aaagatttat
     4321 tcattattct tcctttgaca acagattccc gcatattgtt ggcaatatgt cataatgtca
     4381 agttttaaaa agaacagtta aaaacagtta tccaatccta tctcgtactc ccttgaaaat
     4441 aaaaactatt ttcaagcaaa caaaaacagt cgtatcaggg agattccccg atacggccgc
     4501 ctgtttacaa gcctcaaaat caatcaaaca aatcgcgcag cttgtctaaa aacgatttct
     4561 tacgcggtgt ttgattttcc aacccggtag aaatccgttc aaattcttct aaaagctctt
     4621 tttgacggtc ggtcaaatta acaggtgttt cgacgataat atggcagtac aaatcgccgg
     4681 tcgcgctgct gcgtaaagat ttgactccct tacccttcac gcgcatcctc ctgccggttt
     4741 gggtttcttt ggggacggtg agcttgactt tgccgtccaa ggtcggcact tccaactctc
     4801 cgcccagagc cgccgtggca aaactgatcg gcagttcgca atgcaaattc aagccgtcgc
     4861 gttggaaaat cttatgcgcc cggatgcgga cggttacata caaatcgcct gcgggcgcgc
     4921 cgtgcatacc cggcccgcct tcgccgctca aacggatacg ctgcccgtca tcgataccgg
     4981 cgggaatatt gacttccacc gccttgacgg ccttattccg ccccgcgcca cggcatttga
     5041 cgcaaggttc tttaatgtgt ttgccggcac cgtggcaggt cggacaagtc tgctgcatac
     5101 ggaaaatcgc ctgctggata tgcaccgttc ccgaaccttt gcaagtcggg caggtttccg
     5161 gggatgtccc cggtttcgca ccgctgccgt tgcagacatc gcacgattca taagtcggaa
     5221 tgttgatgcg tttcttcacg ccttttgctg cttcttcaag cgtgatttcg ataccgactt
     5281 gaacgtcctc gccctgataa tcaggctggg cgcgccccga accgcctcca aacatttggc
     5341 tgaaaatatc cccaaagtca aaaccttgag caccgccgaa tcctccaaac cctccgaagc
     5401 ccccctgtcc gccgccttca aacgccgcat ggccatactg gtcgtacata gcgcgctttt
     5461 ctttatcgga caaagtttca tacgcctttt gtacttcttt aaacttctct tccgcctctt
     5521 tattgtcagg attgcggtcg ggatggtatt tcatcgccaa tttacggtag gcttttttaa
     5581 tttcttcatc ggtagctgtt cttgccacac ctagggtggc ataaaaatct tgattgctca
     5641 tcttttcatc tgattcaaaa taaaatcgtc gataaaataa gggcaacagg acaaaacaca
     5701 aggtttcccg aacaaaaacc gcctttcaga cggcatttct tctgttgttc ctttaacaat
     5761 gcgccgggta tcggcggctt tacccgtccg gaaattgaaa a
//
LOCUS       DAXHMX010000127         5792 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHMX010000127 DAXHMX010000000
VERSION     DAXHMX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.127"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            131..565
                     /locus_tag="ACFM4S_001721"
     CDS             131..565
                     /locus_tag="ACFM4S_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6051358.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="ACFM4S_001722"
     CDS             complement(578..1441)
                     /locus_tag="ACFM4S_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6051359.1"
                     /translation="MELNEFFDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="ACFM4S_001723"
     CDS             complement(1449..2066)
                     /locus_tag="ACFM4S_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6051360.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2843)
                     /locus_tag="ACFM4S_001724"
     CDS             complement(2229..2843)
                     /locus_tag="ACFM4S_001724"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC6051361.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQIDLQEG"
     gene            3049..4821
                     /locus_tag="ACFM4S_001725"
     CDS             3049..4821
                     /locus_tag="ACFM4S_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6051362.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFSDGISLTGDDRTLLQTLIRQLGVSLASAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4818..5474
                     /locus_tag="ACFM4S_001726"
     CDS             4818..5474
                     /locus_tag="ACFM4S_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6051363.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLNMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT         1434 a         1634 c         1335 g         1389 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gtcagctttc ccacgccgcc acggcttata tccccccgaa
      241 cgattttcaa ccgaactgcg acatacgccg actcgggctg acccaaagtc agcacaatga
      301 gctgcgtaaa atccgcaccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcttcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accgcttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ccccccgcat ccccgtcgta ccggcattct cgtcaaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtaaacc aaaacgttgg cgcaacgttg agacagaccg ccgtctaacg
      961 cggttttcaa tgccttatag gtttcatcgc cgctttcaaa cgacaaatcg tcgcaaaata
     1021 cgataaattt ttccggacgt tccttcaaaa gcgtcaacag gtaaggcagg ccgattaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgt agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacattg ttcgcgggtc
     1201 tgccgacaat gaactgttcg gtattacgca ccagcaattc ggtttgcctg ccgactcccg
     1261 ccagccttac caagggaaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gaaaaattcg ttcaattcca
     1441 tctctacctc actttgcata tctttgcgcc atcagccgtt cgacggtgtc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatc ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcagatgg acattgaaac ggccgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcatgcggga gcgaaaacca aaccgtgcgg ttgaacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccccaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tattggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtgtg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aatccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtatggc ttgacctttc ctttccacgc cggtcggcgg
     2221 tcttgccctt atccctcctg caaatcgatt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccgaccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa acctcgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cagcagtcgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgcgcatcg ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggcg atatggctga cctgcggcct gaccctgtcg
     2701 atgacatggt ctatcagtgc cttacccccc aaaagagcaa gccccttgtc ctcgcctccc
     2761 atacggctcg cctgaccgtc ggccagtatc agggcaaaag ttttcattgc ggatgttctc
     2821 ttggaaaagt tcgaggtttt catgattgca gtctgccgtt cccaatgact caaaatgccg
     2881 tctgaagcag atggcaaata aattcatatt atctgaattt tatcataaca tgatttaaca
     2941 ctgaagcggt gcggattaag ttttccttaa ccaattcttt ctgagcagtt gtatctaatc
     3001 ccaaagaatg atattgtttg cattatttag aacaattttt cgccgagcat gatactgcca
     3061 gcccgttttt cagacggcat cagcctttcc ctacgcctga aactcctgac cggagtgtgg
     3121 gtcgggttgg cggcattgtc tgtcgttttg acactgctgc tctctttgcg tctggaaaac
     3181 gcggcctccg tcatcgaaga ggcgggcaac ttgagaatgc aggcataccg tctggcatac
     3241 atggcgggtg aaggatctcc ccgctcccaa atagacaatc aggttgacga atttgaaaaa
     3301 agcctgaatc gtatttcaca aagtgatgcc atccatccgc tgattccttc ggacacccct
     3361 cttgcttatg atttgataca atccatgctg attatagatt ggcaggcaca catcctcccc
     3421 ccgctccagt cctaccggcg accgactcag gtcgatctct accgctttgc cggaaacatc
     3481 gaactgtttt tgcaggcatt ggaaaatgcc aacgaaaaaa acacatggtg gctcaggcgt
     3541 tttcaatggg caattatgtt gatgacgctg gtgtcgtctg tactgatgct gttttggcac
     3601 cagatttggg ttatccggcc gctgcaggcg ttaagggaag gtgcggaacg catcggacgg
     3661 aggtgtttcg atattccggt tcccgaaggc ggtacgccgg aattcaaaca ggtcgggcgt
     3721 tgtttcaatc aaatgggcgg caggttgaaa attttatatg atgatttgga aggacaagtc
     3781 gccgagcaga cacgcagtct cgaaaaacaa aatcaaaacc tgaccctgct gtaccaaact
     3841 acacgggacc tgcaccaatc ctacataccg caacaggctg cagaacattt tctaaaccgt
     3901 atcctgcccg ccgtaggagc agattccggc agagtttgtt tggacggcgg atccgatgtt
     3961 tatgtttcca ttcatcatgc ggattgcggc acagcagctt cggatttggg gaagtaccat
     4021 gaggaaatct tccccattga gtaccagaac gaaacattgg gcaggctgtt gctcagcttt
     4081 tcagacggca tttccctcac cggagacgac cgcacgctgc ttcaaaccct gattcggcaa
     4141 ttgggcgtat cgctcgctag cgcgaagcag gaagaagaaa aacgcctgct tgcagtattg
     4201 caggaacgca acctgattgc gcaaggatta catgacagca tcgcacaagc attaacgttc
     4261 ctaaacctac aggtacagat gctggaaacc gcctttgccg aaaacaaacg ggaggaagcc
     4321 gcagaaaaca tcagcttcat caaaacaggc gtgcaggaat gttatgaaga tgtccgcgaa
     4381 ctgctgctca acttccgtac caaaatcagc aataaagaat ttcccgaagc cgttgccgac
     4441 ctattcgccc gctttacgca acaaaccggg ataacggtcg aaaccgcctg ggaaaacggt
     4501 tcgttcctgc cgcctcagga agcgcagctc caaatgattt ttatcctgca ggaaagcctg
     4561 tccaacatcc gcaaacacgc ccgcgccacc catgtaaaat tcaccctttc cgaacacggc
     4621 ggacgcttta ccatgaccat ccaagacaac ggacaaggtt tcgacacgga gaaaatagga
     4681 gaacccacgg gcagccatgt cggactgcac atcatgcagg agcgtgccaa acgcatccat
     4741 gccgttttag aaatccgttc ccaagctcaa cagggaacca ccgtctcatt gacggttgca
     4801 tctgaagaaa gcttgaaatg actattaaaa ttattctgat agacgaccat accctcttcc
     4861 gcagcggcat taaagccctt ttgtcgcgcc aacacggttt tgaagtcatc ggcgaagccg
     4921 cagacggcct ctcgggcatc aaaatgatta gtcggctgca acccgatgtc gtcctgctcg
     4981 atttgaatat gcccggcatg aacggacgcg aagcactctc ccaaatcatc agcatcaatc
     5041 cgcagcaggc agtgattatg ctgaccgttt ccgaagacag cgacgacttg accgaatgta
     5101 ttcgcatcgg cgcgcgcggt tacctgctga aaaacatcaa cgctgacttt ctgctcgaaa
     5161 gcatacgcaa agccgccgaa ggcgataatg tattctcgcc cgagatgacc gccaaactcg
     5221 ttaaaagcct gatttccccc caacctgccc aagggacaca ggcactctcc tcacttaccc
     5281 ctcgcgaact ggaaatcttg ggctatctcg ccgcaggaca cagcaacaaa atcatcgccc
     5341 gccacctcga tcttgccgaa tccaccgtca aagtccacgt tcaaaacctg ctccgcaaac
     5401 tcaacctcag cagccgggtg caggccgccg tttacgccat ccggcacaac gtcccccaac
     5461 ctgtgccgga ataaggcggg ttcagacggc atattagggg ttttaatccc cgtacggtca
     5521 ttcggataac agaccaagca tgtaagttta tgcccccata agtacgcttg gcatagcagt
     5581 aatattgttc ggtttagtgt tttccgtttg cccctatctg atactgcaat atcaactatg
     5641 ccgtctgaaa acgcatcatc atgatatttt cagacggcat aataaaaagc ggaaatacta
     5701 atgcagggta aaatgttcca ttccgaatcc cataaatata caatggctta tatcgtttaa
     5761 gcatgtgtaa cccaccctca tatcaatcaa ta
//
LOCUS       DAXHMX010000128         5789 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHMX010000128 DAXHMX010000000
VERSION     DAXHMX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.128"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(25..246)
                     /locus_tag="ACFM4S_001727"
                     /pseudo
     CDS             complement(25..246)
                     /locus_tag="ACFM4S_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase TauD"
     gene            complement(245..352)
                     /locus_tag="ACFM4S_001728"
                     /pseudo
     CDS             complement(245..352)
                     /locus_tag="ACFM4S_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="ACFM4S_001729"
     CDS             complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="ACFM4S_001729"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6051364.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1150..1941)
                     /gene="recO"
                     /locus_tag="ACFM4S_001730"
     CDS             complement(1150..1941)
                     /gene="recO"
                     /locus_tag="ACFM4S_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6051365.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVSFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTDALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(1975..3102)
                     /gene="pheA"
                     /locus_tag="ACFM4S_001731"
     CDS             complement(1975..3102)
                     /gene="pheA"
                     /locus_tag="ACFM4S_001731"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6051366.1"
                     /translation="MLECTANHRSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3141..4373)
                     /locus_tag="ACFM4S_001732"
     CDS             complement(3141..4373)
                     /locus_tag="ACFM4S_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6051367.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(4892..5746)
                     /locus_tag="ACFM4S_001733"
     CDS             complement(4892..5746)
                     /locus_tag="ACFM4S_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6051368.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWNVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         1347 a         1664 c         1581 g         1197 t
ORIGIN      
        1 ctatattaca taatcatcgc ctcaatattt aatatcccaa aatatagatt taaaaccatc
       61 gcctttgtgg tttgtcagca ttcctaattg agatagaaag tcaaataatt tcttgtttct
      121 ccggctgcca tcaagatggt ttagattaag accgcgaaca atacaataac caatacgctg
      181 tactgtattg tgaatttctt ctatcaaact attattgttt gcagataaaa tatacttttg
      241 attcatatag tgaattaaat ttaaaccagt acggcgttgg cttgccttgc cgtactattt
      301 gtactgtctg cggcttcgcc gccttgtcct gatttaaatt taatccacta taacatatcc
      361 ctgtcgtgaa atgctgtctg aaagggtttg cctgccctta cggcagcagc cttgccctga
      421 acatcgcctc cttcatctgg cgcacggctt cgggcagtcc gaggaagagg gcttgggcaa
      481 tcagcgaatg cccgatgttc agttcgcgga tggcgaggat ttgggcgatg ggggtaacgt
      541 tgtgtatggt cagtccgtgt ccggcgttga cgaccaagcc caaatcgccg gcaaaatgcg
      601 cgccgttttg cagacgctcg aattgcttga gttgttcggc gtggctgcgc gcgtcggcat
      661 acgcgccggt gtgcagctcg acaacgggcg cgccgacatc ataggcggct tggatttgcc
      721 tgtcgtcggc atcgataaag agagagactc ggatgcctgc gtcggtcagg attttggtga
      781 acccggcgat tttttcctgt tgcgccaata cgtccaagcc gccttcggtc gtgatttcct
      841 gacgtttttc aggcacgatg cacacgtctt ccggcatcac tttaagcgcg ttttcgagca
      901 tttcttccgt caacgccatt tcaaggttca ggcgcgtgcg gatggcgttt ttgacggcaa
      961 acacatccgc gtctttgatg tggcggcggt cttcgcgcag gtgcatggta atcaaatccg
     1021 caccgtgcgt ttcggcaacc agtgccgcct ccacggggct gggataagtc gtgccgcgcg
     1081 cattgcggac ggtagcgatg tggtcgatgt tgacacctaa aagcataatc tttcctttta
     1141 tttctgcctt cagacggcat ttgaagccgt gccgtccgaa gtcgggacgg tttcccgggc
     1201 ggtttctttg cggtcaaact gccgtatctg ttccaacacc tgccgcgatt tcagcccctc
     1261 gggcaacagg tggcggataa aaagccgtgt gattttcaat gcctgttgca ggctttcggc
     1321 ggtgcggaaa ctgccttcgc gcaaatcgat caggctttgc ccttcgacaa ttgcgccggc
     1381 ctcgtgcgaa agcgcgtccg tgtcccggca tacgggcatg accgcctctt cgggcatcag
     1441 ccggtaggtt ttatccgcca aaatatcgcc gcccgtgccg tcggcgtgca aatcgggggc
     1501 aacgcccaag gcattgagca gcttccactc gaagcggcgc aaatcggcaa tgtggttggc
     1561 ttcacgacac accgcctcca tcacttttgc caacgcgtcg tacagttcgg gcatcgggtc
     1621 ttcgcgcgcg gtcagtttca acaccagttc gttcacatac agaccgctga acaacgccct
     1681 gccctgcggc tgccgccatc cgccgaccca ttcggcacgg tgcagggttt tgagttcctg
     1741 actgccgtac cacgacgcac tgacgggcac gaaggaaacc agcacgccgc gcaattcgct
     1801 ctgccgcttg cgcgcgcttc ttgccaaaag tgccacacgc ccgtaacggc ggctgaacgc
     1861 ttcgacccgc aggctgcttt cgcgccatgg cgaggaagcc aacaggaaga caggttcgtg
     1921 gttgactcgg tgttcggaca tcatgcctga aatgccgtct gaacgctgct gccgctacaa
     1981 aacggcggtc gggtacgaac cgatgacttt gacgaacgaa gcgcgttcgc ccaagcgttc
     2041 caatgccgtc tgaatctgcg cgtcccggcg gtgtccttcg atgtcgatga agaacaggta
     2101 ttcccacaaa acggatttac tcggacggct ctcaaacttg gtcatggaaa tacccgactc
     2161 agtcagcggt tgcagcagcg aggcaaccgc gcctgcccgg ttgggcgcgg aaacggccag
     2221 cgaagtcttg tcgctgccgc ttgcaccggt ttcgtgatgt cccatcacca agaagcgcgt
     2281 ggtgttgttc ggttcgtctt cgatgcactc ggcaaccata tcgagtccgt agatttccgc
     2341 cgccgtgcgt ccggcgatgg cggcaaccgt accgtcgtcc gattcggcaa ccagccttgc
     2401 ggcttcggca ttgctggaca cggcaatccg ttcggcgttg ggcaggtgtc tgcccaacca
     2461 gtcgttgcac tgcgccaacg cctgcgcgtg ggaaaagact ttggcaatgc cttcggtgct
     2521 gccgttgttt ttacgcaaaa ggttgtggtg gatgcgcaaa acgacttcgc cgcacgcctg
     2581 caacgcggta acggcaagca aatccaacgt gcgcccgacc gagccttcgg tcgagttttc
     2641 cacgggcgcg accagataat ccgcctgacg ggtttcaacc tgcttgaagc agttgtctat
     2701 ggtcggacac gccatggtgt gcgcggcgtg tccgaaatgc ttgatcgccg cctgctgggt
     2761 aaacgtgccc tgcggcccca gataggcgat ggtcagcggg cgttcgacgg cgaggcactc
     2821 gctcatcact tcccgaaaca ggcgtgctac cgattcgtcg ggcagcgggc ctttgttcaa
     2881 atcctgaatg cggcgcaaca cggcgacttc gcgttcggga cggtacactg cgcccgtgcc
     2941 tttcagctcg ccgatggcgt gggcgtgttg cgcacgttcg ttgagcaggc gcaggatttc
     3001 ggcatcgatg gtgtcgatgg cgttgcggtg gggaaggagg agttcgtcga tagtttggga
     3061 cataatttcg cctgaacggt ggtttgccgt gcattctaac atcaaatgcc gtctgaaacg
     3121 gtttcagacg gcatttgatg ttaaaggtat tcgctctgtt cgttttcttt ccacgcacga
     3181 tgtgagcaga gccacagaag cgcaatgccg caagaggtgg acgcggtcat cgttgccgcc
     3241 atcacggttg ccgaaccgtc gtgcaagaag gtcgccgcca tacccacccc cgcgccgatt
     3301 aaagattgga atacacccaa tacggcgttt gcgctgccgc cctcttcttt gaaataagac
     3361 ataaagcatg cctgcgtgtt cgcaccgacc aagccctgcg taccgacgga aaacatcacg
     3421 cacgcgacca gcagccaaaa cgggggcaac ccgaaaaaca gcacggcggc gagttgggac
     3481 aggttggcgg caaactggac gacaatcccc cacagcagga tgctttgcgg atgcacgccg
     3541 gttttgagcc gccacgcggt aacgcggttg aaaaacatca tcgtgatgat gttgagtgca
     3601 aacgcccaag cgtattgatg aggcgtaaca cggtagagct gctggtacac gaaggaagat
     3661 tcggtcagaa aggcgaacat cgaaccgaag ctgaatgcct gaaaaaacag ataacccatc
     3721 gcggcacggg ttttcaatac gcgtttgaac cgcccagcca ccagcccgaa cacatcccgt
     3781 ccgattttgc cgccgacggc aggcttgggc aggaaatact gtaccaaacc gagcagcacc
     3841 agcgaatacg ccgccagaaa aacaaaaatc gcctgccagc cacccaagcc ctgcaacaat
     3901 gcgccgacca tgggtgcgac cagcggcaca accatcaaaa tgatgccgat aagggcaaac
     3961 atctgggcgg ctttgcgtcc ggaataataa tcgcgcacca ttgcgccgac gatgaccaca
     4021 gtcatgcccg caccgaatgc ctgcacgacg cgcaggttga ggagctgttc ggcactcgaa
     4081 gcaaatacga tggcggcaac ggcaaggcag tagacggcca gtccggtcag cgcgacgggt
     4141 ttgcgccctt tgatgtcgga caccgaaccg ccgaccacct gtccgaacgc cgtgccgaac
     4201 ataaacaaac tcaggctctg ttcgatgcgg tggacatccg cgttcagcga ctgcgccatt
     4261 tcgggaatcg cgggcaggta ggcatcgatg gaaaacggca tcagcgtaac cagcatcgcc
     4321 atcaaaaccg ccatcagttt ttcgctcatt tcaggataat gggcagaagg cataaattct
     4381 cgtccgattc gcaaacaaac cgcccggcgg cggaaaacac aatacgcgga aacggaaaat
     4441 tttaacacac cggcacaaac aggcggcaac aaaagccccg gcaaaaccgg acgaaccgga
     4501 acgatatagt ggattaacaa aaaccagtac agcgttgcct tgccttagct caaagagaac
     4561 gattctctaa ggtgcttaag caccaagtga atcggttccg tactatctgt actgtctgcg
     4621 gctcgccgcc ttgtcctgat ttttgttaat ccactataaa atcccgtcat tcccgcgcag
     4681 gcgggaatct ggacatccaa cgctgcagca atttgtcgga aataaccgaa actcaaaaaa
     4741 ccggatgccc gcctgcgcgg gaatgacgat gcaaagatgg tcgttatttc ggataaatat
     4801 ccgtcttttc gttccggata cccgctttcg caggaatgga gatggaaaga tttcagccta
     4861 ttcggcaatc atcaaatacc gcttcccaac tttatttttg atgcgacagc cggccggcaa
     4921 ccaaggtggc ggcgatgacg acaaacacgc ccaaggcgga cacgggcgac aggtgttcgc
     4981 ccaaaatcaa aaccgccagc agcacgccga cgacgggttc gagcgaaatc aacagtcccg
     5041 aaacgttggc aggaacacgg ctcatcccct tgttccacag ccaataggcg taccagccgc
     5101 accccaagcc caaatacagc agcgacaata ccatcccgac gttccagtcc acggtataac
     5161 tttgcgccaa agcaagcgaa aacggcaggc acatcaacga tgcggcggca atggaaacag
     5221 atgtgaatgc cggtgcgccg atgcgtgcaa tcagcctttg cgtcggacgc atagcggcac
     5281 aaaagcccgc gcccgccaac aacaccagca ggcagccgaa ccagccgact tcgccgccct
     5341 cttccgcacc gcccgccatc agcagcgcga caccggcaaa tgccgccgcg ccgcatatcc
     5401 agtggtaggc acgcgctttg tcgttgaaga aaaagtgtcc gacaaacacc atcagcagtg
     5461 gctcgagtcc gacaatgacc gatgcgctgg cggcggaagt gtatttcaac ccgacaaact
     5521 gaagcagcag ggtcagcaca tagttgacga acgacacaat cagcaacggc ttccattcct
     5581 cacgcggaat cttgccgaca tgacggcggc aggcgggcag tgcaggcagc gcagcaatca
     5641 gcaggcgcac gccgaccatc aatgcgggat cgatgccgcc atagacatat ttggcggcaa
     5701 taaacgagct gctccagata atcagggcaa ggatttggta aaacatagtg agtgataaag
     5761 tcagatggtc atacaaaagt atagcggat
//
LOCUS       DAXHMX010000129         5728 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHMX010000129 DAXHMX010000000
VERSION     DAXHMX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.129"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..160)
                     /locus_tag="ACFM4S_001734"
     CDS             complement(<1..160)
                     /locus_tag="ACFM4S_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6051369.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGD"
     gene            complement(200..1162)
                     /gene="mafA"
                     /locus_tag="ACFM4S_001735"
     CDS             complement(200..1162)
                     /gene="mafA"
                     /locus_tag="ACFM4S_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6051370.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(1346..2065)
                     /gene="pyrH"
                     /locus_tag="ACFM4S_001736"
     CDS             complement(1346..2065)
                     /gene="pyrH"
                     /locus_tag="ACFM4S_001736"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6051371.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2284..3138)
                     /gene="tsf"
                     /locus_tag="ACFM4S_001737"
     CDS             complement(2284..3138)
                     /gene="tsf"
                     /locus_tag="ACFM4S_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6051372.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(3267..3995)
                     /gene="rpsB"
                     /locus_tag="ACFM4S_001738"
     CDS             complement(3267..3995)
                     /gene="rpsB"
                     /locus_tag="ACFM4S_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6051373.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAIRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4172..4405)
                     /locus_tag="ACFM4S_001739"
     CDS             complement(4172..4405)
                     /locus_tag="ACFM4S_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051374.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4483..5064
                     /locus_tag="ACFM4S_001740"
     CDS             4483..5064
                     /locus_tag="ACFM4S_001740"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6051375.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            5125..5715
                     /locus_tag="ACFM4S_001741"
     CDS             5125..5715
                     /locus_tag="ACFM4S_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC6051376.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1281 a         1307 c         1582 g         1558 t
ORIGIN      
        1 catcaccgaa cagatggtat ttgccacccg gttcgtagtg ttttgcctgc atatcatcgc
       61 gcaaacgggc atccaaaccg ttcgcatgac taatggggat ttgcagcaac gatattgctg
      121 ctgcaaacag catcatgaat ttttgaatag gcaaattcat tttgttttcc ttggtagcaa
      181 gcggttatgg tagtgtgaat caaggttgcc cttgtctatg ttgtcgcact acttcatcgc
      241 tgtatccata cccctcatga ctgttatcag tctctacgga tggggcggag ttacccgtat
      301 gattgccgta tggtcggata tcggagaaat cgaccattaa tccttccgtc ggtttaattc
      361 ctttgcttac tttatacggc cccatccaca atgcgtaatt ttctttatag gcagcttcaa
      421 acgcattggt tttgggtttg atgagcaatt ttttattggt tctgtctact gcgaaatatt
      481 ccagttttgt ttgggctttc agtgtttcgg cattgtatag gtgcatttcg gttctgttgc
      541 gtatcgttcc gaatacgtcg atgttaataa acacatctgt atcggcattg gcaggagaaa
      601 caacgtctat gccgcgcagg aaaaatacgg tctgtaccaa gtgggaaaga aaggcagtgt
      661 cgcgcgggtt agtcgtcaag gtttcatttc gataatcccc catcccgcca atatttaagc
      721 ccagactgct cctacttccg ctaccgtctg attgggtgcg cgagcgtgca ggggcattaa
      781 gtgtagataa agaagtggtt aaacctgtca aaccgcctga tgttgtttca gcggtggttt
      841 cgtaacgtgg ataggtgtaa tcggtacgga cggcagggct gtttatgtat tcgccacgaa
      901 tcagtgcatc aatggagtag cgaccccctg tcaaactgcc tgaaccttgg tcgcccatag
      961 ttgcaatgta caatgcaact tttcgtccgt gtaatgcctg taaatccatg tctttaacgg
     1021 cagctctggc agaagcggcc acaagttctt gttcgaccgc aaagcgttta cctccgccat
     1081 gcgatggaat acctgtcagt gtcccgcagg cggataaaat aaaaactgaa aaaagaatag
     1141 gtatcagcag ccgtgcttgc atagattttc tcctttgatg aaaaacaaat tgtatcaaaa
     1201 ttgtaaatat agtggattaa atttaaacca gtacggcaag gcgaggcaac gccgtactgg
     1261 tttaaattta atccactata atctccatat aataaatggc tgtctgaagc ccatatgcga
     1321 ctatatctgc cgacactatg gtcaatcagc agtgaaccag cgtaccttcg ttttcaccgg
     1381 ttactacacg tttgagcgag ccttctttag caatgccgaa tactacaata ttgagcttgc
     1441 gttcgcggca gagggcgaaa gcggtggcat ccatcacttt gaggtttttc aacaaggctt
     1501 cgtcaaaagt aatggtttcg tagcgcgtgg cggatgagtc ttttttgggg tctgcggtgt
     1561 acacgccgtc aacgttggtg gctttgagca tcacgtcgca gttcatttcc gcaccgcgca
     1621 atgcggcggc agtgtcggtc gtgaagaacg ggttaccggt accggcggca aaaatcacga
     1681 ctttgccttc ttccaaatat tggatggctt taggacgggc gtaagtttca gcgatttgct
     1741 gcatagacag tgcggattgt acgcgcgctt tgatgcctaa agtttcaaat gcgtctttga
     1801 gtgccaacgc gttcatcacg gtcgccatca tgcccatgta gtcggcggtg gcgcgatcca
     1861 tgctgcctgc ttgggcggat acgccccgga aaatattgcc gccgccgaca acaataccga
     1921 cttgcacgcc cattttaacg acttcggcaa tttcgccgac agtttgaacg atggtatcgt
     1981 gattgatgcc gaacggatcg gaacccatca gggattcgcc ggagagtttc agtaatacgc
     2041 gtttgtattt gatttgctgt gtcatgggat accttgcttt cttgaatgtc gtcggtcgga
     2101 gtttgggccg tctgaatttc aaacggtcgg aatattaatg atagggtgtg tcggtttatt
     2161 attgttttgt tttcagacgg cctttatatt cggtcgtctt aaaataggta ccgtaaacgg
     2221 ttttctcaaa aaaaagcacc ctgattcgtt tggaatccag gtgctttctt tttcataagt
     2281 gccttacact ttggcggctg cggctacttc ggctgcgtag tcgacaacgg ctttttcgat
     2341 gccatcgcct actttgtagc gtacgaagct gaccacttca gtgccgtttt ctttggcgaa
     2401 ttgggcaaca gtttgatcag ggttcatcac gaatgcttgg ccgttcagag tgatttcagc
     2461 caagaatttg cggatacggc cttcaaccat tttagcggcg atgtcggcag gtttgccgga
     2521 agcgatggct tgttcggtgt agatgtggcg ttctttttca acggtttcag catctacttc
     2581 ggcttcgctt acgcattgtg gtttggcggc aacaatgtgc ataccgattt tgcgtgctac
     2641 gtcttcagag cctttgtact caaccaatac gccttcggtc gccaatgcgc cgtggatgta
     2701 ggcaaccagt tggttggcag tgtcgatcac ttggaagcga cggacagaca tgttctcgcc
     2761 caatttggcg atgatggctt tgcgttctgc ttcaaccagt tcgctcagtt cttcaacaga
     2821 agccggtttt ttctcggcag cagttttcgc aacgaagttg gcaaattcta cgaagcccgc
     2881 gtctttagca acgaagtcgg tttcgcagtt tacttcaacc aatgcgccga cattgccgtt
     2941 gattgcataa gccaatacgc cttcggcagc ggtacggccg gccagtttac cggctttcgc
     3001 accggatttg atacgcagga tttcttcggc tttgtcgaag ttgccttcgg cttcaaccaa
     3061 ggcttttttg cattccatca tgcccaggcc ggtagcggcg cgcagatcgg caaccatttt
     3121 tgcagtaatt tctgccattt tgaatctcct agatttttgg gaaatacgga aaggattttc
     3181 cgcattcggg aatcgtgtgc cgcgaacgga cgttcagacg gcatatttga gaaaaggggc
     3241 ataacgcccc tcttcggttt gccagattac tcggcagcgg cttcttgggc agcggctacg
     3301 gtttcttgca gcgcttggtt tttgccttcc aaaactgcgt cagcgatgcc gcggcagtac
     3361 aggcggatgg ctttggcgga gtcgtcgttg ccggggatga cgtatttcac gccgtcaggg
     3421 ctgttgttgg tatcgactac ggcgataacg gggatgccca gtttttcagc ctcgaccaga
     3481 gtaccttttt ggtagccggt atcgataacg aaaatcgcgt cgggcaggcc tttcatgttt
     3541 ttgataccgc ccaaagaacg ttccagtttt tcaacatcgc gttgcatttc cagaatttct
     3601 tttttgctga aaccgctttc ggcagcattt tccaaggctg cggttttttc ttccaggcgt
     3661 ttgatggatt gcttaacggt tttgtagttg gtcagcatac cgcccaacca gcggtaatca
     3721 acgaaaggca taccggcgcg ggtcgcttct tcgcggatga tgtcgcgggc ttggcgtttg
     3781 gtacctacga acaatactgt acctttgttg gcaaccaaac gacgtatggc ttcttgcgcg
     3841 tcttggaaca tcggcagggt tttttccagg ttgacgatat ggattttgtt gcgcgcaccg
     3901 aaaatgtatt gtgccatttt cgggttccag aaacgggttt ggtggccgaa gtggacaccg
     3961 gcttcaatca tctgacgcat agtaatttga gacattttga tttccttgaa agggttaagg
     4021 gcacacattc aatcgcatag aacttgatag gcgacaccta ttgtcaagca cccttcggcg
     4081 aaagtgggca taagttttaa aaaataaaag agtgtttccg aaatggaaaa ctgacggatt
     4141 atagcggata agaggccgtc tgaaaaggga ttcagggttt tgtgccgctg tctttttgcc
     4201 gttgtgcacg ctgctgttcg aattctgcct gttcctgttg cttcatcaag cggttgcggc
     4261 ggcggacggt tacgacgaag accgtgaaca cggtgggcag caccgcccaa aaaaccaaat
     4321 aaatcaaggc gcgcgcaata ctcggctgcg cggcggaata cataacggca acaaaaagat
     4381 agccgatggc gacgatgtgc cacattgtgt gcgtcctgtt tgaatgttaa caaaatcgtt
     4441 ataatcgcaa cattctaccg caatcctgaa aggccgtctg aaatgaggaa tgaggaaaaa
     4501 cgcgccctgc gccgcgaatt gcgcgggcgg cgttcgcaaa tggggcggga cgtgcgggcg
     4561 gcggcaacgg taaaaattaa ccgcctgctc aaacgttata tcaagcacgg tcggaaaatc
     4621 ggcgtgtatt ggccgatggg caaggaattg cgtttggacg gctttgtccg cgcggcgcaa
     4681 aaacgcggcg caaaacttta tctgccttat atcgaaccgc gttcgcggcg gatgtggttt
     4741 acgccgtatc ctgaaagcgg aatggaacgg gagcgcatac ggggcagggc gaggttgaac
     4801 gtgccgcagt ttgcagggcg caaaatccgc gtgcacggtt tgtcggtatt gctcgttccg
     4861 cttgtcggca tagaccgcga aggctaccgt ttggggcagg caggcggcta ttacgatgcg
     4921 acgcttgcag cgatgaaata ccgtttgcag gcaaaaaccg tgggcgtggg ctttgcctgc
     4981 cagttggtgg acagactgtc ggtcgaggcg cacgacctgc cgctggacgg ttttgtgtcg
     5041 gaggcgggga tattgtgttt ttagccgttc agacggcatt ttgaacggaa atgccgtctg
     5101 aaggtggaat attgggagaa aaggatgtcg gaaaagataa tactgccggt cttgtcgttg
     5161 ggtggcgtac ggctcgaacc gttggatgtg catcatgaaa cgggtttgcg cgaggcggtt
     5221 tgcgatgggg aagtttggaa gctgggagta acgtccgcgc cccatccgga tagggtggca
     5281 gactatatcg ggacagcatt ggcaacgcgt ttggcgtttg cggttgtcga tgaagaggcg
     5341 ggcagggtgg tcgggacaac ggcgtattat cattttgaac cgcagattcc gcgtttggat
     5401 attggattca catggtatgc ggcatcggca cggcggacac ggataaacac ctgttgcaaa
     5461 atcatgctgt tggattacgc atttgatgtt ttggcttgcc gttgcgtggg atggcgcacg
     5521 gatattctga accttgcctc gcaacgtgcc atcgaacggc tgggtgcgga aaaagacggc
     5581 gtgctgcgta tgcatatgct ccgtaaagac ggcagtgtgc gcgatacggt cgtgtacagt
     5641 atgctgaggg aagattggtg caaaaacagg gaaatcctga ccggcaggtt ggcagggtat
     5701 ggcgtgcagg tgtgaggcag tattttca
//
LOCUS       DAXHMX010000130         5712 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHMX010000130 DAXHMX010000000
VERSION     DAXHMX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.130"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            74..1000
                     /gene="fabD"
                     /locus_tag="ACFM4S_001742"
     CDS             74..1000
                     /gene="fabD"
                     /locus_tag="ACFM4S_001742"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6051377.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEQAIVKATFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICTESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVGRAMTAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKTAACSALTDAGQVAAFIEAH"
     gene            1139..3004
                     /gene="msbA"
                     /locus_tag="ACFM4S_001743"
     CDS             1139..3004
                     /gene="msbA"
                     /locus_tag="ACFM4S_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6051378.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDSIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLFALQTANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3104..4669
                     /gene="guaA"
                     /locus_tag="ACFM4S_001744"
     CDS             3104..4669
                     /gene="guaA"
                     /locus_tag="ACFM4S_001744"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6051379.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPSWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4731..5477
                     /gene="fabG"
                     /locus_tag="ACFM4S_001745"
     CDS             4731..5477
                     /gene="fabG"
                     /locus_tag="ACFM4S_001745"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6051380.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMVREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1397 a         1841 c         1375 g         1099 t
ORIGIN      
        1 tattttgtcc cgcctgaatt tttcgtaaaa ctcgggcaga atacctgatt atccaaccaa
       61 acaaaggaat actatgtctt ttgcattctt ctttcccgga caaggctcac aaagcctagg
      121 catgatgaac ggctttgccg agcaagccat cgttaaagcc acatttgacg aagcttccgc
      181 catattgggg caggacttgt gggcgatgat taacggcagc gatgccgaaa tcatcggtca
      241 aaccgtcaac acccagccca ttatgctcgc cgccggcgtt gccgtttacc gcgcctattt
      301 ggaagcgggc ggcaaaacgc ctgccgccgt tgccggacac agcctcggcg aatacaccgc
      361 actcgttgcc gccggcgcat tgaattttgc cgacgcggtc aaactcgtgc gcctacgcgc
      421 cgaactgatg cagtccgccg taccgcaagg cgtgggcgca atggcggcaa ttttgggttt
      481 ggaagatgaa caggtaaggc agatttgcac cgaatccgca caggatgaag tggttgaagc
      541 cgtcaacttc aactcgcccg gacaagtcgt aattgcaggc aatgccgccg ccgtcggacg
      601 cgccatgacc gccgccaaag aagccggtgc caaacgcgcc ctgccgctgc ccgtgtccgt
      661 accttcccat tgcagcctca tgaaacccgc cgccgacaaa ctcgccgaag ccctcaaaac
      721 cgtcgaaatc aagcagccgc aaatccgcgt catccacaac gccgacgttg ccgcctacga
      781 tgatgccgac aaaatcaaag acgcgctcgt ccgccagctt tacagccccg tacgctggac
      841 ggaaaccgtc aacgcccttg tttcagacgg cattgccgaa tccgccgaat gcggaccggg
      901 caaagtgttg gcgggcttgg caaaacgcat caacaaaacc gccgcgtgca gcgcgctgac
      961 cgatgccgga caggttgccg cctttatcga agcgcactga cttcgttccg caaaaagcag
     1021 cctgccctct tcaggctgct tttcacgtcc gaacggcaaa cagccgcata tttacgctat
     1081 aatccatccc gaccaaacca ccgacagcgg ctgccgttgc agtttccgcc ccaccgatat
     1141 gatagaaaaa ctgactttcg gactgtttaa aaaagaagac gcgcgcagct ttatgcgcct
     1201 gatggcatac gtccgcccct acaaaatccg catcattgcc gccctgattg ccattttcgg
     1261 cgttgccgcc accgaaagct accttgccgc cttcatcgcc cccctgatta accacggctt
     1321 ttccgcacct gccgcgccgc ccgagctgtc tgccgccgcc ggcatcattt ccaccctgca
     1381 aaactggcgc gaacagttta cctatatggt ttgggggacg gaaaacaaaa tctggaccgt
     1441 cccgctcttc ctcatcatcc tcgtcgtcat ccgtggcatc tgccgcttta ccagcaccta
     1501 tctgatgact tgggtctccg tgatgaccat cagcaaaatc cgcaaagata tgtttgccaa
     1561 aatgctgacc ctttcctccc gctaccatca ggaaacgccg tccggcaccg tactgatgaa
     1621 tatgctcaac ctgaccgaac agtcggtcag caacgccagc gacatcttca ccgtcctcac
     1681 gcgcgacacg atgatcgtta ccggcctgac catcgtcctg ctttacctca actggcagct
     1741 cagcctcatc gtcgtcctga tgttccccct gctctccctg ctctcgcgct actaccgcga
     1801 ccgtctgaaa cacgtcattt ccgactcgca aaaaagcata ggcacgatga acaacgtgat
     1861 tgccgaaacc catcagggac accgcgtcgt caagctgttc aacgggcagg cgcaggcggc
     1921 aaaccggttc gacgcggtca accgcaccat cgtccgcctc agcaaaaaaa tcacgcaggc
     1981 aacggcggca cattccccgt tcagcgaact gatcgcctcg atcgccctcg ccgtcgtcat
     2041 cttcatcgcc ctgtggcaaa gccaaaacgg ctacaccacc atcggcgaat ttatggcatt
     2101 catcgtcgcg atgctgcaaa tgtacgcccc catcaaaagc cttgccaaca tcagcatccc
     2161 tatgcagacg atgttcctcg ccgccgacgg tgtatgtgca tttctcgaca ccccgcccga
     2221 acaggacaaa ggcacgctcg cgccgcagcg tgtcgaaggg cgcatcagct tccgcaacgt
     2281 cgatgtcgaa taccgttcag acggcatcaa agccctcgac ggcttcaacc tcgacatcag
     2341 acaaggcgaa cgcgtcgccc tggtcggacg ttccggcagc ggcaaatcca ccgtcgtcaa
     2401 cctgctgccc cgctttgtcg aaccgtctgc cggcaacatc tgcatagaca gtatcgacat
     2461 cgccgacatc aaactcgact gcctgcgcgc ccaattcgcc ctcgtctccc aagacgtatt
     2521 cctgtttgac gatacccttt ttgaaaacgt ccgatatagc cgtcccgacg caggcgaagc
     2581 cgaagtcctg ttcgccctcc aaaccgccaa cctgcaaagc ctgattgaca gctccccgct
     2641 cggactgcac cagcccatcg gatcgaacgg cagcaactta tccggcggac agcggcaacg
     2701 cgtcgccatt gcccgcgcca ttttgaaaga cgcgccgata ttattattgg acgaagccac
     2761 cagcgcatta gacaacgaat ccgaacgcct cgtccaacaa gcgctcgaac gcctgatgga
     2821 aaaccgcacc ggcatcatcg tcgcccaccg cctgaccacc atcgaagggg ccgaccgcat
     2881 catcgtgatg gacgacggca aaatcatcga acaaggcaca cacgaacaac tgatgtccca
     2941 aaacggctac tacacaatgt tacgcaatat ctcaaacaaa gatgccgccg tccggacggc
     3001 ataaacaaaa tgccgtccga aatggtacaa tcgccccgac cctttcagac ggcatcatat
     3061 ccgccgaccc atccgattat cttcaatcac tgtaaaaccc attatgaccc aagacaaaat
     3121 cctcatcctc gacttcggtt ctcaagttac ccagctgatt gcccgccgcg tgcgcgaagc
     3181 ccacgtttac tgcgaactgc attccttcga tatgcctttg gacgaaatca aagccttcaa
     3241 ccccaaaggc atcatcctct ctggcggccc caattccgtt tacgaatccg actatcaagc
     3301 cgataccggt atttttgatt tgggcattcc ggttttgggc atctgttacg gcatgcagtt
     3361 tatggcgcac cacttgggcg gcgaagtgca gcccggcaac cagcgcgaat tcggttatgc
     3421 gcaagttaaa accatagaca gcgagctgac acgcggcatt caagatggtg agccaaacac
     3481 actcgacgta tggatgagcc acggcgacaa agtgtccaaa ctgcccgacg gtttcgccgt
     3541 catcggcgac acgccgtcct gcccgattgc catgatggaa aacgccgaaa aacaattcta
     3601 cggcatccaa ttccaccccg aagttaccca caccaaacaa ggccgcgccc tgttgaaccg
     3661 ctttgtcttg gatatttgcg gcgcgcaacc tagctggacg atgccgaact acatcgaaga
     3721 agccgttgcc aaaatccgcg aacaggtcgg cagcgacgaa gtgattttgg gtttgtccgg
     3781 cggcgtggac tcttccgtag ccgccgcgct gattcaccgc gccatcggcg accaactgac
     3841 ctgcgtgttc gtcgatcacg gtttgttgcg cctgaacgaa ggcaaaatgg tgatggatat
     3901 gttcgcccgc aacttgggtg tgaaagtgat tcacgtcgat gccgaagggc agtttatggc
     3961 gaaactcgcc ggcgtgaccg atccggagaa aaaacgcaaa ataatcggcg cggaatttat
     4021 cgaagtattt gatgccgaag agaaaaaact caccaacgcc aaatggctgg cgcaaggcac
     4081 gatttaccct gacgtaatcg aatccgcagg cgcgaaaacc aaaaaagccc acgccatcaa
     4141 atcgcaccac aacgtcggcg gcctgcctga aaacatgaag ctcaagctgc ttgagccgtt
     4201 gcgcgactta ttcaaagacg aagtgcgcga actcggcgtc gctttgggct tgccgcgcga
     4261 aatggtgtac cgccatccgt tccccggtcc cggtttgggc gtgcgcatct tgggcgaagt
     4321 gaaaaaagaa tacgccgact tattgcgtca agcagacgat attttcatcc aagaattacg
     4381 caatactacc gacgaaaacg gcacatcttg gtacgacctg accagccaag ccttcgccgt
     4441 cttcctgccc gtaaaatccg tcggcgtaat gggcgacgga cgcacttacg actacgtcgt
     4501 cgcactgcgc gcagtcatca ccagcgactt catgaccgca cactgggcag aactgcctta
     4561 ctcactgctc ggacgcgtgt ccaaccgcat catcaacgaa gtcaaaggca tcaaccgcgt
     4621 ggtgtacgat gtcagcggca aaccgcctgc caccatcgag tgggaataac cagcaaacag
     4681 ggctgcaccg tccggcgcag tctttcgatt atcggaaaaa aggaaaaaat atgagcacac
     4741 aagatttaag cggcaaaatc gctttggtaa ccggcgcatc gcgcggtatc ggtgcagcaa
     4801 ttgccgacac actggcggca gccggtgcca aagtcatcgg tacggcgacc agtgagagcg
     4861 gtgcggcggc gattagcgag cggttggcgc aatggggcgg cgaaggccgc gtattaaatt
     4921 ccgccgaacc cgaaaccgtc gaaaacctga ttgccgacat cgaaaaaacg ttcggcaaac
     4981 tcgatattct ggtcaacaac gccggcatca cccgcgacaa cctcctgatg cgcatgaaag
     5041 aagaagagtg ggacgacatc atgcaggtca acctcaaatc cgtgttccgc gcttctaaag
     5101 ccgttttgcg cggtatgatg aaacaacgtt ccggccgcat catcaacatc acatccgtcg
     5161 tcggcgtgat gggcaatgcc ggtcaaacca actatgccgc ggcaaaagca ggcttaatcg
     5221 gtttctccaa atccatggtg cgcgaagtcg gcagccgggg cattaccgtc aactgcgtcg
     5281 cccctggctt tatcgatacc gacatgacac gcgccctgcc ggaagaaacc cgccaaacct
     5341 ttaccgccca aaccgccttg ggcagattcg gcgacgcgca agacatcgcc gatgcggttc
     5401 tgttcctcgc ttccgaccaa gcaaaataca tcaccggcca aacgctgcac gtcaacggcg
     5461 gtatgctgat gccctgacgg gcagccgtac cgacaatgcc gtctgaagcc ctttcagacg
     5521 gcatttgcat tctcaggcaa aatgaacaca cgccacaccc cgccctgccc ctgcggatca
     5581 ggcacaagct gagacctttg caaaactcct ttccctcccg acagccgaaa cccaaacaca
     5641 ggttttcggc tgttttcgcc ccaaatacct cctaattcta cccaaatacc cccttaatcc
     5701 tccccggata cc
//
LOCUS       DAXHMX010000131         5692 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHMX010000131 DAXHMX010000000
VERSION     DAXHMX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.131"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            99..815
                     /locus_tag="ACFM4S_001746"
     CDS             99..815
                     /locus_tag="ACFM4S_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6051381.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            964..1866
                     /locus_tag="ACFM4S_001747"
     CDS             964..1866
                     /locus_tag="ACFM4S_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6051382.1"
                     /translation="MDSLMTLISVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGLSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1909..2682
                     /locus_tag="ACFM4S_001748"
     CDS             1909..2682
                     /locus_tag="ACFM4S_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6051383.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2794..4482
                     /locus_tag="ACFM4S_001749"
     CDS             2794..4482
                     /locus_tag="ACFM4S_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6051384.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4575..5531
                     /gene="gshB"
                     /locus_tag="ACFM4S_001750"
     CDS             4575..5531
                     /gene="gshB"
                     /locus_tag="ACFM4S_001750"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6051385.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1333 a         1540 c         1570 g         1249 t
ORIGIN      
        1 aaatttgtgc aatgctttaa ttcctctgac acgatttgaa acacagccgc cttaaaatat
       61 cttaaaaaat ttgaaagcgt aaaaatacgg aaaacatcat gaactttgaa aacgacgaca
      121 ttatccatgc gccgactacg tcttccctga ttctcgaaga gcggcacgat tctgagctgt
      181 tccgtgttta cgccctgatt ttggacggca ttaccgatca ggtgctgctg cccggcaaaa
      241 agctgaccga atccgaactt tgccgtcaga tggtgtgttc gcgcaacacc gtccgcggcg
      301 cgctgtcgct tttggcgcac gacaagattg tcgatttgca acccaacagg ggcgcgttcg
      361 tccacgttcc cgatttgaaa gaaatgcagg atgtgttcaa tgcgcgcatc gaaatggaga
      421 cgatgatttt gaatatcctc gcagatttgc cggatttgga aacgcgcctc aagccgcttt
      481 atgcgatgat acggcgcgaa gaagaggcct ccggcagggg cgaccgcgtt ggctggaacc
      541 gcctgtccaa tgccttccac gtcgaactgg cgcgcctggt gggcaacgat gtgctgttcg
      601 acattatgaa cacgctgtgc gcgcggtctt ccctgattgt cgccgtggcg ggcgtgcatc
      661 gcgaggaaaa acacgccatc aatacgcata cgcattcaga acaccgcgaa atcctcgacc
      721 tgctgctggc gggcaggcgc aaccgcgtgg tcaaaatcct gcgccgccat ttgggcaact
      781 gtatggagcg tttggaaaag actttggaag attgaatgcc ggagcggata agccgcctga
      841 accttcaggc ggttttttaa tggcgaacct gatgccgtct gaaatatgga tgcgggtatc
      901 tgcaattttc agacggcaat ttttaaaccg cacatatcat gcggcaataa aggaggccag
      961 gagatggaca gcctgatgac attgatttcg gttttgatac cgatgtttgc cggatttttt
     1021 atccgtgtgc ccaagcctta cctgcccgct ttggataagg tgctatcggt tttggtgtat
     1081 gccgtgctgc tgctgatcgg cgtatcgttg tcgcgcgtgg aggatttggg ttcgcggttg
     1141 gacgatatgg cgttgacggt tctgtggctg tttgtttgta cggtcgggac gaacctgctt
     1201 gctttggcag tgttgggaaa gttattcccg tggcggataa aggggaaagg gaagggcgtt
     1261 tcggtcggtg tgtcgggcag tgtggggcag ctcggatgcg tgctgctcgg atttgcatcc
     1321 ggcaaactga tgcgcgatat ttggatgccg tctgaaagcg cgggcatgta ttgtctgatg
     1381 ctgctggtgt tcctcatcgg cgtacagctc aaaagcagcg gcctatcgtt gcggcaggtt
     1441 ttggtcaacc gcaggggtat tcggttgtcg gtctggttta tgctttcatc tctttcgggc
     1501 gggctgctgt ttgccgcatc ggcagacggt gtgtcgtgga cgaaaggttt ggcgatggct
     1561 tccggcttcg gttggtattc cctctcgggt ttggtcatga ccgaggctta cggcgcggta
     1621 tggggcagca tcatgctgct gaacgatttg gcacgagagc tgtttgcact ggcatttatc
     1681 ccgctgctga tgaagcgttt tccggatgcg gcggtggggg ttggcggtgc gaccagtatg
     1741 gattttacat tgcccgtgat tcagggtgcg ggcggtttgg aagtcgtgcc ggtagcggtc
     1801 agcttcggcg tggtggtcaa tattgccgcc ccgtttctga tggtggtgtt ttccacgctg
     1861 ggctgaacgc ggtaaaatcg gcatcccgat gcaaggaggc agaaaacgat gaaaccgaaa
     1921 atccaaaggc acggagagat tttaagcctt gtccgccggc atcagtttat gtcggtggac
     1981 gagcttgccg ccgcattgga cgttaccccg cagacgatac gccgcgacat ccgcgaattg
     2041 gaggatgctg gcagcctgaa acgccaccac ggcggcgcat cctcgggcgg aaacttgccg
     2101 gaggggctgc ctgccagccg ccaaattcaa tgtcaaaacg aaaaaaacgc cattgcccgg
     2161 ctgattgcgg aacatattcc cgacgagtca tcgctgtttg tcagtatcgg cacgacgatg
     2221 gaagccgtgg catcggagct ggtgagacgg cgcggcagcc tgcggattat taccaacaat
     2281 atccacgtcg cctccatcgt ttcggcgcgg acggattaca cggtcatcat cacttccgga
     2341 gtcgtccgcc ctttggacgg cggcattacc ggcgtggcaa ccgtcgattt tatcaaccag
     2401 ttcaaagtcg attatgccgt gatgagtacg cacggcgtgg aaagcgacgg ttcgcttttg
     2461 gacgacgact acaaagaagt cagcgtgatg caggcgatga ttgccaacgc gcgcgtccgc
     2521 ttcctcggcg tggaccacag taaattccgc agcaacgcgc tggtcaggct cggcgacatt
     2581 acggcgtttg acaaagtatt taccgaccgg ctgcccgata ccgcgatgca gaagatgctg
     2641 aaagaggcgg gggtggaatg cctgattgcc gatgccgtct gaacgctatg tcaaagcgcg
     2701 caagtcgggt acaataaaca catcatcaaa ccgcttcaga cggcataagg aaccgcccca
     2761 atgccgtctg aagccatctg ttcaaagaaa actatgctca ataaagacca atttgcggac
     2821 aaccatttta tccgcacctt catcgaagaa gacctcaaaa gcggcaaaca cgaaaccatc
     2881 caaacccgtt ttccgccgga gcccaacggc tacctgcaca tcggacacgc caaatccatc
     2941 tgcctgaact tcggtttggc gtatatttac gacggcttgt gcaacctgcg ttttgacgat
     3001 accaaccccg aaaaagaaaa cgacgaatac gtcaacgcca tcaaagaaga cgttgagtgg
     3061 ctcggtttcc attgggcggg cgagccgcgt ttcgcttcca actatttcga ccagctttat
     3121 gactacgccg tcggtttggt taaagacggc aaagcgtatg tcgatgattt gacgccagaa
     3181 gaaatgcgcg aataccgcgg tacgctgacc gaagcaggca aaaacagccc ttatcgcgac
     3241 cgcagtatcg aagaaaacct cgacctgttc acgcgcatga aaaacggtga gttccccgac
     3301 ggcagcaaaa ccctgcgcct gaaaatcgac atggcatccg gcaacatcaa tatgcgcgat
     3361 ccggtcatct accgcatccg ccgcgcccat caccacaaca ccggcgacaa atggtgcatc
     3421 tatccgatgt acgactacac gcattgtatt tccgatgcca tcgaaggcat cacgcattcc
     3481 ttgtgtacgc tcgaatttga agcccatcgt ccgctgtatg actgggtgtt ggacaacatc
     3541 cccgcgccgc acgccacacg tccgcgccaa tacgagtttt cacgcttgga gcttttgtac
     3601 accattacct ccaaacggaa attgaatcag ttggttgtgg aaaaacacgt ttccggctgg
     3661 gacgatccgc gtatgccgac catttccggt atgcgccgac gcggctacac gcccgaaggg
     3721 ctgcgcctgt ttgccaaacg cgccggtatt tccaaatctg aaaacatcgt cgatatgagc
     3781 gtgttggaag gcgcgattcg cgaagagttg gaaaattccg ccccacgcct gatggcggtg
     3841 ttgaacccgc tcaaagtgac cctgaccaac tttgaaaccg gcagaaccca aagccgccgt
     3901 gccgcgttcc atccgaacca cgaggaaatg ggcgagcgcg aagtacctat ttcacaaacc
     3961 atctatattg aagccgacga ctttgccgaa aatccgccca aaggcttcaa acgcctgatt
     4021 cccggcggcg aagtacgctt gcgccacggc tatgtcatca agtgcgatga agtcgtcaaa
     4081 gacgaagcag gcaatgtggt tgaactcaaa tgcagcatcg accacgacac cttgggcaaa
     4141 aatccagaag gccgtaaagt taaaggcgtg attcattggg tttccgccga acacgccgcc
     4201 gaaatcaaag tccgtctgta cgaccgcctc tttaccgtcg agcgtcccga tgccgtgcgc
     4261 ggcgaagacg gcgaatacct gccgttcacc gatttcctca atccggaatc cgtcaaggaa
     4321 atcaccgcct acgccgaacc tgccgccaaa gatttgccgg cagaaagccg ttggcagttc
     4381 gagcgtatcg gctacttcgt aaccgaccgt aaagaccacg gcaaagacac gccggtgttt
     4441 aaccgcacgg tcacgctgaa agattcttgg cagcctaagt aaaaccccat ccttgccgtc
     4501 tgaatattgt tcggacggca tttccccttt gcccgcgaaa tgcggcacat tcggcacacc
     4561 ggaaaggaaa tatgatgaaa gtcctcttta tcgccgaccc gatggcaagt ttcaaaacct
     4621 acaaagacac cacctacgcc atgatgcgcg agatggcaaa acgcggttgg cggctgtttc
     4681 ataccttgag cggggaattg tcggttaaaa acggtctggt ggttgctgat gcgtctgctt
     4741 ttgagtttct cggcgcacaa gacgaccacg acaaaaactg gtttgccgcc gccgacaaag
     4801 ttcagacggc attgaaagca tttgatgccg tgattatgcg taccgatccg ccgttcgata
     4861 tgcaatacct ctacgccacc caattactga cgctggcgga gcagcagggc gcgaaggtct
     4921 ttaacagcgg acaggcgatg cgtgatttca atgaaaaact ggcgattttg aacttcagcc
     4981 gctttaccgc gcccacgctg gtaacgaccc gttccgccga tgtccgcgca tttttgaaag
     5041 aacacggcga catcatcatt aaaccgctcg acggcatggg cggtatgggc atcttccgcc
     5101 tgaccgaaaa agaccccaac atcggcagca tcctcgaaac cctgatgcag cttgattccc
     5161 gcaccattat ggcgcaacgc tacattcccg aaatcgtaca cggtgacaaa cgcatcctga
     5221 ttatcggcgg cgaagtcgtc ccctatgctt tggcgcgtat cccgcaaaac ggcgaaacac
     5281 gcggcaacct tgcggcaggc gggcgcggcg tggcgcagga attgggcgga cgcgaccgcg
     5341 aaattgccga aacgctcgcc cccgaactca aacggcgcgg catcctgctg gccggtttgg
     5401 acgttatcgg cagcaacctg accgaagtca acgtaaccag cccgaccgga ttccaagaaa
     5461 ttatgaaaca aaaaggtttc gacgtggcgg caatgtttgc cgatgccgtt gccgcgtggt
     5521 cggtacgtta aaccgatgcc gtttgaaagg cttttgcttc acaaccgttt ggtttgatcg
     5581 ggcaggcagg gttttccctc agccggcaga gatacgctta atgccgtacg gacacaccgc
     5641 cgccccgttt tcagacggta tatatagtgg attaacaaaa atcaggacaa gg
//
LOCUS       DAXHMX010000132         5548 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHMX010000132 DAXHMX010000000
VERSION     DAXHMX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.132"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            176..2323
                     /gene="tbpB"
                     /locus_tag="ACFM4S_001751"
     CDS             176..2323
                     /gene="tbpB"
                     /locus_tag="ACFM4S_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpB"
                     /protein_id="HFC6051386.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNWYSQVKEDEVKLDESDWEATGLPDEPKELPK
                     RQKSVIEKVETDSDNNIYSSPYLKPSNHQNGNTGNGINQPKNQAKDYENFKYVYSGWF
                     YKHAKREFNLKVEPKSAKNGDDGYIFYHGKEPSRQLPASGKITYKGVWHFATDTKKGQ
                     KFREIIQPSKSQGDRYSGFSGDDGEEYSNKNKSTLTDGQEGYGFTSNLEVDFHNKKLT
                     GKLIRNNANTDNNQATTTQYYSLEAQVTGNRFNGKATATDKPQNSETKEHPFVSDSSS
                     LSGGFFGPQGEELGFRFLSDDQKVAVVGSAKTKDKPANGNTAAASGGTDAAASNGAAG
                     TSSENGKLTTVLDAVELKLGDKEVQKLDNFSNAAQLVVDGIMIPLLPEASESGNNQAN
                     QGTNGGTAFTRKFDHTPESDKKDAQAGTQTNGAQTASNTAGDTNGKTKTYEVEVCCSN
                     LNYLKYGMLTRKNSKSAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIPSEQNIVY
                     RGSWYGYIANDKSTSWSGNASNATSGNRAEFTVNFADKKITGTLTADNRQEATFTIDG
                     NIKDNGFEGTAKTAESGFDLDQSNTTRTPKAYITDAKVQGGFYGPKAEELGGWFAYPG
                     DKQTKNATNASGNGNSASSATVVFGAKRQKPVQ"
     gene            2410..5160
                     /gene="tbpA"
                     /locus_tag="ACFM4S_001752"
     CDS             2410..5160
                     /gene="tbpA"
                     /locus_tag="ACFM4S_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6051387.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGVSQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDDSKYAYFIVEEECESKNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYYQHANRAYSSNTPPKNGNGGSNPNPNGDKDKPYWVSIGGGNVVTGQICLFG
                     NNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRT
                     LSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAG
                     IVFKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINIL
                     GKIDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGY
                     DQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYT
                     IKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEM
                     KF"
     gene            5178..5324
                     /locus_tag="ACFM4S_001753"
     CDS             5178..5324
                     /locus_tag="ACFM4S_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051388.1"
                     /translation="MPSERLQTAFFTQFPPFSIIPNNTVILKSVIPAKKAGVGCAAWA
                     QGWL"
BASE COUNT         1613 a         1406 c         1397 g         1132 t
ORIGIN      
        1 gatatacgtg ggaatgacgg gatataggtt tccgtgcgga cgcgttcgga ttcccgcctg
       61 cgcggaaatg acgggatttt ggtgtattcc ctaaaaaaat aaaaaagtat ttgcaaattt
      121 gttaaaaata aataaaataa taatccttat cattctttaa ttgaattggg tttatatgaa
      181 caatccattg gtaaatcagg ctgctatggt gctgcctgtg tttttgttga gtgcttgttt
      241 gggcggaggc ggcagtttcg atcttgattc tgtcgatacc gaagccccgc gtcccgcgcc
      301 aaaatatcaa gatgtttctt ccgaaaaacc gcaagcccaa aaagaccaag gcggatacgg
      361 ttttgcaatg aggttgaaac ggaggaattg gtattcgcag gtaaaagaag acgaggttaa
      421 actggacgag agtgattggg aggcgacagg attgccggac gaacctaagg aactccctaa
      481 acggcaaaaa tcggttatcg aaaaagtaga aacagacagc gacaacaata tttattcttc
      541 cccctatctc aaaccatcaa accatcaaaa cggcaacact ggcaacggta taaaccaacc
      601 taaaaatcag gcaaaagatt acgaaaattt taaatatgtt tattccggct ggttttacaa
      661 acacgccaaa cgagagttta acttaaaggt ggaacctaaa agtgcaaaaa acggcgacga
      721 cggttatatc ttctatcacg gtaaagaacc ttcccgacaa cttcccgctt ctggaaaaat
      781 tacctataaa ggtgtgtggc attttgcgac cgatacaaaa aagggtcaaa aatttcgtga
      841 aattatccaa ccttcaaaaa gtcaaggcga caggtatagc ggattttcgg gcgatgacgg
      901 cgaagaatat tccaacaaaa acaaatccac gctgacagat ggtcaagagg gttatggttt
      961 tacctcaaat ttagaagtgg atttccataa taaaaaattg acgggcaaac tgatacgcaa
     1021 caatgcgaat accgataaca accaagccac caccacgcaa tactacagcc ttgaggctca
     1081 agtaacaggc aaccgcttca acggcaaggc aacggcaacc gacaaacccc aaaacagcga
     1141 aaccaaggaa catccctttg tttccgattc gtcttctttg agcggcggct ttttcggccc
     1201 gcagggtgag gaattgggtt tccgcttttt gagcgacgat caaaaagttg ccgttgtcgg
     1261 cagcgcgaaa accaaagaca aacccgcaaa tggcaatact gcggcggctt caggcggcac
     1321 agatgcggca gcatcaaacg gtgcggcagg cacgtcgtct gaaaacggta agctgaccac
     1381 ggttttggat gcggtcgagc tgaaattggg cgataaggaa gtccaaaagc tcgacaactt
     1441 cagcaacgcc gcccaactgg ttgtcgacgg cattatgatt ccgctcttgc ccgaggcttc
     1501 cgaaagtggg aacaatcaag ccaatcaagg tacaaatggc ggaacagcct ttacccgcaa
     1561 atttgaccac acgccggaaa gtgataaaaa agacgcccaa gcaggtacgc agacgaatgg
     1621 ggcgcaaacc gcttcaaata cggcaggtga taccaatggc aaaacaaaaa cctatgaagt
     1681 cgaagtctgc tgttccaacc tcaattatct gaaatacgga atgttgacgc gcaaaaacag
     1741 caagtccgcg atgcaggcag gagaaagcag tagtcaagct gatgctaaaa cggaacaagt
     1801 tgaacaaagt atgttcctcc aaggcgagcg caccgatgaa aaagagattc caagcgagca
     1861 aaacatcgtt tatcgggggt cttggtacgg atatattgcc aacgacaaaa gcacaagctg
     1921 gagcggcaat gcttccaatg caacgagtgg caacagggcg gaatttactg tgaattttgc
     1981 cgataaaaaa attactggta cgttaaccgc tgacaacagg caggaggcaa cctttaccat
     2041 tgatggtaat attaaggaca acggctttga aggtacggcg aaaactgctg agtcaggttt
     2101 tgatctcgat caaagcaata ccacccgcac gcctaaggca tatatcacag atgccaaggt
     2161 gcagggcggt ttttacgggc ccaaagccga agagttgggc ggatggtttg cctatccggg
     2221 cgataaacaa acgaaaaatg caacaaatgc atccggcaat ggaaattcag caagcagtgc
     2281 aactgtcgta ttcggtgcga aacgccaaaa gcctgtgcaa taagcacggt tgccgaacaa
     2341 tcaagaataa ggcttcagac ggcaccgctc cttccgatgc cgtctgaaag cgaagagtag
     2401 ggaaacacta tgcaacagca acatttgttc cgattaaata ttttatgcct gtcgctgatg
     2461 accgcgctgc ccgtttatgc agaaaatgtg caagccggac aagcacagga aaaacagttg
     2521 gataccatac aggtaaaagc caaaaaacag aaaacccgcc gcgataacga agtaaccggt
     2581 ttgggcaaat tggtcaaaac cgccgacacc ctcagcaagg aacaggtact cgatatccgc
     2641 gacctgacgc gttacgaccc cggcatcgcc gttgtcgaac aggggcgcgg cgcaagctcg
     2701 ggttactcga tacgcggtat ggacaaaaac cgcgtttcct taacggtgga cggcgtttcg
     2761 caaatacagt cctacaccgc gcaggcggca ttgggtggga cgaggacggc gggcagcagc
     2821 ggcgcaatca atgaaatcga gtatgaaaac gtcaaggctg tcgaaatcag caaaggctca
     2881 aactcggtcg aacaaggcag cggcgcattg gcgggctcgg tcgcatttca aaccaaaacc
     2941 gccgacgatg ttatcgggga aggcaggcag tggggcattc agagtaaaac cgcctattcc
     3001 ggcaaaaacc gggggcttac ccaatccatc gcgctggcgg ggcgcatcgg cggtgcggag
     3061 gctttgctga tccacacggg tcggcgcgcg ggggaaatcc gcgcccacga agatgcagga
     3121 cgcggcgttc agagctttaa caggctggtg ccggttgaag acgacagcaa atacgcctat
     3181 ttcatcgttg aagaagaatg cgaaagcaaa aattacgaaa cgtgtaaagc gaatccgaaa
     3241 aaagatgttg tcggcaaaga cgaacgtcaa acggtttcca ccagagacta cacgggtccc
     3301 aaccgcttcc tcgccgatcc gctttcatac gaaagccggt cgtggctgtt ccgcccgggt
     3361 tttcgttttg agaataagcg gcactacatc ggcggcatac tcgaacacac gcaacaaact
     3421 ttcgacacgc gcgatatgac ggttccggca ttcctgacca aggcggtttt tgatgaaaat
     3481 agaaaatacg gaccgataag aggcaacggc aaatacgcgg gcaaccacaa atacggcgga
     3541 ctgtttacca acggcgaaaa tggtgcgctg gtgggcgcgg aatacggtac gggcgtgttt
     3601 tacgacgaga cgcacaccaa aagccgctac ggtttggaat atgtctatac caatgccgat
     3661 aaagacactt gggcggatta tgcccgcctc tcttacgacc ggcagggcat cggtttggac
     3721 aaccattttc agcagacgca ctgttctgcc gacggttcgg acaaatattg ccgtccgagt
     3781 gccgacaagc cgttttccta ttacaaatcc gatcgcgtga tttacgggga aagccacagg
     3841 ctcttgcagg cggcattcaa aaaatccttc gataccgcca aaatccgcca caacctgagc
     3901 gtgaatctcg ggtttgaccg ctttggctct aatctccgcc atcaggatta ttattatcaa
     3961 catgccaacc gcgcctattc gtcgaacacg ccccctaaaa acggcaacgg cggctcaaac
     4021 cccaacccca acggcgacaa ggacaaaccc tattgggtca gcataggcgg gggaaatgtg
     4081 gttacggggc aaatctgcct ctttggcaac aatacttata cggactgcac gccgcgcagc
     4141 atcaacggca aaagctatta cgcggcagtt cgggacaatg tccgtttggg caggtgggcg
     4201 gatgtcggcg cggggttgcg ctacgactac cgcagcacgc attcggacga cggcagcgtt
     4261 tccaccggaa cgcaccgcac cctgtcctgg aacgccggca tcgttctcaa acctgccgac
     4321 tggctggatt tgacttaccg cacttcaacc ggcttccgcc tgccctcgtt tgcggaaatg
     4381 tacggctggc gggcgggtgt tcaaagcaag gcggtcaaaa tcgatccgga aaaatcgttc
     4441 aacaaagaag ccggcatcgt gtttaaaggc gatttcggca acttggaggc aagttggttc
     4501 aacaatgcct accgcgattt gattgtccgg ggttatgaag cgcaaattaa aaacggcaaa
     4561 gaagaagcca aaggcgaccc ggcttacctc aatgcccaaa gcgcgcggat taccggcatc
     4621 aatattttgg gcaaaatcga ttggaacggc gtatgggata aattgcccga aggttggtat
     4681 tctacatttg cctataatcg tgtccatgtc cgcgacatca aaaaacgcgc agaccgcacc
     4741 gatattcaat cacatctgtt tgatgccatc caaccctcgc gctatgtcgt cggcttgggc
     4801 tatgaccaac cggaaggcaa atggggtgtg aacggtatgc tgacttattc caaagccaag
     4861 gaaatcacag agttattggg cagccgtgct ttgctcaacg gcaacagccg caatacaaaa
     4921 gccaccgcgc gccgtacccg cccttggtat attgtggatg tgtccggtta ttacacgatt
     4981 aaaaaacact ttaccctccg tgcgggcgtg tacaacctcc tcaactaccg ctatgttact
     5041 tgggaaaatg tgcggcaaac tgccggcggc gcagtcaacc aacacaaaaa tgtcggcgtt
     5101 tacaaccgat atgccgcccc cggccgaaac tacacattta gcttggaaat gaagttttaa
     5161 acgtccaaat gccgcaaatg ccgtctgaaa ggcttcagac ggcatttttt acacaattcc
     5221 cgccattttc catcattccc aacaacaccg taatcctgaa atccgtcatt cctgcgaaaa
     5281 aggcgggcgt aggatgtgca gcgtgggcgc agggctggct gtagggtggg cttcagccca
     5341 ccaatcccac cgtttccacc tattccccca actccgtcaa tgttatccat tccgcccatt
     5401 cccaccgaaa accgaaaccg ccgtattccc aaaaaccttt gatgcggtga aattggtggg
     5461 ctgaagccca ccctacagcc caccctacgg ctcgctaaac gtccaaacgc cgcaaatgcc
     5521 gtctgaaagg cttcagacgg catttttt
//
LOCUS       DAXHMX010000133         5363 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHMX010000133 DAXHMX010000000
VERSION     DAXHMX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.133"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..485)
                     /locus_tag="ACFM4S_001754"
     CDS             complement(<1..485)
                     /locus_tag="ACFM4S_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
                     /protein_id="HFC6051389.1"
                     /translation="MEDKQGMTKAVAGVMTDAPTDGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     T"
     gene            complement(556..966)
                     /locus_tag="ACFM4S_001755"
     CDS             complement(556..966)
                     /locus_tag="ACFM4S_001755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051390.1"
                     /translation="MGNTAMWMSCNPTIPILFGIQVKIFPLNQILNHIHPARYAVTFE
                     NNVDSKLRRHWVAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNK
                     ERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(1090..1443)
                     /locus_tag="ACFM4S_001756"
     CDS             complement(1090..1443)
                     /locus_tag="ACFM4S_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051391.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1679..3106
                     /locus_tag="ACFM4S_001757"
     CDS             1679..3106
                     /locus_tag="ACFM4S_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6051392.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSVQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3175..4578
                     /locus_tag="ACFM4S_001758"
     CDS             3175..4578
                     /locus_tag="ACFM4S_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6051393.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4714..5363)
                     /locus_tag="ACFM4S_001759"
                     /pseudo
     CDS             complement(4714..5363)
                     /locus_tag="ACFM4S_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1369 a         1314 c         1377 g         1303 t
ORIGIN      
        1 atggtattgt tttgtccgcc aaaatggacg cgaccgtata ggacgtcttc cgaacccatc
       61 aaccaacagc gctcataaaa acgaccgccc gagcctttgg actctttgta gataccgaaa
      121 ccgaaaactt cttcggcgag catggacgcg gcgcgaatga aatcttcgtc ttccaaaaga
      181 cttacacgaa cgccgtattt atcgaaaaag gttttttcat gaaatgaaaa gctaatttga
      241 tcaatgaaag ccgaatctga tacaccgcgc cgaagaggaa cgcctaacag gtttccttta
      301 ccgtccgtta tgtacgtttc gtaacattcg aagacttcct gaaccctgcc tgccgtttcg
      361 gtttctgtac cccccctgtt agataagggg ggaagatttg aagcggttgc cggcttcctg
      421 ccgtccgtcg gcgcgtccgt catcacgccg gcaaccgcct ttgtcatccc ttgcttatct
      481 tccatggtgc gaatcctcaa aaacgggcaa aaaaaagccc tgttacttgt agaaagtaaa
      541 ggacgttaat ttttgttaat cgtcccttct tagggacgca atatataagg gacgcaatat
      601 ataaggtttt aaaacgctaa gaacaaaatc cgataacggc tctgaagaag taagtctttc
      661 tttattgcac aagatagcaa acttccacgg cattctcgcc ccacccgtgc cgtccaagcc
      721 tttttcaaag acatagatgg ttttcttggc aatcacttcg tcagtttggc ggtcgataat
      781 ccgtatggtc gcacctgcca cccagtgcct gcgcagcttg gaatcgacat tgttttcgaa
      841 cgttaccgca taacgggcgg ggtgtatatg attaagtatt tgatttagcg gaaaaatctt
      901 tacctgaata ccgaataata tcggaatggt tgggttgcag gacatccaca tagcggtatt
      961 ccccatcgct aaaaaatcct aggaaacgag caataaaatt tacgccttcg ctggtctgta
     1021 aagccgcatt gtcccacatc gggtctctgg tttttgcatc tgccgaaacg gtacgctcag
     1081 gtactacctt caacagcata atcccttcca cattgtccgc cgtctggtaa atctttcccc
     1141 cgccgttttg cattgttcgt taaacacggc ttcggcttct ttgtattttc tgtcccactc
     1201 ttcttgtgcc tgtatttctt ctttgatcgg gccgaattgt ttgggaataa tccaaacaaa
     1261 cagcatcagg atagcggcgg cggtcaggct gcctgaaagg attttgccgg ggttccgttt
     1321 gggcttttta taggcaaagc ggacgagaaa ccaaagcaac agcagcatgg tgccccaata
     1381 gccgattgag aataggatgg ccaaaccttc taggaaatgg cgtaaatcgt ttgtggtaaa
     1441 catgggttgt tcctgtggtt aaatgtgcag gctgcttttt gccgaacctt gccgcatctc
     1501 aaaagcagcc tgcgcttcag cgttgcgtta cgcagtaaaa taatgaatat ttgtaacgac
     1561 ttgggtattt tttgtcaata ttcccgcctt tcccttaaca gctgccgcgc tttccgttaa
     1621 aattccttta catatttata ttgtttcctg tttctatatt gccaaggtta tacccgttat
     1681 gtttttctcc gccctgaaat cctttctttc tcgatacatt actgtatggc gcaatgtttg
     1741 ggcggtgcgc gaccagttga aaccgcccaa acgcacggcg gaagaacagg cgtttttgcc
     1801 cgcgcatttg gaactgaccg atacgccggt ctctgccgct ccgaaatggg cggcgcgttt
     1861 tattatggcg tttgcgcttt tggctttgtt gtggtcctgg ttcggcaaaa tcgatattgt
     1921 ggcggcggct tcgggcaaaa cggtgtcggg cgggcgcagc aaaaccatcc agccgctgga
     1981 aacggcggtg gttaaggcgg tacatgtgcg cgacgggcag catgtgaaac agggagaaac
     2041 gctggcggaa ctggaggctg tgggaacaga cagcgatgtg gtgcagtcgg agcaggcttt
     2101 gcaggctgcc caattgtcca aactgcgtta tgaagcggta ttggcggcat tggaaagccg
     2161 taccgtgccg catatcgata tggcgcaagc acggtcttta ggtctctccg atgccgatgt
     2221 gcaatcggtg caggtgttgg cgcagcacca gtatcaggca tgggcggcgc aggatgcgca
     2281 attgcagtcg gctttgcgcg gccatcaggc ggaattgcag tcggccaagg cgcaggagca
     2341 gaagctggtt tcggtggggg cgatcgagca gcagaaaaca gcagactacc gccgtttgcg
     2401 ggccgacaat tttatttcgg aacatgcgtt tttggagcag cagagcaaat cggtcagcaa
     2461 ttggaacgat ttggaaagta cgcgcggtca gatgaggcag attcaggcgg ccattgcaca
     2521 ggcggagcag aatcgggtgc tgaatacgca gaacctgaaa cgcgatacgc tggatgcgct
     2581 gcgccaggca aacgaacaga ttgaccaata ccgcggccaa acggataagg caaagcagcg
     2641 gcagcagctg atgacaatac agtcgcctgc ggacggcacg gtgcaggaat tggctaccta
     2701 tacggtgggc ggtgtggtgc aggctgccca aaaaatgatg gtgattgcgc ccgatgacga
     2761 caaaatggac gtggaagttt tggtattgaa caaagacatc ggttttgtgg aacagggaca
     2821 ggatgcggtg gtgaagattg agagctttcc ctatacgcgc tacggttatc tgacgggcaa
     2881 ggtgaaaagt gtcagccatg atgcggtaag ccacgaacag ttgggcttgg tttatacggc
     2941 ggtggtgtcg ctggacaaac ataccttgaa tattgacggc aaagcagtga atctgacggc
     3001 gggcatgaat gtcacggcgg agattaaaac gggtaaacgg cgggtgctgg attatctgtt
     3061 aagcccgctg caaaccaaat tggacgaaag ctttagggag cgataggcgg atccgtactg
     3121 ggcatttgtt atccgccggt tcggacatgc agactgcctg aaaccattgc ccggatgaca
     3181 ttgctcaatc taatgataat gcaagattac ggtatttccg tttgcctgac actgacgccc
     3241 tatttgcaac atgaactatt ttcggctatg aaatcctatt tttccaaata tatcctaccc
     3301 gtttcacttt ttaccttgcc actatccctt tccccatccg tttcggcttt tacgctgcct
     3361 gaagcatggc gggcggcgca gcaacattcg gctgattttc aagcgtccca ttaccagcgt
     3421 gatgcagtgc gcgcacggca acaacaagcc aaggccgcat tccttcccca tgtatccgcc
     3481 aatgccagct accagcgcca gccgccatcg atttcttcca cccgcgaaac acagggatgg
     3541 agcgtgcagg tgggacaaac cttatttgac gctgccaaat ttgcacaata ccgccaaagc
     3601 aggttcgata cgcaggctgc agaacagcgt ttcgatgcgg cacgcgaaga attgctgttg
     3661 aaagttgccg aaagttattt caacgtttta ctcagccgag acaccgttgc cgcccatgcg
     3721 gcggaaaaag aggcttatgc ccagcaggta aggcaggcgc aggctttatt caataaaggt
     3781 gctgccaccg cgctggatat tcacgaagcc aaagccggtt acgacaatgc cctggcccaa
     3841 gaaatcgccg tattggctga gaaacaaacc tatgaaaacc agttgaacga ctacaccgac
     3901 ctggatagca aacaaatcga ggccatagat accgccaacc tgttggcacg ctatctgccc
     3961 aagctggaac gttacagtct ggatgaatgg cagcgcattg ccttatccaa caatcatgaa
     4021 taccggatgc agcagcttgc cctgcaaagc agcggacagg cgcttcgggc agcacagaac
     4081 agccgctatc ccaccgtttc tgcccatgtc ggctatcaga ataacctcta cacttcatct
     4141 gcgcagaata atgactacca ctatcggggc aaagggatga gcgtcggcgt acagttgaat
     4201 ttgccgcttt ataccggcgg agaattgtcg ggcaaaatcc atgaagccga agcgcaatac
     4261 ggggccgccg aagcacagct gaccgcaacc gagcggcaca tcaaactcgc cgtacgccag
     4321 gcttataccg aaagcggtgc ggcgcgttac caaatcatgg cgcaagaacg ggttttggaa
     4381 agcagccgtt tgaaactgaa atcgaccgaa accggccaac aatacggcat ccgcaaccgg
     4441 ctggaagtaa tacgggcgcg gcaggaagtc gcccaagcag aacagaaact ggctcaagca
     4501 cggtataaat tcatgctggc ttatttgcgc ttggtgaaag agagcgggtt agggttggaa
     4561 acggtatttg cggaataaag caggctgaaa cggttatgaa attcccaaag cagcctgcac
     4621 cccgtttcga aagtgcaggc tgctttggga ttgatccgat actttcacat tctcattgcc
     4681 tttaaataaa gcacagttaa aaacacattt aaattaagtg cgaattaatt caaatgtata
     4741 ttcaattaaa atcaaaatag gacagtagtg catcgtcaaa tcgggcgtaa tcagacaaaa
     4801 cggttcgcag ataccgctta atattcgccc acactttctc aatcgggttg agctcaggtg
     4861 aataaggtgc aagaggcaat accttatgtc ccaatttttc cgccatttcc cgtaagacac
     4921 ccatacggtg aaatcgcgca ttatctaaaa taatcaccga tttttgagtc aatgcgggca
     4981 gtaggcattg ctgaaaccac gcttcaaaaa agactccggt catcgtattt tgataaacca
     5041 tcggagcaat cagccggttg ccgacttgtg cggacaccag agataagcgt cggtatcttt
     5101 ttccacttat ctgcgctttc actatttgcc ctttcaggct gcgggcatag ggacggaaca
     5161 ggtggcggtc aaatcctgtt tcatccaaat aaacgcgttg gtagtcagaa aattcggccg
     5221 gctgtgtcaa ataatgcgtt actttggccg ggtcttgttc tttgtaagtg gtggtctttt
     5281 tttgcgcgtt atccccatct gtttgagtgc atagcaaacg gtggctgccg tacaatcaaa
     5341 atgtttggcg atttcatgca gat
//
LOCUS       DAXHMX010000134         5345 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHMX010000134 DAXHMX010000000
VERSION     DAXHMX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.134"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            55..391
                     /locus_tag="ACFM4S_001760"
                     /pseudo
     CDS             55..391
                     /locus_tag="ACFM4S_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(412..1638)
                     /locus_tag="ACFM4S_001761"
     CDS             complement(412..1638)
                     /locus_tag="ACFM4S_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6051394.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            1793..1979
                     /locus_tag="ACFM4S_001762"
                     /pseudo
     CDS             1793..1979
                     /locus_tag="ACFM4S_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2183..2842
                     /gene="queC"
                     /locus_tag="ACFM4S_001763"
     CDS             2183..2842
                     /gene="queC"
                     /locus_tag="ACFM4S_001763"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6051395.1"
                     /translation="MSNQQALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGIKQTVLDLSLMRQITHNALMDDTAAIETAGNGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2870..3385
                     /locus_tag="ACFM4S_001764"
     CDS             2870..3385
                     /locus_tag="ACFM4S_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6051396.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAIPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSEMPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFTAATPAEAKQKALQTLLTDHVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            3392..3814
                     /gene="queD"
                     /locus_tag="ACFM4S_001765"
     CDS             3392..3814
                     /gene="queD"
                     /locus_tag="ACFM4S_001765"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6051397.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDLVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4083..4454
                     /locus_tag="ACFM4S_001766"
     CDS             4083..4454
                     /locus_tag="ACFM4S_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6051398.1"
                     /translation="MKLLSTLLVLLVTAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4451..5089
                     /locus_tag="ACFM4S_001767"
     CDS             4451..5089
                     /locus_tag="ACFM4S_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6051399.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
BASE COUNT         1367 a         1545 c         1332 g         1101 t
ORIGIN      
        1 aaaatcctgc gggattggtg tggaatttag ggctaatcta gtacagcccc ttgttttttc
       61 gatacggaac cggatagagg aaaaatcgaa cattgcgcct gccttgctat gattcacgaa
      121 gaaatctccg ccatgcctat gggctatgaa accttgatcg gcgatatggg cagcgcactg
      181 tcaggcggac aaaaacaacg catcgtattg gcgcgggcct taatattgcg aaccgaaaat
      241 cctattttta gatgcagcga ccagccattt ggatattgcc aatgaaaaag cagtcaatgc
      301 aaacttgaat ggcttgtcta tcataaaaat tatggcggca cacagaaagg aaacggtgga
      361 atcagcagat aggaaaatgt ctttaggata aaaatacagt ttcaaaaata ctcaagattg
      421 ctgctgtttt ttcgcctgag cgtcaaactc tgccagcgtc atgttcaaag tctgcaaaca
      481 cggcatcatt actgcatcga cagcttggtt cacatgatcc ctttccacag gcaacggacg
      541 gtaaacgaag agcttgaaga gttcgttcaa ctcaatcgaa tccgcccccg ttttcaacac
      601 ccaaccctgt ctgccggaat agatatagcc gtaccgcgcc agcttttcca aaagctcgcc
      661 caactcgtcg tagcccatat tgatatgccg tctgaactcc tgaacaggca aggctttgcc
      721 ttctttttgc gccgcatcca gaagcagcag gattttcaac acgtcgtcaa accgtccgcg
      781 cgagtcgaag cccctgcgga acgcttctcc ctgccagtag gagagtgaag aagtcagcac
      841 cgcgccgccc aagaccagcg tccacaacag gttcagccac aacagaaaaa acggcacggc
      901 ggcaaacgcg ccgtaaatcg agcggtagcc gtcgaaattg cccatatacc aagtgaagag
      961 ggagcgcgcg gtttccagac aaaacgctgt tgccaaagcc ccgacaaacg cctgccgcgc
     1021 gggaacgaag cggtttggca cgaagcggta cagcccccac agcaaaagcg tcatgaaggt
     1081 cagcgtcgcc gccgttcgca acgcgcccga ccactgcggc gcacctgagg caagcgcggc
     1141 atcctgtacc gagccgacca taaaggaaat gcccacgccc aaagacagcg gcccgaacgt
     1201 cagtaaagcc caatagacga gaaactgcat catccacgga cgctgggaat tgacccgcca
     1261 gatgcggttg aacgtattgt ctatcgtccg aatcagcatc agcgaggtaa cgaccagcat
     1321 cacgctgccg attgccgtca gccggttcgc ctgctcgcgg aacgcattga tatagtcgaa
     1381 caccatgtcc gcgccctgcg gcacaatggt ttggttgacg aaggagacga acgaatccga
     1441 ccagcggtcg aacacgggga aaatcgaagc gaccgccacc atcacggtca gcacggggac
     1501 gagtgccagc agcgtcgtaa acgtcatgct tgccgccgcc tgcggtacgc gttcttcatc
     1561 aaagcggcgg acgacgaacc atgcaaacgc acagatttta ttgtctgcca aaccttgcaa
     1621 acgttgtaaa aaggtcataa tttcttgccc ggtcagtaag ttgggcattg atgcccgatg
     1681 ttatagccaa gtttgccgtc aggaacaaat gcctgaactg cggctgtttc agacggcatc
     1741 ggaacaactg ttatgccgtt tgaagaccga accattttaa cggaatccgc ccatgaaccc
     1801 aaatccccct caaaatcctc gtcctctact attcccaaaa cggcagcccc cgcaatcccg
     1861 cccgccgaat cgctcgcggc atcgacagcg ttgaaggttg cgaagccgta ttgcgcaccg
     1921 tccccaaagt ctccgccgtc tgcgaagccg tcaaaaaaga tattcccgac agcggctccc
     1981 gtcctgaccg ccgaagaaaa caatatcgcc ttcgcacaaa gcaaacgctt ggcggaactc
     2041 gccgtcaagt cggcataagc cgcgtgttca gacggcatgg cgttcagatg ccgtctgaac
     2101 gcgtttgcct gtataatccg catctttact gttcaacttc gcggttcgca aacctcccgc
     2161 gttaccaaaa ctaggattcg atatgtcaaa ccaacaagcc ttggtcatct tttcgggcgg
     2221 tcaggattcg accacctgcc tgattcaggc aatccaaacc tacgggcgcg aaaacgtcca
     2281 agccatcact ttccaatacg ggcaacgcca tgccgtcgag ctggaacgcg cccgctggat
     2341 tgcgcaggat ttgggcatca aacaaaccgt actcgacttg agcctgatgc ggcagattac
     2401 gcacaatgcc ctgatggacg ataccgccgc catcgaaacc gccggcaacg gtgttcccaa
     2461 tacctttgtg gacgggcgca acgcgctttt cctgctttac gccgcgattt acgccaaagg
     2521 gcaggggata cggcacatca tcgcgggcgt gtgcgaaacc gacttctccg gctatcccga
     2581 ctgccgcgac gtgtttgtca aatcgatgaa cgttaccctt aatttggcga tggactatga
     2641 ttttcaaatc cacacgccgc tgatgtatct gaccaaggcg caaacttggg cgttggcgga
     2701 cgaaatgggc gcgctggact atatccgcga gcaaacccac acctgctata acggcatcgt
     2761 cggcggctgc cgcgaatgcc cgagctgtat cttgcgcgaa cgcgggctgg cggaatatct
     2821 ggaaagtaaa aaggccgtct gaacacgcgc aaaccataag gaatacgata tgcccaagct
     2881 ccatatgttt tacctcggcg gcaatgccgg caggtcgaat atcgaagtgc acgacatcca
     2941 atttgccgtg tgcgacgact accgcgaagc catccccgca ctcaaagccg catggttcgg
     3001 cgatgcggac aaaatccaca tcgacggttg gcaggttgtc gaatgggcgg acggttacga
     3061 catcgccgta tccgaaatgc ccaaaacgga aatgccgtct gaacacgccc cgcgcctgta
     3121 ttttgccaat gtcggcggtt atcgcgcggg gcagcttgcc gaggcgcacg cgttcgggct
     3181 gttcaccgcc gccacgcctg ccgaagccaa acaaaaagcc ctgcaaaccc tgttgaccga
     3241 ccatgttcag caacataaag acaacttaaa agacgtggac aacctgcttg cgctcgaccg
     3301 catcggcaat ttccatatcc gcctgacccc gaatccgcac ggcaaacccg ccgaaatcgg
     3361 ctttcaaggc tatttgccca tttgagaacc catgaaaatc accaaaatct tcaccttcga
     3421 ctcctctcat atgctcgacg ggcatgacgg caaatgccaa aacctgcacg ggcataccta
     3481 caaactcgaa atcaccgttt cagacgacct cgtcagaggc gggccgaaag acggtatggt
     3541 gatggacttt accgacttga aagccattgt caaacaacac attaccgacc ccttcgacca
     3601 cgccttcatc taccacggcg gcaacgggcg cgaatctcaa atcgccgcac tcttggaagg
     3661 ctggaacatg aaaaccctgc gcctgccctg ccgcaccact gccgaaaata tggcggtcga
     3721 aatgtacgac cgtctgaaaa acgcggggct gaacgtgtgc cgcgtcaaat tgtgggaaac
     3781 gccgacatcg tgtgcggagt atgaagggga gtagggaata tcttgaacgt atcgatatag
     3841 taaattccaa taagacatgc ccaaccgcgt cattcccgcg caggcgggaa tccagacctt
     3901 ggtttttcag gaatatttaa aaattgcagc aattccaaat ctttggattc ccgcctgcgc
     3961 ggaaaggacg gtttagagcg tccttatttg aatttaccgt aaaacggttt tttctcctgt
     4021 acggattccc cgttttttca gacgaccctc catatcaaat acacccatta aaaggaatac
     4081 ccatgaaact tctctccacc ctcttagtcc tccttgtcac cgccgaacat ttctacatcg
     4141 cctggcttga aatgacgcag attcccagcg aaaaagcggc ggaaatattc aagctgcctt
     4201 atgaatttat ggaacaaaag cgcgtgcaga ccctgttcgg caaccaaggg ctgtataacg
     4261 gctttctcgg catcgggctg gtgtggtcgc ggtttgccgc tccggataac gcggtgtacg
     4321 gcgcaacggt actgtttctc ggcttcgtcc tgattgccgc cgcgtggggc gcgttctctt
     4381 ccggcaacaa aggcatactc gtcaaacaag gtttgcccgc atttttggca gcggcggcgg
     4441 tgttggcggt atgaaaaaaa tcaatgttgc ccccgaaaat ccccaatacc gcatcgtcga
     4501 aatctttgaa agcctgcaag gcgaaggctg gaacacgggc atgcccgccg ttttcgtccg
     4561 cttgggcaaa tgcaacctcg cctgcggctg gtgtgatacc gattatttga cattcggcat
     4621 gatgagcctg tccgacatct taggccgtct gaaagcctat gccgcccgca acatcatcat
     4681 caccggcggc gaaccgacca tccagccgca tctcgatatg ctgctggaca tgctcaaggc
     4741 ggaaggttat ttcctctgta tcgaaaccaa cggactcaat cccgcgccgc cgcaaatcga
     4801 ctatgtcgcc accagcccca aagcctgcta cgccgccaaa tatgaaaata gctgtatcga
     4861 aacagccgac gaagtgcgga ttgttgccga tggtgatgtc cttgcgttct gcgaaaacat
     4921 ggaacgtaaa atccgcgcgc atcattacta cctttcgccc tgcgaacaag acggtgcgat
     4981 gaacatctac gacaccatcc gccaaatcgg tattttaaac agtcgccccg acgcgccggt
     5041 gcattggcag ttgagcgtgc agacgcacaa atgggcggga atagagtagt ttaagcagtg
     5101 taactcaaag ggccggcgta ccgttttgcc gatgtctaac atatgggaaa agtgtgccgc
     5161 ttccgcgtga aactgcccac atttccgctg cctgattgag gcggaacctt actgaataag
     5221 atgccgccgt tgggtacaag cccggcttcc taaattccga tggtcttttg aaccttgccg
     5281 atactctgtg ccagtgcgcg caaatggcag ggttagggaa aacgaaatgc cgtctgaaac
     5341 agcat
//
LOCUS       DAXHMX010000135         5266 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHMX010000135 DAXHMX010000000
VERSION     DAXHMX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.135"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            116..811
                     /gene="pgl"
                     /locus_tag="ACFM4S_001768"
     CDS             116..811
                     /gene="pgl"
                     /locus_tag="ACFM4S_001768"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6051400.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            792..1778
                     /locus_tag="ACFM4S_001769"
     CDS             792..1778
                     /locus_tag="ACFM4S_001769"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6051401.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1828..2676
                     /gene="hexR"
                     /locus_tag="ACFM4S_001770"
     CDS             1828..2676
                     /gene="hexR"
                     /locus_tag="ACFM4S_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6051402.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2797..4443
                     /gene="pgi"
                     /locus_tag="ACFM4S_001771"
     CDS             2797..4443
                     /gene="pgi"
                     /locus_tag="ACFM4S_001771"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6051403.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            4457..5008
                     /locus_tag="ACFM4S_001772"
     CDS             4457..5008
                     /locus_tag="ACFM4S_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6051404.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTIQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
BASE COUNT         1343 a         1469 c         1391 g         1063 t
ORIGIN      
        1 aaaaaagcag tcccgatatt tggtatcaaa agcataaaca actgttcggc cgacatattg
       61 ctcaacccgt ttccataacg gaatatgccg tctgaaataa aaaaaggaca caaatatgtt
      121 tgtttggcac gaatacgaaa atgcggcaga agcggcgcag tctttggctg acgcagtggc
      181 ggatgctttg cagggcgcac tggacgagaa gggcggtgcg gtgttggcag tttccggcgg
      241 acgttcgccg attgcatttt tcaacgccct gtcgcaaaaa gatttggatt ggaaaaacgt
      301 cggcatcacc ttggcagatg aacgcatcgt gccgaccgtc cacgccgaca gcaataccgg
      361 tttggtgcgc gaatacctgt tgaagaacaa agcggaagcg gcaatgtgga ttcctatggt
      421 ggaagacgga aaaactgaaa ccgaattaca tcccgatgct gttgtcgatt atgcactgaa
      481 acattacaaa cagcccgatg ttttggtttt gggtatggga aacgacgggc atacggcttc
      541 gattttcccg aaagctccgc agtttcagac ggcaatcgac ggttcggcag gtgtcgcgct
      601 ggtgcatacc acgcccgtta ccgcgccgca cgagcgcgtc agtatgacct tggatgcgat
      661 tgcccatacg gggcatgtgt ttttggcgat acggggcgaa gagaaaaaag ccgtgttcga
      721 ccaagccgca caaggcgaaa accgcgaata tccgatcaac ctcgttttga accatcaagg
      781 agtgaactgc catgtcttct acgccgaata aacaagccgg atatccccga ctggtcgccg
      841 atatcggcgg gacgaatgca cgctttgcgc tggaaaccgc gccgcgcgtc attgaaaaag
      901 ccgccgtgct tccgtgtaaa gactacgata cggttaccga tgcggtgcgt gcctatctga
      961 atcaaagcgg tgcaacagcc gtacggcacg cggcatttgc catcgccaac ccgattttgg
     1021 gcgactgggt gcagatgacc aaccaccatt gggcgttttc catcgaaacc acccgtcaga
     1081 ctttggggct ggacaccctc atccttttga acgactttac cgcgcaggca ttggcggtaa
     1141 cgcagacttc aagcaaagac ctgatgcagg taggcgggca aaagcctgtc gaatttgccc
     1201 ccaaagccgt tatcggcccc ggtaccggcc tgggcgtgag cggattggtg cacagccacg
     1261 caggctgggt ggctttggcg ggcgagggcg ggcataccag tttcccgccg tttgacgata
     1321 tggaagtgct gatttggcag tacgccaaaa acaaatacgg ccatgtttcc gccgaacgct
     1381 ttttgagcgg cgcgggcttg agcttggttt acgaggcttt ggctgcaaaa cagaaagcca
     1441 aacccgccaa actgatgccg tctgaaatca cggaaaaggc tttgagcggc gcgtcgcctt
     1501 tgtgccgtca gactttggac atcttctgcg ccatgctcgg cacggttgct tccaacctcg
     1561 ccctgacgct gggcgcgcgc ggcggcgtgt acctgtgcgg cggcattatt ccccgcgtgt
     1621 tggaatattt caaaacttcc ccgttccgca gccgtttcga gaacaagggc aggtttgaag
     1681 catatcttgc cgcgattccc gtgtatgtcg tcttgagcga gtttcccgga atttccggtg
     1741 cggctgcggc tctcgacaac catttgagaa acgtttaacc acagcggctc cttgcagcgg
     1801 ggctgcatta tcgaagggca tatcattatg ttaagcaaaa tcagcgaatc actggcaaac
     1861 ctttccggtg cggaacgcaa agtcgccgaa tgtgcattgg cggaacccaa atggttcgtc
     1921 catgccgccg ttgccgaaat tgccgaacgt gcgtccgtca gccagccgac cgtcatccga
     1981 ttctgccgca gcttgggtta taaagggctg cccgagttca agctcgcctt gtccgccagc
     2041 atcggtcatg agggtatgcc ctatgtccac gaagaactca acgccgacga cgatatggca
     2101 agcgtggtcg agaaagtgtt gggcaatgcc gccgcctcgc tgttgggcga acgccgcttc
     2161 ctgaaagagt cggagctgga aaacgccatt gccacgctga tgcacgcccg tcgcgtcgag
     2221 ttttacggtg tcggcaattc cggcattgtg gcacaggacg cgcagcataa atttttccgt
     2281 ttcggcatgt ccaccgtcgc ctatgtcgat acgcacacgc agctgatggc ggcatctgtt
     2341 ttgagcgatc aggatgtttt ggttgccatt tccaacacgg gttcgtctat cgaacttttg
     2401 gatgcggtca gcatcgccaa agaaaacggc gcgtctgtca tcgcactgac ccgcaacgat
     2461 tcgcctctgg cgcaacttgc cgactgcgtg ttgagcgttg ccacacagga aaatgccgaa
     2521 ctctacacgc ccatggtttc ccgcctcttg cagcttgccg tcatcgacat tctcgccatc
     2581 ggacttgccc tgcgcttggg cgatgctgcc agcctgcaat tgcagaaaag caaaaaaagc
     2641 atacacaaca agcacatcga ttacgacaaa gattgacctt cagacggcat cccacaaatg
     2701 ccgtctgaaa tgccgaacaa cggtcgtcgg cggcttgcgg cagtttccgg cagccttttc
     2761 atcccacaac aaaaaacctc attcaggagc atatagatga aacaccttca cgacttaccc
     2821 gcatggtcga aattgtggaa ccactttgac gacagcaaaa cattgcatat gcgcgaaatg
     2881 ttcgagcaag acccgcagcg tgcggaacgc tactggctgc aagtcggcgg actgacgctg
     2941 gactactcca aaaaccgcat caacgacgaa accatgtcgc ttttgttcga gcttgcccga
     3001 gaagcaggcg tgccggagcg gatgcggcag atgttccacg gcgaaaaaat caataccacc
     3061 gaaaaccgcg ccgtcctgca tgtcgccctt cgcaaccgca ccaattcgcc gattgtggtt
     3121 gacggtgaag atgtgatgcc caaagtcaac cgcgttttgc aacgtatggg cgaatttgca
     3181 cacgaagtcc gcagcggaag ctggctgggc tataccaacc aagtcattac cgacgttgtc
     3241 aacatcggca tcggcggatc ggatttgggc ccgctgatga tgtgtaccgc gctcaaacct
     3301 ttcggtcatc cgcgcctcaa tatgcacttc gtctccaacg tggacggctc gcaactgcgc
     3361 gacgtattgt ccaaagtcca ccccgaaacc acgctgttca tcatcgcctc caaaacattt
     3421 accacgcagg aaacgctgac caacgcgctg accgcgcgcg aatggttttt gaatcatgcg
     3481 ggcgacgaag aagccgttgc caaacacttc gccgccgttt ccaccaatca aaaagccgtc
     3541 gccgaattcg gcatcgacac cgccaatatg tttgaatttt gggattgggt cggcggtcgg
     3601 tacagcctgt ggtccgccat cggattgccg attatgctgt atctcggcga agaaaacttc
     3661 attgaaatgc tcaacggcgc gcacctgatg gaccaacact tcatcaacac accgctcgag
     3721 cgcaacctgc ccgtcattct cgccctcatc ggcatctggt atatcaacta ctacggcggc
     3781 ggcagccacg tcatcgcgcc ttacgaccaa catttgcacc gcctgcccaa attcatccag
     3841 cagctcgata tggaaagcaa cggcaaacag gttacgttgg acggcaaagc agtcggacac
     3901 gaaacctcgc cgattatctg gggcgaaacg ggcattaacg gccagcacgc cttcttccaa
     3961 ctgctgcacc aaggcacgca cattaccccc atcgacctga ttgcctcgct tgaaaaacgc
     4021 agcaacctgc ccggacacca cgaaatcctg cttgccaacg tcttcgccca agcagaagcc
     4081 tttatgcgcg gcaaaacccc cgacgaagtc cgcgccgaac tcaaagcgca gggtatggat
     4141 gaggcgcgca tcgaagagct ggtcccgcac aaaaccttct ccggcaaccg cccgaccaac
     4201 ctcattctca tggacaaggt caacccgcgc aatatgggca gcctgattgc catgtacgaa
     4261 cacaaaacct tcgtacaagg catcatttgg ggcatcaaca gcttcgacca gtggggcgtg
     4321 gaactcggca aacaactggc taaaaccatt ttgggcgaac tgaccggcga aaccgagccg
     4381 caaaagcacg acagttcgac cgaacgcctg atcaacctct acctgcagac caaccgcaaa
     4441 taaaacctgc ggaaaaatgc cgtctgaacg ccgaccgttt cagacggcat ttttatcgaa
     4501 caggaaaacc gtcggtaaac ttgcagagcg tgtgcaatcc cgatatgatg gtttgcataa
     4561 atttaaacat atatgttccg cagctatggc actgattaaa gagccgttgg acaaagcgaa
     4621 acaaaggaac gaagaacttg aagcggcaga agaagcggcg gcgcaggagg cattgggtcg
     4681 ggagcaggaa gtcgaccgcg tatccgaatg ggaagaacgc tacaagctgt cgcgcagcga
     4741 gttcgagcaa ttctggaaag gattgcctca aaccatacag aataagctgc aagcctcaca
     4801 gaaaacatgg aaaagcggga tggataaaat ctgtgccaac aatgcgaaag ctgaaggtga
     4861 aacgccaaac ggcataaaat tcagcgaact ggcatgcaaa acggcggaaa ccgaagcacg
     4921 cttggaagag ctgcacaacc gtaaaaaagc ccttatcgac gaaatggcca gggaagcgga
     4981 caagaaagaa ctgccaaagc ggctctgaac agcgcggttc aggcactgcc cgccgatatt
     5041 gccgaaaccg tcatgcccga gtaccgcaac tgacaaaacg gtttgaatgc caaatgcgcc
     5101 gacggcgacg aatacggcgt ggcacaatct gactgccgta ccagagaaat caatgcgaaa
     5161 accaaagaaa tccaaggtta tctgattgac tgaaacttgg atgcgggaat gtcggcggct
     5221 tttgcgtttt tgtcgttttt atagtgaatt aaatttaaac cgatac
//
LOCUS       DAXHMX010000136         5254 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHMX010000136 DAXHMX010000000
VERSION     DAXHMX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.136"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            144..2321
                     /locus_tag="ACFM4S_001773"
     CDS             144..2321
                     /locus_tag="ACFM4S_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6051405.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAKPSIETTPVNQPAVGAAMRLPRRNIAAFDKNGNEIPDSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVTIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQVTHRLGYDGFVYYSGERPSQSLPSAGTVKYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKTLTGQLIKNQYVQKKTD
                     EKKPLTIYNITAKLDGNRFTGSAKVNPDLAKNPAGNERLFFHADADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKKTETANAADTKPALPSGKHTKILDSLKISVDEATDGH
                     ARKFAISSMPDFGHPDKLLVEGREIPLVNKEQIIKLADGRKMTVSACCDFLTYVKLGR
                     IKTDRPAAKPKAQDEDSEDEIGESEENEDEISDEEGTEDEVVEDEGSEEDEVSEGDNG
                     ENEEEIAEEETEEEVDEAEEEKVEEPEEELPAEEGNGGSGSILPTPEAPKGRDIDLFL
                     KGIRTAEADIPKTGKAHYTGTWEARIGVPDKKDDQPGTTFIQKDSYANQGAKAEFDVD
                     FGAKSLSGKLTEKNDTHPAVYIENGVIDGNGFHATARTRDNGIDLSGQGSTSPQSFKA
                     NNLLVTGGFYGPQAAELGGNIIDSDRKFGAVFGAKKDNKEVEK"
     gene            2318..5152
                     /gene="lbpA"
                     /locus_tag="ACFM4S_001774"
                     /gene_synonym="tbpA"
     CDS             2318..5152
                     /gene="lbpA"
                     /locus_tag="ACFM4S_001774"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6051406.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSQDKTGQFFLVEGENTL
                     KPVAKLAGYGIYLNRQLNRWVKERIEKNQPLSAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEST
                     GFDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
BASE COUNT         1504 a         1232 c         1396 g         1122 t
ORIGIN      
        1 ggaatgacgg aatgtatctg tactgtctgc ggctcgtcgc cttgtcctga tttttgttaa
       61 ttcactataa aaacgggttg atattatctg tacatattaa tataatgata attattatta
      121 atcaaatagg aggaaaagta gggatgtgta aaccgaatta tggcggcatt gtcttgttgc
      181 ccttactttt ggcatcttgc atcggcggca atttcggcgt gcagcctgtt gtcgaatcaa
      241 cgccgaccgc gtaccccgtc actttcaagt ctaaggacgt tcccacttcg ccccctgcca
      301 aaccttctat agaaaccacg ccggtcaatc aacctgccgt cggtgcggca atgcggctgc
      361 caaggcggaa tatcgcagct tttgataaaa atggtaatga aattcccgat agtaagcagg
      421 cagaggagta tctgccgctc aaagaggagg atatcctgtt tttagacggt acgccgaaag
      481 aacaggctga caaacttaaa aaggaaatca acggacggta tcctaatgta acaatctaca
      541 cgtccgattt aaaagatgct gtgtataaat atgaacatgt ccgggccgga tatgtttata
      601 ctagatttgg aacagatgaa atcgaacaga actcaggtgg taagcaggtt acccaccgct
      661 taggttatga tggttttgta tattattctg gagaacgtcc ttcccaatct ttaccgagcg
      721 cgggaacggt gaaatattcc ggaaactggc aatatatgac cgatgtcaaa cgtcatcgag
      781 caggtaaggc ggttggcatt gacaatttgg gttatatcac attttatggt aacgatgttg
      841 gtgcaacttc ttatgcggct aaggatgtcg acgaaaggga aaaacatcct gctaaatata
      901 cggttgattt cgataagaaa actttgacag gtcaattaat taaaaatcag tatgtgcaaa
      961 agaaaaccga tgaaaagaaa ccactgacca tttacaacat tactgcaaaa ttggacggca
     1021 accgctttac cggcagtgcc aaggtcaatc ctgatttagc gaaaaaccct gccggtaatg
     1081 agcgtttgtt tttccatgcc gatgccgatc agcggcttga gggcggtttt ttcggcgata
     1141 acggagaaga gcttgccgga cggtttatca gcaacgacaa cagcgtattc ggcgtattcg
     1201 caggcaaaaa aacagagaca gcaaacgcag cagatacaaa acctgccctg ccgtctggaa
     1261 aacacaccaa aatcttggat tctctaaaaa tttccgttga cgaggcgact gatggccatg
     1321 cccgtaagtt tgccatttcc tctatgcccg attttggtca tcccgacaaa cttcttgtcg
     1381 aagggcgtga aattcctttg gtaaacaaag aacaaatcat caagcttgcc gacggcagga
     1441 aaatgaccgt cagtgcttgt tgcgattttc tgacctatgt gaaactcgga cggataaaaa
     1501 ccgaccgccc cgccgccaaa ccaaaggcac aggacgaaga ttccgaagat gaaattggtg
     1561 aaagcgaaga aaacgaagac gaaatcagcg atgaagaagg cacggaagat gaagtcgtag
     1621 aagatgaagg cagcgaagaa gacgaagttt ccgaaggtga taacggtgaa aacgaagaag
     1681 aaatcgccga agaagaaacc gaagaagaag ttgatgaagc cgaagaggag aaagttgaag
     1741 aacccgaaga agaattgccg gcagaagaag gcaacggcgg ttcaggcagc atcctgccca
     1801 ctccggaagc ccctaaaggc agggacatcg accttttcct gaaaggtatc cgcacggcgg
     1861 aagccgacat tccaaaaact ggaaaagcac actataccgg cacttgggaa gcgcgtatcg
     1921 gcgtaccaga taagaaagac gatcagcctg gcactacgtt cattcaaaag gatagctatg
     1981 cgaatcaagg ggcaaaagca gaatttgacg ttgatttcgg cgcgaagtcg ctttcaggta
     2041 agttgacaga aaaaaatgat acacaccccg ctgtttatat tgaaaacggt gtgattgatg
     2101 gcaacggttt ccacgcgaca gcacgcactc gggataacgg tattgacctt tctgggcagg
     2161 gttcgaccag cccgcagagc ttcaaagcca acaatcttct tgtaacaggc ggcttttatg
     2221 gaccgcaggc ggcggaattg ggcggtaata ttatcgacag cgaccggaaa ttcggcgcgg
     2281 tattcggtgc gaagaaagat aataaagagg tggaaaaatg aataagaaac acggttttca
     2341 gcttaccctg actgccttgg ctgttgccgc cgccttcccg tcatatgcgg caaaccctga
     2401 aacggcggca cctgatgcgg cgcagaccca atccctgaaa gaggttaccg tccgtgccgc
     2461 caaagtggga cggcgatcga aagaggcgac cggtttgggc aaaatcgtca aaacgtcgga
     2521 aacgttgaac aaagaacagg tactcggtat ccgcgacctg acgcgctacg atccgggcgt
     2581 ggcggttgtc gaacagggca acggcgcgag cggcggctac tcgatacgcg gcgtggataa
     2641 aaaccgtgtg gcggtttcgg ttgacggcgt tgcccaaata caggcgttta ccgtgcaggg
     2701 atcgttgagc ggatacggcg gacgcggcgg cagcggtgca atcaacgaaa tcgaatatga
     2761 aaacatcagc acggtggaaa tcgacaaagg tgccggttcg tccgatcacg gcagcggcgc
     2821 actcggcggc gcggtcgcct tccgcaccaa agaggcggca gacctgattt cagacggcaa
     2881 aagctggggg atacaggcaa aaactgccta cggcagtaaa aaccgccaat ttatgaagtc
     2941 gctcggcgcg ggtttcagca aagatggttg ggaagggctg ttaatccgaa ccgaacgcca
     3001 agggcgggaa acgcgcccgc acggcgatat tgcggatggg gtggcatacg gcataaaccg
     3061 tttggacgcg ttccgccaga cacacgatat tcaaagccaa gataaaacag ggcaattttt
     3121 cttagtagag ggcgagaata cactcaagcc cgtggcaaaa ttggcgggct acgggatata
     3181 tttgaaccgc cagcttaacc gctgggtaaa agaacgtatt gaaaaaaatc agcctttaag
     3241 tgctgaagaa gaagcgatgg tgcgggaggc gcaggcgcgc cacgaaaacc tgtccgccca
     3301 agcttacacg ggcggcggca ggatattgcc cgatccgatg gattaccgca gcggctcttg
     3361 gcttgccaag ctgggctacc gcttcggcgg caggcattat gtcggcggcg tgtttgagga
     3421 taccaaacag cgttacgata tccgcgatat gacggaaaaa cagtattacg gtacggacga
     3481 ggcggacaag tttagagaca agagcggggt gtacgacggc gacgatttcc gcgacggctt
     3541 gtattttgtg ccgaatatag aagagtggaa gggcgataaa aagttggtca ggggcatagg
     3601 tttgaaatat tcccgcacca aatttattga tgaacatcac cgccgccgcc gtatgggttt
     3661 gctgtaccgt tatgaaaacg aagcgtattc tgacaactgg gcggataagg cggtgttgtc
     3721 gtttgacaaa cagggcgtgg caaccgacaa caacacgctg aagctgaatt gcgccgtgta
     3781 tcctgctgtg gacaaatcct gccgcgcgtc ggcggacaaa ccgtattcct acgacagcag
     3841 cgaccgtttc cactaccgcg aacagcacaa tgttttgaat gcctcgtttg agaaatcgct
     3901 gaaaaacaaa tggacgaaac accatctgac tttgggcttc ggttacgatg cttccaacgc
     3961 gatttcccgc cctgaacagc tttcccacaa tgcggcaagg atttcggaat ccacgggatt
     4021 cgatgaaaag aatcaagata agtaccgttt gggtaagccc gaagtcgtcg aagggtcggt
     4081 ctgcggctac atcgaaaccc tgcgttcccg caaatgcgtg ccaagaaaaa tcaacggcag
     4141 caatatccac atttctttga acgaccgttt ttcaatcggc aaatattttg atttcagctt
     4201 gggtggcagg tacgaccgga aaaacttcac cacgtcggaa gaactcgtcc gcagcggacg
     4261 gtatgttgac cgttcgtgga acagcggcat cgtgttcaaa ccgaaccggc atttttcttt
     4321 gtcttaccgc gcctccagcg gcttcagaac gccctcattc caagaacttt tcgggataga
     4381 catttatcac gattatccga aaggctggca gcgtcccgcc ctgaaatcgg aaaaggcagc
     4441 caaccgcgaa atcggtttgc agtggaaggg cgatttcggc tttttggaaa tcagcagctt
     4501 ccgcaaccgt tataccgata tgattgccgt tgccgatcac aaaaccaaat tgccgaatca
     4561 ggcaggacaa ttgacagaga ttgatatacg cgattattac aatgcccaaa atatgtcgct
     4621 ccaaggcgtt aatatattgg gaaaaatcga ctggaacggt gtgtacggca aactgcccga
     4681 aggtttgtac accacattgg catacaaccg catcaaaccg aaatcggtat ccaaccggcc
     4741 ggacttgtcc ctccgcagct atgctttgga tgcggtacag ccgtcgcgtt atgttttggg
     4801 gttcggatac gaccagccag aggggaaatg gggcgcaaac attatgctga cctattccaa
     4861 agggaaaaac cctgacgagc ttgcttatct ggcaggcgat caaaaacgat attcgacaaa
     4921 aagagcgtcg tcttcttggt cgacggcaga cgtttccgcc tatctgaatc tgaaaaaacg
     4981 gctgaccttg agggcggcta tctacaatat cggcaactac cgctacgtta cttgggaatc
     5041 cttgcgccag actgcggaaa gcacggcaaa ccggcacggc ggcgacagca actatggaag
     5101 gtatgccgca ccgggcagga acttcagcct cgcgctcgaa atgaagtttt aaaggaaata
     5161 ccgtctgaaa gcttgatctg caccccaaaa gtcggactaa accgccaact gattaaggtg
     5221 caggtttttt tgattcgata taaacaagat ttcc
//
LOCUS       DAXHMX010000137         5187 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHMX010000137 DAXHMX010000000
VERSION     DAXHMX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.137"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(960..1205)
                     /locus_tag="ACFM4S_001775"
     CDS             complement(960..1205)
                     /locus_tag="ACFM4S_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051407.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            1317..1745
                     /locus_tag="ACFM4S_001776"
     CDS             1317..1745
                     /locus_tag="ACFM4S_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6051408.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1757..2233
                     /locus_tag="ACFM4S_001777"
     CDS             1757..2233
                     /locus_tag="ACFM4S_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6051409.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(2583..3071)
                     /locus_tag="ACFM4S_001778"
     CDS             complement(2583..3071)
                     /locus_tag="ACFM4S_001778"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6051410.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(3139..4194)
                     /gene="aroG"
                     /locus_tag="ACFM4S_001779"
     CDS             complement(3139..4194)
                     /gene="aroG"
                     /locus_tag="ACFM4S_001779"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6051411.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            complement(4356..4583)
                     /locus_tag="ACFM4S_001780"
     CDS             complement(4356..4583)
                     /locus_tag="ACFM4S_001780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051412.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            4861..5118
                     /locus_tag="ACFM4S_001781"
     CDS             4861..5118
                     /locus_tag="ACFM4S_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051413.1"
                     /translation="MTTQMGASRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYRH
                     KTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
BASE COUNT         1310 a         1295 c         1219 g         1363 t
ORIGIN      
        1 cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat
       61 cacactacat cgcaatgaaa aatcaggaaa aacaaaaaac ctctccgccg tcattcccgc
      121 gcaggcggaa atctagaatt ttaatgcggc aagaatttat cggaaaaaac tgaagtttaa
      181 agacctagat tcccgcctgc gcgggaatga cggtttagaa atcacctgaa acgagaaaaa
      241 gaaccgaaac cggacaagtc ggattcccgc acgggcggga aggacggaat ttcagattgt
      301 tgtttttgat tttctgtttt gtgggaataa cgggattttg gatgatgtgc atttatcggg
      361 aaaactaaaa accctccgcc gtcattcccg tgaaagcggg aatctagaat ttcaatgccg
      421 caagaattta tcggggatgt ccggggttta aaggtctgga ttcccgcctg cacgggaatg
      481 acggtttaga aatcacctga aacgagaaaa agaaccgaaa ccagataagt cggattcccg
      541 cacgggaggg aaggacggga tttcaggtta ctgtttttga ttttctgttt tgtgggaata
      601 acgggatttt ggatgatgtg cattttcggg aaaactaaaa accctccgcc gtcattcccg
      661 cgaaagcggg aatctagaat tttaatgcgg caaaaattta tcggaaaaaa ccgaagttta
      721 aagacctaga ttcccgcctg cgcgggaatg acggtttaga aatcacccga aacgagaaaa
      781 agaaccgaaa ccgaacaagc cggattaccg cctgcgcggg aatgacggct caaaagttac
      841 gaaacgaaaa ataaccgaaa ccaaacaagt cggattcccg cgcgggcggg aatgacgata
      901 agcgtaattt caatctgcca tattgtcttt tcacttgcct gaaaacagat ttcagccacc
      961 tatccctgca tccggcggct gatgtagtcg gcaatcaaat cccgcttttc ccaaaaaaac
     1021 ttctgcattt tttgttcgga atacagtttg cggaccagag tgggcaaacc gctgtggacg
     1081 atggcggcga tttctttgtt gcctttttct tcctgcattg ccgtctgaaa atccataccc
     1141 ctgcctttca gatattctgc aaggcggttg cggatgtggg aattgagttc gatggatttg
     1201 agcattttgc ctccgttttg cggtaaggat ttattcgatt tgtttatccg cctgatttta
     1261 tcacgtttcc caagcggttt gcctgtacaa taccgctttt cggcagggtg tgggcgatga
     1321 tgttttcttg gttcaagctg tttcacttgt tttttgtcat ttcgtggttt gcagggctgt
     1381 tttacctgcc gaggattttc gtcaatatgg cgatgattga tgtgccgcgc ggcaatcccg
     1441 agtatgtgcg tctgtcgggc atggcggtgc ggctgtaccg ttttatgtcg ccgttgggct
     1501 tcggcgcggt cgtgttcggc gcggcgatac cgtttgccgc cggctggtgg ggcagcggct
     1561 gggtacacgt caaactgtgt ttgggcttga tgctcttggc ttaccagttg tattgcggcg
     1621 tgctgctgcg ccgttttcag gattacagca atgctttttc acaccgctgg taccgcgtgt
     1681 tcaacgaaat ccccgtgctg ctgatggttg ccgcgctgta tctggtcgtg ttcaaaccgt
     1741 tttgacggag catcttatgc cgatagaaat cgaacgccgt tttttgattg gaaacgacaa
     1801 ctggcggcaa tacgccgacg aaccgctgct gttgaggcag ggttatctgt ctgttgaaaa
     1861 agaacgcacc gtccgtgtcc gcattgccgg aaaacaggcg tggctgacgc tgaaaggcta
     1921 tatttcggaa atcagccgca gtgagtttga atacgaaatc ccgcttgccg atgcggaaaa
     1981 gatgatggaa acgatgtgcc cgtttaaaat ggaaaaatgg cgttatccgg tcagatgggg
     2041 cggcagcctg tttgaaatcg atgtttttct tggcgataat gcgcctttgg tcgtcgcgga
     2101 aatcgagttg cccgacgaga atgccgattt tgaccgtccg gactggctgg gacgggaaat
     2161 cactgccgac ggtatgttta ccaatgccta tttgagcagg catccgttct caagctggaa
     2221 aaatgccgtc tgaaacggct tcagacggca ttctccgaca accatcgcaa atccaaagac
     2281 agcctctata tccttatttc tgctttcttt gttccattat tcccaataaa tccccataac
     2341 ctaaaatctc gtcattcccg cgcaggcggg aatccggttc tttaaacttc ggttttttcc
     2401 gataaattgc tttagctttt cgtttccgga ttcccgcttt tgcgggaatg acgatggaag
     2461 attgttgtta tttcggataa attaccctaa ctttaaaatc ccggattccc atcttcgggg
     2521 gaataacaat attttctatt tcagcccatc atttgcgggg gattttatag agagtttata
     2581 tttcaatatc tcaaataatg cacaaaagca tagcgcgtgc ctttgctgct gacacggcgt
     2641 tccgtccgct ctgcttcttt ccaatgcgtc cggtctattg cggggaaaaa tgcatctcct
     2701 tccacagaca aatccacttc ggttatccgc aaatcggtcg ccaatggcat cgcttgtccg
     2761 tatatctgcg cgccgcccat aatgacggct tcttccgcgc ctgcgcacaa tgccaatgcc
     2821 gcctccaaac ttgccgccgt ttccgcgcct gccgcgcaat aatccgcctg ccggctgatg
     2881 acgatgttcc tccgtccggg caggggtttg acgggcaggg attcccacgt tttccgcccc
     2941 ataatgacgg gtttgcccaa ggtataggct ttgaaaaatg cgaaatcttc ggggatgtgc
     3001 caaggcatag cattgcccgc cccgatgcac aggttttccg cacacgccgc aattagggtt
     3061 attttgagca tatttcctcc acaaaaatgc cgtctgaacg cttcagacgg cattttatga
     3121 cattccgtgt caaaaatctc aaccggcgcg cgccatacgt tttttgtttg cacctgccaa
     3181 caatgccaac agttcttcag tcgcgcccca accgatacac gcatcggtaa tgcttttgcc
     3241 gtacacttcc ggcttatcct gtctgccttc gaccaaatgg ctttccacca tcacgcccat
     3301 aatattgctc ccgtcctgtt ccaattgggc agcaatatct tgtgccactt ccatctgccg
     3361 ggtgtaatcc ttgcggctgt tggcgtggct gcaatctatc atcagcttat cggttacccc
     3421 tgccgcacgc agttgttccg ccgcctcgct gacgtgtccc gcatcataat tcggctcttt
     3481 tccgccgcgc aaaatgacat gacagtcggg attgccgccg gtatggacaa tggcggaatg
     3541 tccggccttg gttacagaca agaaatgatg cgaatggctc gccgcaccga ttgcgtcgat
     3601 ggcaatcttc aaattgccgt ccgtaccgtt tttaaagccg acggggcagg acagcccgct
     3661 tgccaattcg cggtgaactt ggctttcggt cgtccgcgca ccgattgccc cccaagaaat
     3721 caagtccgca taatattgcg gcgtaatcat atccaaaaac tcggtagagg caggcatacc
     3781 catattgttc agcgacaaca acaggctgcg cgcctgacgc aaaccgaaat tgatatcgaa
     3841 cgtaccgtcc aaatgcgggt cgttaatcaa acctttccaa cccaccgtcg tcctcggctt
     3901 ctcgaaataa acgcgcatca caatcaaaag ctcgttttca tactgcttgc ggagtttcaa
     3961 caaacgctcc gcatattcca acgccgcttt cggatcgtga atcgaacacg gcccgataat
     4021 gaccaacagc cgcttgtccc tgccgtgaac caaatcggaa atttcctgac gggtgcggtg
     4081 aaccaagccc gaagcctctt tggaaatcgg cagctcgtaa agatgggcaa tcggcggcaa
     4141 cagctcttta acttctttaa tcttaatatc gtctgtgggg taatggtgtg tcatcttgca
     4201 tctttcctga tgggttttta tacgcggctt aggttacatc gtttaccttg cacaaacaag
     4261 tgcaacatat tactacaaag ctattttatt tcaaaataac tgatatatct tttttcatag
     4321 attttaaatt tcatattttt aatattttag gaattttatt ccaaagaaaa agaaacagat
     4381 tgataaaaac aatacccacc attcattcgg acaaaaccgc acggaacatc cgttttccca
     4441 tttccacgac aatccggtac agaacacccc gctgccgatt ccgcctgcac atccggcaaa
     4501 atttcatacg caaggaaaat gttgaaaaca aaaagaacta attgcttgaa aaaagattta
     4561 attggaatgc gggtatttcc cacactttct ccatttattc atcctcatta taggaagaaa
     4621 aacatgaaaa aaccctgctt gcactctcac ttgcagccgt gtccgccgtt acggtagccg
     4681 atattcccga gtctaacggc aagggcaaag ccatcatcgg cgataaggaa aaacaccgtt
     4741 accaagcatg aaaaccttgc tttgaaagtc catggcgaaa tccgccaaga tttgggggac
     4801 ggatgatcct tgaccccttg ggcggatgca tatgcagatt tgcgcggcaa aaccaaagtg
     4861 atgacgaccc agatgggtgc ttcccgcgat gtcagcaaaa gcgccaaagg ttggagtgtc
     4921 ggtatcggtc tgaatgtagg caaacagttg accgacagcg tcggtctcga gtttgatcca
     4981 tactaccgtc acaaaacaat ctacaaaccc cgtgagattg tcttggacgg tgacaaaacc
     5041 aaaatgggcc gctccaaatc caacgagtac ggcttccgcg tagccgcaac gttctatagt
     5101 caattaaaat caaaatagga cagtagtgca tcgtcaaatc gggcgtaatc agacaaaacg
     5161 gttcgcagat accgcttaat attcgcc
//
LOCUS       DAXHMX010000138         5180 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHMX010000138 DAXHMX010000000
VERSION     DAXHMX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.138"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(32..448)
                     /gene="dksA"
                     /locus_tag="ACFM4S_001782"
     CDS             complement(32..448)
                     /gene="dksA"
                     /locus_tag="ACFM4S_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6051414.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(595..1404)
                     /gene="proC"
                     /locus_tag="ACFM4S_001783"
     CDS             complement(595..1404)
                     /gene="proC"
                     /locus_tag="ACFM4S_001783"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6051415.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIYIANRGAEKRE
                     RLEKELGVETSATLPELHSDDVLILAVKPQDMEAACKNICTNGALVLSVAAGLSVGTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVTLAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1435..1843)
                     /locus_tag="ACFM4S_001784"
                     /pseudo
     CDS             complement(1435..1843)
                     /locus_tag="ACFM4S_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(1843..2529)
                     /locus_tag="ACFM4S_001785"
     CDS             complement(1843..2529)
                     /locus_tag="ACFM4S_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6051416.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2649..3692
                     /gene="pilT"
                     /locus_tag="ACFM4S_001786"
     CDS             2649..3692
                     /gene="pilT"
                     /locus_tag="ACFM4S_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6051417.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3855..5081
                     /locus_tag="ACFM4S_001787"
     CDS             3855..5081
                     /locus_tag="ACFM4S_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6051418.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
BASE COUNT         1286 a         1611 c         1169 g         1114 t
ORIGIN      
        1 ggggattcat acggcatttt gtttgccgcc atcagtcggc aaactgtttt ttcatcctct
       61 ctcggcgttc ttgggactca acagataaag tggctgtcgg gcgtgccagc agccgcttca
      121 gaccgatagg ctctcccgta tcggcacaga atccataatc cccttcatca atattgcgga
      181 tggtcgcctg tattttactg agaagttttc gttcccgatc gcgggtacgg agttccaatg
      241 cgtactcttc ttcctgtgtg gcacggtcgg caggatcggg ggctgattcg tgttcttgga
      301 gatgccctgt cgtagcggaa gcattttcga tgagttcgtc ttgcattttt accagcaatt
      361 cgcggaaaaa agccaaatgg tcgtcattca tataatcgtc ttccggcccg ctccaattca
      421 aaatatcttg ttctgtcagc tttgccataa tgtatatcct ctcggttcac cgaaaaaaac
      481 aaagcaccac aaagtggctt tattcctttg atttttataa ggcttattgc ctaaggcgtt
      541 tttgaaaacg cgcatcataa cacgttttgg attggttttt ttattttctt tacattattg
      601 atattgccgt tccatttcct gcgaacggcg cacacaggca caaacgccct cgcttatggc
      661 ttcggcgaca cgatgccgcc tgaaagcttc cacggcttcg tgggttgtcc cgccttttga
      721 cgttacattt ttttgaagct tctcgaaatc ttcacccgtc tgctcggcaa gggtaaccgc
      781 tcctttaaac gttgccagac tgagcgcgcg tgcttctgcc atatcaaacc cttgtcggat
      841 ggcggcattt tgcaatgcgt ccagcagata aaacacataa gccggtccgc tgccgctgat
      901 accggtaatg ctgtgcattt gcgcttcatc ttccaaccaa acggtcaaac cgactgattt
      961 catgattcga tcggcaatcc tgcggtctgt ttccgatact tccgcttcgg catacatacc
     1021 ggatacgccc agtcctattt tcccgggcgt attcggcata acccggacaa tgcggcggct
     1081 tcccccgagg taacggctga gcgtaccgac tgacaatccg gctgcgacag aaagcaccaa
     1141 tgcgccgttg gtgcagatat ttttgcacgc ggcttccata tcctgcggtt tgacggcaag
     1201 gattaaaaca tcgtcggaat gaagctccgg cagggttgcc gaagtttcga cccccaactc
     1261 tttttccaaa cgttcgcgtt tttccgcacc ccgattggct atatagatgc ggtaaccgcc
     1321 ttgtttgacc aatccgcccg caacggcagc cgccatattg ccgccgccga gaaaataaac
     1381 attcattatt tctaccctat tcaaaattac ctgactaaat tcattcccaa ttttttacca
     1441 tcggacgcgc tcggacagac cgttgcgccg caacccctgc ttttccaagc agtcaaatcc
     1501 tccctgtttg aacgtctcaa tcaaaggttt gccgtcacgc gtacgccaat ccagcccgca
     1561 ggcttcaaac ttccctattt tcctgtaaaa gtaagattgt tgtgcggatt catattcgtt
     1621 tgccctgacg gaacgattgc cgtctgaaag cggtatctcg tagtcctcta aagacggcgg
     1681 actctcgccg ccaatatccc aaaaatcgat ggcagccggt tttctcggct tacaccagcc
     1741 ggacagggac gaacactcaa taccaacccg ccaaccggca gcctgcaacc gtaaatgcaa
     1801 gcaacgcagc cattgccgta tattttattg cccgaatttc catcagcccc ttttcccgaa
     1861 aatcgcgctg ccgatacgga cgtgtgtcgc accgcactca atggcggcag gcatatcgtc
     1921 cgacatcccc atagacagca cgtctgcctt aacgccagcc gcattgaggt cggcaagcag
     1981 tttccgcatc gtttgaaatt gcaccttcaa ctccgtttca ctgctgttgg ctttggcaac
     2041 acacatcagt ccgcgtacga cgatattcgg cagcttcgcc acttccacag caagcgcgac
     2101 tgcttcttcg ggcgcgacac cgtgcttcac cgcctcgccc gcaatattca cctcgataca
     2161 cacctgcaag ggcggcattg aggaaggacg ctgttcgctc aaccggacag cggttttcag
     2221 acggcatacg gtatgcaccc aatgcgcgcg ttcggcgaca aatttggttt tattggactg
     2281 cacatcgccg atgacgtgcc acacgatgtc ggtcaaatcc gccaactctt ccgttttgcc
     2341 gtaccactcc tgaatatagt tctcgccgaa atcacgctgt ccggcggcgt aaacttcgcg
     2401 gatgccgtct gaagggaaag tcttaccgac ggcaatcagg ctgacggaat gcggcttcct
     2461 tcccgcctgc agaaccaatt ttccgatacg gtcggacacc tcacaataac gttcttgcaa
     2521 caccgtcata gattatcccc taattaaaaa tgattaaaca gttgaaaccc ctccagtcag
     2581 ggcggtacaa tcaaggttgt tagaaccatt ccaaccaatc gaaacattat actaaacaga
     2641 gccgcattat gcagattacc gacttactcg ccttcggcgc taaaaacaaa gcatccgacc
     2701 ttcacctgag ttcgggcata tcccctatga ttcgggttca cggcgatatg cggcgcatca
     2761 accttcccga aatgagcgcg gaagaggtcg gtaatatggt aacttcggtg atgaacgacc
     2821 accagcggaa aatctaccag caaaacttgg aagtcgactt ctcgttcgaa ctgcccaacg
     2881 tcgcccgatt ccgcgtcaac gccttcaaca tcggccgcgg tcccgccgcc gtattccgca
     2941 ccattcccag caccgtctta tcgctggaag aattgaaagc cccgagcatt ttccaaaaaa
     3001 tcgcagaatc gccgcgcggc atggttttgg ttaccggccc taccggttcg ggcaaatcga
     3061 ccacgcttgc cgcaatgatc aactacatca acgaaaccca gccggcacac atcctgacca
     3121 tcgaagaccc gatcgaattc gtccaccaaa gcaaaaaatc cctgattaac cagcgcgagc
     3181 tgcaccagca caccctcagc ttcgccaacg cgctgcgttc cgcattgcgc gaagaccccg
     3241 acgttatcct tgtcggcgag atgcgcgacc cagaaaccat cggcttggcg ctgaccgccg
     3301 ccgaaaccgg acacttggtt ttcggcacgc tgcacacgac cggcgcagca aaaaccgtcg
     3361 accgtatcgt ggacgtattc ccagcgggag aaaaagaaat ggtgcgctct atgctgtccg
     3421 aatcgctgac cgccgtcatc tcccaaaacc tgctgaaaac gcacgacggc aacggccgtg
     3481 tcgcctcgca cgaaatcctg attgccaacc ccgccgtccg caacctcatc cgcgaaaaca
     3541 aaatcacgca gattaactcc gtcctacaaa ccgggcaggc gagcggtatg cagacaatgg
     3601 accaatcgct gcaatcgctg gtgcgccaag ggctgatcgc accggaagtc gcacgcagac
     3661 gcgcgcaaaa cagcgaaagt atgagtttct gacacacaac cgccttccgg ccataccgac
     3721 gggaaaacaa ggcgcaaaca cgcggggcgg gacgcagcat cccgcccggc tacctttccg
     3781 aacaagaagc gtccgccttc ctgttgaaac ctgccgccgc aaactgcaag gcttaaaccg
     3841 aaaagaagct aacgatgaat accgataacc tgcacgacat cttggacgaa atggttcaag
     3901 tgtattctca aaaaaaacaa agccgatccg aaaccccggc cgaaatcggc gcacacttcc
     3961 acccgctgct cgaccgcctg tgcgaaaccg cagaagcaca aaacgcgtcc gacatcctta
     4021 tcagcaaagg attcccgccc tcgttgaaaa tcaacagcgc attaaccccg cagccgcaaa
     4081 aggcgctgac gggcgaggaa accgccgcca tcgccgcatc gacgatgaac gccgaacaat
     4141 cggaaatatt ccggcgcgac ggcgaaatca actactccgt ccagtcgcgc agcggcacgc
     4201 gctaccgcgc caacgcctac cacagccaag gcagcgcagg tttggttttg cggcgcatca
     4261 accacgtcat cccgcaaatg caggaattgg gcctgcccga aaaactcaaa gacctcgccg
     4321 tcgcaccgcg cgggctgctg attatcgtcg ggcctaccgg ttcgggcaaa tccaccacga
     4381 tggcgactat gctcgaacac cgcaacaaaa ccctgcccag ccatatcgtt accatcgaag
     4441 acccgattga gtttatctac aaaccgcgcc gctgcatctt tacccagcgc gaaatcggcg
     4501 tcgacaccat aaactggcag acggcggtac aaaacgctat gcgccaatcc cccgacgtgg
     4561 tctgcatcgg cgaagtccgc agcagggaaa gtatggaata cgcgatgcag ctcgcccaaa
     4621 ccggccacct gtgcattttt acgctccacg ccaacaccgc gccgcagtcg ctcgaacgca
     4681 tactcaactt ctaccccaaa gaacagcaca accaaatact gatcgacatc gccctcaacc
     4741 tgaccggcat catctgccaa cgcctcgccc tcaaacaaga caaaacgggc aggacggcgg
     4801 ttgtcgactt gctcatcaac acgcccgcca tccaagactt catcctgaag ggcgacctga
     4861 tgaacatcag taaaatcatg gaaaccgcca aaaccgacgg aatgcagacg atggatcaaa
     4921 accttttcga actgtaccgt cacggcatca tcagttacga agaagccctg cgccagtccg
     4981 tttccgccaa caacctgcga ttgcacatcc aactgcacaa agaaggcaaa acgcccgaac
     5041 tcctttacga cagggtcaac ggtctcaacc tcatttcctg atccgcaaaa cccaatgccg
     5101 tctgaaaacc gcatccccgt tttcagacgg catgatttta tccgccccat tcatgtgcta
     5161 cactttatag tggattaaat
//
LOCUS       DAXHMX010000139         5173 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHMX010000139 DAXHMX010000000
VERSION     DAXHMX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.139"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(82..1338)
                     /locus_tag="ACFM4S_001788"
     CDS             complement(82..1338)
                     /locus_tag="ACFM4S_001788"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6051419.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAIGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSVLPEENELQVERFLSEHPEFEPVNCAELLQSLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(1686..2027)
                     /locus_tag="ACFM4S_001789"
     CDS             complement(1686..2027)
                     /locus_tag="ACFM4S_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051420.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2231..3088)
                     /locus_tag="ACFM4S_001790"
     CDS             complement(2231..3088)
                     /locus_tag="ACFM4S_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6051421.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3149..3964)
                     /locus_tag="ACFM4S_001791"
     CDS             complement(3149..3964)
                     /locus_tag="ACFM4S_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6051422.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4028..4198
                     /locus_tag="ACFM4S_001792"
     CDS             4028..4198
                     /locus_tag="ACFM4S_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051423.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            4208..4993
                     /locus_tag="ACFM4S_001793"
     CDS             4208..4993
                     /locus_tag="ACFM4S_001793"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6051424.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            complement(4974..>5173)
                     /locus_tag="ACFM4S_001794"
     CDS             complement(4974..>5173)
                     /locus_tag="ACFM4S_001794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051425.1"
                     /translation="IVQGSPFNQIWVSIFGSFGHLAVSTFLPPLPVKMPSEISARYFR
                     RHTRPFSRIPGSGLKFKRVRK"
BASE COUNT         1159 a         1206 c         1415 g         1393 t
ORIGIN      
        1 tttttgttaa tccactataa ttgatgaaat gccgtctgaa cgctttgaaa agggttcgga
       61 cggcattttg ttcaaaccgg tttatttgcg ttgcaatacg gcggcgaaga agccgtcggt
      121 ttggtgtcgg gcggaatcga ggcgcaggta ttttccggta tccaaatcaa ccttcaggct
      181 ttgcagcagt tcggcgcagt tgacgggttc aaattcggga tgttcggaca ggaaacgttc
      241 gacttgcagc tcgttttctt ccggcaacac gctgcaggtg gcgtacacca agcggccttg
      301 cggtttcacc agtttggatg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc
      361 cacggtctcg gcggattggc ggtatttgag gtcgggattg cggcgcagcg tgcctaagcc
      421 ggagcagggc gcatccacta aaacgcggtc ggctttgcct gccagtcggg caatacgggt
      481 atcgtgttca ctgctgatgc gttcagggtg gatgttggtc agtccggcac gggtcatgcg
      541 cggtttgagg ttggcaaggc gtttttcggc gatgtcgaag gcgtagattc tgcctttgtt
      601 cgccatttgc gcgccgatgg cgagggtttt gccgcctgcg ccggcacaga aatcgacaat
      661 gatttcgccg cgttttgcgc ccaccaataa agcaagcagc tggctgcctt cgtcttggac
      721 ttccagtgtg ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg
      781 gatgccccaa ggcgaataag gcgttgcctc cgcatcggca ctttcggctt gcaacaatgg
      841 cagcacttta tcgcgtttgc ctttcaaggt attgacgcgg atgtcgagcg gcgcggcttg
      901 gttgatgctg cgtccgaagg cgaggatttc ttcttcgttc caatgctgtt gcagttgttc
      961 gaccagccat tgcggcaatt ctgcggcggt attcagaccg tctgaaaact cggttttgcg
     1021 ggctttcaaa ttgccgagga actctgtttc ttcttcatca agcaggtctt tgatttggct
     1081 gatgttggtg cttctgccga gaaccagtgc ggcgagtgcg gctttgcgcg gctgcgcgtg
     1141 aggacgacgc agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcagtttc
     1201 ggcgatttcg tggcggtctt ggcggccgag ttttttgtgt tcgcggaaat aggcggagag
     1261 gacggcatcg gcaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc
     1321 gagttgtgcg gcgttcataa tgtaatgggt tctcttactg tgggttattc ataatcaatc
     1381 taacgtcctt aaaataacaa tagcatcttt atcattactt agtattctat ccgcaataag
     1441 aaagatgtgt taaaagtgcg caatcgggat ttgtctaaat ttctccgtta actacaaagg
     1501 cagcctgaaa aaagttctat tttcaggtcg cttgaacagc atccgatata atcacatcag
     1561 cgatttcatt ttgtaatcac tccctaaaac atgaggaaaa aaacaaaatt tattttcttc
     1621 gttcagtata tctatcctaa atatgccatc actccattga gtttttaatt tgtatttcgc
     1681 tcttgttaaa tgtttttctg tttgccgatt tcttttgcta atgtaaaaaa tactctacca
     1741 ctctcattgg ttaaagtttt caagtattca tcaacatcag taacattaat aacaactgtt
     1801 tcatttgtat gtttattaaa ttctaaatca ataataatat tcccattaac caatatttca
     1861 tatatatctt cttcttcagg gagcatagac aaacaataag cacttgaagc tgaaaactct
     1921 tttttcagat actgaagaag aaaagtattt ttaattaaaa tattattaaa tttatctaat
     1981 gattgcttga cttctttagt tgtccaattt ttaacattat tcttcattgt attctacctg
     2041 gggtcgtagt tattaaatta ccatacttat ttgtttggat cgtcataata ttagttggca
     2101 tgttgttgtt atatttatca tatccaatat ccttattaac tcggatgact ctttcataaa
     2161 ttgtatggga aatgagattt ccatcactaa gcctctcaat agatatacgc gttaatgttg
     2221 ggaaagggtt tcagacggcc ttcataatgc gttcgatttc gtgccaaaag ccgtccggcc
     2281 gacattccaa aacggcaatc aagccttggt cgatacggcg gatttcggtt gcgttcaatg
     2341 tttcggtttc ggcgacacgc gcaggcgcga ggtaggccat acggtcgagc agatggtagc
     2401 ggacttcttg gcgtttaaac ccgtattccg cccaatcttg aatatagatg ccgcgccaac
     2461 cgtatggatt aagcggtggc tcaaaagcat ggaaaccgtg tggaaaaaat ccgatgccgc
     2521 gcacgatgga aacgggtttt acgtcaagcg gcagaccgtg ttgccgcaag gttcggatgc
     2581 cgtctgaagt gtgcggcaga ccgagttggg cggtcagttt ggcggctttt cccaacagcg
     2641 tgtctttggg gtcgagtccg cgcatgtctt cgagcctgcc cgtgccgccg ccgtaatatt
     2701 tgcaggtcag ctcgatatgg tagggcttgc cgttaagcct taccacaaaa tccgccgcgc
     2761 cttgcgtatt gccgtccgaa ccggaaaccg tgaggttgtg cgcgagcagt tcggcgtgtg
     2821 gcgcgttggc aaaccaaaat gccagcagtt cttcggcgta aatgccgagg cggtgtccga
     2881 acggggcgcg tcgggtgagg tgttccgtca gcggcgcggg atcggcatcc aatgccaaaa
     2941 gatagcggaa accgcgttcc cccaatagtt cgcgcacgcc caattcgcaa ccgctttgcc
     3001 acaaaggcgg cgcagtcagc agcgaggcga ggtcgcggac gggttggctg gtgagcttcc
     3061 accataatgc gtctagggcg taattcatgc ttatccgaat atcagtcatg ccgtctgaaa
     3121 agctggaaat gttttcagac ggccttaatc agtctttttt atggatacga tggctgacgg
     3181 tattgaaaaa accgcgcagg atatcgatgt cttcggtttg cgtgttggcg cgtccgaaca
     3241 ggctctgcat acggcgcatc agacgctcgc cgttgcggcg gttgaaaaag ccgatgtcgt
     3301 tcatcacgct ttccatgtgg gcgaccatgc ctttgatttg ctcgtgggtc gcagcgtggt
     3361 cttcttgttg aagatgggtc atgggcgaac cggtttggct gaagatttcg tagcacacga
     3421 cctgcacggc ttgggcgagg ttgagcgaga aatagtcggg attgccgttg atggtcatca
     3481 gtcggttgca ggcttggact tcttcgatgc tcaagccgaa agtctcgttg ccgaaaacca
     3541 gtgccacttt ctcgcctcgg tttgcggtct gcagtaattc ggataccaaa tcgcgcgggg
     3601 tttgcagcgg cgcagtaatt tcgcggcggc ggctggtcag ggcgcaggcg atggtggtgt
     3661 cggcaagggc ttcgtccaaa gaagcggcaa tggtggcatt ttccaaaacg tctgccgcgc
     3721 cggaagcgag gatgaagctt tcttccggta atttaaacga ttgaggatgc tccgggtcaa
     3781 acacgggcgg gttttccgtc atcggcgttg ccatcagatt tggggcgacg atggtcagtt
     3841 tgtgcagacc cattgttttc atcgcgcgcg cggcagagcc gatgttggcg ggatggctgg
     3901 tgcgcgtgag gatgatgcgg atgttgtcca gataagcggg cagggcgggt ttgagagtag
     3961 tcatgttcgg ttttcggtcg gaatggaata gggtataatg cgccgtcttt ttcgttcgga
     4021 cggtcaaatg ccgtctgaaa tgttctttaa caacgtcgga aatttaacgt tgcgcctgaa
     4081 gcagacacgc gtcgggcggt attttacccg atttggcagg atttacccca accaaataaa
     4141 ctccgatttt gccgctccga aggactgcgc gtccgaaccg gcggtatgcc acccttaagg
     4201 aaatctgatg aatccgtttt tgaatacagc ctttaaagcc gcccgccgtg ccggtcagat
     4261 gatgatacgc gccgcgggca atctcgatgc cgtcaaaacc gacagcaaag cattcaacga
     4321 ttttgtttcc gatgttgacc gcaattccga aatcatcctg gttgaggctt tgaaagaagc
     4381 ctatccgcac cacaaaatca cttgcgaaga aagcggctcc cacggtaaag ccgcagccga
     4441 atacgaatgg attatcgatc cgctcgacgg cacgaccaac ttccttcacg gccatcccca
     4501 atacgccatc tctatggcac tcctgcacaa aggcgtgttg caagaagctt tggtgtacgc
     4561 ccccgaacgc aacgacgttt acatggcttc gcgcggcaaa ggcgcgttgc tcaatgaccg
     4621 ccgcatccgc gtttccaacc gcatcgaatt gaaccgctgc ctgatcggta ccggctttcc
     4681 tgttgtcgat caaagcatga tggacaagta tctggcgatt ttgaaagatt tcttggcaaa
     4741 aaccgccggc ggccgtcgtg aaggcgcggc ttctttggat ttgtgtgctg tggcgactgg
     4801 tcgttttgac ggctttttcg agttcaacct caaaccttgg gatattgccg caggcgcatt
     4861 gattgttcag gaagcgggcg gtatcgtaac cgatatgacg ggtgaagacg gctggctgga
     4921 aagcggcgat attgttgccg ccaatcccaa agtgctggcg caaatgttaa aaatcatttc
     4981 cgcacacgtt taaatttcag cccgctcccg ggaatgcggg aaaacgggcg ggtatgccgt
     5041 ctgaagtatc gcgccgatat ttcagacggc atttttactg gcagcggggg caggaaggtg
     5101 gaaacggcta aatgcccgaa actgccgaag atggaaaccc atatctgatt aaacggagaa
     5161 ccctgtacga ttt
//
LOCUS       DAXHMX010000140         5113 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHMX010000140 DAXHMX010000000
VERSION     DAXHMX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.140"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..1367
                     /gene="mafB"
                     /locus_tag="ACFM4S_001795"
     CDS             <1..1367
                     /gene="mafB"
                     /locus_tag="ACFM4S_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6051426.1"
                     /translation="YRLNWKGHEHHPADGYDGPQGGNYPKPTGARDEYTYHVNGTARS
                     IKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANKKIFEHNAKLDRWGNSMEFVNGIA
                     AGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSKSYTCSF
                     HGSTLVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTTRYGNPYQETVYIEVSDG
                     IGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITLKQQPLKAYNL
                     TVADWHTYFVKGDKAETEGVWVHNSCPPYKRPNNATTKAQRESVQGKPCVECGKLAEK
                     MIADHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGGRLSQYSKEQKRKLSN
                     GNKK"
     gene            1351..1704
                     /locus_tag="ACFM4S_001796"
     CDS             1351..1704
                     /locus_tag="ACFM4S_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051427.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1694..2017
                     /locus_tag="ACFM4S_001797"
     CDS             1694..2017
                     /locus_tag="ACFM4S_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC6051428.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKSMIIEFFIEGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFDIAQKNDCNYEGF
                     GSYGN"
     gene            2252..2521
                     /locus_tag="ACFM4S_001798"
     CDS             2252..2521
                     /locus_tag="ACFM4S_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051429.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPIGILPKPVALSIVQYEDD
                     QGFYLFYLDETGQEQTDTYHDTLDSAFEQVEFEFGISKEEWMQSP"
     gene            2503..2655
                     /locus_tag="ACFM4S_001799"
     CDS             2503..2655
                     /locus_tag="ACFM4S_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051430.1"
                     /translation="MDAKPLGGNIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            2642..3187
                     /locus_tag="ACFM4S_001800"
     CDS             2642..3187
                     /locus_tag="ACFM4S_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6051431.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFVW"
     gene            3321..3950
                     /locus_tag="ACFM4S_001801"
     CDS             3321..3950
                     /locus_tag="ACFM4S_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6051432.1"
                     /translation="MVKTADGYKAIAHIRVGESVLSKDEASGKTGYKPVTAQYGNPYQ
                     ETVYIEISDGIGNSQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESKIAELTNKIIPGMSKNERLKIERTIRNITKNANRKAKGEEHGRRGR"
     gene            3931..4386
                     /locus_tag="ACFM4S_001802"
     CDS             3931..4386
                     /locus_tag="ACFM4S_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051433.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITEYKNILETFMYEPPDEF
                     RTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            4393..4449
                     /locus_tag="ACFM4S_001803"
                     /pseudo
     CDS             4393..4449
                     /locus_tag="ACFM4S_001803"
                     /inference="COORDINATES: protein motif:HMM:NF026864.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin C-terminal domain-containing protein"
     gene            4464..4841
                     /locus_tag="ACFM4S_001804"
     CDS             4464..4841
                     /locus_tag="ACFM4S_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6051434.1"
                     /translation="MCELKNFRRNITCFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVRFDYYNKK"
BASE COUNT         1699 a         1033 c         1067 g         1314 t
ORIGIN      
        1 tataccgcct caactggaaa gggcacgaac atcatcccgc cgacggctat gacggcccgc
       61 agggcggcaa ttaccccaaa cctacgggcg cacgagacga atacacctat cacgtcaacg
      121 gcacagcccg cagcattaaa ctcaatccga ccgacacccg cagcatccgg caacgcatat
      181 ccgacaacta caacaacctc ggcagcaatt tctccgaccg cgccgatgaa gccaacaaaa
      241 aaatattcga gcacaatgcc aagctcgacc gctggggcaa cagcatggag tttgtcaacg
      301 gcatcgccgc cggagcactc aaccccttta tcagcgcggg cgaagcctta ggcataggcg
      361 acatactgta cggaacgcgc tatgccatag acaaagccgc aatgcgcaac atcgccccct
      421 tacccgccga gggcaaattc gccgtcatcg gcggcttggg cagcgtggcg ggctttgaaa
      481 agaatacgcg cgaagccgtt gaccggtgga tacaggaaaa ccccaatgcc gccgaaaccg
      541 tcgaagccct ggtcaacgtc ctgccgtttg ccaaagtcaa aaacctgaca aaggcggcaa
      601 aaccggggaa ggctgcggtt agtggggatt tttctaaatc ctacacctgc tccttccacg
      661 gcagcacctt ggtcaaaacg gcagacggct acaaagccat tgcccatatt caagccggag
      721 accgcgtcct ttccaaggac gaggcaagcg gagaaacggg atacaaaccc gttaccaccc
      781 gatacggtaa tccgtaccag gaaaccgttt acattgaagt ttcagacggc atcggcaaca
      841 gccaaaccct gatttccaac cgcatccatc cgttttacag tcaaggaaaa tggatacagg
      901 caggtcgtct gaaaaaaggc gacaccctgc tttccgaaag cggcgcaaaa cagacggttc
      961 aaaacattac cctcaaacag cagccgctca aagcctacaa cctgaccgtt gccgactggc
     1021 atacttactt cgtcaaggga gataaggcgg aaacggaagg ggtgtgggtt cataattctt
     1081 gtccgccata taagagacca aataacgcaa cgacaaaagc acaacgagaa agtgtacaag
     1141 gaaaaccctg cgttgaatgc ggtaaattag ccgaaaaaat gattgccgac cataaaaaac
     1201 cattagttgt cgagtattat gaaacaggga caattgacaa atctaagatg agagcaattg
     1261 aatcagtaca accgcaatgt ccgacttgtt ctgcaaaaca aggagggaga ttaagccaat
     1321 attcaaaaga acagaaaagg aaactgagta atgggaataa aaaatgaaca aataataatt
     1381 tgtaaaaaat ataatacaga aatatatcct gtttccgatg tttcaaaaat cggtgttgct
     1441 gaaaatgtca aacaggcaaa cctgtatccg attaacgggt taaggcaccg tcccaaaggt
     1501 gatactaacg gttggtatat ctgggccggt gaaaacttct cctatgataa aaattttttc
     1561 ctgcccttgc atactttcca tttgcaaata tggcgacccg aaatcatccc ctttttgaca
     1621 cttccgcccg gttaccgttt tttaattggg gaaaacggtt atgaagacgt atggtttgat
     1681 gagttgctat taaatgataa ttgacgaaaa atctacttca gatgtactga aacaatatca
     1741 agaactaggg tttgatttga caaaatcgat gataatagag ttttttatag agggttctca
     1801 aaaaaatctg caatcaatag aaagccaact catttcttat cggcaagatt ttcaaatcag
     1861 tattgaacaa gatgaattcg gagaaatgtg gacttgctat tgttccgtca atatcgtccc
     1921 ttcccttgaa aacattttag ctattgaaac tacgttattt gatattgcac aaaaaaatga
     1981 ttgtaattat gaaggttttg gttcgtatgg gaactaacaa gaagtgtagg ttgggttgaa
     2041 aaacacaaca atcaaaaggt cgtctgaaac catgtttaat tttcagatgg cttttccttc
     2101 atttaaaaca ggatattgag atctaagttc tttaaaaatc ctacacctgc tccttccacg
     2161 gcagcacctt ggtcaaaacg gcagacggct acaaagccaa tggacgtgta atagaaaata
     2221 gtgcagtaac aattattcgg attaaaaaat tatgagtgta aaagaattat tcaaagttat
     2281 cttagataaa aataaagatt ttcctattag aacgattcat cgcacgccaa tagggatact
     2341 tcccaaacct gttgctttaa gtattgttca atatgaagat gaccaaggat tttatttgtt
     2401 ttatttggat gagaccggtc aggaacaaac ggatacttat catgacacat tagattcggc
     2461 atttgaacag gtggagtttg aatttggaat tagcaaagaa gaatggatgc aaagccctta
     2521 ggtggcaata tcaagatgcc gctttatttt tcagacggca tttccgtcaa taaaaaagtc
     2581 ggtattggag cccaaaacag cctacgtctt gccgggatag ggatgaaatc tttaagaaaa
     2641 aatgattata gttgaacatt tagaacatta tttgggtgaa atcgaaagcg gtataaaatg
     2701 cttggatcga cgttatcatc tcagcgtttc cgtcttccca tcacaaccat ataaaggggt
     2761 aactactttt tctactttag gcttgaatcg atatgacttg aactataaga gtaggtttga
     2821 attaattttt acctgttctg aagaatggaa taaagaaaat attgcagctt ttctatcagg
     2881 agtagccgaa tatttgattg acaatagaca acctatttta agaggtgaaa ttattcaatt
     2941 acctagggta attatcgaag gtagcaaaat ggacgcactg tatgtttccg ccccatttta
     3001 tttcgacgat gattttcagg tctgttatgg cgaacactac aatatcgttt tccctttgct
     3061 tgtcccgttg tataaacagg aagcagaatt ggtggaaaag aaaggttgga atgcttttga
     3121 gcagttcttg ctggataatg aagttggcaa cctttcggat atgaatcgga aaccgtttgt
     3181 ttggtaaggg ctttcttaaa tttgattaaa ggtcgtctga aaccgtgttt aactttcaga
     3241 caacctttcc ttcatttgaa acaggatatt gaaaactaag tttttcaaca ttctacacct
     3301 gctccttcca cggcagcacc ttggtcaaaa cggcagacgg ctacaaagct attgcccata
     3361 tccgagtcgg cgagagcgtt ctttccaagg acgaggcaag cgggaaaaca ggatacaaac
     3421 ccgttaccgc ccaatacggt aatccgtatc aagaaaccgt ttacattgaa atttcagacg
     3481 gcatcggtaa cagccaaacc cttgtttcca ataaaatcca cccgttttac agtcaaggaa
     3541 aatggataca ggcaggtcgt ctgaaaaaag gcgacaccct gctttccgaa agcggtgcaa
     3601 aacagacagt tcaaaacatt accctcaaac agcagccgct caaagcctac aatctgaccg
     3661 ttgccgactg gcatacttac tttgtcaaag gcgataaggc ggaaacggaa ggagtttggg
     3721 ttcataattc ttgtccgcct aaaagaacag gaagctccaa gaatgaaaaa catggagatg
     3781 gcggtcgaag tcaaatatca gcggaatcaa aaattgctga attaacaaat aaaattattc
     3841 ccggaatgtc caaaaatgaa cgcttaaaga ttgagcgaac aatcagaaat attacaaaaa
     3901 atgccaatcg aaaagcaaaa ggagaagagc atggtcgacg cggtcgttaa aactcccgaa
     3961 tttttacctt ttacttctgt aggaatattc cgttttggtg cggatataac agagtacaaa
     4021 aacatactgg aaacttttat gtatgaacct cctgatgagt ttagaacaga atattatgaa
     4081 tctcccgact caaatttact catttccgtc aaaaaaaaca aaattatttc gatattttgt
     4141 tatcaggagc tatattttat gggcgttaat ataataggtc tgaattttga agattttaaa
     4201 cagcttttcc atcaccctaa atcatacggt gttgataaat attatttatc aaatgaatct
     4261 tatccgacat atgtctatga gtttgatgaa ataggagtac aagcatggga agcaaaaggg
     4321 aaaattgtta ctatcattgc cgggggcaaa gacaattatt caacagaacc ttactacgat
     4381 gaataacatt acagaaaaga aacaagaaac ggtacatttg ataaaaacct aaatcgtatt
     4441 ggagattaat tattatgtgt attatgtgtg agttaaaaaa cttcagaaga aatattactt
     4501 gttttgaggg atatgacgaa aattcattta ttggcaaatg gtatgatgac ggggtgtggg
     4561 atgatgaaga atattggaaa ttggagaatg atttaatcga ggttagaaaa aaatatcctt
     4621 atccgatgga tataccaaga tatgttgtca ttggaatcgg taccattatt gatttcttaa
     4681 tggttccaaa ttggaaactt tttgaaatta aagcttcccc ttggctacct gatagcgtag
     4741 gaattaatga acgttatgaa aggcttaaaa caatgctccg ttatattttt accgagaaag
     4801 acatagtcaa cgtgcgattt gattattaca acaaaaaata gcaaaccccg gcaatcaaaa
     4861 tgccgtctga agcgatattt gcttttcaga cggcattttg atgaatgaaa caggatattg
     4921 agaactaagt tcttcaaaaa tcctgcctcc gtcccttata cgtcggtgct ttagtcaaaa
     4981 cggcagacgg ctgaaaagca aacactgtca tcaaccattt gaagaccgga tggaatagga
     5041 aagaaaatcg gtaaaatctc cacgattaga cggtaaaata cctcggtgtc gctgaaaccc
     5101 tgatcgtcgt gtt
//
LOCUS       DAXHMX010000141         4996 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHMX010000141 DAXHMX010000000
VERSION     DAXHMX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.141"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(29..670)
                     /locus_tag="ACFM4S_001805"
     CDS             complement(29..670)
                     /locus_tag="ACFM4S_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6051435.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLNDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(660..1211)
                     /locus_tag="ACFM4S_001806"
     CDS             complement(660..1211)
                     /locus_tag="ACFM4S_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6051436.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDINTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(1215..1733)
                     /locus_tag="ACFM4S_001807"
     CDS             complement(1215..1733)
                     /locus_tag="ACFM4S_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6051437.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDNTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(1733..3394)
                     /gene="pqiB"
                     /locus_tag="ACFM4S_001808"
     CDS             complement(1733..3394)
                     /gene="pqiB"
                     /locus_tag="ACFM4S_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6051438.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFGKLPLNKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(3387..4721)
                     /locus_tag="ACFM4S_001809"
     CDS             complement(3387..4721)
                     /locus_tag="ACFM4S_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC6051439.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGNCCDEALPPHTVDCPECGCR
                     ADVPRLDSGEAAFCPRCGHKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1130 a         1258 c         1407 g         1201 t
ORIGIN      
        1 aacaacttgg gtgcggataa acctttgttt atctaaaccc ctgttttctc aaaaattgat
       61 tcaaatcttc ggcaaatttc cgatagcctt tgccgttggc gtggatttgg tcggatttca
      121 gattattatc gcccaaaatt tccgcccacg cgccgccgaa cagcggaatg ccgtattcct
      181 cggacaaatc ctcatacagc ggatgatcgt tcaaatgccc gaacaacgcg cccaaggtaa
      241 tgtgcggcac gccgacgagg acggcgggga tgttttcctt ctgcacggtt tcgatgattt
      301 tcgcgatatt ggcgcgggtc tgctccttgg gaactttgcg cagaaagtcg ttgccgccta
      361 tgccgacaat cacaagcttg ggtttgcgtg ccaacagcgc gggcaggcgc gacagggctt
      421 gggcggatgt atcgcccgat acgccgccgt tgacaatatt ccaacccgtc agtttttgca
      481 gttgcgcggg gtaggattcg ccagggtttg cgccgtagcc gaaggtaagc gaatcgccca
      541 aggcaagtac ggtgcttcct tcgggaattt tggcgtgggt tcgggcggat tttctgccgc
      601 aggcggtaag gagcaacgcg cctgcgccga ggaggaaggt tcttctgttc attggttttt
      661 cagatggcat cgagccattt cgggtggtat ttcagggttt cacggtaaac cggacagctt
      721 tcggcgtgcg cgcctttgag gtagccggta ctcatcagaa attcgccgac gatttcgccg
      781 ccgacgaatt tgaaatgttt tttaaagagt ttaacccatt cgtctttgct tcgcggatgg
      841 tgcgcgtcga gccagttctt gaacgagccg tattcttgtt gcaacgcttg gatttgccgt
      901 gcattgaaaa tggcggcatc gattttcagg cggttgcgga cgatgcccgc gtcggcaagc
      961 aggcgttcgc ggtcggtgtc gtcgaaggcg gcaacggtat tgatgtcgaa accttcaaat
     1021 gccgtctgaa acgcctgccg cttcttcagc atcagcgtcc agcttaatcc tgcctgattg
     1081 atttccaaca ccagccgttc aaacagtccg ttgtcgctgt caatcggaaa accgtattgc
     1141 gtgtcgtggt aatgtttgtt cgggttatcg gtgttttcgg gaagtgaggc ggcaaattcg
     1201 caatagttca cggtttactc gaccatctgt tgcgccgcct gtttcagtcc ctgttcgagt
     1261 gcggcggtca tcgcggcgta gccgtcaccc tgctgttcgg tttcgatatg gaagggtctg
     1321 ttcgtaccgt cgggtaggac ggcgtagccg ctgatgaggg ttttgcccgt gtagctgcct
     1381 tggaatgcgt cgatatagac cgtccatttt tcggtactgc cgctgcgtga ggcaggaaca
     1441 aagatgcgtg tgttgtccaa acggttgaat gcattgctca acgccgcttc gagcatatcg
     1501 tccaaggtgt ctgcccaaac gtggttttgt gcggtgttga ggcggtaggg gtcggtttga
     1561 tagaccagtc cgccgcgttt gagcggttcg gcaagacgga cttcgacggc ggtttcgccg
     1621 ccttgtgttg caggacggat gtagcggctg tcgggcaaca cgaaatattg tgtgctttgc
     1681 acagtaccgc aggcggcaag cgtcagggcg gcggcaatcg ggaagaggcg cattatcggc
     1741 ttcctttcgg gatggggtct ttgctgctgc tgttgaaaat cagcgcgttg ggtttttctt
     1801 tcaaagtgtt aatgacgggt tgaacgtctt ttaaggtttt gtccaaactt tgcaacgtat
     1861 tttgtacgtc gccgtagata ggcgattgcg gcgatacgcc ttgcagggtt gtgcgcaact
     1921 ctttcagggt ttggttcagt tcgttcggaa tgttttgtgt ctgcggtttg ccgaccagtt
     1981 tgtcgatgga gcttagggcg gcattggcag atttgagtgt ggatttgagc tcggcaagcg
     2041 aaccgttcaa ttcggcaacc gtcttattta aaggcagttt gccgaatttg tccagcaaat
     2101 ccgccaattt gacctgcaaa tcgtccaaac cgccgccctg ggtcgcgata acggtatcgc
     2161 ctgcataaac ggtatgcggt cgcagcttag gtgatgcgga aggctgatcg ttcaactcaa
     2221 tcattttgct tccggtcagc aggttgttgc tggagatggt ggcggtcagg cctttgttta
     2281 aggccgtctg aaattgttgt ttccaatgtt ctttgctttg ttcgtcggca ttgatttcca
     2341 aacgggaagg ttcaatgcgg atgcgtacgg gtatccagcc gttttcaaac aggtgcaggc
     2401 tgtcgttgcg gtcgaaataa ggaacgtcgg aaaccacgcc gacattcagc cctttgtact
     2461 cgacgggcga accgacggtc aggccgcgca cggattgttt gaaaaacgcg gtgtagtaca
     2521 gcgagcggtc gtcaggcagg ttggcgactt cgctgcggct gtcgtaaagc gtgaagctgt
     2581 cttcgctttt gacgttttta ctgtttttgg ttttcggcga atcaaatgaa atcgcgcccg
     2641 acagcagggc aggcagaggg gcggaattga gtttgatgcc gctgcctgtg gtttcgatat
     2701 tgatgccgct ttccagccag aaacggctgg cggaatgaat cagtttgtcg ttggggcttt
     2761 ggatgaagat ggtgtaatgc acgctttggt cggacgggtc gaaatgcgcg ctttcgactt
     2821 gcccgaccat aaagttttca tacaaaacag ggctgttgac gttgaggatg cggtcgtttt
     2881 taccaatcaa attcaagcgc agcccgcttt gcccgatggc ggtaacgggc ggaatgtcct
     2941 gcacttggaa cacgtctttt gcctcgtcgc ttttgccggg tgtaaaggcg atgtacgaac
     3001 ccgaaagcag cgtacccaaa ccggttacgc cgctttggtc gatacgcggc ttgaccaccc
     3061 aaaactgggt atcgctgcgg atgaggccgg atacgtccgc attgagttgg gcggttactt
     3121 ccacgccttt ttggtcgtcg cgcagtttga ttcgggtaac gcgtccgaca tcgatgctca
     3181 atactttgat gaccgtattg ttgacctcaa tgccttccgc gctgtccatc aagagcgtaa
     3241 ccacaggccc cctgttgcgg atttccttaa cccaaagcca gccgccggca atcagcgcga
     3301 tcagcggaac cagccagacg gcagagagga aggtgttgtt tttgcggacg cgtgcttggg
     3361 cgtgtccgtt tggaggaggg ctgttgtcag tcatgttttt ccgtttcatt gaaagcaatg
     3421 ccgtctgaag cgcgtttgtc ccaaagcagg cgcgggtcga aataataggc ggacagcatc
     3481 gtcagaatca cgaccaggca gaaatagact gccgcactgc ccggaatgac gcgcgcggca
     3541 taagtgtgga acgaacacat caaaataata atcacaaaaa tatcaatcat cgaccagcgg
     3601 ccgaccgctt cggtgatgcg gtagaggtgc gacaatttct ttgcacccgt tggcaaagcg
     3661 aagcgggcgg acgcaatcaa aaccgacatt gccgcaatct tcagtaccgg caccaaaata
     3721 ctcgcgctga aaataaccgc cgcaatcagc ctgtcgccct cgtcccacat ataagcgatg
     3781 ccgttaagga tggtattgac ctccgtggcg gcaggattgg acgaaatcat aatcggcagg
     3841 atattggcag ggaaatacaa aataaccgcc gccgtcagaa acgccgacga aatactcaga
     3901 cttttcggcc gtcggcggta cagttccgca ccgcacacgc cgcaggggga ttcggcactg
     3961 tcgcggaaat acaggcagcg gctgcaacag gttttacctt ccgatgccgt ctgaaccgca
     4021 ttatcccccg tcagccgccc gatttgaaaa tacacccaat gctggggaac cgataccgaa
     4081 gtccgaatca gcataaccga cagcgcgaac atcagataaa acgccggccc gaagcgaacc
     4141 tctgccacag acgagagctt gatatacgcc accaaagtgg aaacaaaaaa cacatccacc
     4201 atcatcgcct gtctcaagcg caccatcaca cgcgttgcca aacgcagcgc aggatacgcc
     4261 tgtttccgta tcagcgcggc atagacatac aggcacagca gcagaaacag aaccggcgcg
     4321 ccgaaagtca gcacaaacat cacttcggcc aaaaaaccat aatcctgaaa caccatcagg
     4381 cgcatcatct cgggcagcga aaggacggat gccgcacccg gtatcccgac ctcgatatac
     4441 gtcataccgt aagcaaacgc cattaaaatc agcgaagccg ccgcataggc gggcggggcg
     4501 gaaaaaggat gcctgcccac cctgaagagt ttgtgtccgc aacggggaca gaacgccgct
     4561 tctccactgt ccaaccgggg tacatccgcg cggcagccgc attccggaca atctaccgta
     4621 tgcggcggca gggcttcgtc gcagcagttg ccgcgaaggc tgtgggggat attttcggca
     4681 aacggcttca tcgggaaagt ggcaaatata agtggcggca ttataaagga aaaaaggcga
     4741 tacacccaat caggatgcaa aaccggcagg ttcggcagta tgaacaaacc cttggtgtcc
     4801 ggcaaaaata ttttttgagc ggttgtccgc cgactgccga acccatttga taaaatcccg
     4861 attctgtaaa gaatagtaaa atacctgctt aaaaacatca cgcaaatgat attttttcca
     4921 aaaatatgtg ataataaatt tcgtttatga ttctttatta tttttatagt ggattaacaa
     4981 aaatcaggac aaggcg
//
LOCUS       DAXHMX010000142         4876 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHMX010000142 DAXHMX010000000
VERSION     DAXHMX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.142"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(92..574)
                     /locus_tag="ACFM4S_001810"
     CDS             complement(92..574)
                     /locus_tag="ACFM4S_001810"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6051440.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(727..888)
                     /locus_tag="ACFM4S_001811"
     CDS             complement(727..888)
                     /locus_tag="ACFM4S_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051441.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="ACFM4S_001812"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="ACFM4S_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6051442.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQTA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1498..2208)
                     /locus_tag="ACFM4S_001813"
     CDS             complement(1498..2208)
                     /locus_tag="ACFM4S_001813"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6051443.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            2615..3880
                     /locus_tag="ACFM4S_001814"
     CDS             2615..3880
                     /locus_tag="ACFM4S_001814"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6051444.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     ASYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3937..4743)
                     /locus_tag="ACFM4S_001815"
     CDS             complement(3937..4743)
                     /locus_tag="ACFM4S_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6051445.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1050 a         1107 c         1435 g         1284 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacatg tcttcagagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgaccgca accatcgtga
      241 agtagcagct gttggtgtgg cggatttcgc cggtgcggat gttttgtgct tcgacacgga
      301 tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat
      361 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtggga aggcagttgg cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt
      601 tttgaaatgg gtgcggggaa attttaggga atggggaagt ggaattcaag ctaaaaaaag
      661 ctatgatggg atagtctgcc gaaatggacc gccgatgccg tctgaagcct tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac caggaaaaac acgagtgcca atacggcgac
      781 ccaaacgacg agcatgaatc cggcaactgc ccgtcccata cggtaaaccg ggtttttgcg
      841 ttcgtcttcg atgggggagg tgtggtgttt ttcgggttgt ccggacattt tgttctcaat
      901 cggaaaagtt ggaaacgggc gtaaggttat gttgccgtgc aaaaagattc aagaaggaag
      961 gtcagcagcc gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
     1021 cggcatcgaa tccgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttaaggtcgg
     1081 cggcggggtg tttgaatttt ttacgcaaca cgtctatggc ggtctgtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcttcttcgc
     1321 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcgt
     1441 ggggcaggtt tcagacggca tgggaaacgg attccgatgc cgtctgaaca gattgtttta
     1501 ttcttcgtct ttatttttct gcggttgcag gttttcgtaa atttcgggca gcgagccgat
     1561 aatccagtcg gggcgggtcg catcgtcttg cgagagcagc gtcatatcgc cgtaaccgaa
     1621 ggtaacgccg acgctcaggc agccggcggc tttggcggcg atgatgtcgt tgcgcgagtc
     1681 gccgaccata accatgtttg caacatcgat acccaaaact tcggcggcat gccgcagcgg
     1741 cagggggctg ggttttttct cgggcaggct gtcgccgccg agtatcaggc tgaagtagtc
     1801 ggcaagtccg agctgtttca acagttcgac ggcaaggatt tcgtttttgt tggtaatgac
     1861 tgccagcggg atgcccaaag atttgagcaa tgccagcccg gcttcggttt cgggataggg
     1921 gcgggtgaag acgctcaaat ggtcgcggta gtatttcata taggatacga aacctttttc
     1981 ccacagttcg gaatcggctt cgcggtcgcg gtcgttggtg aggacgcggt gaaccagttt
     2041 gccgatgccg tcgcccacat agctttcgac cacttttgca ggcagcggtt tcataccgag
     2101 ttgctccaac atcgcttctg cggcggcggc aaggtcgggg acggaatcgc acagtgtgcc
     2161 gtccaaatcg aaggcgacgg cttggacgtg ttcgatggct gcattcataa tctgatttcc
     2221 tgtcgggttg aaagggttta ttttaacatc gtttgcacag ggcttctaat gtatagattg
     2281 ttaacaatcg caacaggttt tgcaggcggt gttttcggat ttcggggcgg aatcgggcaa
     2341 gggtttgaaa tcccggcgat gttccaatta aatttttaaa aaataaaagg atgcctatat
     2401 ctttatttaa tttgacattt atttttcgga cgcgcgcgct gctttttcag gcggagtccg
     2461 ggctgattcc gtgcggcttg aaaaataaaa tcatatatag attaatcaaa tcgggcttat
     2521 gccgactaca cgctattaca ttttgtcggg aaattggtta tagtaatgag cgaaatgcac
     2581 gcccctgaaa atctttttgt gaaaaggaag caaaatgtct gaagcccttg caaaagaaac
     2641 cctcaatccg tttgaaatcg cgcgcaaaca ggtcaaaacc gcctgcgacc ggctgaaaac
     2701 cgatccggcc gtttatgaaa tcctgaagag tccgacccgc gtgttggaag tcaatttccc
     2761 cgtcaaactc gacgacggca cggtcaaaac cttcacgggc taccgttcgc aacacaacaa
     2821 cgccgtcggc ccctataaag gcggcgtgcg cttccatccc ggtgtgaatc tggacgaagt
     2881 caaagccctg tcaatttgga tgaccatcaa atgctgcgtg gccggcattc cttacggcgg
     2941 cggcaaaggc ggcgttacct tggatccgcg cgattattcc gaggcggaac tggaacgcat
     3001 cgcccgcgct tattccgaag ccatttctcc gctcatcggc gaaaaaatcg atattcccgc
     3061 ccccgatgtg aataccaacg gcaaaattat gtcgtggatg gtggatgctt atgaaaacgt
     3121 ggtgaaacat tccgcgccgg gcgtatttac cggaaaaccg gttgagttcg gcggctcttt
     3181 ggcgcgtacc gaggctaccg gttacggcgt gaacctcgcc gccgtccaag ctttggaaaa
     3241 actgggcaag gacgtgaaag gcgcgactta cgccatccaa ggcttcggta atgtcggtta
     3301 ccacacaggt tattacgcgc atcaatccgg cgcgaaagtt gttgcggttt ctactgttga
     3361 cgttgccatc tacaatgaaa atggcttgga tatggaagcc ctgtttaaag agttccaaga
     3421 aaaaggcttc atcaccaacg aagccagtta cggcaaagaa attaccaacg ccgaactttt
     3481 ggctttggat gtggacgtac tcgccccttg cgcgttggaa aaccaactga cttccgaaaa
     3541 cgccggtaaa gtccgcgcga aaatcgtggt cgaaggcgcg aacggcccga ctacgcccga
     3601 agccgacgtt atcctgcgtc aaaacggcgt attggtcgtg cctgatattt tggcgaactg
     3661 cggcggcgtg gtggtatctt attttgaatg ggtacaaaat ctgcaaggct actactggga
     3721 gtttgacgaa gttcaggaaa aagaaaccgt cgtcctgcgc cgtgcgttcc gcgatatatg
     3781 gaacttggcg caagagtatg atgtcgattt gcgaaccgcg tcttacatga tgagtatccg
     3841 ccgtgttgaa aaagcgatga aactgcgcgg ttggtattga tgtttgaaca aaaaatgccg
     3901 tctgaagcct tcagacggca ttttgtgcgg gcgggattaa tggtgcgaat ggccgatttg
     3961 gaaggttaaa gtcgagtagt tcgcctgttt ttggcacacg ctttgatcgg ggaagtcggc
     4021 tttgtgttcg acattggctt tccagaagcc ttggcgcaag gggatgatgt ccacttcgcc
     4081 tttgtcgtct gtgctgtcgg agaaagcctg tgcttcggtt ttgtgcgttt tgctgcggtc
     4141 gctggtgtcg aagccgtcaa aggtggcggt aacggtggca ttgggcagcg gttcgccacg
     4201 gaacagaacg cggactttga agcgttcgcc tacgtgaatg ttggcgggat tgtccagcgg
     4261 gacgatttcc aagttttgtc cgaccggttt ggtgatgatg gcggtgtccg cgctttcgtg
     4321 tccgacgttg acgatgtttt tgccgaacat tcgggtttgt tcgcaatagc ttgcgtcagg
     4381 catttctttg atgcccgcct gtttccagcc tgctttgttt tttgaccaga aagtaggctg
     4441 atattcggcg atgacgaggt aactgccgtc cttaacggga cggttgcttc ggtactggta
     4501 gttgtatgtg ccgcgttgaa tcatgttttc cttgcctttt tcggtaacca gctgcatcgg
     4561 tttgctgaag atgtgcaggc ggtctttggc gatgggttcg agttcgggaa attcgccgta
     4621 gcccaagtcg gctttaaggt attcgccgcc gtgcgtgtgg gcggtttcga cccagacgcg
     4681 gtgggcgtgg gcggatgtgg caaatagggc ggaaacggcg agcagtgtca atgttttctt
     4741 catggtgcag cctttgtctc gtgtgggtta aagattttgt tatgttataa caaaataggt
     4801 atcgtgtgaa gacggatttg ctgtctgaaa actgtctttt tgtatagtgg attaacaaaa
     4861 accagtacgg cgttgc
//
LOCUS       DAXHMX010000143         4860 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHMX010000143 DAXHMX010000000
VERSION     DAXHMX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.143"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(209..937)
                     /gene="rmpM"
                     /locus_tag="ACFM4S_001816"
     CDS             complement(209..937)
                     /gene="rmpM"
                     /locus_tag="ACFM4S_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6051446.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1301..2251
                     /locus_tag="ACFM4S_001817"
     CDS             1301..2251
                     /locus_tag="ACFM4S_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6051447.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2364..3098)
                     /gene="fnr"
                     /locus_tag="ACFM4S_001818"
     CDS             complement(2364..3098)
                     /gene="fnr"
                     /locus_tag="ACFM4S_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6051448.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3301..4722
                     /gene="hemN"
                     /locus_tag="ACFM4S_001819"
     CDS             3301..4722
                     /gene="hemN"
                     /locus_tag="ACFM4S_001819"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6051449.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1239 a         1329 c         1177 g         1115 t
ORIGIN      
        1 cggcaatggg acacaaacct gaatctgaat ccgaacctgc accccgattc tgacgatagc
       61 cgtttcagaa aaccacccaa aaaaacaaac cccgcaaaag cgggggtttg tttcacatca
      121 tcgatgccga ccgacgtttc acgaatattg caacagtacc tggtacaaaa tccactaacc
      181 tcatgcatcg gcaagatatt gcctagcctt agtgttggtg atgattgtgt gccggcacaa
      241 cctgacgggt tacgatgctg cggattttca catccacacg gcggtcaggt tcgatacatg
      301 caatcagagc ctcacgtttt ttggctttag agactttcgc acccagtttg gcaacttcgg
      361 cttcacaaac ttgagtcatt tgcgcttgag attcgcccaa gccgacagca gaaattctag
      421 aaacaggtac gccgttgctg accaggttgt ttgccactac gtatgcgcgg cgttcggaca
      481 gggcctgatt gtatttgtca gaacccataa agtcggtatg gccttcgacg cggacagatt
      541 ggatattggt ttgacccagg cgttgcgcca atactttcag gttgtcttga gcttcggcgc
      601 gcaatgaatc cttatcgaaa ccgaacaggg ttttggcaga cagggaaatg gtttcatcaa
      661 catattgcgg agcctgctcc acaacgacga caggagcggg tgcgggttcg ggttctggct
      721 cgggttcggg ggcagcaacc gcatcgccgc attctacgcg accttggctt gctttatcaa
      781 agtaggcgtt tttccagcat tcgccatagt tgttgcgtac gatttcgttc gactggccgc
      841 ttacggtgta accctgaacg gacgcctcgc ccgcaacagc agtgccggaa gcgagcaatg
      901 caacgaataa tgcgcttaat ttcagctgtt tggtcatttt attccctcat taaatttgta
      961 cagcagatac agaagcaact gctgaattgc caacatacag gccgctgtag aaacatggcg
     1021 gcaaatcgga tatttcagtc tccactataa agaagcaggg ggaggactgt caatactcaa
     1081 cgcctgaaaa caaccgtaaa acagcctgtt tcctgatagt ctatcatgcc gaaaaatcaa
     1141 ttccgtgtca ttactttagg gggatttttt ccatatcgca cgattgccgt tgcacaaaaa
     1201 caacaaacac aacggcaaag ccccataccg taccggcaag aaaatatgat aaattataaa
     1261 caatgttcag ccacccgaca cagacccaca ccgacccgcc atgaaattac aacaattgaa
     1321 atacgcctta gaagtttacc agcacaacct gaacgtttcc gaagcggccg aagccttgtt
     1381 tacttcgcaa cccggcatct ccaaacaaat caaattgctg gaagaagaaa tcggcattca
     1441 gatttttatc cgcagcggca agcgcgtggt ttcggtctcg cagccgggca aggtggtttt
     1501 ggatattgcg gaacgtattt tacgcgatgt acagaacatt aaaaatatcg gcagcgagtt
     1561 taccggacag gacagcggtt cgctgacggt tgccacgacg catactcagg cacgctatgc
     1621 cctacccttg attgttgccg attttgtgaa acgctatccg aaagtcaacc tgaccatcaa
     1681 acaggggagc cctgccgcca tcgcacgtat ggttacctca ggcgaggcgg acttggcgat
     1741 tgttacggaa cggatagacg accatcccga actgggaaaa cttccctgct atgactggac
     1801 tcatgcggtt atcgtaccga acgaccaccc cttgctcgaa tgcagaaacc ccctccgcat
     1861 tgaagatttg gcgaggtttc cgctgattac ttatgaattt gcattcaatg cgggcagcag
     1921 catcgcgcgg gcattttcca aagcccgttt ggaacagccg gatgtcgcat tggcggcggc
     1981 ggatacggac gtattgaaga cttatgtgcg cttgggtttg ggcgtgggac tgatggcgaa
     2041 aatggcgtac aacccggata cggacggcga tttgcagctt gtggatgcgg cacacctgtt
     2101 cgagccgtcg ccgacgtgga ttgctttgcg cagcgatact tatttgcgcg gatatgccta
     2161 cgactttatc caagcgtttg cgccgcacct gacacgcgag aaggtggata ggattctgta
     2221 cacgcccatc agcgaggatt tttcgattta ggcggctgcc ggttttcaga cggcactttg
     2281 cggcagatac aacaaacagg acagatgttt tcgtctgccc tgtgtttatt gagaatgccg
     2341 tctgaaatgt tcgtacgggt taatcaaatg gcgtgcgaac aaccggatac catcttcttc
     2401 aacacctgca gattgaggat cttgatgtgt ttgtgttcga cggaaatcaa tccttcctga
     2461 tgaaatttag ataatgtgcg gctgacggtt tcaagtttca gcccgagata actgccgatt
     2521 tcttcgcggg acattcttaa gatgaagtcg ttggcagcaa aacctcggga ataaaggcgt
     2581 tgggaaaggt tcaacagaaa ggcggcaatc cgctcttcgg cgcgcatatt gcccaacagc
     2641 agcataacac cttggtcgcg cacgatttca cgactcatca tgcggaaaaa gtgggtacgc
     2701 aggctgggga tattctgccc cagttcttcg atgtgggtaa acggcagttc gcacacttcg
     2761 ctgtcttcca aggcgaccgc gtcgcaactg tgcacatggg aacagatgcc gtccatgccg
     2821 atgagttcgc ccgacataaa gaaacccgtt acctgatcgc ggccgtcctg actggcgacg
     2881 gttgttttga agaagcccga acggatggca aagagcgagg taaaggcttc gccgacacag
     2941 aacaggtatt cgcccttttt caggcggcgg ctttgacgga tgacggcatc gagttggctg
     3001 agctcgttgg gcagcagccc gacaggcagg cagagttccc gcaaagaaca ggaagaacac
     3061 agcgttttca tctgatgtgt agtattatgc gaagccatac cgtacctttt tgtgcgcttt
     3121 gccccatcat gattatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgtcc
     3181 ttatttaaat ttaatccact atatgtgctt attgacacat atcaagacag gtttatcata
     3241 ctgtggcatt ctaccaaact atccaaaatt tgactaacat cataaaaccg cacacacacc
     3301 atgaaaatca ttcagataca gaacaatcac aatgtaaacg atgaccgccc cgagtttgac
     3361 cgcgcgctga ttgccagcct gcccgccagc ggcccgcgct acacttccta ccctaccgcc
     3421 gaccgtttcc atgacggttt ccgcgaaggc gaatatatca aagctttaca tttgcgcggt
     3481 atgggcgcgt taaacaaacc gctttccctt tacattcaca ttccgttctg caacaccatc
     3541 tgctactact gcggctgcaa caaaatcatc accaaagaca aaagccgcgc cgatgcctac
     3601 atcgaatatc ttgaaaaaga aatggaactg ctcgctccac atctgaacgg acggcaccag
     3661 cttgcccaac tgcacttcgg cggcggcacg ccgacctttt tgagcgacga acagatcgaa
     3721 cgtgtcttcc gcatgatacg caaacatttc gagttaatcc ccaccggcga atactccatc
     3781 gaaatcgacc cgcgcaaagt cagccgcgac accgtcctca tgctcggcag actcggcttc
     3841 aaccgcatga gcatcggcat tcaggatttc gaccccaaag tgcaggcggc ggtcaaccgc
     3901 atccaaagtt acgaagaaac caaagaagtc atcgatgcgg cgcgcgaagc ggggttcaaa
     3961 tccgtcagcg tcgatttgat ttacggcctg ccgcaccaga cttcggaaag catcaaaacc
     4021 accatcgata ccgttttgtc gctcgatccc gaccgcctcg ccctttatca ctacgcccac
     4081 ctgccgcacg tgttcaaacc gcaacgccgc atcgataccg ccgccgttcc cgacagcgaa
     4141 gagaagctcg atatgctgca atactgcgtc caaaccctaa ccgaacgcgg ttacgtcttc
     4201 atcggcatgg accatttcgc caaacctgac gacgaactct ccatcgccct caaagaaggc
     4261 ttcctccagc gcaacttcca aggctattcg acctacgcgg attgcgattt ggtcgccatc
     4321 ggcgtgtcgt ccatcggcaa aatcggcagc acctattccc aaaacgaacg cgacatcgat
     4381 gcctactatg ccgccatcga cgaaggcaga ctgcccatca tgcgcggtta tcagctcaat
     4441 caggacgaca tcctgcgccg caacatcatt caggatttga tgtgccgctt cgcgctcgac
     4501 tatcggattt acgaaagtat gttcggcatc ccgttcgacc gctacttcaa agacgaactg
     4561 gcggatttgg aaaaactcgc cggtttggga ttggtgcgcc tgaacagcca cgggctgacc
     4621 gttaccccga aaggacgctt cctcatccgc aacatcgcca tggtcttcga ttaccacctg
     4681 cgccacaaag aaaccaaggc gaaatattcg caaacggtgt gattgcgtct aacgtacaaa
     4741 tgccgtctga aaggcttttt cagacggcat tttgctgccg gcaggataag tgttttcaag
     4801 aacaggcggc ggcatatcat aacgttccgc acctttgtgt ccgaccgttc cgaaaccaag
//
LOCUS       DAXHMX010000144         4774 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHMX010000144 DAXHMX010000000
VERSION     DAXHMX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.144"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..857)
                     /locus_tag="ACFM4S_001820"
     CDS             complement(<1..857)
                     /locus_tag="ACFM4S_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6051450.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTLSHKIDQRAFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDEEGNWSDVGLSSASFLGGLMGFQWGAALGVGAFGLLTGGAGWLVVGT
                     IIGGGSGAWWGSLGAEKAWRWFEDFLNLNRSDKFHVTDPLALDLDGDSIETVATKGLA
                     GALFDHRSQG"
     gene            2035..4263
                     /locus_tag="ACFM4S_001821"
     CDS             2035..4263
                     /locus_tag="ACFM4S_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFC6051451.1"
                     /translation="MCSAVLFIYFFLLNIFLFQPAVIHIQSNYILFMSIVSAPLPALS
                     ALIILAHYHGIAANPADIQHEFCTSAQSDLNETQWLLAAKSLGLKAKVVRQPVKRLAM
                     ATLPALVWCDDGNHFILAKTDGGGEHAQYLIQDLTTNKSAVLSFAEFSNRYSGKLILV
                     ASRASVLGSLAKFDFTWFIPAVIKYRRLFFEVLVVSVVLQLFALITPLFFQVVMDKVL
                     VHRGFSTLDVVSVALLVVSLFEIVLGGLRTYLFAHTTSRIDVELGARLFRHLLSLPLS
                     YFEHRRVGDTVARVRELEQIRNFLTGQALTSVLDLAFSFIFLAVMWYYSSTLTWVVLA
                     SLPAYAFWSAFISPILRTRLNDKFARNADNQSFLVESITAVGTVKAMAVEPQMTQRWD
                     NQLAAYVASGFRVTKLAVVGQQGVQLIQKLVTVATLWIGARLVIEGKLTVGQLIAFNM
                     LSGQVAAPVIRLAQLWQDFQQVGISVARLGDILNAPTENASSHLALPDIRGEITFEHV
                     DFRYKADGRLILQDLNLRIRAGEVLGIVGRSGSGKSTLTKLVQRLYVPEQGRVLVDGN
                     DLALAAPAWLRRQVGVVLQENVLLNRSIRDNIALTDTGMPLERIIEAAKLAGAHEFIM
                     ELPEGYGTVVGEQGAGLSGGQRQRIAIARALITNPRILIFDEATSALDYESERAIMQN
                     MQAICANRTVLIIAHRLSTVKTAHRIIAMDKGRIVEAGTQQELLAKPNGYYRYLYDLQ
                     NG"
     gene            4487..>4774
                     /locus_tag="ACFM4S_001822"
     CDS             4487..>4774
                     /locus_tag="ACFM4S_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6051452.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDEL"
BASE COUNT         1226 a         1089 c         1073 g         1386 t
ORIGIN      
        1 ccttggctgc ggtggtcgaa caatgcacct gcaaggcctt tggtggcaac ggtttctata
       61 ctgtcgccat ctagatctag ggcaaggggg tcggttacgt ggaatttgtc gctgcggtta
      121 agatttaaga aatcttcaaa ccatctccaa gctttttcag ctcctaatga tccccaccaa
      181 gctcctgagc cacctccaat tatagttcca actactagcc aacctgcgcc accagtaagt
      241 aaaccaaaag ccccaacacc caaagccgca ccccattgga agcccattag cccacccaaa
      301 aaagaagctg atgagagacc aacatcactc caatttcctt cttcatccaa ggctttccac
      361 caagcacttc ccatactcaa agttcccatg gcaacagttg ccccataagt aaacttaccc
      421 aaattcgctg ccataacttt tgattttgaa gcattctcta acgctgttgc cgcacgtctt
      481 gcatattgct gcttgttata gttgctagca ttcaatgctt tttgcaaatc actttgtact
      541 tttcttgcaa caaatgcctc ctttgaatta ccacgaaatt tactagcagc tcttccaatt
      601 ccttctttta agcttttttg tgcctcttct gctgtgcgtt ggaattctct tgccttagca
      661 gacagacgct caccctccct taaaaatgct cgttgatcta tcttgtgcga aagggtcaaa
      721 tattcttcgt acattgagtt aatcgtacca atcgccctaa ccacattttc atcagaaaat
      781 atggaaataa tagcatccct atacgcacct agagtaatat tgtttctatt attagttata
      841 gcattattcg aatacataat agcacctcca aacttagaac tgaatacgtt tatattctct
      901 gtgtaataat atcaaataat taattaatgg ataagtaagt ccgaaaaaat aacaactaaa
      961 ataagcaata tactgattct ctcctttatt aattagatat attgaaaaac aaataaaaaa
     1021 taaacagaaa acattcatgt gaaaaagcca cctcatattc ttatttataa gtttaaaata
     1081 actaggcatt gtatatttaa aaatcggtac atccaacgga aacaaaccac ataaaaattt
     1141 ttctttattc tttattaaag acatagtttt atttttcaaa attagtccag ataatatact
     1201 aacaattact attaaaacaa taagcacagc gtaatttttt ataaaattca ttaaaataat
     1261 aacgaaaata ctaaatagat tacatactgt aaattgaaat ataaaatatt cagtatttaa
     1321 tccattttca accgcgagtt ttttaaaata tccttgctcg aaaaaattaa aaaaatccat
     1381 taatactttc ctctcaagaa aactaatttt agtttagtta agataaaatg caatattacc
     1441 ttttctccat aaaagtatgg agcttatata cataaattat gtgcgaggta gcctgaaaaa
     1501 tatccaatac aagatagtag gagatgtttc ccttatcact attggccaat acccaattgg
     1561 caggcactct atttccaaaa ttttctcaag aatagttaaa tattattaat tagtttaaaa
     1621 acaataacat acgtttgtat ttatccgttt tggtctgcct gaaacagact ctatgtccat
     1681 acacggacat gtagacagtt tccttgttat tttggttgct cgtcattagt gatgatactt
     1741 tactcctctc ttaaaaaatc gtcaagaact gttatcaatc tttaaagtct tttttaaaca
     1801 aatatatata cgttcaggct gtctgaaaca caaaaaaatg atgttacccg accgtgacag
     1861 agtcggggca gccggtacct taaccctgca ccttaaccct gtgccttaac ggtatgcagg
     1921 aggcacgaga ggtgcgaggc atcctcatct aactcgattc ctaagccgtt ttaccttcct
     1981 gaaacgagaa cttttccata ctttctcttt acaattccca tatacatcct tacaatgtgt
     2041 tctgctgttt tatttatata tttctttcta ctgaatatat tccttttcca gccagccgtt
     2101 atccacatcc aatctaacta tattttattt atgtctatcg tatccgcacc gctccccgcc
     2161 ctttccgccc tcatcatcct cgcccattac cacggcattg ccgccaatcc tgccgatata
     2221 cagcatgaat tttgtacttc cgcacagagc gatttaaatg aaacgcaatg gcttttagct
     2281 gccaaatctt tgggattgaa ggcaaaggta gtccgccagc ctgtcaaacg tttggctatg
     2341 gcgactttac ccgcattggt atggtgtgat gacggcaacc attttatttt ggctaaaaca
     2401 gacggtgggg gtgagcatgc ccaatatcta atacaggatt taactacgaa taagtctgcg
     2461 gtattgtctt ttgccgaatt ttctaacaga tattcgggca aactgatatt ggttgcttcc
     2521 cgcgcttcgg tattgggcag tttggcaaag tttgacttta cctggtttat tccggcggta
     2581 atcaaatacc gccggttgtt ttttgaagta ttggtggtgt cggtggtgtt gcagctgttt
     2641 gcgctgatta cgcctctgtt tttccaagtg gtgatggaca aggtgctggt acatcgggga
     2701 ttctctactt tggatgtggt gtcggtggct ttgttggtgg tgtcgctgtt tgagattgtg
     2761 ttgggcggtt tgcggacgta tctgtttgca catacgactt cacgtattga tgtggaattg
     2821 ggcgcgcgtt tgttccggca tctgctttcc ctgcctttat cctatttcga gcacagacga
     2881 gtgggtgata cggtggctcg ggtgcgggaa ttggagcaga ttcgcaattt cctgaccggt
     2941 caggcgctga cttcggtgtt ggatttggcg ttttcgttta tctttctggc ggtgatgtgg
     3001 tattacagct ccactctgac ttgggtggta ttggcttcgt tgcctgccta tgcgttttgg
     3061 tcggcattta tcagtccgat actgcggacg cgtctgaacg ataagttcgc gcgcaatgca
     3121 gacaaccagt cgtttttagt agaaagcatc actgcggtgg gtacggtaaa ggcgatggcg
     3181 gtggagccgc agatgacgca gcgttgggac aatcagttgg cggcttatgt ggcttcggga
     3241 tttcgggtaa cgaagttggc ggtggtcggc cagcaggggg tgcagctgat tcagaagctg
     3301 gtgacggtgg cgacgttgtg gattggcgca cggctggtaa ttgagggcaa gctgacggtg
     3361 gggcagctga ttgcgtttaa tatgctctcg ggacaggtgg cggcgcctgt tatccgtttg
     3421 gcgcagttgt ggcaggattt ccagcaggtg gggatttcgg tggcgcgttt gggggatatt
     3481 ctgaatgcgc cgaccgagaa tgcgtcttcg catttggctt tgcccgatat ccggggggag
     3541 attacgttcg aacatgtcga tttccgctat aaggcggacg gcaggctgat tttgcaggat
     3601 ttgaacctgc ggattcgggc gggggaagtg ctggggattg tgggacgttc ggggtcgggc
     3661 aaatccacac tcaccaaatt ggtgcagcgt ctgtatgtac cggagcaggg acgggtgttg
     3721 gtggacggca acgatttggc tttggccgct cctgcctggc tgcggcggca ggtcggcgtg
     3781 gtcttgcagg agaatgtgct gctcaaccgc agcatacgcg acaatatcgc gctgacggat
     3841 acgggtatgc cgctggaacg cattatcgaa gcagccaaac tggcgggcgc acacgagttt
     3901 attatggagc tgccggaagg ctacggcacc gtggtgggcg aacaaggggc cggcttgtcg
     3961 ggcggacagc ggcagcgtat tgcgattgcc cgcgcgttaa tcaccaatcc gcgcattctg
     4021 atttttgatg aagccaccag cgcgctggat tatgaaagtg aacgagcgat tatgcagaac
     4081 atgcaggcca tttgcgccaa ccggacggtg ctgattatcg cccaccgtct gtccaccgtt
     4141 aaaacggcac accggatcat tgccatggat aaaggcagga ttgtggaagc gggaacacag
     4201 caggaattgc tggcgaagcc gaacggatat taccgctatc tgtatgattt acagaacggg
     4261 tagaaggcgg gggatgggcg tgtaggctgc ttttttcgta acgttctgcg ttttcagacc
     4321 gtcgtttact gggtttactg actgaaatga aaccaaagtg caggctgccg gaaagacttt
     4381 ttctgtcttt ttaggcagcc tgtacttctt tttcactcat ctagagacct ttgcaaaatt
     4441 ccccaaaatc ccctaaattc ccaccaagac atttagggga tttctcatga gcaccttctt
     4501 ccggcaaacc gcccaagcca tgattgccaa acacatcaac cgcttcccgc tattgaagtt
     4561 ggaccaggtg attgattggc agccgatcga gcagtacctg aaccgtcaaa aaaaccgtta
     4621 cctccgagac caccgcggcc gtcccgccta cccgctgttg tccatgttca aagccgttct
     4681 gctcggacaa tggcacagcc tctccgatcc cgaactcgaa cacagcctca tcacccgcat
     4741 cgacttcaac ctgttttgcc gtttcgacga actg
//
LOCUS       DAXHMX010000145         4749 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHMX010000145 DAXHMX010000000
VERSION     DAXHMX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.145"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..369
                     /locus_tag="ACFM4S_001823"
     CDS             <1..369
                     /locus_tag="ACFM4S_001823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051453.1"
                     /translation="GYDLSFGIRNIIIFFVKGIYTEDDSKPAGKKRPLLNCTPKVGHL
                     TDFRGCSFLWQNIQMNSDLPSFNTIWQGTADHLSISDSLVRRWVTKYRLHGESGIKRR
                     KHTTKYSVEYKLEAIRLVTG"
     gene            373..486
                     /locus_tag="ACFM4S_001824"
     CDS             373..486
                     /locus_tag="ACFM4S_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6051454.1"
                     /translation="MSQKAAADQLNLPDCSILLQWLRLYHLNGINGLKPKP"
     gene            806..1063
                     /locus_tag="ACFM4S_001825"
                     /pseudo
     CDS             806..1063
                     /locus_tag="ACFM4S_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1116..1298)
                     /locus_tag="ACFM4S_001826"
     CDS             complement(1116..1298)
                     /locus_tag="ACFM4S_001826"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6051455.1"
                     /translation="MKYTIFFSTSHHSNQTKATHRHTHVGSTAGQTTIRSGGDTTLKG
                     AQLIGIGIQADPQLQP"
     gene            complement(1449..2399)
                     /locus_tag="ACFM4S_001827"
     CDS             complement(1449..2399)
                     /locus_tag="ACFM4S_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6051456.1"
                     /translation="MYLGIDVSKLTIDCCLIVDGQNYQKKFQNNKGGFEQLINWLQSH
                     KVNDKLHCVCEATGTYYEALAEYLYSRYTITVENPRKIKGYAIAELQRSKTDTQDAKL
                     IAQYCQDRKHKLKAWKPPTKEQKQLQEIARYLDYLKQQRATEKAKQHEAPDYIKSHIQ
                     TTISNLTAQIQIVKKQLLQFYKDNPSYNNLRKRLKTITGIGEQATAVLLSTYKRHEFK
                     NARQFTAYLGLDPRKFQSGTSVNGKSRISKIGSSEIRKSLYMPALVAYRCNAFPEFVG
                     RLKNKGKHIKLILIAIMRKLAVIAFTILQNGQDFQVERYK"
     gene            complement(3025..4110)
                     /locus_tag="ACFM4S_001828"
     CDS             complement(3025..4110)
                     /locus_tag="ACFM4S_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC6051457.1"
                     /translation="MIYLFTGNMGTGKTSRVVSMILNNEDGLFKMKLEDGTEVDRPLY
                     FCHIDGLDKRQFKAHELTEEQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTQHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLD
                     NPAGVSGVEVASWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAIVGFVGWKSYGIF
                     KVYSKATDSRIEQEAQKESVVQTMTEQPASSEEMPLKNSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DEQQRTEQVEQSAKADKPQVLVMGGKP"
     gene            complement(4120..4410)
                     /locus_tag="ACFM4S_001829"
     CDS             complement(4120..4410)
                     /locus_tag="ACFM4S_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC6051458.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLIALEKFK
                     DYTSNAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(4411..>4749)
                     /locus_tag="ACFM4S_001830"
     CDS             complement(4411..>4749)
                     /locus_tag="ACFM4S_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6051459.1"
                     /translation="RHRKERKEGEDGGLLCDYFPEILACQEMGKPSDGMFHDISIPQV
                     IDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYQASYEPLCVFAEKIRFAVLLAFIIMSA
                     FVVFGSLKGK"
BASE COUNT         1182 a         1038 c         1037 g         1492 t
ORIGIN      
        1 ggatacgacc ttagttttgg tatccgtaat atcatcattt ttttcgtcaa gggaatatac
       61 acggaggatg attcaaaacc ggcaggcaaa aaaagaccgc ttctgaactg caccccaaaa
      121 gttggacacc ttaccgactt tagggggtgc agttttttat ggcaaaatat tcagatgaat
      181 tccgacttgc cgtcgttcaa tactatttgg cagggaacag cagaccatct ttctatttcc
      241 gattcattgg tacgcagatg ggtgacaaaa tacagattac acggagagag tggcatcaaa
      301 cgtagaaagc atacgacaaa atattcggtc gaatacaaac ttgaggcaat ccgcctggtg
      361 acggggtagg gaatgtccca aaaagctgcc gcagaccaac tgaatttgcc cgactgctcc
      421 atcttgctgc aatggttgcg cctctaccat ttgaatggta ttaacggttt aaagcccaaa
      481 ccctaaaccc aaaggaagaa agcccgtgaa aaaacagcat ccgccggaaa cgaaaaagcc
      541 gactatctga aaaccaagga agaactgctt gcggaattgg cttgccttaa agcggaaatg
      601 gctgccctaa aaaagctcga tgccttaatc tatgggaaag aagtgcggta gaaagaacgc
      661 aactcgtcgc agggttaagg caatgccatc cgttgaactg ctgttggtga ttgtcggact
      721 gccacgcggc accttctatt accaattggt tgtccaatcg gcagaagaca aatatgccga
      781 tttgaaacgg catatccatg atatttatca aaagcagttg aaagacaacg gtctggttca
      841 gagtatgtcc cgcaagggaa actgcttgga caatgcggca atggaaagtt tcttcggaac
      901 gttgaaatcg gaatgtttcc atacgtgcaa atatgattcc gttaccgaat tggaagctgt
      961 actgcacgaa tatatccgtt actacaacaa cgatagaatc aagttgaaat taaaaggact
     1021 gagccctgtt cagtacagaa ttcagtccct gaaagccgct tgattaaact gtccaacttt
     1081 tgggggtcag ttaatatcgg ttccaccaat agtcgtcagg gttgtagttg ggggtctgcc
     1141 tgtatgccta tgccgataag ctgagcacct ttgagggtgg tatccccgcc gcttcggatg
     1201 gtggtttgtc cggccgtact gccgacatgg gtgtggcggt gggttgcttt tgtctgattg
     1261 ctgtggtggc ttgttgaaaa aaagatagtg tatttcacgg tcaaataact ttacatttct
     1321 tgaagttgca agaatgcgca ccccgacttt ttcgggatgt ttgtttcgtt ttgaatggat
     1381 ttgatgtttt aatttatact ttattttcaa taaattgaaa atcaccggcg aaagcccagt
     1441 ttaatttttt atttatatct ttccacttgg aaatcttggc cgttttgcaa aatcgtaaac
     1501 gctattaccg ccagtttccg catgatggca attaatatca attttatatg cttcccttta
     1561 tttttcagac gccctacaaa ttcagggaag gcattacaac gatatgcaac aagtgcaggc
     1621 atataaaggc ttttccttat ttccgaactt cctatttttg atattctgct ttttccgttc
     1681 acgcttgttc ctgattgaaa ttttctaggg tctaggccta gataagccgt gaactgtctt
     1741 gcatttttaa attcatgtct tttataggtt gatagcaata ctgctgtcgc ttgctcgcct
     1801 atgcctgtta ttgttttcag ccttttgcgt agattgttat aacttggatt gtctttgtag
     1861 aactggagta attgcttttt gactatctgt atttgtgctg tcaggtttga aatagttgtt
     1921 tgaatatggg atttgatata gtcgggtgct tcgtgttgtt tagctttttc tgttgcgcgt
     1981 tgctgtttca gatagtctaa atatcgggcg atttcctgta attgcttctg ttcttttgtc
     2041 ggcggtttcc atgcttttaa tttgtgcttt cggtcttggc aatattgggc tatcaacttt
     2101 gcgtcttgtg tatctgtttt tgatcgttgt agttctgcta tcgcatatcc ttttatcttt
     2161 cgtggattct ctacggtaat tgtatatctt gaataaagat attcggctaa tgcttcgtaa
     2221 tatgtgcctg ttgcttcgca cacgcaatgg agcttatcgt ttactttatg actttgtagc
     2281 caatttatta attgttcaaa tcctcctttg ttgttctgaa acttcttttg ataattttga
     2341 ccgtctacaa tcaaacagca atctattgtg agctttgaaa cgtctattcc taagtacatg
     2401 gtttaacctt attaattcgg gctttttgcc ctagatagtg ttcaaactta agatgtacga
     2461 aagcccacgc ttctatcttt cttacaagct gtacgctttg gccgtacttt cgaagtcgtg
     2521 ggctttactt ggtgtttcgt caaacgccaa gccctcaatg ggctgattta ctcattcagg
     2581 gcttgaagct tatcgccttt gccgtatctg attttattgg ggtcgcaggt tttggtaaag
     2641 gtttctgttg cgacccgaat gtctgatttt ttttgggcgt atctcagtct ggaatcactc
     2701 cgttcggggg tttccggtat tgaaaaacag ttcataaaaa aggaaaaggg ggtattcata
     2761 aagattgggt aaaaagcgcg cccaatcttt acaaagcttc ccccttttcc ttttttctgc
     2821 cctattttcc tgcacctaca acccccgaac gaagcgattc cagactgaga cactttaaaa
     2881 aaaacagcca ttctagcaat taaccccctt cactcagctc aagccatcct gaggggtagg
     2941 gattgaactt tctgctttac gactccgccg ccaattcctt caaacggttt tccgcgctct
     3001 tcagttgtcg tacatgagat tttgctacgg ctttccgccc attacgagaa cttgcggctt
     3061 gtccgctttc gcggactgtt ccacctgttc cgtcctttgc tgttcgtcct tataaggatt
     3121 gaaaggcaac ccgtttttca catattcttt acacattatc tttgttattt ctttcaaggg
     3181 tgttccttga tttgaatagc atgtgcaatc tgattttccg ccgtctatac atccggcgat
     3241 ttgctcaaag gtttttactt gtcggactgt gttataaata ggcttgcttt cgggcttttc
     3301 gggtaaagtc ggcacaaagt cttcaggttt caaattgtct gaatttttta aaggcatttc
     3361 ctctgatgat gcaggctgct ccgtcatcgt ctgcacaacg ctttcttttt gcgcttcctg
     3421 ctcaatccgg ctgtctgtgg ctttgctgta aactttaaaa atgccgtaac ttttccagcc
     3481 tacaaaccct acaatcgcaa tcaacgccca aaccgcccaa ggtacttttt tcttgaactt
     3541 ttggtgctgg cttgctgatt tatagtattt aaaggcttct ttcggcggtt tccaacttgc
     3601 gacttctacg ccgcttacgc ctgcgggatt gtccaacgag gttacgcatt tataccaata
     3661 atactgtttc attccgattg ccttgcgttc aaggtgtaca tgctttgaaa caaggttgcg
     3721 gacgaatata tcaagttggc tcgggtgctg cgtcatcaaa ataacggtat gcccgtgatg
     3781 gcggagttct gtcagttcct gaatataagg cggaacggga cggcctgccg cgcgtaccgg
     3841 ataagtgtag tgcgcttcgt caacaatcag cactgcgcct tccggtatga catcacgaag
     3901 cggggcggac atgatttgct cttccgtcag ttcgtgggct ttaaactgcc gtttatccaa
     3961 tccgtcgata tggcagaaat aaagcggtct gtctacctct gtgccgtctt ccaatttcat
     4021 tttgaacaat ccgtcttcgt tgttcaaaat catagagacg acgcgggagg ttttgcctgt
     4081 ccccatgttt cctgtaaaca gataaatcat gcttctacct catcctggaa agacaaacgt
     4141 cagttttttg aatgcgtgca taccaatgaa gaacgagaat gcgccgaaca ggtagcccaa
     4201 cccctgaccg aatcccgaaa ttaaaagaag gttcagtatg tcggaaggca tggaattgat
     4261 cgcatttgac gtgtagtctt tgaacttttc cagcgcgatg agatacccgg cataggttac
     4321 aaatgtcaga cctgttgcaa ggattattct gacaatcagc attttcagaa gtatgcctaa
     4381 aagtggaatc agaccggcaa gtaatggcat ttatttcccc ttcaacgaac cgaaaacgac
     4441 aaaagccgac ataatgataa aggcgagcag tacggcaaaa cggatttttt cggcaaacac
     4501 gcataacggc tcatagcttg cctgatattg cctaccgaaa acatgaaagg ttttcggctg
     4561 cggacatacg ccgttagacg gtaaaaagtt atgtgaagac catgttttat cgtctataac
     4621 ctgcggtatg cttatatcgt gaaacatgcc gtctgaaggt ttgcccatct cctgacaggc
     4681 taggatttcc ggaaaataat cgcacaaaag cccgccgtct tcgccttctt tcctttcttt
     4741 gcgatgcct
//
LOCUS       DAXHMX010000146         4738 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHMX010000146 DAXHMX010000000
VERSION     DAXHMX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.146"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(10..456)
                     /locus_tag="ACFM4S_001831"
     CDS             complement(10..456)
                     /locus_tag="ACFM4S_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051460.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNI"
     gene            complement(1021..1224)
                     /locus_tag="ACFM4S_001832"
     CDS             complement(1021..1224)
                     /locus_tag="ACFM4S_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051461.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
     gene            complement(1787..2722)
                     /locus_tag="ACFM4S_001833"
     CDS             complement(1787..2722)
                     /locus_tag="ACFM4S_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC6051462.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGILRSVNSTDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(2725..2898)
                     /locus_tag="ACFM4S_001834"
     CDS             complement(2725..2898)
                     /locus_tag="ACFM4S_001834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051463.1"
                     /translation="MIKQVANNLPDGSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVI
                     LSVKVPSKTNIRR"
     gene            2897..3165
                     /locus_tag="ACFM4S_001835"
                     /pseudo
     CDS             2897..3165
                     /locus_tag="ACFM4S_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244123.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(3233..3667)
                     /locus_tag="ACFM4S_001836"
     CDS             complement(3233..3667)
                     /locus_tag="ACFM4S_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051464.1"
                     /translation="MNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSFLGV
                     IDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSLFNS
                     SERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
     gene            complement(3847..4329)
                     /locus_tag="ACFM4S_001837"
     CDS             complement(3847..4329)
                     /locus_tag="ACFM4S_001837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051465.1"
                     /translation="MSIKYIAMPAAWKYMPTDVSRVAKEVFGYLYNSQGASTRFDKYF
                     NTIDNRADFFAASNLYEQNLGSKARWFGGADFVSRAAITGLGADGEASYITFAAGKVV
                     GNPPIYEWRAASGNALIVNGFYNFRDLFNKKTNPREWDIQQLKSEQKLLQPIQPKIFE
                     "
     gene            complement(4367..>4738)
                     /locus_tag="ACFM4S_001838"
     CDS             complement(4367..>4738)
                     /locus_tag="ACFM4S_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6051466.1"
                     /translation="AAANKGKCQDGAIGAAVGEILGEALLDGRDPGSLNVKDRAKIIA
                     KAKLAAGTVAALSKGDVNAAANAAAVAVESNALSKERMDKLTKCLSGKTCSTTMEKVN
                     AIKKDEQFSKVIDTEIQKSLF"
BASE COUNT         1461 a         1015 c          786 g         1476 t
ORIGIN      
        1 gtctagagtc tatatattac atttattttt agggtctgct agccaatttc ttgttccctt
       61 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
      121 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
      181 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
      241 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
      301 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
      361 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
      421 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
      481 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
      541 tttttacatt acttttttct ccccccaaag gaatgcattt taaaatcatg cttttcaggt
      601 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
      661 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
      721 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
      781 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
      841 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa ttagaaaatc
      901 aaaaaaaacc attaattaat ttttcttctt taaatatttt atatgatatt ataaaattaa
      961 ataacattgc gaaaacatat gaaatcatca aaataccaaa tatatagtgg gaatgcatta
     1021 ttatttagct ccttcttttg gctctgacaa attgacagtc cattgatatc caatgcttgc
     1081 atttgcaatt gctgacgcac ctgcccctgt tttccctgtt agtatcaatg gttcttttct
     1141 tgcaagatgt gcacctgcac aaccacctaa tactaaacca ccacctattg taaatccttc
     1201 ctgttgtatc tctaatgata acattttctt cttgtgccgc ccttaataca gattgtggaa
     1261 ttggattgac ctgagcagga acttctaaaa taagttttcc ttcaagctgt cttcctctta
     1321 gcagattagg tgtacttgga acattggaaa tgacaaagtg agtacgcaaa ccacctgaag
     1381 gcgatgtgtt tgaacctgtt gaaagcggcc aacaggctaa atgcgcccgc tgccgcctaa
     1441 aaggcagccc ggatgcctga ttatcgggtg tccgaggagg attaaggggg tatttgggta
     1501 aaattaggag gtatttgata cgaaaacagc cgaaatcctg tgtttgggtt tcggatgtcg
     1561 gggaagggct tttttgcaaa ggtctcaact catgttattg caaagatctc atgccttgtt
     1621 ttgccagcag gttgagttct tctccggcaa tcagcgtggt aaagccccgc cacaacacag
     1681 atctatgtac tatggaaggg cttcaattta tagctttcga aaatctttca gctttaagac
     1741 ggcctaaagt tgtaatctat aaaacaggag ctcagagttt tacggctcac tctcgcttcg
     1801 cgttaccaaa taccagctta tcggttttga acgtatagta atctatccaa tcctgcagat
     1861 attcatacgg ataagacaag tcgtactgtt tcataaaatc gaagatgtct tgatattcgt
     1921 ctgccggcag cggatcgtaa acaatcaaat ccctcggtgc gatttcttga tctatgccca
     1981 aatcgaaacc gtgcatggat gcgattttgg cgtaggtaac gatttgcggc tgcaggtaga
     2041 aatcgaaata ggacaatgtt tctttggcag ccatacgcgc ggttttttta tcgaaaagcg
     2101 gctctaaggc ggccttcatc gcctcggcat cctgttcggc gaaagcgagg aagaagcggt
     2161 aatcgtacaa ccgcttttgc agccatttgc taggggtggg atgcgccaag actttctcgc
     2221 tacgttgttt caaccgatca agctgcttac cctccaccat cagcagagta ttgtaaatca
     2281 tatgacggtt gaggtcgtag cggtttacga aggcttctgt atcgttggcg atgttgtcga
     2341 tattgcgcac cagaaactca cgcagctgcg ggctgtcgct catcagcatc agaaacatcg
     2401 gatcttgaat gttgagcatg tcacagggaa agaagaaggg ttcagggtct gtagaattta
     2461 cactccgcaa aatacccagc tttcctgcaa cataagcata ttgcttgaat ttattaagat
     2521 catgttcaaa tagataggca tgggaagccg cagcttcagt gtacattaaa atatgtctca
     2581 ttgctgcaag tggaacgcct tttctttcat ctacataatc tatagcttca gggcaaccgc
     2641 tattgaaatt agcagtattg cctatgatta cattagtaat atgctcatac catttttggg
     2701 tggtcatcat attgtgcccc attgttatct ccttatattg gttttagaag gaactttgac
     2761 agaaagaata acggccttac ctgtttgacg atcaacgcct gctattgccg tttttaattt
     2821 gccttcccgc tccgctttga agacagcagc tttagcagga ctaccatcag gtaagttatt
     2881 tgcaacttgt ttaatcaaag aattctaccg acacatccaa atggccaaaa cattgaaggc
     2941 ggaaacctat ttttaccgcc cttaccattc ttgggagaaa gggctgaatg aaaacaccaa
     3001 tggactcatc cgacaatatt ccccaaacaa accgttttcc gaaacatcag cgatcgggag
     3061 atacgtaggg ttcaagatga gttgaaccac cgaccgagaa aaacacttgg ctacgaaacg
     3121 ccaagtgttt tattcttgaa tttgttccaa ccatcgatac cctagtgttg tacttgaaat
     3181 ccgaatctaa ggtcatctga aattaaattt agttttcaga caatcttttt cttcaattgg
     3241 aacgtggagt tacattttca cctaaactat gcacgctaga tttatagata aaccattcag
     3301 acagtccaat aaacattatg gttgggatta ccaaatacca aaaactaccg actctttcag
     3361 atgaattaaa taaagaaaag tacccatatg tataaaaaac aatgaaggtt aatatgacag
     3421 atacagcaag ttgattttga aatacccatc tgaataaagt tgataatatc actggaatta
     3481 gaaaccataa cataactata aacccacccc ctaccatccc aaatataaaa tctattaccc
     3541 ctaaaaaaga tagtttaaaa tcattacccc ataaacctga aaaaattaaa aggccaaaaa
     3601 atactccacc tattaagaaa atagatttaa tattttgaat aagataaaat aaataatttt
     3661 tattcatggc ttgcatccct gtttctcaga atatcccata agcttacaac catatttaat
     3721 tctagatttg taatctaaca tattaattcc atcctctatt ttttcctctc atctaaagga
     3781 tttggaatta ttgaaaatat cttatttgat gttacccctt taatcaatga caaataatcc
     3841 ttctcattac tcaaatattt ttggttgaat aggctgtaat aacttttgct cgcttttcaa
     3901 ctgttgaata tcccactccc taggattagt ttttttattg aacaagtctc taaaattata
     3961 aaatccattt actatcagtg cattgcctga cgcagccctc cattcataaa ttggaggatt
     4021 accaacaact ttacctgccg caaaagttat ataagaagct tctccgtctg cccctaaccc
     4081 agttatagca gcacgcgata caaaatcagc tccaccaaac catcgtgctt ttgaacccaa
     4141 attttgctca taaagattgc tggcagcaaa gaaatctgca cgattgtcaa tggtgttgaa
     4201 atacttgtca aatcttgtag atgccccttg tgagttatat aaatagccaa aaacttcttt
     4261 ggcaacccgt gatacatccg taggcatata cttccacgca gcaggcatgg caatatattt
     4321 aatagacata ttaatgccgt ttctgcaacc atcgcccaat gggttcctag aacagacttt
     4381 tttgaatttc cgtatcaatt actttgctaa attgttcatc ctttttgatg gcatttactt
     4441 tttccatcgt agtagaacaa gttttaccgg aaaggcattt tgtcaattta tccattcttt
     4501 ccttgctaag cgcattactc tctaccgcca cagcagccgc attcgccgca gcattcacat
     4561 cccccttgct caacgccgca accgtccctg ctgccagctt cgccttagca atgatttttg
     4621 ccctgtcctt cacattcagg ctgccagggt ctctgccgtc cagtagggct tcgccgagga
     4681 tttcaccgac cgccgctcct atcgcgccgt cctgacactt gcccttattc gccgccgc
//
LOCUS       DAXHMX010000147         4695 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHMX010000147 DAXHMX010000000
VERSION     DAXHMX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.147"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..819
                     /locus_tag="ACFM4S_001839"
     CDS             <1..819
                     /locus_tag="ACFM4S_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6051467.1"
                     /translation="ATANKGKCQDGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVK
                     KITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNG
                     KINVRDLKQTLKDEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQK
                     VKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQ
                     GLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            828..1205
                     /locus_tag="ACFM4S_001840"
     CDS             828..1205
                     /locus_tag="ACFM4S_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051468.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            1483..1857
                     /locus_tag="ACFM4S_001841"
     CDS             1483..1857
                     /locus_tag="ACFM4S_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051469.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            1943..3697
                     /locus_tag="ACFM4S_001842"
     CDS             1943..3697
                     /locus_tag="ACFM4S_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6051470.1"
                     /translation="MPSEKLTIFQTAFVMQVNIQIPYILPRCVRAEDTPYACYLKQLQ
                     VTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTAGAGAGAALGLNGAAAAA
                     TDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLMVAVATAGVADKIGASALN
                     NVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIK
                     QLDQHYITHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALVKNTDFSRMSATE
                     IEKAKAKITAYSKLVAGTASAVVGGDVNTAANAATVAVENNTLYPRCVGAKCDEFQKE
                     QQKWIRENPEEYREVLLLQTGFIPIIGDIQSFVQAQTAADHLFALLGVVPGIGESIQA
                     YKVAKAAKNLQGMKKALDKAATVATAQGYVSKTKIKIGQTELRVTAATDKQLLKAIGE
                     GRDTTGKMTEQLFDSLAKQNGFRVLSGGKYGGNNGFDHVWQAADGSVVLIVESKQIRN
                     GTVQLNPNGAGGYTQMSREWIKQVVKSLPDGSPAKAVVLKANQNGKLKTAIAGVDRQT
                     GKAVILSVKVPSKTNIRR"
     gene            3700..4635
                     /locus_tag="ACFM4S_001843"
     CDS             3700..4635
                     /locus_tag="ACFM4S_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC6051471.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDEKKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDHEIAPRDLTVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT         1492 a          890 c         1113 g         1200 t
ORIGIN      
        1 gcgacggcga ataagggtaa gtgtcaggac ggtgcgattg gcgcagccgt gggtgagatt
       61 gttggggaag ctttggttaa aaataccgat tttagcgata tgaccccgga acaattagat
      121 ctggaagtta agaaaattac cgcctatgcc aaacttgcgg caggtacagt tgcaggcgta
      181 acgggaggag atgtcaatac tgctgcacaa accgcacaaa acgcggtaga aaataatgcg
      241 gttaaagctg ttgtaactgc tgcaaaagtg gtttataagg tagccagaaa aggattaaaa
      301 aacgggaaaa tcaacgttag agatttaaaa cagacgttga aagacgaagg ttataattta
      361 gccgacaacc tgaccacctt attcgacgaa acattggatt ggaacgatgc caaagccgtt
      421 attgatattg tcgtcggaac agagctgaat cgcgctaata aaggggaagc ggcacaaaag
      481 gtcaaggaag ttttagaaaa aaatcgtcct tatatcccta ataaaggtgc tgtaccgaat
      541 atgagtacat acatgaaaaa taatcctttt ggaaaacagc tggctcaaat ttcagaaaag
      601 acaacgcttc cgacgcagca agggcagtct gtcttcttgg taaaaagaaa ccaagggtta
      661 ttaaaaaccg gtgataggtt ttatttagat ggccaacata aaaatcattt agaggttttt
      721 gataaaaatg ggaactttaa gtttgttcta aatatggatg gttcgcttaa ccaaatgaaa
      781 actggggcag caaaaggtcg taaattaaac ttaaaatagg aaattttatg gaaacattga
      841 atgatataaa aaaaatcttg attaatgtgg ggctttatca agggtttgat ttgacagatc
      901 caaaagtatc agaagaagtt aatcatgaaa cagctaatat gaaatggatt aaagattata
      961 cttcagacgg gaattgggat aatgaattta aggaggattt aaaaaacttt ttagattata
     1021 tggaagtatg ccaattagcc ctaaacgata aaaatttcaa aattgccagt aattctttat
     1081 ttatggctat gatttacgca ggtaatctat ctcttatatt tgattcaata aaaactgata
     1141 tatcaacatt attgagtgct gagtataaaa agaatagttt ttcatggcca tctcttgatg
     1201 aatagaaagc aagttgtagc ctgcatgaaa tctaaaaccc atgcataagg tgtgggcttc
     1261 agtatacgcg ttccatgatt tacggccata tgccgtctga aaagctcaat tttttcagac
     1321 ggcatttgtt atgaaagtaa atatttagat tccctgtata ctgtttagac tcgtgtgtgc
     1381 tgagtaagct gtagtctgca tgaaacctaa aactcgctca aaattaagct aagacattag
     1441 cagggcaagg gcgaaaattg aatcttaaat aaggtgattc agatgaaaaa ttttaatgta
     1501 gtaaaagaaa gtttaagaga gttaggaatt aaacaaggat ttgatcttta tgagaaagcc
     1561 acaactgaaa aattgaatag tgaagatcct cttgacttac aatggctttc taactattca
     1621 tctgattgga atgatgaatt agaagaagac tttgattctt tttttcagca tatgaaggaa
     1681 tatcaatatg ctattgacaa tgaagacatt aaatctgcat gtagttcact atgtgaagct
     1741 atgctctatg ttggtaatat taaaaatttt tttgagtttc tcaaaagcga tatgattaga
     1801 ctgttgagag gtgaaagtaa aacaacagac tttcaatggc cgcaatttga tgaatagcag
     1861 caagctgtag cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctt cagcacgcac
     1921 gcgttccatg atttacggct caatgccgtc tgaaaagctc acaatttttc agacggcatt
     1981 tgttatgcaa gtaaatattc agattcccta tatactgccc agatgcgtgc gtgctgaaga
     2041 caccccctac gcttgctatt tgaaacagct ccaagtcacc aaagacgtca actggaacca
     2101 ggtacaactg gcgtacgaca aatgggacta taaacaggaa ggcttaaccg gagccggagc
     2161 agcgattatt gcgctggctg ttaccgtggt tactgcgggc gcgggagccg gagccgcact
     2221 gggcttaaac ggcgcggccg cagcggcaac cgatgccgca ttcgcctcgc tggccagcca
     2281 ggcttccgta tcgctcatca acaacaaagg caatatcggt aacaccctga aagagctggg
     2341 cagaagcagc acggtgaaaa atctgatggt tgccgtcgct accgcaggcg tagccgacaa
     2401 aatcggtgct tcggcactga acaatgtcag cgataagcag tggatcaaca acctgaccgt
     2461 caacctggcc aatgcgggca gtgccgcact gattaatacc gctgtcaacg gcggcagcct
     2521 gaaagacaat ctggaagcga atatccttgc ggctttggtg aatactgcgc atggagaagc
     2581 agccagtaaa atcaaacagt tggatcagca ctacattacc cacaagattg cccatgccat
     2641 agcgggctgt gcggcagcgg cggcgaataa gggcaagtgt caagatggcg cgataggcgc
     2701 tgcagtcggt gagattgttg gtgaggcttt ggttaagaat actgatttca gtcgtatgag
     2761 tgcgaccgaa atcgaaaaag ctaaagcgaa gattactgcc tattcaaaac tggttgccgg
     2821 cactgcgtct gccgttgtag gcggggatgt gaatacagcg gcgaatgcgg ctactgtggc
     2881 ggtggagaat aatactttgt atcctagatg cgttggtgca aagtgtgatg aatttcaaaa
     2941 ggaacaacaa aaatggatac gtgaaaatcc tgaagaatat cgagaagttt tgcttcttca
     3001 gacaggattt attccaatta tcggtgatat acagagtttt gtacaagcac agaccgctgc
     3061 cgatcacctg tttgctttgc tgggtgtggt tccgggtatc ggtgaatcga tacaggccta
     3121 taaagtagcg aaagcggcaa aaaatttaca aggcatgaaa aaagccttgg acaaggcagc
     3181 aaccgttgcc actgcacagg gctatgtcag taaaaccaaa atcaaaatcg gtcaaactga
     3241 attaagggtt actgcagcaa ctgacaaaca attgctgaaa gctattggcg aaggaaggga
     3301 cacgacaggt aaaatgaccg agcagttatt tgactcttta gctaaacaaa atggcttcag
     3361 agtgctttcg ggcggcaaat acggcggaaa taacggtttt gatcatgtat ggcaggctgc
     3421 cgatggtagt gttgttttga ttgtagaaag taagcagatt aggaacggta cggtacagct
     3481 gaatccgaat ggtgcgggtg gatatacgca gatgagtcgt gaatggatta aacaagttgt
     3541 aaaaagttta cctgatggta gtcctgctaa ggcagttgtc ttaaaagcaa atcagaacgg
     3601 caaattaaaa acggcaatag caggcgttga tcgtcaaaca ggtaaggccg ttattctttc
     3661 tgtcaaagtt ccttctaaaa ccaatataag gagataacaa tggggcacaa tatgatgacc
     3721 acccaaaaat ggtatgaaca tattactaat gtaatcatag gcaatactgc taatttcaat
     3781 agcggttgcc ccgaatctat agattatgta gatgaaaaaa aaggcgtgcc gcttgcagcg
     3841 atgaaataca ttttaatgta cactgaagct gcggcttccc atgcctatct atttgaacat
     3901 gatcttaaga aattcaagca atatgcttat gttgcaggaa agttgggtat tttgcagagt
     3961 gtagatgatg aagaccccga acccttcttc tttccctgcg acatgctcaa cattcaagat
     4021 ccgatgtttc tgatgctgat gagcgacagc ccgcagctgc gcgagttttt ggtgcgcaat
     4081 atcgacaaca tcgccaacga tacagaagcc ttcgtaaacc gatacgacct caaccgtcat
     4141 atgatttaca atactctgct gatggtggag ggtaagcagc ttgatcggtt gaaacaacgt
     4201 agcgagaaag tcttggcgca tcccacccct agcaaatggc tgcaaaagcg gttgtacgat
     4261 taccgcttct tcctcgcttt cgccgaacag gatgccgagg cgatgaaggc cgccttagag
     4321 ccgctttttg ataaaaaaac cgcgcgtatg gctgccaaag aaacattgtc ctatttcgat
     4381 ttctacctgc agccgcaaat cgttacctac gccaaaatcg catccatgca cggtttcgat
     4441 ttgggcatag accacgaaat cgcgccgagg gatttgactg tttacgatcc gctgccggca
     4501 gacgaatatc aagacatctt cgattttatg aaacagtatg acttgtctta cccgtatgaa
     4561 tatctgcagg attggataga ttactatacg ttcaaaaccg ataagctggt atttggtaac
     4621 gcgaagcgag agtgagccgt aaaactctga gctcctgttt tatagattac aactttaggc
     4681 cgtcttaaag ctgaa
//
LOCUS       DAXHMX010000148         4691 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHMX010000148 DAXHMX010000000
VERSION     DAXHMX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.148"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(420..956)
                     /locus_tag="ACFM4S_001844"
     CDS             complement(420..956)
                     /locus_tag="ACFM4S_001844"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6051472.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(990..1346)
                     /gene="folB"
                     /locus_tag="ACFM4S_001845"
     CDS             complement(990..1346)
                     /gene="folB"
                     /locus_tag="ACFM4S_001845"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6051473.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1402..2004
                     /gene="plsY"
                     /locus_tag="ACFM4S_001846"
     CDS             1402..2004
                     /gene="plsY"
                     /locus_tag="ACFM4S_001846"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6051474.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALVWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNIVKLLEGRESKIGGSR"
     gene            2081..2926
                     /locus_tag="ACFM4S_001847"
     CDS             2081..2926
                     /locus_tag="ACFM4S_001847"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6051475.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRHVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3172..4146
                     /locus_tag="ACFM4S_001848"
     CDS             3172..4146
                     /locus_tag="ACFM4S_001848"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6051476.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(4289..4507)
                     /locus_tag="ACFM4S_001849"
     CDS             complement(4289..4507)
                     /locus_tag="ACFM4S_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6051477.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            complement(4483..>4691)
                     /locus_tag="ACFM4S_001850"
     CDS             complement(4483..>4691)
                     /locus_tag="ACFM4S_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051478.1"
                     /translation="GKAENLCVKRDIVNVFINRTAVLKENFAVSDKTLDKNIIFVIIN
                     VKIQFDFLTVNQERKAICSQNTVI"
BASE COUNT         1207 a         1403 c          985 g         1096 t
ORIGIN      
        1 cgttctcaaa ttcaaattgg gcttttaggt ctataacaaa gaaaaagcct tggtaggata
       61 aacctaccaa ggctttttct taaaaaaacg ctctattacc gtgcctaaaa atgcaaaaaa
      121 gtaaccgtca taaccctttt gccaaaatca atatcatcat ccgatgaatg aattgacggt
      181 atataagaaa gaattaataa gctcataaac cggtaagaaa aatcagtaac aaagatattc
      241 attattaacc attttaaaaa ttatcatgtt cgtttgggta attattatac ccttttccct
      301 cttattacta aagcacgaat gactttaaag gacgcgccca gagacaagcc gaaaaggcat
      361 atctcatccg ccaaacaaaa tgcccaccga cttactccgg caggcagttc aacaccctgt
      421 caatccttca tcagccagta ttgcaaaccg attaatgtct tgccgtcttt aatttcatcg
      481 tttgctaatg cctgacggac ttcttctttc gacatcaata cggtttccgt aatctcgtct
      541 tcgtcattgg caagcgtact gcccaaacgc acgccttccg cttcgaacag atacattttt
      601 tcgttgcaaa aaccgaccgc cgtataaaaa ctgtaaagca ggcgcacgct gtcggcaaca
      661 taaggcgttt cttcagccag ttctctcagc gcacacgctg ccatatcctc gcccgccaca
      721 tccagcttgc ccgcaggaag ttccaatgtc gcctgatttg ccgcataccg ccactgccgt
      781 accaaaacga ccttttcatc atccgttacc gccaacacgc acgccgctcc cggatgacgg
      841 atgacgatac gctgcccttc attgccgttg ggcaacctga ccttatccct gctgatgctg
      901 acaaaaccgc cctcgtaaat ggtttcgccg cctaatttta cttccctcaa atccacttca
      961 tatctccttt ccgtttccta ttctgccgtt caatcttcac gcttgccgcg ctcgatttcc
     1021 acgccaacct cgcgcacgcc ttccaaaata cccggcttga cgattttcac gcgtacccac
     1081 accgcgccga aatatcccaa aaccaaatcg gcaatatatt ccgccaacgc ttccaaaagc
     1141 aggaaatcct gttctttcag atgtcggcgc agtgtttcgc atacctcggc ataatgtacc
     1201 gtattggcaa tatcgtcgtc cgaacccgct ttctcgggaa cgccgatgtc caaatcgaca
     1261 atcagggtct gcaaccgttc gcgttcccag ccgtacacgc cgataagcgt atccgccttg
     1321 atgccgtgca aaaagatttt atccataatc cgccccgttt tttgctaaaa taaccgcacc
     1381 attctaagta aagtaccaca aatgttcaac ataccggctg ttgccgtttc ctatctgatc
     1441 ggctcacttt ctttcgccgt catcgtttcc aagtattacg ggatggacga cccgcgcacc
     1501 tacggatcgg gcaatcctgg ggcgaccaat gttttacgca gcggcaaaaa aaaggcggcc
     1561 gcgctgacgc tcttgggcga tgccgccaaa ggtttagttg ccgttttgct tgcacgcgtg
     1621 cttcaagaac cgctcggttt atccgacagc gcaatcgctg ccgtcgcact cgccgcgctg
     1681 gtcgggcata tgtggccggt gtttttcgga tttaagggcg gcaaaggcgt ggcaacggca
     1741 ttgggcgtgc ttctggcact ctctcccgca actgccttgg tctgcgcgtt ggtttggctt
     1801 gttatggcat tcggcttcaa ggtgtcctcc cttgccgcat taaccgccac aatcgccgcc
     1861 ccccttgccg cactgttttt tatgccgcat acttcttgga ttttcgcaac cctcgcaatc
     1921 gccatattgg tgttgctccg ccacaaaagt aatatcgtca agctgctcga aggcagagaa
     1981 agcaaaatcg gcggcagccg ctgatttccc atccatccct gtttatgccg tctgaacccc
     2041 gcttcagacg gcatccgaat atcccgccgt tttaccgact atgctcagtt acagacacgc
     2101 attccacgcc ggcaaccacg ccgatatgct caaacacttc accttgtttt tagtgctgca
     2161 atatttcaac cgcaaagaca agccctactg gtacatcgac acgcacggcg gcgcgggtgt
     2221 gtacaatctc gaaggcagcg aggcacaaaa agtcggcgaa taccggcaag gcatcgccct
     2281 acttcgacaa gcaaaaaacc tgccaaccga gttaaacggc tttatcgcgc acatacaaaa
     2341 aatcctgccc tcgcccgaac tttactgcgg ttctccgtgg ctggcgcaat cgctgacccg
     2401 cgtcggcgac aaattacgcc tgtttgagct gcaccccacc gactttgtcc atctgcaaaa
     2461 caatatgggc gaagcaggat tgggcaaacg cggacaagtg ttgcgcgaag acggttacaa
     2521 agggctgatt tccctgctgc cgcccccgcc gcgccgcgcc accgtcctca tcgacccgcc
     2581 ctatgaggaa aaacaggact accggcatgt aacggaaacg ctgaaagccg ctttaaaacg
     2641 gtttgagtcc ggctgttatc tcatttggta tccctgcctc agccgcgaag aaagccgcaa
     2701 gctgcccgaa gaattgaaaa aactcctgcc cgacaactac cttcacgccg aacttcacgt
     2761 ccacgcgccg aaagccgacg gtttcggtat gcacggcagc ggaatgttcg tcatcaatcc
     2821 gccctatctg ttggcggaac aattggcggc aaacctcccc gccctgacgc ggctgctggc
     2881 gcaagacgaa ggcgcgcgct accttttgga cagcaaaatc cgctgacccc gatgccgtct
     2941 gaaacccttc agacaacatc tcccgtcaaa taaacaaaac cggacgatat accggcagtc
     3001 ggcactttta agaataaata tcccctcatc cgcctgccgc gttccgactt gccccatgcc
     3061 gtttttttac actacaatcc cctagactat cctgcaaaac gcaccaaagg aatacgccac
     3121 aataacctgc cgcccttcag acggcacaaa cctttccgaa acgagcaaac tatggacaca
     3181 ctgacccgat tcctccccga acacctgcaa caaaaccagc tgcccgaagc actcggcggc
     3241 gttttactgt ccgttgtttc cgcctgcacc gaaatcaacg ccaaagtccg cctcggcgca
     3301 ctggccggcg tattgggtat ggcgggcacg ggcaatattc agggcgaaga ccaaaaaaaa
     3361 ctggatgtta tcgccaacaa catcatgatt gacacactca aagccaaccc tgccgttgcc
     3421 ggtttggcaa gcgaggaaga agactctttc gtaagtgccg gtgaaaacgg acgctatctc
     3481 gtcctattcg accctttaga cggatcgtcc aatattgatg tcaacatttc cgtcggtaca
     3541 attttctcca tccttgaaaa acccgaaggc gcattggcaa ccgaatcatt ccttcaaacg
     3601 ggcagacagc agcttgccgc aggctatatc ctctacgggc ctcaaaccca gctcgtattc
     3661 acattcggac acggcgtata tatgttcacg ctcaatgccg aaaacgaatt tgtgctgacc
     3721 aaagaaaaac cgaaagtacc cgaaagtacc aaagaatttg ccatcaatat gtccaaccgc
     3781 cgccactggc tgccccccgt ccaacaatac atcgacgagc tcttggcggg cgaaaccggc
     3841 acgcgcggca aaaactacaa tatgcgctgg gtagccagca tggttgccga aatccaccgc
     3901 atcctgatgc gcggcggcgt gttcatgtat ccgcaagaca aacgcgaccc gtccaaaccc
     3961 ggcaaactgc gcctgatgta cgaagccaac cctatgagcc tgatactcga acaggcgggc
     4021 gcatcggcaa gcaacgcata tcaagccatg ctcgacatac agcccgaaaa cctgcaccaa
     4081 cgcgtcgccg tcttcatggg cagcagcgaa gaagtcgcct atctcgaccg cctgcacgca
     4141 aaatagacag acagccgttc acacaagtac acggcggcgg caatccgata ccgccgccgt
     4201 tatttcaaac agcacaagcc ttctggtcaa atgacaaaaa tgccgtctga aaacatttca
     4261 gacggcattt caaaaactca aacccgaatt attttcccgc tgctttttgc aggatggcgt
     4321 acaactcgtc tttcagtttc aatttggctt ttttcagctc atcgatggtt tccgcaccgc
     4381 tggtaaccgg attgttgacc agaccggtaa ttttatcgtc cagctcgttg tgttcgtcga
     4441 acagacgggc gaagcgggaa ttttcctgtt tcaatttgga aatcaaatca cggtattctg
     4501 ggaacatatc gctttcctct cttggtttac ggttaaaaag tcaaactgaa tctttacatt
     4561 aattataaca aagataatat ttttatccag tgttttatcg gatacggcaa aattttcttt
     4621 taacaccgcc gtccggttga taaaaacgtt gacaatatca cgctttacgc ataaattttc
     4681 agcctttcct a
//
LOCUS       DAXHMX010000149         4672 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHMX010000149 DAXHMX010000000
VERSION     DAXHMX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.149"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            119..1294
                     /locus_tag="ACFM4S_001851"
     CDS             119..1294
                     /locus_tag="ACFM4S_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6051479.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGEGDDVVVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDDIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRMPEWTARRRENAAKLAESLG
                     KFKSIRLIEVAGYIEHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKKHIEAVLTEAAR"
     gene            1342..3252
                     /gene="pglD"
                     /locus_tag="ACFM4S_001852"
     CDS             1342..3252
                     /gene="pglD"
                     /locus_tag="ACFM4S_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6051480.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFISFRILSTALAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWRTVICGLAVYRPDDIAALIGRYGVEKILLAIPGATQEQ
                     RRRIISKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDPVTPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            3308..4600
                     /locus_tag="ACFM4S_001853"
     CDS             3308..4600
                     /locus_tag="ACFM4S_001853"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6051481.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSAKPK
                     IENVEHEGEAGFFKYRVNFDALENLPELKEGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAQKHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEILKAEGTLIIPGEHFFIGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
BASE COUNT         1141 a         1499 c         1149 g          883 t
ORIGIN      
        1 cacccccgta cagaccgatt ttgacaacac ctgcggcgcg cgcccgattc ctcgaaacac
       61 gcccccttca gacggcatag ggtcggaaat gccgtctgaa cccgacggac aaaccatcat
      121 gttgaacact tccctttccc cgtggccctg cttcacccaa gaagaagccg atgccgtttc
      181 caaagtcctc ttgtccaaca aagtcaatta ctggacgggc agcgaatgcc gcgaatttga
      241 aaaagaattt gccgcctttg ccggcacgcg gtacgccgtc gccctttcca acggcacgct
      301 ggcactcgat gccgcgctca aagcaatcgg cataggcgag ggcgatgatg tggttgttac
      361 ctcgcgcacc ttcctcgcct ccgcgtcctg catcgtgaac gcgggcgcaa accccgtgtt
      421 tgccgacgtg gatttgaaca gccaaaacat cagcgcggaa accgtcaagg ctgtgctgac
      481 cccgaatacc aaagcgatta tcgtggttca ccttgccggt atgcctgccg aaatggacga
      541 catcatggct ttggcaaaag aacatgattt gtgggtaatt gaagactgcg cccaagcgca
      601 cggcgcaaaa tacaaaggca aatccgtcgg ctctatcgga cacgtcggcg cgtggtcgtt
      661 ctgccaagac aaaatcatga ccaccggcgg cgaaggcggt atggttacga ccaacgacaa
      721 aaccctgtgg gaaaaaatgt ggtcgtacaa agaccacggc aaaagctacg atgccgtgta
      781 ccaccgcgaa cacgcgcccg gtttccgctg gctgcacgaa agtttcggca caaactggcg
      841 tatgatggaa atgcaggcgg tcatcgggcg catccagctc aaacgtatgc cagaatggac
      901 ggcgcgccgc cgagaaaacg ccgccaagct ggcggaaagc ctgggcaaat tcaaaagcat
      961 ccgcttgatt gaagtcgccg gctacatcga acacgcgcaa tacaagtttt acgccttcgt
     1021 caaacccgaa cacctcaaag acggctggac gcgcgaccgc atcgtcagcg aactgaacgc
     1081 gcgcaaagtc ccctgctatc aaggcagctg ctcagaagtg tatttggaaa aagccttcga
     1141 caacacgccg tggcggccca aagagcggct gaaaaacgcc gtcgagttgg gcgataccag
     1201 cctgatgttc ttggtgcacc cgacgctgac cgacggcgaa atcgcattct gcaaaaaaca
     1261 catcgaagcc gtattgaccg aagccgcacg ataacccttc agacggcata tgccgcctga
     1321 aagcacatac cgccccacga tatgactctg gaaaccctga tcgccctgcc gcgcaacatc
     1381 aagaaaatct gtttcctcat acacgatttt ctgatgattt tcattgcatt ctggttcacc
     1441 caaagcctaa aggccgacta ctcgaacgaa tggttcgacc ctgccaactg gcagtctttc
     1501 ctattgaccg cgctgttgac catcggtctg ttcgtcagaa tggggctcta ccgtgccgtt
     1561 acacgcttca tcagcttccg catcctctcc accgcgctgg caggcagcat tgtttccgcc
     1621 gtcctgttct tcctcaacac attgattttt gaagaaaggc tgcgcctcgc cctgccgatt
     1681 gtctatttct tactgctgtt tgtttccgtg accggctcgc gtatggtttt gcgcggactg
     1741 ttgtccgaac accccaaaaa acagatgatc cccgtcatca tttacggcgc gggacggtcg
     1801 ggcagacaac tgcttgaggc cgtcaaacaa atgcgcgaat attccgccgc cgcctttgtg
     1861 gacgacgacc ccaaactgtg gcgcaccgtc atctgcggcc ttgccgttta ccgccccgac
     1921 gacatcgccg ccctcatcgg acgctacggc gtggaaaaaa tcctgctcgc catccccggc
     1981 gcgactcagg aacaacgccg ccgaatcatc agcaaactgg aagcctatcc gtgcgaagtg
     2041 ttgaccattc ccggaatgaa agacctgatg gacgggaaaa tcagcatcgg cacgctcaaa
     2101 aaaatctccg tctccgacct gctcgggcgc gaccccgtca cgcccgacga ccgcctgatg
     2161 agtgccgaca tcgaaggcaa aaccgtcatg gtaaccggcg cgggcggctc catcggttcg
     2221 gaactctgcc gccagattat ccgccgccgc cccgaaaagc tgctgctgtt cgagttatcc
     2281 gaattcgccc tgtacgccat cgaaaaagaa ttgcgcgaaa cctgcatcca aaaacgcctc
     2341 gacaccgaaa tcctgccctt tctcggttcg gtgcaaaacc gcacgctgct cgaacacgtc
     2401 atgaccgcct tttccgttgc gaccgtctat cacgccgccg cctacaaaca cgtccccatg
     2461 gtcgagttca acaccgtcga aggcatacgc aacaacatct tcggcacact cgaatgcgcg
     2521 cttgccgcca cggcttcggg cgtggaaacc ttcgtcctca tctccaccga caaagccgtc
     2581 cgccccacca acacgatggg cgccagcaaa cgtatggcgg aactctgcct acaggcgctc
     2641 gccgccgaac ccggacaaaa aacccgcttc agcatggtgc gtttcggcaa tgttttaggc
     2701 tcgtccggct ccgtcgtccc gctgtttgaa aaacagattg cagaaggcgg cccgcttacc
     2761 ctgacccacc ccgacatcac acgttatttc atgaccatac ccgaagccgc ccaactcgtc
     2821 atacaggcag gcgcgatggg tactggcggc gacgtattcg tcctcgatat gggcgaatcc
     2881 gtcaaaatca tcgaccttgc ccgccaaatg attaccttaa gcggcctcaa acccaaaaca
     2941 cccgaacaac ccgacggcga catcgaaatc ctcattaccg gactgcgtcc cggcgaaaaa
     3001 ctctacgaag agctgctcat cggcgacaac gtccgcaaaa ccggccatcc gcgcattatg
     3061 acggctgacg aggcgatgct gccgtggcac gagctctctg tcctgctcga ccgcatccgt
     3121 gcggcctgcg accgttacga ccagcaggca atccgcaccc tgctcatcaa cgccccgacc
     3181 ggctttgccc cgagcgacgg tatctgcgac ctgctttggg tacgagaaac acacagaaaa
     3241 aatgccgtct gaaccttcag acggcataac gtacaaacca acctacctta cacacacgga
     3301 gtttgacatg cagttttcgg cattcggcga aaaattcacg caacacagcg gcatcctcca
     3361 actgatggac gacctcggcg acgcgctcaa aagcgacaag cccgtcaaca tgctcggcgg
     3421 cggcaacccg gcgcgcattc cggaaatcga tcgggcgttt gccgacatat tctccaaact
     3481 ggcggcagaa cacgccgtcg aaaacatcgg caactactcc aatcctcaag gagatgccgc
     3541 gctgattgac gcgctaaccg ccttcctcaa ccgcgaatac ggctggaacc tgaccgccga
     3601 caatatcgcg ctgaccaatg gttcgcaaaa cgcgtttttc tatttattca acctcttcgg
     3661 cggcaaattc aagctttcag acggcacatc cgcagaaaaa gccattttgt tgccgctcgc
     3721 gcccgaatac atcggctatg ccgacgtgca tatcgaaggg cagcacttcg tttccgccaa
     3781 acccaaaatc gaaaacgtcg aacacgaagg cgaagcaggc ttcttcaaat accgcgtgaa
     3841 ctttgacgca ctggaaaacc tgcccgagct gaaagaaggc aaaatcggcg cgatttgctg
     3901 ttcgcgcccg accaacccga ccggcaacgt gctgaccgac ggcgaaatgg cgcgtttgga
     3961 cgctttggcg caaaaacacg gcattccgct gattatcgac aacgcctacg gaatgccgtt
     4021 ccccaacatc atttacagcg acgtaacgct gaattggcat gaaaacatca tcctctgctt
     4081 cagcctttcc aaagtcggtc tgccgggcgt gcgcaccggc atcatcgtcg ccgcgcccga
     4141 agtcgtcaaa gccgtcagca gcctgaacgc gattgtgaac cttgccccca cgcgcttcgg
     4201 cgcggccatc gccgccccgc tgctggaaag cggcgagatg aaacggcttg ccgaccaagt
     4261 catccggccg ttttaccgca atcaggcgca aaccgccgtc tcgctgctca agcgcgagct
     4321 gggcgcgtac ccgatgaaaa tccacaaacc cgaaggcgcg attttcctgt ggctctggtt
     4381 tgaaaacctg cccgtttctt cgcaaaccct gtacgaaata ctcaaagccg aaggcacgct
     4441 gattattccg ggcgagcatt tcttcatcgg catcgacacg caggattacc cgcacgcggg
     4501 cgagtgcatc cgcatgagca tcgcgcagga cgctcaaacg ctggaaaaag gcattgccgc
     4561 catcggcaaa accgtccgaa aactgtacga caacgtttaa aacgcaaaaa aatgccgtct
     4621 gaaaagtttt cagacggcat gagacctttg caataacata ggttactaaa at
//
LOCUS       DAXHMX010000150         4650 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHMX010000150 DAXHMX010000000
VERSION     DAXHMX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.150"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            110..1051
                     /gene="miaA"
                     /locus_tag="ACFM4S_001854"
     CDS             110..1051
                     /gene="miaA"
                     /locus_tag="ACFM4S_001854"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6051482.1"
                     /translation="MPTPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSHAERSFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVTDPFSDGNSCTRLIEAAKRFFGA"
     gene            1055..1774
                     /gene="tadA"
                     /locus_tag="ACFM4S_001855"
     CDS             1055..1774
                     /gene="tadA"
                     /locus_tag="ACFM4S_001855"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6051483.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGAVQTFLLLKASGLT
                     LTKSTLWQLQSLLDGTPPQEMAQHDKTRLLAELKNHPPVAAFPPQGEMAHFMGLALEQ
                     AKLSALMGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1779..2267
                     /locus_tag="ACFM4S_001856"
     CDS             1779..2267
                     /locus_tag="ACFM4S_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6051484.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGMFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2333..3085
                     /gene="rlmB"
                     /locus_tag="ACFM4S_001857"
     CDS             2333..3085
                     /gene="rlmB"
                     /locus_tag="ACFM4S_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6051485.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3147..4538)
                     /locus_tag="ACFM4S_001858"
     CDS             complement(3147..4538)
                     /locus_tag="ACFM4S_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC6051486.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLIFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
BASE COUNT         1162 a         1490 c         1126 g          872 t
ORIGIN      
        1 aaggcatacg gtaaacacgc ccccttgccg tcggcgtgct tccgcaaacc aaaatataac
       61 gtcggcacgc tttccgctat aatccgtatt tttccaaacc tgaaacgtta tgccgacccc
      121 gaaagccttt gccctgctcg ggccgaccgc ctgcggcaaa accgcgctcg ccctcaaaat
      181 tgccgaaacc ttgcctgtcg aaatcatcag cctcgattcc gcgctggttt accgcgatat
      241 ggacatcggc acggcgaaac cgtctcatgc cgagcgttcc tttgtaccgc accaccttat
      301 cgacatcatt acgcctgttc agacatacag tgccgcccgt tttgtcgaag actgcacgcg
      361 tctgattggc gaaatcacgg cgcgtgggaa atatccgctg attgtcggcg gcacgatgat
      421 gtatttccgc gccctgaccc aaggtttgaa cgatttgccc gaagccgacg cctgcctgcg
      481 tgccgacttg gacgaacaaa aacaaatgta cggcttggat ttcctctacc gcaccctgca
      541 aaaagtcgac cctgaaacag catgccgtct gaaaccgaac gacagccagc gtatcggacg
      601 cgctttggaa gtttattatt taaccggcaa accgatgagt acgcatctcg gcagcccaac
      661 ctcccatacg ctccccttcg atttgcatac cgccgccctg attcccgaaa accgcgcccg
      721 cctgcatgaa aacatcgccc tgcgtttcca cctgatgctt gaacagggct ttatcggcga
      781 agtagaaaac ctgcgccgcc tctatcccag cctgaccgcc gactcccctg ccatacgctg
      841 cgtcggctac cgtcaggcat gggaatatct ggacggaaaa accgatttcc cggcatttgt
      901 cgagaaaggc attgccgcca cgcgccaact tgccaaacgc caactgactt ggttgcgcaa
      961 aacaccttta gactgcgtta ccgacccgtt ttcagacggc aattcatgca cacgcctgat
     1021 tgaggcggca aaacggtttt tcggcgcata aactatgctg accacgccgc cgctcgcccc
     1081 caaaaccgtc gccgccctac acaggctcgg tatccgtacg ctcgaagaac tgcggcaaaa
     1141 cggcgcggtt caaaccttct tgttattgaa ggcatcaggt ttgaccctca cgaaaagcac
     1201 gctgtggcag cttcagtcgc tgctggacgg cacgccgccg caagaaatgg cgcaacacga
     1261 taaaacccgc ctgcttgccg agttgaaaaa ccatccgccc gtcgccgcct ttcccccgca
     1321 aggcgaaatg gcgcatttca tgggtttggc attggaacag gcgaaactgt ccgcattaat
     1381 gggcgaaata cccgtcgggg cagtcatcgt ttcagacggc aaaatcatcg catcggcaca
     1441 caacacctgc attgccgact gcaatgtcag ccggcacgcc gaaatcaacg ccttggcaca
     1501 ggcaggcagc gaaatgcaaa actaccgcct tgacggatgc gacatctaca tcaccctcga
     1561 accctgcgcc atgtgcgcgt ccgcactgat acaggcacga atcaggcgcg tgatctacgg
     1621 tgcggcagaa cccaaaaccg gcgcggcagg aagcatcgtc aacctgtttg ccgacaaacg
     1681 cctcaatacg cacaccgcca tacggggcgg aatcctgcaa gaagaatgcc gcgccgtatt
     1741 aagccgtttt ttccaaaaca aaagaaaagg ttgaacctat gaccgccttg ctcgtcatcc
     1801 tcgccctcgc cctgatagcc gccggtacgg cgggcatcgt ttaccccgcc ctgcccggat
     1861 tggcattgat gtttgccgga acctggctgc tcgcctactc cggcggctac caaatctacg
     1921 gcgcgggcgt tttgtggacg gtcggactca tcagccttgc cggcatactg gcggactatg
     1981 tggcaggcat atgggggaca aaatataccg gagcgggcaa gctcgccgtt cgcggcgcat
     2041 tggtcggcag tatcatcggt atgtttttct cccttcccgg actaatactc ggtcccttta
     2101 tcggcgcggc ggcaggcgaa ctgatcgatc ggcgcaatat gcttcaggca ggtaaagcgg
     2161 gcttgggtac gctgttgggg cttgtcgtcg gcacggcgtt caaaatcggc tgcgccgtat
     2221 ccatcttgtt tatcctgttg gtgaaataca tcgcctacct gttttaaccc cttcagacgg
     2281 cataccggca aattgctaca atgccgtctg aacccttttc acaaagcacc atatggcaaa
     2341 ccaaagacct atctacggct tccatgccgt caacgcccga ttgtggcaaa accccaaatc
     2401 catcgtcgaa ctctatgtcc aagaaggcaa aaacgatgta cgcacgcgcg aagtgttgga
     2461 aaaggcggca aacgaaaaca tccgcgtata ttttgccgat gccgaccgcc tcaacgccat
     2521 cagcaaaggt gcgcgccatc agggcgtggt cggatttatc gatgcctcca aaaaccacgt
     2581 ccacctcgaa gacgtattgg aaaacttaag cgaaccgccg ctgctgctga tactcgacgg
     2641 catcaccgat ccgcacaacc tcggcgcgtg cctgcgtacc gccgacgcaa tgggcgtaca
     2701 tgccgtcatc gcaccgaaag acaaaagcgc ggggctgaac gccaccgtca gcaaagtcgc
     2761 cagcggcgcg gcggaaaccg tcccctacat caccgtaacc aacctcgccc gcaccctgcg
     2821 cgagctgaaa gaatacggca tttggatcat cggcaccgat atgggcggca acgccgacct
     2881 ttaccattgc gatctgcccg acagcgcggc atgggtgatg ggcaacgaag gcgacggtat
     2941 gcgccgcctg acacgcgaac attgcgacat gctggtgtcc atacccatgt tcggcacggt
     3001 cgaaagcatg aacgtctccg tgtctgccgg aatggtatta agcgaaaccc gccggcagcg
     3061 cgtattaaaa aacgaaaaag cgtaagcctc aaaaacaatg ccgtctgaaa cagggattct
     3121 gtttcagacg gcattcgttt tcaaaatcag tgttccaagt ggtcggtatc aaacttgacc
     3181 gccgcctcgg ttttatcttc gggcaagacc aaattcagca agactgccgt aatgccgccg
     3241 gcggaaatag agttttggaa caagacaggc aggtttttaa acacttccgg ctcaaacgcg
     3301 acacccaagc ccaaaccgac cgacgttgcc gcaattaccg cttcgcgcct gcggatgccg
     3361 tggctgacca aaatccgcac gcccgcaatc gcaatcaagc cgaacatcaa aaccatcgcg
     3421 ccgcccaaca ccggactcgg aatcgtcgta aacgcgcgtc cgacaacggg gaacagaccc
     3481 aacagcacca aaatcacggc aatatatttg cccacatggc gcgaagccac gccggtcatc
     3541 tgaatcacgc cgttgttttg cgcaaacgtc gtcagcggca gcgaacccaa agccgtcgca
     3601 atcaccgaca ccaagccgtc agccaacacg ccgccgcgca ggcgtttggt gtattcctcg
     3661 ccttcaatcg gctgatcgga caccattgcc gtcgccgtca aatcgccgac cgcctcaaac
     3721 acgctcaaca ggaaaatcgc gcccgccaca ataaatgcgt gccagtcgaa agcaaaaccg
     3781 tatttaaacg gtacgggcag cgtaaccagc ggcaggtttt gcagcgcgga aaaatccacc
     3841 ttgcccaaaa acagcgcgac aatgtaaccg gcaatcagac cgaccgcgat gccgctcatc
     3901 cgcaacagcg ggtttttcat gcagttgaat atcaacacaa tcagcaacac cagcgaagcc
     3961 agccccaagt tttccatcga gccgaacgtg ccgtccgcct tcgcgccgaa gccgccgccg
     4021 aaatcggtaa taccgacgtg taccaaactc aagccaatga gcatcacgac cacgccgctg
     4081 accgtcggcg taatcacttt tttcaaatac ggcaaaagcc acgccgagaa acacaccaaa
     4141 aacgcgccga caaacgatac acccaagagc gtcgaaatca tcgcatcctt agtcaaaccg
     4201 ccctccttca tccctgcgcc cagcgcaatc atcacggtaa cgaacgaaaa attcaccgac
     4261 tggatggaca gcatccccga accgaccggc ccgaagcggt tgacctgcaa ataagtgccg
     4321 acacccgacg caaccatcgc catcgacacg agatacgccg tcatctccac cggcaattcc
     4381 agcgcgccgc ccacaatcag cgcgggcgta atcatcggca caaaaatcgc caaaagatgg
     4441 gtaaccgcgc tcaagagcgc attaccgaac ggcggcctgt cttccaaacc gtacaccaaa
     4501 tcaggcgcat ccgcaccttt gcccaactga ccgctcattg tatttcctta ttttaaaggt
     4561 tgaaaaatcc aagcgcggat tgtagcgaat ctgccgcacg gcttcaattt gcatttcaga
     4621 cggcatccgt ctggtccaca cgtttaccac
//
LOCUS       DAXHMX010000151         4607 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHMX010000151 DAXHMX010000000
VERSION     DAXHMX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.151"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..128)
                     /locus_tag="ACFM4S_001859"
     CDS             complement(<1..128)
                     /locus_tag="ACFM4S_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6051487.1"
                     /translation="MKEYKVVIYQESPLSSLFFGAAKVNPVNFSAFLNKQPPEGWRV"
     gene            complement(203..1546)
                     /gene="argG"
                     /locus_tag="ACFM4S_001860"
     CDS             complement(203..1546)
                     /gene="argG"
                     /locus_tag="ACFM4S_001860"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6051488.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            2010..2810
                     /locus_tag="ACFM4S_001861"
     CDS             2010..2810
                     /locus_tag="ACFM4S_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6051489.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPAGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            2850..3836
                     /locus_tag="ACFM4S_001862"
     CDS             2850..3836
                     /locus_tag="ACFM4S_001862"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6051490.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPDIPVYLVAEDMCASG
                     CYYIAAAADKIYADSSSIVGSIGVIGSSFDATELMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            3885..4412
                     /locus_tag="ACFM4S_001863"
                     /pseudo
     CDS             3885..4412
                     /locus_tag="ACFM4S_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684692.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            4492..4607
                     /locus_tag="ACFM4S_001864"
                     /pseudo
     CDS             4492..4607
                     /locus_tag="ACFM4S_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039136685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1090 a         1178 c         1269 g         1070 t
ORIGIN      
        1 acccgccagc cttcgggggg ttgtttgttg aggaacgcgc tgaaattgac ggggttgacc
       61 tttgccgcgc cgaaaaacag gctggacaac gggctttcct gataaatgac tactttgtat
      121 tctttcatag gattcctttt aacataaggg atcatctttt cagacgaccc cttaagttgc
      181 taatgatgca aaacagcaaa ccttattgct tattgcccaa ctgcggcaat accgaacctt
      241 cgcccagcga gagcaaaccg ctttgcgaat aaatacccag tttggcgcgg gtgtcggtga
      301 tgtcgaggtt gcgcatcgtg agctgtccga tgcggtcgag cggagtgaac gcagcgtctt
      361 cgactttttc catactcagg cgttcaggtt ggtaggtcag gttgggcgat tcggtgttca
      421 gaatcgagta gtcgttgccg cgccgcagtt cgagggtaac ttcgccggta acggctttgg
      481 cgacccagcg ttgggcggtt tcgcgcaaca tcaaggcttg gctgtcgaac cagcggcctt
      541 ggtagagcaa acgtccgagg cgcaggccgt tgatgcggta ttgttcgatg gtgtcttcgt
      601 tgtggatgcc ggtcaccaag cgttcgtagg cgatgtggaa caacgccata cccggggctt
      661 cgtagatgcc gcgcgatttg gcttcgatga tgcggttttc gatttggtcg ctcataccca
      721 agccgtggcg gccgccgatg cggttggctt cgaggaagag ttcgacgggg tcggcgtatt
      781 ctttgccgtt cagtgcaacc ggcacgcctt cttcaaagcg cacgctgact tcttcggggc
      841 tgacttcaac gttttcgtcc caaaaggcaa cgcccataat gggtttgacg attttgatgc
      901 ccgagttcaa aaattccaag tctttggctt cgtgggtcgc gccgagcata ttggaatcgg
      961 tggagtaggc tttttcaacc gacattttgt agttgaagcc gttggcaatc agaaattcgc
     1021 tcatttcgtg acggccgccg agttcgtcga taaattgctg atcgagccag ggtttgtaga
     1081 ttttcagcgc gggattggtg agcaaaccgt agcggtagaa acgctcgatg tcgttgcctt
     1141 tgtaggtgct gccgtcgccc cagatgttta cgtcgtcttc tttcattgcg gaaacaagca
     1201 tcgtgccggt tacggcgcgg cccagaggcg tggtgttgaa ataggcgatg ccgccggtgg
     1261 aaacgtggaa cgcgccgcat tggatggcgg cgatgccttc gtgtgccaac tgcgcgcggc
     1321 agtcgattaa gcgggcgttt tccgcaccgt attccatcgc ttttttggga atggcgttgt
     1381 agtcgtcttc atcgggctga ccgaggttgg cagtgtaggc ataaggcagt gcgcctttga
     1441 gtttcatcca caacagcgcg gcggaggtat caagtccgcc ggagaaggcg atgccgactt
     1501 tctgaccgac ggggaggttt tgtaaaatag tatggttttg gctcataagg gtatccttcg
     1561 gacttggaaa aaggagttcg tcgtgtttcc ttttcgattg aaaccccgcc acttggacat
     1621 ctgtccttcg gggcggtaga atcagacttt atttgggagg ggcgtaatcc cttccaaatc
     1681 aggacggcac atagggcggt gctttatgtg tcgtcccgtg tgttgaaaca ttatcttata
     1741 aatataaccc attgtcagcc gttaaggctg tctgaaaaga agctttgccc gtttcgctga
     1801 aaatcttttc agacggtctt tacgccatcc tcaacctgac tccctcccct gcgggggagg
     1861 gttggggaga gggtgcagac agcagccctt aagcaaacgc tgcctgaaaa acgcaagctc
     1921 gaccctctcc ctaaccctct cccatgggag agggaacaaa tcacagcacc atcgacattt
     1981 tcagacggca tcggtaaaga aacccatcta tgaacgcgtt caacctcacc atcatcggcg
     2041 acgaaatctt acacggcagc cgccaagaca agcatttcgc ctttttcaaa tccctgctcg
     2101 aatccaaagg gctgaagctc aatcaggtgc aatatctgcc cgacgaaccc gatttgctgg
     2161 tcaggcaact gcgccgcagc ttttcagacg gcataccgac gtttgttacc ggcggcatcg
     2221 gcgccacgcc cgacgaccac acgcgccaag ccgcagcggc tgctttggat ttgcctgtcg
     2281 tccgccatcc tgaagccgcc aagtttatcg aaggcgtaac ccggaaacgc ggcgagccgc
     2341 tcgattcgcc ggaacacgcc caacgcctga aaatggcaga ttttcccgca ggcgcagagc
     2401 ttgtgcccaa cccgtttaac aacatcgccg gattttccat ccgcgagcat tatttcttcc
     2461 ccggctttcc tgtgatggcg cacccgatgg cggaatgggt tttggatact tattacgtcg
     2521 gccgcttcaa tcaaaccgaa cgcggcagcc gcagcgtgta tgtgtttgag cagcccgaat
     2581 cgcgcattac gccgcttatg gagcatatcg agcaaactta tcccggcgtg cgttcgtaca
     2641 gcctgcccag cgttggttgg acgcattcag acggcacgca ggtcaaaccg cacattgagt
     2701 tcggcatcaa agccgaagga gaagccgtca accttttgga cgcggcgtgg gcggaagtgt
     2761 tgcacagctt ggacggattg ggagcagagc tgaaaaatcg ggtaaactga aaaccgtttg
     2821 cccaaatcgg acaatattta aggaaaacca tgcaataccg aatcagaaga gaaaacgaag
     2881 cacccgaagc aaaaaatgcg ggggaaacct tatgggagcg cgacatcatg cgcgaagtgc
     2941 tgctgtccgc ctatcaggac aggcgcaggg agcggatgtg gaaaaacatc tggcgcgccg
     3001 tcagcaccct gattctggtt gccctgattg caggcatttt ccgaaaagac gaagccgcat
     3061 tgcagttggc gggcaacacg ccgcataccg cagtcgtcaa cctgtacggc gaaatcggca
     3121 acggtgtaga agaccaggtc aaaaaactca aagacggtat ggaggccgcc tacaaaaacc
     3181 cgcaggcaaa agccatcgtc atccgcgcca acagccccgg cggttcgccc gtcgtctcca
     3241 acaccgcttt tgaagaaata cgccgtctga aagcgcaaca tcccgacatt cccgtttatc
     3301 tcgtggcgga ggatatgtgt gcatccggct gctactatat tgcggcggcg gcagacaaaa
     3361 tttatgcgga ctcgtccagc atcgtcggca gcatcggcgt tatcggcagc agttttgatg
     3421 ctactgaatt aatggaaaaa atcggcgtga aacgcagggt taaaattgca ggcagcaaca
     3481 aaggcatggg cgatccgttt tcgcccgaaa cgcccgaaca gtcgaaaatc tgggaagaaa
     3541 tgctgaccgg catacacggc gagttcatca aagccgtcaa aaccggacgc ggcggccgcc
     3601 tcaaattccg acaatatccc gacgtgttca gcggtcgcgt gtacacaggc gcggatgcgc
     3661 tgaaagtcgg gctggtggac ggactcggca atatttacag cgtggcgcga gacgtggtca
     3721 aagcccccga tgtggtggat tacactccga aggacgattt cggcagaatc ttgggcagac
     3781 gcttcggcgc agaactcaaa gcaagcgtgc gcgaagccct gcaggccgtc cggtagcgtt
     3841 gtttacagta tttctaaggg aataccaaaa taaaaatata cacaaaaaca ctcacctccc
     3901 cagtatgctg ccactaagtc agccgcgtca ctatttaccg tgcacttaaa gccgtaaaag
     3961 gcaggttgtc ccgggaggtt ttgttcatcc tgataccgat cggttgttgt tgctcaaagg
     4021 acagaaggcc gccgataaac gagattacct gtttgtcgct attgacgatt tttatactct
     4081 gccattttgc cggacaaaac cgcagacagt gctgccaagt ttctgaccga acatctgtcc
     4141 gacccctgct tgtacctgat tgagtacgtt tactctgaca atgataggta atataaagag
     4201 ccgtccaaca tgctttcggt gcagcttgtt atgataatgg gattggttgg aggcttgccc
     4261 gatttgcttg tccgcagacc aacggtaagg cggagcgggt tatccgtacc ttgatggaga
     4321 tgtggcatga ggaacagtcg tttgacagtc cggaacatcg gcaaaaggag ttgtgctgct
     4381 ttattaattt aggggctgcc ctagatcact agggaaattc aaattaagtt agaattatcc
     4441 ctatgagaga aagtcgtcta agacggtata aacaaaataa actcattttg ttatcgtggc
     4501 tatgatgtat tggatgcggg cgaatttagc catttccgta tcaatcacag cacacatttt
     4561 gccgaacgac aaaaccatat taatggaact gagaactttt ggaatca
//
LOCUS       DAXHMX010000152         4506 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHMX010000152 DAXHMX010000000
VERSION     DAXHMX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.152"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(115..1053)
                     /locus_tag="ACFM4S_001865"
     CDS             complement(115..1053)
                     /locus_tag="ACFM4S_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6051491.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAETERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHEGKILQYGTPET
                     LVQTPADVQVARLMGLPNTDDDRHIPQHAVRFDQDGIACRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGEISGNDTVHIRIEDREIVRFR"
     gene            complement(1062..1937)
                     /locus_tag="ACFM4S_001866"
     CDS             complement(1062..1937)
                     /locus_tag="ACFM4S_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6051492.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2119..2195)
                     /locus_tag="ACFM4S_001867"
     tRNA            complement(2119..2195)
                     /locus_tag="ACFM4S_001867"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2159..2161),aa:Met,seq:cat)
     regulatory      2345..2444
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2535..4436
                     /gene="thiC"
                     /locus_tag="ACFM4S_001868"
     CDS             2535..4436
                     /gene="thiC"
                     /locus_tag="ACFM4S_001868"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6051493.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRG
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGGNVTQLHYARRGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1075 a         1299 c         1200 g          932 t
ORIGIN      
        1 gcggtttcag ttgtttccga taaatttttg cagccctgat aaaaaatatg gctgctttgg
       61 taaaaaaatg ccgtctgaaa ggtttttcag acggcatttg tttttaagga agcatcagcg
      121 gaagcggacg atttcccggt cttcgatacg gatgtgtacc gtatcgtttc ccgatatttc
      181 accggcgtgc cgcatatcga ggttcagcca caggatgccg tgttccggat ggaggacgga
      241 caggctgaac gattcgggca aacaggtgcg ggacaatacg cggcacgcta tgccgtcttg
      301 atcgaaacgc actgcatgtt gcggtatatg gcggtcatcg tcggtgttgg gcaaccccat
      361 cagtcgggcg acctgcacgt cggcaggcgt ttgaaccaag gtttcgggcg tgccgtattg
      421 gaggattttc ccttcgtgca tcacggcgat ttcgtccgcc gtcgtacagg cttcttcggg
      481 cgaatgggta accaaaacgg cagggatgcc gccgtttcgg atgcgttcgg cggtcatacg
      541 gcgcagcgtg ccgcgcaaat gcgtgtccaa actggaaaac gattcgtcca gcaacagtag
      601 ggaagggcgg acaaccaaag cgcgtgccag tgccaaccgt tgtttctcgc ctccggaaag
      661 tttttcaggc ttgcggtgtg cctcgttttc cagtccgact tcggcaagtg ccgccatagc
      721 aaggcgttcg gtttcggctt tcggcatttt ttgcattttc aaaccgaatg ccgcattttc
      781 cagcgcactc atatggggaa acagcgcgta atcttgaaac atcagcgaaa tacggcgttt
      841 ttcgggcggc atacgggtaa tgttttcccc gttcagctga atttcgccgc cgtccggccg
      901 gacgatgccc gcaatcatat tcagcagggt ggattttccg cagcccgacc gccccagtac
      961 ggcgagtatt ttgccgcgcc cgacagtcag gcagatgttg tcggcaaccg ttttgccgcc
     1021 gaagcgtttg cagagtccgt tcagttcaag catggcgcat cctataaacg tatgccgtcc
     1081 agccactcgg acagcggatg ggcggcatca atcagaccgt aacggcaaaa cgcgtccagc
     1141 gtaaccagtt gcgcgtcgtg catcatgttt cccgacaaca tggtggcaac caagctgccg
     1201 atgtccattt tttcaaaaca cgcaacctcg ccgtcctgat tttcaggcag gaaggtttcg
     1261 ggtaggacgg catcgaatac atacaggatt tcattgtgca caccccggct gacggggcgc
     1321 aggctgtgca gccgggatac cggacggata aacggaaaca gcgttttatc caaaccggct
     1381 tcttcgctgc tttcgcggca cacggcttca gacggcattt cgccgccgga aacaccgccg
     1441 gcggcaatat tgtcgagttt gtcgggatcg actgctttgt gcggactgcg cttgcctatc
     1501 cagaaatgcc atcggccgtc cgattcggtc agtccgttga gatggacggc gcggctgagc
     1561 agtccgaacg gacggaaagc ggcgcgttcg agcgtgaaca aggggttgcc gccgccgtcg
     1621 gtcaggtcga aacactcgtt gcgccatccg tgaagcagcc ccgccttgtt ccatgtgcgg
     1681 gcgaggtgct gcaagcggcc gcccatatct gaccagccgt ccgcattcag gaaaatgccg
     1741 tctgaagact ccgagcagcc tgcctcccag tcttttttga cgcgctccgc ccattccggc
     1801 gacagattgc ccaaaggcag acggttcaga tacagcgttt tccagcaact ttctgcaccg
     1861 taacttgctt ttgcccactc gaacagggca tcaaggtcgt gtttgctgac ggattcggta
     1921 aaacggacgg tcggcatagt cggctttctt gaagacaatg cggaagattg tagccaaact
     1981 gcctgaattt gcaaccgcaa acagacggcg ggctgccgtt ccgatattgc ggcaggaagg
     2041 aaaatccggc aaaaaacgga ggcggcagga aacaggtaaa acaaaacccc tcggcattga
     2101 accgaggggt ttgatatttg gttgcgggag caggattcga acctacgacc ttcgggttat
     2161 gagcccgacg agctaccatg ctgctccatc ccgcgtcaga agatgaaact atacggcaga
     2221 ttttttatgt tgtcaaacat tacttccgcg aaaaatcata aaattttgcc gtccggcggt
     2281 ttatgcctga tttgaaatat tattttcttt acaaaagttc atgttcgtga tttaattttg
     2341 gttaacattg aaacaggggt gctgcctgat gttcaggcgg ctgagaaata ccctttacac
     2401 ccgatcggga taatacctgc gtggggagtt ttcacggatt ctgcctttca gacagcattg
     2461 gttttcaaat gccgtctgaa aacgcgaaac gctcctgttt ctttatttta aacgagaaaa
     2521 caggagcatt ttttatgact acgccaaaaa aaaccgccaa aacttccggc aacgaagcgc
     2581 gcgaacttgc cgacttgagc gaagacatcg gcatccgctt caaatatccg aactcggaac
     2641 gcgtgtatct gcaaggcagc cgtggcgaca tccgcgtgcc tttgcgcgaa atccgccagg
     2701 acgacaccta cacggcgcaa ggcacggaag ccaacccgcc gattcccgtc tatgacacca
     2761 gcggcgtgta tggcgatccg gcggcgcaca tcgacctgaa acaaggcctg ccgcacatcc
     2821 gcaccgcatg gctgaacgaa cgcggcgata ccgaaatcct gcccaagctc tccagcgaat
     2881 acggcatcga acgcgcacac gatccgaaaa ccgcccatct gcgcttcaac caaatcaccc
     2941 gcccgcgccg cgcgaaaagc ggcggcaacg ttacccagct tcactacgcg cgccgcggca
     3001 tcatcacgcc cgaaatggag tttgtcgcca tacgcgaacg tttaaaatta gacgaattgt
     3061 cccaaaaacc ggaatacgcc aaactcttga aacagcacgc agggcaaagt ttcggcgcga
     3121 atatcccgac ccatcccgac caaatcacgc ctgaattcgt gcgccaagaa atcgccgccg
     3181 gacgcgcgat tatccccgcc aacatcaacc accccgaact cgaaccgatg attatcggcc
     3241 gcaacttccg cgtcaaaatc aacggcaatt tgggcaactc cgccgtcacc tccagcctga
     3301 ccgaagaagt cgaaaaaatg gtgtggtcgc tgcgttgggg cgcggacaca attatggact
     3361 tatccacagg cgcgcacatc cacgaaacgc gcgaatggat tatccgcaac gcgcccgtcc
     3421 ccatcggcac tgtgccgatt taccaagcgt tggaaaaaac cggcggcatc gccgaagatt
     3481 tgacttggga tttgttccgc gacaccttaa tcgaacaggc ggagcaaggc gtggactatt
     3541 tcaccataca cgcgggcgtg ttgctgcgtt atgtaccgat gaccgccaac cgcctcaccg
     3601 gcatcgtctc gcgcggcggt tcgattatgg cgaaatggtg tttggcacat catcgggaaa
     3661 atttcctcta cacgcatttc gacgaaatct gcgaaattat gaaagcgtat gacgtatcgt
     3721 tcagcctcgg cgacggcctg cgccccggct gcattgccga tgccaacgac gaatcccaat
     3781 tcgccgaact gcacaccttg ggcgaattga ccgataaagc gtggaaacat gacgtacaag
     3841 tcatgatcga aggccccggc catgtgccgc tgcaacgcgt caaagaaaat atgaccgaag
     3901 agctgcaaca ctgttttgaa gcgccgtttt acaccctcgg cccgctcgtt accgacatcg
     3961 cccccggcta cgaccacatc acctcgggca taggcgctgc caatatcggc tggtacggca
     4021 cggcgatgct ctgttacgtt acccccaaag aacatttggg actgcccgac aaagaagacg
     4081 tgcgtaccgg catcatcacc tacaaactcg ccgcccacgc cgccgacctc gccaaaggct
     4141 ggccgggcgc acagttgcgc gacaacgccc tgagcaaggc gcgtttcgag ttccgctggc
     4201 gcgaccagtt ccgcctaagc ctcgaccccg aacgcgccga aagcttccac gacgaaaccc
     4261 tgcccgccga aggcgcgaaa atcgcccact tctgttcgat gtgcggcccc aaattctgct
     4321 cgatgaaaat cacgcaggaa gtgcgcgact acgccgacaa gcaaaaagcc caacggcagg
     4381 gcatggagga aaaagcggtc gagttcgtca aaaaaggcgc gaagatttac agttgaaacg
     4441 tcaagcaaaa aatgccgtct gaaacccgga aaaaaggctt cagacggcat tctttcgctt
     4501 gtgaaa
//
LOCUS       DAXHMX010000153         4456 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHMX010000153 DAXHMX010000000
VERSION     DAXHMX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.153"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(181..333)
                     /locus_tag="ACFM4S_001869"
                     /pseudo
     CDS             complement(181..333)
                     /locus_tag="ACFM4S_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            489..>4456
                     /locus_tag="ACFM4S_001870"
     CDS             489..>4456
                     /locus_tag="ACFM4S_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFC6051494.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYTQFDVGNRGAILNNSRSNTQTQLGGWIQGNLWLARGEA
                     RVVVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTTGQPQY
                     QAGALTGFQVRSGHIAITGQGLDARDTDFTQILSHAVKIDGPVWGKDVRVSAGKNDVS
                     ADGSIQSSHSPATNINSDNSLYAIDTGALGGMYAGKITLISTDRDASVRNQGQWFASA
                     GNVAIDANGRLVNSGTMAAANAKDTDNTAEHKVNIRSQGVENSGTAVSQQGTQIHSQS
                     IQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTDTLNNQGKLSQTGSQKLHID
                     AQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASFHSSTTTPTTATGTGTATVSISN
                     ITAPTFADGTIRTHGALDNSGSIIANGQTDVSAQQGLNNAGQIDIHQLNAKGSAFDNH
                     NGTIISDAVHIQAGSLNNQNGNITTRQQLEIETDQLDNAHGKLLSAEIADLAVSGSLN
                     NQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSGRDVAIQAKSLSNNGTLAADNKLDI
                     ALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIRIKANNLDNAAQGNIQSGGA
                     TDIGTQHNLTNRGLIDGQQTKIQAGQMNNIGTGRIYGDNIAIAATRLDNQDENGTGAA
                     IAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQATGKAQRIHNAGATIEAAG
                     KMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHELLREGTQHELGWSVYNDESD
                     HLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIISGNDLTIDGKEVFNTDSQIIAG
                     GNLLVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHKGRDSTGHSEQNYTLPEEIT
                     RNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYTSNSFTPLPSSSLYIINPVN
                     KGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLGDGYYEQRLINEQIAELTGH
                     RRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQVAQLTSDIVWLVQKEVKLP
                     DGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSGSLKNSGTIAGRNALIINTD
                     TLDNIGGRIHAQKSAVTATQDINNIGGILSVEQTLLLNAGNNINSQSTAASSQNTQGS
                     ST"
BASE COUNT         1416 a          997 c          988 g         1055 t
ORIGIN      
        1 cgtccaaaat gctctttggg aatgctattg gattatagac cttacaacag aaaaaaaggt
       61 cggaaagtag aaagactgct ttttaaattc aaacggccaa gcaagtaaag acttggccaa
      121 caaatgggac aaaatccgac tgttttaaat tgccagtcaa atactggaaa acttgcgtaa
      181 atggctaaat tcactgacat caagtacatc ataactacga aaggtatccg tatacacaat
      241 gccatcagac ttaactttct ctcggataat cggcaataat gttgctgatt gcgcattagg
      301 gacaacgacg gtataaacct tgccatttcg ttcgaatggg atttttaacc gttatctagt
      361 aaagtcccta atttaataaa caaatggcag taaaatggcg gtaattgact cgccaaaatc
      421 cttgatctag aatggtcaga ctatccaatt ttttctaata actctataaa aatcaaataa
      481 ctgaaatcat gaataaaact ctctatcgtg taattttcaa ccgcaaacgt ggggctgtgg
      541 tagccgttgc tgaaactacc aagcgcgaag gtaaaagctg tgccgatagt gattcaggca
      601 gcgctcatgt gaaatctgtt ccttttggta ctactcatgc acctgtttgt cgttcaaata
      661 tcttttcttt ttctttattg ggcttttctt tatgtttggc tgtaggtacg gccaatattg
      721 cttttgctga tggcattatt gctgataaag ctgctcctaa aacccaacaa gccacgattc
      781 tgcaaacagg taacggcata ccgcaagtca atattcaaac ccctacttcg gcaggggttt
      841 ctgttaatca atatacacag tttgatgtgg gtaatcgcgg ggcgatttta aacaacagcc
      901 gcagcaacac ccaaacacag ctaggcggtt ggattcaagg caatctttgg ttggcaaggg
      961 gcgaagcacg tgtggttgta aaccaaatca acagcagcca ttcttcacaa atgaatggct
     1021 atattgaagt gggcggacga cgtgcagaag tcgttattgc caatccggca gggattgcag
     1081 tcaatggtgg tggttttatc aatgcttccc gtgctacttt gacgacaggc caaccgcaat
     1141 atcaagcagg cgcattaaca ggctttcagg ttcgttcggg tcatattgcg atcacgggac
     1201 aaggtttgga tgcacgtgat accgatttca cgcagatatt gagccatgcg gttaagattg
     1261 atgggcctgt gtggggtaaa gatgtgcgtg tgtcggcagg gaaaaatgat gtctcagctg
     1321 atggctctat ccagtcttca cactcccctg ctacgaatat aaattccgac aactcactgt
     1381 atgccatcga tacaggggca ttgggcggta tgtatgccgg taagatcacc ctgatcagca
     1441 ctgatcgtga tgcatccgtg cgcaatcagg ggcaatggtt tgcctcagcc ggcaatgtgg
     1501 cgattgatgc aaatggccgt ttagtcaata gtggcacgat ggctgccgcc aatgcgaaag
     1561 atacggataa tacagcggaa cacaaagtca atatccgcag tcagggcgtt gaaaacagcg
     1621 gtacggcggt atcgcaacaa ggcactcaaa ttcacagtca gtcgattcaa aacactggca
     1681 cattattgtc ctcaggcgaa atattgattc acaattcggg cagcctgaaa aatgaaacat
     1741 caggcaccat tgaagccgct cgtttggcta ttgataccga cacacttaat aatcaaggca
     1801 aactctctca aacaggttca caaaaactcc atattgatgc acaaggcaaa atggataacc
     1861 gtggccgcat gggtttacaa gataccgcac caaccgcgtc aaatggttca agcaatcaaa
     1921 ccggcaatag ttacaatgca tctttccatt catccactac cacaccaaca acggcaacag
     1981 gtacgggtac tgcaaccgtt tctatatcaa acataactgc gcctaccttt gctgatggga
     2041 caattcgcac tcatggtgca ctggataatt caggcagtat tattgccaat ggtcaaacag
     2101 atgttagtgc gcaacaaggt ttaaataatg caggacaaat agacattcat cagttaaatg
     2161 caaaaggttc ggcgtttgac aatcacaatg gaacaattat cagtgatgcg gtccacattc
     2221 aagccggcag cctgaataat caaaatggca acatcacaac acgccaacag ttagagattg
     2281 aaaccgatca actggataac gctcatggca agttattatc agcagaaata gcggatttag
     2341 ccgtttcagg cagcctgaac aatcaaaatg gcgaaatagc gaccaatcaa caactgatta
     2401 ttcacgatgg tcagcaatct accgctgtca ttgataatac gaatggcacg atacaatcag
     2461 gccgtgatgt tgctattcag gcaaaatcgt tatccaacaa cggcacactt gccgctgata
     2521 ataaactgga tattgcgtta caagatgatt tttatgtaga acgcaatatc gtggcgggca
     2581 atgaattgtc gctcagtaca cgaggcagcc tgaaaaattc acatactttg caagcaggaa
     2641 aacgcattcg gattaaagca aataaccttg ataatgcagc acaaggcaac attcaatccg
     2701 gcggtgcaac agacattggc acgcagcaca atttaaccaa tagaggcttg attgacggac
     2761 aacaaaccaa aatccaagcc gggcaaatga ataatatcgg tacaggtcgg atttatggcg
     2821 acaatatcgc tattgcggct acccgcttag acaatcaaga tgaaaacggt acaggtgccg
     2881 ccattgcggc acgtgaaaac ctgaatttag gcatcggaca attaaacaac cgtgaaaaca
     2941 gtctgattta cagcggtaac gatatggcgg ttggcggcgc attagatacc aatggccaag
     3001 ccacaggcaa agcccaaagg atacacaatg ccggcgcaac cattgaagcc gccggcaaaa
     3061 tgcgtttagg cgtagaaaag ctgcacaata ccaatgagca tttgaaaacg cagttggtag
     3121 aaacagggcg cgagcatatt gttgattacg aagcatttgg acgacacgaa ttattgcggg
     3181 aaggcacaca acatgaatta ggctggtctg tctataacga tgaatcagac cacttacgca
     3241 cccctgatgg agcggcgcat gaaaattggc ataaatacga ttatgaaaaa gtcacccaaa
     3301 aaacccaagt tacccaaact gcgccagcca aaatcatttc aggtaatgat ttaaccattg
     3361 atggtaaaga agtatttaat accgatagcc aaatcatcgc cggcggcaat ttgcttgtgc
     3421 aaacagaaaa agacggtttg cataacgagc aaacctttgg cgaaaagaaa gtattcagtg
     3481 aaaatggcaa attacacagc tattggcgtg agaaacataa aggacgagac tcaacgggac
     3541 atagcgaaca aaattacact ttgccggagg aaatcacacg caacatttca ctgggttcat
     3601 ttgcctatga atcgcatcgc aaagcattaa gccatcatgc gcccagccaa ggcactgagt
     3661 tgccgcaaag caacggtatt tcgctaccct atacgtccaa ttcttttacc ccattaccca
     3721 gcagcagctt atatattatc aatcctgtca ataaaggcta tcttgttgaa accgatccac
     3781 gctttgccaa ctaccgtcaa tggttgggta gtgactatat gctggacagc ctcaaactag
     3841 acccaaacaa tttacataaa cgtttgggtg atggttatta cgagcaacgt ttaatcaatg
     3901 aacagatcgc agagctgaca gggcatcgtc gtttagacgg ttatcaaaac gacgaagaac
     3961 aatttaaagc cttaatggat aatggcgcga ctgcggcacg ctccatgaat ctcagcgttg
     4021 gcattgcatt aagtgccgag caagtagcgc aactgaccag cgatattgtt tggttggtac
     4081 aaaaagaagt taaacttcct gatggcggca cacaaaccgt attggtgcca caggtttatg
     4141 tacgcgttaa gaatggcgac atagacggta aaggtgcatt gttgtcaggc agcaatacac
     4201 aaatcaatgt ttcaggcagc ctgaaaaact caggcacgat tgcagggcgc aatgcgctta
     4261 ttatcaatac cgatacgcta gacaatatcg gtgggcgtat tcatgcgcaa aaatcagcgg
     4321 ttacggccac acaagacatc aataatattg gcggcattct ttctgtcgaa cagacattat
     4381 tgctcaacgc aggcaacaac atcaacagcc aaagcaccgc cgccagcagt caaaatacac
     4441 aaggcagcag caccta
//
LOCUS       DAXHMX010000154         4390 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHMX010000154 DAXHMX010000000
VERSION     DAXHMX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.154"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            165..326
                     /locus_tag="ACFM4S_001871"
     CDS             165..326
                     /locus_tag="ACFM4S_001871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051495.1"
                     /translation="MPAPATIRQIPLFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVG
                     KAAGYRYVS"
     gene            434..958
                     /gene="nspA"
                     /locus_tag="ACFM4S_001872"
     CDS             434..958
                     /gene="nspA"
                     /locus_tag="ACFM4S_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6051496.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1094..1483
                     /locus_tag="ACFM4S_001873"
     CDS             1094..1483
                     /locus_tag="ACFM4S_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6051497.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1561..2391
                     /locus_tag="ACFM4S_001874"
     CDS             1561..2391
                     /locus_tag="ACFM4S_001874"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6051498.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2406..3863
                     /locus_tag="ACFM4S_001875"
     CDS             2406..3863
                     /locus_tag="ACFM4S_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6051499.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3904..4293)
                     /locus_tag="ACFM4S_001876"
     CDS             complement(3904..4293)
                     /locus_tag="ACFM4S_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051500.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
BASE COUNT         1036 a         1424 c          980 g          950 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaaaa ccgtatgcct gcacctgcaa
      181 caatccgaca gataccgctg ttttttccaa accgtttgca agtttcaccc atccgccgcg
      241 tgatgccgcc accaccattt aaaggcaacg cgcgggttaa cggctttgcc gtcggcaaag
      301 cagccggata ccgctacgta tcttgaagta ttgaaaatat tacgatgcaa aaaagaaaat
      361 ttaagtataa tacagcagga ttctttaacg gattcttaac aatttttcta actgaccata
      421 aaggaaccaa aatatgaaaa aagcacttgc cacactgatt gccctcgcta tcccggccgc
      481 cgcactggcg gaaggcgcat ccggctttta cgtccaagcc gatgccgcac acgcaaaagc
      541 ctcaagctct ttaggttctg ccaaaggctt cagcccgcgc atctccgcag gctaccgcat
      601 caacgacctc cgcttcgccg tcgattacac gcgctacaaa aactataaag ccccatccac
      661 cgatttcaaa ctttacagca tcggcgcgtc cgccatttat gacttcgaca cccaatcgcc
      721 cgtcaaaccg tatctcggcg cgcgcttgag cctcaaccgc gcctccgtcg acttgggcgg
      781 cagcgacagc ttcagccaaa cctccaccgg cctcggcgta ttggcgggcg taagctatgc
      841 cgttaccccg aatgtcgatt tggatgccgg ctaccgctac aactacatcg gcaaagtcaa
      901 cactgtcaaa aacgtccgtt ccggcgaact gtccgccggt gtgcgcgtca aattctgata
      961 tgcgccttat tctgcaaacc gccgagcctt cggcggtttt gttttctgcc accgcaacta
     1021 cacaagccgg cggttttgta cgataatccc gaatgctgcg gcttctgccg ccctattttt
     1081 tgaggaatcc gaaatgtcca aaaccatcat ccacaccgac aaagcccccg ccgccatcgg
     1141 cgcgtacagc caagccgtcc gagcaggcga caccgtttac atgagcggtc aaatccccct
     1201 cgatcccgcc acgatgaccg tcgtcggcaa cggcgatttc cgcgccgaag cgcgccaagt
     1261 gttccaaaac ctgcaagccg tcgccgaagc ggcaggcggc acgctggccg acatcgtcaa
     1321 actcaacgcc tacctgaccg acttgggcaa ttttgccgtc ttcaacgaag tgatggcgga
     1381 atttattgcc gagcccttcc ccgcccgcgc cgccgtcggc gttgcctccc tgccaaaagg
     1441 cgtgcaggtc gaagccgaag ccgtcctcgt tttgaacgca taattccacg ttcggactgc
     1501 atgatatttc aaaaccatgc cgtccgaacc gcaacacttt cccacttcac aacatctgca
     1561 atggcacatt acgcaatcgg cgacatacaa ggctgtttcg acgaactgac cgcgctgctc
     1621 ggcaaaatcg gtttcaacca cggcacggac accctctggc tgacgggcga catcgtcaac
     1681 cgcggcccga aatccctcga aacgctgcaa ttctgcatcc ggcacgaaaa cagcgtgcaa
     1741 atcgtcctcg gcaaccacga cctgcacctg ctcgctgtcg gctgcggcga aggcgcgccc
     1801 aaacgcagcg acacaatcga acccatactc aaacaccccg acggaaaaaa aatgctcgac
     1861 tggctgcgtg cgcaaccgct gttgatacgc gagggcagcc gcgtgatggt acacgccggc
     1921 atcctgccgc aatggcgcat cgccaaagcc gaatcgctcg ctagagaagc cgaagccgaa
     1981 ctgcgcggca aaaaatacgt caaattcttc tccaaaatgt acggcaacaa accggccgcg
     2041 tgggacgaag gtttggaagg ctatgcccgc ctgcgcttca tcgtcaacgc cttcacgcgg
     2101 atgcgcgccc tgacctttaa aaacgaactg gatttcgact acaaatccac agtgaaaaaa
     2161 atgccgcctt acctgcgccc gtggttcaaa gcccccgacc ggcaaaacct cgaccgcacc
     2221 atcatcttcg gacactggtc ctcgctgggc tacacgaatg ccgacaacgt catctcgctg
     2281 gacaccggcg cgctgtgggg cggacagctg accgccgtca acctcgaaac ggaagaaatt
     2341 acccaagtcc aagccgccgg cggcatagac tggaaaagct tcgcaaaata acggaccgcc
     2401 ccactatgca caaaatcctg cccatcgccc acgtcctctc ccgactgggt atgctgtttt
     2461 cctttatcct gctgataccc gccgccctct cctacgcctt ttcggacggc gcgtacaccg
     2521 ccttcgccac caccgcgacc gttacccttt ccggctcgtg catcgtccgg ctcgccaccc
     2581 tccggttcag gcgcgaactg cgcccgcgcg acggcttcac cctcgtcctg atgttgtggc
     2641 tggcgtttgc cgctatggcg gcgatgccga tgtacctgta tttcccgaat atgggcttta
     2701 ccgacgcatt ttttgaatcg atgtcgggac tgaccaccac cggcgcgacc gtcatccccc
     2761 acgtcgacgg gctcgccccc tccgtcaact tttggcggca tatgctcaac tggctgggcg
     2821 ggatgggcat catcgtcctt gccgtcgcca tcctgcctat gctcggcgta ggcggcacgc
     2881 agttattcaa agccgaaatc cccggcattg acaaagaaag caaaatgtcg ccgcgcattt
     2941 cccaagtggc gaaaaaactt tggttcggct acaccctgat caccatcctc gcggcagcct
     3001 gcctgcattt tgccgggatg ggctggttcg atgccgtctg tcacgcgatg gcgaccctct
     3061 cgctgggcgg gttttccacc cacgatgcca gcatagctta ttacaactcc cccctcatcg
     3121 aagcggtcat catcgttttc accattgtcg gcggcatcaa ttttgccaac catttcgccg
     3181 ccctcaacag ccgcagcctc aaaacctatt ggaaagacga agaatgccgg acgatgctgc
     3241 tgctgctctc cggcagcatc cttgccgccg ccctgtacct gtggcacacc ggctattacg
     3301 ccggttttac cgaatccctg cgctacaccg ccttcaactt cgtctccatc ggactggcaa
     3361 acgggctggc caacaccgac ttcgcacaat ggccgctcct gatttccctg tggatgtttt
     3421 tcctcgccaa catcctcgcc aactccggct cgaccggcgg cggcatcaaa accatacgcg
     3481 ccctcgtcct gttcaaattc agcctgcgcg aaatgatggt gctgctgcac cccaaagccg
     3541 tccgcaccgt caaaatcagc ggcaaggcca tacccgaccg cctcgcgctg accgttatgt
     3601 ccttcatctt catctacttt atgaccgtcg tcctcttcag cttcctgctg atggcgagcg
     3661 gtatggaatt taccaccgcc ttcaccgccg tcatcgcctg catcaccaac gccggccccg
     3721 gcctgggcga agtcgggccc gccggcaatt acgccggttt ggacgtgatg cagaaatgga
     3781 tctgcgttac cgccatgctc ttgggcaggc tggaaatctt caccgtcttc atcctcttca
     3841 cccccgccta ctggaagaaa taaaaaatgc cgtctgaaaa cttttcagac ggcatattgt
     3901 cttttaaaaa agcggaagct ctacaaaagg aaagaaatgc tgaaacctct gataaatttc
     3961 aggatatttc tctaaggctt ttaatgcttt ttcttttgaa ataggcggat catagacatc
     4021 tatccccctt aagaaggcaa tgcccgttaa tgcatttttt tgatcatttg aaatgtaacc
     4081 ataattaacg actccaatta catcataaat ttcccctata ctcttatcaa aatcaatcaa
     4141 atcaaaaata tacaatggaa ttgtatcaat aggagtatct ctgacaacta tatccaacca
     4201 taatttaaat tcagaaagat taatagcatc atagaacata cagactattg caaatcctaa
     4261 atcttcacaa ttttctttac taattttcca cataattatt tacctttatc cattgcttcc
     4321 caaccgatac atccggtatt ttagattctc ctttgccgtc aaacgctctt ttggttacct
     4381 gctttgtatc
//
LOCUS       DAXHMX010000155         4373 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHMX010000155 DAXHMX010000000
VERSION     DAXHMX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.155"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(69..2045)
                     /locus_tag="ACFM4S_001877"
     CDS             complement(69..2045)
                     /locus_tag="ACFM4S_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6051501.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLVMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFM4S_001878"
     CDS             2194..3033
                     /locus_tag="ACFM4S_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6051502.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="ACFM4S_001879"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="ACFM4S_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6051503.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1119 a         1466 c         1001 g          787 t
ORIGIN      
        1 tgaattttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggcctgtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacaaacc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgccccaaac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagcaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attaccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaatac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccaatgc tgaacatcag gaacacaatc
     1861 ccgatttcac ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ctggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaagacaaa ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacactga
     4141 ccaaagtcgt tgacggcaac atgattaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttt catttttcac cgtgatttta cccggctgtc gtcatttcta att
//
LOCUS       DAXHMX010000156         4326 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHMX010000156 DAXHMX010000000
VERSION     DAXHMX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.156"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(170..2098)
                     /gene="dnaK"
                     /locus_tag="ACFM4S_001880"
     CDS             complement(170..2098)
                     /gene="dnaK"
                     /locus_tag="ACFM4S_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6051504.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLITRSIEPCKIALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVRDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2306..2578)
                     /locus_tag="ACFM4S_001881"
     CDS             complement(2306..2578)
                     /locus_tag="ACFM4S_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051505.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2764..3450
                     /locus_tag="ACFM4S_001882"
     CDS             2764..3450
                     /locus_tag="ACFM4S_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6051506.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3582..3920
                     /gene="erpA"
                     /locus_tag="ACFM4S_001883"
     CDS             3582..3920
                     /gene="erpA"
                     /locus_tag="ACFM4S_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6051507.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3937..4083
                     /locus_tag="ACFM4S_001884"
     CDS             3937..4083
                     /locus_tag="ACFM4S_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051508.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4080..>4326
                     /locus_tag="ACFM4S_001885"
     CDS             4080..>4326
                     /locus_tag="ACFM4S_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051509.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITR"
BASE COUNT          983 a          861 c         1148 g         1334 t
ORIGIN      
        1 aatttaatcc actatataaa aaatttccag agaaccgata taacagttag aacttgggct
       61 tgggaatatc acggtagatg aatttggaac ctctgttatg ctatggtctt ttatctcaat
      121 tgaaaaaagc gcgaatcaaa cggttcgcgc ttttttcaga cggcatcaat tattttttgt
      181 cgtcttttac ttcttcaaag tcggcatcta cgacatcatc gtctttcttt gcagaagcat
      241 tggcttgttc gctttcgcct gcttgggctt cagcttgtgc ttgagcgtaa accatttccc
      301 ccagtttttg gctggctgtg cccagtgctt cggttttggc atcgatagcg gctttgtcgt
      361 cgcctttaac tgcttcttcg gcttctttca gcgcggcttc gattttttct ttctcggctg
      421 cgtcgagttt gtcgccgtag tcggccaaag attttttcac agagtgaatc agggcttcgg
      481 cttggttgcg ggaagcgacc aattcagtca gttttttatc ttcctcggca ttggcttcgg
      541 catctttcac catgcgttcg atttcttctt cgctcaaacc tgaagaacct tggatggtga
      601 tgttggctgc tttaccggtg cctttgtctt tggcggaaac gtgcaggatg ccgttggcgt
      661 cgatgtcgaa ggttacttcg atttgcggca taccgcgcgg tgcaggtgcg atgtcgccca
      721 agttgaactg acccaaagat ttgttggcag aagcgcgttc gcgttcgcct tgcagtacgt
      781 ggatggttac tgcgctttgg ttgtcttcgg cggtagagaa cacttgcgac gctttggtcg
      841 ggatggtggt gttcttctga atcagtttgg tcatcacgcc gcccatggtt tcgataccca
      901 aagacagagg agttacgtcc agtagcaata cgtcgctgcg gccgccgctc aatacttcgc
      961 cttggatcgc tgcgcctacg gcaacggctt cgtcagggtt cacgtctttg cgcggttctt
     1021 tgccgaagaa gtctctcacg gcttcttgta ctttcggcat acgggactgc ccgccgacca
     1081 agattacgtc gtcgatgtcg ccggtgctca agccggcatc tttcaatgca attttgcaag
     1141 gttcgataga gcgggtaatc aggtcttcaa ccaggctttc gaatttggcg cgggtaattt
     1201 tcatcgccaa gtgtttcggg ccggttgcgt ccatggtgat gtacggcagg ttaatttcgg
     1261 tttgctggcc gctggacaat tcgattttgg ctttttcggc agcttctttc aggcgttgta
     1321 gagccatcac gtcttgtttc aaatcaatgc cttgttcttt tttgaactcg gcgatgatgt
     1381 ggtcgatgag gcgttggtcg aagtcttcac cgcccaagaa ggtatcgccg ttggttgcca
     1441 atacttcgaa ttgtttgtcg ccgtcgaggt tggcgatttc gatgatggaa atatcgaaag
     1501 taccgccgcc caagtcatat acggctactt tgcggtcttt gttgtcgcct ttgtccatac
     1561 cgaatgccaa agcggctgcg gtcggctcgt tgatgatgcg tttcacgtcc aaaccggcga
     1621 tacggcctgc gtctttggtg gcttgacgtt ggctgtcgtt gaagtaggca gggacggtaa
     1681 tcacggcttc ggttactttt tcgcccaagt aagcttcggc ggcttctttc attttacgca
     1741 ggacttctgc ggaaatttga ggaggagaca gctctttgcc ttgtgctttt acccatgcgt
     1801 cgccgttgtt ggctttgatg atttcgaaag gcatagattc gatgtcgcgt tggacttctt
     1861 tgtcttcaaa tttgtggccg atcaggcgtt tggcggcgta aatggtgttt ttagcgttgg
     1921 ttacggcttg gcgtttggca ggcgcaccga cgaggatttc gccgccgtcc aaataagcga
     1981 taacggacgg cgtggtgcgt gcgccttctg cgttttcgat cactttggtt tgaccgtttt
     2041 cggaaatggc caaacaagag ttggttgtac ctaagtcgat accgattact tttgccatgt
     2101 ggataatcct atttgatttt gcttattttg agaaatatgt tggaacattt tgtcccgatg
     2161 ggctgtaaat agggcgggcg gcgggctgtt tcaagctaca gcatggctat aagtatataa
     2221 ctttatgaat atattggttt tatatttgat ttaatacatt tggctccaat gcatttaagc
     2281 ataatatttc aaatggcagg caggtttatt catagacgat gccggcgagc atttcctgtt
     2341 cgttcaagtt gccgtactct ttttcccagt cgtgtgaaga ctcgatgatg tcgcattctt
     2401 tggaaaggga gacttgttct gcatccatat ctttggcgtt cagtatgttg aattgttcgc
     2461 acagggatgc ggataaagtg atgtcaggct gtttggcttc agaacggttt tcttggaagg
     2521 caaagcagaa tgcggtaaat gccgcagcat agataagata tttgccggtt ttcttcattt
     2581 tctatccttt ttctgtcaac tcaggattaa acctatggaa aatcttaaaa attatgtatt
     2641 gagtaagaaa aatcataatt taaatttagt ttatcatagt tgtttcgttt ttgggatagc
     2701 taaggtaaaa tatatttcat gtttacttta gatgattgaa tgaaggggag cggaaggata
     2761 tttatggaaa cctttaaaga cagactggtt tttttatgga aaagcgaagc gaagcaggca
     2821 aaaatcgcat ccgatattga aatgacgatt gcgggcttca gcaggatatg gaatgaaggc
     2881 ggtctgccga agtctgaaac attaaaaaaa atcaagcagt tgaaggggtg tagtatcgat
     2941 tggctgctga ccggggaggg taatccgttt ccggatgaag ccccaaaaaa atcccttgct
     3001 tacgatacct tgggaaatga agtcgatacg gacgagtttg tctttgtgcc gagatatgat
     3061 attcgggcgg ctgcgggata cgggcagttt gtcgatcatg aggaaccggt atttacaatg
     3121 gcgttcagac ggcattggat tgagaattat gttacccgcg atacgaaaaa cctgtctgta
     3181 atttccgtca agggggattc gatggagggg gttttgaatg acggcgattc gattttggtc
     3241 aatcatggtg aaaatacgcc gagggacggt ctgtatgtgt tgcggattaa tgaaaatctg
     3301 ctggttaaac gtttacagat tgtaccgggc gggattatca atgtgatttc tgcaaacgag
     3361 gcttatcctg cttttgaaat caatttgaac gatttgaccg atgatgtgga gattatcggg
     3421 cgtgtcgagt ggttcggcag gacgatttga gtttggggct tgaaattgca ggcggtcaaa
     3481 cttatctatt ggaacaattc ctttttcaaa ggcgaagcct gcttgccttt aaagggggtt
     3541 tgagagagaa tgcagaaaat attatattaa ggaataacac catgtcggat gaaagcccta
     3601 ttatttttac tgacagctgc tgtgccaaag ttgccgattt gattgccgaa gaaaacaatc
     3661 ccgatttgaa attgcgggtt tttgtcaatg gcggcggctg ttcgggtttc cagtacggat
     3721 ttacttttga cgaaatcaaa aacgacgacg attttgaaat tgagaaaaac ggtttggtct
     3781 ttttggtcga tccgatgagc tatcaatatc tggtcggtgc ggaaatcgac tatacggaaa
     3841 gtttgcaggg ttcgcaattc gtcatccgca atccgaatgc ggaaacaacc tgcggttgcg
     3901 gatcgtcgtt ttccgtatga ccgcttggtt tgtgtgatgc cgtctgaacg ttcagacggc
     3961 atttttactt ttggaaaata tattatcggg atgaattcac atataatccg attgtttgaa
     4021 gatgaatcgg gtttcccgaa aggaacgggc ggaacggtat caggcgtatt tgttccctta
     4081 tgattgagat gagtaaagat taccgaaatg atttgtacga tgtatatgtt tcctacccgc
     4141 cccaagtgga tcgcgggctt atccgggagt gcctcaggga gaatctcggc gaggaaaagg
     4201 cggaaggatt gatcgaatcg ctcgattcca aacctcaagt gctggttgag gaaaaatgca
     4261 cttgggcgaa acgggaagag ttgcatgatt atttcagcta tttgggtttg gatattatta
     4321 cccgga
//
LOCUS       DAXHMX010000157         4258 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHMX010000157 DAXHMX010000000
VERSION     DAXHMX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.157"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            536..2263
                     /gene="ilvB"
                     /locus_tag="ACFM4S_001886"
     CDS             536..2263
                     /gene="ilvB"
                     /locus_tag="ACFM4S_001886"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6051510.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            2274..2765
                     /gene="ilvN"
                     /locus_tag="ACFM4S_001887"
     CDS             2274..2765
                     /gene="ilvN"
                     /locus_tag="ACFM4S_001887"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6051511.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2832..3125
                     /locus_tag="ACFM4S_001888"
     CDS             2832..3125
                     /locus_tag="ACFM4S_001888"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6051512.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3205..4218
                     /gene="ilvC"
                     /locus_tag="ACFM4S_001889"
     CDS             3205..4218
                     /gene="ilvC"
                     /locus_tag="ACFM4S_001889"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6051513.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRTDLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1107 a         1021 c         1076 g         1054 t
ORIGIN      
        1 caatatatta agtcgttgtt agccctcttg gttctgtcaa tgccgtccaa tacagtccgg
       61 tgcaatgagc cattttcagg aagtatggtt gggaatattt ttgatatatc aaaaataaaa
      121 tcaagttcaa tcaatatccc gagtctttat tgggatgaaa ccatgtaaat tttacaattc
      181 ttgatatcga agaagggttt ttcacatctg cccctcctgc ataaactgtc aacaatttcc
      241 atctttcaaa aaaggtactc ctgattttat tcttttaaaa tcaatcaata atttctctgc
      301 tttcaaaaaa tattaaaaga tgaaagattt ttcttgattg aactgtcggt ttgcgttatc
      361 gtttctactc tttttcacaa actctgtgtt cctttccaat tgattggata atccgtctgt
      421 tgccgtgttc ccccaatgcg cgtaccctaa atcgctgctt ggtggaattg cattacaggt
      481 gctgtggcaa agcggtttgt ccctattggt ttgaaaccgt ataaaagagg tcgttatgca
      541 gttatcaggc gcgcaaatca tagtgcagag tctcaaagcc gaaggtgtcg agtacgtttt
      601 cggttatccc ggcggtgcgg ttatcgaaat ctacgatgcc cttttccaac tcaataaatt
      661 caagcacatt ctgacccgtc acgagcaggc ggcagtacac gcggcagatg cgtatgcgcg
      721 cgtcagcggt aaggtgggcg tggcattggt tacatccggc ccgggcgtta ccaatgcact
      781 gaccggtatt gctactgcct atacggattc gattccgatg gtggtcatca gcgggcaggt
      841 aggcaattcc ctgattggta cggatgcgtt ccaagaagtt gatacggtgg gtattacccg
      901 tccgtgcgtc aaacacaatt tcctggttac ggacatcaat gagttggcgg aaaccattaa
      961 aaaggcgttc caaattgccg caagcggccg accggggccc gtggtggttg atgtcccgaa
     1021 agatgttacg caggcgatgg cgaaattcag ctatcctcag gaagacattt ttatccgttc
     1081 gtatcaaccg gttgttcaag ggcatatcgg tcagattaaa aaggccgtgc agatgttggc
     1141 atctgccaaa cgtccggtcg tctattttgg cggcggcgtg gtgttgggta atgcttctga
     1201 agagctgacc cgatttgtcc gaatgacggg tgctccgtgc acgggtacgc tgatgggctt
     1261 gggcgcttat ccttccggcg accgccaatt cctcggtatg ctcggtatgc acggtactta
     1321 cgaggcaaac cttgccatgc agaatgcgga tgtcgttctt gccgtaggtg cgcgttttga
     1381 cgaccgtgtg gtttccgtac cgtccaaatt tttcgagaag gcgaagaagg ttatccatat
     1441 cgatgtcgat ccttccagca tcgccaaacg cgtgaaggcg gacattccga ttgtcggcga
     1501 cgtgaaaaac gttttgtccg agatggttgc gctgtggcaa aaacaagagt ccgtgccgtc
     1561 tgaagatgct ttgggcaaat ggtggaaaac catagaggaa tggcgttccc gcgattgctt
     1621 gtggtttgac aacggcagcg aaattatcaa gccacaatat gtgattcaga agcttgccga
     1681 gattaccggc aattcggcaa tcatcacatc ggatgtaggg cagcatcaaa tgtttgcggc
     1741 tcaatattat cccttcgaac gtccgcgcca atggctcaac tccggcggtt tgggtacgat
     1801 gggcgttggt ctgccttatg cgattggtgc aaaacttgcc gccccggatc aagacgtatt
     1861 ctgtattacc ggcgacggct cgatccagat gaacatccaa gagctgtcca cctgtttcca
     1921 atatcggatt ccggttaacg tcattacgct gaacaacggt tatctcggta tggtacgcca
     1981 gtggcaggaa atatattacg gcggccgaga gtcggaaact tatttcgatt ctttgcccga
     2041 tttcgtcaaa cttgccgagg catacggtca tatcggcatc cgcgtggaca agaagtctga
     2101 tgtggaaggt gcgttgttgg aagcattgaa ccaaaaagac aggctggtgt ttatcgactt
     2161 cctgaccgac cagaaacaga atgtgatgcc catggtcggt aacggcaaag gtttggacga
     2221 aatggtactt ccgccgcata tgcgtgcgga cgaaaaggcg taaggagagg caaatgcgac
     2281 atatcttatc tgttctgatt gaaaacgaat caggtgcgat gagccgcgtg gtcggtttgt
     2341 tctctgcacg cgattacaat atcgattctt tggcggttgc gccgaccgaa gacaaaaccc
     2401 tgtcccgtat gaccatcgtt acccacggcg acgagcaggt catcgaacaa atcaccaagc
     2461 aactcaataa attgattgaa gtgattaaag tggtcgattt gaacgaaagc cgttttgtcg
     2521 aacgcgaact gatgttggta aaagtccgtg ccgccggcaa agaccgcgac gaatttttac
     2581 gcttgaccga aatctaccgg ggcagcatca tcgacgtaac cgaccgcagc tatacgattg
     2641 aaattaccgg ctcgacagac aagctggatt cctttttgga aacggtcgga cgcgcccaaa
     2701 ttttggaaac cgtacgcaca ggcgcggccg gcatcggtcg cggtgagcgt attttgaaaa
     2761 tttaacgccg taacctttca gacggcacgg tatttgaatg tcgtctgaaa aacgaacggc
     2821 aggagagatt tatgtcgaac attaaaattg tcgcactggt taccgtcaaa ccggaataca
     2881 cagaaacact ggcagcacag tttaaagaac tggtcaaagc cagccgtgca gaagagggca
     2941 acatcagcta cgatctccat caggaaatcg gcaaaccgaa ccgttttgtt ttcgtggaaa
     3001 attggaaatc tcaagcagct attgacgaac ataacgccag cgcgcatttc caagccttcg
     3061 tccagtccgt cgacggcaaa accgaagcgt tggaaatcgt attgatgaat gaagttgccg
     3121 attaagcggc acactctgtt taacccgtcc gaagccgctc aacattttaa ggcttcgaca
     3181 accatttacc ttaaaggaaa tcaaatgcaa gtctattacg ataaagatgc cgatctgtcc
     3241 ctaatcaaag gcaaaaccgt tgccatcatc ggttacggtt cgcaaggtca tgcccatgcc
     3301 gccaacctga aagattcggg tgtaaacgtg gtgattggtc tgcgccaagg ttcttcttgg
     3361 aaaaaagccg aagcagccgg tcatgtcgtc aaaaccgttg ctgaagcgac caaagaagcc
     3421 gatgtcgtta tgctgctgct gcctgacgaa accatgcctg ccgtctatca cgccgaagtt
     3481 acagccaatt tgaaagaagg cgcaacgctg gcatttgcac acggcttcaa cgtgcactac
     3541 aaccaaatcg ttccgcgtac cgacttggac gtgattatgg ttgcccccaa aggtccgggt
     3601 cataccgtac gcagtgaata caaacgcggc ggcggcgtgc cttctctgat tgccgtttac
     3661 caagacaatt ccggcaaggc caaagacatc gccctgtctt atgcggctgc caacggcggc
     3721 accaaaggcg gtgtgattga aaccactttc cgcgaagaaa ccgaaaccga tctgttcggc
     3781 gaacaagccg tattgtgcgg tggcgtggtc gagttgatca aagcaggttt tgaaaccctg
     3841 accgaagccg gttacgcgcc tgaaatggct tacttcgaat gtctgcacga aatgaaactg
     3901 atcgttgacc tgattttcga aggcggtatt gccaatatga actactccat ttccaacaat
     3961 gcggagtacg gcgaatacgt taccggccct gaagtggtca atgcttccag caaagaagcc
     4021 atgcgcaatg ccctgaaacg catccaaacc ggcgaatacg caaaaatgtt tatccaagag
     4081 ggtaatgtca actatgcgtc tatgactgcc cgccgccgtc tgaatgccga ccaccaagtt
     4141 gaaaaagtcg gcgcacaact gcgtgccatg atgccttgga ttactgccaa caaattggtt
     4201 gaccaagaca aaaactgatt gttttcaaac gggactgcct acacatcgtg taggcagt
//
LOCUS       DAXHMX010000158         4243 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHMX010000158 DAXHMX010000000
VERSION     DAXHMX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.158"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..571
                     /locus_tag="ACFM4S_001890"
     CDS             <1..571
                     /locus_tag="ACFM4S_001890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6051514.1"
                     /translation="FHFRKTKKECAVLGTLSRLDTVRAVHLMLDILKKMVDRGMPVRL
                     NVAGIGEEMDNLKAQAKRLGIEDKVTFLGGVRDLTGYFKEVDILVNTPHCVGDHGAGV
                     GNNILEAGLYDTPVVTYNMAGISEMVITGQTGYCIPFGDDEAFIEAVDTLIKHPELRS
                     QMGKALHKHVETLCSDDEIYRTTMAAYEM"
     gene            585..1757
                     /locus_tag="ACFM4S_001891"
     CDS             585..1757
                     /locus_tag="ACFM4S_001891"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6051515.1"
                     /translation="MNITIVAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVMLLEESGYSKNVSLGRVTSHHRFVKHFEKWLENCRPGE
                     QDVVFSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKETNPNVPGEVIYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLEKLKQYENRAIKFYGY
                     LPYSEMMSIAKGCDIAVNAIHSHAMQSVTNKLSDYMALQKPVLNSQTNAEVLDLMNLL
                     PHENYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFRRDVAYQKIVNLIERLADE"
     gene            1750..2277
                     /locus_tag="ACFM4S_001892"
                     /pseudo
     CDS             1750..2277
                     /locus_tag="ACFM4S_001892"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            2326..3327
                     /locus_tag="ACFM4S_001893"
     CDS             2326..3327
                     /locus_tag="ACFM4S_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFC6051516.1"
                     /translation="MPYFTGNQTNEKNNILILSAGRRVELVQDFQTEAARFSDGISVF
                     ATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRENIGLIIPTIDTELQKLAD
                     ERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPY
                     DGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAI
                     PRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCITVQFFYNEETGEFYGVEI
                     NPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            3314..4000
                     /locus_tag="ACFM4S_001894"
     CDS             3314..4000
                     /locus_tag="ACFM4S_001894"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6051517.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            complement(4063..>4243)
                     /locus_tag="ACFM4S_001895"
     CDS             complement(4063..>4243)
                     /locus_tag="ACFM4S_001895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051518.1"
                     /translation="LIAYAVPVLGFLRGSGFAGLPATVMPSGMSHTITAPAPIQLPSF
                     PNGVENVGRKAVYAP"
BASE COUNT         1082 a         1193 c         1004 g          964 t
ORIGIN      
        1 atttcatttc cggaaaacca aaaaagaatg cgccgtactc ggcacacttt cccgtttgga
       61 caccgtccgc gccgtgcatc tgatgttgga tattttgaag aaaatggttg accgcggtat
      121 gcctgtacgc ctcaacgttg caggtatcgg cgaagaaatg gacaacctca aagcccaagc
      181 caaacgtttg ggcattgaag acaaagtcac cttcctcggc ggcgtccgcg atttgaccgg
      241 ctacttcaaa gaagtcgata ttttggtgaa cacgccgcat tgcgtgggcg atcacggcgc
      301 aggtgtcggc aacaatattt tggaagccgg cctttacgac acgcctgtcg tgacctacaa
      361 catggcgggc atttccgaaa tggtcattac cggccaaacc ggctactgca ttcctttcgg
      421 cgacgacgaa gcatttatcg aagccgtcga tacactcatc aagcatccag agttgcgcag
      481 ccaaatgggc aaagccctgc acaaacatgt cgaaacctta tgctccgacg acgaaatcta
      541 ccgcaccacc atggctgcgt acgaaatgta aggccgtctg aaacatgaac attaccatcg
      601 tcgcccctta ttgctcgctg ccgtccgagc cgcatttcaa ccgcttctgg tatcttgccg
      661 agctgttgtc gcagtcgcat gacgtgttgc tgattaccag caacttcaag cactacgaca
      721 aatctttcag acggcccgaa gatgccgagg ccgcctcaca aggccgtctg aaagtcatgc
      781 tgttggaaga aagcggatac agcaaaaacg tgtctttagg acgcgtgacc agccatcacc
      841 gcttcgtcaa acattttgaa aaatggttgg aaaactgccg tccgggcgaa caagacgtcg
      901 tcttttccgc ctatccgctg attgccacca acctgctgtt gggcaaacac aaagcgcgtt
      961 tgggctacaa actgattgtc gatgtgcagg acgtatggcc ggagtctttc tcctcggtcg
     1021 taccgttttt gaaaaaagta ccgcacaagc tgctgccctt tgcttcacgc gccaaccggg
     1081 cctaccgcta cgccgacgcg ctggttgccg tatcgcagac ttatctcgac cgcgccaaag
     1141 aaaccaatcc gaacgtaccc ggcgaagtca tctatattgg tgctgacttt cccaaactcg
     1201 atgccgcacc tgccaaagat tttggcgacg acaaaacccg ctttttctac ttgggcacgc
     1261 tcagttacag ctatgacgtg gaaaccgtgt gcaaaggcgt gcggaaactt ttagacgacg
     1321 gcgaaaacgt cgagttgcac atcatgggcg gaggacccga tttggagaaa ctcaaacaat
     1381 atgaaaatcg cgccatcaag ttttacggtt atttacccta ttctgaaatg atgtcgattg
     1441 ccaaaggctg cgacattgcc gtcaacgcca tccatagcca cgccatgcag tcggttacca
     1501 acaaactctc cgactatatg gctttgcaaa aacccgtgct gaacagccag accaatgccg
     1561 aagtcctcga cctcatgaat ctgctgccgc atgaaaacta ccgttccggc gacgtggaca
     1621 gcttcgttca agccgccaaa gatattttga agcgcaaaaa cgaccccgtt cagtccgacg
     1681 aaatcgtccg ccgcttcagg cgcgacgttg cctatcaaaa aatcgtcaac ctgattgaaa
     1741 gattagctga tgaataaatt tttcaaacgc ctgttcgaca ttgccgcctc cgcatcgggg
     1801 ctgattgtcc tctcacccgt tttcctgatt ttggcgtacc tcatccgcaa gaatctgggt
     1861 tcgcccgtgt tcttcatcca agagcggccg ggcaaggacg gcaaaccgtt taaaatgatt
     1921 aaattccgct ctatgcgcga cgcgcttgat tcagacggca ttccgctgcc cgacagcgaa
     1981 cgcctgaccg atttcggcaa aaaactgcgt gccgccagtt tggacgaact gcccgaactg
     2041 tggaatgtct taaaaggcga gatgagcctg gtcggcccgc gcccgctgct gatgcaatat
     2101 ctgccgctgt acgacaactt ccaaaaccgc cgccacgaaa tgagaccggg cattaccggc
     2161 tgggcgcagg tcaacgggcg caacgcgctt tcgtgggacg aaaaattcgc ctgcgatgtt
     2221 tggtatatcg accattacag tttttggctg gatatgaaaa tcctgttttt gacagtcaga
     2281 aaagtcttga ttaaagaagg tatttcggcg gagggcgaag ccactatgcc ttatttcact
     2341 ggaaatcaaa ctaatgaaaa aaataatata ctgattctgt cggcaggtcg ccgtgtcgaa
     2401 ttggtgcagg acttccaaac cgaagccgca cgtttttcag acggcatcag cgtttttgcc
     2461 actgacctca acccccgcat gtcttctgcc tgccatgttt cggacggttc atttgaagtg
     2521 ccgccgatta gtaccgattc atatatcgac agcattttca atctggcggt acgcgaaaat
     2581 atcggtttaa tcatcccaac catagacacc gaactgcaaa agctggcaga cgagcgcgaa
     2641 cgctttgaag cggcaagtgt ccacatcgtc gtttccgatt cggacttcat cgcacaatgc
     2701 cgcgacaaac ggaaaaccgc caccttgttt gcccgctacg gcatacgctc acccgaaatt
     2761 tacgaccgca agaagttggt tttcccttgc ttcgccaagc cttatgacgg cagccgcgcc
     2821 atcggtgcaa aacggattga cacacctgcc gacctgacac cggaagtctt ggcagacacc
     2881 aaattaatgt tctgtcagtt tatcgacatc gaaaataaat tttccgagtt taccgtcgac
     2941 atgtattacg accgtcaatg ccgtctgaaa tgcgccatcc cgcgaaaacg cttggaagtg
     3001 cgctccggcg aaatcagcaa aggcgttacc tgcaaaaaca gcctctacca aacgctgctg
     3061 gaaaaaatgt cggtattgca gggcgcgcgc ggctgtatca cggtgcagtt cttctacaac
     3121 gaagaaaccg gcgaattcta cggcgtggaa atcaaccccc gcttcggcgg tggcttcccg
     3181 ctgacgtatg ccgcaggcgg caattatccg agccggttga tgcgcgaata cctcggcgac
     3241 gaagaaatcc cgttttcaga cgactgggaa aacaacctca ttatgttgcg ctacgatgcc
     3301 aaggtcttgg ttcatgaaaa cgattaatcc tgaaaccacc gtcatcgtct tcgacttgga
     3361 cgataccctg tattcggaat acgaatacaa gctctccggt atccgttcgg tcatcgatac
     3421 cgttgccgca ctgtatcccg attggaattc agacaaccta tggcgcaaca tcgaccccga
     3481 cggcaaagat tggctggaca aattgtgccg ccactgtggt tttaatgaat cggaaaaaca
     3541 ggttcttttg tggcaatacc ggctgcaccg cccgacgctg actccgtatg ctccgccgga
     3601 tttcctgtcc gaactgaccg cgcccttcgc cgcacgcgcg ctgattaccg acggcagaag
     3661 cctgacccaa cgcctgaagc tggaagcctt gggtctgtct tccctgtttg acgacatcct
     3721 catctctgaa gcctgctctt cggaaaagcc cgacggcaaa cgcttccgcc atcttcaaga
     3781 caaatatgcc gacaaggcag gctgcttcat ctatatcggc gacaacattt ccaaagactt
     3841 tatcgcgccg aacactttgg gctggataac cataggattg cttccctccg gacagaacat
     3901 ccaccgccac cagcctgaaa gttttaacac agaatatcat cccgactttt gggttaactc
     3961 ccttcaagat ttagttccta taattgattc aaatacttaa ccattacata atcaaacatg
     4021 cactcacaat attaaaagga ctatttcatg acaaaatatt attcatgggg cgtaaacggc
     4081 tttccgccct acgttttcga caccatttgg aaacgatgga agttgaatgg gcgcgggggc
     4141 ggttatcgtg tgcgacatcc cggacggcat gaccgtcgcg ggcaatccgg caaagccgct
     4201 gccgcgcaaa aaccccaaga ccgggacggc ataagcgatt aaa
//
LOCUS       DAXHMX010000159         4156 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHMX010000159 DAXHMX010000000
VERSION     DAXHMX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.159"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            337..1854
                     /locus_tag="ACFM4S_001896"
     CDS             337..1854
                     /locus_tag="ACFM4S_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6051519.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRLVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNQNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1915..3795)
                     /locus_tag="ACFM4S_001897"
     CDS             complement(1915..3795)
                     /locus_tag="ACFM4S_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6051520.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDSALQKGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAENWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPISLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNLASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIETLETEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLKPASASDGIVSGIGGPDSNVQARTAGQGEATPYRFQVHPLYQGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETMRVENRQCALLDRHLCRLKAAAQALN
                     LPLPDGCENQIKQYIADLPDGAFRVKALLASDGISLSRAVLNHLADKQRVIISPAVLP
                     AQNYLRRFKTTHRTLFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          870 a          935 c         1350 g         1001 t
ORIGIN      
        1 tttgcgggaa tgacggcgac agggttgctg ttatagcgga ttaacaaaaa ccagtacggc
       61 gttgcctcgc ctcagctcaa agagaacgat tctctaaggt gctgaagcac cgagtgaatc
      121 ggtttcgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
      181 gctataaatg tgaaatccgc cctttgaaaa tcgggcggcg gttttttgcc tgcctgcttt
      241 cagacggcaa agccggtttt cacgggtttc tgcctgtttt tcggatggtt tgacgtgctt
      301 cggcggcgtg tttgccagaa aggtaaatga cagggtatgt tgtatttcag atacggtttt
      361 ttggttgttt ggtgtgcggc aggtgtttct gccgcctatg gggcggatgc gcccgcgatt
      421 ttggatgaca aggcattgtt gcaggtgcaa cggttggtgt cggataagtg ggcggaatcg
      481 gattggaaag ttgaaaatga tgccccgcgc gtggttgacg gggatttttt gttggcgcat
      541 ccgaaaatgt tggaacatag tttgcgcgac gcgctcaacg gcaatcaggc ggatttaatc
      601 gcttcgttgg cggatttgta tgccaagctg ccggattatg acgcggtttt gtacggcagg
      661 gcgcgggctt tgctggcgaa attggcggga aggccggcgg aggcggtggc gcggtatcgg
      721 gaactgcacg gggaaaatgc ggcagacgag cggattttgc tggatttggc ggcggcggag
      781 tttgacgatt tccgactgaa gtcggcagaa aggcattttg cggaggcggc aaaattggat
      841 ttgccggcac cggttttgga aaatgtgggg cgttttcgga aaaaaacgga ggggctgacg
      901 ggctggcgtt tttcgggcgg catcagtccg gcggtcaatc aaaacgccaa taatgccgcg
      961 ccgcagtatt gccggcaaaa cggaggccgg cagatatgca gtgtcagccg ggcggagcgg
     1021 gcggcagggt tgaattatga aatcgaggcg gaaaaactga cggcgttggc agataatcat
     1081 tatttgttgt tccgttccaa tatcggcggc acgagctatt atttcagtaa aaaatcagct
     1141 tatgacgacg ggttcggcag ggcgtatttg ggttggcagt ataaaaatgc acggcagacg
     1201 gcggggattt tgccgtttta tcaggtgcag ttgtcgggca gcgacggctt tgatgcgaaa
     1261 acaaaacggg taaacaaccg ccgcctgccg ccgtatatgc tggcgcacgg agtcggcgtg
     1321 cagctgtccc atacttaccg accaaacccg ggatggcaat tttcggtcgc gctggaacat
     1381 taccgccaac gctaccgcga acaggatagg gcggaataca ataacggcag gcaggacggg
     1441 ttttatgttt cgtcggcaaa acgtttgggc gaatcggcaa ctgtgttcgg cggctggcag
     1501 tttgtgcggt ttgtgccgaa acgcgaaacg gtgggcggcg cggtcaataa tgccgcctac
     1561 cggcgcaacg gtgtttatgc cggttgggcg caggagtggc ggcagttggg cggtttgaac
     1621 agtcgggttt ccgcgtctta tgcccgccgc aactataagg gcgttgcggc tttctcgaca
     1681 gaggcgcaac gcaaccgcga atggaatgtc tcgctggctt tgagccacga caagttgtcg
     1741 tataaaggta tcgtgcccgc gttgaattat cgtttcggca ggacggaaag taatgtgccg
     1801 tatgcgaaac gccgcaacag cgaggtgttt gtgtcggcgg attggcggtt ttgaatggtg
     1861 gaataatgcc gtccgaactt tgtggaaaca ggttcggacg gcatttttgc gcgttcaggc
     1921 aagggcggcg gcaaatacgc cgcgcaaggc gttggagagg cggatttctt cggcttcttg
     1981 cagtgttttt tgtgtgatgt gtgtttcgat tacttgattt gtttgcaaat atttttgcgg
     2041 ttcgtccaac acggcttggc gcattatgcc gtttaaaatg tctaaatcta aagagggcgt
     2101 gagccattgt ccgcgatgtt tgacgaacac gttgcttctg ccgccttcga gcaggatgcc
     2161 gtctgaattg aaaaacaggc tgtcgaacgc gccttgtgtt tcggcggttt gccacgcttg
     2221 gtcgaagaga gtgcggtggg tggttttgaa gcggcgcagg tagttttgtg cgggcaggac
     2281 ggcgggcgaa atgatgacgc gttgtttgtc agccaaatgg tttaaaacgg cgcgggacag
     2341 gctgatgccg tctgaagcga gcagggcttt gacgcggaac gcgccgtcgg gcaagtcggc
     2401 aatgtattgt ttgatttgat tttcgcagcc gtcgggcagg ggcaggttga gggcttgggc
     2461 ggcggctttc agacggcata ggtggcggtc gagtagggcg cattgcctgt tttccacgcg
     2521 catggtttca aaaatgccga agtcggggcg caattcgttg aggaaacggg ctttccagcc
     2581 gcattcgcga tattcggcgg cgggatcgct gtcgatgacg atgccggaac cgacgccgta
     2641 caccccttga tagagtggat gaacttgaaa ccggtacggc gttgcctcgc cttgccctgc
     2701 cgttcgggct tgcacgtttg aatctgggcc gccgatgcct gaaacaatgc cgtctgaagc
     2761 cgaagcaggt ttgagcgaca aggtgcggat aacgacgttg aacgtgcctt caaaccccaa
     2821 gccgccggaa cacgggttca aatagccaat gctgcccgta taaagtccgc gcgcttcggt
     2881 ttcgagcgtt tcgataatct gcatactcat ttttttgggc gcgccggtga tgctgccgca
     2941 ggggaaggcg gcgcggagga tgtcggcgaa cgaggtgtgc ggcaaggctt gggcttggat
     3001 ggtgctggtc atctgccaga cgctgccgaa acgcgatact ttaaacggct cgggtacgca
     3061 tactttgccg gtttgggcga ttttgccgag gtcgttgcgc agcaaatcga caatcatcac
     3121 gttttcggcg cggtttttcg ggtctgcttg caactcggcg gcgcggcgtt cgtcttgtcc
     3181 gtcgcccaaa atcggcgcgg tgcctttcat cggttcggtg ctgatggtgc cgtctgaagc
     3241 gaggttgagg aagagttcgg gcgagaaaca cagcgtccac gcggattgcc cctccgcatc
     3301 gggcaggtgg gacaagacgg cataggggac gggctggcgc aggcggcggt agaggctgat
     3361 gggattgccg taggcttgca ggtgcaggcg ggtggtgtag ttgatttgat aggtgtcgcc
     3421 gcgccggatg gcttcgtgga tttggcggat atggtcgagg taatcggctt cggatacgga
     3481 ggattgcggc gtggaaatgc cggcggggag gccgtctgaa tgtcgggcaa gccagttttc
     3541 ggcatcggtg tcggcgcagt cggcaaacca gtgcagggca agattgccgc cgcgttcgga
     3601 ctcgaccccc gtcagcggca aaccgaatcc gtagtctgca aacaacaccg aatgcagccc
     3661 tttttgccag cccttttgca gcgcgctgtc caaagcatcg agttcttcgg gacggaaaaa
     3721 acggctttcc acatgatttt gatagcgttt tgcgcggccg cttacggcat cgtcaaacag
     3781 ggcgaaataa ggcatggcaa ttcggggcaa atgttttgat tatacgctct ttttacacat
     3841 attttcagac gattcggcaa atatcggcaa aatgtaattt tatgtagaga aagtgggggg
     3901 cgaaggtgta aaattgtgaa aacaaattcc gatttccaac ctgaaataca ataaggagac
     3961 ctttatgaca gaccaccagc tgcaaccgtt tgaaaacgta gaattaggcg aaaagcaaga
     4021 ccagctccaa gtatagtgga ttaacaaaaa ccagtacagc gttgcctcgc tttagctcaa
     4081 agagaacgat tctctcaggt gctgaagcac caagtgaatc ggttccgtac tatctgtact
     4141 gtcttcggct tcgttg
//
LOCUS       DAXHMX010000160         4058 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHMX010000160 DAXHMX010000000
VERSION     DAXHMX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.160"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1169..1606)
                     /gene="rnhA"
                     /locus_tag="ACFM4S_001898"
     CDS             complement(1169..1606)
                     /gene="rnhA"
                     /locus_tag="ACFM4S_001898"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6051521.1"
                     /translation="MDKTVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1720..2589
                     /gene="tehB"
                     /locus_tag="ACFM4S_001899"
     CDS             1720..2589
                     /gene="tehB"
                     /locus_tag="ACFM4S_001899"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC6051522.1"
                     /translation="MKEWIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCVAEDYFH
                     KKYGMSATHSAVAAAQDIVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKRPE"
     gene            complement(2639..3445)
                     /gene="thiD"
                     /locus_tag="ACFM4S_001900"
     CDS             complement(2639..3445)
                     /gene="thiD"
                     /locus_tag="ACFM4S_001900"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6051523.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          871 a         1011 c         1141 g         1035 t
ORIGIN      
        1 ggtttttgtt aatccactat agtttaattt gtctgtccgc attgccgcta attgtaccac
       61 ttcatcgtat ttccttttcg gttgaaaccc cgccctttag ggcggcagga tcagacttta
      121 tttgggaggg ggtgtaaccc cttccaaatc aggacgacac atagggaggt gctttatgtg
      181 tcgtcctgtg tgttgaaata tatcggacgc gtttgaacag ttgtgcggca aactgtttta
      241 gcggcaggca agagatgccg tctgatgccg tctgaagccc attgtccgtc attcccacac
      301 aggcagttgc tacggttgct gaagcccctt agccgtcgcg caggcggttg ctacggttac
      361 taaaatcccg tcattcccgc gcaggcggga atccagtccg ttcggtttca gttgtttctg
      421 ataaatcttg cagcgttggg tttttagatt cccgctttcg cgggaatgac ggggcggtcg
      481 gatgccgcct gaaactccgt cattcttgca acttttcgtc attcccgtga aagcgggaat
      541 ctagaccttt taaattttca cacggctttt gaatattgcc gttacccgaa ggtctagatt
      601 cccgcttgcg cgggaatgac ggtagagcgg tttcagttgc tcccgataaa tgccgcaatc
      661 tcaaatcccg tcattccctc aaaaacagaa aaacaaaatc agaaacctaa aatcccgtca
      721 ttcccgcagc tcttcgtcat tcccgtgaaa gcgggaatct agacctttta aattttcaca
      781 cggcttttga atattgccgt tacccgaagg tctggattcc cgcctgcacg ggaatgacgg
      841 cagagcggtt tcagttgttt ctaaataaat ttttgcagcg ttggattttt agattcccgc
      901 tttcgcggga atgacggggc ggtcggatgc cgcctgaagc ccgacaaagc ccccaatgcc
      961 gtctgaaacc tcgtcattcc tctgctgtcc gcaacttttc gtcattcccg caaaagcggg
     1021 aatctagagc ctctaaattt tcagacggct tttgaatatt gccgttgccc gacggtttgg
     1081 attcccgcct gcgcgggaat gacggtggag gacgatgccg tctaaagccc attagcggtt
     1141 tcagacggca tttttgccgg agcggcagtc aggaaaactg cgctgcgcca cggtttgcca
     1201 aatcgtcggc gcgttcgttt tccgcgtgtc ccgcgtgtcc tttaacccaa gtccagctga
     1261 cttggtgctg cccaaccaga acgtcgagtt ctttccacaa gtcgtcgttt ttgacgggct
     1321 gtttggcggc ggttttccag ccgttgcgct tccaaccgtg tatccagttt tccatgccgt
     1381 ttttgacgta ttgcgagtcg gtgcagatga tgacggtgca gcggcgtttg agcgatttca
     1441 gtccttcgat gacggcggtc agctccatgc ggttgttggt ggtttgcgct tcgccgccga
     1501 aaagttcttt ttcgcggctg ccgtagcgca tcaacacgcc ccagccgccc gcgccgggat
     1561 tgcctttgca cgcgccgtcg gtgtaaaggt aaacggtttt gtccattatt gtgcctttgt
     1621 gtggtgtccg gcgtgattat aagcggtttg tgcgccgtgc cgtctgaaag attgcggcgg
     1681 attaaaatag gcatataatg tgcaacttta tttttgaata tgaaggagtg gatagtgggt
     1741 caaagcggcg agttgttttg tttcggacag atgcccgtat ggaaggcgga aaacctgccg
     1801 gaagttttgt tgtcgggcta ttcgtccgaa ggaggggaat gggtctgcct gaatgtgttg
     1861 cagggcgatg tcgaagtccg cacgcaggac ggggcggcgg aggtttggtc ggcagaaagc
     1921 ggcgattgcg tgtttgcgcc gcagcaggtg ttttcggtca aaccgaaaac ggacgatgcc
     1981 gaaatccgtt tgtcgatgta ttgtgtggcc gaagattatt tccacaaaaa atacggcatg
     2041 agtgccacgc attctgcagt ggcggcggct caggatatcg tacctgcggg cagggcgctc
     2101 gatatgggct gcgggcaggg gcgcaacgcg ctgtttctcg gtttgaaggg gtttgaagtt
     2161 actgcagtcg atcacaatcc cgccgccttg gcaaacgtgg cggagctggc agaggcggag
     2221 ggtttgaacg tccgcacgct ggaatatgat ttgaacgccg ccgccttgca gggcgagttt
     2281 gattatattg tggcaacagt ggtgctgatg ttcctgatgc cgcagcgcgt acccgacgtg
     2341 attgccgata tgcaggcgca tacggcggcg ggcgggtaca acttgattgt atcggcaatg
     2401 gatacggcgg atttcccctg cccgatgccg ttccccttca aatttaaaga gggagaactg
     2461 aaggattatt atcgggattg ggagctggtc gaatataaag aagaattggg cgcgatgcac
     2521 gccaaagacg aaaacggcaa tccgatacgg tttaaatttg taaccatgct ggcgaaaagg
     2581 cctgaataat cttcaaaaat gccgtctgaa cattttgttt cagacggcat ttttacggtt
     2641 agtcccgata cgcccaatga ttgaccggcc cgtgtcccgc gccgatttcc aaagggtttg
     2701 agattgccgc cgtgatatag gccttggcag tctgtacggc ttcgcaaacg tccgagcctt
     2761 ttgccaattc agcggtaatg caggcggaaa acgtgcagcc tgtgccgtgc gtgtgggcgg
     2821 tcggaaagcg cgggctgtcc aattccagcg tttcgttttg cgtaaacagc caatccgtgc
     2881 agcgtccgct gctgctgccg ttcaaatgtc cgcctttgat aacgacattt ttgacaccgt
     2941 aatcaagcag gatttttgcc gcacgttccg catctttacg gttttcgata tgcacgccgg
     3001 tcagggcttc cgcctcgggc aggttggggg tcaatacatc cgtatcggga agcagcaggc
     3061 gcgtcagtgc cgcaacggcg gaatcctgca acagcggcgc accgcctttg gcaatcatca
     3121 cagggtcgag tacgcgcctg ccgaagtcac agtgtttcag cttgtcggca acgcattcga
     3181 tgatttccgc cgtgccgagc attcctattt tgtaggcgcg gatgtcaaag tcttccctga
     3241 ttgcttggat ttgtgcggtg atggtttcgg tcgggacgag atgaaccgcc gacacgccca
     3301 aggtattttg cgcggtaacg gcggtgatga cgcacgttcc gaacacgccg cgcatctgaa
     3361 acgttttcaa atccgcctga atgcccgcac cgccgcccga atccgaaccg gcgatggtaa
     3421 gcgtttgcac aaaagagctt ttcatttgtt cctccttaat attgtgtccg ataaaaaggg
     3481 ctaatgcctg taaaaaagca cggcgatgcc gtctgaaaac cgcagcgcgg ggtttgtggt
     3541 gcattatatg ccgtattgca ggacaaggca cagggcaggg cggcggggtg catcgtagca
     3601 actgtatttt tgcccgacgg ggtgaaaaat acagttgcta cacccactga taatgaaaaa
     3661 agagagcaat gccgtctgaa ccgcgtgttg cggcgagcat aaaccggcac gctttattcg
     3721 tcattcccac aaaaacagaa acctaaaatt ccgtcattcc tacgaaagcg ggaatccagt
     3781 gtgttcggtt tcagttgttt ttgggtttcg ggtgatttcc aaatcatcat tcccgcgcag
     3841 gcgggaatct agcccttggg gcagcggcaa tatttataaa gccgtctgaa aattcagaag
     3901 ttctagattc ccgttttcac gggaatgacg aagggttgtg ggaatgacga agtttcagac
     3961 ggcatcgccc cgccccgtca ttcccgcata agcgggaatc caaaccattg ggcagagcag
     4021 ccttaaaggt tataaaagac tcgtcattcc cacgaaag
//
LOCUS       DAXHMX010000161         4008 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHMX010000161 DAXHMX010000000
VERSION     DAXHMX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.161"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..632)
                     /locus_tag="ACFM4S_001901"
     CDS             complement(<1..632)
                     /locus_tag="ACFM4S_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6051524.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKHN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPSADFLELLSLCRDIHALTEGAFDPTVQVLWKLYADYFSAHPDAETPPPEN
                     SVKEALKRVGFGKVSFDDREIRFTEKGMGLSLNGIAQGYSGLTKTSTALPR"
     gene            complement(641..1435)
                     /locus_tag="ACFM4S_001902"
     CDS             complement(641..1435)
                     /locus_tag="ACFM4S_001902"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6051525.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            1858..3192
                     /gene="gdhA"
                     /locus_tag="ACFM4S_001903"
     CDS             1858..3192
                     /gene="gdhA"
                     /locus_tag="ACFM4S_001903"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6051526.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3334..3708
                     /locus_tag="ACFM4S_001904"
     CDS             3334..3708
                     /locus_tag="ACFM4S_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051527.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3752..>4008
                     /locus_tag="ACFM4S_001905"
     CDS             3752..>4008
                     /locus_tag="ACFM4S_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HFC6051528.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1090 a         1046 c          938 g          934 t
ORIGIN      
        1 cgaggcaacg ccgtactggt ttttgttaat ccactatagc cttgcgcgat gccgttcaag
       61 gacaaaccca tacccttttc ggtaaaacgg atttcacggt cgtcaaacga gactttgccg
      121 aagccgaccc gcttcagggc ttccttcacg ctattctccg gcggcggcgt ttccgcatcg
      181 ggatgggcgg aaaaataatc ggcatacagt ttccacaaaa cctgaaccgt cggatcgaac
      241 gcgccttccg tcagcgcgtg aatatcgcgg cacaggctca acagttccaa aaaatccgcc
      301 gacggcgaag tcagataacc gtcacggttc aggcggctga tcaggctgtc ttcacggtaa
      361 aggctgaaca ttttttccaa acgcgccact tccgccaaaa ccttattgac caaatccgcc
      421 gcctgtctgt cgtccacgcc gaacagacgc agctccgcac cggaacccag cgcgacacct
      481 ttccagaaaa acacattttc attgtgtttt tcatctttat tgcgtttttc atcatcggcg
      541 gcaaaaggat tcggcaaaaa agaaaccgcc gcgcccgccg ccgcaacggc ggcaactgtc
      601 aaaaaacgcc tgcgcccgaa atgcctgccc ataccgcctc ctaaaccgac actgccgcct
      661 tgatgtgcgg atgcggatcg tagccttcca actcaaaatc ttcaaatttg aaggcaaaca
      721 aatctttgac ttcaggattg attttcatca caggcaaggc gcgcggttcg cgttccaact
      781 gcaatgcggc ctgctcgaaa tggttgcggt acaggtgcgc gtcgccaaac gtatggacaa
      841 actcgcccgc ctccaatccg cacacttgcg ccatcatcat ggtcaacagc gcgtagctgg
      901 caatattaaa cggcacacca aggaaaatat ccgcgctgcg ctggtaaagc tggcaggaca
      961 gtttgccgtc ggcaacgtaa aactgaaaca gcgcgtggca gggcggcaat gccatttcat
     1021 cgaccaaagc cggattccac gccgatacaa tcaggcggcg cgagtcgggg tttttcttaa
     1081 tttgttccac cacattggcg atttggtcga tatgcctgcc gtcgggcgcg ggccagttac
     1141 gccactggta gccgtaaacc gggcctaagt cgccgttttc gtccgcccac tcgtcccaaa
     1201 tggaaacatt gttgtccttt aggtatttga tattggtatc gcctttgaga aaccaaagca
     1261 gctcgtggat aatcgagcgc aaatgcagct ttttggtcgt cagcagcgga aaacctttgc
     1321 ccaagtcaaa acgcatctga taaccgaata cggagcgcgt gcccgtaccg gtgcggtctg
     1381 atttgtccgt accgttgtcg aggacgtggc gcatcaagtc caaataggcc ttcatcatca
     1441 tctttcatca aattaaacgg cgcatattgt aacatttccg gccaacgccc gagtccggac
     1501 aaacgcacac gccgccccgc cggacgataa aaacgcagat acaggcaggc aggattgttg
     1561 gcaatttcag tccttttcca cagtaaaacc cagtgggaaa acaaaattac cttgattgga
     1621 atcaaaaaat ctagtttaat tacttagaat aaaatttcaa taatattttt atttacgaaa
     1681 ttaatttatg attattgatt aattatcggc aacaaacatc aaacatcgaa aacatggaaa
     1741 aaataatgtc aacaattttt gccaaatcgg gcttggcatc agaaaaaaat aggtttatat
     1801 tcccacctac aaatttgttt tcccattagt acactatcaa ccaaaaggag tatccgaatg
     1861 actgacctga acaccctgtt tgccaacctc aaacaacgca atcccaatca ggagccgttc
     1921 catcaggcgg ttgaagaagt cttcatgagt ctcgatccgt ttttggcaaa aaatccgaaa
     1981 tacacccagc aaagcctgct ggaacgcatc gtcgaacccg aacgcgtcgt catgttccgc
     2041 gtaacctggc aggacgataa agggcaagtc caagtcaacc ggggctaccg cgtgcaaatg
     2101 agttccgcca tcggtcctta caaaggcggc ttgcgcttcc atccgaccgt cgatttgggc
     2161 gtattgaaat tcctcgcttt tgaacaagtg ttcaaaaacg ccttgaccac cctgcctatg
     2221 ggcggcggca aaggcggttc cgacttcgac cccaaaggca aatccgatgc cgaagtaatg
     2281 cgcttctgcc aagcctttat gaccgaactc taccgccaca tcggcgcgga caccgatgtt
     2341 ccggccggcg acatcggtgt aggcgggcgc gaaatcggct acctgttcgg acaatacaaa
     2401 aaaatccgca acgagttttc ttccgtcctg accggcaaag gtttggaatg gggcggcagc
     2461 ctcatccgtc ccgaagcgac cggctacggc tgcgtctatt tcgcccaagc gatgctgcaa
     2521 acccgcaacg atagttttga aggcaaacgc gtcctgattt ccggttccgg caacgtggcg
     2581 caatacgccg ccgaaaaagc catccaactg ggcgcgaaag tgctgaccgt ttccgactcc
     2641 aacggcttcg tcctcttccc cgacagcggt atgaccgaag cgcaactcgc cgccttgatc
     2701 gaattgaaag aagtccgccg cgaacgcgtt gccacctacg ccaaagagca aggtctgcaa
     2761 tactttgaaa accaaaaacc gtggggcgtc gccgccgaaa tcgccctgcc ctgcgcgacc
     2821 cagaacgaat tggacgaaga agccgccaaa accctgttgg caaacggctg ctacgtcgtt
     2881 gccgaaggtg cgaatatgcc gtcgactttg ggcgcggtcg agcaatttat caaagccggc
     2941 atcctctacg ccccgggaaa agcctccaat gccggcggcg tagcaacttc aggtttggaa
     3001 atgagccaaa acgccatccg cctgtcttgg actcgtgaag aagtcgacca acgcctgttc
     3061 ggcatcatgc aaagcatcca cgaatcctgt ctgaaatacg gcaaagtcgg cgacacagta
     3121 aactacgtca atggtgcgaa cattgccggt ttcgtcaaag ttgccgatgc catgctggcg
     3181 caaggcttct aagcaaacgc cgccgctccg caaacaaaat gccgtccgaa ccgcaaatgc
     3241 tgttcagacg gcatttcatt atccgcccgt tcgaatcggg tgatactatt tgtatccaga
     3301 attgttggat agtttgggag tggagaggaa gaaatgatta tggaccaata ctatatggag
     3361 ttaaagaata agttatctaa cagaccaatc ctactggata atactaatga ttttcttttt
     3421 gttttagtaa atacagtgaa agctatgata gaaaatactg ataaaagtca gctttcagag
     3481 ttagataaaa ttttagacgg tgtcacaagt caagaattga aacttgccta tgatttttgt
     3541 caagggaaat tcggtcaagc aggtttttct tatcgtaggc acccgaatta tttttatttg
     3601 tccagtttaa ttgcgacttt tccagaattt gagttgtcta aagcagaccg agattacctc
     3661 aaaggaatca taaattttga taactacctt ttatatgagt tagattagtc tttaatcacc
     3721 tcaaaaccct atttattcaa aaggagaaaa aatgattgag ctgaaattag tagatgagag
     3781 cagttttcag gcagtgctgg atttgaaaat atctgaagct gatgaacgag cacgttttgt
     3841 ggctccaaat gtacgctctt tagctgacgc atggctctac cgggaaaatg aagatgtgtt
     3901 tccgagggca atctattggg ataagcaagt ggttggcttt ctcctgctgg aaatagacaa
     3961 ggatgaagca gaatacttta tctggcggat aatgattggt cagcagtg
//
LOCUS       DAXHMX010000162         3999 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHMX010000162 DAXHMX010000000
VERSION     DAXHMX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.162"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(64..1314)
                     /gene="glyA"
                     /locus_tag="ACFM4S_001906"
     CDS             complement(64..1314)
                     /gene="glyA"
                     /locus_tag="ACFM4S_001906"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6051529.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     ETQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYTVYGA"
     gene            complement(1469..1732)
                     /locus_tag="ACFM4S_001907"
     CDS             complement(1469..1732)
                     /locus_tag="ACFM4S_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051530.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLPLGSLVLT"
     gene            complement(1795..3570)
                     /gene="ggt"
                     /locus_tag="ACFM4S_001908"
     CDS             complement(1795..3570)
                     /gene="ggt"
                     /locus_tag="ACFM4S_001908"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6051531.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPSKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3738..3999)
                     /locus_tag="ACFM4S_001909"
                     /pseudo
     CDS             complement(3738..3999)
                     /locus_tag="ACFM4S_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin family protein"
BASE COUNT          995 a          952 c          945 g         1107 t
ORIGIN      
        1 gcctgaaaca ttgtgagatt tcaggcggca ttggattgct tggcggaata tttttaaaaa
       61 ggcttacgcg ccgtaaacgg tgtatttatt gcacaaagca gttacttgtt cgcggacttt
      121 ggcgaggttg gcttcgtctt cggggttaga caatacgtcg gcaaccaagt tcgccaatac
      181 gcgcgcgtcg gcttcgttaa aaccgcgtgt ggtcatggca gcggagccga tgcggatgcc
      241 ggaggtaacg aagggttttt ccggatcgtt cgggatggcg tttttgttga cggtgatgtg
      301 cgctttgccc aaagcggctt cggcggcttt accggtgatt ttcatcggtt gcaggtcgac
      361 gaggaaaacg tggctttcgg tgcggccgga aacgatgcgc aaaccgcgtt taaccaactc
      421 ttccgccatg gcggctgcat tgattttcac ttgttttgcg tattgtttga actcgggttg
      481 caatgcttct ttaaacgcca cggctttggc ggcgataacg tgcatcagcg ggccgccttg
      541 caggcttggg aagatggaag agttcaacgc tttttcgtgg gtattgtcgc ggcacaaaat
      601 tacgccgccg cgaggaccgc gcagggtttt gtgggtggtg gtggtaacga agtcgcagaa
      661 tggcaccggg ttgggatatt cgccaccggc aatcagtccg gcgtagtgcg ccatgtcgac
      721 aaagaggtat gcgccgactt tatcggcgat ttcgcggaat tttgcccagt cgatttgcaa
      781 cgcgtaggca gacgcacccg ccacaatcat tttgggtttg tgttcgagcg cgaggcgttc
      841 gacttcggca taatcgagca cttcgttttc atccaaacca taagtaacgg cattgtagag
      901 tttgcctgag atattaacgc tcgcgccgtg ggtcaggtgg ccgccgtgcg ccagagacat
      961 acccaaaatg gtgtcgcctg gttttaaaac ggaagcgtac acggcttggt tggcttgcga
     1021 gccggagtgc ggttggacgt tggcgtactg cgcgccgaac agttttttga cgcggtcaat
     1081 cgccaattgt tcgacaatat cgacgtattc gcagccgccg tagtagcgtt tgccggggta
     1141 gccttcggcg tatttgttgg tcagctggga accttgcgtt tccattacgg cgcagctgac
     1201 gtagttttca gaggcaatca gctcgacgtg gtcttgctgg cgttggtctt cttgggcaat
     1261 ggctgctgcc aaatcggggt catattgtgc gagggtaacg ctttttgaaa acatgttctc
     1321 ggctcctttg tgtaatcagg gtatcatgag tgttttttgt ataaaaaaat acttcaaaac
     1381 ctaaggcaga tagtccataa tgcgtaaatt ttctttggca ttatcaggta atttatttaa
     1441 catgctggtt tttagcgtct cattaccttt atgttagtac aaggctaccc aaaggcaata
     1501 catcaaatgg taaattttcg gcagtttgtg caaaatcaat caaataacca gcaccaacca
     1561 catctttatc agccataacc cgctgataca ccacttgcca agacaactct cctgccccac
     1621 catgtcttaa aatcagatga atggcttgta atgctgtgtt tggcgtctca caaattgcga
     1681 agatctgctc agaagcttgc ttgatggctt gtatttttgt ttgattactc acaatttccc
     1741 cctattttaa tattaactta atgcggtcaa attcacaaaa tacaagcttc acctctaatc
     1801 acccatcact cgaccttctc ggcgtggatc ggcaccacca accagcctgc ttggctcgat
     1861 aataatggct tgaacacctg aatttagctc acgcacgtca gtcttatagc ccaaatcatt
     1921 taatgcttgt tgccactgga gggcggttgt acccgtttct agttcatagc taccaaagcg
     1981 atttaataaa ttgggtgcac tgatggcatc ttggatatcc atattccagt cgctatgtgc
     2041 cacaatcgtc ttagcgacat agccaatgat acggctacca cctggggagc cgattgccat
     2101 ataaggcttg cctgctttaa atacgatggt tggtgccatt gaggagcgtg gtctcttgct
     2161 gggctcgaca cgattggcga cctgtttgcc ctgctttatt ggctcaaaac taaagtctgt
     2221 cagctcatta ttcagcaggt agccatttgc catcaaagtt gagccaaacg cattttcaat
     2281 ggaagtcgtc attgatagca cattgcccgc cttatccaca attgatatat gactggtaga
     2341 aggtaactca atcgcttgtg aggacaccca ctcatgaata aaatcgcctg cagatacgct
     2401 aggcaatgcc ttatccgact gctcaagcag ctggctgcga tgttttaggt agtctttaga
     2461 aatcaactgg cgaatgggta ctggtacaaa atcagggtcg cccaaatata catcacgatc
     2521 cgcaaacgca agcctagaag cgtcgcccaa gagacgtaaa ccttcagcat cataccccac
     2581 ctgattgggt gaaaattcat ttaaaatccc caaaatttga cccacagcaa tcccacctga
     2641 gcttggtgca cccataccgc atacttcata aatacgataa gtcacacaaa caggcgggcg
     2701 ttccaccact tgataatcag ataaatcttg taaggataat tgaccggggt tatccttagc
     2761 attttggaca actgaaacga tattttgggc atatttacca gtatgcagag cttttgcacc
     2821 ttgagctgct aacgcctgaa cactgtcagc aaattctaaa tttttcagca agctgcctgc
     2881 ttgtagcggc acaccattcg gcaaaaaata agcggctgtt tttggatagc gtgccaaatg
     2941 ctgctgattt tgctcaaccg agatggcaag ccttggcgac acctcaaagc cttgttttgc
     3001 caagcggatc ggtgtatcaa ataattttcc ccaaggcaat acaccgtatc gctgatgtat
     3061 tgtctccatc agtttaggga tagcaggcgt acccaccgag cgaccaccga ccaccgcttc
     3121 cataaatttc aatggttgac catctttatc caaaaataat tccggcgtcg cacgcatcgg
     3181 tgccgtctca cgcccatcaa atgtggtcaa tgttttggcg gtattatccc aatacaacac
     3241 aaatgcacca ccgcccaagc ctgacgactg tggctctacc aagcttagtg tcgtctgcac
     3301 cgccaccatc gcatctgcag cgctaccgcc ttgctttaag atatcatagc cagcttgtgt
     3361 tgctaatgga ttggctgacg ctaccataaa atcacttgca atcacctgct tttgttcgat
     3421 cagtcccgtt gcatgttcag gcgtgtgagc gtctgcacct gtgatgacag cagaatgagt
     3481 attaacctta ccttgattgg catggatgac ttgacatcca gagattgtca tagacattat
     3541 caatgcagtc aataaatatg ttttagccac aagcactcct tcgcctgagt ttgattgatg
     3601 attcatacaa ggcatgctga ttattgtatt taatatggct aaataattca atccaaacta
     3661 tcaatcttga ccatcaaaaa aagaccgcta atgtcatcag cagtcttttt tgatatttat
     3721 tttaagatat taagtaatca gacctttggg ctatgctctt caatgagtgg ttttagctca
     3781 cctgattggt acatttgtag gataatatca ctaccaccga ttaactcacc attaacccaa
     3841 agctgtggaa aggttggccg attggcgatg agtggtagag tactgcgaat ttctgggttt
     3901 tctaggatat taacaaaagc aaagggtctg ccaattgggt cagcacctct actgcacgcg
     3961 ctgaaaatcc acattgggga aactggggcg tgcctttca
//
LOCUS       DAXHMX010000163         3862 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHMX010000163 DAXHMX010000000
VERSION     DAXHMX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.163"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="ACFM4S_001910"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="ACFM4S_001910"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6051532.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="ACFM4S_001911"
     CDS             1439..1924
                     /locus_tag="ACFM4S_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6051533.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM4S_001912"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM4S_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6051534.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVGTLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          788 a          937 c         1077 g         1060 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggttcc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 agaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattgc cgattttggc
     3181 atcccaatca ggcaggcgca gcgcgtcggc ggcgatttcc agttcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg cgataatcgc ttccaagcga ccttgttctt ccgccaatgc
     3301 gtcaaaatcc gcatcaggat tggcatactc ggcatacact tcttccaaac gtttttgtgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggttttct caggatcaag
     3421 ctcaggctct tgcggcaaat agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcttta tccacgcccg ccataatccg cagtacagta gatttacccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaagc aa
//
LOCUS       DAXHMX010000164         3857 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHMX010000164 DAXHMX010000000
VERSION     DAXHMX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.164"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..274
                     /locus_tag="ACFM4S_001913"
     CDS             <1..274
                     /locus_tag="ACFM4S_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6051535.1"
                     /translation="NETTKGACKGFCYSHSSYFAEVPEQYQRDASGEYVIRDGKKVET
                     EAWKDYKSIWGEPTEKDENGKYINKYIPKLINPNKPNGEKYEDKPF"
     gene            255..563
                     /locus_tag="ACFM4S_001914"
     CDS             255..563
                     /locus_tag="ACFM4S_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051536.1"
                     /translation="MKINHFNIALLFVSTMMLTGCFGHCADFNCTRLDPRWVKAVDTC
                     ETKNLTIVLQVLGEDLGYKSLIEVQDSKIGQHISSQCIYHSGKYDIHKDKQYEHLFAK
                     "
     gene            911..2836
                     /locus_tag="ACFM4S_001915"
     CDS             911..2836
                     /locus_tag="ACFM4S_001915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051537.1"
                     /translation="MTVGYGFSAGGDYSQSKIRADHASVTEQSGIYAGEDGYQINVQN
                     HTDLKGGLITSTQKSEDDGKNTFSTGTLTFEDLANHAEYKGEAIGLGVSGSVAGDNPN
                     QNSRIYTVNQSGMSSTMGYGKDSDKQYATTHSGINTANITIRDNEKQQQITGISAEEA
                     KRAVKADVTTDNYAEHSGSLKNNFDKDKVQSEIDLQVKVTQEFDSNRKALRAEINKAA
                     DEKRQKAEDIRRENYIDGKNGYNTAESLALDKSADKLEKVAFYTDLALGGLYGIGDST
                     ALTYVTGSAVANPAIRAGTRPRQFWFEKCDGDSLYCSNRNYDDTADRKIYDKNGQEID
                     TKDPNYTGMVEIGDKRQISDLNEIEPNKVTGSIVTTNDGILNPRNDALKNGIKQQKWE
                     VDKEGILLIYNPPTGNSISELLYAAYDKTNDLLGGRLPLASAQKANLELNQYAESKGY
                     KIDSNNHSRGGLTRSVSDKYSVRHNLPLPYNNVQFFGSAANVDDYTNYMNEYGRKDFN
                     ISATTHYTDFVGRTPLVALRSKHIVGGNEPTGGVEDKWFLYSHSSYFAEKPTPYLVNE
                     HGFYMDKNGDLIKDNSGNPIKMTEENKLEYSTKVPNPYLDDFEQKWIKGQTHNKVSGD
                     NPSLPVPVRTKKQQGER"
     gene            2836..3267
                     /locus_tag="ACFM4S_001916"
     CDS             2836..3267
                     /locus_tag="ACFM4S_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051538.1"
                     /translation="MKKYFFSVCLLLTGCGVLFDDPLTRFWNGGYFLFPDKEIASDGI
                     YKTSDTDLHYYESLSLEQRKRYLKFKPIKDKCEHKYSDRTKYDYATQMANIVRCFNEQ
                     GYQSETAFILNGHTITQKGSICTKDEPKQCDNVMRSYIVLF"
     gene            3309..3560
                     /locus_tag="ACFM4S_001917"
     CDS             3309..3560
                     /locus_tag="ACFM4S_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051539.1"
                     /translation="MKPIYFIIFSFLLTACVVNNDDYFAEPPTPYLINEHGFYMNKKG
                     DLIRDSHGNAIKMTEENKHNFPKESANPYYEKWIKQKRK"
BASE COUNT         1416 a          659 c          782 g         1000 t
ORIGIN      
        1 taatgagaca acaaaaggag cttgtaaggg attttgctat tcgcatagca gttattttgc
       61 ggaagtgcct gaacagtacc aaagggatgc gagcggagag tatgttatta gagatggtaa
      121 aaaagttgag acagaagctt ggaaagatta taagtcaata tggggcgaac cgaccgaaaa
      181 agatgaaaat ggtaaataca tcaacaaata tattcctaaa ttaatcaacc caaataaacc
      241 aaatggagaa aaatatgaag ataaaccatt ttaatatagc attacttttt gtttccacca
      301 tgatgctaac aggatgtttt ggtcattgtg cggattttaa ttgcacgaga ctcgatccac
      361 gctgggtaaa agcagttgat acatgtgaaa ctaaaaatct cacaatagtg ttacaagtat
      421 taggggaaga tttaggatac aaatctctca ttgaggtaca agacagtaaa attggtcaac
      481 acatttcttc tcaatgtatt taccatagcg gtaagtatga tattcataaa gataagcagt
      541 atgagcattt gtttgcaaaa tgatattacg ccaatgtttt acagaaaaac ggctatacct
      601 atacgggtgc ggacggcaaa acttataatt cgattgtgca taataaggat tttgtggaga
      661 acaaagggat acctttcttg ctagaaacca atgacaccac acaaggtata tgtaagggat
      721 tttgctatcc gcatagcaat tattttacgg aagtgcctga acagcaccaa aaggataaga
      781 atgggaatat tgttaaaaag aatggtgaac ctgcatcaaa ggcgtacaag tcaatgccaa
      841 aaacctaagt attcaaagcg tacaagatag agaaacttat caaagcaaac aacaaaacgc
      901 cagtgcacaa gtgaccgtag gctacggctt cagtgccggt ggcgattaca gccaaagcaa
      961 aatccgagcc gaccatgctt cggtaaccga gcaaagcggt atttatgccg gagaagacgg
     1021 ttatcaaatc aatgtgcaga accacaccga tttgaaaggc ggtttgatta cttccaccca
     1081 aaaatcggaa gacgatggca aaaacacatt ctcaacgggt acgctcacct ttgaagacct
     1141 agcgaatcac gccgaatata agggtgaagc gattggtttg ggtgtttcag gcagcgttgc
     1201 gggcgataat ccaaatcaaa acagccgtat ttataccgtt aatcaaagcg gtatgagtag
     1261 cacaatgggc tatggcaaag acagcgacaa acaatacgcc acaacccatt caggcattaa
     1321 cactgccaat atcaccattc gtgataacga aaaacaacag caaatcacag gaatatcggc
     1381 ggaagaagcg aaacgggctg ttaaagccga tgtaacaacc gacaactacg ccgaacattc
     1441 aggcagcctg aaaaacaatt tcgataaaga caaagtgcaa tcagagattg atttgcaggt
     1501 taaggttaca caggagtttg atagtaatcg caaagccttg cgtgcagaaa tcaacaaagc
     1561 agcagatgaa aaaagacaaa aagccgaaga tataagaaga gaaaattata ttgacggcaa
     1621 aaacggttat aacaccgcag aatctttggc tttggataag tctgccgaca aattggaaaa
     1681 agtcgctttt tacactgatt tggcattagg tggattgtat ggaattggcg atagcacggc
     1741 tttaacttat gtaacaggta gtgcagtggc taatccagcc attcgagcgg gtacaagacc
     1801 aagacagttt tggtttgaga aatgtgacgg cgatagtttg tattgttcta atagaaatta
     1861 tgacgatacg gcggatagaa aaatttatga taaaaacggg caagaaattg acactaaaga
     1921 tccaaattac actggaatgg ttgagattgg tgataaacgg caaatctctg atttaaatga
     1981 gattgaacct aacaaagtaa ctggaagcat cgttactaca aatgacggta tattgaaccc
     2041 acgaaatgac gctctcaaaa atgggattaa gcaacaaaaa tgggaagtag ataaggaagg
     2101 tattttgctt atctacaatc cgccaacagg caattctatt tctgaacttt tgtatgccgc
     2161 ttatgataaa actaacgatt tgttaggcgg tcgtttgcct ttagcttctg cacaaaaagc
     2221 gaaccttgaa ttaaatcaat atgcagaaag taaaggttat aagattgata gtaacaatca
     2281 tagtcgtggc ggtttaactc gaagtgtttc tgataaatat tcagtcagac ataatctgcc
     2341 tttaccgtat aacaatgttc aattcttcgg tagtgcggca aatgtagatg attacacaaa
     2401 ctacatgaac gaatatggta ggaaagattt taatatttct gccacaacac attacacaga
     2461 ttttgttggt agaactccat tagttgcatt gcgtagtaag catattgttg gtggaaatga
     2521 accaacaggc ggagttgaag ataaatggtt cttatattcg cacagcagtt attttgcaga
     2581 aaaaccaacc ccataccttg taaatgaaca cggtttttat atggataaaa atggcgacct
     2641 gataaaagat aattcaggaa atcctataaa aatgacggaa gaaaataaat tggaatattc
     2701 aacaaaagtt ccaaatccat atttagatga ttttgaacag aaatggataa aaggacaaac
     2761 tcataacaag gtgtcaggtg ataatccgtc tcttccagtt ccagttagaa caaaaaaaca
     2821 acagggagag cgataatgaa aaaatacttc ttttcagtct gcttgttatt aacaggctgt
     2881 ggggttttgt ttgatgaccc actaacaaga ttttggaatg gcggttattt tttgtttcca
     2941 gacaaagaaa tagcaagtga tggaatttat aaaacttcgg atactgactt gcattattac
     3001 gaaagtttgt cgcttgaaca aagaaaacgc tatctaaaat ttaagcctat taaggataaa
     3061 tgcgaacata agtattcaga tagaacaaaa tatgattacg ctacacaaat ggcaaatatc
     3121 gttaggtgtt ttaatgaaca aggctatcaa tctgaaactg cttttatatt gaatggacat
     3181 acaataactc aaaaaggtag tatttgcaca aaagatgaac ccaaacaatg cgataatgtt
     3241 atgcgtagtt atatcgtttt attttaattg aaatgcaact tgaaaattga caatacagga
     3301 gcaacaaaat gaaaccgatt tattttatta ttttttcttt tttattaact gcttgcgtgg
     3361 ttaataatga tgattatttt gctgaaccgc ctacaccata cttaattaac gaacacggtt
     3421 tttatatgaa taaaaaaggt gatttaatta gagatagtca cggtaacgca attaaaatga
     3481 cagaagaaaa taaacacaat ttccccaaag aatccgctaa tccttattac gaaaaatgga
     3541 taaaacaaaa acgaaaataa ataacaaagc aaacatagtg taggcaactt gttgcccacg
     3601 cgttaatgat aactttcagg cagcctgaaa gcctaaaacc acaaggaaag ctccaaaaat
     3661 gaacaaccgc ttacacaaag tcatcttcaa ccgcaacaca gcaagcgtag ggtgggcaac
     3721 ttgttgccca cgcagttcaa tcagatacac ataaaatgat gttttaatgt gaaatatttg
     3781 atttcaggct gtctgaaaaa taaaatgatg tggtgtgcat caaaccgcgt gggcaacaag
     3841 ttgcccaccc tacgctt
//
LOCUS       DAXHMX010000165         3848 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHMX010000165 DAXHMX010000000
VERSION     DAXHMX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.165"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..2076
                     /locus_tag="ACFM4S_001918"
     CDS             <1..2076
                     /locus_tag="ACFM4S_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC6051540.1"
                     /translation="GKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFGG
                     ESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMNPE
                     GAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKAFGVRANGKLRH
                     GDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLFG
                     APDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVCL
                     NATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLSARGEFETGPVTHN
                     WSTAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFTADYPNSWENSANLDANI
                     KVNSLALSDTLGFVDNKYRLTLGERFQTVEYTDKKSQSGDAKRFSPMLMAAWVPQPDL
                     VVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGDFVTTLNAFQIKRPG
                     YWRGNTKKGTDFAAYKAAGGADGDEQGMERSRGIEFNAYANLLNKTLRPTFGLMYLQS
                     TVKDYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNSNVSYFGKSYQDTQKQ
                     YAFPSYTLVDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQRGQYDRSFAVVGLPRT
                     YWLKAELDF"
     gene            2200..3750
                     /locus_tag="ACFM4S_001919"
     CDS             2200..3750
                     /locus_tag="ACFM4S_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6051541.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNAQKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         1087 a          951 c          969 g          841 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gagaaagcaa cacgctgact ggactgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttggca ggtatgtacg gcacgcaagg cacggccagc
      241 gtgcaagtcg gctccgcaca actgattaaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgaaggtg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgatttg
      421 ggtcaacgct tcggcgaaaa caaagcattc ggcgtacgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtt tggcgcgccc
      661 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      721 atgcttacgt ttgaatggga tgcgtttgat aatgcacaga ttacaggcgg tatcggctac
      781 aacaaggcac gctattacgg cacgctgatt tccccgaccg tatgtttgaa tgctaccagc
      841 acttgtaccg atatgcaaaa cacaaacgcc agcggcaaag taacggcacg caactatgac
      901 tacaataccg gtactgcgcg cctgaccgac caatacttcc gcactttaag catgaacctg
      961 tctgcgcgag gcgaatttga aaccggcccg gtaacacata actggagtac ggctttcgac
     1021 cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag caagatagaa
     1081 gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt ttacagccga ctatcccaat
     1141 tcttgggaga actctgccaa cttggatgcc aatatcaaag tcaacagcct ggctttgtcc
     1201 gacaccttgg gcttcgtgga taacaaatac cgcctgactt tgggcgaacg tttccaaaca
     1261 gtcgaatata ccgacaaaaa atcacagagc ggcgatgcca aacgtttcag cccgatgctg
     1321 atggcggcgt gggtgccgca acctgatttg gttgtgtacg gcaactatat ggaagacttg
     1381 gagcctgccg acattaaaac cgatgattcc ggtgaaacga caatggcgaa accgcgcgtc
     1441 agccgccagt ttgaagtggg cgttcgtaaa aactggggcg actttgttac gacgttgaac
     1501 gcgtttcaaa tcaaacgccc gggctactgg cgcggcaata ccaaaaaagg tactgatttt
     1561 gcagcatata aagcagcagg aggcgcagac ggagatgaac aaggcatgga acgcagccgc
     1621 ggtatcgaat tcaatgccta tgccaacctg ttaaacaaaa ccctgcgccc gactttcggc
     1681 ctgatgtacc tgcaatcgac cgtgaaagac tacccaaatt cacgcgatat gctggttaac
     1741 ggcgtacaag tcgccaatcc gcgcgtaatt gccaaggcag gtttggaatg ggatacaccg
     1801 tttgccaaag gcttgacttt aaacagcaat gtttcgtatt tcggcaagtc ttaccaagac
     1861 acgcaaaaac aatacgcctt cccatcctat accttggttg atgtaggcgc gcgctacaaa
     1921 accaagctcg gcaaaaatac gttgaccgtc agcagctcgg tggaaaatct gttcaacaaa
     1981 aactactggc aggtacagcg cggccaatac gaccgcagct tcgccgtcgt cggtctgccg
     2041 cgtacttact ggctgaaggc ggaattggat ttctgatttc tccgcatctg gaaaatgccg
     2101 tccgaacagg tttcagacgg catttctttt aaagagatat ggcgggatac gcttaaataa
     2161 tgctattcta gcgtctttga aacctgcttg tttgaagtga tgaaaaaacc tttgatttcg
     2221 gttgcggcag cattgctcgg cgttgctttg ggcacgcctt attatttggg tgtcaaagcc
     2281 gaagaaagct tgacgcagca gcaaaaaata ttgcaggaaa cgggcttctt gaccgtcgaa
     2341 tcgcaccaat atgagcgcgg ctggtttacc tctatggaaa cgacggtcat ccgtctgaaa
     2401 cccgagttgc tgaataatgc ccaaaaatac ctgccggata acctgaaaac agtgttggaa
     2461 cagccggtta cgctggttaa ccatatcacg cacggccctt ttgccggcgg atttggcacg
     2521 caggcgcaca ttgaaaccga gttcaaatac gcgcctgaaa cggaaaaagt tttggaacgc
     2581 ttttttggga aacaagctcc ggtttccctt gccaataccg tttattttaa cggcagcggt
     2641 aaaatggaag tcagtgttcc cgccttcgat tatgaagagc tgtcgggcat caggctgcat
     2701 tgggaaggat tgacgggaaa aacggtttat caaaaaggtt tcaaaagcta ccgcaacagc
     2761 tatgatgcgc ccttgttcaa aatcaagctg gcagacaaag gcgatgccgc gtttgaaaaa
     2821 gcgcatttcg attcggaaac ttcagacggc atcaatccgc ttgctttggg cagcagcaat
     2881 ctgaccttgg aaaaattctc cctagaatgg aaagagggtg tcgattacaa cgtcaagtta
     2941 aacgaactgg tcaatcttgt taccgatttg cagattggcg cgtttatcaa tcccaacggc
     3001 agcatcgcac cttccaaaat cgaagtcggc aaactggctt tttcaaccaa gaccggggaa
     3061 tcaggctcat ttatcaacag tgaagggcag ttccgttttg acacgctggt gtacggcaat
     3121 gaaaaatacg gcccgctgga catccatatc gccgccgaac atctcgatgc ttctgcctta
     3181 accgtattga aacgtaagtt tgcacaaatt tccgccaaaa aaatgaccga ggaacaaatc
     3241 cgcaatgatt tgattgccgc cgtcaaaggc gaggcttccg gactgttcac caacaatccc
     3301 gtattggaca ttaaaacttt tcgtttcaca cagccgtcgg gaaaaatcga tgtgggtgga
     3361 aaaatcatgt ttaaagacat gaagaaggaa gatttgaacc aattgggttt gatgctgaag
     3421 aaaaccgaag ccgacatcag aatgagtatt ccccaaaaaa tgctggaaga cttggcggtc
     3481 agtcaggcag gcaatatttt cagcgtcaat gccgaagatg aggcggaagg cagggcaagt
     3541 cttgacgaca tcaacgagac cttgcgcctg atggtggaca gtacggttca gagtatggca
     3601 agggaaaaat atctgacttt gaacggcgac cagattgata ctgccatttc tctgaaaaac
     3661 aatcagttga aattgaacgg taaaacgttg caaaacgaac cggagccgga ttttgatgaa
     3721 ggcggtatgg tttcagagcc gcagcagtaa tgggtcaaat gccgtctgaa atgttcaggc
     3781 ggcattttgt tgtattggaa actgccgttt tcaaccattc ctttagataa gccgtgtcgg
     3841 attcgttt
//
LOCUS       DAXHMX010000166         3758 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHMX010000166 DAXHMX010000000
VERSION     DAXHMX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.166"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..294)
                     /locus_tag="ACFM4S_001920"
     CDS             complement(<1..294)
                     /locus_tag="ACFM4S_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFC6051542.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALASVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVS"
     gene            complement(641..943)
                     /locus_tag="ACFM4S_001921"
     CDS             complement(641..943)
                     /locus_tag="ACFM4S_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051543.1"
                     /translation="MGYRVGMNCFDTRLQADDYLLSSLPPTVTEEGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIFGFRLLINFLKDMGKVGTD"
     gene            complement(971..1201)
                     /locus_tag="ACFM4S_001922"
     CDS             complement(971..1201)
                     /locus_tag="ACFM4S_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051544.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDAAAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(1439..1639)
                     /locus_tag="ACFM4S_001923"
     CDS             complement(1439..1639)
                     /locus_tag="ACFM4S_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051545.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            complement(1643..1954)
                     /locus_tag="ACFM4S_001924"
     CDS             complement(1643..1954)
                     /locus_tag="ACFM4S_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051546.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(1966..3180)
                     /locus_tag="ACFM4S_001925"
     CDS             complement(1966..3180)
                     /locus_tag="ACFM4S_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6051547.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKT
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
BASE COUNT         1190 a          876 c          560 g         1132 t
ORIGIN      
        1 agatacaacc gaactaactt tacccgtact tgatgctcca gtagatacat gcctaacttt
       61 taaatcttga gcttctgaag ttaaatatct tcccgattct ctatcaaata cacctttcca
      121 aattcttctt cttttgtcat ctgaacctcg aatacctaaa tatctgccat catcataaac
      181 ttcaacccta tcaaatttac ccggaacatt tactgatgcc aatgcaaatt taggtacaaa
      241 aaaactaaag cataggataa tctttaaaaa atttcgccct aggataagaa acataacatt
      301 ttgcttcatc taatttaatt aataatgtag aagaatcagg gaattgaatt tctataaaat
      361 ctctatcata acgatgaaag aaattcctaa aaatatcaaa tgaaaacgaa gttttaaaaa
      421 ccccttttct aatcaattcc ctataataaa ttaacgcttg tggtaaatct ctataaatat
      481 gtgaatcagt ttgaaattta actaaatgat attcagaaat aaacataatt cggacctttt
      541 aaaacactct aaaaagccga ttctaccaac cgttaccaaa acagccaagc aacagacaaa
      601 gccaagaaga aaccgagaag aaaccaaaaa tcaataatca tcaatcagta ccaaccttgc
      661 ccatgtcttt taagaaatta atcagaagcc tgaaaccgaa aatgactacg aacagaatta
      721 gaaccgtcga accgacataa gaaccttgct ttatctgctc aaaattggaa cattccggat
      781 aagacaacgt aaccggcttt ccgttcaaaa tccatttatc gcccaccctt tccggcctga
      841 tgatttttcc ttcttctgta acagtaggag gaagagacga caataaatag tcgtctgcct
      901 gcaatcttgt atcaaaacag ttcatgccga cacgataacc cattcacgcg cccctctttc
      961 ttcactgccg ttatttgaca gatttaatca tgctccaagc cattttgaag ccttggattg
     1021 ccaaaatgac ggtaatggcc gccataccca cggcggaaac catcgcaacg aaacccatga
     1081 ttacattcgc cacttgcgta ccaatcgcgg ctgcatcaaa gccatctgcc ataacaatag
     1141 ccggtgtgaa gataccagct gccaacaatg cttttacagc atatttttta acgatgttca
     1201 tcgttttttt cctttttttg atatttaaaa taagacgact tcttgacttg cttcatccgg
     1261 acgaagtctt ttccgaatct cgtttttagc cgataaaata gaggattgcg aaaagaaaaa
     1321 gaaacataca gaccacccag ccgataatta gtgttgcaag gttcattttc atgatatttt
     1381 tcctttgttg cgggctttgt gaaaggttaa cagaccgccc gccgagcctg tttttctttt
     1441 attccgattt tacgaagaac tgaaatatct ggaatcctcc gcccatttca tttatgcctg
     1501 aattcaacgc atcttcgtaa ctttcaaatt gacctgctga tttaatattt tgagtaaacc
     1561 ccacatcacc gaacggatcg ggataaataa agtcatgcgt ttctaaatct tgaactatga
     1621 aacgttcttc aaatttcata aatcaacctt tcggcttttc cgccacctga aaatcaatta
     1681 atgacggaac catgccttta cctgtcgaag tcatttcaac cattaccata acttcgcacg
     1741 gatatttgag attctctaac cttgagaaat tcttactatc cccgaacttc atttgtgctg
     1801 ccgtaaatcc aacagcatta cccgactgtg ccggtaaagg tgttgcaacc aatacggaac
     1861 aagtgtcaat gttagagcca tcaatttcac ctttgaattt tttggctccc aaaaaagttg
     1921 caggataagt tacagtttga gtttgattaa acattttgat ttcctttaaa aataatcata
     1981 atctacattt tgatgataaa ctttacttaa atattcctga tatttccttt ctttctcgac
     2041 atcataatca ggactaaaaa gccttttttc cctatcgaat ttgtcagaat ttttaaaagc
     2101 aataaccccc aattcatcaa gttcaatttc caaatcaata tccggctgtt catgaataaa
     2161 accgtgtttc aaaccgtccc ttaacatttc cagagcatat ttttcaggtt ctaatccttt
     2221 gggaaatccc gaatctgcct ttaaagattc cacaatttcg ctattatcaa aacccatttc
     2281 aatcatgaaa ttgaccagtt ttccaaccgc gtttttcgcg taatgcaatt tatgctcgaa
     2341 agttaaatta agcgttttct ttctctgatc aaacctttcg ggaaccggca tatttttaaa
     2401 ttttctacaa attggaaaag ctccacagaa atacgaaccc tgatttatta aaatatccaa
     2461 gggtatttct atatctccat aattaaactg gatctcgaac cttacccatt tgctttcttt
     2521 atctccaagc tgcctgcctt tctcataaac acgaacaaaa cgagaatttt tcttgcgacc
     2581 tacataaaat gtcttgccgc tcccgtcctc attccgccaa gccgtaccga tcgtttcaga
     2641 tttcggcctt tgattgctgt tatcaaaaaa accattatcg tgatctaaca acgcctgatc
     2701 cggcgtgtac tctccatcaa aaaaatcaag tgctaggtca attcgcgtta ttcttgtcct
     2761 tatcgaatca tcgagaaact gctttagcct caactcccaa cccggacttg caacgctgca
     2821 accagtacct ttcaactcaa ctaaaacagt attgcgctga cctccgaaat gcacctctcc
     2881 ataatcaaca tcatccgaac ctaacctata catggattca tagaatttgt tgccccttga
     2941 tttgcatttg cgcgttatgc caaaacctag aatttcttcc agctttctgc ttaatacata
     3001 catgtattca gcatcagaaa ataaagggca accggaaact ttcagtaaag tatcttcatg
     3061 gaatgtgaat gaaatccaat caacaaaaac cccgtcttgc ttacctcttc gctgcggaat
     3121 ttctaaaagc ttcccttttc cgtccgatat aaagtgtgag aaatattcaa cttcactcat
     3181 tcgttcacta ctcttggaat tggtttttaa tttcgctccc cccctgttag tcaagggggg
     3241 gctttcagcc gtttcccgtc ttccgcgcta aagcgcgtac gacggtcaac gaccgaaagc
     3301 caaatcctga caaactttta aagatcaaca aaaaagacca caaccatccg ttgtgataat
     3361 cactggaaaa ttcgaaccaa ccgaatctat ataatcgaac gcctgataaa gctttgaaaa
     3421 attctcttga ttaatgagtt tatgcggttc gccatgtctg aactgataaa aacacaaaac
     3481 acaagaatct gattttttaa atattctcca ataagaacag gaaaatatta catttactgc
     3541 tgacataaaa aagccccttt cacttggctg tcaaagggga atgttaagaa aattaatgcg
     3601 cccctttgat agagcgcatc atataaggcg ggaatctaga ccgttcggtt tcggtttttt
     3661 tggttagtgc cgcaacatta aatttctaga ttcccacttt cgtgggaatg acggcggagc
     3721 ggtttctgct ttttccaata aatgacccca acctaaaa
//
LOCUS       DAXHMX010000167         3722 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHMX010000167 DAXHMX010000000
VERSION     DAXHMX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.167"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            275..3478
                     /gene="nalP"
                     /locus_tag="ACFM4S_001926"
     CDS             275..3478
                     /gene="nalP"
                     /locus_tag="ACFM4S_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HFC6051548.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHT
                     GDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGDGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     GTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1010 a         1017 c          990 g          705 t
ORIGIN      
        1 atccactata actagggaaa ttcaaattaa gttagaatta tccctatgag aaaaagccgt
       61 ctaagccggt ataaacaaaa taaactcatt gagctatttg tcgaaagttc aaatttccat
      121 tttaaaacaa ttagtaaaat cgagtttatc ctagttgtcc aagacaaccc ctataataat
      181 ataattcaaa atataaaaac gggttacatc taaacattac ggaattttta ttccctcgcc
      241 tgaattctat tgtcagattc aaggagacct catcatgcga acgaccccaa ccttccctac
      301 aaaaactttc aaaccggctg ccatggcgtt agctgttgca acaacacttt ctgcctgctt
      361 aggcggcggc ggcggcactt ctgcgcccga cttcaatgca ggcggcaccg gtatcggcag
      421 caacagcaga gcaacaacag cgaaatcagc agcagtatct tacgccggta tcaagaacga
      481 aatgtgcaaa gacagaagca tgctctgtgc cggtcgggat gacgttgcgg ttacagacag
      541 ggatgccaaa atcaatgccc cccccccgaa tctgcatacc ggagacttta caaacccaaa
      601 tgacgcatac aagaatttga tcaacctcaa acctgcaatt gaagcaggct atacaggacg
      661 cggggtagag gtaggtatcg tcgacacagg cgaatccgtc ggcagcatat cctttcccga
      721 actgtatggc agaaaagaac acggctataa cgaaaattac aaaaactata cggcgtatat
      781 gcggaaggaa gcgcctgaag acggagacgg taaagacatt gaagcttctt tcgacgatga
      841 ggccgttata gagactgaag caaagccgac ggatatccgc cacgtaaaag aaatcggaca
      901 catcgatgtg gtctcccata ttattggcgg gcgttccgtg gacggcagac ctgcaggcgg
      961 tattgcgccc gatgcgacgc tacacataat gaatacgcat gatggaacca agaacgaaat
     1021 aatgtctgca gccatccgca atgcatgggt caagctgggc gaacgtggcg tgcgcatcgt
     1081 caataacagt tttggaacaa catcgagggc aggcactgcc gaccatttcc aaatagccaa
     1141 ttcggaggag cagtaccgcc aagcgttgct cgcctattcc ggcggtgata aaacagacga
     1201 gggtatccgc ctgatgcaac agagcgatta cggcaacttg tcctaccaca tccgtaataa
     1261 aaacatgctt ttcatttttt cggcaagcaa tgacgcacaa gctcagccca acacactgac
     1321 cctattgcca ttttatgaaa aagatgctca aaaaggcatt atcacagtcg caggcgtaga
     1381 ccgcagtgga gaaaagttca atggctccaa ccattgcgga attactgcca tgtggtgcct
     1441 atcggcaccc tatgaagcaa gcgtccgttt cacccgtaca aacccgattc aaattgccgg
     1501 aacatccttt tccgcaccca tcgtaaccgg cacggcggct ctgctgctgc agaaataccc
     1561 gtggatgagc aacgacaacc tgcgtaccac gctgctgaca acggctcagg acatcggtgc
     1621 agtcggcgtg gacagcaagt tcggctgggg actgctggat gcgggtaagg ccatgaacgg
     1681 acccgcgtcc tttccgttcg gcgactttac cgccgatacg aaaggtacat ccgatattgc
     1741 ctactccttc cgtaacgaca tttcaggcac gggcggcctg atcaaaaaag gcggcagcca
     1801 actgcaactg cacggcaaca acacctatac gggcaaaacc attatcgaag gcggttcgct
     1861 ggtgttgtac ggcaacaaca aatcggatat gcgcgtcgaa accaaaggtg cgctgattta
     1921 taacggggcg gcatccggcg gtagcctgaa cagcgacggc attgtctatc tggcagatac
     1981 cgaccgatcc ggcgcaaacg aaaccgtgca catcaaaggc gatctgcagc tgggcggcga
     2041 aggtacgctg tacacacgtt tgggcaaact gctgaaagtg gacggtacgg cgatgaccgg
     2101 cggcaagctg tacatgtcgg cacgcggcaa aggggcaggc tatctcaacc gtaccggaca
     2161 acgtgttccc ttcctgagtg ccgccaaaat cgggcgggat tattctttct tcacaaacat
     2221 cgaaaccgac ggtggtctgc tggcttccct cgacagcgtc gaaaaaacag cgggcagtga
     2281 aggcgacacg ctgtcctatt atgtccgtcg cggcaatgcg gcacggactg cttcggcagc
     2341 ggcacattcc gcgcccgccg gtctgaaaca cgccgtagaa cagggcggca gcaatctgga
     2401 aaacctgatg gtcgaactgg atgcctccga atcatccgca acacccgaga cggttgaaac
     2461 tgcggccgcc gaccgcacag atatgccggg catccgcccc tacggcgcaa ctttccgcgc
     2521 agcggcagcc gtacagcatg cgaatgccgc cgacggtgta cgcatcttca acagtctcgc
     2581 cgctaccgtc tatgccgaca gtaccgccgc ccatgccgat atgcagggac gccgcctgaa
     2641 agccgtatcg gacgggttgg accacaacgg cacgggtctg cgcgtcatcg cgcaaaccca
     2701 acaggacggt ggaacgtggg aacagggcgg tgttgaaggc aaaatgcgcg gcagtaccca
     2761 aaccgtcggc attgccgcga aaaccggcga aaatacgaca gcagccgcca cactgggcat
     2821 gggacgcagc acatggagcg aaaacagtgc aaatgcaaaa accgacagca ttagtctgtt
     2881 tgcaggcata cggcacgatg cgggcgatat cggctatctc aaaggcctgt tctcctacgg
     2941 acgctacaaa aacagcatca gccgcagcac cggtgcggac gaacatgcgg aaggcagcgt
     3001 caacggcacg ctgatgcagc tgggcgcact gggcggtgtc aacgttccgt ttgccgcaac
     3061 gggagatttg acggtcgaag gcggtctgcg ctacgacctg ctcaaacagg atgcattcgc
     3121 cgaaaaaggc agtgctttgg gctggagcgg caacagcctc actgaaggca cactggtcgg
     3181 actcgcgggt ctgaagctgt cgcaaccctt gagcgataaa gccgtcctgt ttgcaacggc
     3241 gggcgtggaa cgcgacctga acggacgcga ctacacggta acgggcggct ttaccggcgc
     3301 gactgcagca accggcaaga cgggggcacg caatatgccg cacacccgcc tggttgccgg
     3361 tctgggcgcg gatgtcgaat tcggcaacgg ctggaacggc ttggcacgtt acagctacgc
     3421 cggttccaaa cagtacggca accacagcgg acgagtcggc gtaggctacc ggttctgacg
     3481 gacaggaagc agacagccgc aaagatcacg gtctttgcgg ctgtttctta tgaaaagaaa
     3541 accctattcc aattgcctgc ttctattgtt tcaagacttc ttccaaagat tcggcatcaa
     3601 tcagatgtat agcggattaa caaaaatcag gacaaggcgg cgaagccgca gacagtacaa
     3661 atagtacgga accgattcac tcggtgcttg agcaccttag agaatcgttt ctttgagcta
     3721 ag
//
LOCUS       DAXHMX010000168         3680 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHMX010000168 DAXHMX010000000
VERSION     DAXHMX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.168"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..448)
                     /gene="mafA"
                     /locus_tag="ACFM4S_001927"
     CDS             complement(<1..448)
                     /gene="mafA"
                     /locus_tag="ACFM4S_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6051549.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNG"
     gene            complement(567..1667)
                     /locus_tag="ACFM4S_001928"
     CDS             complement(567..1667)
                     /locus_tag="ACFM4S_001928"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6051550.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPGR
                     LRRKLLDLQDTGADELHRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHSYSVQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGEKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLADCFGTRVSLRSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1992..3566
                     /locus_tag="ACFM4S_001929"
     CDS             1992..3566
                     /locus_tag="ACFM4S_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6051551.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          737 a          901 c         1116 g          926 t
ORIGIN      
        1 tgccgttgac ggacagtccg gcggagcgtt cgcctttgcg tccgctgttt ttcgtcaggg
       61 cggcggcggg ggcgttcaaa agcgatgtgg aagtggttac gctggagaga gcgtcggatt
      121 tggtggtggc ggtagtgtcg taggcggggt agctgtattg ggtggcactt tcggggttgt
      181 tgtggtagcc gccgcgtatc agtgcgtcga tagagtagcg tccgccgctt atgttgcccg
      241 aaccttggtc gcccataacg gagacgtaaa gggcggcttt gcgtcctttc agggcggaca
      301 agtccatttc tttgacggcg gcgcgggacg atgcggcgac gagttcttgt tcgacggcaa
      361 agcgtttgcc gccgccgtgg gcgggtatgc cggtcagtgt gccgcaggcg gttaggacga
      421 gggggatgag gaggagcagg gttttcatgg cagggtttgt tttgttttga acgagtttga
      481 gtgtaaatgg attttaaggg tttgtaaacg aaagaggcaa aaatgccgtc tgagcggcgg
      541 aaatggcttt cagacggtat ttgcgctcaa taataatatc ccgcgcccag aatacacggt
      601 ttggatgcgc cggttgcttt gtgcggacta ccgggaatgc gattaatcca acacgccgcc
      661 aaccacgcaa atgcggctgc ttccacccat tgcggatcga gcttcagttc ggcggtgctg
      721 cgtagggaaa cgcgtgtgcc gaaacagtct gccaaatccg ccattaaaac aggattgcgg
      781 atgccgccgc cgcaaatgta catttgacgg gcatctgccg ctgcgtgtga gacggcgtcg
      841 aaaacggttt gcgcggtaaa acgggaaagc gtccgtaata cgtcgtatcg gttttcgccg
      901 ccgtcaaggt aggtttcgag ccaatttagg gcaaacagtt cgcgccccgt gcttttgggg
      961 tggggtcgtg cgaaatacgg gtgggcgagc agcctgtcga gcagttgcgg caatatgttg
     1021 ccttgtgccg ctttttcacc gtttttgtcg taaggcaact gccagtgtgc ctgcgtccac
     1081 gcgtccatca gcatattgcc cggccctgtg tcgaagccga aggcgggtgc gtcggagggg
     1141 agtacgctga tgttggcaat cccgccgatg ttcagtacca cgcgtgtttc tttgtcatcg
     1201 cggaacaggg ctttgtgaaa ggcggggacg agcggcgcac cttgtccgcc ggccgcaagg
     1261 tcgcggctgc ggaagtcgcc gacggtaaaa atctgagtcc gttccgccag cagcggcaaa
     1321 tcggcaagct gtacgctgta actgtgttcc ggcgcgtgtc tgacggtttg cccgtggcag
     1381 ccgagggcgg taatgtcgga cggcgcgagg ttttgactgc acagcagttc ggcggcggtt
     1441 tgcgcgtaca ggcggctgag ttcttgcgac aacatcctgc tgcggtgcag ttcgtccgcg
     1501 cctgtgtcct gcaaatccag caatttgcgg cgtaacctgc cggggtaggg ggtaaaggcg
     1561 tgcccttccg cgcccagcca tttgccgccg tccatccgta ccagcacggc atccgcccca
     1621 tccatactgg ttcccgacat gatgccgatg taaagctgtg tttccatcat cactcccaaa
     1681 ctggtgcaaa acgccatttt aacgtgtatt gacgctcgta tactgatttg ccgccgcagt
     1741 gtaaataaag tgtaaataaa tgtttcaaga cggatggaaa aatattataa tgcgcccgca
     1801 acatccagta gtagaagtgt catacaaacc gtttccggca gcagttttgc attcggtcag
     1861 gtttgggggt attcggatgc ggttcagaag gaaggatgcg tctgccatat cccgaaacgg
     1921 cagttcgacc ggaggcagca gtacagtgtc ggcaacactc atgatttcca ccacattaaa
     1981 ggaagattgc catggctcaa atccaaatga gcgcaaatgt taaaaccatc aacgccgtct
     2041 ttgccgccat gctggtaggt acagtcggct attttattta ttggggcttg ggttataccc
     2101 attacaatta cgccgcctta ttcattattg ccacgatgtt cggcgtgttt atggcgttca
     2161 acatcggcgg caacgatgtt gccaattctt tcggcaccag cgtcggtgcg ggtacgctga
     2221 ccatcccgca ggctttgctg attgcggcgg tatttgaggt cagcggcgcg gtcatcgcgg
     2281 gcggtgaggt aaccaatacc atacgcaaag gcatcgtcga tttgaagggt gttgatttcg
     2341 aacccataca gtttgtgttt attatgatgt ccgcgctttt ggcggcggcg ttgtggctgt
     2401 tgtttgcctc gaaaaaaggg cttccggtat ctaccaccca ttccattatc ggcggcattg
     2461 tcggcagcgc ggtatgtatg gcggtaatga acgatgccgc atcgggcgat ttgatacgtt
     2521 ggggcaagct gggcggtatt ggtgtttctt gggtattgtc gcccgtgttg ggcggcgcgg
     2581 tgtcctattt cctgttttcg cgcgtcaaga aaaacgtctt agattacaac gcttgggcgg
     2641 aaggcacgct caagggcatc aagcaggaaa aaaaggccta taaagaacgg caccgcctgt
     2701 ttttcgaggg tttgtccgaa gccgaaaaag tcgagtacgc caccaaaatg gcgcacgacg
     2761 cgcaaattta cgacgaaccc gaattcgatc cgcaagagct gcaatcggag tattaccgcg
     2821 gtctttatgc gttcgacaac cgtaaaaaca atgtcgattc ctacaaagcg ctgcattctt
     2881 ggattccctt tatcgcttcg ttcggcgcga tgatgatttc cgctatgctg attttcaagg
     2941 gcttgaaaaa cctgcatttg gggatgagca acgtcaacag cttcctgacc atctttatga
     3001 taggcgcggc ggtgtggatg gggacgtttg tttttgccaa aagcctcaag cgtaaagact
     3061 tgggcaaatc gacctttcag atgttttcat ggatgcaggt ctttaccgcc tgcggcttcg
     3121 cattcagcca cggggcgaac gatatcgcca acgccatcgg tccgtttgcc gcgattatgg
     3181 atgttttgcg taccaacagc gttgccgcgc aaaatgtcgt ccccccgatt gcgatgctga
     3241 ctttcggtat cgcgctgatt gtcggtttgt ggtttgtcgg taaagaggtg attaaaaccg
     3301 tcggtacgag tttggcggaa atgcaccccg cctcgggttt tactgccgaa ctgtccgccg
     3361 catctgtcgt gatgggcgcg tcgctgatgg ggctgcccgt gtccagtacg catatcttgg
     3421 tcggcgcggt actcggtatc ggtctggtca accgtaatgc caattggaaa ctgatgaagc
     3481 ccatcggttt ggcgtgggtc attaccctgc ctgccgccgc cgtattgtcg gttgtctgct
     3541 acttggtttt acaggcagta ttctgattgt aaaatactga tgccgtctga acccgtgttc
     3601 agacggcatt ttgttgatgg aatgtgcggg cttgtgcctt atgcgcaatc tgttctgtcg
     3661 ggatatgccg tttggtatag
//
LOCUS       DAXHMX010000169         3615 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHMX010000169 DAXHMX010000000
VERSION     DAXHMX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.169"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            133..491
                     /locus_tag="ACFM4S_001930"
                     /pseudo
     CDS             133..491
                     /locus_tag="ACFM4S_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            522..3374
                     /gene="gcvP"
                     /locus_tag="ACFM4S_001931"
     CDS             522..3374
                     /gene="gcvP"
                     /locus_tag="ACFM4S_001931"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6051552.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVTLRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAQAEEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTVGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHVVTDMHNASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVERGEWPKEDNPLVNAPHTASDVTGEWTHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
BASE COUNT          850 a         1040 c          937 g          788 t
ORIGIN      
        1 tgaaccgact ggaacaccaa actgcttcgg gacgcatatg ccgtctgaag tgcaaaacct
       61 acgccataca gccgcatgaa gttgcagcgg tatggcgttt tttgaaaaag acggcctgcc
      121 ggttcagacg gcatgaccga ccgtccgagc ctgctgtgga taaagcccgg acaggctgaa
      181 atcatggaat attgcgaacc tgaagaagca tccgacccgt acgcaacata caggcgtgcc
      241 aacctgatgg cgaggctgcc gctgtttgtc gtgattttgg ttctgctcaa tattgttttc
      301 cgcttccggc gcatccctta gcttggctgg tgcctgcagg tttcatggtt ttggtcggcg
      361 gctttccctt atcgctgccg cttgtggcgc tgcttgtcct gacctgctgc attctggcgc
      421 attgtccgcc attatcccgt cttttgtgct acccttgccc gaatcatccg atgtctaaaa
      481 attctgcctg atggcagccc tacaaacccg aaggagtaga aatgaaactg tccgaactgt
      541 tcaaccccga cgaatttgcc gcgcggcatt tgagtttcgg cgacgaagcg gcgttgcttg
      601 ccgctgtcgg cgagaaaagt atggacgatt ttgtcggcaa caccgtgccg caaagcatcc
      661 gtatgccgtc cgaactcgat ttgcccgatg ccctgaccga agcggacgcg ttggcaaaat
      721 tgaaaggcat tgcgtcgaaa aacgtgatca acaagtccta tatcggttta ggctattacc
      781 cgacccgcgt gccgaatgtg actttgcgca acgtattgga aaatccgggc tggtataccg
      841 cctacacgcc gtatcaggcg gaaatcgcgc agggtcgttt ggaagcattg ttgaacttcc
      901 aacaagtgtg catcgatttg accggttttc ccgtggcggg cgcgtctttg ctggacgagg
      961 caactgccgc ggctgaagcg atggcgatgg cgcaccgagt gggcaaagtg aaatccgaac
     1021 gtttctttgt ggatgcgcgc gtgtatccgc aaactttgga cgtgatgaaa acccgcgcca
     1081 aatatttcgg ctttgaattg gtggtcggtg attttgccca agcggaggaa ggcgaatact
     1141 tcggtgcgct gttccaatac gtcggcaaag acggcgatgt gcaagacttg caggacgtta
     1201 tcggccgtct gaaagccaaa ggcacaatcg ttgccgttgc agccgacatt atgagcttgg
     1261 ttttgctgaa gtctccggct gaactaggtg cggatattgc tttgggcaac acgcaacgct
     1321 tcggcgtgcc tatgggcttt ggtggtccgc acgccgctta tttcgcgttt aaagacgagt
     1381 tcaaacgttc cgccccgggc cgcatcatcg gcgtatccaa agacgcatca ggcaaacctg
     1441 ccttgcgcat ggcgttgtcc acccgcgagc aacacatccg ccgcgaaaaa gcgacatcca
     1501 atatttgtac cgcgcaggca ttgctggcga acttggcggg tatgtacgcc gtttaccacg
     1561 gccctgaagg tgtgaaacgc atcgccaacc gcattcacgc gctggcttct gcctttgccg
     1621 atgcgctggt ttcagacggc ctgaatgtgg ttcataaggt cttcttcgat accgttaccg
     1681 tcgatttcgg caacaaagag aaagcagacc aagtgtttgc cgctgctttg gaatcgggct
     1741 ataacctgcg ccgcgttaac gatactcaag ttgcggctgc attccacgaa acatcggcat
     1801 gcgaagattt ggttgatttg taccgcgcgt ttaccggcaa ggatgcgttc gcatttgccg
     1861 atgatgtcaa aggccgtctg aacgccgaat tgctgcgtca agatgacatt ctgcaacatc
     1921 ctgtgttcaa ccgttaccac accgaacacg aaatgttgcg ctatctgaaa aaactcgaag
     1981 accgcgattt ggcgatgaac cgcagcatga tttcgctcgg tagctgcacc atgaaactca
     2041 acgcgactgc ggaaatgctg ccgattactt gggcggagtt ctccgacatc cacccttacg
     2101 ctcccgaagc gcaaaccgtc ggctaccgcg aattgcttgc cgatatggaa aacagcctga
     2161 aagccatcac cggctttgac gcaatttcct tccagcccaa ctccggcgcg cagggcgaat
     2221 acagcggtat gcttgccatc cgccgctatc aggaagcgca aggcgaagcg cagcgcaaca
     2281 tctgcctgat tcccaaatcc gcccacggca ccaaccccgc caccgccgcc atgctcggtt
     2341 tgaaagtcgt cgtcgtcgat accgacgaac acggcaacgt caacatcgac gatttgaaag
     2401 ccaaagccga gcaacaccgc gacgctttgt ccgccatcat gattacctat ccgtccaccc
     2461 acggcgtgta cgaagaaggc atccgcgaca tctgccgcat catccatgaa aacggcggac
     2521 aggtttatat ggacggcgcc aacctcaacg ctcaaatcgg catcatgcag cctgccgaag
     2581 tcggcgcgga cgtgttgcac atgaacctgc acaaaacctt ctgtatccct cacggcggcg
     2641 gcggcccggg catgggtccg attggcttga aagcccatct ggctccgttc gcaccaggcc
     2701 atgtggtaac cgatatgcac aacgccagtg ccgatcaaac tgccgtggct gccgcagctt
     2761 tcggttctgc gtccatcctg ccgattactt ggatgtacct gaccatgatg ggcaaacaag
     2821 gcatggagca ggcaacacgc tgggcattgc tcaacgccaa ctacgttgcc aaacgcttga
     2881 gcgaagacta tccgattctg tatacaggca aaaacggccg cgtcgcgcac gaatgtatcg
     2941 tcgacttgcg tccgctcaaa gccgaaagcg gcattaccga aaccgacatc gccaaacgcc
     3001 tgatggacta cggcttccac gccccgaccg tctccttccc cgttgccggc acgctgatga
     3061 tcgagccgac cgaaagcgag agcaaagccg aactcgaccg cttcatcgcc gccctgaaac
     3121 aaatcaaaca ggaagtgttg aaagtcgagc gcggcgaatg gccgaaagag gacaacccgt
     3181 tggtgaacgc tccgcatacc gcatccgacg taactggcga atggacgcat ccgtattccc
     3241 gcgaagaagc cgtcttccca ttgccgttcg tccgcgaaca caaattctgg ccgagcgtca
     3301 accgtgtgga cgacgtgtac ggcgaccgca atctcgtgtg cagctgccca ccgatggaaa
     3361 attatgaaga ctgaccgttg aaaccccgaa gacaaatgcc gtctgaaaca ctttcagacg
     3421 gcattttcgt cgacggcaga ctggttgcac caatacacgt atctcgacta taactttaaa
     3481 acaaatgagt taaaccagta tccatacatc agctttttta tcatcctact ttttattcat
     3541 ccgatcgtgc aaacagattt caaagatgaa aagcctattc acaccctttg atgtcatttc
     3601 cacacggaca aacaa
//
LOCUS       DAXHMX010000170         3564 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHMX010000170 DAXHMX010000000
VERSION     DAXHMX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.170"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..215)
                     /locus_tag="ACFM4S_001932"
     CDS             complement(<1..215)
                     /locus_tag="ACFM4S_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6051553.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRISLSAANIEAENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKT"
     gene            complement(462..845)
                     /locus_tag="ACFM4S_001933"
     CDS             complement(462..845)
                     /locus_tag="ACFM4S_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051554.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(850..1687)
                     /locus_tag="ACFM4S_001934"
                     /pseudo
     CDS             complement(850..1687)
                     /locus_tag="ACFM4S_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase domain-containing protein"
     gene            complement(1986..2381)
                     /locus_tag="ACFM4S_001935"
     CDS             complement(1986..2381)
                     /locus_tag="ACFM4S_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051555.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(2393..3106)
                     /locus_tag="ACFM4S_001936"
                     /pseudo
     CDS             complement(2393..3106)
                     /locus_tag="ACFM4S_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(3202..3474)
                     /locus_tag="ACFM4S_001937"
     CDS             complement(3202..3474)
                     /locus_tag="ACFM4S_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051556.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
BASE COUNT         1023 a          727 c          608 g         1206 t
ORIGIN      
        1 gttttgtagc tgcggcgttc aaaggtatag agttcgctct gtctcgcata gtaatattgg
       61 tcaccgtaag attgaatttt gattttgccg ttttcagctt cgatgtttgc cgcactcaga
      121 ctgatgcggc tgttctcatt ggcatacggt gcgctaatgc tcacaccggt tttacccgaa
      181 agttctgcag caagcgtagg gggtgtcttc agcacgcacg cgtctgggca gtatataggg
      241 aatctgaata tttacttgca taacaaatgc cgtctgaaaa attgtgagct tttcagacgg
      301 cattgagccg taaatcatgg aacgcgtgcg tgctggagca cacaccttac gcatggattt
      361 taggtttcat gcaggctaca gcttgctgct gcggtataaa agtattttta gtgatttact
      421 gtttttggta tttttgcccg acggggttaa aaatacagtt gctactcttg ctgcatgata
      481 ttggcaagat ttaatttaaa tgtttgttga atcttgttta catccttagc aaaaatatga
      541 tagtcactcg aagaatagat agattttatt ctatccaaat tagtttgttg ataacatttt
      601 ttcatttccg cgatgaaaaa attatctttt cctctttcat tgagacagtt attttctaac
      661 agatagattg cccatgctgt aaaaataaat gtactatctt ccggatattc agataaataa
      721 atttcgaatt caattataga atattcagct gcttcaatta atgttatatc taaatccctg
      781 acaaattttt ccgttatatt tttaaataat ttcttgaatt ctatataatt ttttattttt
      841 tccatgattc tattttcctg taaaaatatt taattgaata tttggatagc gatttctaaa
      901 ctctaaaata acattgctgc aagattgaca ggcctttaat tctgtaaata gatcaattct
      961 acctgataca ttacgattat ttccgagttg ctgtgctata gtttcaagta atttatattc
     1021 tccatctata tttcttaatg ttcctctagg aggagaggca atattatgat atttatcaac
     1081 agatataggt ttaaaaattt ttgtttcagg taacgaaata aatccatgtt cccctttttg
     1141 gaaagaacta aatgcttcca tcctttgcgg taatccttta acatcaattt ttgctatcgc
     1201 aagattccct atttgtcgat actcttccgg cagtttcatg cggactctct ggctttcagc
     1261 agttaaaagc aggagcactg tcattgctca gataacgggt aacaggtgta ggcttggccg
     1321 atattgcagc ctgtgcagat acagatcctg caaccatcaa tgtgccatta acaacctgaa
     1381 ctgtaccatg cagatattct ccattagaga ttgctttcgc tccctgtcca atgttatatc
     1441 cccctgctgc cacagtagct gctttgacac tgaagccaag cggtccgttt aataagtaaa
     1501 gtgcaggttg tgcggcaaca gctgatttgt aatcaaaaga atcataacga ctcaatgttt
     1561 gataggcttt cgataaagtg ttattaatat ctttggaagg aatagtgaga ttacccattg
     1621 actggggcgt tttcagtatc gaagctgccc aagctgtgtt acctccagag gcttggttaa
     1681 taaaatatgt tacctatgta tattgtttct tcaaaaatca accgtattgt ccttgggtga
     1741 ttttcgtacc agcacaatca gtctgcccta gttgacagat cgcgccacac tagccagata
     1801 cagcgtatca ttggtaaaac tgataatgcg gcaatataaa gaaaaacttc aatagtatgt
     1861 taacgaaatt tgacgcataa aaatacgcta aaaaattttc gcttgactaa ttaaaaatgg
     1921 gattgagcat aaaattttaa taatttatat tattgaaaat atctcagatt ttcagtcagt
     1981 ttattttaca agccgaaccg taggctattt tccatcttta accatgaata gacctcaact
     2041 tcatcataat ttgtgcctgc aatagcaact tccagaaagt caataattct acctgcttta
     2101 ttcaaagact ctgtagtata tagcaggaaa gtcgctgcat ctgacttatc cggtgcagtg
     2161 atgtctcctt caaaaaaaat agggatattt tcgtatttat ttagattagt tctttctaaa
     2221 tttacctgaa aatcaatgta gcacccacat cctgtattat ctctagatac tattattgaa
     2281 ttttctattt gctctattaa atattccttc catatcatat tgttttcaat aattctacat
     2341 aagaaagcct tctcatattc agataaacaa aactggttca tatattctac ccttacattt
     2401 tcaaaatagt tgcaatacga gatactccat tgttgacata tacgcgaaca attaccgtta
     2461 taccattgac cgttacagta actggataat ttcctgtttt tgtcagtttt aaagcatcca
     2521 ctgccttttg cgtcgctaca acagcatttt ccatggagcc tgatttttga atcaatgtat
     2581 taccgatgct tccttgatgc cccttaaaga tgtgatgaac tttatttagc caatctacac
     2641 cgtatctttc agccaatagt cgagaattac tttcacctat tttttccatc gaagctaaaa
     2701 tcctttgagc ataggcagca tcagctgttg aaaaatttgc ttcgcccaac aaaagccgta
     2761 attcctgaat atctcctcgt aaaattgcat ttcttactcc cgcttctaat gtggtttgtg
     2821 gctgaaagag ggaattattc tctaccgcca cagtagccgc attcgccgct gtattcacat
     2881 ccccgcctac aacggcagac gcagtgccgg caaccagttt tgaataggca gtaatcttcg
     2941 ctttagcttt ttcgatttcg gtcgcactca tacgactgaa atcagtattc ttaaccaaag
     3001 cctcaccaac aatctcaccg actgcagcgc ctatcgcacc atcctgacac ttacccttat
     3061 tcgccgccgc tgccgcacag cccgctatgg catgggcaat cttgtgggtg gtatatcgct
     3121 gccagccctc tctgttgata atcttgcgta tgattacgcc gtttgatggc taatcgtacc
     3181 tacgcaggct taaaatttcc atcaatccat atcatttgag ttttcatctt cataatatgg
     3241 atgatcaaac aaatctcctt cccaaataca ttcactcgtt ataccttggg attccatgaa
     3301 aggaacataa gaataaaaat ctttttcata ttctccataa aatatattca tacctttgag
     3361 ccatactgca tcgcattcat cacacagaat aatttcatca tgaagatatt taggtttcga
     3421 atggtagaga tagccttgtt gacaagcagg acacattttc attctcttat tcatgattac
     3481 tccatataat cgatttattt ttgtgggtaa gcagtagtga tttgattgct gcctttagtc
     3541 tgtacaacaa tacgtatata gcaa
//
LOCUS       DAXHMX010000171         3536 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHMX010000171 DAXHMX010000000
VERSION     DAXHMX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.171"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..3025)
                     /gene="pilC"
                     /locus_tag="ACFM4S_001938"
     CDS             complement(<1..3025)
                     /gene="pilC"
                     /locus_tag="ACFM4S_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6051557.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGGAQAQTSKYAIIMNE
                     GNQLEVKRNGQYSTIKDRDREREYTHHNHRQGGGSVSFNNSDELVSRQSGTAVFGTAT
                     YLPPYGKVSGFDADGLNKRGNAAGWIRTTRIALAGYSYEGVVCRSGTGCPKLVYKTRF
                     SFDNPDLVKNAGRLDRHTDPSRENSPIYKLKDYPWLGVSFNLGAEGTTKDGKTINKLV
                     SSFDEKNSSNNNLVYTTEGRDISLGDWQREKTAMAYYLNAKLHLLDKKGIKDITNKTV
                     QLGVLRPSIDVRLQRNTGLAGLLNFWASWDIKDNGQIPVKLGLPEVKAGRCINKANPN
                     PKAQALSPALTAPALWFGAGQDGKAEMYSASVSTYPDSSSSRIFLQNLKRKNDPNKPG
                     RYSLADLSASDIQSKEPSFTSRQTIIRLDGGVHEIKLDRSNEATGLNGNDGKNDTFGI
                     VSEGSFMPDTSEWKKVLLPWTVRASNDDGQFNTFNKEENNGKPKYSQKYRSRDSSKHE
                     RNLGDIVNSPIVAVGGYLATSANDGMVHIFKKGNGVDERNYSLKLSYIPGTMPRQYFD
                     NDTSALKDSTLAKELRAFAEKGYVGDRYGVDGGFVLRQVERNGKDHVFMFGAMGFGGR
                     GAYALDLTKADGNDPTKASLFDVKDNGNNGNNRVELGYTVGTPQIGKTHNGKYAAFLA
                     SGYATKEINSTENQTALYVYDLESNNGTPIATINVPDGKGGLSSPTLVDKDLDGTVDI
                     AYAGDRGGSMYRFDLSGNNPTSWSARAIFSGNKPITSAPAISQLKDKRVVIFGTGSDL
                     SEDDVLSTSEQYIYGIFDDDMVANNVNVKLSGLGGGLLEQHLTQEDKTLFLTDYKRSD
                     GSGDKGWVVKLEAGQRVTVKPTVVLRTAFVTIHKYTGNDKCGAETAILGINTADGGKL
                     TKKSARPIVPDANKDVAQYSGHKKGTNGKSIPIGCMEKNGGTVCPNGYVYDKPVNVRY
                     LDEKKTDGFSTTADGDAGGSGIDPAGKRAGKNNRCF"
BASE COUNT          708 a          952 c          918 g          958 t
ORIGIN      
        1 agaagcagcg gttgtttttg ccggcacgct tgccggcggg gtctataccg ctgccgcccg
       61 catcaccgtc tgccgttgtt gaaaatccgt ctgttttctt ttcatccaga taacgcacat
      121 taaccggttt gtcgtaaaca tatccgttcg ggcagacggt tcccccgttt ttttccatac
      181 aacctatagg gatggatttg ccgttggtgc ctttcttatg accggaatat tgcgcgacat
      241 ccttattggc gtccggcaca atcgggcgcg cgcttttctt cgtcagcttg ccgccgtcgg
      301 cggtattgat gcccaaaatg gcggtttccg cgccgcattt gtcattaccc gtatatttat
      361 ggatggttac aaaggcggta cgcaatacca cggtcggttt gacggtaacg cgctgtccgg
      421 cttccaattt cactacccag cccttatcgc ccgagccgtc ggatcgcttg taatcggtca
      481 ggaataaggt tttatcttcc tgagtaaggt gttgctcgag cagcccgcct cccaaaccgc
      541 tgagttttac atttacgtta ttcgccacca tatcgtcgtc gaagataccg taaatatatt
      601 gttcgctcgt actgagtaca tcatcctcac tcaaatcact gcccgtgccg aagataacca
      661 cgcgtttgtc tttcagttgg gaaatggcgg gcgcggaagt aatcggtttg ttgccgctga
      721 aaatggcgcg tgcagaccaa ctggtcgggt tgttgccgct taaatcaaag cggtacatac
      781 tgccgccgcg atcgccggca taggcgatat caaccgtgcc gtccaaatct ttatccacca
      841 gcgtggggga cgaaagcccg cccttgccgt cgggtacgtt gattgttgca atcggcgtac
      901 cgttgttgct ttccaaatca tacacataca gcgcggtttg attttctgtg ctgttaattt
      961 ctttagtcgc ataaccggag gcgaggaagg cggcgtattt gccgttgtgg gttttgccga
     1021 tttgcggcgt gccgacggtg tagcctaatt ccacgcgatt attgccatta ttgccgttat
     1081 cttttacatc aaacaaagag gcttttgtcg ggtcattgcc gtcggctttg gtcaaatcca
     1141 aggcgtatgc gcctctgccg ccaaagccca tcgcgccgaa cataaacaca tggtctttcc
     1201 cattccgttc gacttggcgc aagacaaagc cgccgtccac gccgtagcgg tcgcccacat
     1261 agcctttttc ggcaaaggcg cgcagctctt tggcaagggt ggagtctttg agagcggaag
     1321 tgtcgttatc aaaatattgg cgcggcatcg tgccggggat ataactgagc ttcagactat
     1381 agttgcgctc gtccacgccg ttgccttttt tgaagatatg caccatcccg tcgttggcgg
     1441 aagtagccaa atacccgccg accgccacga tggggctgtt gacgatgtcg cccaaattgc
     1501 gctcgtgctt gctgctgtcg cggctgcggt atttttgact gtattttggc ttgccgttgt
     1561 tttcttcttt gttgaatgtg ttaaattgac cgtcatcatt ggaagcacga accgtccaag
     1621 gcagcaatac ttttttccac tcgctggtat caggcatgaa gctcccttca ctaacaatgc
     1681 cgaaagtgtc gtttttgccg tcatttccat ttaaaccggt ggcctcattg cttctatcca
     1741 gtttgatctc atgtacaccg ccatccaatc ggatgattgt ttgccgactt gtgaaactcg
     1801 gctctttact ttgaatatcc gacgcactca agtctgcgag ggaatagcgg ccgggtttgt
     1861 tggggtcgtt ttttcttttc agattttgaa ggaagatgcg gctgctcgaa ctgtcggggt
     1921 aggtggaaac cgaagcggaa tacatctccg ccttaccatc ttgcccagct ccgaaccaca
     1981 gcgcgggggc ggtcaaggcg ggggaaaggg cttgggcttt gggattgggg ttcgctttgt
     2041 tgatgcagcg gcctgctttg acttccggca ggccgagctt gaccggaatc tgcccgttat
     2101 ctttaatgtc ccaactagcc caaaaattta gcaagccggc aagccccgta tttctttgca
     2161 gccgcacatc gatgctcggc ctcaagacac ccaactgcac tgttttgttg gtgatatctt
     2221 taatcccttt tttgtccagc aggtgcagtt tggcgttcag ataataggcc atggcggtct
     2281 tttcgcgctg ccagtcgccc aaggaaatat cgcggccttc cgtggtatag acgaggttgt
     2341 tattactact attcttttca tcaaaagaag ataccaattt gttgattgtc ttaccatctt
     2401 tggtggtacc ctcggcaccc aaattgaaag atacgcccaa ccatggataa tccttcaatt
     2461 tgtaaatggg cgaattttcg cggcttgggt ctgtgtgcct atccagcctg cccgcatttt
     2521 ttaccaagtc gggattatcg aaggaaaatc gggttttata gacaagtttg ggacagcctg
     2581 tgccgcttct gcatacgaca ccttcgtagc tgtagcctgc cagcgcgatg cgggtggtac
     2641 gaatccaacc ggcggcattg ccgcgcttgt tcagcccgtc ggcatcaaaa ccggaaacct
     2701 tgccgtaggg cggcaggtag gtggctgtgc caaaaacggc agtaccgctt tgtcgagaaa
     2761 caagctcatc gctattgttg aatgagacag agcctcctcc ttgtcggtga ttatgatgag
     2821 tatattcgcg ctccctgtct ctgtccttta ttgttgaata ttgcccattc cgctttacct
     2881 cgagctggtt tccctcgttc atgataatag cgtatttact ggtttgcgcc tgcgcgcccc
     2941 cccccccgcc ggtatgggaa aacatcaaga tggcggcata aagcgcggta tggcggaaaa
     3001 cctgcctttt taaagtttta ttcatcgtat ttccttttcg gtcaaacccc ccgcccttcg
     3061 ggcatccgtc cttcggggcg gcaggatcag gctttgtccg ggaggggtgc aagcccttcc
     3121 gaatcagggc ggcgccttat gtgccgccct gcgtgttgga acatatttta tcccttaaat
     3181 cttgcttcgc gggataccgt cggcggcagg cgcgccgcaa acggcgggat tgggcaaacc
     3241 gcccgattcc gcgcccgccg aattgctggt tggaaaaccc ctttggcccg gctgccaaag
     3301 ggggatgttc ggaaaagtaa cacgcccctt tggccggatg caatgtatag ggttaccgca
     3361 ttattctacc cttgcgcgct gatcatggta aagcggtttt cggcaatccg caaccgcctc
     3421 cttggcccgc cattgccgcg aaagcgggaa tccggaacgt aaaatctcaa aaaaaccgtt
     3481 ttatccggca agtttgcccg ccggcaggcc cgccattgcc gcctgcgcgg caatgg
//
LOCUS       DAXHMX010000172         3523 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHMX010000172 DAXHMX010000000
VERSION     DAXHMX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.172"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            186..1061
                     /gene="dapA"
                     /locus_tag="ACFM4S_001939"
     CDS             186..1061
                     /gene="dapA"
                     /locus_tag="ACFM4S_001939"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6051558.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADFTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARKLNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1070..2266
                     /gene="bamC"
                     /locus_tag="ACFM4S_001940"
     CDS             1070..2266
                     /gene="bamC"
                     /locus_tag="ACFM4S_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6051559.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTLIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2323..3255)
                     /gene="pip"
                     /locus_tag="ACFM4S_001941"
     CDS             complement(2323..3255)
                     /gene="pip"
                     /locus_tag="ACFM4S_001941"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6051560.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRNIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDILPRLL"
BASE COUNT          930 a         1158 c          788 g          647 t
ORIGIN      
        1 cgacggcacc ggcataaacc ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat
       61 ttccaccata cctttcggcg caaacgcccc acatctgcaa cccgccggat ttcacacctg
      121 cggtaaagta ggctacaatc agaagcatta ccgcccccta caccacaact gataaaaggt
      181 tctgtatgtt acaaggtagc ctggttgccc tgattacccc gatgaatcaa gacggcagca
      241 tccattacga acaactccgc gatttgatcg actggcacat tgaaaacggc acggacggca
      301 tcgttgccgt cggcacgaca ggcgaatccg ccaccctctc cgtcgaagaa cacaccgccg
      361 tcatcgaagc cgtcgtcaaa cacgttgcca aacgcgtccc cgtcatcgcc ggcacagggg
      421 caaacaacac cgtcgaagcc atcgcccttt cccaagccgc cgaaaaagcc ggtgcagact
      481 tcactctctc cgtcgtcccc tattacaaca agccctccca agaaggcatt taccaacatt
      541 tcaaaaccat cgccgaagcc acttcgattc cgatgattat ctacaacgtg cccggccgta
      601 ccgtcgtcag catgactaac gacactatcc tgcgccttgc cgaaatcccc aacatcgtcg
      661 gcgtgaaaga agccagcggc aacatcggca gcaacatcga actcatcaac cgcgcgcccg
      721 aaggcttcgt cgtcctttcc ggcgacgacc ataccgccct gccgttcatg ctgtgcggcg
      781 gacacggcgt gattaccgta gccgccaacg ccgcacccaa gctctttgcc gatatgtgcc
      841 gcgccgcgct gcaaggcgac attgccctcg cacgcaagct caatgaccgg ctgattccga
      901 tttacgacac catgttctgc gaacccagcc ccgccgcacc gaaatgggcg gtatccgccc
      961 tgggcagatg cgaaccgcac gtccgcctgc cgctcgttcc gctgacggaa ggcggacagg
     1021 caaaagtccg cgccgccctg aaagcctcag gacaactcta aatataacta tgccgtctga
     1081 accgttcgga cggcataacg caacaaacac tttaatatcc atcacacagg atgacacgat
     1141 gacccttatc aaacccgtca ttgccgcgct cgcactcatc gggcttgccg cctgctccgg
     1201 cagcaaaacc gaacagccca agctcgacta ccaaagccgg tcgcaccgcc tgatcaaact
     1261 tgaagtccca cccgatttga acaaccccga ccaaggcagc ctctaccgcc tgcctgccgg
     1321 ttcgggagcc gtccgcgcca gcgatttgga aaaacgccgc acacccgccg tccaacagcc
     1381 tgccgatgcc gaagtattga aaagcgtcaa aggtgtccgc ctcgagcgcg acggcagcca
     1441 acgttggctt gtcgttgacg gcaaatcccc tgccgaaatc tggccgcttc tgaaagcctt
     1501 ttggcaggaa aacggcttcg acatcaaatc cgaagaaccc gccatcggac aaatggaaac
     1561 cgagtgggcg gaaaaccgtg ccaaaatccc ccaagacagc ttgcgccgcc tattcgacac
     1621 agtcggtttg ggcggcatct actccaccgg cgagcgcgac aaattcatcg tccgtatcga
     1681 acagggcaaa aacggcgttt ccgacatctt cttcgcccac aaagccatga aagaagtgta
     1741 cggcggcaaa gacaaagaca cgaccgtatg gcagccctcc ccgtccgatc ccaacctcga
     1801 agccgctttc ctgacgcgct ttatgcaata tttgggcgtt gacggacagc aggcggaaaa
     1861 cgcatcggca aaaaacccga ccctgcccgc cgccaacgaa atggcgcgta tcgaaggcaa
     1921 aagcctgatt gtctttggcg actacggcag aaactggcgg cgcaccgcgc tcgccctcga
     1981 ccgcatcggg ctgaccgtcg tcggtcaaaa caccgaacgc cacgctttcc tggttcaaaa
     2041 agccccgaac gaaagcaatg cagttaccga acaaaaaccc ggcctgttca aacgcctgct
     2101 gggcaaaggc aaagcggaga aacctgccga acagccggaa ctgattgtct atgccgagcc
     2161 tgtcgccaac ggctcgcgca tcgtcctgct caacaaagac ggcagcgcat atgccggcaa
     2221 agacgcatcc gcattattgg gcaaactcca ttccgaactg cgttaaacca ttcaaaacaa
     2281 aagcagcttc caaacagaag ctgctttttt atgcggaact ttttacaaca aacggggcaa
     2341 aatatcctca accgcctgaa ccagcgcatc cgccaaaggc ggatcgaacg cacgatgccc
     2401 tgcctgaacc accctcaatt ccgcttccgg aaaggctttc gacagcgccc acgcactctg
     2461 catcggcgta cacaaatcat accgcccctg cacgataatg gtcgggatat tccgtatttt
     2521 gccgatattg ttcaaaatcg ccctatcgcc ctgcaaccag ccgccgttga caaaataatg
     2581 gttttccaaa cgcgcgattg ccagcgaggc ataagcatct tcatccactt cctccggctc
     2641 gaaacggatc agatagcttt cccaatccgc ccaagccttt gcggcagaca ggcacacttc
     2701 ttcatcttga tgaaacagca acccgtgata cgcctcaatc agccggttcc gtcgattttc
     2761 agcaatcggc gcgacaaatt tttgccattg ttccggataa atccggctca caccgcccgc
     2821 ctcgttcagc cacgccgttt cagacggcct gcacaaaaat atcccgcgca ataccaatcc
     2881 ctttacccgt tcaggatggg tttgggcata agccagcgac aaagtgctgc cccacgaacc
     2941 gccgaacacc agccatttcc tgatacccag catttcacgg actttttcaa tatccgccac
     3001 caaatcccaa gtcgtattgt cttccgcaca agcatacggg cgcgaacgtc cgcaaccgcg
     3061 ctggtcgatg atgacgatgc ggaacacatc gggattgaaa aaaccccgac attcaggcga
     3121 agcccccgcg cccggcccgc catgtaaaaa aataaccggc acaccgtcgg gattgcccga
     3181 ttcctcccaa taaatttgat gaatttcaga cacctgcaag tatccgctat gaaaaggctg
     3241 ttttatctca tacatcatct tctcccatat tgcttctgac aatccgaaca ttcccacacg
     3301 caacatcacg cacgagacac aagccgcccc gaaaggcaaa tgccgaaaaa tcgcgatata
     3361 gtgaattaac aaaaatcagg acaaggcggc gagccgcaga cagtacggat agtacggaac
     3421 cgattcactc gtgcttaagc accttagaga atcattctct ttgagctaag gcgaggcaac
     3481 gccgtaccgg tttttgttaa tccactataa cagcaaccct gtc
//
LOCUS       DAXHMX010000173         3478 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHMX010000173 DAXHMX010000000
VERSION     DAXHMX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.173"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            171..680
                     /locus_tag="ACFM4S_001942"
     CDS             171..680
                     /locus_tag="ACFM4S_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051561.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRSRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(733..1080)
                     /locus_tag="ACFM4S_001943"
     CDS             complement(733..1080)
                     /locus_tag="ACFM4S_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6051562.1"
                     /translation="MYIPDKGDIFHLNFDPSGGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSGGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1082..1318)
                     /locus_tag="ACFM4S_001944"
     CDS             complement(1082..1318)
                     /locus_tag="ACFM4S_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC6051563.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            complement(1677..2168)
                     /locus_tag="ACFM4S_001945"
     CDS             complement(1677..2168)
                     /locus_tag="ACFM4S_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC6051564.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACEIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2420..2590)
                     /locus_tag="ACFM4S_001946"
     CDS             complement(2420..2590)
                     /locus_tag="ACFM4S_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051565.1"
                     /translation="MPSEIQTAFLLKGFSSTALHKGGFFMPSESPSDGIGLHGRSSPA
                     FKQERMSRMPDF"
     gene            complement(2606..2974)
                     /locus_tag="ACFM4S_001947"
     CDS             complement(2606..2974)
                     /locus_tag="ACFM4S_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFC6051566.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
     gene            complement(2971..3102)
                     /locus_tag="ACFM4S_001948"
     CDS             complement(2971..3102)
                     /locus_tag="ACFM4S_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051567.1"
                     /translation="MQNQNTRPVKIELKGEAGKRVLLAAARRIAKTHQKAVKALADK"
BASE COUNT          889 a          843 c          799 g          947 t
ORIGIN      
        1 gcgctgaaat tcgaccagtt gatactcgaa tttcccgagc gcggggacgg cgcatgggta
       61 cacatcggtt tccgacgcaa cagcccgcaa cgcaaccaga tactgaccgc aaccaagaaa
      121 aacggcaaaa ccgtgtatct gcccgggctg catccttgag gtcatctgaa atggatattt
      181 tattgaaata ctggaagccg gtaggcgtat tgctgctgat agtcctgatt tttaccgcat
      241 ggcatttcga ccgtgccgaa aaataccgca tgggacggga ggctgctgct gccgaaattt
      301 cgaatcgtct gaaagacggc tatatcgagc aggcaaagca ggcgcgttct gccgagcaga
      361 aggccgctgc cgcgtttgcc gaacgacaaa ccaaattaga agaggaaaaa caaaatgctg
      421 aaaaaactgt tgccgctatg cgtcttgagc ttaaccgcct gcgccactac gccgccgccc
      481 aaaatcgcag cagaaacctg cccgcaacca ctaccgccgc caccgcatct gatggcgcgt
      541 cagattccca aggctggcta ttattcggac agtgcgctga aaaatatgca ggactggcag
      601 aaacagccga cggacatgca gccgatttaa gggaatggca ggcatatggt catgcagtaa
      661 gcagtcagcg tgccgaataa gcaaccgccc gaacctgtaa gaaaagatta caggttcggg
      721 cggtttcagc atttaatcga ataagacggc gccgatgcgc gccagcacat cgtccaatac
      781 ataatcgggt acagtttctt taaaagaagc cttgcggatt tgccagtcca gagatttgag
      841 ctggtggcag tggacattgc cctgcgtttc cgttcctgca ccgagtaagg ttgaaatcat
      901 gccgccgctt cgtgcagccg ctgcattccc ctgtgaaatg gggcaggcaa aaaccaatcc
      961 cgttgcgcgg ttgaatgctt ttggagacag agccagcgca aaccgcccgc ccttgatttc
     1021 cttgccgccg gaagggtcga aattcaaatg gaaaatatcg cctttgtcgg gaatatacat
     1081 ttcagacgac ctcgttgccg acatcatcca agatttccca gccttctacg cgcggcgggg
     1141 tttcttccat ttcggcaagc aagtctgcca agcggaaacg tcgggcagca cgcacacgga
     1201 gttcgccgtt atgtacttcc gctaccaaag cgtcgccgat tttaaaatcc aattgtttca
     1261 gcatgtcggc aggcagtcgg acggccgccg agttccccca tttttggaca cgcaacataa
     1321 tcttcacctt tattgtatct acaaagtaga tacatattac cataaaattt cagttgttca
     1381 aatacttgtg cagaatacgc caaaagccgt ccgaactgtt tcggacggct tttgtactgt
     1441 atttgcgcct tcaggcaata ttttgttatc cattttcaag atgcaaaagc tttctaattg
     1501 cttgatgtcg gatttcggtt gtttagggat acaaaaccaa gtaaactaaa actgtttgat
     1561 tggaaaatgc tccgcaagga gaaaattatg ttcaaaaaat cactttataa ggctgctttg
     1621 gcgtatttcg gcgattgcgt ggctgcccat atttcagaac agttttgact gtttattcac
     1681 aaatcagatg cctttagggg gtttgctttc cataatgcaa ccaaaatttc caactctcta
     1741 aatattgtgt ctttgcgttc ttcttcgcgt attttcataa caagaggcga aactgcattc
     1801 cacaatttta tgaagttggt gcaatgcagc cgtttaaaca aatcctcatc caatactccc
     1861 gagttgattt cgcacgcata aaactcgtgc cgattgatta cggtaagtaa atgccctctc
     1921 tctttttccc attgtccgcc cttattttga taaatttcat acaggtcgat ccgcgcgttg
     1981 ttgtcgtcaa ctgccgtgcc gcgaatataa ggcgaaatat gattgtcggc ttctacaaat
     2041 tgtgcatctt ggtaatcatt caaaataaca tctaatgtcg ccttttgctt tgaagttttc
     2101 ttattgatga atattgtccc cagtgcaatg acggcggtta ctgaaacaac ggtcagttgc
     2161 caaaacatca gccattcggc cgtccctcat aggttaaact gtacgctgtg cctgaatatg
     2221 taaaataaag tgaatacaga tacaaacaag gaggttaggg caattactga caaccaacct
     2281 ccttgtttta tttgtttccg caccttacca gccattaaaa ccctcatttt tgtatttcat
     2341 cgtatttcct taatggttgt aaccttgctt gtcgatatct gtatattaat gccgtgcgcc
     2401 cgttctttca aggggtgatt taaaaatcag gcatccttga catcctctcc tgcttaaagg
     2461 cgggggaact cctgccgtgt aaaccaatgc cgtctgaagg gctttcagac ggcataaaaa
     2521 aaccgccttt gtgtaaagcg gttgaagaaa agcctttcaa taaaaatgcc gtctgaattt
     2581 cagacggcat tgttgtcgga tatgcctatt ccttatccag tcggcgcagg gtttcggcga
     2641 gctgtttgaa gtcggtttcc cccgccgcca aactgacaat caagtcgtca agcccttggt
     2701 cggcggcaat gctgatgccc tgcaaatcca gataggtcaa cattgttaaa agcgcggtgc
     2761 gcttgttgcc gtcgggaaag gcgtgggctt tggctatggc ttgtgcataa agggcggcga
     2821 tttcgtagat gtcctcaagg ttttcatact gccgccagtt ggcaatccgc gacaatgcgc
     2881 cgtcgagccg cgccatatcc gcccgccctt tcaaacccgc ttcatccgcc aatacggttt
     2941 gatggataag cgcgaccagt tcgccgtcta tcatttgtcg gcaagtgcct tgacggcttt
     3001 ttgatgggtt ttggcaatgc ggcgggcggc ggcaagcagt acgcgtttgc ctgcttcgcc
     3061 tttaagctcg attttgacgg ggcgtgtatt ttggttttgc atcgctgctc cttgtctgtt
     3121 tgcgggcatt ttagcttttt tccggcagct tgggaaatgc cgtctgaaaa cacttcagac
     3181 ggcattcttc taatagtgta atgcaatagt tactccaaga tttttgtata tggctgtttt
     3241 tatggtaaat taaattttca caaagaataa atatatttaa atcaataaaa taaaatttag
     3301 tcgaatccca ccgtttccgc cagaaaacaa aaccgccctg attcagggcg gttctttttg
     3361 gcgcgaatct gcacaattac cataaaagaa tcattggatt atgcttattt tccgtaacgc
     3421 cctgcaataa accgcaatcg gcagatgctt tgatcacagt tttatggtaa tttatatg
//
LOCUS       DAXHMX010000174         3475 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHMX010000174 DAXHMX010000000
VERSION     DAXHMX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.174"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1017..1787)
                     /gene="xth"
                     /locus_tag="ACFM4S_001949"
     CDS             complement(1017..1787)
                     /gene="xth"
                     /locus_tag="ACFM4S_001949"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6051568.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1847..2128)
                     /locus_tag="ACFM4S_001950"
                     /pseudo
     CDS             complement(1847..2128)
                     /locus_tag="ACFM4S_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
     gene            complement(2267..2506)
                     /locus_tag="ACFM4S_001951"
     CDS             complement(2267..2506)
                     /locus_tag="ACFM4S_001951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051569.1"
                     /translation="MSDKEPPNRNPVIASRRKCRLKYSSDGIFREGRDALEKTAFQAG
                     ILFARQRGGIGRAGERTGAAVLCCLKARLYACLQK"
     gene            complement(2534..3415)
                     /gene="nadC"
                     /locus_tag="ACFM4S_001952"
     CDS             complement(2534..3415)
                     /gene="nadC"
                     /locus_tag="ACFM4S_001952"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6051570.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPEKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
BASE COUNT          848 a          837 c          870 g          920 t
ORIGIN      
        1 ctgatgcaga tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
       61 caaatagtac ggcaacgctg tactggttta aatttaatcc actatatttt gtaatagctg
      121 ccattatgcc tactaggata tataccttct atataatcct tatttaaata aaaaccctta
      181 taaaaatctg tgttatctgt cgggattgaa aaaaagattt tatctttctc atcaggttta
      241 tatttgctat caatatcaaa aagagaataa ggattacaat gagttcgaca tactaggtaa
      301 aaatttatat cttggatcat gaaatattcg atatttatca ggatcgaaag atgctaaaaa
      361 ttcatcaata tcggtactgg atattttgtc atcactaata aacatcgcta gagatgggcg
      421 actttgattt tccatttgtt gattagtaaa ggatataaat actaacttgc cattgtcaaa
      481 ccacatagat ttatatagtg gattaaattt aaaccagtac gtcgttgcct cgccttgccg
      541 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatag
      601 ttattgcctt tgggagaatc aattctgaca gcacataaca taggttcatc tttacagtcg
      661 ttatattggg tttcatcaaa ctcaactgca tagcacaata aaggcaatag gaaaaaaagg
      721 agattaattt attactatac ataaggcagc ctgactattc attctaaaat aaatccggtt
      781 tttaatattt gattctgctc cggattatat acataataaa ttttactgct ccaaaatctg
      841 atattaaccc tatggatttc ataaattttt cagaaaaata ggctgtgctt gaacaatccg
      901 ccgactccgg ctgtttgtca gggaaaaggc aattattttc ggttagtcag cagcctatcc
      961 tattgaatca cttaaagata tggatgtttg tcaacgccat atcaaaacac ggtcttttac
     1021 caatcgaatt ctgccgtcac cggcgcgtgg tcgctcggac gctccagcgc gcgcgtctcc
     1081 aaatcaacgc ggacatcctt caacgccgcc gccatcgcag gcgacaccaa aatatggtcg
     1141 atacgcaggc ccagtttgcg ttggaacatc gcgccgcgat agtcgaacca ggtatagaac
     1201 gcgccttcgg gatggacttg gcgcaggctg tcggtcagtc ccaaatccag caggtttttg
     1261 aaccactgcc gttcgacgga cgaacagtgg attttttcgt gccatttttc agggtcgtaa
     1321 cagtccgcat cggcaggcgc gatattgaaa tcgcccagta gcaccagttt gccgtagcgg
     1381 gtcatttcat cgcggacaaa ttcggttaac gcggcaaacc attgttcttt atatttgaat
     1441 ttcgggctgt cgagggcttc gccgttgacg caatagacat tgatgacgcg cacaccgccg
     1501 acggttgccg caatcacgcg ccgttgcgga tcgtccggca gcgcgggcag cccgatatgc
     1561 acgtcttccg gcgcgttgcg gctgacgatt gccacgccgt tgtaggtttt ctgcccgctc
     1621 caaacacagt gccagcccat catttgcaaa gcggcggccg gaaatttgtc ctgatcgagt
     1681 ttgagttcct gcaaaaccaa aatatcgggc ggattgtcgg caagcaggtt ttgcacctgc
     1741 ggcagccgca cattgagcga attgacgttc caagtggtga ttttcataac atttccgaaa
     1801 aatgccgtct gaaacgttca gacggcaaaa ggaaaggatt ttaacactat accgcgcgtt
     1861 tgttttccaa atcgcggatt gtccgcagaa tcgttgccgc gtcttcggaa atcaggcggt
     1921 attcgataat gcggtgctaa cgcgtaaagt ccaccagacc ttccacgcgc agtcggctca
     1981 aatggttgga aaccgctgtc gcaggcatgg acagggattc ggacagctcg gcaatattgc
     2041 aatcgccgtc caacagttgg agcagtatcg ccatacgttc gggatgcgcc ataagtttga
     2101 gtatggtgtg gagcggtatt gtgttcatcg gtattcctaa aaccttttcc gcccgccaag
     2161 cggattgacg ggcggcagat atggatgatt gtgattatct ttataaatta tattataaat
     2221 caattattta tatatgattg tacaggattg tatgttcctt ttaccactat ttttgcaaac
     2281 acgcgtaaag gcgggctttc agacagcata aaactgccgc gcccgtcctt tcccctgccc
     2341 tgccgatgcc tccgcgctgt cgggcaaaca aaatacctgc ctgaaacgct gttttttcta
     2401 aagcatcacg accttcacga aaaatgccgt ctgaagaata tttcagacgg catttcctcc
     2461 ttgaggcaat aaccggatta cggttaggcg gttccttgtc tgacatatca gacaactgcc
     2521 gacaccctaa aactcatgcc aagaaatcca aacctatgtc caacgaacgg ctgctgtggg
     2581 tcagatagcc gagagcgatg ccgtccacgc cggtttgcgc cacgcgcttc agtcggtcga
     2641 agccgatgcc gcccgatgct tcgcaataga cggtgtgggg gtgggcggtt tgcgtgtggc
     2701 agcggtttgc cgcttctttc agggtttcgt cgtccatgtt gtccagcaaa atccgttccg
     2761 cgcccgctgc gatggcttcg tccagttgtg ccaacgtgtc cacttcgatt tctacacaag
     2821 tcaacggtcc gaccgcctgt tttgcctgcc gaacggcttg ggcgatgctg ccgcaatagg
     2881 cgaggtggtt gtctttgatg agcacggcgt cgtccaagcc catgcggtgg ttcacgccgc
     2941 cgcctgccct gacggcgtat ttttgcagga cgcgcagcag ggggatggtt ttgcggctgc
     3001 acacgatgtc tgtaccgtat tcggcgactt cggcaacggc acgcgcggtg gcggtggcga
     3061 tgccgcttaa gtgcgtgagg tagttgagcg cggtgcgttc ggcggcgagc agcgcgcggg
     3121 cgttgccttc gacggcggca agcgtctgac ctgcgcggac gacttgcccg tctcggattt
     3181 cggcttggaa gcgaacggac ggatccatcg tctgaaaggc gaggcgcgcc aagtccatgc
     3241 cggcgataac gccgtcttcg cggctgacga ggaagagttt ggcggttttt tcgggggcga
     3301 taacggcggc ggacgtaata tcgccgcgcc tgcccaaatc ttcgctcaag gcttgttcca
     3361 ccatggggcg caacagggtg tcgggcaggg ggaagagggt gttttcagac ggcatggtgt
     3421 ttcctttgtt gaaaaagatt gatattgccc gatggggcaa aacttcattt tatag
//
LOCUS       DAXHMX010000175         3474 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHMX010000175 DAXHMX010000000
VERSION     DAXHMX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.175"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            43..711
                     /locus_tag="ACFM4S_001953"
     CDS             43..711
                     /locus_tag="ACFM4S_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6051571.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTVNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTTDRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRNDRRTV
                     CQYK"
     gene            741..1355
                     /gene="pilV"
                     /locus_tag="ACFM4S_001954"
     CDS             741..1355
                     /gene="pilV"
                     /locus_tag="ACFM4S_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6051572.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSAVDGDFAVDA
                     VKSKKDLAKAQLDRFGYELKNALQDAAAIYYAVCKDSSGAAPTLSAGSTFSSNCDNKA
                     NGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            1352..2320
                     /locus_tag="ACFM4S_001955"
     CDS             1352..2320
                     /locus_tag="ACFM4S_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6051573.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKDVVSDVPQKNRL
                     FSLKGSSANKLIPNGTDNKLIPITESLDINYQNFFQVGSALIFQYGIDDVNASAATTV
                     VSSCAAISKPGKQILTLENVKKELKISDQDKEQNGNIARQRHVVNAYAVGRIAGEEGL
                     FRFQLDDKGKWGNPQLLAKKIRYMKVRYIYVSDCPEDDDAGKEETFRYTDKFNSAQDA
                     VTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2299..2892
                     /locus_tag="ACFM4S_001956"
     CDS             2299..2892
                     /locus_tag="ACFM4S_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6051574.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRFCPANSTDLCIDKKGMEYKKGKGSVSKPPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2897..3370
                     /locus_tag="ACFM4S_001957"
     CDS             2897..3370
                     /locus_tag="ACFM4S_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6051575.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIERGYQSQLYTE
                     MVGINNISKQFILKSPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCEAFSNRKK"
BASE COUNT          997 a          692 c          886 g          899 t
ORIGIN      
        1 aatttaatcc actatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacggtca acaataaatg tgactccggc
      301 aagaaggggc agggaatgtt ggctttcggc gacaaaaacg gcaataaggg atatgacaat
      361 gatacggcgg atgttctcct ccgcagtgtg gtattgaatg atgatatcaa tgataagcgg
      421 attaattatg ccttcaacca tatcgctttt ggtcagactc agccgaccac cgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga aagaccagca tcttacaaaa
      541 caatccagct ttttttattc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattgacag cagcggcagg
      661 gttgaggttt gtcctagaaa cgataggcgt accgtatgcc aatataaatg attgttttag
      721 tcgatttggg aaataaaaaa atgaagaata atgattgctt ccgcctgaaa aacccccagt
      781 ccggtatggc gctgatagaa gtcttggtcg ctatgctcgt tctgaccatc ggtattttgg
      841 cactattgtc tgttcagttg cggacagtcg cttccgtcag ggaggcagag acgcaaacca
      901 tcgtcagtca aatcacgcaa aacctgatgg agggaatgtt gatgaatccg accattgatt
      961 cggacagcaa caagaaaaac tataatcttt acatgggaaa ccatcataca ctatcagctg
     1021 tggatggcga ttttgcggtt gatgccgtga aaagcaagaa ggatttggca aaagcccagt
     1081 tggacaggtt cggttatgaa ttgaaaaatg ccttgcagga tgcggcagcc atctattacg
     1141 ccgtctgcaa ggattcgtcg ggtgctgcgc cgacattgtc cgccggcagt actttttctt
     1201 caaattgcga caataaggca aacggggata ctttgattaa agtattgtgg gtaaatgatt
     1261 cggcagggga ttcggatatc gcccgtacga atcttgagac gaacggcaac aatatcgtat
     1321 atacctatca ggcaagggtc ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa
     1381 aggcagttat gatggtatga agggttttac cattattgaa tttttggttg cgggcatgct
     1441 cagtatgatt gtcctgatgg cggtcggatc gagttacttc acgtcccgga aattaaatga
     1501 tgcggcaaac gagcgtcttg ctattcaaca ggatttgcgg aatgcggcaa cattaattgt
     1561 ccgcgatgca agaatggcgg ggagcttcgg ttgtttcaat atgtctgagc ataccgaaaa
     1621 ggatgttgtt tccgatgtgc cgcaaaaaaa ccgtcttttt tccttaaaag ggagcagtgc
     1681 aaataaactt atccccaacg gtacagataa taaacttatc cccataacgg aatctttaga
     1741 tatcaattat cagaattttt tccaggttgg tagcgcattg atttttcaat acggaatcga
     1801 tgatgttaat gcaagcgccg cgactaccgt cgtcagcagc tgtgccgcaa tatcgaaacc
     1861 gggcaagcaa atccttactt tagaaaatgt aaaaaaagaa ttgaagattt cggatcagga
     1921 taaggagcaa aatggcaata tagcgcgtca aaggcatgtg gtcaatgcct atgcggtcgg
     1981 caggattgcc ggtgaggaag gtttgttccg cttccaattg gatgataagg gcaagtgggg
     2041 taatcctcag ttgctcgcga aaaagattag atatatgaaa gtgcggtata tctatgtttc
     2101 cgactgtcct gaagatgacg atgccggcaa agaggaaaca ttcagatata cggataaatt
     2161 caacagcgcc caagatgctg ttacgcccgc cggggtggag gttttattga gtagcggtac
     2221 tgataccaag attgccgctt cttcagacaa tcatatttat gcttaccgta tcgatgcgac
     2281 aatacgcggg ggaaatgtat gcgcaaacag aacactttga cgggaatccc gacttctgac
     2341 ggacagaggg ggtttgcact gtttatcgtg ctgatggtga tgatcgtcgt ggcttttttg
     2401 gttgtaactg ccgcgcagtc ttacaatacc gagcagcgga tcagtgccaa cgaatcagac
     2461 aggaaattgg ctttgtcttt ggccgaggcg gctttgcggg aaggcgaact tcaggttttg
     2521 gatttggaat atgatacgga cagtaaggtt acatttagcg aaaactgtgg aaaaggtctg
     2581 tgtaccgcag tgaatgtgcg gacaaataat gataatgaag aggcttttga caatatcgtg
     2641 gtgcaaggca agcccaccgt tgaggcggtg aagcgttttt gccctgcaaa ttctaccgac
     2701 ctgtgcattg acaagaaagg gatggaatat aagaaaggca agggaagcgt cagcaaacca
     2761 ccgcgctata ttatcgaata tttgggcgtg aagaacggag aaaatgttta tcgggttact
     2821 gccaaggctt ggggtaagaa tgccaatacc gtggtcgtcc ttcaatctta tgtaagcaat
     2881 aatgatgagt aataaaatgg aacaaaaagg gtttacattg attgagatga tgatagtcgt
     2941 cgcgatactc ggcatcatca gcgtcattgc cataccttct tatcagagtt atattgaaag
     3001 aggctatcag tcccagcttt atacggagat ggtcggtatc aacaatattt ccaaacagtt
     3061 tattttgaaa agtcccctgg acgataatca gaccatcaag agcaaactgg aaatatttgt
     3121 ctcaggctat aagatgaatc cgaaaattgc cgaaaaatat aatgtttcgg tgcattttgt
     3181 caataaggaa aaaccaaggg catacagctt ggtcggcgtt ccaaagacgg ggacgggtta
     3241 tactttgtcg gtatggatga acagcgtggg cgacggatac aaatgccgtg atgccgcttc
     3301 tgcccgagcc catttggaga ccttgtcctc agatgtcggc tgtgaagcct tctctaatcg
     3361 taaaaaatag ggctgttttg ccgatgccgt ctgaaaatca atgttcagac ggcattttta
     3421 tgggtatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac agta
//
LOCUS       DAXHMX010000176         3433 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHMX010000176 DAXHMX010000000
VERSION     DAXHMX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.176"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..1458
                     /gene="cytX"
                     /locus_tag="ACFM4S_001958"
     CDS             235..1458
                     /gene="cytX"
                     /locus_tag="ACFM4S_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6051576.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVLV
                     RLFFKKTQSLQRNPS"
     gene            1455..2555
                     /locus_tag="ACFM4S_001959"
     CDS             1455..2555
                     /locus_tag="ACFM4S_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6051577.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVIKLGRQSIPLWRNIRGHLKTPAMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEGLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2576..3193
                     /gene="thiE"
                     /locus_tag="ACFM4S_001960"
     CDS             2576..3193
                     /gene="thiE"
                     /locus_tag="ACFM4S_001960"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6051578.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          695 a         1076 c          951 g          711 t
ORIGIN      
        1 aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac
       61 ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc
      181 cgttcccttt tccgcacttc cccgcccacc ttccttcttt ttaaaggacg tcatatgtcg
      241 ggcaatgcct cctctccttc atcttccgcc gccatcgggc tgatttggtt cggcgcggcg
      301 gtctcgattg ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcaacgcggt
      361 ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat
      421 atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc
      481 ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg
      541 atttacgccg gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct
      601 tttgtctggt gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca
      661 cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga tgctgttggc ggttctgtgg
      721 ctgagtgccg aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg
      781 agcttcggaa cggcagtcga attgtccgcc gtcatgccgc tttcctggct gccgcttgcc
      841 gccgactaca cgcgccacgc gcgccgcccg tttgcggcaa ccctgacggc aacgctcgcc
      901 tacacgctga ccggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga
      961 gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg
     1021 gtcgtcctct ccaccgttac cacaacgttt ctcgatgcgt actccgccgg cgcgagtgca
     1081 aacaatattt ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc
     1141 acggtgcttg ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc
     1201 tcggtatttg cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt
     1261 gaggagattg aaggctttga ctttgccgga ctggttctgt ggctggcggg cttcatcctc
     1321 taccgcttcc tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg
     1381 tctgccgttg ccattgccac cgtactggtg cgccttttct ttaaaaaaac ccaatcttta
     1441 caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga
     1501 ccgcactgca gcttgcagaa caaggttatc agattgcact tttcgataaa ggctgccgcc
     1561 ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg
     1621 tcgaagccac gcctgaagtc atcaaactgg gcaggcagag catcccgctt tggcgcaaca
     1681 tcagaggcca tctgaaaacg cctgccatga tgcaggaaaa cggcagcctg attgtgtggc
     1741 acgggcagga caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtgg
     1801 cggatgacga aatcgtccgt tggcgcgcag atgaaatcgc cgaacgcgaa ccgcaactcg
     1861 gcggacgttt ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa
     1921 tattgtctgc acttgccgac gctttggacg aactgaacgt cccctgccat tgggaacacg
     1981 aatgcgtccc cgaaggcctg caagcccaat acgactggct gatcgactgc cgcggctacg
     2041 gcgcaaaaac cgcgtggaac caatcccccg agcacaccag caccctgcgc ggcatacgcg
     2101 gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgtctgctcc
     2161 atccgcgtta tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga
     2221 cccaaatcga aagcgaaagc caagcccccg ccagcgtgcg ttcagggttg gaactcttgt
     2281 ccgcactcta tgccgtccac cccgccttcg gcgaagccga catcctcgaa atcgccaccg
     2341 gcctgcgccc cacgctcaac caccacaacc ccgaaatccg ttacaaccgc gcccgacgcc
     2401 tgattgaaat caacggcctt ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg
     2461 ccgccgtcag attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgtgatgaag
     2521 aaagcggttt ggcgtatatc cgaagacaag attaaagccg tctgaaagga accccatgac
     2581 cttcccgcca ctcaaatccc cgctcaaatt ctacgccgtc gtccccaccg ccgattgggt
     2641 ggggcgcatg gtcaaagcag gtgccgacac ggtgcaactg cgctgcaagg ccctgcacgg
     2701 cgatgaattg aaacgcgaaa tcgcccgctg tgtcgccgcc tgtcagggca gccatacgca
     2761 gcttttcatc aacgaccact ggcgcgaggc aatcgaagca ggcgcatacg gcgtgcatct
     2821 cgggcaggaa gatatggaca ccgccgacct cgccgccatt gccgccgccg gtttgcgctt
     2881 gggtttgagt acgcactccg tcgccgaact cgaccgcgcc ctgtccgtac accccagcta
     2941 tgtcgccagc ggcgcgattt tcccgaccac gaccaagcaa atgcccaccg ccccgcaagg
     3001 cttggacaaa ctgtgcgagt atgtgaaaca ggcaggcggc acgcccgtcg tcgccatcgg
     3061 cggtatcgac ttgaacaacg cccgagccgt actcgccacc ggcgtttcct cactcgccgc
     3121 cgtccgcgcc gtaaccgaag cggcaaatcc cgaagcggtg gttaaagcgt ttcaggcttt
     3181 gtgggatgga taaaaccgaa agaagaaaat tcaattgccg tgtcggcaaa acttagcccg
     3241 ttatcgcaaa catacttaac tttaaatgtg gcatatcatc aaattccgtc attcccgcgt
     3301 aagcgggaat ccgccttaaa acttgagaaa ccatcatttg aaaaacagtt tccgaatttc
     3361 aaaaatggat tcccgcccgt gcgggaatga cggcaaccgg tcagttgcgt atcaaaaaat
     3421 aaagtaattc ggc
//
LOCUS       DAXHMX010000177         3420 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHMX010000177 DAXHMX010000000
VERSION     DAXHMX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.177"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..330
                     /locus_tag="ACFM4S_001961"
     CDS             <1..330
                     /locus_tag="ACFM4S_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6051579.1"
                     /translation="RDGEDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFQK
                     SGIFQNSAQCPAPVTFTVTVLDSSRQFAFSFENACTIAERLRYMLLALAWAVAAFFCI
                     RTVSREV"
     gene            634..918
                     /locus_tag="ACFM4S_001962"
     CDS             634..918
                     /locus_tag="ACFM4S_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC6051580.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     SSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            919..2106
                     /locus_tag="ACFM4S_001963"
                     /pseudo
     CDS             919..2106
                     /locus_tag="ACFM4S_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin family protein"
     gene            2321..3277
                     /locus_tag="ACFM4S_001964"
     CDS             2321..3277
                     /locus_tag="ACFM4S_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC6051581.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDYNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         1123 a          736 c          774 g          787 t
ORIGIN      
        1 agggacggcg aagacggcgg ccttttgtgc aaattcttcc ccgacattct cgcttgcgac
       61 aggctgcccg agcccaatcc ggcagaagat ttaaatctgc cgtctgaaac cgtcaatgta
      121 gagtttcaga aatcaggaat ctttcaaaat tccgcacagt gtcccgcacc tgtcactttc
      181 acagtgactg tgcttgattc cagcaggcag ttcgcgttca gctttgagaa cgcatgtacc
      241 atagccgaac ggctaaggta catgcttctc gcccttgctt gggcggttgc cgcatttttc
      301 tgtatccgca cagtatcccg tgaagtctag caggcgcagc accgccgggc ttcagtaact
      361 tgtaccaagg caggaggagg acgtccagaa agatttgtaa agacggcttt atcgtcttta
      421 taaatctttt tggatacccc ttgccgcccc gccaaaagaa tacactctgc cgcaagggca
      481 ggtggtaagg cgcgcgcttt ttgcgccgtt ccccctgccc ccgcggcgtc gcaagtgaga
      541 ctgggggtgt gggggctagt ccccgcaaaa tctttcagat taagaaacat ttttttaatg
      601 aggcaaccgt gccttttaag aaagggatag caaatgaaat tgttggccgc attgattccg
      661 ctgttgatga gcgtcgcagg ccgtatattg actgcattag gcttgatggc tgttacttat
      721 tcgggtgtag atagattggt agcccatttt cagcaggcga taaccagtag cataacgggc
      781 gcgcctcaag cgatgttgca gctcttttat ataagcggtg gcggcaccgt tcttaatatc
      841 ctgtttggcg cgatcgcctt tattctgtca ttcaaacaaa tgacaaaact agcaacctca
      901 atcgggaaga aaaaataaat ggcagagatc tgtttgataa ccggcacgcc cggttcaggg
      961 aaaacattaa aaatggtttc catgatggcg aatgatgaaa tgtttaagcc tgatgaaaac
     1021 ggcatacgcc gtaaagtatt tacgaacata aaaggcttga aaataccgca cacctacata
     1081 gaaacggacg caaaaaagct gccgaaatcg acagatgagc agctatcggc gcatgatatg
     1141 tacgaatgga taaagaagcc cgaaaatatc gggtctattg tcattgtaga tgaagctcaa
     1201 gacgtatggc cggcacgctc ggcaggttca aaaatccctg aaaatgtcca atggctgaat
     1261 acgcacagac atcagggcat tgatatattt gttttgactc aaggtcctaa gcttctagat
     1321 caaaatctta gaacgcttgt acggaaacat taccacatcg cttcaaacaa gatgggtatg
     1381 cgtacgcttt tagaatggaa aatatgcgcg gacgatcccg taaaaatggc atcaagcgca
     1441 ttctccagta tctatacact ggataaaaaa gtttatgact tgtacgaatc agcggaagtt
     1501 cataccgtaa ataaggtcaa gcggtcaaag tggttttaca ctctgccagt aatagtattg
     1561 ctgattcccg tgtttgtcgg cctgtcctat aaaatgttga gcagttacgg aaaaaaacag
     1621 gaagaacccg cagcacaaga atcggcggca acagaacagc aggcagtact tccggataaa
     1681 acagaaggcg agccggtaaa taacggcaac cttaccgcag atatgtttgt tccgacattg
     1741 tccgaaaaac ccgaaagcaa gccgatttat aacggtgtaa ggcaggtaag aacctttgaa
     1801 tatatagcag gctgtataga aggcggaaga accggatgcg cctgctattc gcatcaaggg
     1861 acggcattga aagaagtgac ggagttgatg tgcaaggact atgtaaaaaa cggcttgccg
     1921 tttaacccat acaaagaaga aagccaaggg caggaagttc agcaaagcgc gcagcaacat
     1981 tcggacaggg cgcaagttgc cacattgggc ggaaaaccgt agcagaacct aatgtacgat
     2041 aattgggaag aacgcgggaa accgtttgaa ggaatcggcg ggggcgtggt cggatcggca
     2101 aactgaagaa aacggcaaga gagaaaaaag acccgtaaac cgtttgaata tagacggttt
     2161 acgggtcttt gtttcgcgca aagcaagggc taaggcagtc aggcagcaaa tcccgcaatg
     2221 tattaaaaca gacgcgtaga aatgccggct gcctttatcc atcctcaaaa ttgaatatca
     2281 tcctagccgt atcaaggctg tataaataag gaaaatacca atgaatataa tcgggctgga
     2341 catctcaaag gataccatag acgcaacatt gcataaaaca aacggaagta tccattacat
     2401 taaatttaag aataatgatg atggattaaa acagtttaga ttgtggataa agggaaacag
     2461 aatcagaaaa gcctatatcg gcatggaggc aacaggcatc tattacgaaa aggcagcaga
     2521 tatgctttct tcctactata ccgtttacgt tatcaatccc ttaaaaatca aggactacgg
     2581 gaaaagcagg tttaaccgta ccaaaaccga caaagcagat tcaaacctga tagcagatta
     2641 cataaaaagg catcaagata cattgatacc gtatcagata cccaaaaaca aagcactgca
     2701 aaaactgatt aaccttaaaa atcaattaca gcaacatcag aagcaaatta aaaaccgtct
     2761 tcatagcact gaagaagact tcataaggaa catacatcaa gacttgatag ataccataca
     2821 ggacaagatg gaacaggtaa aaatagccat atccgaacaa atcaaaaaac aaacggacta
     2881 taaccattac cgcaatcttc aaaccatccc gagcataggc aaagacaccg catcagttct
     2941 ttatgcgcaa ctgacagaaa aacattttaa aaccgcaaac cagtttgtat cctatgccgg
     3001 attaagtccc gccatcatac aatcagggac aagcgtaaga ggtcggggca gattgagccg
     3061 atacggaaac agacgattaa aaagtacgct gtatatgccc gccctttgtg cttaccgttt
     3121 taacgcattt ccgaaattaa taaataatct gaaaaaagcg ggtaagccaa agatggtaat
     3181 tatcgttgcc atcatgcgca aactggcgaa gctcgcctat tatattgtta aaaccggcca
     3241 gccttacgat gcggaaagac accgattgaa tcaataaaat tcaacaaaat taaacggtta
     3301 cgcgaatata tttgtgtaac cgtgcatttg catatcgtaa ataaacgtaa ataaaaataa
     3361 caatataaat cagtatattg caactttgtt ttttattttg tgttgacggg caacatatca
//
LOCUS       DAXHMX010000178         3343 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHMX010000178 DAXHMX010000000
VERSION     DAXHMX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.178"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            62..691
                     /locus_tag="ACFM4S_001965"
     CDS             62..691
                     /locus_tag="ACFM4S_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6051582.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            866..3241
                     /gene="hmbR"
                     /locus_tag="ACFM4S_001966"
     CDS             866..3241
                     /gene="hmbR"
                     /locus_tag="ACFM4S_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HFC6051583.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVLAADEAATETTPVKAEIKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYAVEGEGSGANIRGSARGIPDSSKHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLTASSWREADDVNRRRNANLFYEWMPDSNWLSSLKADFDYQKTKV
                     AAVNNKGSFPMDYSTWTRNYNQKDLDEIYNRSMDTRFKRFTLRLDSHPLQLGGGRHRL
                     SFKTFVSRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSSR
                     AGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWHVGYDITSG
                     YRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGMLDANLYQSNYRN
                     FLSEEQKLTTSGTPGCTEENAYYGICSDPYKEKLDWQMKNIDKARIRGIELTGRLNVD
                     KVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLTY
                     LGAKKAKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAGV
                     YNVFNRKYTTWDSLRGLYSYSTTNSVDRDGKGLDRYRAPSRNYAVSLEWKF"
BASE COUNT          909 a          859 c          834 g          741 t
ORIGIN      
        1 gcgggcaagt gcggattttg cccgcaccgc atccttatcc cttaccgaca aaggaaaaaa
       61 tatgagtgaa accgaaaatc aagcattgac atttgccaaa cgcttgaagg cggataccac
      121 ggcggttcac gacagcgtgg ataacctcgt tatgtctgtc caaccgtttg tcagcaaaga
      181 aaactacatc aaatttttga aactccaatc cgttttccac aaggctgtcg accacatcta
      241 taaagatgcc gaattaaaca aagccattcc cgagctggaa tacatggcgc gatacgatgc
      301 cgtaacgcaa gaccttgcag atttgggtga caaaccttac gaatacggca aaccgctgcc
      361 gcatgaaacc ggcaacaaag caatcggctg gctttattgc gccgaaggat ccaatttggg
      421 cgcggcgttt ttgttcaaac acgcccaaaa actcgattac aacggcgaac acggcgcgcg
      481 ccacctcgct ccgcatcccg acgggcgcgg caaacactgg cgtgccttcg tcgagcatct
      541 gaacgctttg aacctgactc ccgaagccga agcggaagcc atccaaggcg cgcgcgaagc
      601 ctttgcattc tacaaagtcg tgttgcgcga aaccttcggt ttggcagccg atgccgaagc
      661 accggaagga atgatgccgc acaggcacta aaaaataatc gaaccaaata aacaaggtct
      721 cggcatagct gtttgcaggg acctttaatt acacggcgcg gctttgttta catggattac
      781 tgtcttatta aatattaatg attatcataa aatctattat tcgctaaccg atggatgaac
      841 aatccataca tcttgagttg ataatatgaa accattacaa atgctcccta tcgccgcgct
      901 ggtcggcagt attttcggca atccggtctt ggcagcagat gaagctgcaa ctgaaaccac
      961 acccgttaag gcagagataa aagcagtgcg cgttaaaggt cagcgcaatg cgcctgcggc
     1021 tgtggaacgc gtcaacctta accgtatcaa acaagaaatg atacgcgaca ataaagactt
     1081 ggtgcgctat tccaccgatg tcggcttgag cgacagcggc cgccatcaaa aaggctttgc
     1141 tgttcgcggc gtggaaggca accgtgtcgg cgtgagcata gacggtgtaa acctgcctga
     1201 ttctgaagaa aactcgctgt acgcccgtta tggcaacttc aacagctcgc gtttgtctat
     1261 cgaccccgaa ctcgtgcgca acatcgaaat cgtgaagggc gcagactctt tcaataccgg
     1321 cagtggtgca ttgggcggcg gtgtgaatta ccaaacgctg caaggccgtg atttgctgtt
     1381 ggacgacagg caattcggcg tgatgatgaa aaacggttac agcacgcgta accgtgaatg
     1441 gacaaatact ctcggtttcg gtgtgagtaa cgaccgcgtg gatgctgctt tgctgtattc
     1501 gcaacgtcgc ggtcatgaaa ccgaaagcgc gggaaaccga ggctatgctg tggaagggga
     1561 aggcagtggc gcgaatatcc gtggttcggc acgcggtatc cctgattcgt ccaaacacaa
     1621 ataccacagc tttttgggta agattgctta ccaaattaac gataaccacc gcatcggcgc
     1681 atcgcttaac ggccagcagg gacataatta cacggttgaa gagtcttata acctgaccgc
     1741 ttcttcctgg cgcgaagccg atgacgtaaa cagacggcgc aatgccaacc tcttttacga
     1801 atggatgcct gattcaaatt ggttgtcgtc tttgaaggcg gacttcgatt atcagaaaac
     1861 caaagtggcg gcggttaaca acaaaggctc gttcccgatg gattattcca cctggacgcg
     1921 caactataat cagaaggatt tggacgaaat atacaaccgc agcatggaca cccgattcaa
     1981 acgttttact ttgcgtttgg acagccatcc gttgcaactc gggggggggc gacaccgcct
     2041 gtcgtttaaa actttcgtca gccgccgtga ttttgaaaac ctaaaccgcg acgattatta
     2101 cttcagcggc cgtgttgttc gaaccaccag cagtatccag catccggtga aaaccaccaa
     2161 ctacggtttc tcactgtctg accaaattca atggaacgac gtgttcagta gccgcgcagg
     2221 tatccgttac gaccacacca aaatgacgcc tcaggaattg aatgccgagt gtcatgcttg
     2281 tgacaaaaca ccacctgcag ccaacactta taaaggctgg agcggttttg tcggcttggc
     2341 ggcgcaactg aatcaggctt ggcatgtcgg ttacgacatt acttccggct accgcgtccc
     2401 caatgcgtcc gaagtgtatt tcacctacaa ccacggttcg ggtaattggc tgcctaatcc
     2461 caacctgaaa gccgagcgca gcaccaccca caccctgtct ctgcaaggcc gcagcgaaaa
     2521 aggcatgctg gatgccaacc tgtatcaaag caattaccgc aatttcctgt ctgaagagca
     2581 gaagctgacc accagcggca ctcccggctg tactgaggaa aatgcttact acggtatatg
     2641 cagcgacccc tacaaagaaa aactggattg gcagatgaaa aatatcgaca aggccagaat
     2701 ccgcggtatc gagctgacag gccgtctgaa tgtggacaaa gtagcgtctt ttgttcctga
     2761 gggttggaaa ctgttcggct cgctgggtta tgcgaaaagc aaactgtcgg gcgacaacag
     2821 cctgctgtcc acacagccgc tgaaagtgat tgccggtatc gactatgaaa gtccgagcga
     2881 aaaatggggc gtattctccc gcctgaccta tctgggcgcg aaaaaggcca aagatgcgca
     2941 gtacaccgtt tatgaaaaca agggctgggg tacgcctttg cagaaaaagg taaaagatta
     3001 cccgtggctg aacaagtcgg cttatgtgtt tgatatgtac ggcttctaca aaccggctaa
     3061 aaacctgact ttgcgtgcag gcgtatataa tgtgttcaac cgcaaataca ccacttggga
     3121 ttccctgcgc ggcctgtata gctacagcac caccaactcg gtcgaccgcg atggcaaagg
     3181 cttagaccgc taccgcgccc caagccgtaa ttacgccgta tcgctggaat ggaagtttta
     3241 atctggtatt attgaattaa tcgccttgtt gaaaattaaa gccgtccgaa ttgtgttcaa
     3301 gaactcattc ggacggcttt ttaccgtaaa tctgtgtgat ggg
//
LOCUS       DAXHMX010000179         3320 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHMX010000179 DAXHMX010000000
VERSION     DAXHMX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.179"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..2145)
                     /locus_tag="ACFM4S_001967"
     rRNA            complement(<1..2145)
                     /locus_tag="ACFM4S_001967"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(2554..2629)
                     /locus_tag="ACFM4S_001968"
     tRNA            complement(2554..2629)
                     /locus_tag="ACFM4S_001968"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2594..2596),aa:Ala,seq:tgc)
     gene            complement(2635..2711)
                     /locus_tag="ACFM4S_001969"
     tRNA            complement(2635..2711)
                     /locus_tag="ACFM4S_001969"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2675..2677),aa:Ile,seq:gat)
     gene            complement(2813..>3320)
                     /locus_tag="ACFM4S_001970"
     rRNA            complement(2813..>3320)
                     /locus_tag="ACFM4S_001970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          697 a          946 c          705 g          972 t
ORIGIN      
        1 ccacagagac tggcgtctct gcttcaaagc ctcccaccta tcctacacaa gtgacttcaa
       61 agtccaatgc aaagctacag taaaggttca cggggtcttt ccgtctagca gcgggtagat
      121 tgcatcttca caaccacttc aacttcgctg agtctcagga ggagacagtg tggccatcgt
      181 tacgccattc gtgcgggtcg gaacttaccc gacaaggaat ttcgctacct taggaccgtt
      241 atagttacgg ccgccgttta ctggggcttc gatccgatgc tctcacatct tcaattaacc
      301 ttccagcacc gggcaggcgt cacaccctat acgtccactt tcgtgttagc agagtgctgt
      361 gtttttaata aacagtcgca gccacctatt ctctgcgacc ctccggggct tacggagcaa
      421 gtccttaacc ttagagggca taccttctcc cgaagttacg gtatcaattt gccgagttcc
      481 ttctcctgag ttctctcaag cgccttagaa ttctcatcct gcccacctgt gtcggtttgc
      541 ggtacggttc gattcaaact gaagcttagt ggcttttcct ggaagcgtgg tatcggttac
      601 ttcgtgtccg tagacactcg tcgtcacttc tcggtgttaa gaagacccgg atttgcctaa
      661 gtcttccacc taccggctta aacaagctat tccaacagct tgccaaccta accttctccg
      721 tccccacatc gcatttgaat caagtacagg aatattaacc tgtttcccat cgactacgca
      781 tttctgcctc gccttagggg ccgactcacc ctacgccgat gaacgttgcg caggaaacct
      841 tgggctttcg gcgagcgggc ttttcacccg ctttatcgct actcatgtca acattcgcac
      901 ttctgatacc tccagcacac tttacaatgc accttcatcg gcctacagaa cgctccccta
      961 ccatgccggt aaaccggcat ccgcagcttc ggttatagat ttgagccccg ttacatcttc
     1021 cgcgcaggac gactcgacca gtgagctatt acgctttctt taaatgatgg ctgcttctaa
     1081 gccaacatcc tggctgtctg ggccttccca cttcgtttac cacttaatct atcattgggg
     1141 accttagctg gcggtctggg ttgtttccct cttgacaacg gacgttagca cccgctgtct
     1201 gtctcccgag gaaccacttg atggtattct gagtttgcca tgggttggta agttgcaata
     1261 accccctagc cataacagtg ctttaccccc atcagtgtct tgctcgaggc actacctaaa
     1321 tagttttcgg ggagaaccag ctatctccga gtttgtttag cctttcaccc ctatccacag
     1381 ctcatccccg cattttgcaa catgcgtggg ttcggtcctc cagtacctgt tacggcacct
     1441 tcaacctggc catggataga tcactcggtt tcgggtctac acccagcaac tcatcgccct
     1501 attaagactc ggtttcccta cgcctcccct attcggttaa gctcgctact gaatgtaagt
     1561 cgttgaccca ttatacaaaa ggtacgcagt cacacctcta gggtgctccc actgtttgta
     1621 tgcatcaggt ttcaggttct gtttcactcc cctcccgggg ttcttttcgc ctttccctca
     1681 cggtactggt tcactatcgg tcgatgatga gtatttagcc ttggaggatg gtccccccat
     1741 attcagacag gatttcacgt gtcccgccct acttttcgta cgcttagtac cgctgttgag
     1801 atttcgaata cgggactgtc acccactatg gtcaagcttc ccagcttgtt cttctatctc
     1861 gacagttatt gcgtacaggc tcctccgcgt tcgctcgcca ctacttgcgg aatctcggtt
     1921 gatttctttt cctccgggta cttagatggt tcagttctcc gggttcgctt ctctaagtct
     1981 atgtattcaa cttaggatac tgcacagaat gcagtgggtt tccccattcg gacatcgcgg
     2041 gatcattgct ttattgccag ctcccccgcg cttttcgcag gcttacacgt ccttcgtcgc
     2101 ctatcatcgc caaggcatcc acctgatgca cttattcact tgactctatc atttcaagaa
     2161 cttctttgac ttcgtttgcc tacctgttga ctaagtaaaa gcaaacttga aattcctact
     2221 ttgataaagc ttactgcttt gttgtgtctt aatcctgcct tttgtgtttc aggattaagt
     2281 cgatacaatc atcacccaaa tactgtgttt gttttctttt ctcttgtgag cgatttttta
     2341 tcctttgcaa agaataaaaa atcaaaacaa acgctttgtc tttgtttgtt gatttcggct
     2401 ttccaatttg ttaaagatcg atgcgttcga tattgctatc tactgtgcaa atcaaaacga
     2461 gctgattatt atatcagcat tttgttcttg gtcaagtgtg atgtcgccct gaatggattc
     2521 tgttccattc ttccgttttg atttgtacag tattggtgga ggcaaacggg atcgaaccga
     2581 tgaccccctg cttgcaaagc aggtgctcta ccaactgagc tatgcccccg ttattggtgg
     2641 gtctgggagg acttgaacct ccgaccccac gcttatcaag cgtgtgctct aaccagctga
     2701 gctacaaacc cggattctct tcttaagcga atcttgcctt cactcaagct tcttccgcat
     2761 ctttttcagt ttaccgataa gtgtgaatgc ctaaagcctc ttctttctct agaaaggagg
     2821 tgatccagcc gcaggttccc ctacggctac cttgttacga cttcacccca gtcatgaagc
     2881 ataccgtggt aagcggactc cttgcggtta tcctacctac ttctggtatc ccccactccc
     2941 atggtgtgac gggcggtgtg tacaagaccc gggaacgtat tcaccgcagt atgctgacct
     3001 gcgattacta gcgattccga cttcatgcac tcgagttgca gagtgcaatc cggactacga
     3061 tcggttttgt gagattggct ccacctcgcg gcttggctac cctctgtacc gaccattgta
     3121 tgacgtgtga agccctggtc ataagggcca tgaggacttg acgtcatccc caccttcctc
     3181 cggcttgtca ccggcagtct cattagagtg cccaactgaa tgatggcaac taatgacaag
     3241 ggttgcgctc gttgcgggac ttaacccaac atctcacgac acgagctgac gacagccatg
     3301 cagcacctgt gttacgactc
//
LOCUS       DAXHMX010000180         3312 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHMX010000180 DAXHMX010000000
VERSION     DAXHMX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.180"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            101..1183
                     /locus_tag="ACFM4S_001971"
                     /pseudo
     CDS             101..1183
                     /locus_tag="ACFM4S_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
     gene            complement(1353..1562)
                     /locus_tag="ACFM4S_001972"
     CDS             complement(1353..1562)
                     /locus_tag="ACFM4S_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051584.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1559..1855)
                     /locus_tag="ACFM4S_001973"
     CDS             complement(1559..1855)
                     /locus_tag="ACFM4S_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC6051585.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1834..2439)
                     /locus_tag="ACFM4S_001974"
     CDS             complement(1834..2439)
                     /locus_tag="ACFM4S_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051586.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            2519..2806
                     /locus_tag="ACFM4S_001975"
     CDS             2519..2806
                     /locus_tag="ACFM4S_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC6051587.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2778..3080
                     /locus_tag="ACFM4S_001976"
     CDS             2778..3080
                     /locus_tag="ACFM4S_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC6051588.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIVEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
BASE COUNT          704 a          903 c          879 g          826 t
ORIGIN      
        1 cacgcaatgt accctgaccg gcaaaacttc cccaaacttc atcaaaaaat caactgtacc
       61 cgtctttttc agacggcatt gataccgtaa gaaatttccc atgcagaaat ggcaaaccga
      121 gctttattcc acaccgcttt ggctttgcct gacaccgaaa agcacggtca aagtcttgct
      181 gctgattacg gtaatggtta cgctgctgtt gaccgaagta cggctgaatg tgttgagtac
      241 ctttatgtcc aaaggacttt acgactcgat gcaggatttg aacgcctccg cgttttggat
      301 gtttgccgcg atgaacgcgg gcgtggtgct gatacgggcg tttaacaatg tcgtcaacga
      361 cttcctcgat caaggcttgg cgattaaatg gtcggagcag ctcaatgaag tgctgacgac
      421 gcgctggctt gccgacaaaa actactaccg cctgcaaatg cgccgccacg cgccggacaa
      481 catcgaccaa cgtatccaac aggacgcgca ggatttcatc gcttcgacca tcgaatttgt
      541 acgcggcatg gtcaattcgg tcgttacctc tttggaattt gccgttgttt tgtggggctt
      601 ggctggcatc ctgaccgtgt tcggcttcga cattccgcgc ggcattgttt ggctcgtctt
      661 tatttttgtg attttggcga cctttatcgc gatgtggata ggcaacccct tgattcgtta
      721 caactatgaa aacgaaaaac tcaacggcga ctaccgttat tccctcatcc gcgtacgcga
      781 ccacgccgaa agcgtagcgt tttacaacgg cgaacaacac gaacacggcc ggctttccga
      841 ccgctttaaa gccattatcc gcaaccgttg gcgcatcgcg cggcaaagcg tctgcttaag
      901 cggctttaac gatatgttca ccaacggcat caaactcttc cccattattt tgcaagcgcc
      961 gcgcctgttt gccggacaaa tcaaaatcgg cgacatccag cagaccgtcc aagcctttgc
     1021 gcgactgcaa aacgcgctat cttttttccg gatgttctac aacaaattca ccgcctaccg
     1081 cgcccgattg gagcgtctgt acggcttttt gctgagtacc gaagaacaac acagcgcgcg
     1141 gcaacccgag attagcgaag tttcagacgg cattgcactg gaatgtaagg cggcggcagc
     1201 ttttgcacga attggcggca gcttttgcac cgccgaagcg taggaaattg tattagcttt
     1261 tgcacaaatt tatccccgtt taatggtctg ttaaacgggg attatcttat gcctttaaaa
     1321 gtagtacgct acttgcggtc agaacttata aatcaggtgg tcgtcgtagc ttgaattgct
     1381 gttgatgtgc tgctcacagt caaggtttgg ctgcctgcaa aacgctccca tccggtcagg
     1441 cgcagttctg ccacgaggtc aagataagta ggcagttcgc tgcgggtgcc ggagaagaag
     1501 acaaatggcg gccgcaccat tgccatcagg cgcagaaact ctaccatgcc gaagtagttc
     1561 atcgcgtccc cttttccccg gcggcgcggc tttggttgaa ccgcgcgtcg tgctgcctgc
     1621 ctttcagttc gctgatatac gcctgccggc agtgttggcg gtcgaaaaat acgccgttga
     1681 tcagggtgta taaaaccttc cagcgggttt tgggcttttg ggcgagccgc gcgccgcgat
     1741 aggtgcggct tgagagggtt tcgtccgccg cgccgccggt tagggcgttg aacagttggt
     1801 cgatggctac caacaggtgg taaacgtatt ttttcagccg atgtttagcg tccattcttc
     1861 gatttccttt tccagcgcgt ccaatcctgg cgcggtttcg atggtgttca atttgtcttc
     1921 gagctgctgc cgctttccga taatcgcgcc ggcggcaacg gccaggcggg cggatttttc
     1981 gacaactttt tcaatcaaaa cgtccaattc cacgcccctt gcggcggcga tttgcgccag
     2041 catcggggtc ggggcgttgt tgtccgcctg ccgcgcgagg gcttcttttt cctgcctgta
     2101 aaagctgtcg atttccactt ggggatagcc cgccaagagg ctgtttttga gttcgtccgc
     2161 ctttgccgcg aggcggaatg ccgtggcggt tttttgttcg gcgaaacggg cggcggcagc
     2221 ggcttcgccg atttcccatt tcttgccgtc ccattcgtgg tattcggacg ggcgcggcgg
     2281 ggttaaaacg gggcggccgt cggaatctgc ggcaatctgc ccgccctgcg cctgtcctgc
     2341 caaaagggcg gcgtattctt cggcgcggac ggcaaccgcg ccttcgggga tgctgcccaa
     2401 ggtgtcgtcg taaaagccgt ttttgaaata gatggtcatt tcggggtttc cttttttgta
     2461 tttacatttt ggttttttgc cgcttataat gtaacgacaa aatacgctgc aagctgcaat
     2521 gaagattgaa tttgactccg aaaaaaatca acgcaatatc gaagagcgga atttgccctt
     2581 tgaatcagtc gggcaaatac gctggacaac ggccgttatt gtgcccgatg tctgctttga
     2641 ttaccccgaa ccgcgttatg ttgccgctgc ttatctaggc gatacgcaac gcctgcacat
     2701 tgtctgtttt acgccgataa aagacggcat ccgtgtcatc agttttcgca aagcgaataa
     2761 aagagaggtt aaaaaatatg caacgtcaat ccttaacaaa cgctgacggc gaagtgcggg
     2821 aacttgccgc cgaagatttt gcgcttgccc gccccattgt cgaagccctg cccgaagact
     2881 tggcacaggt gcttttttcc catcaaaaac aattggaaga aaagggcgta atgcaaaaac
     2941 gaaacacagg caaaaccccg aaacaactcg ttaccatacg cctgtccgcc gatgtggtcg
     3001 aaaaattccg cgccggcggc aagggctggc agacgcgcat caacgaggtt ttgcgccaat
     3061 atgtcgccca attgaaataa cgggggcggc cgctgcttat cgggcgcgcc cgactatcct
     3121 ttcccgcgcg gtttcggccg tgcggttttt tgttgccggt gccgtccgaa gggggcagcg
     3181 gctttttgcg cggtcattcc cgcctttcgt tattcccgcc actttccgtc attcccgcga
     3241 aggcgggaat cccgaaaccc ctgccgccgt cattcccgcg caggcgggaa tccagtccgt
     3301 tcggtttcag cc
//
LOCUS       DAXHMX010000181         3258 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHMX010000181 DAXHMX010000000
VERSION     DAXHMX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.181"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="ACFM4S_001977"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="ACFM4S_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6051589.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIADLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDTIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKESLLFGAE"
     gene            2186..3070
                     /locus_tag="ACFM4S_001978"
     CDS             2186..3070
                     /locus_tag="ACFM4S_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051590.1"
                     /translation="MTSIILELQSLAANPNSDVEELVSKTLMVARKLKINELTEWCNN
                     ELQGYSSKNLPKYRIFYGELKAFNPYRGDVPFMLPDELRERVTRVEYTSSISEIRNLL
                     KTKNNNFIRILSSKEKQFLMNLQDTPFPLEPKIIIYPINLMNIQSKVRQLILAWALRL
                     EEEGILGDGIQFTKSKQEKAMSNQFNIQNMQGFIGDIFGGNVQQNIQDGIHIEKENFN
                     KLANFLAKNGIPSAELEDLRNALIEDGQPTDKKFGSKVSAWIGNIITKASTGAIDVSV
                     GTIAGILTNAISKYYGLM"
BASE COUNT         1001 a          858 c          671 g          728 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt cattcgatac cccgattccc ccttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcctaccgct attgccgact tgctcgaagg gctttcagac ggccttgcct
      241 accaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 atgccgaaat gcgcgagttt ttccctgaaa acgcggtgga atatttcgtc tcctactacg
      421 actattacca acccgaagcc tatgtgccaa gccgcgattt gttcatcgaa aaagacagtg
      481 cgatcaacga acacattgaa caaatgcgcc tttctgccac caaaaacctg atgacgcgcg
      541 atgacgtgat tatcgttgcc actgtgtccg ccatttacgg tatcggcgac ccgactgagt
      601 atcaacaaat ggtgttgtcc gtcaaagaag gcgacaccat cgagcagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgaacgcg gcgatttgga tttcaaacgc ggcagcttcc
      721 gcgtgcgcgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgta
      781 tcagcctgtt tgacgacgaa atcgaccgcc tcgatatgtt tgacccgctt tcaggcagcc
      841 tgcaccaacg cgtcggccgc tacaccgtct tcccgtccag ccactacgtt accccgcgcg
      901 acaccgtatt gcgcgcctgc gagtccatca aagaagaatt gcgcgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgagcaac aacgcatcga acagcgcacc cgtttcgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggtatcga aaactactcc cgtcacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgccc gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacacaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgatt acggcttccg cctaccttcc gcccgcgaca
     1261 accgcccgct caaattccac gaatttgaaa aagtcatgcc gcaaaccgtc ttcgtttccg
     1321 ccacccccgc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggct ggtcgaccct caaatcatca tccgccccgt cgccacccaa gttgatgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgtactcgtt accaccctta
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacatcgac accgtagagc gcgttgaaat tattagagat ttacggctcg
     1621 gattgtttga cgtactcgtc ggcatcaatc tcttgcgcga aggcctcgac atcccagaag
     1681 tctccctcgt cgccatcctc gacgcagaca aagaaggctt cctgcgctcc caccgcagcc
     1741 tgattcaaac cataggccgc gctgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcaaca gattaaaaaa caggtcaaag
     1921 acatcattga cggcgtgtac cacgaagaag acagcggcaa aggccgtaga caaggcaaaa
     1981 acaaagttaa agtcggcgaa attcacaacg aagaagacac gattaaagaa atcgccaaac
     2041 tcgaaaaagc catgcaacag gcggctaggg atttgcagtt tgaagaagcg gctgttcttc
     2101 gtgataggat acggaatatt aaagagagtt tgttgtttgg ggcggaataa ttttattatt
     2161 tttatttaac atggagatta attaaatgac ttctattatt ttagaactcc aaagcctagc
     2221 tgccaatcca aatagtgatg tagaagagtt agtgagtaaa acattaatgg ttgcaagaaa
     2281 attaaaaatt aatgaattaa ctgaatggtg taataatgaa ttacaaggtt attcatcaaa
     2341 aaatttacca aaatatagaa tattttatgg agagttaaaa gcttttaacc cttatcgtgg
     2401 cgatgttcca tttatgcttc ccgatgaatt gagagaacga gtaacaagag ttgaatatac
     2461 ctcatcaatt agcgaaataa gaaacttact aaaaacaaaa aataataatt ttatacgaat
     2521 tctatctagt aaagaaaaac agtttttaat gaatttacaa gatactccat ttccacttga
     2581 acctaaaata attatttatc caattaactt aatgaatatt caatcaaaag taagacaact
     2641 aattttagct tgggcattac gactggaaga ggaaggtatt ttaggagacg gtatccaatt
     2701 tacaaaatca aaacaggaga aagcaatgtc aaaccaattc aatattcaga atatgcaagg
     2761 gtttattggg gatatttttg gcggtaatgt tcagcaaaat attcaagacg gtattcatat
     2821 tgaaaaagaa aatttcaata aactggctaa ctttttagca aaaaacggga ttccatctgc
     2881 cgaattagaa gatttacgaa atgcactgat agaagatggt cagcccacag ataaaaaatt
     2941 cggctcaaaa gtaagtgcat ggattggcaa tattattaca aaggcttcta ccggtgcaat
     3001 tgatgtttca gtaggtacta ttgcaggaat attaactaat gcgatttcaa aatattatgg
     3061 cttaatgtaa gcaaatgcaa tatgtgcggc tccaccacac acgccgtccc caaccgccca
     3121 aaaccgcgtg cctgcctgcg gcttaccccc taccacccaa ccaacacagc aagcgtaggt
     3181 tggatacttg tatccgacaa aacctttaac attcccatca ttgcaatcca ttgtagcaat
     3241 gcccaaaatg tcggattc
//
LOCUS       DAXHMX010000182         3247 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHMX010000182 DAXHMX010000000
VERSION     DAXHMX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.182"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>3247
                     /locus_tag="ACFM4S_001979"
     CDS             <1..>3247
                     /locus_tag="ACFM4S_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6051591.1"
                     /translation="YITGKEKGVLAAQAGKDINIIAGQISNQSEQGQTRLQAGRDINL
                     DTVQTGKHQATHFDADNHVIRGSTNEVGSSIQTKGDVTLLSGNNLNAKAAEVSSANGT
                     LAVSAKNDINISAGINTTHVDDASKHTGRSGGGNKLIITDKAQSHNETAQSSTFDGRQ
                     VVLQAGNDANILGSNVISDNGTRIQAGNHVRIGTTQTQSQSETYHQTQKSGLMSAGIG
                     FTIGSKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQIGSTVSSPEGNNIIHAQ
                     SIDIQAAHNKLNSNTTQTYEQKGLTVAFSSPVTDLAQQAIAVAQSSKQVGQSKNGRVN
                     AMAAANAGWQAYQTGKSAQNLANGTTNAKQVSISITYGEQQNRQTTQVQASQAQASQI
                     QAGGKTTLIATGAAEQSNINIAGSDIAGKAGTILIADNDITLQSAEQSNTERSQNKST
                     GWNAGAAVSFGQGGWSLGVTAGGNVGKGYGNGDSVTHRHSHIGDKGSQTLIQSGGDTT
                     IKGAQVRGKGVQVNAKNLSIQSVQDRETYQSKQQNASAQVTVGYGFSASGDYSQSKIR
                     ADHASVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSSQSAEDKGKNRFQTATLTTSDI
                     QNYSQYQGKSFGLGASVAVSGKTLGQGAQNKPQNKHLTSVADKNGASSSVGYGSDSDS
                     QSSITKSGINTRNIHITDEAGQIRLTGKTAAQTKADIDTNVTTDTAERHSGSLKNTFN
                     KEAVQSELDLQREVTQEFDKTRQGVKQELYAVVDSKREQATKERLKNGGYDNDKSRAL
                     NKEANELDEKIRWLDAGLGLVWGAGSSDMAWSMFATTQADRAVRSATAPKEMWFHKKI
                     IDEKTGKVSFDTRQIWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQ
                     NRNSTNGSGVIAVMNPPTGKYKSDSNNKIKDSLWLSSSLVSELMYVGYDQLNNKVFQG
                     YLPKTNSEKLNQDIYREVQKMDNGWSVDTSNHSRGGITASVSLKDWVNNKEQNGIAPI
                     RKARFYGTATNVKNDYADVLQKNGYTYTGADGKTYNSGAYSIVHDKDFVGNK"
BASE COUNT         1173 a          759 c          718 g          597 t
ORIGIN      
        1 tatatcacag gcaaagaaaa aggtgtttta gcagcgcagg caggcaaaga catcaacatc
       61 attgccggtc aaatcagcaa tcaatcagag caagggcaaa cccggctgca agcagggcgc
      121 gacattaacc tagatacggt acaaaccggc aaacatcaag caacccattt tgatgccgat
      181 aaccatgtta ttcgcggttc aacgaacgaa gtcggcagca gcattcaaac aaaaggcgat
      241 gttaccctat tgtcagggaa taacctcaat gccaaagctg ccgaagtcag cagcgcaaac
      301 ggtacactcg ctgtgtctgc caaaaatgac atcaacatca gcgcaggcat caacacgacc
      361 catgttgatg atgcgtccaa acacacaggc agaagcggtg gtggcaataa attgattatt
      421 accgacaaag cccagagcca caacgaaacc gctcaaagca gcacctttga cggcaggcaa
      481 gttgtattgc aggcaggaaa cgatgccaac atccttggca gcaatgttat ttccgataat
      541 ggcacccgga ttcaagcagg caatcatgtt cgcattggta caacccaaac tcaaagccaa
      601 agcgaaacct atcatcaaac ccagaaatca ggattgatga gtgcaggtat cggcttcact
      661 attggcagca agacaaacac acaagaaaac caatcccaaa gcaacgaaca tacaggcagt
      721 accgtaggca gcttgaaagg cgataccacc attgttgcag gcaaacacta cgaacaaatc
      781 ggcagtaccg tttccagccc agaaggcaac aatatcatcc atgcccaaag catagacatt
      841 caagcggcac acaacaaatt aaacagtaat accacccaaa cctatgaaca aaaaggctta
      901 acggtggcat tcagttcgcc cgttaccgat ttggcacaac aagcgattgc cgtcgcacaa
      961 agcagcaaac aagtcggaca aagcaaaaat ggtcgtgtca atgccatggc agctgccaat
     1021 gcaggctggc aagcctatca aacaggtaag agtgcacaaa acttagccaa tggtacaacc
     1081 aatgccaaac aagtcagcat ctccataacc tacggcgaac agcaaaaccg acaaaccacc
     1141 caagttcaag caagccaagc ccaagcgagt caaattcaag caggcggtaa aaccacatta
     1201 atcgccacag gcgcagcaga acaatccaat atcaacatcg caggctcaga tattgccggc
     1261 aaagcaggca caatcctgat tgccgataac gacatcacac tccaatcagc cgagcaaagc
     1321 aataccgaac gcagccaaaa caaatcgaca ggttggaacg caggtgctgc cgtatcattc
     1381 ggacaaggag gctggtcatt aggcgttacc gcaggcggca atgtcggcaa aggctacggc
     1441 aatggcgata gcgtaaccca ccgccatagc catattggcg acaaaggcag ccaaaccctt
     1501 atccaaagcg gtggcgacac taccatcaaa ggcgcgcaag tacgcggcaa aggcgtacaa
     1561 gtcaatgcca aaaacctaag tattcaaagc gtacaagata gagaaactta tcaaagcaaa
     1621 caacaaaacg ccagtgcaca agtgaccgta ggttatggct tcagtgccag tggcgattac
     1681 agtcaaagca aaatccgagc cgaccatgct tcagtaaccg agcaaagcgg tatttatgcc
     1741 ggagaagacg gctatcaaat caaggtcgga aaccatacag acctcaaagg cggcatcatc
     1801 accagcagcc aaagcgcaga agacaagggt aaaaaccgct ttcagacggc cacccttact
     1861 accagcgaca ttcaaaatta cagccaatac caaggaaaaa gttttggatt gggtgccagc
     1921 gttgccgtaa gcggcaaaac actgggacag ggcgcacaaa ataaacctca aaacaaacac
     1981 ctgacaagcg tagccgataa aaacggcgca agttcatcag tgggttatgg cagcgacagc
     2041 gacagtcaaa gcagcatcac gaaaagcggc atcaacaccc gcaacataca catcaccgac
     2101 gaagcgggac aaatcaggct gacaggcaaa acagcggcac aaaccaaagc cgatattgat
     2161 acaaacgtaa ccacagacac cgccgaacga cattcgggca gcttgaagaa caccttcaac
     2221 aaagaagcgg tgcaaagtga actggattta caaagggaag taacccaaga gtttgataaa
     2281 actagacaag gagtgaaaca agaactttat gcagtagtag atagcaaacg agagcaagcc
     2341 acaaaagaga gactcaaaaa tggtggatat gacaatgata aatctagagc attaaataag
     2401 gaagctaatg aattagatga aaaaattcgt tggcttgatg cgggattagg actcgtttgg
     2461 ggagcaggga gttctgatat ggcttggagt atgtttgcca caacccaagc cgatagagcg
     2521 gtaaggtctg caactgcacc taaagaaatg tggttccata agaagataat agatgaaaaa
     2581 acaggtaaag tatcctttga taccagacaa atttggtcat tgaatgattt aagcaaggaa
     2641 gaactggcaa gcattcaaga cacaaatggc aaagttatta ctgtgtctaa tcctggtatt
     2701 ttcaataatc gagaagattc attaagcaat gcagcaaaac aaaatcgtaa tagtacaaac
     2761 ggtagtggtg ttattgcagt tatgaatcct ccaacaggga aatataaatc tgattctaat
     2821 aacaaaataa aagattcttt atggctcagt tcaagtcttg tttctgaact gatgtatgtc
     2881 ggttacgacc aattaaataa taaagtgttc caaggctatt tacccaaaac caattcagaa
     2941 aaactgaatc aagatattta tcgagaggtt caaaaaatgg ataatggctg gtctgttgat
     3001 accagtaacc acagtcgtgg gggaattaca gcaagcgttt ccctaaaaga ttgggtaaac
     3061 aataaagagc aaaatggcat tgccccaatc agaaaagcac gtttctatgg tacagccaca
     3121 aatgtgaaga atgattacgc cgatgtttta cagaaaaacg gctataccta tacgggtgca
     3181 gacggtaaaa cttataacag cggagcctac tcaatcgtgc atgataaaga ttttgtgggg
     3241 aacaaat
//
LOCUS       DAXHMX010000183         3230 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHMX010000183 DAXHMX010000000
VERSION     DAXHMX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.183"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(245..1096)
                     /locus_tag="ACFM4S_001980"
     CDS             complement(245..1096)
                     /locus_tag="ACFM4S_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6051592.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1444..2958
                     /gene="katA"
                     /locus_tag="ACFM4S_001981"
     CDS             1444..2958
                     /gene="katA"
                     /locus_tag="ACFM4S_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6051593.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            3034..>3230
                     /locus_tag="ACFM4S_001982"
     CDS             3034..>3230
                     /locus_tag="ACFM4S_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051594.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRE"
BASE COUNT          779 a          913 c          830 g          708 t
ORIGIN      
        1 gccattctca caaaaacaga aattcaaaaa cagaaatccc aaacccccgt cattcccgcg
       61 aaagcgggaa tccaggttcg ttgagtttcg gtcatttccg ataaattctt gcagctttgc
      121 gtttctagat tccctctttc gcgggaatga cgggatgcag gtttccgcgc ggacggattc
      181 ggcattcccg ctttcgcaga aatgccggct tttggtttgt tgtctttata agattattcg
      241 gcaattattc tgcggttttg cgtttgtgtg ctgccggaat ctcaaaggat tctctgtatt
      301 tggcaatggt gcggcgagaa acctccatgc cgcggaaagc cagcaggttg gcgagcgcct
      361 cgtcagaata cggcgtgggc gtggacgagg caaaaccggc ggcgggaaag ggattgagcg
      421 aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc ggtttggggc aggcgttttt
      481 ttattcgcgc gagggtttgg ctgcggttgc cgtcaatgct gtcgagggcg tttcggacga
      541 tatgcagggc ggagggtttg gcagcacatt cgcccgatct ttctatctgc agtatcagcg
      601 attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa ttttttcaat gcggtcaggg
      661 cttgtttcag cattgcttca tccaacatcc actccaaggg cgtatggtcg aggatgtctt
      721 cgatgctgtc ggtcagataa ccttgctcgt caaggaaatc gatgaggatg tggacacagg
      781 cggattcttg gtcggaaagc gggtgttcgc atacttgcgc gtgcaggtat tgcttgaaat
      841 cctgctcgcc ggcgatgttg gacagcatat cttcgccttc gtctccgccg atttgacggg
      901 caggcgcagt gtaatggctg aactcggcat cggaaaattc atccgtgtct ttgcgttcga
      961 gcagggggtt gtccgacagc cagttttcga cctcgcgttc aagttcgata cccgacatct
     1021 gcaatacgcg caaagattgt tgcagccgct ggttgagctg ctgggtctgt ttgagcttta
     1081 ttccgagtaa ggtcatgata atgtggggaa aattgttatt ttcagcctgt cggcgcaaaa
     1141 aatgccgcaa agcgtcattg cattataaat ggttttaatg agcgggttcg gattccgttc
     1201 gataacaaaa aacaaacgaa aatcaagaac cgattgctta taataatatt aaatcgattt
     1261 catagtttta atagcgaaaa tcttggcgta tagtcgcatc catagttttt acaaaaggga
     1321 aataaaatgt cgattcaaga aatttattgc aatcaagaaa ccggttacga atacgctttc
     1381 cgccaaatcg tactgtaagc cgtttacccc catttgacca acctgagaaa aggaacaaga
     1441 gcgatgacta cctccaaatg ccctgtaacc catctgacca tgaacaacgg cgcgcctgtt
     1501 gccgacaatc aaaacagcct gaccgccggt cctcgcggcc ctctgctggc gcaggatttg
     1561 tggctgaatg aaaaactcgc cgacttcgtg cgcgaagtca tccccgaacg ccgtatgcac
     1621 gccaaaggtt cgggcgcgtt cggtacgttt accgtaacgc acgacatcac caaatacacc
     1681 cgcgccaaaa tcttcagcga agtcggcaaa aaaaccgaga tgttcgcccg tttcaccacc
     1741 gtggcaggcg aacgcggcgc agccgatgca gaacgcgaca tccgcggttt tgccttgaaa
     1801 ttttataccg aagaaggcaa ctgggatgtg gtcggcaaca acacgcccgt gttcttcctg
     1861 cgcgacccgc gtaagttccc cgacctgaac aaagccgtca aacgcgaccc gcgcaccaat
     1921 atgcgctctg ccacaaacaa ctgggacttc tggacgctgc tgcccgaagc actgcaccaa
     1981 gttaccatcg tgatgagcga ccgcggcatc cccgccggct accgccatat gcacggcttc
     2041 ggttcgcata cctacagctt ctggaacgaa gcaggcgagc gtttttgggt gaaattccat
     2101 ttccgcaccc aacaaggcat taaaaacctg accaacgaag aagccgccaa aatcatcgcc
     2161 gacgaccgcg aaagccatca gcgcgactta tacgaagcca tcgaacgcgg cgagtttccg
     2221 aaatggacga tgtacatcca agtcatgcct gaagcagacg cggaaaaagt accttatcat
     2281 ccgtttgact tgaccaaagt ttggccgaaa aaagactatc cgctgattga agtgggcgaa
     2341 ttcgagttga accgcaatcc cgaaaacttc ttcgccgatg tggaacaatc cgccttcgca
     2401 ccgagcaacc tcgttcccgg tgtcggcgcc agcccagata aaatgttgca agcccgtttg
     2461 ttcaattacg ccgacgcgca acgctaccgc ttgggcgtga acttccgcca aatccctgtc
     2521 aaccgtccgc gttgccctgt tcacagcaac cagcgcgacg gacaaggacg cgccgacggc
     2581 aactacggca gcctgccgca ctacgaaccc aacagcttcg gccaatggca gcagcagccc
     2641 gacttcgccg aaccgccttt gaaaatcaac ggcgacgcgg cgcactggga ctaccgccaa
     2701 gacgatgacg actatttcag ccaaccgcgc gccctgttca acctgatgaa cgacgcgcag
     2761 aaacaggcat tgttcggcaa caccgccgcc gcaatgggcg acgcgcccga cttcatcaaa
     2821 taccgccata tccgcaactg ctaccgttgc gatccggcat acggcgaagg cgtggcaaaa
     2881 gccctcggac tgactgtcga agacgcacaa gccgcccgcg cgaccgaccc cgcactgggt
     2941 caggccggtt tgctgtaagg gggcattatg tggatggaaa ttgaagaaat cctgtcccgc
     3001 gccgtccgct accggaaata accgggcata aaaatgccgt ctgaaacgtc gtttgaccgc
     3061 ttcagacggc atttccccat cccgcccccg ccgtttcagt gggcgttttt tattaaacgc
     3121 aaaatatccc gtcattcccg ccccacagcc cacttttccg ccgtcattcc cgcaccatcc
     3181 ccaccggttc aaaccggcac gaaaactttt ccgcgtcatt cccgcgaaag
//
LOCUS       DAXHMX010000184         3190 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHMX010000184 DAXHMX010000000
VERSION     DAXHMX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.184"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            67..1020
                     /locus_tag="ACFM4S_001983"
     CDS             67..1020
                     /locus_tag="ACFM4S_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6051595.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1131..1454)
                     /locus_tag="ACFM4S_001984"
     CDS             complement(1131..1454)
                     /locus_tag="ACFM4S_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051596.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1627..2988)
                     /locus_tag="ACFM4S_001985"
     CDS             complement(1627..2988)
                     /locus_tag="ACFM4S_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6051597.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
BASE COUNT          750 a          933 c          769 g          738 t
ORIGIN      
        1 gtactggttt aaatctaatc cactatagat taaaatcaca caatatccaa ctattcggac
       61 cttcatatga cacataccgt ccacctgcat ttagaagaaa ccgacaactt ggcgttgcag
      121 cgtctgtgcg gttcttttga caacaacctt gatttacttg ctaaagcact cgatatccac
      181 atcagccgcc gttttgaaca tttcactttc aacggcgcat ttgcacacgc cggcaaacgc
      241 gcactgctca aactcttgga aacggcgcag acgcgcgacc taaacgacgg cgacatcagg
      301 cttgccgccg tcgaagccca aaccgaagat gccggtcatc aagaaaaaaa ccatgaccac
      361 gcctattatt tccgcaccaa gcgcggcagc atcggcggca gaacgccacg gcagaacggc
      421 tatatccgcg ccctactcaa ccacgacatc gtattcggtc tcgggccggc aggtacgggc
      481 aaaacctatc tcgccgtcgc cgccgccgtc gatgcgatgg aaaaacacca agtcgaacgc
      541 atcattttag tgcggcctgc cgtcgaagcc ggcgagaaac tgggttttct gcccggtgac
      601 ctgacccaga aagtcgatcc ctaccttcgt ccgctttatg atgccctcta tgacctgatg
      661 ggctttgacc gtgtaaccaa gctgattgaa aaaggcctga ttgaaatcgc cccgctcgcc
      721 tatatgcgcg gcaggacgct caacggcgca tacatcatcc tcgacgaagc gcaaaacacc
      781 acgcccgaac aaatgaaaat gttcctgacc cgcatcggct tcggcgcgaa agccgtcatt
      841 accggcgaca ccagccaaat cgacctgccc aaaaacatca aatcgggatt aaaagatgcg
      901 cgtgagaaac tgcacaacgt ggaagggctg tatttccaca cctttaccgg cgaagacgtt
      961 gtccggcatc ctctggtgca aaaaatcgtc gaagcctacg aatcggcaga acacgactga
     1021 cattccaatg ccgtctgaaa cccgatgcat ggaaaatcgg gcagagaaaa acagatacgg
     1081 aagccaagct ccgtatccgt atccgtttct ttatccgtcc aaccgataca tcaatcgaat
     1141 tttctgcatc ggattgcctc atcttcgcaa aacattgccg aagtttcgcg gttgcacgcc
     1201 tgacgcactt tctgccgcgc cacgccttca ttccgatcgc ttgcctcaaa cgtcttctga
     1261 aaaggcgtaa cgctgcatac atacatggtg gaatttgaac cgaaaaggtc gattttgacg
     1321 gtattgctgc cgctttccaa tacggcgaca cggtgttcca actgcgccag acgtgcttcc
     1381 aaatcgccga ttctgtcggc aaatgcaggc atggcggcac acgcggtcaa aatgcccaaa
     1441 atacattttt tcatcaattt ttccttgttt cttgaaccct gcccacccgg acatcctttt
     1501 tccgagaggc agggttagac tttatttacg gggatgaatc cccttacaaa tcaggataat
     1561 acatagaacg gcactttatg cttcatccca tgtgttgaaa cttcattgct ccttgtctgt
     1621 tgacccttat tcccaatccg ttcccacaac cttcggcttg ggtttgcgcc tgcgacggcg
     1681 gcgttttttc gcctgtcctt cgtttttttc gtgtcgggcg gcggcttcgt ttttggtcat
     1741 ctccgaccgc tgttccgtcg atgccgtctg aaacgccgtc caccactcgg caagggcgcg
     1801 gtccgcattg ccggtttcgg cgcgcaagag caggaaatca taggcggcac ggaaacgcgc
     1861 ctgtgcaaac agtttgtgcg gacgtgcgcc tttgcgattt tcaaactgcg gctggaacat
     1921 ccaaatttcg cgcatcgtgg cggaaaagcg ttgcggcacg ccccaaccgc gttcgacggt
     1981 ttcgcgcatc gtattgattg catcggacag ggcgggcacg ggtttcaaac cctgttgcag
     2041 attgcttttc caatggcgtt ccaactcggg ccacatcagg gccgccagta cgaaaccgac
     2101 cgaaaccgat ttgtcggcac gcagccgctc atcggtattt ttcagggcaa gcaccgtcat
     2161 ttttccggcg atgccgtctg aaacgcgcaa ggcattgagc agcggatgga tgtcgtccgg
     2221 tatgtcaaat ccgttcaaac gtttcagaca ctcgcgcgcg tgccctgaaa acagcagttt
     2281 cataatttcg tcgaacagcc gcgctaccgg ttcgtgcttc agacggcata tcgattcggc
     2341 aatcggtgcg gcggtttctt ccgacagatc aaagcccaat ttgctcgaca ggcggatggc
     2401 gcgcaaaatc ctgacagggt cttcctgata gcgttcggcg gcatcaccaa tcataaccag
     2461 cctgcgggcg gcaacatcgg caatcccgtt gtggaaatcc aaaatctctt ctttttcagg
     2521 atcgtaatac aaggcattgc aggtaaaatc gcgccgcatc gcatcttctt cgatgctgcc
     2581 gtaggtgttg tccttcatga ttctgccgtg cgcgttctga tgaaccttcg caccgccgcg
     2641 aaacgtcgtt acttcgataa tctccgcacc gttcatcaca tggacaatct gaaaacgcct
     2701 gccgatgatg cggctgcggc ggaagagttt gtgcacctgt tcgggcgtgg catcggttgc
     2761 gacatcgaaa tccttgggtt cgatgccgag cagcaggtcc ctgaccgcgc cgccgaccac
     2821 ataagcctga aaccccgccc ctttcaggcg gcgtatgacg ttttcggcgg caaagctcaa
     2881 catttcggca cggatgttgt gtctttcggc aggaatgatt gttttacttt ccgctttttt
     2941 actgctccga ccggaaggca gcatcttatt caaccatttt ttcagcataa atattctaac
     3001 ccgaagagcg gcatctccac gggcaaccgt gttcagacgg catcaggcgg tacgattccg
     3061 caagatgccg taaccataaa caaaaggcgt agaaactacg ccgtccgaat cacgccgccc
     3121 tcgcggcggc ggcaacgcgc cattataaca gattgccctt taaaaaaatc agaccctgct
     3181 tttgtggcgg
//
LOCUS       DAXHMX010000185         3107 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHMX010000185 DAXHMX010000000
VERSION     DAXHMX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.185"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..212)
                     /locus_tag="ACFM4S_001986"
     CDS             complement(<1..212)
                     /locus_tag="ACFM4S_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051598.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQA"
     gene            272..895
                     /locus_tag="ACFM4S_001987"
     CDS             272..895
                     /locus_tag="ACFM4S_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6051599.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1006..2388
                     /locus_tag="ACFM4S_001988"
     CDS             1006..2388
                     /locus_tag="ACFM4S_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6051600.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSIITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2392..2916
                     /locus_tag="ACFM4S_001989"
     CDS             2392..2916
                     /locus_tag="ACFM4S_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6051601.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          618 a          839 c          945 g          705 t
ORIGIN      
        1 gcctgctgcc gcccgaaaag cgtgatgcgc tgatggtgct gctgcataat ccggacgacg
       61 gataaccggt gaaaacggaa atgcccgggc ggtgttgcct gtgcctgttt ggacaagcgt
      121 tcttgagggc ggtagaattg aggtttgccc gaaaagggca gggcgatatg ccgcgcttgg
      181 attttgcgcg cggtttttcg cgttggaagc aatcattgtc tgtttgaata tttaatgccg
      241 tctgaaacgt ccggacgcgc ccccgttccc tatgaaaaaa ccgaccgata ccctacccgt
      301 taatctgcaa cgccgccgcc tgttgtgtgc cgccggtgcg ttgttgctca gtcctctggc
      361 gcacgccggc gcgcaacgtg aggaaacgct tgccgacgat gtggcttccg tgatgaggag
      421 ttctgtcggc agcgtcaatc cgccgaggct ggtgtttgac aatccgaaag agggcgaacg
      481 ttggttgtct gccatgtcgg cacgtttggc aaggttcgtc cccgaggagg aggagcggcg
      541 caggctgctg gtcaatatcc agtacgaaag cagccgggcc ggtttggata cgcagattgt
      601 gttggggctg attgaggtgg aaagcgcgtt ccgccagtat gcaatcagcg gtgtcggcgc
      661 gcgcggcctg atgcaggtta tgccgttttg gaaaaactac atcggcaaac cggcgcacaa
      721 cctgttcgac atccgcacca acctgcgtta cggctgtacc atcctgcgcc attaccggaa
      781 tcttgaaaaa ggcaacatcg tccgcgcgct tgcccgcttt aacggcagct tgggcagcaa
      841 taaatatccg aacgccgttt tgggcgcgtg gcgcaaccgc tggcagtggc gttgattttg
      901 aacccgcgcc gcaaccgaaa tacggcgaat cctgtataat ccgaaaatct gttcactgga
      961 agttcagacg gcattgcaac tgttgatgcc gtctgaaaaa atatgatggc aagagacaac
     1021 cgcatccaaa tgtttccgca cgaatggcgc gccagtacga cgctttccgg cgtgtacgcg
     1081 ctgcgtatgc tgggtatgtt cctcgtgctg cccgtattgg cggtgtatgc cgcctcgctg
     1141 cccggcgcgg aaggcaacaa aacgctggtc gggctggcaa tgggcattta cgggctgaca
     1201 caggctctgc tgcaactgcc tttgggcatc gcttccgaca agttcgggcg caagaaaacc
     1261 atttatgtcg ggctgattgt gtttgcagcg ggcagcttcc ttgccgccgc cgccgatacg
     1321 ctgcccatgc tggtcgccgc acgcgccata cagggtgcgg gagcggtcag tgcggcggtt
     1381 accgcgctgc tggcggattt gacgcgcgac ggagtacgta cccgcgcgat ggcgatgatc
     1441 ggtttgagta tcggtctgac gttttcggtc agcctcgtcg ttgcccctgt gattgccgac
     1501 gcggtgggcg tacgcggctt gtttctgctg accggtattt tgaccgttgc cagtatcggc
     1561 gtggtggcgt ggatgactcc cgacccggag gtttccaagc tgcacgaaga tacgcaggcg
     1621 cagccttcgc gcataggcga agttttgaaa aaccgtaggc tgctgacgct cgatttcggc
     1681 attttcgccc tgcacgccgc gcaaatggca ttgtttaccg cgctgccttt tgcgatgacg
     1741 cagctcggtt tggaaaaaat ccagcattgg aaagtctatc tgccctcgat cattacgggc
     1801 ttggtggtga tggttccgct gattatcgtc ggcgagacgc gcaacaagct caaacaggtt
     1861 tttgttttgg gtatcgtctg tattgcggcg gcgcagctcg gtttgctgtc cggtatgcgc
     1921 tcggtaggct tgattaccgc ttatttggtt gtttacttta tcggttttaa tgtgttggaa
     1981 gcgagcctgc cgtctatggt ttccaaaatc gcgccgtccg acctgaaggg tacggcgatg
     2041 ggtgtgtaca acacgatgca gtcgctcgga ctgtttgccg gcggcgcggc aggcggcttg
     2101 ctgtttcaaa aatacggctt ttccggcgtg tttgcctttt gcagcatatt gatgctgctg
     2161 tggctggtaa ttgccgtttt atcgcctgcg cccaagcccg tcaaaaacct cagttaccct
     2221 gtcggcggcg tgtggcaggg caatcgggac ggtttgcaac gcgccttgtc ggagcttgag
     2281 ggcgtggaag acatcggttt cagtttcgac gggcagaccg tctatcttaa agtgttgcag
     2341 aagggtttcg atcaggctgc cgctgaaaaa atcatcacag gagtttaaaa aatgtcattg
     2401 aacaaagtca tcctcatcgg ccgcctcgga cgcgatcccg aagtgcgcta tatgcccaac
     2461 ggcgaggcgg tgtgtaattt cagcgtcgcc accagcgaaa cttggaacga ccgcaacggc
     2521 caacgtgtag agcgtactga gtggcacaac atcacgatgt accgcaaact ggcggaaatt
     2581 gccgggcaat acctcaaaaa aggcgggctg gtttatctgg aaggcagaat ccaaagccgc
     2641 aaataccaag gcaaagacgg catcgaacgc accgcttacg atattgtcgc caacgaaatg
     2701 aaaatgttgg gcgggcgcaa tgaaaacagc ggcggtgcgc cttacgagga aggttacggt
     2761 cagagtcagg aggcttacca acgccccgcg cagcaaagcc ggcagcccgc ctccgacgcg
     2821 ccgtcccatc cccaagaagc accagccgcg ccgcgccgcc aacccgtgcc tgccgccgcc
     2881 ccggtcgagg acattgacga cgatatcccg ttctgaattt tacggccgga catcctcgcg
     2941 gagggaaatc ataaaggacg gagaaacctt aaaccttacg gggcagggtt tctctttttt
     3001 gcatttgggc cggcttgttg ccgccctttg cgttgggcgg aacgttgcga ttgcgccgtt
     3061 cgggcgtttt gaggcagtga tgcctgccgc acgtccccgc tgttttc
//
LOCUS       DAXHMX010000186         3096 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHMX010000186 DAXHMX010000000
VERSION     DAXHMX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.186"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..236
                     /locus_tag="ACFM4S_001990"
     CDS             <1..236
                     /locus_tag="ACFM4S_001990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051602.1"
                     /translation="HDDRGFSDTEVFYRLIVEILPIFFPIPSGLQMVDDSVCFSAVCR
                     SDQGAAVEGAGVGFLKNLVPNILFQIRGRSPEN"
     gene            complement(274..651)
                     /locus_tag="ACFM4S_001991"
     CDS             complement(274..651)
                     /locus_tag="ACFM4S_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6051603.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRRKYPYPMDIPRDIVIGIGSIIEFLMVPNWKLFTIKSSPWLPKSIKINERYERFR
                     VMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(682..1080)
                     /locus_tag="ACFM4S_001992"
     CDS             complement(682..1080)
                     /locus_tag="ACFM4S_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6051604.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGSIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            complement(1086..1732)
                     /locus_tag="ACFM4S_001993"
                     /pseudo
     CDS             complement(1086..1732)
                     /locus_tag="ACFM4S_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
     gene            complement(1896..2213)
                     /locus_tag="ACFM4S_001994"
     CDS             complement(1896..2213)
                     /locus_tag="ACFM4S_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051605.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(2216..>3096)
                     /gene="mafB"
                     /locus_tag="ACFM4S_001995"
     CDS             complement(2216..>3096)
                     /gene="mafB"
                     /locus_tag="ACFM4S_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6051606.1"
                     /translation="YRLNWKGHEHHPADGYDGPQGGNYPKPTGARDEYTYHVNGTARS
                     IKLNPTDTRSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGVA
                     AGALNPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
BASE COUNT          779 a          685 c          605 g         1027 t
ORIGIN      
        1 aacacgacga tcggggtttc agcgacaccg aggtatttta ccgtctaatc gtggagattt
       61 taccgatttt ctttcctatt ccatccggtc ttcaaatggt tgatgacagt gtttgctttt
      121 cagccgtctg ccgttctgac caaggtgctg ccgtggaagg agcaggtgta ggatttttga
      181 agaacttagt tcccaatatc ctgtttcaaa taaggggaag gtcgcctgaa aattaaacac
      241 ggtttcagac ggcattttga ttgccggggt ttgctatttt ttgttgtaat aatcaaatcg
      301 tacgttgact atgtctttct cggtaaaaat ataacggagc attactctga acctttcata
      361 acgctcatta attttgatac ttttaggcaa ccaaggggaa gatttaattg taaaaagttt
      421 ccaatttgga accattaaaa actcaataat gctaccgatt ccaatcacga tatcccttgg
      481 tatatccatc ggataaggat attttctcct aacctcgatt aaagcattct ccaatttcca
      541 atattcttca tcatcccaca ccccgtcatc ataccatttg ccaataaatg aattttcgtc
      601 atactcctta aaacaaggga tgtttcttct aaaatccttg aactcgcaca taataatgta
      661 tctccaatat aattaaactt ttcatctcaa tctaccttta ctatgttgta ttggaaagta
      721 aaaaaatttc cagtcctcta catctagatc agtaaaaata taacggagca ttactctgaa
      781 cctttcataa cgctcattaa ttttgacact tttaggcaac caaggggaag ctttaatttc
      841 aaaaagtttc caattttgaa ccattaaaaa atcaataatg ctacctattc caatcacgat
      901 gtcccttggt atatccatcg gataaggata ttttctccta acctcgatta aatcattctc
      961 caatttccaa tattcttcat catcccacac cccgtcatca taccatttgc caataaatga
     1021 attttcgtca tactccttaa aacaagggat gtttcttcta aaatccttga actcgcacat
     1081 cataattaat ctccaatacg atttaggttt ttatcaaatg taccgtttct tgtttctttt
     1141 ctgttcagct tcttgggtga agatgcttct ttccaagccc ctccattatg tgaatctaca
     1201 tcgcgtgata tataactctt tcctttttta aaaatagcag catcatttct cgttctttct
     1261 tttatttttc tatatcccaa tttctttgct gctgcatatg cttctgaatc attcccatat
     1321 acgggggtag atggtgtttt tcttggcgga cacgcattat gaacccaaac cccttccgtt
     1381 ccgcctgatt acccttgacg aagtaggtat gccagtcggc aacggtcaga ttgtaggctt
     1441 tgagcggctg ctgtttgagg gtaatgtttt gaaccgtctg ttttgcaccg ctttcggaaa
     1501 gcagggtgtc gccttttttc agacgacctg cctgtatcca ctttccttga ctgtaaaacg
     1561 ggtggatttt attggaaacg agggtctgta ttttaccgat gccgtctgaa atttcaatgt
     1621 aaacggtttc ttgatacgga ttgccgtatc gggcggtaac gggtttgtat cccgtttttc
     1681 cgcttgcctc gtccttggcg aagacgcggt cgccggttcg gatacgggca atggcttttc
     1741 agccgtctgc cgttttgacc aaggtgctgc cgtggaagga gcaagtgtag gatttttgaa
     1801 gaactcagtt ctcaatatcc tatttcaaac gaaggaaatg ccgtctgaaa attaaacacg
     1861 gtttcagaag acattgtgat tgttggtttt ttaacctacg cttgcgaaat taattccttt
     1921 ctcctgaata ctgcaccatt tatttggttt aactccatag aagaatgatt agaataaata
     1981 cattcaaagt cagcttcatc aaaatattct ccacacatag accataactc tgtaatcaaa
     2041 tattccatat cttcatggtc ttctgtgatg gcttctcttg ataaataatt tttgattttt
     2101 aaaaaccttc ttccttcctc gtaatctacg gtaattaacc tgatatctga gtgtatcatg
     2161 cccagcattg ctatattcat accgactcgc agccagcttg gtaatatatt cataactatt
     2221 gcaccttttt aatggtttta gggttagccg gaacaatgtg aactccattt ttacctgaat
     2281 gaatcgtacc aaaattggta cttaatcctg atttcccatc gctgcctata ggatacccaa
     2341 aatcaacaac aggatttcct cttgctcctt tagaaataac tggataagca cctgaatgta
     2401 ttccgttcaa caattcttga ggattaatgt tttgatttaa agtactctta ccttcaatgt
     2461 agtttctatg tcctgaaata tgtttccctt gagctccatc atgaatttta gtcccaatag
     2521 attttgcttt aaaatcaaca ttgcgggttt gaaacatttc tccatctaca gactgagata
     2581 atcttgaagc agtgttatgc ttgtaggagt ctgagaaatc cccactaacc gcagccttcc
     2641 ccggttttgc cgcctttgtc aggtttttga ctttggcaaa cggcaggacg ttgaccaggg
     2701 cttcgacggt ttcggcggca ttggggtttt cctgcatcca ctggtcaacg gcttcgcccg
     2761 cgctgataaa ggggttgagc gcgccggcgg cgacgccgtt gataaactcc atgctgttgc
     2821 cccagcggtc gagcttggca ttgtgctcga acatttttct gttggcttca tcggcgcggt
     2881 cggagaaatt gctgccgagg ttgctgtaat tgtcggatat gcgttgccgg atgctgcggg
     2941 tgtcggtcgg attgagtttg atactgcggg ctgtgccgtt gacgtgatag gtgtattcgt
     3001 ctcgtgcgcc cgtaggtttg gggtaattgc cgccctgcgg gccgtcatag ccgtcggcgg
     3061 gatgatgttc gtgccctttc cagttgaggc ggtata
//
LOCUS       DAXHMX010000187         2978 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHMX010000187 DAXHMX010000000
VERSION     DAXHMX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.187"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            113..2878
                     /gene="tdfH"
                     /locus_tag="ACFM4S_001996"
     CDS             113..2878
                     /gene="tdfH"
                     /locus_tag="ACFM4S_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6051607.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQDGQLKFNSDSGKWER
                     DLQRQQWKYPWYKKYENPQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYREHCNQSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
BASE COUNT          846 a          750 c          726 g          656 t
ORIGIN      
        1 ccaattgaaa tcttcagacg gtatatcgaa tttacacttt ttaatgttta tgccgcctaa
       61 aaaaatgcta atatatttct taattgtctg actgtttatt gttgaggaaa atatgagatc
      121 ttctttccgg ttgaagccga tttgttttta tcttatgggt gttatgctat atcatcatag
      181 ttatgccgaa gatgcagggc gcgcgggcag cgaggcgcag atacaggttt tggaagatgt
      241 gcacgtcaag gcgaagcgcg taccgaaaga caaaaaagtg tttaccgatg cgcgtgccgt
      301 atcgacccgt caggatatat tcaaatccag cgaaaacctc gacaacatcg tacgcagcat
      361 acccggtgcg tttacacagc aagataaaag ctcgggcatt gtgtctttga atattcgcgg
      421 cgacagcggg ttcgggcggg tcaatacgat ggtggacggc atcacgcaga ccttttattc
      481 gacttctacc gatgcgggca gggcaggcgg ttcatctcaa ttcggtgcat ctgtcgacag
      541 caattttatt gccggactgg atgtcgtcaa aggcagcttc agcggctcgg caggcatcaa
      601 cagccttgcc ggttcggcga atctgcggac tttaggcgtg gatgacgtcg ttcagggcaa
      661 taatacctac ggcctgctgc taaaaggtct gaccggcacc aattcaacca aaggtaatgc
      721 gatggcggcg ataggtgcgc gcaaatggct ggaaagcgga gcatctgtcg gtgtgcttta
      781 cgggcacagc aggcgcagcg tggcgcaaaa ttaccgcgtg ggcggcggcg ggcagcacat
      841 cggaaatttt ggcgcggaat atctggaacg gcgcaagcaa cgatattttg tacaagacgg
      901 ccagttgaaa ttcaattccg acagcggaaa atgggagcgg gacttgcaaa gacaacagtg
      961 gaaatacccg tggtataaaa aatacgaaaa cccccaagaa ctgcaaaaat acatcgaaga
     1021 gcatgacaaa agctggcggg aaaacctggc gccgcaatac gacatcaccc ccatcgatcc
     1081 gtccagcctg aagcagcagt cggcaggtaa tctgtttaaa ttggaatacg acggcgtatt
     1141 caataaatac acggcgcaat ttcgcgattt aaacaccaaa atcggcagcc gcaaaatcat
     1201 caaccgcaat tatcaattca attacggttt atctttaaac tcatatgcca acctcaatct
     1261 gaccgcagcc tacaattcgg gcaggcagaa atatccgaaa gggtcgaagt ttacaggctg
     1321 ggggctttta aaagattttg aaacctacaa caacgcgaaa atcctcgacc tcaacaacac
     1381 cgccaccttc cggctgcccc gcgaaaccga gttgcaaacc actttgggct tcaattattt
     1441 ccacaacgaa tacggcaaaa accgctttcc tgaagaattg gggctgtttt tcgacggtcc
     1501 ggatcaggac aacgggcttt attcctattt ggggcggttt aagggcgata aagggctgct
     1561 gccccaaaaa tcaaccatcg tccaaccggc cggcagccaa tatttcaaca cgttctactt
     1621 cgatgccgcg ctcaaaaaag acatttaccg cttaaactac agcaccaata ccgtcggcta
     1681 ccgtttcggc ggcgaatata cgggctatta cggctcggat gacgaattta agcgggcatt
     1741 cggagaaaac tcgccgacat acagggaaca ttgcaaccag agctgcggaa tttatgaacc
     1801 cgtattgaaa aaatacggca aaaagcgcgc caacaaccat tcggtcagca ttagtgcgga
     1861 cttcggcgat tatttcatgc cgttcgccag ctattcgcgc acacaccgta tgcccaacat
     1921 ccaagaaatg tatttttccc aaatcggcga ctccggcgtt cacaccgcct taaaaccaga
     1981 gcgcgcaaac acttggcaat ttggcttcaa tacctataaa aaaggattgt taaaacaaga
     2041 tgatacatta ggattaaaac tggtcggcta ccgcagccgc atcgacaact acatccacaa
     2101 cgtttacggg aaatggtggg atttgaacgg gaatattccg agctgggtca gcagcaccgg
     2161 gcttgcctac accatccaac accgcaattt caaagacaaa gtacacaaac acggttttga
     2221 gttggagctg aattacgatt atgggcgttt tttcaccaac ctttcttacg cctatcaaaa
     2281 aagcacgcaa ccgaccaact tcagcgatgc gagcgaatcg cccaacaatg cgtccaaaga
     2341 agaccaactc aaacaaggtt atgggttgag cagggtttcc gccctgccgc gagattacgg
     2401 acgtttggaa gtcggtacgc gctggttggg caacaaactg actttgggcg gcgcgatgcg
     2461 ctatttcggc aagagcatcc gcgcgacggc tgaagaacgc tatatcgacg gcaccaacgg
     2521 gggaaatacc agcaatgtcc ggcaactggg caagcgttcc atcaaacaaa ccgaaaccct
     2581 tgcccgccag cctttgattt ttgattttta cgccgcttac gagccgaaga aaaaccttat
     2641 tttccgcgcc gaagtcaaaa atctgttcga caggcgttat atcgatccgc tcgatgcggg
     2701 caatgatgcg gcaacgcagc gttattacag ctcgttcgac ccgaaagaca aggacgaaga
     2761 cgtaacgtgt aatgctgatg gcttgtgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgataacg atgagctaca agttttaaag
     2881 gcagcccgcg ttttgtggaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaa
//
LOCUS       DAXHMX010000188         2974 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHMX010000188 DAXHMX010000000
VERSION     DAXHMX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.188"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            180..402
                     /locus_tag="ACFM4S_001997"
                     /pseudo
     CDS             180..402
                     /locus_tag="ACFM4S_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(463..1416)
                     /locus_tag="ACFM4S_001998"
     CDS             complement(463..1416)
                     /locus_tag="ACFM4S_001998"
                     /inference="COORDINATES: protein motif:HMM:NF042780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC6051608.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            1799..2818
                     /gene="galE"
                     /locus_tag="ACFM4S_001999"
     CDS             1799..2818
                     /gene="galE"
                     /locus_tag="ACFM4S_001999"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6051609.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFKIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLSV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRA
                     FEAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLSQMMEDSWRWVSNHP
                     NGYDD"
BASE COUNT          876 a          579 c          585 g          934 t
ORIGIN      
        1 gccccggatg cctgattatc gggtatccgg ggaggattaa gggggtattt gggtaaaatt
       61 aggaggtatt tggggcgaaa acagctgaaa acctgtgttt gggtttcggc tgtcgggagg
      121 gaaaggaatt ttgcaaaggt ctcaggctgc atttctcaat gtaaaacgcg atattttcac
      181 ccattattag ggataagtag ccagtggttt acactggcta ctttcatagt tatgaagtac
      241 tgggtctgag tgaattcaat tatttccgta taaaacacag tagacatttt actaatcggc
      301 aaaattatat tagaagaatt gagaactact gaagtcaggt aagataccat tttgcacaaa
      361 tttaacggca tccctaaaaa gcattttggg tggtatttaa aggagtacaa aatggtttta
      421 actacagtat gcttagaaat ctatagcctt acgcgcttta tattaaatca cctttctcaa
      481 ccatttagaa tcataaaaaa actttttagg atccctaatt aactttttaa attttcttaa
      541 aaaaatagtt ttagttgatt ctatactatt caccgcagat aaatattctt ggtaatcatg
      601 ttgcaatggc acaacctgct ttaatagctt attaaactca tcagttgtaa tcaccttttt
      661 agtagcatag caatcaagcc aatccggatc tgctattttt ggggaagtga aatctatttg
      721 atcaaaataa actaaaaccc cactacctaa aaaatgctga tttgcatagg atttagaaaa
      781 gcctaagtct gcaacaacac caacagggat acccttacat tcagcttcaa aaagcatagt
      841 agatgaataa gataaacaat accccatttc ttggaaggct tgcgcggtac tttttcgctc
      901 tattgtcaaa ttactcggca actgaaactg ctttgccagc tctatatacg aatgtttatc
      961 ctgatgcaca gtaatatctt tatctgcaac ccttagcaaa atagtaaatt ctttctcagg
     1021 gtatttttca gccaaggcaa tcaatttttt taatgtataa attctttctt cttttttaaa
     1081 tgggatttta acttggtcaa taaagtaaat tttctctcca tgacgacctt ttgatgcgtg
     1141 gcgaaaaatt ggataaccat aaagtatatt ttgacaactt aatttatatt gtttcttata
     1201 ttcctctgca attctaaaat catgcttatt attatataaa acaatatcag cccccatacg
     1261 agatagaata cttgcctgat caccgaatac tacacctgga aacaaggtaa taatcaatgg
     1321 ccttgaagca atatttaatt gcgcattctg cttaaagaac ctttttaaca acccatttcc
     1381 aaccgataaa attactgcgt cataatattg atgcatattc ttaataaagt aatcatcaat
     1441 atgaaaaaaa aaattgcttg gtctttatct atccctgatt ctaataactg tcgatttgaa
     1501 agaatgtttt ctcgactttt gtggatataa atatcaattt gagcatcttt tatctctttt
     1561 gcaacagcat agcccgagtt aaggaacgag tcataactgg caattagtaa tacttttttc
     1621 actattttcc cttaattcta caccatatga ataaaattat agtattttac cttagatatc
     1681 aaatatactt aaccaaaaaa atctgaagaa atctactgct acttcaggga taattatttc
     1741 attaagattt tacaaccatt attatcaatc tatcaaagta tttttagtat tttattctat
     1801 gaaaaaaatt ctcgttaccg gcggcaccgg ttttatcggc tcgcataccg ttgtttcttt
     1861 gctgaaaagc ggccatcaag tcgtgatttt ggataaccta tgcaattcca gcatcaatat
     1921 cctgccacgc ttgaaaacga ttaccggcca agagattccg ttttatcaag gcgatatccg
     1981 cgatcgtgag attttgcgcc gtatttttgc ggaaaaccgc attgattcgg tgattcattt
     2041 tgccggcttg aaagcggtgg gtgaaagtgt ggccgagccg atgaaatatt atgataataa
     2101 tgtttccggc agcttggtgt tggcggaaga aatggcgcgt gccggcgtat ttaagattgt
     2161 gttcagctca tcggctacgg tttatggcga tccgggcaaa gtgccttata ccgaggatat
     2221 gccactgggc gataccacca gcccttacgg cacatcgaaa tcaatggttg agcgcattct
     2281 cactgatatt caaaaagccg atccgcgctg gagcgtgatt ttgttgcgtt atttcaatcc
     2341 gattggcgcg cacgaaagtg gtttgattgg cgagcagcca aacggcatcc cgaataattt
     2401 gttgccttat atctgccaag tggcggcagg caaactgccg cagttgtcgg ttttcggcga
     2461 tgactaccct accccagacg gtacgggtat gcgcgactat atccatgtaa tggatttggc
     2521 ggaaggccat gtcgcggcaa tgaaggcaaa aggcggcgtt gccggcgtac atttgttcaa
     2581 cttgggttcg gggcgcgcct attccgtttt ggaaatcatc cgcgcatttg aagcggcttc
     2641 gggtttaacc attccatttg aaatcaaacc acgccgtgcg ggcgatttgg cgtgtttcta
     2701 tgctgaccct tcttatacga aggcacaaat cggttggcaa actcagcgtg atttaagcca
     2761 aatgatggaa gattcatggc gctgggtaag taatcatccg aatggctacg acgattaagt
     2821 tgacctgata caggccgtct gaaagagatg ttttcagacg gcctctttat ctgaaaaaca
     2881 cacattctgt ctgctataat ctgtttatat tttttggcta tcctctgaaa tttatgagaa
     2941 aaatccttgt taccggcggc gcgggcttta tcgg
//
LOCUS       DAXHMX010000189         2969 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHMX010000189 DAXHMX010000000
VERSION     DAXHMX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.189"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            123..1667
                     /gene="purF"
                     /locus_tag="ACFM4S_002000"
     CDS             123..1667
                     /gene="purF"
                     /locus_tag="ACFM4S_002000"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6051610.1"
                     /translation="MCGVLGLVSHEPVNQFLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1767..2258
                     /gene="greB"
                     /locus_tag="ACFM4S_002001"
     CDS             1767..2258
                     /gene="greB"
                     /locus_tag="ACFM4S_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6051611.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2317..2943
                     /locus_tag="ACFM4S_002002"
     CDS             2317..2943
                     /locus_tag="ACFM4S_002002"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6051612.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFHRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATTNRLSR"
BASE COUNT          714 a          907 c          772 g          576 t
ORIGIN      
        1 cttattctcc ataaacccac ctatctgtcc ttcgctcaaa tattgtcaac aattcaggca
       61 aaaaaccacc gtttacttta taattccgtt ttacccttaa acctcgaatc ggagaagacg
      121 atatgtgcgg cgtattaggt ttggtcagtc atgaacccgt gaaccagttt ctgtacgacg
      181 gcttgcagat gttgcagcac agggggcagg atgcggcggg cattgcaacg gcggaaggcg
      241 gtaccttcca tatgcacaaa ggcaaaggca tggtgcgcga agtgttccgc acacgcaata
      301 tgcgcgattt gaccggcaac gccggtatcg cccacgtccg ttatcctact gcgggcaacg
      361 caggcagcag cgcggaggcg cagcctttct acgtcagctc gccgttcggc atcgttttgg
      421 cgcacaacgg caacctcacc aacactgccg aactgtatga aaacgtgtgt aacaaacacc
      481 tgcgccacgt caacaccagc tccgattccg aagtcttgct caacgtattc gcgcacgaat
      541 tacgccgcga agtctctaaa aacgccgacc cgcaccggct caatgccgac aatattttca
      601 acgccgttgc ccaagtccac cgcctggtgc gcggcgcata cggtgtgatt gccatgattg
      661 cgggctacgg tatgctcgcc ttccgcgacc cttacggcat ccgcccgctg gtattgggtt
      721 cgcaaaccga cagcgaaggc agaaaatcct atgccgtcgc ctccgaatcc gtcgccttca
      781 acgcgcttac ctacgatttg gaacgcgaca tccggccggg cgaagcggta tttgtcggct
      841 ttgacggcac aatgattgcc cgccaatgca gcgaccgtgc caaactcagc ccctgccttt
      901 ttgaatatgt ttattttgcc cgccccgact ctgtgattga cggcgtatcc gtttaccaat
      961 cgcgcttgga tatgggcgtg tcgctggcgg aaaaaatcaa acgcgagctg cccgtggacg
     1021 gcatcgatgt cgtgatgccc attcccgaca ccagccgccc cagcgcgatg gagcttgccg
     1081 tccatctcga caaaccctac cgcgagggtt tgattaaaaa ccgctatatc ggccgcacct
     1141 ttattatgcc cggacaggcg acgcgcaaaa aatccgtgcg ccaaaaactc agcccgatgg
     1201 aaaccgaatt tgcaggcaaa agcgtgttgc tggtggacga ctccatcgtg cgcgggacga
     1261 ccagccgcga aatcgtcgaa atggtacgcg cggcgggcgc gcgcaaagtc tatatcgcct
     1321 ccgccgcgcc cgaagtgcgc tatcccaatg tgtacggcat tgatatgccc acgcgcgaag
     1381 agttgattgc caacgggcgc agcgcggcgg aaatcgccgc cgaaatcggc gcggacggca
     1441 tcgtatttca agacttgggc gatttggaag ccgtcgtcaa agcactcaat ccgaaaatcg
     1501 aatccttcga ttcgtcctgt ttcaacggca tttatcagac cggcgacatc gatgatgcct
     1561 acctcgaccg cctgtctgcc gagaaatccg gctgcggcgg tctgaaaatc cacccgagca
     1621 ggatggaaca cagcatcagc atcagcgatg cgggcgacga agaataaggc ttcccaaacc
     1681 gcgatgccgt ctgaaatccg aacgccgggt tttcagacgg catttcccat atcggcatca
     1741 catttcccgt ttcatccgca cacaatatga gtaccgaaac caaaaactac attacgcccg
     1801 ccggatggca ggcattgaaa gacgagcttt atcaactggt caacaaagaa cgccccgaaa
     1861 tcgtccaaat cgtcaactgg gcggcaggca acggcgaccg cagcgaaaac ggcgactatc
     1921 tttacggcaa acgccgtatg cgcgaaatcg accgccgcat ccgtttcctg accaagcgtt
     1981 tggagtccgc cgtcgtcgtc gatcccgaat tgcgcgaagc gaccgaccaa gtatttttcg
     2041 gcgcgaccgt cggcctgttg cgcggcgacg ggcgcgagca aaccgtcaaa atcgtcggca
     2101 tcgatgaaat cgataccgcg caaaacaaaa tttcttggat ttcgccgttg gcgcgttgcc
     2161 tgattaaagc gcgcgaaggc gacgaagtgg ttttgaacac gccggaaggg cgtgaggaaa
     2221 ttgaaatcct ttctgtcgaa tacatcaaaa tcgactgatc gggaatgccg tctgaaacgg
     2281 tgcaagcgcg ccgtttccca aataaaggaa taccgaatga ggaaaatccg caccaaaatc
     2341 tgcggcatca ccacaccgga agacgcatcg gccgccgtag cggcaggtgc agatgccgtc
     2401 gggctggtct ttttccaagg cagcagccgg gccgtcgata ttgcccgcgc caaaaaaatc
     2461 accgccgcac tgccgccgtt tgtcagcgtt gtcgcccttt tcgtcaacga aagcgcgcaa
     2521 aacatccgcc gcatccttgc cgaagtgccg atacacatca tccaattcca cggcgacgaa
     2581 gacgacacat tctgccgtca gttccaccgc ccctatatca aggccattcg tgttcagacg
     2641 acatcagaca tccgaaacgc cgccgaccgc ttccccgacg ctcaggcact gcttttcgat
     2701 gcctaccatc cttcggaata cggcggcacc ggacaccgct tcgactggac gctgctggcg
     2761 gaatattcgg gcaaaccgtg ggtacttgcc ggcgggctga cccctgaaaa cgtcggcgaa
     2821 gccgtccgca tcaccggagc ggaagcggtc gatgtatccg gcggcgtgga agcgtctaaa
     2881 ggcaaaaaag acccagccaa agtcgccgcc tttatcgcaa ccaccaaccg cctatcccgt
     2941 taaagcaaca aaaattgccg ccggaatga
//
LOCUS       DAXHMX010000190         2939 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHMX010000190 DAXHMX010000000
VERSION     DAXHMX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.190"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>2939
                     /gene="pilC"
                     /locus_tag="ACFM4S_002003"
     CDS             <1..>2939
                     /gene="pilC"
                     /locus_tag="ACFM4S_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6051613.1"
                     /translation="AQAQTQTHKYAIVMNAQNLPEVKWGNDYNKLVQKINEREVTHTS
                     FFRIKKSVSFSFNNKDEVVAEKKDAVVFGAATYLPPYGKVSGFDTVKLTERANALNWI
                     NTTHPGLIGYSYQDSTCSSSGCPEVAYRTQFTFGNSGLAKKKTDNKLDIYEDKSRDNS
                     PIYKVKDYPWLGVSFNLGGESSFKPKRNGSLVSSFSEDVTQQNGAGSQHKDKNLVYTT
                     DDYKSQNNKNHQDKHHAVAFYLNAKLHLLDKKHIKNIVQGKTVNLGILKTRIEPTEAW
                     KRRNSNFFNGSWTYEEKGTVSVKLKLPEVKAGRCINKQNPNKNSKAPSPALTAPALWF
                     GPVQNGKAEMYSASVSTYPDSSSSRIYLQNLKRKTDPGRPGRYSLADLSASDIQSKEP
                     TFTSRQTIIRLDGGVQQIKLDQNKKATGLNGNDGKNETFGIVSEGSFTPDVSEWKKVL
                     LPWTVRASNDDGQFNTFNKEENNGKPKYSQKYRSRDNNKGERNLGDIVNSPIVAVGEY
                     LATSANDGMVHIFKKGNGGDERNYSLKLSYIPGTMPRKDIESKDSTLAKELRAFAEKG
                     YVGDRYGVDGGFVLRQVERNGKDHVFMFGAMGFGGRGAYALDLTKAENGDPTAVSLFD
                     VKNDKNKGNNSAELGYTVGTPQIGKTHDGKYAAFLASGYATKTIDSTDNKTALYVYDL
                     ESSGTLIKKIDVPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGSMYRFDLKDWSVRTI
                     FSGNKPITSAPAISQLKDKRVVIFGTGSDLSEEDVLNTDEQYIYGIFDDDTAASNVDV
                     KLKGLGGGLLEQVLEQKDKTLFLTDYKRSDGSGNKGWVVKLKDGQRVTVKPTVVLRTA
                     FVTIRKYKDNGCGAETAILGINTADGGKLTKKSARPIVPDTNTAVAQYSGHKKGTNGK
                     SIPIGCMQKGNEIVCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPDGKRA
                     GKNNRCF"
BASE COUNT          884 a          712 c          747 g          596 t
ORIGIN      
        1 ggcgcaagcg caaacgcaaa cgcataaata cgctattgta atgaacgcgc aaaatctgcc
       61 tgaggtaaag tgggggaatg actataacaa attggtgcaa aaaatcaatg aacgcgaagt
      121 tacccatacg agttttttca gaatcaaaaa aagcgttagt ttctcattca ataataaaga
      181 tgaagttgtt gctgaaaaaa aagatgctgt cgttttcggc gcggcgacct acctgccgcc
      241 ctacggcaag gtttccggtt ttgataccgt taagctgacc gagcgcgcca atgcccttaa
      301 ttggattaat acgacccacc cggggctgat aggctatagc taccaagata gcacatgctc
      361 cagcagtggt tgtcctgaag ttgcctatag aacccaattt accttcggca attccgggtt
      421 ggcaaaaaag aaaacagaca acaagctgga tatatacgaa gacaaaagcc gcgacaattc
      481 gcccatttat aaagtgaagg attatccttg gttgggcgtg tctttcaatt tgggcggaga
      541 gagctctttc aaaccaaaga gaaacggttc tttggtatct tcttttagcg aggacgtgac
      601 gcagcaaaat ggtgcgggca gccaacacaa agacaaaaac ctcgtttata cgacagacga
      661 ttacaagagt cagaataata aaaaccatca ggacaaacac cacgccgtcg ccttttatct
      721 gaacgccaag ctgcacctgc tggataaaaa acacattaaa aatatcgtgc aaggtaaaac
      781 agttaatttg ggtatcttga aaacacgcat cgagccgacg gaagcatgga aaagacggaa
      841 tagtaacttt tttaacggta gttggacgta tgaagagaaa ggaacagtca gcgtcaaact
      901 caaattgccg gaagtcaaag caggccgctg catcaacaaa cagaacccca ataaaaacag
      961 caaagcccct tcccccgcac tgactgcccc cgcgctgtgg ttcggacctg tgcaaaatgg
     1021 taaggcggag atgtattccg cttcggtttc cacctacccc gacagttcga gcagccgcat
     1081 ctaccttcaa aatctgaaaa gaaaaaccga ccccggcaga cccggccgct attccctcgc
     1141 agacttgagt gcgtcggata ttcaaagtaa agagccgact ttcacaagtc ggcaaacaat
     1201 catccgattg gatggtggcg tgcagcagat caaactggat caaaacaaaa aggccaccgg
     1261 tttaaatgga aatgacggca aaaacgaaac tttcggcatt gttagtgaag ggagcttcac
     1321 gcctgatgtc agcgagtgga aaaaagtgct gctgccttgg acggttcgtg cttccaatga
     1381 tgacggtcaa tttaacacat tcaacaaaga agaaaacaac ggcaagccaa aatacagcca
     1441 aaaataccgc agccgcgaca acaacaaggg cgaacgcaat ttgggcgaca tcgtcaacag
     1501 ccccatcgtg gcggtcggcg agtatttggc tacttccgcc aacgacggga tggtgcatat
     1561 cttcaaaaaa ggcaacggcg gggacgagcg caactatagt ctgaagctca gttatatccc
     1621 gggcacgatg ccgcgcaagg atattgaaag caaagactcc acccttgcca aagagctgcg
     1681 cgcctttgcc gaaaaaggct atgtgggcga ccgctacggc gtggacggcg gctttgtctt
     1741 gcgccaagtc gaacggaatg ggaaagacca tgtgtttatg ttcggcgcga tgggctttgg
     1801 cggcagaggt gcgtatgcct tggatttgac caaagccgaa aacggtgacc cgaccgccgt
     1861 ttccctgttt gatgtcaaaa atgacaaaaa taaaggcaat aatagtgcag aattaggcta
     1921 caccgtcggc acgccgcaaa tcggcaaaac ccacgacggc aaatacgccg ctttcctcgc
     1981 ctccggttat gcgactaaaa ccattgacag cacagacaat aaaaccgcgc tgtatgtgta
     2041 tgatttggaa agcagcggta cgctgattaa aaaaatcgat gtacccggcg gcaagggcgg
     2101 gctttcgtcc cccacgctgg tggataaaga tttggacggc acggtcgata tcgcctatgc
     2161 cggcgatcgc ggcggcagta tgtaccgctt tgatttgaaa gactggtctg tacgcaccat
     2221 tttcagcggc aacaaaccga ttacttccgc gcccgctatt tcccaactga aagacaaacg
     2281 cgtggttatc ttcggcacgg gcagcgattt gagtgaggag gatgtactca atacggacga
     2341 acaatatatt tacggtatct ttgacgacga tacggcggcg agtaatgtag atgtaaaact
     2401 caaaggtttg ggaggcgggt tgctcgagca ggtgcttgag cagaaggata aaaccttatt
     2461 cctgaccgat tataagcgat ccgacggctc gggcaataag ggctgggtag tgaaattgaa
     2521 ggacggacag cgcgttaccg tcaaaccgac cgtggtattg cgtaccgcct ttgtaaccat
     2581 ccgcaaatat aaagacaacg gctgcggcgc ggaaaccgcc attttgggca tcaataccgc
     2641 cgacggcggc aagctgacca agaaaagcgc gcgcccgatt gtgccggaca ccaatacggc
     2701 tgtcgcgcaa tattccggcc ataagaaagg caccaacggc aaatccatcc ctataggttg
     2761 tatgcaaaaa ggcaatgaaa tcgtctgccc gaacggatat gtttacgaca aaccggttaa
     2821 tgtgcgttat ctggatgaaa agaaaacaga cggattttca acaacggcag acggcgatgc
     2881 gggcggcagc ggtatagacc ccgacggcaa gcgtgccggc aaaaacaacc gctgcttct
//
LOCUS       DAXHMX010000191         2913 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHMX010000191 DAXHMX010000000
VERSION     DAXHMX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.191"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..1481
                     /locus_tag="ACFM4S_002004"
     CDS             <1..1481
                     /locus_tag="ACFM4S_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6051614.1"
                     /translation="GTSLAAPYLDKAAENLGTAGKAAVNALGGAAIGYAAGGNVGTAA
                     VGANVDWNNRQLHNSEIRKIKNNAAKFAKLHPGISQRQAEERLVAQAMRQVDAAYYAK
                     HADTDTAAEQFLRSIAGSFKADGETFLDFAPMKYYRDSGKFAEHTGYRDLRAKLGQNP
                     VHTEAQRNRTIEEKTYGTIGRGVGKGIGNTLANNLQAGPPHYVLGITNYSNWGKELIP
                     YENPVERAIGKQAEDMTEKAVAAVSLGRSLPSKVVNVPSKLAKSIKTAETHKPLVQKR
                     TETYFRVEGGGSGNNQAAKAAKNTEKMKAAIDQTHSGVDLPYDSRQIRAKLEKRYGAE
                     NIVSTTIPKKPVQSINNQTKPDYSSIVYTNKSGKVVNVKYKDPISGESKAVNIAYDNR
                     GLPIFDNVSNFTTKIDNSLSYTGQMRAATKDLWKAIESGQVPKNQFTNEQLTKIKAGN
                     EKIPGYTWHHNAQSSPNNMQLIPNSIHTAVRHTGQKSLKKGQ"
     gene            1484..1960
                     /locus_tag="ACFM4S_002005"
     CDS             1484..1960
                     /locus_tag="ACFM4S_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6051615.1"
                     /translation="MRKLSISSDEGLVPIRTIQQFAEDVGVVFPASYVSLLSKHDHLY
                     PKENIFNFVNQYGENDERDISFLGYKQDLGYEDIYSYSCIDDEYGYAKKVIAFGISAN
                     GDYICFDYRKCNENPCIILMYHDDFYEDENGDTKMVTSHIADSFDAFLDMLYELSD"
     gene            2074..>2913
                     /locus_tag="ACFM4S_002006"
     CDS             2074..>2913
                     /locus_tag="ACFM4S_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6051616.1"
                     /translation="MRFSRFEPKGRLKQQIRFQTTFLSTSHHGNQTKAAHRHIHVGST
                     AGTTTIRSGGDTTLKGAQLIGKGIQADTRNLHIESVQDTETYQSKQQNASAQVTVGYG
                     FSASGDYSQSKIRADHASVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSSQSAEDKGK
                     NRFQTATLTASDIQNHSQYKGESFGLGASASISGKTLGQGAQNKPQNKHLTSVADKNG
                     ASSSVGYGSDSDSQSSITKSGINTRNIHITDEAGQLARTGRTAKETEARIYTGIDTET
                     ADQH"
BASE COUNT          992 a          626 c          683 g          612 t
ORIGIN      
        1 gcggcacttc ccttgccgca ccgtatttgg acaaagcggc ggaaaacctc ggtacggcgg
       61 gcaaagcggc ggtcaacgca ctgggcgggg cggcaatcgg ctatgcggcg ggcgggaatg
      121 tcggcacggc ggcagtaggg gcgaatgtcg attggaacaa taggcagctg cacaacagcg
      181 agatcagaaa aattaaaaat aatgccgcta aatttgccaa actccatccc ggcatttccc
      241 aacggcaggc agaagagcgg ctggtggcac aagctatgcg tcaggtcgat gcggcttatt
      301 atgccaaaca tgccgacacc gacacggcgg cggaacaatt cctgcgcagc atagcgggaa
      361 gtttcaaagc cgacggggaa acgttcctgg attttgcccc gatgaagtat tacagggaca
      421 gcggtaaatt tgccgaacat acaggctatc gagatctaag agcaaagttg ggacagaatc
      481 cggtccatac cgaagcgcaa aggaacagaa ctattgaaga aaagacctac ggcacaatag
      541 gcagaggtgt aggtaaaggc atcggcaata ccctagccaa caacctgcag gcgggtccgc
      601 cacactatgt actcggcatt acgaactatt cgaactgggg caaagaactt attccatacg
      661 agaaccctgt tgagcgcgcc atcggcaaac aggcagagga tatgacggaa aaagcggtag
      721 cagctgtgtc tttgggccgg agtttgccgt caaaggttgt taatgtaccc tctaaattag
      781 ctaaaagcat taagacagcg gaaacacata aaccactggt acaaaagcgt acggaaactt
      841 attttagagt tgaaggagga ggaagtggaa ataatcaggc agccaaagcc gctaaaaata
      901 cggaaaaaat gaaagccgca atagatcaaa cccacagtgg agttgacctg ccttatgatt
      961 cgagacaaat tcgagctaaa ctggaaaaaa gatatggagc tgagaatatt gtatccacta
     1021 cgattccaaa gaaaccggtc caaagtatca ataatcaaac taaaccggac tactcatcta
     1081 ttgtatatac taataaaagt ggaaaggtag ttaatgttaa atataaagat cctatatcag
     1141 gggaaagtaa ggctgttaat atcgcctatg ataatagggg attacctata tttgataacg
     1201 tatcaaattt taccaccaaa atagataatt cattgtcgta tacgggacag atgagggcag
     1261 ctactaaaga tttatggaaa gctattgaaa gtggacaagt gcccaaaaat caatttacta
     1321 atgaacaact aacgaagatt aaagcaggaa atgaaaaaat tcctggttat acatggcatc
     1381 ataatgcgca aagctctcct aataatatgc agttaattcc taatagtatt catacagctg
     1441 ttaggcatac tggtcaaaaa tcgttaaaga aaggtcagta aaaatgagaa aactatcaat
     1501 atcatcagat gagggattgg ttccaataag aacaattcaa caatttgctg aagatgtagg
     1561 ggtcgttttt cccgcgtcat atgtatcact tcttagtaaa catgatcatt tataccctaa
     1621 agaaaatatt ttcaattttg tcaatcaata cggtgaaaat gatgaaaggg acatttcatt
     1681 tttagggtat aagcaagatt taggctatga agatatttat agctattcat gtattgatga
     1741 tgaatatggg tatgcaaaaa aagtgattgc ttttgggatt tcagccaatg gcgattatat
     1801 ctgttttgat tacagaaaat gcaatgaaaa tccatgtatc attctgatgt atcatgatga
     1861 tttctatgaa gatgagaatg gggatacgaa aatggtaact agccatattg cagatagttt
     1921 tgatgccttt ttggatatgc tatatgaact ttcagattaa gtgtgtaggt tgagttgaaa
     1981 acccaactaa attaaaaagg tcgtctgaaa tcagcaagcc gtagcccata tgaaacctaa
     2041 actcaacaag taggatgtgt gcggaacgca cgcatgcggt tctcaaggtt tgagcccaaa
     2101 ggtcgtctga aacaacaaat acggtttcag acgacctttc tttcaacaag ccaccacggc
     2161 aatcagacaa aagcagcaca tcgccacatc catgtcggca gtacggccgg cacaaccacc
     2221 atccgcagcg gcggggatac caccctcaaa ggtgcgcagc tcatcggcaa aggcatacag
     2281 gcagatacgc gcaacctgca tatagaaagt gttcaagata ctgaaaccta tcaaagcaaa
     2341 caacaaaacg ccagtgcaca agtgaccgta ggttatggct tcagtgccag tggcgattac
     2401 agccaaagca aaatccgagc cgaccatgct tcggtaaccg agcaaagcgg tatttatgcc
     2461 ggagaagacg gctatcaaat caaggtcgga aaccatacag acctcaaagg cggcatcatc
     2521 accagcagcc aaagcgcaga agacaagggt aaaaaccgct ttcagacggc cacccttact
     2581 gccagcgaca ttcaaaacca cagccaatac aaaggcgaaa gttttggatt gggcgcaagt
     2641 gcgtccataa gcggcaaaac actgggacag ggcgcacaaa ataagcctca aaacaaacac
     2701 ctgacaagcg tggccgataa aaacggcgca agttcatcag tgggttatgg cagcgacagc
     2761 gacagtcaaa gcagcatcac gaaaagcggc atcaacaccc gcaacataca catcaccgac
     2821 gaagcgggac aacttgcccg aacaggcagg actgccaaag aaaccgaagc gcgtatctac
     2881 accggcatcg acaccgaaac tgcggatcaa cac
//
LOCUS       DAXHMX010000192         2866 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHMX010000192 DAXHMX010000000
VERSION     DAXHMX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.192"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..1439
                     /locus_tag="ACFM4S_002007"
     CDS             <1..1439
                     /locus_tag="ACFM4S_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFC6051617.1"
                     /translation="GTSLAAPYLDKAAENLGTAGKAAVNALGGAAIGYATGGSGGAVV
                     GANVDWHNRQLHPSERDWIRKNAQAFAKQEVGGREPTIDEIALAEKRLAQQAAKETDL
                     FWMLTLEKVTDLSAQKFLRQAKGTFTNENGTQQQFFTTQGKQFMRPELFANEVYDLSF
                     YKNHLQSPKNSSISKGTGELARRVTSSTWDKAKQDPILTGLQIAASPVHLVRDVGKAS
                     WQCVSGFGQCLQQGKENFVETGSAIGTGTASFQLNDLKPIYGQDVRGAQAGLVALQTA
                     DGITTALGAAKALNVAGKGSRYFINEWASSPPSGSRAAQRGAVGNLTHSQQVLTDGSH
                     IQRGGKLLPNIRYKAGEFDYLYETDTLGRIKSFETDNLQLTQRDRLKHNSKTPDKVKG
                     QDHAGHLIGDRFGGSPNLDNLVSQHKDVNLSAYKVLENKWAEAIKSGKNVKIQMQIEY
                     AAGTRHPTRFVGYYWIDGKPTRININNL"
     gene            1454..1900
                     /locus_tag="ACFM4S_002008"
     CDS             1454..1900
                     /locus_tag="ACFM4S_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051618.1"
                     /translation="MIFEDYFSELQADMVSICLEYVQGKADKIYIYAAFEPRVSGAGC
                     FFSVKGKLVQRHLVNQEIPEVNLDHQIQVLDILMEDLEKLNSICQKFNQPMPTEIKMI
                     YDVKKNSLSANYQYNLILSSESGLLALPQDVEDAWFEEIKKQMEDN"
     gene            2056..2406
                     /locus_tag="ACFM4S_002009"
     CDS             2056..2406
                     /locus_tag="ACFM4S_002009"
                     /inference="COORDINATES: protein motif:HMM:NF045464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6883 domain-containing protein"
                     /protein_id="HFC6051619.1"
                     /translation="MPTLRLLIPNADSVIIDTRKFTDYALNPNHDVGRHKARVFESAL
                     GYNQSNATELILKIRQGLKDNPARLGKKDQYGQRYTVDIPITGPNGKTAIVTTGWILV
                     PGSEIPKMTTVLVK"
     gene            2416..2607
                     /locus_tag="ACFM4S_002010"
     CDS             2416..2607
                     /locus_tag="ACFM4S_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HFC6051620.1"
                     /translation="MNLNDVVKLKVKQPNSNVPEGSIGTIIEVLQELPSAVFEVEFIN
                     KNGELIDTLTLAECDIEPA"
BASE COUNT          988 a          467 c          633 g          778 t
ORIGIN      
        1 gcggcacttc ccttgctgca ccgtatttgg acaaagcggc ggaaaacctc ggtacggcgg
       61 gcaaagcggc ggtcaacgca ctgggcgggg cggcaatcgg ctatgcaact ggtggtagtg
      121 gtggtgctgt ggtgggtgcg aatgtagatt ggcataatcg gcagttgcat ccgagtgaaa
      181 gagattggat aagaaaaaat gcacaagctt ttgcgaaaca ggaggtaggt ggtagagaac
      241 ccactataga tgaaattgca ttagcagaaa aacgactagc acaacaggcg gctaaagaga
      301 cagatttgtt ttggatgcta acattagaaa aagtaacaga tttatcagct caaaaattcc
      361 taagacaagc aaaaggaact tttacaaatg aaaatggtac gcagcaacaa ttttttacta
      421 cgcaagggaa gcaatttatg cgtcctgaat tgtttgctaa tgaagtatat gatctgtctt
      481 tttacaaaaa ccatttacaa tctcctaaaa atagtagtat cagcaagggt acaggagaat
      541 tggctcgaag agttacttca tctacttggg ataaagcaaa acaagatcct attttgacag
      601 gattgcaaat agctgcctcc cctgtacatt tagttcggga tgtaggtaag gcaagctggc
      661 agtgcgtaag cggctttgga caatgtttgc aacaaggaaa agagaatttt gtagaaacag
      721 gttctgcaat tggaacagga actgcctctt ttcaactaaa cgaccttaag ccgatttatg
      781 gtcaagatgt acgtggtgca caagcaggat tggttgcact acaaacggca gatggaataa
      841 ctacggcatt gggtgcagca aaagcattaa atgttgcagg aaaaggtagt cgctatttta
      901 taaatgaatg ggctagtagt ccgcctagtg gttctcgtgc tgcacagcgt ggggcagtgg
      961 ggaatttaac ccattcgcaa caagttttga cagatggttc acatattcaa cgtggcggaa
     1021 aacttttacc taatattcgc tataaagcag gcgagtttga ttatttatat gaaactgata
     1081 ctttaggtag aattaaaagt tttgaaacag ataatttgca attaacacag agagatagat
     1141 taaagcataa ttcaaaaact cctgataaag tcaaggggca agaccatgca ggacatttaa
     1201 ttggagatag atttggaggt tcgcccaatt tagataattt agtgtcacag cacaaagatg
     1261 taaacttaag tgcgtataaa gttttggaga ataaatgggc ggaagcaatt aaatcaggaa
     1321 aaaatgttaa aatccaaatg caaattgaat atgcagcagg tacaagacac cctacacgat
     1381 ttgttggata ttattggatt gatggtaaac caactagaat taatattaat aatttatgaa
     1441 acaaggattt tttatgattt ttgaagatta ttttagtgaa ttacaagcag atatggtttc
     1501 tatttgtttg gaatatgttc aaggtaaggc tgataaaatt tacatttatg cagcttttga
     1561 gcctcgtgtt agtggtgctg gatgtttttt ctctgttaaa ggaaaacttg tacaacgaca
     1621 tcttgttaat caagaaattc cagaagttaa tcttgaccat caaattcaag tattagacat
     1681 acttatggaa gatttagaaa aactaaattc tatttgtcag aaatttaacc aacctatgcc
     1741 aactgaaatt aaaatgattt atgatgtaaa aaagaatagc ttatcagcaa attatcaata
     1801 taatttaatc ttgtcatctg aatctggatt acttgcttta ccgcaagatg ttgaagatgc
     1861 atggtttgaa gaaattaaga agcaaatgga agataattaa ttcacagcaa gcgtagcaag
     1921 cgtagggtgg gcaactcgtt gcccacgcag ttcaatcgta tatgttaaaa tgatgtttta
     1981 atgtgaaata tttcatttca ggctgcctga aaaaataaaa taacgtggcg tgtatcaaac
     2041 cgcgtgggca acgagttgcc caccctacgc ttgctaatcc ctaacgctga ttctgtcata
     2101 atcgatacca gaaaatttac agattatgca ttgaatccta atcatgatgt tgggagacat
     2161 aaagctagag tttttgaatc agctctagga tataatcaat ctaatgcaac tgagctaatt
     2221 ttgaaaatta gacaaggatt aaaagataac cctgcaagat taggaaaaaa agatcaatat
     2281 gggcaaagat atacagtaga tattcctatt actggaccta atgggaaaac agccattgta
     2341 actacaggct ggattttagt gcctggatct gaaattccca aaatgacaac cgttttagta
     2401 aaataggtgc caataatgaa tttaaatgat gttgtaaagc ttaaagtaaa acagcccaat
     2461 agtaatgtac ctgaaggtag tataggtaca ataatcgaag ttcttcagga attgccatct
     2521 gctgtttttg aagtggaatt tataaataaa aatggcgaac taatcgatac attaacgcta
     2581 gcagaatgtg acatagagcc agcgtaacct gtgcaattaa tgcgaactgt tacatggtag
     2641 ggtgggcaac tcgttgccca cgcagttcag tctcatgtgc taaaactatg ttttaaggcg
     2701 aaagttttgg attgggcgca agtgcgtcca taagcggcaa aacactggga cagggcgcaa
     2761 aaaataaacc tcaaaacaaa cacctgacaa gcgtagccga taaaaacggc gcaagttcat
     2821 cagtgggtta tggcagcgac caagacagtc aaagcagcat cacgaa
//
LOCUS       DAXHMX010000193         2755 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHMX010000193 DAXHMX010000000
VERSION     DAXHMX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.193"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            1..454
                     /locus_tag="ACFM4S_002011"
                     /pseudo
     CDS             1..454
                     /locus_tag="ACFM4S_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(501..1910)
                     /gene="dacB"
                     /locus_tag="ACFM4S_002012"
     CDS             complement(501..1910)
                     /gene="dacB"
                     /locus_tag="ACFM4S_002012"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6051621.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLSHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            2075..2641
                     /locus_tag="ACFM4S_002013"
     CDS             2075..2641
                     /locus_tag="ACFM4S_002013"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6051622.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          667 a          646 c          752 g          690 t
ORIGIN      
        1 caacgcaaag gcaaacgcgg tcgcggtgct gccggtaaag tcgccgtatt cggtcttttg
       61 aagcgaaatg gtaaggttta tacggttaca gtaccgaatt caaaccgcta ctttatttcc
      121 tattatccgt gaacaagtga aacctgacag tattgtttat acggattgtt atcgtagcta
      181 tgatgtatta gatgtgagcg aatttagcca ttttagcttc gctgaaactt cgttttcgta
      241 tcaatcacag cacacatttt gccgaacgac aaaaccatat taatggaatt gagaactttt
      301 ggaaccaggc aaaacgtcat ttacgcaagt ttaacggcat tcccaaagcg cattttgagc
      361 tgtatttaaa ggagtgcgaa tggcgtttta acaacagtga gataaaagtt cttgttccat
      421 tttaaaacaa ttagtaaaat cgagtttgtc ctagttatct aggacagccc cttaaattta
      481 atccactata ttttcctgtt tcaggctcgg cgttctttgc acatcagttt cgcatccagc
      541 cagccatcgc cgccggagat gatgttgttg gcaacgaagt tgtccaagtc gggcagcagg
      601 gaaacggcgc ggccgctgtt gatgatgacg accaccgcca tcggtttgtc gcccagccaa
      661 taacctgcaa gggcgcggac attgttgagc gtgccggttt ttaagcgcaa cagcccgccg
      721 ctttgtttga agcggttgcg taaagtcccg tctgtgccgg cgatgggcag cgtatcgatg
      781 aaatcttgtg caaacgggct gaaataagcc gtttccaaca tttgcgccat cattctcgcc
      841 gttacccttt cttttctaga cagacctgaa ccgttttcca aaaccaaatc cgcaacatcg
      901 atgcccgaca cggcaagttc acgccggacg gcagacgctg cctgttcgga aacggcgggc
      961 agtttgccgt tgccgccgag tttgaggaag acggaacgcg caattagatt gtccgaacgc
     1021 ttgttcatgt ccgtcaaaat ttccttcatc ggtttggcgt gtgcaacggc aagcgtctgc
     1081 gcgccttccg gcgtgtcggc tatgccgata ccgtctgaaa tccgtccgcc gccgagcagc
     1141 cagtggttgg taaaactttg ccggatcagt tcgtcaagcg cgaacatccg gacaccgaca
     1201 ggcttgccca aacagctctc gggaatattg ccgcgcaatt tcagcgtatt gtccgaaaaa
     1261 gatgcacgca tcagtttttt gatcgaaggg caggcagctt gggaggcggt aattttcaag
     1321 ttgttttggg cgaaaatatg cgacaaaggc ggatcggtga ggatgtcggt actgtcggcg
     1381 gcattgcgtt cggcgcgcac cataaccata ccggcagaca gcatagttgg attggggggc
     1441 gtcataaacg gcgaaccgct gtcggcttcg aaatcgtcgg ggctgccgac ttcgccccac
     1501 aggctgtggt cgagcatcag gtgtcccgtg atattgcgta tgccttgttc gcgcaactgg
     1561 cgttggacgg caagcaggtt ttcctgattg aaaacggggt cgccgctgcc cgcccaatac
     1621 aggtttccgt caagcgtgcc gtcgtttacc gtaccgttgc ttttaaactc ggtcgcccag
     1681 cggtaattgc tgccgaaggt tttgaaggcg gcaaacgcgg taacgagttt cattgtggag
     1741 gcggggttga cggggacatc cgagcggtgg tcaatgatga cttttccgct gtcaagctct
     1801 tggacatata cggcgatttc gttttgcgga atgcgacccg tatcgagcgc gtgtgcggcg
     1861 agggaggcga gaagcagcag cagggaggcc gctgttttgg ggaaattcat aggtaaatct
     1921 gttacataaa aaaagcaatt ataaggcaaa gccggataag tgggaacgct gtggcggcgg
     1981 gccggcttgt ttgcagggga aatcacatat ataataatcg ctatcattat gaaatgattg
     2041 agacgcacaa acttaatatt caggaggaat gattatggct aagaaaatca gtattttggt
     2101 gggcagcctg cgccgtgctt cttttgcgcg caaagtggca ttgaatgcgg cggagatgtt
     2161 ccccgaaggc tggcaggcgg aaatcgtcga aatcggacat ctgccgcttt acaatttcga
     2221 ttatgacgac cctgcggtgg aagatgtgcc gctgcccgaa agctacacgg ctttccgcga
     2281 aacgattaag gcttcggacg gcattttgtt cgttacgtcc gaaaataacc gcaccattcc
     2341 cgcctgtttg aaaaatgcgg tggacatcgg ttctaaaccg aatgccgatg tggcttggaa
     2401 aaacaaaccg gccggcatca tcagccattc cgtcggcaag atggggggat acagttcgca
     2461 aaaaaacctg cgccttgcgc tgtcttattt cgatatgccc gtaaccggac agccggaagt
     2521 gtttttgggc aattcgccga cactgtttga tgaaaacggc aaattgattg actcggcaag
     2581 ggattttgtt cagtcatata ttaatcagtt tgtcggtttg attgaaagaa atgccaaata
     2641 aacaaaccaa gtcgaaaagc cgcaaaaccg gttcgggttt tgcggctttt ttatatgggc
     2701 tgggcagcgt caaggtttgc cgtttgccgg ataatgcgcg gcaaatgacc ggcgt
//
LOCUS       DAXHMX010000194         2631 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXHMX010000194 DAXHMX010000000
VERSION     DAXHMX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.194"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            131..973
                     /locus_tag="ACFM4S_002014"
     CDS             131..973
                     /locus_tag="ACFM4S_002014"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6051623.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSVEEGLETARAA
                     GVAV"
     gene            1003..1467
                     /locus_tag="ACFM4S_002015"
     CDS             1003..1467
                     /locus_tag="ACFM4S_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6051624.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            1499..2608
                     /gene="ribD"
                     /locus_tag="ACFM4S_002016"
     CDS             1499..2608
                     /gene="ribD"
                     /locus_tag="ACFM4S_002016"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6051625.1"
                     /translation="MFSETDISMMQNALKLAALGRFSTSPNPRVGCVIAQGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWMPVSTEILGHDIKTVFRKNDNAF"
BASE COUNT          572 a          888 c          655 g          516 t
ORIGIN      
        1 gaaccttaag tttccgtctt tgttttctgt tttcgcggga ataatgggga acaaaccgta
       61 tttcagacgg cattttcagt tcggggtata atccgaatac ttgcgaccat ctgaatcatt
      121 gggacaaact atgtgtcaac tgctgggcat gaactgcaat acgccgaccg atattatgtt
      181 ttcctttgaa ggcttccgcc ggcgcggcgg cattaccgac catcatgccg acggtttcgg
      241 catcggcttt ttcgaaggca aaggcgtgcg cctgttccat gacgacaaac ccagtgccaa
      301 ttcgcccgtc gccgacctcg tgcgcgccta ccagattaaa tcggaaaacg tcgtcgccca
      361 tatccgcaaa gcatcgcaag gacaaacctc gctggcgaac acccatccct ttatgcgcga
      421 aatgtggggt ggctactggc tgtttgccca caacgggcat ttgattgatt ttttccccga
      481 acagggcgaa tttttccacc ccgtcggcac aaccgattcc gaacgcgcgt tctgccacat
      541 cctcaaccgc ctgcgcaccc gctttgccgc ccgtcccgac gacgacacgc tgtttgacgc
      601 gattgcgggg ctgacgcacg aaatccgcaa gttcgggctg tttaacttta tgctttcaga
      661 cggcattgcc ctgtttgccc acgccagcac actgctgcac tacatcgtcc gccaagcccc
      721 gttcggcaag gcgcgcctgc tcgacgacga cgtgatggtc gattttgccg aagtaaccac
      781 gccctccgac cgcgtcgccg ttatcgccac cctgccgctg acccgcgacg aatcatggtc
      841 ccaacttgcc gtggacgaac tggtcatgtt ccgcgaaggc aacatcgtcc gacacgaccg
      901 tcccgaaaac cccgtttata tgagtgtcga agaaggtttg gaaaccgcgc gcgccgccgg
      961 cgtcgccgtc tgaacttcag acgacatagg aggacgaacc cgatgaaatg cccgttttgc
     1021 gcccaccccg acacccgcgt tgccgattcg cgcctgatgg acgaacgcaa cgccgtccgc
     1081 cgccgccgat actgccccaa ctgcggcaaa cgcttcggca cgctcgaaac cgccgaactc
     1141 aaaatgcccg ccgtcatcgg tccggacaaa aaacgttcgc cctttaatgc acaacgcctc
     1201 cgcaacgacc tgaccgccgc cgcccgaaaa tccgccctga cacccgaaca gatcgacgaa
     1261 accgtccgcc tgacggaaca caggctctac acttcgggtc agcgcgacat cccctctgcc
     1321 gcacttgccg acatggtgct aaaagaactg ctccgacaag atacggaagc cgccgtccgt
     1381 ttcgccgccc tgcacaaacg cttcgacaat ccggcagact ttgcctcgtg gctggcgcaa
     1441 gccgtcaaaa cagaccgcaa agcctgattc ccccaaccca tactgatacg gtatccccat
     1501 gttttccgaa acagatattt ctatgatgca aaacgccctc aaacttgccg ctttggggcg
     1561 tttttccact tcgcccaatc cgcgcgtcgg ctgcgttatc gcacaaggca gccaaattgt
     1621 cggacagggc ttccacgtca aagcgggcga accgcacgcc gaagtccacg ccctgcgtca
     1681 ggcgggcgaa atggcacaag gcgcgaccgc ctttgttacc ctcgaaccgt gcagccatta
     1741 cggacgcaca ccgccctgcg ccgaagctct gctccgttcc ggcgtaacac gcgtcgttgc
     1801 cgccatgcgc gaccccaacc cgctggttgc aggcaaaggg cttgccctgc tcgaagcagc
     1861 aggcatcaag acggaatgcg gtttactcga acatcaggca agggaactca accgaggctt
     1921 cctgtcgcgc atcgaacgcc gccgcccctt cgtccgcctc aaatgcgccg tttcgctgga
     1981 cggcaaaacc gccctttcag acggcagcag cttttggatt accggcgaag acgcgcgtgc
     2041 cgacgtacag gttttgcgtg ccgaaagctg cgcggtgctg accggcatcg gcacggtgtt
     2101 ggcggacaat ccccggctca acgtccgcgc ttttccaact ttgcgccaac ccgcacgcat
     2161 cgtgttagac agccgcctgc gcctgccctt ggacagccac cttctgaccg acggacaatc
     2221 tccaacctac atcgccacct tggaacgcga tgaagacaaa ctccgcccct atcgcaaaca
     2281 cgcccacgtc cgcatcctga tgccgtctga aacggcaggc ggcaaaatcg atttgaacag
     2341 cctgatgcgc ctgcttgccg acgaaggcat aggggaactc atagtcgaag caggctccga
     2401 actcacatct gcatttttgg cagaagattt ggcagacgaa atcgtgctgt accgttcgcc
     2461 caaaatcctc ggcagcggca aagacctgtt ttccctgccc gaaaaccgcg ccgccctttc
     2521 cgcaccgccc ttgtggatgc ccgtttccac cgaaatcctc ggacacgaca tcaaaaccgt
     2581 gttccggaaa aacgataacg ccttttaaag ggtttgcgcc gtttcactat a
//
LOCUS       DAXHMX010000195         2613 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXHMX010000195 DAXHMX010000000
VERSION     DAXHMX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.195"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..335
                     /locus_tag="ACFM4S_002017"
     CDS             <1..335
                     /locus_tag="ACFM4S_002017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051626.1"
                     /translation="KTAENLCWGFGCREGKEFCKGLKQKQKPKVPSFPRRRESRPPTR
                     QESIGNNRNRTNLDSRFRGNDGGKVAVFPINSCRSSFLGWREIKQKRAYQKITMQRTV
                     LAILRFAP"
     gene            complement(410..2044)
                     /gene="groL"
                     /locus_tag="ACFM4S_002018"
     CDS             complement(410..2044)
                     /gene="groL"
                     /locus_tag="ACFM4S_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6051627.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLEDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(2137..2427)
                     /gene="groES"
                     /locus_tag="ACFM4S_002019"
     CDS             complement(2137..2427)
                     /gene="groES"
                     /locus_tag="ACFM4S_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6051628.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGAHRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          534 a          696 c          690 g          693 t
ORIGIN      
        1 cgaaaacagc cgaaaacctg tgttggggtt tcggctgtcg ggagggaaag gaattttgca
       61 aaggtctcaa acaaaaacag aaacctaaag tcccgtcatt cccgcgcagg cgggaatcca
      121 gaccaccaac gcggcaggaa tctatcggaa ataaccgaaa ccggacgaac ctagattccc
      181 gctttcgcgg gaatgacggc ggaaaggttg ctgtttttcc gataaattcc tgccgctctt
      241 cgtttttggg atggcgggaa ataaaacaaa agcgcgcgta tcaaaaaata acaatgcaaa
      301 gaacggtgtt ggcaatttta aggtttgcgc cttagccgca cttattcgca aaaaaaccgc
      361 acggcgttga ccgtgcggct tgttgtctga aggcttcaga cggcattgct tacatcatgc
      421 cgcccatacc acccatgccg cccatatcag gcatagccgg tttgtcttca gggatttcag
      481 caatcatgca gtctgtggtc agcatcaggc cggcgataga cgcggcgtgt tgcagcgcgg
      541 aacgggttac tttggcgggg tcgagtacgc ccatttcgat catgtcgccg tattcgccgc
      601 tgccagcgtt gtaaccatag ttgcctttgc cttccaacac tttgttcaca accacgctgg
      661 gttcgccgcc tgcgttggca acgatttggc gcagcggaga ctcaacggcg cgcaagacga
      721 tttgtacgcc tgcgtcttgg tctgcattgc cggtgtgcag gttttccaga gcggcacggg
      781 cgcgcaacag ggctacgccg ccgcctgcga ccacgccttc ttcaacggct gcgcgggtag
      841 cgtgcagcgc gtcttccacg cggtcttttt tctctttcat ttcgacttcg gtagcggcgc
      901 cgactttgat cactgccacg ccgcctgcca atttagccac gcgctcttgc agtttttctt
      961 tgtcgtaatc gctggttgcg gtttcgattt gttggcggat ttcggcaaca cgcgcttcga
     1021 tttgggctgc gtcgccaaag ccgtcgatga tggtggtgtt ttctttaccg atttcgatgc
     1081 gtttggcttg acccaagtct tccaaagtcg ctttttccag agacaggccg acttcttcgg
     1141 aaatcaccac gccgccggtc aggatagcga tgtcttgcag catcgctttg cggcggtcgc
     1201 cgaagcccgg agctttgacg gcaacggttt tcaggatgcc tcggatgttg ttcaccacca
     1261 aagtcgccaa ggcttcgcct tctacgtctt cagcgataat caacagcgga cggctggctt
     1321 tcgccacttg ttccaaaaca ggcagcaggt cgcggatgtt gctgattttt ttgtcaaaca
     1381 gcaatacaaa cggattgtcc aaagcagcga tttgtttttc agcatcgttg atgaagtaag
     1441 gggacaggta gccgcggtcg aactgcatac cttcaaccac gtccagctcg ttttccaaag
     1501 atttgccgtc ttcaacggta atcacgcctt ctttgccgac tttttccatc gcttcggcga
     1561 taatcgcgcc gacttgttcg tcagagttgg cggaaatcga gccgacttgg gcgatttctt
     1621 tggaagtatc gcaaggtttg gcgatgtttt tcagctcgtc aaccaaagcg gcgacggctt
     1681 tgtcgatacc gcgtttcagg tcggtcggat tcatacctgc ggtaacatat ttcatgcctt
     1741 cggcaacgat ggattgtgcc agtacggtgg cggtagtcgt accgtcgccc gccacgtcgt
     1801 tggttttgga cgcaacttct ttcaccattt gcgcgcccat attttcaaac ttgtctttca
     1861 gttcgatttc tttggcgacg gttacgccgt ctttggtgat gtgcgggccg ccgaaagcgc
     1921 ggtcaaccac tacgttgcgg cctttgggac ccagggttac gcgcacggcg tttgccaaaa
     1981 tgttcacgcc gtttaccatt ttttggcgga cttcattgcc gaactgtacg tcttttgctg
     2041 ccatttcaat tctccaaaaa tcattaaaac tgtctgataa aaccgtttat gccgtctgaa
     2101 ggcggtttgc cgtttcagac ggcatcgtgt ccatatttat ttttcaacga tgccgaagat
     2161 gtcttcttcg cgcatcacca acagctcttc gccgtcggct ttgacggttt ggccgctgta
     2221 tttgccgaag atgattttgt cgccgacttt gacatccagc ggacggtgcg cgccgtcttt
     2281 accgattttg cccgcgccca cggcgatgac ttcgcccata tcgggttttt cggcggccgc
     2341 acccggcaaa acgatacccg atgcggtttt ttcttcagct tccaagcgtt tgacgacgac
     2401 gcggtcgtgt aaaggacgga tggtcatatt tatgctccga taaaatggtt tgaaaacaat
     2461 catctgcccg aacggttcgg acagattgaa gtggaaaccg ggctgccgcc tgccggccgc
     2521 ccgtatgaat cggcaaaatt agggtgtgtg cgggcaaatt caagtgaggc ggaaaaaatt
     2581 tatttccgcc gttttttata gtggactaaa ttt
//
LOCUS       DAXHMX010000196         2613 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXHMX010000196 DAXHMX010000000
VERSION     DAXHMX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.196"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(311..544)
                     /locus_tag="ACFM4S_002020"
     CDS             complement(311..544)
                     /locus_tag="ACFM4S_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6051629.1"
                     /translation="MKLTLMFREYCSLCHKMRDELKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(710..910)
                     /locus_tag="ACFM4S_002021"
     CDS             complement(710..910)
                     /locus_tag="ACFM4S_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6051630.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1082..1957)
                     /gene="xerD"
                     /locus_tag="ACFM4S_002022"
     CDS             complement(1082..1957)
                     /gene="xerD"
                     /locus_tag="ACFM4S_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6051631.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2009..2449)
                     /locus_tag="ACFM4S_002023"
     CDS             complement(2009..2449)
                     /locus_tag="ACFM4S_002023"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6051632.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
BASE COUNT          698 a          745 c          522 g          648 t
ORIGIN      
        1 taccgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagtaaaaca
       61 tgatatgatt gccaacaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggtcaat atgcaacacc
      181 gtagaagatt gacaatttac caggcatcca aacgtgcttc ctttactggc aggtcatccg
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgtctgaagt cctgcttcag
      301 acggcatttt ttaccgttcg agaaactgtt tcaacctgtc ctcatccaaa aaccagtggc
      361 agatttcctc atctcccgcc aacaaaacgg gaaccagttc attgtatttt tcttccaaaa
      421 caggattttc atccacatcg accacttcca gcccgaaccc gtattcatcc tgaaaaggtt
      481 tgagttcgtc gcgcattttg tggcacaagc tgcaatattc acgaaacatc aaggtcaatt
      541 tcatccgcgt ttccttatct gtcaatttgc acacgccaaa gccttagacg cagcagaatc
      601 atggtctatt tgggaaaaaa caatgttttc aaggaagatg atactcaagt cctgccaaaa
      661 acagcaaaaa tgccgtctga acagttcaga cggcatttcc aaaaccgttt tacgcttgaa
      721 cgttgatacc cgccgtaacc gtcggttgcg cattgtgcgc cgtcagaaac gaagcggcaa
      781 gctccccgca gcagccgcag cagctcgcta cgcccaattg cgactgcaaa tcgcccattg
      841 tggtcgcgcc ggcggcgatg gtttccttga tttgatggtc ggtaacggca ttgcagatgc
      901 agacaaacat tttgtgctcc gtgtgttctc aaactatcgt accgatagcg gcttttatta
      961 tcgtatgcaa atataaataa aaacggttcg cattgcaagg tcggcataca cggtttgtct
     1021 tggtaatttt ttatcaagtt ttcatttccg attgatttct tattaaaccg aagcagaagc
     1081 ctcagtttcg ggaatggtgt tccttcacta caccctgcaa ccatacattg gcaacatggg
     1141 tgtaaatctg cgtcgtattc aaatcggcat gtcccaacat atcctgaacc acgcgcaaat
     1201 ccaatccgtg ctgcaccaag tgcgtggcaa aggcgtggcg caggctgtgc gggctgatgt
     1261 gcccgatgcc tgcctgactt gcatattctt tgacaatcat ccatgccaac tgacgagaaa
     1321 tgcccgtctt tttctgactg acaaacaatg cgtcgcaatt cctgcctttc agcagaagtg
     1381 ggcgtgcctc cgtataatag cgttccaccc aatacgccga ctcctgcccc atcgggacca
     1441 tcctctgctt atcgcccttg cccagcgcgg taatacagcc cctgtccaaa tccacattgc
     1501 cgaagttcag cccgaccgcc tcgctgacgc gcaagccggt cgcgtacatc aattcgagca
     1561 gtgccttgtc ccgcaaaccg tgtggcgtat cagtatccgg agtggcaagc agccgggaaa
     1621 tctgctgttc tgtaatcagt gtcggaatat tcttgtcgat tttgggcggt ttcagcaaac
     1681 gggtgggatt gtccgtcctt atgccttcac gctccatcca tatatacagg cgtttgcacg
     1741 ccgataatgc gcgcgcctgc gaactccgtt gctctccgtc aacataaacc gccgccgcca
     1801 aatccgcttc gtccgcatcc ttcagcattc tgcccgattg ggacaggcgg cgggcgattt
     1861 tttccaaatc gcgccggtaa ccgtttaaag tattctgact gagccgcctg tccaaccaca
     1921 gcgtttcaag cagcctgtcg atcaaacctt cttccatacc gttccaaaca aatgccgtct
     1981 gaatcttctt cagacggcat ggttcacatt atcgggaaag cgtttccaac acttcctgtg
     2041 catgacccgc cactttgact ttccgccatt catgggcgat ttctccatcc ttattcaaga
     2101 cgaacgtact gcgctcgata cctaacgact ctttcccgta cagtttcttc aatttgatga
     2161 catcaaacag gcggcacact gtttcatcct tgtcgctcaa cagctcgaac cggaaaccct
     2221 gcttggcgca aaaattctga tgcgccttta cgccgtcgcg ggaaataccg accacggtat
     2281 aacccaatgc ctcaaactgt tccaaacgcg cattgaaatc caagccttcc gtcgtacagc
     2341 ccggcgtact gtctttcgga taaaaataca cgaccaaagg cagatgttct gccgaatgaa
     2401 aatccgcacc gctgctcgaa ggcagggtaa attcatattt cacatccata gtcctactcc
     2461 cgatattccc attattcaaa acggcacgca gacgcaccgc cgcaattgcc aaaccaaccc
     2521 cgattctacc gccccaaagg acaaggattc aaccgccgga aacatccaaa ccgacacacg
     2581 acggcatgaa aaatatccat gtcaaaccac aaa
//
LOCUS       DAXHMX010000197         2598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXHMX010000197 DAXHMX010000000
VERSION     DAXHMX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.197"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(214..285)
                     /locus_tag="ACFM4S_002024"
     tRNA            complement(214..285)
                     /locus_tag="ACFM4S_002024"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:cct)
     gene            complement(305..751)
                     /locus_tag="ACFM4S_002025"
     CDS             complement(305..751)
                     /locus_tag="ACFM4S_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6051633.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALIQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(756..1730)
                     /gene="ispB"
                     /locus_tag="ACFM4S_002026"
     CDS             complement(756..1730)
                     /gene="ispB"
                     /locus_tag="ACFM4S_002026"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6051634.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1954..2262
                     /gene="rplU"
                     /locus_tag="ACFM4S_002027"
     CDS             1954..2262
                     /gene="rplU"
                     /locus_tag="ACFM4S_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6051635.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2287..2559
                     /gene="rpmA"
                     /locus_tag="ACFM4S_002028"
     CDS             2287..2559
                     /gene="rpmA"
                     /locus_tag="ACFM4S_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6051636.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          764 a          696 c          575 g          563 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacggagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa
      121 gttttcagat ggcaaaaaag ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc
      181 aacacgggaa acagacaaat gcaaatcgtc aaacctcgcc aacaggaatc gaacctgtat
      241 tttacgctta ggaggcatac gttctatccg ttgaactatg gcgagccgaa atgaaaagga
      301 gattttaacc ctttccgacg acaaaagaca agatgccggc cgcaatcagc ggcccgacag
      361 ggataccgcc cataaatgcc accccgataa ccgtcccgat taacagccct gtaattaaaa
      421 caggctgctg tcccatcaaa ggcacgccgc gtcccgccag ccaagccacg aaaataccga
      481 taaaaacggc ggatatcatt ttaaaattca aaaattcggc aacgggagga acctgcgcct
      541 ttcctgaaac caacggactc aaaaccccta tggtcaaaag aatgataccg agattcaacc
      601 catgcttctc gaccaacggg acaaactgta tcaatgccgt ctgctgcatc agcagcaata
      661 tggttgccga gatggtaatc gaattgttgt tgctgaccac ccccaacaga atcagcgtaa
      721 ccagaaacag gggaacaaaa ctgaaattca tcgcctcaag acaccctgac caaagattcc
      781 ttcgccagct gaatcatggc atccttcact tcgctgtcgg gcagggcatc caaggcggca
      841 acggcacaat cgactgcttt gcgcgcctcg cctattgaat atgccaaagc atccgaacgg
      901 acgacataat cgtggatttt ctcaaaatag ctgcgatctg cattttccaa agcagtacgc
      961 acatcgttcg caacctgttc ggaaccctga cgcatcagat aaatcaaagg caaagtcggt
     1021 tttccttccg ccaaatcgtc gccgacgttt ttgccggttt cttcggtttc gccagaatag
     1081 tccagcacat cgtcaataat ttggaatgcc gtaccgacat acataccgta gtctttcaac
     1141 gcccgttcgt gttcggggga agccttgccc aaaattgcgc cgacttgagc ggcagcttca
     1201 aacaattttg ccgttttata ttggatgact tggatatatt gttcttcggt aatgtccgta
     1261 ttgccgatgt tcatcagctg catgacttcg ccctcggcaa taatgttggt tgcatccgcc
     1321 atcacttcca aaacgcgcat actgcccgag gcaaccatca gttgaaaggc gcgcgtgtat
     1381 aaaaagtcgc caaccaacac tgccgccgca ttgccgaaca gattgtttgc cgttgcccgc
     1441 ccacggcgca aatcgctttc atcgacgaca tcgtcgtgca ggagggtgga agtgtggata
     1501 aactcgacca tcgccgccag cgaatacagt ttctcgtcat cataaccgac cgccttaccc
     1561 gccaaaatcg tcataatcgg acgcaggcgt ttgccgcccg cgctgatgat atatgtaccg
     1621 atttgcgaaa tcagtgcgac atcggattgc accgcacggt tgatgacttc attgactttg
     1681 gcaaggtctt caggcagatg tcgctggaaa tagggcagat tctcgagcat ggcagactct
     1741 caggtttacg tttccgtcaa acggcagata ccgcttgaaa gctgattcaa atagcgggca
     1801 attatatcag caaagctatg aggacacatc ttttcagacg gcatatggtt atccgaaccg
     1861 cgcaaacttt gacaaaacag gcaaataaca atacaatgcc gagttccgca caatggtgtg
     1921 cggatattaa ccataaccct tatggagttg agtatgtacg cggtcgtaaa aaccggcggc
     1981 aaacagtata aagtttccgt cggcgaaaaa ttgaaagtag aacagatacc agccgaactc
     2041 gacagccaaa tcgaactgac cgaagttttg atgattgctg acggcgaatc tgtaaaagtt
     2101 ggcgcaccct ttatcgaagg tgcaaaagta acggctaaag tagtggcaca cggtcgtggc
     2161 gaaaaagtcc gcatcttcaa aatgcgccgc cgcaaacact accaaaaacg ccaaggccac
     2221 cgccaaaatt tcacccaaat cgaaatcgtg gcaatcgcct aaccttaaaa cttaggagca
     2281 ttacaaatgg caagtaaaaa agcaggcggc agcacccgca acggtcgcga ttcagaagcc
     2341 aaacgcttgg gcgttaaagc ctacggcaac gagctgattc ccgcaggttc catcatcgta
     2401 cgccaacgcg gtaccaaatt ccacgcaggc gacaacgtag gtatgggcaa agaccacact
     2461 ttgttcgcca aagttgacgg ttatgtcgaa ttcaaaacca aaggcgcgct gaaccgtaaa
     2521 actgtcagca tccgtcctta caccggttct gaagaataat ccaaccgata cctgaagccg
     2581 catcttttca cgatgcgg
//
LOCUS       DAXHMX010000198         2596 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXHMX010000198 DAXHMX010000000
VERSION     DAXHMX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.198"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            1..2429
                     /locus_tag="ACFM4S_002029"
                     /pseudo
     CDS             1..2429
                     /locus_tag="ACFM4S_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242392.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
BASE COUNT          749 a          590 c          616 g          641 t
ORIGIN      
        1 acggcgacgg catagaaaca gtcgccgcca aaggcttttc aggcagcctc ttcgaccata
       61 acggcaacgg catccgcacc gccactggct gggtttctgc cgatgacggt ttactcgtcc
      121 gcgatttgaa cggcaacggc atcatcgaca acggcgcgga actcttcggc gacaacacca
      181 aactggcaga cggttctttt gccaaacacg gctacgcagc tttggccgaa ttggattcaa
      241 acggcgacaa catcatcaac gcggcagacg ccgcattcca atccctgcgt gtatggcagg
      301 atctcaacca ggacggcatt tcccaaacca acgaactccg cacccttgaa gaattgggta
      361 ttcaatcttt ggatctcgcc tataaagatg taaataaaaa tctcggtaac ggtaacactt
      421 tggctcagca aggcagctac accaaaacaa acggtacaac cgcaaaaatg ggggatttac
      481 ttttagcagc cgacaacctg cacagccgct tcaaagacaa agtggaactc actgccgaac
      541 aggcaaaagc cgccaatctt gcgggcattg gtcgtctgcg cgatttgcgc gaagctgccg
      601 cattgtccgg cgatttggcc aatatgctga aagcttattc tgccgccgaa actaaagaag
      661 cacagttggc attgttagat aatttgattc acaaatgggc ggaaaccgat tcgaactggg
      721 gcaaaaaatc gccaatgcga ctttcaaccg attggacgca aacggctaat gaaggtattg
      781 cactgacacc atcccaagta gcacaactaa aaaagaacgc tttagtttcc ctttctgata
      841 aagctaaagc aactattgac gccgcccgcg accgcattgc cgtgcttgat gcctacacgg
      901 ggcaggattc caacacactc tattacatga gcgaggaaga tgcgcttaat atcgtcaaag
      961 taaccaacga tacatacgac catctcgcca aaaacatcta ccaaaacctg ttattccaaa
     1021 cccgtttgca gccatatttg aatcaaatca gtttcaaaat ggaaaatgat acgttcactt
     1081 tggattttag tggtcttgtt caagcattta accatgtcaa agaaactaat ccgcaaaaag
     1141 cttttgtgga tttggccgag atgcttgcat atggcgaact tcgttcttgg tatgaaggcc
     1201 gaagactaat ggccgattat gtggaggagg caaaaaaagc aggtaaattt gaagattacc
     1261 agaaagtgtt gggtcaggag accgttgcat tattagctaa aacatcgggt acgcaagcag
     1321 atgatatcct gcaaaatgta ggctttggtc ataataaaaa tgtttcttta tatggtaatg
     1381 acggcaacga cactctgatc ggcggtgcag gcaatgatta cttggagggc ggcagcggtt
     1441 cggatactta tgtcttcggc aaaggcttcg gtcaggatac ggtctataat tacgactacg
     1501 ctaccggacg caaagacatc atccgcttta ccgacggtat tacagccgat atgctgactt
     1561 ttacccgaga gggcaaccat cttcttatca aggcaaaaga cggcagtgga caagtgactg
     1621 ttcagtccta tttctagaac gatggctcag gtgcttaccg tatcgatgag attcatttcg
     1681 ataacggcaa agtactggat gttgccactg tcaaaaaact ggtacagcaa tccaccgacg
     1741 gttcggacag attgtatgcc taccaatccg gaagtacctt aaatggcgga ttgggcgatg
     1801 actatctgta cggtgccgac gggaatgacc tgctgaatgg tgatgcaggc aacgacagta
     1861 tctacagtgg caatggcaat gatacgctca atggaggaga aggcaacgac gccctgtacg
     1921 gctataatgg taacgatgca ctgaatggtg gcgaaggcaa tgatcatttg aacggcgaag
     1981 acggtaacga cactctgatc ggcggtgccg gtaatgatta cttggagggc ggcagcggtt
     2041 cggatactta tgtcttcggc gaaggcttcg gtcaggatac ggtctataat taccatgtgg
     2101 ataaaaactc tgacactatg cactttaaag gatttaaagc agcagatgtt cattttatcc
     2161 gttccggaag tgatttggtg cttagcgctt ctgaacaaga caacgtacgt atttccggat
     2221 tcttctatgg tgaaaaccat cgtgtagata catttgtctt tgatgatgca gctatcagta
     2281 atccagattt tgccaagtat attaatgctg gcaataattt ggtacagtct atgtctgtgt
     2341 tcggttctaa tactgctgcg acaggaggaa atgtggatgc caatatacaa tccgtacagc
     2401 agccgttatt ggtaacgcca tctgcataag gagcctaatc acattcatgg cttaaactga
     2461 aaaacagcaa tcaagtttat tttgattgct gtttttctta atattgggat aagggtcgta
     2521 ttttaattaa ccttaatcgg tgcacttcta gcaatatagt ggattcacaa aaaccagtac
     2581 agcgttgcct cgcctt
//
LOCUS       DAXHMX010000199         2582 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXHMX010000199 DAXHMX010000000
VERSION     DAXHMX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.199"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            77..2227
                     /gene="yccS"
                     /locus_tag="ACFM4S_002030"
     CDS             77..2227
                     /gene="yccS"
                     /locus_tag="ACFM4S_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6051637.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLYDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
     gene            complement(2386..>2582)
                     /locus_tag="ACFM4S_002031"
     CDS             complement(2386..>2582)
                     /locus_tag="ACFM4S_002031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051638.1"
                     /translation="RRESRPWDNSNIQRLAEALEILDSHFRGNDGMQVRGNDGILGFC
                     FWFSVFVGMTECRFVGMTGF"
BASE COUNT          630 a          982 c          504 g          466 t
ORIGIN      
        1 agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actatattca cttcataaca
       61 ataaaccggt aaaaccatga aaaccccact cctcaagcct ctgctcatta cctcgcttcc
      121 cgttttcgcc agtgttttta ccgccgcctc catcgtctgg cagctaggcg aacccaagct
      181 cgccatgccc ttcgtactcg gcatcatcgc cggcggcctt gtcgatttgg acaaccgcct
      241 gaccggacgg ctgaaaaaca tcatcgccac cgtcgccctg tttaccctct cctcgctcac
      301 ggcacaaagc accctcggca cagggctgcc cttcatcctc gccatgaccc tgatgacctt
      361 cggctttacc attttaggcg cggtcgggct gaaataccgc accttcgcct tcggcgcact
      421 cgccgtcgcc acctacacca cacttaccta cacccccgaa acctactggc tgaccaaccc
      481 cttcatgatt ttatgcggca ccgtactgta cagcaccgcc atcatcctgt tccaaatcat
      541 cctgccccac cgccccgtcc aagaaagcgt cgccaacgcc tacgacgcac tcggcggcta
      601 cctcgaagcc aaagccgact tcttcgaccc cgatgaggca gcctggatag gcaaccgcca
      661 catcgacctc gccatgagca acaccggcgt catcaccgcc ttcaaccaat gccgttccgc
      721 cctgttttac cgtttgcgcg gcaaacaccg ccacccgcgc accgccaaaa tgctgcgcta
      781 ctacttcgcc gcccaagaca tccacgaacg catcagctcc gcccacgtcg actaccaaga
      841 gatgtccgaa aaattcaaaa acaccgacat catcttccgc atccaccgcc tgctcgaaat
      901 gcagggacaa gcctgccgca ataccgccca agccctgcgc gcaagcaaag actacgttta
      961 cagcaaacgc ctcggccgcg ccatcgaagg ctgccgccaa tcgctgcgtc tcctttacga
     1021 cggcaacgac agccccgaca tccgccacct gcgccgtctt ctcgacaacc tcggcagcgt
     1081 cgaccaacaa ttccgccaac tccagcacaa cggcctgcag gcagaaaacg accgcatggg
     1141 cgacacccgc atcgccgccc tcgaaaccgg cagcctcaaa aacacctggc aggcaatccg
     1201 tccgcagcta aacctcgaat caggcgtatt ccgccatgcc gtccgcctgt cccttgtcgt
     1261 tgccgccgcc tgcaccatcg tcgaagccct caacctcaac ctcggctact ggatactact
     1321 gaccgccctt ttcgtctgcc aacccaacta caccgccacc aaaagccgcg tccgccagcg
     1381 catcgccggc accgtactcg gcgtaatcgt cggctcgctc gtcccctact tcaccccgtc
     1441 tgtcgaaacc aaactctgga ttgtcatcgc cagtaccacc ctctttttca tgacccgcac
     1501 ctacaaatac agtttctcca ccttcttcat taccattcaa gccctgacca gcctctccct
     1561 cgcaggtttg gacgtatacg ccgccatgcc cgtacgcatc atcgacacca ttatcggcgc
     1621 atcccttgcc tgggcggcag tcagctacct gtggccagac tggaaatacc tcacgctcga
     1681 acgcaccgcc gcccttgccg tatgcagcaa cggtgcctat ctcgaaaaaa tcaccgaacg
     1741 cctcaaaagc ggcgaaaccg gcgacgacgt cgaataccgc gccacccgcc gccgcgccca
     1801 cgaacacacc gccgccctca gcagcaccct ttccgacatg agcagcgaac ccgcaaaatt
     1861 cgccgacagc ctgcaacccg gctttaccct gctcaaaacc ggctacgccc tgaccggcta
     1921 catctccgcc ctcggcgcat accgcagcga aatgcacgaa gaatgcagcc ccgactttac
     1981 cgcacagttc cacctcgccg ccgaacacac cgcccacatc ttccaacacc tgcccgaaac
     2041 cgaacccgac gactttcaga cggcattgga tacactgcgt ggcgaactcg acaccctccg
     2101 cacccacagt agcggaacac aaagccacat cctcctccaa cagctccaac tcatcgcccg
     2161 acagctcgaa ccctactacc gcgcctaccg ccaaattccg cacaggcagc cccaaaacgc
     2221 cgcctgaaaa agtttcggca ttttgtaaga gagagcagat tgtcagacag gttgcaagat
     2281 agtggataaa gctttgcgca gggtaaatgc gtagcaactg aaccgtcatt cccacgaatc
     2341 tacattccgt cattcccacg aaaacagaaa accaaaaaca gaaacctaaa atcccgtcat
     2401 tcccacgaac ctacattccg tcattcccac gaaaacagaa aaccaaaaac agaaacctaa
     2461 aatcccgtca ttcccacgaa cctgcatccc gtcattccca cgaaagtggg aatccagaat
     2521 ctctaaagct tcagctaacc tttgaatatt actgttatcc caaggtctag attcccgcct
     2581 gc
//
LOCUS       DAXHMX010000200         2557 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXHMX010000200 DAXHMX010000000
VERSION     DAXHMX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.200"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            351..2495
                     /gene="fetA"
                     /locus_tag="ACFM4S_002032"
                     /gene_synonym="frpB"
     CDS             351..2495
                     /gene="fetA"
                     /locus_tag="ACFM4S_002032"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6051639.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNNEKDYEAGKGFRNNFNGGK
                     TVPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFTVGSEDSRINIK
                     RQAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPN
                     HTRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSQFKIEDKEKATDEEKN
                     KNRENEKIAKAYRLTNPTKTDAGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSS
                     SNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARN
                     TEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGAS
                     YRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFQNPNLEIG
                     WRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDIFANWKPLGKDTLNVNLSVNN
                     VFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          733 a          734 c          571 g          519 t
ORIGIN      
        1 gacgcggaaa ggttgctttt cgtcattccc gcatttgtta atccactata tttccgccgt
       61 tttttatagt ggactaacaa aaaccagtac agcgttgcct cgccttgccg tactatctgt
      121 actgtctgcg gcttcgtcgc cttatcctga tttttgttag tccactatac atttccgaca
      181 aaacctgtca acaaaaaaca acacttcgca aataaaaacg ataatcagct ttgcaaaaat
      241 cccccccccc tgttaatata aataaaaata attattaatt atttttctta tcctgccaaa
      301 tcttaacggt ttggcttaac ttcccttcat acactcaaga ggacgattga atgaataccc
      361 cattgttccg tctcagcctg ctctcgctta ccctagcggc aggttttgcc catgcggcag
      421 aaaataatgc caaggtcgta ctggataccg ttaccgtaaa aggcgaccgc caaggcagca
      481 aaatccgtac caacatcgtt acgcttcaac aaaaagacga aagcaccgca accgatatgc
      541 gcgaactctt aaaagaagag ccatccatcg atttcggcgg cggcaacggc acgtcccaat
      601 tcctgacgct gcgcggcatg ggtcagaact ctgtcgacat caaggtggac aacgcctatt
      661 ccgacagcca aatcctttac caccaaggca gatttattgt cgatcccgct ttggttaaag
      721 tcgtttccgt acaaaaaggc gcgggttctg cctctgccgg tatcggcgca accaacggcg
      781 cgattatcgc caaaaccgtc gatgcccaag acctgctcaa aggcttggat aaaaactggg
      841 gcgtgcgcct caacagcggc ttcgccagca acgaaggcgt aagctacggc gcaagcgtat
      901 tcggaaaaga gggcaacttc gacggcttgt tctcttacaa ccgcaacaat gaaaaagatt
      961 acgaagcagg taaaggcttc cgtaataatt tcaacggcgg caaaaccgta ccgtacagcg
     1021 cgctggacaa acgcagctac ctcgccaaaa tcggaacaac cttcggcgac ggcgaccacc
     1081 gcatcgtgtt gagccatatg aaagaccaac accgaggcat ccgcactgtg cgtgaagaat
     1141 ttaccgtcgg cagcgaagat tcacggataa atattaaacg ccaagcccct gcttaccgcg
     1201 aaaccacaca atccaacacc aatttggcgt acacgggtaa aaacctgggc tttgtcgaaa
     1261 aactggatgc caacgcctat gtgttggaaa aagaacgcta ttccgccgat gacagcggca
     1321 ccggctacgc aggcaatgta aaaggcccca accatacccg aatcaccact cgtggtgcga
     1381 acttcaactt cgacagccgc cttgccgaac aaaccctgct gaaatacggt atcaactacc
     1441 gccatcagga aatcaaaccg caagcgtttt tgaattcaca atttaaaatt gaagataaag
     1501 aaaaagcaac tgatgaagag aaaaataaga accgtgaaaa tgaaaaaatt gccaaagcct
     1561 accgtctgac caacccgacc aaaaccgatg ccggcgcgta tatcgaagcc attcacgaga
     1621 ttgacggctt taccctgacc ggcgggctgc gttacgaccg cttcaaggtg aaaacccacg
     1681 acggcaaaac cgtttcaagc agcaacctta acccgagttt cggcgtgatt tggcagccgc
     1741 acgaacactg gagcttcagc gcaagccaca actacgccag ccgcagcccg cgcctgtatg
     1801 acgcgctgca aacccacggc aaacgcggca tcatctcgat tgccgacggc accaaagccg
     1861 aacgcgcgcg caataccgaa atcggcttca actacaacga cggcacgttt gccgccaacg
     1921 gcagctactt ctggcagacc atcaaagacg cgcttgccaa tccgcaaaac cgccacgact
     1981 ctgtcgccgt ccgtgaagcc gtcaacgccg gctacatcaa aaaccacggt tacgaattgg
     2041 gcgcgtccta ccgcaccggc ggcttgactg ccaaagtcgg cgtaagccac agcaaaccgc
     2101 gcttttacga tacgcacaaa gacaagctgt tgagcgcgaa tcctgaattt ggcgcacaag
     2161 tcggccgcac ttggacggct tcccttgcct accgcttcca aaacccgaat ctggaaatcg
     2221 gctggcgcgg ccgttatgtt caaaaagccg tgggttcgat attggtggca ggtcaaaaag
     2281 accgcaacgg caaattggaa aacgttgtac gcaaaggttt cggtgtgaac gacatcttcg
     2341 ccaactggaa accgctgggc aaagacacgc tcaatgttaa tctttcggtt aacaacgtgt
     2401 tcaacacgtt ctactatccg cacagccaac gctggaccaa taccctgccg ggcgtgggac
     2461 gtgatgtacg cttgggcgtg aactacaagt tctaaaacgc acatcccgaa aaaatgccgt
     2521 ctgaaagcct ttcagacggc atctgttctg ataattt
//
LOCUS       DAXHMX010000201         2470 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXHMX010000201 DAXHMX010000000
VERSION     DAXHMX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.201"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(13..819)
                     /locus_tag="ACFM4S_002033"
     CDS             complement(13..819)
                     /locus_tag="ACFM4S_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6051640.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILNR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="ACFM4S_002034"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="ACFM4S_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6051641.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFINNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          678 a          625 c          643 g          524 t
ORIGIN      
        1 tgttggctct tcttatctgt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcatcagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 gcgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 catcagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccgaccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt catcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagta gcgggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggcaggtt tgcagggcgc aggcaagacg
     1381 acgaccgtcg gcaaactcgc ccgcctgttg aaaaacgatc agaagaaaaa ggttttggtg
     1441 gtatccgccg acgtttaccg tcctgccgcg attgaacagc tgcgtctgtt ggccgaacag
     1501 gtcggcgtgg attttttccc gtccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgtattga tggtcgatac cgccggccgt
     1621 ttggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccggt gtcgtattga ccaagatgga cggcgattcg
     1801 cgcggcggcg cagctttgtc cgtgcgccac gtaaccggca aaccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgttc aaaaaggtat tgatgaagaa
     1981 gccgccgcca aaatggcgaa aaagctgcac aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat ctcgaaacaa atccccgaag gaacggctga aaaagcgatg
     2161 ggcaaagtag aagccatcat caactcgatg acccctaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacgaccgt acaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaagg gaatgaaagg aatgttcccg
     2401 ggtttgtaag ccgatttgac cgaaaacgcc gtctaaaatt tcagacggcg tttttgtttt
     2461 atattctgat
//
LOCUS       DAXHMX010000202         2467 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXHMX010000202 DAXHMX010000000
VERSION     DAXHMX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.202"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            69..1424
                     /locus_tag="ACFM4S_002035"
     CDS             69..1424
                     /locus_tag="ACFM4S_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6051642.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLIITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMMGGLQANMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1428..2438
                     /locus_tag="ACFM4S_002036"
     CDS             1428..2438
                     /locus_tag="ACFM4S_002036"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6051643.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          457 a          669 c          738 g          603 t
ORIGIN      
        1 ggcgaggcaa cgccgtactg gtttttgtta atctactata cttttcaaat caaaaaagga
       61 tttaccttat gtcggaatat acgcctcaaa cagcaaaaca aggtttgccc gcgctggcaa
      121 aaagcacgat ttggatgctc agtttcggct ttctcggcgt tcagacggcc tttaccctgc
      181 aaagctcgca gatgagccgc atcttccaga cgctcggtgc cgatccgcac agcctcggct
      241 ggttctttat cctgccgccg ctggcgggga tgctggtgca gccgattgtc ggccattact
      301 ccgaccgcac ttggaagccg cgtttgggcg gccgccgtct gccgtatctg ctttatggca
      361 cgctgattgc ggttattgtg atgattttga tgccgaactc gggcagcttc ggtttcggct
      421 atgcgtcgct ggcggctttg tcgttcggcg cgctgatgat tgcgctgttg gacgtgtcgt
      481 caaatatggc gatgcagccg tttaagatga tggtcggcga catggtcaac gaggagcaga
      541 aaggctacgc ctacgggatt caaagtttct tagcaaatac gggcgcggtc gtggcggcga
      601 ttctgccgtt tgtgtttgcg tatatcggtt tggcgaacac cgccgagaaa ggcgttgtgc
      661 cacaaaccgt ggtcgtagca ttctatgtgg gcgcggcatt actgattatt accagtgcgt
      721 tcacaatctt caaagtcaaa gaatacgacc cggaaaccta tgcccgttac cacggcatcg
      781 atgtcgccgc gaatcaggaa aaagccaact ggttcgaact cttaaaaacg gcacctaaag
      841 cgttttggac ggttactctg gtacagtttt tctgctggtt cgccttccaa tatatgtgga
      901 cttactcggc aggcgcgatt gcggaaaacg tctggcacac cactgatgcg tcttccgtag
      961 gttatcagga ggcgggcaac tggtacggcg ttttggcggc ggtgcagtcg gttgcggcgg
     1021 tgatttgttc gtttgtattg gcgaaagtgc cgaataaata ccataaggcg ggttatttcg
     1081 gctgtttggc tttgggcgcg ctcggctttt tctccgtttt cttcatcggc aaccaatacg
     1141 cgctggtgtt gtcttatacc ttaatcggca tcgcttgggc gggcattatc acttatccgc
     1201 tgacgattgt aaccaacgcc ttgtcgggca agcatatggg cacttacttg ggcctgttta
     1261 acggctctat ctgtatgccg caaatcgtcg cttcgctgtt gagtttcgtg cttttcccga
     1321 tgatgggcgg tttgcaggcc aatatgttct tggtaggggg cgtcgtcctg ctgctgggcg
     1381 cgttttccgt gttcctgatt aaagaaacac acggcggggt ttgagcgatg agcgataccc
     1441 ccgctacccg cgatttcggt ctgatcgacg ggcgtgccgt aaccggctat gtgctgtcca
     1501 accggcgtgg tacgcgtgtc tgcgtgctgg acttgggcgg gattgtgcag gaattttccg
     1561 ttttggcaga cggcgtgcgc gaaaacctcg tggtgtcgtt cgatgatgcg gcttcctatg
     1621 cggacaatcc gtttcagatt aacaaacaga tagggcgcgt ggccggacgc atccgcggtg
     1681 cggcgttcga catcaacggc aggacttacc gcgtggaggc caacgaaggc aggaacgcgc
     1741 tgcacggcgg ttcgcacggg ctggccgtta cccgtttcaa cgcggtggcg gcagacggcc
     1801 gttcggtggt gctgcgcagc cgcctgcaac agtcggccga cggttatccc aacgatttgg
     1861 atttggatat ttcctaccgc ttggacgagg acgaccggct taccgttagc tatcgcgcca
     1921 ccgcgctcgg cgacacggtg ttcgacccga cgctgcacat ttactggcgg ctggacgcgg
     1981 gcctgcacga tgcggttctg catattccgc agggcggaca tattccggcc gatgccgaaa
     2041 aactgcccgt ctcaacggtt tcagacgacc tcgaagtatt tgatttcagc cggcccaagc
     2101 cgctggatgc cgccgttgcc gccctgcgcc gcgaaacggg tcgggccggt tttgacgacg
     2161 cttaccgcgt gccgtccgat ataggccgtc ccgccgctgt gttgcaagcc ggacgccgcc
     2221 gtcgtatcag catatacagc gaccgcaatg gcttggtcat ctttaccgcc gccccgcagg
     2281 atttcgcgcg gcacgatgcg ggcgtttacg acgcgctggc gaccgaggcg cagacgctgc
     2341 ccgacagcct gcattggccc gagttcggca atattcgtct gaacaagggt gataccaggg
     2401 aggcgacgat tgcttacggc atcgaatccc tttcttagga gcttcctaac accggttgca
     2461 gacggcc
//
LOCUS       DAXHMX010000203         2457 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXHMX010000203 DAXHMX010000000
VERSION     DAXHMX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.203"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(122..1573)
                     /locus_tag="ACFM4S_002037"
     CDS             complement(122..1573)
                     /locus_tag="ACFM4S_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6051644.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1596..2216)
                     /locus_tag="ACFM4S_002038"
     CDS             complement(1596..2216)
                     /locus_tag="ACFM4S_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6051645.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          771 a          579 c          572 g          535 t
ORIGIN      
        1 ataggaataa gaatttggga caaaatgttt aaaccatttt gtcccgactg ctgtgatgcg
       61 gtttttttga actagcagga cgttacctaa aacctgcgtt gcctgaaacc ctttaagacg
      121 gttaaatccc tctgccgtat ttgtattcta caatcatatt atcgtcggta atgacttccg
      181 ccccagccga aggttccgtc atctgaatca gcatacgaga gacaaccttt tgtgctgcag
      241 catccacggt gctgctgtca aatacgtgcc tgccgctttc cggccaaatc aaccgggaga
      301 gacgttgctt gagcagttct ttattaggga aaactaccgg ggttgccgag ccgactacca
      361 tatgcccata gcggtatgca tagggaatac tgtgtacggc ggtagcaaaa gcatgcgggc
      421 tgtgcgtggt attaaacatt acaataccat ccggggtaag gtggctttgc acctgtttta
      481 aaaattccgc actcaacagg ttggtggaat aggcacgcca gtaccaagtc gtattcatca
      541 aaatcaggtc gaatttttca tcaggatggc gacgcagcca tttcctaccg tcatccaata
      601 caatttcaac acgtttgtcc tgcaaaagcg gggcgatttg cggctcgtcc gcgataaggc
      661 tacggtatgc cggattgatt tccgcaacaa tcatcgactg catttccgga atggcagaca
      721 agacgcgcgc ccacgaacct gtactcaatc caacgacgaa aatgcggcgt atgccagact
      781 tcagggaggg tagcagatag gcacgttcga tgccgttgac actattgaat acatcggtat
      841 tgtatgcgcc gtcgtataca ttcgccccat aaacaacctt atcaccatct ctatggtaaa
      901 ccgcaacaat gccgtgtttg ttttcaatca gcctatccgg acggtcagca atattttgaa
      961 agacagaatc cggcagtagg aacatgagga tgccgaacat tagggaaact gctaccgaca
     1021 ctgcattcag tcggagactt ttttggaaca gtgtacaaaa caaagggaca gcagcagaaa
     1081 tcaaacagat gagcaggtaa atctgttggg tggacaagaa atcaagtatc acaaagccga
     1141 taaggaccgg acccaatgca ctgccggcaa cgttggcgaa ataaacattg gaaacctgtc
     1201 gtccggattt gttgccatcc gtacccacat ggtgtacgag cgggaaaatc aaccctctga
     1261 cgacggcaga cagggtaatg aagataccgg cgtggtggac gaagccggaa aaacccgtca
     1321 acaaccacgc agcacccaaa atcaaaaagt cggcaatacc cgcccacaag aagcactgcc
     1381 cgataaaggg aatatcaaca aagcggctgc ggcaaatccg tttgccaaaa tacgcgccga
     1441 cggcgatacc ggtcagaaaa caggcaagag taaatgaaaa tgcctgaggc acggactgtg
     1501 ctgcgaacga aaacatcctt acccacaaga cttctatacc caagctcaat aagccgctaa
     1561 ggaaagaaag catataaatc aaactagtat tcggtttagt gttcactata tccatccttt
     1621 ctgtaacgca gccatactga agcagcaatc agaaggttaa agcaggctgt cagcgcaatg
     1681 gtttgggaga gggtaaaaaa gacgtagaaa aattcggcgg cggcaagcga tccgagtgcc
     1741 gcacccaaag tgttgaaaaa atataaggta ccgatagact cgccaacatt atgtattttc
     1801 cggttaaaaa aacaggtcag caagggcaag gtcgcgccca tcataaaggt aggaagcagc
     1861 aataagagga aattggcagc agcgatgatg ggcaaatcag cctcaactaa aagatgcccc
     1921 aagccggaaa tcagacccct gcttaccaaa ccgaacagac cgatggatac ttcagcgatg
     1981 caaaacaggg ggatgatact tgaaggaaaa cggtcagcaa tgcgtccacc gaaatacgca
     2041 cctacaccca agccgaccat aaatacagaa ataatgacag taatcgaact caaatcgata
     2101 cctatgtgac tgaatagaag cctctgccag ctgacctggt aaatcagggc gcagaatcca
     2161 gaggcgaaaa acaccagcga caatcctttc agggttttac ttgcggtcga attcatattt
     2221 tctattttca aataggcgga actataaatg aaatgtatca atgtgtaaac atttgtatta
     2281 accgtaggaa atcaccgtat tgctcaaact gcaggccggg aaaataaact cgtaagcccg
     2341 caaacggcgt atcactgttc aaactgctgt gcggcttcac ggtgttataa aaataatggg
     2401 gctgtcctgg acaactaaga taaactcgat tttactaatt gttttaaaat ggaaatt
//
LOCUS       DAXHMX010000204         2423 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXHMX010000204 DAXHMX010000000
VERSION     DAXHMX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.204"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            128..2341
                     /locus_tag="ACFM4S_002039"
     CDS             128..2341
                     /locus_tag="ACFM4S_002039"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6051646.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          633 a          769 c          582 g          439 t
ORIGIN      
        1 aggaaaaatc agaaaagttt tgcattttga aaatgagatt gagcataaaa ttttagtaac
       61 ctatgttatt gcaaaggtct caagctgtta caataaactc gtttttatcg gaacggaaga
      121 ccccatcatg accgccatca gcccgattca agacacgcaa agcgcgactc tgcaagaatt
      181 gcgcgaatgg ttcgacagct actgcgccgc gctgccgaac aacgataaaa aacttgtctt
      241 agccgcccgt tcgctggcgg aagcacatta ccccgccgat gccgccacgc cgtatggcga
      301 gccgctgccc gaccacttcc tcggcgcggc gcaaatggtt catgaactcg acctgctccc
      361 cgatgccgtc gccgccaccc tgcttgccga catcggacgc tacgtccccg actggaacct
      421 attggtttcc gaacgctgca acagtaccgt cgccgagctg gtcaaaggtg tggacgaagt
      481 gcagaaactc acccacttcg cccgggtgga cagcctcgcc acaccggaag aacgcgccca
      541 gcaggcagaa actatgcgga aaatgctgct ggcgatggtt accgacatcc gcgtcgtgtt
      601 aatcaaactg gcgatgcgta cgcgcaccct gcaattttta agcaacgccc ccgacagccc
      661 cgaaaaacgc gccgtcgcca aagaaaccct cgacatcttc gccccgctcg ccaaccgttt
      721 gggcgtgtgg cagctcaaat ggcagctcga agatttgggc ttccgccatc aagagcccga
      781 aaaataccgc gaaattgccc tgcttttgga cgaaaaacgt accgaacgcc tcgaatacat
      841 cgaaaacttc cttaatatcc tgcgtacgga actcaaaaaa tacaatatcc actttgaagt
      901 cgccggccgt ccgaaacaca tctactccat ttacaaaaaa atggtgaaga aaaaactcag
      961 cttcgacggg ttgttcgaca tccgcgccgt gcggattctg gttgataccg tccccgagtg
     1021 ttacaccaca ctgggcattg tccacagcct ctggcagccc attcccggcg agttcgacga
     1081 ctacatcgcc aacccgaaag gcaacggcta taaaagtttg cacaccgtca tcgtcggccc
     1141 ggaagacaaa ggcgtggaag tgcaaatccg caccttcgat atgcaccaat tcaacgaatt
     1201 cggtgtcgcc gcgcactggc gttacaaaga gggcggcaaa ggcgattccg cctacgaaca
     1261 aaaaatcgcc tggttacgcc aacttttgga ctggcgcgaa aacatggcgg aaagcggcaa
     1321 ggaagacctc gccgccgcct tcaaaaccga gcttttcaac gacacgattt atgttttgac
     1381 cccgcacggc aaagtcctct ccctgcccac aggcgcgacc cccatcgact tcgcctacgc
     1441 cctgcacagc agcatcggcg accgttgccg cggtgcgaaa gtcgaagggc agattgtgcc
     1501 gctgtccacc ccgctcgaaa acggacagcg tgtcgaaatc attaccgcca aagaagggca
     1561 tccttccgtc aactggcttt acgaaggctg ggtcaaatcc aacaaggcaa tcggcaaaat
     1621 ccgcgcctac atccgccagc aaaacgccga caccgtgcgc gaagaaggcc gcgtccaact
     1681 cgacaaacag cttgccaaac tcacgcccaa acccaacctg caagagcttg ccgaaaatct
     1741 cggctacaaa aagccagaag acctctacac cgccgtcgga caaggcgaaa tttccaaccg
     1801 cgccatccaa aaagcctgcg gcacgctgaa cgaaccgccg cccgtacccg tcagcgaaac
     1861 caccatcgtc aaacagtcca aaatcaaaaa aggcggcaaa aacggcgtgc tcatcgacgg
     1921 cgaagacggt ctgatgacca cgcttgccaa atgctgcaaa cccgcgccgc ccgacgacat
     1981 tgtcggcttc gttacccgcg atcgcggcat ttcggtacac cgcaaaacct gcccctcttt
     2041 ccgacacctc gccgaacacg cgcccgaaaa agtactggac gcaagttggg cggcgttgca
     2101 ggaaggacaa gtgtttgccg tcgatatcga aatccgcgcc caagaccgct ccgggctttt
     2161 gcgcgacgta tccgacgcgc tcgcccgcca caaactcaac gttaccgccg tgcaaaccca
     2221 gtcccgcgac ttggaagcca gtatgcggtt cacgctcgaa gtcaaacagg tcaacgacct
     2281 cccgcgcgtc ctcgccagcc tcggcgacgt caaaggcgta ttgagcgtta cccggcttta
     2341 aatacaaaaa tgccgtctga aagccgaata acgcttcaga cggcattttg attgccgggg
     2401 tttgctattt tttgttgcat agt
//
LOCUS       DAXHMX010000205         2345 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXHMX010000205 DAXHMX010000000
VERSION     DAXHMX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.205"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..160)
                     /locus_tag="ACFM4S_002040"
     CDS             complement(<1..160)
                     /locus_tag="ACFM4S_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6051647.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGN"
     gene            complement(200..325)
                     /locus_tag="ACFM4S_002041"
                     /pseudo
     CDS             complement(200..325)
                     /locus_tag="ACFM4S_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(654..1064)
                     /locus_tag="ACFM4S_002042"
     CDS             complement(654..1064)
                     /locus_tag="ACFM4S_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051648.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(1652..2035)
                     /locus_tag="ACFM4S_002043"
     CDS             complement(1652..2035)
                     /locus_tag="ACFM4S_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051649.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(2001..>2345)
                     /locus_tag="ACFM4S_002044"
     CDS             complement(2001..>2345)
                     /locus_tag="ACFM4S_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC6051650.1"
                     /translation="NKPPKPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGTIYVDSKG
                     NAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHHANTHQDPRALTGHAHVPGV
                     TNDDGTPWLPIK"
BASE COUNT          679 a          424 c          401 g          841 t
ORIGIN      
        1 cattaccaaa cagatggtat ttaccacccg gttcgtagtg ttttgcctgc atatcatcgc
       61 gtaaacgggc atccaaaccg ttcgcatgac taatggggat ttgcagcaac gatattgccg
      121 ctgcaaacag catcatgaat ttttgaatag gcaatttcat tttgttttcc ttggtagcaa
      181 gcggttatgg tcgtgtgaat caaggttgcc cttgtctatg ttgtcgcact acttcatcgc
      241 tgtatccata cccctcatga ctgttatcag cctctacgga tggggcagag ttacccgtat
      301 gattgccgta tggtcggata cttgtatccg acaaaaatat ttgacgcgtc tatcgtttcc
      361 gaaatgccgc tgttgaaaaa tgtcggattc gagaatccga cctacggttg ctgaacaatg
      421 ttttgtagaa cttgctcaaa atccatgttt cgccattttc ctttatatat acctaataca
      481 gcacgggtta cgatttcgtt taagtcataa atagccattt acgtcctctg ctaattttca
      541 gcgagtagat tgggttacca aacccaacaa aaaagcagcc tgaaaaattt caggatgctt
      601 gttttatgtt ttttatcaat gaattaggtt ttaatccagc ctgcgcttgc tagttaaaca
      661 gtatttacat aaaaatcttg tactgaacaa acaagcgtat catgttcatc atgaaatgat
      721 gggcataacg ttaatctaca aatgtttaca tttaaagata ttttttcagt ttcaatattt
      781 ttaatttgta aggttaatac atgaggatat ttgtgatata gctgattttt taattgttcg
      841 ttgtctaata tagcaaaagg taattttgca cacaaataac caataacttt gtttttatga
      901 aaaaaaacta tccaattttc aattccttca tcccaatcaa aagtaaaatc cgttccttct
      961 atgataaatt tagcacaaga tattaaatct aacttaaccc aagtggattt atacagactc
     1021 aatgctgatg aagttataat attatctaaa ttataaattt tcataaatca acgttcctta
     1081 cctggtgtta tatgcacagg tctaatccca tctttattcg gctttctacg ataaatgtta
     1141 attatatgtg tcggttttcc gtctgtatag ccaactacgt cgttcgttgg aacttttgcc
     1201 ttattttgac tatcccataa attatgttta tctgcatatc tagcagcatc taatgtagct
     1261 ttttccgcat caacatctga tttaaataaa cttttgctat ctatcttgtc tagactagat
     1321 gcatcaggtt tgtgtcgtaa aaagcactgt cctgtaatag gctcgcggca gcaagttgtg
     1381 cttccggact taattttcct aaatcattaa taccttgtaa agtttgctgc gcagagatgt
     1441 aggtcggata tttgtatccg acaaaaatat ttgacgcatc tatcgtttcc gaaatgccgc
     1501 tgttggaaaa tgtcggattc gagaatctga cctacggctg ctgactattt tcttgtttat
     1561 ataattctac aactgcaaac tactatcaaa tctttttatc tctggctcta cactcagcaa
     1621 gaatattttt attttacaaa acataggcta gttatttgag tatattgtca aattctaatt
     1681 tgtttttttg atagattttt tttataccat taatacattc attagttgta attctaccaa
     1741 gcgaatgtaa tttatcaata tcaaaacaat cggtataaac cctattcaca gttttatctt
     1801 gtatttcctt atgaatactt aatatattga gatagtttag aaattttatt ttctcagatt
     1861 gaggtaactc atcaaaacca agccaataat aaaaattgtt atatgatttt aaaaaatcaa
     1921 caatttttat ttttcctaaa accaaatctt caactaactg ggttgcataa ataatatcat
     1981 ttatgatttt aacaggtaaa ttatttgatg ggtaaccatg gggttccgtc atcatttgta
     2041 actcctggta catgtgcgtg tcctgtaagt gcgcgtgggt cttgatgggt atttgcatga
     2101 tgagcatcat ctattctggc tccagtcaag tttccattac tatctcggac ttggataaaa
     2161 cgagcatcag gcgaacctgt tatataaccg cctattggtg ttggtattgc atttcctttg
     2221 ctgtctacat aaattgttcc tgtcggagaa atagatgtat caggtttaac atatggggca
     2281 ggtaagccta tattgttatc agagtgtgta gagtgttgct gcgtacttgg tttcggtggc
     2341 ttatt
//
LOCUS       DAXHMX010000206         2326 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXHMX010000206 DAXHMX010000000
VERSION     DAXHMX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.206"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            299..733
                     /locus_tag="ACFM4S_002045"
     CDS             299..733
                     /locus_tag="ACFM4S_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051651.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            1009..1728
                     /locus_tag="ACFM4S_002046"
     CDS             1009..1728
                     /locus_tag="ACFM4S_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051652.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            1715..>2326
                     /locus_tag="ACFM4S_002047"
     CDS             1715..>2326
                     /locus_tag="ACFM4S_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051653.1"
                     /translation="MSWNSFGNFMSARSSALAEFGNMVANLVSAKNEKDISKRNEYYK
                     QAGYSALLAFGNLASNIAPGSTSSHIVNGTNASVIASRLSGNISSAIQEHKDGKVNIN
                     RFQNILADLYSLGGLGSTLIEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEF
                     YNFDNWKGFGYELFEDWSRWVYDWLPDGWNLWKELDRNRSGQYH"
BASE COUNT          798 a          284 c          420 g          824 t
ORIGIN      
        1 caaacaaaaa agagaaattt taatttcgct aaatcgcata aagattaatc aagagtatca
       61 ttaaatgata tgagtgagca tctataatgc caagaagagt tgttaagaca taacgattat
      121 tgaaatagat tgtaaaatag atacttagat agtctgaaaa acggatttgt gaaacttttt
      181 attacgcgcc atcatttgaa aatgaaactt aaaaaacact tatcataata aatattttct
      241 ttacgttgtt tgctaataaa ctcagtgcaa tatcagcgca atattttatg gaaattttat
      301 ggataacaaa aaagaattta ttaataattt aacaaatagg tatatgtgga tctatccatt
      361 ggtcttaaat attctatttc taccttttta ccagtcctac caatcttttt ttattgcgct
      421 tggttgtttg tttgcactgg ttagaaaaat gcaaagctta gattttaaat tacaaaatca
      481 tattgtattg ttaaatataa aaagtgcttg ggcagataaa aaagtatttt tgattaggat
      541 agtagtgtca tggttggcag taatggaaat atggatgtgt tttatttcgg aatcatcaac
      601 gtgggtatgc ggtgcttttt gtttaaatag tgaaatattg gaaaaaattt ttcgtggctt
      661 tggttattct ggtagtttat attttttatt tatattgatg attgatctca ataagttaag
      721 agagagtatt tgaatttcat tttttgtttg acttaaactc aaggagagta acaatgattg
      781 gtagtggtga tactaaacaa tgcaaaaaat tttctgcgtg tgatggaaaa taccacgtct
      841 acgatcccct cgccctagac ttggacggcg acggcataga aacagtcacc gccaaaggct
      901 tttcaggcag cctgaagact gagagagtga atacgatgag tatacactct atgccactaa
      961 attgatattc actaaatcat accagctata ttttatttaa tgagacatat gaaaaataaa
     1021 aattatttac tagtatttat agttttacat atagccttga tagtaattaa tatagtgttt
     1081 ggttattttg tttttctatt tgattttttt gcgtttttgt tttttgcaaa cgtctttctt
     1141 gctgtaaatt tattattttt agaaaaaaac ataaaaaaca aattattgtt tttattgccg
     1201 atttctatta ttatatggat ggtaattcat attagtatga taaatataaa attttataaa
     1261 tttgagcatc aaataaagga acaaaatata tcctcgatta ctggggtgat aaaaccacat
     1321 gatagttata attatgttta tgactcaaat ggatatgcta aattaaaaga taatcataga
     1381 tatggtaggg taattagaga aacaccttat attgatgtag ttgcatctga tgttaaaaat
     1441 aaatccataa gattaagctt ggtttgtggt attcattcat atgctccatg tgccaatttt
     1501 ataaaatttg caaaaaaacc tgttaaaatt tatttttata atcaacctca aggagatttt
     1561 atagataatg taatatttga aattaatgat ggaaacaaaa gtttgtactt gttagataag
     1621 tataaaacat tttttcttat tgaaaacagt gtttgtatcg tattaattat tttatattta
     1681 aaatttaatt tgcttttata taggacttac ttcaatgagt tggaatagtt ttggtaattt
     1741 tatgagcgca cgctcatccg cgttagcaga atttggaaat atggttgcta atttagtttc
     1801 tgcaaaaaat gagaaagata tctcgaaacg taatgaatat tacaaacaag ctggttatag
     1861 tgcattatta gcatttggta atttggctag taatattgca ccaggtagta cgtcatcgca
     1921 tattgtaaac ggaacaaatg cctctgtgat tgcaagccgt ctctctggaa atatatcttc
     1981 agctattcag gagcataaag atggtaaagt taatatcaac cgttttcaaa atattttagc
     2041 ggatttatat tcattgggag ggttaggaag tacattaata gagaagaatg gaaatatgca
     2101 gagttggggg attccattag caattgctgg agatataatt gcagcaacgg ctattgccac
     2161 aggagatact ggtacgatat ctacagagga attttataat tttgacaact ggaaaggttt
     2221 tgggtatgag ctatttgaag actggtctcg ttgggtatac gactggctgc ccgacggctg
     2281 gaatctgtgg aaagaattgg acagaaaccg ttcaggccaa taccac
//
LOCUS       DAXHMX010000207         2309 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXHMX010000207 DAXHMX010000000
VERSION     DAXHMX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.207"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..567)
                     /locus_tag="ACFM4S_002048"
     CDS             complement(<1..567)
                     /locus_tag="ACFM4S_002048"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6051654.1"
                     /translation="MEHKLPQLPYEPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAK"
     gene            731..2137
                     /gene="dnaB"
                     /locus_tag="ACFM4S_002049"
     CDS             731..2137
                     /gene="dnaB"
                     /locus_tag="ACFM4S_002049"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6051655.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          555 a          599 c          624 g          531 t
ORIGIN      
        1 tttggcgact tcgtcccagt tgacgatttc ccagaaacct ttcaggtagt tggggcggct
       61 gttgcggtag tcgatgtagt aggcgtgttc ccacacgtcg caggtcagca gcggcgtgtt
      121 ttcagtggtc agcggcgtag cggcgttgga agtggaaacc aaatccaatc cgccggcagg
      181 ggttttcacc agccatgccc aaccggagcc gaaagtaccg gccgcgcagg cattgaacgc
      241 ttcttggaat ttctcgaagc tgccccattt cgcgtcgatg gcggcggcca gttcgccgga
      301 aggtttgcct tgacctttgg gcgtgaaacc cagccagtag aaggtgtggt tccaagtttg
      361 tgccgcgttg ttgaacacgc cgcccgaaga ttttttcaca atctcttcca aaggcaggtt
      421 ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt gatggtgttt
      481 gccgtagtgg aattccaaag tctctttgct cagatgcggg gacaatgcgt ccggttcata
      541 aggcagttgc ggcagcttat gttccatttt gtgctcctaa tgttgttttt ggatgttgtg
      601 ttttggcagt gttgctgcaa acgaatcggc agcacacgca ttctacctgt tttgagggat
      661 ggaaaccaat tgaacctgct ttacgctata atagaagatt gcaatttcgg cacgacagat
      721 aggatgtacc atgaacgatt acgccgctat gccgtctgaa gaccgtgagg tcggcgcatt
      781 gtcgctgcct ccacattcga tggaggcgga acaatccgtt ttgggcggtt tgatgctgga
      841 aaatccggct tgggacagga ttgccgatgt ggtttccggt gaagacttct accgccacga
      901 acaccgcctg attttccgat ccattgctaa attaatcaat gagagccgtc ccgccgatgt
      961 gattacggtt caggaagatt tgcagcggaa cgaagaattg gaagcggcag gcggattcga
     1021 atatctgatt acgctggcgc aaaacacccc gtctgccgcc aacatccgcc gctacgccga
     1081 aatcgtgcgc gagcgttcca tcatgcgcca actcgccgaa gtggggacgg aaatcgcccg
     1141 cagcgcatac aatccgcaag gcagggacgc ggggcagctt ttggacgagg cggaaaacaa
     1201 agtattccaa atcgccgaaa gcaccgccaa atccaagcag ggctttttgg agatgcccga
     1261 tttgctgaaa gaagtcgtac agcgcatcga tatgctctac tcgcgcgaca atcccgatga
     1321 agttaccggc gtgccgacgg ggttcatcga cctcgacaaa aaaacctcgg gccttcagcc
     1381 cggcgacctg attattgttg ccggtcgtcc gtctatgggt aagaccgcct tttctatcaa
     1441 tatcgccgaa cacgttgccg tagaaggcaa gctgccggtt gccgtcttct caatggagat
     1501 gggcggggcg caactggtca tgcgtatgct cggctcggtc ggacggctgg atcaaagcgt
     1561 tttgaaaacc ggcaggctcg aagacgaaca ctggggtcgc ctgaacgaag cagtcgtcaa
     1621 actctccgac gcgcccgtgt acatcgacga gaccccgggt ctgaccgcgc tcgaactgcg
     1681 cgcccgtgcc cgccgtctcg cccgtcaatt taacaataag ctgggattaa tcgtcatcga
     1741 ctacctgcaa ctgatggcag gatccggccg ttccgacaac cgagcttcag agctgggcga
     1801 aatttcacgt tcgctcaaag cgttggcgaa agaattgcaa gtccccatca tcgccctgtc
     1861 gcaattgagc cgtaccgtcg aatcgcgtac cgacaaacgc ccgatgatgt ccgacctccg
     1921 cgaatccggt gctatcgagc aagatgccga cctgattatg ttcatgtacc gcgacgaata
     1981 ctacaaccag gactcaccca tgaaaggcct tgccgaatgt atcatcggca aacaccgcaa
     2041 cggtcccgtc ggtaaaatct tcctcacatg gacggggcaa ttcaccaaat tcgacaatgc
     2101 tgcctatatt cccgaggagg caaagataga ggattaaatg gctatataaa aatttattag
     2161 gcgaaatcag gcaaaatcgt ttaaaatcat gcttagagat tgccctaaaa aataaaacgc
     2221 ggtcttgagg catttttgca ttcagcccgc atataattga aaatatagtg gattaacaaa
     2281 aatcaggaca aggcaacgaa gccgcagac
//
LOCUS       DAXHMX010000208         2287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXHMX010000208 DAXHMX010000000
VERSION     DAXHMX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.208"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            20..748
                     /gene="rpe"
                     /locus_tag="ACFM4S_002050"
     CDS             20..748
                     /gene="rpe"
                     /locus_tag="ACFM4S_002050"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6051656.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="ACFM4S_002051"
     CDS             complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="ACFM4S_002051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6051657.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1884..2192)
                     /locus_tag="ACFM4S_002052"
     CDS             complement(1884..2192)
                     /locus_tag="ACFM4S_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051658.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          463 a          549 c          733 g          542 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcggcccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgttga
      241 cgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat atcgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtca accccggatt cggcggacaa agcttcatcc cgcacaccct
      481 tgaaaaaatc cgccgggtgc gggcgatgct ggatcggtac gaagcacaaa gcgggcggca
      541 catcgccatc gaagtggacg gcggcatcaa aaccgacaat attgccgccg ttgcccgagc
      601 tggtgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatagaa atgccgtctg aaaattagct ctgtttcaga cggcattgcg ttatttttcg
      781 acgggcagcc cgaaatgcag gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt
      841 tgcaggaagc cttgttggat aaggtagggt tcgataacgt cttcgatggt gtctgttgat
      901 tcgccgatgg cggcggcaac attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa
      961 acggcttcga ggaatttcct gtccatcacg tccagcccct gcacgtccac gtccagcata
     1021 cttaaagcgg catcggcgat gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa
     1081 tcgcgcacgc ggcgcaacag gcggttggcg atgcgcggcg taccgcggct gcgtttggcg
     1141 atttcttccg cgccttcttc ggacatatcg agttgcagga gttgtgccga acggctgacg
     1201 atggtggcaa ggtcgcggtt ttcgtaaaac tcgaggcggg agacgatgcc gaagcggtcg
     1261 cgcaacggat tggtcagcat accggcgcgg gtggtcgcgc cgacaagcgt gaagggcggc
     1321 aggtcgattt taacggaacg ggcggcgggc ccttcgccta tcataatgtc gagccggtag
     1381 tcttcaagcg cggggtagag gatttcttcg acaacagggc tgaggcggtg gatttcgtcg
     1441 ataaacaata cgtcgtgcgg atcaaggttg gtcaaaaggg cggcgagatc gccggcgcgt
     1501 tcgaggacgg ggccgctggt ttggcgcaaa tttacgccca attctttggc gatgatgtgc
     1561 gccagtgtgg ttttacccag tccgggcggg ccgaagagca aaacgtggtc gagtgcttcg
     1621 ccgcgttttt tggcggcttg gatgaaaatg gcgagctgtt ctttggcttt atgctgcccg
     1681 atatagtcgt ccagcgtttt ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag
     1741 gcggtttggg cggcaacaat gcgttgcggt tgagcggcag tcaggttgtc ggtttgcagc
     1801 atagtgttcc ctttgtcggg tatgccgtct gaacggtcgg cggcgtttca gacggcattg
     1861 aaaagataac ggtcaaaagc gttttacccg cgcgtttcgc gccaaacgag atagtcgcgc
     1921 aggggcggca taggcgggtt gatgcagtgt gggcagatgc ctgtggtgtc ttcgtcgtcg
     1981 ctgtcgatgt gataggcgtt ggggtcgaag cggacttgct gcatcaccgc ctgcgccaac
     2041 gcctgcgcct gaacaacgtc gaaattgaat ttttcgagga tttcgttcaa taggcggatt
     2101 tcgccaccgc taaattccgc actgtgtttg aggatgaggc gttgataatc ttcgttgttg
     2161 attttaggca ataaatcggt tttcagggtc atgattttat gtcgggggcg gaatggctga
     2221 agcgtgaatt atagcggata tggcggcggc tgttgcaaag cttggattgg gtatcgtcct
     2281 caaaact
//
LOCUS       DAXHMX010000209         2283 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXHMX010000209 DAXHMX010000000
VERSION     DAXHMX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.209"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            295..807
                     /locus_tag="ACFM4S_002053"
     CDS             295..807
                     /locus_tag="ACFM4S_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6051659.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            887..1966
                     /gene="aroB"
                     /locus_tag="ACFM4S_002054"
     CDS             887..1966
                     /gene="aroB"
                     /locus_tag="ACFM4S_002054"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6051660.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          562 a          709 c          548 g          464 t
ORIGIN      
        1 ttgataaaca tattatcttc aatatattca attggataat tgtttaccta agcaaagata
       61 attgcctttt cctgacaaat aagtgaaatc aacggattgt caaaacacag cctgaaataa
      121 aaaacctccc tgatttcttt tatttgtcct taaaatcaga aaggttcggg atggtcgggt
      181 tatttttcca aacgtaccgc cgccctgccg atttcgtata aaattccgcc gtaacccgac
      241 aagcccgaac cctgtcgccc cgaaaggcgg ggtgtcaaac attaaggaat tgtgatgaaa
      301 aactttaacg gcaaactcat cctcatcgga ctgatgggcg cgggcaaaac cacgctgggc
      361 cggcaaatgg cgcagcggct ggattaccgt ttttacgaca gcgatcacga aatcgccgca
      421 gccgccggcg ttcccatccc caccatattt gaaatggaag gcgaacaggg attccgttcg
      481 cgcgaaaccg ccatactcaa aaagctggtt atcctgcccc atatcgtcct gtccaccggc
      541 ggcggcgcgg tgttaaaaga agaaaaccgc gcccttatcc gcaaaagcgg cacggtcgtc
      601 tatctgcacg ccccgcccga aaccctgctc gaacgcacgc gctgcgacaa cagccgtcct
      661 ttgctgcaag ttgccgatcc tttggcgaaa ttacgtgaac tctacgccgc acgcgacccc
      721 gtttaccgcc aaaccgccga ctttaccgta gaatcggcaa actgccggga aaccgtgcaa
      781 accctgctca aacgcttatc ccgataaacc ggcatatgcg ccgcgcccag aaaaccaagc
      841 cgcgccccgc ccggcgggcc ggcggttcaa actttaagga acaacaatga aaacactgac
      901 cgtacacacc ccgtcccaca gctaccccat ctttatcggc aacggactgc tgccgcaggc
      961 aggaagcctg ctcaaaccgc atttgggcaa acgcgccgcc atcatcgcca acgaaaccgt
     1021 cgccccgctc tacctcggca cgcttcagac ggcattggat gcggcaggcg tatcccattt
     1081 cagcatcatc ctgcccgacg gcgaggcgca caaaaactgg cagacgctca acctcatctt
     1141 tgacgggctg atgcaaaacc gcgccgaacg caaaaccaca ttaatcgcac tgggcggcgg
     1201 cgtgatcggc gacatggtcg gctttgccgc tgccacctac cagcgcggcg caccgttcgt
     1261 tcaaataccg accacactgt tgagtcaagt cgactcatcg gtgggcggca aaaccgccat
     1321 caaccacccg ctcggcaaaa atatgatcgg cgcattctac cagccgcagg cggtgcttgc
     1381 cgacttggac acgctgcaca ccctgcccgt ccgcgaattg tccgccggta tggcggaagt
     1441 catcaaatac ggcgcgctcg gcgacatcgg ttttttcgaa tggctcgaac aaaacatatc
     1501 cggactgatg acgctcgatc gggaaaaact cgcccaagcc gtgtaccgct gctgccaaat
     1561 gaaggcagac atcgtcgccc aagacgaaac cgaacagggc atacgcgcat ggctcaacct
     1621 cggacacacc ttcggacacg ccattgaaac cgagatgggt tacggcactt ggctgcatgg
     1681 agaagccatc gccgccggct gcgtgttggc ggcgcgtttg tccgaacaac tgggcaaaac
     1741 ctccgccgca gataccgcgc ggctcgccgc cctgctcgaa gccgctggac tgccgtccgc
     1801 accgcccgtg tttgcctttg aaaaatggct cgaacatatg agccacgata aaaaagtcag
     1861 cggcggcatc atgcgcttta tcggtctgaa ccggctgggc gaagccaaca tcaccgaaat
     1921 taccgacacg gacatcctcc gccgcaccct gcaaccgtat ctctgatttc ctctgccgat
     1981 gtgctgccgc gcgggtttga cgcacgatga tgattttcca tcatctttct ccgtaaaagc
     2041 gggaatccgg tccgttcggt ttcggtcgtt tccgataagt tcccgttgct tttcatttct
     2101 agattcccac tttcgcagga atgacggtgg aaaggttttt gttgtttcgg agaagtttct
     2161 gcaaccctag aatctcgtta tttccacaaa aaacagaaac ccaaaacagc aacttaaaac
     2221 ctcgtcattc ccgcaaaagc gggaatccgg tccgttcggt ttcggtcgtt tccgataaat
     2281 tcc
//
LOCUS       DAXHMX010000210         2261 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXHMX010000210 DAXHMX010000000
VERSION     DAXHMX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.210"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            144..1955
                     /gene="typA"
                     /locus_tag="ACFM4S_002055"
     CDS             144..1955
                     /gene="typA"
                     /locus_tag="ACFM4S_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6051661.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          574 a          638 c          590 g          459 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tataccgtct gaaaggcaag ggcttcagac
       61 ggcatcttca tttcccaaat actgtccggt aaagcgtggt ataatgcgca gttatctctt
      121 acagtttttg aaaaacatta attatgaaac aaatccgcaa catcgccatc atcgcccacg
      181 tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg ccaatccggc acattccgag
      241 ccaaccagca ggttgacgag cgcgtgatgg acagcaacga cctcgaaaaa gaacgcggca
      301 tcaccatcct cgccaaaaac accgccatcg attacgaagg ctaccacatc aatatcgtcg
      361 atacgccggg acacgccgac ttcggcggcg aagtagagcg cgttttgggg atggtggact
      421 gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc gcaaacccgt ttcgtgacca
      481 aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat caacaaaatc gacaagccgt
      541 ccgctcgtcc gagctgggtc atcgaccaaa ctttcgagct gttcgacaac ttgggcgcga
      601 ccgacgagca gttggatttc ccgattgttt acgcttcagg gttgagcggt ttcgccaaat
      661 tggaagaaac cgacgagagc aacgacatgc gtccgctgtt cgatactatc ttaaaatata
      721 cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact gcaaatttcc caactcgact
      781 acgacaacta caccggccgc ctcggtatcg gtcgtatctt gaacggacgc atcaaacccg
      841 gccaaaccgt tgccgtcatg aaccacgatc agcaaatcgc ccaaggccgc atcaaccagc
      901 ttttgggttt caaaggtttg gaacgcgtgc cgcttgaaga agccgaagcc ggcgacatcg
      961 tgattatttc cggtatcgaa gacatcggca tcggcgtaac catcactgat aaagataacc
     1021 ccaaaggcct gccgatgttg agcgtggacg aaccgacgct gaccatggac tttatggtta
     1081 acaccagccc gttggcgggc acggaaggca aattcgtaac cagccgccaa atccgcgacc
     1141 gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt ggaagatacc gccgatgccg
     1201 acgtgttccg cgtatccggg cgcggcgagc tgcacctgac cattttgctg gaaaacatgc
     1261 gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt cgtgtaccgc gacatcgacg
     1321 gtcaaaaatg cgaaccgtat gaaaacctga ccgtggacgt acccgacgac aaccaaggcg
     1381 cggtaatgga agaactcggc cgccgccgtg gcgaactgac taatatggaa agcgacggca
     1441 acggacgcac ccgcctcgaa taccatattc cagcgcgcgg cttgatcggc ttccaaggcg
     1501 aatttatgac cctgacgcgc ggggtcgggc tgatgagcca cgtgttcgac gattacgcgc
     1561 ccgtcaaacc cgatatgcct ggccgccaca acggcgtgct ggtgtcccaa gagcagggcg
     1621 aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg ccgtatgttc gtatcgccca
     1681 acgacaaaat ctacgaaggt atgattatcg gcatccacag tcgcgacaac gatttggtgg
     1741 tcaacccgct caaaggcaaa aaacttacca acatccgtgc cagcggtacc gacgaagccg
     1801 ttcgcctgac cacgccgatt aagctgacgc tggaaggtgc ggtcgagttt atcgacgatg
     1861 atgagctggt agaaatcacg ccgcaatcca tccgtctgcg caagcgttac ttgagcgaat
     1921 tggaacgccg ccgccatttc aaaaagctag attgatgttt actggattaa tgtttaaatg
     1981 atacgcgatg ccgtctgaaa aatttcagac ggcatttttt attcggacgg gctttgcggc
     2041 ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc aagaaactgc cgccattttt
     2101 ccagcggcat atcgccccgt cgcgtttcgg tatcgggttc ggcttcccgg cagcatgtcg
     2161 aacaatgccg tttgaaggaa tacgcctttt gagtccttac aagcttagga agaggatata
     2221 gtggattaac aaaaatcagg acaaggcgac gaagccgcag a
//
LOCUS       DAXHMX010000211         2252 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXHMX010000211 DAXHMX010000000
VERSION     DAXHMX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.211"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            569..1384
                     /locus_tag="ACFM4S_002056"
     CDS             569..1384
                     /locus_tag="ACFM4S_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6051662.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHKLYYIKKGENPAIITHRNNRINQTEED
                     NYSTSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            1401..>2252
                     /locus_tag="ACFM4S_002057"
     CDS             1401..>2252
                     /locus_tag="ACFM4S_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6051663.1"
                     /translation="MNEGEVVLTPEQIQTLYGYASRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTYHGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSESDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYH"
BASE COUNT          762 a          351 c          469 g          670 t
ORIGIN      
        1 caaaaatata ccaaaaacag caaaacaaat tgtaaggata cgtataggct ttgtaaaggt
       61 aaattgtgaa aaaagcagtt ttttaaacga atgaaacggc ttcgggctga aatatatgct
      121 gatgccctgt tcttcccgta tttctcgtgt gttgtcaaag tgcaggctgc tttgaaatcg
      181 gtattgccat ctatgaacca ccactttgct ttatttcagc gggcttgaga tgtgtataag
      241 gatattgttt tgaataaatt taaagaaaat gataatcgtt attgacgatt tttaaaggaa
      301 agcgtagagt gccaattcta tgaagcaata cggtaagtaa caatgaaaat atctactgct
      361 tgggtataga gcatatttca caacccgtaa ctattcttgc ggaaacagag aaaaaagttt
      421 ctcttctatc ttggataaat atatttaccc tcagtttagt taagtattgg aatttatacc
      481 taagtagtaa aagttagtaa attattttta actaaagagt tagtatctac cataatatat
      541 tctttaacta atttctaggc ttgaaattat gagaccatat gctactacca tttatcaact
      601 ttttattttg tttattggga gtgtttttac tatgacctca tgtgaacctg tgaatgaaaa
      661 gacagatcaa aaagcagtaa gtgcgcaaca ggctaaagaa caaaccagtt tcaacaatcc
      721 cgagccaatg acaggatttg aacatacggt tacatttgat tttcagggca ccaaaatggt
      781 tatcccctat ggctatcttg cacggtatac gcaagacaat gccacaaaat ggctttctga
      841 cacgcccggg caggatgctt actccattaa tttgatagag attagcgtct attacaaaaa
      901 aaccgaccaa ggctgggttc ttgagccata caaccagcaa aacaaagcac actttatcca
      961 atttctacgc gatggtttgg atagcgtgga cgatattgtt atccgaaaag atgcgtgtag
     1021 tttaagtacg actatgggag aaagattgct tacttacggg gttaaaaaaa tgccatctgc
     1081 ctatcctgaa tatgaagctt atgaagataa aagacatatt cctgaaaatc catattttca
     1141 taaactttac tatattaaaa aaggagaaaa tccggcgatt attactcatc ggaataatcg
     1201 aataaaccaa actgaagaag ataattatag cactagcgta ggttcctgta ttaacggttt
     1261 cacggtacgg tattacccgt ttattcggga aaagcagcag ctcacacagc aggagttggt
     1321 aggttatcac caacaagtag agcaattggt acagagtttt gtaaacaatc caagtaaaaa
     1381 ataatttaaa ggatcttatt atgaatgagg gtgaagttgt tttaacacca gaacaaatcc
     1441 aaaccttgta tggttatgct tctcgtggcg atacctatgg cggttggcgt tatttggcta
     1501 atttgggtga ccgttatgcg gataatgctg ccgcaattgt cggtaaggat gcaaacttaa
     1561 atggtttgaa tttatggatg aaaaaagggg tggaaaacct atgggatgat acggtcggta
     1621 aaaagacccg tttagagaaa tttgatcggg ttgcattgca acatttcagc caatatgtag
     1681 atctaattaa tgaaaataat ggtagattac ctaacactag tgaaattgag agaagttact
     1741 ataaagccgt tacctatcat ggcgtttctt ctagtgcagc tattgattta gttattaatc
     1801 gttcacttcc ggatatggcg gatggttatt gggcattagg tttggggata gaagccgaac
     1861 gtatccacaa tgagcaagca gtaaataatc cgaacggtag cgaatcggat aatagaaagc
     1921 agttaatatc tgctttagat aaaggatttg atggatcttt taaagagaag cattttactt
     1981 ttttacaatc tgtgataatg gatgtaacaa agttaggtgt tgaatataca atagatggtt
     2041 ggcaaaaaat tggaggttgg ggtaatggga taatcaatga tttatataaa agtgttgcaa
     2101 aaagagagtg gactggaata tttgagatcg ttaataataa catcaagcaa tttagagatc
     2161 tgttcccaaa tccggaaggc tggatcgatg atggtcacca atgtttcgct ccttgggtta
     2221 aagaaactaa aaaacgcaat ggcaaatatc at
//
LOCUS       DAXHMX010000212         2216 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXHMX010000212 DAXHMX010000000
VERSION     DAXHMX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.212"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            136..2016
                     /locus_tag="ACFM4S_002058"
     CDS             136..2016
                     /locus_tag="ACFM4S_002058"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HFC6051664.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELGKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVKPSDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDSK
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPNRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVALAGKTAGN
                     AV"
     gene            2006..>2216
                     /locus_tag="ACFM4S_002059"
     CDS             2006..>2216
                     /locus_tag="ACFM4S_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilS"
                     /protein_id="HFC6051665.1"
                     /translation="MPSEARYGKLRQKQKPESHHSHSRESGFLGFRCFSVSMNFQAAV
                     PDKYPQSKIPSFPRRWESRFIRTETD"
BASE COUNT          601 a          674 c          518 g          423 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctctcc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgatgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcac aggcggcgga catcgacacc gatttattgt gggaagtatg cggcgaagag
      361 gaatttaccg ccgaagccat cgccgaagaa tattacggcc atgcgccgac caaaaccgag
      421 ctggcggcaa ctttgattgc gctttacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaacc ttaaaacaag cgcttgccgc catcgaacgc
      541 aaaaaacagc aggacgcaca aatcgacgct tgggcagaag ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaactgggg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tacttcccca aaggaacagg ctttcccgac cttccccttc ccgaaatgcc cgacctgccc
      841 aaagccgatg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgatgcc
      901 ttgagcctga ccgacttggg caacggcacg aagcgcgtcg gtatccacat cgccgcgccg
      961 tcgcttgccg ttaaaccgag cgacaaaatg gaaaaaaaca tcatggaacg cttgagtacg
     1021 gtttatttcc ccggcggcaa aatcacgatg ctgcccgaaa actggattgc cgcgttcagc
     1081 cttgatgcag gcgcacaccg ccctgccgtc agcatttatt tcgatgtgga cagcaaattc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tctccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccatcaccct gcaaaaagcg
     1321 cgcggcaaat acgagcccaa ccgtgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggctcac ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcacaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcagca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgatttcc aacggcagat ggaagcctac tggagccttg tgtacctgca acagcaaggt
     1801 acaagcgagc tgaccgcgac catcctcaaa gaagacctcg tccgcatcga aggcctgccg
     1861 ctggtcacgc gcgcgaccgg tattccgttt gacgcgctgc ccaaatcaca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcactcgcag gcaaaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgata
//
LOCUS       DAXHMX010000213         2193 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXHMX010000213 DAXHMX010000000
VERSION     DAXHMX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.213"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            30..515
                     /locus_tag="ACFM4S_002060"
     CDS             30..515
                     /locus_tag="ACFM4S_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051666.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDALAFVFGRTGLLVSV
                     SDGGFGWRGGWDGVVWFVYGVFAFLNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSGALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            611..1234
                     /gene="rsmG"
                     /locus_tag="ACFM4S_002061"
     CDS             611..1234
                     /gene="rsmG"
                     /locus_tag="ACFM4S_002061"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6051667.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLEAERHIVILSKR"
     gene            1333..2106
                     /locus_tag="ACFM4S_002062"
     CDS             1333..2106
                     /locus_tag="ACFM4S_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6051668.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
BASE COUNT          422 a          503 c          761 g          507 t
ORIGIN      
        1 tcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 tggcatttgt gttcggacgg acggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcgaggagg ctgggacggc gtggtgtggt ttgtgtatgg cgtgtttgcg tttttgaacc
      241 tggtcgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattccc
      301 gttacgcgct gtttctgtcg ggcgtggcgg cttgcgcggc tgctgcggtc gcgtggattt
      361 tcgagctgct gcttggcagt ggggctttgg gcgggctgcg tggggaggcg gtgttggaat
      421 atgcgtttgc cgtgtggctg gtggcgatgc tgacgctgcc caaacgcctg acgcgcgcgc
      481 cggtgcagcc ggtggtgttt cacaggaaaa aataggttgg aacgggaaat gccgtctgaa
      541 ccccgacacg cggtttcaga cggcatgttt ttccgttaac attacgcctg aacatggaca
      601 ggatagggat atggaacgca aagaacgcct gcgtgcaggc atcgccgcat tgggtttgga
      661 tatttccgca gcggcgcagg acaggctttt ggcctatgtg gatttgttga aaaagtggaa
      721 caaaacctac aatctgaccg ccctgcgcga cgaggaaaaa atgattgtcc atcatctttt
      781 ggacagcctg acgctgctgc cccatatcga gggtgtgcaa acgatgctgg atgtcggttc
      841 gggcggcggt cagcccggca ttccggcggc ggtgtgccgt ccggatgtgc agataaccct
      901 tttggatgcg aatacgaaga aaacggcttt tttacagcag gcggttatcg agttgggatt
      961 ggacaatgtg cgcgtggtat ccggacgcgt ggaggcggtt tcggacgtgc gtgccgatgt
     1021 ggttaccagc cgtgcgtttg cagaactggc ggattttgtg tcgtggacgg tgcatctgtt
     1081 gaaagacggc ggctactggg cggcgatgaa gggcgtgtat ccgcaggaag aaatcggccg
     1141 cctgccgcag gatgtgtgcg ttgaaaaagt ccaaaggctc gacgtgccgg gcttggaggc
     1201 ggaacgccat atcgttatcc tgagcaagcg ttgagcgcgc ttcagacggc atgaatacct
     1261 tttttgtgcg gataaaggta aaattccgca ctgtttttct tttttcaaca tcagacggga
     1321 cacgggcggg acatgagtgc gaacatcctt gccatcgcca atcagaaggg cggtgtgggc
     1381 aaaacgacga cgacggtaaa tttggcggct tcgctggcat cgcgcggcaa acgcgtgctg
     1441 gtggtcgatt tggatccgca gggcaatgcg acgacaggca gcggcatcga caaggcgggt
     1501 ttgcagtccg gcgtttatca ggtcttattg ggcgatgcgg acgtgaaatc ggcggcggta
     1561 cgcagcaaag agggcggata cgctgtgttg ggtgcgaacc gcgcgctggc cggcgcggaa
     1621 atcgagctgg tgcaggaaat cgcccgggaa gtgcgtttga aaaacgcgct caaggcagtg
     1681 gcggaagatt acgactttat cctgatcgac tgtccgcctt cgctgacgct gttgacgctt
     1741 aacggcttgg tggcggcggg cggcgtgatt gtgccgatgt tgtgcgaata ttacgcgctg
     1801 gaagggattt ccgatttgat tgcgaccgtg cgcaaaatcc gtcaggcggt caatcccgat
     1861 ttggacatca cgggcatcgt gcgcacgatg tacgacagcc gcagcaggct ggttgccgaa
     1921 gtcagcgaac agttgcgcag ccatttcggg gatttgcttt ttgaaaccgc catcccgcgc
     1981 aatatccgcc ttgcagaagc gccgagccac ggtatgccgg ttatggctta tgatgcgcag
     2041 gcaaagggtg ccaaggcgta tcttgccttg gcggacgagc tggcggcgag ggtgtcgggg
     2101 aaataggtca atccaaatcg ggctgcccgt gcctttatgc tgtttggccg ggtgcgttaa
     2161 tatggcggat taaaataaaa atacttatat cgt
//
LOCUS       DAXHMX010000214         2105 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXHMX010000214 DAXHMX010000000
VERSION     DAXHMX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.214"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..485)
                     /locus_tag="ACFM4S_002063"
     CDS             complement(<1..485)
                     /locus_tag="ACFM4S_002063"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6051669.1"
                     /translation="MKIILTTSMSGLGGTETATVRLGRLLKRRGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NA"
     gene            complement(482..1903)
                     /locus_tag="ACFM4S_002064"
     CDS             complement(482..1903)
                     /locus_tag="ACFM4S_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6051670.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPTDDIGRIVLMQT
                     AAGLTVSALCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFW
                     LFFAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAVYLAG
                     CILRHWKDLHKLFHYLKKQGFPL"
     gene            complement(1846..>2105)
                     /locus_tag="ACFM4S_002065"
     CDS             complement(1846..>2105)
                     /locus_tag="ACFM4S_002065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051671.1"
                     /translation="PTPYWFLLIHYNADKKRPRSDRRPPGKAQPGLPHPAGSRDGLKA
                     VKFQSRKASDGTGSIPPPSGHQKWTQKKSSATRQARSAARF"
BASE COUNT          451 a          555 c          724 g          375 t
ORIGIN      
        1 gcgttgtagg cgtaggcaat ccggccggcg gggaagccgt ggcggatgag tttttcgcgc
       61 tcgtgtttac agttgccgat gatgtacacg cccagcttgt cgaagagctt ggcgattttg
      121 gggtaggttg ccgcgtccaa accgtgcgag tggtagatga ttttggtttt cggcgaaacg
      181 attttggcgg caagggcgca ggctggaaca acgcgcgcca tttggcagtc gatgatgtcg
      241 ggttgttcgc ggcgcagcat ccgggcgtag gcaaaggtgc ttttgaggta gccggcaagc
      301 ccgccccggt aaaaatcaac cggctgccaa cggatgcccg atgcttgcgc ctctccgaca
      361 aaaggcccgt ccgaagaagc aaggatgatg tcgtgcccgc gccgtttgag cagccgcccg
      421 aggcggacgg tggcggtttc cgtgccgccc aagcccgaca tagaagtggt caggatgatt
      481 ttcataatgg aaacccttgt tttttcaaat aatgaaacag tttgtgcaaa tctttccagt
      541 ggcgcaggat gcagcctgcc agatataccg cccatacgcc ggcaaacagg gggtagtttg
      601 ccggtgtgcc gaagcaggtg taggccgccg aggaggtcag gcagagcaat gtgtgcgtat
      661 aaagcggcag gcgtttgagc ggctgccaca ggcggcagga gctttcggtc ttgaaggcaa
      721 aaaacaacca gaatgaggtg gcacaggcaa ccgccgcgcc gcgcgcgccg ccggacggta
      781 cggcaagccc cagcagcagc aggtttgccg ccagcgcgcc caaggtggcg agcgcgatcg
      841 ggcgtgtttt tcggacgacg ttcaaaccga tgccgctgat ttctaccagc gtgcaaaaca
      901 gcggcggcag catacacgat acgacgataa accggacggc agcgtagttt tccggcagca
      961 ggagggaggc gaggggcgag aaaatgccgg tcaggcagag ggcggaggca agcagggcgg
     1021 cggcggattc tgccgttgcc gagaggcggg cgggcggggc gttttcttcg attgcgcgga
     1081 aaatatacgg tgtccagacc gttgaaaaga tgctttggaa caataatgcc gccccgccga
     1141 acgaaatacc catcgaataa acgccgagct gttccagacc ggcatatttt ttcaggaaca
     1201 aacggtcggc ggatgccagc ccccaatagg cgatgctgct cagtgcgatc ggtatgccgt
     1261 agcgcagccc ccggtgcagg acggcgggcg aaaacggtgc gtgccggacg gccttcagac
     1321 ggcatcggtt ttgaaacagc aaaaaggcgg cggcggcaag gtttgccagc gcgtaaacgg
     1381 cggtcaggac ggcggtgttc gccggaaagt gcagcagccc gaccgtcagc ggcagcagca
     1441 gcaggatggc gagcttgggc acgagttgcg cggacgaaaa ggcaagggcg cgcccttcca
     1501 tacgcaaaac cagtaagaga aagcggatgg gcaggaagct cagttcaaac agcaccagcc
     1561 cgatgccggc ggcggcatcg tcgagcgaaa acaggatttc agacggcagg gacgggcggg
     1621 aaagcagcag ggcggctatc gcggcggcag acagcagcgg cggcaggaac agggttttga
     1681 acaaagtgtc tttgtcggcg gcggcatagt attcgcggac gtatgcctga tccagcccga
     1741 ggcacaatgc cgacaccgtc agtcccgccg ccgtctgcat cagcacgatg cgcccgatgt
     1801 cgtcggtggg gaaataccac gacagcagcg gcaggatgat gacggctaaa accgcgctgc
     1861 cgatcgagcc tgccgcgtag ccgaggattt cttttgtgtc catttttgat gtccggacgg
     1921 aggcgggatg ctgcctgtgc cgtctgaagc ctttcttgat tggaatttga cggctttcag
     1981 accgtcgcgg ctgccggcgg ggtgcggcag tccgggttgc gcttttccgg gcgggcggcg
     2041 gtctgaacgg ggccgttttt tatcggcgtt atagtggatt aacaaaaacc agtacggcgt
     2101 tggct
//
LOCUS       DAXHMX010000215         2091 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXHMX010000215 DAXHMX010000000
VERSION     DAXHMX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.215"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..150
                     /locus_tag="ACFM4S_002066"
     CDS             <1..150
                     /locus_tag="ACFM4S_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6051672.1"
                     /translation="FWNQAKRVLRKYNGIDRKSFPLCLKECEFRFNFGTPSQQLKILR
                     DWCGI"
     gene            complement(321..836)
                     /locus_tag="ACFM4S_002067"
     CDS             complement(321..836)
                     /locus_tag="ACFM4S_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051673.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGGGVFGAGGKYSMHIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNARIQ"
     gene            complement(1096..1366)
                     /locus_tag="ACFM4S_002068"
                     /pseudo
     CDS             complement(1096..1366)
                     /locus_tag="ACFM4S_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein frpA"
BASE COUNT          718 a          364 c          281 g          728 t
ORIGIN      
        1 ttttggaatc aggcaaaacg cgtcttgcga aaatacaacg gaatcgatcg taaatctttc
       61 ccgctgtgct tgaaagaatg cgaatttcga tttaacttcg gcacaccgtc tcaacagctt
      121 aaaatcctgc gggattggtg tggaatttag ggctaatcta gtacagcccc gattttttta
      181 tctctagccg aatacagttc aacccaaacg tacccgtctt ggatacaccc cctcttgttt
      241 cagtggcgta ccctgcttcg caaattttac agttatcgca taataatcag ccataagtaa
      301 ccccacattt ctagaaaaat ttactgaatt cgtgcattaa ctactattct tatagaatta
      361 tttatttcat cgtaaagtgg aatatattca ctattatcag aaatctcaaa taaatattcc
      421 ccttcaggca aataaaaatc ataaatatgc atactatatt tccccccagc tccaaatact
      481 ccgcctccta attttctact tctctctgta agcaactcat ctataataag ttttttacca
      541 tcttcttcca ctttaaatat tttaaccgat atattatatg gatagtttaa cttatcatca
      601 atccacctct ccccctgttt tggatcataa tgtataaaat tagttcctaa cgatattgca
      661 taaggtttac caattttctg tttgagttca atagatcttt ttgttttttt accaagagga
      721 aagtctacca caacaggttc tgggctaata gccatataaa aaatagtttc aacaaaactg
      781 cagctagcaa tcaataggca agatattatt aataatattt ttctaaatgc agacataata
      841 aaaatctcca aaaaatccat atcagaatat actaattatt tgcataatta ataaggaaga
      901 gaaaaattca aaacggctat gagatttccg cctgaaaata atgaaataaa agtccatagt
      961 aatgaataat agctttatca gccccccttt aatgcaaaca tagagaatac ttcccttgaa
     1021 gcactatcaa tttctactta actaaaactc tagctctatt ttaaaatttt gtcaaaaata
     1081 gttaaatact tttatttatt ttaaataatg agatacattt atggttattt gttttagaca
     1141 gcctaaaaca aacggggaaa tcgtgttttc ccgaacatgg ctaatggata gcattttgtc
     1201 cgtgaggagg ctgtgcaggt ttgtcagcag ccaacagcga atcgcctgct tgcgcggttg
     1261 tgccgtcttt tttggtgtag ctgccttgtt gagccaaagt gttgccgtta ccaagatttt
     1321 tgtttacatc ttttcgagaa aattggcttt gcatttgcct taacaaagat atttctgcta
     1381 ctcgtgcttt attatttata ttatatttac tctgcatttt tcttacaact tgtctcaact
     1441 gactttgtgt tttttgtaat tcacgttgta ccgattttcc taaactttca tgtttggctg
     1501 cataattggt agaatcagtt gacatttgtg tataaaaatt tttacgaaaa ttttcgtaac
     1561 tcagattaat tacatccgta cttgcagtta taaaatcttg tataaattca atagaaactg
     1621 aattatcatc agatttggct ttgttatttt ccatattgag cctctcttaa tttgaaaaaa
     1681 ttaacataaa catttaaaaa taaaacaaaa ttatataata aaaaaaataa tgagaatata
     1741 aaggataaaa agagagttaa gttatttaat cggacttgtt ctctataaat cataaggcac
     1801 caaataagcc ataataaatt tagtaaataa aaactgctta tacccaactt tcttttcttg
     1861 gaacctatta ttatttcaac tctggatgaa aaaatagaat ttaaaaattt ttttctttga
     1921 tatatcgtta atatctccct agtttttctt aaaaaaatat aatcatatat atactacaca
     1981 acattggtaa taaataaatt aaaagcgaaa ggtaattaaa ttgataattg taaattatat
     2041 aataaacaac atacgaagaa aaaattatta tagatgattt gctatacgaa t
//
LOCUS       DAXHMX010000216         2083 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXHMX010000216 DAXHMX010000000
VERSION     DAXHMX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.216"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(26..349)
                     /locus_tag="ACFM4S_002069"
     CDS             complement(26..349)
                     /locus_tag="ACFM4S_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6051674.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(368..577)
                     /locus_tag="ACFM4S_002070"
     CDS             complement(368..577)
                     /locus_tag="ACFM4S_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6051675.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFFRN"
     gene            complement(700..1497)
                     /gene="truA"
                     /locus_tag="ACFM4S_002071"
     CDS             complement(700..1497)
                     /gene="truA"
                     /locus_tag="ACFM4S_002071"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6051676.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQTALETALAQI
                     AGEFVATTVAGRTDTGVHATAQVVHFDTTAVRPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPKIPEWL"
BASE COUNT          479 a          490 c          581 g          533 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaattgccaa gatgcttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatgg gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagagact acgacacaag gacgtgtctt tttgatttcg cttcctacgg
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaaatgct cctgctcggt ttcgacaagc attgcggcag
      421 cttctgccca tcccctgcga acggtaggac agcttaaaat aatattgccc ttttcaactg
      481 taacagccaa gctgtctact gcccctattt gacccaataa tgatttgggc agaatcacgc
      541 cttgcgagtt tcccattttg cgtatgttga gaatcatata cgtaccctat ttaccagttt
      601 atgtaataac aatgttatta ccttgatgag gtagtgtcaa cattgaaaaa gattgcctga
      661 cagtttgtcc gatttcaaaa tctccgcgac aagaatgttt taaagccatt cggggatttt
      721 ggggcggatg atgccgtatg cctcgggata gtcgacgccg gtcaggtaaa gcccgtcggg
      781 catgaaggtt ggtggggctt tgaggcggct gcgttcttga atcagtgcgg cgaagccttc
      841 gacgctgagc ctgccgctgc cgacataaac gagcgcgccc atgatgttgc gtaccatatg
      901 gtgcaaaaag gcgttgccgt gcaaatcgag gcggacgagt cctgcgcttt gggtaaggtc
      961 ggcgcggtag atggttttga cgggggattt tgcctggcac tcggcggcgc ggaagctgga
     1021 gaagtcttgt tcgccgatca aataggcggc agcccgacgc atcggttcga tgtcgagttt
     1081 gaggtgtgtc cagcctgccc tgtttttgag caggggggaa cggacggggg cggattcgag
     1141 caggtagcgg tagtgtcgtc cgtatgcgtc aaatcgtgca tgaaattcgg gggcgacctg
     1201 tcgagcatgc aaaacggcaa ttccttcggg caggtgggca tttacgccgc gcacccatgc
     1261 ctgttgggga cggacggcag ttgtgtcaaa gtggacgact tgggcggtgg cgtgcacgcc
     1321 ggtatcggtt ctgccggcaa cggtggtggc gacaaattct cctgcgattt gggcgagtgc
     1381 ggtttccaat gccgtctgaa cggtcggtac gccgcccgcc tgtttctgcc agccgtaaaa
     1441 gcggctgccg tcataggata gggttattgc ccagcgttgt ttttgtgcgg tatccatcgg
     1501 atttgggatt cggataaatg ttcagactgc attgtatcgc agattttgca gggaaacggc
     1561 aaacgcccag ggcgagcggc gttgtttggg gagttggggg ggggatgcag ttgctacgaa
     1621 tcgctatcct gtgaattcac cctgtcagga gtgcccgaat cgtcattccc gcgcagtcgg
     1681 gaatctagga cgtaaaatct aaagaaacca ttttatagtg gattaacaaa aaccagtacg
     1741 gcgttgcctc gccttagctc aaagagaatg attctctaag gtgctgaagc acgagtgaat
     1801 cggttccgta ttatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
     1861 cactatatcc gataagtttc cgcaccgaca aaactagatt cccactttcg tgggaatgac
     1921 gggatgcagg ttcgtgggaa tgacgcgaac agaaacctca aatcccgtca ttcccgcgca
     1981 ggcgggaatc tagaccttag aacaacagca atattcaaag attatctgaa agtctgggat
     2041 tctggattcc cactttcgtg ggaatgacgg aatgtaggtt cgt
//
LOCUS       DAXHMX010000217         2027 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXHMX010000217 DAXHMX010000000
VERSION     DAXHMX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.217"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            177..1961
                     /locus_tag="ACFM4S_002072"
     CDS             177..1961
                     /locus_tag="ACFM4S_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFC6051677.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLF
                     ATVQANATDDDDLYLEPVQRTAVVLSFRSDKEGTGEKEGTEDSNWAVYFDEKRVLKAG
                     AITLKAGDNLKIKQNTNENTNDSSFTYSLKKDLTDLTSVETEKLSFGANGNKVNITSD
                     TKGLNFAKETAGTNGDPTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRAASVK
                     DVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKI
                     GAKTSVIKEKDGKLVTGKGKDENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQ
                     TGQADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGW
                     NLDSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMAPQFS
                     SVSLGAGADAPTLSVDDEGALNVGSKDTNKPVRITNVAPGVKEGDVTNVAQLKGVAQN
                     LNNRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGDTYRGEAGYAIGYSSISD
                     GGNWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          623 a          488 c          512 g          404 t
ORIGIN      
        1 ataaaacagt ttccttagat tccacgtccc agattcccgc cttcgcggga atgacgagat
       61 tttaagttgg gggaatttat cagaaaaccc ccaaccccca aaaaccgggc ggatgccgca
      121 ccatccgccc ccaaaccccg atttaaccat tcaaacaaac caaaagaaaa aacaaaatga
      181 acaaaatata ccgcatcatt tggaatagtg ccctcaatgc ctgggtcgtc gtatccgagc
      241 tcacacgcaa ccacaccaaa cgcgcctccg caaccgtggc gaccgccgta ttggcgacac
      301 tgttgtttgc aacggttcag gcgaatgcta ccgatgacga cgatttatat ttagaacccg
      361 tacaacgcac tgctgtcgtg ttgagcttcc gttccgataa agaaggcacg ggagaaaaag
      421 aaggtacaga agattcaaat tgggcagtat atttcgacga gaaaagagta ctaaaagccg
      481 gagcaatcac cctcaaagcc ggcgacaacc tgaaaatcaa acaaaacacc aatgaaaaca
      541 ccaatgacag tagcttcacc tactccctga aaaaagacct cacagatctg accagtgttg
      601 aaactgaaaa attatcgttt ggcgcaaacg gtaataaagt caacatcaca agcgacacca
      661 aaggcttgaa ttttgcgaaa gaaacggctg ggacgaacgg cgaccccacg gttcatctga
      721 acggtatcgg ttcgactttg accgatacgc tgctgaatac cggagcgacc acaaacgtaa
      781 ccaacgacaa cgttaccgat gacgagaaaa aacgtgcggc aagcgttaaa gacgtattaa
      841 acgcaggctg gaacattaaa ggcgttaaac ccggtacaac agcttccgat aacgttgatt
      901 tcgtccgcac ttacgacaca gtcgagttct tgagcgcaga tacgaaaaca acgactgtta
      961 atgtggaaag caaagacaac ggcaagaaaa ccgaagttaa aatcggtgcg aagacttctg
     1021 ttattaaaga aaaagacggt aagttggtta ctggtaaagg caaagacgag aatggttctt
     1081 ctacagacga aggcgaaggc ttagtgactg caaaagaagt gattgatgca gtaaacaagg
     1141 ctggttggag aatgaaaaca acaaccgcta atggtcaaac aggtcaagct gacaagtttg
     1201 aaaccgttac atcaggcaca aatgtaacct ttgctagtgg taaaggtaca actgcgactg
     1261 taagtaaaga tgatcaaggc aacatcactg ttaagtatga tgtaaatgtc ggcgatgccc
     1321 taaacgtcaa tcagctgcaa aacagcggtt ggaatttgga ttccaaagcg gttgcaggtt
     1381 cttcgggcaa agtcatcagc ggcaatgttt cgccgagcaa gggaaagatg gatgaaaccg
     1441 tcaacattaa tgccggcaac aacatcgaga ttacccgcaa cggtaaaaat atcgacatcg
     1501 ccacttcgat ggcgccgcag ttttccagcg tttcgctcgg tgcgggggcg gatgcgccca
     1561 ctttgagcgt ggatgacgag ggcgcgttga atgtcggcag caaggatacc aacaaacccg
     1621 tccgcattac caatgtcgcc ccgggcgtta aagaggggga tgttacaaac gtcgcacaac
     1681 ttaaaggcgt ggcgcaaaac ttgaacaacc gcatcgacaa tgtggacggc aacgcgcgtg
     1741 cgggcatcgc ccaagcgatt gcaaccgcag gtctagttca ggcgtatctg cccggcaaga
     1801 gtatgatggc gatcggcggc gacacttatc gcggcgaagc cggttacgcc atcggctact
     1861 ccagtatttc cgacggcgga aattggatta tcaaaggcac ggcttccggc aattcgcgcg
     1921 gccatttcgg tgcttccgca tctgtcggtt atcagtggta agggcttttt cgcctgtctg
     1981 ctgttggggc aggcggaagg tttgaaggga agggtggcga tttgccg
//
LOCUS       DAXHMX010000218         2006 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXHMX010000218 DAXHMX010000000
VERSION     DAXHMX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.218"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            301..1758
                     /locus_tag="ACFM4S_002073"
     CDS             301..1758
                     /locus_tag="ACFM4S_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6051678.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGATTGFLLLLLVPKLNKMLEGTD"
BASE COUNT          451 a          601 c          463 g          491 t
ORIGIN      
        1 atagtacggc aaggcgaggc aacgccgtac tggtttttgt taatccacta taaaaaaccg
       61 ccctatcgga taagccctta acagaaaagg ctttacccgc gccgtatcgg aacacatcct
      121 ctaaaataca atccgttgaa ttgaaaaaaa tataaaaaca tccgcccgcg aaaaacggca
      181 gcgcgtcgtt tgacaaagaa tgaaaatatc ggttaaaaac cgattttcat acaaaaaaca
      241 ccgctgccgt ccgcatccgt ttcagacggt attgagagaa aatcttttag gagaaccttt
      301 atgtcccggc atcccgcccc caccggagaa aaaacattct tcggccaccc cttccagctt
      361 tccaccctct tccatatcga attgtgggaa cgtttttcat tttacggaat gcagggcatc
      421 ctgctgattt acctctacta caccgccgac aaaggcggct tgggcataga caaaaccctc
      481 gccggcggca ttgtcggcgc atacagcggc agcgtgtacc tgtccaccat tttgggggcg
      541 tggtttgccg accgagtatg gggtgcggaa aaaaccctct tcctctcggg catcgtcgtg
      601 atgctcggac acatcgtcct tgccgccgcc ccgggcctgt acggtctttt aatcgggctg
      661 atattcatcg cattgggcag cggcggtgtg aaatctacgg ccagttctat ggtgggcgca
      721 ttatacgaac aggacgaaat gcgcccgctg cgcgatgcgg gattttccat tttctacatc
      781 gccatcaaca tcggcggctt cctgggcccg ctgctgaccg gcctgctgca ggaaaacatc
      841 ggtttccatt atggtttcgg cgcggcggcg gtcggtatgg cattcggctt gtggcgttat
      901 tccttgggac gtaaaaacct gccccacccc accgtccccc atccgctttc aaaaggacag
      961 ggcaaaactg cggccgccgt cggcatcacc ctcatcgccg cacttgcaac cgccatcaaa
     1021 accgggcttg tcaacctcga caatttctcc ggcatcctct tatctaccgt catccttgcc
     1081 gtcattgcct atttcgcccg cctgctgacc aacccccgcg tcagttccga caacaaacgg
     1141 cacatcatcg cctacatccc gcttttcctg accatctgta tgttttgggc cgtctggttt
     1201 cagatttaca ccgtggcaac cgtctatttc gacgaaaccg tcaaccgcac catcggttcg
     1261 tttaccgtgc ccgtcgcctg gaaagattcc atgcaaagcc tgtgggtcat cctgttttcc
     1321 ggactgatgg cggcaatgtg gacaaaaatg gggcgcaaac agcccaaaac cccgctgaaa
     1381 ttcgctatgg cggtatttgt taccggcgcg tcgtttttgg gattcgtccc ctttatttcc
     1441 gccggcacgc cgatgcctat tgcggttttc gcactgattg tccttgccat cacgataggc
     1501 gaactgatga tttccccgat tgcgctgtcc atctccacca aaatcgcacc gcctttattc
     1561 aaaacccaaa tggtcgccct taatttcctt gccttttcat taggcttcac tttgggcggc
     1621 gtattgtttg agaaaggcta tcaggcgggc gacgaaatcg gcttctaccg gctgctgttc
     1681 tacatcggcg caaccacagg cttcctgctg ctcctgctcg tccccaaatt gaacaaaatg
     1741 ctcgaaggca cagactaagt cccgccccga tgcgccgatg ccgtctgaac ccttcagacg
     1801 gcatttttcc gcataatgaa accaaaccgt ttccacccga caggacaggc tccccccaac
     1861 cggaaggcag cctgccgatt gtcatttgaa tatctgactg attccgtctg tttgcaaggg
     1921 aaatagccgt ttatttccgc ttgggcggaa accggttcta ttgagtaaaa ggcattttat
     1981 ccgactaaat tagtgctgca tcaacg
//
LOCUS       DAXHMX010000219         1963 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXHMX010000219 DAXHMX010000000
VERSION     DAXHMX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.219"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..287)
                     /locus_tag="ACFM4S_002074"
     CDS             complement(<1..287)
                     /locus_tag="ACFM4S_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051679.1"
                     /translation="MKKDIFYCEQWSYGYKKLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRD"
     gene            complement(284..>1963)
                     /locus_tag="ACFM4S_002075"
     CDS             complement(284..>1963)
                     /locus_tag="ACFM4S_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6051680.1"
                     /translation="YIADIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAY
                     DKWDYKQEGMTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTG
                     VAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSS
                     TVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAI
                     NGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGK
                     CRDGAIGAAVGEMVGETLLDGRDVGKLSPQERQKVIAYSQIIAGSVVALVKGDVNTAA
                     NAATVAVENNSLLARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAILR
                     TPGEPWTRHDVQTYRQYYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYNELYV
                     ANPKSATGYFRVDSYNPATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNV
                     PSTPTTIRGRKLEGKLILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
BASE COUNT          419 a          479 c          450 g          615 t
ORIGIN      
        1 taatctctcc atacagccat agaaagaaaa taataattcg aattggaata tttttgaaat
       61 tgatgggtta aataatccct tccaaattta tcgaaaaaat gtaccgaaaa aaaacctact
      121 tcctctcgca aggtaattac atattcaggt tgtgtcgccg aacctattac ggcagtatat
      181 aactcccctt taagatgttt ttcctcagct tgtttctcag aaaaaggctt atgaagtttc
      241 ttataaccat aagaccactg ctcacaataa aaaatatctt tcttcatttg taaatccttc
      301 ctgttgtatc tctaatgata acattttctt cttgtgccgc ccttaataca gattgtggaa
      361 ttggattgac ctgagcagga acttctaaaa taagttttcc ttcaagcttt cttcctctta
      421 tcgtagtagg tgtacttgga acattggaaa tgacagcacc agggctatat tttctaacag
      481 cctccttgat ataaccaatc cccgtacttt cttggatttg agaaaattgg gtaaattttc
      541 ttgaaataat ttcctctgtc gcaggattat acgaatctac cctaaaatac cccgtcgccg
      601 atttaggatt ggctacataa agctcattat aagggtattt tgatgacatg atacggttaa
      661 attcattgcc gttgtttatc ctgattctat aaattgggtc aacagcaaag cctctggatt
      721 cccttaattg attataatat tgcctgtatg tttgtacatc atgtcttgtc cacggctctc
      781 caggagtcct cagaatagca atcccgttaa atttcggatc cagccttcgg atttgattgg
      841 taatggcctg gatttcaaga atggcatatt catgttccaa ttcttgtcgc ggagtccaac
      901 gtatatttac cctcctgcga gctaaaagac tattattctc cactgccaca gtagccgcat
      961 tcgccgccgt attcacatcc cctttaacca atgccactac gctgcctgcg ataatctgcg
     1021 agtaggctat gactttttgg cgttcttggg gtgacagttt gcctacatcg cgtccgtcca
     1081 acagggtttc tcccaccatc tcgccgactg ccgcgccgat tgcgccgtcc cgacatttgc
     1141 ctttatttgc taccgccgat gcacagcctg ctacggcatg ggctatcttg tgggcaatgt
     1201 agtcttcgct gagattaaat ttgattttgc tcgctacttc tccgtgtacg gtactgacta
     1261 tcgcacccag tgcggcatcg cccaagttgt cttttaggct gccgccgttg atggcggtat
     1321 ggacacttgc ggcagcggtg ctgttgatca ggttagcggt cagtttgcct gctgcgggac
     1381 tgtgaaaatg tttgctgacg gcttcggctg cttgggtgtt cagcccgctt atgccctgca
     1441 gtacgcctgc ggttacggcg gcggtggcgg cctgtctgac ggtgctgctt ttgcccagtt
     1501 ctttcagggt atggtttatg tctcctttgt tgttgatgag ggaaactgcg gcttggctgg
     1561 cgagactggc cagtgcggct ttgcctgcgg ctgtggtgat agctgcagct gatgtgcctg
     1621 ctgctactcc ggttgtggct gccgttcctg ctgctactcc agttccggct gccgttcctg
     1681 ctgcggctcc tcccgctcct gcgcctgccg cgcccgccgt tccggcggct gccggggcgg
     1741 acagtgcgcc gtaggtcaat acagttacga cgataacgac gacagctgct gctgcgggtg
     1801 tcatgccctc ctgtttgtag tcccatttat cgtaagcaag ctgcacctga ttccagttga
     1861 tgtttttcgc aacttgaagc tgttttaaat aagcatactc gggctgtttg gcgagttttt
     1921 cgatttcggt tttgaggttg cctttgggga tgtcggcgat ata
//
LOCUS       DAXHMX010000220         1883 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXHMX010000220 DAXHMX010000000
VERSION     DAXHMX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.220"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(127..1437)
                     /locus_tag="ACFM4S_002076"
     CDS             complement(127..1437)
                     /locus_tag="ACFM4S_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6051681.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKSLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPYVENGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLGAAETR
                     RLKTLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRALQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDCLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1692..1883
                     /locus_tag="ACFM4S_002077"
                     /pseudo
     CDS             1692..1883
                     /locus_tag="ACFM4S_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213469.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          454 a          494 c          480 g          455 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagca
      241 gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcattgcgca ggtggacggg
      361 tacttcgtcg ctggcgtttt ctttgacgaa acggcgcatt tgattgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt gcaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcagtca gggttttcag
      781 acggcgcgtt tcagcggcgc ctaacagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaaggattg
      961 caaaacatac acctgcgcgc ggctcaacag cgcgggattc acttcaaacg acggattttc
     1021 cgtcgttgcg ccaataaagg tcagcaaacc gttttccaca taaggcaaaa acgcatcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgccc tcccctgctg
     1141 caaagcgatt tcagccttgt cgattgcccc gcgtatgtct tttacgccgg aaaacacggc
     1201 ggaaacaggc agaaactggg cattgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccccctt ctaccgccac
     1321 gcgcaaagat ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcaccggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gattcaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aaaagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 tgaaaacttt agaaaattag aac
//
LOCUS       DAXHMX010000221         1855 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXHMX010000221 DAXHMX010000000
VERSION     DAXHMX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.221"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            212..1684
                     /gene="pyk"
                     /locus_tag="ACFM4S_002078"
     CDS             212..1684
                     /gene="pyk"
                     /locus_tag="ACFM4S_002078"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6051682.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          482 a          537 c          500 g          336 t
ORIGIN      
        1 ccaaaaataa atttagccta agtcataaaa ccgtgtaatt ttgcaccaaa aaagacggtt
       61 tgcaggtaaa atatcaaact attaatttaa atcatatttt tacagaatat tccgccgcat
      121 tcatcaaatg gacatcaaac cggttccaaa tttctgtaat tttgcaacaa aataccgcaa
      181 aacacccgat tgagaccgaa aggactttca tatgaaccag acaagccgcg atctgacccg
      241 catcagccac aacaccaaaa tcgtcgccac cttggggccg ggcagcaaca acgtcgaact
      301 gttggaagac atgatccgcg tcggcggtct gaacgtcgtc cgcttcaact tcagccacgg
      361 cacgcccgaa ttccatcagg aaaacgcccg cattgtgcgc gaggcggcaa aacgcgccgg
      421 acaggaaatc gccatcattg ccgacctgca agggccgaaa atccgcgtgg gcaaaatcgc
      481 cggcggcggc atcgaattga acaaaggcga aacgctggta ctggatgccg cgctcgaagg
      541 cgaaggcacg cgcgaggcgg tcggtttgga ctaccgcgac ctgcccgacg acgttaccgc
      601 aggcgacgta ttgtggctgg acgacggcct gctgaccctg accgtggaat ccgtcgaagg
      661 cagcaggatt atcacaaggg tggaaaacag ccatgtcctg aaaagcaaca aaggcatcaa
      721 caaacgcggc ggcgggctgt ccgcaggcgc gttgaccgaa aaagacttcc gcgacctgaa
      781 aaccgcgatt gccatcggtt gcgactacct cgccatcagc tttgtgaaat ctgccgaaga
      841 tttgcacatc gcgcgcgcca aagtcgaaga agaaatgaag ggcagcactg ccgtgcgccc
      901 cggtttggtt tccaaaatcg aacgcgtaga agcgattgaa aaccttgatg aaatcatcct
      961 cgccggcgac ggcattatgg ttgcgcgcgg cgacttggcg gtcgaagtcg gacacgccgc
     1021 cgtccccgcc ctgcaaaaac ggatgatccg ccgcgcccgc gagttgcgcc gcttcagcat
     1081 tacggcgacg caaatgatgg aatcgatgat taccaacccc gtgccgaccc gcgcggaagt
     1141 cagcgatgtg gcaaacgcgg tattggacgg taccgatgcg gtgatgtgtt ccgccgaaac
     1201 cgccgtcggc gcgtatccgt ttgaaaccgt cagccaaatg gcaattatct gcgcggctgc
     1261 ggaaaaagag caggattcgc tcaacggcgt tgccgaacag gcggagtatc ccgaagcggt
     1321 cagcaccaac ctagcggttg ccggcggcgc ggtcagcgtg gcgcgcgcgg ttcacgccaa
     1381 agcgattgtc gccctgaccg aaagcggttc gaccgccttt gaaatcagcc gccacaacat
     1441 caccctgccg attttcgcgc tgaccccgag cgtttccgcc caacgccgta tggcgatgta
     1501 ccgcggcgtg cgcccgctga ttttggcaac cagtaccgac cacgacacgg cgttgaacga
     1561 agtcgaaacc atgctggtgg aacacaacat cctgcattcc ggcgaccaat acatcatcac
     1621 cagcggctcg caaatgcgcg aatccggttc gaccaatacg ctggaagtgc tgcgcgtcaa
     1681 ataatccgcc ctgagtggaa aatgccgtct gaagtcgatg cccgaggctt cagacggcat
     1741 tttttgcggc ggcgcggcgg ttcaggcaaa ctgaaaaaat tgtgcaaatc cggcaacatc
     1801 agataaaatt gagtacctat actaaagcga aacaaggcat ttccgactgc ctttt
//
LOCUS       DAXHMX010000222         1852 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXHMX010000222 DAXHMX010000000
VERSION     DAXHMX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.222"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..202)
                     /locus_tag="ACFM4S_002079"
     CDS             complement(<1..202)
                     /locus_tag="ACFM4S_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051683.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNR"
     gene            complement(199..744)
                     /locus_tag="ACFM4S_002080"
     CDS             complement(199..744)
                     /locus_tag="ACFM4S_002080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6051684.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            complement(901..1329)
                     /locus_tag="ACFM4S_002081"
     CDS             complement(901..1329)
                     /locus_tag="ACFM4S_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6051685.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(1310..1744)
                     /locus_tag="ACFM4S_002082"
     CDS             complement(1310..1744)
                     /locus_tag="ACFM4S_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC6051686.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          447 a          534 c          380 g          491 t
ORIGIN      
        1 gacgattctt gcgtttgcgc gccgcgcgtt tggctgccgc cacgcctgag tttcttaaat
       61 ttgggcgtgg ataggtgtgt atcggacgaa catttggcat ctgtataagt gcgcggttta
      121 cctgctcatt actaggattt tctccaatca attttgtaag aaaataggaa gcagcttctt
      181 tcaatttacc aataatattc atactcttgc cgcccccaat tccatcgcaa tcgcgtccgc
      241 aatcgcgcgg cagatgcccc atttagtagt cttaaacaag gctaaatcag tgtcgttgct
      301 gataaaaaac ggctcaaaca caatgccgcc ggcttgtgca taagccaggc gcgaatgctg
      361 gcccgcattg tcgggtttaa agccgtcttc gccgcgcagt ttccagccgg ttttcttggc
      421 aacagctttg ctcaacacct gacaccagcg tttgttttta acggtactca aggcttcgat
      481 gcctgtcgcc gctttgctga cggcagcgtt ggtgtgaaac tcaatcgcca catccgagcc
      541 gcgaatcagc ttgaccgcat cgcgcaaggg catattgcct ttgccttcgc cgtcggtgcg
      601 tacggtcagg ccgtaatcgt tacgcaggat tgaagccacg atgttgcgca tatcctgcgc
      661 caagtccgcc tcacggtcgc ttccgttgac cgcgcccggg tcggtgttac tgtgtccggc
      721 ggttaaagtt acagttttgc ccattcatca tctccaaaaa atattgattg cataacattt
      781 gaaaacccca ttaaaccgtc tttaacccat cgtcccgaac ggtagatgtt tcatccccta
      841 caaaccaaaa aaaaagaccg tctgaataca gacggccaaa gcctggtcac acatcactgc
      901 ctaaaataaa gctgcctgct gtgccgcagg tcggctcatt tcattgataa tcgtatatcc
      961 tgttcgtgaa gagataccgt atttagggca tagcttcgtc atcgccataa gcccgctctc
     1021 cttatcaata tcgcgcaatt tgacaaactc ctgataaaac ctatggtttc tcaactgtat
     1081 taacgccttg ccgcaccgtg ggacatacaa ttcctcgcca ccatatacct gcaacagctc
     1141 atgtgttttc acttcgccga tggcttcgac caaaattgcc aaacgctcgg tgtccacctt
     1201 gcccttacca aatttaaacc gcgccccgcc aatcgccttg accagctgtt ccgtcgctgc
     1261 cagtccgatg acatccacaa tgtccaacac ggtatccggc aataaatgtt caactttttc
     1321 gaaccccatc atccccaccc gctttttccg ttttcctgtt ttccgcaatc tgcaacgcag
     1381 caaccagttt atgtagctgc gtatcgtcta aatattcgac cttatcctta ccaaacatcc
     1441 gccgcgccat tgcgtgtgca tagttccaat gtttgccgcc gacggtcagc agggcttcga
     1501 ctttgtccaa cattgccgct gatgatgtcc gacgcagatg cggtttgccg tgtgggttac
     1561 cttttgcttt aggcttaaat ccgtgcgacc gcatatcagc gacaacagac tcaagttcgg
     1621 aaacatccat atccgcacac gaccgcttgc ccgtcacgcg ctccaacacc gcgcgatagg
     1681 taccatcgtc caagcccagc tccttttggg caattttaat ttttgcaatc aacgcccggc
     1741 gcatctcaaa cccataaaac acaatatatt tactaattaa cgcatattat accaataaaa
     1801 tacaatatat agtattaagc cgctgttttt tgcgaaacgg acagacataa aa
//
LOCUS       DAXHMX010000223         1835 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXHMX010000223 DAXHMX010000000
VERSION     DAXHMX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.223"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..243
                     /locus_tag="ACFM4S_002083"
     CDS             <1..243
                     /locus_tag="ACFM4S_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6051687.1"
                     /translation="LVLIFVNPLHMELETVVPPEEGEGEGEGGDGEIPEYLELHSERE
                     DDISAPSQAEPPPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            278..1789
                     /gene="ubiB"
                     /locus_tag="ACFM4S_002084"
     CDS             278..1789
                     /gene="ubiB"
                     /locus_tag="ACFM4S_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6051688.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAVWVERLFADGKRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT          405 a          495 c          496 g          439 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactacat atggagttag aaacggtcgt gccgccggag
       61 gaaggggagg gcgaaggaga aggcggggat ggggaaatac ccgaatatct tgaacttcat
      121 agcgaacggg aagatgatat ttccgcacct tcgcaagccg aaccgccgcc ccgcaatatc
      181 aaaccgctgg ttttcgggct gctgatagcc tttttgggct atctgctcgg taagattttt
      241 tgattgtccg ataaatgctg tattcgggat tttatatatg aaatggttga aacgcctgac
      301 ggttattgtc gggacttttt accgctatcg gctggcaggt ctgtgcgctt cgctgatggg
      361 tagcggttgg atatgcgctc tgctgaaaat gatgccgcag tcgtccaaat tgaaaaacga
      421 accgcctgcc gtccgtctgc gccttgcctt ggaaagcctg gggccgattt tcatcaagtt
      481 cgggcaggtt ttgtccacac gccctgattt gattccgcac gattacgccg tcgaactggc
      541 aaagctgcaa gacaaagtgc cgccttttga cgcgcggctt tcgcgtgaac aaatcgaaaa
      601 atcgttgggt cagtccatcg aaaagctgta tgcggaattt gaaaccgagc ccgtcgccag
      661 cgcgtccatc gcccaagtac acaaagcccg cctgcattcg ggcgaacgag tggctgtcaa
      721 agttttgcgc cccaaccttt tgccccttat cgaacaggat ttgtcgctga tgcgtttcgg
      781 cgcggtttgg gtggagcgtc tgtttgccga cggcaagcgt ctgaagccgc tcgaagtggt
      841 ggcggagttc gacaaatacc tgcacgacga gttggatttg atgcgcgaag ccgccaatgc
      901 cggacagctc gggcgcaatt tccaaaacag caatatgctg attgtgccca aggtgtttta
      961 cgactactgc accagcgacg tgctgacaat cgaatggatg gacggcacgc cggttgccga
     1021 catcgccaaa ctcaaagcag acggcatcga tttgcacaaa ctcgccgatt acggcgtgga
     1081 aatctttttc acgcaggtct tccgcgacgg ctttttccac gcggatatgc accccggcaa
     1141 tattttggtt gccgccgaca accgctacat cgccctcgat ttcggcatcg tcggcacgct
     1201 gaccgattac gacaaacgtt atctcgccat caatttcctc gcctttttca accgcgatta
     1261 ccggcgcgtc gccaccgccc acatcgaatc gggctgggtg cctgccgaca cgcgtgcgga
     1321 agaattggaa gccgccgtcc gcgccgtgtg cgaaccggtg ttcaacaaac cgatttcgca
     1381 gatttccttc ggcttggtgc tgatgcgcct gtttgaagtc agccgccgct tcaatgtcga
     1441 aatccaaccg cagcttgtgc tgctgcaaaa aacgctgctc aacatcgaag gcttgggccg
     1501 ccagctcgat cccgatttgg acttgtggaa aaccgccaaa ccgtttttgg tgaaatggat
     1561 gaacgggcag gtcggcccta aagccctttg gcgcaacctc aaaaacgaag cccccgactg
     1621 ggcgcaaatc atcccttcat tgccgcgcaa aatcagtgcg ttgattgatg aaaaccgcca
     1681 gcaggaaatg cgtgatgcct atatccattt ggtcaaagtg cagcagcggc aaagcctgtg
     1741 gctggctgtg attgcggttg ttttgctgct gattttgctt ttgaaatagg ctttgtccga
     1801 atcatcgccc gactccgccc gtttataagg aaatc
//
LOCUS       DAXHMX010000224         1830 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXHMX010000224 DAXHMX010000000
VERSION     DAXHMX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.224"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..98)
                     /locus_tag="ACFM4S_002085"
     CDS             complement(<1..98)
                     /locus_tag="ACFM4S_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6051689.1"
                     /translation="MSTFFQQTSQAMIAKHIDRFPLLKLDQVIDWQP"
     gene            154..567
                     /locus_tag="ACFM4S_002086"
                     /pseudo
     CDS             154..567
                     /locus_tag="ACFM4S_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fe(3+)-pyochelin receptor"
     gene            710..1768
                     /gene="dinB"
                     /locus_tag="ACFM4S_002087"
     CDS             710..1768
                     /gene="dinB"
                     /locus_tag="ACFM4S_002087"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6051690.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCASLLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT          432 a          491 c          505 g          402 t
ORIGIN      
        1 ggctgccaat caatcacttg atccaacttc aatagaggga agcggtcgat gtgtttggca
       61 atcatggctt gggaggtttg ctggaagaag gtgctcatgg aaaatcccct aaatggcttg
      121 gtgggaattt aggggatttt ggggaatttt gcaaaggtct caggttatac ctacaacaag
      181 agcaggtata ggaatgcagc cgaagtcaac gccgaacgcc ttgccaaaaa ttccagtgca
      241 gacccgtaca acttcagcaa tttcacaccc gtgcatatat tccgtttcgg aacgagtttc
      301 catataccga atacggggct gaccgtcggc ggcggcgtgt ccgcacaaag cagcacaagc
      361 agcctgtaca acatcaggca gggcggctac gggctgatag acggtttcgt ccgttacgaa
      421 ttgggcaaac acgccaaatt gagcctcatc ggtacgaact taaacggacg cacttatttt
      481 gagaacaact acaaccgtac gcgcggcgca aacaacttct atggagagcc gcgcactgtc
      541 agcatgaaac tggattggca gttttaattg aaaaccgtag ttttgcggat gccgtctgaa
      601 cagggtatgt ttcagacggc atttttatgg aggcaggcct atcggggcgt atatttggaa
      661 tttgctcgat gccggcagta gaatatcctt tttatcttca gcagagccga tgtcttcacg
      721 caaaatcatc cacatcgaca tggacgcgtt ctacgcatcg gtagagctgc gcgaacagcc
      781 gcatttgaaa gggcggccgg tggtcgtcgc gtgggagggc gcgcgttcgg tgatttgcgc
      841 cgcatcgtat gaggcacggc agttcgggct gcattccgcg atgtcggtgg caacggcgaa
      901 aaggctgtgt ccgcaagcgg tgtatgtgcc gccgcatttc gatttgtacc gtcaggtttc
      961 cgcgcagatt cacgccgtat tcaggcgtta taccgattta atcgaaccct tgtcgctgga
     1021 cgaagcctat cttgacgtta cccgtaattt caaaaacatc ccttacgcca gtgaagttgc
     1081 caaagaaatc cgtgccgcca tttttgcgga aacaggtttg actgcatccg caggcatcgc
     1141 gccgaacaaa tttctggcga aaatcgcgtc ggactggcgc aagccgaacg ggcagtttgt
     1201 gttgccgccg cacaaagtca tggcattttt ggaaaccctg cctttgggca aaatccccgg
     1261 cgtgggcaag gtaacgctga aaaaaatgca gtcgctgggt atgcggacgg cgggcgactt
     1321 gcgccgtttc gagcgcggcg agctcttaaa ccatttcgga cgctacggct accgcctcta
     1381 tgatttggcg cgcggtacgg acgaacgccc cgtcaaagcc gaacgcgaac gcctccaaat
     1441 ctccacagaa attaccctgc ccgaagacct gccgctcgag caggctgccg gacacctccc
     1501 ccatcttgcc gaagacttgt ggcggcaaat cacgcgcaaa aacgtcgaag cccaaagtgt
     1561 aacgctcaag ctgaagacct acgatttccg catcatcacg cgcacgctga cttattcctc
     1621 cgtattgccc gactgcgcct ccctgcttca ggcggcgcaa atgttgatgg cgcgcgtccc
     1681 gccgcagacg gaagacgcgt tccgccttat cggtatcggc gtggggcatc ttgtgccgaa
     1741 aaaccagcag caggatttgt gggcgtaaac cgctttacgc gcgccgtcca aaaaatatgc
     1801 cgtctgaacg gttttcagac ggcatggctg
//
LOCUS       DAXHMX010000225         1825 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXHMX010000225 DAXHMX010000000
VERSION     DAXHMX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.225"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..1717
                     /locus_tag="ACFM4S_002088"
     CDS             <1..1717
                     /locus_tag="ACFM4S_002088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051691.1"
                     /translation="NEHFEQDLIPYAVRFRLNQILPELDCHWQENLTEIFKTLSPEAG
                     QAACTQILSRKGIGYNEADGSFTYLPQKRISFSSLATDIPIPDLKRLAGHIAERLDVL
                     AQCESPVYIADILESVLSVIKNVDTGGKRARENFKQILKTEFIYAAAAVLKNKTKWEI
                     PENTRNLDSDIICTYICEVFLKSHLLGNSFPMMRPREVKQMPEPVFDKVLFAEQRARQ
                     LEVIRTSKYIFAIAPYYTDDIPFSLRRFLSEDKLYTSYNRYYTAIHIPVRNITQNDAL
                     RFANGLKQILSLQSGVSWEIIDMMDRIEENYDKKALPLLFSPFPAQVERTQAIAARLD
                     QFERLLGDTVLDPFYYCLTRMAKGEEDLKYIYIAFRQSFGGIFNSFENFRLLPALWMS
                     RDAENMSDRMNAYISAMEDRRREILSIRQGKPEEEFSRKMVVCLIDLENCLEKHLEQF
                     KPVSESIEACTAKLQEQPSFFNRLMKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLY
                     RHREVVSVHNRKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIF
                     SWIPR"
BASE COUNT          476 a          509 c          426 g          414 t
ORIGIN      
        1 aaatgaacat ttcgagcaag accttatccc ttacgccgtc cgtttcaggc tcaatcaaat
       61 ccttcccgaa ctcgactgcc attggcagga aaatctgaca gaaatcttca aaacactttc
      121 tcccgaagcc gggcaggccg cctgtacgca aatcctttcc cgaaaaggca taggctataa
      181 cgaagccgac ggttctttta cttaccttcc ccaaaaacgc atttcgtttt cttctttggc
      241 aacagacatt cccatccccg acctcaaacg gctggcgggg catattgcgg aacgcctgga
      301 tgtattggcg caatgcgaat cgccggttta tattgccgac attctcgaaa gcgtgctgtc
      361 cgttatcaaa aacgtcgata ccggaggcaa gcgcgcacgg gaaaatttca aacagattct
      421 gaaaaccgaa ttcatctacg ccgccgctgc ggtattgaaa aacaaaacca aatgggaaat
      481 ccccgaaaat acccgcaatc tcgattcgga catcatctgt acctatatct gcgaagtgtt
      541 cctaaaatcg cacctgctcg gcaacagttt tccgatgatg cgcccgcgcg aagtgaagca
      601 gatgcccgaa ccggtgtttg acaaagtatt gtttgccgaa cagcgcgccc gccagttgga
      661 agttatccga acgtcaaaat acatcttcgc catcgcgcct tattacaccg acgatattcc
      721 gttttccctg cgccgcttcc tgtcggaaga caaactttat accagctaca accgctatta
      781 caccgccatc catatccccg tccgcaacat cactcagaat gatgccctgc gttttgccaa
      841 cggtttgaaa cagattctct cgctgcaatc cggcgtgagc tgggaaatca tcgatatgat
      901 ggacaggata gaggaaaact acgacaaaaa agccctgccg ctgttgtttt cgccattccc
      961 cgcgcaagtc gaacgcacgc aggcgattgc cgccagattg gaccaattcg agcggctgtt
     1021 gggcgacacc gtcctcgatc cgttctacta ttgcctgacg cgtatggcga agggcgagga
     1081 agatttgaaa tatatttata tcgccttccg ccagtctttc ggcggcatct tcaattcgtt
     1141 tgaaaacttc cgcctgctgc ccgcgctgtg gatgagcagg gatgcggaaa atatgtccga
     1201 ccgcatgaac gcctatattt ccgcaatgga agaccgccgc cgcgagatat tgtccattcg
     1261 gcagggaaag cctgaagaag aattttcccg caagatggtc gtctgcctga tcgacttgga
     1321 aaattgtttg gaaaaacacc tcgaacagtt caagccggta tcggaaagca tcgaagcctg
     1381 taccgccaaa ctgcaggagc aaccgtcgtt cttcaacagg ctgatgaaaa ccaacgacaa
     1441 gctcaacagg cagattgacg tgttgcagaa acagtcggca gccatccaca acgaagccta
     1501 tatcgaaatg aacacgctgc tttaccgcca ccgcgaagtg gtttccgtcc acaaccgcaa
     1561 agcagattat gcggaaaagg gcaaagagca gattgcgctg ttcccgcgcg gattgaacgg
     1621 catcaccaag ctgcccgccg ccgtcctgtt gcccgagcgt ccctaccatt ttgatatgaa
     1681 ggaggttctg catatattct cctggatacc gaggtaggcg tatcggctgc cggataaaaa
     1741 tgcaccccgt cattcctgtc tgtacggggg tagcggcagt gttttcctgc ttcgttttat
     1801 agtggattaa caaaaaccag tacgg
//
LOCUS       DAXHMX010000226         1816 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXHMX010000226 DAXHMX010000000
VERSION     DAXHMX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.226"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(194..1363)
                     /gene="porA"
                     /locus_tag="ACFM4S_002089"
     CDS             complement(194..1363)
                     /gene="porA"
                     /locus_tag="ACFM4S_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin PorA"
                     /protein_id="HFC6051692.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGASGQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGG
                     ATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDM
                     PVSVRYDSPDFSGFSGSVQFVPIQNSKSAYTPAHVVVNNKVATHVPAVVGKPGSDVYY
                     AGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSATSDQAKGTDPLKNHQVHRLTG
                     GYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDLIERG
                     KKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          464 a          467 c          414 g          471 t
ORIGIN      
        1 tatatgtgca aaccaaaccg aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tactgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgagcagatt
      121 ggcagtcaga ttgcaatcga agaatgaatg caagccgtca aaaacaaagc tatccgcttc
      181 accgccccga tatttagaat ttgtggcgca aaccgacgga ggcggcatta atttgagtgt
      241 agttgccgat gccggtattg cgtttcagcc aagcgccaga cacgatggcg gaagtgcgtt
      301 tggaaaaatc ataatcaacg ccggcgatga tttgatcgta gctggtattt tcgccttttt
      361 taccgcgttc gatcaagtcg aaaccatggg catagctgat gcgtggaact gcattaccga
      421 agcggtagga agcagtcgcg gcaatttcgg tcgtactgtt tttggttttg tcggcatttt
      481 cagacaaatc caactgagcc gccaaggcga gattcaagcc gccttcctca tagccgcccg
      541 tcaggcggtg tacctgatgg tttttcaagg gatcggtacc tttggcttga tcactcgtcg
      601 cgctgccgat caagaacaac tcaaaagcat tacgtccgac attggcgtgt ctcgcatatt
      661 taaaggcata gttcccggca aaaccgccat ttttgtaatt cagaccggca taatacacat
      721 ccgatccggg cttgccgaca acagccggaa cgtgagtagc aaccttgtta ttcacaacaa
      781 catgagccgg cgtataggcg gacttgctgt tttggatcgg aacgaattga acgctgccgc
      841 tgaaaccgga aaagtccgga gagtcgtagc gtacagaaac cggcatatcg tcgtggcgtt
      901 tgaaaatacc caattgcgaa gccacatcat tgttgctgtc ccaaggatca atggcttggc
      961 tggcatcgtc aaactgattc gcaacgcgac cggcgcgcag cgtaccgaat tcgcctgcca
     1021 agccgataaa ggattccctg ttgccccact gggtcgcgcc gccgccggca acggatacgt
     1081 cttgctcaag ctgccaaaca gctttcagac cttcgcccaa atcctcgctc cccttaaagc
     1141 cgataaacga gccgaaatca ctgattttcg tcctgatgcg gcttttggcc ttagtaactt
     1201 ttacctgacc gctcgctcca ccgttagcgg cttgtgcttc agtcaattgc agctggtagt
     1261 tcctgccttc cacgccggct ttgatttcgc cgtataggct gacatcggca acggccgcaa
     1321 gcggcagtgc ggacaatacg agggcggtaa gtttttttcg catatcggct tccttttgta
     1381 aatttgataa aaacctaaaa acatcgggca aacacccgat acgtcttcaa ttataccccc
     1441 ccccccgcaa aaaaccattt ttcagaacaa atatctgata aatgccgcaa cctttatttt
     1501 aaaaatgatt atattttgat ataaaacaat agcttatttt ttcaaaaacg ttgtgtttct
     1561 acaacacaat tcaagcgcag acctcgtgcg agccgatgcg ctgctgcccg gatgcagtct
     1621 cggcttttta aaacgccata aaaaaacaca cgcggcactt tatagtggat taacaaaaac
     1681 cagtacggcg ttgcctcacc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
     1741 aagtgaatcg attccgtact atctgtactg tctgcggctt cgccgtcttg tcctgatttt
     1801 tgttaatcca ctataa
//
LOCUS       DAXHMX010000227         1737 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXHMX010000227 DAXHMX010000000
VERSION     DAXHMX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.227"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            97..1470
                     /locus_tag="ACFM4S_002090"
     CDS             97..1470
                     /locus_tag="ACFM4S_002090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6051693.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1650..>1737
                     /locus_tag="ACFM4S_002091"
     CDS             1650..>1737
                     /locus_tag="ACFM4S_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFC6051694.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYS"
BASE COUNT          443 a          565 c          411 g          318 t
ORIGIN      
        1 gccgccttgt cctgattttt gttaatccac tatatttccc cgtcctatcg gtttcccgtt
       61 tcagacgaca taaggtctga aagaaagact acaattatga gtaatccatt ttcctcttta
      121 ggtttgggta cggaactcgt ttccgcactg accgcgcaag gttacgaaaa cccgacgccc
      181 atccaagccg ccgccattcc caaagcactc gccggtcatg atttgctagc cgccgcgcaa
      241 accggcacag gcaaaaccgc cgcctttatg ctgcccagtc tggaacgcct caaacgttac
      301 gccaccgcca gcacctcgcc cgcgatgcac cccgtgcgta tgctcgtcct tacccccacg
      361 cgcgaacttg ccgaccaaat cgaccaaaac gtgcagtcct acatcaaaaa cctgccgctt
      421 cgccacaccg tcttgttcgg cggtatgaat atggacaaac agaccgccga tttgcgtgcc
      481 ggctgcgaaa tcgtcgtcgc caccgtcgga cggctgctcg accacgtcaa acaaaaaaac
      541 atcagtttga acaaagtcga aatcgtcgtt ctggacgaag cagaccgcat gttggatatg
      601 gggtttatcg acgacatccg caaaatcatg cagatgctgc ccaaacaacg ccaaaccctg
      661 ctcttttccg ctaccttctc cgcaccgata cgcaaactgg cgcaagactt catgaacgcg
      721 cccgaaaccg tcgaagtcgc cgcgcaaaac accaccaacg ccaacgtcga acaacacatc
      781 atcgccgtcg ataccattca gaagcgcaac ctgctcgaac ggctgattgt cgatttgcat
      841 atgaaccagg tcatcgtgtt ctgcaaaacc aaacaaagcg tcgaccgcgt aacgcgcgaa
      901 ctggtgcgcc gcaacctgtc cgcacaggcg atacacggcg accgttccca acaaagccgg
      961 ctcgaaacac tcaacgcctt caaagacggc agcctgcgcg tcctcgtcgc caccgacatc
     1021 gccgcgcgcg ggctggacat tgccgaactg cccttcgtca tcaattacga aatgcccgcc
     1081 cagcccgaag actacgtcca ccgcatcggg cgcacggggc gcgcgggcgc ggacggcgtg
     1141 gcgatttccc tgatggacga atccgaacag aaaatgtttg aatccattaa agagctgacc
     1201 ggcaacaagc tgctcatcga gcgcatcgag ggcttcgagc cgcaatggtg ggaacagggc
     1261 ggcgcaaaac cggaaaaaac cgaaatgcgc gaaccgagac aacgcaaccg ctacgaatcc
     1321 gccagggctc agcgcgaaaa aaatacccga ccggaaacca cggcaaacga tgcgggcgcg
     1381 gcttgcggaa aaattgccgg acgcagccgc cgaagccgtc gggaacaccg gacgtgcgcc
     1441 ctgctccaac cgcgttacgg cgtaaaataa ccctgaaaat caaatgccgt ctgaacattt
     1501 cccgtttcag acggcatttt tcaaaccgga ctgacgcatt gggagcaacc gcccgcaccg
     1561 gataaatttc ttccgcaaac agtttcagac ggcatttgct gcctgtacaa tatcatttcc
     1621 ttttccactc aaaccatcag gatttcccaa tgtccaccca attcgatgtc gccgtaatcg
     1681 gcgcaggtcc ggcaggctcg gtcgcatccg ccctgctccg caaaaaaggt tatagtg
//
LOCUS       DAXHMX010000228         1718 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXHMX010000228 DAXHMX010000000
VERSION     DAXHMX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.228"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            342..1646
                     /locus_tag="ACFM4S_002092"
     CDS             342..1646
                     /locus_tag="ACFM4S_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6051695.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGVVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          351 a          431 c          509 g          427 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcc ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttagtcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccggca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctaccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatcggcg cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acggggtcgt tatggcggca tacagcgggc taatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatggata
//
LOCUS       DAXHMX010000229         1516 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXHMX010000229 DAXHMX010000000
VERSION     DAXHMX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.229"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            184..396
                     /locus_tag="ACFM4S_002093"
     CDS             184..396
                     /locus_tag="ACFM4S_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HFC6051696.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            580..848
                     /locus_tag="ACFM4S_002094"
                     /pseudo
     CDS             580..848
                     /locus_tag="ACFM4S_002094"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(938..1387)
                     /locus_tag="ACFM4S_002095"
     CDS             complement(938..1387)
                     /locus_tag="ACFM4S_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6051697.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          477 a          366 c          233 g          440 t
ORIGIN      
        1 ttttgcaaag gtctcagtct ttttaaacca cagcccttcc caactaaacc aaaaggtcgt
       61 ctgaacccta ttttcagacg accttttgcc actttgtaaa acaaatcttc ccaccatcct
      121 ctccccaaac atcgcccgaa ccagtaaact tctcatattt caacaacttc ttggaagcaa
      181 accatgcccc atatccaccc gctccacctg ttcccgcccc atatcgccga acgcggactg
      241 ctctattttc agcagggcaa agttggtgct gtccaaaaaa cttccaccgg gcgttatcgg
      301 gcggaggtat gcggtttgga aaactattgg gtatggctga agctggatag tgatttgtat
      361 attaaagacg aaagctgcga ttgcccttat gtctaaaggt gcaaacatac cttaaattac
      421 tatattacat aggcaaaata caagcctata acgaattgga aacaaaatgc cgtctgaaag
      481 cattataaaa tctgttcacc ttttcagatg agtaatgtac acccctatac aatttttgct
      541 actatacccc ataaatccac ggctaaagat atccttatta tgtcctatga tttatcgaaa
      601 cgacttgtaa tcggcttagc atcaagtgcc ctattcgact tatccgaatc ggataatata
      661 tttagaatgg aaggggcaga aacctatagg caatatcaga gagaaaaaca aaaccatccc
      721 ctaaaaaagg cgttgtcttt ccatttatta aaaaacttct gtcaatcaat gaaataaacc
      781 caaacgaccc aacaattggg tttattcttt tatccagaaa caatccagat acagattacg
      841 agtcataact ataggcttaa tattacacga ttctcattcc atcaaggcgg aaaaccgcac
      901 aaatactgaa acactatcga tcgatttgta aacaagccta cttaagtaac ttgcagtcct
      961 tatcatttcc tttaaaataa tccagcccgt cactacacga actggcggac ttcttgcaaa
     1021 taaaggttac tagattttca ttcatcttaa taataaaagg atttttatct ttatctatgg
     1081 ctaccgcctt caacatgaat ttactgtcta aggccccgcg cgcgattcca ttcaaacgga
     1141 tacaaaagcc ttctgcctct ttaatcggca aacttggcca tttggtagat gtttgtttaa
     1201 atctcccatt ctgcagataa aacttttcca taaaatgtgc attttctaac aaggctgccc
     1261 gcactgcatt tatctttgct ttctcaacat aattgcgata gctcggataa acaattaaag
     1321 caagtacaga caatatcaag accactgata ttaattcaac cagcgtaaac ccccgattat
     1381 cagtcattac tttacttcca ataagaacag attattcaac atatttcttt gaacagactt
     1441 actatcccat tcagcagtat gcatatttcc cactctattt tttatccgcg ggcagcaccg
     1501 gtttggctgg gccttt
//
LOCUS       DAXHMX010000230         1501 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXHMX010000230 DAXHMX010000000
VERSION     DAXHMX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.230"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            34..1428
                     /locus_tag="ACFM4S_002096"
     CDS             34..1428
                     /locus_tag="ACFM4S_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6051698.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVFPSIRYEADSATDFTGIPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIPKKAQMLQATPSNPTAAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWNSNM
                     LAPLGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          398 a          495 c          342 g          266 t
ORIGIN      
        1 ttatccaccg tccgcctttt tacggaaacc gaaatgaccc cttccgcact gaaaaaaacc
       61 gtcctgctgc tcggcactgc ctttgccgcc gcatccgcac aagcctccgg ctaccacttc
      121 ggcacacagt cggtcaacgc gcaaagcacg gcgaatgccg ccgccgcaga agccgccgac
      181 gcatcgacca tcttctacaa ccccgccggc ctgaccaaac tcgacagcag ccagatttcc
      241 gtcaacgcca atatcgtatt tccaagcatc cgttatgaag cggattccgc caccgacttt
      301 accggcattc ccgtccaagg ttcgaaaagc ggcaaaatca ccaaaaccac ggttgcgccc
      361 cacatctacg gcgcatacaa agtcaacgac aatctgaccg taggcttggg cgtgtacgtc
      421 cccttcggct ctgccaccga atacgaaaaa gattccgtat tgcgccacaa catcaacaaa
      481 ctcggtctga ccagcatcgc cgtcgaaccc gtcgccgcgt ggaaactcaa cgaacgccat
      541 tccttcggcg caggcatcat cgcccaacat acttctgccg agctgcgcaa atatgccgac
      601 tgggggatcc caaaaaaagc gcaaatgctg caagcaacac cttctaatcc tactgccgct
      661 gctcaaatca aagccgacgg acacgccgat gtcaaaggca gcgattgggg cttcggctac
      721 caactggcgt ggatgtggga catcaacgac cgcgcacgcg tgggcgtgaa ctaccgttcc
      781 aaagtctcac acacgctcaa aggcgatgcc gaatgggcgg cagacgacgc aatggcgaaa
      841 caactgtgga atagcaatat gctcgcaccg ctcggctaca cggcgaatga aaaagcccgc
      901 gttaaaatcg ttacgcctga gtctttgtcc gtacacggta tgtacaaagt gtccgataaa
      961 gccgacctgt tcggcgacgt aacttggacg cgccacagcc gcttcgataa ggcggaactg
     1021 gtttttgaaa aagaaaaaac catcgtcaac ggcaaatccg accgcaccac catcaccccc
     1081 aactggcgca acacctacaa agtcggcttc ggcggttctt atcaaatcag cgaaccgctg
     1141 caactgcgcg ccggcatcgc ttttgacaaa tcgcccgtcc gcaacgccga ctaccgcatg
     1201 aacagcctgc ccgacggcaa ccgcatctgg ttctccgccg gcatgaaata ccatatcggc
     1261 aaaaaccacg tcgtcgatgc cgcctacacc cacatccaca tcaacgacac cagctaccgc
     1321 acggcgaagg caagcggcaa cgatgtggac agcaaaggcg cgtcttccgc acgtttcaaa
     1381 aaccacgccg acatcatcgg cctgcaatac acctacaaat tcaaataaac ggcccgccgt
     1441 ttgattgtag aaatgccgtc tgaaacagat ttccgtttca gacggcattg ttttaggctc
     1501 g
//
LOCUS       DAXHMX010000231         1498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXHMX010000231 DAXHMX010000000
VERSION     DAXHMX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.231"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..372
                     /locus_tag="ACFM4S_002097"
     CDS             <1..372
                     /locus_tag="ACFM4S_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6051699.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQTFLDAAELMQWSRETEWLGL
                     NVIAHRNFGKQHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLKPVA"
     gene            complement(663..1457)
                     /locus_tag="ACFM4S_002098"
     CDS             complement(663..1457)
                     /locus_tag="ACFM4S_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6051700.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          393 a          459 c          322 g          324 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcacaac aaacattcct cgatgccgcc gaactgatgc agtggagcag agaaaccgaa
      121 tggctgggct tgaatgtcat cgcacaccgc aacttcggca aacagcacgc ccaagtcgaa
      181 tttgaagcct acttccgaga cggacaacac cgatctgcgc atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtattttatc gatcccaccg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc gggcaaaaaa ttcaaagcct gctgcggcaa atatctgaaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat ttttaacgcg caaaaaaaac
      421 cattcatacc aagggtaagt atgaatggtc aatacattgc gggaaaacgt cttacttgct
      481 gcactgccga aaagggagaa acggcagcgg taatcagcgg aaaggattgt acccgtataa
      541 atgttaagta acgttaatta tgaaaatata accgatgttt cggcacacaa tccgcaactg
      601 tccggcgcag gcggcggcaa agcctgttta ttcaaaccct tattcctgcc catctgtcca
      661 ccttactggt tttcccgtat ccacccgacc cagcgttcaa acagcttcgg ttcgagcgcg
      721 gcaacgaccg agcgtttgaa cacgtgttca ccactccaaa acccgtcgcc ttccaaagtc
      781 gtcagcctgc cgcccgcctc ctcgaaaatc aacgcacccg cagcatagtc ccacagcttc
      841 tgcccaccgt gaacataaac atcataacgc ccgcacgcca gataacacca atccaacgta
      901 ctgctgccca tactccgtat cgttccaaaa ggcgcgagcg tactcatacg gctggaaagt
      961 ttgcccgaac gcaaatattt gatttccacg cccgcaatcg cctcattgag ttttttatcc
     1021 acgagacgca aaggcaggcg ggttccatta agaaacgcac cctgcccccg ttcggcataa
     1081 aaacattcgc cgctgaccgg attgtaaatc acacccaatt cggcgcgccc gttgcggaca
     1141 aacgccaccg acacggcaaa atgcggcagc ccgttgacaa aattgttcgt cccgtctatc
     1201 ggatcgacaa tccacagccc cttttccccc gaatattgtt cccacaaagc cgactgttcc
     1261 tgccgcgaca tttcctcgcc caacatcgga ctgtcgatta aaagcggcaa cgcggcggca
     1321 aaagccgtct gcgcggcaat gtccgcctcg ctcaacatcg aaccgtcttc cttgcggtga
     1381 gacggcgtat tcaaaaaacg cggcataatt tcggtttgcg cgatatggcg cacgactttc
     1441 tgcaaacggt gtaacacttc ctactgtcct catattttga acttgcggca cgcgaacg
//
LOCUS       DAXHMX010000232         1485 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXHMX010000232 DAXHMX010000000
VERSION     DAXHMX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.232"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..519
                     /locus_tag="ACFM4S_002099"
     CDS             <1..519
                     /locus_tag="ACFM4S_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051701.1"
                     /translation="PVGALSKAKAAKQAAPKETINNLANLAKAEQQILFRIAQRDTQL
                     DAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALY
                     RQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPS
                     FIKGTKLELKFQ"
     gene            538..912
                     /locus_tag="ACFM4S_002100"
     CDS             538..912
                     /locus_tag="ACFM4S_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6051702.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            1271..>1485
                     /locus_tag="ACFM4S_002101"
     CDS             1271..>1485
                     /locus_tag="ACFM4S_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6051703.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKT"
BASE COUNT          481 a          300 c          313 g          391 t
ORIGIN      
        1 cctgtggggg cgttgtcgaa agcgaaggcg gccaagcaag ctgctcctaa agaaacaata
       61 aacaatttgg caaatttagc caaagcagaa cagcagattt tattccgtat tgcccaacgc
      121 gatacgcaac tggatgcatg gaagacggga tttaacaata gagtaaggaa aggagcaggc
      181 ttgcttgatg caagtaatat tccgataacc attaacggaa aaaccatcaa acctgtacaa
      241 gccataagct taaagggagc acccgtttac agcggcgtaa gcgaacagga gatttttgcg
      301 ctttatcggc agatgactgg ccagaatccg aattttagag ttttgcctga cggaagatta
      361 gcaaatggca ttatcagtac tggagaatgg gcaggaacaa aaattgcatt aagaaatttt
      421 tcaaaaacag agaattcaac tcaagcacga tggacattag atttgcagaa tcctccatca
      481 tttattaaag gtactaaatt ggagcttaaa ttccaataat ttacaaagga ttttaccgtg
      541 gatgagaaac aaaaaattaa gattcttgat tttcaaatcg atttatcctc aatttttaac
      601 tcttataaaa atcaaatggg tattaatatt caagataaaa acttaaaaaa caatttctgt
      661 tcttttatgg aagaactgtt aaatgacggt tcaatccgtt tacatgatta taccgacggt
      721 atcggaattc ctctaactgg aacttcaaaa gaacaagtgc agaaattgaa agacatatgg
      781 cctactttgg aagatgccca agcaatatgg cctgaagacc cttggtatta cttagaatgg
      841 ctttggtggg atattgcgtg tccaatagat ttggccgatt tgccgaatat tgatatttat
      901 gagcaagcgt aggtatggtt agccgccttt agcggcgtaa ccgtacgcat atcagcaaac
      961 tttataaaat aacaaggccg tctgaaatct gtttttcaac tttttcagac ggccttgcaa
     1021 cttggcattt cattcgtacg gttacgccgc taaaggcggc taaccgtacc tacgagctct
     1081 gataaaaatg atttatggaa gcaagctgta gcctgcatga aacctaaaat ccatgcgtaa
     1141 ggtgtgtgct tcagcgcgca cgcgttccat gatttacggc tcaatgccgt ctgaaaagct
     1201 caattttttc agacggcatt tgttatgcaa gtaaatattc agattcccta tatactgccc
     1261 agacgcgtgc gtgctgaaga cacctcctac gcttgctgca gaactttcgg gtaaaaccgg
     1321 tgtgagcatt agcgcaccgt atgccaatga gaacagtcgc atcctgctca gcaccacgga
     1381 tatcagttcg gaaaacggca aaatcaaaat tcaatcttac ggtgaccaat attactatgc
     1441 gagacagagc gaactctata cctttgaacg ccgcagctac aaaac
//
LOCUS       DAXHMX010000233         1458 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXHMX010000233 DAXHMX010000000
VERSION     DAXHMX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.233"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..217)
                     /locus_tag="ACFM4S_002102"
     CDS             complement(<1..217)
                     /locus_tag="ACFM4S_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC6051704.1"
                     /translation="MTYANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWK"
     gene            1252..1428
                     /locus_tag="ACFM4S_002103"
                     /pseudo
     CDS             1252..1428
                     /locus_tag="ACFM4S_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin B"
BASE COUNT          498 a          270 c          202 g          488 t
ORIGIN      
        1 tcttccaatt actgcttaaa aagctatctg cagcttgaat agcattatta acagtaattt
       61 ccattttatt tctattgtta tccacattat agattaaatc agataaggat tgaatagaac
      121 caataaatga atcccaagct ttatatccca agagtggtga atagggtgtt aatttcacat
      181 ctccaaaacc gttaatatta aaatttgcat atgtcatatc tgctttcctt tcaagtaaat
      241 taaaaaaata acgtgaggaa ttaaaattaa aaataaatta attatcgact caatataata
      301 tgaatcaatt agctctctta tggtaaatat aagaggtaaa aatactaaaa ataaactaat
      361 aaagttcaga aaaatagtaa atggaatatt ttcatatgga aaaattctac aaaatacaag
      421 taatattcca tgacatatta gcaaatgagt aaaatttatt ataaaatata taaatatttc
      481 cccatcgtaa gatatattca aataaagaaa tataaacaat atgaagtata taaaatatat
      541 tttttggcat ctaaaaaata aacttttagc aatcatatat aaatagcttg tagaataaaa
      601 aacgaaaatt attaattatc gtaggtgtgc atttattcca acacggtttc atgcagctta
      661 aaaaccaaaa attctttctc cctttatcct ttcttaaaaa tagcgttttc aaattctgca
      721 acgacaggtt tcattcaagt aggactgccg tgattgcatc aattgcttcg cggcatggca
      781 tccatatcac acaacaggac atttattctc gggtaagtcc accttcatcc tcttccgcca
      841 gcaggaacag ttacaggcta cctaaaatat gctctgtgct cggatagtaa atatttcctt
      901 tctcactatt gtttgtcatt taatcgacag tctactcttt ctctaaaatt tcgtcaataa
      961 tagttaaata tatttaaata catttaaaat aataatatac acctgtttca gtctgtctaa
     1021 aacacgggaa aatatgttat ttgatcacaa cggtgacttt gggcagcctg taccttcaat
     1081 aacgcgtgga aaatagatat atacttatat cgtattcgag gctgtcttgt ttccctcaaa
     1141 caaccattct tccacacttt tgctttacaa ttttcgtgtg tatccttaca attaccccta
     1201 ttgtttttgt atattccttt gccaaccgtt attgctatcc aatctaatat tatggctatc
     1261 gtatctacac cacaccccgc tctttccgcc ctcatcatcc tcgcccatta tcacggcatt
     1321 gccgccaaat ctttgggatt gaaggcaaag gtagtacgcc agcctgtcaa acgtttggct
     1381 atggtgacct tacctgcatt ggtatggtgt gatgacggca accattttaa ggaaaagtgg
     1441 ttgttttcgg cattctga
//
LOCUS       DAXHMX010000234         1441 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXHMX010000234 DAXHMX010000000
VERSION     DAXHMX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.234"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(80..1417)
                     /locus_tag="ACFM4S_002104"
     CDS             complement(80..1417)
                     /locus_tag="ACFM4S_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6051705.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGSGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLGIL"
BASE COUNT          448 a          381 c          350 g          262 t
ORIGIN      
        1 tgaacgaaat aggctgaacc tgaagcaaaa atgccgtctg aaaggctttc agacggcatt
       61 tttgccgttt ggagggcttt taaaggatgc ccaaagaatt gatgaaaaca ataataatgg
      121 caatcggggc aaggtagcgc aaggtattca gccacagcag cagcactgcc cgtggtacgg
      181 tgctgcccga tccggcatct tttaacacag actgcttgtc ctgaatccag gcggtaaaga
      241 tggaaacact caaagcacca atcggcataa tgacggcgga aataacatag tcccacaaat
      301 caaaaatggt tttgccgaaa accttaaact caccccatac gccgaaagac agcgcggacg
      361 ggatgccgac aatgaaaata gccgtgccga taagccaagt gtgctttttg cgtttgcgct
      421 cgtcttggcg gatggttgag gcaatgaccg tttccaacat cgaaaatgcg gaagtcagcg
      481 tggcgaaaac gaccagaagc atgaataccg caaacaaaac cgtaccgaac ggcatcttca
      541 taaacactgc gggcaatacg ataaaaatca atcccggccc ctggctcggt tcaaaaccga
      601 aggcgaacac cgccggaaaa atcaccaggc cggcaagcag cgaaaccaag aggttcatcc
      661 acataatcgt atggccggaa cggaacatat cctgatcttt tcccaaatat gaagcgtagg
      721 taatcatggc ggaaacgccg atgctcaggg caaaaaacgc ctgtcccaaa gccgtaatca
      781 tcgtatcggc tttaaagtac gaccaattcg gtttgagcag gaaagacacg ccctccattg
      841 cacccggcag cgtcagcgaa cggattgcca gcgcaataaa gaggataaac agccccggca
      901 tcagataacg gtttgccttt tcaatgccgt ctgaaatccc gcctttgacc acccaaaccg
      961 taatcagcat aaacagtgcc tgataggaca gcgaacctgc cggattggaa atcgttgtgc
     1021 cgaacagggc ttcaaagtcc gctcctgcat ggattgcccc tgtaaaactg tggacgacat
     1081 aattcaatac ccatccgccg accacgctgt aaaacgacag caaaataaag caggcggcaa
     1141 cgcccatgcg cccgacccaa agccattgcg tgcccggacg cagagccctg aaggaatcga
     1201 cggcattttt accgcccgtg cgcccgatat aaaactcggc aagctggacg ggcagggcga
     1261 ccaagatagt aaatatcaaa aacagcagga aaaacaccgc gccgccgttg gtgcctgccg
     1321 tataagggaa tttccaaatc gcgcccaaac cgatggccga acctgccgca gcaaggacga
     1381 aaccgatttt ggacgaccaa gaagtatggt tactcataaa atttattaag aaatgtgaaa
     1441 g
//
LOCUS       DAXHMX010000235         1434 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXHMX010000235 DAXHMX010000000
VERSION     DAXHMX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.235"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            128..925
                     /locus_tag="ACFM4S_002105"
     CDS             128..925
                     /locus_tag="ACFM4S_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6051706.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGAIWNGLPPAILSVSPVL"
     gene            922..1257
                     /locus_tag="ACFM4S_002106"
     CDS             922..1257
                     /locus_tag="ACFM4S_002106"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6051707.1"
                     /translation="MNNVSEVIKEHHIFVLPSYYREGVPRSTQEAMAVGRAVITTDVP
                     GYRETVADKVNGFLIEPWNPRILAEKMIYFIENREAVRLMGNASYAIAKDKFDAEKVD
                     LKLLDILKA"
BASE COUNT          387 a          343 c          338 g          366 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgataat gaattggata ttatcaggga aatgggggtt acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttccga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 cttgattgcc ttataccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt cgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaattcc tctttatcgg cagatttctg aaagaaaagg ggattgatga
      781 ttttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcgggggggg ggcgatttgg aacggcttgc cgcccgcgat
      901 attatccgtt tccccggttt tgtgaacaat gtttccgaag tgataaaaga acatcatata
      961 ttcgtattgc cgtcttatta tagggaaggc gttccccgaa gcactcagga ggcaatggcc
     1021 gtcggcaggg cagtgattac gacggatgtc cccggataca gggaaacggt cgccgacaag
     1081 gtcaacggct tcctgatcga accttggaat ccccgcatct tggccgaaaa aatgatttat
     1141 tttatcgaaa acagggaagc cgtccgcctg atggggaatg caagttatgc gattgccaaa
     1201 gataaattcg atgccgaaaa agtcgatttg aaattgctcg atattttgaa ggcgtaaaca
     1261 aggctgcccg cttttgagtt tcggtcattt ctgataaacc cccgtcattc ccgccacacc
     1321 cccgtcattc ccgcgaaagc gggaatccag gttcgttgag tttcggtcat ttccgataaa
     1381 ttcttgcaac tttgagtttc tagattccca ctttcgtggg aatgacggcg gagg
//
LOCUS       DAXHMX010000236         1406 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXHMX010000236 DAXHMX010000000
VERSION     DAXHMX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.236"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..468
                     /locus_tag="ACFM4S_002107"
     CDS             <1..468
                     /locus_tag="ACFM4S_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6051708.1"
                     /translation="PVGALSKAKAAKQAIPTQTVKELDGLLQESKNIGAVNTRINIAN
                     STTRYTPMRQTGQPVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSP
                     VSMTPDGQYMRTVDVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            475..984
                     /locus_tag="ACFM4S_002108"
     CDS             475..984
                     /locus_tag="ACFM4S_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051709.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            1053..>1406
                     /locus_tag="ACFM4S_002109"
     CDS             1053..>1406
                     /locus_tag="ACFM4S_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6051710.1"
                     /translation="MIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLFEAP
                     RLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVNWNQ
                     VQLAYDKWDYKQEGLT"
BASE COUNT          476 a          271 c          261 g          398 t
ORIGIN      
        1 ccagtaggag cgttgtcgaa agcgaaggcg gctaagcagg ctataccaac ccagacagtt
       61 aaagaacttg atggcttact acaagaatca aaaaatatag gtgctgtaaa tacacgaatt
      121 aatatagcga atagtactac tcgatataca ccaatgagac aaacgggaca accggtatct
      181 gctggctttg agcatgttct tgaggggcac ttccataggc ctattgcgaa taaccgttca
      241 gtttttacca tctccccaaa tgaattgaag gttatacttc aaagtaataa agtagtttct
      301 tctcccgtat cgatgactcc tgatggccaa tatatgcgga ctgtcgatgt aggaaaagtt
      361 attggtacta cttctattaa agaaggtgga caacccacaa ctacaattaa agtatttaca
      421 gataagtcag gaaatttgat tactacatac ccagtaaaag gaaactaact aaatatgagt
      481 aactttgaaa aaaaatatat tttagaatta aatgatgctt taagccattt aaatcataac
      541 tctacctcat ttgatttatt gaaagttttg atttcatggt tatcaaacga tattgtcatt
      601 gataaattta aaattttagg ttatgacttt agtaaatata tcgaaatgaa tcccgatgac
      661 tatccggttg aaaaatctat attgaataga gaggaaatta tttatctcaa aaacaatatt
      721 tatcgtaaaa tatcatcagg aaattttaaa tttcaatact ttgtacaata tattagagat
      781 attttagaat atttatttat tgaacatatt gaaagagtct gtccttactg cgaatggggt
      841 gaaatgcaaa aattagaaga acaaaatacg catgaaacgg tgtatctctg tactcaatgt
      901 ggatgtgctt tttataacga taattcacaa tttttattaa aaaccccttt aaccattcca
      961 atgaaacgtg atgaatttaa ataaacaagc cgtagcctgc atgaacccta aaatccacgt
     1021 gtagcgtgtg tgcgccagca cgcatgcgtt ccatgattta cggctcaatg ccgtctgaaa
     1081 agctcacaat ttttcagacg gcatttgtta tgcaagtaaa tattcagatt ccctgtatgc
     1141 tgtacagacg cgggagtgtt aagcccccct tgtttgaagc tccgcggctc ctgccgagct
     1201 tcaccgaccc cgttgtgccc aagctctctg ctcccggcgg ctacattgtc gacatcccca
     1261 aaggcaatct gaaaaccgaa atcgaaaagc tggccaaaca gcccgagtat gcctatctga
     1321 aacagctcca agtagcgaaa aacgtcaact ggaaccaggt gcaactggct tacgataaat
     1381 gggactataa gcaggaaggc ttaacc
//
LOCUS       DAXHMX010000237         1403 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXHMX010000237 DAXHMX010000000
VERSION     DAXHMX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.237"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(41..1033)
                     /gene="porB"
                     /locus_tag="ACFM4S_002110"
     CDS             complement(41..1033)
                     /gene="porB"
                     /locus_tag="ACFM4S_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6051711.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALHASVAVQQQDAKLVEDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFD
                     DADLSNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVSTAGGVGLRHKF"
BASE COUNT          319 a          323 c          366 g          395 t
ORIGIN      
        1 gtctggcgac aggctttttg ttgataccaa tctttgcaga ttagaatttg tggcgcagac
       61 cgacaccgcc ggcagtcgat acgaatttgt tttcgccttt gccttcttgc aaccaaccgg
      121 cagaaaccaa ggcagaagtg cgtttggaga agtcgtattc cgcaccgaca accacttggt
      181 cgtaatcgtt gcttaagtct gcatcatcaa acgagccttt gaagccgtgg gcgtaagaaa
      241 cgcggggcgt tacgttgccg aagcggtatg ccaaggtagc ggcaacttcg gtttgagagt
      301 tgtgcgaata attgtcttca accaatttgg catcttgttg ctgtacggct acggaagcgt
      361 gcagggcatc attgtcgtaa ccgctgacca aacggtgaat ctggtatttt tcaatcttca
      421 cgtcatccac atcctgatgt cttttatagg caccgccata ttgcacgaag aagccgccgt
      481 ttttgtagtt gaagccggcg tggtaagatt cgctgttatg tctgcctgca ttgtcgttaa
      541 gcgcgtattg tacgctgccg ctgaggccgg caaattcggg agaatcgtag cgtacggaaa
      601 tgaggcgtgc ttcgggttcg gcaattttgt ttacacccaa atagtcgctt ttgctatccc
      661 aaggattgat gtcgccggtg tctttcagga cgctgttcaa acggccgacg cgcaatttac
      721 cgaagccgcc tttcaaaccg atgaaggatt ggcggttgcc ccaaccggag tcagtgccgg
      781 cgatagatgc cttttgctca acctgccaaa tggctttcag accgttgccg aggtcttctt
      841 ggcctttgaa gccgattttc gaacccaaat caacgatgcc ggtaccggtt tcaacgctaa
      901 ccacctgacc tccattgtgc tctacagagc gggaagtttc tacgccggct ttgatggtgc
      961 cgtacagggt aacgtcagcc attgctgcaa caggaagggc tgccaaagtc agggcaatca
     1021 gggatttttt cattgctgta ttcctttttt ggttaagaaa tttaagcagg ccgggctttc
     1081 caagccgctt agctttgcat ttaccgccga cgttttcggc gattccatgt ctgaaactat
     1141 agatgttcct gtctgaaaaa acaaacttct gcctgttttt gtgtggaatt cgatgtgtat
     1201 tttgaagggc ggatagagat attgtgggta ttttttgttt tataacatat ggttatttaa
     1261 attttttaag atttgcattt ttacaacact tactcgggag ggtattggag ggcattgcaa
     1321 accgggggtt ataaagacgg caaaaaacgc gcacgtggtt tctttcggaa cgggcggatg
     1381 caaaacccgc ccctgcggca gga
//
LOCUS       DAXHMX010000238         1350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXHMX010000238 DAXHMX010000000
VERSION     DAXHMX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.238"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..704
                     /locus_tag="ACFM4S_002111"
     rRNA            <1..704
                     /locus_tag="ACFM4S_002111"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            801..914
                     /gene="rrf"
                     /locus_tag="ACFM4S_002112"
     rRNA            801..914
                     /gene="rrf"
                     /locus_tag="ACFM4S_002112"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            950..>1350
                     /locus_tag="ACFM4S_002113"
     CDS             950..>1350
                     /locus_tag="ACFM4S_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFC6051712.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQA"
BASE COUNT          322 a          305 c          406 g          317 t
ORIGIN      
        1 ctagacccgt catccgggtc ggggaccgtg catggtaggc agtttgactg gggcggtctc
       61 ctcccaaagc gtaacggagg agttcgaagg ttacctaggt ccggtcggaa atcggactga
      121 tagtgcaatg gcaaaaggta gcttaactgc gagaccgaca agtcgagcag gtgcgaaagc
      181 aggacatagt gatccggtgg ttctgtatgg aagggccatc gctcaacgga taaaaggtac
      241 tccggggata acaggctgat tccgcccaag agttcatatc gacggcggag tttggcacct
      301 cgatgtcggc tcatcacatc ctggggctgt agtcggtccc aagggtatgg ctgttcgcca
      361 tttaaagtgg tacgtgagct gggtttaaaa cgtcgtgaga cagtttggtc cctatctgca
      421 gtgggcgttg gaagtttgac gggggctgct cctagtacga gaggaccgga gtggacgaac
      481 ctctggtgta ccggttgtaa cgccagttgc atagccgggt agctaagttc ggaagagata
      541 agcgctgaaa gcatctaagc gcgaaactcg cctgaagatg agacttccct tgcggtttaa
      601 ccgcactaaa gagtcgttcg agaccaggac gttgataggt ggggtgtgga agcgcggtaa
      661 cgcgtgaagc taacccatac taattgctcg tgaggcttga ctctatcatt tgaagaactt
      721 caagagataa aagcttactg actgattcag tcattaccga atatattgat taaggcttta
      781 ccgatttgta acagtttaag tttggcggcc atagcgagtt ggtcccacgc cttcccatcc
      841 cgaacaggac cgtgaaacga ctcagcgccg atgatagtgt ggttcttcca tgcgaaagta
      901 ggtcactgcc aaacacccat tcagaaaacc cccgattatt cgggggtttt tgctttgccc
      961 ggaaaaaatg tcggggatgg cgggacggca tccgtacggc gtccggtcgg gtttgcggag
     1021 gaacggcttg aaactttggg atattcattt tagaatgact cgttttatcg tcgcaagatg
     1081 cggtttattg tttgcaaccc ttaaaggaaa aaccatgaag aaaatgttcg tgctgttctg
     1141 tatgctgttc tcctgcgcct tctcccttgc ggcggtaaac atcaatgcgg cttcgctgca
     1201 ggagttggag gcgctgccag gcataggccc ggcgaaggcg aaggccattg cggaataccg
     1261 tgcgcaaaac ggtgcgttca agtctgtaga cgatttgacc aaggtaaagg gcatcggccc
     1321 tgcggtgctg gcgaagctga aggatcaggc
//
LOCUS       DAXHMX010000239         1341 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXHMX010000239 DAXHMX010000000
VERSION     DAXHMX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.239"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            1106..>1341
                     /locus_tag="ACFM4S_002114"
     CDS             1106..>1341
                     /locus_tag="ACFM4S_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6051713.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGEWPGDNSSAGVATSAD"
BASE COUNT          432 a          380 c          246 g          283 t
ORIGIN      
        1 agcgcaccgc ccccaccacc accaacgacg acgtcaaagc cgacaccgcc gccaacggca
       61 agcagatcga caccaagcac ctgccgtcaa cagcatcgac aagaaaatcg acaccaaact
      121 agagaaacct ttttacccga taagtttccg taccgacggg gctggattcc cgcctgcgcg
      181 ggaatgacag cggagcggtt tctgtttttt ccgataaatt cctaaaactt aaattttcat
      241 cattcccaca aagacagaaa accaaaaaca gaaacctaaa attcggcatt cccgcaaaag
      301 cgggaatcta gttcgttgag tttcagttat ttagataaat tttgaaactt taatcccgtc
      361 attcccacga aagtgggaat ccaggacgca aaatctcaag aaaccgtttt cccgataagt
      421 ttccgtgccg acgaggctgg attcccgcct gcgcgggaat gacgaagcga tccatacgga
      481 aacctgcacc gcgtcattcc cacgaaagtg ggaatccagg acgcaaaatc tcaagaaacc
      541 gttttcccga taagtttccg taccgacaga cctagattcc cgcctgcgcg ggaatgacga
      601 agccatccgc acggaaacct gccgcgatga ttcaactgcc gtttgcacag gaacaccacg
      661 cgccgatttc aaacactttc caagaaaacg gaaattttta aaaattaaat ttaaaatttc
      721 ccacccaacc caccctatgc taccgcgcaa attcaaaaat ctcaaattcc gacccaatca
      781 acacacccga tgccccatgc caatagagat accccacgcc aataaaaaag tgccgaaaat
      841 cggcactaaa actgacaatt ttcaacactg ctgacaaaaa acggtaatcc cctgcttcct
      901 caaaccacac ccaaacgaaa gaaaatgtaa aataaaaaca accatataaa gataaacgca
      961 taaattttca cctcaaaaca caaacttggt accaatcttg ctttataata cacaggtatc
     1021 gcaaaaaaat accgatggtc aaatacattg cataatgccg atggcgtaag cttgaagcat
     1081 ttcctttcca attaggagta attttatgaa cacccttcaa aaaggtttta cccttatcga
     1141 gctgatgatt gtgattgcca tcgtcggcat tttggcggca gtcgcccttc ctgcttatca
     1201 agactacaca gcccgcgcac aagtttccga agccatcctt ttggctgaag gtcaaaaatc
     1261 agccgttacc gagtattacc tgaatcacgg cgaatggccc ggcgacaaca gttctgccgg
     1321 cgtggcaacc tccgccgaca t
//
LOCUS       DAXHMX010000240         1299 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXHMX010000240 DAXHMX010000000
VERSION     DAXHMX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.240"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..378)
                     /locus_tag="ACFM4S_002115"
                     /pseudo
     CDS             complement(1..378)
                     /locus_tag="ACFM4S_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(562..984)
                     /locus_tag="ACFM4S_002116"
                     /pseudo
     CDS             complement(562..984)
                     /locus_tag="ACFM4S_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          254 a          291 c          368 g          386 t
ORIGIN      
        1 agcgtcgcgg caggttgacg gcaggtgctt ggtgtcgatg ttggcggtgc ccttgccgtt
       61 ggcggcggtg acttcgtcgt tgttggcgcc gttggcgttg gcgcgcgcaa ccggctgtcc
      121 gcagaaccat tttaccgaac cgtcttgacg cttggcccac agggagagtt ttttgtcttt
      181 gatttcgttg tttacgttgc ttgaagccat tgtggcggta atgacgcctt tttcaacttt
      241 aacccctgca acatatttgc ctttgatgtc ggtggaggat gccacgccgg cagaactgtt
      301 gtcgccgggc cattcgccgt gattcaggta atactcggtg acggctgatt tttgaccttc
      361 agccaaaaga atggattcgt cattcccgcg caggcgggaa tccagcctcg tcggtacgga
      421 aacttatcgg gaaaacggtt tctttagatt ttacgttctg gattcccact ttcgtgggaa
      481 tgacgggatt taagtttcaa aatttattct aaataactga aactcaacga actagattcc
      541 cgcttttgcg ggaatgacgg tttaggtatt tttatagaaa gccgtaggtg gtgtttctat
      601 gcaaacggca gatgctgcgt cgcggcaggt tgacggcagg tgcttggtgt cgatcttctt
      661 ggtggtggcg gtgtcggctt tgacggcgtc ggcgctggcg gtggcgtcgc gcgtaaccgg
      721 ctgtccgcag aaccatttta ccgaaccggc ttgacgcttg gcccacaggg agagtttttt
      781 gcctttgatt tcgttgttta cgttgcttga agccatttgg gcggtaacga cgccgttttt
      841 gacttcaacg cttttaacat atttgccttt gatgtcggcg gaggttgcca cgccggcaga
      901 actgttgttg gcgggccatt cgccgtgatt caggtaatac tcggtaacgg ctgatttttg
      961 accttcagcc aaaaggatcg cttcgtcatt cccgcgcagg cgggaatcca ggtctgtcgg
     1021 tacggaaact tatcgggaaa gcggtttctt gagattttac gttctggatt cccactttcg
     1081 tgggaatgac gggattaaag tttcaaaatt tattctaaat aactgaaact gaacgaacta
     1141 gattcccgct tttgcgggaa tgacgaattt taggtttctg tttttggttt tctgtctttg
     1201 tgggaatgat gaaattttaa gttttaggaa tttatcggaa aaaacagaaa ccgctccgcc
     1261 gtcattcccg cgcaggcggg aatccagccc cgtcggtgc
//
LOCUS       DAXHMX010000241         1277 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXHMX010000241 DAXHMX010000000
VERSION     DAXHMX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.241"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(28..699)
                     /locus_tag="ACFM4S_002117"
     CDS             complement(28..699)
                     /locus_tag="ACFM4S_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6051714.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNTVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMERGRGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          314 a          285 c          295 g          383 t
ORIGIN      
        1 ggggttttgg ggaattttgc aaaggtctca gtctatgccc gatatacaat tttgatacac
       61 aaacttggaa atatcggtat cgtcgccgga gcgatagaat gcggacagtt tttcattgaa
      121 ttgcggcatc tgcatttcgg agatttccaa gatgccacgg cctctctcca tcatcagacc
      181 attggcaaat aaggttgccg ttcgtttgtt gccgtcccaa aaaacctgct gccgcataca
      241 ataaagcata aaacggacgg ctgcctccgt cgtcgaaccg ctttgcagtc cgatattttg
      301 taacacgcgg gccacttcaa tttccttcac tggattcggg gcatgacggg aaccgtccaa
      361 tagcgttacg ccgaccgaac cggtacggaa atcaccgggt gccaaagaat cgtccttggc
      421 aacagtgttg ttgatttttt taaggagtga aatatcgacc ggttcgccgt ttgaaatatg
      481 cgaaatcaca tattgatagg cacgtttcag gttcaagatg gtttggatgt cttccagtga
      541 cgcggaggct acatttttgc catggataat ttgttcggtc tgcaacaagg tcgtctgaca
      601 attctcaaaa cggctgaggt tgtgaatttg ggcaaccaat actttctttg ccaaaaaaat
      661 attttctttc aaactcaact tgtatttgtc gggaaacacg ataattcctt aaataattaa
      721 agcagtacgt taaatcatca tcttatcatc actttcgggg tttatgttgc gtgcgtccgt
      781 tttctaatac ggtttcccat cccgcgaaac gaggcggagg cctgtgcaaa aaacctgttc
      841 acgaaatttt ccgcattaac gatacgctga aaatgcgcta aaaatgactg tgtttgaata
      901 tcggttgatt tcatccgttt gtgtaacctc gcgccggttt tgtcatgatt ttgcattata
      961 gtgaattaaa tttaaaccag tacagcgttg cctcgcctta ccgtactatc tgtactgtct
     1021 gcggcttcgt cgccttgtcc tgatttttgt taattcacta tatttttgtc atgcggatat
     1081 ttcacgggat gacaaaacgg gcgcaaaaaa gcccgattgg aaaatccgaa tcgggctttt
     1141 ttgtgatgtt ctgttcgatt tgcgtactaa ctgccggccg gagcgtccgc gcccggtttg
     1201 gcaggtcgga ttctcgaatc cgacggctat ttgagatggc aggcttttgt cggatacaag
     1261 tatccgacct acggctt
//
LOCUS       DAXHMX010000242         1221 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXHMX010000242 DAXHMX010000000
VERSION     DAXHMX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.242"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(129..1136)
                     /locus_tag="ACFM4S_002118"
     CDS             complement(129..1136)
                     /locus_tag="ACFM4S_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6051715.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPASDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYIPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNMLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGI
                     VWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHN
                     SSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIA
                     F"
BASE COUNT          282 a          329 c          348 g          262 t
ORIGIN      
        1 ccaaacacag tcaaacaaat ttatatgccc cgtcccttct gaatgatttg aaaacacagc
       61 cgccaaaaac aaaaatgccg tctgaaaacc tttcagacgg catttccaat ttgatttcag
      121 gcagaaagtc agaacgcgat atagctgttc gggttaaccg gtttgccgtt ttgccgcacc
      181 tcgaaatgaa gctgcgttct ggaagcgtcg gtattgccca tcaaagcgac ctgctgcccg
      241 cgtttgacct gctggccttc gccgaccagc aatttttggt tgtgcccgta tgcggtcagg
      301 aaggaagaat tatgctggat gatgaccaga ttgccgtatc ccctcaaacc ggaacctgca
      361 taaaccactt tgccgtcagc cgccgccaaa acgggctgtc ccgcattacc ggcaatatcg
      421 acacccttgt tgttgccgcc gaaatcggca accactttac cttgcgtcgg acgctgccaa
      481 acaatgccgc cgaccgaacg cgtgccggaa ggcgaagcgg caggagattg cggggcgggc
      541 gcgggaaccg ctttattttc cgcagcgggc gcggcaggtt gcggcgcgga ctgcacaggc
      601 ggttgcgcgg cgggtttcac aggggtttgc acggcagccg gtacggcggg cctgctttct
      661 acggctgcgg ttttcggtgc ggcatatcct gccggtttga ctttaacaat ctgaccgatg
      721 ctcaacatat tgtcggtcat gccgttccac gcacggaaat cgtcttgaga gatatggtag
      781 cgtttggaaa tgttgtacac cgtgtcgccg cgcacaatag tatgcgtcgc cgcgttgatg
      841 tcgacgggtg cgtaagaagg aatgtatgta cccgaaacgg caggtgcaga cggcggaaca
      901 taagcaggag gcgtataaac cggcgcgctt tgcaccggcg gcacataagg cgcatcgctg
      961 gcaggagccg ggctgtacgg cgttgctcca taggggttgt tgtaaactgc cgaagacggc
     1021 gcgtcctgca tacctgaatt gcctgcaatg acgggggcag gctgctgggt agcacaaccg
     1081 cccaacagtg cggcaaagga ggcgcaagcc gccaaaagtg tcgtttgttt caacataaga
     1141 taaccttcat gttccgatat atagcctgaa tgcggtatat cataataaaa atgcgcgttc
     1201 ttctcaagcg caaagcccga c
//
LOCUS       DAXHMX010000243         1082 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXHMX010000243 DAXHMX010000000
VERSION     DAXHMX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.243"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            284..721
                     /locus_tag="ACFM4S_002119"
     CDS             284..721
                     /locus_tag="ACFM4S_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HFC6051716.1"
                     /translation="MSRYLITFDMDTNCLKDNYHGNNYTNAYSDIKTILARHGFENIQ
                     GSVYLGREGISEAHGTIAIQELTARFDWFYSCISNIKFYRLESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
BASE COUNT          338 a          248 c          219 g          277 t
ORIGIN      
        1 gccaccaaaa cccttgctag ctaagggtta aacagctcac ttgaaatcta cttaagtcta
       61 atctaaacta tccaatatgg atagattttt aaacataggg caagcagcaa aattattgta
      121 gctgaaagca caatcactcg ctggtggtct caaacacgtg ccgactacct cgccgaaaac
      181 actatcagcc gcgataaacc gtgggaaaag ctcgttatca gccgccgcac ttggtactat
      241 cgcgggaaac cgatgctgtc tgaaacgcaa caggagaaaa ataatgagcc gttacctgat
      301 tacctttgat atggatacca actgcctgaa agacaattac cacggaaata actataccaa
      361 tgcctactcc gatattaaaa ccatcttggc tagacatgga tttgagaaca ttcagggcag
      421 tgtttatcta ggccgtgaag gcatcagtga agcacacgga acaatagcca ttcaggaact
      481 gaccgctcgg tttgattggt tttactcctg tatttcaaac attaagtttt accgccttga
      541 aagtgatttg aacgcacaat ttatcgctga tggtgtgtat caagccaaac aggctttcct
      601 tcaacgtgtt gaacaacttc gtatatccct aacagaagct ggattgtctg atgagcaaat
      661 caatcaggtt ctggaaaaac agaaatttga attggaaagt cctaacctga aattaaatta
      721 acctccttta ctcaccaaca tccgccgcag ctctgtcagt ttttggcgcg ctgcggcgat
      781 ttctgtgcgt tttagagctt cgggtagggt gtgaaacaac tcactcgaaa tttacttaag
      841 tctaatctaa actatccaag cagtaattag tacaaaaaag gcaaacttat tttaggagct
      901 taaaattgca gccgcgataa accgtgggag agtctcggca tttcccgcgc cacttggtac
      961 aaacgtggca aaccgatgcc gtctgaaacc gtacaacagg agacaaaata atgcttgctc
     1021 aaatcgaact cacaccctgc cagctaatgg tttacgtaac ttaaggttac tggatttacg
     1081 ca
//
LOCUS       DAXHMX010000244         1049 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXHMX010000244 DAXHMX010000000
VERSION     DAXHMX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.244"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..165
                     /locus_tag="ACFM4S_002120"
     CDS             <1..165
                     /locus_tag="ACFM4S_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6051717.1"
                     /translation="TKQVTKGVFDGKGEAKIPSYTWHHHQDTGRMQLISERKHTQTGH
                     IGGEAMDKGK"
     gene            168..566
                     /locus_tag="ACFM4S_002121"
     CDS             168..566
                     /locus_tag="ACFM4S_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051718.1"
                     /translation="MLGLTRNNCDDLGFAILCYERNAADKKEFLDWLYEIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQEFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            667..>1049
                     /locus_tag="ACFM4S_002122"
     CDS             667..>1049
                     /locus_tag="ACFM4S_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051719.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPP"
BASE COUNT          346 a          170 c          228 g          305 t
ORIGIN      
        1 acaaagcagg taaccaaagg agtgtttgac ggaaaaggag aagctaaaat accgagctat
       61 acttggcatc atcatcagga tacgggacga atgcagttaa tatccgaaag aaagcatacg
      121 caaacagggc atataggtgg tgaagcaatg gataaaggaa aatgattatg ttggggctta
      181 cacggaacaa ttgtgatgat ttgggatttg ctatattgtg ttatgagcgc aatgcagccg
      241 acaaaaaaga attcttggat tggttatatg aaattgtcag gactgtccct gccgaaagtt
      301 ttccaaggta tctgattgat ttaatagatt gtttggaatc agatgattgt tccataaata
      361 tatataatat tattggcttt gttccaacta ccggtatgac gaaaaaggaa aaagatgccg
      421 tatatggcat tacctatata cggaatcaag agttttacga tattccgatt actagggaga
      481 aggcgttaaa agccttaaaa aaacatcctg aaatttatca gaagtttcgg catttctttc
      541 cgtttgtaga actgcccccg ctttaaacag tcaaaatgcc gtctgaaacg atattcggct
      601 ttcagacggc atttttgaca caaagcaggt aaccaaaaga gcgtttgacg gcaaaggaag
      661 ataattatgt ggaaaatcat aaaagaggat agtgatgatt taggatttgc aattaaatgc
      721 ttattctctc aatctattga tttaaatgaa ttcaagttat ggattgaaca agtaatacga
      781 gatatgccca ttgaggacat ccctttttat atttttgact tggcggattt cgatgtagga
      841 attggcgata ttggcaatat tgtaggtttt gctccgagtt ccagcctgcc gaaatcgaaa
      901 aaaaatgcct tgaccggcat tgccttctta agggggatag atgtctatga ccctcccgtt
      961 tcaaaagaaa aagcattaaa agccttagag aaatatcctg aaatttatca gaaatttcag
     1021 catttctttc cgtttgtaga gctgcctcc
//
LOCUS       DAXHMX010000245         1035 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXHMX010000245 DAXHMX010000000
VERSION     DAXHMX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.245"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(302..688)
                     /locus_tag="ACFM4S_002123"
     CDS             complement(302..688)
                     /locus_tag="ACFM4S_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC6051720.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGTSARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(685..930)
                     /locus_tag="ACFM4S_002124"
     CDS             complement(685..930)
                     /locus_tag="ACFM4S_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFC6051721.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMGNTDISRILEDDNETAALFEESAL"
BASE COUNT          281 a          244 c          184 g          326 t
ORIGIN      
        1 acacagccat atatattaag tacctgctct ttatgcgctt ttttaggaaa ggaagatcaa
       61 acggttcaat agcgaagtct tgcccaaatt tgatggacag gatatagaat acatgcgtgc
      121 ataccacacc ctgccaaagt tgggagatgc acttaaggtt gcacttgtgg tcgccaacta
      181 tgcctataca cgcggatttc ccgcgtttat aggccgcgac ggaagtctgg cttttaagcg
      241 aagacaataa ccgaaaagac cgcctcggcg gtctttcttg tgtacacagt ttttatattt
      301 gtcagcttga tgcattgaca actctaattc catattgcgg aatatattca tcgacagtca
      361 tcagttcaaa gccttccgct tgggtttgtg caatcaacat cctatcgaaa gggtctttgt
      421 gtatttccgg aaggcttcca gcctgttttg catgaaacag acctataggc agcatttcaa
      481 aatcctcttc ttgaagcaca tcaaaaaact cttccggtaa tttcaacaac cccttgttct
      541 gcttgatgga aatttcccaa atacttgctg cactgacaaa gatcgcattt ctcggatttt
      601 ctatcagttt gcgtgcagat gtccccagtt tcttgtcatc caacaaccac cacagcaaag
      661 catgggtatc aagcagaatc tttctcacag agccgactcc tcaaataaag ctgccgtttc
      721 attgtcatcc tcaagaatac gtgaaatatc cgtatttccc atatgactga attttttcaa
      781 ccttcctgca tttcgtgccg gtttttcaat accggttagt tggacgcaag gcttacctgc
      841 tttcgcaata ataacgattt cccctgcttc tgctctttga atcaattgac tcaaattggt
      901 ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct aacttaacta
      961 atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta tatccgccgt
     1021 tttattcaca ccttg
//
LOCUS       DAXHMX010000246         1007 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXHMX010000246 DAXHMX010000000
VERSION     DAXHMX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.246"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..448)
                     /gene="mafA"
                     /locus_tag="ACFM4S_002125"
     CDS             complement(<1..448)
                     /gene="mafA"
                     /locus_tag="ACFM4S_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6051722.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNG"
     gene            complement(571..960)
                     /locus_tag="ACFM4S_002126"
     CDS             complement(571..960)
                     /locus_tag="ACFM4S_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051723.1"
                     /translation="MWKITKEQGQDLSFATYCLLSAAINLQEFKLWLEKVVLDMPIDN
                     IHFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT          239 a          199 c          279 g          290 t
ORIGIN      
        1 tgccgttgac ggacagtccg gcggagcgtt cgcctttgcg tccgctgttt ctagtcaggg
       61 cggcggcggg ggcgtttaag agcgatgtgg aagtggttac gctggagagc gcgtcggatt
      121 tggtggtggc ggtagtgtcg taggcggggt agctgtattg ggtggcactt tcggggttgt
      181 tgtggtagcc accgcgtatc agtgcgtcga tagagtagcg tccgccgctt atgttgcccg
      241 aaccttggtc gcccataacg gagacgtaaa gggcggcttt gcgtcctttc agggcggaca
      301 aatccatttc tttgacggcg gcgcgggacg atgcggcgac gagttcctgt tcgacggcaa
      361 agcgtttgcc gccgccgtgg gcgggtatgc cggtcagtgt gccgcaggcg gttaggacga
      421 gggggatgag gaggagcagg gttttcatag cggggtttgt ttgatgttga acggattttg
      481 agtgtaaagg gattttaagg gtttgtaaac aaaaggggcg aaaatgccgt ctgaacggcg
      541 gaacggtctt tcagacggca ttttgactgt ttaaagcggg ggcagttcta caaacggaaa
      601 gaaatgctga aacctctgat aaatttcagg atgtttcttt aaggctttta atgctttttc
      661 ttttgaaacg ggagggtcat agacatctat accccttaag aaggcaatgc ctgttaatgc
      721 gtcatcttgg tctttagata agtctgaatt cggaacaaaa tccaaaatat tataaatatc
      781 tcctactcct tctttaagat caattaaatc aaaaatataa aaatgtatat tatcaatagg
      841 catatctaag acaaccttct ccaaccataa tttaaattct tgcaaattaa ttgctgccga
      901 taataagcaa tatgttgcaa aacttaaatc ttgaccttgc tctttcgtaa ttttccacat
      961 aattatcttc ctttgccgtc aaacgctctt ttggttacct gctttgt
//
LOCUS       DAXHMX010000247          988 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXHMX010000247 DAXHMX010000000
VERSION     DAXHMX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.247"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(17..955)
                     /locus_tag="ACFM4S_002127"
     CDS             complement(17..955)
                     /locus_tag="ACFM4S_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051724.1"
                     /translation="MDAYTEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT          182 a          280 c          315 g          211 t
ORIGIN      
        1 gtcgtctgaa aacgggctaa cggctgccca gcggcaatcc gatgtccgaa atcagcctgt
       61 cgggcaggac gtcgtcgcgg aagcggcaaa gctgcgcggg acggcctttg ctgcccgata
      121 cgccggtatc cgacggctcg ataaggtttt gctgctgaat ctggcggcgg aagttttgct
      181 tgtgcagcaa tcttccgctg atggcttcga cactgttttg cagttgcagc agcgtgaatt
      241 cgggcggcat cagttcaaaa atcacggggc ggtatttgat tttggcgcgc aggcgggaca
      301 gggcggtcgc cagtacgcga cggtgatcgt ggcgcatggg ctgccccgtg agcgcgaagt
      361 cgaagtttgc ctgcggctcg gcggcttcca ctatcagacc gctttcatac agcatttcat
      421 agcgttgtaa aacgtattct tccgaccagt tttccggttc gaccccccag cacaaatgaa
      481 tgcgcttgag ccgcatttga cgcacttcct ccgtgtccgc cgaatttgcc caaatgcgca
      541 ggcggctgac gacggcgtcg cgctgcccgc cgtcggtgcg caagtcttcc cacgggaaat
      601 agccgtagca gtcctgccat ttcgcatccg ggtgcaggat gctgtcggct gcctcgcgca
      661 ccagccccaa atagctgacg tacaagacgg gcatgccgtg ttcgttgcgg cggtgggtat
      721 cgacaaaggt gtaaagctgt tccacatagc ccataggctg cgaagtctgc ttggcaaccc
      781 acagcttcac gcccgcctgc agggaattgc gcagagggga gagcgggccg ttgggcagga
      841 gtgtgccttg ggcgacggtt aatacccgca ggccgccgtc ggtaacggca atcaatacgg
      901 gaaccagctc gacgatgctt tgcggcgggg cttcggcttc ggtgtaggca tccatgctcg
      961 gctttctgcg ggtgggaaag gctttatt
//
LOCUS       DAXHMX010000248          960 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXHMX010000248 DAXHMX010000000
VERSION     DAXHMX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.248"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            238..>960
                     /locus_tag="ACFM4S_002128"
     rRNA            238..>960
                     /locus_tag="ACFM4S_002128"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          265 a          189 c          297 g          209 t
ORIGIN      
        1 tgctgcagaa gcggcggacg aactgaaaag tatagcacag aatgttgggg atatcgagag
       61 atatcttgac aggcggaagg aatactttat aattcgcaac gctctttaac aaaacagatt
      121 accgataagt gtgagtgcct tgagcctcac actgtttgaa agacagacaa gataatgttt
      181 tgaacattgt cctgttggtt tctttgaagc agaccagaag ttaaaaagtt agagattgaa
      241 cataagagtt tgatcctggc tcagattgaa cgctggcggc atgctttaca catgcaagtc
      301 ggacggcagc acagagaagc ttgcttcttg ggtggcgagt ggcgaacggg tgagtaacat
      361 atcggaacgt accgagtagt gggggataac tgatcgaaag atcagctaat accgcatacg
      421 tcttgagaga gaaagcaggg gactttcggg ccttgcgcta ttcgagcggc cgatatctga
      481 ttagctggtt ggtggggtaa aggcctacca aggcgacgat cagtagcggg tctgagagga
      541 tgatccgcca cactgggact gagacacggc ccagactcct acgggaggca gcagtgggga
      601 attttggaca atgggcgcaa gcctgatcca gccatgccgc gtgtctgaag aaggccttcg
      661 ggttgtaaag gacttttgtc agggaagaaa aggctgttgc taatatcagc ggctgatgac
      721 ggtacctgaa gaataagcac cggctaacta cgtgccagca gccgcggtaa tacgtagggt
      781 gcgagcgtta atcggaatta ctgggcgtaa agcgggcgca gacggttact taagcaggat
      841 gtgaaatccc cgggctcaac ccgggaactg cgttctgaac tgggtgactc gagtgtgtca
      901 gagggaggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag gaataccgat
//
LOCUS       DAXHMX010000249          952 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXHMX010000249 DAXHMX010000000
VERSION     DAXHMX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.249"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            58..783
                     /locus_tag="ACFM4S_002129"
     CDS             58..783
                     /locus_tag="ACFM4S_002129"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6051725.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFDAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          251 a          328 c          212 g          161 t
ORIGIN      
        1 tgtccgtttc catcacgccg ctgcgacaga ttataaccgt ccgaaccgcc aaaaactatg
       61 ccccgattct acctgcccga aaacctttcc gtcggacaaa ccgtcgccct gcccgacaac
      121 atcgtccgcc acctcaacgt cctacgcgtc cgccccaacg aaaacatcac cctcttcgac
      181 ggcaaaggca aggcacacgc cgcacagctg accgttttgg aaaaacgccg cgccgaagcc
      241 gaaatcctgc acgaagacac aaccgacaac gagtccccgc tcaacatcac actgatacaa
      301 tccatctcct ccggcgatcg catggatttc accctgcaaa aaagcgtcga actcggcgta
      361 accgccatac agcccgtcat cagcgaacgc tgcatcgtcc gcctcgacgg agaacgcgcc
      421 gccaaacgcc tcgcacgctg gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg
      481 aacaccgttc cccccgtact gcccatcatc ggctaccgtg aagcactcga caaaatgccg
      541 tctgaaagca ccaagctgat tatgagcatc aaccgcgccc gcaaactcgg cgacatccgc
      601 caaccgtccg gcgcaatcgt ctttatggtc gggcctgaag gcggctggac agaacaggaa
      661 gaacaacagg catttgatgc cggctttcag gcggttacac tcggcaaacg gattttacgc
      721 acagaaaccg ccccactcgc cgccctcgcc gccatgcaga cgctttgggg cgatttcgca
      781 taaacagaaa tgccgtctga aacccgttca gacggcattt tgcagccgat taagatagta
      841 ggttcaaata agatttcccg tgtcgtcatt cccgcgaaag cgggaatcta gaaacgaaaa
      901 actacaggga tttatccgaa acaacaaccc tctccgccgt cattcccgcg ca
//
LOCUS       DAXHMX010000250          948 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXHMX010000250 DAXHMX010000000
VERSION     DAXHMX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.250"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..195
                     /locus_tag="ACFM4S_002130"
     CDS             <1..195
                     /locus_tag="ACFM4S_002130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051726.1"
                     /translation="IPAKAGIQPPLTQQEFIEKTETSKPSFPRKWESSNRKTTGIYRK
                     KPQPPKKPGGYRTILPNPDL"
     gene            278..448
                     /locus_tag="ACFM4S_002131"
     CDS             278..448
                     /locus_tag="ACFM4S_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC6051727.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT          286 a          250 c          208 g          204 t
ORIGIN      
        1 attcccgcaa aagcgggaat ccagcccccc ctaacgcaac aggaattcat cgaaaaaacc
       61 gaaacctcca aaccgtcatt cccacggaag tgggaatcca gtaatcgaaa aaccacagga
      121 atctatcgaa aaaaacccca acccccaaaa aaaccgggcg gataccgcac catcctccca
      181 aaccctgatt tatagtggat gaacaaaaat caggacaagg cgaggcaacg ccgtaccggt
      241 ttttgttcat ccgctataca aaaacaagga aacacaaatg gcgcaatata tgtgcggccc
      301 ctgcggctgg atttacgatg aagaacacgg cgaccccgaa cacggcatcc cccccgggac
      361 aaagtttgaa gacattcccg acgactggaa atgccccgaa tgcggcgtag gtaaagaaga
      421 tttctacctg ttggatttcg tgatatagcg gcagccgaac cccttcggaa aattcctcgg
      481 cgttccgcct gcggtagcag ggaacaccgt tttatagtgg attaacaaaa atcaggacaa
      541 ggcgacgaag ccgcagacag tacaaatagt acggaaccga ttcacttgtt aaagaatcgt
      601 tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaatccac tatatccgtt
      661 tgcgcccgcc aagccgtgtt gcacagatgc gacacggctt ttttctttct gttgtcagaa
      721 tgtaaatttt cgtcaaaaaa catcaaaaat cacccccccc ccgaatttgc gtaaaataaa
      781 gtgtaatccc tttgatcata agggaatgtt gacaattaga tcgattctcc taggctgact
      841 ttcgcaataa atggggttgt caataattgt gtatggtcgt ttgccgtccg gcggccgtca
      901 ttcccgcgaa cgcaggaatc cagcaacaca atgcagcagg actttatc
//
LOCUS       DAXHMX010000251          906 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXHMX010000251 DAXHMX010000000
VERSION     DAXHMX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.251"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(55..834)
                     /locus_tag="ACFM4S_002132"
     CDS             complement(55..834)
                     /locus_tag="ACFM4S_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6051728.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          222 a          291 c          179 g          214 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcaaccaaa acattcaatg ccgaccggac cgacgggcgc gaaacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg cagaaccctt ccgaaatata gtggattaac aaaaatcagg
      901 acaagg
//
LOCUS       DAXHMX010000252          905 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXHMX010000252 DAXHMX010000000
VERSION     DAXHMX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.252"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..215
                     /locus_tag="ACFM4S_002133"
     CDS             <1..215
                     /locus_tag="ACFM4S_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051729.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTRATLLALDWQGN
                     KPLGAAELADLKPLYKDLKNNIGNIV"
     gene            570..>905
                     /locus_tag="ACFM4S_002134"
     CDS             570..>905
                     /locus_tag="ACFM4S_002134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC6051730.1"
                     /translation="MYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDGYDVYRQLR
                     WQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELKAFPCLSSL
                     FLTKWHKNLY"
BASE COUNT          280 a          149 c          212 g          264 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacacg tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg cggaactggc ggatttgaaa ccgctttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgccgtc gtgccgacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atacccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcca aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaaatgg cataaaaatc
      901 tatat
//
LOCUS       DAXHMX010000253          896 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXHMX010000253 DAXHMX010000000
VERSION     DAXHMX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.253"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..>896)
                     /locus_tag="ACFM4S_002135"
     CDS             complement(<1..>896)
                     /locus_tag="ACFM4S_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6051731.1"
                     /translation="RAVPYVGTALLAHDVYETFKEDIQAQGYQYDPETDKFVKGYEYS
                     NCLWYEDKRRINRTYGCYGVDSSIMRLMSDDSRFPEVKELMESQMYRLARPFWNWHKE
                     ELNKLSSLDWNNFVLNRCTFNWNGGDCLVNKGDDFRNGADFSLIRNSKYKEEMDAKKL
                     EEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVVATFGRD
                     SQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTSPNPEPD
                     PDLNPDANPDTDGQPGTRPDSPA"
BASE COUNT          188 a          207 c          221 g          280 t
ORIGIN      
        1 gcgggggaat cgggtcttgt gccgggctgt ccgtccgtat cgggatttgc atcgggattc
       61 aaatcggggt cgggttcggg attggggctc gtgccggggt tctcattggg gttcgggttg
      121 tttgcggggt tttcggcggg cgatacttcg ggcagcggct gtgcgttcgg tgcttccgcg
      181 cttccggggg tcaagtcggg acgcgggatt acttgaacat ccaccgtggt gttgccttgc
      241 gaatccctgc cgaatgttgc gacaacctga acgggattcc cgttcctgtc cgtgacggga
      301 cccatattca cttttgttcc gggtgcgact tctacttttt cggaataacc gggataaccg
      361 gttgccttta tgtatttgtc gggattggca tcgactttca acgataaaat ctcttccagc
      421 tttttggcat ccatttcttc tttgtatttt gaattgcgaa taagggaaaa atcagcccca
      481 tttctgaaat catcaccttt attgaccaaa caatctccgc cattccaatt aaatgtgcaa
      541 cgatttaaaa caaaattatt ccaatccaaa gaacttaatt tattcagttc ttctttatgc
      601 caattccaaa acggacgtgc cagcctatac atttggcttt ccatcaattc tttgacttcg
      661 gggaatctgc tgtcatcgga cataaggcgc ataatcgaac tgtcaacgcc gtagcagcca
      721 taggttctat taatacgtct tttgtcttcg taccaaaggc aattactata ttcgtagcct
      781 tttacaaatt tgtcggtttc ggggtcgtat tggtagcctt gtgcctgtat gtcttctttg
      841 aaagtttcgt atacgtcatg ggctaaaagg gctgttccga cataaggaac tgccct
//
LOCUS       DAXHMX010000254          886 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXHMX010000254 DAXHMX010000000
VERSION     DAXHMX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.254"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(10..576)
                     /locus_tag="ACFM4S_002136"
     CDS             complement(10..576)
                     /locus_tag="ACFM4S_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6051732.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGS
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          236 a          282 c          188 g          180 t
ORIGIN      
        1 tttccgacat cattgaatca aacccaaatg cgacaagagc gtccatgtac cgatggcaat
       61 caacaccaaa cctccggcaa attccgcacg cctgccgaac aatacgccca aagcccttcc
      121 cgccgtcagc ccgaccgcca ccatcaccgt cgtcgccata ccgatgattg cggcggcaaa
      181 ggcgatgttt acctccataa acgccaagcc caccccgact atcatggaat caatactggt
      241 tccaaaagca gtcaaaaccg tcatccatag gctttcccgt ttgctttcgc gcacatcttc
      301 cgccttgccg gacagccctt cgcgcatcat tttcagaccc agcccgccca gcaggacgaa
      361 agccgcccaa tggtcccatt cgctgataaa cggtttggca taaaaaccgc ctacccagcc
      421 tgccagcggc gtgagtgctt caaccgagcc gaacaccaag gctgttgctg caattttgcg
      481 cggaggcatc ctgaccgccg caccctttgc caatgcgacg gcaaacgcat ccatcgacat
      541 ccccagcgca atcaagagca aagcgtaaag actcataccg cacccatcct caaaaagggc
      601 agattatagc aaaacagcaa agcgcgaaaa acgccgcacg aaaacccaca tcccgtcatt
      661 cccgcgcagg cgggaatcca gagttgtcgg tgcggaaact tatcgggaaa aacggtttct
      721 ttagatttta tagtggatta acaaaaacca gtacagcgtt gcctcgcctt ggctcaaaga
      781 gaacgattct ctaaggtgct gaagcaccga gtgaatcggt tccgtactat ctgtactgtc
      841 ttcggcttcg tcgccttgtc ctgatttttg ttaatccatt atatga
//
LOCUS       DAXHMX010000255          880 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXHMX010000255 DAXHMX010000000
VERSION     DAXHMX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.255"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..837
                     /locus_tag="ACFM4S_002137"
     CDS             <1..837
                     /locus_tag="ACFM4S_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6051733.1"
                     /translation="GCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQA
                     REDRNKGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQ
                     LGDKTSTWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFVQKATRIHAVKEDK
                     VISADRYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAF
                     LKAQAIHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPIS
                     WA"
BASE COUNT          293 a          206 c          191 g          190 t
ORIGIN      
        1 ggttgcggcg tcgcaaaaga aggctgggaa tcacgacttt tcgcattcct gactaatgca
       61 atccgcccaa aaatcacacg cgttgacatc gcaaaagact ttttcaacgg cgaatacagc
      121 ccgaaccaag cccgtgaaga ccgaaataaa ggtatgttta cctgtcatca cgtcaaacca
      181 aaaggcgaat gtttggggtc agattgggaa gaagacgatg aagccaaaat gaccaaaggc
      241 aagacctatg gtatcggctc ccgtgaatcg tccaaatatg tccgcgtcta tgaaaaaggc
      301 aagcagttgg gcgataaaac aagcacatgg acgcgatttg aaattgaatt caaagcaaaa
      361 gatatcgtta tccctttcga agttttgcag aatccgggcg aatatttcgg cggcgcatat
      421 ccgatttgcg aacgattcgt ccaaaaggca acgcgcatac acgcggttaa ggaagataag
      481 gtcatttcag ccgaccgcta ccttgaatgg gtaaaaaaac agttcggacg tgcggcaaac
      541 ggtctgaaat tcatttttcc cgaattggac aaagccaaac tgtttgaact gattgagccg
      601 agtcatcaca agctgcccaa gtctttggct cccgaagcct acgactgcgc ctttttgaaa
      661 gctcaagcca ttcatgaaca gcccgcattc aaaccgtaca aagaccctta ctatatgtac
      721 gaatattacg agaatcttga aaaacagctt gaacagcaaa aacacgtcaa caatgaagaa
      781 agctataaca acttcattta cgacaaattc gcaagactac cgatttcatg ggcttaaagt
      841 gtctgcccga aagacgttta atcacacaag gaaaccaaaa
//
LOCUS       DAXHMX010000256          841 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXHMX010000256 DAXHMX010000000
VERSION     DAXHMX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.256"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..472
                     /gene="mafA"
                     /locus_tag="ACFM4S_002138"
     CDS             <1..472
                     /gene="mafA"
                     /locus_tag="ACFM4S_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6051734.1"
                     /translation="LLANPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFG
                     TVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPY
                     SVGKTVKASDRLMVDFSDITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            476..>841
                     /gene="mafB"
                     /locus_tag="ACFM4S_002139"
     CDS             476..>841
                     /gene="mafB"
                     /locus_tag="ACFM4S_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6051735.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKG"
BASE COUNT          223 a          319 c          178 g          121 t
ORIGIN      
        1 cctgctcgcc aacccccgcg acgtttcctt cctgaccaac ctcatccaaa ccgtcttcta
       61 cctgcgcggc atcgaagtcg taccgcccga atacgccgac accgacgtat tcgtaaccgt
      121 cgacgtattc ggcaccgtcc gcagccgcac cgaactgcac ctctacaacg ccgaaaccct
      181 taaagcccaa accaagctcg aatatttcgc cgttgaccgc gacagccgga aactgctgat
      241 tgcccctaaa accgccgcct acgaatccca ataccaagaa caatacgccc tctggatggg
      301 accttacagc gtcggcaaaa ccgtcaaagc ctcagaccgc ctgatggtcg atttctccga
      361 catcaccccc tacggtgaca caaccgccca aaaccgtccc gacttcaaac aaaacaacgg
      421 taaaaacccc gatgtcggca acgaagtcat ccgccgccgc aaaggaggat aaaccgtgaa
      481 accgctgcgc agactgacaa acctccttgc cgcctgcgcc gtagcggccg ccgccttcgg
      541 acagcccgcc ctcgcggcgg acttggcgca agacccgttc attaccgata acgcccaacg
      601 gcagcactac gaacccggag gcaaatacca cctcttcggc gacccgcgcg gcagcgtttc
      661 cgaccgcacc ggtcaaatca acgtcatcca agactatacc caccagatgg gcaacctgct
      721 catccaacag gcaaacatca acggcacaat cggctaccac acccgctttt ccggacacgg
      781 acacgaagaa cacgccccct tcgacaacca cgccgccgac agcgcaagcg aagaaaaagg
      841 c
//
LOCUS       DAXHMX010000257          839 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXHMX010000257 DAXHMX010000000
VERSION     DAXHMX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.257"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..>839)
                     /gene="mafB"
                     /locus_tag="ACFM4S_002140"
     CDS             complement(<1..>839)
                     /gene="mafB"
                     /locus_tag="ACFM4S_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6051736.1"
                     /translation="VYAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHEVHS
                     PFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARDIYS
                     YYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNWWAN
                     RMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLSPEA
                     QLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAATTVWGGKKVELN
                     PTK"
BASE COUNT          196 a          209 c          193 g          241 t
ORIGIN      
        1 atttggtcgg gttaagttct acttttttac cgccccaaac cgtagttgcg gcctctgcta
       61 cggaaagggc agtttgggca gtagctgtaa tgttcggatg ggcatcagcc cattgtctgg
      121 cagagttgat actgtctttt accgcaaaag cactgtcttg taatgcggtt gcggccgcaa
      181 gttgtgcttc cggacttaaa tttcctaaat tgtgaatacc ctgtaaagtt ttccgtgctg
      241 ccgcataggt taccgggttt accgcactgt ctgtaattgc cccaactccc aatccctgaa
      301 aacccattaa aaaaggatta accgcacctt ggacgatgcc gcgaatatca tccatacggt
      361 tagcccacca atttttattg gggtcgcttt cccatatcag ttttcctgct tcatccgcac
      421 ggctgaaaaa accagaggcg gcaccggcat tttcttttag ccaacggtct gaaaatgggg
      481 cttgagggac aatattagtc tttgtttttg ttgaagttcc tttgacataa tagctgtata
      541 tatcccttgc tcctccgggg ggcggataat cgctgccttg cggcccgtca tatccatcct
      601 ccggatgaat ttccgaccct gtccgatgaa gttggtaaac agtaaaacca ccgtctacac
      661 cgccgctgaa atcagaagtg ctttttgaat catgattatc gaacggactg tgtacttcat
      721 gtccatgtcc tgaaaaatgg gtttcataac cgataatgcc ttcaaaccct gtccgttcgt
      781 gtgtaatagg cagtatgggg ccgaccgcag ttgcatcaaa tgtctggacg gcgtaaacc
//
LOCUS       DAXHMX010000258          824 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXHMX010000258 DAXHMX010000000
VERSION     DAXHMX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.258"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            370..615
                     /locus_tag="ACFM4S_002141"
     CDS             370..615
                     /locus_tag="ACFM4S_002141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051737.1"
                     /translation="MVLELSTYCVSPSAGSVVDFDLKKRGGVGQDVFRGTPDCGGVCL
                     YFAEGFKNRVVGYIGCTVLVCARSGQGAMGKFARDAV"
     gene            complement(635..>824)
                     /locus_tag="ACFM4S_002142"
     CDS             complement(635..>824)
                     /locus_tag="ACFM4S_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051738.1"
                     /translation="TIMRPFDGVKYKDTLTAGFSSPHHHLTADSYLQKRREPDRWYSR
                     IRRAVREAATSRAPSHDE"
BASE COUNT          148 a          159 c          294 g          223 t
ORIGIN      
        1 gcggccggtg cttttttgtt tggaagggaa acggcaggaa aaagacccgc gccatatcgg
       61 ggaatgaacc gacgcaaagg aaatgcggtt tgaatatgcg tccgggcggt gttttactgt
      121 gtgtgccgta tggcggggca gggtgtctga tttcgtgatg accgtgttgg tggaatcgaa
      181 gcctgccgtt ccggtaaggg ttgcccggga gggcaaggcc ggctttgtat gcccggttaa
      241 gagggaagga tgagactgtg catttttgat tttaattggc tatatgccgt ctgaaaatcg
      301 gtttgaaggc ggtactttta tgctgccttt catacagggg aatgggaacg ttgcggctgc
      361 gcggaaaccg tggtgcttga actttctact tactgcgttt cgccgtcggc gggttcggtg
      421 gtggattttg acctgaaaaa aagggggggt gttgggcaag atgtttttag gggaacgccg
      481 gattgcggtg gggtttgcct ttattttgca gaaggcttta aaaaccgtgt ggtcgggtac
      541 atcggctgta ccgtgcttgt ttgtgcaaga tcggggcagg gtgcgatggg caagtttgcc
      601 cgggatgccg tctgaagcgt tcagacggca ttttttattc gtcgtggctt ggtgcgcggg
      661 atgtggcggc ttcccggacg gcacggcgga tgcggctgta ccatcggtcg ggttctcgcc
      721 gcttttggag gtaactgtcg gcggtcaggt ggtggtgcgg gcttgaaaaa ccggcggtta
      781 gggtgtcttt atatttcacc ccgtcaaaag ggcgcattat agtg
//
LOCUS       DAXHMX010000259          805 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXHMX010000259 DAXHMX010000000
VERSION     DAXHMX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.259"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..128)
                     /locus_tag="ACFM4S_002143"
     CDS             complement(<1..128)
                     /locus_tag="ACFM4S_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6051739.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQKT"
     gene            complement(249..560)
                     /gene="rpsJ"
                     /locus_tag="ACFM4S_002144"
     CDS             complement(249..560)
                     /gene="rpsJ"
                     /locus_tag="ACFM4S_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6051740.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(578..>805)
                     /locus_tag="ACFM4S_002145"
     CDS             complement(578..>805)
                     /locus_tag="ACFM4S_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6051741.1"
                     /translation="HTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVEL
                     IAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT          202 a          182 c          203 g          218 t
ORIGIN      
        1 gttttttgac ggttcaggta ctgctcgatc ggctgccaat caatcacctg gtccaacttc
       61 aatagcggga aacggtcgat gtgtctggca atcatggctt gggcggtttg ctggaagaag
      121 gtgctcatga gaaatcccct aaatgtcttg gtgggaattt agaggatttt ggggaatttt
      181 gcaaaggtct cggcataaca taaaaaatcc ccaagcattg attgcttggg gattttcaat
      241 agtccgaatt attggacttt gatttctaca tcaacaccgg ccggcaaatc cagcttcatc
      301 agcgcatcgg tagttttatc ggtccaatcc acgatgtcca tcaggcgcaa gtgggtgcgg
      361 atttccaatt gctcacggga agttttgttc acgtgcggag aacgcaaaat gttgaaacgc
      421 tcgattttgg tcggcaaagg aatcgggcct tttacaactg caccggtacg ttttgcagtt
      481 tcaacgattt cttgtgcaga acggtcaatc agggcgtaat cataagcttt caggcggata
      541 cggatttttt ggtttgccat ttatcaatat ccttcaatta agcgataaca gaagaaacca
      601 cgccggcacc cacggtacgg ccgccttcgc gaatcgcaaa gcgcaaacct tcttccatag
      661 cgataggcgc aatcagttct acggtgatgg ttacgttttc acccggcatt accatttcta
      721 caccttcttc caaagtaacc gcgccagtta cgtcggtggt acggaagtag aattgcggac
      781 ggtagttagc gaagaacggg gtatg
//
LOCUS       DAXHMX010000260          802 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXHMX010000260 DAXHMX010000000
VERSION     DAXHMX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.260"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>802
                     /locus_tag="ACFM4S_002146"
     CDS             <1..>802
                     /locus_tag="ACFM4S_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6051742.1"
                     /translation="LAENQSRYDTWKEGGIGRSILHGAAGGLTTGSLGGILAGSGTSL
                     AAPYLDKAAENLGPAGKAAVNALGGAAIGYATGGSGGAVVGANVDWNNRQLHPSEYKL
                     AEQYADIVASRLNISKEEALGRIIRHMQRTVDYATAKADNFRTDDAIISILGANNLPK
                     QDQHYTNPNYNKQYIQNYVRDFNLANKYNYFGKTPTQSRDYKVGVVTDIGKAALNSGI
                     GIFESAANLATGEIDPNSWAYVHMGRIGYTHPEYGNAWIRISSATLVY"
BASE COUNT          242 a          164 c          207 g          189 t
ORIGIN      
        1 accttgccga aaaccaaagc cgctacgaca cctggaaaga aggcggcata ggcaggagca
       61 tactgcacgg ggcggcaggc ggactgacga ccggcagtct cggcggcata ctggccggca
      121 gcggcacttc ccttgctgca ccgtatttgg acaaagcggc ggaaaacctc ggtccggcgg
      181 gcaaagcggc ggtcaacgca ctgggcggtg cggccatcgg ctatgcaact ggtggtagtg
      241 gtggtgctgt ggtgggtgcg aatgtagatt ggaacaatag gcagctgcat ccgagtgaat
      301 ataaattagc agaacaatat gcagatatag tggcttctcg tctaaacatt tcaaaagaag
      361 aagcacttgg gcgaattatt cggcatatgc aacgaaccgt agattacgca acggctaaag
      421 cggataattt ccgtactgat gacgccatta tttcaatttt aggagcaaac aatttaccca
      481 agcaagatca acattacact aatccgaact acaacaaaca atacattcaa aattatgttc
      541 gtgatttcaa tttagccaat aaatacaatt attttgggaa aactcctact caatcaaggg
      601 attataaagt tggagttgtt acagatattg gtaaggctgc attaaatagc ggtataggta
      661 tatttgaatc ggctgcgaat ttagcgacag gggagataga tccgaactca tgggcttatg
      721 tacacatggg gcgtataggt tatacacatc ctgaatatgg aaatgcttgg attcggattt
      781 caagtgcaac actagtgtat ta
//
LOCUS       DAXHMX010000261          798 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXHMX010000261 DAXHMX010000000
VERSION     DAXHMX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.261"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            409..615
                     /locus_tag="ACFM4S_002147"
                     /pseudo
     CDS             409..615
                     /locus_tag="ACFM4S_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            630..798
                     /locus_tag="ACFM4S_002148"
                     /pseudo
     CDS             630..798
                     /locus_tag="ACFM4S_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
BASE COUNT          216 a          219 c          172 g          191 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacaaga aaaaactaca ccaaactaca
      121 atttttgttt atacaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 attctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttctttgc cgcgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgtattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cactgaccca acgcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggcaaat ccacccgcat tggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc cctatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaac
//
LOCUS       DAXHMX010000262          712 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXHMX010000262 DAXHMX010000000
VERSION     DAXHMX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.262"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..116)
                     /locus_tag="ACFM4S_002149"
     CDS             complement(<1..116)
                     /locus_tag="ACFM4S_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6051743.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNS"
     gene            complement(236..694)
                     /locus_tag="ACFM4S_002150"
     CDS             complement(236..694)
                     /locus_tag="ACFM4S_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051744.1"
                     /translation="MPSEHAFGRRFVGGYRSGTSEKGFQAVFGRVVTVENVIRLPEKF
                     GRFSGGLWYWAQQTQVQIRRCAVIVRMPIQPKQTSVFRKWMFDGAKVVRRMEGNLWVF
                     GMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          189 a          184 c          127 g          212 t
ORIGIN      
        1 gaattgggat ggatacccaa aatatcggcg gcagaacggg cggtaacttc cagtacaaaa
       61 aaacggagca gttctttctg tacttttttc tttaatttgc agtgcgttat cttcatattt
      121 cgagggtaac atatctgcta atctagtaca gcccctagtt taaagctttg tttcttaagt
      181 ccgcagagta tgccatggtt agaccttcaa agttgagtat tgtactattt tgtttttaat
      241 taactataaa acgcttgtgt gaataggaag actgcagaat attgctgcct tttcctgttt
      301 gctttaaata aacagaaaat gccgtctgaa accgattttc agacggcatt tgtcccttaa
      361 aacacgtttt ttcaagcgcg accataccaa acacccacag gttgccttcc atccttcgta
      421 caacctttgc tccatcaaac atccacttcc taaatactga tgtctgctta ggttgaatcg
      481 gcattcgtac gattacggca caccgcctaa tctgtacctg cgtctgttgc gcccaatacc
      541 aaaggccacc tgaaaatctc ccaaactttt caggtagcct tatcacgttt tcgactgtta
      601 ccacacgccc aaagaccgcc tgaaaccctt tttcagacgt tccgctccga taccccccaa
      661 caaaacgccg cccgaacgca tgttcagacg gcattttctt tgcgtcgatt ca
//
LOCUS       DAXHMX010000263          708 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXHMX010000263 DAXHMX010000000
VERSION     DAXHMX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.263"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT          183 a          165 c          199 g          161 t
ORIGIN      
        1 cttagaataa cagcaatatt caaagattat ctgaaagtcc gggattctag attcccactt
       61 tcgtgggaat gacggaatgt aggttcgcgg gaatgacgcg gtgcaggttt ccgtatggat
      121 gggttcgtca ttcccgcgca ggcgggaatc tagaccttag aataacagca atattcaaag
      181 attataaaag acccgtcatt cctgcgcagg cgggaatcca gaacgtaaaa tctaaagaaa
      241 ccgttttacc cgataagttt ctgtgccgac agacctagat tcccactttc gtgggaatga
      301 cgggatgtag gttcgtggga atgacgcggt gcaggtttcc gtgcggatgg attcgtcatt
      361 cccgcgcagg cgggaatcca gaccttggga taacagcaat attcaaaggt tataaaagac
      421 ccgtcattcc cgcgcaggcg ggaatccaga ctgtcgggca tctgcagcgg tttgctaaaa
      481 aacgctttac cgtgatcagt gtgcaaagtt aaaatgggga ggtaagcttt tcaatcagca
      541 attcggcggg cgcggaatcg ggcggtttgt ccaatcccgc cgtttgcggc gtgcctgccg
      601 ccgccggtat cccgcgaagg aagattgaag gaataaaata tgtttcaaca cgcagggcgg
      661 cacatcaagc accgccctat gtgccgccct gattcggaag gggctgca
//
LOCUS       DAXHMX010000264          685 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXHMX010000264 DAXHMX010000000
VERSION     DAXHMX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.264"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..264)
                     /locus_tag="ACFM4S_002151"
     CDS             complement(<1..264)
                     /locus_tag="ACFM4S_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFC6051745.1"
                     /translation="MFRILIISFLFLLSAKSFSADLEIKNGKLMYALSEKYNDNGFKA
                     YKVLGEGGGIHTEYNYKFDKSLNLNVLESSTGARSLEKVPVKVT"
     gene            complement(218..508)
                     /locus_tag="ACFM4S_002152"
     CDS             complement(218..508)
                     /locus_tag="ACFM4S_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051746.1"
                     /translation="MNKNERDFFYISNSDLDKLSESYPDRPLSYVFYCYLKETGLLKN
                     FSMDKCHNFFNRINFNESCFEIKFKDDSFFIIGNGKIDVSDSNNFFSVSFEC"
BASE COUNT          258 a          126 c           82 g          219 t
ORIGIN      
        1 agttacttta acggggactt tttcaagaga gcgtgcgcct gttgaacttt ctaatacatt
       61 caaattcaaa cttttatcaa atttgtaatt atattctgta tgaattcctc ctccctcacc
      121 taaaactttg tatgccttaa atccattatc gttatatttt tccgaaagtg catacatcaa
      181 tttcccattt ttaatttcta aatctgctga aaaagattta gcactcaaaa gaaacagaaa
      241 agaaattatt agaatccgaa acatcaattt ttccattgcc aataatgaaa aatgaatcat
      301 ccttgaattt aatttcaaag caagattcat taaaattaat tctattaaaa aaattatgac
      361 atttatccat tgagaaattt ttcaatagac cagtttcttt caaataacaa taaaacacat
      421 aagaaagagg cctatcagga taagattctg acaatttatc taaatcagaa tttgatatat
      481 agaaaaagtc acgttcattt ttattcatgg tttcaagcct caaatgtcta attaaaagcc
      541 tgattttgac accataactt catgcgctca attcttaaac agaaccgccc cgattaatac
      601 gggtacggaa acgccgagat aaaaataaaa atccatcatt tcaaaacctt tttcagcagg
      661 gaaacaaagt aaacggactc catca
//
LOCUS       DAXHMX010000265          679 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXHMX010000265 DAXHMX010000000
VERSION     DAXHMX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.265"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(80..565)
                     /locus_tag="ACFM4S_002153"
     CDS             complement(80..565)
                     /locus_tag="ACFM4S_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6051747.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMDQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          200 a          218 c          120 g          141 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatttt caaaccggaa aaatgccgtc tgaaccttca
       61 gacggcattc cattcacatt tattcgtctt ttttgtgttc gatggcgttt cgggaacgat
      121 tgccgccgta aggctcttca accgtatatt ccccctcgat aatatcgtca tcgcgggaaa
      181 agccctcttt tctgcccgat tggtccatgt tgaaaaaatt ttccgcacct cctgcctgca
      241 acactgcacc tcccttaaac ggcagcagca gcaacaccgc caacaccgag gatacgaatc
      301 ccggactcat cagacacacc gccgccaccg tataacggat aggccacaac atctgataaa
      361 cggataccct cccgccgctt ctcattgccg cgcccgccaa taaaagaccg gacagccccg
      421 tatgcctgag catcagcaca ccggcggcaa aacctgccgc catcaaaaac aacgtccagc
      481 cgccgcccaa ccaatcggca acccacacaa tcgacataat ctccaaaaac agcagcacca
      541 aaaaaccgat accgaaaaat ctcattgacc gtcatcctta tatttaagta aacagcaaac
      601 cgcccgaaca ggactccaag cgacctgcct gtaaatgatt acaaaaccat gtgcttcaag
      661 ccgaaacaat gtgaaatca
//
LOCUS       DAXHMX010000266          675 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXHMX010000266 DAXHMX010000000
VERSION     DAXHMX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.266"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(89..640)
                     /locus_tag="ACFM4S_002154"
     CDS             complement(89..640)
                     /locus_tag="ACFM4S_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051748.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNILQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAVKAGG"
BASE COUNT          187 a          271 c          127 g           90 t
ORIGIN      
        1 aagaaaccgc cattagcact acaccccccc aagctgcggc tgccaaacac atcggctgct
       61 tctcgacgca cccgctgaaa aatgcgccct atccgcccgc cttgaccgca ccccctgcct
      121 ttgccgccct gcccgatgcc gtctgaagcc tgaaccgccc caccagcccg atgacgcacg
      181 acaccgccgg aaagggcata cacaaagccg tcagcacata acgcgcattc cccgtattga
      241 ccacgtccgc gcccttcagc agcagcggca gcgcgttcgc ccactcccac accgacggcg
      301 cactgaacgc caacaccgcc accagcccca acatccggaa caccccgatt tccaacacct
      361 gccgccgccg caccgaacgg ttgagcagaa acacaaccgc caacccgaaa cagaccaccg
      421 ccgccatacc gccgttggcg acaatctgca aactgttgaa tgcaatatcg ggcgtaaccg
      481 ccaacacgcc ttccgaatcc ggcacggcac gctgcaaatc caaaccctgc aagatattca
      541 acacaaaacc gtagatcaaa tcaaaaaaca ccacgcctat cggcaaagca ctcaaaaccc
      601 gccacatctt cctgtccgac caaccgttca aaactttcat tttacccgac cacgcaagcc
      661 gccgaacaaa aaata
//
LOCUS       DAXHMX010000267          637 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXHMX010000267 DAXHMX010000000
VERSION     DAXHMX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.267"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            153..389
                     /locus_tag="ACFM4S_002155"
     CDS             153..389
                     /locus_tag="ACFM4S_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051749.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            361..>637
                     /locus_tag="ACFM4S_002156"
     CDS             361..>637
                     /locus_tag="ACFM4S_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051750.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKAAFAKQQAVID
                     KMEHDKAQALLLSAQNYARELELARAEAKKYEVKAHAVGMALAKKQAE"
BASE COUNT          157 a          142 c          206 g          132 t
ORIGIN      
        1 ttttacgaaa agatgattga gctgtggtcg agcaaaagcc gtgaggcaag cgaacgggct
       61 gatttggcgg cgtttgaatt tgcggagggc gaactggcca attatcggga aatgctgaaa
      121 cggcacctgc aaaccaaaag tgtggaatag caatgcgtat tttggatatt tttaaaaacc
      181 cggcgacagg caatgtgtcg cactcgaagc tgtgggcaaa cgtcgcctgc gcggcgggga
      241 cgtttaaatt tgtgatgttg cccgacccgt cggcggagat ttgggcggtg tatttgggca
      301 ttgtcggcgg ctatgccgtg gcgcgctcgt ttgtcagcgt caaacgtcag gaggtcgaga
      361 atgaatctcg tgaaactgct ggcgaataac tggcagccga ttgccatcat cgcgcttgtc
      421 ggcacgggtt tggcggtgtc acaccatcaa ggctacaagg cagcctttgc gaagcagcag
      481 gcggtcatcg acaagatgga gcacgataag gcgcaagccc tgctgttgtc ggctcaaaac
      541 tatgcgcgcg aactggaact ggcacgcgcg gaagcgaaaa aatatgaagt caaggcgcac
      601 gctgtcggca tggctttggc gaaaaaacag gcggaag
//
LOCUS       DAXHMX010000268          634 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXHMX010000268 DAXHMX010000000
VERSION     DAXHMX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.268"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT          155 a          147 c          181 g          151 t
ORIGIN      
        1 ccgtcattcc cgcgcaggcg ggaatctaga cattcaatgc taaggcaatt tatcggaaat
       61 gactgaaact caagaaactg gattcccact ttcgtgggaa tgacgggatg caggttcgtg
      121 ggaatgacgt ggtgcaggtt cgtaggaatg acgtggtgca ggtttccgtg cggatggatt
      181 cgtcattccc gcgcaggcgg gaatccagac attcaatgct aaggcaattt atcgggaatg
      241 actgaaactc aaaaaactgg attcccactt tcgtgggaat gacgcggtgc aggtttccgt
      301 acggatggat tcgtcattcc cgcgcaggcg ggaatctagg atgcggaatc tcaagaaacc
      361 gttttgcccg ataagtttct gcaccgacag gtctggattc ccgcctgcgc gggaatgacg
      421 attcgggtat ttctgacggt tcgggcattc ccgacaaggt ggattttcaa ggtgttgtat
      481 agggtgtagg aggattcgta aaaggtgagt tatagggtgg gcttcagccc accgattcca
      541 acgattccac caatcctaca ccgttcccat agactcaaat caacacagaa acttatgcgc
      601 cgtcattccc gcgcaggcgg gaatctagga tgcg
//
LOCUS       DAXHMX010000269          609 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXHMX010000269 DAXHMX010000000
VERSION     DAXHMX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.269"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(152..>609)
                     /locus_tag="ACFM4S_002157"
     CDS             complement(152..>609)
                     /locus_tag="ACFM4S_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6051751.1"
                     /translation="RHSNGNWKELKTENQGNATFHAASSLGLSAIYDFKLNDKFDKFK
                     PYIGARVAYGHVKHQVHSVETTTETIFTTPTKGAKVGGKIPNTPSSKPPYHQSHSISS
                     LGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
BASE COUNT          124 a          126 c          182 g          177 t
ORIGIN      
        1 agattgcggt attgtcggga atggcgggtt tcaagattac ggtgttgtcg ggaatgacgg
       61 tttgggtatt tccacgcccg ccccgcgttt gtcaacggca ggtgcatcaa aaatgccgtc
      121 tgaaggttca gacggcatcg gtatcgggga atcagaagtg gtagcgcatg cccaatgaga
      181 cttcgtgggt tttgaagcgg gtgttttcca agcgtcccca gttgtggtag cggtatccgg
      241 tgtctaaagt cagcttgggc gtgatgtcga aaccgacacc ggcgatgaca ccaagaccca
      301 agctgctgat actgtggctt tggtgatagg gaggtttgct actcggtgtg tttgggatct
      361 tgcctcccac tttagcgcct ttcgttggtg tagtgaaaat agtctcggtt gttgtttcca
      421 ccgaatgaac ctgatgttta acgtgtccgt aggcgacgcg cgcaccgata tagggtttga
      481 atttatcgaa tttatcgttg agtttgaaat cgtaaatggc ggataagccg agagaagaag
      541 cggcgtggaa tgtagcgttt ccctgatttt ccgtcttcag ttctttccag ttgccattgc
      601 tatgtcttt
//
LOCUS       DAXHMX010000270          582 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXHMX010000270 DAXHMX010000000
VERSION     DAXHMX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.270"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT          187 a          124 c          137 g          134 t
ORIGIN      
        1 ggtctgctca aagtgagtgc gcaaagccat ctgaaggcga tgtgtttgaa cctgttgaaa
       61 gccgccaaca agctaagtgc gcccgctgcc gcctaaaagg agaccggatg cctgattatc
      121 gggtatccgg ggagggttaa gggggtattt gggtaaaatt aggaggtatt tggggcgaaa
      181 atagacgaaa acctgtgttt gggtttcggc tgtcgggagg gaaaggaatt ttgcaaagat
      241 ctcatcctgt tattttcaca aaaacagaaa accaaaaaca gcaacctgaa attcgtcatt
      301 cccgcgcagg cgggaatcca gacccccaac gcggcaggaa tctatcggaa ataaccgaaa
      361 ccggacgaac ctagattccc gctttcgcgg gaatgacggc agagtggttt cagttgctcc
      421 cgataaatgc cgccatctca agtctcgtca ttcccttaaa acagaaaacc gaaatcagaa
      481 acctaaaatt tcgtcattcc cataaaaaac agaaaaccaa gtgagaataa caattcgttg
      541 taaacaaata actatttgtt aatttttatt aatatatgta aa
//
LOCUS       DAXHMX010000271          579 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXHMX010000271 DAXHMX010000000
VERSION     DAXHMX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.271"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            170..>579
                     /locus_tag="ACFM4S_002158"
     CDS             170..>579
                     /locus_tag="ACFM4S_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6051752.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRS"
BASE COUNT          195 a          115 c          116 g          153 t
ORIGIN      
        1 tccgcaaaat cacgacacac tcggacaccc gccccgctta tcgcaacttt gcgaacgccc
       61 cggaaacagc aaatacagca aataattgat tttattagag tttatttgca aaaccatttg
      121 ccgttacaca agaatggcac attaaaatga ctgatgagga tttataacga tgaagacaga
      181 cattcaaacc gaattaaccc aagccctact accacacgaa aaagtatggg cgaacgaaga
      241 aaaaaccatt ttagccaaaa acattctgtt ggatttggtg gaaaaaaccg acccgaccat
      301 tatcggtttg ttattgaata atgatgagtt aaaacgccat ttctttgtgg aagtgaatgg
      361 tgttttggtg tttaaattgc aggatttacg ttttttcttg gacaaacaca gcgtcaataa
      421 ttcctacaca aaatacgcca accgcattgg tttgacggac ggcaaccgct ttttgaaaga
      481 caattcggat attgtgttgg attttccgtt taaagattgc gtattgaatg gcggacaaag
      541 caccgaagaa ggcgaagaga tttattttaa acgcagtaa
//
LOCUS       DAXHMX010000272          578 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXHMX010000272 DAXHMX010000000
VERSION     DAXHMX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.272"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(131..424)
                     /locus_tag="ACFM4S_002159"
     CDS             complement(131..424)
                     /locus_tag="ACFM4S_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC6051753.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
BASE COUNT          165 a          127 c          100 g          186 t
ORIGIN      
        1 ccttggctgc ggtggtcgaa caatgcacct gcaaagcctt tggcggcgac agtttctatg
       61 ccgtcaccgt ccaaatctag ggcaaggggg tcgtaaacgt tatatttgcc tgaacgtttg
      121 agagctaatt tcaaccactc tcttgcccat tcagggaatt caggcagcca atcttcaaat
      181 ttcggaaatt ggtcccattc ttttcaagat aagggagaaa atcttgttta taccattttt
      241 ccagtaactc tccccattca cttatccctc gtccaatata atcttcgccc cctttccaag
      301 ggtttgcatc atcaggaact aaggaatata taaacctaga tacttcttct aatacattgc
      361 tgtatgtatc aggatccgca aaaaagtttg ctatcttact tggtgtaaaa tcatctatat
      421 tcattataga ttcgccaagc ctctcgtttc cataatcctt atttaattca attcatgctg
      481 taaaacagta gaggatttta gcatttttct cgtatatctc gagatacatt tcttaaattt
      541 aaaaaaatgt tatcgaggga tgcaagtaaa gaagcagt
//
LOCUS       DAXHMX010000273          567 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXHMX010000273 DAXHMX010000000
VERSION     DAXHMX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.273"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..420
                     /locus_tag="ACFM4S_002160"
     CDS             <1..420
                     /locus_tag="ACFM4S_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISNme1 family transposase"
                     /protein_id="HFC6051754.1"
                     /translation="RTDAEGYIEKLHITPANAHECKHLSPLLEGLPEGTTVYADKGYD
                     SAENRQHLEEHQLLDGIMRKACRNRPLSEVQTKRNRYLSKTRYVVEQSFGTLHRKFRY
                     ARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          147 a          140 c          162 g          118 t
ORIGIN      
        1 cgtaccgatg cggaaggcta tatcgagaaa ctgcacatca cccccgccaa tgcccatgag
       61 tgcaaacacc tgtcgccgtt gttggaaggg ttacccgaag gtacgaccgt ctatgccgac
      121 aaaggctacg acagtgcgga aaaccggcaa catctggaag aacatcagtt gttggacggc
      181 attatgcgca aagcctgccg caaccgcccg ctgtcggaag tgcaaaccaa acgcaaccga
      241 tatttgtcga agacccgtta tgtggttgaa cagagcttcg gtacgctgca ccgtaaattc
      301 cgctacgctc gggcagccta tttcggactg attaaagtga gtgcgcaaag ccacctgaag
      361 gcgatgtgtt tgaacctgtt gaaagccgcc aacaggctaa gtgcgcccgc tgccgcctaa
      421 aaggcagccc ggatgcctga ttatcgggta tccgggcagg attaagggga tatttgggta
      481 aaattaggag gtatttggta cgaaaacagc cgaaatcctg tgtttgggtt tcggctgtcg
      541 ggagggacag gaattttgca aaggtct
//
LOCUS       DAXHMX010000274          550 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXHMX010000274 DAXHMX010000000
VERSION     DAXHMX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.274"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>550
                     /locus_tag="ACFM4S_002161"
     CDS             <1..>550
                     /locus_tag="ACFM4S_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6051755.1"
                     /translation="NLFCRFDELSIPDYSTLCRYRNWLAQDDTLSELLKLINRQLTEK
                     GLKIEKASAAVIDATIIQTAGSKQRQAIEVDEEGQVSGQTTPSKDKDARWTKKNGLYR
                     LGYKQHTRTDEEGYIKKLHITPANAHECKHLSPLLEGLPKGTTVYADKGYDSAENRQH
                     LEEHQLLDGIMRKACRNRPLSET"
BASE COUNT          172 a          160 c          128 g           90 t
ORIGIN      
        1 aacctgtttt gccgtttcga cgaactgagc atccccgatt acagcacctt atgccgctac
       61 cgcaactggc tggcgcaaga cgacaccctg tccgaattgc tcaaactgat taaccgccaa
      121 ctgaccgaaa aaggtctaaa aatagagaaa gcatccgccg ccgtcattga cgccaccatt
      181 attcagaccg ccggcagcaa acagcgccag gccatagaag tcgacgagga aggacaagtc
      241 agcggccaaa ccacaccgag taaagataaa gatgcccgtt ggacaaagaa aaacggttta
      301 tacagactcg gttacaaaca acatacccgt accgatgagg aaggctatat caagaaactg
      361 cacatcaccc cagccaatgc ccatgagtgc aaacacctgt cgcctttgct ggaaggactg
      421 cccaaaggta cgaccgtcta tgccgacaaa ggctacgaca gtgcggaaaa ccggcaacat
      481 ctggaagaac atcagttgtt agacggcatt atgcgcaaag cctgccgcaa ccggccgctg
      541 tcggaaacgc
//
LOCUS       DAXHMX010000275          547 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXHMX010000275 DAXHMX010000000
VERSION     DAXHMX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.275"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..537
                     /locus_tag="ACFM4S_002162"
     CDS             <1..537
                     /locus_tag="ACFM4S_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6051756.1"
                     /translation="FGGWRIAADYARYRKWNNNKYSVNTKNVQKNDNGNRQDLKTENQ
                     ENGTFHAVSSLGLSAIYDFKLNDKFDKFKPYIGVRVAYGHVRHSIDSTKKTTNVVTVA
                     GAANTAPTIYYAPETQNAYHESHSIRRLGLGVIAGVGFDITPKLTLDTGYRYHNWGRL
                     ENTRFKTHEVSLGMRYRF"
BASE COUNT          167 a          153 c          114 g          113 t
ORIGIN      
        1 tttggcggct ggagaatagc ggcagattat gcccgttaca gaaaatggaa caacaataaa
       61 tattccgtca acacaaaaaa tgtgcaaaaa aacgacaatg gcaacaggca agacctgaag
      121 acggaaaatc aggaaaacgg tacattccac gccgtttctt ctctcggctt atccgccatt
      181 tacgatttca aactcaacga taaattcgat aaattcaaac cctatatcgg tgtgcgcgtc
      241 gcctacggac acgtcagaca cagcatcgat tcgaccaaaa aaacaacaaa tgttgttacc
      301 gtcgccggtg ctgctaacac agcacctacg atttattatg caccagagac gcaaaacgcc
      361 tatcacgaaa gccacagcat ccgccgcttg ggtcttggtg tcatcgccgg tgtcggtttc
      421 gacatcacgc ccaagctgac cctggacacc ggataccgtt accacaactg gggacgcttg
      481 gaaaacaccc gcttcaaaac ccacgaagtc tcattgggca tgcgctaccg cttctgattc
      541 cccgata
//
LOCUS       DAXHMX010000276          542 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXHMX010000276 DAXHMX010000000
VERSION     DAXHMX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.276"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>542
                     /locus_tag="ACFM4S_002163"
     CDS             <1..>542
                     /locus_tag="ACFM4S_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFC6051757.1"
                     /translation="IVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSA
                     LKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVE
                     RGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQ
                     VLAKPGTITPHTKFKAEVYVL"
BASE COUNT          144 a          135 c          144 g          119 t
ORIGIN      
        1 atcgtgttca tgaacaaatg cgacatggtc gacgatgccg agctgttgga actggttgaa
       61 atggaaatcc gcgacctgct gtccagctac gacttccccg gcgacgactg cccgattgta
      121 caaggttctg cactgaaagc cttggaaggc gatgccgctt atgaagagaa aatcttcgaa
      181 ctggctgccg cattggacag ctacatcccg actcccgagc gtgccgtgga caaaccgttc
      241 ctgctgccta tcgaagacgt attctctatt tccggtcgtg gtacagtagt aaccggtcgt
      301 gtagagcgcg gtatcatcca cgttggtgac gagatcgaaa tcgtaggtct gaaagaaact
      361 caaaaaacca cttgtaccgg tgttgaaatg ttccgcaaac tgctggacga aggtcaagca
      421 ggcgacaacg taggcgtatt gctgcgcggt accaaacgtg aagacgtaga gcgtggtcaa
      481 gtattggcta aaccgggtac aatcactcct cacaccaagt tcaaagcaga agtatacgta
      541 ct
//
LOCUS       DAXHMX010000277          522 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXHMX010000277 DAXHMX010000000
VERSION     DAXHMX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.277"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..342)
                     /locus_tag="ACFM4S_002164"
     CDS             complement(<1..342)
                     /locus_tag="ACFM4S_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051758.1"
                     /translation="MHTQRKNFSDSISLHDCYCTEIKVEGRNVCFNFEDGFVVPDNNP
                     NNQAGKHLKTDFSRVVLTHKNENAEDDYLFDIFEDIVFLGKRLFTVRKFLDFSELLEM
                     VNTQGYFLEFLY"
BASE COUNT          176 a          120 c           79 g          147 t
ORIGIN      
        1 atacagaaac tcaagaaaat aaccttgtgt attgaccatc tcaagcaatt cagaaaaatc
       61 aagaaatttt ctgaccgtaa acaaacgttt ccctaaaaaa acgatgtctt caaaaatatc
      121 gaacaaataa tcatcttcgg cgttttcatt tttatgggtt aaaacaacac gggaaaaatc
      181 tgttttcaga tgcttgcccg cttgattgtt cggattattg tccggaacga caaaaccgtc
      241 ctcaaaatta aagcagacgt tgcgtccttc tacctttatc tctgtgcaat aacaatcatg
      301 tagagaaatg ctatccgaaa aattttttct ttgtgtatgc aaaaaaagtt ttcattcaag
      361 tacccatatc taacacgaga cctttgcaaa attccccaaa atcccctaaa ttcccaccaa
      421 gacatttagg ggattttcca tgagcacctt cttccggcaa accgcacaag ccatgattgc
      481 caaacacatc gaccgtttcc cactattgaa gttggagcag gt
//
LOCUS       DAXHMX010000278          516 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXHMX010000278 DAXHMX010000000
VERSION     DAXHMX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.278"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..208
                     /locus_tag="ACFM4S_002165"
     CDS             <1..208
                     /locus_tag="ACFM4S_002165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051759.1"
                     /translation="ANQRCTGLDLIHYTCVMVSGTHHQPALHSVVLSGNCPVVSMGCP
                     AAPCAAQRLCALGGREWRPGGLLL"
BASE COUNT          113 a          119 c          145 g          139 t
ORIGIN      
        1 ggcgaaccaa cgctgtactg gtttagattt aattcactat acctgcgtaa tggtatcggg
       61 tactcatcat caacctgcct tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc
      121 gatgggttgc cctgccgctc cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcga
      181 gtggcggccg ggcggtttgt tgctatgata cgggagttcc gatatacaag ggtgggcttc
      241 agcccaccaa tcaacgaccg aaaaagaaaa aacggtgggc tgaagcccac tctacgcaat
      301 tacgcaactg ttatcgtggg gatgacggga ttttaggttt ttgtttttct gtttttgcgg
      361 gaaagatggg ttttaggttg cggtcattta tcggaaaagc agaaatcgct ccgccgtcat
      421 tcccgcgaaa gcgggaatct gaacacgtcc gtagggaaac ctatatcccg tcattcccac
      481 gaaagtggga atctagaacc tctgaatttt cagaca
//
LOCUS       DAXHMX010000279          495 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXHMX010000279 DAXHMX010000000
VERSION     DAXHMX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.279"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..272)
                     /locus_tag="ACFM4S_002166"
     CDS             complement(<1..272)
                     /locus_tag="ACFM4S_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6051760.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRA"
     gene            complement(256..>495)
                     /locus_tag="ACFM4S_002167"
     CDS             complement(256..>495)
                     /locus_tag="ACFM4S_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6051761.1"
                     /translation="MAVIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSD
                     NSEKVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          145 a          100 c           83 g          167 t
ORIGIN      
        1 gcccttgtgc tgaatttcgc gcctaagcgg gcaagtttgc cgccccccgc caatacgccg
       61 gcgcgggaaa tgctggcggt tattttggcg ttgattcggg cttttgcgcc cgtagggatg
      121 tgtgttaaat ctaccgtttt tattaaatca gatgaataag ttttactatt tttaggtaca
      181 aacttatgaa ttttcgcacc ttgtccggta tcaactgaaa cagtttcaga tatttttact
      241 gcatttgcat tcgcttcaaa cgaatacatc atcaaaattg caattatcga caatttcgca
      301 aaattcaaat ttgtatattt tatgaccatc tttcagggat tctttaatta ccttttctga
      361 attatcagaa aatgagatta gccaaatatc atgtttaatt cttctattcc agaaaaaaga
      421 gaaacaatca ataacatttt cagacttatt aatcttcgca aattcaacaa attcagattg
      481 cgctataacc gccat
//
LOCUS       DAXHMX010000280          474 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXHMX010000280 DAXHMX010000000
VERSION     DAXHMX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.280"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            1..279
                     /locus_tag="ACFM4S_002168"
                     /pseudo
     CDS             1..279
                     /locus_tag="ACFM4S_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          111 a          146 c          102 g          115 t
ORIGIN      
        1 caagccatga ttgccaaaca catcgaccgc ttcccgctat tgaagttgga ccaggtgatt
       61 gattggcagc cgatcgaaca atacctgaac cgtcaaagaa cccgttacct cagagaccac
      121 cgcggccgtc ctgcctaccc gctgttgtcc atgttcaaag ccgtcctgct cggacaatgg
      181 cacagcctct ccgttcccga actcgaacac agcctcatca cccgcatcga cttcaacctg
      241 ttttgccgtt tcgacgaact gagcatccct gattacagta tagtggatta acaaaaacca
      301 gtacggcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgtt gaagcaccaa
      361 gtgaatcggt tccgtactat ttgtactgtc tgcggcttcg tcgccttatc ctgatttttg
      421 ttaatccact ataaagaccg tcgggcatct gcagccgtca ttcccgcgcc ggcg
//
LOCUS       DAXHMX010000281          439 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXHMX010000281 DAXHMX010000000
VERSION     DAXHMX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.281"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>439
                     /locus_tag="ACFM4S_002169"
     CDS             <1..>439
                     /locus_tag="ACFM4S_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6051762.1"
                     /translation="TAGAGVGAALGLNGAAAAAADAAFASLASQASVSLINNKGDVGK
                     TLKELGRSRTVKNLVVAAATAGVSNKLGASSLATWSETPWVNNLNVNLANAGSAALIN
                     TAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHKV"
BASE COUNT          110 a          125 c          124 g           80 t
ORIGIN      
        1 ttactgcggg cgcgggagtc ggagccgcac taggcttaaa cggcgcagcc gcagcagcgg
       61 ccgatgccgc ctttgcctca ctcgcttctc aggcttccgt atcgctcatc aacaataaag
      121 gcgatgtcgg caaaaccctg aaggaactgg gcagaagccg cacggtaaaa aatctggttg
      181 tagcggcggc aacggcaggc gtatccaaca aactcggtgc ctcttccctt gccacttgga
      241 gcgaaacccc ttgggtaaac aacctcaacg ttaacctggc caatgcgggc agtgccgcgc
      301 tgatcaacac cgctgttaac ggcggcagcc tgaaagacaa tctggaggca aatatcctgg
      361 cggcattggt gaataccgcg catggggagg ctgcgagtaa gattaagcaa ttggatcagc
      421 actatatcgc ccacaaagt
//
LOCUS       DAXHMX010000282          401 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXHMX010000282 DAXHMX010000000
VERSION     DAXHMX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.282"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            108..323
                     /gene="rpmE"
                     /locus_tag="ACFM4S_002170"
     CDS             108..323
                     /gene="rpmE"
                     /locus_tag="ACFM4S_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC6051763.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          123 a          104 c           74 g          100 t
ORIGIN      
        1 attttatcga atatcctctt gacgaaacaa cagaaaatag cgagaatgcc gagcttgtct
       61 gtagcatagg tacattccct atgcccgctt ttttgaaagg aaatcaaatg aaacaaggta
      121 ttcacccgaa ctaccacgaa gtcaacgtta cctgctcttg cggcaacaaa ttcgcaacca
      181 aatccgcgat ggaaaaagaa aacttcaata tcgaggtttg ctccctgtgc cacccgttct
      241 ataccggcac ccaaaaaatc gtcgatacca ccggccgcgt ggacaaattc aacaacaaat
      301 tcggcaacct gttcaaacgc tgattgccgc tttgcaagaa ttaagccctg ccatcatttc
      361 gatggcaggg ctttttaata tttggcacac tgacttctta t
//
LOCUS       DAXHMX010000283          378 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXHMX010000283 DAXHMX010000000
VERSION     DAXHMX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.283"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT          114 a           83 c           86 g           95 t
ORIGIN      
        1 acatcccgtc attctcacga aagtgggaat ccagtttttt gagtttcagt cattttcaat
       61 aaattgcctt agcattgaat gtctagattc ccgcctgcgc gggaatgacg ggatttgagg
      121 ttgcggcatt tatcggaaaa aacagaaaac caaaaacaga aacctaaaat tcgtcattcc
      181 cgcgcaggcg ggaatccaat gcgttgagtt tcagctattt agaataaatt ttgaaactct
      241 aatcgcgtca ttcccacgaa agtgggaatc tagaaattta atgttgcggc actagccaaa
      301 aaaaccgaaa ccgaacggac tagattcccg cctgcgcggg aatgacggct gcagatgccc
      361 gacggtcttt atagtgga
//
LOCUS       DAXHMX010000284          377 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXHMX010000284 DAXHMX010000000
VERSION     DAXHMX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.284"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT          109 a           98 c           80 g           90 t
ORIGIN      
        1 gattcatctg ccagctaagg taaattatac cataaatttt aaataaatca agcggtaagt
       61 gattttccac ggccactcgg attaatccgg gtggcttcct ttttaaaggt ttgcaaggca
      121 agcggggtcg tccgttttgg tggaaaataa tatatcgact gcgcttcaag gctctgcgcg
      181 tacctcattg ccgcccgttt aaacacgtgg tttatctgac aggcgcgcaa tccgccctct
      241 catttaccga acaagcggac tagattcccg cctactcggg aatgatgaag ctatccgcac
      301 ggaaacctgc accacgtcat tcccacaagg acagaaaacc aaaaacagaa acctaaaatt
      361 cggcattccc gcaaaag
//
LOCUS       DAXHMX010000285          372 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXHMX010000285 DAXHMX010000000
VERSION     DAXHMX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.285"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(168..>372)
                     /locus_tag="ACFM4S_002171"
     CDS             complement(168..>372)
                     /locus_tag="ACFM4S_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051764.1"
                     /translation="IGGQSTAAIADLAGEMRRLAKKSKMLLKGLWPFLSSTISAFKIN
                     QSLFKHYCKTVQLLNCKHRKKNY"
BASE COUNT          112 a           68 c           76 g          116 t
ORIGIN      
        1 agagatgaaa cagcgtatcg gctccctgcc ggttgaattt tccgaaaaaa cgcgacgtaa
       61 ccagcatcaa catatataag aacagcacaa atagcatcaa tacatcaggc aacgaaaatg
      121 cagaataatg cacttaatgg tgtttggata tctgttgttt tgtgctgtta gtaattcttc
      181 tttctgtgtt tacagtttag cagttgtaca gttttacagt aatgtttaaa caatgactga
      241 tttattttaa atgcagatat tgtagaggat aaaaatggcc aaagtccttt cagtaacatt
      301 tttgattttt tagcgagcct tctcatttcc ccggcgagat cggcaatggc agcggtactt
      361 tggccgccga ta
//
LOCUS       DAXHMX010000286          369 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXHMX010000286 DAXHMX010000000
VERSION     DAXHMX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.286"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT          114 a           97 c           69 g           89 t
ORIGIN      
        1 tgggaatcta gaacgtaaaa tctcaagaaa ccgttttatc cgataagttt ccgtgccgac
       61 agacctagat tcccgcctgc gcgggaatga cgggatttga gattgcggca tttatcggga
      121 gcaacagaat ccgctctgcc gtcattccca cgaaagtggg aatccaggac acaaaatctc
      181 aagaaaccgt tttgcccgat aagtttccgc accgacagac ctagattccc gcctgcgtgg
      241 gaatgacggc ggaaagattc tatttttccc gataatcacc cacaatctca aattccttca
      301 ttctctcaaa aacaaaatca gaaccctaaa tcccatcatc cccatctatg tgaatataaa
      361 aattttaaa
//
LOCUS       DAXHMX010000287          368 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXHMX010000287 DAXHMX010000000
VERSION     DAXHMX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.287"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT          127 a           81 c           97 g           63 t
ORIGIN      
        1 ttcccgcaaa agcgggaatc tagaacctct aacgtggcag gaatctatcg gaaatgactg
       61 aaaccgaaca gactggattc ccgcctgtgc gggaatgacg aagcctgcgt gggaatgacg
      121 aaaagcaacc tttccgtgtc attcccgcaa aagcgggaat ctaggaactc aaagctgcaa
      181 gaatttatca gaaacaactg gaacttaaaa aaactagatt cccgcctgcg cgggaatgac
      241 gaagggttaa agaaatgacg agaaacggcg gtaatttatc gggaataacc gaaaacgaac
      301 ggaaatcagg acaaggcgac gaggccgcag acagtacaaa taggacggaa ccgattcact
      361 tggtgctt
//
LOCUS       DAXHMX010000288          366 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXHMX010000288 DAXHMX010000000
VERSION     DAXHMX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.288"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..>366)
                     /locus_tag="ACFM4S_002172"
     CDS             complement(<1..>366)
                     /locus_tag="ACFM4S_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051765.1"
                     /translation="VKEYQPPVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFEALIRDADFQYIFLSYNNEGLMSVGQVREIFECFGKYNLVQTEYRRFKADKT
                     ENRNHKANSIFEYLHILEKT"
BASE COUNT          123 a           87 c           59 g           97 t
ORIGIN      
        1 gtcttttcta aaatatgcag atattcgaat atcgaatttg ccttatgatt acggttttct
       61 gtcttatctg ccttaaaacg ccggtattcc gtttgaacca aattatattt gccgaaacac
      121 tcaaaaatct cccttacctg cccgacagac atcaagcctt cgttgttgta gctgagaaaa
      181 atatattgaa agtccgcatc cctaatcaaa gcctcaaata ccggaaccac atccgatttt
      241 gagcaagtcc gcgctaaaac acatcgtaca gcgcaaaacc ggatgggaat ggcaaaatcc
      301 ggaaacaaac atttttcaaa gcatacaaac aaaaaaatac caaccggagg ttggtattcc
      361 ttaact
//
LOCUS       DAXHMX010000289          351 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXHMX010000289 DAXHMX010000000
VERSION     DAXHMX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.289"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(<1..341)
                     /locus_tag="ACFM4S_002173"
     CDS             complement(<1..341)
                     /locus_tag="ACFM4S_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051766.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIF"
BASE COUNT          122 a           53 c           33 g          143 t
ORIGIN      
        1 aagaaaatat aagccttata atatttcttt tcaaaaaaat caaaactttc ttctttcaac
       61 cagtccatat aactgtcatc tattatctcg aaaaaataat ttatagcagg atttctatcc
      121 caatcatttt tttctttatt ttcaaataaa tattctgaaa attttacttt ataagattca
      181 tctgttattt gaaaatgaat tatattgtca ataaatattt caatatttaa ttcataattt
      241 cggtcggatg ccaaaatgtt cacatcttgt tcggtaacac aaattttgtc tacgtaaaat
      301 ctgcgactat tgttagaaaa ttgtaatggt ttaatcttca ctatcgtttt c
//
LOCUS       DAXHMX010000290          332 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXHMX010000290 DAXHMX010000000
VERSION     DAXHMX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.290"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT           96 a           73 c           71 g           92 t
ORIGIN      
        1 tttttgaatt tcgggcaacg cttgaatctt cattccgcgc aggcggaaat tatcggtgcg
       61 gtacggcaac ttttttcgat atgaaaagac cgtcattcct gtaaaaacaa aaaatcaaaa
      121 acagaaaatt gaaattcgtc attcccgcgc aggcgggaat ctaggacgta aaatctatag
      181 tggattaaca aaaaccagta cagcgttgcc ttgacttagc tcgaagagaa cgattctcta
      241 aggtgctgaa gcaccgagtg agtcggttcc gcactatccg tactgtctgc ggcttcgtcg
      301 ctttctcctg atttttgtta atccactata aa
//
LOCUS       DAXHMX010000291          331 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXHMX010000291 DAXHMX010000000
VERSION     DAXHMX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.291"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..192
                     /locus_tag="ACFM4S_002174"
     CDS             <1..192
                     /locus_tag="ACFM4S_002174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051767.1"
                     /translation="GIQQPKNHRNLSAKTETPTASFPQKLESKNATQQEFIGKNRNPT
                     DRHSRKSGNPDPSARNLTG"
BASE COUNT          123 a           92 c           61 g           55 t
ORIGIN      
        1 ggaatccagc aaccgaaaaa ccacaggaat ctatcagcaa aaacagaaac ccccaccgcg
       61 tcattcccgc aaaagctgga atccaaaaac gcaacgcaac aggaatttat cggaaaaaac
      121 agaaacccca ccgaccgtca ttcccgcaaa agcgggaatc cagacccgtc ggcacggaat
      181 cttaccggat aaaacagttt ccttagattc cacgtcctag attcccgcct tcgcgggaat
      241 gacgagattt tagattatgg gaatttatca ggaatgattg aatccataga aaaaccacag
      301 gaatctatca gaaaaaacag aaacccccac c
//
LOCUS       DAXHMX010000292          317 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXHMX010000292 DAXHMX010000000
VERSION     DAXHMX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.292"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..90
                     /locus_tag="ACFM4S_002175"
     CDS             <1..90
                     /locus_tag="ACFM4S_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6051768.1"
                     /translation="DVYETFKEDIQARGYQYDPETDKFAKVSG"
BASE COUNT           99 a           60 c           93 g           65 t
ORIGIN      
        1 gacgtatacg aaactttcaa agaagacata caggcacgag gctaccaata cgaccccgaa
       61 accgacaaat ttgcaaaggt ctcaggctaa gtgcgcctgt tgccgcctaa aaggcggtcc
      121 ggatgcctga ttatcgggta tccggggagg attaagggga tatttgggta gaattaggag
      181 gtgttatgga gcgaaaacag ccgaaaacct gtgtttgggt ttcggctgtc gggagggaaa
      241 ggaattttgc aaaggtctca attagtatag tggattaaca aaaatcagga caaggcgacg
      301 aagccgcaga cagtaca
//
LOCUS       DAXHMX010000293          315 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXHMX010000293 DAXHMX010000000
VERSION     DAXHMX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.293"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..90
                     /locus_tag="ACFM4S_002176"
     CDS             <1..90
                     /locus_tag="ACFM4S_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6051769.1"
                     /translation="DVYETFKEDIQARGYQYDTETDKFAKVSG"
BASE COUNT           89 a           67 c           85 g           74 t
ORIGIN      
        1 gacgtatacg aaactttcaa agaagacata caggcacgag gctaccaata cgacaccgaa
       61 accgacaaat ttgcaaaggt ctcaggctaa gtgcgcctgt tgccgcctaa aaagtaaccc
      121 ggatgcctga ttatcgggta tccggggagg attaaggggg tatttaggta aaattaggag
      181 gtatttgggg cgaaaacagc cgaaaacctg tgtttgggtt tcggctgtcg ggagggaaag
      241 gaattttgca aaggtctctt ttcgtcattc ccgccacttt tcgtcattcc cgcgaaagcg
      301 ggaatctaga atctc
//
LOCUS       DAXHMX010000294          313 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXHMX010000294 DAXHMX010000000
VERSION     DAXHMX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.294"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            68..>313
                     /locus_tag="ACFM4S_002177"
     CDS             68..>313
                     /locus_tag="ACFM4S_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6051770.1"
                     /translation="MSYTQLTQGERYHIQYLSRHCTVTEIAKQLNRHKSTISREIRRH
                     RTQGQQYSAEKAQRQSQTIKQRKRQPYKLDSQLIQHID"
BASE COUNT          104 a          105 c           62 g           42 t
ORIGIN      
        1 caactttccc taacagaaaa aggccagtat gcggtagcat acggcctttc ctgcaagaaa
       61 gattgccatg agctacacgc aactgaccca aggcgaacga taccacatcc aatacctgtc
      121 ccgccactgc accgtcaccg aaatcgccaa acagcttaac cgccacaaaa gcaccatcag
      181 ccgcgaaatc agacggcacc gcacccaagg gcagcaatac agcgccgaaa aagcccagcg
      241 gcaaagccag actatcaaac agcgtaagcg acaaccctat aagctcgatt cgcagctgat
      301 tcagcacatc gac
//
LOCUS       DAXHMX010000295          305 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXHMX010000295 DAXHMX010000000
VERSION     DAXHMX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.295"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>305
                     /locus_tag="ACFM4S_002178"
     CDS             <1..>305
                     /locus_tag="ACFM4S_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6051771.1"
                     /translation="RYRNWLAQDNTLSELLELINRQLTEKGLKVEKASAAVIDATIIQ
                     TAGSKQRQAIEVDEEGQVSGQTTLSKDKNARWTKKNGLYKLGYKQHTRTDAEGYIEKL
                     "
BASE COUNT          105 a           83 c           70 g           47 t
ORIGIN      
        1 cgctaccgca actggttggc gcaagacaac accctgtccg aattattgga actgatcaac
       61 cgccaactga ccgaaaaagg cttaaaagta gagaaagcat ccgccgccgt cattgatgcc
      121 accattattc agactgccgg cagcaaacag cgccaggcca tagaagtcga cgaggaagga
      181 caagtcagcg gtcaaaccac actgagtaag gacaaaaatg cccgttggac aaagaaaaac
      241 ggcctctaca aactcggtta caaacaacat acccgtaccg atgcggaagg ctatatcgag
      301 aaact
//
LOCUS       DAXHMX010000296          296 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXHMX010000296 DAXHMX010000000
VERSION     DAXHMX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.296"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT           80 a           81 c           73 g           62 t
ORIGIN      
        1 cctttgaata ttgccgccgt cccgcgttct agattcccgc ctgcgcggga atgacgattc
       61 atcagtttcc cgaaacgccc gaaaaaccga aaccgagcaa accggattcc cgcctgcgcg
      121 ggaatgacga tttataagtt tcccgaaacg cccaaaaaaa ccgaaatccg acaggcagga
      181 tttttgcttt cgtggggatg acgggatttt aagttacggt catttatcgg aaaagcagaa
      241 actgctccgc cgttattcca gcgaaggcgg gaatctgaac acgtccgcac ggaaac
//
LOCUS       DAXHMX010000297          290 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXHMX010000297 DAXHMX010000000
VERSION     DAXHMX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.297"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT           72 a           70 c           80 g           68 t
ORIGIN      
        1 tgcaggtttc cgtacggata ggttcgtcat tcccgcgcag gcgggaatct agacattcaa
       61 tactaaggca atttatcggg aatgactgaa actcaaaaaa ctggattccc actttcgtgg
      121 gaatgacggg atgcaggttc gtgggaatga cgtggtgcag gtttccgtac ggatggattc
      181 gtcattcccg cgcaggcggg aatccagacc tgtccgcaca gaaacttatc ggataaaaac
      241 agttgctcca accccgcgtc ctagattccc actttcgtgg gaatgacggg
//
LOCUS       DAXHMX010000298          287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXHMX010000298 DAXHMX010000000
VERSION     DAXHMX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.298"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>287
                     /locus_tag="ACFM4S_002179"
     CDS             <1..>287
                     /locus_tag="ACFM4S_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6051772.1"
                     /translation="HITEVKEHKNAKPDAVNLSASQGIDIKSGGSIDAYATAFDAPKG
                     SINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALP"
BASE COUNT           98 a           94 c           58 g           37 t
ORIGIN      
        1 cacattaccg aagtcaaaga acacaaaaac gccaagcccg acgcagtaaa cctcagcgca
       61 tcccaaggca tcgacatcaa atctggtggc agcatcgacg cctacgccac cgcattcgat
      121 gcccccaaag gcagcattaa catcgaagcc gggcggaaat tgacactcta tgccgtagaa
      181 gagctcaact acgacaaact agacagccaa aaaaggcgca gatttctcgg catcagctac
      241 agcaaagcac acgacaccac cacccaagtc atgaaaaccg cgctgcc
//
LOCUS       DAXHMX010000299          286 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXHMX010000299 DAXHMX010000000
VERSION     DAXHMX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.299"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT           85 a           83 c           78 g           40 t
ORIGIN      
        1 gcctgcgcgg gaatgacgaa aagcaacctt tccgcgtcct tcccgcgaaa gcgggaatct
       61 agaaccccta acgggcagga atctatcgga aaaaaccgaa accgaacaga ctggattccc
      121 gcctgcgcgg gaatgacgaa gcctgcgcgg gaatgacgaa aagcaacctt tccgcgtcat
      181 tctcgcgaaa gcgggaatct agaaccccta acgcggcagg aatctatcgg aaaaaaccga
      241 aaccgaacag actggattcc cgcctgcgcg ggaatgacga agcctg
//
LOCUS       DAXHMX010000300          280 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXHMX010000300 DAXHMX010000000
VERSION     DAXHMX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.300"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            complement(1..107)
                     /locus_tag="ACFM4S_002180"
                     /pseudo
     CDS             complement(1..107)
                     /locus_tag="ACFM4S_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT           65 a           54 c           75 g           86 t
ORIGIN      
        1 atgtcggtgg aggttgccac gccggcagaa ttgttgttgg cgggccatat gccgtgattc
       61 aggtaatact cggtaacggc tgatttttga ccttcagcca aaagaatgac gaattttagg
      121 tttctgtttt tggttttctg tccttgtggg aatgatgaaa ttttaagttt taggaattta
      181 tcgaaaaaac agaaaccgct ccgccgtcat tcccgcgcag gcgggaatcc agcctcgtcg
      241 gtgcggaaac ttatcgggta aaacggtttc ttgagatttt
//
LOCUS       DAXHMX010000301          277 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXHMX010000301 DAXHMX010000000
VERSION     DAXHMX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.301"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..180
                     /locus_tag="ACFM4S_002181"
     CDS             <1..180
                     /locus_tag="ACFM4S_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6051773.1"
                     /translation="LAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILF
                     PKFGEYDLVLNPDII"
BASE COUNT          103 a           28 c           53 g           93 t
ORIGIN      
        1 ttagcagaag gatatactta tttcttcaat gaaaatacgg atgattgcta tgttttgaaa
       61 gaggatttta ttaataatga gcgatatgaa aaaacagaat tatattccca aaaagataag
      121 gtaattctat ttccaaagtt tggtgaatat gatttggtgt taaatccgga cattatttaa
      181 ttaagtttta aggccgtctg aaaaaaattt caaacggctt ttattattgg gtttggaatc
      241 tgaggataaa gctgataaaa accaggaaat tatcagg
//
LOCUS       DAXHMX010000302          275 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXHMX010000302 DAXHMX010000000
VERSION     DAXHMX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.302"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT           89 a           86 c           40 g           60 t
ORIGIN      
        1 tatcccgtca ttcctacgaa cctacatccc gtcattccct caaaaacaga aaaccaaaat
       61 tagaaaccta aaatcccgtc attcccgcgc aggcgggaat ccaaacttgt ccgcacggaa
      121 acttatcgga taaaacggtt tcttagattc cacgttctag attcccgcct gcgcgggaat
      181 gacgaatcca tccgcacgga aacctatatc ccgtcattct tacgaaccta catcccgtca
      241 ttccctcaaa aacagaaaac caaaattaga aacct
//
LOCUS       DAXHMX010000303          269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXHMX010000303 DAXHMX010000000
VERSION     DAXHMX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.303"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..264
                     /locus_tag="ACFM4S_002182"
     CDS             <1..264
                     /locus_tag="ACFM4S_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6051774.1"
                     /translation="FYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQT
                     DFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT           89 a           67 c           51 g           62 t
ORIGIN      
        1 ttctaccaac acaccaaaat aaccaaagca ttgaaagcgg agacttattt ttgtcgccct
       61 taccattctt gggagaaagg gctgaatgag aacaccaacg gactcatccg gcaatacttc
      121 cccaaacaaa ccgatttccg taacatcagt gatcgggaga tacgcagggt tcaagatgag
      181 ttgaaccacc gaccaagaaa aacacttggc tacgaaacgc caagtgtttt attcttgaat
      241 ctgttccaac cactaataca ctagtgttg
//
LOCUS       DAXHMX010000304          263 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXHMX010000304 DAXHMX010000000
VERSION     DAXHMX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.304"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT           57 a           60 c           73 g           73 t
ORIGIN      
        1 cggctcgatg aatcgtcaga aatacccgaa ccgtcattcc cgcgcaggcg ggaatctaga
       61 ccggtcggtg cggaaactta tcgggaaaaa aggtttctcc gtccctgaat cctggattcc
      121 cactttcgtg ggaatgacag aatgtaggtt cgtgggaatg acgtggtgca ggtttccgtg
      181 cggatgaatt catcattccc gcgcaggcgg gaatctagtc tgttcggttt cagttatttt
      241 cgataaatgc ttgttgcttt tca
//
LOCUS       DAXHMX010000305          261 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXHMX010000305 DAXHMX010000000
VERSION     DAXHMX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.305"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
     gene            <1..>261
                     /locus_tag="ACFM4S_002183"
     CDS             <1..>261
                     /locus_tag="ACFM4S_002183"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFC6051775.1"
                     /translation="IRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAE
                     LDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNL"
BASE COUNT           63 a           85 c           61 g           52 t
ORIGIN      
        1 atccgaaaca cccaagattc cgtcgtcaac ctcgacaaac tgacctacgc gggcaatctc
       61 gaatcgctga ccgacattgc cgataatccc cgctatgctt tcgagcaagt ggacatttgc
      121 gaccgcgccg aactcgaccg cgtattcgca caacaccggc ccgatgccgt gatgcacttg
      181 gcggcggaaa gccatgtcga ccgctctatc ggttcggcag gcgagtttat ccaaaccaat
      241 atcgtcggca cattcaatct g
//
LOCUS       DAXHMX010000306          259 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 241930
            SAMEA1706811-rid23337773.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXHMX010000306 DAXHMX010000000
VERSION     DAXHMX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706811
            Sequence Read Archive: ERR484239
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378227.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378227.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:23:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,183
            CDSs (total)                      :: 2,125
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 50 of 125
            Pseudo Genes (incomplete)         :: 85 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 25 of 125
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706811-rid23337773.denovo.306"
                     /strain="M01 241930"
                     /db_xref="taxon:487"
BASE COUNT           77 a           63 c           62 g           57 t
ORIGIN      
        1 atccgtacgg aaacctgcac cgcgtcattc ccacgaaaga gggaatccaa acttgtccgc
       61 acggaaactt atcggataaa acggtttctt agattccatg ttctagattc ccgcctgcgc
      121 gggaatgacg gattttaggt tgggggcatt tattgggaaa agcagaaacc gctccgccgt
      181 cattcccacg aaagtgggaa tctagaaatt taatgttgcg gcactagcca aaaaaaccga
      241 aaccgaacgg actagattc
//
